CmaCh10G000280 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh10G000280
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
DescriptionCCA-adding enzyme
LocationCma_Chr10: 101103 .. 105989 (-)
RNA-Seq ExpressionCmaCh10G000280
SyntenyCmaCh10G000280
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGCATATCGGGATTACTTGGCATGATGTCTTGCAGACCTTATCTCCCTCGACGGACACCACTCTTCCGTTTCCTCCGCAAGGTAATTCTGCTCCTATTGCCATTTCCTTTGCTTTCTCTTCCCTTCCCTCTTTCACCTAATTCATTCACCTTATTTACTTCAGCTTCGTCCCACTTCCATTGCAGTGGGGGAGGCAGCAGCTCCTCAAACAGATACCTTCTTCAACGGCCACACCGATTCTCCGCCTCCCCCAAGGAAATTTCAAGGGTTTTTCCTCCTACTTTCTAACTACTTTTAAGTTTCATAACTGGCTTCTTCTTGTTTCTGATAATATGAAACTAGGCCTCTGTTTCATTCTTAATTTTTTTTGACAAAAAAAAAAAAAACTGTTATTTTGCTGAAGAATTCCATGATAATGATTCTAAGCTGCGGAAGTGGAAGACGTTTTCTTCCAAGGAGCTTGGGATTGATATTTTCATGATTCCCAAACCTACCAGAAAAGTTCTGAACGGGCTCAAGAAAAAAGGTTACCCTACGTTGTCTCCTTCATTTACAAACGTCTCCTCTCACATCAAATAGTAAATAAACATTTAGTGTCATCTTCAAAATCTTAGCCTTTATCACCTTCTTCATGCACTTCTAGTATTTTGTAACTTTTGTAATTAATCCTGTGATTATTGTTTCACGTTCTAGGTTACGAAGTTTACCTTGTAGGAGGTTGTGTTCGCGATCTTATCTTGAACAGAACACCAAAGGATTTTGACATAATAACTTCAGCTGAGCTTAAGGAGGTTTGTCTCCAACCATCTCCTTCAAAATATTTGTATTACTTATATCGCTACCGAGGAAACTTTATTATCTATCATAAATACATAGAGTTGTGAGAAGAGACACATACACATGATAAATTAGTTTTCTCATATATATATTTTTTTTTTGATGATTAAAGAAGAATAGAAAGGAAACCCGTTAGATTAAAAAAGAATACTATTGGAAATCAGATTGCGAGTTGAAATTTCTACCATCTATCCTTACGAGACACTTCTCATTTCAATTCTTTATGCCGGAATGGTCAAGTGTGTTTTGTAGAATATGACGAGGCAGATTACTGGAAATTGGATGCTGAATATGTCCTTTATGTTTGGCAAAGATTATAATGTAAATTACATAAATCTCCGTGGAAAAAGGCCTCAATATCTTGGAACCTTTTTTGGGGGTCAAAAGCTCGATGAATATACATTGCAAGTTTCAGGTTCAACAGGGACTACTGACACTGTGAAGCCTCAGTTTTGCCTGTTTTCTCAATTTTAGTTATAAGTACTTTATATATATAATTTAAGGTTCGGCCCCTCATTTTTAACATATATTATATCGTACCATTTTAAGGTTTCAAGAACATTTTCATGGTGTGAAATAGTTGGGATGAAGTTTCCTATATGCCATGTGCATATTGATGGTAGCATTGTAGAGGTTCGTTTCTCTTCCTATCAATAAATGATTGGTACCTTTGACCATATGTAGTTGTTTCTGGTAGATATTACATCAATTTGATATTTTATGATTTCGTGTTTTAATAACGAGCTTTTACTTTCTTTAAAATATTAGGTGTCAAGTTTTAGTACATCCAGTAGGCCATTTGACAGACACTTGGACTCTGCTATTGAAAAACCAATGAACTGTGAAGAGGAAGATTATGTCCGATGGAAGAATTGCCTGCAACGTGACTTTACTATTAACGGGTGGCTTACGAGATTCTGCATCTTGAGAATCCATGTTCCTATCTCTATTATACAATTGTAATATTTTTTAAATAGTAGGTTTTCTGGTAAATGAAAATTTATAATTCTTGGGTCCCCCTCCAAACACATGCAGAAGAAAAAAAAAACGGCCGCTTTCACCCTTTGGTTCTCAACTAATAGTCAACTTCTTGCAGGCTGATGTATGATCCATACAACAGTGCAGTATACGACTACTTGGAAGGAATGGAGGATATAAAACAAGCTAAAGTATGCACACATTTCTCTCAAGTTATTAAATAAATACTGAAAGCCACACTAGATGATATTGTAAACTTCTTTTGTACGACCTTCTGGGAGCATAGATACGAACAGTGGTTCCTGCCTGCACTTCCTTTCAAGAGGATTGTGGTGAGTTTTCATTTGCAGAACTCCCTTATCGTATGTTGACTAGAGCATTGAGCTGAATGTATTGTTGAGAAACTTAAGTGAGCTTAAGCAGCGCATATGTACCCATAAAGCAATACTACTAACTCCATCTAGTTTGGCAGCACGAATCCTTCGAGCAATCAGAGTTGCAGCTCGTTTACGGTTCAATTTTGCGAAGGATACTGCTGGTTCTATTAAAAAATTATCCTGCCTGGTGTCTACCCTCAATAAGGTTTTTAGATTAACATGATATTTCCTTCCTAATGTTTCTTTGGATTTTAATATTCTTGAACGAGAATTCTAATTGATTTTCTTTCCTTAAAATATCAGGCAAGGCTTCAGATGGAAATGAACTATTTGTTGTCTTATGGTTCTGCTGAAGCTTCTTTGAGGTTGTTATGGAAATATGGACTTCTAGAAATACTTCTTCCAATTCAGGTGAATTGACGCTTGACTTTTTCCCCAGTTATGGGTTACAAATAAGTGAGACAGTTTGATATTGTAGGCAGCATATTTCATCCAATATGGTTTTCGGAGGTCCGACAAGGGATCAAATATGCTTTTGGTAATTGTCTCTTTCCTATTCTTACTATTACTGTCTTTTGGGTGCTTATCAGAATTTATTTTCTTTCAAGTTTTTCCTAATGTACGTGGAAGACCGAACTTATTTTTCACTTGGTACTGCATAAGACTTGGTAGTTTGCTAATCTCATGAAAAGTTGCATGTAATATATATATATATATTGTATATTATATAGTATAGTAGAAGATCAATATAATGTATGCAGTGTGGGTGGTTTTCCACAACTTTGTTATCTGAAATGTGGAAGGAAAAATTATTCATGGTTTGTTCTTCTAATGGTTAGTTGCACGTTGTGTGCTTTGAAAAAAAAAAATGTTTCTGGTAGATTTAAAGCTTTTGAATTACTTAGTTTCCTTTACTTTCTTTCGTTCACCGTCCCCAGTCCCCACTTCCCCAAGCCCTAAATAGCTCAAGAGTAAGTTATCTTGAATGCTTTCGGTATATTTTTCCTTGTCCTTTGAAATTATTCTGGAGTATACAAAGTATAAATCCCTTGGTGATGTATGCTGAATCATGCATGGAGTAAAGCTTTTGAAAAGTAAGAATAGGACATCGATTATGTCAAATTGATGATTTTCCTACCTGATTATGAATTGATGACACAATTGGTCCATGGCAATTACTTGTTATTAACTAACATTGTTAGTATGCAGTCCCTATTTTCCAGCATGGATAAACTTCTGGCACCTAATAGACCTTGTCACAGTAGTTTATGGTAAGATTGTTGTTGAGAACTAAAATCTACTGTTGTACTGTTGCTATCTATAATTATCGTCAGGGTTCAACATGGTGTCAGGATGTAGTGTAAACTACTCGTGTCTGATCTACAAAATTGGAATGTACCAATGCCAGTGAAGAGTGCTTAAAAAATAGGTTTAATATGTTATTGAAATGTAAAATTATATCTCTAGGTTACCCTAGAAGAGAGCTGAAATATTTTTTCCCCTTGAGTTATTGGTTGAATTTCAAAGTTGCGGTAAAGATGGTGGTGGAGTTTTGGAGGTGATTAGATTTAATACCTCGGTTTGGGCCATGGTGTCTAAGGCCTTCTGTAATGATTAGATTGGCCTTGTTCTCTTGGACGAGAGTCAGTTTTTTGGTTATTTGAGCTTCTTTTTTTTTTTTCATTTTGGTATGTGTTTTCGTAGTTGATAATATTTCTCAATGAAACCACGGTTTTTTATTTTTCTTAGAAAAAGATGACCGTTGTTTAGTTTTTGGCTTAAAGCTGAAACCTGCTGTTGGTCTGATCCTGTAAATTGTTCGGTGAACCATGATTCAAACTTGTTAATTAATATTTCGTTTATGAGCAGTGATAGTACTTGTTTTCCGAACTTTGTTCTCTCTTTTGCAGTGAAGCAGTCTCAAACACTGTTTTCTCTTACAGGGTTGCAGTATTAGCCTTCCATGTTGCCCTGTCTGACCAGCCTAGGAGTCCTTTAGTGGTTGCAGCATTTAGTCTTGCAGTCCACAATGGTGGCAATATGATGGAAGCAATTAGCATTGCTAAGAGCATCGGTAGAGCACATAATGTAAACTTTCACGAATTGTTAGAACCTGAGAATCTGGAGGTCCAAACTTTGATTGATGAGGTTATGGATCTTACTACGTCTATTAAGAACGCACTTCATAAGATGACCGACGAGCATTCTGTGTCTCTAGTCTTGGAAATGTATCCCCAAGCACCAGCCTCGTATCTAGTAATCTTTCTTCATTTTAATCTCTTGTACTTTTCAAAATAAAAAAGGTTGTTGCCAATTGTCTATACATTCAAAGTCAATTATAATTTTAGGTTCTAGAGAATATCAAGCTTATAGTAATCTACCCTACTCTTTCTCCTGTAATATAAATTATAGGATCCAAGTTTTTTTTTTTCCTACGACATATATTGCCTGTGGAATCATATGCTAAAAAGTTCACATATTGCAGGTTTTTATCCCATTGGTGGTGTACTTGAAGGTACGCAAAATTTTTGAGTGCGTTGTTGAGGGTGCAGAGGGAGTATTTGTTCCGAAGAGAGGAAAGGTTAATTACAAGTGTCTGGCTCTCGGGGACCTACCAGAACTTCGGCATGTTTTTGCAAGGATTGTATTTGATACTGTCTACCCTCTTGATCTCAATCATACTCGAGAATAA

mRNA sequence

ATGGGCATATCGGGATTACTTGGCATGATGTCTTGCAGACCTTATCTCCCTCGACGGACACCACTCTTCCGTTTCCTCCGCAAGTGGGGGAGGCAGCAGCTCCTCAAACAGATACCTTCTTCAACGGCCACACCGATTCTCCGCCTCCCCCAAGGAAATTTCAAGGAATTCCATGATAATGATTCTAAGCTGCGGAAGTGGAAGACGTTTTCTTCCAAGGAGCTTGGGATTGATATTTTCATGATTCCCAAACCTACCAGAAAAGTTCTGAACGGGCTCAAGAAAAAAGGTTACGAAGTTTACCTTGTAGGAGGTTGTGTTCGCGATCTTATCTTGAACAGAACACCAAAGGATTTTGACATAATAACTTCAGCTGAGCTTAAGGAGGTTTCAAGAACATTTTCATGGTGTGAAATAGTTGGGATGAAGTTTCCTATATGCCATGTGCATATTGATGGTAGCATTGTAGAGGTGTCAAGTTTTAGTACATCCAGTAGGCCATTTGACAGACACTTGGACTCTGCTATTGAAAAACCAATGAACTGTGAAGAGGAAGATTATGTCCGATGGAAGAATTGCCTGCAACGTGACTTTACTATTAACGGGCTGATGTATGATCCATACAACAGTGCAGTATACGACTACTTGGAAGGAATGGAGGATATAAAACAAGCTAAAATACGAACAGTGGTTCCTGCCTGCACTTCCTTTCAAGAGGATTGTGCACGAATCCTTCGAGCAATCAGAGTTGCAGCTCGTTTACGGTTCAATTTTGCGAAGGATACTGCTGGTTCTATTAAAAAATTATCCTGCCTGGTGTCTACCCTCAATAAGGCAAGGCTTCAGATGGAAATGAACTATTTGTTGTCTTATGGTTCTGCTGAAGCTTCTTTGAGGTTGTTATGGAAATATGGACTTCTAGAAATACTTCTTCCAATTCAGGCAGCATATTTCATCCAATATGGTTTTCGGAGGTCCGACAAGGGATCAAATATGCTTTTGTCCCTATTTTCCAGCATGGATAAACTTCTGGCACCTAATAGACCTTGTCACAGTAGTTTATGGGTTGCAGTATTAGCCTTCCATGTTGCCCTGTCTGACCAGCCTAGGAGTCCTTTAGTGGTTGCAGCATTTAGTCTTGCAGTCCACAATGGTGGCAATATGATGGAAGCAATTAGCATTGCTAAGAGCATCGGTAGAGCACATAATGTAAACTTTCACGAATTGTTAGAACCTGAGAATCTGGAGGTCCAAACTTTGATTGATGAGGTTATGGATCTTACTACGTCTATTAAGAACGCACTTCATAAGATGACCGACGAGCATTCTGTGTCTCTAGTCTTGGAAATGTATCCCCAAGCACCAGCCTCGTATCTAGTTTTTATCCCATTGGTGGTGTACTTGAAGGTACGCAAAATTTTTGAGTGCGTTGTTGAGGGTGCAGAGGGAGTATTTGTTCCGAAGAGAGGAAAGGTTAATTACAAGTGTCTGGCTCTCGGGGACCTACCAGAACTTCGGCATGTTTTTGCAAGGATTGTATTTGATACTGTCTACCCTCTTGATCTCAATCATACTCGAGAATAA

Coding sequence (CDS)

ATGGGCATATCGGGATTACTTGGCATGATGTCTTGCAGACCTTATCTCCCTCGACGGACACCACTCTTCCGTTTCCTCCGCAAGTGGGGGAGGCAGCAGCTCCTCAAACAGATACCTTCTTCAACGGCCACACCGATTCTCCGCCTCCCCCAAGGAAATTTCAAGGAATTCCATGATAATGATTCTAAGCTGCGGAAGTGGAAGACGTTTTCTTCCAAGGAGCTTGGGATTGATATTTTCATGATTCCCAAACCTACCAGAAAAGTTCTGAACGGGCTCAAGAAAAAAGGTTACGAAGTTTACCTTGTAGGAGGTTGTGTTCGCGATCTTATCTTGAACAGAACACCAAAGGATTTTGACATAATAACTTCAGCTGAGCTTAAGGAGGTTTCAAGAACATTTTCATGGTGTGAAATAGTTGGGATGAAGTTTCCTATATGCCATGTGCATATTGATGGTAGCATTGTAGAGGTGTCAAGTTTTAGTACATCCAGTAGGCCATTTGACAGACACTTGGACTCTGCTATTGAAAAACCAATGAACTGTGAAGAGGAAGATTATGTCCGATGGAAGAATTGCCTGCAACGTGACTTTACTATTAACGGGCTGATGTATGATCCATACAACAGTGCAGTATACGACTACTTGGAAGGAATGGAGGATATAAAACAAGCTAAAATACGAACAGTGGTTCCTGCCTGCACTTCCTTTCAAGAGGATTGTGCACGAATCCTTCGAGCAATCAGAGTTGCAGCTCGTTTACGGTTCAATTTTGCGAAGGATACTGCTGGTTCTATTAAAAAATTATCCTGCCTGGTGTCTACCCTCAATAAGGCAAGGCTTCAGATGGAAATGAACTATTTGTTGTCTTATGGTTCTGCTGAAGCTTCTTTGAGGTTGTTATGGAAATATGGACTTCTAGAAATACTTCTTCCAATTCAGGCAGCATATTTCATCCAATATGGTTTTCGGAGGTCCGACAAGGGATCAAATATGCTTTTGTCCCTATTTTCCAGCATGGATAAACTTCTGGCACCTAATAGACCTTGTCACAGTAGTTTATGGGTTGCAGTATTAGCCTTCCATGTTGCCCTGTCTGACCAGCCTAGGAGTCCTTTAGTGGTTGCAGCATTTAGTCTTGCAGTCCACAATGGTGGCAATATGATGGAAGCAATTAGCATTGCTAAGAGCATCGGTAGAGCACATAATGTAAACTTTCACGAATTGTTAGAACCTGAGAATCTGGAGGTCCAAACTTTGATTGATGAGGTTATGGATCTTACTACGTCTATTAAGAACGCACTTCATAAGATGACCGACGAGCATTCTGTGTCTCTAGTCTTGGAAATGTATCCCCAAGCACCAGCCTCGTATCTAGTTTTTATCCCATTGGTGGTGTACTTGAAGGTACGCAAAATTTTTGAGTGCGTTGTTGAGGGTGCAGAGGGAGTATTTGTTCCGAAGAGAGGAAAGGTTAATTACAAGTGTCTGGCTCTCGGGGACCTACCAGAACTTCGGCATGTTTTTGCAAGGATTGTATTTGATACTGTCTACCCTCTTGATCTCAATCATACTCGAGAATAA

Protein sequence

MGISGLLGMMSCRPYLPRRTPLFRFLRKWGRQQLLKQIPSSTATPILRLPQGNFKEFHDNDSKLRKWKTFSSKELGIDIFMIPKPTRKVLNGLKKKGYEVYLVGGCVRDLILNRTPKDFDIITSAELKEVSRTFSWCEIVGMKFPICHVHIDGSIVEVSSFSTSSRPFDRHLDSAIEKPMNCEEEDYVRWKNCLQRDFTINGLMYDPYNSAVYDYLEGMEDIKQAKIRTVVPACTSFQEDCARILRAIRVAARLRFNFAKDTAGSIKKLSCLVSTLNKARLQMEMNYLLSYGSAEASLRLLWKYGLLEILLPIQAAYFIQYGFRRSDKGSNMLLSLFSSMDKLLAPNRPCHSSLWVAVLAFHVALSDQPRSPLVVAAFSLAVHNGGNMMEAISIAKSIGRAHNVNFHELLEPENLEVQTLIDEVMDLTTSIKNALHKMTDEHSVSLVLEMYPQAPASYLVFIPLVVYLKVRKIFECVVEGAEGVFVPKRGKVNYKCLALGDLPELRHVFARIVFDTVYPLDLNHTRE
Homology
BLAST of CmaCh10G000280 vs. ExPASy Swiss-Prot
Match: P44439 (Poly(A) polymerase I OS=Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd) OX=71421 GN=pcnB PE=3 SV=1)

HSP 1 Score: 157.5 bits (397), Expect = 4.1e-37
Identity = 89/266 (33.46%), Postives = 145/266 (54.51%), Query Frame = 0

Query: 89  VLNGLKKKGYEVYLVGGCVRDLILNRTPKDFDIITSAELKEVSRTFS-WCEIVGMKFPIC 148
           V+  L+++G+E Y+VGGC+RDL+L + PKDFD+ T+A  +++   F   C +VG +F + 
Sbjct: 39  VVEKLQRQGFEAYIVGGCIRDLLLGKKPKDFDVATNARPEQIQNIFQRQCRLVGRRFRLA 98

Query: 149 HVHIDGSIVEVSSFSTSSRPFDRHLDSAIEKPMNCEEEDYVRWKN--------CLQRDFT 208
           H+     I+EV++F  +      H D+  E       E  +   N          +RDFT
Sbjct: 99  HIMFGRDIIEVATFRAN------HSDARNENQAKQSNEGMLLRDNVYGTIEQDAARRDFT 158

Query: 209 INGLMYDPYNSAVYDYLEGMEDIKQAKIRTVVPACTSFQEDCARILRAIRVAARLRFNFA 268
           +N L Y+P ++ + DY EG++D+K  K+R +    T +QED  R+LR+IR  A+L     
Sbjct: 159 VNALYYNPQDNTLRDYFEGIKDLKAGKLRLIGDPVTRYQEDPVRMLRSIRFMAKLDMFLE 218

Query: 269 KDTAGSIKKLSCLVSTLNKARLQMEMNYLLSYGSAEASLRLLWKYGLLEILLPIQAAYFI 328
           K +   I++L+ L+  +  ARL  E   LL  G    + RLL +YGL E L P  +AYF 
Sbjct: 219 KPSEQPIRELAPLLKNIPPARLFDESLKLLQAGQGVKTYRLLRQYGLFEQLFPALSAYFT 278

Query: 329 QYGFRRSDKGSNMLLSLFSSMDKLLA 346
           +   +       M+++  +S D+ +A
Sbjct: 279 E---KEDSFAERMIVTALTSTDERVA 295

BLAST of CmaCh10G000280 vs. ExPASy Swiss-Prot
Match: Q8ZRQ8 (Poly(A) polymerase I OS=Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) OX=99287 GN=pcnB PE=3 SV=2)

HSP 1 Score: 145.6 bits (366), Expect = 1.6e-33
Identity = 91/244 (37.30%), Postives = 133/244 (54.51%), Query Frame = 0

Query: 88  KVLNGLKKKGYEVYLVGGCVRDLILNRTPKDFDIITSAELKEVSRTFSWCEIVGMKFPIC 147
           KVL  L K GYE YLVGG VRDL+L + PKDFD+ T+A   +V + F  C +VG +F + 
Sbjct: 50  KVLYRLNKAGYEAYLVGGGVRDLLLGKKPKDFDVTTNATPDQVRKLFRNCRLVGRRFRLA 109

Query: 148 HVHIDGSIVEVSSF------STSSRPFDRHLDSAI---EKPMNCEEEDYVRWKNCLQRDF 207
           HV     I+EV++F      S S R   +   + +   +      EED  R      RDF
Sbjct: 110 HVMFGPEIIEVATFRGHHEGSESDRTTSQRGQNGMLLRDNIFGSIEEDAQR------RDF 169

Query: 208 TINGLMYDPYNSAVYDYLEGMEDIKQAKIRTVVPACTSFQEDCARILRAIRVAARLRFNF 267
           TIN L Y   +  V DY+ GM+D+++  IR +    T ++ED  R+LRA+R AA+L  + 
Sbjct: 170 TINSLYYSVADFTVRDYVGGMQDLQEGVIRLIGNPETRYREDPVRMLRAVRFAAKLNMHI 229

Query: 268 AKDTAGSIKKLSCLVSTLNKARLQMEMNYLLSYGSAEASLRLLWKYGLLEILLPIQAAYF 323
           + +TA  I +L+ L++ +  ARL  E   LL  G+   + + L +Y L + L P    YF
Sbjct: 230 SPETAEPIPRLATLLNDIPPARLFEESLKLLQAGNGYETYQQLREYHLFQPLFPTITRYF 287

BLAST of CmaCh10G000280 vs. ExPASy Swiss-Prot
Match: Q8Z9C3 (Poly(A) polymerase I OS=Salmonella typhi OX=90370 GN=pcnB PE=3 SV=2)

HSP 1 Score: 144.8 bits (364), Expect = 2.7e-33
Identity = 91/244 (37.30%), Postives = 132/244 (54.10%), Query Frame = 0

Query: 88  KVLNGLKKKGYEVYLVGGCVRDLILNRTPKDFDIITSAELKEVSRTFSWCEIVGMKFPIC 147
           KVL  L K GYE YLVGG VRDL+L + PKDFD+ T+A   +V + F  C +VG +F + 
Sbjct: 50  KVLYRLNKAGYEAYLVGGGVRDLLLGKKPKDFDVTTNATPDQVRKLFRNCRLVGRRFRLA 109

Query: 148 HVHIDGSIVEVSSF------STSSRPFDRHLDSAI---EKPMNCEEEDYVRWKNCLQRDF 207
           HV     I+EV++F      S S R   +   + +   +      EED  R      RDF
Sbjct: 110 HVMFGPEIIEVATFRGHNEGSESDRTTSQRGQNGMLLRDNIFGSIEEDAQR------RDF 169

Query: 208 TINGLMYDPYNSAVYDYLEGMEDIKQAKIRTVVPACTSFQEDCARILRAIRVAARLRFNF 267
           TIN L Y   +  V DY+ GM+D+++  IR +    T ++ED  R+LRA+R AA+L    
Sbjct: 170 TINSLYYSVADFTVRDYVGGMQDLQEGVIRLIGNPETRYREDPVRMLRAVRFAAKLNMRI 229

Query: 268 AKDTAGSIKKLSCLVSTLNKARLQMEMNYLLSYGSAEASLRLLWKYGLLEILLPIQAAYF 323
           + +TA  I +L+ L++ +  ARL  E   LL  G+   + + L +Y L + L P    YF
Sbjct: 230 SPETAEPIPRLATLLNDIPPARLFEESLKLLQAGNGFETYQQLREYHLFQPLFPTITRYF 287

BLAST of CmaCh10G000280 vs. ExPASy Swiss-Prot
Match: P0ABF3 (Poly(A) polymerase I OS=Escherichia coli O157:H7 OX=83334 GN=pcnB PE=3 SV=2)

HSP 1 Score: 144.1 bits (362), Expect = 4.7e-33
Identity = 90/244 (36.89%), Postives = 132/244 (54.10%), Query Frame = 0

Query: 88  KVLNGLKKKGYEVYLVGGCVRDLILNRTPKDFDIITSAELKEVSRTFSWCEIVGMKFPIC 147
           KV+  L K GYE +LVGG VRDL+L + PKDFD+ T+A  ++V + F  C +VG +F + 
Sbjct: 50  KVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKLFRNCRLVGRRFRLA 109

Query: 148 HVHIDGSIVEVSSF------STSSRPFDRHLDSAI---EKPMNCEEEDYVRWKNCLQRDF 207
           HV     I+EV++F      + S R   +   + +   +      EED  R      RDF
Sbjct: 110 HVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGSIEEDAQR------RDF 169

Query: 208 TINGLMYDPYNSAVYDYLEGMEDIKQAKIRTVVPACTSFQEDCARILRAIRVAARLRFNF 267
           TIN L Y   +  V DY+ GM+D+K   IR +    T ++ED  R+LRA+R AA+L    
Sbjct: 170 TINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLRAVRFAAKLGMRI 229

Query: 268 AKDTAGSIKKLSCLVSTLNKARLQMEMNYLLSYGSAEASLRLLWKYGLLEILLPIQAAYF 323
           + +TA  I +L+ L++ +  ARL  E   LL  G    + +LL +Y L + L P    YF
Sbjct: 230 SPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYETYKLLCEYHLFQPLFPTITRYF 287

BLAST of CmaCh10G000280 vs. ExPASy Swiss-Prot
Match: P0ABF2 (Poly(A) polymerase I OS=Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC) OX=199310 GN=pcnB PE=3 SV=2)

HSP 1 Score: 144.1 bits (362), Expect = 4.7e-33
Identity = 90/244 (36.89%), Postives = 132/244 (54.10%), Query Frame = 0

Query: 88  KVLNGLKKKGYEVYLVGGCVRDLILNRTPKDFDIITSAELKEVSRTFSWCEIVGMKFPIC 147
           KV+  L K GYE +LVGG VRDL+L + PKDFD+ T+A  ++V + F  C +VG +F + 
Sbjct: 50  KVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKLFRNCRLVGRRFRLA 109

Query: 148 HVHIDGSIVEVSSF------STSSRPFDRHLDSAI---EKPMNCEEEDYVRWKNCLQRDF 207
           HV     I+EV++F      + S R   +   + +   +      EED  R      RDF
Sbjct: 110 HVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGSIEEDAQR------RDF 169

Query: 208 TINGLMYDPYNSAVYDYLEGMEDIKQAKIRTVVPACTSFQEDCARILRAIRVAARLRFNF 267
           TIN L Y   +  V DY+ GM+D+K   IR +    T ++ED  R+LRA+R AA+L    
Sbjct: 170 TINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLRAVRFAAKLGMRI 229

Query: 268 AKDTAGSIKKLSCLVSTLNKARLQMEMNYLLSYGSAEASLRLLWKYGLLEILLPIQAAYF 323
           + +TA  I +L+ L++ +  ARL  E   LL  G    + +LL +Y L + L P    YF
Sbjct: 230 SPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYETYKLLCEYHLFQPLFPTITRYF 287

BLAST of CmaCh10G000280 vs. ExPASy TrEMBL
Match: A0A6J1JFZ7 (uncharacterized protein LOC111484780 isoform X4 OS=Cucurbita maxima OX=3661 GN=LOC111484780 PE=3 SV=1)

HSP 1 Score: 1060.4 bits (2741), Expect = 2.4e-306
Identity = 527/527 (100.00%), Postives = 527/527 (100.00%), Query Frame = 0

Query: 1   MGISGLLGMMSCRPYLPRRTPLFRFLRKWGRQQLLKQIPSSTATPILRLPQGNFKEFHDN 60
           MGISGLLGMMSCRPYLPRRTPLFRFLRKWGRQQLLKQIPSSTATPILRLPQGNFKEFHDN
Sbjct: 1   MGISGLLGMMSCRPYLPRRTPLFRFLRKWGRQQLLKQIPSSTATPILRLPQGNFKEFHDN 60

Query: 61  DSKLRKWKTFSSKELGIDIFMIPKPTRKVLNGLKKKGYEVYLVGGCVRDLILNRTPKDFD 120
           DSKLRKWKTFSSKELGIDIFMIPKPTRKVLNGLKKKGYEVYLVGGCVRDLILNRTPKDFD
Sbjct: 61  DSKLRKWKTFSSKELGIDIFMIPKPTRKVLNGLKKKGYEVYLVGGCVRDLILNRTPKDFD 120

Query: 121 IITSAELKEVSRTFSWCEIVGMKFPICHVHIDGSIVEVSSFSTSSRPFDRHLDSAIEKPM 180
           IITSAELKEVSRTFSWCEIVGMKFPICHVHIDGSIVEVSSFSTSSRPFDRHLDSAIEKPM
Sbjct: 121 IITSAELKEVSRTFSWCEIVGMKFPICHVHIDGSIVEVSSFSTSSRPFDRHLDSAIEKPM 180

Query: 181 NCEEEDYVRWKNCLQRDFTINGLMYDPYNSAVYDYLEGMEDIKQAKIRTVVPACTSFQED 240
           NCEEEDYVRWKNCLQRDFTINGLMYDPYNSAVYDYLEGMEDIKQAKIRTVVPACTSFQED
Sbjct: 181 NCEEEDYVRWKNCLQRDFTINGLMYDPYNSAVYDYLEGMEDIKQAKIRTVVPACTSFQED 240

Query: 241 CARILRAIRVAARLRFNFAKDTAGSIKKLSCLVSTLNKARLQMEMNYLLSYGSAEASLRL 300
           CARILRAIRVAARLRFNFAKDTAGSIKKLSCLVSTLNKARLQMEMNYLLSYGSAEASLRL
Sbjct: 241 CARILRAIRVAARLRFNFAKDTAGSIKKLSCLVSTLNKARLQMEMNYLLSYGSAEASLRL 300

Query: 301 LWKYGLLEILLPIQAAYFIQYGFRRSDKGSNMLLSLFSSMDKLLAPNRPCHSSLWVAVLA 360
           LWKYGLLEILLPIQAAYFIQYGFRRSDKGSNMLLSLFSSMDKLLAPNRPCHSSLWVAVLA
Sbjct: 301 LWKYGLLEILLPIQAAYFIQYGFRRSDKGSNMLLSLFSSMDKLLAPNRPCHSSLWVAVLA 360

Query: 361 FHVALSDQPRSPLVVAAFSLAVHNGGNMMEAISIAKSIGRAHNVNFHELLEPENLEVQTL 420
           FHVALSDQPRSPLVVAAFSLAVHNGGNMMEAISIAKSIGRAHNVNFHELLEPENLEVQTL
Sbjct: 361 FHVALSDQPRSPLVVAAFSLAVHNGGNMMEAISIAKSIGRAHNVNFHELLEPENLEVQTL 420

Query: 421 IDEVMDLTTSIKNALHKMTDEHSVSLVLEMYPQAPASYLVFIPLVVYLKVRKIFECVVEG 480
           IDEVMDLTTSIKNALHKMTDEHSVSLVLEMYPQAPASYLVFIPLVVYLKVRKIFECVVEG
Sbjct: 421 IDEVMDLTTSIKNALHKMTDEHSVSLVLEMYPQAPASYLVFIPLVVYLKVRKIFECVVEG 480

Query: 481 AEGVFVPKRGKVNYKCLALGDLPELRHVFARIVFDTVYPLDLNHTRE 528
           AEGVFVPKRGKVNYKCLALGDLPELRHVFARIVFDTVYPLDLNHTRE
Sbjct: 481 AEGVFVPKRGKVNYKCLALGDLPELRHVFARIVFDTVYPLDLNHTRE 527

BLAST of CmaCh10G000280 vs. ExPASy TrEMBL
Match: A0A6J1JIL3 (uncharacterized protein LOC111484780 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111484780 PE=3 SV=1)

HSP 1 Score: 1048.9 bits (2711), Expect = 7.1e-303
Identity = 527/546 (96.52%), Postives = 527/546 (96.52%), Query Frame = 0

Query: 1   MGISGLLGMMSCRPYLPRRTPLFRFLRKWGRQQLLKQIPSSTATPILRLPQGNFKEFHDN 60
           MGISGLLGMMSCRPYLPRRTPLFRFLRKWGRQQLLKQIPSSTATPILRLPQGNFKEFHDN
Sbjct: 1   MGISGLLGMMSCRPYLPRRTPLFRFLRKWGRQQLLKQIPSSTATPILRLPQGNFKEFHDN 60

Query: 61  DSKLRKWKTFSSKELGIDIFMIPKPTRKVLNGLKKKGYEVYLVGGCVRDLILNRTPKDFD 120
           DSKLRKWKTFSSKELGIDIFMIPKPTRKVLNGLKKKGYEVYLVGGCVRDLILNRTPKDFD
Sbjct: 61  DSKLRKWKTFSSKELGIDIFMIPKPTRKVLNGLKKKGYEVYLVGGCVRDLILNRTPKDFD 120

Query: 121 IITSAELKEVSRTFSWCEIVGMKFPICHVHIDGSIVEVSSFSTSSRPFDRHLDSAIEKPM 180
           IITSAELKEVSRTFSWCEIVGMKFPICHVHIDGSIVEVSSFSTSSRPFDRHLDSAIEKPM
Sbjct: 121 IITSAELKEVSRTFSWCEIVGMKFPICHVHIDGSIVEVSSFSTSSRPFDRHLDSAIEKPM 180

Query: 181 NCEEEDYVRWKNCLQRDFTING-------------------LMYDPYNSAVYDYLEGMED 240
           NCEEEDYVRWKNCLQRDFTING                   LMYDPYNSAVYDYLEGMED
Sbjct: 181 NCEEEDYVRWKNCLQRDFTINGWLTRFCILRIHVPISIIQLLMYDPYNSAVYDYLEGMED 240

Query: 241 IKQAKIRTVVPACTSFQEDCARILRAIRVAARLRFNFAKDTAGSIKKLSCLVSTLNKARL 300
           IKQAKIRTVVPACTSFQEDCARILRAIRVAARLRFNFAKDTAGSIKKLSCLVSTLNKARL
Sbjct: 241 IKQAKIRTVVPACTSFQEDCARILRAIRVAARLRFNFAKDTAGSIKKLSCLVSTLNKARL 300

Query: 301 QMEMNYLLSYGSAEASLRLLWKYGLLEILLPIQAAYFIQYGFRRSDKGSNMLLSLFSSMD 360
           QMEMNYLLSYGSAEASLRLLWKYGLLEILLPIQAAYFIQYGFRRSDKGSNMLLSLFSSMD
Sbjct: 301 QMEMNYLLSYGSAEASLRLLWKYGLLEILLPIQAAYFIQYGFRRSDKGSNMLLSLFSSMD 360

Query: 361 KLLAPNRPCHSSLWVAVLAFHVALSDQPRSPLVVAAFSLAVHNGGNMMEAISIAKSIGRA 420
           KLLAPNRPCHSSLWVAVLAFHVALSDQPRSPLVVAAFSLAVHNGGNMMEAISIAKSIGRA
Sbjct: 361 KLLAPNRPCHSSLWVAVLAFHVALSDQPRSPLVVAAFSLAVHNGGNMMEAISIAKSIGRA 420

Query: 421 HNVNFHELLEPENLEVQTLIDEVMDLTTSIKNALHKMTDEHSVSLVLEMYPQAPASYLVF 480
           HNVNFHELLEPENLEVQTLIDEVMDLTTSIKNALHKMTDEHSVSLVLEMYPQAPASYLVF
Sbjct: 421 HNVNFHELLEPENLEVQTLIDEVMDLTTSIKNALHKMTDEHSVSLVLEMYPQAPASYLVF 480

Query: 481 IPLVVYLKVRKIFECVVEGAEGVFVPKRGKVNYKCLALGDLPELRHVFARIVFDTVYPLD 528
           IPLVVYLKVRKIFECVVEGAEGVFVPKRGKVNYKCLALGDLPELRHVFARIVFDTVYPLD
Sbjct: 481 IPLVVYLKVRKIFECVVEGAEGVFVPKRGKVNYKCLALGDLPELRHVFARIVFDTVYPLD 540

BLAST of CmaCh10G000280 vs. ExPASy TrEMBL
Match: A0A6J1JI15 (uncharacterized protein LOC111484780 isoform X3 OS=Cucurbita maxima OX=3661 GN=LOC111484780 PE=3 SV=1)

HSP 1 Score: 1047.0 bits (2706), Expect = 2.7e-302
Identity = 526/538 (97.77%), Postives = 526/538 (97.77%), Query Frame = 0

Query: 1   MGISGLLGMMSCRPYLPRRTPLFRFLRKWGRQQLLKQIPSSTATPILRLPQGNFKEFHDN 60
           MGISGLLGMMSCRPYLPRRTPLFRFLRKWGRQQLLKQIPSSTATPILRLPQGNFKEFHDN
Sbjct: 1   MGISGLLGMMSCRPYLPRRTPLFRFLRKWGRQQLLKQIPSSTATPILRLPQGNFKEFHDN 60

Query: 61  DSKLRKWKTFSSKELGIDIFMIPKPTRKVLNGLKKKGYEVYLVGGCVRDLILNRTPKDFD 120
           DSKLRKWKTFSSKELGIDIFMIPKPTRKVLNGLKKKGYEVYLVGGCVRDLILNRTPKDFD
Sbjct: 61  DSKLRKWKTFSSKELGIDIFMIPKPTRKVLNGLKKKGYEVYLVGGCVRDLILNRTPKDFD 120

Query: 121 IITSAELKEVSRTFSWCEIVGMKFPICHVHIDGSIVEVSSFSTSSRPFDRHLDSAIEKPM 180
           IITSAELKEVSRTFSWCEIVGMKFPICHVHIDGSIVEVSSFSTSSRPFDRHLDSAIEKPM
Sbjct: 121 IITSAELKEVSRTFSWCEIVGMKFPICHVHIDGSIVEVSSFSTSSRPFDRHLDSAIEKPM 180

Query: 181 NCEEEDYVRWKNCLQRDFTINGLMYDPYNSAVYDYLEGMEDIKQAKIRTVVPACTSFQED 240
           NCEEEDYVRWKNCLQRDFTINGLMYDPYNSAVYDYLEGMEDIKQAKIRTVVPACTSFQED
Sbjct: 181 NCEEEDYVRWKNCLQRDFTINGLMYDPYNSAVYDYLEGMEDIKQAKIRTVVPACTSFQED 240

Query: 241 CARILRAIRVAARLRFNFAKDTAGSIKKLSCLVSTLNKARLQMEMNYLLSYGSAEASLRL 300
           CARILRAIRVAARLRFNFAKDTAGSIKKLSCLVSTLNKARLQMEMNYLLSYGSAEASLRL
Sbjct: 241 CARILRAIRVAARLRFNFAKDTAGSIKKLSCLVSTLNKARLQMEMNYLLSYGSAEASLRL 300

Query: 301 LWKYGLLEILLPIQAAYFIQYGFRRSDKGSNMLLSLFSSMDKLLAPNRPCHSSLW----- 360
           LWKYGLLEILLPIQAAYFIQYGFRRSDKGSNMLLSLFSSMDKLLAPNRPCHSSL      
Sbjct: 301 LWKYGLLEILLPIQAAYFIQYGFRRSDKGSNMLLSLFSSMDKLLAPNRPCHSSLCEAVSN 360

Query: 361 ------VAVLAFHVALSDQPRSPLVVAAFSLAVHNGGNMMEAISIAKSIGRAHNVNFHEL 420
                 VAVLAFHVALSDQPRSPLVVAAFSLAVHNGGNMMEAISIAKSIGRAHNVNFHEL
Sbjct: 361 TVFSYRVAVLAFHVALSDQPRSPLVVAAFSLAVHNGGNMMEAISIAKSIGRAHNVNFHEL 420

Query: 421 LEPENLEVQTLIDEVMDLTTSIKNALHKMTDEHSVSLVLEMYPQAPASYLVFIPLVVYLK 480
           LEPENLEVQTLIDEVMDLTTSIKNALHKMTDEHSVSLVLEMYPQAPASYLVFIPLVVYLK
Sbjct: 421 LEPENLEVQTLIDEVMDLTTSIKNALHKMTDEHSVSLVLEMYPQAPASYLVFIPLVVYLK 480

Query: 481 VRKIFECVVEGAEGVFVPKRGKVNYKCLALGDLPELRHVFARIVFDTVYPLDLNHTRE 528
           VRKIFECVVEGAEGVFVPKRGKVNYKCLALGDLPELRHVFARIVFDTVYPLDLNHTRE
Sbjct: 481 VRKIFECVVEGAEGVFVPKRGKVNYKCLALGDLPELRHVFARIVFDTVYPLDLNHTRE 538

BLAST of CmaCh10G000280 vs. ExPASy TrEMBL
Match: A0A6J1J9J1 (uncharacterized protein LOC111484780 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111484780 PE=3 SV=1)

HSP 1 Score: 1035.4 bits (2676), Expect = 8.2e-299
Identity = 526/557 (94.43%), Postives = 526/557 (94.43%), Query Frame = 0

Query: 1   MGISGLLGMMSCRPYLPRRTPLFRFLRKWGRQQLLKQIPSSTATPILRLPQGNFKEFHDN 60
           MGISGLLGMMSCRPYLPRRTPLFRFLRKWGRQQLLKQIPSSTATPILRLPQGNFKEFHDN
Sbjct: 1   MGISGLLGMMSCRPYLPRRTPLFRFLRKWGRQQLLKQIPSSTATPILRLPQGNFKEFHDN 60

Query: 61  DSKLRKWKTFSSKELGIDIFMIPKPTRKVLNGLKKKGYEVYLVGGCVRDLILNRTPKDFD 120
           DSKLRKWKTFSSKELGIDIFMIPKPTRKVLNGLKKKGYEVYLVGGCVRDLILNRTPKDFD
Sbjct: 61  DSKLRKWKTFSSKELGIDIFMIPKPTRKVLNGLKKKGYEVYLVGGCVRDLILNRTPKDFD 120

Query: 121 IITSAELKEVSRTFSWCEIVGMKFPICHVHIDGSIVEVSSFSTSSRPFDRHLDSAIEKPM 180
           IITSAELKEVSRTFSWCEIVGMKFPICHVHIDGSIVEVSSFSTSSRPFDRHLDSAIEKPM
Sbjct: 121 IITSAELKEVSRTFSWCEIVGMKFPICHVHIDGSIVEVSSFSTSSRPFDRHLDSAIEKPM 180

Query: 181 NCEEEDYVRWKNCLQRDFTING-------------------LMYDPYNSAVYDYLEGMED 240
           NCEEEDYVRWKNCLQRDFTING                   LMYDPYNSAVYDYLEGMED
Sbjct: 181 NCEEEDYVRWKNCLQRDFTINGWLTRFCILRIHVPISIIQLLMYDPYNSAVYDYLEGMED 240

Query: 241 IKQAKIRTVVPACTSFQEDCARILRAIRVAARLRFNFAKDTAGSIKKLSCLVSTLNKARL 300
           IKQAKIRTVVPACTSFQEDCARILRAIRVAARLRFNFAKDTAGSIKKLSCLVSTLNKARL
Sbjct: 241 IKQAKIRTVVPACTSFQEDCARILRAIRVAARLRFNFAKDTAGSIKKLSCLVSTLNKARL 300

Query: 301 QMEMNYLLSYGSAEASLRLLWKYGLLEILLPIQAAYFIQYGFRRSDKGSNMLLSLFSSMD 360
           QMEMNYLLSYGSAEASLRLLWKYGLLEILLPIQAAYFIQYGFRRSDKGSNMLLSLFSSMD
Sbjct: 301 QMEMNYLLSYGSAEASLRLLWKYGLLEILLPIQAAYFIQYGFRRSDKGSNMLLSLFSSMD 360

Query: 361 KLLAPNRPCHSSLW-----------VAVLAFHVALSDQPRSPLVVAAFSLAVHNGGNMME 420
           KLLAPNRPCHSSL            VAVLAFHVALSDQPRSPLVVAAFSLAVHNGGNMME
Sbjct: 361 KLLAPNRPCHSSLCEAVSNTVFSYRVAVLAFHVALSDQPRSPLVVAAFSLAVHNGGNMME 420

Query: 421 AISIAKSIGRAHNVNFHELLEPENLEVQTLIDEVMDLTTSIKNALHKMTDEHSVSLVLEM 480
           AISIAKSIGRAHNVNFHELLEPENLEVQTLIDEVMDLTTSIKNALHKMTDEHSVSLVLEM
Sbjct: 421 AISIAKSIGRAHNVNFHELLEPENLEVQTLIDEVMDLTTSIKNALHKMTDEHSVSLVLEM 480

Query: 481 YPQAPASYLVFIPLVVYLKVRKIFECVVEGAEGVFVPKRGKVNYKCLALGDLPELRHVFA 528
           YPQAPASYLVFIPLVVYLKVRKIFECVVEGAEGVFVPKRGKVNYKCLALGDLPELRHVFA
Sbjct: 481 YPQAPASYLVFIPLVVYLKVRKIFECVVEGAEGVFVPKRGKVNYKCLALGDLPELRHVFA 540

BLAST of CmaCh10G000280 vs. ExPASy TrEMBL
Match: A0A6J1E1V4 (uncharacterized protein LOC111429822 isoform X4 OS=Cucurbita moschata OX=3662 GN=LOC111429822 PE=3 SV=1)

HSP 1 Score: 1028.5 bits (2658), Expect = 1.0e-296
Identity = 514/527 (97.53%), Postives = 520/527 (98.67%), Query Frame = 0

Query: 1   MGISGLLGMMSCRPYLPRRTPLFRFLRKWGRQQLLKQIPSSTATPILRLPQGNFKEFHDN 60
           MG+SGLLG+MSCRPYLPRRTPLFRFLRKWGRQQLLKQIPSSTATPILRLP+GNFKEF DN
Sbjct: 1   MGMSGLLGIMSCRPYLPRRTPLFRFLRKWGRQQLLKQIPSSTATPILRLPRGNFKEF-DN 60

Query: 61  DSKLRKWKTFSSKELGIDIFMIPKPTRKVLNGLKKKGYEVYLVGGCVRDLILNRTPKDFD 120
           DSKLRKWKTFSSKELGIDIFMIPKPTRKVLNGLKKKGYEVYLVGGCVRDLILNRTPKDFD
Sbjct: 61  DSKLRKWKTFSSKELGIDIFMIPKPTRKVLNGLKKKGYEVYLVGGCVRDLILNRTPKDFD 120

Query: 121 IITSAELKEVSRTFSWCEIVGMKFPICHVHIDGSIVEVSSFSTSSRPFDRHLDSAIEKPM 180
           IITSAELKEVSRTFSWCEIVG KFPICHVHIDGSIVEVSSFSTSSRPFDRHL+SAIEKPM
Sbjct: 121 IITSAELKEVSRTFSWCEIVGRKFPICHVHIDGSIVEVSSFSTSSRPFDRHLNSAIEKPM 180

Query: 181 NCEEEDYVRWKNCLQRDFTINGLMYDPYNSAVYDYLEGMEDIKQAKIRTVVPACTSFQED 240
           NCEEEDYVRWKNC+QRDFTINGLMYDPYNSAVYDYLEGMEDIKQAKIRTVVPACTSFQED
Sbjct: 181 NCEEEDYVRWKNCMQRDFTINGLMYDPYNSAVYDYLEGMEDIKQAKIRTVVPACTSFQED 240

Query: 241 CARILRAIRVAARLRFNFAKDTAGSIKKLSCLVSTLNKARLQMEMNYLLSYGSAEASLRL 300
           CARILRAIRVAARLRFNFAKDTAGSIKKLSCLVSTLNKARLQMEMNYLLSYGSAEASLRL
Sbjct: 241 CARILRAIRVAARLRFNFAKDTAGSIKKLSCLVSTLNKARLQMEMNYLLSYGSAEASLRL 300

Query: 301 LWKYGLLEILLPIQAAYFIQYGFRRSDKGSNMLLSLFSSMDKLLAPNRPCHSSLWVAVLA 360
           LWKYGLLEILLPIQAAYFIQYGFRRSDKGSNMLLSLFSSMDKLLAPNRPCHSSLWVAVLA
Sbjct: 301 LWKYGLLEILLPIQAAYFIQYGFRRSDKGSNMLLSLFSSMDKLLAPNRPCHSSLWVAVLA 360

Query: 361 FHVALSDQPRSPLVVAAFSLAVHNGGNMMEAISIAKSIGRAHNVNFHELLEPENLEVQTL 420
           FHVALSDQPRSPLVVAAFSLAVHNGGNMMEAISIAKSI RAHNVNFHELLEPENLEVQTL
Sbjct: 361 FHVALSDQPRSPLVVAAFSLAVHNGGNMMEAISIAKSISRAHNVNFHELLEPENLEVQTL 420

Query: 421 IDEVMDLTTSIKNALHKMTDEHSVSLVLEMYPQAPASYLVFIPLVVYLKVRKIFECVVEG 480
           IDEVMDLTTSIKNALHKMTDEHSVSLVLEMYPQAPAS LVFIPLVVYLKVRKIFEC VEG
Sbjct: 421 IDEVMDLTTSIKNALHKMTDEHSVSLVLEMYPQAPASDLVFIPLVVYLKVRKIFECFVEG 480

Query: 481 AEGVFVPKRGKVNYKCLALGDLPELRHVFARIVFDTVYPLDLNHTRE 528
           AE VFVPKRGKVNY+CLALGDLPELRH FARIVFDTVYPLDLNHTRE
Sbjct: 481 AERVFVPKRGKVNYECLALGDLPELRHFFARIVFDTVYPLDLNHTRE 526

BLAST of CmaCh10G000280 vs. NCBI nr
Match: XP_022987140.1 (uncharacterized protein LOC111484780 isoform X4 [Cucurbita maxima])

HSP 1 Score: 1060.4 bits (2741), Expect = 4.9e-306
Identity = 527/527 (100.00%), Postives = 527/527 (100.00%), Query Frame = 0

Query: 1   MGISGLLGMMSCRPYLPRRTPLFRFLRKWGRQQLLKQIPSSTATPILRLPQGNFKEFHDN 60
           MGISGLLGMMSCRPYLPRRTPLFRFLRKWGRQQLLKQIPSSTATPILRLPQGNFKEFHDN
Sbjct: 1   MGISGLLGMMSCRPYLPRRTPLFRFLRKWGRQQLLKQIPSSTATPILRLPQGNFKEFHDN 60

Query: 61  DSKLRKWKTFSSKELGIDIFMIPKPTRKVLNGLKKKGYEVYLVGGCVRDLILNRTPKDFD 120
           DSKLRKWKTFSSKELGIDIFMIPKPTRKVLNGLKKKGYEVYLVGGCVRDLILNRTPKDFD
Sbjct: 61  DSKLRKWKTFSSKELGIDIFMIPKPTRKVLNGLKKKGYEVYLVGGCVRDLILNRTPKDFD 120

Query: 121 IITSAELKEVSRTFSWCEIVGMKFPICHVHIDGSIVEVSSFSTSSRPFDRHLDSAIEKPM 180
           IITSAELKEVSRTFSWCEIVGMKFPICHVHIDGSIVEVSSFSTSSRPFDRHLDSAIEKPM
Sbjct: 121 IITSAELKEVSRTFSWCEIVGMKFPICHVHIDGSIVEVSSFSTSSRPFDRHLDSAIEKPM 180

Query: 181 NCEEEDYVRWKNCLQRDFTINGLMYDPYNSAVYDYLEGMEDIKQAKIRTVVPACTSFQED 240
           NCEEEDYVRWKNCLQRDFTINGLMYDPYNSAVYDYLEGMEDIKQAKIRTVVPACTSFQED
Sbjct: 181 NCEEEDYVRWKNCLQRDFTINGLMYDPYNSAVYDYLEGMEDIKQAKIRTVVPACTSFQED 240

Query: 241 CARILRAIRVAARLRFNFAKDTAGSIKKLSCLVSTLNKARLQMEMNYLLSYGSAEASLRL 300
           CARILRAIRVAARLRFNFAKDTAGSIKKLSCLVSTLNKARLQMEMNYLLSYGSAEASLRL
Sbjct: 241 CARILRAIRVAARLRFNFAKDTAGSIKKLSCLVSTLNKARLQMEMNYLLSYGSAEASLRL 300

Query: 301 LWKYGLLEILLPIQAAYFIQYGFRRSDKGSNMLLSLFSSMDKLLAPNRPCHSSLWVAVLA 360
           LWKYGLLEILLPIQAAYFIQYGFRRSDKGSNMLLSLFSSMDKLLAPNRPCHSSLWVAVLA
Sbjct: 301 LWKYGLLEILLPIQAAYFIQYGFRRSDKGSNMLLSLFSSMDKLLAPNRPCHSSLWVAVLA 360

Query: 361 FHVALSDQPRSPLVVAAFSLAVHNGGNMMEAISIAKSIGRAHNVNFHELLEPENLEVQTL 420
           FHVALSDQPRSPLVVAAFSLAVHNGGNMMEAISIAKSIGRAHNVNFHELLEPENLEVQTL
Sbjct: 361 FHVALSDQPRSPLVVAAFSLAVHNGGNMMEAISIAKSIGRAHNVNFHELLEPENLEVQTL 420

Query: 421 IDEVMDLTTSIKNALHKMTDEHSVSLVLEMYPQAPASYLVFIPLVVYLKVRKIFECVVEG 480
           IDEVMDLTTSIKNALHKMTDEHSVSLVLEMYPQAPASYLVFIPLVVYLKVRKIFECVVEG
Sbjct: 421 IDEVMDLTTSIKNALHKMTDEHSVSLVLEMYPQAPASYLVFIPLVVYLKVRKIFECVVEG 480

Query: 481 AEGVFVPKRGKVNYKCLALGDLPELRHVFARIVFDTVYPLDLNHTRE 528
           AEGVFVPKRGKVNYKCLALGDLPELRHVFARIVFDTVYPLDLNHTRE
Sbjct: 481 AEGVFVPKRGKVNYKCLALGDLPELRHVFARIVFDTVYPLDLNHTRE 527

BLAST of CmaCh10G000280 vs. NCBI nr
Match: XP_022987138.1 (uncharacterized protein LOC111484780 isoform X2 [Cucurbita maxima])

HSP 1 Score: 1048.9 bits (2711), Expect = 1.5e-302
Identity = 527/546 (96.52%), Postives = 527/546 (96.52%), Query Frame = 0

Query: 1   MGISGLLGMMSCRPYLPRRTPLFRFLRKWGRQQLLKQIPSSTATPILRLPQGNFKEFHDN 60
           MGISGLLGMMSCRPYLPRRTPLFRFLRKWGRQQLLKQIPSSTATPILRLPQGNFKEFHDN
Sbjct: 1   MGISGLLGMMSCRPYLPRRTPLFRFLRKWGRQQLLKQIPSSTATPILRLPQGNFKEFHDN 60

Query: 61  DSKLRKWKTFSSKELGIDIFMIPKPTRKVLNGLKKKGYEVYLVGGCVRDLILNRTPKDFD 120
           DSKLRKWKTFSSKELGIDIFMIPKPTRKVLNGLKKKGYEVYLVGGCVRDLILNRTPKDFD
Sbjct: 61  DSKLRKWKTFSSKELGIDIFMIPKPTRKVLNGLKKKGYEVYLVGGCVRDLILNRTPKDFD 120

Query: 121 IITSAELKEVSRTFSWCEIVGMKFPICHVHIDGSIVEVSSFSTSSRPFDRHLDSAIEKPM 180
           IITSAELKEVSRTFSWCEIVGMKFPICHVHIDGSIVEVSSFSTSSRPFDRHLDSAIEKPM
Sbjct: 121 IITSAELKEVSRTFSWCEIVGMKFPICHVHIDGSIVEVSSFSTSSRPFDRHLDSAIEKPM 180

Query: 181 NCEEEDYVRWKNCLQRDFTING-------------------LMYDPYNSAVYDYLEGMED 240
           NCEEEDYVRWKNCLQRDFTING                   LMYDPYNSAVYDYLEGMED
Sbjct: 181 NCEEEDYVRWKNCLQRDFTINGWLTRFCILRIHVPISIIQLLMYDPYNSAVYDYLEGMED 240

Query: 241 IKQAKIRTVVPACTSFQEDCARILRAIRVAARLRFNFAKDTAGSIKKLSCLVSTLNKARL 300
           IKQAKIRTVVPACTSFQEDCARILRAIRVAARLRFNFAKDTAGSIKKLSCLVSTLNKARL
Sbjct: 241 IKQAKIRTVVPACTSFQEDCARILRAIRVAARLRFNFAKDTAGSIKKLSCLVSTLNKARL 300

Query: 301 QMEMNYLLSYGSAEASLRLLWKYGLLEILLPIQAAYFIQYGFRRSDKGSNMLLSLFSSMD 360
           QMEMNYLLSYGSAEASLRLLWKYGLLEILLPIQAAYFIQYGFRRSDKGSNMLLSLFSSMD
Sbjct: 301 QMEMNYLLSYGSAEASLRLLWKYGLLEILLPIQAAYFIQYGFRRSDKGSNMLLSLFSSMD 360

Query: 361 KLLAPNRPCHSSLWVAVLAFHVALSDQPRSPLVVAAFSLAVHNGGNMMEAISIAKSIGRA 420
           KLLAPNRPCHSSLWVAVLAFHVALSDQPRSPLVVAAFSLAVHNGGNMMEAISIAKSIGRA
Sbjct: 361 KLLAPNRPCHSSLWVAVLAFHVALSDQPRSPLVVAAFSLAVHNGGNMMEAISIAKSIGRA 420

Query: 421 HNVNFHELLEPENLEVQTLIDEVMDLTTSIKNALHKMTDEHSVSLVLEMYPQAPASYLVF 480
           HNVNFHELLEPENLEVQTLIDEVMDLTTSIKNALHKMTDEHSVSLVLEMYPQAPASYLVF
Sbjct: 421 HNVNFHELLEPENLEVQTLIDEVMDLTTSIKNALHKMTDEHSVSLVLEMYPQAPASYLVF 480

Query: 481 IPLVVYLKVRKIFECVVEGAEGVFVPKRGKVNYKCLALGDLPELRHVFARIVFDTVYPLD 528
           IPLVVYLKVRKIFECVVEGAEGVFVPKRGKVNYKCLALGDLPELRHVFARIVFDTVYPLD
Sbjct: 481 IPLVVYLKVRKIFECVVEGAEGVFVPKRGKVNYKCLALGDLPELRHVFARIVFDTVYPLD 540

BLAST of CmaCh10G000280 vs. NCBI nr
Match: XP_022987139.1 (uncharacterized protein LOC111484780 isoform X3 [Cucurbita maxima])

HSP 1 Score: 1047.0 bits (2706), Expect = 5.6e-302
Identity = 526/538 (97.77%), Postives = 526/538 (97.77%), Query Frame = 0

Query: 1   MGISGLLGMMSCRPYLPRRTPLFRFLRKWGRQQLLKQIPSSTATPILRLPQGNFKEFHDN 60
           MGISGLLGMMSCRPYLPRRTPLFRFLRKWGRQQLLKQIPSSTATPILRLPQGNFKEFHDN
Sbjct: 1   MGISGLLGMMSCRPYLPRRTPLFRFLRKWGRQQLLKQIPSSTATPILRLPQGNFKEFHDN 60

Query: 61  DSKLRKWKTFSSKELGIDIFMIPKPTRKVLNGLKKKGYEVYLVGGCVRDLILNRTPKDFD 120
           DSKLRKWKTFSSKELGIDIFMIPKPTRKVLNGLKKKGYEVYLVGGCVRDLILNRTPKDFD
Sbjct: 61  DSKLRKWKTFSSKELGIDIFMIPKPTRKVLNGLKKKGYEVYLVGGCVRDLILNRTPKDFD 120

Query: 121 IITSAELKEVSRTFSWCEIVGMKFPICHVHIDGSIVEVSSFSTSSRPFDRHLDSAIEKPM 180
           IITSAELKEVSRTFSWCEIVGMKFPICHVHIDGSIVEVSSFSTSSRPFDRHLDSAIEKPM
Sbjct: 121 IITSAELKEVSRTFSWCEIVGMKFPICHVHIDGSIVEVSSFSTSSRPFDRHLDSAIEKPM 180

Query: 181 NCEEEDYVRWKNCLQRDFTINGLMYDPYNSAVYDYLEGMEDIKQAKIRTVVPACTSFQED 240
           NCEEEDYVRWKNCLQRDFTINGLMYDPYNSAVYDYLEGMEDIKQAKIRTVVPACTSFQED
Sbjct: 181 NCEEEDYVRWKNCLQRDFTINGLMYDPYNSAVYDYLEGMEDIKQAKIRTVVPACTSFQED 240

Query: 241 CARILRAIRVAARLRFNFAKDTAGSIKKLSCLVSTLNKARLQMEMNYLLSYGSAEASLRL 300
           CARILRAIRVAARLRFNFAKDTAGSIKKLSCLVSTLNKARLQMEMNYLLSYGSAEASLRL
Sbjct: 241 CARILRAIRVAARLRFNFAKDTAGSIKKLSCLVSTLNKARLQMEMNYLLSYGSAEASLRL 300

Query: 301 LWKYGLLEILLPIQAAYFIQYGFRRSDKGSNMLLSLFSSMDKLLAPNRPCHSSLW----- 360
           LWKYGLLEILLPIQAAYFIQYGFRRSDKGSNMLLSLFSSMDKLLAPNRPCHSSL      
Sbjct: 301 LWKYGLLEILLPIQAAYFIQYGFRRSDKGSNMLLSLFSSMDKLLAPNRPCHSSLCEAVSN 360

Query: 361 ------VAVLAFHVALSDQPRSPLVVAAFSLAVHNGGNMMEAISIAKSIGRAHNVNFHEL 420
                 VAVLAFHVALSDQPRSPLVVAAFSLAVHNGGNMMEAISIAKSIGRAHNVNFHEL
Sbjct: 361 TVFSYRVAVLAFHVALSDQPRSPLVVAAFSLAVHNGGNMMEAISIAKSIGRAHNVNFHEL 420

Query: 421 LEPENLEVQTLIDEVMDLTTSIKNALHKMTDEHSVSLVLEMYPQAPASYLVFIPLVVYLK 480
           LEPENLEVQTLIDEVMDLTTSIKNALHKMTDEHSVSLVLEMYPQAPASYLVFIPLVVYLK
Sbjct: 421 LEPENLEVQTLIDEVMDLTTSIKNALHKMTDEHSVSLVLEMYPQAPASYLVFIPLVVYLK 480

Query: 481 VRKIFECVVEGAEGVFVPKRGKVNYKCLALGDLPELRHVFARIVFDTVYPLDLNHTRE 528
           VRKIFECVVEGAEGVFVPKRGKVNYKCLALGDLPELRHVFARIVFDTVYPLDLNHTRE
Sbjct: 481 VRKIFECVVEGAEGVFVPKRGKVNYKCLALGDLPELRHVFARIVFDTVYPLDLNHTRE 538

BLAST of CmaCh10G000280 vs. NCBI nr
Match: XP_023516570.1 (uncharacterized protein LOC111780413 isoform X4 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1037.7 bits (2682), Expect = 3.4e-299
Identity = 517/527 (98.10%), Postives = 521/527 (98.86%), Query Frame = 0

Query: 1   MGISGLLGMMSCRPYLPRRTPLFRFLRKWGRQQLLKQIPSSTATPILRLPQGNFKEFHDN 60
           MGISGLLGMMSCRPYLPRRTPLFRFLRKWGRQQLLKQIPSSTATPILRLPQGNFKEF DN
Sbjct: 1   MGISGLLGMMSCRPYLPRRTPLFRFLRKWGRQQLLKQIPSSTATPILRLPQGNFKEFDDN 60

Query: 61  DSKLRKWKTFSSKELGIDIFMIPKPTRKVLNGLKKKGYEVYLVGGCVRDLILNRTPKDFD 120
           DSKLRKWKTFSSKELGIDIFMIPKPTR+VLNGLKKKGYEVYLVGGCVRDLILNRTPKDFD
Sbjct: 61  DSKLRKWKTFSSKELGIDIFMIPKPTRQVLNGLKKKGYEVYLVGGCVRDLILNRTPKDFD 120

Query: 121 IITSAELKEVSRTFSWCEIVGMKFPICHVHIDGSIVEVSSFSTSSRPFDRHLDSAIEKPM 180
           IITSAELKEVSRTFSWCEIVG KFPICHVHIDGSIVEVSSFSTSSRPFDRHL+SAIEKPM
Sbjct: 121 IITSAELKEVSRTFSWCEIVGRKFPICHVHIDGSIVEVSSFSTSSRPFDRHLNSAIEKPM 180

Query: 181 NCEEEDYVRWKNCLQRDFTINGLMYDPYNSAVYDYLEGMEDIKQAKIRTVVPACTSFQED 240
           NCEEEDYVRWKNCLQRDFTINGLMYDPYNSAVYDYLEGMEDIKQAKIRTVVPACTSFQED
Sbjct: 181 NCEEEDYVRWKNCLQRDFTINGLMYDPYNSAVYDYLEGMEDIKQAKIRTVVPACTSFQED 240

Query: 241 CARILRAIRVAARLRFNFAKDTAGSIKKLSCLVSTLNKARLQMEMNYLLSYGSAEASLRL 300
           CARILRAIRVAARLRFNFAKDTAGSIKKLSCLVSTLNKARLQMEMNYLLSYGSAEASLRL
Sbjct: 241 CARILRAIRVAARLRFNFAKDTAGSIKKLSCLVSTLNKARLQMEMNYLLSYGSAEASLRL 300

Query: 301 LWKYGLLEILLPIQAAYFIQYGFRRSDKGSNMLLSLFSSMDKLLAPNRPCHSSLWVAVLA 360
           LWKYGLLEILLPIQAAYFIQYGFRRSDKGSNMLLSLFSSMDKLLAPNRPCHSS+WVAVLA
Sbjct: 301 LWKYGLLEILLPIQAAYFIQYGFRRSDKGSNMLLSLFSSMDKLLAPNRPCHSSIWVAVLA 360

Query: 361 FHVALSDQPRSPLVVAAFSLAVHNGGNMMEAISIAKSIGRAHNVNFHELLEPENLEVQTL 420
           FHVALSDQPRSPLVVAAFSLAVHNGGNMMEAISIAKSI RAHNVNFHELLEPENLEVQTL
Sbjct: 361 FHVALSDQPRSPLVVAAFSLAVHNGGNMMEAISIAKSISRAHNVNFHELLEPENLEVQTL 420

Query: 421 IDEVMDLTTSIKNALHKMTDEHSVSLVLEMYPQAPASYLVFIPLVVYLKVRKIFECVVEG 480
           IDEVMDLTTSIKNALHKMTDEHSVSLVLEMYPQAPAS LVFIPLVVYLKVRKIFECVVEG
Sbjct: 421 IDEVMDLTTSIKNALHKMTDEHSVSLVLEMYPQAPASDLVFIPLVVYLKVRKIFECVVEG 480

Query: 481 AEGVFVPKRGKVNYKCLALGDLPELRHVFARIVFDTVYPLDLNHTRE 528
           AE VFVPKRGKVNY+CLALGDLPELRH FARIVFDTVYPLDLNHTRE
Sbjct: 481 AERVFVPKRGKVNYECLALGDLPELRHFFARIVFDTVYPLDLNHTRE 527

BLAST of CmaCh10G000280 vs. NCBI nr
Match: XP_022987137.1 (uncharacterized protein LOC111484780 isoform X1 [Cucurbita maxima])

HSP 1 Score: 1035.4 bits (2676), Expect = 1.7e-298
Identity = 526/557 (94.43%), Postives = 526/557 (94.43%), Query Frame = 0

Query: 1   MGISGLLGMMSCRPYLPRRTPLFRFLRKWGRQQLLKQIPSSTATPILRLPQGNFKEFHDN 60
           MGISGLLGMMSCRPYLPRRTPLFRFLRKWGRQQLLKQIPSSTATPILRLPQGNFKEFHDN
Sbjct: 1   MGISGLLGMMSCRPYLPRRTPLFRFLRKWGRQQLLKQIPSSTATPILRLPQGNFKEFHDN 60

Query: 61  DSKLRKWKTFSSKELGIDIFMIPKPTRKVLNGLKKKGYEVYLVGGCVRDLILNRTPKDFD 120
           DSKLRKWKTFSSKELGIDIFMIPKPTRKVLNGLKKKGYEVYLVGGCVRDLILNRTPKDFD
Sbjct: 61  DSKLRKWKTFSSKELGIDIFMIPKPTRKVLNGLKKKGYEVYLVGGCVRDLILNRTPKDFD 120

Query: 121 IITSAELKEVSRTFSWCEIVGMKFPICHVHIDGSIVEVSSFSTSSRPFDRHLDSAIEKPM 180
           IITSAELKEVSRTFSWCEIVGMKFPICHVHIDGSIVEVSSFSTSSRPFDRHLDSAIEKPM
Sbjct: 121 IITSAELKEVSRTFSWCEIVGMKFPICHVHIDGSIVEVSSFSTSSRPFDRHLDSAIEKPM 180

Query: 181 NCEEEDYVRWKNCLQRDFTING-------------------LMYDPYNSAVYDYLEGMED 240
           NCEEEDYVRWKNCLQRDFTING                   LMYDPYNSAVYDYLEGMED
Sbjct: 181 NCEEEDYVRWKNCLQRDFTINGWLTRFCILRIHVPISIIQLLMYDPYNSAVYDYLEGMED 240

Query: 241 IKQAKIRTVVPACTSFQEDCARILRAIRVAARLRFNFAKDTAGSIKKLSCLVSTLNKARL 300
           IKQAKIRTVVPACTSFQEDCARILRAIRVAARLRFNFAKDTAGSIKKLSCLVSTLNKARL
Sbjct: 241 IKQAKIRTVVPACTSFQEDCARILRAIRVAARLRFNFAKDTAGSIKKLSCLVSTLNKARL 300

Query: 301 QMEMNYLLSYGSAEASLRLLWKYGLLEILLPIQAAYFIQYGFRRSDKGSNMLLSLFSSMD 360
           QMEMNYLLSYGSAEASLRLLWKYGLLEILLPIQAAYFIQYGFRRSDKGSNMLLSLFSSMD
Sbjct: 301 QMEMNYLLSYGSAEASLRLLWKYGLLEILLPIQAAYFIQYGFRRSDKGSNMLLSLFSSMD 360

Query: 361 KLLAPNRPCHSSLW-----------VAVLAFHVALSDQPRSPLVVAAFSLAVHNGGNMME 420
           KLLAPNRPCHSSL            VAVLAFHVALSDQPRSPLVVAAFSLAVHNGGNMME
Sbjct: 361 KLLAPNRPCHSSLCEAVSNTVFSYRVAVLAFHVALSDQPRSPLVVAAFSLAVHNGGNMME 420

Query: 421 AISIAKSIGRAHNVNFHELLEPENLEVQTLIDEVMDLTTSIKNALHKMTDEHSVSLVLEM 480
           AISIAKSIGRAHNVNFHELLEPENLEVQTLIDEVMDLTTSIKNALHKMTDEHSVSLVLEM
Sbjct: 421 AISIAKSIGRAHNVNFHELLEPENLEVQTLIDEVMDLTTSIKNALHKMTDEHSVSLVLEM 480

Query: 481 YPQAPASYLVFIPLVVYLKVRKIFECVVEGAEGVFVPKRGKVNYKCLALGDLPELRHVFA 528
           YPQAPASYLVFIPLVVYLKVRKIFECVVEGAEGVFVPKRGKVNYKCLALGDLPELRHVFA
Sbjct: 481 YPQAPASYLVFIPLVVYLKVRKIFECVVEGAEGVFVPKRGKVNYKCLALGDLPELRHVFA 540

BLAST of CmaCh10G000280 vs. TAIR 10
Match: AT5G23690.1 (Polynucleotide adenylyltransferase family protein )

HSP 1 Score: 580.5 bits (1495), Expect = 1.4e-165
Identity = 294/463 (63.50%), Postives = 368/463 (79.48%), Query Frame = 0

Query: 65  RKWKTFSSKELGIDIFMIPKPTRKVLNGLKKKGYEVYLVGGCVRDLILNRTPKDFDIITS 124
           R+WK  +SK+LG+   MI K TRKVLNGLK KG++VYLVGGCVRDLIL RTPKDFDI+TS
Sbjct: 62  REWKQLNSKDLGLSSSMIAKSTRKVLNGLKSKGHDVYLVGGCVRDLILKRTPKDFDILTS 121

Query: 125 AELKEVSRTFSWCEIVGMKFPICHVHIDGSIVEVSSFSTSSRPFDRHLDSAIEKPMNCE- 184
           AEL+EV RTF  CEIVG +FPICHVHI   ++EVSSFSTS++   R+  +  ++    + 
Sbjct: 122 AELREVVRTFPRCEIVGRRFPICHVHIGDDLIEVSSFSTSAQNSSRNTRTECKESSGSDG 181

Query: 185 EEDYVRWKNCLQRDFTINGLMYDPYNSAVYDYLEGMEDIKQAKIRTVVPACTSFQEDCAR 244
           +ED +R  NCLQRDFTINGLM+DPY   VYDYL GMEDI++AK+RTV+ A TSF +DCAR
Sbjct: 182 DEDCIRLNNCLQRDFTINGLMFDPYAKVVYDYLGGMEDIRKAKVRTVIHAGTSFHQDCAR 241

Query: 245 ILRAIRVAARLRFNFAKDTAGSIKKLSCLVSTLNKARLQMEMNYLLSYGSAEASLRLLWK 304
           ILRAIR+AARL F  +K+TA  IK LS LV  L+K R+ MEMNY+L+YGSAEASLRLLWK
Sbjct: 242 ILRAIRIAARLGFRMSKETAHFIKNLSLLVQRLDKGRILMEMNYMLAYGSAEASLRLLWK 301

Query: 305 YGLLEILLPIQAAYFIQYGFRRSDKGSNMLLSLFSSMDKLLAPNRPCHSSLWVAVLAFHV 364
           +G+LEILLPIQAAY  + GFRR DK +NMLLSLF+++DKLLAP+RPCHSSLW+A+LAFH 
Sbjct: 302 FGILEILLPIQAAYLARSGFRRRDKRTNMLLSLFANLDKLLAPDRPCHSSLWIAILAFHK 361

Query: 365 ALSDQPRSPLVVAAFSLAVHNGGNMMEAISIAKSIGRAHNVNFHELLEP-ENLEVQTLID 424
           AL+D+PRSP+VVAAFSLAVHN G+++EA+ I K I R H+ +F EL+EP ENL+ QTL+D
Sbjct: 362 ALADKPRSPIVVAAFSLAVHNCGDILEAVEITKKITRPHDKSFFELVEPEENLDFQTLLD 421

Query: 425 EVMDLTTSIKNALHKMTDEHSVSLVLEMYPQAPASYLVFIPLVVYLKVRKIFECVVEGAE 484
           EVMDL  SI++AL++MTD + +S  +  YPQAP S LVFIPL +YL+  +IF+CV     
Sbjct: 422 EVMDLDASIEDALNQMTDAYFISKAMSAYPQAPYSDLVFIPLQLYLRAGRIFDCVKNEET 481

Query: 485 GV-FVPKRG-KVNYKCLALGDLPELRHVFARIVFDTVYPLDLN 524
            + F  K+G K+ Y  L  G  PE+RHVFAR+VFDTV+PL+L+
Sbjct: 482 RIGFEAKQGSKIEYGSLNSGYFPEIRHVFARVVFDTVFPLNLS 524

BLAST of CmaCh10G000280 vs. TAIR 10
Match: AT3G48830.1 (polynucleotide adenylyltransferase family protein / RNA recognition motif (RRM)-containing protein )

HSP 1 Score: 543.5 bits (1399), Expect = 1.9e-154
Identity = 279/483 (57.76%), Postives = 354/483 (73.29%), Query Frame = 0

Query: 7   LGMMSCRPYLPRRTPLFRFLRKWGRQQLLKQIPSSTATPILRLPQGNFKEFHDNDSKLRK 66
           +G  +CR + P   PLF    K      L  + ++  T   +    +  +  +  SK  +
Sbjct: 6   VGFFACRSHFP-VLPLFYCFSK----VQLNTVAAAMETVDDKDSDHHHDKGSNKTSKAPE 65

Query: 67  WKTFSSKELGIDIFMIPKPTRKVLNGLKKKGYEVYLVGGCVRDLILNRTPKDFDIITSAE 126
           WK  +SK+LGI   MI KPTR VLNGLK KGY+VYLVGGCVRDLIL RTPKDFDI+TSAE
Sbjct: 66  WKKLNSKDLGITTSMISKPTRIVLNGLKSKGYDVYLVGGCVRDLILKRTPKDFDILTSAE 125

Query: 127 LKEVSRTFSWCEIVGMKFPICHVHIDGSIVEVSSFSTSSRPFDRHLDSAIEKPMNCEEED 186
           L+EV R+FS CEI+G KFPICHVHI   ++EVSSFSTS++   R+  +   K     +ED
Sbjct: 126 LREVVRSFSRCEIIGKKFPICHVHIGNDMIEVSSFSTSAQNSLRNTRTGSGKSNGSYDED 185

Query: 187 YVRWKNCLQRDFTINGLMYDPYNSAVYDYLEGMEDIKQAKIRTVVPACTSFQEDCARILR 246
            +R+ NCLQRDFTINGLM+DPY   +YDYL G+EDIK+AK+RTV  A TSFQED ARILR
Sbjct: 186 SIRFNNCLQRDFTINGLMFDPYAKVIYDYLGGIEDIKKAKVRTVFHAGTSFQEDSARILR 245

Query: 247 AIRVAARLRFNFAKDTAGSIKKLSCLVSTLNKARLQMEMNYLLSYGSAEASLRLLWKYGL 306
             R+AARL F  +K+TA  +K LS LV  L++ R+ +EMNY+L+YGSAEASLRLLWK+G+
Sbjct: 246 GTRIAARLGFTISKETAHFLKNLSFLVQRLHRGRILLEMNYMLAYGSAEASLRLLWKFGI 305

Query: 307 LEILLPIQAAYFIQYGFRRSDKGSNMLLSLFSSMDKLLAPNRPCHSSLWVAVLAFHVALS 366
           LEILLPIQAAY ++ GF+R DK SN+LLSLF ++DKLLAP++PCHSSLW+ +LA H AL+
Sbjct: 306 LEILLPIQAAYLVRTGFKRRDKRSNLLLSLFGNLDKLLAPDKPCHSSLWLTILALHKALA 365

Query: 367 DQPRSPLVVAAFSLAVHNGGNMMEAISIAKSIGRAHNVNFHELLEPENLEVQTLIDEVMD 426
           DQPR P VVAAFSLAVHNGG+++EA+   + + + HN +F ELLEPE ++ QTL+DEVMD
Sbjct: 366 DQPRYPSVVAAFSLAVHNGGDVLEAVKNTRKVTKPHNRSFFELLEPEEMDSQTLLDEVMD 425

Query: 427 LTTSIKNALHKMTDEHSVSLVLEMYPQAPASYLVFIPLVVYLKVRKIFECVVEGAEGVFV 486
             +SIK AL +MTD   +S  +  YPQAP S +VFIPL +YL  R+IFECV E  +  FV
Sbjct: 426 FDSSIKEALGQMTDGRFISKAMAAYPQAPYSDMVFIPLQLYLDARRIFECVKENGQKGFV 483

Query: 487 PKR 490
           PK+
Sbjct: 486 PKQ 483

BLAST of CmaCh10G000280 vs. TAIR 10
Match: AT1G28090.1 (Polynucleotide adenylyltransferase family protein )

HSP 1 Score: 495.4 bits (1274), Expect = 5.8e-140
Identity = 252/467 (53.96%), Postives = 334/467 (71.52%), Query Frame = 0

Query: 60  NDSKLRKWKTFSSKELGIDIFMIPKPTRKVLNGLKKKGYEVYLVGGCVRDLILNRTPKDF 119
           N  K   WK   + E GI   MIP  TR VLN LKKKG++VYLVGGCVRDLIL+R PKDF
Sbjct: 75  NKDKSMPWKKLDANEFGIQRSMIPDSTRMVLNKLKKKGFQVYLVGGCVRDLILDRIPKDF 134

Query: 120 DIITSAELKEVSRTFSWCEIVGMKFPICHVHIDGSIVEVSSFSTSSRPFDRHLDSAIEKP 179
           D+IT+AELKEV + F  C+IVG +FPICHV++D  I+EVSSFSTS+R   +  + +  +P
Sbjct: 135 DVITTAELKEVRKVFPGCQIVGRRFPICHVYVDDIIIEVSSFSTSART-GKAPNKSFRRP 194

Query: 180 MNCEEEDYVRWKNCLQRDFTINGLMYDPYNSAVYDYLEGMEDIKQAKIRTVVPACTSFQE 239
             C+E DY+RWKNCLQRDFT+NGLM+DP  + VYDY+ G+ED++ +K+RTV  A  SF E
Sbjct: 195 AGCDERDYIRWKNCLQRDFTVNGLMFDPSENVVYDYIGGVEDLRNSKVRTVSAANLSFVE 254

Query: 240 DCARILRAIRVAARLRFNFAKDTAGSIKKLSCLVSTLNKARLQMEMNYLLSYGSAEASLR 299
           D ARILRAIR+AARL F+  KD A S+K+LS  +  L+ +R++ME+NY+L+YGSAEASLR
Sbjct: 255 DTARILRAIRIAARLGFSLTKDVAISVKELSSSLLRLDPSRIRMEINYMLAYGSAEASLR 314

Query: 300 LLWKYGLLEILLPIQAAYFIQYGFRRSDKGSNMLLSLFSSMDKLLAPNRPCHSSLWVAVL 359
           LLW++GL+EILLPIQA+Y +  GFRR D  SNMLLSLF ++D+L+AP+RPC   LW+ +L
Sbjct: 315 LLWRFGLMEILLPIQASYLVSQGFRRRDGRSNMLLSLFRNLDRLVAPDRPCSEFLWIGIL 374

Query: 360 AFHVALSDQPRSPLVVAAFSLAVHNGGNMMEAISIAKSIGRAHNVNFHELLEPENLEVQT 419
           AFH AL DQPR P VVA+F LA+++  ++ EAI+IA+S  + HN +F EL  PE     +
Sbjct: 375 AFHKALVDQPRDPTVVASFCLAIYSEVSLSEAIAIARSNSKQHNSHFQELSSPEKDTADS 434

Query: 420 ---LIDEVMDLTTSIKNALHKMTDEHSVSLVLEMYPQAPASYLVFIPLVVYLKVRKIFEC 479
              +  +V+ L  SI++A  K+ +   ++  +  YPQAP S +VF+  ++  +V K+F  
Sbjct: 435 ESKISQQVIKLAESIRSAARKLNNRDYIANAMSKYPQAPGSDMVFLSRLMLERVEKMFGN 494

Query: 480 VVE--GAEGVFVPK-RGKVNYKCLALGDLPELRHVFARIVFDTVYPL 521
           V      E   VP    ++NYK LALGD  E R VFARIVFDT+YPL
Sbjct: 495 VRRKGNQERDDVPSLERRINYKSLALGDFHETRRVFARIVFDTIYPL 540

BLAST of CmaCh10G000280 vs. TAIR 10
Match: AT1G28090.2 (Polynucleotide adenylyltransferase family protein )

HSP 1 Score: 495.4 bits (1274), Expect = 5.8e-140
Identity = 252/467 (53.96%), Postives = 334/467 (71.52%), Query Frame = 0

Query: 60  NDSKLRKWKTFSSKELGIDIFMIPKPTRKVLNGLKKKGYEVYLVGGCVRDLILNRTPKDF 119
           N  K   WK   + E GI   MIP  TR VLN LKKKG++VYLVGGCVRDLIL+R PKDF
Sbjct: 39  NKDKSMPWKKLDANEFGIQRSMIPDSTRMVLNKLKKKGFQVYLVGGCVRDLILDRIPKDF 98

Query: 120 DIITSAELKEVSRTFSWCEIVGMKFPICHVHIDGSIVEVSSFSTSSRPFDRHLDSAIEKP 179
           D+IT+AELKEV + F  C+IVG +FPICHV++D  I+EVSSFSTS+R   +  + +  +P
Sbjct: 99  DVITTAELKEVRKVFPGCQIVGRRFPICHVYVDDIIIEVSSFSTSART-GKAPNKSFRRP 158

Query: 180 MNCEEEDYVRWKNCLQRDFTINGLMYDPYNSAVYDYLEGMEDIKQAKIRTVVPACTSFQE 239
             C+E DY+RWKNCLQRDFT+NGLM+DP  + VYDY+ G+ED++ +K+RTV  A  SF E
Sbjct: 159 AGCDERDYIRWKNCLQRDFTVNGLMFDPSENVVYDYIGGVEDLRNSKVRTVSAANLSFVE 218

Query: 240 DCARILRAIRVAARLRFNFAKDTAGSIKKLSCLVSTLNKARLQMEMNYLLSYGSAEASLR 299
           D ARILRAIR+AARL F+  KD A S+K+LS  +  L+ +R++ME+NY+L+YGSAEASLR
Sbjct: 219 DTARILRAIRIAARLGFSLTKDVAISVKELSSSLLRLDPSRIRMEINYMLAYGSAEASLR 278

Query: 300 LLWKYGLLEILLPIQAAYFIQYGFRRSDKGSNMLLSLFSSMDKLLAPNRPCHSSLWVAVL 359
           LLW++GL+EILLPIQA+Y +  GFRR D  SNMLLSLF ++D+L+AP+RPC   LW+ +L
Sbjct: 279 LLWRFGLMEILLPIQASYLVSQGFRRRDGRSNMLLSLFRNLDRLVAPDRPCSEFLWIGIL 338

Query: 360 AFHVALSDQPRSPLVVAAFSLAVHNGGNMMEAISIAKSIGRAHNVNFHELLEPENLEVQT 419
           AFH AL DQPR P VVA+F LA+++  ++ EAI+IA+S  + HN +F EL  PE     +
Sbjct: 339 AFHKALVDQPRDPTVVASFCLAIYSEVSLSEAIAIARSNSKQHNSHFQELSSPEKDTADS 398

Query: 420 ---LIDEVMDLTTSIKNALHKMTDEHSVSLVLEMYPQAPASYLVFIPLVVYLKVRKIFEC 479
              +  +V+ L  SI++A  K+ +   ++  +  YPQAP S +VF+  ++  +V K+F  
Sbjct: 399 ESKISQQVIKLAESIRSAARKLNNRDYIANAMSKYPQAPGSDMVFLSRLMLERVEKMFGN 458

Query: 480 VVE--GAEGVFVPK-RGKVNYKCLALGDLPELRHVFARIVFDTVYPL 521
           V      E   VP    ++NYK LALGD  E R VFARIVFDT+YPL
Sbjct: 459 VRRKGNQERDDVPSLERRINYKSLALGDFHETRRVFARIVFDTIYPL 504

BLAST of CmaCh10G000280 vs. TAIR 10
Match: AT1G28090.3 (Polynucleotide adenylyltransferase family protein )

HSP 1 Score: 495.4 bits (1274), Expect = 5.8e-140
Identity = 252/467 (53.96%), Postives = 334/467 (71.52%), Query Frame = 0

Query: 60  NDSKLRKWKTFSSKELGIDIFMIPKPTRKVLNGLKKKGYEVYLVGGCVRDLILNRTPKDF 119
           N  K   WK   + E GI   MIP  TR VLN LKKKG++VYLVGGCVRDLIL+R PKDF
Sbjct: 12  NKDKSMPWKKLDANEFGIQRSMIPDSTRMVLNKLKKKGFQVYLVGGCVRDLILDRIPKDF 71

Query: 120 DIITSAELKEVSRTFSWCEIVGMKFPICHVHIDGSIVEVSSFSTSSRPFDRHLDSAIEKP 179
           D+IT+AELKEV + F  C+IVG +FPICHV++D  I+EVSSFSTS+R   +  + +  +P
Sbjct: 72  DVITTAELKEVRKVFPGCQIVGRRFPICHVYVDDIIIEVSSFSTSART-GKAPNKSFRRP 131

Query: 180 MNCEEEDYVRWKNCLQRDFTINGLMYDPYNSAVYDYLEGMEDIKQAKIRTVVPACTSFQE 239
             C+E DY+RWKNCLQRDFT+NGLM+DP  + VYDY+ G+ED++ +K+RTV  A  SF E
Sbjct: 132 AGCDERDYIRWKNCLQRDFTVNGLMFDPSENVVYDYIGGVEDLRNSKVRTVSAANLSFVE 191

Query: 240 DCARILRAIRVAARLRFNFAKDTAGSIKKLSCLVSTLNKARLQMEMNYLLSYGSAEASLR 299
           D ARILRAIR+AARL F+  KD A S+K+LS  +  L+ +R++ME+NY+L+YGSAEASLR
Sbjct: 192 DTARILRAIRIAARLGFSLTKDVAISVKELSSSLLRLDPSRIRMEINYMLAYGSAEASLR 251

Query: 300 LLWKYGLLEILLPIQAAYFIQYGFRRSDKGSNMLLSLFSSMDKLLAPNRPCHSSLWVAVL 359
           LLW++GL+EILLPIQA+Y +  GFRR D  SNMLLSLF ++D+L+AP+RPC   LW+ +L
Sbjct: 252 LLWRFGLMEILLPIQASYLVSQGFRRRDGRSNMLLSLFRNLDRLVAPDRPCSEFLWIGIL 311

Query: 360 AFHVALSDQPRSPLVVAAFSLAVHNGGNMMEAISIAKSIGRAHNVNFHELLEPENLEVQT 419
           AFH AL DQPR P VVA+F LA+++  ++ EAI+IA+S  + HN +F EL  PE     +
Sbjct: 312 AFHKALVDQPRDPTVVASFCLAIYSEVSLSEAIAIARSNSKQHNSHFQELSSPEKDTADS 371

Query: 420 ---LIDEVMDLTTSIKNALHKMTDEHSVSLVLEMYPQAPASYLVFIPLVVYLKVRKIFEC 479
              +  +V+ L  SI++A  K+ +   ++  +  YPQAP S +VF+  ++  +V K+F  
Sbjct: 372 ESKISQQVIKLAESIRSAARKLNNRDYIANAMSKYPQAPGSDMVFLSRLMLERVEKMFGN 431

Query: 480 VVE--GAEGVFVPK-RGKVNYKCLALGDLPELRHVFARIVFDTVYPL 521
           V      E   VP    ++NYK LALGD  E R VFARIVFDT+YPL
Sbjct: 432 VRRKGNQERDDVPSLERRINYKSLALGDFHETRRVFARIVFDTIYPL 477

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P444394.1e-3733.46Poly(A) polymerase I OS=Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / ... [more]
Q8ZRQ81.6e-3337.30Poly(A) polymerase I OS=Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700... [more]
Q8Z9C32.7e-3337.30Poly(A) polymerase I OS=Salmonella typhi OX=90370 GN=pcnB PE=3 SV=2[more]
P0ABF34.7e-3336.89Poly(A) polymerase I OS=Escherichia coli O157:H7 OX=83334 GN=pcnB PE=3 SV=2[more]
P0ABF24.7e-3336.89Poly(A) polymerase I OS=Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UP... [more]
Match NameE-valueIdentityDescription
A0A6J1JFZ72.4e-306100.00uncharacterized protein LOC111484780 isoform X4 OS=Cucurbita maxima OX=3661 GN=L... [more]
A0A6J1JIL37.1e-30396.52uncharacterized protein LOC111484780 isoform X2 OS=Cucurbita maxima OX=3661 GN=L... [more]
A0A6J1JI152.7e-30297.77uncharacterized protein LOC111484780 isoform X3 OS=Cucurbita maxima OX=3661 GN=L... [more]
A0A6J1J9J18.2e-29994.43uncharacterized protein LOC111484780 isoform X1 OS=Cucurbita maxima OX=3661 GN=L... [more]
A0A6J1E1V41.0e-29697.53uncharacterized protein LOC111429822 isoform X4 OS=Cucurbita moschata OX=3662 GN... [more]
Match NameE-valueIdentityDescription
XP_022987140.14.9e-306100.00uncharacterized protein LOC111484780 isoform X4 [Cucurbita maxima][more]
XP_022987138.11.5e-30296.52uncharacterized protein LOC111484780 isoform X2 [Cucurbita maxima][more]
XP_022987139.15.6e-30297.77uncharacterized protein LOC111484780 isoform X3 [Cucurbita maxima][more]
XP_023516570.13.4e-29998.10uncharacterized protein LOC111780413 isoform X4 [Cucurbita pepo subsp. pepo][more]
XP_022987137.11.7e-29894.43uncharacterized protein LOC111484780 isoform X1 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
AT5G23690.11.4e-16563.50Polynucleotide adenylyltransferase family protein [more]
AT3G48830.11.9e-15457.76polynucleotide adenylyltransferase family protein / RNA recognition motif (RRM)-... [more]
AT1G28090.15.8e-14053.96Polynucleotide adenylyltransferase family protein [more]
AT1G28090.25.8e-14053.96Polynucleotide adenylyltransferase family protein [more]
AT1G28090.35.8e-14053.96Polynucleotide adenylyltransferase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D1.10.3090.10coord: 229..437
e-value: 9.7E-29
score: 102.7
NoneNo IPR availablePANTHERPTHR43051:SF2POLYNUCLEOTIDE ADENYLYLTRANSFERASE FAMILY PROTEIN-RELATEDcoord: 10..521
NoneNo IPR availablePANTHERPTHR43051POLYNUCLEOTIDE ADENYLYLTRANSFERASE FAMILY PROTEINcoord: 10..521
NoneNo IPR availableSUPERFAMILY81891Poly A polymerase C-terminal region-likecoord: 226..436
IPR002646Poly A polymerase, head domainPFAMPF01743PolyA_polcoord: 100..228
e-value: 3.7E-24
score: 85.5
IPR002646Poly A polymerase, head domainCDDcd05398NT_ClassII-CCAasecoord: 83..224
e-value: 1.63611E-34
score: 125.013
IPR032828tRNA nucleotidyltransferase/poly(A) polymerase, RNA and SrmB- binding domainPFAMPF12627PolyA_pol_RNAbdcoord: 256..316
e-value: 1.2E-14
score: 53.8
IPR043519Nucleotidyltransferase superfamilyGENE3D3.30.460.10Beta Polymerase, domain 2coord: 82..228
e-value: 1.4E-40
score: 140.3
IPR043519Nucleotidyltransferase superfamilySUPERFAMILY81301Nucleotidyltransferasecoord: 80..224

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh10G000280.1CmaCh10G000280.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0001680 tRNA 3'-terminal CCA addition
biological_process GO:0006396 RNA processing
molecular_function GO:0016779 nucleotidyltransferase activity
molecular_function GO:0003723 RNA binding