CmaCh10G004030 (gene) Cucurbita maxima (Rimu) v1.1

Overview


Name	CmaCh10G004030
Type	gene
Organism	Cucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Description	nicotianamine synthase-like
Location	Cma_Chr10: 1871401 .. 1872258 (+)
RNA-Seq Expression	CmaCh10G004030
Synteny	CmaCh10G004030

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAAGCCCTCCAGATTCTTGGCACCGAAACCGATATCTCCGAGGAGGTTTTGATAGCTCGAGTCATCGACCTCCATGGGAGCATCTCGAGGCTTGAAACCCTAAAGCCATGCAAGGAAGTGAACGGCCTCTTCACCAACCTTGTGAAATTTTGCATTTTGCCTTGTTCAATAGATGTCTCCACATTGCCACCCCATTTACAAGCCATTCGTGAGAGCCTCATACTTTTTTGTGGCCGAGCCGAGGGGTTACTCGAGTTAGAATTCTCGACCATGCTCGGAAAAATCCCAAAACCTCTCAACAATCTCACTCTATTTCCATATTATGACAACTACATCAAATTAGCCCACCTTGAATACAAGATATTGATTGACAATGGAGTTGTGAATCCAAAAAAGGTAGCTTTTGTGGGGTCCGGTCCTCTTCCTCTAACTTCCATTATATTGGCCATGCAACACATGAAGAACACGTATTTCGACAACTACGACATCGATAGCATGGCAAATGAGGTGGCGGGAAAGATCGTGGCTTCCGACGGTGAGCTGGAGGGAAGAATGAAGTTTTGGACGAGGGATATTGTTGAGGTAGGAGAAGAGCTTGAGGGATACGATTGTGTGTTCTTGGCGGCTCTTGTGGGGTTGAAGAAGGAAGATAAAGTGAAGATTTTAAGTCACTTGAGAAAGTATATGAAACAAGGTGGGGTTTTGGTTGTGAGAAGTGCTAAAGGAGGTCGAGCCTTTTTGTACCCCGTGGTTGAGGTCGCCGATATGGTTGGCTTTGACATTCTCTCTATTTTTCATCCCTCTGATGACGTCGTCAATTCCGTTCTCTTGGCTCGCAAACCTATACTCATATGA

mRNA sequence

ATGGAAGCCCTCCAGATTCTTGGCACCGAAACCGATATCTCCGAGGAGGTTTTGATAGCTCGAGTCATCGACCTCCATGGGAGCATCTCGAGGCTTGAAACCCTAAAGCCATGCAAGGAAGTGAACGGCCTCTTCACCAACCTTGTGAAATTTTGCATTTTGCCTTGTTCAATAGATGTCTCCACATTGCCACCCCATTTACAAGCCATTCGTGAGAGCCTCATACTTTTTTGTGGCCGAGCCGAGGGGTTACTCGAGTTAGAATTCTCGACCATGCTCGGAAAAATCCCAAAACCTCTCAACAATCTCACTCTATTTCCATATTATGACAACTACATCAAATTAGCCCACCTTGAATACAAGATATTGATTGACAATGGAGTTGTGAATCCAAAAAAGGTAGCTTTTGTGGGGTCCGGTCCTCTTCCTCTAACTTCCATTATATTGGCCATGCAACACATGAAGAACACGTATTTCGACAACTACGACATCGATAGCATGGCAAATGAGGTGGCGGGAAAGATCGTGGCTTCCGACGGTGAGCTGGAGGGAAGAATGAAGTTTTGGACGAGGGATATTGTTGAGGTAGGAGAAGAGCTTGAGGGATACGATTGTGTGTTCTTGGCGGCTCTTGTGGGGTTGAAGAAGGAAGATAAAGTGAAGATTTTAAGTCACTTGAGAAAGTATATGAAACAAGGTGGGGTTTTGGTTGTGAGAAGTGCTAAAGGAGGTCGAGCCTTTTTGTACCCCGTGGTTGAGGTCGCCGATATGGTTGGCTTTGACATTCTCTCTATTTTTCATCCCTCTGATGACGTCGTCAATTCCGTTCTCTTGGCTCGCAAACCTATACTCATATGA

Coding sequence (CDS)

ATGGAAGCCCTCCAGATTCTTGGCACCGAAACCGATATCTCCGAGGAGGTTTTGATAGCTCGAGTCATCGACCTCCATGGGAGCATCTCGAGGCTTGAAACCCTAAAGCCATGCAAGGAAGTGAACGGCCTCTTCACCAACCTTGTGAAATTTTGCATTTTGCCTTGTTCAATAGATGTCTCCACATTGCCACCCCATTTACAAGCCATTCGTGAGAGCCTCATACTTTTTTGTGGCCGAGCCGAGGGGTTACTCGAGTTAGAATTCTCGACCATGCTCGGAAAAATCCCAAAACCTCTCAACAATCTCACTCTATTTCCATATTATGACAACTACATCAAATTAGCCCACCTTGAATACAAGATATTGATTGACAATGGAGTTGTGAATCCAAAAAAGGTAGCTTTTGTGGGGTCCGGTCCTCTTCCTCTAACTTCCATTATATTGGCCATGCAACACATGAAGAACACGTATTTCGACAACTACGACATCGATAGCATGGCAAATGAGGTGGCGGGAAAGATCGTGGCTTCCGACGGTGAGCTGGAGGGAAGAATGAAGTTTTGGACGAGGGATATTGTTGAGGTAGGAGAAGAGCTTGAGGGATACGATTGTGTGTTCTTGGCGGCTCTTGTGGGGTTGAAGAAGGAAGATAAAGTGAAGATTTTAAGTCACTTGAGAAAGTATATGAAACAAGGTGGGGTTTTGGTTGTGAGAAGTGCTAAAGGAGGTCGAGCCTTTTTGTACCCCGTGGTTGAGGTCGCCGATATGGTTGGCTTTGACATTCTCTCTATTTTTCATCCCTCTGATGACGTCGTCAATTCCGTTCTCTTGGCTCGCAAACCTATACTCATATGA

Protein sequence

MEALQILGTETDISEEVLIARVIDLHGSISRLETLKPCKEVNGLFTNLVKFCILPCSIDVSTLPPHLQAIRESLILFCGRAEGLLELEFSTMLGKIPKPLNNLTLFPYYDNYIKLAHLEYKILIDNGVVNPKKVAFVGSGPLPLTSIILAMQHMKNTYFDNYDIDSMANEVAGKIVASDGELEGRMKFWTRDIVEVGEELEGYDCVFLAALVGLKKEDKVKILSHLRKYMKQGGVLVVRSAKGGRAFLYPVVEVADMVGFDILSIFHPSDDVVNSVLLARKPILI

Homology

[Close]

BLAST of CmaCh10G004030 vs. ExPASy Swiss-Prot
Match: Q9XGI7 (Nicotianamine synthase OS=Solanum lycopersicum OX=4081 GN=CHLN PE=2 SV=1)

HSP 1 Score: 294.7 bits (753), Expect = 1.2e-78
Identity = 145/264 (54.92%), Postives = 192/264 (72.73%), Query Frame = 0


Query: 18  LIARVIDLHGSISRLETLKPCKEVNGLFTNLVKFCILPCSIDVSTLPPHLQAIRESLILF 77
           ++ +V +L+  ISRLE L P K+VN LFT+LV  C+ P  IDVS L   +Q IR  LI  
Sbjct: 9   VVEKVCELYEQISRLENLSPSKDVNVLFTDLVHTCMPPNPIDVSKLCQKIQEIRSHLIKL 68

Query: 78  CGRAEGLLELEFSTMLGKIPKPLNNLTLFPYYDNYIKLAHLEYKILIDNGVVNPKKVAFV 137
           CG+AEGLLE  FS +L     PL +L +FPY+DNYIKL+ LEY IL  N    PKK+AF+
Sbjct: 69  CGQAEGLLESHFSKILSSYENPLQHLHIFPYFDNYIKLSLLEYNILTKNTTNIPKKIAFI 128

Query: 138 GSGPLPLTSIILAMQHMKNTYFDNYDIDSMANEVAGKIVASDGELEGRMKFWTRDIVEVG 197
           GSGPLPLTS++LA +H+K T F NYDID  AN +A  +VA+D ++  RM F T D+++V 
Sbjct: 129 GSGPLPLTSLVLATKHLKTTCFHNYDIDVDANFMASALVAADPDMSSRMTFHTADVMDVT 188

Query: 198 EELEGYDCVFLAALVGLKKEDKVKILSHLRKYMKQGGVLVVRSAKGGRAFLYPVVEVADM 257
             L+ YD VFLAALVG+ KEDKVK++ HL KYM  G  L++RSA G RAFLYPV++  D+
Sbjct: 189 CALKDYDVVFLAALVGMDKEDKVKVVDHLAKYMSPGATLMLRSAHGARAFLYPVLDPRDL 248

Query: 258 VGFDILSIFHPSDDVVNSVLLARK 282
            GF++LS++HP+D+V+NSV++ARK
Sbjct: 249 RGFEVLSVYHPTDEVINSVIIARK 272

[Close]

BLAST of CmaCh10G004030 vs. ExPASy Swiss-Prot
Match: Q9C7X5 (Probable nicotianamine synthase 4 OS=Arabidopsis thaliana OX=3702 GN=NAS4 PE=2 SV=1)

HSP 1 Score: 281.2 bits (718), Expect = 1.3e-74
Identity = 139/271 (51.29%), Postives = 203/271 (74.91%), Query Frame = 0


Query: 15  EEVLIARVIDLHGSISRLETLKPCKEVNGLFTNLVKFCILP-CSIDVSTLPPHLQAIRES 74
           ++ L+ ++ DL+  IS+LETLKPC++V+ LF  LV  CI P  +IDV+ +  ++Q +R +
Sbjct: 6   DDQLVNKICDLYEKISKLETLKPCEDVDTLFKQLVSTCIPPNPNIDVTKMSENIQEMRSN 65

Query: 75  LILFCGRAEGLLELEFSTMLGKI-PKPLNNLTLFPYYDNYIKLAHLEYKILID--NGVVN 134
           LI  CG AEG LE  FS++L      PL++L LFPYY+NY+KL+ LE+ +L    NG V 
Sbjct: 66  LIKICGEAEGYLEHHFSSILTSFEDNPLHHLNLFPYYNNYLKLSKLEFDLLEQNLNGFV- 125

Query: 135 PKKVAFVGSGPLPLTSIILAMQHMKNTYFDNYDIDSMANEVAGKIVASDGELEGRMKFWT 194
           P+ VAF+GSGPLPLTS++LA  H+K++ F N+DID  AN VA ++V+SD +L  RM F T
Sbjct: 126 PRTVAFIGSGPLPLTSVVLASSHLKDSIFHNFDIDPSANMVAARLVSSDPDLSQRMFFHT 185

Query: 195 RDIVEVGEELEGYDCVFLAALVGLKKEDKVKILSHLRKYMKQGGVLVVRSAKGGRAFLYP 254
            DI++V E L+G+D VFLAALVG+ K++KVK++ HL K+M  G +L++RSA G RAFLYP
Sbjct: 186 VDIMDVTESLKGFDVVFLAALVGMDKKEKVKVVEHLEKHMSPGALLMLRSAHGPRAFLYP 245

Query: 255 VVEVADMVGFDILSIFHPSDDVVNSVLLARK 282
           +VE  D+ GF++LS++HP+D+V+NS++++RK
Sbjct: 246 IVEPCDLEGFEVLSVYHPTDEVINSIVISRK 275

[Close]

BLAST of CmaCh10G004030 vs. ExPASy Swiss-Prot
Match: O80483 (Nicotianamine synthase 3 OS=Arabidopsis thaliana OX=3702 GN=NAS3 PE=2 SV=1)

HSP 1 Score: 274.6 bits (701), Expect = 1.2e-72
Identity = 143/271 (52.77%), Postives = 198/271 (73.06%), Query Frame = 0


Query: 15  EEVLIARVIDLHGSISRLETLKPCKEVNGLFTNLVKFCILP-CSIDVSTLPPHLQAIRES 74
           +E L+  + DL+  IS+LE+LKP ++VN LF  LV  CI P  +IDV+ +   +Q IR +
Sbjct: 5   DEQLVQTICDLYEKISKLESLKPSEDVNILFKQLVSTCIPPNPNIDVTKMCDRVQEIRLN 64

Query: 75  LILFCGRAEGLLELEFSTMLGKI-PKPLNNLTLFPYYDNYIKLAHLEYKILID--NGVVN 134
           LI  CG AEG LE  FS++L      PL++L +FPYY+NY+KL  LE+ +L    NG V 
Sbjct: 65  LIKICGLAEGHLENHFSSILTSYQDNPLHHLNIFPYYNNYLKLGKLEFDLLEQNLNGFV- 124

Query: 135 PKKVAFVGSGPLPLTSIILAMQHMKNTYFDNYDIDSMANEVAGKIVASDGELEGRMKFWT 194
           PK VAF+GSGPLPLTSI+LA  H+K+T F N+DID  AN +A  +V+SD ++  RM F T
Sbjct: 125 PKSVAFIGSGPLPLTSIVLASFHLKDTIFHNFDIDPSANSLASLLVSSDPDISQRMFFHT 184

Query: 195 RDIVEVGEELEGYDCVFLAALVGLKKEDKVKILSHLRKYMKQGGVLVVRSAKGGRAFLYP 254
            DI++V E L+ +D VFLAALVG+ KE+KVK++ HL+K+M  G VL++RSA G RAFLYP
Sbjct: 185 VDIMDVTESLKSFDVVFLAALVGMNKEEKVKVIEHLQKHMAPGAVLMLRSAHGPRAFLYP 244

Query: 255 VVEVADMVGFDILSIFHPSDDVVNSVLLARK 282
           +VE  D+ GF++LSI+HP+DDV+NSV++++K
Sbjct: 245 IVEPCDLQGFEVLSIYHPTDDVINSVVISKK 274

[Close]

BLAST of CmaCh10G004030 vs. ExPASy Swiss-Prot
Match: Q9FF79 (Nicotianamine synthase 1 OS=Arabidopsis thaliana OX=3702 GN=NAS1 PE=2 SV=1)

HSP 1 Score: 267.3 bits (682), Expect = 2.0e-70
Identity = 135/269 (50.19%), Postives = 190/269 (70.63%), Query Frame = 0


Query: 17  VLIARVIDLHGSISRLETLKPCKEVNGLFTNLVKFCI-LPCSIDVSTLPPHLQAIRESLI 76
           +++ ++IDL+  IS+L++LKP K V+ LF  LV  C+    +IDV+ +   ++ +R +LI
Sbjct: 7   LVVKQIIDLYDQISKLKSLKPSKNVDTLFGQLVSTCLPTDTNIDVTNMCEEVKDMRANLI 66

Query: 77  LFCGRAEGLLELEFSTMLGKIPK---PLNNLTLFPYYDNYIKLAHLEYKILIDNGVVNPK 136
             CG AEG LE  FST+LG + +   PL++L +FPYY NY+KL  LE+ +L  +    P 
Sbjct: 67  KLCGEAEGYLEQHFSTILGSLQEDQNPLDHLHIFPYYSNYLKLGKLEFDLLSQHSSHVPT 126

Query: 137 KVAFVGSGPLPLTSIILAMQHMKNTYFDNYDIDSMANEVAGKIVASDGELEGRMKFWTRD 196
           K+AFVGSGP+PLTSI+LA  H+ NT F N+DIDS AN +A  +V+ D +L  RM F T D
Sbjct: 127 KIAFVGSGPMPLTSIVLAKFHLPNTTFHNFDIDSHANTLASNLVSRDPDLSKRMIFHTTD 186

Query: 197 IVEVGEELEGYDCVFLAALVGLKKEDKVKILSHLRKYMKQGGVLVVRSAKGGRAFLYPVV 256
           ++   E L+ YD VFLAALVG+ KE KVK + HL K+M  G VL++RSA   RAFLYP+V
Sbjct: 187 VLNATEGLDQYDVVFLAALVGMDKESKVKAIEHLEKHMAPGAVLMLRSAHALRAFLYPIV 246

Query: 257 EVADMVGFDILSIFHPSDDVVNSVLLARK 282
           + +D+ GF +L+I+HP+DDVVNSV++ARK
Sbjct: 247 DSSDLKGFQLLTIYHPTDDVVNSVVIARK 275

[Close]

BLAST of CmaCh10G004030 vs. ExPASy Swiss-Prot
Match: Q9FKT9 (Nicotianamine synthase 2 OS=Arabidopsis thaliana OX=3702 GN=NAS2 PE=2 SV=1)

HSP 1 Score: 266.9 bits (681), Expect = 2.6e-70
Identity = 136/269 (50.56%), Postives = 185/269 (68.77%), Query Frame = 0


Query: 17  VLIARVIDLHGSISRLETLKPCKEVNGLFTNLVKFCILPCSIDVSTLPPH---LQAIRES 76
           +++ +++DL+  IS LE+LKP K V+ LF  LV  C LP   ++     H   ++ +R  
Sbjct: 7   LVVKQIMDLYNQISNLESLKPSKNVDTLFRQLVSTC-LPTDTNIDVTEIHDEKVKDMRSH 66

Query: 77  LILFCGRAEGLLELEFSTMLGKI-PKPLNNLTLFPYYDNYIKLAHLEYKILIDNGVVNPK 136
           LI  CG AEG LE  FS +LG     PLN+L +FPYY+NY+KL  LE+ +L  +    P 
Sbjct: 67  LIKLCGEAEGYLEQHFSAILGSFEDNPLNHLHIFPYYNNYLKLGKLEFDLLSQHTTHVPT 126

Query: 137 KVAFVGSGPLPLTSIILAMQHMKNTYFDNYDIDSMANEVAGKIVASDGELEGRMKFWTRD 196
           KVAF+GSGP+PLTSI+LA  H+ NT F N+DIDS AN +A  +V+ D +L  RM F T D
Sbjct: 127 KVAFIGSGPMPLTSIVLAKFHLPNTTFHNFDIDSHANTLASNLVSRDSDLSKRMIFHTTD 186

Query: 197 IVEVGEELEGYDCVFLAALVGLKKEDKVKILSHLRKYMKQGGVLVVRSAKGGRAFLYPVV 256
           ++   E L+ YD VFLAALVG+ KE KVK + HL K+M  G V+++RSA G RAFLYP+V
Sbjct: 187 VLNAKEGLDQYDVVFLAALVGMDKESKVKAIEHLEKHMAPGAVVMLRSAHGLRAFLYPIV 246

Query: 257 EVADMVGFDILSIFHPSDDVVNSVLLARK 282
           +  D+ GF++L+I+HPSDDVVNSV++ARK
Sbjct: 247 DSCDLKGFEVLTIYHPSDDVVNSVVIARK 274

[Close]

BLAST of CmaCh10G004030 vs. ExPASy TrEMBL
Match: A0A6J1JBQ8 (nicotianamine synthase-like OS=Cucurbita maxima OX=3661 GN=LOC111485338 PE=3 SV=1)

HSP 1 Score: 565.5 bits (1456), Expect = 1.3e-157
Identity = 285/285 (100.00%), Postives = 285/285 (100.00%), Query Frame = 0


Query: 1   MEALQILGTETDISEEVLIARVIDLHGSISRLETLKPCKEVNGLFTNLVKFCILPCSIDV 60
           MEALQILGTETDISEEVLIARVIDLHGSISRLETLKPCKEVNGLFTNLVKFCILPCSIDV
Sbjct: 1   MEALQILGTETDISEEVLIARVIDLHGSISRLETLKPCKEVNGLFTNLVKFCILPCSIDV 60

Query: 61  STLPPHLQAIRESLILFCGRAEGLLELEFSTMLGKIPKPLNNLTLFPYYDNYIKLAHLEY 120
           STLPPHLQAIRESLILFCGRAEGLLELEFSTMLGKIPKPLNNLTLFPYYDNYIKLAHLEY
Sbjct: 61  STLPPHLQAIRESLILFCGRAEGLLELEFSTMLGKIPKPLNNLTLFPYYDNYIKLAHLEY 120

Query: 121 KILIDNGVVNPKKVAFVGSGPLPLTSIILAMQHMKNTYFDNYDIDSMANEVAGKIVASDG 180
           KILIDNGVVNPKKVAFVGSGPLPLTSIILAMQHMKNTYFDNYDIDSMANEVAGKIVASDG
Sbjct: 121 KILIDNGVVNPKKVAFVGSGPLPLTSIILAMQHMKNTYFDNYDIDSMANEVAGKIVASDG 180

Query: 181 ELEGRMKFWTRDIVEVGEELEGYDCVFLAALVGLKKEDKVKILSHLRKYMKQGGVLVVRS 240
           ELEGRMKFWTRDIVEVGEELEGYDCVFLAALVGLKKEDKVKILSHLRKYMKQGGVLVVRS
Sbjct: 181 ELEGRMKFWTRDIVEVGEELEGYDCVFLAALVGLKKEDKVKILSHLRKYMKQGGVLVVRS 240

Query: 241 AKGGRAFLYPVVEVADMVGFDILSIFHPSDDVVNSVLLARKPILI 286
           AKGGRAFLYPVVEVADMVGFDILSIFHPSDDVVNSVLLARKPILI
Sbjct: 241 AKGGRAFLYPVVEVADMVGFDILSIFHPSDDVVNSVLLARKPILI 285

[Close]

BLAST of CmaCh10G004030 vs. ExPASy TrEMBL
Match: A0A6J1E292 (nicotianamine synthase-like OS=Cucurbita moschata OX=3662 GN=LOC111430119 PE=3 SV=1)

HSP 1 Score: 543.9 bits (1400), Expect = 4.0e-151
Identity = 274/285 (96.14%), Postives = 277/285 (97.19%), Query Frame = 0


Query: 1   MEALQILGTETDISEEVLIARVIDLHGSISRLETLKPCKEVNGLFTNLVKFCILPCSIDV 60
           M AL ILGTETDISEE LIARVIDLHGSISRLETLKPCKEVNGLFT+LVK CILPCSIDV
Sbjct: 1   MAALHILGTETDISEEFLIARVIDLHGSISRLETLKPCKEVNGLFTSLVKLCILPCSIDV 60

Query: 61  STLPPHLQAIRESLILFCGRAEGLLELEFSTMLGKIPKPLNNLTLFPYYDNYIKLAHLEY 120
           STL PHLQAIRESLIL CGRAEGLLELEFSTMLGKIPKPLNNLTLFPYY NYIKLAHLEY
Sbjct: 61  STLAPHLQAIRESLILLCGRAEGLLELEFSTMLGKIPKPLNNLTLFPYYGNYIKLAHLEY 120

Query: 121 KILIDNGVVNPKKVAFVGSGPLPLTSIILAMQHMKNTYFDNYDIDSMANEVAGKIVASDG 180
           KILIDNGVVNPKKVAFVGSGPLPLTSIILAMQHMKNTYFDNYD+DSMANEVAGKIVASDG
Sbjct: 121 KILIDNGVVNPKKVAFVGSGPLPLTSIILAMQHMKNTYFDNYDVDSMANEVAGKIVASDG 180

Query: 181 ELEGRMKFWTRDIVEVGEELEGYDCVFLAALVGLKKEDKVKILSHLRKYMKQGGVLVVRS 240
           ELEGRMKFWTRDIVEVGEELEGYDCVFLAALVGL KEDKVKILSHLRKYMKQGGVLVVRS
Sbjct: 181 ELEGRMKFWTRDIVEVGEELEGYDCVFLAALVGLNKEDKVKILSHLRKYMKQGGVLVVRS 240

Query: 241 AKGGRAFLYPVVEVADMVGFDILSIFHPSDDVVNSVLLARKPILI 286
           AKGGRAFLYPVVEVADMVGFDILSIFHPSDDVVNSVLLARKPIL+
Sbjct: 241 AKGGRAFLYPVVEVADMVGFDILSIFHPSDDVVNSVLLARKPILL 285

[Close]

BLAST of CmaCh10G004030 vs. ExPASy TrEMBL
Match: A0A0A0LUJ7 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G423010 PE=3 SV=1)

HSP 1 Score: 453.0 bits (1164), Expect = 9.3e-124
Identity = 222/281 (79.00%), Postives = 252/281 (89.68%), Query Frame = 0


Query: 1   MEALQILGTETDISEEVLIARVIDLHGSISRLETLKPCKEVNGLFTNLVKFCILPCSIDV 60
           M ALQI GTE +IS   L+ R+IDLH SIS+LETL+PCK+VN LFTNLV  CILPCSIDV
Sbjct: 1   MAALQIHGTEAEISAAFLVDRIIDLHESISKLETLRPCKKVNNLFTNLVTLCILPCSIDV 60

Query: 61  STLPPHLQAIRESLILFCGRAEGLLELEFSTMLGKIPKPLNNLTLFPYYDNYIKLAHLEY 120
           STLPP+LQ IRESLI+ CG+AEGLLELEFST+L KIPKPLNNLTLFPYY NYIKLA+LE 
Sbjct: 61  STLPPNLQVIRESLIILCGQAEGLLELEFSTLLSKIPKPLNNLTLFPYYQNYIKLANLEN 120

Query: 121 KILIDNGVVNPKKVAFVGSGPLPLTSIILAMQHMKNTYFDNYDIDSMANEVAGKIVASDG 180
           KIL DNG+VNPKKVAFVGSGPLPLTSII+AMQHMK T+FDNYD+DS+AN+VA KIV SD 
Sbjct: 121 KILNDNGIVNPKKVAFVGSGPLPLTSIIMAMQHMKGTHFDNYDVDSVANDVARKIVGSDS 180

Query: 181 ELEGRMKFWTRDIVEVGEELEGYDCVFLAALVGLKKEDKVKILSHLRKYMKQGGVLVVRS 240
           +LEGRMKF + DIV+V EEL GYDCVFLAALVG+ KE+KVKI+ HLRKYMK+GG+L+VRS
Sbjct: 181 DLEGRMKFCSSDIVDVKEELGGYDCVFLAALVGMNKEEKVKIIRHLRKYMKEGGILLVRS 240

Query: 241 AKGGRAFLYPVVEVADMVGFDILSIFHPSDDVVNSVLLARK 282
           AKGGRAFLYPVVEV D+VGF+ILSIFHP+DDVVNSV+L RK
Sbjct: 241 AKGGRAFLYPVVEVEDLVGFEILSIFHPTDDVVNSVILTRK 281

[Close]

BLAST of CmaCh10G004030 vs. ExPASy TrEMBL
Match: A0A5D3CIT2 (Nicotianamine synthase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold152G00680 PE=3 SV=1)

HSP 1 Score: 452.6 bits (1163), Expect = 1.2e-123
Identity = 222/281 (79.00%), Postives = 251/281 (89.32%), Query Frame = 0


Query: 1   MEALQILGTETDISEEVLIARVIDLHGSISRLETLKPCKEVNGLFTNLVKFCILPCSIDV 60
           M ALQ  G E +IS   L+ R+IDLH SI +LETL+PCK+VN LFTNLV  CILPCSIDV
Sbjct: 1   MAALQFHGMEAEISAAFLVDRIIDLHQSILKLETLRPCKKVNNLFTNLVNLCILPCSIDV 60

Query: 61  STLPPHLQAIRESLILFCGRAEGLLELEFSTMLGKIPKPLNNLTLFPYYDNYIKLAHLEY 120
           STLPP+LQAIRESLI+ CG+AEGLLELEFST+L KIPKPLNNLTLFPYY NY+KLA+LE 
Sbjct: 61  STLPPNLQAIRESLIILCGQAEGLLELEFSTLLSKIPKPLNNLTLFPYYQNYVKLANLEN 120

Query: 121 KILIDNGVVNPKKVAFVGSGPLPLTSIILAMQHMKNTYFDNYDIDSMANEVAGKIVASDG 180
           KIL DNG+VNPKKVAFVGSGPLPLTSII+AMQHMK TYFDNYD+DS+AN+VA KIV SD 
Sbjct: 121 KILNDNGIVNPKKVAFVGSGPLPLTSIIMAMQHMKGTYFDNYDVDSVANDVARKIVGSDA 180

Query: 181 ELEGRMKFWTRDIVEVGEELEGYDCVFLAALVGLKKEDKVKILSHLRKYMKQGGVLVVRS 240
           ELEGRMKF + DIVEV EEL GYDCVFLAALVG+ KE+KVKI+ HLRKYMK+GG+L+VRS
Sbjct: 181 ELEGRMKFCSSDIVEVKEELGGYDCVFLAALVGMNKEEKVKIVRHLRKYMKEGGILLVRS 240

Query: 241 AKGGRAFLYPVVEVADMVGFDILSIFHPSDDVVNSVLLARK 282
           A+GGRAFLYPVVEV D+VGF+ILSIFHP+DDVVNSV+LARK
Sbjct: 241 AQGGRAFLYPVVEVGDLVGFEILSIFHPTDDVVNSVILARK 281

[Close]

BLAST of CmaCh10G004030 vs. ExPASy TrEMBL
Match: A0A1S3BXH0 (nicotianamine synthase-like OS=Cucumis melo OX=3656 GN=LOC103494275 PE=3 SV=1)

HSP 1 Score: 452.6 bits (1163), Expect = 1.2e-123
Identity = 222/281 (79.00%), Postives = 251/281 (89.32%), Query Frame = 0


Query: 1   MEALQILGTETDISEEVLIARVIDLHGSISRLETLKPCKEVNGLFTNLVKFCILPCSIDV 60
           M ALQ  G E +IS   L+ R+IDLH SI +LETL+PCK+VN LFTNLV  CILPCSIDV
Sbjct: 1   MAALQFHGMEAEISAAFLVDRIIDLHQSILKLETLRPCKKVNNLFTNLVNLCILPCSIDV 60

Query: 61  STLPPHLQAIRESLILFCGRAEGLLELEFSTMLGKIPKPLNNLTLFPYYDNYIKLAHLEY 120
           STLPP+LQAIRESLI+ CG+AEGLLELEFST+L KIPKPLNNLTLFPYY NY+KLA+LE 
Sbjct: 61  STLPPNLQAIRESLIILCGQAEGLLELEFSTLLSKIPKPLNNLTLFPYYQNYVKLANLEN 120

Query: 121 KILIDNGVVNPKKVAFVGSGPLPLTSIILAMQHMKNTYFDNYDIDSMANEVAGKIVASDG 180
           KIL DNG+VNPKKVAFVGSGPLPLTSII+AMQHMK TYFDNYD+DS+AN+VA KIV SD 
Sbjct: 121 KILNDNGIVNPKKVAFVGSGPLPLTSIIMAMQHMKGTYFDNYDVDSVANDVARKIVGSDA 180

Query: 181 ELEGRMKFWTRDIVEVGEELEGYDCVFLAALVGLKKEDKVKILSHLRKYMKQGGVLVVRS 240
           ELEGRMKF + DIVEV EEL GYDCVFLAALVG+ KE+KVKI+ HLRKYMK+GG+L+VRS
Sbjct: 181 ELEGRMKFCSSDIVEVKEELGGYDCVFLAALVGMNKEEKVKIVRHLRKYMKEGGILLVRS 240

Query: 241 AKGGRAFLYPVVEVADMVGFDILSIFHPSDDVVNSVLLARK 282
           A+GGRAFLYPVVEV D+VGF+ILSIFHP+DDVVNSV+LARK
Sbjct: 241 AQGGRAFLYPVVEVGDLVGFEILSIFHPTDDVVNSVILARK 281

[Close]

BLAST of CmaCh10G004030 vs. NCBI nr
Match: XP_022987937.1 (nicotianamine synthase-like [Cucurbita maxima])

HSP 1 Score: 565.5 bits (1456), Expect = 2.7e-157
Identity = 285/285 (100.00%), Postives = 285/285 (100.00%), Query Frame = 0


Query: 1   MEALQILGTETDISEEVLIARVIDLHGSISRLETLKPCKEVNGLFTNLVKFCILPCSIDV 60
           MEALQILGTETDISEEVLIARVIDLHGSISRLETLKPCKEVNGLFTNLVKFCILPCSIDV
Sbjct: 1   MEALQILGTETDISEEVLIARVIDLHGSISRLETLKPCKEVNGLFTNLVKFCILPCSIDV 60

Query: 61  STLPPHLQAIRESLILFCGRAEGLLELEFSTMLGKIPKPLNNLTLFPYYDNYIKLAHLEY 120
           STLPPHLQAIRESLILFCGRAEGLLELEFSTMLGKIPKPLNNLTLFPYYDNYIKLAHLEY
Sbjct: 61  STLPPHLQAIRESLILFCGRAEGLLELEFSTMLGKIPKPLNNLTLFPYYDNYIKLAHLEY 120

Query: 121 KILIDNGVVNPKKVAFVGSGPLPLTSIILAMQHMKNTYFDNYDIDSMANEVAGKIVASDG 180
           KILIDNGVVNPKKVAFVGSGPLPLTSIILAMQHMKNTYFDNYDIDSMANEVAGKIVASDG
Sbjct: 121 KILIDNGVVNPKKVAFVGSGPLPLTSIILAMQHMKNTYFDNYDIDSMANEVAGKIVASDG 180

Query: 181 ELEGRMKFWTRDIVEVGEELEGYDCVFLAALVGLKKEDKVKILSHLRKYMKQGGVLVVRS 240
           ELEGRMKFWTRDIVEVGEELEGYDCVFLAALVGLKKEDKVKILSHLRKYMKQGGVLVVRS
Sbjct: 181 ELEGRMKFWTRDIVEVGEELEGYDCVFLAALVGLKKEDKVKILSHLRKYMKQGGVLVVRS 240

Query: 241 AKGGRAFLYPVVEVADMVGFDILSIFHPSDDVVNSVLLARKPILI 286
           AKGGRAFLYPVVEVADMVGFDILSIFHPSDDVVNSVLLARKPILI
Sbjct: 241 AKGGRAFLYPVVEVADMVGFDILSIFHPSDDVVNSVLLARKPILI 285

[Close]

BLAST of CmaCh10G004030 vs. NCBI nr
Match: KAG6589715.1 (Nicotianamine synthase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 549.3 bits (1414), Expect = 2.0e-152
Identity = 276/285 (96.84%), Postives = 279/285 (97.89%), Query Frame = 0


Query: 1   MEALQILGTETDISEEVLIARVIDLHGSISRLETLKPCKEVNGLFTNLVKFCILPCSIDV 60
           M ALQILGTETDISEE LIARVIDLHGSISRLETLKPCKEVNGLFTNLVK CILPCSIDV
Sbjct: 1   MAALQILGTETDISEEFLIARVIDLHGSISRLETLKPCKEVNGLFTNLVKLCILPCSIDV 60

Query: 61  STLPPHLQAIRESLILFCGRAEGLLELEFSTMLGKIPKPLNNLTLFPYYDNYIKLAHLEY 120
           STL PHLQAIRESLIL CGRAEGLLELEFSTMLGKIPKPLNNLTLFPYYDNYIKLAHLEY
Sbjct: 61  STLAPHLQAIRESLILLCGRAEGLLELEFSTMLGKIPKPLNNLTLFPYYDNYIKLAHLEY 120

Query: 121 KILIDNGVVNPKKVAFVGSGPLPLTSIILAMQHMKNTYFDNYDIDSMANEVAGKIVASDG 180
           KILIDNGVVNPKKVAFVGSGPLPLTSIILAMQHMKNTYFDNYD+DSMANEVAGKIVASDG
Sbjct: 121 KILIDNGVVNPKKVAFVGSGPLPLTSIILAMQHMKNTYFDNYDVDSMANEVAGKIVASDG 180

Query: 181 ELEGRMKFWTRDIVEVGEELEGYDCVFLAALVGLKKEDKVKILSHLRKYMKQGGVLVVRS 240
           ELEGRMKFWTRDIVEVGEELEGYDCVFLAALVGL KEDKVKILSHLRKYMKQGGVLVVRS
Sbjct: 181 ELEGRMKFWTRDIVEVGEELEGYDCVFLAALVGLNKEDKVKILSHLRKYMKQGGVLVVRS 240

Query: 241 AKGGRAFLYPVVEVADMVGFDILSIFHPSDDVVNSVLLARKPILI 286
           A+GGRAFLYPVVEVADMVGFDILSIFHPSDDVVNSVLLARKPIL+
Sbjct: 241 ARGGRAFLYPVVEVADMVGFDILSIFHPSDDVVNSVLLARKPILL 285

[Close]

BLAST of CmaCh10G004030 vs. NCBI nr
Match: KAG7023395.1 (Nicotianamine synthase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 547.4 bits (1409), Expect = 7.5e-152
Identity = 275/285 (96.49%), Postives = 278/285 (97.54%), Query Frame = 0


Query: 1   MEALQILGTETDISEEVLIARVIDLHGSISRLETLKPCKEVNGLFTNLVKFCILPCSIDV 60
           M AL ILGTETDISEE LIARVIDLHGSISRLETLKPCKEVNGLFTNLVK CILPCSIDV
Sbjct: 1   MTALHILGTETDISEEFLIARVIDLHGSISRLETLKPCKEVNGLFTNLVKLCILPCSIDV 60

Query: 61  STLPPHLQAIRESLILFCGRAEGLLELEFSTMLGKIPKPLNNLTLFPYYDNYIKLAHLEY 120
           STL PHLQAIRESLIL CGRAEGLLELEFSTMLGKIPKPLNNLTLFPYYDNYIKLAHLEY
Sbjct: 61  STLAPHLQAIRESLILLCGRAEGLLELEFSTMLGKIPKPLNNLTLFPYYDNYIKLAHLEY 120

Query: 121 KILIDNGVVNPKKVAFVGSGPLPLTSIILAMQHMKNTYFDNYDIDSMANEVAGKIVASDG 180
           KILIDNGVVNPKKVAFVGSGPLPLTSIILAMQHMKNTYFDNYD+DSMANEVAGKIVASDG
Sbjct: 121 KILIDNGVVNPKKVAFVGSGPLPLTSIILAMQHMKNTYFDNYDVDSMANEVAGKIVASDG 180

Query: 181 ELEGRMKFWTRDIVEVGEELEGYDCVFLAALVGLKKEDKVKILSHLRKYMKQGGVLVVRS 240
           ELEGRMKFWTRDIVEVGEELEGYDCVFLAALVGL KEDKVKILSHLRKYMKQGGVLVVRS
Sbjct: 181 ELEGRMKFWTRDIVEVGEELEGYDCVFLAALVGLNKEDKVKILSHLRKYMKQGGVLVVRS 240

Query: 241 AKGGRAFLYPVVEVADMVGFDILSIFHPSDDVVNSVLLARKPILI 286
           A+GGRAFLYPVVEVADMVGFDILSIFHPSDDVVNSVLLARKPIL+
Sbjct: 241 ARGGRAFLYPVVEVADMVGFDILSIFHPSDDVVNSVLLARKPILL 285

[Close]

BLAST of CmaCh10G004030 vs. NCBI nr
Match: XP_023516742.1 (nicotianamine synthase-like [Cucurbita pepo subsp. pepo] >XP_023521836.1 nicotianamine synthase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 546.6 bits (1407), Expect = 1.3e-151
Identity = 273/285 (95.79%), Postives = 278/285 (97.54%), Query Frame = 0


Query: 1   MEALQILGTETDISEEVLIARVIDLHGSISRLETLKPCKEVNGLFTNLVKFCILPCSIDV 60
           M A QILGTETDISEE LIAR+IDLHGSISRLETLKPCKEVNGLFT LVKFCILPCSIDV
Sbjct: 1   MAAHQILGTETDISEEFLIARIIDLHGSISRLETLKPCKEVNGLFTKLVKFCILPCSIDV 60

Query: 61  STLPPHLQAIRESLILFCGRAEGLLELEFSTMLGKIPKPLNNLTLFPYYDNYIKLAHLEY 120
           +TLPPHLQAIRESLIL CGRAEGLLELEFSTMLGKIPKPLNNLTLFPYYDNYIKLAHLEY
Sbjct: 61  TTLPPHLQAIRESLILLCGRAEGLLELEFSTMLGKIPKPLNNLTLFPYYDNYIKLAHLEY 120

Query: 121 KILIDNGVVNPKKVAFVGSGPLPLTSIILAMQHMKNTYFDNYDIDSMANEVAGKIVASDG 180
           KILIDNG+VNPK+VAFVGSGPLPLTSIILAMQHMKNTYFDNYDIDSMANEVAGKIVASDG
Sbjct: 121 KILIDNGIVNPKRVAFVGSGPLPLTSIILAMQHMKNTYFDNYDIDSMANEVAGKIVASDG 180

Query: 181 ELEGRMKFWTRDIVEVGEELEGYDCVFLAALVGLKKEDKVKILSHLRKYMKQGGVLVVRS 240
           ELEGRMKFWTRDIVEVGEELEGYDCVFLAALVGLKKEDKVKILSHLRKYMKQGG LVVRS
Sbjct: 181 ELEGRMKFWTRDIVEVGEELEGYDCVFLAALVGLKKEDKVKILSHLRKYMKQGGALVVRS 240

Query: 241 AKGGRAFLYPVVEVADMVGFDILSIFHPSDDVVNSVLLARKPILI 286
           AKGGRAFLYPVV+VADM GFDILSIFHPSDDVVNSVLLARKPILI
Sbjct: 241 AKGGRAFLYPVVDVADMAGFDILSIFHPSDDVVNSVLLARKPILI 285

[Close]

BLAST of CmaCh10G004030 vs. NCBI nr
Match: XP_022922054.1 (nicotianamine synthase-like [Cucurbita moschata])

HSP 1 Score: 543.9 bits (1400), Expect = 8.3e-151
Identity = 274/285 (96.14%), Postives = 277/285 (97.19%), Query Frame = 0


Query: 1   MEALQILGTETDISEEVLIARVIDLHGSISRLETLKPCKEVNGLFTNLVKFCILPCSIDV 60
           M AL ILGTETDISEE LIARVIDLHGSISRLETLKPCKEVNGLFT+LVK CILPCSIDV
Sbjct: 1   MAALHILGTETDISEEFLIARVIDLHGSISRLETLKPCKEVNGLFTSLVKLCILPCSIDV 60

Query: 61  STLPPHLQAIRESLILFCGRAEGLLELEFSTMLGKIPKPLNNLTLFPYYDNYIKLAHLEY 120
           STL PHLQAIRESLIL CGRAEGLLELEFSTMLGKIPKPLNNLTLFPYY NYIKLAHLEY
Sbjct: 61  STLAPHLQAIRESLILLCGRAEGLLELEFSTMLGKIPKPLNNLTLFPYYGNYIKLAHLEY 120

Query: 121 KILIDNGVVNPKKVAFVGSGPLPLTSIILAMQHMKNTYFDNYDIDSMANEVAGKIVASDG 180
           KILIDNGVVNPKKVAFVGSGPLPLTSIILAMQHMKNTYFDNYD+DSMANEVAGKIVASDG
Sbjct: 121 KILIDNGVVNPKKVAFVGSGPLPLTSIILAMQHMKNTYFDNYDVDSMANEVAGKIVASDG 180

Query: 181 ELEGRMKFWTRDIVEVGEELEGYDCVFLAALVGLKKEDKVKILSHLRKYMKQGGVLVVRS 240
           ELEGRMKFWTRDIVEVGEELEGYDCVFLAALVGL KEDKVKILSHLRKYMKQGGVLVVRS
Sbjct: 181 ELEGRMKFWTRDIVEVGEELEGYDCVFLAALVGLNKEDKVKILSHLRKYMKQGGVLVVRS 240

Query: 241 AKGGRAFLYPVVEVADMVGFDILSIFHPSDDVVNSVLLARKPILI 286
           AKGGRAFLYPVVEVADMVGFDILSIFHPSDDVVNSVLLARKPIL+
Sbjct: 241 AKGGRAFLYPVVEVADMVGFDILSIFHPSDDVVNSVLLARKPILL 285

[Close]

BLAST of CmaCh10G004030 vs. TAIR 10
Match: AT1G56430.1 (nicotianamine synthase 4 )

HSP 1 Score: 281.2 bits (718), Expect = 9.4e-76
Identity = 139/271 (51.29%), Postives = 203/271 (74.91%), Query Frame = 0


Query: 15  EEVLIARVIDLHGSISRLETLKPCKEVNGLFTNLVKFCILP-CSIDVSTLPPHLQAIRES 74
           ++ L+ ++ DL+  IS+LETLKPC++V+ LF  LV  CI P  +IDV+ +  ++Q +R +
Sbjct: 6   DDQLVNKICDLYEKISKLETLKPCEDVDTLFKQLVSTCIPPNPNIDVTKMSENIQEMRSN 65

Query: 75  LILFCGRAEGLLELEFSTMLGKI-PKPLNNLTLFPYYDNYIKLAHLEYKILID--NGVVN 134
           LI  CG AEG LE  FS++L      PL++L LFPYY+NY+KL+ LE+ +L    NG V 
Sbjct: 66  LIKICGEAEGYLEHHFSSILTSFEDNPLHHLNLFPYYNNYLKLSKLEFDLLEQNLNGFV- 125

Query: 135 PKKVAFVGSGPLPLTSIILAMQHMKNTYFDNYDIDSMANEVAGKIVASDGELEGRMKFWT 194
           P+ VAF+GSGPLPLTS++LA  H+K++ F N+DID  AN VA ++V+SD +L  RM F T
Sbjct: 126 PRTVAFIGSGPLPLTSVVLASSHLKDSIFHNFDIDPSANMVAARLVSSDPDLSQRMFFHT 185

Query: 195 RDIVEVGEELEGYDCVFLAALVGLKKEDKVKILSHLRKYMKQGGVLVVRSAKGGRAFLYP 254
            DI++V E L+G+D VFLAALVG+ K++KVK++ HL K+M  G +L++RSA G RAFLYP
Sbjct: 186 VDIMDVTESLKGFDVVFLAALVGMDKKEKVKVVEHLEKHMSPGALLMLRSAHGPRAFLYP 245

Query: 255 VVEVADMVGFDILSIFHPSDDVVNSVLLARK 282
           +VE  D+ GF++LS++HP+D+V+NS++++RK
Sbjct: 246 IVEPCDLEGFEVLSVYHPTDEVINSIVISRK 275

[Close]

BLAST of CmaCh10G004030 vs. TAIR 10
Match: AT1G09240.1 (nicotianamine synthase 3 )

HSP 1 Score: 274.6 bits (701), Expect = 8.8e-74
Identity = 143/271 (52.77%), Postives = 198/271 (73.06%), Query Frame = 0


Query: 15  EEVLIARVIDLHGSISRLETLKPCKEVNGLFTNLVKFCILP-CSIDVSTLPPHLQAIRES 74
           +E L+  + DL+  IS+LE+LKP ++VN LF  LV  CI P  +IDV+ +   +Q IR +
Sbjct: 5   DEQLVQTICDLYEKISKLESLKPSEDVNILFKQLVSTCIPPNPNIDVTKMCDRVQEIRLN 64

Query: 75  LILFCGRAEGLLELEFSTMLGKI-PKPLNNLTLFPYYDNYIKLAHLEYKILID--NGVVN 134
           LI  CG AEG LE  FS++L      PL++L +FPYY+NY+KL  LE+ +L    NG V 
Sbjct: 65  LIKICGLAEGHLENHFSSILTSYQDNPLHHLNIFPYYNNYLKLGKLEFDLLEQNLNGFV- 124

Query: 135 PKKVAFVGSGPLPLTSIILAMQHMKNTYFDNYDIDSMANEVAGKIVASDGELEGRMKFWT 194
           PK VAF+GSGPLPLTSI+LA  H+K+T F N+DID  AN +A  +V+SD ++  RM F T
Sbjct: 125 PKSVAFIGSGPLPLTSIVLASFHLKDTIFHNFDIDPSANSLASLLVSSDPDISQRMFFHT 184

Query: 195 RDIVEVGEELEGYDCVFLAALVGLKKEDKVKILSHLRKYMKQGGVLVVRSAKGGRAFLYP 254
            DI++V E L+ +D VFLAALVG+ KE+KVK++ HL+K+M  G VL++RSA G RAFLYP
Sbjct: 185 VDIMDVTESLKSFDVVFLAALVGMNKEEKVKVIEHLQKHMAPGAVLMLRSAHGPRAFLYP 244

Query: 255 VVEVADMVGFDILSIFHPSDDVVNSVLLARK 282
           +VE  D+ GF++LSI+HP+DDV+NSV++++K
Sbjct: 245 IVEPCDLQGFEVLSIYHPTDDVINSVVISKK 274

[Close]

BLAST of CmaCh10G004030 vs. TAIR 10
Match: AT5G04950.1 (nicotianamine synthase 1 )

HSP 1 Score: 267.3 bits (682), Expect = 1.4e-71
Identity = 135/269 (50.19%), Postives = 190/269 (70.63%), Query Frame = 0


Query: 17  VLIARVIDLHGSISRLETLKPCKEVNGLFTNLVKFCI-LPCSIDVSTLPPHLQAIRESLI 76
           +++ ++IDL+  IS+L++LKP K V+ LF  LV  C+    +IDV+ +   ++ +R +LI
Sbjct: 7   LVVKQIIDLYDQISKLKSLKPSKNVDTLFGQLVSTCLPTDTNIDVTNMCEEVKDMRANLI 66

Query: 77  LFCGRAEGLLELEFSTMLGKIPK---PLNNLTLFPYYDNYIKLAHLEYKILIDNGVVNPK 136
             CG AEG LE  FST+LG + +   PL++L +FPYY NY+KL  LE+ +L  +    P 
Sbjct: 67  KLCGEAEGYLEQHFSTILGSLQEDQNPLDHLHIFPYYSNYLKLGKLEFDLLSQHSSHVPT 126

Query: 137 KVAFVGSGPLPLTSIILAMQHMKNTYFDNYDIDSMANEVAGKIVASDGELEGRMKFWTRD 196
           K+AFVGSGP+PLTSI+LA  H+ NT F N+DIDS AN +A  +V+ D +L  RM F T D
Sbjct: 127 KIAFVGSGPMPLTSIVLAKFHLPNTTFHNFDIDSHANTLASNLVSRDPDLSKRMIFHTTD 186

Query: 197 IVEVGEELEGYDCVFLAALVGLKKEDKVKILSHLRKYMKQGGVLVVRSAKGGRAFLYPVV 256
           ++   E L+ YD VFLAALVG+ KE KVK + HL K+M  G VL++RSA   RAFLYP+V
Sbjct: 187 VLNATEGLDQYDVVFLAALVGMDKESKVKAIEHLEKHMAPGAVLMLRSAHALRAFLYPIV 246

Query: 257 EVADMVGFDILSIFHPSDDVVNSVLLARK 282
           + +D+ GF +L+I+HP+DDVVNSV++ARK
Sbjct: 247 DSSDLKGFQLLTIYHPTDDVVNSVVIARK 275

[Close]

BLAST of CmaCh10G004030 vs. TAIR 10
Match: AT5G56080.1 (nicotianamine synthase 2 )

HSP 1 Score: 266.9 bits (681), Expect = 1.8e-71
Identity = 136/269 (50.56%), Postives = 185/269 (68.77%), Query Frame = 0


Query: 17  VLIARVIDLHGSISRLETLKPCKEVNGLFTNLVKFCILPCSIDVSTLPPH---LQAIRES 76
           +++ +++DL+  IS LE+LKP K V+ LF  LV  C LP   ++     H   ++ +R  
Sbjct: 7   LVVKQIMDLYNQISNLESLKPSKNVDTLFRQLVSTC-LPTDTNIDVTEIHDEKVKDMRSH 66

Query: 77  LILFCGRAEGLLELEFSTMLGKI-PKPLNNLTLFPYYDNYIKLAHLEYKILIDNGVVNPK 136
           LI  CG AEG LE  FS +LG     PLN+L +FPYY+NY+KL  LE+ +L  +    P 
Sbjct: 67  LIKLCGEAEGYLEQHFSAILGSFEDNPLNHLHIFPYYNNYLKLGKLEFDLLSQHTTHVPT 126

Query: 137 KVAFVGSGPLPLTSIILAMQHMKNTYFDNYDIDSMANEVAGKIVASDGELEGRMKFWTRD 196
           KVAF+GSGP+PLTSI+LA  H+ NT F N+DIDS AN +A  +V+ D +L  RM F T D
Sbjct: 127 KVAFIGSGPMPLTSIVLAKFHLPNTTFHNFDIDSHANTLASNLVSRDSDLSKRMIFHTTD 186

Query: 197 IVEVGEELEGYDCVFLAALVGLKKEDKVKILSHLRKYMKQGGVLVVRSAKGGRAFLYPVV 256
           ++   E L+ YD VFLAALVG+ KE KVK + HL K+M  G V+++RSA G RAFLYP+V
Sbjct: 187 VLNAKEGLDQYDVVFLAALVGMDKESKVKAIEHLEKHMAPGAVVMLRSAHGLRAFLYPIV 246

Query: 257 EVADMVGFDILSIFHPSDDVVNSVLLARK 282
           +  D+ GF++L+I+HPSDDVVNSV++ARK
Sbjct: 247 DSCDLKGFEVLTIYHPSDDVVNSVVIARK 274

[Close]

BLAST of CmaCh10G004030 vs. TAIR 10
Match: AT4G26483.1 (nicotianamine synthases )

HSP 1 Score: 56.2 bits (134), Expect = 4.9e-08
Identity = 28/54 (51.85%), Postives = 40/54 (74.07%), Query Frame = 0


Query: 230 MKQGGVLVVRSAKGGRAFLYPVVEVADMVGFDILSIFHP--SDDVVNSVLLARK 282
           M  G +L++RSA+G R+FLY  V+  D+ GF++L I+HP  SD  VNSV++ARK
Sbjct: 1   MDPGALLMLRSARGLRSFLYVDVDPCDLKGFEVLEIYHPSMSDGFVNSVMVARK 54

The following BLAST results are available for this feature:

BLAST of CmaCh10G004030 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9XGI7	1.2e-78	54.92	Nicotianamine synthase OS=Solanum lycopersicum OX=4081 GN=CHLN PE=2 SV=1	[more]
Q9C7X5	1.3e-74	51.29	Probable nicotianamine synthase 4 OS=Arabidopsis thaliana OX=3702 GN=NAS4 PE=2 S...	[more]
O80483	1.2e-72	52.77	Nicotianamine synthase 3 OS=Arabidopsis thaliana OX=3702 GN=NAS3 PE=2 SV=1	[more]
Q9FF79	2.0e-70	50.19	Nicotianamine synthase 1 OS=Arabidopsis thaliana OX=3702 GN=NAS1 PE=2 SV=1	[more]
Q9FKT9	2.6e-70	50.56	Nicotianamine synthase 2 OS=Arabidopsis thaliana OX=3702 GN=NAS2 PE=2 SV=1	[more]

BLAST of CmaCh10G004030 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1JBQ8	1.3e-157	100.00	nicotianamine synthase-like OS=Cucurbita maxima OX=3661 GN=LOC111485338 PE=3 SV=...	[more]
A0A6J1E292	4.0e-151	96.14	nicotianamine synthase-like OS=Cucurbita moschata OX=3662 GN=LOC111430119 PE=3 S...	[more]
A0A0A0LUJ7	9.3e-124	79.00	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G423010 PE=3 SV=1	[more]
A0A5D3CIT2	1.2e-123	79.00	Nicotianamine synthase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaf...	[more]
A0A1S3BXH0	1.2e-123	79.00	nicotianamine synthase-like OS=Cucumis melo OX=3656 GN=LOC103494275 PE=3 SV=1	[more]

BLAST of CmaCh10G004030 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022987937.1	2.7e-157	100.00	nicotianamine synthase-like [Cucurbita maxima]	[more]
KAG6589715.1	2.0e-152	96.84	Nicotianamine synthase, partial [Cucurbita argyrosperma subsp. sororia]	[more]
KAG7023395.1	7.5e-152	96.49	Nicotianamine synthase, partial [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_023516742.1	1.3e-151	95.79	nicotianamine synthase-like [Cucurbita pepo subsp. pepo] >XP_023521836.1 nicotia...	[more]
XP_022922054.1	8.3e-151	96.14	nicotianamine synthase-like [Cucurbita moschata]	[more]

BLAST of CmaCh10G004030 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G56430.1	9.4e-76	51.29	nicotianamine synthase 4	[more]
AT1G09240.1	8.8e-74	52.77	nicotianamine synthase 3	[more]
AT5G04950.1	1.4e-71	50.19	nicotianamine synthase 1	[more]
AT5G56080.1	1.8e-71	50.56	nicotianamine synthase 2	[more]
AT4G26483.1	4.9e-08	51.85	nicotianamine synthases	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR004298	Nicotianamine synthase	PFAM	PF03059	NAS	coord: 15..282 e-value: 4.9E-104 score: 347.5
IPR004298	Nicotianamine synthase	PANTHER	PTHR32266	NICOTIANAMINE SYNTHASE 3	coord: 2..283
IPR004298	Nicotianamine synthase	PROSITE	PS51142	NAS	coord: 11..285 score: 48.330662
None	No IPR available	GENE3D	3.40.50.150	Vaccinia Virus protein VP39	coord: 24..282 e-value: 1.2E-58 score: 200.4
None	No IPR available	PANTHER	PTHR32266:SF11	NICOTIANAMINE SYNTHASE	coord: 2..283
IPR029063	S-adenosyl-L-methionine-dependent methyltransferase	SUPERFAMILY	53335	S-adenosyl-L-methionine-dependent methyltransferases	coord: 125..240

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmaCh10G004030.1	CmaCh10G004030.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0030418	nicotianamine biosynthetic process
molecular_function	GO:0030410	nicotianamine synthase activity