CmaCh10G001780 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh10G001780
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Descriptionprotein NRT1/ PTR FAMILY 2.10-like
LocationCma_Chr10: 815271 .. 817276 (-)
RNA-Seq ExpressionCmaCh10G001780
SyntenyCmaCh10G001780
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAGAGAAATAATGAAGATGTTCATAATCTTGATGAGGCTAAGGTCAATTATAAAGGAGTCAAGGCCTTGCCATTTGTAGTAGGTTCGTAACATACCTCAATTTGTTTTGTTTTGATACATGTAATGCTAATTAGTACGTGGGTTTGTTGATTTAGGGAATGAAACTTTTGAGAAGCTGGGGACTACGGGCACTTCTTCAAACCTTTTGGTGTATTTGACAGATGTATTTCATATGAAAACCATCACTGCCACCACTCTTATGAACATTTTCCACGGCAGCACCAACTTTTCCACTTTGTTTGGAGCATTTCTTTGTGACACTTATTTTGGACGTTACAAGACCTTAGGATATGCCTCCATTGCTTCATTAATGGTACGCCTGCTGCTATTGTTTCACTTTCTCTTATAAAAACATATATAAGGATTGAATAATTTGGTTGCTTTCATGTCCCTGATCACTCGCACAGGGGATGGTTGTACTTACACTGACAGCAACAATTGAGAGGCTGCACCCACCAGACTGTGGAAAGGGCAGTGCCGCAGGCGGGGCTTGCCTAGAGCCAACGCCATGGCAGATAGCATTTCTGTTATTTGGATTGGGACTGCTCATAATCGGAGCTGGGGGTATCAGACCCTGCAACTTGGCGTTTGGAGCCGACCAATTCAATCCCAACACAGCCTCCGGGAAGTTGGGAATAAACAGCTTCTTCAACTGGTACTACTTCACCTTCACATTTGCCATGATCATATCTCTCACCATCATTGTGTATGTCCAAACTGAAGTCAGCTGGGCTTGGGGATTGGCAATCCCTGCGTTCCTCATGTTCCTGTCCTGTGCACTTTTCTTCATGGGTTCTTGGATTTACGTCAAATTGGAACCCCAAGGGAGTCCTTTCACCAGTGTGATGCGAGTCCTAGTGGCTGCTCTCAAGAAAAGGCGCCTGCCTTCGCCGGACCAGCAATGGCCATCTCTTTTTAACCACATTCCCTCAAACTCCATCAATTCCAAGCTTCCTTACACTGATCAATTCAGGTGGGTCTCCACTTTCAAAGTTTGAGTCTTTGGTTAAAAGAGAATAACCCCTTTTATTGCTTTCAGCTTCCTAAACAAGGCGGCAATCATAACCCCAGAAGACAAATTCAAGTCAGATGGCTCTGCAGACGATCCATGGACACTCTGCAGCACACAACAAGTGGAAGAAGTGAAATGCTTGGTGAGAGTGATACCAATATGGGGTGCAGCCATAATGTACCATGTCGCCACCACGCAGCAACAAACATACGTCGTCTTCCAAGCCCTCCAATCCGACAGGCGCTTGTTCTTCGGGAACACTCATTATTTCAAAATCCCAGCCGCCTCCTACACCATATTCACAATGATTGGCCTCACCATCTGGATCCCTTTCTACGACAGAATCGTAGTCCCTTCTCTGCGCCGAATCACCTCTAGAGAAGGCGGCATCACGCTCCTTCAAAAAATGGGGATTGGAATGGTCATTGCCATTGTAACCATGTTCATCTCAGCATTAGTGGAACAAAAGAGGAGGAATCTGGCCCTCACACAGCCCCTGTGCGAAGAAACAGGGAGAAGGGCTGCAATTTCTTCCATGTCTGCGCTCTGGTTGGTCCCTCAACTGACGCTGATTGGGTTGTCCGAGGCGTTCACCGTTATTGCCCAAGTCGAATTTTACTACAAGGAGTTCCCTGAGAACATGAGGAGCATTGGGGGGTCGTTGTCTTTTGTGGGTCTTGCGCTGTCGAACTATTTGAGTGGGTTCATGGTGACGGTGGTGCACAGCCTCACCACCGGCAACTGGCTGCCGGAAGATCTGAACGAGGGGCGATTGGATTATTTCTATTTCATGGTGTCGGGATTGGAAGCCGTGAACTTGGGGTACTTCGTGTTGTGTTCTAAGTGGTACAAGTACAAGGGGAGTGGAAGCCATGGCGTTGATGAGATGGACTTTGGGAAGACAGAATTTGAGAAAACTGTGGTTTATTGA

mRNA sequence

ATGGAGAGAAATAATGAAGATGTTCATAATCTTGATGAGGCTAAGGTCAATTATAAAGGAGTCAAGGCCTTGCCATTTGTAGTAGGGAATGAAACTTTTGAGAAGCTGGGGACTACGGGCACTTCTTCAAACCTTTTGGTGTATTTGACAGATGTATTTCATATGAAAACCATCACTGCCACCACTCTTATGAACATTTTCCACGGCAGCACCAACTTTTCCACTTTGTTTGGAGCATTTCTTTGTGACACTTATTTTGGACGTTACAAGACCTTAGGATATGCCTCCATTGCTTCATTAATGGGGATGGTTGTACTTACACTGACAGCAACAATTGAGAGGCTGCACCCACCAGACTGTGGAAAGGGCAGTGCCGCAGGCGGGGCTTGCCTAGAGCCAACGCCATGGCAGATAGCATTTCTGTTATTTGGATTGGGACTGCTCATAATCGGAGCTGGGGGTATCAGACCCTGCAACTTGGCGTTTGGAGCCGACCAATTCAATCCCAACACAGCCTCCGGGAAGTTGGGAATAAACAGCTTCTTCAACTGGTACTACTTCACCTTCACATTTGCCATGATCATATCTCTCACCATCATTGTGTATGTCCAAACTGAAGTCAGCTGGGCTTGGGGATTGGCAATCCCTGCGTTCCTCATGTTCCTGTCCTGTGCACTTTTCTTCATGGGTTCTTGGATTTACGTCAAATTGGAACCCCAAGGGAGTCCTTTCACCAGTGTGATGCGAGTCCTAGTGGCTGCTCTCAAGAAAAGGCGCCTGCCTTCGCCGGACCAGCAATGGCCATCTCTTTTTAACCACATTCCCTCAAACTCCATCAATTCCAAGCTTCCTTACACTGATCAATTCAGCTTCCTAAACAAGGCGGCAATCATAACCCCAGAAGACAAATTCAAGTCAGATGGCTCTGCAGACGATCCATGGACACTCTGCAGCACACAACAAGTGGAAGAAGTGAAATGCTTGGTGAGAGTGATACCAATATGGGGTGCAGCCATAATGTACCATGTCGCCACCACGCAGCAACAAACATACGTCGTCTTCCAAGCCCTCCAATCCGACAGGCGCTTGTTCTTCGGGAACACTCATTATTTCAAAATCCCAGCCGCCTCCTACACCATATTCACAATGATTGGCCTCACCATCTGGATCCCTTTCTACGACAGAATCGTAGTCCCTTCTCTGCGCCGAATCACCTCTAGAGAAGGCGGCATCACGCTCCTTCAAAAAATGGGGATTGGAATGGTCATTGCCATTGTAACCATGTTCATCTCAGCATTAGTGGAACAAAAGAGGAGGAATCTGGCCCTCACACAGCCCCTGTGCGAAGAAACAGGGAGAAGGGCTGCAATTTCTTCCATGTCTGCGCTCTGGTTGGTCCCTCAACTGACGCTGATTGGGTTGTCCGAGGCGTTCACCGTTATTGCCCAAGTCGAATTTTACTACAAGGAGTTCCCTGAGAACATGAGGAGCATTGGGGGGTCGTTGTCTTTTGTGGGTCTTGCGCTGTCGAACTATTTGAGTGGGTTCATGGTGACGGTGGTGCACAGCCTCACCACCGGCAACTGGCTGCCGGAAGATCTGAACGAGGGGCGATTGGATTATTTCTATTTCATGGTGTCGGGATTGGAAGCCGTGAACTTGGGGTACTTCGTGTTGTGTTCTAAGTGGTACAAGTACAAGGGGAGTGGAAGCCATGGCGTTGATGAGATGGACTTTGGGAAGACAGAATTTGAGAAAACTGTGGTTTATTGA

Coding sequence (CDS)

ATGGAGAGAAATAATGAAGATGTTCATAATCTTGATGAGGCTAAGGTCAATTATAAAGGAGTCAAGGCCTTGCCATTTGTAGTAGGGAATGAAACTTTTGAGAAGCTGGGGACTACGGGCACTTCTTCAAACCTTTTGGTGTATTTGACAGATGTATTTCATATGAAAACCATCACTGCCACCACTCTTATGAACATTTTCCACGGCAGCACCAACTTTTCCACTTTGTTTGGAGCATTTCTTTGTGACACTTATTTTGGACGTTACAAGACCTTAGGATATGCCTCCATTGCTTCATTAATGGGGATGGTTGTACTTACACTGACAGCAACAATTGAGAGGCTGCACCCACCAGACTGTGGAAAGGGCAGTGCCGCAGGCGGGGCTTGCCTAGAGCCAACGCCATGGCAGATAGCATTTCTGTTATTTGGATTGGGACTGCTCATAATCGGAGCTGGGGGTATCAGACCCTGCAACTTGGCGTTTGGAGCCGACCAATTCAATCCCAACACAGCCTCCGGGAAGTTGGGAATAAACAGCTTCTTCAACTGGTACTACTTCACCTTCACATTTGCCATGATCATATCTCTCACCATCATTGTGTATGTCCAAACTGAAGTCAGCTGGGCTTGGGGATTGGCAATCCCTGCGTTCCTCATGTTCCTGTCCTGTGCACTTTTCTTCATGGGTTCTTGGATTTACGTCAAATTGGAACCCCAAGGGAGTCCTTTCACCAGTGTGATGCGAGTCCTAGTGGCTGCTCTCAAGAAAAGGCGCCTGCCTTCGCCGGACCAGCAATGGCCATCTCTTTTTAACCACATTCCCTCAAACTCCATCAATTCCAAGCTTCCTTACACTGATCAATTCAGCTTCCTAAACAAGGCGGCAATCATAACCCCAGAAGACAAATTCAAGTCAGATGGCTCTGCAGACGATCCATGGACACTCTGCAGCACACAACAAGTGGAAGAAGTGAAATGCTTGGTGAGAGTGATACCAATATGGGGTGCAGCCATAATGTACCATGTCGCCACCACGCAGCAACAAACATACGTCGTCTTCCAAGCCCTCCAATCCGACAGGCGCTTGTTCTTCGGGAACACTCATTATTTCAAAATCCCAGCCGCCTCCTACACCATATTCACAATGATTGGCCTCACCATCTGGATCCCTTTCTACGACAGAATCGTAGTCCCTTCTCTGCGCCGAATCACCTCTAGAGAAGGCGGCATCACGCTCCTTCAAAAAATGGGGATTGGAATGGTCATTGCCATTGTAACCATGTTCATCTCAGCATTAGTGGAACAAAAGAGGAGGAATCTGGCCCTCACACAGCCCCTGTGCGAAGAAACAGGGAGAAGGGCTGCAATTTCTTCCATGTCTGCGCTCTGGTTGGTCCCTCAACTGACGCTGATTGGGTTGTCCGAGGCGTTCACCGTTATTGCCCAAGTCGAATTTTACTACAAGGAGTTCCCTGAGAACATGAGGAGCATTGGGGGGTCGTTGTCTTTTGTGGGTCTTGCGCTGTCGAACTATTTGAGTGGGTTCATGGTGACGGTGGTGCACAGCCTCACCACCGGCAACTGGCTGCCGGAAGATCTGAACGAGGGGCGATTGGATTATTTCTATTTCATGGTGTCGGGATTGGAAGCCGTGAACTTGGGGTACTTCGTGTTGTGTTCTAAGTGGTACAAGTACAAGGGGAGTGGAAGCCATGGCGTTGATGAGATGGACTTTGGGAAGACAGAATTTGAGAAAACTGTGGTTTATTGA

Protein sequence

MERNNEDVHNLDEAKVNYKGVKALPFVVGNETFEKLGTTGTSSNLLVYLTDVFHMKTITATTLMNIFHGSTNFSTLFGAFLCDTYFGRYKTLGYASIASLMGMVVLTLTATIERLHPPDCGKGSAAGGACLEPTPWQIAFLLFGLGLLIIGAGGIRPCNLAFGADQFNPNTASGKLGINSFFNWYYFTFTFAMIISLTIIVYVQTEVSWAWGLAIPAFLMFLSCALFFMGSWIYVKLEPQGSPFTSVMRVLVAALKKRRLPSPDQQWPSLFNHIPSNSINSKLPYTDQFSFLNKAAIITPEDKFKSDGSADDPWTLCSTQQVEEVKCLVRVIPIWGAAIMYHVATTQQQTYVVFQALQSDRRLFFGNTHYFKIPAASYTIFTMIGLTIWIPFYDRIVVPSLRRITSREGGITLLQKMGIGMVIAIVTMFISALVEQKRRNLALTQPLCEETGRRAAISSMSALWLVPQLTLIGLSEAFTVIAQVEFYYKEFPENMRSIGGSLSFVGLALSNYLSGFMVTVVHSLTTGNWLPEDLNEGRLDYFYFMVSGLEAVNLGYFVLCSKWYKYKGSGSHGVDEMDFGKTEFEKTVVY
Homology
BLAST of CmaCh10G001780 vs. ExPASy Swiss-Prot
Match: Q944G5 (Protein NRT1/ PTR FAMILY 2.10 OS=Arabidopsis thaliana OX=3702 GN=NPF2.10 PE=1 SV=3)

HSP 1 Score: 682.2 bits (1759), Expect = 5.3e-195
Identity = 332/576 (57.64%), Postives = 425/576 (73.78%), Query Frame = 0

Query: 12  DEAKVNYKGVKALPFVVGNETFEKLGTTGTSSNLLVYLTDVFHMKTITATTLMNIFHGST 71
           ++ K+ Y+G K +PF++GNETFEKLG  GT SNLLVYLT VF++K+ TA T++N F G+ 
Sbjct: 56  EQRKIVYRGWKVMPFIIGNETFEKLGIIGTLSNLLVYLTSVFNLKSYTAATIINAFSGTI 115

Query: 72  NFSTLFGAFLCDTYFGRYKTLGYASIASLMGMVVLTLTATIERLHPPDCGKGSAAGGACL 131
           NF T   AFLCDTYFGRYKTL  A IA  +G  V+ LTA I  LHP  CG       +C 
Sbjct: 116 NFGTFIAAFLCDTYFGRYKTLSVAVIACFLGSFVILLTAAIPSLHPVACGNKI----SCE 175

Query: 132 EPTPWQIAFLLFGLGLLIIGAGGIRPCNLAFGADQFNPNTASGKLGINSFFNWYYFTFTF 191
            P+  QI FLL GLG L++GAGGIRPCNLAFGADQFNP + SGK GINSFFNWY+FTFTF
Sbjct: 176 GPSVGQILFLLMGLGFLVVGAGGIRPCNLAFGADQFNPKSESGKKGINSFFNWYFFTFTF 235

Query: 192 AMIISLTIIVYVQTEVSWAWGLAIPAFLMFLSCALFFMGSWIYVKLEPQGSPFTSVMRVL 251
           A IISLT +VY+Q+ VSW  GL IP  LMFL+C +FF G  +YVK++  GSP   + RV+
Sbjct: 236 AQIISLTAVVYIQSNVSWTIGLIIPVALMFLACVIFFAGDRLYVKVKASGSPLAGIARVI 295

Query: 252 VAALKKRRLPSPDQQWPSLFNHIPSNSINSKLPYTDQFSFLNKAAIITPEDKFKSDGSAD 311
            AA+KKR L    Q W +L+NHIPSN  N+ L YTDQF FL+KAAI+TPE+K  SDG+A 
Sbjct: 296 AAAIKKRGLKPVKQPWVNLYNHIPSNYANTTLKYTDQFRFLDKAAIMTPEEKLNSDGTAS 355

Query: 312 DPWTLCSTQQVEEVKCLVRVIPIWGAAIMYHVATTQQQTYVVFQALQSDRRLFFGNTHYF 371
           DPW LC+ QQVEEVKC+VRVIPIW A+ +Y++A T Q TY VFQALQSDRRL  G    F
Sbjct: 356 DPWKLCTLQQVEEVKCIVRVIPIWFASTIYYLAITIQMTYPVFQALQSDRRLGSGG---F 415

Query: 372 KIPAASYTIFTMIGLTIWIPFYDRIVVPSLRRITSREGGITLLQKMGIGMVIAIVTMFIS 431
           +IPAA+Y +F M G+T++I FYDR++VPSLRR+T  E GI+LLQ++G G   AI+++ +S
Sbjct: 416 RIPAATYVVFLMTGMTVFIIFYDRVLVPSLRRVTGLETGISLLQRIGAGFTFAIMSLLVS 475

Query: 432 ALVEQKRRNLALTQPLCEETGRRAAISSMSALWLVPQLTLIGLSEAFTVIAQVEFYYKEF 491
             +E++RRN ALT+P      R   ISSMSALWL+PQLTL G++EAF  I Q+EFYYK+F
Sbjct: 476 GFIEERRRNFALTKPTLGMAPRTGEISSMSALWLIPQLTLAGIAEAFAAIGQMEFYYKQF 535

Query: 492 PENMRSIGGSLSFVGLALSNYLSGFMVTVVHSLT----TGNWLPEDLNEGRLDYFYFMVS 551
           PENM+S  GS+ +VG  +S+YL+ F+++ VH  T    +GNWL EDLN+ +LDYFYFM++
Sbjct: 536 PENMKSFAGSIFYVGAGVSSYLASFLISTVHRTTAHSPSGNWLAEDLNKAKLDYFYFMLT 595

Query: 552 GLEAVNLGYFVLCSKWYKYKGSGSHGVDEMDFGKTE 584
           GL  VN+ YF+L ++WY+YKG     + E++  + E
Sbjct: 596 GLMVVNMAYFLLMARWYRYKGGNDEDITEIETNEEE 624

BLAST of CmaCh10G001780 vs. ExPASy Swiss-Prot
Match: Q9LV10 (Protein NRT1/ PTR FAMILY 2.11 OS=Arabidopsis thaliana OX=3702 GN=NPF2.11 PE=1 SV=1)

HSP 1 Score: 666.4 bits (1718), Expect = 3.0e-190
Identity = 329/568 (57.92%), Postives = 419/568 (73.77%), Query Frame = 0

Query: 6   EDVHNLDEAKVNYKGVKALPFVVGNETFEKLGTTGTSSNLLVYLTDVFHMKTITATTLMN 65
           EDV N  + KV Y+G K +PF++GNETFEKLG  GT SNLLVYLT VF++K+ITA T++N
Sbjct: 34  EDVQN--QKKVVYRGWKVMPFIIGNETFEKLGIIGTLSNLLVYLTAVFNLKSITAATIIN 93

Query: 66  IFHGSTNFSTLFGAFLCDTYFGRYKTLGYASIASLMGMVVLTLTATIERLHPPDCGKGSA 125
            F G+ NF T   AFLCDTYFGRYKTL  A IA  +G  V+ LTA + +LHP  C  G+A
Sbjct: 94  AFSGTINFGTFVAAFLCDTYFGRYKTLSVAVIACFLGSFVILLTAAVPQLHPAAC--GTA 153

Query: 126 AGGACLEPTPWQIAFLLFGLGLLIIGAGGIRPCNLAFGADQFNPNTASGKLGINSFFNWY 185
           A   C  P+  QIAFLL GLG L++GAGGIRPCNLAFGADQFNP + SGK GI+SFFNWY
Sbjct: 154 ADSICNGPSGGQIAFLLMGLGFLVVGAGGIRPCNLAFGADQFNPKSESGKRGIDSFFNWY 213

Query: 186 YFTFTFAMIISLTIIVYVQTEVSWAWGLAIPAFLMFLSCALFFMGSWIYVKLEPQGSPFT 245
           +FTFTFA I+SLT++VYVQ+ VSW  GL IPA LMFL+C +FF G  +YVK++  GSP  
Sbjct: 214 FFTFTFAQILSLTLVVYVQSNVSWTIGLTIPAVLMFLACLIFFAGDKLYVKIKASGSPLA 273

Query: 246 SVMRVLVAALKKRRLPSPDQQWPSLFNHIPSNSINSKLPYTDQFSFLNKAAIITPEDKFK 305
            + +V+  A+KKR L    Q W +L+N+ P    NSKL YTDQF FL+KAAI+TPEDK +
Sbjct: 274 GIAQVIAVAIKKRGLKPAKQPWLNLYNYYPPKYANSKLKYTDQFRFLDKAAILTPEDKLQ 333

Query: 306 SDGSADDPWTLCSTQQVEEVKCLVRVIPIWGAAIMYHVATTQQQTYVVFQALQSDRRLFF 365
            DG   DPW LC+ QQVEEVKC+VRV+PIW A+ +Y++  TQQ TY VFQALQSDRRL  
Sbjct: 334 PDGKPADPWKLCTMQQVEEVKCIVRVLPIWFASSIYYLTITQQMTYPVFQALQSDRRLGS 393

Query: 366 GNTHYFKIPAASYTIFTMIGLTIWIPFYDRIVVPSLRRITSREGGITLLQKMGIGMVIAI 425
           G    F IPAA+Y +F M G+T++I  YDR++VP++RRIT  + GITLLQ++G G+  A 
Sbjct: 394 GG---FVIPAATYVVFLMTGMTVFIVVYDRVLVPTMRRITGLDTGITLLQRIGTGIFFAT 453

Query: 426 VTMFISALVEQKRRNLALTQPLCEETGRRAAISSMSALWLVPQLTLIGLSEAFTVIAQVE 485
            ++ ++  VE++RR  ALT+P      R+  ISSMSA+WL+PQL+L G++EAF  I Q+E
Sbjct: 454 ASLVVAGFVEERRRTFALTKPTLGMAPRKGEISSMSAMWLIPQLSLAGVAEAFAAIGQME 513

Query: 486 FYYKEFPENMRSIGGSLSFVGLALSNYLSGFMVTVVHSLTT----GNWLPEDLNEGRLDY 545
           FYYK+FPENMRS  GS+ +VG  +S+YL  F++  VH  T     GNWL EDLN+GRLD 
Sbjct: 514 FYYKQFPENMRSFAGSIFYVGGGVSSYLGSFLIATVHRTTQNSSGGNWLAEDLNKGRLDL 573

Query: 546 FYFMVSGLEAVNLGYFVLCSKWYKYKGS 570
           FYFM++G+ AVN  YF++ S+WY+YKGS
Sbjct: 574 FYFMIAGILAVNFAYFLVMSRWYRYKGS 594

BLAST of CmaCh10G001780 vs. ExPASy Swiss-Prot
Match: Q9M9V7 (Protein NRT1/ PTR FAMILY 2.9 OS=Arabidopsis thaliana OX=3702 GN=NPF2.9 PE=1 SV=1)

HSP 1 Score: 649.0 bits (1673), Expect = 5.0e-185
Identity = 324/593 (54.64%), Postives = 433/593 (73.02%), Query Frame = 0

Query: 1   MERNNEDVHNLDEAKVNYKGVKALPFVVGNETFEKLGTTGTSSNLLVYLTDVFHMKTITA 60
           +E+  +++   DE+K+ Y+G K +PF++GNETFEKLG  G+SSNL++YLT VF+MK+ITA
Sbjct: 3   VEKTEKNIAEDDESKIIYRGWKVMPFIIGNETFEKLGIVGSSSNLVIYLTTVFNMKSITA 62

Query: 61  TTLMNIFHGSTNFSTLFGAFLCDTYFGRYKTLGYASIASLMGMVVLTLTATIERLHPPDC 120
             ++NI+ G++NF T+  AFLCD+YFGRYKTL +A IA  +G V + LTA I  LHP  C
Sbjct: 63  AKVVNIYGGTSNFGTIVAAFLCDSYFGRYKTLSFAMIACFLGSVAMDLTAVIHPLHPAQC 122

Query: 121 GKGSAAGGACLEPTPWQIAFLLFGLGLLIIGAGGIRPCNLAFGADQFNPNTASGKLGINS 180
            K    G  C  P+  QI FL   + LL+IGAGGIRPCNL FGADQF+P T  GK GI S
Sbjct: 123 AK--EIGSVCNGPSIGQIMFLAGAMVLLVIGAGGIRPCNLPFGADQFDPKTKEGKRGIES 182

Query: 181 FFNWYYFTFTFAMIISLTIIVYVQTEVSWAWGLAIPAFLMFLSCALFFMGSWIYVKLEPQ 240
           FFNWY+FTFTFA ++SLT+IVYVQ+ VSW+ GLAIPA LM L C +FF GS +YVK++  
Sbjct: 183 FFNWYFFTFTFAQMVSLTLIVYVQSNVSWSIGLAIPAILMLLGCIIFFAGSKLYVKVKAS 242

Query: 241 GSPFTSVMRVLVAALKKRRLPSPDQQWPSLFNHIPSNSINSKLPYTDQFSFLNKAAIITP 300
           GSP  S+ RV+V A+KKRRL         L+N+I S+  NSKL +T+QF FL+K+AI T 
Sbjct: 243 GSPIHSITRVIVVAIKKRRLKPVGPN--ELYNYIASDFKNSKLGHTEQFRFLDKSAIQTQ 302

Query: 301 EDKFKSDGSADDPWTLCSTQQVEEVKCLVRVIPIWGAAIMYHVATTQQQTYVVFQALQSD 360
           +DK   DGS  D W LCS QQVEEVKC++RV+P+W +A ++++A  QQ TY +FQ+LQSD
Sbjct: 303 DDKLNKDGSPVDAWKLCSMQQVEEVKCVIRVLPVWLSAALFYLAYIQQTTYTIFQSLQSD 362

Query: 361 RRLFFGNTHYFKIPAASYTIFTMIGLTIWIPFYDRIVVPSLRRITSREGGITLLQKMGIG 420
           RRL  G+   F+IPA SYT+F M+G+TI+IP YDR++VP LR+ T R+GGIT LQ++G G
Sbjct: 363 RRLGPGS---FQIPAGSYTVFLMLGMTIFIPIYDRVLVPFLRKYTGRDGGITQLQRVGAG 422

Query: 421 MVIAIVTMFISALVEQKRRNLALTQPLCEETGRRAAISSMSALWLVPQLTLIGLSEAFTV 480
           + + I +M +SA+VEQ RR +ALT+P      R+ AISSMS +WL+PQL L+G+++A   
Sbjct: 423 LFLCITSMMVSAIVEQYRRKVALTKPTLGLAPRKGAISSMSGMWLIPQLVLMGIADALAG 482

Query: 481 IAQVEFYYKEFPENMRSIGGSLSFVGLALSNYLSGFMVTVVHSLTT----GNWLPEDLNE 540
           + Q+EFYYK+FPENMRS  GSL + G+ L++YLS F+++ VH  T     G+WLPEDLN+
Sbjct: 483 VGQMEFYYKQFPENMRSFAGSLYYCGIGLASYLSTFLLSAVHDTTEGFSGGSWLPEDLNK 542

Query: 541 GRLDYFYFMVSGLEAVNLGYFVLCSKWYKYKGSGSHGVDEMDFGKTEFEKTVV 590
           GRL+YFYF+V+G+  +NL YF+L S WY+YK   +   D MD    EF+K  V
Sbjct: 543 GRLEYFYFLVAGMMTLNLAYFLLVSHWYRYKDVVAKDKD-MDKTSAEFDKVSV 587

BLAST of CmaCh10G001780 vs. ExPASy Swiss-Prot
Match: Q8RX77 (Protein NRT1/ PTR FAMILY 2.13 OS=Arabidopsis thaliana OX=3702 GN=NPF2.13 PE=1 SV=1)

HSP 1 Score: 484.6 bits (1246), Expect = 1.6e-135
Identity = 255/565 (45.13%), Postives = 370/565 (65.49%), Query Frame = 0

Query: 11  LDEAKVNYK--GVKALPFVVGNETFEKLGTTGTSSNLLVYLTDVFHMKTITATTLMNIFH 70
           LD  KV  K  G +A+ F++GNET E+LG+ G  +N +VYLT VFH++ + A  ++NI+ 
Sbjct: 43  LDAEKVEKKPGGWRAVSFILGNETLERLGSIGLLANFMVYLTKVFHLEQVDAANVINIWS 102

Query: 71  GSTNFSTLFGAFLCDTYFGRYKTLGYASIASLMGMVVLTLTATIERLHPPDCGKGSAAGG 130
           G TN + L GA++ DTY GR+KT+ +AS A+L+G++ +TLTA+  +LHP  C        
Sbjct: 103 GFTNLTPLVGAYISDTYVGRFKTIAFASFATLLGLITITLTASFPQLHPASCNSQDPL-- 162

Query: 131 ACLEPTPWQIAFLLFGLGLLIIGAGGIRPCNLAFGADQFNPNTASGKLGINSFFNWYYFT 190
           +C  P   QI  LL GL  L +G+GGIRPC++ FG DQF+  T  G  G+ SFFNWYY T
Sbjct: 163 SCGGPNKLQIGVLLLGLCFLSVGSGGIRPCSIPFGVDQFDQRTEEGVKGVASFFNWYYMT 222

Query: 191 FTFAMIISLTIIVYVQTEVSWAWGLAIPAFLMFLSCALFFMGSWIYVKLEPQGSPFTSVM 250
           FT  +II+ T++VY+Q +VSW  G +IP  LM L+  +FF G   YV ++P+GS F+ + 
Sbjct: 223 FTVVLIITQTVVVYIQDQVSWIIGFSIPTGLMALAVVMFFAGMKRYVYVKPEGSIFSGIA 282

Query: 251 RVLVAALKKR--RLPSPDQQWPSLFNHIPSNSINSKLPYTDQFSFLNKAAIITPEDKFKS 310
           +V+VAA KKR  +LP+ D    + ++    +S+ SKL  ++QF  L+KAA++  E     
Sbjct: 283 QVIVAARKKRKLKLPAEDDGTVTYYDPAIKSSVLSKLHRSNQFRCLDKAAVVI-EGDLTP 342

Query: 311 DGSADDPWTLCSTQQVEEVKCLVRVIPIWGAAIMYHVATTQQQTYVVFQALQSDRRLFFG 370
           +G   D W LCS Q+VEEVKCL+R++PIW A I+   A T Q T+ V QAL+ DR L   
Sbjct: 343 EGPPADKWRLCSVQEVEEVKCLIRIVPIWSAGIISLAAMTTQGTFTVSQALKMDRNL--- 402

Query: 371 NTHYFKIPAASYTIFTMIGLTIWIPFYDRIVVPSLRRITSREGGITLLQKMGIGMVIAIV 430
               F+IPA S ++ +++ + I++PFYDR+ VP +RRIT  + GITLLQ++G G+V AI 
Sbjct: 403 -GPKFEIPAGSLSVISLLTIGIFLPFYDRVFVPFMRRITGHKSGITLLQRIGTGIVFAIF 462

Query: 431 TMFISALVEQKRRNLALTQPLCEETGRRAAISSMSALWLVPQLTLIGLSEAFTVIAQVEF 490
           +M ++ +VE+ RR  ++        G    ++ MS  WL PQL L+GL EAF +I Q+EF
Sbjct: 463 SMIVAGIVERMRRIRSI------NAGDPTGMTPMSVFWLSPQLILMGLCEAFNIIGQIEF 522

Query: 491 YYKEFPENMRSIGGSLSFVGLALSNYLSGFMVTVVHSLTTG----NWLPEDLNEGRLDYF 550
           +  +FPE+MRSI  SL  +  A S+YLS F+VTVVH  + G    +WL ++LN G+LDYF
Sbjct: 523 FNSQFPEHMRSIANSLFSLSFAGSSYLSSFLVTVVHKFSGGHDRPDWLNKNLNAGKLDYF 582

Query: 551 YFMVSGLEAVNLGYFVLCSKWYKYK 568
           Y++++ L  VNL YF  C++ Y+YK
Sbjct: 583 YYLIAVLGVVNLVYFWYCARGYRYK 594

BLAST of CmaCh10G001780 vs. ExPASy Swiss-Prot
Match: Q9LFX9 (Protein NRT1/ PTR FAMILY 2.12 OS=Arabidopsis thaliana OX=3702 GN=NPF2.12 PE=1 SV=2)

HSP 1 Score: 457.6 bits (1176), Expect = 2.1e-127
Identity = 247/554 (44.58%), Postives = 351/554 (63.36%), Query Frame = 0

Query: 20  GVKALPFVVGNETFEKLGTTGTSSNLLVYLTDVFHMKTITATTLMNIFHGSTNFSTLFGA 79
           G +A+ F++GNET EKLG+ G S+N ++YL +VFHM+ + A  +  ++ G TNF+ L GA
Sbjct: 17  GWRAITFILGNETLEKLGSIGVSANFMLYLRNVFHMEPVEAFNVYYLWMGLTNFAPLLGA 76

Query: 80  FLCDTYFGRYKTLGYASIASLMGMVVLTLTATIERLHPPDCGKGSAAGGACLEPTPWQIA 139
            + D Y GR+KT+ YAS+ S++G++ +TLTA + +LHPP C   +     C +P   Q+ 
Sbjct: 77  LISDAYIGRFKTIAYASLFSILGLMTVTLTACLPQLHPPPC--NNPHPDECDDPNKLQLG 136

Query: 140 FLLFGLGLLIIGAGGIRPCNLAFGADQFNPNTASGKLGINSFFNWYYFTFTFAMIISLTI 199
            L  GLG L IG+GGIRPC++ FG DQF+  T  G  G+ SFFNWYY T T  +I S T+
Sbjct: 137 ILFLGLGFLSIGSGGIRPCSIPFGVDQFDQRTEQGLKGVASFFNWYYLTLTMVLIFSHTV 196

Query: 200 IVYVQTEVSWAWGLAIPAFLMFLSCALFFMGSWIYVKLEPQGSPFTSVMRVLVAALKKR- 259
           +VY+QT VSW  G +IP  LM  +  LFF+G   YV ++P+GS F+ + RV+VAA KKR 
Sbjct: 197 VVYLQT-VSWVIGFSIPTSLMACAVVLFFVGMRFYVYVKPEGSVFSGIARVIVAARKKRD 256

Query: 260 -RLPSPDQQWPSLFNHIPSNSINSKLPYTDQFSFLNKAAIITPEDKFKSDGSADDPWTLC 319
            ++   D      +       + SKLP TDQF FL+KAA+I   D   S+G   + W LC
Sbjct: 257 LKISLVDDGTEEYYEPPVKPGVLSKLPLTDQFKFLDKAAVILDGD-LTSEGVPANKWRLC 316

Query: 320 STQQVEEVKCLVRVIPIWGAAIMYHVATTQQQTYVVFQALQSDRRLFFGNTHYFKIPAAS 379
           S Q+VEEVKCL+RV+P+W A I+  VA T Q T++VFQA + DR +      +F+IPAAS
Sbjct: 317 SIQEVEEVKCLIRVVPVWSAGIISIVAMTTQATFMVFQATKMDRHM----GPHFEIPAAS 376

Query: 380 YTIFTMIGLTIWIPFYDRIVVPSLRRITSREGGITLLQKMGIGMVIAIVTMFISALVEQK 439
            T+ + I + IW+P Y+ ++VP L R+  R+  +TLLQ+MGIG+V AI++MF +  VE  
Sbjct: 377 ITVISYITIGIWVPIYEHLLVPFLWRM--RKFRVTLLQRMGIGIVFAILSMFTAGFVEGV 436

Query: 440 RRNLALTQPLCEETGRRAAISSMSALWLVPQLTLIGLSEAFTVIAQVEFYYKEFPENMRS 499
           RR             R   ++ MS  WL   L L+GL E+F  I  +EF+  +FPE+MRS
Sbjct: 437 RRT------------RATEMTQMSVFWLALPLILMGLCESFNFIGLIEFFNSQFPEHMRS 496

Query: 500 IGGSLSFVGLALSNYLSGFMVTVVHSLT----TGNWLPEDLNEGRLDYFYFMVSGLEAVN 559
           I  SL  +  A +NYLS  +VT VH ++      +WL +DL+ G+LDYFY++++ L  VN
Sbjct: 497 IANSLFPLSFAAANYLSSLLVTTVHKVSGTKDHPDWLNKDLDRGKLDYFYYLIAVLGVVN 548

Query: 560 LGYFVLCSKWYKYK 568
           L YF  C+  Y+YK
Sbjct: 557 LVYFWYCAHRYQYK 548

BLAST of CmaCh10G001780 vs. ExPASy TrEMBL
Match: A0A6J1JCU2 (protein NRT1/ PTR FAMILY 2.10-like OS=Cucurbita maxima OX=3661 GN=LOC111485624 PE=3 SV=1)

HSP 1 Score: 1182.9 bits (3059), Expect = 0.0e+00
Identity = 590/590 (100.00%), Postives = 590/590 (100.00%), Query Frame = 0

Query: 1   MERNNEDVHNLDEAKVNYKGVKALPFVVGNETFEKLGTTGTSSNLLVYLTDVFHMKTITA 60
           MERNNEDVHNLDEAKVNYKGVKALPFVVGNETFEKLGTTGTSSNLLVYLTDVFHMKTITA
Sbjct: 1   MERNNEDVHNLDEAKVNYKGVKALPFVVGNETFEKLGTTGTSSNLLVYLTDVFHMKTITA 60

Query: 61  TTLMNIFHGSTNFSTLFGAFLCDTYFGRYKTLGYASIASLMGMVVLTLTATIERLHPPDC 120
           TTLMNIFHGSTNFSTLFGAFLCDTYFGRYKTLGYASIASLMGMVVLTLTATIERLHPPDC
Sbjct: 61  TTLMNIFHGSTNFSTLFGAFLCDTYFGRYKTLGYASIASLMGMVVLTLTATIERLHPPDC 120

Query: 121 GKGSAAGGACLEPTPWQIAFLLFGLGLLIIGAGGIRPCNLAFGADQFNPNTASGKLGINS 180
           GKGSAAGGACLEPTPWQIAFLLFGLGLLIIGAGGIRPCNLAFGADQFNPNTASGKLGINS
Sbjct: 121 GKGSAAGGACLEPTPWQIAFLLFGLGLLIIGAGGIRPCNLAFGADQFNPNTASGKLGINS 180

Query: 181 FFNWYYFTFTFAMIISLTIIVYVQTEVSWAWGLAIPAFLMFLSCALFFMGSWIYVKLEPQ 240
           FFNWYYFTFTFAMIISLTIIVYVQTEVSWAWGLAIPAFLMFLSCALFFMGSWIYVKLEPQ
Sbjct: 181 FFNWYYFTFTFAMIISLTIIVYVQTEVSWAWGLAIPAFLMFLSCALFFMGSWIYVKLEPQ 240

Query: 241 GSPFTSVMRVLVAALKKRRLPSPDQQWPSLFNHIPSNSINSKLPYTDQFSFLNKAAIITP 300
           GSPFTSVMRVLVAALKKRRLPSPDQQWPSLFNHIPSNSINSKLPYTDQFSFLNKAAIITP
Sbjct: 241 GSPFTSVMRVLVAALKKRRLPSPDQQWPSLFNHIPSNSINSKLPYTDQFSFLNKAAIITP 300

Query: 301 EDKFKSDGSADDPWTLCSTQQVEEVKCLVRVIPIWGAAIMYHVATTQQQTYVVFQALQSD 360
           EDKFKSDGSADDPWTLCSTQQVEEVKCLVRVIPIWGAAIMYHVATTQQQTYVVFQALQSD
Sbjct: 301 EDKFKSDGSADDPWTLCSTQQVEEVKCLVRVIPIWGAAIMYHVATTQQQTYVVFQALQSD 360

Query: 361 RRLFFGNTHYFKIPAASYTIFTMIGLTIWIPFYDRIVVPSLRRITSREGGITLLQKMGIG 420
           RRLFFGNTHYFKIPAASYTIFTMIGLTIWIPFYDRIVVPSLRRITSREGGITLLQKMGIG
Sbjct: 361 RRLFFGNTHYFKIPAASYTIFTMIGLTIWIPFYDRIVVPSLRRITSREGGITLLQKMGIG 420

Query: 421 MVIAIVTMFISALVEQKRRNLALTQPLCEETGRRAAISSMSALWLVPQLTLIGLSEAFTV 480
           MVIAIVTMFISALVEQKRRNLALTQPLCEETGRRAAISSMSALWLVPQLTLIGLSEAFTV
Sbjct: 421 MVIAIVTMFISALVEQKRRNLALTQPLCEETGRRAAISSMSALWLVPQLTLIGLSEAFTV 480

Query: 481 IAQVEFYYKEFPENMRSIGGSLSFVGLALSNYLSGFMVTVVHSLTTGNWLPEDLNEGRLD 540
           IAQVEFYYKEFPENMRSIGGSLSFVGLALSNYLSGFMVTVVHSLTTGNWLPEDLNEGRLD
Sbjct: 481 IAQVEFYYKEFPENMRSIGGSLSFVGLALSNYLSGFMVTVVHSLTTGNWLPEDLNEGRLD 540

Query: 541 YFYFMVSGLEAVNLGYFVLCSKWYKYKGSGSHGVDEMDFGKTEFEKTVVY 591
           YFYFMVSGLEAVNLGYFVLCSKWYKYKGSGSHGVDEMDFGKTEFEKTVVY
Sbjct: 541 YFYFMVSGLEAVNLGYFVLCSKWYKYKGSGSHGVDEMDFGKTEFEKTVVY 590

BLAST of CmaCh10G001780 vs. ExPASy TrEMBL
Match: A0A6J1E4K9 (protein NRT1/ PTR FAMILY 2.10-like OS=Cucurbita moschata OX=3662 GN=LOC111429868 PE=3 SV=1)

HSP 1 Score: 1174.8 bits (3038), Expect = 0.0e+00
Identity = 584/590 (98.98%), Postives = 587/590 (99.49%), Query Frame = 0

Query: 1   MERNNEDVHNLDEAKVNYKGVKALPFVVGNETFEKLGTTGTSSNLLVYLTDVFHMKTITA 60
           MERNNEDVHNLDEAKVNYKGVKALPFVVGNETFEKLGTTGTSSNLLVYLTDVFHMKTITA
Sbjct: 1   MERNNEDVHNLDEAKVNYKGVKALPFVVGNETFEKLGTTGTSSNLLVYLTDVFHMKTITA 60

Query: 61  TTLMNIFHGSTNFSTLFGAFLCDTYFGRYKTLGYASIASLMGMVVLTLTATIERLHPPDC 120
           TTLMNIFHGSTNFSTLFGAFLCDTYFGRYKTLGYASIASLMGMVVLTLTATIE+LHPPDC
Sbjct: 61  TTLMNIFHGSTNFSTLFGAFLCDTYFGRYKTLGYASIASLMGMVVLTLTATIEKLHPPDC 120

Query: 121 GKGSAAGGACLEPTPWQIAFLLFGLGLLIIGAGGIRPCNLAFGADQFNPNTASGKLGINS 180
           GKGSA GGACLEPTPWQIAFLLFGLGLLIIGAGGIRPCNLAFGADQFNPNTASGKLGINS
Sbjct: 121 GKGSATGGACLEPTPWQIAFLLFGLGLLIIGAGGIRPCNLAFGADQFNPNTASGKLGINS 180

Query: 181 FFNWYYFTFTFAMIISLTIIVYVQTEVSWAWGLAIPAFLMFLSCALFFMGSWIYVKLEPQ 240
           FFNWYYFTFTFAMIISLTIIVYVQTEVSWAWGLAIPAFLMFLSCALFFMGSWIYVKLEP+
Sbjct: 181 FFNWYYFTFTFAMIISLTIIVYVQTEVSWAWGLAIPAFLMFLSCALFFMGSWIYVKLEPE 240

Query: 241 GSPFTSVMRVLVAALKKRRLPSPDQQWPSLFNHIPSNSINSKLPYTDQFSFLNKAAIITP 300
           GSPFTSVMRVLVAALKKRRLPSPDQQWPSLFNHIPSNSINSKLPYTDQFSFLNKAAIITP
Sbjct: 241 GSPFTSVMRVLVAALKKRRLPSPDQQWPSLFNHIPSNSINSKLPYTDQFSFLNKAAIITP 300

Query: 301 EDKFKSDGSADDPWTLCSTQQVEEVKCLVRVIPIWGAAIMYHVATTQQQTYVVFQALQSD 360
           EDKFKSDGSADDPWTLCSTQQVEEVKCLVRVIPIWGAAIMYHVATTQQQTYVVFQALQSD
Sbjct: 301 EDKFKSDGSADDPWTLCSTQQVEEVKCLVRVIPIWGAAIMYHVATTQQQTYVVFQALQSD 360

Query: 361 RRLFFGNTHYFKIPAASYTIFTMIGLTIWIPFYDRIVVPSLRRITSREGGITLLQKMGIG 420
           RRLFFGNTHYFKIPAASYTIFTMIGLTIWIPFYDRIVVPSLRRIT+REGGITLLQKMGIG
Sbjct: 361 RRLFFGNTHYFKIPAASYTIFTMIGLTIWIPFYDRIVVPSLRRITAREGGITLLQKMGIG 420

Query: 421 MVIAIVTMFISALVEQKRRNLALTQPLCEETGRRAAISSMSALWLVPQLTLIGLSEAFTV 480
           MVIAIVTMFISALVEQKRRNLALTQPLCEETGRR AISSMSALWLVPQLTLIGLSEAFTV
Sbjct: 421 MVIAIVTMFISALVEQKRRNLALTQPLCEETGRRGAISSMSALWLVPQLTLIGLSEAFTV 480

Query: 481 IAQVEFYYKEFPENMRSIGGSLSFVGLALSNYLSGFMVTVVHSLTTGNWLPEDLNEGRLD 540
           IAQVEFYYKEFPENMRSIGGS SFVGLALSNYLSGFMVTVVHSLTTGNWLPEDLNEGRLD
Sbjct: 481 IAQVEFYYKEFPENMRSIGGSFSFVGLALSNYLSGFMVTVVHSLTTGNWLPEDLNEGRLD 540

Query: 541 YFYFMVSGLEAVNLGYFVLCSKWYKYKGSGSHGVDEMDFGKTEFEKTVVY 591
           YFYFMVSGLEAVNLGYFVLCSKWYKYKGSGSHGVDEMDFGKTEFEKTVVY
Sbjct: 541 YFYFMVSGLEAVNLGYFVLCSKWYKYKGSGSHGVDEMDFGKTEFEKTVVY 590

BLAST of CmaCh10G001780 vs. ExPASy TrEMBL
Match: A0A0A0LPB6 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G008540 PE=3 SV=1)

HSP 1 Score: 1119.4 bits (2894), Expect = 0.0e+00
Identity = 555/590 (94.07%), Postives = 573/590 (97.12%), Query Frame = 0

Query: 1   MERNNEDVHNLDEAKVNYKGVKALPFVVGNETFEKLGTTGTSSNLLVYLTDVFHMKTITA 60
           MERNNEDV+NLDE  VNY+GVKA+PFVVGNETFEKLGTTGTSSNLLVYLTDVFHMK ITA
Sbjct: 1   MERNNEDVNNLDEPNVNYRGVKAMPFVVGNETFEKLGTTGTSSNLLVYLTDVFHMKNITA 60

Query: 61  TTLMNIFHGSTNFSTLFGAFLCDTYFGRYKTLGYASIASLMGMVVLTLTATIERLHPPDC 120
           TTLMNIFHGSTNFSTLFGAFLCDTYFGRYKTLGYASIASLMGMVVLTLTATIE+LHPPDC
Sbjct: 61  TTLMNIFHGSTNFSTLFGAFLCDTYFGRYKTLGYASIASLMGMVVLTLTATIEKLHPPDC 120

Query: 121 GKGSAAGGACLEPTPWQIAFLLFGLGLLIIGAGGIRPCNLAFGADQFNPNTASGKLGINS 180
           GKGS AGGACLEPT WQIAFLLFGLGLL+IGAGGIRPCNLAFGADQFNPNTASGKLGINS
Sbjct: 121 GKGS-AGGACLEPTSWQIAFLLFGLGLLVIGAGGIRPCNLAFGADQFNPNTASGKLGINS 180

Query: 181 FFNWYYFTFTFAMIISLTIIVYVQTEVSWAWGLAIPAFLMFLSCALFFMGSWIYVKLEPQ 240
           FFNWYYFTFTFAM+ISLTIIVYVQTEVSWAWGLAIPAFLMFLSCALFFMGSWIYVKLEP 
Sbjct: 181 FFNWYYFTFTFAMMISLTIIVYVQTEVSWAWGLAIPAFLMFLSCALFFMGSWIYVKLEPD 240

Query: 241 GSPFTSVMRVLVAALKKRRLPSPDQQWPSLFNHIPSNSINSKLPYTDQFSFLNKAAIITP 300
           GSPFTSVMRVL+AALKKR+LP PDQQWPSLFNHIPSNSINSKLPYT+QFSF +KAAIIT 
Sbjct: 241 GSPFTSVMRVLMAALKKRQLPLPDQQWPSLFNHIPSNSINSKLPYTNQFSFFDKAAIITS 300

Query: 301 EDKFKSDGSADDPWTLCSTQQVEEVKCLVRVIPIWGAAIMYHVATTQQQTYVVFQALQSD 360
           EDKFKSDGSA DPWTLCSTQQVEEVKCLVRVIPIWGAAI+YHVATTQQQTY VFQALQSD
Sbjct: 301 EDKFKSDGSAADPWTLCSTQQVEEVKCLVRVIPIWGAAIIYHVATTQQQTYAVFQALQSD 360

Query: 361 RRLFFGNTHYFKIPAASYTIFTMIGLTIWIPFYDRIVVPSLRRITSREGGITLLQKMGIG 420
           RRLFFGNTHYFKIPAASYTIFTMIGLTIWIPFYDRI+VPSLRRIT+REGGITLLQKMG G
Sbjct: 361 RRLFFGNTHYFKIPAASYTIFTMIGLTIWIPFYDRILVPSLRRITAREGGITLLQKMGFG 420

Query: 421 MVIAIVTMFISALVEQKRRNLALTQPLCEETGRRAAISSMSALWLVPQLTLIGLSEAFTV 480
           M IAI+TMFISALVEQKRRNLALTQPLCEETGRR +ISSMSALWLVPQLTLIGLSEAFTV
Sbjct: 421 MGIAIITMFISALVEQKRRNLALTQPLCEETGRRGSISSMSALWLVPQLTLIGLSEAFTV 480

Query: 481 IAQVEFYYKEFPENMRSIGGSLSFVGLALSNYLSGFMVTVVHSLTTGNWLPEDLNEGRLD 540
           IAQVEFYYKEFPENMRSIGGSLSFVGLALSNYLSGFMVTVVH LT G WLPEDLN+GRLD
Sbjct: 481 IAQVEFYYKEFPENMRSIGGSLSFVGLALSNYLSGFMVTVVHRLTAGKWLPEDLNKGRLD 540

Query: 541 YFYFMVSGLEAVNLGYFVLCSKWYKYKGSGSHGVDEMDFGKTEFEKTVVY 591
           YFYF+VSGLEAVNLGYF+LCSKWYKYKGSGSHGVDEMDFGKTEFEKTVVY
Sbjct: 541 YFYFLVSGLEAVNLGYFLLCSKWYKYKGSGSHGVDEMDFGKTEFEKTVVY 589

BLAST of CmaCh10G001780 vs. ExPASy TrEMBL
Match: A0A5A7UPX7 (Protein NRT1/ PTR FAMILY 2.10-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold274G002260 PE=3 SV=1)

HSP 1 Score: 1111.3 bits (2873), Expect = 0.0e+00
Identity = 553/590 (93.73%), Postives = 570/590 (96.61%), Query Frame = 0

Query: 1   MERNNEDVHNLDEAKVNYKGVKALPFVVGNETFEKLGTTGTSSNLLVYLTDVFHMKTITA 60
           MERNNEDVHNLDE KVNYKGVKALPFVVGNETFEKLGTTGTSSNLLVYLTDVFHMK ITA
Sbjct: 1   MERNNEDVHNLDEPKVNYKGVKALPFVVGNETFEKLGTTGTSSNLLVYLTDVFHMKNITA 60

Query: 61  TTLMNIFHGSTNFSTLFGAFLCDTYFGRYKTLGYASIASLMGMVVLTLTATIERLHPPDC 120
           TTLMNIFHGSTNFSTLFGAFLCDTYFGRYKTLGYASIASLMGMVVLTLTATIE+LHPPDC
Sbjct: 61  TTLMNIFHGSTNFSTLFGAFLCDTYFGRYKTLGYASIASLMGMVVLTLTATIEKLHPPDC 120

Query: 121 GKGSAAGGACLEPTPWQIAFLLFGLGLLIIGAGGIRPCNLAFGADQFNPNTASGKLGINS 180
           GKGS AGGACLEPTPWQIAFLLFGLGLL+IGAGGIRPCNLAFGADQFNPNT SGKLGINS
Sbjct: 121 GKGS-AGGACLEPTPWQIAFLLFGLGLLVIGAGGIRPCNLAFGADQFNPNTVSGKLGINS 180

Query: 181 FFNWYYFTFTFAMIISLTIIVYVQTEVSWAWGLAIPAFLMFLSCALFFMGSWIYVKLEPQ 240
           FFNWYYFTF FAM+ISLTIIVYVQTEVSWAWGLAIPAFLMFLSCALFFMGSWIYVKLEP 
Sbjct: 181 FFNWYYFTFKFAMMISLTIIVYVQTEVSWAWGLAIPAFLMFLSCALFFMGSWIYVKLEPD 240

Query: 241 GSPFTSVMRVLVAALKKRRLPSPDQQWPSLFNHIPSNSINSKLPYTDQFSFLNKAAIITP 300
           GSPFTSV+RVL+AALKKR+LP PDQQW SLFNHIPSNSINSKLPYT+Q SFL+KAAIIT 
Sbjct: 241 GSPFTSVLRVLMAALKKRQLPLPDQQWSSLFNHIPSNSINSKLPYTNQLSFLDKAAIITS 300

Query: 301 EDKFKSDGSADDPWTLCSTQQVEEVKCLVRVIPIWGAAIMYHVATTQQQTYVVFQALQSD 360
           +DKFKSDGSA DPWTLCSTQQVEEVKCLVRVIPIW AAI+YHVATTQQQTY VFQALQSD
Sbjct: 301 DDKFKSDGSAADPWTLCSTQQVEEVKCLVRVIPIWCAAIIYHVATTQQQTYAVFQALQSD 360

Query: 361 RRLFFGNTHYFKIPAASYTIFTMIGLTIWIPFYDRIVVPSLRRITSREGGITLLQKMGIG 420
           RRLFFGNTHYFKIPAASYTIFTMIGLTIWIPFYDRI+VPSLRRIT+REGGITLLQKMG+G
Sbjct: 361 RRLFFGNTHYFKIPAASYTIFTMIGLTIWIPFYDRILVPSLRRITAREGGITLLQKMGLG 420

Query: 421 MVIAIVTMFISALVEQKRRNLALTQPLCEETGRRAAISSMSALWLVPQLTLIGLSEAFTV 480
           + IAI+TMFISALVEQKRRNLALTQPL EE GRR +ISSMSALWLVPQLTLIGLSEAFTV
Sbjct: 421 LGIAIITMFISALVEQKRRNLALTQPLYEERGRRGSISSMSALWLVPQLTLIGLSEAFTV 480

Query: 481 IAQVEFYYKEFPENMRSIGGSLSFVGLALSNYLSGFMVTVVHSLTTGNWLPEDLNEGRLD 540
           IAQVEFYYKEFPENMRSIGGSLSFVGLALSNYLSGFMVTVVH LT G WLPEDLNEGRLD
Sbjct: 481 IAQVEFYYKEFPENMRSIGGSLSFVGLALSNYLSGFMVTVVHRLTAGKWLPEDLNEGRLD 540

Query: 541 YFYFMVSGLEAVNLGYFVLCSKWYKYKGSGSHGVDEMDFGKTEFEKTVVY 591
           YFYF+VSGLEAVNLGYFVLCSKWYKYKGSGSHGVDEMDFGKTEFEKTVVY
Sbjct: 541 YFYFLVSGLEAVNLGYFVLCSKWYKYKGSGSHGVDEMDFGKTEFEKTVVY 589

BLAST of CmaCh10G001780 vs. ExPASy TrEMBL
Match: A0A5D3BIU4 (Protein NRT1/ PTR FAMILY 2.10-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold350G002340 PE=3 SV=1)

HSP 1 Score: 1110.5 bits (2871), Expect = 0.0e+00
Identity = 553/590 (93.73%), Postives = 570/590 (96.61%), Query Frame = 0

Query: 1   MERNNEDVHNLDEAKVNYKGVKALPFVVGNETFEKLGTTGTSSNLLVYLTDVFHMKTITA 60
           MERNNEDV NLDE KVNYKGVKALPFVVGNETFEKLGTTGTSSNLLVYLTDVFHMK ITA
Sbjct: 1   MERNNEDVRNLDEPKVNYKGVKALPFVVGNETFEKLGTTGTSSNLLVYLTDVFHMKNITA 60

Query: 61  TTLMNIFHGSTNFSTLFGAFLCDTYFGRYKTLGYASIASLMGMVVLTLTATIERLHPPDC 120
           TTLMNIFHGSTNFSTLFGAFLCDTYFGRYKTLGYASIASLMGMVVLTLTATIE+LHPPDC
Sbjct: 61  TTLMNIFHGSTNFSTLFGAFLCDTYFGRYKTLGYASIASLMGMVVLTLTATIEKLHPPDC 120

Query: 121 GKGSAAGGACLEPTPWQIAFLLFGLGLLIIGAGGIRPCNLAFGADQFNPNTASGKLGINS 180
           GKGS AGGACLEPTPWQIAFLLFGLGLL+IGAGGIRPCNLAFGADQFNPNT SGKLGINS
Sbjct: 121 GKGS-AGGACLEPTPWQIAFLLFGLGLLVIGAGGIRPCNLAFGADQFNPNTVSGKLGINS 180

Query: 181 FFNWYYFTFTFAMIISLTIIVYVQTEVSWAWGLAIPAFLMFLSCALFFMGSWIYVKLEPQ 240
           FFNWYYFTFTFAM+ISLTIIVYVQTEVSWAWGLAIPAFLMFLSCALFFMGSWIYVKLEP 
Sbjct: 181 FFNWYYFTFTFAMMISLTIIVYVQTEVSWAWGLAIPAFLMFLSCALFFMGSWIYVKLEPD 240

Query: 241 GSPFTSVMRVLVAALKKRRLPSPDQQWPSLFNHIPSNSINSKLPYTDQFSFLNKAAIITP 300
           GSPFTSV+RVL+AALKKR+LP PDQQW SLFNHIPSNSINSKLPYT+Q SFL+KAAIIT 
Sbjct: 241 GSPFTSVLRVLMAALKKRQLPLPDQQWSSLFNHIPSNSINSKLPYTNQLSFLDKAAIITS 300

Query: 301 EDKFKSDGSADDPWTLCSTQQVEEVKCLVRVIPIWGAAIMYHVATTQQQTYVVFQALQSD 360
           +DKFKSDGSA DPWTLCSTQQVEEVKCLVRVIPIW AAI+YHVATTQQQTY VFQALQSD
Sbjct: 301 DDKFKSDGSAADPWTLCSTQQVEEVKCLVRVIPIWCAAIIYHVATTQQQTYAVFQALQSD 360

Query: 361 RRLFFGNTHYFKIPAASYTIFTMIGLTIWIPFYDRIVVPSLRRITSREGGITLLQKMGIG 420
           RRLFFGNTHYFKIPAASYTIFTMIGLTIWIPFYDRI+VPSLRRIT+REGGITLLQKMG+G
Sbjct: 361 RRLFFGNTHYFKIPAASYTIFTMIGLTIWIPFYDRILVPSLRRITAREGGITLLQKMGLG 420

Query: 421 MVIAIVTMFISALVEQKRRNLALTQPLCEETGRRAAISSMSALWLVPQLTLIGLSEAFTV 480
           + IAI+TMFISALVEQKRRNLALTQPL EE GRR +ISSMSALWLVPQLTLIGLSEAFTV
Sbjct: 421 LGIAIITMFISALVEQKRRNLALTQPLYEERGRRGSISSMSALWLVPQLTLIGLSEAFTV 480

Query: 481 IAQVEFYYKEFPENMRSIGGSLSFVGLALSNYLSGFMVTVVHSLTTGNWLPEDLNEGRLD 540
           IAQVEFYYKEFPENMRSIGGSLSFVGLALSNYLSGFMVTVVH LT G WLPEDLNEGRLD
Sbjct: 481 IAQVEFYYKEFPENMRSIGGSLSFVGLALSNYLSGFMVTVVHRLTAGKWLPEDLNEGRLD 540

Query: 541 YFYFMVSGLEAVNLGYFVLCSKWYKYKGSGSHGVDEMDFGKTEFEKTVVY 591
           YFYF+VSGLEAVNLGYFVLCSKWYKYKGSGSHGVDEMDFGKTEFEKTVVY
Sbjct: 541 YFYFLVSGLEAVNLGYFVLCSKWYKYKGSGSHGVDEMDFGKTEFEKTVVY 589

BLAST of CmaCh10G001780 vs. NCBI nr
Match: XP_022988357.1 (protein NRT1/ PTR FAMILY 2.10-like [Cucurbita maxima])

HSP 1 Score: 1182.9 bits (3059), Expect = 0.0e+00
Identity = 590/590 (100.00%), Postives = 590/590 (100.00%), Query Frame = 0

Query: 1   MERNNEDVHNLDEAKVNYKGVKALPFVVGNETFEKLGTTGTSSNLLVYLTDVFHMKTITA 60
           MERNNEDVHNLDEAKVNYKGVKALPFVVGNETFEKLGTTGTSSNLLVYLTDVFHMKTITA
Sbjct: 1   MERNNEDVHNLDEAKVNYKGVKALPFVVGNETFEKLGTTGTSSNLLVYLTDVFHMKTITA 60

Query: 61  TTLMNIFHGSTNFSTLFGAFLCDTYFGRYKTLGYASIASLMGMVVLTLTATIERLHPPDC 120
           TTLMNIFHGSTNFSTLFGAFLCDTYFGRYKTLGYASIASLMGMVVLTLTATIERLHPPDC
Sbjct: 61  TTLMNIFHGSTNFSTLFGAFLCDTYFGRYKTLGYASIASLMGMVVLTLTATIERLHPPDC 120

Query: 121 GKGSAAGGACLEPTPWQIAFLLFGLGLLIIGAGGIRPCNLAFGADQFNPNTASGKLGINS 180
           GKGSAAGGACLEPTPWQIAFLLFGLGLLIIGAGGIRPCNLAFGADQFNPNTASGKLGINS
Sbjct: 121 GKGSAAGGACLEPTPWQIAFLLFGLGLLIIGAGGIRPCNLAFGADQFNPNTASGKLGINS 180

Query: 181 FFNWYYFTFTFAMIISLTIIVYVQTEVSWAWGLAIPAFLMFLSCALFFMGSWIYVKLEPQ 240
           FFNWYYFTFTFAMIISLTIIVYVQTEVSWAWGLAIPAFLMFLSCALFFMGSWIYVKLEPQ
Sbjct: 181 FFNWYYFTFTFAMIISLTIIVYVQTEVSWAWGLAIPAFLMFLSCALFFMGSWIYVKLEPQ 240

Query: 241 GSPFTSVMRVLVAALKKRRLPSPDQQWPSLFNHIPSNSINSKLPYTDQFSFLNKAAIITP 300
           GSPFTSVMRVLVAALKKRRLPSPDQQWPSLFNHIPSNSINSKLPYTDQFSFLNKAAIITP
Sbjct: 241 GSPFTSVMRVLVAALKKRRLPSPDQQWPSLFNHIPSNSINSKLPYTDQFSFLNKAAIITP 300

Query: 301 EDKFKSDGSADDPWTLCSTQQVEEVKCLVRVIPIWGAAIMYHVATTQQQTYVVFQALQSD 360
           EDKFKSDGSADDPWTLCSTQQVEEVKCLVRVIPIWGAAIMYHVATTQQQTYVVFQALQSD
Sbjct: 301 EDKFKSDGSADDPWTLCSTQQVEEVKCLVRVIPIWGAAIMYHVATTQQQTYVVFQALQSD 360

Query: 361 RRLFFGNTHYFKIPAASYTIFTMIGLTIWIPFYDRIVVPSLRRITSREGGITLLQKMGIG 420
           RRLFFGNTHYFKIPAASYTIFTMIGLTIWIPFYDRIVVPSLRRITSREGGITLLQKMGIG
Sbjct: 361 RRLFFGNTHYFKIPAASYTIFTMIGLTIWIPFYDRIVVPSLRRITSREGGITLLQKMGIG 420

Query: 421 MVIAIVTMFISALVEQKRRNLALTQPLCEETGRRAAISSMSALWLVPQLTLIGLSEAFTV 480
           MVIAIVTMFISALVEQKRRNLALTQPLCEETGRRAAISSMSALWLVPQLTLIGLSEAFTV
Sbjct: 421 MVIAIVTMFISALVEQKRRNLALTQPLCEETGRRAAISSMSALWLVPQLTLIGLSEAFTV 480

Query: 481 IAQVEFYYKEFPENMRSIGGSLSFVGLALSNYLSGFMVTVVHSLTTGNWLPEDLNEGRLD 540
           IAQVEFYYKEFPENMRSIGGSLSFVGLALSNYLSGFMVTVVHSLTTGNWLPEDLNEGRLD
Sbjct: 481 IAQVEFYYKEFPENMRSIGGSLSFVGLALSNYLSGFMVTVVHSLTTGNWLPEDLNEGRLD 540

Query: 541 YFYFMVSGLEAVNLGYFVLCSKWYKYKGSGSHGVDEMDFGKTEFEKTVVY 591
           YFYFMVSGLEAVNLGYFVLCSKWYKYKGSGSHGVDEMDFGKTEFEKTVVY
Sbjct: 541 YFYFMVSGLEAVNLGYFVLCSKWYKYKGSGSHGVDEMDFGKTEFEKTVVY 590

BLAST of CmaCh10G001780 vs. NCBI nr
Match: XP_023515678.1 (protein NRT1/ PTR FAMILY 2.10-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1176.0 bits (3041), Expect = 0.0e+00
Identity = 584/590 (98.98%), Postives = 588/590 (99.66%), Query Frame = 0

Query: 1   MERNNEDVHNLDEAKVNYKGVKALPFVVGNETFEKLGTTGTSSNLLVYLTDVFHMKTITA 60
           MERNNED+HNLDEAKVNYKGVKALPFVVGNETFEKLGTTGTSSNLLVYLTDVFHMKTITA
Sbjct: 1   MERNNEDIHNLDEAKVNYKGVKALPFVVGNETFEKLGTTGTSSNLLVYLTDVFHMKTITA 60

Query: 61  TTLMNIFHGSTNFSTLFGAFLCDTYFGRYKTLGYASIASLMGMVVLTLTATIERLHPPDC 120
           TTLMNIFHGSTNFSTLFGAFLCDTYFGRYKTLGYASIASLMGMVVLTLTATIE+LHPPDC
Sbjct: 61  TTLMNIFHGSTNFSTLFGAFLCDTYFGRYKTLGYASIASLMGMVVLTLTATIEKLHPPDC 120

Query: 121 GKGSAAGGACLEPTPWQIAFLLFGLGLLIIGAGGIRPCNLAFGADQFNPNTASGKLGINS 180
           GKGSAAGGACLEPTPWQIAFLLFGLGLLIIGAGGIRPCNLAFGADQFNPNTASGKLGINS
Sbjct: 121 GKGSAAGGACLEPTPWQIAFLLFGLGLLIIGAGGIRPCNLAFGADQFNPNTASGKLGINS 180

Query: 181 FFNWYYFTFTFAMIISLTIIVYVQTEVSWAWGLAIPAFLMFLSCALFFMGSWIYVKLEPQ 240
           FFNWYYFTFTFAMIISLTIIVYVQTEVSWAWGLAIPAFLMFLSCALFFMGSWIYVKLEP+
Sbjct: 181 FFNWYYFTFTFAMIISLTIIVYVQTEVSWAWGLAIPAFLMFLSCALFFMGSWIYVKLEPE 240

Query: 241 GSPFTSVMRVLVAALKKRRLPSPDQQWPSLFNHIPSNSINSKLPYTDQFSFLNKAAIITP 300
           GSPFTSVMRVLVAALKKRRLPSPDQQWPSLFNHIPSNSINSKLPYTDQFSFLNKAAIITP
Sbjct: 241 GSPFTSVMRVLVAALKKRRLPSPDQQWPSLFNHIPSNSINSKLPYTDQFSFLNKAAIITP 300

Query: 301 EDKFKSDGSADDPWTLCSTQQVEEVKCLVRVIPIWGAAIMYHVATTQQQTYVVFQALQSD 360
           EDKFKSDGSADDPWTLCSTQQVEEVKCLVRVIPIWGAAIMYHVATTQQQTYVVFQALQSD
Sbjct: 301 EDKFKSDGSADDPWTLCSTQQVEEVKCLVRVIPIWGAAIMYHVATTQQQTYVVFQALQSD 360

Query: 361 RRLFFGNTHYFKIPAASYTIFTMIGLTIWIPFYDRIVVPSLRRITSREGGITLLQKMGIG 420
           RRLFFGNTHYFKIPAASYTIFTMIGLTIWIPFYDRIVVPSLRRIT+REGGITLLQKMGIG
Sbjct: 361 RRLFFGNTHYFKIPAASYTIFTMIGLTIWIPFYDRIVVPSLRRITAREGGITLLQKMGIG 420

Query: 421 MVIAIVTMFISALVEQKRRNLALTQPLCEETGRRAAISSMSALWLVPQLTLIGLSEAFTV 480
           MVIAIVTMFISALVEQKRRNLALTQPLCEETGRR AISSMSALWLVPQLTLIGLSEAFTV
Sbjct: 421 MVIAIVTMFISALVEQKRRNLALTQPLCEETGRRGAISSMSALWLVPQLTLIGLSEAFTV 480

Query: 481 IAQVEFYYKEFPENMRSIGGSLSFVGLALSNYLSGFMVTVVHSLTTGNWLPEDLNEGRLD 540
           IAQVEFYYKEFPENMRSIGGS SFVGLALSNYLSGFMVTVVHSLTTGNWLPEDLNEGRLD
Sbjct: 481 IAQVEFYYKEFPENMRSIGGSFSFVGLALSNYLSGFMVTVVHSLTTGNWLPEDLNEGRLD 540

Query: 541 YFYFMVSGLEAVNLGYFVLCSKWYKYKGSGSHGVDEMDFGKTEFEKTVVY 591
           YFYFMVSGLEAVNLGYFVLCSKWYKYKGSGSHGVDEMDFGKTEFEKTVVY
Sbjct: 541 YFYFMVSGLEAVNLGYFVLCSKWYKYKGSGSHGVDEMDFGKTEFEKTVVY 590

BLAST of CmaCh10G001780 vs. NCBI nr
Match: XP_022921698.1 (protein NRT1/ PTR FAMILY 2.10-like [Cucurbita moschata])

HSP 1 Score: 1174.8 bits (3038), Expect = 0.0e+00
Identity = 584/590 (98.98%), Postives = 587/590 (99.49%), Query Frame = 0

Query: 1   MERNNEDVHNLDEAKVNYKGVKALPFVVGNETFEKLGTTGTSSNLLVYLTDVFHMKTITA 60
           MERNNEDVHNLDEAKVNYKGVKALPFVVGNETFEKLGTTGTSSNLLVYLTDVFHMKTITA
Sbjct: 1   MERNNEDVHNLDEAKVNYKGVKALPFVVGNETFEKLGTTGTSSNLLVYLTDVFHMKTITA 60

Query: 61  TTLMNIFHGSTNFSTLFGAFLCDTYFGRYKTLGYASIASLMGMVVLTLTATIERLHPPDC 120
           TTLMNIFHGSTNFSTLFGAFLCDTYFGRYKTLGYASIASLMGMVVLTLTATIE+LHPPDC
Sbjct: 61  TTLMNIFHGSTNFSTLFGAFLCDTYFGRYKTLGYASIASLMGMVVLTLTATIEKLHPPDC 120

Query: 121 GKGSAAGGACLEPTPWQIAFLLFGLGLLIIGAGGIRPCNLAFGADQFNPNTASGKLGINS 180
           GKGSA GGACLEPTPWQIAFLLFGLGLLIIGAGGIRPCNLAFGADQFNPNTASGKLGINS
Sbjct: 121 GKGSATGGACLEPTPWQIAFLLFGLGLLIIGAGGIRPCNLAFGADQFNPNTASGKLGINS 180

Query: 181 FFNWYYFTFTFAMIISLTIIVYVQTEVSWAWGLAIPAFLMFLSCALFFMGSWIYVKLEPQ 240
           FFNWYYFTFTFAMIISLTIIVYVQTEVSWAWGLAIPAFLMFLSCALFFMGSWIYVKLEP+
Sbjct: 181 FFNWYYFTFTFAMIISLTIIVYVQTEVSWAWGLAIPAFLMFLSCALFFMGSWIYVKLEPE 240

Query: 241 GSPFTSVMRVLVAALKKRRLPSPDQQWPSLFNHIPSNSINSKLPYTDQFSFLNKAAIITP 300
           GSPFTSVMRVLVAALKKRRLPSPDQQWPSLFNHIPSNSINSKLPYTDQFSFLNKAAIITP
Sbjct: 241 GSPFTSVMRVLVAALKKRRLPSPDQQWPSLFNHIPSNSINSKLPYTDQFSFLNKAAIITP 300

Query: 301 EDKFKSDGSADDPWTLCSTQQVEEVKCLVRVIPIWGAAIMYHVATTQQQTYVVFQALQSD 360
           EDKFKSDGSADDPWTLCSTQQVEEVKCLVRVIPIWGAAIMYHVATTQQQTYVVFQALQSD
Sbjct: 301 EDKFKSDGSADDPWTLCSTQQVEEVKCLVRVIPIWGAAIMYHVATTQQQTYVVFQALQSD 360

Query: 361 RRLFFGNTHYFKIPAASYTIFTMIGLTIWIPFYDRIVVPSLRRITSREGGITLLQKMGIG 420
           RRLFFGNTHYFKIPAASYTIFTMIGLTIWIPFYDRIVVPSLRRIT+REGGITLLQKMGIG
Sbjct: 361 RRLFFGNTHYFKIPAASYTIFTMIGLTIWIPFYDRIVVPSLRRITAREGGITLLQKMGIG 420

Query: 421 MVIAIVTMFISALVEQKRRNLALTQPLCEETGRRAAISSMSALWLVPQLTLIGLSEAFTV 480
           MVIAIVTMFISALVEQKRRNLALTQPLCEETGRR AISSMSALWLVPQLTLIGLSEAFTV
Sbjct: 421 MVIAIVTMFISALVEQKRRNLALTQPLCEETGRRGAISSMSALWLVPQLTLIGLSEAFTV 480

Query: 481 IAQVEFYYKEFPENMRSIGGSLSFVGLALSNYLSGFMVTVVHSLTTGNWLPEDLNEGRLD 540
           IAQVEFYYKEFPENMRSIGGS SFVGLALSNYLSGFMVTVVHSLTTGNWLPEDLNEGRLD
Sbjct: 481 IAQVEFYYKEFPENMRSIGGSFSFVGLALSNYLSGFMVTVVHSLTTGNWLPEDLNEGRLD 540

Query: 541 YFYFMVSGLEAVNLGYFVLCSKWYKYKGSGSHGVDEMDFGKTEFEKTVVY 591
           YFYFMVSGLEAVNLGYFVLCSKWYKYKGSGSHGVDEMDFGKTEFEKTVVY
Sbjct: 541 YFYFMVSGLEAVNLGYFVLCSKWYKYKGSGSHGVDEMDFGKTEFEKTVVY 590

BLAST of CmaCh10G001780 vs. NCBI nr
Match: KAG7023179.1 (Protein NRT1/ PTR FAMILY 2.11, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1172.5 bits (3032), Expect = 0.0e+00
Identity = 584/590 (98.98%), Postives = 587/590 (99.49%), Query Frame = 0

Query: 1   MERNNEDVHNLDEAKVNYKGVKALPFVVGNETFEKLGTTGTSSNLLVYLTDVFHMKTITA 60
           MERNNEDVHNLDEAKVNYKGVKALPFVVGNETFEKLGTTGTSSNLLVYLTDVFHMKTITA
Sbjct: 1   MERNNEDVHNLDEAKVNYKGVKALPFVVGNETFEKLGTTGTSSNLLVYLTDVFHMKTITA 60

Query: 61  TTLMNIFHGSTNFSTLFGAFLCDTYFGRYKTLGYASIASLMGMVVLTLTATIERLHPPDC 120
           TTLMNIFHGSTNFSTLFGAFLCDTYFGRYKTLGYASIASLMGMVVLTLTATIE+LHPPDC
Sbjct: 61  TTLMNIFHGSTNFSTLFGAFLCDTYFGRYKTLGYASIASLMGMVVLTLTATIEKLHPPDC 120

Query: 121 GKGSAAGGACLEPTPWQIAFLLFGLGLLIIGAGGIRPCNLAFGADQFNPNTASGKLGINS 180
           GKGSAAGGACLEPTPWQIAFLLFGLGLLIIGAGGIRPCNLAFGADQFNPNTASGKLGINS
Sbjct: 121 GKGSAAGGACLEPTPWQIAFLLFGLGLLIIGAGGIRPCNLAFGADQFNPNTASGKLGINS 180

Query: 181 FFNWYYFTFTFAMIISLTIIVYVQTEVSWAWGLAIPAFLMFLSCALFFMGSWIYVKLEPQ 240
           FFNWYYFTFTFAMIISLTIIVYVQTEVSWAWGLAIPAFLMFLSCALFFMGSWIYVKLEP+
Sbjct: 181 FFNWYYFTFTFAMIISLTIIVYVQTEVSWAWGLAIPAFLMFLSCALFFMGSWIYVKLEPE 240

Query: 241 GSPFTSVMRVLVAALKKRRLPSPDQQWPSLFNHIPSNSINSKLPYTDQFSFLNKAAIITP 300
           GSPFTSVMRVLVAALKKRRLPSPDQQWPSLFNHIPSNSINSKLPYTDQFSFLNKAAIITP
Sbjct: 241 GSPFTSVMRVLVAALKKRRLPSPDQQWPSLFNHIPSNSINSKLPYTDQFSFLNKAAIITP 300

Query: 301 EDKFKSDGSADDPWTLCSTQQVEEVKCLVRVIPIWGAAIMYHVATTQQQTYVVFQALQSD 360
           EDKFKSDGSADDPWTLCSTQQVEEVKCLVRVIPIWGAAIMYHVATTQQQTYVVFQALQSD
Sbjct: 301 EDKFKSDGSADDPWTLCSTQQVEEVKCLVRVIPIWGAAIMYHVATTQQQTYVVFQALQSD 360

Query: 361 RRLFFGNTHYFKIPAASYTIFTMIGLTIWIPFYDRIVVPSLRRITSREGGITLLQKMGIG 420
           RRLFFGNTHYFKIPAASYTIFTMIGLTIWIPFYDRIVVPSLRRIT+REGGITLLQKMGIG
Sbjct: 361 RRLFFGNTHYFKIPAASYTIFTMIGLTIWIPFYDRIVVPSLRRITAREGGITLLQKMGIG 420

Query: 421 MVIAIVTMFISALVEQKRRNLALTQPLCEETGRRAAISSMSALWLVPQLTLIGLSEAFTV 480
           MVIAIVTMFISALVEQKRRNLALTQPL EETGRR AISSMSALWLVPQLTLIGLSEAFTV
Sbjct: 421 MVIAIVTMFISALVEQKRRNLALTQPLSEETGRRGAISSMSALWLVPQLTLIGLSEAFTV 480

Query: 481 IAQVEFYYKEFPENMRSIGGSLSFVGLALSNYLSGFMVTVVHSLTTGNWLPEDLNEGRLD 540
           IAQVEFYYKEFPENMRSIGGS SFVGLALSNYLSGFMVTVVHSLTTGNWLPEDLNEGRLD
Sbjct: 481 IAQVEFYYKEFPENMRSIGGSFSFVGLALSNYLSGFMVTVVHSLTTGNWLPEDLNEGRLD 540

Query: 541 YFYFMVSGLEAVNLGYFVLCSKWYKYKGSGSHGVDEMDFGKTEFEKTVVY 591
           YFYFMVSGLEAVNLGYFVLCSKWYKYKGSGSHGVDEMDFGKTEFEKTVVY
Sbjct: 541 YFYFMVSGLEAVNLGYFVLCSKWYKYKGSGSHGVDEMDFGKTEFEKTVVY 590

BLAST of CmaCh10G001780 vs. NCBI nr
Match: KAG6589494.1 (Protein NRT1/ PTR FAMILY 2.11, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1167.9 bits (3020), Expect = 0.0e+00
Identity = 582/590 (98.64%), Postives = 585/590 (99.15%), Query Frame = 0

Query: 1   MERNNEDVHNLDEAKVNYKGVKALPFVVGNETFEKLGTTGTSSNLLVYLTDVFHMKTITA 60
           MERNNEDVHNLDEAKVNYKGVKALPFVVGNETFEKLGTTGTSSNLLVYLTDVFHMKTITA
Sbjct: 1   MERNNEDVHNLDEAKVNYKGVKALPFVVGNETFEKLGTTGTSSNLLVYLTDVFHMKTITA 60

Query: 61  TTLMNIFHGSTNFSTLFGAFLCDTYFGRYKTLGYASIASLMGMVVLTLTATIERLHPPDC 120
           TTLMNIFHGSTNFSTLFGAFLCDTYFGRYKTLGYASIASLMGMVVLTLTATIE+LHPPDC
Sbjct: 61  TTLMNIFHGSTNFSTLFGAFLCDTYFGRYKTLGYASIASLMGMVVLTLTATIEKLHPPDC 120

Query: 121 GKGSAAGGACLEPTPWQIAFLLFGLGLLIIGAGGIRPCNLAFGADQFNPNTASGKLGINS 180
           GKGSA GGACLEPTPWQIAFLLFGLGLLIIGAGGIRPCNLAFGADQFNPNTASGKLGINS
Sbjct: 121 GKGSATGGACLEPTPWQIAFLLFGLGLLIIGAGGIRPCNLAFGADQFNPNTASGKLGINS 180

Query: 181 FFNWYYFTFTFAMIISLTIIVYVQTEVSWAWGLAIPAFLMFLSCALFFMGSWIYVKLEPQ 240
           FFNWYYFTFTFAMIISLTIIVYVQTEVSWAWGLAIPAFLMFLSCALFFMGSWIYVKLE +
Sbjct: 181 FFNWYYFTFTFAMIISLTIIVYVQTEVSWAWGLAIPAFLMFLSCALFFMGSWIYVKLEAE 240

Query: 241 GSPFTSVMRVLVAALKKRRLPSPDQQWPSLFNHIPSNSINSKLPYTDQFSFLNKAAIITP 300
           GSPFTSVMRVLVAALKKRRLPSPDQQWPSLFNHIPSNSINSKLPYTDQFSFLNKAAIITP
Sbjct: 241 GSPFTSVMRVLVAALKKRRLPSPDQQWPSLFNHIPSNSINSKLPYTDQFSFLNKAAIITP 300

Query: 301 EDKFKSDGSADDPWTLCSTQQVEEVKCLVRVIPIWGAAIMYHVATTQQQTYVVFQALQSD 360
           EDKFKSDGSADDPWTLCSTQQVEEVKCLVRVIPIWGAAIMYHVATTQQQTYVVFQALQSD
Sbjct: 301 EDKFKSDGSADDPWTLCSTQQVEEVKCLVRVIPIWGAAIMYHVATTQQQTYVVFQALQSD 360

Query: 361 RRLFFGNTHYFKIPAASYTIFTMIGLTIWIPFYDRIVVPSLRRITSREGGITLLQKMGIG 420
           RRLFFGNTHYFKIPAASYTIFTMIGLTIWIPFYDRIVVPSLRRIT+REGGITLLQKMGIG
Sbjct: 361 RRLFFGNTHYFKIPAASYTIFTMIGLTIWIPFYDRIVVPSLRRITAREGGITLLQKMGIG 420

Query: 421 MVIAIVTMFISALVEQKRRNLALTQPLCEETGRRAAISSMSALWLVPQLTLIGLSEAFTV 480
           MVIAIVTMFISALVEQKRRNLALTQPL EETGRR AISSMSALWLVPQLTLIGLSEAFTV
Sbjct: 421 MVIAIVTMFISALVEQKRRNLALTQPLSEETGRRGAISSMSALWLVPQLTLIGLSEAFTV 480

Query: 481 IAQVEFYYKEFPENMRSIGGSLSFVGLALSNYLSGFMVTVVHSLTTGNWLPEDLNEGRLD 540
           IAQVEFYYKEFPENMRSIGGS SFVGLALSNYLSGFMVTVVHSLTTGNWLPEDLNEGRLD
Sbjct: 481 IAQVEFYYKEFPENMRSIGGSFSFVGLALSNYLSGFMVTVVHSLTTGNWLPEDLNEGRLD 540

Query: 541 YFYFMVSGLEAVNLGYFVLCSKWYKYKGSGSHGVDEMDFGKTEFEKTVVY 591
           YFYFMVSGLEAVNLGYFVLCSKWYKYKGSGSHGVDEMDFGKTEFEKTVVY
Sbjct: 541 YFYFMVSGLEAVNLGYFVLCSKWYKYKGSGSHGVDEMDFGKTEFEKTVVY 590

BLAST of CmaCh10G001780 vs. TAIR 10
Match: AT3G47960.1 (Major facilitator superfamily protein )

HSP 1 Score: 682.2 bits (1759), Expect = 3.8e-196
Identity = 332/576 (57.64%), Postives = 425/576 (73.78%), Query Frame = 0

Query: 12  DEAKVNYKGVKALPFVVGNETFEKLGTTGTSSNLLVYLTDVFHMKTITATTLMNIFHGST 71
           ++ K+ Y+G K +PF++GNETFEKLG  GT SNLLVYLT VF++K+ TA T++N F G+ 
Sbjct: 56  EQRKIVYRGWKVMPFIIGNETFEKLGIIGTLSNLLVYLTSVFNLKSYTAATIINAFSGTI 115

Query: 72  NFSTLFGAFLCDTYFGRYKTLGYASIASLMGMVVLTLTATIERLHPPDCGKGSAAGGACL 131
           NF T   AFLCDTYFGRYKTL  A IA  +G  V+ LTA I  LHP  CG       +C 
Sbjct: 116 NFGTFIAAFLCDTYFGRYKTLSVAVIACFLGSFVILLTAAIPSLHPVACGNKI----SCE 175

Query: 132 EPTPWQIAFLLFGLGLLIIGAGGIRPCNLAFGADQFNPNTASGKLGINSFFNWYYFTFTF 191
            P+  QI FLL GLG L++GAGGIRPCNLAFGADQFNP + SGK GINSFFNWY+FTFTF
Sbjct: 176 GPSVGQILFLLMGLGFLVVGAGGIRPCNLAFGADQFNPKSESGKKGINSFFNWYFFTFTF 235

Query: 192 AMIISLTIIVYVQTEVSWAWGLAIPAFLMFLSCALFFMGSWIYVKLEPQGSPFTSVMRVL 251
           A IISLT +VY+Q+ VSW  GL IP  LMFL+C +FF G  +YVK++  GSP   + RV+
Sbjct: 236 AQIISLTAVVYIQSNVSWTIGLIIPVALMFLACVIFFAGDRLYVKVKASGSPLAGIARVI 295

Query: 252 VAALKKRRLPSPDQQWPSLFNHIPSNSINSKLPYTDQFSFLNKAAIITPEDKFKSDGSAD 311
            AA+KKR L    Q W +L+NHIPSN  N+ L YTDQF FL+KAAI+TPE+K  SDG+A 
Sbjct: 296 AAAIKKRGLKPVKQPWVNLYNHIPSNYANTTLKYTDQFRFLDKAAIMTPEEKLNSDGTAS 355

Query: 312 DPWTLCSTQQVEEVKCLVRVIPIWGAAIMYHVATTQQQTYVVFQALQSDRRLFFGNTHYF 371
           DPW LC+ QQVEEVKC+VRVIPIW A+ +Y++A T Q TY VFQALQSDRRL  G    F
Sbjct: 356 DPWKLCTLQQVEEVKCIVRVIPIWFASTIYYLAITIQMTYPVFQALQSDRRLGSGG---F 415

Query: 372 KIPAASYTIFTMIGLTIWIPFYDRIVVPSLRRITSREGGITLLQKMGIGMVIAIVTMFIS 431
           +IPAA+Y +F M G+T++I FYDR++VPSLRR+T  E GI+LLQ++G G   AI+++ +S
Sbjct: 416 RIPAATYVVFLMTGMTVFIIFYDRVLVPSLRRVTGLETGISLLQRIGAGFTFAIMSLLVS 475

Query: 432 ALVEQKRRNLALTQPLCEETGRRAAISSMSALWLVPQLTLIGLSEAFTVIAQVEFYYKEF 491
             +E++RRN ALT+P      R   ISSMSALWL+PQLTL G++EAF  I Q+EFYYK+F
Sbjct: 476 GFIEERRRNFALTKPTLGMAPRTGEISSMSALWLIPQLTLAGIAEAFAAIGQMEFYYKQF 535

Query: 492 PENMRSIGGSLSFVGLALSNYLSGFMVTVVHSLT----TGNWLPEDLNEGRLDYFYFMVS 551
           PENM+S  GS+ +VG  +S+YL+ F+++ VH  T    +GNWL EDLN+ +LDYFYFM++
Sbjct: 536 PENMKSFAGSIFYVGAGVSSYLASFLISTVHRTTAHSPSGNWLAEDLNKAKLDYFYFMLT 595

Query: 552 GLEAVNLGYFVLCSKWYKYKGSGSHGVDEMDFGKTE 584
           GL  VN+ YF+L ++WY+YKG     + E++  + E
Sbjct: 596 GLMVVNMAYFLLMARWYRYKGGNDEDITEIETNEEE 624

BLAST of CmaCh10G001780 vs. TAIR 10
Match: AT5G62680.1 (Major facilitator superfamily protein )

HSP 1 Score: 666.4 bits (1718), Expect = 2.1e-191
Identity = 329/568 (57.92%), Postives = 419/568 (73.77%), Query Frame = 0

Query: 6   EDVHNLDEAKVNYKGVKALPFVVGNETFEKLGTTGTSSNLLVYLTDVFHMKTITATTLMN 65
           EDV N  + KV Y+G K +PF++GNETFEKLG  GT SNLLVYLT VF++K+ITA T++N
Sbjct: 34  EDVQN--QKKVVYRGWKVMPFIIGNETFEKLGIIGTLSNLLVYLTAVFNLKSITAATIIN 93

Query: 66  IFHGSTNFSTLFGAFLCDTYFGRYKTLGYASIASLMGMVVLTLTATIERLHPPDCGKGSA 125
            F G+ NF T   AFLCDTYFGRYKTL  A IA  +G  V+ LTA + +LHP  C  G+A
Sbjct: 94  AFSGTINFGTFVAAFLCDTYFGRYKTLSVAVIACFLGSFVILLTAAVPQLHPAAC--GTA 153

Query: 126 AGGACLEPTPWQIAFLLFGLGLLIIGAGGIRPCNLAFGADQFNPNTASGKLGINSFFNWY 185
           A   C  P+  QIAFLL GLG L++GAGGIRPCNLAFGADQFNP + SGK GI+SFFNWY
Sbjct: 154 ADSICNGPSGGQIAFLLMGLGFLVVGAGGIRPCNLAFGADQFNPKSESGKRGIDSFFNWY 213

Query: 186 YFTFTFAMIISLTIIVYVQTEVSWAWGLAIPAFLMFLSCALFFMGSWIYVKLEPQGSPFT 245
           +FTFTFA I+SLT++VYVQ+ VSW  GL IPA LMFL+C +FF G  +YVK++  GSP  
Sbjct: 214 FFTFTFAQILSLTLVVYVQSNVSWTIGLTIPAVLMFLACLIFFAGDKLYVKIKASGSPLA 273

Query: 246 SVMRVLVAALKKRRLPSPDQQWPSLFNHIPSNSINSKLPYTDQFSFLNKAAIITPEDKFK 305
            + +V+  A+KKR L    Q W +L+N+ P    NSKL YTDQF FL+KAAI+TPEDK +
Sbjct: 274 GIAQVIAVAIKKRGLKPAKQPWLNLYNYYPPKYANSKLKYTDQFRFLDKAAILTPEDKLQ 333

Query: 306 SDGSADDPWTLCSTQQVEEVKCLVRVIPIWGAAIMYHVATTQQQTYVVFQALQSDRRLFF 365
            DG   DPW LC+ QQVEEVKC+VRV+PIW A+ +Y++  TQQ TY VFQALQSDRRL  
Sbjct: 334 PDGKPADPWKLCTMQQVEEVKCIVRVLPIWFASSIYYLTITQQMTYPVFQALQSDRRLGS 393

Query: 366 GNTHYFKIPAASYTIFTMIGLTIWIPFYDRIVVPSLRRITSREGGITLLQKMGIGMVIAI 425
           G    F IPAA+Y +F M G+T++I  YDR++VP++RRIT  + GITLLQ++G G+  A 
Sbjct: 394 GG---FVIPAATYVVFLMTGMTVFIVVYDRVLVPTMRRITGLDTGITLLQRIGTGIFFAT 453

Query: 426 VTMFISALVEQKRRNLALTQPLCEETGRRAAISSMSALWLVPQLTLIGLSEAFTVIAQVE 485
            ++ ++  VE++RR  ALT+P      R+  ISSMSA+WL+PQL+L G++EAF  I Q+E
Sbjct: 454 ASLVVAGFVEERRRTFALTKPTLGMAPRKGEISSMSAMWLIPQLSLAGVAEAFAAIGQME 513

Query: 486 FYYKEFPENMRSIGGSLSFVGLALSNYLSGFMVTVVHSLTT----GNWLPEDLNEGRLDY 545
           FYYK+FPENMRS  GS+ +VG  +S+YL  F++  VH  T     GNWL EDLN+GRLD 
Sbjct: 514 FYYKQFPENMRSFAGSIFYVGGGVSSYLGSFLIATVHRTTQNSSGGNWLAEDLNKGRLDL 573

Query: 546 FYFMVSGLEAVNLGYFVLCSKWYKYKGS 570
           FYFM++G+ AVN  YF++ S+WY+YKGS
Sbjct: 574 FYFMIAGILAVNFAYFLVMSRWYRYKGS 594

BLAST of CmaCh10G001780 vs. TAIR 10
Match: AT1G18880.1 (Major facilitator superfamily protein )

HSP 1 Score: 649.0 bits (1673), Expect = 3.5e-186
Identity = 324/593 (54.64%), Postives = 433/593 (73.02%), Query Frame = 0

Query: 1   MERNNEDVHNLDEAKVNYKGVKALPFVVGNETFEKLGTTGTSSNLLVYLTDVFHMKTITA 60
           +E+  +++   DE+K+ Y+G K +PF++GNETFEKLG  G+SSNL++YLT VF+MK+ITA
Sbjct: 3   VEKTEKNIAEDDESKIIYRGWKVMPFIIGNETFEKLGIVGSSSNLVIYLTTVFNMKSITA 62

Query: 61  TTLMNIFHGSTNFSTLFGAFLCDTYFGRYKTLGYASIASLMGMVVLTLTATIERLHPPDC 120
             ++NI+ G++NF T+  AFLCD+YFGRYKTL +A IA  +G V + LTA I  LHP  C
Sbjct: 63  AKVVNIYGGTSNFGTIVAAFLCDSYFGRYKTLSFAMIACFLGSVAMDLTAVIHPLHPAQC 122

Query: 121 GKGSAAGGACLEPTPWQIAFLLFGLGLLIIGAGGIRPCNLAFGADQFNPNTASGKLGINS 180
            K    G  C  P+  QI FL   + LL+IGAGGIRPCNL FGADQF+P T  GK GI S
Sbjct: 123 AK--EIGSVCNGPSIGQIMFLAGAMVLLVIGAGGIRPCNLPFGADQFDPKTKEGKRGIES 182

Query: 181 FFNWYYFTFTFAMIISLTIIVYVQTEVSWAWGLAIPAFLMFLSCALFFMGSWIYVKLEPQ 240
           FFNWY+FTFTFA ++SLT+IVYVQ+ VSW+ GLAIPA LM L C +FF GS +YVK++  
Sbjct: 183 FFNWYFFTFTFAQMVSLTLIVYVQSNVSWSIGLAIPAILMLLGCIIFFAGSKLYVKVKAS 242

Query: 241 GSPFTSVMRVLVAALKKRRLPSPDQQWPSLFNHIPSNSINSKLPYTDQFSFLNKAAIITP 300
           GSP  S+ RV+V A+KKRRL         L+N+I S+  NSKL +T+QF FL+K+AI T 
Sbjct: 243 GSPIHSITRVIVVAIKKRRLKPVGPN--ELYNYIASDFKNSKLGHTEQFRFLDKSAIQTQ 302

Query: 301 EDKFKSDGSADDPWTLCSTQQVEEVKCLVRVIPIWGAAIMYHVATTQQQTYVVFQALQSD 360
           +DK   DGS  D W LCS QQVEEVKC++RV+P+W +A ++++A  QQ TY +FQ+LQSD
Sbjct: 303 DDKLNKDGSPVDAWKLCSMQQVEEVKCVIRVLPVWLSAALFYLAYIQQTTYTIFQSLQSD 362

Query: 361 RRLFFGNTHYFKIPAASYTIFTMIGLTIWIPFYDRIVVPSLRRITSREGGITLLQKMGIG 420
           RRL  G+   F+IPA SYT+F M+G+TI+IP YDR++VP LR+ T R+GGIT LQ++G G
Sbjct: 363 RRLGPGS---FQIPAGSYTVFLMLGMTIFIPIYDRVLVPFLRKYTGRDGGITQLQRVGAG 422

Query: 421 MVIAIVTMFISALVEQKRRNLALTQPLCEETGRRAAISSMSALWLVPQLTLIGLSEAFTV 480
           + + I +M +SA+VEQ RR +ALT+P      R+ AISSMS +WL+PQL L+G+++A   
Sbjct: 423 LFLCITSMMVSAIVEQYRRKVALTKPTLGLAPRKGAISSMSGMWLIPQLVLMGIADALAG 482

Query: 481 IAQVEFYYKEFPENMRSIGGSLSFVGLALSNYLSGFMVTVVHSLTT----GNWLPEDLNE 540
           + Q+EFYYK+FPENMRS  GSL + G+ L++YLS F+++ VH  T     G+WLPEDLN+
Sbjct: 483 VGQMEFYYKQFPENMRSFAGSLYYCGIGLASYLSTFLLSAVHDTTEGFSGGSWLPEDLNK 542

Query: 541 GRLDYFYFMVSGLEAVNLGYFVLCSKWYKYKGSGSHGVDEMDFGKTEFEKTVV 590
           GRL+YFYF+V+G+  +NL YF+L S WY+YK   +   D MD    EF+K  V
Sbjct: 543 GRLEYFYFLVAGMMTLNLAYFLLVSHWYRYKDVVAKDKD-MDKTSAEFDKVSV 587

BLAST of CmaCh10G001780 vs. TAIR 10
Match: AT1G69870.1 (nitrate transporter 1.7 )

HSP 1 Score: 484.6 bits (1246), Expect = 1.2e-136
Identity = 255/565 (45.13%), Postives = 370/565 (65.49%), Query Frame = 0

Query: 11  LDEAKVNYK--GVKALPFVVGNETFEKLGTTGTSSNLLVYLTDVFHMKTITATTLMNIFH 70
           LD  KV  K  G +A+ F++GNET E+LG+ G  +N +VYLT VFH++ + A  ++NI+ 
Sbjct: 43  LDAEKVEKKPGGWRAVSFILGNETLERLGSIGLLANFMVYLTKVFHLEQVDAANVINIWS 102

Query: 71  GSTNFSTLFGAFLCDTYFGRYKTLGYASIASLMGMVVLTLTATIERLHPPDCGKGSAAGG 130
           G TN + L GA++ DTY GR+KT+ +AS A+L+G++ +TLTA+  +LHP  C        
Sbjct: 103 GFTNLTPLVGAYISDTYVGRFKTIAFASFATLLGLITITLTASFPQLHPASCNSQDPL-- 162

Query: 131 ACLEPTPWQIAFLLFGLGLLIIGAGGIRPCNLAFGADQFNPNTASGKLGINSFFNWYYFT 190
           +C  P   QI  LL GL  L +G+GGIRPC++ FG DQF+  T  G  G+ SFFNWYY T
Sbjct: 163 SCGGPNKLQIGVLLLGLCFLSVGSGGIRPCSIPFGVDQFDQRTEEGVKGVASFFNWYYMT 222

Query: 191 FTFAMIISLTIIVYVQTEVSWAWGLAIPAFLMFLSCALFFMGSWIYVKLEPQGSPFTSVM 250
           FT  +II+ T++VY+Q +VSW  G +IP  LM L+  +FF G   YV ++P+GS F+ + 
Sbjct: 223 FTVVLIITQTVVVYIQDQVSWIIGFSIPTGLMALAVVMFFAGMKRYVYVKPEGSIFSGIA 282

Query: 251 RVLVAALKKR--RLPSPDQQWPSLFNHIPSNSINSKLPYTDQFSFLNKAAIITPEDKFKS 310
           +V+VAA KKR  +LP+ D    + ++    +S+ SKL  ++QF  L+KAA++  E     
Sbjct: 283 QVIVAARKKRKLKLPAEDDGTVTYYDPAIKSSVLSKLHRSNQFRCLDKAAVVI-EGDLTP 342

Query: 311 DGSADDPWTLCSTQQVEEVKCLVRVIPIWGAAIMYHVATTQQQTYVVFQALQSDRRLFFG 370
           +G   D W LCS Q+VEEVKCL+R++PIW A I+   A T Q T+ V QAL+ DR L   
Sbjct: 343 EGPPADKWRLCSVQEVEEVKCLIRIVPIWSAGIISLAAMTTQGTFTVSQALKMDRNL--- 402

Query: 371 NTHYFKIPAASYTIFTMIGLTIWIPFYDRIVVPSLRRITSREGGITLLQKMGIGMVIAIV 430
               F+IPA S ++ +++ + I++PFYDR+ VP +RRIT  + GITLLQ++G G+V AI 
Sbjct: 403 -GPKFEIPAGSLSVISLLTIGIFLPFYDRVFVPFMRRITGHKSGITLLQRIGTGIVFAIF 462

Query: 431 TMFISALVEQKRRNLALTQPLCEETGRRAAISSMSALWLVPQLTLIGLSEAFTVIAQVEF 490
           +M ++ +VE+ RR  ++        G    ++ MS  WL PQL L+GL EAF +I Q+EF
Sbjct: 463 SMIVAGIVERMRRIRSI------NAGDPTGMTPMSVFWLSPQLILMGLCEAFNIIGQIEF 522

Query: 491 YYKEFPENMRSIGGSLSFVGLALSNYLSGFMVTVVHSLTTG----NWLPEDLNEGRLDYF 550
           +  +FPE+MRSI  SL  +  A S+YLS F+VTVVH  + G    +WL ++LN G+LDYF
Sbjct: 523 FNSQFPEHMRSIANSLFSLSFAGSSYLSSFLVTVVHKFSGGHDRPDWLNKNLNAGKLDYF 582

Query: 551 YFMVSGLEAVNLGYFVLCSKWYKYK 568
           Y++++ L  VNL YF  C++ Y+YK
Sbjct: 583 YYLIAVLGVVNLVYFWYCARGYRYK 594

BLAST of CmaCh10G001780 vs. TAIR 10
Match: AT1G27080.1 (nitrate transporter 1.6 )

HSP 1 Score: 457.6 bits (1176), Expect = 1.5e-128
Identity = 247/554 (44.58%), Postives = 351/554 (63.36%), Query Frame = 0

Query: 20  GVKALPFVVGNETFEKLGTTGTSSNLLVYLTDVFHMKTITATTLMNIFHGSTNFSTLFGA 79
           G +A+ F++GNET EKLG+ G S+N ++YL +VFHM+ + A  +  ++ G TNF+ L GA
Sbjct: 17  GWRAITFILGNETLEKLGSIGVSANFMLYLRNVFHMEPVEAFNVYYLWMGLTNFAPLLGA 76

Query: 80  FLCDTYFGRYKTLGYASIASLMGMVVLTLTATIERLHPPDCGKGSAAGGACLEPTPWQIA 139
            + D Y GR+KT+ YAS+ S++G++ +TLTA + +LHPP C   +     C +P   Q+ 
Sbjct: 77  LISDAYIGRFKTIAYASLFSILGLMTVTLTACLPQLHPPPC--NNPHPDECDDPNKLQLG 136

Query: 140 FLLFGLGLLIIGAGGIRPCNLAFGADQFNPNTASGKLGINSFFNWYYFTFTFAMIISLTI 199
            L  GLG L IG+GGIRPC++ FG DQF+  T  G  G+ SFFNWYY T T  +I S T+
Sbjct: 137 ILFLGLGFLSIGSGGIRPCSIPFGVDQFDQRTEQGLKGVASFFNWYYLTLTMVLIFSHTV 196

Query: 200 IVYVQTEVSWAWGLAIPAFLMFLSCALFFMGSWIYVKLEPQGSPFTSVMRVLVAALKKR- 259
           +VY+QT VSW  G +IP  LM  +  LFF+G   YV ++P+GS F+ + RV+VAA KKR 
Sbjct: 197 VVYLQT-VSWVIGFSIPTSLMACAVVLFFVGMRFYVYVKPEGSVFSGIARVIVAARKKRD 256

Query: 260 -RLPSPDQQWPSLFNHIPSNSINSKLPYTDQFSFLNKAAIITPEDKFKSDGSADDPWTLC 319
            ++   D      +       + SKLP TDQF FL+KAA+I   D   S+G   + W LC
Sbjct: 257 LKISLVDDGTEEYYEPPVKPGVLSKLPLTDQFKFLDKAAVILDGD-LTSEGVPANKWRLC 316

Query: 320 STQQVEEVKCLVRVIPIWGAAIMYHVATTQQQTYVVFQALQSDRRLFFGNTHYFKIPAAS 379
           S Q+VEEVKCL+RV+P+W A I+  VA T Q T++VFQA + DR +      +F+IPAAS
Sbjct: 317 SIQEVEEVKCLIRVVPVWSAGIISIVAMTTQATFMVFQATKMDRHM----GPHFEIPAAS 376

Query: 380 YTIFTMIGLTIWIPFYDRIVVPSLRRITSREGGITLLQKMGIGMVIAIVTMFISALVEQK 439
            T+ + I + IW+P Y+ ++VP L R+  R+  +TLLQ+MGIG+V AI++MF +  VE  
Sbjct: 377 ITVISYITIGIWVPIYEHLLVPFLWRM--RKFRVTLLQRMGIGIVFAILSMFTAGFVEGV 436

Query: 440 RRNLALTQPLCEETGRRAAISSMSALWLVPQLTLIGLSEAFTVIAQVEFYYKEFPENMRS 499
           RR             R   ++ MS  WL   L L+GL E+F  I  +EF+  +FPE+MRS
Sbjct: 437 RRT------------RATEMTQMSVFWLALPLILMGLCESFNFIGLIEFFNSQFPEHMRS 496

Query: 500 IGGSLSFVGLALSNYLSGFMVTVVHSLT----TGNWLPEDLNEGRLDYFYFMVSGLEAVN 559
           I  SL  +  A +NYLS  +VT VH ++      +WL +DL+ G+LDYFY++++ L  VN
Sbjct: 497 IANSLFPLSFAAANYLSSLLVTTVHKVSGTKDHPDWLNKDLDRGKLDYFYYLIAVLGVVN 548

Query: 560 LGYFVLCSKWYKYK 568
           L YF  C+  Y+YK
Sbjct: 557 LVYFWYCAHRYQYK 548

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q944G55.3e-19557.64Protein NRT1/ PTR FAMILY 2.10 OS=Arabidopsis thaliana OX=3702 GN=NPF2.10 PE=1 SV... [more]
Q9LV103.0e-19057.92Protein NRT1/ PTR FAMILY 2.11 OS=Arabidopsis thaliana OX=3702 GN=NPF2.11 PE=1 SV... [more]
Q9M9V75.0e-18554.64Protein NRT1/ PTR FAMILY 2.9 OS=Arabidopsis thaliana OX=3702 GN=NPF2.9 PE=1 SV=1[more]
Q8RX771.6e-13545.13Protein NRT1/ PTR FAMILY 2.13 OS=Arabidopsis thaliana OX=3702 GN=NPF2.13 PE=1 SV... [more]
Q9LFX92.1e-12744.58Protein NRT1/ PTR FAMILY 2.12 OS=Arabidopsis thaliana OX=3702 GN=NPF2.12 PE=1 SV... [more]
Match NameE-valueIdentityDescription
A0A6J1JCU20.0e+00100.00protein NRT1/ PTR FAMILY 2.10-like OS=Cucurbita maxima OX=3661 GN=LOC111485624 P... [more]
A0A6J1E4K90.0e+0098.98protein NRT1/ PTR FAMILY 2.10-like OS=Cucurbita moschata OX=3662 GN=LOC111429868... [more]
A0A0A0LPB60.0e+0094.07Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G008540 PE=3 SV=1[more]
A0A5A7UPX70.0e+0093.73Protein NRT1/ PTR FAMILY 2.10-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C... [more]
A0A5D3BIU40.0e+0093.73Protein NRT1/ PTR FAMILY 2.10-like OS=Cucumis melo var. makuwa OX=1194695 GN=E56... [more]
Match NameE-valueIdentityDescription
XP_022988357.10.0e+00100.00protein NRT1/ PTR FAMILY 2.10-like [Cucurbita maxima][more]
XP_023515678.10.0e+0098.98protein NRT1/ PTR FAMILY 2.10-like isoform X1 [Cucurbita pepo subsp. pepo][more]
XP_022921698.10.0e+0098.98protein NRT1/ PTR FAMILY 2.10-like [Cucurbita moschata][more]
KAG7023179.10.0e+0098.98Protein NRT1/ PTR FAMILY 2.11, partial [Cucurbita argyrosperma subsp. argyrosper... [more]
KAG6589494.10.0e+0098.64Protein NRT1/ PTR FAMILY 2.11, partial [Cucurbita argyrosperma subsp. sororia][more]
Match NameE-valueIdentityDescription
AT3G47960.13.8e-19657.64Major facilitator superfamily protein [more]
AT5G62680.12.1e-19157.92Major facilitator superfamily protein [more]
AT1G18880.13.5e-18654.64Major facilitator superfamily protein [more]
AT1G69870.11.2e-13645.13nitrate transporter 1.7 [more]
AT1G27080.11.5e-12844.58nitrate transporter 1.6 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000109Proton-dependent oligopeptide transporter familyPFAMPF00854PTR2coord: 89..525
e-value: 4.2E-85
score: 286.0
IPR000109Proton-dependent oligopeptide transporter familyPANTHERPTHR11654OLIGOPEPTIDE TRANSPORTER-RELATEDcoord: 12..574
IPR036259MFS transporter superfamilyGENE3D1.20.1250.20MFS general substrate transporter like domainscoord: 1..571
e-value: 5.5E-168
score: 561.4
IPR036259MFS transporter superfamilySUPERFAMILY103473MFS general substrate transportercoord: 40..565
NoneNo IPR availablePANTHERPTHR11654:SF307PROTEIN NRT1/ PTR FAMILY 2.9coord: 12..574
NoneNo IPR availableCDDcd17416MFS_NPF1_2coord: 24..564
e-value: 0.0
score: 574.599

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh10G001780.1CmaCh10G001780.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005886 plasma membrane
cellular_component GO:0016020 membrane
molecular_function GO:0022857 transmembrane transporter activity