CmaCh10G007900 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh10G007900
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
DescriptionPectinesterase
LocationCma_Chr10: 3634710 .. 3637885 (+)
RNA-Seq ExpressionCmaCh10G007900
SyntenyCmaCh10G007900
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATTCCCATTTTACCCCTCGCGTTTATAAATATCTACCACCACGGTCTCCCACCTCGAATTCATTCAAGGCCAAAAACCGCCCCCACAAGGAGAGTTTTGGGAGAATGTCTCGGCCAGCCGTTGCTTCATCTTTTCCGGCGACGGTGGCGCTCGTACTGGCGGTGGTACAACTCATCACCATGCTTATTCCTGCAGCTATTGTCGTCGAAGCTCGTACGAAGCCGATTCCGGCAGAGCCATCGCAGCTCGACGAATGGATCGCACACAACATGAGAGAGTACCGTCAACGGAAGGCCAAAGAGACAGGAGCCAAGGCGCTGGATCGGCGGCTTGCTAAGGCGGAGGATTGCGCTCGACTGATCACCGTTAAACAAGACGGACAAGGAAATTTCTCAACGATCACCCAAGCGATCGAGAGCATTCCGTCCGGAAATAGTGAGCGAGTGGTGGTGTGGATCGGCGGTGGAACTTACCGCGAGAAGATCACGATCGATTCTTCGAAGCCGTTCGTGACGCTGTACGGTCAGACAGGAAAAATGCCGGTCATATCGTACGACGGAACGGCGTCGGAATTTGGTACAGTCGAGAGCGCGACNGTGGCGGTGGAATCGGATTATTTCGTCGCCGTCAATGTTGCGTTTGTGGTGAGTTTCTCTTCTTCTTCTCCTCATTTTTGTGGGGTAAGTTTTGTAAATAGTGAAAGTTATGGGGCGCAAAAAAGTTTTAGTAACGTATTATTGCGTAAGTACTAATTAATTATTTATTTATATTTTTTTCCGGAAAAAATTCATGCATTTATTTGTCATCAAAATTAAGCACGAGTTTTCACGTTGAAATCTTGACATTTTCTTGCACAATTGGTTATTTCTGTTAGAAAAGTTCCCTTTTGAGATGACTATACCTTGTTGGATAAAAGATTTGTAGGTTTTTAAATCACTCATGTTGATTGGAATGAGATTCCTGCTTAGAAATCTTGTTCGGTGTAGATTCGAAAATATGTAGTTTCATTTTAGATAACAATCTGTCATGATTTGGTTGTCAGGATCGTTCAATTGATATATAGGATGTATCATCGATTAAGATGTCAAAAGTTTGAATCTATGTAATAAAAAAAATGCATAAATTGGTAAGAAATTTAATACTTATTATCGTACAATTGCAACCACGATTGGAGAGGAGGATGAAACATTACATTCCTAGTGTGGAAACCTCATCCTAATAGATGTGCTATATTCCTCTTAGGGGTGTGGAAACAACCTCTCATTAGTTTAGTAGATGTGCCATATCAGTTGGAGAAGAGCTAAACATTCATTTACAAGGGGTTGGAAACCTCTCTCCACGTATTAAAAACCTTGAGGGAACCCCGAGAGGGAAAGTCCAAGAGGACAATATCTGCTAGTGGTAGGCTTGACTGTTACAAATGGTATCGGAGATAGACACCAGGCTGTGTGCTAGCGAGGACCCTGAGCCCCTAAGGAAGTGAATTGTGAGATTCCACGTTAGTTCGATTCCACGTTAGTTCGATTCCACGTTAGTTCGATTCCACACTAACCTTTCGAGTTCAAAATTGTACGATCTCATACCGTGTTTTGAATGAAAATGAAATTGTAGTGAATAACAAGAAATGAAGATAGGGTATGATGAATTTGTTGATGCAGAATTCAGCTCCAATGCCGGATGCGGGAAGAACAGGAGGACAAGCAGTGGCAATGAGGATATCAGGGGATAAGGCAGCATTCCACCACTGCCAATTCATAGGGTTTCAGGACACTTTATGCGATGACAGAGGCAGACATTTTTTCAAGGATTGTTACATCGAAGGCACTGTGGACTTCATCTTTGGAAATGGCAAATCCCTCTACTTGGTAAAAATCTCCTCCAATCTTCCTACACTTACAAATTAACTAACCCAACTTTTAACACAAAAATATAATCAATATTTTCTCTTACATTACCTAATTCTTCGTATAAACCATCATGATCATCTATCTGGTAAGTGGGTATTTATTTTTACTATCATTGATAAGTAGGTATCTATTTTTCTACTCACAAAGAAATTATTCATTACCTAATTCTTCATCTAAAATTTCTATCAACGGTTAATCCATCACGATCATCGAGCTGGTGAGTAGGTATCTATTTTTACTCGTGAACAAATTATTCAATAAGTCTGTTACAATGTGGTCGACCATTGTTGATCTAACCGATGTTGTTGGAGGTACTATGCAAAAATATTCCAAATTTATATCCATTTGTTGTCATCAACCATTTCATCCATATATGTGTATGTGTTTGTGACAGAAAACAGTAATAAATTCAGTGGCGGAAGGGACAGGAGTGATCACAGCTCAAGCAAGGGAAAATTCCACAGACGAAAGCGGCTTCACATTTGCATATTGCAACATCACTGGAACTGGAAACACCTATTTGGGAAGAGCATGGAAGGAGAGAACTCGTGTGGTTTTTGCTTACACTTACATGGGAACTCTCATTAACACCCAAGGATGGTCCGATAAAATGCAGGGAACCGAACCTCGAAAGTGAGCAAAACCACGCACATATCATCATCATCATCATCGTCACTATCACCTTATTGCTAATTGATTGATTAATTTTGATTATCGACCGTGTTGACAGGAGTATGTACTACGGAGAGTACAAATGCAAAGGACCCGGGGCAGACAGCACAGGACGAGCACACTACACGAGAATACTCACGGACGACGAAGCGAAGGCGTTTTTGAGTATGACTTATATCCATGGAAACAAATGGGTTCTACCACCTCCAAGGCTGTCCCTTCTCCCGGTGTCATCTCAATGCCATGGCTGATTATGTTGTCTCATGTTTGTTTTTGGATAAAGTTTTGGTTGATTCTTGTTGTTGTTGTTCCAAACCGATCAATTTCTTGAGCTTTCCAGGAAGCCTCTCCTCTCTCTCAGTTGATCTCTTCTATTAATCATTTATATGTTCTTATATTTACAAACAATGCTGCGCGCAGTTTTGCAAGTCTAGGCAAAAATATGATCATGAATGGGCCTTTGCCGACGCATTTGTGTACCATTTTTTTTATTCTTCCATCACTAATAATTGTGTACGGACAAGTGTTCAACCAATACACGTGATGAACGACTCTAACTTGAACATAAATTGTTACGATACAAGTCCACCGCTAACA

mRNA sequence

ATTCCCATTTTACCCCTCGCGTTTATAAATATCTACCACCACGGTCTCCCACCTCGAATTCATTCAAGGCCAAAAACCGCCCCCACAAGGAGAGTTTTGGGAGAATGTCTCGGCCAGCCGTTGCTTCATCTTTTCCGGCGACGGTGGCGCTCGTACTGGCGGTGGTACAACTCATCACCATGCTTATTCCTGCAGCTATTGTCGTCGAAGCTCGTACGAAGCCGATTCCGGCAGAGCCATCGCAGCTCGACGAATGGATCGCACACAACATGAGAGAGTACCGTCAACGGAAGGCCAAAGAGACAGGAGCCAAGGCGCTGGATCGGCGGCTTGCTAAGGCGGAGGATTGCGCTCGACTGATCACCGTTAAACAAGACGGACAAGGAAATTTCTCAACGATCACCCAAGCGATCGAGAGCATTCCGTCCGGAAATAGTGAGCGAGTGGTGGTGTGGATCGGCGGTGGAACTTACCGCGAGAAGATCACGATCGATTCTTCGAAGCCGTTCGTGACGCTGTACGGTCAGACAGGAAAAATGCCGGTCATATCGTACGACGGAACGGCGTCGGAATTTGGTACAGTCGAGAGCGCGACNGTGGCGGTGGAATCGGATTATTTCGTCGCCGTCAATGTTGCGTTTGTGAATTCAGCTCCAATGCCGGATGCGGGAAGAACAGGAGGACAAGCAGTGGCAATGAGGATATCAGGGGATAAGGCAGCATTCCACCACTGCCAATTCATAGGGTTTCAGGACACTTTATGCGATGACAGAGGCAGACATTTTTTCAAGGATTGTTACATCGAAGGCACTGTGGACTTCATCTTTGGAAATGGCAAATCCCTCTACTTGAAAACAGTAATAAATTCAGTGGCGGAAGGGACAGGAGTGATCACAGCTCAAGCAAGGGAAAATTCCACAGACGAAAGCGGCTTCACATTTGCATATTGCAACATCACTGGAACTGGAAACACCTATTTGGGAAGAGCATGGAAGGAGAGAACTCGTGTGGTTTTTGCTTACACTTACATGGGAACTCTCATTAACACCCAAGGATGGTCCGATAAAATGCAGGGAACCGAACCTCGAAAGAGTATGTACTACGGAGAGTACAAATGCAAAGGACCCGGGGCAGACAGCACAGGACGAGCACACTACACGAGAATACTCACGGACGACGAAGCGAAGGCGTTTTTGAGTATGACTTATATCCATGGAAACAAATGGGTTCTACCACCTCCAAGGCTGTCCCTTCTCCCGGTGTCATCTCAATGCCATGGCTGATTATGTTGTCTCATGTTTGTTTTTGGATAAAGTTTTGGTTGATTCTTGTTGTTGTTGTTCCAAACCGATCAATTTCTTGAGCTTTCCAGGAAGCCTCTCCTCTCTCTCAGTTGATCTCTTCTATTAATCATTTATATGTTCTTATATTTACAAACAATGCTGCGCGCAGTTTTGCAAGTCTAGGCAAAAATATGATCATGAATGGGCCTTTGCCGACGCATTTGTGTACCATTTTTTTTATTCTTCCATCACTAATAATTGTGTACGGACAAGTGTTCAACCAATACACGTGATGAACGACTCTAACTTGAACATAAATTGTTACGATACAAGTCCACCGCTAACA

Coding sequence (CDS)

ATGTCTCGGCCAGCCGTTGCTTCATCTTTTCCGGCGACGGTGGCGCTCGTACTGGCGGTGGTACAACTCATCACCATGCTTATTCCTGCAGCTATTGTCGTCGAAGCTCGTACGAAGCCGATTCCGGCAGAGCCATCGCAGCTCGACGAATGGATCGCACACAACATGAGAGAGTACCGTCAACGGAAGGCCAAAGAGACAGGAGCCAAGGCGCTGGATCGGCGGCTTGCTAAGGCGGAGGATTGCGCTCGACTGATCACCGTTAAACAAGACGGACAAGGAAATTTCTCAACGATCACCCAAGCGATCGAGAGCATTCCGTCCGGAAATAGTGAGCGAGTGGTGGTGTGGATCGGCGGTGGAACTTACCGCGAGAAGATCACGATCGATTCTTCGAAGCCGTTCGTGACGCTGTACGGTCAGACAGGAAAAATGCCGGTCATATCGTACGACGGAACGGCGTCGGAATTTGGTACAGTCGAGAGCGCGACNGTGGCGGTGGAATCGGATTATTTCGTCGCCGTCAATGTTGCGTTTGTGAATTCAGCTCCAATGCCGGATGCGGGAAGAACAGGAGGACAAGCAGTGGCAATGAGGATATCAGGGGATAAGGCAGCATTCCACCACTGCCAATTCATAGGGTTTCAGGACACTTTATGCGATGACAGAGGCAGACATTTTTTCAAGGATTGTTACATCGAAGGCACTGTGGACTTCATCTTTGGAAATGGCAAATCCCTCTACTTGAAAACAGTAATAAATTCAGTGGCGGAAGGGACAGGAGTGATCACAGCTCAAGCAAGGGAAAATTCCACAGACGAAAGCGGCTTCACATTTGCATATTGCAACATCACTGGAACTGGAAACACCTATTTGGGAAGAGCATGGAAGGAGAGAACTCGTGTGGTTTTTGCTTACACTTACATGGGAACTCTCATTAACACCCAAGGATGGTCCGATAAAATGCAGGGAACCGAACCTCGAAAGAGTATGTACTACGGAGAGTACAAATGCAAAGGACCCGGGGCAGACAGCACAGGACGAGCACACTACACGAGAATACTCACGGACGACGAAGCGAAGGCGTTTTTGAGTATGACTTATATCCATGGAAACAAATGGGTTCTACCACCTCCAAGGCTGTCCCTTCTCCCGGTGTCATCTCAATGCCATGGCTGA

Protein sequence

MSRPAVASSFPATVALVLAVVQLITMLIPAAIVVEARTKPIPAEPSQLDEWIAHNMREYRQRKAKETGAKALDRRLAKAEDCARLITVKQDGQGNFSTITQAIESIPSGNSERVVVWIGGGTYREKITIDSSKPFVTLYGQTGKMPVISYDGTASEFGTVESATVAVESDYFVAVNVAFVNSAPMPDAGRTGGQAVAMRISGDKAAFHHCQFIGFQDTLCDDRGRHFFKDCYIEGTVDFIFGNGKSLYLKTVINSVAEGTGVITAQARENSTDESGFTFAYCNITGTGNTYLGRAWKERTRVVFAYTYMGTLINTQGWSDKMQGTEPRKSMYYGEYKCKGPGADSTGRAHYTRILTDDEAKAFLSMTYIHGNKWVLPPPRLSLLPVSSQCHG
Homology
BLAST of CmaCh10G007900 vs. ExPASy Swiss-Prot
Match: Q9FKF3 (Putative pectinesterase 63 OS=Arabidopsis thaliana OX=3702 GN=PME63 PE=3 SV=2)

HSP 1 Score: 354.4 bits (908), Expect = 1.7e-96
Identity = 171/364 (46.98%), Postives = 243/364 (66.76%), Query Frame = 0

Query: 17  VLAVVQLITMLIPAAIVVEARTKPIPAEPSQLDEWIAHNMREYRQRKAKETGAKALDRRL 76
           V  +V ++ ++I + +V      PIP    ++++W   N                     
Sbjct: 6   VSLIVTILLVVITSPVVFGNDAAPIPENKGRIEQWFNTN--------------------- 65

Query: 77  AKAEDCARLITVKQDGQGNFSTITQAIESIPSGNSERVVVWIGGGTYREKITIDSSKPFV 136
                      VKQ+G+G+F TIT+AI S+ +GN+ RV++ IG G Y+EK+TID SKPF+
Sbjct: 66  -----------VKQNGRGHFKTITEAINSVRAGNTRRVIIKIGPGVYKEKVTIDRSKPFI 125

Query: 137 TLYGQTGKMPVISYDGTASEFGTVESATVAVESDYFVAVNVAFVNSAPMPDAGRTGGQAV 196
           TLYG    MPV+++DGTA+++GTV+SAT+ V SDYF+AVN+   NSAPMPD  R G QA+
Sbjct: 126 TLYGHPNAMPVLTFDGTAAQYGTVDSATLIVLSDYFMAVNIILKNSAPMPDGKRKGAQAL 185

Query: 197 AMRISGDKAAFHHCQFIGFQDTLCDDRGRHFFKDCYIEGTVDFIFGNGKSLYLKTVINSV 256
           +MRISG+KAAF++C+F G+QDT+CDD G HFFKDCYIEGT DFIFG+G+SLYL T +N V
Sbjct: 186 SMRISGNKAAFYNCKFYGYQDTICDDTGNHFFKDCYIEGTFDFIFGSGRSLYLGTQLNVV 245

Query: 257 AEGTGVITAQARENSTDESGFTFAYCNITGTG-NTYLGRAWKERTRVVFAYTYMGTLINT 316
            +G  VITA A +++ ++SG++F +C +TGTG   YLGR+W    +VV+AYT M +++N 
Sbjct: 246 GDGIRVITAHAGKSAAEKSGYSFVHCKVTGTGTGIYLGRSWMSHPKVVYAYTDMSSVVNP 305

Query: 317 QGWSDKMQGTEPRKSMYYGEYKCKGPGADSTGRAHYTRILTDDEAKAFLSMTYIHGNKWV 376
            GW +  +     K+++YGEYKC G G+    R  YT+ + D EAK F+S+ YI G+ W+
Sbjct: 306 SGWQENREAGRD-KTVFYGEYKCTGTGSHKEKRVKYTQDIDDIEAKYFISLGYIQGSSWL 336

Query: 377 LPPP 380
           LPPP
Sbjct: 366 LPPP 336

BLAST of CmaCh10G007900 vs. ExPASy Swiss-Prot
Match: Q9LY17 (Probable pectinesterase 50 OS=Arabidopsis thaliana OX=3702 GN=PME50 PE=2 SV=1)

HSP 1 Score: 352.4 bits (903), Expect = 6.4e-96
Identity = 171/357 (47.90%), Postives = 247/357 (69.19%), Query Frame = 0

Query: 26  MLIPAAIVVEARTKPIPAEPSQLDEWIAHNMREYRQRKAKETGAKALDRRLAKAEDCARL 85
           ++  + +V+   T PIP   +Q+ +W   N++ Y QRK        LD  L  AE   ++
Sbjct: 13  VVFASPVVLATDTDPIPENRAQIPQWFKTNVKPYSQRKG------TLDPALEAAEAARQI 72

Query: 86  ITVKQDGQGNFSTITQAIESIPSGNSERVVVWIGGGTYREKITIDSSKPFVTLYGQTGKM 145
           ITV Q G  NF T+ +AI+SIP+GN  RV++ +  G Y EK+TID ++PF+TL GQ G  
Sbjct: 73  ITVNQKGGANFKTLNEAIKSIPTGNKNRVIIKLAPGVYNEKVTIDIARPFITLLGQPGAE 132

Query: 146 PVISYDGTASEFGTVESATVAVESDYFVAVNVAFVNSAPMPDAGRTGGQAVAMRISGDKA 205
            V++Y GTA+++GTVESAT+ V ++YF A ++   N+APMP  G + GQA+AMRI+ DKA
Sbjct: 133 TVLTYHGTAAQYGTVESATLIVWAEYFQAAHLTIKNTAPMPKPG-SQGQALAMRINADKA 192

Query: 206 AFHHCQFIGFQDTLCDDRGRHFFKDCYIEGTVDFIFGNGKSLYLKTVINSVAEGTGVITA 265
           AF+ C+F GFQDTLCDD+G HFFKDCYIEGT DFIFG G SLYL T +++V +G  VITA
Sbjct: 193 AFYSCRFHGFQDTLCDDKGNHFFKDCYIEGTYDFIFGRGASLYLNTQLHAVGDGLRVITA 252

Query: 266 QARENSTDESGFTFAYCNITGTG-NTYLGRAWKERTRVVFAYTYMGTLINTQGWSDKM-Q 325
           Q R+++T+++G+TF +C +TGTG   YLGR+W    +VV+A+T M +++N  GW + + +
Sbjct: 253 QGRQSATEQNGYTFVHCKVTGTGTGIYLGRSWMSHPKVVYAFTEMTSVVNPSGWRENLNR 312

Query: 326 GTEPRKSMYYGEYKCKGPGADSTGRAHYTRILTDDEAKAFLSMTYIHGNKWVLPPPR 381
           G +  K+++YGEYKC GPG+    R  YT+ +  +E   FL++ YI G+ W+LPPP+
Sbjct: 313 GYD--KTVFYGEYKCFGPGSHLEKRVPYTQDIDKNEVTPFLTLGYIKGSTWLLPPPK 360

BLAST of CmaCh10G007900 vs. ExPASy Swiss-Prot
Match: Q9LY19 (Probable pectinesterase 48 OS=Arabidopsis thaliana OX=3702 GN=PME48 PE=2 SV=2)

HSP 1 Score: 349.7 bits (896), Expect = 4.2e-95
Identity = 169/343 (49.27%), Postives = 235/343 (68.51%), Query Frame = 0

Query: 40  PIPAEPSQLDEWIAHNMREYRQRKAKETGAKALDRRLAKAEDCARLITVKQDGQGNFSTI 99
           PIP    Q+ +W   ++    QR       K LD  L  AE   R+I V   G G F T+
Sbjct: 28  PIPEGKPQVVQWFNTHVGPLAQR-------KGLDPALVAAEAAPRIINVNPKG-GEFKTL 87

Query: 100 TQAIESIPSGNSERVVVWIGGGTYREKITIDSSKPFVTLYGQTGKMPVISYDGTASEFGT 159
           T AI+S+P+GN++RV++ +  G YREK+TID +KPF+TL GQ   MPVI+YDGTA+++GT
Sbjct: 88  TDAIKSVPAGNTKRVIIKMAHGEYREKVTIDRNKPFITLMGQPNAMPVITYDGTAAKYGT 147

Query: 160 VESATVAVESDYFVAVNVAFVNSAPMPDAGRTGGQAVAMRISGDKAAFHHCQFIGFQDTL 219
           V+SA++ + SDYF+AVN+   N+AP PD    G QA++MRISG+ AAF++C+F GFQDT+
Sbjct: 148 VDSASLIILSDYFMAVNIVVKNTAPAPDGKTKGAQALSMRISGNFAAFYNCKFYGFQDTI 207

Query: 220 CDDRGRHFFKDCYIEGTVDFIFGNGKSLYLKTVINSVAEGTGVITAQARENSTDESGFTF 279
           CDD G HFFKDCY+EGT DFIFG+G S+YL T ++ V +G  VI A A +++ ++SG++F
Sbjct: 208 CDDTGNHFFKDCYVEGTFDFIFGSGTSMYLGTQLHVVGDGIRVIAAHAGKSAEEKSGYSF 267

Query: 280 AYCNITGT-GNTYLGRAWKERTRVVFAYTYMGTLINTQGWSDKMQGTEPRKSMYYGEYKC 339
            +C +TGT G  YLGRAW    +VV+AYT M +++N  GW +        K+++YGEYKC
Sbjct: 268 VHCKVTGTGGGIYLGRAWMSHPKVVYAYTEMTSVVNPTGWQENKTPAHD-KTVFYGEYKC 327

Query: 340 KGPGADSTGRAHYTRILTDDEAKAFLSMTYIHGNKWVLPPPRL 382
            GPG+    R  +T+ + D EA  FLS+ YI G+KW+LPPP L
Sbjct: 328 SGPGSHKAKRVPFTQDIDDKEANCFLSLGYIQGSKWLLPPPAL 361

BLAST of CmaCh10G007900 vs. ExPASy Swiss-Prot
Match: Q84WM7 (Pectinesterase PPME1 OS=Arabidopsis thaliana OX=3702 GN=PPME1 PE=1 SV=1)

HSP 1 Score: 348.6 bits (893), Expect = 9.2e-95
Identity = 171/359 (47.63%), Postives = 240/359 (66.85%), Query Frame = 0

Query: 24  ITMLIPAAIVVEARTKPIPAEPSQLDEWIAHNMREYRQRKAKETGAKALDRRLAKAEDCA 83
           + M+    +V      PIP    Q+ +W   N+    QR       K LD  L  AE   
Sbjct: 12  LLMVFVTPMVFADDVTPIPEGKPQVAQWFNANVGPLAQR-------KGLDPALVAAEAAP 71

Query: 84  RLITVKQDGQGNFSTITQAIESIPSGNSERVVVWIGGGTYREKITIDSSKPFVTLYGQTG 143
           R+I V   G G F T+T AI+S+P+GN++RV++ +  G Y+EK+TID +KPF+TL GQ  
Sbjct: 72  RIINVNPKG-GEFKTLTDAIKSVPAGNTKRVIIKMAPGEYKEKVTIDRNKPFITLMGQPN 131

Query: 144 KMPVISYDGTASEFGTVESATVAVESDYFVAVNVAFVNSAPMPDAGRTGGQAVAMRISGD 203
            MPVI+YDGTA+++GTV+SA++ + SDYF+AVN+   N+AP PD    G QA++MRISG+
Sbjct: 132 AMPVITYDGTAAKYGTVDSASLIILSDYFMAVNIVVKNTAPAPDGKTKGAQALSMRISGN 191

Query: 204 KAAFHHCQFIGFQDTLCDDRGRHFFKDCYIEGTVDFIFGNGKSLYLKTVINSVAEGTGVI 263
            AAF++C+F GFQDT+CDD G HFFKDCY+EGT DFIFG+G S+YL T ++ V +G  VI
Sbjct: 192 FAAFYNCKFYGFQDTICDDTGNHFFKDCYVEGTFDFIFGSGTSMYLGTQLHVVGDGIRVI 251

Query: 264 TAQARENSTDESGFTFAYCNITGT-GNTYLGRAWKERTRVVFAYTYMGTLINTQGWSDKM 323
            A A +++ ++SG++F +C +TGT G  YLGRAW    +VV+AYT M +++N  GW +  
Sbjct: 252 AAHAGKSAEEKSGYSFVHCKVTGTGGGIYLGRAWMSHPKVVYAYTEMTSVVNPTGWQENK 311

Query: 324 QGTEPRKSMYYGEYKCKGPGADSTGRAHYTRILTDDEAKAFLSMTYIHGNKWVLPPPRL 382
                 K+++YGEYKC GPG+    R  +T+ + D EA  FLS+ YI G+KW+LPPP L
Sbjct: 312 TPAHD-KTVFYGEYKCSGPGSHKAKRVPFTQDIDDKEANRFLSLGYIQGSKWLLPPPAL 361

BLAST of CmaCh10G007900 vs. ExPASy Swiss-Prot
Match: Q9LY18 (Probable pectinesterase 49 OS=Arabidopsis thaliana OX=3702 GN=PME49 PE=2 SV=1)

HSP 1 Score: 336.7 bits (862), Expect = 3.6e-91
Identity = 167/363 (46.01%), Postives = 243/363 (66.94%), Query Frame = 0

Query: 18  LAVVQLITMLIPAAIVVEARTKPIPAEPSQLDEWIAHNMREYRQRKAKETGAKALDRRLA 77
           LA+V L+       ++ +  T PIPA+ +Q+ +W   N++ + QR+        LD  L 
Sbjct: 6   LALVALLVFFASPVVLADDIT-PIPADRAQIPQWFMANVKPFSQRRG------TLDPELE 65

Query: 78  KAEDCARLITVKQDGQGNFSTITQAIESIPSGNSERVVVWIGGGTYREKITIDSSKPFVT 137
            AE   R+I V Q+G G+F TI  AI+SIP  N  RV++ +  G Y EK+T+D  +P+VT
Sbjct: 66  AAEASRRVIIVNQNGGGDFKTINAAIKSIPLANKNRVIIKLAPGIYHEKVTVDVGRPYVT 125

Query: 138 LYGQTGKMPVISYDGTASEFGTVESATVAVESDYFVAVNVAFVNSAPMPDAGRTGGQAVA 197
           L G+ G    ++Y GTA+++GTVESAT+ V +  F+A N+  +N++PMP  G T GQA+A
Sbjct: 126 LLGKPGAETNLTYAGTAAKYGTVESATLIVWATNFLAANLNIINTSPMPKPG-TQGQALA 185

Query: 198 MRISGDKAAFHHCQFIGFQDTLCDDRGRHFFKDCYIEGTVDFIFGNGKSLYLKTVINSVA 257
           MRI+GDKAAF++C+F GFQDTLCDDRG HFFK+CYIEGT DFIFG G SLYL T +++V 
Sbjct: 186 MRINGDKAAFYNCRFYGFQDTLCDDRGNHFFKNCYIEGTYDFIFGRGASLYLTTQLHAVG 245

Query: 258 EGTGVITAQARENSTDESGFTFAYCNITGTG-NTYLGRAWKERTRVVFAYTYMGTLINTQ 317
           +G  VI A  R+++T+++G++F +C +TG G   YLGRAW    +VV++YT M +++N  
Sbjct: 246 DGLRVIAAHNRQSTTEQNGYSFVHCKVTGVGTGIYLGRAWMSHPKVVYSYTEMSSVVNPS 305

Query: 318 GWSDKMQGTEPRKSMYYGEYKCKGPGADSTGRAHYTRILTDDEAKAFLSMTYIHGNKWVL 377
           GW +        K+++YGEY C GPG+    R  +T+ + + EA  FL++ YI G+KW+L
Sbjct: 306 GWQENRVRAHD-KTVFYGEYMCTGPGSHKAKRVAHTQDIDNKEASQFLTLGYIKGSKWLL 359

Query: 378 PPP 380
           PPP
Sbjct: 366 PPP 359

BLAST of CmaCh10G007900 vs. ExPASy TrEMBL
Match: A0A6J1JI92 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111484834 PE=4 SV=1)

HSP 1 Score: 786.2 bits (2029), Expect = 6.4e-224
Identity = 392/392 (100.00%), Postives = 392/392 (100.00%), Query Frame = 0

Query: 1   MSRPAVASSFPATVALVLAVVQLITMLIPAAIVVEARTKPIPAEPSQLDEWIAHNMREYR 60
           MSRPAVASSFPATVALVLAVVQLITMLIPAAIVVEARTKPIPAEPSQLDEWIAHNMREYR
Sbjct: 1   MSRPAVASSFPATVALVLAVVQLITMLIPAAIVVEARTKPIPAEPSQLDEWIAHNMREYR 60

Query: 61  QRKAKETGAKALDRRLAKAEDCARLITVKQDGQGNFSTITQAIESIPSGNSERVVVWIGG 120
           QRKAKETGAKALDRRLAKAEDCARLITVKQDGQGNFSTITQAIESIPSGNSERVVVWIGG
Sbjct: 61  QRKAKETGAKALDRRLAKAEDCARLITVKQDGQGNFSTITQAIESIPSGNSERVVVWIGG 120

Query: 121 GTYREKITIDSSKPFVTLYGQTGKMPVISYDGTASEFGTVESATVAVESDYFVAVNVAFV 180
           GTYREKITIDSSKPFVTLYGQTGKMPVISYDGTASEFGTVESATVAVESDYFVAVNVAFV
Sbjct: 121 GTYREKITIDSSKPFVTLYGQTGKMPVISYDGTASEFGTVESATVAVESDYFVAVNVAFV 180

Query: 181 NSAPMPDAGRTGGQAVAMRISGDKAAFHHCQFIGFQDTLCDDRGRHFFKDCYIEGTVDFI 240
           NSAPMPDAGRTGGQAVAMRISGDKAAFHHCQFIGFQDTLCDDRGRHFFKDCYIEGTVDFI
Sbjct: 181 NSAPMPDAGRTGGQAVAMRISGDKAAFHHCQFIGFQDTLCDDRGRHFFKDCYIEGTVDFI 240

Query: 241 FGNGKSLYLKTVINSVAEGTGVITAQARENSTDESGFTFAYCNITGTGNTYLGRAWKERT 300
           FGNGKSLYLKTVINSVAEGTGVITAQARENSTDESGFTFAYCNITGTGNTYLGRAWKERT
Sbjct: 241 FGNGKSLYLKTVINSVAEGTGVITAQARENSTDESGFTFAYCNITGTGNTYLGRAWKERT 300

Query: 301 RVVFAYTYMGTLINTQGWSDKMQGTEPRKSMYYGEYKCKGPGADSTGRAHYTRILTDDEA 360
           RVVFAYTYMGTLINTQGWSDKMQGTEPRKSMYYGEYKCKGPGADSTGRAHYTRILTDDEA
Sbjct: 301 RVVFAYTYMGTLINTQGWSDKMQGTEPRKSMYYGEYKCKGPGADSTGRAHYTRILTDDEA 360

Query: 361 KAFLSMTYIHGNKWVLPPPRLSLLPVSSQCHG 393
           KAFLSMTYIHGNKWVLPPPRLSLLPVSSQCHG
Sbjct: 361 KAFLSMTYIHGNKWVLPPPRLSLLPVSSQCHG 392

BLAST of CmaCh10G007900 vs. ExPASy TrEMBL
Match: A0A6J1HB45 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111461773 PE=4 SV=1)

HSP 1 Score: 770.8 bits (1989), Expect = 2.8e-219
Identity = 384/392 (97.96%), Postives = 386/392 (98.47%), Query Frame = 0

Query: 1   MSRPAVASSFPATVALVLAVVQLITMLIPAAIVVEARTKPIPAEPSQLDEWIAHNMREYR 60
           MSRPAVASSFPATVALVLAVVQLIT+LIPAAIVVEARTK IPAEPSQLDEWIAHNMREYR
Sbjct: 1   MSRPAVASSFPATVALVLAVVQLITILIPAAIVVEARTKTIPAEPSQLDEWIAHNMREYR 60

Query: 61  QRKAKETGAKALDRRLAKAEDCARLITVKQDGQGNFSTITQAIESIPSGNSERVVVWIGG 120
           QRKA ETGAK LD RLAKAEDCARLITVKQDGQGNFSTITQAIESIPSGN ERVVVWIGG
Sbjct: 61  QRKANETGAKTLDPRLAKAEDCARLITVKQDGQGNFSTITQAIESIPSGNRERVVVWIGG 120

Query: 121 GTYREKITIDSSKPFVTLYGQTGKMPVISYDGTASEFGTVESATVAVESDYFVAVNVAFV 180
           GTYREKITIDSSKPFVTLYGQTGKMPVISYDGTASEFGT ESATVAVESDYFVAVNVAFV
Sbjct: 121 GTYREKITIDSSKPFVTLYGQTGKMPVISYDGTASEFGTAESATVAVESDYFVAVNVAFV 180

Query: 181 NSAPMPDAGRTGGQAVAMRISGDKAAFHHCQFIGFQDTLCDDRGRHFFKDCYIEGTVDFI 240
           NSAPMPDAGRTGGQAVAMRISGDKAAFHHCQFIGFQDTLCDDRGRHFFKDCYIEGTVDFI
Sbjct: 181 NSAPMPDAGRTGGQAVAMRISGDKAAFHHCQFIGFQDTLCDDRGRHFFKDCYIEGTVDFI 240

Query: 241 FGNGKSLYLKTVINSVAEGTGVITAQARENSTDESGFTFAYCNITGTGNTYLGRAWKERT 300
           FGNGKSLYLKTVINSVAEGTGVITAQARENSTD+SGFTFAYCNITGTGNTYLGRAWKERT
Sbjct: 241 FGNGKSLYLKTVINSVAEGTGVITAQARENSTDDSGFTFAYCNITGTGNTYLGRAWKERT 300

Query: 301 RVVFAYTYMGTLINTQGWSDKMQGTEPRKSMYYGEYKCKGPGADSTGRAHYTRILTDDEA 360
           RVVFAYTYMGTLINTQGWSDKMQGTEPRKSMYYGEYKCKGPGADSTGRAHYTRILTDDEA
Sbjct: 301 RVVFAYTYMGTLINTQGWSDKMQGTEPRKSMYYGEYKCKGPGADSTGRAHYTRILTDDEA 360

Query: 361 KAFLSMTYIHGNKWVLPPPRLSLLPVSSQCHG 393
           KAFLSMTYIHGNKWVLPPPRLSLLPVSSQCHG
Sbjct: 361 KAFLSMTYIHGNKWVLPPPRLSLLPVSSQCHG 392

BLAST of CmaCh10G007900 vs. ExPASy TrEMBL
Match: A0A1S3BRI5 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103492397 PE=4 SV=1)

HSP 1 Score: 646.7 bits (1667), Expect = 6.1e-182
Identity = 321/387 (82.95%), Postives = 350/387 (90.44%), Query Frame = 0

Query: 1   MSRPAVASSFPATVALVLAVV-QLITML-IPAAIVVEARTKPIPAEPSQLDEWIAHNMRE 60
           M RP VASSF ATVAL LAV+ QLI ++ IP+ ++VEARTK IPA+ S+LDEWIAHNMRE
Sbjct: 1   MFRPTVASSFSATVALALAVMFQLIILITIPSTVLVEARTKTIPADSSKLDEWIAHNMRE 60

Query: 61  YRQRKAKETGAKALDRRLAKAEDCARLITVKQDGQGNFSTITQAIESIPSGNSERVVVWI 120
           Y  RK  ETG KALDRRLA+AEDC RLITV++DG+GNFSTIT+AIESIPSGN  RVVVWI
Sbjct: 61  YNDRKMNETGIKALDRRLAEAEDCVRLITVRKDGRGNFSTITEAIESIPSGNRRRVVVWI 120

Query: 121 GGGTYREKITIDSSKPFVTLYGQTGKMPVISYDGTASEFGTVESATVAVESDYFVAVNVA 180
           GGG YREKITID+SKPFVTLYGQ GK PVI++DGTASEFGTV+SATVAVESDYFVAVN+ 
Sbjct: 121 GGGVYREKITIDASKPFVTLYGQKGKRPVITFDGTASEFGTVKSATVAVESDYFVAVNLT 180

Query: 181 FVNSAPMPDAGRTGGQAVAMRISGDKAAFHHCQFIGFQDTLCDDRGRHFFKDCYIEGTVD 240
           FVNSAPMP+ G TGGQAVAMRISGDKAAFH C FIGFQDTLCDDRGRHFF+DCYI+GTVD
Sbjct: 181 FVNSAPMPELGGTGGQAVAMRISGDKAAFHECHFIGFQDTLCDDRGRHFFRDCYIQGTVD 240

Query: 241 FIFGNGKSLYLKTVINSVAEGTGVITAQARENSTDESGFTFAYCNITGTGNTYLGRAWKE 300
           FIFGNGKSLYLKT INSVAEG GVITAQARE++TDESGFTFAYCNITGTGNTYLGRAWKE
Sbjct: 241 FIFGNGKSLYLKTTINSVAEGIGVITAQAREDATDESGFTFAYCNITGTGNTYLGRAWKE 300

Query: 301 RTRVVFAYTYMGTLINTQGWSDKMQGTEPRKSMYYGEYKCKGPGADSTGRAHYTRILTDD 360
           RTRVVFAYTYMGTLINT+GWSDKM G++PRKSMYYGEYKCKGPGA S+GR  Y RIL+D 
Sbjct: 301 RTRVVFAYTYMGTLINTEGWSDKMHGSQPRKSMYYGEYKCKGPGATSSGRVKYARILSDV 360

Query: 361 EAKAFLSMTYIHGNKWVLPPPRLSLLP 386
           EAKAFLSMTYIHGNKW+LPPP LS  P
Sbjct: 361 EAKAFLSMTYIHGNKWLLPPPDLSFSP 387

BLAST of CmaCh10G007900 vs. ExPASy TrEMBL
Match: A0A0A0M299 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_1G597780 PE=4 SV=1)

HSP 1 Score: 639.4 bits (1648), Expect = 9.7e-180
Identity = 312/387 (80.62%), Postives = 347/387 (89.66%), Query Frame = 0

Query: 1   MSRPAVASSFPATVALVLAVV--QLITMLIPAAIVVEARTKPIPAEPSQLDEWIAHNMRE 60
           M RP VASSFPATVA  + V+   +I ++IP+ +VVEA TK IPA+ S+LDEWI HNM+E
Sbjct: 1   MFRPTVASSFPATVAFAMGVMLQLIILIIIPSTVVVEAHTKTIPADSSKLDEWIGHNMKE 60

Query: 61  YRQRKAKETGAKALDRRLAKAEDCARLITVKQDGQGNFSTITQAIESIPSGNSERVVVWI 120
           Y  RK  ETG KALDRRLA+AEDC +LITV++DG+GNFSTIT+AI+SIPSGN  RVVVWI
Sbjct: 61  YNDRKTNETGIKALDRRLAEAEDCVQLITVRKDGRGNFSTITEAIDSIPSGNRRRVVVWI 120

Query: 121 GGGTYREKITIDSSKPFVTLYGQTGKMPVISYDGTASEFGTVESATVAVESDYFVAVNVA 180
           GGG YREKITID+SKPFVTLYGQ GK P+I++DGTASEFGTV+SATVAVESDYFVAVN+ 
Sbjct: 121 GGGVYREKITIDASKPFVTLYGQKGKRPMITFDGTASEFGTVKSATVAVESDYFVAVNLT 180

Query: 181 FVNSAPMPDAGRTGGQAVAMRISGDKAAFHHCQFIGFQDTLCDDRGRHFFKDCYIEGTVD 240
           FVNSAPMP+ G TGGQAVAMRISGDKAAFH C FIGFQDTLCDDRGRHFFKDCY++GTVD
Sbjct: 181 FVNSAPMPELGGTGGQAVAMRISGDKAAFHGCHFIGFQDTLCDDRGRHFFKDCYVQGTVD 240

Query: 241 FIFGNGKSLYLKTVINSVAEGTGVITAQARENSTDESGFTFAYCNITGTGNTYLGRAWKE 300
           FIFGNGKSLYLKT INSVAEGTGVITAQARE++TDESGFTFAYCNITGTG+TYLGRAWKE
Sbjct: 241 FIFGNGKSLYLKTTINSVAEGTGVITAQAREDATDESGFTFAYCNITGTGDTYLGRAWKE 300

Query: 301 RTRVVFAYTYMGTLINTQGWSDKMQGTEPRKSMYYGEYKCKGPGADSTGRAHYTRILTDD 360
           RTRVVFAYTYMGTLINT+GWSDKM G++PRKSMYYGEYKCKGPGA  +GR  Y RIL+D 
Sbjct: 301 RTRVVFAYTYMGTLINTEGWSDKMHGSQPRKSMYYGEYKCKGPGATPSGRVKYARILSDV 360

Query: 361 EAKAFLSMTYIHGNKWVLPPPRLSLLP 386
           EAKAFLSMTYIHGNKW+LPPP LSL P
Sbjct: 361 EAKAFLSMTYIHGNKWLLPPPDLSLSP 387

BLAST of CmaCh10G007900 vs. ExPASy TrEMBL
Match: A0A6J1D0K0 (Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111016481 PE=4 SV=1)

HSP 1 Score: 625.5 bits (1612), Expect = 1.4e-175
Identity = 309/382 (80.89%), Postives = 341/382 (89.27%), Query Frame = 0

Query: 1   MSRPAVASSFPATVALVLAVVQLITMLIPAAIVVEARTKPIPAEPSQLDEWIAHNMREYR 60
           M RPAVASS P  VA+ LAV+QLI +++PAA+V  ARTKPIPAEPS+LDEWI +NM+EY 
Sbjct: 1   MYRPAVASSSPVAVAIALAVIQLI-IILPAAVV--ARTKPIPAEPSKLDEWIENNMKEYH 60

Query: 61  QRKAKETGAKALDRRLAKAEDCARLITVKQDGQGNFSTITQAIESIPSGNSERVVVWIGG 120
           +RKA +TG KALD+RLA AED  RLITV+ DG+GNF+TIT AIESIP+GN+ RV+VWI G
Sbjct: 61  ERKANDTGVKALDKRLAMAEDAVRLITVRPDGRGNFTTITAAIESIPAGNTARVIVWIAG 120

Query: 121 GTYREKITIDSSKPFVTLYGQTGKMPVISYDGTASEFGTVESATVAVESDYFVAVNVAFV 180
           G YREKITID+SKPFVTLYGQ GKMPVI++DGTASEFGTV+SATVAVESDYFVAVN+AFV
Sbjct: 121 GIYREKITIDASKPFVTLYGQNGKMPVITFDGTASEFGTVQSATVAVESDYFVAVNIAFV 180

Query: 181 NSAPMPDAGRTGGQAVAMRISGDKAAFHHCQFIGFQDTLCDDRGRHFFKDCYIEGTVDFI 240
           NSA MPD   +GGQAVAMRISGDKAAFHHC FIGFQDTLCDDRGRHFFKDCYIEGTVDFI
Sbjct: 181 NSAAMPDVRVSGGQAVAMRISGDKAAFHHCHFIGFQDTLCDDRGRHFFKDCYIEGTVDFI 240

Query: 241 FGNGKSLYLKTVINSVAEGTGVITAQARENSTDESGFTFAYCNITGTGNTYLGRAWKERT 300
           FGNGKSLYLKT INSVAEG GVITAQARE++TD+SGFTF YCNITGTGNTYLGRAWKERT
Sbjct: 241 FGNGKSLYLKTRINSVAEGMGVITAQAREDATDDSGFTFVYCNITGTGNTYLGRAWKERT 300

Query: 301 RVVFAYTYMGTLINTQGWSDKMQGTEPRKSMYYGEYKCKGPGADSTGRAHYTRILTDDEA 360
           RVVFAYTYMG LINT+GWSDKM   EPRKSMYYGEYKC GPGA   GR  Y RIL+D+EA
Sbjct: 301 RVVFAYTYMGNLINTEGWSDKMHHGEPRKSMYYGEYKCMGPGATPAGRVKYARILSDEEA 360

Query: 361 KAFLSMTYIHGNKWVLPPPRLS 383
           +AFLSMTYIHGNKW+LPPP LS
Sbjct: 361 RAFLSMTYIHGNKWLLPPPLLS 379

BLAST of CmaCh10G007900 vs. NCBI nr
Match: XP_022987214.1 (putative pectinesterase 63 [Cucurbita maxima])

HSP 1 Score: 786.2 bits (2029), Expect = 1.3e-223
Identity = 392/392 (100.00%), Postives = 392/392 (100.00%), Query Frame = 0

Query: 1   MSRPAVASSFPATVALVLAVVQLITMLIPAAIVVEARTKPIPAEPSQLDEWIAHNMREYR 60
           MSRPAVASSFPATVALVLAVVQLITMLIPAAIVVEARTKPIPAEPSQLDEWIAHNMREYR
Sbjct: 1   MSRPAVASSFPATVALVLAVVQLITMLIPAAIVVEARTKPIPAEPSQLDEWIAHNMREYR 60

Query: 61  QRKAKETGAKALDRRLAKAEDCARLITVKQDGQGNFSTITQAIESIPSGNSERVVVWIGG 120
           QRKAKETGAKALDRRLAKAEDCARLITVKQDGQGNFSTITQAIESIPSGNSERVVVWIGG
Sbjct: 61  QRKAKETGAKALDRRLAKAEDCARLITVKQDGQGNFSTITQAIESIPSGNSERVVVWIGG 120

Query: 121 GTYREKITIDSSKPFVTLYGQTGKMPVISYDGTASEFGTVESATVAVESDYFVAVNVAFV 180
           GTYREKITIDSSKPFVTLYGQTGKMPVISYDGTASEFGTVESATVAVESDYFVAVNVAFV
Sbjct: 121 GTYREKITIDSSKPFVTLYGQTGKMPVISYDGTASEFGTVESATVAVESDYFVAVNVAFV 180

Query: 181 NSAPMPDAGRTGGQAVAMRISGDKAAFHHCQFIGFQDTLCDDRGRHFFKDCYIEGTVDFI 240
           NSAPMPDAGRTGGQAVAMRISGDKAAFHHCQFIGFQDTLCDDRGRHFFKDCYIEGTVDFI
Sbjct: 181 NSAPMPDAGRTGGQAVAMRISGDKAAFHHCQFIGFQDTLCDDRGRHFFKDCYIEGTVDFI 240

Query: 241 FGNGKSLYLKTVINSVAEGTGVITAQARENSTDESGFTFAYCNITGTGNTYLGRAWKERT 300
           FGNGKSLYLKTVINSVAEGTGVITAQARENSTDESGFTFAYCNITGTGNTYLGRAWKERT
Sbjct: 241 FGNGKSLYLKTVINSVAEGTGVITAQARENSTDESGFTFAYCNITGTGNTYLGRAWKERT 300

Query: 301 RVVFAYTYMGTLINTQGWSDKMQGTEPRKSMYYGEYKCKGPGADSTGRAHYTRILTDDEA 360
           RVVFAYTYMGTLINTQGWSDKMQGTEPRKSMYYGEYKCKGPGADSTGRAHYTRILTDDEA
Sbjct: 301 RVVFAYTYMGTLINTQGWSDKMQGTEPRKSMYYGEYKCKGPGADSTGRAHYTRILTDDEA 360

Query: 361 KAFLSMTYIHGNKWVLPPPRLSLLPVSSQCHG 393
           KAFLSMTYIHGNKWVLPPPRLSLLPVSSQCHG
Sbjct: 361 KAFLSMTYIHGNKWVLPPPRLSLLPVSSQCHG 392

BLAST of CmaCh10G007900 vs. NCBI nr
Match: XP_022961183.1 (putative pectinesterase 63 [Cucurbita moschata] >KAG7023751.1 putative pectinesterase 50 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 770.8 bits (1989), Expect = 5.8e-219
Identity = 384/392 (97.96%), Postives = 386/392 (98.47%), Query Frame = 0

Query: 1   MSRPAVASSFPATVALVLAVVQLITMLIPAAIVVEARTKPIPAEPSQLDEWIAHNMREYR 60
           MSRPAVASSFPATVALVLAVVQLIT+LIPAAIVVEARTK IPAEPSQLDEWIAHNMREYR
Sbjct: 1   MSRPAVASSFPATVALVLAVVQLITILIPAAIVVEARTKTIPAEPSQLDEWIAHNMREYR 60

Query: 61  QRKAKETGAKALDRRLAKAEDCARLITVKQDGQGNFSTITQAIESIPSGNSERVVVWIGG 120
           QRKA ETGAK LD RLAKAEDCARLITVKQDGQGNFSTITQAIESIPSGN ERVVVWIGG
Sbjct: 61  QRKANETGAKTLDPRLAKAEDCARLITVKQDGQGNFSTITQAIESIPSGNRERVVVWIGG 120

Query: 121 GTYREKITIDSSKPFVTLYGQTGKMPVISYDGTASEFGTVESATVAVESDYFVAVNVAFV 180
           GTYREKITIDSSKPFVTLYGQTGKMPVISYDGTASEFGT ESATVAVESDYFVAVNVAFV
Sbjct: 121 GTYREKITIDSSKPFVTLYGQTGKMPVISYDGTASEFGTAESATVAVESDYFVAVNVAFV 180

Query: 181 NSAPMPDAGRTGGQAVAMRISGDKAAFHHCQFIGFQDTLCDDRGRHFFKDCYIEGTVDFI 240
           NSAPMPDAGRTGGQAVAMRISGDKAAFHHCQFIGFQDTLCDDRGRHFFKDCYIEGTVDFI
Sbjct: 181 NSAPMPDAGRTGGQAVAMRISGDKAAFHHCQFIGFQDTLCDDRGRHFFKDCYIEGTVDFI 240

Query: 241 FGNGKSLYLKTVINSVAEGTGVITAQARENSTDESGFTFAYCNITGTGNTYLGRAWKERT 300
           FGNGKSLYLKTVINSVAEGTGVITAQARENSTD+SGFTFAYCNITGTGNTYLGRAWKERT
Sbjct: 241 FGNGKSLYLKTVINSVAEGTGVITAQARENSTDDSGFTFAYCNITGTGNTYLGRAWKERT 300

Query: 301 RVVFAYTYMGTLINTQGWSDKMQGTEPRKSMYYGEYKCKGPGADSTGRAHYTRILTDDEA 360
           RVVFAYTYMGTLINTQGWSDKMQGTEPRKSMYYGEYKCKGPGADSTGRAHYTRILTDDEA
Sbjct: 301 RVVFAYTYMGTLINTQGWSDKMQGTEPRKSMYYGEYKCKGPGADSTGRAHYTRILTDDEA 360

Query: 361 KAFLSMTYIHGNKWVLPPPRLSLLPVSSQCHG 393
           KAFLSMTYIHGNKWVLPPPRLSLLPVSSQCHG
Sbjct: 361 KAFLSMTYIHGNKWVLPPPRLSLLPVSSQCHG 392

BLAST of CmaCh10G007900 vs. NCBI nr
Match: XP_023515642.1 (putative pectinesterase 63 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 766.5 bits (1978), Expect = 1.1e-217
Identity = 382/392 (97.45%), Postives = 385/392 (98.21%), Query Frame = 0

Query: 1   MSRPAVASSFPATVALVLAVVQLITMLIPAAIVVEARTKPIPAEPSQLDEWIAHNMREYR 60
           MSRPAVASSFPATVALVLAVVQLIT+LIPAAIVVEARTK IPAEPSQLDEWIAHNMREYR
Sbjct: 1   MSRPAVASSFPATVALVLAVVQLITILIPAAIVVEARTKTIPAEPSQLDEWIAHNMREYR 60

Query: 61  QRKAKETGAKALDRRLAKAEDCARLITVKQDGQGNFSTITQAIESIPSGNSERVVVWIGG 120
           QRKA ETGAK LD RLAKAEDCAR+ITVKQDGQGNFSTITQAIESIPSGN ERVVVWIGG
Sbjct: 61  QRKANETGAKTLDPRLAKAEDCARIITVKQDGQGNFSTITQAIESIPSGNRERVVVWIGG 120

Query: 121 GTYREKITIDSSKPFVTLYGQTGKMPVISYDGTASEFGTVESATVAVESDYFVAVNVAFV 180
           GTYREKITIDSSKPFVTLYGQTGKMPVISYDGTASEFGT ESATVAVESDYFVAVNVAFV
Sbjct: 121 GTYREKITIDSSKPFVTLYGQTGKMPVISYDGTASEFGTAESATVAVESDYFVAVNVAFV 180

Query: 181 NSAPMPDAGRTGGQAVAMRISGDKAAFHHCQFIGFQDTLCDDRGRHFFKDCYIEGTVDFI 240
           NSAPMPDAGRTGGQAVAMRISGDKAAFHHCQFIGFQDTLCDDRGRHFFKDCYIEGTVDFI
Sbjct: 181 NSAPMPDAGRTGGQAVAMRISGDKAAFHHCQFIGFQDTLCDDRGRHFFKDCYIEGTVDFI 240

Query: 241 FGNGKSLYLKTVINSVAEGTGVITAQARENSTDESGFTFAYCNITGTGNTYLGRAWKERT 300
           FGNGKSLYLKTVINSVAEGTGVITAQARENSTD+SGFTFAYCNITGTGNTYLGRAWKERT
Sbjct: 241 FGNGKSLYLKTVINSVAEGTGVITAQARENSTDDSGFTFAYCNITGTGNTYLGRAWKERT 300

Query: 301 RVVFAYTYMGTLINTQGWSDKMQGTEPRKSMYYGEYKCKGPGADSTGRAHYTRILTDDEA 360
           RVVFAYTYMGTLINTQGWSDKMQGTEPRKSMYYGEYKCKGPGADSTGRAHYTRILTD EA
Sbjct: 301 RVVFAYTYMGTLINTQGWSDKMQGTEPRKSMYYGEYKCKGPGADSTGRAHYTRILTDYEA 360

Query: 361 KAFLSMTYIHGNKWVLPPPRLSLLPVSSQCHG 393
           KAFLSMTYIHGNKWVLPPPRLSLLPVSSQCHG
Sbjct: 361 KAFLSMTYIHGNKWVLPPPRLSLLPVSSQCHG 392

BLAST of CmaCh10G007900 vs. NCBI nr
Match: KAG6590056.1 (putative pectinesterase 50, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 766.1 bits (1977), Expect = 1.4e-217
Identity = 381/392 (97.19%), Postives = 385/392 (98.21%), Query Frame = 0

Query: 1   MSRPAVASSFPATVALVLAVVQLITMLIPAAIVVEARTKPIPAEPSQLDEWIAHNMREYR 60
           M+RPAVASSFPATVALVLAVVQLIT+LIPAAIVVEARTK IPAEPSQLDEWIAHNMREYR
Sbjct: 1   MARPAVASSFPATVALVLAVVQLITILIPAAIVVEARTKTIPAEPSQLDEWIAHNMREYR 60

Query: 61  QRKAKETGAKALDRRLAKAEDCARLITVKQDGQGNFSTITQAIESIPSGNSERVVVWIGG 120
           QRKA ETGAK LD RLAKAEDCARLITVKQDGQGNFSTITQAI+SIPSGN ERVVVWIGG
Sbjct: 61  QRKANETGAKTLDPRLAKAEDCARLITVKQDGQGNFSTITQAIDSIPSGNRERVVVWIGG 120

Query: 121 GTYREKITIDSSKPFVTLYGQTGKMPVISYDGTASEFGTVESATVAVESDYFVAVNVAFV 180
           GTYREKITIDSSKPFVTLYGQ GKMPVISYDGTASEFGT ESATVAVESDYFVAVNVAFV
Sbjct: 121 GTYREKITIDSSKPFVTLYGQIGKMPVISYDGTASEFGTAESATVAVESDYFVAVNVAFV 180

Query: 181 NSAPMPDAGRTGGQAVAMRISGDKAAFHHCQFIGFQDTLCDDRGRHFFKDCYIEGTVDFI 240
           NSAPMPDAGRTGGQAVAMRISGDKAAFHHCQFIGFQDTLCDDRGRHFFKDCYIEGTVDFI
Sbjct: 181 NSAPMPDAGRTGGQAVAMRISGDKAAFHHCQFIGFQDTLCDDRGRHFFKDCYIEGTVDFI 240

Query: 241 FGNGKSLYLKTVINSVAEGTGVITAQARENSTDESGFTFAYCNITGTGNTYLGRAWKERT 300
           FGNGKSLYLKTVINSVAEGTGVITAQARENSTD+SGFTFAYCNITGTGNTYLGRAWKERT
Sbjct: 241 FGNGKSLYLKTVINSVAEGTGVITAQARENSTDDSGFTFAYCNITGTGNTYLGRAWKERT 300

Query: 301 RVVFAYTYMGTLINTQGWSDKMQGTEPRKSMYYGEYKCKGPGADSTGRAHYTRILTDDEA 360
           RVVFAYTYMGTLINTQGWSDKMQGTEPRKSMYYGEYKCKGPGADSTGRAHYTRILTDDEA
Sbjct: 301 RVVFAYTYMGTLINTQGWSDKMQGTEPRKSMYYGEYKCKGPGADSTGRAHYTRILTDDEA 360

Query: 361 KAFLSMTYIHGNKWVLPPPRLSLLPVSSQCHG 393
           KAFLSMTYIHGNKWVLPPPRLSLLPVSSQCHG
Sbjct: 361 KAFLSMTYIHGNKWVLPPPRLSLLPVSSQCHG 392

BLAST of CmaCh10G007900 vs. NCBI nr
Match: XP_008450966.1 (PREDICTED: putative pectinesterase 63 [Cucumis melo])

HSP 1 Score: 646.7 bits (1667), Expect = 1.3e-181
Identity = 321/387 (82.95%), Postives = 350/387 (90.44%), Query Frame = 0

Query: 1   MSRPAVASSFPATVALVLAVV-QLITML-IPAAIVVEARTKPIPAEPSQLDEWIAHNMRE 60
           M RP VASSF ATVAL LAV+ QLI ++ IP+ ++VEARTK IPA+ S+LDEWIAHNMRE
Sbjct: 1   MFRPTVASSFSATVALALAVMFQLIILITIPSTVLVEARTKTIPADSSKLDEWIAHNMRE 60

Query: 61  YRQRKAKETGAKALDRRLAKAEDCARLITVKQDGQGNFSTITQAIESIPSGNSERVVVWI 120
           Y  RK  ETG KALDRRLA+AEDC RLITV++DG+GNFSTIT+AIESIPSGN  RVVVWI
Sbjct: 61  YNDRKMNETGIKALDRRLAEAEDCVRLITVRKDGRGNFSTITEAIESIPSGNRRRVVVWI 120

Query: 121 GGGTYREKITIDSSKPFVTLYGQTGKMPVISYDGTASEFGTVESATVAVESDYFVAVNVA 180
           GGG YREKITID+SKPFVTLYGQ GK PVI++DGTASEFGTV+SATVAVESDYFVAVN+ 
Sbjct: 121 GGGVYREKITIDASKPFVTLYGQKGKRPVITFDGTASEFGTVKSATVAVESDYFVAVNLT 180

Query: 181 FVNSAPMPDAGRTGGQAVAMRISGDKAAFHHCQFIGFQDTLCDDRGRHFFKDCYIEGTVD 240
           FVNSAPMP+ G TGGQAVAMRISGDKAAFH C FIGFQDTLCDDRGRHFF+DCYI+GTVD
Sbjct: 181 FVNSAPMPELGGTGGQAVAMRISGDKAAFHECHFIGFQDTLCDDRGRHFFRDCYIQGTVD 240

Query: 241 FIFGNGKSLYLKTVINSVAEGTGVITAQARENSTDESGFTFAYCNITGTGNTYLGRAWKE 300
           FIFGNGKSLYLKT INSVAEG GVITAQARE++TDESGFTFAYCNITGTGNTYLGRAWKE
Sbjct: 241 FIFGNGKSLYLKTTINSVAEGIGVITAQAREDATDESGFTFAYCNITGTGNTYLGRAWKE 300

Query: 301 RTRVVFAYTYMGTLINTQGWSDKMQGTEPRKSMYYGEYKCKGPGADSTGRAHYTRILTDD 360
           RTRVVFAYTYMGTLINT+GWSDKM G++PRKSMYYGEYKCKGPGA S+GR  Y RIL+D 
Sbjct: 301 RTRVVFAYTYMGTLINTEGWSDKMHGSQPRKSMYYGEYKCKGPGATSSGRVKYARILSDV 360

Query: 361 EAKAFLSMTYIHGNKWVLPPPRLSLLP 386
           EAKAFLSMTYIHGNKW+LPPP LS  P
Sbjct: 361 EAKAFLSMTYIHGNKWLLPPPDLSFSP 387

BLAST of CmaCh10G007900 vs. TAIR 10
Match: AT5G61680.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 354.4 bits (908), Expect = 1.2e-97
Identity = 171/364 (46.98%), Postives = 243/364 (66.76%), Query Frame = 0

Query: 17  VLAVVQLITMLIPAAIVVEARTKPIPAEPSQLDEWIAHNMREYRQRKAKETGAKALDRRL 76
           V  +V ++ ++I + +V      PIP    ++++W   N                     
Sbjct: 6   VSLIVTILLVVITSPVVFGNDAAPIPENKGRIEQWFNTN--------------------- 65

Query: 77  AKAEDCARLITVKQDGQGNFSTITQAIESIPSGNSERVVVWIGGGTYREKITIDSSKPFV 136
                      VKQ+G+G+F TIT+AI S+ +GN+ RV++ IG G Y+EK+TID SKPF+
Sbjct: 66  -----------VKQNGRGHFKTITEAINSVRAGNTRRVIIKIGPGVYKEKVTIDRSKPFI 125

Query: 137 TLYGQTGKMPVISYDGTASEFGTVESATVAVESDYFVAVNVAFVNSAPMPDAGRTGGQAV 196
           TLYG    MPV+++DGTA+++GTV+SAT+ V SDYF+AVN+   NSAPMPD  R G QA+
Sbjct: 126 TLYGHPNAMPVLTFDGTAAQYGTVDSATLIVLSDYFMAVNIILKNSAPMPDGKRKGAQAL 185

Query: 197 AMRISGDKAAFHHCQFIGFQDTLCDDRGRHFFKDCYIEGTVDFIFGNGKSLYLKTVINSV 256
           +MRISG+KAAF++C+F G+QDT+CDD G HFFKDCYIEGT DFIFG+G+SLYL T +N V
Sbjct: 186 SMRISGNKAAFYNCKFYGYQDTICDDTGNHFFKDCYIEGTFDFIFGSGRSLYLGTQLNVV 245

Query: 257 AEGTGVITAQARENSTDESGFTFAYCNITGTG-NTYLGRAWKERTRVVFAYTYMGTLINT 316
            +G  VITA A +++ ++SG++F +C +TGTG   YLGR+W    +VV+AYT M +++N 
Sbjct: 246 GDGIRVITAHAGKSAAEKSGYSFVHCKVTGTGTGIYLGRSWMSHPKVVYAYTDMSSVVNP 305

Query: 317 QGWSDKMQGTEPRKSMYYGEYKCKGPGADSTGRAHYTRILTDDEAKAFLSMTYIHGNKWV 376
            GW +  +     K+++YGEYKC G G+    R  YT+ + D EAK F+S+ YI G+ W+
Sbjct: 306 SGWQENREAGRD-KTVFYGEYKCTGTGSHKEKRVKYTQDIDDIEAKYFISLGYIQGSSWL 336

Query: 377 LPPP 380
           LPPP
Sbjct: 366 LPPP 336

BLAST of CmaCh10G007900 vs. TAIR 10
Match: AT5G07430.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 352.4 bits (903), Expect = 4.6e-97
Identity = 171/357 (47.90%), Postives = 247/357 (69.19%), Query Frame = 0

Query: 26  MLIPAAIVVEARTKPIPAEPSQLDEWIAHNMREYRQRKAKETGAKALDRRLAKAEDCARL 85
           ++  + +V+   T PIP   +Q+ +W   N++ Y QRK        LD  L  AE   ++
Sbjct: 13  VVFASPVVLATDTDPIPENRAQIPQWFKTNVKPYSQRKG------TLDPALEAAEAARQI 72

Query: 86  ITVKQDGQGNFSTITQAIESIPSGNSERVVVWIGGGTYREKITIDSSKPFVTLYGQTGKM 145
           ITV Q G  NF T+ +AI+SIP+GN  RV++ +  G Y EK+TID ++PF+TL GQ G  
Sbjct: 73  ITVNQKGGANFKTLNEAIKSIPTGNKNRVIIKLAPGVYNEKVTIDIARPFITLLGQPGAE 132

Query: 146 PVISYDGTASEFGTVESATVAVESDYFVAVNVAFVNSAPMPDAGRTGGQAVAMRISGDKA 205
            V++Y GTA+++GTVESAT+ V ++YF A ++   N+APMP  G + GQA+AMRI+ DKA
Sbjct: 133 TVLTYHGTAAQYGTVESATLIVWAEYFQAAHLTIKNTAPMPKPG-SQGQALAMRINADKA 192

Query: 206 AFHHCQFIGFQDTLCDDRGRHFFKDCYIEGTVDFIFGNGKSLYLKTVINSVAEGTGVITA 265
           AF+ C+F GFQDTLCDD+G HFFKDCYIEGT DFIFG G SLYL T +++V +G  VITA
Sbjct: 193 AFYSCRFHGFQDTLCDDKGNHFFKDCYIEGTYDFIFGRGASLYLNTQLHAVGDGLRVITA 252

Query: 266 QARENSTDESGFTFAYCNITGTG-NTYLGRAWKERTRVVFAYTYMGTLINTQGWSDKM-Q 325
           Q R+++T+++G+TF +C +TGTG   YLGR+W    +VV+A+T M +++N  GW + + +
Sbjct: 253 QGRQSATEQNGYTFVHCKVTGTGTGIYLGRSWMSHPKVVYAFTEMTSVVNPSGWRENLNR 312

Query: 326 GTEPRKSMYYGEYKCKGPGADSTGRAHYTRILTDDEAKAFLSMTYIHGNKWVLPPPR 381
           G +  K+++YGEYKC GPG+    R  YT+ +  +E   FL++ YI G+ W+LPPP+
Sbjct: 313 GYD--KTVFYGEYKCFGPGSHLEKRVPYTQDIDKNEVTPFLTLGYIKGSTWLLPPPK 360

BLAST of CmaCh10G007900 vs. TAIR 10
Match: AT5G07410.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 349.7 bits (896), Expect = 2.9e-96
Identity = 169/343 (49.27%), Postives = 235/343 (68.51%), Query Frame = 0

Query: 40  PIPAEPSQLDEWIAHNMREYRQRKAKETGAKALDRRLAKAEDCARLITVKQDGQGNFSTI 99
           PIP    Q+ +W   ++    QR       K LD  L  AE   R+I V   G G F T+
Sbjct: 28  PIPEGKPQVVQWFNTHVGPLAQR-------KGLDPALVAAEAAPRIINVNPKG-GEFKTL 87

Query: 100 TQAIESIPSGNSERVVVWIGGGTYREKITIDSSKPFVTLYGQTGKMPVISYDGTASEFGT 159
           T AI+S+P+GN++RV++ +  G YREK+TID +KPF+TL GQ   MPVI+YDGTA+++GT
Sbjct: 88  TDAIKSVPAGNTKRVIIKMAHGEYREKVTIDRNKPFITLMGQPNAMPVITYDGTAAKYGT 147

Query: 160 VESATVAVESDYFVAVNVAFVNSAPMPDAGRTGGQAVAMRISGDKAAFHHCQFIGFQDTL 219
           V+SA++ + SDYF+AVN+   N+AP PD    G QA++MRISG+ AAF++C+F GFQDT+
Sbjct: 148 VDSASLIILSDYFMAVNIVVKNTAPAPDGKTKGAQALSMRISGNFAAFYNCKFYGFQDTI 207

Query: 220 CDDRGRHFFKDCYIEGTVDFIFGNGKSLYLKTVINSVAEGTGVITAQARENSTDESGFTF 279
           CDD G HFFKDCY+EGT DFIFG+G S+YL T ++ V +G  VI A A +++ ++SG++F
Sbjct: 208 CDDTGNHFFKDCYVEGTFDFIFGSGTSMYLGTQLHVVGDGIRVIAAHAGKSAEEKSGYSF 267

Query: 280 AYCNITGT-GNTYLGRAWKERTRVVFAYTYMGTLINTQGWSDKMQGTEPRKSMYYGEYKC 339
            +C +TGT G  YLGRAW    +VV+AYT M +++N  GW +        K+++YGEYKC
Sbjct: 268 VHCKVTGTGGGIYLGRAWMSHPKVVYAYTEMTSVVNPTGWQENKTPAHD-KTVFYGEYKC 327

Query: 340 KGPGADSTGRAHYTRILTDDEAKAFLSMTYIHGNKWVLPPPRL 382
            GPG+    R  +T+ + D EA  FLS+ YI G+KW+LPPP L
Sbjct: 328 SGPGSHKAKRVPFTQDIDDKEANCFLSLGYIQGSKWLLPPPAL 361

BLAST of CmaCh10G007900 vs. TAIR 10
Match: AT1G69940.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 348.6 bits (893), Expect = 6.6e-96
Identity = 171/359 (47.63%), Postives = 240/359 (66.85%), Query Frame = 0

Query: 24  ITMLIPAAIVVEARTKPIPAEPSQLDEWIAHNMREYRQRKAKETGAKALDRRLAKAEDCA 83
           + M+    +V      PIP    Q+ +W   N+    QR       K LD  L  AE   
Sbjct: 12  LLMVFVTPMVFADDVTPIPEGKPQVAQWFNANVGPLAQR-------KGLDPALVAAEAAP 71

Query: 84  RLITVKQDGQGNFSTITQAIESIPSGNSERVVVWIGGGTYREKITIDSSKPFVTLYGQTG 143
           R+I V   G G F T+T AI+S+P+GN++RV++ +  G Y+EK+TID +KPF+TL GQ  
Sbjct: 72  RIINVNPKG-GEFKTLTDAIKSVPAGNTKRVIIKMAPGEYKEKVTIDRNKPFITLMGQPN 131

Query: 144 KMPVISYDGTASEFGTVESATVAVESDYFVAVNVAFVNSAPMPDAGRTGGQAVAMRISGD 203
            MPVI+YDGTA+++GTV+SA++ + SDYF+AVN+   N+AP PD    G QA++MRISG+
Sbjct: 132 AMPVITYDGTAAKYGTVDSASLIILSDYFMAVNIVVKNTAPAPDGKTKGAQALSMRISGN 191

Query: 204 KAAFHHCQFIGFQDTLCDDRGRHFFKDCYIEGTVDFIFGNGKSLYLKTVINSVAEGTGVI 263
            AAF++C+F GFQDT+CDD G HFFKDCY+EGT DFIFG+G S+YL T ++ V +G  VI
Sbjct: 192 FAAFYNCKFYGFQDTICDDTGNHFFKDCYVEGTFDFIFGSGTSMYLGTQLHVVGDGIRVI 251

Query: 264 TAQARENSTDESGFTFAYCNITGT-GNTYLGRAWKERTRVVFAYTYMGTLINTQGWSDKM 323
            A A +++ ++SG++F +C +TGT G  YLGRAW    +VV+AYT M +++N  GW +  
Sbjct: 252 AAHAGKSAEEKSGYSFVHCKVTGTGGGIYLGRAWMSHPKVVYAYTEMTSVVNPTGWQENK 311

Query: 324 QGTEPRKSMYYGEYKCKGPGADSTGRAHYTRILTDDEAKAFLSMTYIHGNKWVLPPPRL 382
                 K+++YGEYKC GPG+    R  +T+ + D EA  FLS+ YI G+KW+LPPP L
Sbjct: 312 TPAHD-KTVFYGEYKCSGPGSHKAKRVPFTQDIDDKEANRFLSLGYIQGSKWLLPPPAL 361

BLAST of CmaCh10G007900 vs. TAIR 10
Match: AT5G07420.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 336.7 bits (862), Expect = 2.6e-92
Identity = 167/363 (46.01%), Postives = 243/363 (66.94%), Query Frame = 0

Query: 18  LAVVQLITMLIPAAIVVEARTKPIPAEPSQLDEWIAHNMREYRQRKAKETGAKALDRRLA 77
           LA+V L+       ++ +  T PIPA+ +Q+ +W   N++ + QR+        LD  L 
Sbjct: 6   LALVALLVFFASPVVLADDIT-PIPADRAQIPQWFMANVKPFSQRRG------TLDPELE 65

Query: 78  KAEDCARLITVKQDGQGNFSTITQAIESIPSGNSERVVVWIGGGTYREKITIDSSKPFVT 137
            AE   R+I V Q+G G+F TI  AI+SIP  N  RV++ +  G Y EK+T+D  +P+VT
Sbjct: 66  AAEASRRVIIVNQNGGGDFKTINAAIKSIPLANKNRVIIKLAPGIYHEKVTVDVGRPYVT 125

Query: 138 LYGQTGKMPVISYDGTASEFGTVESATVAVESDYFVAVNVAFVNSAPMPDAGRTGGQAVA 197
           L G+ G    ++Y GTA+++GTVESAT+ V +  F+A N+  +N++PMP  G T GQA+A
Sbjct: 126 LLGKPGAETNLTYAGTAAKYGTVESATLIVWATNFLAANLNIINTSPMPKPG-TQGQALA 185

Query: 198 MRISGDKAAFHHCQFIGFQDTLCDDRGRHFFKDCYIEGTVDFIFGNGKSLYLKTVINSVA 257
           MRI+GDKAAF++C+F GFQDTLCDDRG HFFK+CYIEGT DFIFG G SLYL T +++V 
Sbjct: 186 MRINGDKAAFYNCRFYGFQDTLCDDRGNHFFKNCYIEGTYDFIFGRGASLYLTTQLHAVG 245

Query: 258 EGTGVITAQARENSTDESGFTFAYCNITGTG-NTYLGRAWKERTRVVFAYTYMGTLINTQ 317
           +G  VI A  R+++T+++G++F +C +TG G   YLGRAW    +VV++YT M +++N  
Sbjct: 246 DGLRVIAAHNRQSTTEQNGYSFVHCKVTGVGTGIYLGRAWMSHPKVVYSYTEMSSVVNPS 305

Query: 318 GWSDKMQGTEPRKSMYYGEYKCKGPGADSTGRAHYTRILTDDEAKAFLSMTYIHGNKWVL 377
           GW +        K+++YGEY C GPG+    R  +T+ + + EA  FL++ YI G+KW+L
Sbjct: 306 GWQENRVRAHD-KTVFYGEYMCTGPGSHKAKRVAHTQDIDNKEASQFLTLGYIKGSKWLL 359

Query: 378 PPP 380
           PPP
Sbjct: 366 PPP 359

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9FKF31.7e-9646.98Putative pectinesterase 63 OS=Arabidopsis thaliana OX=3702 GN=PME63 PE=3 SV=2[more]
Q9LY176.4e-9647.90Probable pectinesterase 50 OS=Arabidopsis thaliana OX=3702 GN=PME50 PE=2 SV=1[more]
Q9LY194.2e-9549.27Probable pectinesterase 48 OS=Arabidopsis thaliana OX=3702 GN=PME48 PE=2 SV=2[more]
Q84WM79.2e-9547.63Pectinesterase PPME1 OS=Arabidopsis thaliana OX=3702 GN=PPME1 PE=1 SV=1[more]
Q9LY183.6e-9146.01Probable pectinesterase 49 OS=Arabidopsis thaliana OX=3702 GN=PME49 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1JI926.4e-224100.00Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111484834 PE=4 SV=1[more]
A0A6J1HB452.8e-21997.96Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111461773 PE=4 SV=1[more]
A0A1S3BRI56.1e-18282.95Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103492397 PE=4 SV=1[more]
A0A0A0M2999.7e-18080.62Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_1G597780 PE=4 SV=1[more]
A0A6J1D0K01.4e-17580.89Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111016481 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
XP_022987214.11.3e-223100.00putative pectinesterase 63 [Cucurbita maxima][more]
XP_022961183.15.8e-21997.96putative pectinesterase 63 [Cucurbita moschata] >KAG7023751.1 putative pectinest... [more]
XP_023515642.11.1e-21797.45putative pectinesterase 63 [Cucurbita pepo subsp. pepo][more]
KAG6590056.11.4e-21797.19putative pectinesterase 50, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_008450966.11.3e-18182.95PREDICTED: putative pectinesterase 63 [Cucumis melo][more]
Match NameE-valueIdentityDescription
AT5G61680.11.2e-9746.98Pectin lyase-like superfamily protein [more]
AT5G07430.14.6e-9747.90Pectin lyase-like superfamily protein [more]
AT5G07410.12.9e-9649.27Pectin lyase-like superfamily protein [more]
AT1G69940.16.6e-9647.63Pectin lyase-like superfamily protein [more]
AT5G07420.12.6e-9246.01Pectin lyase-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR012334Pectin lyase foldGENE3D2.160.20.10coord: 80..377
e-value: 4.3E-102
score: 343.7
IPR000070Pectinesterase, catalyticPFAMPF01095Pectinesterasecoord: 86..372
e-value: 1.7E-67
score: 227.3
NoneNo IPR availablePANTHERPTHR31321ACYL-COA THIOESTER HYDROLASE YBHC-RELATEDcoord: 19..379
NoneNo IPR availablePANTHERPTHR31321:SF30PECTINESTERASEcoord: 19..379
IPR033131Pectinesterase, Asp active sitePROSITEPS00503PECTINESTERASE_2coord: 233..242
IPR011050Pectin lyase fold/virulence factorSUPERFAMILY51126Pectin lyase-likecoord: 83..375

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh10G007900.1CmaCh10G007900.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042545 cell wall modification
biological_process GO:0045490 pectin catabolic process
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0045330 aspartyl esterase activity
molecular_function GO:0030599 pectinesterase activity