CmaCh10G006080 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh10G006080
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
DescriptionRegulator of Vps4 activity in the MVB pathway protein
LocationCma_Chr10: 2812531 .. 2815649 (-)
RNA-Seq ExpressionCmaCh10G006080
SyntenyCmaCh10G006080
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTTGGATGGGATTCTCGGCCGAGGCTTTACATCCAAGTGGTATTAATTCCGATCTATTTTTTTTCTTTTTCATTTTTGAGTTTCTTGAGAGCTTAAACAAGGGATGTTTTTGTTTTCTTGATATTTGTAGCAAATCCCAGATTAAATTGACCAAAAGTCGGATCGATGTGATTCGGAGGAAGAAGAAAGCAACTCTGAAATTTCTGAAGAAAGATATTGCTGATCTACTCGCAAATGGGCTGGATATTAATGCTTATGGAAGGGTATTCTTTTTTTCTTGTCACACAATTGTTTCCTTCGTTTGAATTGTAAAATGTTTTGATTGTCCTAGGGTGTAAGCTCAAATAAGTAGTTTATAAAGGCCATGATTTCTTTTTTGGATTAATATTCTGTTTATGTGGTGTCGGTGTTTGTTCTTGCCTGAAAACCATATCTATGTGGTGTTTGATTAAACTTTGTTGTTGATTCTCTGATTATTATGCCGACTTCTATGGTATCTGTCTAGTAATTGGCTCAAACAAACTGCTGCTTTTGCTTGTATGGTGTGAAACAGGCTGAGGGGCTTCTAGTTGAATTAACAATTTCGTCCTGTTATGATTTCGTGGAGCAATCATGTGATACCGTGTTGAAGCATCTTCCAATCATGCAAAAGCAGAGGTATTGAATCATGCTTGTGTTCTGATTACTTATTAGATTTCCACTCGGATAAGCTCATGTAACAGCCTAAGTCCACTGCTAGATATTATCTTTTTTGGGTTTTCTCTTCTAGGCTTCCCCTCAAAATTTTAAAACGTGTCGGAGAGATCGTTCCTTTCTCTAATTGATGTGGGATCTCATAGCTTACTAGCAGGGAGGATATCTTTATATCATTCCAAAGCATGTAGATAACTTCATGTTGTCTTACACTTGTCAATTATATAGTTTCTGGCTAAGAGCATGTCGATAACTTCATGTCGTCTTACACTGGTCGATTATATAGTTGCTGGCTAAGAACGATTATATCATTTTACACAAATTTTGTTTGTGACTTTATACTTGATAATTTATGTTCGTCTGATTCCCTCTAGCTGCCATGTTTACAGGGAATGTCCAGAGGAATGTTGTGAGGCTATTGCATCTCTAATGTTTGCTGCTGCTAGATTTTCGGATTTGCCAGAATTGCGCGAGCTCAGGCAAATCTTTCAGGAGAGGTTTGGGACTTCTGTGGAACATTTGGAGAACCGGAAGGTAATACCCAAGTAACTAGCTTCAGGTTTCCTGGATCTTTCTTAATGAGAGACTAATCACTAATCTATTTCTGTAGTTTGTTGAGAATTTAGCTTCCAAGCCTTCCACATTGGAGAAGAAAGTTCAGTTATTACAAGACATAGCTTTGGAATTTTCAATTAAATGGGATTCTGTGGGCTTTGAGAAGAGAATGTCCACTCCCCCAGCCTATGCTCAGGTGCACCTCGTTCCCCTATTTTACTACTTTCCAACGTGCAAGAAACTGAGTTGAATCCTTAAAAGTATCAATTTTTCAACTTTCCCAACGTCTGAACTTCCCATGTCCTTCTTCTGCAGGGCTTACCTAAAGATTTCGGGGCTCACAATGCTGCAATAGAAAAAAGTCTTTCCCATGCTAAGGAAATTGATCCAAGAGTGAAGGATGATGGTGTGTCATTCAAAGAAAACTTTGAGCAACCAAATGCCAGGCACAGATTCCTCAATCCTAGTGATAGTACCATTGCAGGAGGAAAAGAACTCAAGTTTCAGCCGAAACAAGAATTTCCCCGACACAGACATGAGAATAGAGTATACGACGAAGAAGAGACGATCTTGAAATCCGATGGTCGTATAAATCTTTATGGAGAGAAAAAAGGTTCTACAGTTCCCAAGCATGAAGCTAGAATTGGTAGAATGGGCAGTTCTTCTTCGAGTGAAGTACTGAAAGATGCTGATAATGGACTAGTTGTGCAGAATGGCAGAGAACGGAGAGTTCCAGATTACTTGAAGTCTTCACCTTACAACAATTCAGGTCTTGCTCCAACTAGTAAGCATGAAGCTGGAAATGGTATGATGAGCTCTGCTTTCGGACCTCGTAGAATGGGCAGTTCTTCTTCCAGTGAAGTATTGGGAGATGTAGATGATGGAACAACAGTTGTGCATAATGACCGAGAGAGGACAGTTCCAAATTACTTAAAATCTCCACCTTACAGTAAGTCAGGTCTTGCTCCTAGTGACATTAAAGAGCCATCATCTGGCAATACTCATACGGGGCATGGATATGCAGTTCATCAAGGGAAGGCTGAGGAGGATAAACATCAAAATACAAAGCCAAGCTTCAACAGTATCCTCCCTCCTCCATATGTAAAGGCCAACTCTAGAAGGAAGGATAACAAGGATAGGAGCCATGACAACAATTGTGTCTCAATGGGTCCTCAGAAGCCTGTCAAGTCAGAAATGATCACCGGTGTCGAATTAGAACCCCGCCATTCTGACCATGAGAGGCAGGTTATTAGTCCTTTGAGAGCAAGTAGTCATGGTGGTGAAATGGATCGTTTCTTTGGTGGTCGGATACCTCCCGACGCTCTGCCGAAACCAAGATCAGTTCGGAGAAGGCACCACAAGCCACGTTCCAGTCATTCAGTTGATGACAATGCTGAGGACATTCGAACGGTGAGAAAGAAGTCAAGAAGTAGTAGTAGGAGAAGGGATGACAAACGAGGCTTGCAGCTGTTAATAGACGAACAGGAGAATGAAAGGGATGAAGAAGAAAGGATAATAGACAAGCTCTTGATACATTATAGTAAGAAACCATCAACCTTTGAGCCAGGAAAGGTAAGAAGGAAGCCTAAAAATCATCTGTCTCTTGAAAATGGTACTGATTCTGCCAAGTCCCCTTCAAATCTAAGAAGCAGAGATGAAGCTGATAAGCAGGCAGACACAGTTCATCCACCAGCTAGATCAGCTTCCCTACCTCCTGAACATCATCTGGGTCCATCAGAAACAACCAAAGTATATACTCGAGCTATTTCCTTTCAACCAGACCGATCAAGTGCAGCTAAGCATGTTCATCCTAAATTACCCGACTACGATGATTTGGCTGCCCGATTTGCAGCCTTGAGAGGAAGGTAA

mRNA sequence

ATGTTGGATGGGATTCTCGGCCGAGGCTTTACATCCAACAAATCCCAGATTAAATTGACCAAAAGTCGGATCGATGTGATTCGGAGGAAGAAGAAAGCAACTCTGAAATTTCTGAAGAAAGATATTGCTGATCTACTCGCAAATGGGCTGGATATTAATGCTTATGGAAGGGCTGAGGGGCTTCTAGTTGAATTAACAATTTCGTCCTGTTATGATTTCGTGGAGCAATCATGTGATACCGTGTTGAAGCATCTTCCAATCATGCAAAAGCAGAGGGAATGTCCAGAGGAATGTTGTGAGGCTATTGCATCTCTAATGTTTGCTGCTGCTAGATTTTCGGATTTGCCAGAATTGCGCGAGCTCAGGCAAATCTTTCAGGAGAGGTTTGGGACTTCTGTGGAACATTTGGAGAACCGGAAGTTTGTTGAGAATTTAGCTTCCAAGCCTTCCACATTGGAGAAGAAAGTTCAGTTATTACAAGACATAGCTTTGGAATTTTCAATTAAATGGGATTCTGTGGGCTTTGAGAAGAGAATGTCCACTCCCCCAGCCTATGCTCAGGGCTTACCTAAAGATTTCGGGGCTCACAATGCTGCAATAGAAAAAAGTCTTTCCCATGCTAAGGAAATTGATCCAAGAGTGAAGGATGATGGTGTGTCATTCAAAGAAAACTTTGAGCAACCAAATGCCAGGCACAGATTCCTCAATCCTAGTGATAGTACCATTGCAGGAGGAAAAGAACTCAAGTTTCAGCCGAAACAAGAATTTCCCCGACACAGACATGAGAATAGAGTATACGACGAAGAAGAGACGATCTTGAAATCCGATGGTCGTATAAATCTTTATGGAGAGAAAAAAGGTTCTACAGTTCCCAAGCATGAAGCTAGAATTGGTAGAATGGGCAGTTCTTCTTCGAGTGAAGTACTGAAAGATGCTGATAATGGACTAGTTGTGCAGAATGGCAGAGAACGGAGAGTTCCAGATTACTTGAAGTCTTCACCTTACAACAATTCAGGTCTTGCTCCAACTAGTAAGCATGAAGCTGGAAATGGTATGATGAGCTCTGCTTTCGGACCTCGTAGAATGGGCAGTTCTTCTTCCAGTGAAGTATTGGGAGATGTAGATGATGGAACAACAGTTGTGCATAATGACCGAGAGAGGACAGTTCCAAATTACTTAAAATCTCCACCTTACAGTAAGTCAGGTCTTGCTCCTAGTGACATTAAAGAGCCATCATCTGGCAATACTCATACGGGGCATGGATATGCAGTTCATCAAGGGAAGGCTGAGGAGGATAAACATCAAAATACAAAGCCAAGCTTCAACAGTATCCTCCCTCCTCCATATGTAAAGGCCAACTCTAGAAGGAAGGATAACAAGGATAGGAGCCATGACAACAATTGTGTCTCAATGGGTCCTCAGAAGCCTGTCAAGTCAGAAATGATCACCGGTGTCGAATTAGAACCCCGCCATTCTGACCATGAGAGGCAGGTTATTAGTCCTTTGAGAGCAAGTAGTCATGGTGGTGAAATGGATCGTTTCTTTGGTGGTCGGATACCTCCCGACGCTCTGCCGAAACCAAGATCAGTTCGGAGAAGGCACCACAAGCCACGTTCCAGTCATTCAGTTGATGACAATGCTGAGGACATTCGAACGGTGAGAAAGAAGTCAAGAAGTAGTAGTAGGAGAAGGGATGACAAACGAGGCTTGCAGCTGTTAATAGACGAACAGGAGAATGAAAGGGATGAAGAAGAAAGGATAATAGACAAGCTCTTGATACATTATAGTAAGAAACCATCAACCTTTGAGCCAGGAAAGGTAAGAAGGAAGCCTAAAAATCATCTGTCTCTTGAAAATGGTACTGATTCTGCCAAGTCCCCTTCAAATCTAAGAAGCAGAGATGAAGCTGATAAGCAGGCAGACACAGTTCATCCACCAGCTAGATCAGCTTCCCTACCTCCTGAACATCATCTGGGTCCATCAGAAACAACCAAAGTATATACTCGAGCTATTTCCTTTCAACCAGACCGATCAAGTGCAGCTAAGCATGTTCATCCTAAATTACCCGACTACGATGATTTGGCTGCCCGATTTGCAGCCTTGAGAGGAAGGTAA

Coding sequence (CDS)

ATGTTGGATGGGATTCTCGGCCGAGGCTTTACATCCAACAAATCCCAGATTAAATTGACCAAAAGTCGGATCGATGTGATTCGGAGGAAGAAGAAAGCAACTCTGAAATTTCTGAAGAAAGATATTGCTGATCTACTCGCAAATGGGCTGGATATTAATGCTTATGGAAGGGCTGAGGGGCTTCTAGTTGAATTAACAATTTCGTCCTGTTATGATTTCGTGGAGCAATCATGTGATACCGTGTTGAAGCATCTTCCAATCATGCAAAAGCAGAGGGAATGTCCAGAGGAATGTTGTGAGGCTATTGCATCTCTAATGTTTGCTGCTGCTAGATTTTCGGATTTGCCAGAATTGCGCGAGCTCAGGCAAATCTTTCAGGAGAGGTTTGGGACTTCTGTGGAACATTTGGAGAACCGGAAGTTTGTTGAGAATTTAGCTTCCAAGCCTTCCACATTGGAGAAGAAAGTTCAGTTATTACAAGACATAGCTTTGGAATTTTCAATTAAATGGGATTCTGTGGGCTTTGAGAAGAGAATGTCCACTCCCCCAGCCTATGCTCAGGGCTTACCTAAAGATTTCGGGGCTCACAATGCTGCAATAGAAAAAAGTCTTTCCCATGCTAAGGAAATTGATCCAAGAGTGAAGGATGATGGTGTGTCATTCAAAGAAAACTTTGAGCAACCAAATGCCAGGCACAGATTCCTCAATCCTAGTGATAGTACCATTGCAGGAGGAAAAGAACTCAAGTTTCAGCCGAAACAAGAATTTCCCCGACACAGACATGAGAATAGAGTATACGACGAAGAAGAGACGATCTTGAAATCCGATGGTCGTATAAATCTTTATGGAGAGAAAAAAGGTTCTACAGTTCCCAAGCATGAAGCTAGAATTGGTAGAATGGGCAGTTCTTCTTCGAGTGAAGTACTGAAAGATGCTGATAATGGACTAGTTGTGCAGAATGGCAGAGAACGGAGAGTTCCAGATTACTTGAAGTCTTCACCTTACAACAATTCAGGTCTTGCTCCAACTAGTAAGCATGAAGCTGGAAATGGTATGATGAGCTCTGCTTTCGGACCTCGTAGAATGGGCAGTTCTTCTTCCAGTGAAGTATTGGGAGATGTAGATGATGGAACAACAGTTGTGCATAATGACCGAGAGAGGACAGTTCCAAATTACTTAAAATCTCCACCTTACAGTAAGTCAGGTCTTGCTCCTAGTGACATTAAAGAGCCATCATCTGGCAATACTCATACGGGGCATGGATATGCAGTTCATCAAGGGAAGGCTGAGGAGGATAAACATCAAAATACAAAGCCAAGCTTCAACAGTATCCTCCCTCCTCCATATGTAAAGGCCAACTCTAGAAGGAAGGATAACAAGGATAGGAGCCATGACAACAATTGTGTCTCAATGGGTCCTCAGAAGCCTGTCAAGTCAGAAATGATCACCGGTGTCGAATTAGAACCCCGCCATTCTGACCATGAGAGGCAGGTTATTAGTCCTTTGAGAGCAAGTAGTCATGGTGGTGAAATGGATCGTTTCTTTGGTGGTCGGATACCTCCCGACGCTCTGCCGAAACCAAGATCAGTTCGGAGAAGGCACCACAAGCCACGTTCCAGTCATTCAGTTGATGACAATGCTGAGGACATTCGAACGGTGAGAAAGAAGTCAAGAAGTAGTAGTAGGAGAAGGGATGACAAACGAGGCTTGCAGCTGTTAATAGACGAACAGGAGAATGAAAGGGATGAAGAAGAAAGGATAATAGACAAGCTCTTGATACATTATAGTAAGAAACCATCAACCTTTGAGCCAGGAAAGGTAAGAAGGAAGCCTAAAAATCATCTGTCTCTTGAAAATGGTACTGATTCTGCCAAGTCCCCTTCAAATCTAAGAAGCAGAGATGAAGCTGATAAGCAGGCAGACACAGTTCATCCACCAGCTAGATCAGCTTCCCTACCTCCTGAACATCATCTGGGTCCATCAGAAACAACCAAAGTATATACTCGAGCTATTTCCTTTCAACCAGACCGATCAAGTGCAGCTAAGCATGTTCATCCTAAATTACCCGACTACGATGATTTGGCTGCCCGATTTGCAGCCTTGAGAGGAAGGTAA

Protein sequence

MLDGILGRGFTSNKSQIKLTKSRIDVIRRKKKATLKFLKKDIADLLANGLDINAYGRAEGLLVELTISSCYDFVEQSCDTVLKHLPIMQKQRECPEECCEAIASLMFAAARFSDLPELRELRQIFQERFGTSVEHLENRKFVENLASKPSTLEKKVQLLQDIALEFSIKWDSVGFEKRMSTPPAYAQGLPKDFGAHNAAIEKSLSHAKEIDPRVKDDGVSFKENFEQPNARHRFLNPSDSTIAGGKELKFQPKQEFPRHRHENRVYDEEETILKSDGRINLYGEKKGSTVPKHEARIGRMGSSSSSEVLKDADNGLVVQNGRERRVPDYLKSSPYNNSGLAPTSKHEAGNGMMSSAFGPRRMGSSSSSEVLGDVDDGTTVVHNDRERTVPNYLKSPPYSKSGLAPSDIKEPSSGNTHTGHGYAVHQGKAEEDKHQNTKPSFNSILPPPYVKANSRRKDNKDRSHDNNCVSMGPQKPVKSEMITGVELEPRHSDHERQVISPLRASSHGGEMDRFFGGRIPPDALPKPRSVRRRHHKPRSSHSVDDNAEDIRTVRKKSRSSSRRRDDKRGLQLLIDEQENERDEEERIIDKLLIHYSKKPSTFEPGKVRRKPKNHLSLENGTDSAKSPSNLRSRDEADKQADTVHPPARSASLPPEHHLGPSETTKVYTRAISFQPDRSSAAKHVHPKLPDYDDLAARFAALRGR
Homology
BLAST of CmaCh10G006080 vs. ExPASy Swiss-Prot
Match: Q54I39 (IST1-like protein OS=Dictyostelium discoideum OX=44689 GN=DDB_G0289029 PE=3 SV=1)

HSP 1 Score: 62.4 bits (150), Expect = 2.4e-08
Identity = 41/165 (24.85%), Postives = 84/165 (50.91%), Query Frame = 0

Query: 14  KSQIKLTKSRIDVIRRKKKATLKFLKKDIADLLANGLDINAYGRAEGLLVELTISSCYDF 73
           K Q+KL  SRI +++ KK   ++  K+++A+LL    + +A  R E ++ +  +  C+  
Sbjct: 14  KVQLKLAVSRIQILKNKKANIVRDEKRNVAELLRKKNEESARIRVETIIRDEYLIECFQI 73

Query: 74  VEQSCDTVLKHLPIMQKQRECPEECCEAIASLMFAAARFSDLPELRELRQIFQERFGTSV 133
           +E  C+ +   + ++    E P E  E+I +L++++ R   +PEL +++   + ++G  +
Sbjct: 74  IEVLCELLHARINLINATTEMPLEMKESIFTLVYSSQRI-QIPELEQIKNQLKAKYGKGL 133

Query: 134 EHLE--------NRKFVENLASKPSTLEKKVQLLQDIALEFSIKW 171
           E+          N K V  L+          Q L +IA +F++ W
Sbjct: 134 ENEANCHCSTHVNPKIVHKLSYATPDPSIIFQTLSEIAEKFNVDW 177

BLAST of CmaCh10G006080 vs. ExPASy Swiss-Prot
Match: P53990 (IST1 homolog OS=Homo sapiens OX=9606 GN=IST1 PE=1 SV=1)

HSP 1 Score: 55.1 bits (131), Expect = 3.8e-06
Identity = 34/118 (28.81%), Postives = 66/118 (55.93%), Query Frame = 0

Query: 5   ILGRGFTSNKSQI--KLTKSRIDVIRRKKKATLKFLKKDIADLLANGLDINAYGRAEGLL 64
           +LG GF + + ++  +L  +R+ ++ +KK    +  +K+IAD LA G D  A  R E ++
Sbjct: 1   MLGSGFKAERLRVNLRLVINRLKLLEKKKTELAQKARKEIADYLAAGKDERARIRVEHII 60

Query: 65  VELTISSCYDFVEQSCDTVLKHLPIMQKQRECPEECCEAIASLMFAAARF-SDLPELR 120
            E  +    + +E  CD +L    ++Q  +E      E++++L++AA R  S++ EL+
Sbjct: 61  REDYLVEAMEILELYCDLLLARFGLIQSMKELDSGLAESVSTLIWAAPRLQSEVAELK 118

BLAST of CmaCh10G006080 vs. ExPASy Swiss-Prot
Match: Q9CX00 (IST1 homolog OS=Mus musculus OX=10090 GN=Ist1 PE=1 SV=1)

HSP 1 Score: 55.1 bits (131), Expect = 3.8e-06
Identity = 34/118 (28.81%), Postives = 66/118 (55.93%), Query Frame = 0

Query: 5   ILGRGFTSNKSQI--KLTKSRIDVIRRKKKATLKFLKKDIADLLANGLDINAYGRAEGLL 64
           +LG GF + + ++  +L  +R+ ++ +KK    +  +K+IAD LA G D  A  R E ++
Sbjct: 1   MLGSGFKAERLRVNLRLVINRLKLLEKKKTELAQKARKEIADYLAAGKDERARIRVEHII 60

Query: 65  VELTISSCYDFVEQSCDTVLKHLPIMQKQRECPEECCEAIASLMFAAARF-SDLPELR 120
            E  +    + +E  CD +L    ++Q  +E      E++++L++AA R  S++ EL+
Sbjct: 61  REDYLVEAMEILELYCDLLLARFGLIQSMKELDSGLAESVSTLIWAAPRLQSEVAELK 118

BLAST of CmaCh10G006080 vs. ExPASy Swiss-Prot
Match: Q5R6G8 (IST1 homolog OS=Pongo abelii OX=9601 GN=IST1 PE=2 SV=1)

HSP 1 Score: 55.1 bits (131), Expect = 3.8e-06
Identity = 34/118 (28.81%), Postives = 66/118 (55.93%), Query Frame = 0

Query: 5   ILGRGFTSNKSQI--KLTKSRIDVIRRKKKATLKFLKKDIADLLANGLDINAYGRAEGLL 64
           +LG GF + + ++  +L  +R+ ++ +KK    +  +K+IAD LA G D  A  R E ++
Sbjct: 1   MLGSGFKAERLRVNLRLVINRLKLLEKKKTELAQKARKEIADYLAAGKDERARIRVEHII 60

Query: 65  VELTISSCYDFVEQSCDTVLKHLPIMQKQRECPEECCEAIASLMFAAARF-SDLPELR 120
            E  +    + +E  CD +L    ++Q  +E      E++++L++AA R  S++ EL+
Sbjct: 61  REDYLVEAMEILELYCDLLLARFGLIQSMKELDSGLAESVSTLIWAAPRLQSEVAELK 118

BLAST of CmaCh10G006080 vs. ExPASy Swiss-Prot
Match: Q568Z6 (IST1 homolog OS=Rattus norvegicus OX=10116 GN=Ist1 PE=2 SV=1)

HSP 1 Score: 55.1 bits (131), Expect = 3.8e-06
Identity = 34/118 (28.81%), Postives = 66/118 (55.93%), Query Frame = 0

Query: 5   ILGRGFTSNKSQI--KLTKSRIDVIRRKKKATLKFLKKDIADLLANGLDINAYGRAEGLL 64
           +LG GF + + ++  +L  +R+ ++ +KK    +  +K+IAD LA G D  A  R E ++
Sbjct: 1   MLGSGFKAERLRVNLRLVINRLKLLEKKKTELAQKARKEIADYLAAGKDERARIRVEHII 60

Query: 65  VELTISSCYDFVEQSCDTVLKHLPIMQKQRECPEECCEAIASLMFAAARF-SDLPELR 120
            E  +    + +E  CD +L    ++Q  +E      E++++L++AA R  S++ EL+
Sbjct: 61  REDYLVEAMEILELYCDLLLARFGLIQSMKELDSGLAESVSTLIWAAPRLQSEVAELK 118

BLAST of CmaCh10G006080 vs. ExPASy TrEMBL
Match: A0A6J1JEU2 (uncharacterized protein LOC111485130 OS=Cucurbita maxima OX=3661 GN=LOC111485130 PE=3 SV=1)

HSP 1 Score: 1381.3 bits (3574), Expect = 0.0e+00
Identity = 703/705 (99.72%), Postives = 703/705 (99.72%), Query Frame = 0

Query: 1   MLDGILGRGFTSN-KSQIKLTKSRIDVIRRKKKATLKFLKKDIADLLANGLDINAYGRAE 60
           MLDGILGRGFTS  KSQIKLTKSRIDVIRRKKKATLKFLKKDIADLLANGLDINAYGRAE
Sbjct: 1   MLDGILGRGFTSKCKSQIKLTKSRIDVIRRKKKATLKFLKKDIADLLANGLDINAYGRAE 60

Query: 61  GLLVELTISSCYDFVEQSCDTVLKHLPIMQKQRECPEECCEAIASLMFAAARFSDLPELR 120
           GLLVELTISSCYDFVEQSCDTVLKHLPIMQKQRECPEECCEAIASLMFAAARFSDLPELR
Sbjct: 61  GLLVELTISSCYDFVEQSCDTVLKHLPIMQKQRECPEECCEAIASLMFAAARFSDLPELR 120

Query: 121 ELRQIFQERFGTSVEHLENRKFVENLASKPSTLEKKVQLLQDIALEFSIKWDSVGFEKRM 180
           ELRQIFQERFGTSVEHLENRKFVENLASKPSTLEKKVQLLQDIALEFSIKWDSVGFEKRM
Sbjct: 121 ELRQIFQERFGTSVEHLENRKFVENLASKPSTLEKKVQLLQDIALEFSIKWDSVGFEKRM 180

Query: 181 STPPAYAQGLPKDFGAHNAAIEKSLSHAKEIDPRVKDDGVSFKENFEQPNARHRFLNPSD 240
           STPPAYAQGLPKDFGAHNAAIEKSLSHAKEIDPRVKDDGVSFKENFEQPNARHRFLNPSD
Sbjct: 181 STPPAYAQGLPKDFGAHNAAIEKSLSHAKEIDPRVKDDGVSFKENFEQPNARHRFLNPSD 240

Query: 241 STIAGGKELKFQPKQEFPRHRHENRVYDEEETILKSDGRINLYGEKKGSTVPKHEARIGR 300
           STIAGGKELKFQPKQEFPRHRHENRVYDEEETILKSDGRINLYGEKKGSTVPKHEARIGR
Sbjct: 241 STIAGGKELKFQPKQEFPRHRHENRVYDEEETILKSDGRINLYGEKKGSTVPKHEARIGR 300

Query: 301 MGSSSSSEVLKDADNGLVVQNGRERRVPDYLKSSPYNNSGLAPTSKHEAGNGMMSSAFGP 360
           MGSSSSSEVLKDADNGLVVQNGRERRVPDYLKSSPYNNSGLAPTSKHEAGNGMMSSAFGP
Sbjct: 301 MGSSSSSEVLKDADNGLVVQNGRERRVPDYLKSSPYNNSGLAPTSKHEAGNGMMSSAFGP 360

Query: 361 RRMGSSSSSEVLGDVDDGTTVVHNDRERTVPNYLKSPPYSKSGLAPSDIKEPSSGNTHTG 420
           RRMGSSSSSEVLGDVDDGTTVVHNDRERTVPNYLKSPPYSKSGLAPSDIKEPSSGNTHTG
Sbjct: 361 RRMGSSSSSEVLGDVDDGTTVVHNDRERTVPNYLKSPPYSKSGLAPSDIKEPSSGNTHTG 420

Query: 421 HGYAVHQGKAEEDKHQNTKPSFNSILPPPYVKANSRRKDNKDRSHDNNCVSMGPQKPVKS 480
           HGYAVHQGKAEEDKHQNTKPSFNSILPPPYVKANSRRKDNKDRSHDNNCVSMGPQKPVKS
Sbjct: 421 HGYAVHQGKAEEDKHQNTKPSFNSILPPPYVKANSRRKDNKDRSHDNNCVSMGPQKPVKS 480

Query: 481 EMITGVELEPRHSDHERQVISPLRASSHGGEMDRFFGGRIPPDALPKPRSVRRRHHKPRS 540
           EMITGVELEPRHSDHERQVISPLRASSHGGEMDRFFGGRIPPDALPKPRSVRRRHHKPRS
Sbjct: 481 EMITGVELEPRHSDHERQVISPLRASSHGGEMDRFFGGRIPPDALPKPRSVRRRHHKPRS 540

Query: 541 SHSVDDNAEDIRTVRKKSRSSSRRRDDKRGLQLLIDEQENERDEEERIIDKLLIHYSKKP 600
           SHSVDDNAEDIRTVRKKSRSSSRRRDDKRGLQLLIDEQENERDEEERIIDKLLIHYSKKP
Sbjct: 541 SHSVDDNAEDIRTVRKKSRSSSRRRDDKRGLQLLIDEQENERDEEERIIDKLLIHYSKKP 600

Query: 601 STFEPGKVRRKPKNHLSLENGTDSAKSPSNLRSRDEADKQADTVHPPARSASLPPEHHLG 660
           STFEPGKVRRKPKNHLSLENGTDSAKSPSNLRSRDEADKQADTVHPPARSASLPPEHHLG
Sbjct: 601 STFEPGKVRRKPKNHLSLENGTDSAKSPSNLRSRDEADKQADTVHPPARSASLPPEHHLG 660

Query: 661 PSETTKVYTRAISFQPDRSSAAKHVHPKLPDYDDLAARFAALRGR 705
           PSETTKVYTRAISFQPDRSSAAKHVHPKLPDYDDLAARFAALRGR
Sbjct: 661 PSETTKVYTRAISFQPDRSSAAKHVHPKLPDYDDLAARFAALRGR 705

BLAST of CmaCh10G006080 vs. ExPASy TrEMBL
Match: A0A6J1HAK9 (uncharacterized protein LOC111461604 OS=Cucurbita moschata OX=3662 GN=LOC111461604 PE=3 SV=1)

HSP 1 Score: 1281.5 bits (3315), Expect = 0.0e+00
Identity = 663/705 (94.04%), Postives = 672/705 (95.32%), Query Frame = 0

Query: 1   MLDGILGRGFTSN-KSQIKLTKSRIDVIRRKKKATLKFLKKDIADLLANGLDINAYGRAE 60
           MLDGILGRGFTS  KSQIKLTKSRIDVIRRKKKATLKFLKKDIADLLANGLDINAYGRAE
Sbjct: 1   MLDGILGRGFTSKCKSQIKLTKSRIDVIRRKKKATLKFLKKDIADLLANGLDINAYGRAE 60

Query: 61  GLLVELTISSCYDFVEQSCDTVLKHLPIMQKQRECPEECCEAIASLMFAAARFSDLPELR 120
           GLLVELTISSCYDFVEQSCDTVLKHLPIMQKQRECPEECCEAIASLMFAAARFSDLPELR
Sbjct: 61  GLLVELTISSCYDFVEQSCDTVLKHLPIMQKQRECPEECCEAIASLMFAAARFSDLPELR 120

Query: 121 ELRQIFQERFGTSVEHLENRKFVENLASKPSTLEKKVQLLQDIALEFSIKWDSVGFEKRM 180
           +LRQIFQERFGTSVEHLENRKFVENLASKPSTLEKKVQLLQDIALEFSIKWDSVGFEKRM
Sbjct: 121 DLRQIFQERFGTSVEHLENRKFVENLASKPSTLEKKVQLLQDIALEFSIKWDSVGFEKRM 180

Query: 181 STPPAYAQGLPKDFGAHNAAIEKSLSHAKEIDPRVKDDGVSFKENFEQPNARHRFLNPSD 240
           STPPAYAQGLPKDFGAHNAAIEKS SHAK IDPRVKD GVS+KENFEQPNARHRFLNP D
Sbjct: 181 STPPAYAQGLPKDFGAHNAAIEKSPSHAKAIDPRVKDGGVSYKENFEQPNARHRFLNPRD 240

Query: 241 STIAGGKELKFQPKQEFPRHRHENRVYDEEETILKSDGRINLYGEKKGSTVPKHEARIGR 300
           STI+GGKELKFQPKQEFPRHRHENRV+DEEETILKSDGRINLYGEKKG     HEA IGR
Sbjct: 241 STISGGKELKFQPKQEFPRHRHENRVHDEEETILKSDGRINLYGEKKG-----HEAGIGR 300

Query: 301 MGSSSSSEVLKDADNGLVVQNGRERRVPDYLKSSPYNNSGLAPTSKHEAGNGMMSSAFGP 360
           MG SSSSEVLKDADN LVVQNGRERRVPDYLKSSPYNN GLAPTSKHEAGNGMMSSAFGP
Sbjct: 301 MG-SSSSEVLKDADNELVVQNGRERRVPDYLKSSPYNNPGLAPTSKHEAGNGMMSSAFGP 360

Query: 361 RRMGSSSSSEVLGDVDDGTTVVHNDRERTVPNYLKSPPYSKSGLAPSDIKEPSSGNTHTG 420
           RRMGSSSSSEVLGDVDDG  VVHN RERTVPNYLKSPPYS  GLAPSDIKEPSSGNTHTG
Sbjct: 361 RRMGSSSSSEVLGDVDDG-RVVHNGRERTVPNYLKSPPYSNPGLAPSDIKEPSSGNTHTG 420

Query: 421 HGYAVHQGKAEEDKHQNTKPSFNSILPPPYVKANSRRKDNKDRSHDNNCVSMGPQKPVKS 480
           HGYAVHQGKAEEDKHQNTKPSFNSILPPPYVKANSRRKDNKDRSHDNNC        VKS
Sbjct: 421 HGYAVHQGKAEEDKHQNTKPSFNSILPPPYVKANSRRKDNKDRSHDNNC--------VKS 480

Query: 481 EMITGVELEPRHSDHERQVISPLRASSHGGEMDRFFGGRIPPDALPKPRSVRRRHHKPRS 540
           EM TGVELEPRHSDHE+QV SP++ASSHGGEMDRFFGGRIPPD LPKPRSVRRRHHKPRS
Sbjct: 481 EMTTGVELEPRHSDHEKQVTSPVKASSHGGEMDRFFGGRIPPDVLPKPRSVRRRHHKPRS 540

Query: 541 SHSVDDNAEDIRTVRKKSRSSSRRRDDKRGLQLLIDEQENERDEEERIIDKLLIHYSKKP 600
           SHSVDDNAEDIRTVRKKSRSSSRRRDDKRGLQLLIDEQENERDEEERIIDKLLIHYSKKP
Sbjct: 541 SHSVDDNAEDIRTVRKKSRSSSRRRDDKRGLQLLIDEQENERDEEERIIDKLLIHYSKKP 600

Query: 601 STFEPGKVRRKPKNHLSLENGTDSAKSPSNLRSRDEADKQADTVHPPARSASLPPEHHLG 660
           STFEPGK+RRKPKN+LSL NGTDSAKSP NLRSRDEADKQADTVHPPARSASLPPEHHLG
Sbjct: 601 STFEPGKIRRKPKNYLSLGNGTDSAKSPLNLRSRDEADKQADTVHPPARSASLPPEHHLG 660

Query: 661 PSETTKVYTRAISFQPDRSSAAKHVHPKLPDYDDLAARFAALRGR 705
           PSETTKVYTRAISFQPDRSSAAKHVHPKLPDYDDLAARFAALRGR
Sbjct: 661 PSETTKVYTRAISFQPDRSSAAKHVHPKLPDYDDLAARFAALRGR 690

BLAST of CmaCh10G006080 vs. ExPASy TrEMBL
Match: A0A5A7TSR7 (IST1 like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold114G001320 PE=3 SV=1)

HSP 1 Score: 1080.9 bits (2794), Expect = 0.0e+00
Identity = 594/754 (78.78%), Postives = 626/754 (83.02%), Query Frame = 0

Query: 1   MLDGILGRGFTSN-KSQIKLTKSRIDVIRRKKKATLKFLKKDIADLLANGLDINAYGRAE 60
           MLDGILGRGFTS  KS IKLTKSRIDVIRRKKKATLKFLKKDIADLLANGLDINAYGRAE
Sbjct: 1   MLDGILGRGFTSKCKSLIKLTKSRIDVIRRKKKATLKFLKKDIADLLANGLDINAYGRAE 60

Query: 61  GLLVELTISSCYDFVEQSCDTVLKHLPIMQKQRECPEECCEAIASLMFAAARFSDLPELR 120
           GLLVELTISSCYDFVEQSCDTVL+HLPIMQKQRECP+ECCEAIASLMFAAARFSDLPELR
Sbjct: 61  GLLVELTISSCYDFVEQSCDTVLQHLPIMQKQRECPDECCEAIASLMFAAARFSDLPELR 120

Query: 121 ELRQIFQERFGTSVEHLENRKFVENLASKPSTLEKKVQLLQDIALEFSIKWDSVGFEKRM 180
           ELRQIFQERFG S+EHLEN+KFVENLASKPSTLEKKVQLLQDIALEFSIKWDSVGFEKRM
Sbjct: 121 ELRQIFQERFGASLEHLENQKFVENLASKPSTLEKKVQLLQDIALEFSIKWDSVGFEKRM 180

Query: 181 STPPAYAQGLPKDFGAHNAAIEKSLSHAKEIDPRV-KDDGVSFKENFEQPNARHRFLNPS 240
           STPPAYAQGLPKD G++NA ++KS SHA+EIDPRV KD GVS+KENFE  N RHRF+NPS
Sbjct: 181 STPPAYAQGLPKDRGSYNAVVDKS-SHAREIDPRVGKDGGVSYKENFEHANGRHRFVNPS 240

Query: 241 DSTIAGGKELKFQPKQEFPRHRHENRVYDEEETILKSDGRINLYGEKKGSTVPKHEA--- 300
           DSTI+GGKE KFQ +QE P H HENR Y +EET +K DGRIN YGEKKGSTV KHEA   
Sbjct: 241 DSTISGGKEPKFQVRQELPGHGHENREYGKEETPMKFDGRINHYGEKKGSTVSKHEARNG 300

Query: 301 ------RIGRMGSSSSSEVLKDADNGLVVQNGRERRVPDYLKSSPYNNSGLAPTSKHEAG 360
                 RIGRMG SSSSEV  DADNGLVV NGR R VP+YLK SPYN  G  P SKHEAG
Sbjct: 301 TVGSSPRIGRMGGSSSSEVPGDADNGLVVHNGRGRAVPEYLK-SPYNIPG-PPISKHEAG 360

Query: 361 NGMMSSAFGPRRMGSSSSSEVLGDVDDGTTVVHNDRERTVPNYLKSPPYSKSGLAP---- 420
           NGMM SAF   RMGSSSSSEVLGD DD   VVHN +ERTVPNYLKS PY+  GLAP    
Sbjct: 361 NGMMGSAFRTSRMGSSSSSEVLGDADD-RPVVHNGQERTVPNYLKSSPYNNPGLAPPSDA 420

Query: 421 -----SDIKEPSSGNTHT---------------------GHGYAVHQGKAEEDKHQNTKP 480
                SDIKE SSGNTHT                     GHGYAV QGK EEDK QN KP
Sbjct: 421 GLQLKSDIKERSSGNTHTGHNGLIFKSDLKDSSFGNTHNGHGYAVLQGKVEEDK-QNLKP 480

Query: 481 SFNSILPPPYVKANSRRKDNKDRS--------HDNNCVSMGPQKPVKSEMIT-GVELEPR 540
           S+NSILPPPYVK NSRRKD+KDRS        HDNNCVS  PQKPVKSEM    ++LEP 
Sbjct: 481 SYNSILPPPYVKPNSRRKDHKDRSHSELSRTGHDNNCVSTDPQKPVKSEMTAPALQLEPS 540

Query: 541 HSDHERQVISPLRASSHGGEMDRFFGGRIPPDALPKPRSVRRRHHKPRSSHSVDDNAEDI 600
           H DHERQV SP+R+SS GGEMD  FG RIPPDALPKPRSVRRRHHKPRSSHS+DDNAEDI
Sbjct: 541 HPDHERQVTSPMRSSSRGGEMDHVFGARIPPDALPKPRSVRRRHHKPRSSHSIDDNAEDI 600

Query: 601 RTVRKKSRSSSRRRDDKRGLQLLIDEQENERDEEERIIDKLLIHYSKKPSTFEPGKVRRK 660
           R VRKKSR SSRRRDDKRGLQLL+DEQENERDEEERIIDKLLIHYSKKPS+FEPGK+RRK
Sbjct: 601 RMVRKKSR-SSRRRDDKRGLQLLVDEQENERDEEERIIDKLLIHYSKKPSSFEPGKMRRK 660

Query: 661 PKNHLSLENGTDSAKSPSNLRSRDEADKQADTVHPPARSASLPPEHHLGPSETTKVYTRA 705
           PK+HL+ ENGTD AKSP NL S   AD QADTV PPARSASLP EH+LG SE TKV+TRA
Sbjct: 661 PKSHLAPENGTDVAKSPLNLISSGGADGQADTVPPPARSASLPREHNLGSSEATKVFTRA 720

BLAST of CmaCh10G006080 vs. ExPASy TrEMBL
Match: A0A1S3CKP3 (uncharacterized protein LOC103501548 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103501548 PE=3 SV=1)

HSP 1 Score: 1080.9 bits (2794), Expect = 0.0e+00
Identity = 594/754 (78.78%), Postives = 626/754 (83.02%), Query Frame = 0

Query: 1   MLDGILGRGFTSN-KSQIKLTKSRIDVIRRKKKATLKFLKKDIADLLANGLDINAYGRAE 60
           MLDGILGRGFTS  KS IKLTKSRIDVIRRKKKATLKFLKKDIADLLANGLDINAYGRAE
Sbjct: 1   MLDGILGRGFTSKCKSLIKLTKSRIDVIRRKKKATLKFLKKDIADLLANGLDINAYGRAE 60

Query: 61  GLLVELTISSCYDFVEQSCDTVLKHLPIMQKQRECPEECCEAIASLMFAAARFSDLPELR 120
           GLLVELTISSCYDFVEQSCDTVL+HLPIMQKQRECP+ECCEAIASLMFAAARFSDLPELR
Sbjct: 61  GLLVELTISSCYDFVEQSCDTVLQHLPIMQKQRECPDECCEAIASLMFAAARFSDLPELR 120

Query: 121 ELRQIFQERFGTSVEHLENRKFVENLASKPSTLEKKVQLLQDIALEFSIKWDSVGFEKRM 180
           ELRQIFQERFG S+EHLEN+KFVENLASKPSTLEKKVQLLQDIALEFSIKWDSVGFEKRM
Sbjct: 121 ELRQIFQERFGASLEHLENQKFVENLASKPSTLEKKVQLLQDIALEFSIKWDSVGFEKRM 180

Query: 181 STPPAYAQGLPKDFGAHNAAIEKSLSHAKEIDPRV-KDDGVSFKENFEQPNARHRFLNPS 240
           STPPAYAQGLPKD G++NA ++KS SHA+EIDPRV KD GVS+KENFE  N RHRF+NPS
Sbjct: 181 STPPAYAQGLPKDRGSYNAVVDKS-SHAREIDPRVGKDGGVSYKENFEHANGRHRFVNPS 240

Query: 241 DSTIAGGKELKFQPKQEFPRHRHENRVYDEEETILKSDGRINLYGEKKGSTVPKHEA--- 300
           DSTI+GGKE KFQ +QE P H HENR Y +EET +K DGRIN YGEKKGSTV KHEA   
Sbjct: 241 DSTISGGKEPKFQVRQELPGHGHENREYGKEETPMKFDGRINHYGEKKGSTVSKHEARNG 300

Query: 301 ------RIGRMGSSSSSEVLKDADNGLVVQNGRERRVPDYLKSSPYNNSGLAPTSKHEAG 360
                 RIGRMG SSSSEV  DADNGLVV NGR R VP+YLK SPYN  G  P SKHEAG
Sbjct: 301 TVGSSPRIGRMGGSSSSEVPGDADNGLVVHNGRGRAVPEYLK-SPYNIPG-PPISKHEAG 360

Query: 361 NGMMSSAFGPRRMGSSSSSEVLGDVDDGTTVVHNDRERTVPNYLKSPPYSKSGLAP---- 420
           NGMM SAF   RMGSSSSSEVLGD DD   VVHN +ERTVPNYLKS PY+  GLAP    
Sbjct: 361 NGMMGSAFRTSRMGSSSSSEVLGDADD-RPVVHNGQERTVPNYLKSSPYNNPGLAPPSDA 420

Query: 421 -----SDIKEPSSGNTHT---------------------GHGYAVHQGKAEEDKHQNTKP 480
                SDIKE SSGNTHT                     GHGYAV QGK EEDK QN KP
Sbjct: 421 GLQLKSDIKERSSGNTHTGHNGLIFKSDLKDSSFGNTHNGHGYAVLQGKVEEDK-QNLKP 480

Query: 481 SFNSILPPPYVKANSRRKDNKDRS--------HDNNCVSMGPQKPVKSEMIT-GVELEPR 540
           S+NSILPPPYVK NSRRKD+KDRS        HDNNCVS  PQKPVKSEM    ++LEP 
Sbjct: 481 SYNSILPPPYVKPNSRRKDHKDRSHSELSRTGHDNNCVSTDPQKPVKSEMTAPALQLEPS 540

Query: 541 HSDHERQVISPLRASSHGGEMDRFFGGRIPPDALPKPRSVRRRHHKPRSSHSVDDNAEDI 600
           H DHERQV SP+R+SS GGEMD  FG RIPPDALPKPRSVRRRHHKPRSSHS+DDNAEDI
Sbjct: 541 HPDHERQVTSPMRSSSRGGEMDHVFGARIPPDALPKPRSVRRRHHKPRSSHSIDDNAEDI 600

Query: 601 RTVRKKSRSSSRRRDDKRGLQLLIDEQENERDEEERIIDKLLIHYSKKPSTFEPGKVRRK 660
           R VRKKSR SSRRRDDKRGLQLL+DEQENERDEEERIIDKLLIHYSKKPS+FEPGK+RRK
Sbjct: 601 RMVRKKSR-SSRRRDDKRGLQLLVDEQENERDEEERIIDKLLIHYSKKPSSFEPGKMRRK 660

Query: 661 PKNHLSLENGTDSAKSPSNLRSRDEADKQADTVHPPARSASLPPEHHLGPSETTKVYTRA 705
           PK+HL+ ENGTD AKSP NL S   AD QADTV PPARSASLP EH+LG SE TKV+TRA
Sbjct: 661 PKSHLAPENGTDVAKSPLNLISSGGADGQADTVPPPARSASLPREHNLGSSEATKVFTRA 720

BLAST of CmaCh10G006080 vs. ExPASy TrEMBL
Match: A0A5D3BK14 (IST1 like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold596G00590 PE=3 SV=1)

HSP 1 Score: 1073.5 bits (2775), Expect = 2.6e-310
Identity = 594/754 (78.78%), Postives = 624/754 (82.76%), Query Frame = 0

Query: 1   MLDGILGRGFTSN-KSQIKLTKSRIDVIRRKKKATLKFLKKDIADLLANGLDINAYGRAE 60
           MLDGILGRGFTS  KS IKLTKSRIDVIRRKKKATLKFLKKDIADLLANGLDINAYGRAE
Sbjct: 1   MLDGILGRGFTSKCKSLIKLTKSRIDVIRRKKKATLKFLKKDIADLLANGLDINAYGRAE 60

Query: 61  GLLVELTISSCYDFVEQSCDTVLKHLPIMQKQRECPEECCEAIASLMFAAARFSDLPELR 120
           GLLVELTISSCYDFVEQSCDTVL+HLPIMQKQRECP+ECCEAIASLMFAAARFSDLPELR
Sbjct: 61  GLLVELTISSCYDFVEQSCDTVLQHLPIMQKQRECPDECCEAIASLMFAAARFSDLPELR 120

Query: 121 ELRQIFQERFGTSVEHLENRKFVENLASKPSTLEKKVQLLQDIALEFSIKWDSVGFEKRM 180
           ELRQIFQERFG S+EHLEN+KFVENLASKPSTLEKKVQLLQDIALEFSIKWDSVGFEKRM
Sbjct: 121 ELRQIFQERFGASLEHLENQKFVENLASKPSTLEKKVQLLQDIALEFSIKWDSVGFEKRM 180

Query: 181 STPPAYAQGLPKDFGAHNAAIEKSLSHAKEIDPRV-KDDGVSFKENFEQPNARHRFLNPS 240
           STPPAYAQGLPKD G++NA ++KS SHAKEIDPRV KD GVS+KENFE  N RHRF+NPS
Sbjct: 181 STPPAYAQGLPKDRGSYNAVVDKS-SHAKEIDPRVGKDGGVSYKENFEHANGRHRFVNPS 240

Query: 241 DSTIAGGKELKFQPKQEFPRHRHENRVYDEEETILKSDGRINLYGEKKGSTVPKHEA--- 300
           DSTI+GGKE KFQ +QE P H HENR Y +EET +K DGRIN YGEKKGSTV KHEA   
Sbjct: 241 DSTISGGKEPKFQVRQELPGHGHENREYGKEETPMKFDGRINHYGEKKGSTVSKHEARNG 300

Query: 301 ------RIGRMGSSSSSEVLKDADNGLVVQNGRERRVPDYLKSSPYNNSGLAPTSKHEAG 360
                 RIGRMG SSSSEV  DADNGLVV NGR R VP+YLK SPYN  G  P SKHEAG
Sbjct: 301 TVGSSPRIGRMGGSSSSEVPGDADNGLVVHNGRGRAVPEYLK-SPYNIPG-PPISKHEAG 360

Query: 361 NGMMSSAFGPRRMGSSSSSEVLGDVDDGTTVVHNDRERTVPNYLKSPPYSKSGLAP---- 420
           NGMM SAF   RMGSSSSSEVLGD DD   VVHN +ERTVPNYLKS PY+  GLAP    
Sbjct: 361 NGMMGSAFRTSRMGSSSSSEVLGDADD-RPVVHNGQERTVPNYLKSSPYNNPGLAPPSDA 420

Query: 421 -----SDIKEPSSGNTHT---------------------GHGYAVHQGKAEEDKHQNTKP 480
                SDIKE SSGNTHT                     GHGYAV QGK EEDK QN KP
Sbjct: 421 GLQLKSDIKERSSGNTHTGHNGLIFKSDLKDSSFGNTHNGHGYAVLQGKVEEDK-QNLKP 480

Query: 481 SFNSILPPPYVKANSRRKDNKDRS--------HDNNCVSMGPQKPVKSEMIT-GVELEPR 540
           S+NSIL PPYVK NSRRKD KDRS        HDNNCVS  PQKPVKSEM    ++LEP 
Sbjct: 481 SYNSIL-PPYVKPNSRRKDLKDRSHSELSRTGHDNNCVSTDPQKPVKSEMTAPALQLEPS 540

Query: 541 HSDHERQVISPLRASSHGGEMDRFFGGRIPPDALPKPRSVRRRHHKPRSSHSVDDNAEDI 600
           H DHERQV SP+R+SS GGEMD  FG RIPPDALPKPRSVRRRHHKPRSSHS+DDNAEDI
Sbjct: 541 HPDHERQVTSPMRSSSRGGEMDHVFGARIPPDALPKPRSVRRRHHKPRSSHSIDDNAEDI 600

Query: 601 RTVRKKSRSSSRRRDDKRGLQLLIDEQENERDEEERIIDKLLIHYSKKPSTFEPGKVRRK 660
           R VRKKSR SSRRRDDKRGLQLL+DEQENERDEEERIIDKLLIHYSKKPS+FEPGK+RRK
Sbjct: 601 RMVRKKSR-SSRRRDDKRGLQLLVDEQENERDEEERIIDKLLIHYSKKPSSFEPGKMRRK 660

Query: 661 PKNHLSLENGTDSAKSPSNLRSRDEADKQADTVHPPARSASLPPEHHLGPSETTKVYTRA 705
           PK+HL+ ENGTD AKSP NL S   AD QADTV PPARSASLP EH+LG SE TKV+TRA
Sbjct: 661 PKSHLAPENGTDVAKSPLNLISSGGADGQADTVPPPARSASLPREHNLGSSEATKVFTRA 720

BLAST of CmaCh10G006080 vs. NCBI nr
Match: XP_022987631.1 (uncharacterized protein LOC111485130 [Cucurbita maxima])

HSP 1 Score: 1381.3 bits (3574), Expect = 0.0e+00
Identity = 703/705 (99.72%), Postives = 703/705 (99.72%), Query Frame = 0

Query: 1   MLDGILGRGFTSN-KSQIKLTKSRIDVIRRKKKATLKFLKKDIADLLANGLDINAYGRAE 60
           MLDGILGRGFTS  KSQIKLTKSRIDVIRRKKKATLKFLKKDIADLLANGLDINAYGRAE
Sbjct: 1   MLDGILGRGFTSKCKSQIKLTKSRIDVIRRKKKATLKFLKKDIADLLANGLDINAYGRAE 60

Query: 61  GLLVELTISSCYDFVEQSCDTVLKHLPIMQKQRECPEECCEAIASLMFAAARFSDLPELR 120
           GLLVELTISSCYDFVEQSCDTVLKHLPIMQKQRECPEECCEAIASLMFAAARFSDLPELR
Sbjct: 61  GLLVELTISSCYDFVEQSCDTVLKHLPIMQKQRECPEECCEAIASLMFAAARFSDLPELR 120

Query: 121 ELRQIFQERFGTSVEHLENRKFVENLASKPSTLEKKVQLLQDIALEFSIKWDSVGFEKRM 180
           ELRQIFQERFGTSVEHLENRKFVENLASKPSTLEKKVQLLQDIALEFSIKWDSVGFEKRM
Sbjct: 121 ELRQIFQERFGTSVEHLENRKFVENLASKPSTLEKKVQLLQDIALEFSIKWDSVGFEKRM 180

Query: 181 STPPAYAQGLPKDFGAHNAAIEKSLSHAKEIDPRVKDDGVSFKENFEQPNARHRFLNPSD 240
           STPPAYAQGLPKDFGAHNAAIEKSLSHAKEIDPRVKDDGVSFKENFEQPNARHRFLNPSD
Sbjct: 181 STPPAYAQGLPKDFGAHNAAIEKSLSHAKEIDPRVKDDGVSFKENFEQPNARHRFLNPSD 240

Query: 241 STIAGGKELKFQPKQEFPRHRHENRVYDEEETILKSDGRINLYGEKKGSTVPKHEARIGR 300
           STIAGGKELKFQPKQEFPRHRHENRVYDEEETILKSDGRINLYGEKKGSTVPKHEARIGR
Sbjct: 241 STIAGGKELKFQPKQEFPRHRHENRVYDEEETILKSDGRINLYGEKKGSTVPKHEARIGR 300

Query: 301 MGSSSSSEVLKDADNGLVVQNGRERRVPDYLKSSPYNNSGLAPTSKHEAGNGMMSSAFGP 360
           MGSSSSSEVLKDADNGLVVQNGRERRVPDYLKSSPYNNSGLAPTSKHEAGNGMMSSAFGP
Sbjct: 301 MGSSSSSEVLKDADNGLVVQNGRERRVPDYLKSSPYNNSGLAPTSKHEAGNGMMSSAFGP 360

Query: 361 RRMGSSSSSEVLGDVDDGTTVVHNDRERTVPNYLKSPPYSKSGLAPSDIKEPSSGNTHTG 420
           RRMGSSSSSEVLGDVDDGTTVVHNDRERTVPNYLKSPPYSKSGLAPSDIKEPSSGNTHTG
Sbjct: 361 RRMGSSSSSEVLGDVDDGTTVVHNDRERTVPNYLKSPPYSKSGLAPSDIKEPSSGNTHTG 420

Query: 421 HGYAVHQGKAEEDKHQNTKPSFNSILPPPYVKANSRRKDNKDRSHDNNCVSMGPQKPVKS 480
           HGYAVHQGKAEEDKHQNTKPSFNSILPPPYVKANSRRKDNKDRSHDNNCVSMGPQKPVKS
Sbjct: 421 HGYAVHQGKAEEDKHQNTKPSFNSILPPPYVKANSRRKDNKDRSHDNNCVSMGPQKPVKS 480

Query: 481 EMITGVELEPRHSDHERQVISPLRASSHGGEMDRFFGGRIPPDALPKPRSVRRRHHKPRS 540
           EMITGVELEPRHSDHERQVISPLRASSHGGEMDRFFGGRIPPDALPKPRSVRRRHHKPRS
Sbjct: 481 EMITGVELEPRHSDHERQVISPLRASSHGGEMDRFFGGRIPPDALPKPRSVRRRHHKPRS 540

Query: 541 SHSVDDNAEDIRTVRKKSRSSSRRRDDKRGLQLLIDEQENERDEEERIIDKLLIHYSKKP 600
           SHSVDDNAEDIRTVRKKSRSSSRRRDDKRGLQLLIDEQENERDEEERIIDKLLIHYSKKP
Sbjct: 541 SHSVDDNAEDIRTVRKKSRSSSRRRDDKRGLQLLIDEQENERDEEERIIDKLLIHYSKKP 600

Query: 601 STFEPGKVRRKPKNHLSLENGTDSAKSPSNLRSRDEADKQADTVHPPARSASLPPEHHLG 660
           STFEPGKVRRKPKNHLSLENGTDSAKSPSNLRSRDEADKQADTVHPPARSASLPPEHHLG
Sbjct: 601 STFEPGKVRRKPKNHLSLENGTDSAKSPSNLRSRDEADKQADTVHPPARSASLPPEHHLG 660

Query: 661 PSETTKVYTRAISFQPDRSSAAKHVHPKLPDYDDLAARFAALRGR 705
           PSETTKVYTRAISFQPDRSSAAKHVHPKLPDYDDLAARFAALRGR
Sbjct: 661 PSETTKVYTRAISFQPDRSSAAKHVHPKLPDYDDLAARFAALRGR 705

BLAST of CmaCh10G006080 vs. NCBI nr
Match: KAG6589906.1 (IST1-like protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1303.1 bits (3371), Expect = 0.0e+00
Identity = 673/705 (95.46%), Postives = 676/705 (95.89%), Query Frame = 0

Query: 1   MLDGILGRGFTSN-KSQIKLTKSRIDVIRRKKKATLKFLKKDIADLLANGLDINAYGRAE 60
           MLDGILGRGFTS  KSQIKLTKSRIDVIRRKKKATLKFLKKDIADLLANGLDINAYGRAE
Sbjct: 1   MLDGILGRGFTSKCKSQIKLTKSRIDVIRRKKKATLKFLKKDIADLLANGLDINAYGRAE 60

Query: 61  GLLVELTISSCYDFVEQSCDTVLKHLPIMQKQRECPEECCEAIASLMFAAARFSDLPELR 120
           GLLVELTISSCYDFVEQSCDTVLKHLPIMQKQRECPEECCEAIASLMFAAARFSDLPELR
Sbjct: 61  GLLVELTISSCYDFVEQSCDTVLKHLPIMQKQRECPEECCEAIASLMFAAARFSDLPELR 120

Query: 121 ELRQIFQERFGTSVEHLENRKFVENLASKPSTLEKKVQLLQDIALEFSIKWDSVGFEKRM 180
           ELRQIFQERFGTSVEHLENRKFVENLASKPSTLEKKVQLLQDIALEFSIKWDSVGFEKRM
Sbjct: 121 ELRQIFQERFGTSVEHLENRKFVENLASKPSTLEKKVQLLQDIALEFSIKWDSVGFEKRM 180

Query: 181 STPPAYAQGLPKDFGAHNAAIEKSLSHAKEIDPRVKDDGVSFKENFEQPNARHRFLNPSD 240
           STPPAYAQGLPKDFGAHNAAIEKS SHAKEIDPRVKD GVSFKENFEQPNARHRFLNPSD
Sbjct: 181 STPPAYAQGLPKDFGAHNAAIEKSPSHAKEIDPRVKDGGVSFKENFEQPNARHRFLNPSD 240

Query: 241 STIAGGKELKFQPKQEFPRHRHENRVYDEEETILKSDGRINLYGEKKGSTVPKHEARIGR 300
           STI+GGKELKFQPK EFPRHRHENRVYDEEETILKSDGRINLYGEKKG     HEARIGR
Sbjct: 241 STISGGKELKFQPKHEFPRHRHENRVYDEEETILKSDGRINLYGEKKG-----HEARIGR 300

Query: 301 MGSSSSSEVLKDADNGLVVQNGRERRVPDYLKSSPYNNSGLAPTSKHEAGNGMMSSAFGP 360
           MGSSSSSEVLKDADN LVVQNGRERRVPDYLKSSPYNN GLAPTSKHEAGNGMMSSAFGP
Sbjct: 301 MGSSSSSEVLKDADNELVVQNGRERRVPDYLKSSPYNNPGLAPTSKHEAGNGMMSSAFGP 360

Query: 361 RRMGSSSSSEVLGDVDDGTTVVHNDRERTVPNYLKSPPYSKSGLAPSDIKEPSSGNTHTG 420
           RRMGSSSSSEVLGDVDDG  VVHN RERTVPNYLKSPPYS  GLAPSDIKEPSSGNTHTG
Sbjct: 361 RRMGSSSSSEVLGDVDDG-RVVHNGRERTVPNYLKSPPYSNPGLAPSDIKEPSSGNTHTG 420

Query: 421 HGYAVHQGKAEEDKHQNTKPSFNSILPPPYVKANSRRKDNKDRSHDNNCVSMGPQKPVKS 480
           HGYAVHQGKAEEDKHQNTKPSFNSILPPPYVKANSRRKDNKDRSHDNNCV M PQKPVKS
Sbjct: 421 HGYAVHQGKAEEDKHQNTKPSFNSILPPPYVKANSRRKDNKDRSHDNNCVEMDPQKPVKS 480

Query: 481 EMITGVELEPRHSDHERQVISPLRASSHGGEMDRFFGGRIPPDALPKPRSVRRRHHKPRS 540
           EM TGVELEPRHSDHERQVI      SHGGEMDRFFGGRIPPDALPKPRSVRRRHHKPRS
Sbjct: 481 EMTTGVELEPRHSDHERQVI------SHGGEMDRFFGGRIPPDALPKPRSVRRRHHKPRS 540

Query: 541 SHSVDDNAEDIRTVRKKSRSSSRRRDDKRGLQLLIDEQENERDEEERIIDKLLIHYSKKP 600
           SHSVDDNAEDIRTVRKKSRSSSRRRDDKRGLQLLIDEQE+ERDEEERIIDKLLIHYSKKP
Sbjct: 541 SHSVDDNAEDIRTVRKKSRSSSRRRDDKRGLQLLIDEQESERDEEERIIDKLLIHYSKKP 600

Query: 601 STFEPGKVRRKPKNHLSLENGTDSAKSPSNLRSRDEADKQADTVHPPARSASLPPEHHLG 660
           STFEPGK+RRKPKNHLSL NGTDSAKSP NLRSRDEA KQADTVHPPARSASLPPEHHLG
Sbjct: 601 STFEPGKIRRKPKNHLSLGNGTDSAKSPLNLRSRDEAGKQADTVHPPARSASLPPEHHLG 660

Query: 661 PSETTKVYTRAISFQPDRSSAAKHVHPKLPDYDDLAARFAALRGR 705
           PSETTKVYTRAISFQPDRSSAAKHVHPKLPDYDDLAARFAALRGR
Sbjct: 661 PSETTKVYTRAISFQPDRSSAAKHVHPKLPDYDDLAARFAALRGR 693

BLAST of CmaCh10G006080 vs. NCBI nr
Match: XP_023516086.1 (uncharacterized protein LOC111780056 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1290.4 bits (3338), Expect = 0.0e+00
Identity = 667/705 (94.61%), Postives = 672/705 (95.32%), Query Frame = 0

Query: 1   MLDGILGRGFTSN-KSQIKLTKSRIDVIRRKKKATLKFLKKDIADLLANGLDINAYGRAE 60
           MLDGILGRGFTS  KSQIKLTKSRIDVIRRKKKATLKFLKKDIADLLANGLDINAYGRAE
Sbjct: 1   MLDGILGRGFTSKCKSQIKLTKSRIDVIRRKKKATLKFLKKDIADLLANGLDINAYGRAE 60

Query: 61  GLLVELTISSCYDFVEQSCDTVLKHLPIMQKQRECPEECCEAIASLMFAAARFSDLPELR 120
           GLLVELTISSCYDFVEQSCDTVLKHLPIMQKQRECPEECCEAIASLMFAAARFSDLPELR
Sbjct: 61  GLLVELTISSCYDFVEQSCDTVLKHLPIMQKQRECPEECCEAIASLMFAAARFSDLPELR 120

Query: 121 ELRQIFQERFGTSVEHLENRKFVENLASKPSTLEKKVQLLQDIALEFSIKWDSVGFEKRM 180
           ELRQIFQERFGTSVEHLENRKFVENLASKPSTLEKKVQLLQDIALEFSIKWDSVGFEKRM
Sbjct: 121 ELRQIFQERFGTSVEHLENRKFVENLASKPSTLEKKVQLLQDIALEFSIKWDSVGFEKRM 180

Query: 181 STPPAYAQGLPKDFGAHNAAIEKSLSHAKEIDPRVKDDGVSFKENFEQPNARHRFLNPSD 240
           STPPAYAQGLPKDFGAHNAAIEKS SHAK IDPRVKD GVS++E FE+ NARHRFLNPSD
Sbjct: 181 STPPAYAQGLPKDFGAHNAAIEKSPSHAKAIDPRVKDGGVSYREKFEEANARHRFLNPSD 240

Query: 241 STIAGGKELKFQPKQEFPRHRHENRVYDEEETILKSDGRINLYGEKKGSTVPKHEARIGR 300
           STI GGKELKFQPKQEFPRHRHENRVYDEEETILKSDGRINLYGEKKG     HEARIGR
Sbjct: 241 STIVGGKELKFQPKQEFPRHRHENRVYDEEETILKSDGRINLYGEKKG-----HEARIGR 300

Query: 301 MGSSSSSEVLKDADNGLVVQNGRERRVPDYLKSSPYNNSGLAPTSKHEAGNGMMSSAFGP 360
           MGSSSSSEV KDADN LVVQNGRERRVPDYLKSSPYNN GLAPTSKHEAGNGMMSSAFGP
Sbjct: 301 MGSSSSSEVQKDADNELVVQNGRERRVPDYLKSSPYNNPGLAPTSKHEAGNGMMSSAFGP 360

Query: 361 RRMGSSSSSEVLGDVDDGTTVVHNDRERTVPNYLKSPPYSKSGLAPSDIKEPSSGNTHTG 420
           RRMGSSSSSEVLGDVDDG  VVHN RERTVPNYLKSPPYS  GLAPS+IKEPSSGNTHTG
Sbjct: 361 RRMGSSSSSEVLGDVDDG-RVVHNSRERTVPNYLKSPPYSNPGLAPSNIKEPSSGNTHTG 420

Query: 421 HGYAVHQGKAEEDKHQNTKPSFNSILPPPYVKANSRRKDNKDRSHDNNCVSMGPQKPVKS 480
           HGYAVHQGKAEEDKHQNTKPSFNSILPPPYVKANSRRKDNKDRSHDNNC        VKS
Sbjct: 421 HGYAVHQGKAEEDKHQNTKPSFNSILPPPYVKANSRRKDNKDRSHDNNC--------VKS 480

Query: 481 EMITGVELEPRHSDHERQVISPLRASSHGGEMDRFFGGRIPPDALPKPRSVRRRHHKPRS 540
           EM TGVELEPRHSDHERQVISPLRASSHGGEMD FFGGRIPPDALPKPRSVRRRHHKPRS
Sbjct: 481 EMTTGVELEPRHSDHERQVISPLRASSHGGEMDHFFGGRIPPDALPKPRSVRRRHHKPRS 540

Query: 541 SHSVDDNAEDIRTVRKKSRSSSRRRDDKRGLQLLIDEQENERDEEERIIDKLLIHYSKKP 600
           SHSVDDNAEDIRTVRKKSRSSSRRRDDKRGLQLLIDEQENERDEEERIIDKLLIHYSKKP
Sbjct: 541 SHSVDDNAEDIRTVRKKSRSSSRRRDDKRGLQLLIDEQENERDEEERIIDKLLIHYSKKP 600

Query: 601 STFEPGKVRRKPKNHLSLENGTDSAKSPSNLRSRDEADKQADTVHPPARSASLPPEHHLG 660
           STFEPGK+RRKPKNHLSL NGTDSAKSP NLRSRDEADKQADTVHPPARSASLPPEHHLG
Sbjct: 601 STFEPGKIRRKPKNHLSLGNGTDSAKSPLNLRSRDEADKQADTVHPPARSASLPPEHHLG 660

Query: 661 PSETTKVYTRAISFQPDRSSAAKHVHPKLPDYDDLAARFAALRGR 705
           PSETTKVYTRAISFQPDRSSAAKHVHPKLPDYDDLAARFAALRGR
Sbjct: 661 PSETTKVYTRAISFQPDRSSAAKHVHPKLPDYDDLAARFAALRGR 691

BLAST of CmaCh10G006080 vs. NCBI nr
Match: XP_022960960.1 (uncharacterized protein LOC111461604 [Cucurbita moschata])

HSP 1 Score: 1281.5 bits (3315), Expect = 0.0e+00
Identity = 663/705 (94.04%), Postives = 672/705 (95.32%), Query Frame = 0

Query: 1   MLDGILGRGFTSN-KSQIKLTKSRIDVIRRKKKATLKFLKKDIADLLANGLDINAYGRAE 60
           MLDGILGRGFTS  KSQIKLTKSRIDVIRRKKKATLKFLKKDIADLLANGLDINAYGRAE
Sbjct: 1   MLDGILGRGFTSKCKSQIKLTKSRIDVIRRKKKATLKFLKKDIADLLANGLDINAYGRAE 60

Query: 61  GLLVELTISSCYDFVEQSCDTVLKHLPIMQKQRECPEECCEAIASLMFAAARFSDLPELR 120
           GLLVELTISSCYDFVEQSCDTVLKHLPIMQKQRECPEECCEAIASLMFAAARFSDLPELR
Sbjct: 61  GLLVELTISSCYDFVEQSCDTVLKHLPIMQKQRECPEECCEAIASLMFAAARFSDLPELR 120

Query: 121 ELRQIFQERFGTSVEHLENRKFVENLASKPSTLEKKVQLLQDIALEFSIKWDSVGFEKRM 180
           +LRQIFQERFGTSVEHLENRKFVENLASKPSTLEKKVQLLQDIALEFSIKWDSVGFEKRM
Sbjct: 121 DLRQIFQERFGTSVEHLENRKFVENLASKPSTLEKKVQLLQDIALEFSIKWDSVGFEKRM 180

Query: 181 STPPAYAQGLPKDFGAHNAAIEKSLSHAKEIDPRVKDDGVSFKENFEQPNARHRFLNPSD 240
           STPPAYAQGLPKDFGAHNAAIEKS SHAK IDPRVKD GVS+KENFEQPNARHRFLNP D
Sbjct: 181 STPPAYAQGLPKDFGAHNAAIEKSPSHAKAIDPRVKDGGVSYKENFEQPNARHRFLNPRD 240

Query: 241 STIAGGKELKFQPKQEFPRHRHENRVYDEEETILKSDGRINLYGEKKGSTVPKHEARIGR 300
           STI+GGKELKFQPKQEFPRHRHENRV+DEEETILKSDGRINLYGEKKG     HEA IGR
Sbjct: 241 STISGGKELKFQPKQEFPRHRHENRVHDEEETILKSDGRINLYGEKKG-----HEAGIGR 300

Query: 301 MGSSSSSEVLKDADNGLVVQNGRERRVPDYLKSSPYNNSGLAPTSKHEAGNGMMSSAFGP 360
           MG SSSSEVLKDADN LVVQNGRERRVPDYLKSSPYNN GLAPTSKHEAGNGMMSSAFGP
Sbjct: 301 MG-SSSSEVLKDADNELVVQNGRERRVPDYLKSSPYNNPGLAPTSKHEAGNGMMSSAFGP 360

Query: 361 RRMGSSSSSEVLGDVDDGTTVVHNDRERTVPNYLKSPPYSKSGLAPSDIKEPSSGNTHTG 420
           RRMGSSSSSEVLGDVDDG  VVHN RERTVPNYLKSPPYS  GLAPSDIKEPSSGNTHTG
Sbjct: 361 RRMGSSSSSEVLGDVDDG-RVVHNGRERTVPNYLKSPPYSNPGLAPSDIKEPSSGNTHTG 420

Query: 421 HGYAVHQGKAEEDKHQNTKPSFNSILPPPYVKANSRRKDNKDRSHDNNCVSMGPQKPVKS 480
           HGYAVHQGKAEEDKHQNTKPSFNSILPPPYVKANSRRKDNKDRSHDNNC        VKS
Sbjct: 421 HGYAVHQGKAEEDKHQNTKPSFNSILPPPYVKANSRRKDNKDRSHDNNC--------VKS 480

Query: 481 EMITGVELEPRHSDHERQVISPLRASSHGGEMDRFFGGRIPPDALPKPRSVRRRHHKPRS 540
           EM TGVELEPRHSDHE+QV SP++ASSHGGEMDRFFGGRIPPD LPKPRSVRRRHHKPRS
Sbjct: 481 EMTTGVELEPRHSDHEKQVTSPVKASSHGGEMDRFFGGRIPPDVLPKPRSVRRRHHKPRS 540

Query: 541 SHSVDDNAEDIRTVRKKSRSSSRRRDDKRGLQLLIDEQENERDEEERIIDKLLIHYSKKP 600
           SHSVDDNAEDIRTVRKKSRSSSRRRDDKRGLQLLIDEQENERDEEERIIDKLLIHYSKKP
Sbjct: 541 SHSVDDNAEDIRTVRKKSRSSSRRRDDKRGLQLLIDEQENERDEEERIIDKLLIHYSKKP 600

Query: 601 STFEPGKVRRKPKNHLSLENGTDSAKSPSNLRSRDEADKQADTVHPPARSASLPPEHHLG 660
           STFEPGK+RRKPKN+LSL NGTDSAKSP NLRSRDEADKQADTVHPPARSASLPPEHHLG
Sbjct: 601 STFEPGKIRRKPKNYLSLGNGTDSAKSPLNLRSRDEADKQADTVHPPARSASLPPEHHLG 660

Query: 661 PSETTKVYTRAISFQPDRSSAAKHVHPKLPDYDDLAARFAALRGR 705
           PSETTKVYTRAISFQPDRSSAAKHVHPKLPDYDDLAARFAALRGR
Sbjct: 661 PSETTKVYTRAISFQPDRSSAAKHVHPKLPDYDDLAARFAALRGR 690

BLAST of CmaCh10G006080 vs. NCBI nr
Match: KAG7023576.1 (IST1-like protein [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1271.9 bits (3290), Expect = 0.0e+00
Identity = 662/705 (93.90%), Postives = 664/705 (94.18%), Query Frame = 0

Query: 1   MLDGILGRGFTSN-KSQIKLTKSRIDVIRRKKKATLKFLKKDIADLLANGLDINAYGRAE 60
           MLDGILGRGFTS  KSQIKLTKSRIDVIRRKKKATLKFLKKDIADLLANGLDINAYGRAE
Sbjct: 1   MLDGILGRGFTSKCKSQIKLTKSRIDVIRRKKKATLKFLKKDIADLLANGLDINAYGRAE 60

Query: 61  GLLVELTISSCYDFVEQSCDTVLKHLPIMQKQRECPEECCEAIASLMFAAARFSDLPELR 120
           GLLVELTISSCYDFVEQSCDTV           ECPEECCEAIASLMFAAARFSDLPELR
Sbjct: 61  GLLVELTISSCYDFVEQSCDTV-----------ECPEECCEAIASLMFAAARFSDLPELR 120

Query: 121 ELRQIFQERFGTSVEHLENRKFVENLASKPSTLEKKVQLLQDIALEFSIKWDSVGFEKRM 180
           ELRQIFQERFGTSVEHLENRKFVENLASKPSTLEKKVQLLQDIALEFSIKWDSVGFEKRM
Sbjct: 121 ELRQIFQERFGTSVEHLENRKFVENLASKPSTLEKKVQLLQDIALEFSIKWDSVGFEKRM 180

Query: 181 STPPAYAQGLPKDFGAHNAAIEKSLSHAKEIDPRVKDDGVSFKENFEQPNARHRFLNPSD 240
           STPPAYAQGLPKDFGAHNAAIEKS SHAKEIDPRVKD GVSFKENFEQPNA HRFLNPSD
Sbjct: 181 STPPAYAQGLPKDFGAHNAAIEKSPSHAKEIDPRVKDGGVSFKENFEQPNASHRFLNPSD 240

Query: 241 STIAGGKELKFQPKQEFPRHRHENRVYDEEETILKSDGRINLYGEKKGSTVPKHEARIGR 300
           STI+GGKELKFQPK EFPRHRHENRVYDEEETILKSDGRINLYGEKKG     HEARIGR
Sbjct: 241 STISGGKELKFQPKHEFPRHRHENRVYDEEETILKSDGRINLYGEKKG-----HEARIGR 300

Query: 301 MGSSSSSEVLKDADNGLVVQNGRERRVPDYLKSSPYNNSGLAPTSKHEAGNGMMSSAFGP 360
           MGSSSSSEVLKDADN LVVQNGRERRVPDYLKSSPYNN GLAPTSKHEAGNGMMSSAFGP
Sbjct: 301 MGSSSSSEVLKDADNELVVQNGRERRVPDYLKSSPYNNPGLAPTSKHEAGNGMMSSAFGP 360

Query: 361 RRMGSSSSSEVLGDVDDGTTVVHNDRERTVPNYLKSPPYSKSGLAPSDIKEPSSGNTHTG 420
           RRMGSSSSSEVLGDVDDG  VVHN RERTVPNYLKSPPYS  GLAPSDIKEPSSGNTHTG
Sbjct: 361 RRMGSSSSSEVLGDVDDG-RVVHNGRERTVPNYLKSPPYSNPGLAPSDIKEPSSGNTHTG 420

Query: 421 HGYAVHQGKAEEDKHQNTKPSFNSILPPPYVKANSRRKDNKDRSHDNNCVSMGPQKPVKS 480
           HGYAVHQGKAEEDKHQNTKPSFNSILPPPYVKANSRRKDNKDRSHDNNCV M PQKPVKS
Sbjct: 421 HGYAVHQGKAEEDKHQNTKPSFNSILPPPYVKANSRRKDNKDRSHDNNCVEMDPQKPVKS 480

Query: 481 EMITGVELEPRHSDHERQVISPLRASSHGGEMDRFFGGRIPPDALPKPRSVRRRHHKPRS 540
           EM TGVELEPRHSDHERQVI      SHGGEMDRFFGGRIPPDALPKPRSVRRRHHKPRS
Sbjct: 481 EMTTGVELEPRHSDHERQVI------SHGGEMDRFFGGRIPPDALPKPRSVRRRHHKPRS 540

Query: 541 SHSVDDNAEDIRTVRKKSRSSSRRRDDKRGLQLLIDEQENERDEEERIIDKLLIHYSKKP 600
           SHSVDDNAEDIRTVRKKSRSSSRRRDDKRGLQLLIDEQENERDEEERIIDKLLIHYSKKP
Sbjct: 541 SHSVDDNAEDIRTVRKKSRSSSRRRDDKRGLQLLIDEQENERDEEERIIDKLLIHYSKKP 600

Query: 601 STFEPGKVRRKPKNHLSLENGTDSAKSPSNLRSRDEADKQADTVHPPARSASLPPEHHLG 660
           STFEPGK+RRKPKNHLSL NGTDSAKSP NLRSRDEA KQADTVHPPARSASLPPEHHLG
Sbjct: 601 STFEPGKIRRKPKNHLSLGNGTDSAKSPLNLRSRDEAGKQADTVHPPARSASLPPEHHLG 660

Query: 661 PSETTKVYTRAISFQPDRSSAAKHVHPKLPDYDDLAARFAALRGR 705
           PSETTKVYTRAISFQPDRSSAAKHVHPKLPDYDDLAARFAALRGR
Sbjct: 661 PSETTKVYTRAISFQPDRSSAAKHVHPKLPDYDDLAARFAALRGR 682

BLAST of CmaCh10G006080 vs. TAIR 10
Match: AT4G32350.1 (Regulator of Vps4 activity in the MVB pathway protein )

HSP 1 Score: 333.2 bits (853), Expect = 5.1e-91
Identity = 283/797 (35.51%), Postives = 389/797 (48.81%), Query Frame = 0

Query: 1   MLDGILGRGFT-SNKSQIKLTKSRIDVIRRKKKATLKFLKKDIADLLANGLDINAYGRAE 60
           M DG LGRGF    K  IKLTK+RIDV+RRK+ AT+KFLK+D+ADL+ NG D NA+ RA 
Sbjct: 1   MFDGFLGRGFAPKGKPLIKLTKNRIDVLRRKRNATIKFLKRDLADLIINGHDYNAFSRAG 60

Query: 61  GLLVELTISSCYDFVEQSCDTVLKHLPIMQKQRECPEECCEAIASLMFAAARFSDLPELR 120
           GLL EL      DFVEQ+CD V K L  MQK  ECPE+C EAI+SLMFAA+ FS+LPELR
Sbjct: 61  GLLDELRYLWSLDFVEQTCDFVYKQLSTMQKTPECPEDCREAISSLMFAASGFSELPELR 120

Query: 121 ELRQIFQERFGTSVEHLENRKFVENLASKPSTLEKKVQLLQDIALEFSIKWDSVGFEKR- 180
           ELRQ+F E++  S+    N++ VEN++SKP ++EKKV+L++D+ALEFSI+WDS  FEKR 
Sbjct: 121 ELRQMFHEKYTDSLALFVNQELVENMSSKPFSMEKKVKLMEDVALEFSIRWDSKDFEKRI 180

Query: 181 --------MSTPPA---------YAQGLPK--DF-GAHNAAI--EKSLSHAKEIDPRVKD 240
                   M TP +             LPK  +F G+ N      K+   ++  DP  + 
Sbjct: 181 VRQNSISVMETPKSTNDKYKPVDRNMALPKREEFEGSENGVSLNRKTAEASERRDPLFQS 240

Query: 241 D-------------GVSFKENFEQPNARHRFLNPSDSTIAGGKELKFQPKQEFPRHRHEN 300
           D             G+++KE  E  N  H   + S    A  KE     KQ   R +H+ 
Sbjct: 241 DKESYQNGLRGNQRGLTYKERSE--NVLHASRSESKDNKAERKEFYLHSKQNPAREKHQ- 300

Query: 301 RVYDEEETILKSDGRINLYGEKKG---STVPKHE------ARIGRMGSSSSSE------- 360
            +++E +TI+      NL G+  G     V  H+      +      S S  E       
Sbjct: 301 PIFNEGDTIVMKVNYGNL-GQGNGHRPGVVDAHKKTEFVASERKEFYSQSKQEPSRERHQ 360

Query: 361 ---------VLKDADNGLVVQNGRERRVPDYLKSSPYNNSGLAPTSKHEAGN----GMMS 420
                    V+K      V  NG +  V D  K    N S    +S  +  +    G   
Sbjct: 361 PIFNDGDTIVMKVKHENHVQGNGHKNGVVDLHKKIEVNASEKLKSSSSKRADKLVIGFKQ 420

Query: 421 SAFGPRRMGSSSSSEVLGDVDDGTTVV-------------------HNDRERTVPNYLKS 480
            +F        +       V+D T+                     HNDRE      L  
Sbjct: 421 ESFFQGYKHEKNEEHAHQKVEDSTSRPPKPNSKSKRAESINPGSRHHNDRESKENAVL-- 480

Query: 481 PPYSKSGLAPSDIKEPSSGNTHTG---HGYAVHQGKAEEDKHQNTKPSFNSILPPPYVKA 540
                  +  S  ++PS  N   G   + +A    K EE + +  K  F   LPPPYVK 
Sbjct: 481 -------VGKSTEEDPSGDNVKGGEYEYDHANPARKVEERETERMKSPFYKSLPPPYVKK 540

Query: 541 N-SRRKDNKDRSHDNNCVSMGPQKPVKSEMITGVELEPRHSDHERQVISPLRASSHG--- 600
           + ++ +  K  + DN      P+     E       E  H D+ + V    R +  G   
Sbjct: 541 SIAKARHEKAEALDN------PKARFDGE-------EGNHPDNGKNVYGAERRNGAGHHE 600

Query: 601 -GEMDRFFGGRIPPDALPKPRSVRRRHHKPRSSHSVDDNAEDIRTVRKKSRSSSRRRDDK 660
             ++D                S++R+ +  R  H V+   +D         SS RR + +
Sbjct: 601 VNDIDN--------------ASLKRQTN--RRKHIVESGGDD-------HISSRRRENSR 660

Query: 661 RGLQLLIDEQENERDEEERIIDKLLIHYSKKPSTFEPGKVRRKPKNHLSLENGTDSAKSP 705
           +GLQ+LID  E+E+D EE+++DKLL+HYSKKPS++E              +N  + +KS 
Sbjct: 661 KGLQVLID--EDEKDSEEKMMDKLLMHYSKKPSSYEK-------------DNVQEESKSR 720

BLAST of CmaCh10G006080 vs. TAIR 10
Match: AT1G79910.1 (Regulator of Vps4 activity in the MVB pathway protein )

HSP 1 Score: 193.4 bits (490), Expect = 6.3e-49
Identity = 92/184 (50.00%), Postives = 134/184 (72.83%), Query Frame = 0

Query: 1   MLDGIL-GRGFTSNKSQIKLTKSRIDVIRRKKKATLKFLKKDIADLLANGLDINAYGRAE 60
           M DG+   + +T  KS +K+TK+R+D ++RKK +  K+LK DI DLL N LD NAYGRAE
Sbjct: 1   MFDGLFKPKFYTKCKSLVKITKTRVDTVKRKKNSVCKYLKNDIVDLLKNSLDYNAYGRAE 60

Query: 61  GLLVELTISSCYDFVEQSCDTVLKHLPIMQKQRECPEECCEAIASLMFAAARFSDLPELR 120
           GL+ E    +CY+F+EQ C+ V  ++ ++QK   CP+EC EAI+SL++AAAR S++PELR
Sbjct: 61  GLIEEKRRLACYEFLEQFCNCVASNVSLLQKSIRCPDECREAISSLVYAAARVSEVPELR 120

Query: 121 ELRQIFQERFGTSVEHLENRKFVENLASKPSTLEKKVQLLQDIALEFSIKWDSVGFEKRM 180
           +LR +F ER+G +++   N +FVE   ++P + E KV+LLQ+IA E+SIKWD+   E+R+
Sbjct: 121 DLRSLFAERYGNTLDQFVNPEFVERFKAEPPSKEMKVELLQEIAREYSIKWDAKSLEQRL 180

Query: 181 STPP 184
            TPP
Sbjct: 181 YTPP 184

BLAST of CmaCh10G006080 vs. TAIR 10
Match: AT1G52315.1 (Regulator of Vps4 activity in the MVB pathway protein )

HSP 1 Score: 146.0 bits (367), Expect = 1.2e-34
Identity = 75/163 (46.01%), Postives = 107/163 (65.64%), Query Frame = 0

Query: 10  FTSNKSQIKLTKSRIDVIRRKKKATLKFLKKDIADLLANGLDINAYGRAEGLLVELTISS 69
           +  +KS     K RID++RRK+ A ++  K DI + L NG D  AY RAE LL EL I S
Sbjct: 11  YKKSKSTTSYMKIRIDIVRRKRIAMVRNYKTDIVNFLKNGQDSEAYRRAELLLEELRIIS 70

Query: 70  CYDFVEQSCDTVLKHLPIMQKQRECPEECCEAIASLMFAAARFSDLPELRELRQIFQERF 129
           CYD +E+ CD + ++L +M K+RECPEEC EA++SL++A A   D+PEL++LR +F +RF
Sbjct: 71  CYDLIERFCDCISENLSLMLKKRECPEECREAVSSLIYATAWVPDVPELKDLRAVFTKRF 130

Query: 130 GTSVEHLENRKFVENL-ASKPSTLEKKVQLLQDIALEFSIKWD 172
           G  +    N + VE     +P + E K+Q ++D+A EFSI WD
Sbjct: 131 GNFIASSVNHELVEKTELLRPPSRELKIQTVKDVANEFSINWD 173

BLAST of CmaCh10G006080 vs. TAIR 10
Match: AT1G79910.2 (Regulator of Vps4 activity in the MVB pathway protein )

HSP 1 Score: 134.0 bits (336), Expect = 4.6e-31
Identity = 61/125 (48.80%), Postives = 92/125 (73.60%), Query Frame = 0

Query: 59  EGLLVELTISSCYDFVEQSCDTVLKHLPIMQKQRECPEECCEAIASLMFAAARFSDLPEL 118
           EGL+ E    +CY+F+EQ C+ V  ++ ++QK   CP+EC EAI+SL++AAAR S++PEL
Sbjct: 46  EGLIEEKRRLACYEFLEQFCNCVASNVSLLQKSIRCPDECREAISSLVYAAARVSEVPEL 105

Query: 119 RELRQIFQERFGTSVEHLENRKFVENLASKPSTLEKKVQLLQDIALEFSIKWDSVGFEKR 178
           R+LR +F ER+G +++   N +FVE   ++P + E KV+LLQ+IA E+SIKWD+   E+R
Sbjct: 106 RDLRSLFAERYGNTLDQFVNPEFVERFKAEPPSKEMKVELLQEIAREYSIKWDAKSLEQR 165

Query: 179 MSTPP 184
           + TPP
Sbjct: 166 LYTPP 170

BLAST of CmaCh10G006080 vs. TAIR 10
Match: AT4G35730.1 (Regulator of Vps4 activity in the MVB pathway protein )

HSP 1 Score: 113.6 bits (283), Expect = 6.4e-25
Identity = 69/216 (31.94%), Postives = 122/216 (56.48%), Query Frame = 0

Query: 8   RGFTSN--KSQIKLTKSRIDVIRRKKKATLKFLKKDIADLLANGLDINAYGRAEGLLVEL 67
           RGF S+  K+  K+  +RI +IR K+   +K +++DIA LL +G D  A  R E ++ E 
Sbjct: 24  RGFNSSKCKTAAKMAVARIKLIRNKRLVVVKQMRRDIAVLLQSGQDATARIRVEHVIREQ 83

Query: 68  TISSCYDFVEQSCDTVLKHLPIMQKQRECPEECCEAIASLMFAAARFSDLPELRELRQIF 127
            I +  + +E  C+ ++  L I+ KQ++CP +  E IASL+FAA R S++PEL +LR IF
Sbjct: 84  NIQAANEIIELFCELIVSRLTIITKQKQCPVDLKEGIASLIFAAPRCSEIPELGDLRDIF 143

Query: 128 QERFG----TSVEHLE-----NRKFVENLASKPSTLEKKVQLLQDIALEFSIKWDSVGFE 187
            +++G    ++   L      NR  ++ L+ +    E K++++++IA EF + WD+   E
Sbjct: 144 AKKYGKDFVSAATDLRPSCGVNRMLIDKLSVRNPGGEYKLKIMKEIAKEFQVDWDTTETE 203

Query: 188 KRMSTPPAYAQGLPKDFGAHNAAIEKSLSHAKEIDP 213
           + +  P   +   P+ F + ++      +  + IDP
Sbjct: 204 QELLKPQEESIDGPRKFVSASSLPVNRAAINEPIDP 239

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q54I392.4e-0824.85IST1-like protein OS=Dictyostelium discoideum OX=44689 GN=DDB_G0289029 PE=3 SV=1[more]
P539903.8e-0628.81IST1 homolog OS=Homo sapiens OX=9606 GN=IST1 PE=1 SV=1[more]
Q9CX003.8e-0628.81IST1 homolog OS=Mus musculus OX=10090 GN=Ist1 PE=1 SV=1[more]
Q5R6G83.8e-0628.81IST1 homolog OS=Pongo abelii OX=9601 GN=IST1 PE=2 SV=1[more]
Q568Z63.8e-0628.81IST1 homolog OS=Rattus norvegicus OX=10116 GN=Ist1 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1JEU20.0e+0099.72uncharacterized protein LOC111485130 OS=Cucurbita maxima OX=3661 GN=LOC111485130... [more]
A0A6J1HAK90.0e+0094.04uncharacterized protein LOC111461604 OS=Cucurbita moschata OX=3662 GN=LOC1114616... [more]
A0A5A7TSR70.0e+0078.78IST1 like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold114G001320 PE=... [more]
A0A1S3CKP30.0e+0078.78uncharacterized protein LOC103501548 isoform X1 OS=Cucumis melo OX=3656 GN=LOC10... [more]
A0A5D3BK142.6e-31078.78IST1 like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold596G00590 PE=3... [more]
Match NameE-valueIdentityDescription
XP_022987631.10.0e+0099.72uncharacterized protein LOC111485130 [Cucurbita maxima][more]
KAG6589906.10.0e+0095.46IST1-like protein, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_023516086.10.0e+0094.61uncharacterized protein LOC111780056 [Cucurbita pepo subsp. pepo][more]
XP_022960960.10.0e+0094.04uncharacterized protein LOC111461604 [Cucurbita moschata][more]
KAG7023576.10.0e+0093.90IST1-like protein [Cucurbita argyrosperma subsp. argyrosperma][more]
Match NameE-valueIdentityDescription
AT4G32350.15.1e-9135.51Regulator of Vps4 activity in the MVB pathway protein [more]
AT1G79910.16.3e-4950.00Regulator of Vps4 activity in the MVB pathway protein [more]
AT1G52315.11.2e-3446.01Regulator of Vps4 activity in the MVB pathway protein [more]
AT1G79910.24.6e-3148.80Regulator of Vps4 activity in the MVB pathway protein [more]
AT4G35730.16.4e-2531.94Regulator of Vps4 activity in the MVB pathway protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 567..594
NoneNo IPR availableCOILSCoilCoilcoord: 28..48
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 328..566
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 484..500
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 359..373
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 406..420
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 331..350
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 290..309
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 628..642
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 225..244
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 600..664
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 539..555
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 601..616
NoneNo IPR availablePANTHERPTHR12161:SF14REGULATOR OF VPS4 ACTIVITY IN THE MVB PATHWAY PROTEINcoord: 12..704
IPR005061Vacuolar protein sorting-associated protein Ist1PFAMPF03398Ist1coord: 14..168
e-value: 4.4E-42
score: 143.8
IPR005061Vacuolar protein sorting-associated protein Ist1PANTHERPTHR12161IST1 FAMILY MEMBERcoord: 12..704
IPR042277Vacuolar protein sorting-associated protein IST1-likeGENE3D1.20.1260.60coord: 4..178
e-value: 2.5E-47
score: 162.8

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh10G006080.1CmaCh10G006080.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0015031 protein transport