CmaCh09G012310 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh09G012310
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Descriptionregulator of nonsense transcripts UPF3-like
LocationCma_Chr09: 8208182 .. 8213663 (-)
RNA-Seq ExpressionCmaCh09G012310
SyntenyCmaCh09G012310
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
CCCCAATTCCCAAATTTCCCGAAAACATTTTCTCCTCCAGAGCAAAAATTGTCGAGAAATTAGAATCAAAAAGCAGAAAGAAATTCTGAATTTCTCTTGGCAGTGCGGGGGGTTTTTCAGATGAAGGACCCGTTGGAGCGGACCAAAGTAGTAATTAGGCACTTGCCCCCTTCTCTTCCTCACTCAGATCTCTTCCACAATATTGATGATCGATTCGCTGGTCGCTACAATTGGTGTTACTATCGTCCTGGAAAGACCAGGTTCGCCCCTCTTCTTCTTCTTCTTCTTCTCCTTCTTTTCCTCTAATTCCAGCTTTCAAACGAAAAATTAGTCTTATAAACTGGGTTTTCAGTTATATGTTGTTATATGTTGTTATATGTCGTCTTGGATTTCAGAAATCTGGAGATTGAATGAGTAACGCCATGCGCGAAGTGGGTATCTCTTGGATTGAGTTTTTGTGTGCTTTACGCTCTTTTCCCCCCCTTAGGTGGGCTATTGGGGAGGGTTGGTGATTAGAATAATGTAGGAGACTTTTTTCTCCCCTCCAATTTCATGCCCAAATGTGTATGATTATGATTCTGGAAACGAAATCCAGCAATCGTTCTAGAGTTCGTGTGTGTTTTTGGTTGGAATCGGCTAAGTTCTTACTTTCTCATGTTGTGTACTTTCTCTTGGTTTCTAAAGCTCACAATACTGAAGGTTTTTTACTCATTCAGAGGTTTCTTCTGAAATAATTTTGCAGTCAGAAGGACCAGAGATATGCTCGAGCTTACATAGATTTTACGAGACCTGAAGATGTTTTTGAGTTTGCGGAGTTTTTTGATGGGCATGTTTTCGTTAATGAGAAGGGTAAAGCCGATAGCTTGTTCTTTTTCCTTTATTGAACTTATGAGTAGCTTCTTGTTTCTTGTGGGTGTGGTGGGTGGTTCATTGAAACCTTACCCATTTCTTAGAAGTGGGAGATAACAAATTTATAAATGATTCCATTGCTTAATGTTGATTGATATGCTTACAGATTGCTCCCTTTGAGGTTTTCTGATGTGCATTACCCATTTCTAAGAACTGTTTAATGCTTGGATCATGCTTCTCTCCATATGTTTAAAGTCTCGATTGAAGTTAACTTATTTAGTCAAATAATTACAGGTGCTCAGTTTAAGGCTGTAGTTGAATATGCACCTTCACAGCGTGTTCCCAGATCATCGACCAAAAAGGATGGTCGTGAGGGGACTATATACAAAGGTAATGATCGGTTAGTGAGAAGACGGAGATTACATGGTTTTTCTTGTTTTATATTGGTTATTATGTAAGTGTAAATTATCTTTTCTCGATGAAAGCAGATCCGGATTATTTGGAGTTTCTCAAACTTATTGCAAAGCCTGCTGAGCATCTTCCAAGTGCTGAAATACAGTTGGAAAGAAAAGAAGCAGAGCAATCTGGTTTGTTATTATGTTCACTGACTGACTTATTGACATTACTCGCGTTCGTATATGTTTATAATGTAAAATAGGGTGCCGAAAAGTTGCTGAATACTCGTTATGTTACTTTGCTATGAGCTTCCATAGAGATACTATTCACTTTAAATTCAGATGATTCTTGAAGTTTTATATTTCTTCTTTTGTTTGCAGGTGCTGCGAAAGAAACTCCGATCGTTACCCCTCTCATGGAGTTTGTTCGCCAGAAACGAGCAGTTGAGAGCGGAACGCAGGTGAGATTGCTGTATTCTTAATACATGGTCCTGTGTGCTATATTTTAATCATTCAGAGAAAATGAAACCACTTTCTTTATTTTTTGTACTTCATTAGGTGTTGGTTCTGTCCATGTAGAGATTATTATTTATACATTCACTTCAGCATTAAGTCGAGATTCCTTCATTGGAACTCGTCTGCTGCTTGTGTAATAATTTCAGTATCTTTTCCTTTCGTATTGGTTAATACCATGCATTATGTGAATCCAAATGCTCTTACGATGGTTTTCTATGTGAGCTTAAAGATTGTATTCCTCTTCTAATTTATATATACCAATTCCTCATTATCTCATGTAAAGTTTTTTTTTGGTTCAATAGGGATCTTCATTACCTCGGAAGGTCAAAAGAGGTGGGGGAGCATCCTCTAGAAAGCCTGAGTCGAATTCCGCAAAGCGAGGGATGGAGAAGAAGAAGGCATGTGCTACTTTTTCATAGTTTTTTTCTTCGAACTTTATAAAATTCGCAAGTGCTAGTTTGTTAGTGTCTCCACTATTTTTCCCCTGTTGATTTTGGGAAAATAAGCTTTCTTTGCTTGTTAGATCTAAGGATGGGGAGCAGAATGTTTTTAACTAACAAAATGAAGAAAAATGCCTAAAGGATAATTACAAAAGTCCTTACTTACACTTGCTTAAAGAGATGCATTAAACCTAGCAAAGGACCCAATCTCCTCACGAAACGTTAAAGATTCTCATATTACTCTCTAGCGATCCATAAAATGACAAAAATCTAATGAAATATTTTAATATTGTACGCTTTGATCACAGAAGACCACTATCTCTATTACAAACCAAGCAACTCTTAAGACCTCAACCAAAATTGGAAATCAAGGTAGCAAACTGCCATCTCCCAAGGATGCGACTAAATCCTCAAATGCTCCCCTTCTAAGAATGCCTTGGTACAGCCATAACACAAAGAAATATTGCCTCTGTATTTCATCTATAGTGTTGACGCCCTAGTGCAAGTCCTTACTCCTAGTCCTGCAGCTCTAGAAACTTGACCTTGGAAATTTGTACCTTCGACAGTGAGGTGAAGCAGAAACTAATGATATCAATGGGTTTGACTGCAGAAACCAACCTAAAGTTTCTAATGTTATTGGTGGAAATAGTATAAATTTTTAATTTTTGCCTTACTGTGGTGTAAACTGTATCATGCTACACCACGTCGAGGTTATGATCAGTGAACATTGAAATTAAGGCTTTAGTTTTTTGCCCCCTACATTTCATACACTTAATTGATGCCCTTTTTTATGAACAACTTGTGTCTATATTTTTGTCTGAAGACTTTAGTTCTGTTATACATGCAGTATATTTTAAAGGAGAGTGTGAAGAATACAAATCGCAGAGACAAGTCAAATTTCATTTTGGTACCCAGGCGAGAGGATCAGTTAGCTACCTCAGGTGGAATTGGAATTTCTGATGTTGGTATAGGTATTTCTATTTAGATTTCTTTGTCGTCAGCGATATCATTTCTGTCATTTTTGTTTTGTTCTAGGTTATTGATCCGATATTTTATTGTTAGGCCATTGCAAAATACTATCTTGTTTCATGTCATTAGGAGCAGGAGAACACGAGACTATTTGATTTAAGATCTGAACACTTTATGCACTACTCCGCTGCTAGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTGTCAAGAGTTCCCACAACCATTTTATATTTTTCCCATTTCAAATTACCCTGAGCCGAGTAGTAATGTTCGTGGAAATTTCATTTTTCACTTGATTAATAATTATTTTCTTGTTTCTTTCTGGTATTTTGCCATATTGTGCTGTTGCAGATTGCAGGATTGTAATATGTGAACATGCATTATTAAGTTACCTTAAACACTTGTAAGTGATATATTTGAGGGATGGGAGGGGGCGCTTTTGTGATGTTGTATCAAATTTTGTCCATGAGGAAAAGGAAAAAATAAAACAAGGTCGTCCATTAGATAGTGCACATTGTCTGAAATTTTGAATTATGTTCTTTCCCTGCACTGGACTTCGCTACACATTTATTTCTTTTAATTTATCAATATGCTCTACATGTTTATAATGAACTTGCAGTCTATAATTTGAAGCCTCTGTTTCATATGTATCTGTAACAGCTGACTCTGGAAAGAAAAAAATTCTGCTTCTCAAGGGGAAAGAGCGAGATATATCTCATGTACGTTTCTTTAGTTCTCTCAACTAATTATTCCATTCCAACTTTGTTACTCTCTTTAGAATTATTAACTTGTGTGTATCAATTGTCACTCAAGGCTAATTTTGGAGGGCACAAATTCTTTTCTCTTGCCTCAAATTGTGACACTTGATTATCTATTTCTTGGATAATGATACCTTTGCAGGTTTCTGATGGCATGCTACAGTTGCAGAGCGTGACATCTACTGGGACTTCTCCTGCTAGTGCATCAAAACATAATCAGAGGCGTGAGGCTAGTGGAAGCATGATCAGAAGCATTCTTTTAAATAATGAATCACGTCATGGACAGACTTCATCAGGAGCTCCATCTCATCAAAAAATTCAGATCCTGAACTCAGACAATGGGAAGCGACTACCTCGTTCCATTAATGCTCGATCTGGGTCTAATGATATGTCCAGTAATGAACCAAATTCATCCGGTTTGGAAGGGGATGGAAAAAGGCCTTCAGACGGTAAACCTAGTAAGAAGGAACTTCATGGCCTGGGTAGTCTTAGCGAGAAGCAAGAAAAACGAATAAGAAACAAAGATAGACCTGATCGTGGTGTGTGGGCGCCACGTAGTCGTTCAGATGCTTCAGTCTCACAACTTGAGGAATCCTCTGTTGCACAATCTAGCCATTTGCTTTCTGACTCAGTTGAAGGTAGTACTATTATAATCCTTTGGAGATGAAGAGGCTACTTGCATATATATACACAATCATATTATTGAAGTATCGGACCTTGGTCATTGTGTCAAATCATACTGCTTGATATGAGCTAAATGCAGACTCTGTTCCACATGAGAAATTATAACCCAATATGTATTTTATGCATTTACAGCATACCGTGGGGAAATGAAAGAAGATATTCATGTAAGCAGGACTGGGGATGTTACAACTACTGTGAGTGGACGTAACAGTTCAGTGGAAAATGGTTGTACTTCCTCACGAACGCTTCTTACGTTCACTTATTTTAGGAAGCTGTAAAAGTACTTTCTGCTTTTCGTTAATCTAAAATGTGTTGTGCTTTCTTTAGGTTCTGTTAGACATGGTGGACGTCGTGGGGCTGGGCATGTCATGAAGGATGATGGGTCATTGAACCCAAATGAAGGGAAGCCGTCCAAAAGAGGTGTTGCCAGTGGTCACGAGGTTCGTTTCTGCATCCATGCTAGTGACTATCTCTCGTGTTACATGATCACTTCTTTTTCAGAAACTCATTATCTTTGATGCCTATGCAGAAACAAGTATGGGTTCAGAAGTCATCTTCAGGTTCTTAA

mRNA sequence

CCCCAATTCCCAAATTTCCCGAAAACATTTTCTCCTCCAGAGCAAAAATTGTCGAGAAATTAGAATCAAAAAGCAGAAAGAAATTCTGAATTTCTCTTGGCAGTGCGGGGGGTTTTTCAGATGAAGGACCCGTTGGAGCGGACCAAAGTAGTAATTAGGCACTTGCCCCCTTCTCTTCCTCACTCAGATCTCTTCCACAATATTGATGATCGATTCGCTGGTCGCTACAATTGGTGTTACTATCGTCCTGGAAAGACCAGTCAGAAGGACCAGAGATATGCTCGAGCTTACATAGATTTTACGAGACCTGAAGATGTTTTTGAGTTTGCGGAGTTTTTTGATGGGCATGTTTTCGTTAATGAGAAGGGTGCTCAGTTTAAGGCTGTAGTTGAATATGCACCTTCACAGCGTGTTCCCAGATCATCGACCAAAAAGGATGGTCGTGAGGGGACTATATACAAAGATCCGGATTATTTGGAGTTTCTCAAACTTATTGCAAAGCCTGCTGAGCATCTTCCAAGTGCTGAAATACAGTTGGAAAGAAAAGAAGCAGAGCAATCTGGTGCTGCGAAAGAAACTCCGATCGTTACCCCTCTCATGGAGTTTGTTCGCCAGAAACGAGCAGTTGAGAGCGGAACGCAGGGATCTTCATTACCTCGGAAGGTCAAAAGAGGTGGGGGAGCATCCTCTAGAAAGCCTGAGTCGAATTCCGCAAAGCGAGGGATGGAGAAGAAGAAGTATATTTTAAAGGAGAGTGTGAAGAATACAAATCGCAGAGACAAGTCAAATTTCATTTTGGTACCCAGGCGAGAGGATCAGTTAGCTACCTCAGGTGGAATTGGAATTTCTGATGTTGGTATAGCTGACTCTGGAAAGAAAAAAATTCTGCTTCTCAAGGGGAAAGAGCGAGATATATCTCATGTTTCTGATGGCATGCTACAGTTGCAGAGCGTGACATCTACTGGGACTTCTCCTGCTAGTGCATCAAAACATAATCAGAGGCGTGAGGCTAGTGGAAGCATGATCAGAAGCATTCTTTTAAATAATGAATCACGTCATGGACAGACTTCATCAGGAGCTCCATCTCATCAAAAAATTCAGATCCTGAACTCAGACAATGGGAAGCGACTACCTCGTTCCATTAATGCTCGATCTGGGTCTAATGATATGTCCAGTAATGAACCAAATTCATCCGGTTTGGAAGGGGATGGAAAAAGGCCTTCAGACGGTAAACCTAGTAAGAAGGAACTTCATGGCCTGGGTAGTCTTAGCGAGAAGCAAGAAAAACGAATAAGAAACAAAGATAGACCTGATCGTGGTGTGTGGGCGCCACGTAGTCGTTCAGATGCTTCAGTCTCACAACTTGAGGAATCCTCTGTTGCACAATCTAGCCATTTGCTTTCTGACTCAGTTGAAGCATACCGTGGGGAAATGAAAGAAGATATTCATGTAAGCAGGACTGGGGATGTTACAACTACTGTGAGTGGACGTAACAGTTCAGTGGAAAATGGTTCTGTTAGACATGGTGGACGTCGTGGGGCTGGGCATGTCATGAAGGATGATGGGTCATTGAACCCAAATGAAGGGAAGCCGTCCAAAAGAGGTGTTGCCAGTGGTCACGAGAAACAAGTATGGGTTCAGAAGTCATCTTCAGGTTCTTAA

Coding sequence (CDS)

ATGAAGGACCCGTTGGAGCGGACCAAAGTAGTAATTAGGCACTTGCCCCCTTCTCTTCCTCACTCAGATCTCTTCCACAATATTGATGATCGATTCGCTGGTCGCTACAATTGGTGTTACTATCGTCCTGGAAAGACCAGTCAGAAGGACCAGAGATATGCTCGAGCTTACATAGATTTTACGAGACCTGAAGATGTTTTTGAGTTTGCGGAGTTTTTTGATGGGCATGTTTTCGTTAATGAGAAGGGTGCTCAGTTTAAGGCTGTAGTTGAATATGCACCTTCACAGCGTGTTCCCAGATCATCGACCAAAAAGGATGGTCGTGAGGGGACTATATACAAAGATCCGGATTATTTGGAGTTTCTCAAACTTATTGCAAAGCCTGCTGAGCATCTTCCAAGTGCTGAAATACAGTTGGAAAGAAAAGAAGCAGAGCAATCTGGTGCTGCGAAAGAAACTCCGATCGTTACCCCTCTCATGGAGTTTGTTCGCCAGAAACGAGCAGTTGAGAGCGGAACGCAGGGATCTTCATTACCTCGGAAGGTCAAAAGAGGTGGGGGAGCATCCTCTAGAAAGCCTGAGTCGAATTCCGCAAAGCGAGGGATGGAGAAGAAGAAGTATATTTTAAAGGAGAGTGTGAAGAATACAAATCGCAGAGACAAGTCAAATTTCATTTTGGTACCCAGGCGAGAGGATCAGTTAGCTACCTCAGGTGGAATTGGAATTTCTGATGTTGGTATAGCTGACTCTGGAAAGAAAAAAATTCTGCTTCTCAAGGGGAAAGAGCGAGATATATCTCATGTTTCTGATGGCATGCTACAGTTGCAGAGCGTGACATCTACTGGGACTTCTCCTGCTAGTGCATCAAAACATAATCAGAGGCGTGAGGCTAGTGGAAGCATGATCAGAAGCATTCTTTTAAATAATGAATCACGTCATGGACAGACTTCATCAGGAGCTCCATCTCATCAAAAAATTCAGATCCTGAACTCAGACAATGGGAAGCGACTACCTCGTTCCATTAATGCTCGATCTGGGTCTAATGATATGTCCAGTAATGAACCAAATTCATCCGGTTTGGAAGGGGATGGAAAAAGGCCTTCAGACGGTAAACCTAGTAAGAAGGAACTTCATGGCCTGGGTAGTCTTAGCGAGAAGCAAGAAAAACGAATAAGAAACAAAGATAGACCTGATCGTGGTGTGTGGGCGCCACGTAGTCGTTCAGATGCTTCAGTCTCACAACTTGAGGAATCCTCTGTTGCACAATCTAGCCATTTGCTTTCTGACTCAGTTGAAGCATACCGTGGGGAAATGAAAGAAGATATTCATGTAAGCAGGACTGGGGATGTTACAACTACTGTGAGTGGACGTAACAGTTCAGTGGAAAATGGTTCTGTTAGACATGGTGGACGTCGTGGGGCTGGGCATGTCATGAAGGATGATGGGTCATTGAACCCAAATGAAGGGAAGCCGTCCAAAAGAGGTGTTGCCAGTGGTCACGAGAAACAAGTATGGGTTCAGAAGTCATCTTCAGGTTCTTAA

Protein sequence

MKDPLERTKVVIRHLPPSLPHSDLFHNIDDRFAGRYNWCYYRPGKTSQKDQRYARAYIDFTRPEDVFEFAEFFDGHVFVNEKGAQFKAVVEYAPSQRVPRSSTKKDGREGTIYKDPDYLEFLKLIAKPAEHLPSAEIQLERKEAEQSGAAKETPIVTPLMEFVRQKRAVESGTQGSSLPRKVKRGGGASSRKPESNSAKRGMEKKKYILKESVKNTNRRDKSNFILVPRREDQLATSGGIGISDVGIADSGKKKILLLKGKERDISHVSDGMLQLQSVTSTGTSPASASKHNQRREASGSMIRSILLNNESRHGQTSSGAPSHQKIQILNSDNGKRLPRSINARSGSNDMSSNEPNSSGLEGDGKRPSDGKPSKKELHGLGSLSEKQEKRIRNKDRPDRGVWAPRSRSDASVSQLEESSVAQSSHLLSDSVEAYRGEMKEDIHVSRTGDVTTTVSGRNSSVENGSVRHGGRRGAGHVMKDDGSLNPNEGKPSKRGVASGHEKQVWVQKSSSGS
Homology
BLAST of CmaCh09G012310 vs. ExPASy Swiss-Prot
Match: Q9FVW4 (Regulator of nonsense transcripts UPF3 OS=Arabidopsis thaliana OX=3702 GN=UPF3 PE=1 SV=1)

HSP 1 Score: 394.4 bits (1012), Expect = 1.9e-108
Identity = 250/530 (47.17%), Postives = 323/530 (60.94%), Query Frame = 0

Query: 1   MKDPLERTKVVIRHLPPSLPHSDLFHNIDDRFAGRYNWCYYRPGKTSQKDQRYARAYIDF 60
           MK+PL++ KVV+RHLPPSL  SDL   ID RFA RYNW  +RPGK+S K+Q+Y+RAY+ F
Sbjct: 1   MKEPLQKKKVVVRHLPPSLSQSDLLSQIDPRFADRYNWVSFRPGKSSYKNQKYSRAYVSF 60

Query: 61  TRPEDVFEFAEFFDGHVFVNEKGAQFKAVVEYAPSQRVPRSSTKKDGREGTIYKDPDYLE 120
             PEDV+EFA FF+GHVFVNEKGAQFKA+VEYAPSQRVP+ S KKD REG+I KDPDYLE
Sbjct: 61  KAPEDVYEFAAFFNGHVFVNEKGAQFKAIVEYAPSQRVPKPSDKKDPREGSISKDPDYLE 120

Query: 121 FLKLIAKPAEHLPSAEIQLERKEAEQSGAAKETPIVTPLMEFVRQKRAVESGTQG-SSLP 180
           FLK+IA+P E+LPSAEIQLER+EAEQSGA+K  PIVTPLMEF+RQKRA   G QG S + 
Sbjct: 121 FLKVIAQPVENLPSAEIQLERREAEQSGASKAAPIVTPLMEFIRQKRATVMGPQGLSDIR 180

Query: 181 RKVKRGGGASSRKPESNSAKRGMEKKKYILKESVKNTNRRDKSNFILVPRREDQLATSGG 240
           R  +R    S+ KP    +KR  EKKKY+ KES KN  R+  ++         Q  +SG 
Sbjct: 181 RGGRRTRVVSANKPSPRPSKRNSEKKKYVEKESSKNVPRKTTADVSSSKPDYRQSNSSGK 240

Query: 241 ----------IGISDVGIA---DSGKKKILLLKGKERDISHVSDGMLQLQSVTSTGTSPA 300
                     I  S  GIA   DSGKKKILLL+ K+RD         + Q + +  +  +
Sbjct: 241 ELPGNETAAIIDSSPPGIALTMDSGKKKILLLRSKDRDNPDNPPPQPE-QHIDTNLSRNS 300

Query: 301 SASKHNQRREASGSMIRSILLNNESRHGQTSSGAPSHQKIQILNSDNGKRLPRSINARSG 360
           + S+ NQ+ +  G +I+ ILL N+SR  Q+S+   S Q+++   ++N KR  R  N R+G
Sbjct: 301 TDSRQNQKSDVGGRLIKGILLRNDSRPSQSSTFVQSEQRVEPSEAENYKRPSRPANTRAG 360

Query: 361 SNDMSSNEPNSSGLEGDGKRPSDGKPSKKELHGLGSLSEKQEKRIRNKDRPDRGVWAPRS 420
                                       K+ H  G++SEKQE+R RNKDRPDR +WAP  
Sbjct: 361 ----------------------------KDYHTSGTISEKQERRTRNKDRPDRVMWAP-- 420

Query: 421 RSDASVSQLEESSVAQSSHLLSDSVEAYRGEMKEDIHVSRTGDVTTTVSGRNSSVENGSV 480
           R D S  Q   S+                GE+K+ +   R+G+V  +  G   ++ENGS 
Sbjct: 421 RRDGSEDQPLSSA-------------GNNGEVKDRMFSQRSGEVVNSSGGH--TLENGSA 480

Query: 481 RHGGRRGAGHVMKDDGSLNPNEGKPSKRGVASG---HEKQVWVQKSSSGS 514
           RH  RR  G   K++  +   EGK S+RG   G   HEKQ+W+QK SSG+
Sbjct: 481 RHSSRRVGGRNRKEEVVI--GEGKTSRRGSGGGPSSHEKQMWIQKPSSGT 482

BLAST of CmaCh09G012310 vs. ExPASy Swiss-Prot
Match: B0S733 (Regulator of nonsense transcripts 3A OS=Danio rerio OX=7955 GN=upf3a PE=1 SV=2)

HSP 1 Score: 95.9 bits (237), Expect = 1.4e-18
Identity = 72/224 (32.14%), Postives = 116/224 (51.79%), Query Frame = 0

Query: 8   TKVVIRHLPPSLPHSDLFHNIDDRFAGRYNWCYYRPGKTSQKDQRYARAYIDFTRPEDVF 67
           +KVVIR LPPSL    L  ++       +++  + P   S     ++RAYI+F  PED+ 
Sbjct: 43  SKVVIRRLPPSLSKDQLQEHLSP--LPSFDYFEFFPADQSLYPHLFSRAYINFKNPEDII 102

Query: 68  EFAEFFDGHVFVNEKGAQFKAVVEYAPSQRVPRSS-TKKDGREGTIYKDPDYLEFL---- 127
            F + FDG+VF++ KG ++ AVVE+AP Q+V +    KKD + GTI +DP+Y  FL    
Sbjct: 103 IFRDRFDGYVFIDNKGQEYPAVVEFAPFQKVSKKKLKKKDAKAGTIEEDPEYRRFLENYS 162

Query: 128 ----KLIAKPAEHLPSAEIQLERKEAEQSGAAKETPIVTPLMEFVRQKRAVESGTQGS-- 187
               K +A P   L   EI+ + +E      AK T   TPL+E+++ K+  +   +    
Sbjct: 163 CDEEKSMANPETLL--GEIEAKTREL----IAKRT---TPLLEYIKNKKLEKQRIREEKR 222

Query: 188 ------SLPRKVKRGGGASSRKPESNSAKRGMEKKKYILKESVK 215
                  L +K +R      R+ E    ++  EK+K + ++ +K
Sbjct: 223 EERRRRELEKKRQREEEKRKRREEERQKRKEAEKQKKLSEKEIK 255

BLAST of CmaCh09G012310 vs. ExPASy Swiss-Prot
Match: Q9BZI7 (Regulator of nonsense transcripts 3B OS=Homo sapiens OX=9606 GN=UPF3B PE=1 SV=1)

HSP 1 Score: 87.0 bits (214), Expect = 6.6e-16
Identity = 115/434 (26.50%), Postives = 187/434 (43.09%), Query Frame = 0

Query: 8   TKVVIRHLPPSLPHSDLFHNIDDRFAGRYNWCYYRPGKTSQKDQRYARAYIDFTRPEDVF 67
           +KVVIR LPP+L    L  ++       +++  +    TS     YARAYI+F   ED+ 
Sbjct: 51  SKVVIRRLPPTLTKEQLQEHLQP--MPEHDYFEFFSNDTSLYPHMYARAYINFKNQEDII 110

Query: 68  EFAEFFDGHVFVNEKGAQFKAVVEYAPSQRVPRSSTKK-DGREGTIYKDPDYLEFLKLIA 127
            F + FDG+VF++ KG ++ A+VE+AP Q+  +  TKK D + GTI  DP+Y +FL+  A
Sbjct: 111 LFRDRFDGYVFLDNKGQEYPAIVEFAPFQKAAKKKTKKRDTKVGTIDDDPEYRKFLESYA 170

Query: 128 KPAEHLPSA-EIQLERKEAEQSG--AAKETPIVTPLMEFVRQKRAVESGTQGSSLPRKVK 187
              E + S  E  LE  EA+     A K TP+++ L    R +       +   + RK +
Sbjct: 171 TDNEKMTSTPETLLEEIEAKNRELIAKKTTPLLSFLKNKQRMREEKREERRRREIERKRQ 230

Query: 188 RGGGASSRKPESNSAKRGMEKKKYI----LKESVKNTNRRDKSNFILVPRREDQLATSGG 247
           R       K E    ++ +EK K I     ++ +K+  +     F+L    +  L     
Sbjct: 231 REEERRKWKEEEKRKRKDIEKLKKIDRIPERDKLKDEPKIKVHRFLLQAVNQKNLLKK-- 290

Query: 248 IGISDVGIADSGKKKIL--LLKGKERDISHVSDGMLQLQSVTSTGTSPASASKHNQRR-- 307
                    + G +K L    K K+ D  ++SD     QS T    S +       +R  
Sbjct: 291 --------PEKGDEKELDKREKAKKLDKENLSDERASGQSCTLPKRSDSELKDEKPKRPE 350

Query: 308 EASGSMIRSILLNNESRHGQTSSGAPSHQKIQILNSDNGKRLPRSINARSGSNDMSSNEP 367
           + SG   R        R  +        ++ ++   +  +R  +    +  +      E 
Sbjct: 351 DESGRDYR-----EREREYERDQERILRERERLKRQEEERRRQKERYEKEKTFKRKEEEM 410

Query: 368 NSSGLEGDGKRPSDGKPSKKELHGLGSLSEKQEK-----RIRNKDRPDRGVWAPRSRSDA 425
                E D  R    K    E  G    +EK+E+     RIRNKDRP   ++ P +RS  
Sbjct: 411 KK---EKDTLRDKGKKAESTESIGSSEKTEKKEEVVKRDRIRNKDRPAMQLYQPGARSRN 464

BLAST of CmaCh09G012310 vs. ExPASy Swiss-Prot
Match: Q9H1J1 (Regulator of nonsense transcripts 3A OS=Homo sapiens OX=9606 GN=UPF3A PE=1 SV=1)

HSP 1 Score: 84.3 bits (207), Expect = 4.3e-15
Identity = 68/246 (27.64%), Postives = 116/246 (47.15%), Query Frame = 0

Query: 8   TKVVIRHLPPSLPHSDLFHNIDDRFAGRYNWCYYRPGKTSQKDQRYARAYIDFTRPEDVF 67
           +KVVIR LPP L    L   +  R    +++  +     S     Y+RAYI+F  P+D+ 
Sbjct: 68  SKVVIRRLPPGLTKEQLEEQL--RPLPAHDYFEFFAADLSLYPHLYSRAYINFRNPDDIL 127

Query: 68  EFAEFFDGHVFVNEKGAQFKAVVEYAPSQRVPRSS-TKKDGREGTIYKDPDYLEFLKLIA 127
            F + FDG++F++ KG ++ AVVE+AP Q++ +    KKD + G+I  DP+Y +FL+   
Sbjct: 128 LFRDRFDGYIFLDSKGLEYPAVVEFAPFQKIAKKKLRKKDAKTGSIEDDPEYKKFLETYC 187

Query: 128 KPAEHLPSAEIQLERKEAEQSGAAKETPIVTPLMEFVRQKRAVESGTQGS--------SL 187
              E   SA  +    E E           TPL+E+++ ++  +   +           L
Sbjct: 188 -VEEEKTSANPETLLGEMEAKTRELIARRTTPLLEYIKNRKLEKQRIREEKREERRRREL 247

Query: 188 PRKVKRGGGASSRKPESNSAKRGMEKKKYILKESV-----KNTNRRDKSNFILVPRREDQ 240
            +K  R      R+ E    K+  +K+K I ++ V     K   + ++        R ++
Sbjct: 248 EKKRLREEEKRRRREEERCKKKETDKQKKIAEKEVRIKLLKKPEKGEEPTTEKPKERGEE 307

BLAST of CmaCh09G012310 vs. ExPASy Swiss-Prot
Match: Q10267 (Nonsense-mediated mRNA decay protein 3 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=upf3 PE=3 SV=1)

HSP 1 Score: 72.0 bits (175), Expect = 2.2e-11
Identity = 52/202 (25.74%), Postives = 94/202 (46.53%), Query Frame = 0

Query: 9   KVVIRHLPPSLPHSDLFHNIDDRFAGRYNWCYYRPGKTS--QKDQRYARAYIDFTRPEDV 68
           KV++ +LPP+LP      +I+  F     W  +  GK +   + +  + AY+ F     V
Sbjct: 13  KVLVFNLPPTLPEQVFLQSINS-FLPHVEWHRFSKGKATVGTRSELLSFAYLKFQSATAV 72

Query: 69  FEFAEFFDGHVFVNEKGAQFKAVVEYAPSQRVPRSSTKKDGREGTIYKDPDYLEFLKLIA 128
            EF   + GH F+++K   ++A+V  AP Q++P S  K D  EG++ +DP + EF     
Sbjct: 73  QEFFRVYQGHTFIDKKNNTYRAIVTIAPYQKIPPSKVKADSLEGSLEQDPKFQEF----- 132

Query: 129 KPAEHLPSAEIQLERKEAEQSGAAKETPIVTPLMEFVRQKR--AVESGTQGSSLPRKVKR 188
                    + +   + A      ++    TPL++++ +K+   VE G    S      +
Sbjct: 133 -------KVQRESYSQTASNDDVIEKLQTSTPLLQYLAEKKNAVVEKGKSKPSKKSVKAK 192

Query: 189 GGGASSRKPESNSAKRGMEKKK 207
                + KP SN++K G   ++
Sbjct: 193 KKLRLAEKPASNNSKAGKSSQE 201

BLAST of CmaCh09G012310 vs. ExPASy TrEMBL
Match: A0A6J1IG63 (regulator of nonsense transcripts UPF3-like OS=Cucurbita maxima OX=3661 GN=LOC111472590 PE=3 SV=1)

HSP 1 Score: 981.5 bits (2536), Expect = 1.4e-282
Identity = 513/513 (100.00%), Postives = 513/513 (100.00%), Query Frame = 0

Query: 1   MKDPLERTKVVIRHLPPSLPHSDLFHNIDDRFAGRYNWCYYRPGKTSQKDQRYARAYIDF 60
           MKDPLERTKVVIRHLPPSLPHSDLFHNIDDRFAGRYNWCYYRPGKTSQKDQRYARAYIDF
Sbjct: 1   MKDPLERTKVVIRHLPPSLPHSDLFHNIDDRFAGRYNWCYYRPGKTSQKDQRYARAYIDF 60

Query: 61  TRPEDVFEFAEFFDGHVFVNEKGAQFKAVVEYAPSQRVPRSSTKKDGREGTIYKDPDYLE 120
           TRPEDVFEFAEFFDGHVFVNEKGAQFKAVVEYAPSQRVPRSSTKKDGREGTIYKDPDYLE
Sbjct: 61  TRPEDVFEFAEFFDGHVFVNEKGAQFKAVVEYAPSQRVPRSSTKKDGREGTIYKDPDYLE 120

Query: 121 FLKLIAKPAEHLPSAEIQLERKEAEQSGAAKETPIVTPLMEFVRQKRAVESGTQGSSLPR 180
           FLKLIAKPAEHLPSAEIQLERKEAEQSGAAKETPIVTPLMEFVRQKRAVESGTQGSSLPR
Sbjct: 121 FLKLIAKPAEHLPSAEIQLERKEAEQSGAAKETPIVTPLMEFVRQKRAVESGTQGSSLPR 180

Query: 181 KVKRGGGASSRKPESNSAKRGMEKKKYILKESVKNTNRRDKSNFILVPRREDQLATSGGI 240
           KVKRGGGASSRKPESNSAKRGMEKKKYILKESVKNTNRRDKSNFILVPRREDQLATSGGI
Sbjct: 181 KVKRGGGASSRKPESNSAKRGMEKKKYILKESVKNTNRRDKSNFILVPRREDQLATSGGI 240

Query: 241 GISDVGIADSGKKKILLLKGKERDISHVSDGMLQLQSVTSTGTSPASASKHNQRREASGS 300
           GISDVGIADSGKKKILLLKGKERDISHVSDGMLQLQSVTSTGTSPASASKHNQRREASGS
Sbjct: 241 GISDVGIADSGKKKILLLKGKERDISHVSDGMLQLQSVTSTGTSPASASKHNQRREASGS 300

Query: 301 MIRSILLNNESRHGQTSSGAPSHQKIQILNSDNGKRLPRSINARSGSNDMSSNEPNSSGL 360
           MIRSILLNNESRHGQTSSGAPSHQKIQILNSDNGKRLPRSINARSGSNDMSSNEPNSSGL
Sbjct: 301 MIRSILLNNESRHGQTSSGAPSHQKIQILNSDNGKRLPRSINARSGSNDMSSNEPNSSGL 360

Query: 361 EGDGKRPSDGKPSKKELHGLGSLSEKQEKRIRNKDRPDRGVWAPRSRSDASVSQLEESSV 420
           EGDGKRPSDGKPSKKELHGLGSLSEKQEKRIRNKDRPDRGVWAPRSRSDASVSQLEESSV
Sbjct: 361 EGDGKRPSDGKPSKKELHGLGSLSEKQEKRIRNKDRPDRGVWAPRSRSDASVSQLEESSV 420

Query: 421 AQSSHLLSDSVEAYRGEMKEDIHVSRTGDVTTTVSGRNSSVENGSVRHGGRRGAGHVMKD 480
           AQSSHLLSDSVEAYRGEMKEDIHVSRTGDVTTTVSGRNSSVENGSVRHGGRRGAGHVMKD
Sbjct: 421 AQSSHLLSDSVEAYRGEMKEDIHVSRTGDVTTTVSGRNSSVENGSVRHGGRRGAGHVMKD 480

Query: 481 DGSLNPNEGKPSKRGVASGHEKQVWVQKSSSGS 514
           DGSLNPNEGKPSKRGVASGHEKQVWVQKSSSGS
Sbjct: 481 DGSLNPNEGKPSKRGVASGHEKQVWVQKSSSGS 513

BLAST of CmaCh09G012310 vs. ExPASy TrEMBL
Match: A0A6J1EI29 (regulator of nonsense transcripts UPF3-like OS=Cucurbita moschata OX=3662 GN=LOC111432763 PE=3 SV=1)

HSP 1 Score: 963.8 bits (2490), Expect = 2.9e-277
Identity = 503/513 (98.05%), Postives = 506/513 (98.64%), Query Frame = 0

Query: 1   MKDPLERTKVVIRHLPPSLPHSDLFHNIDDRFAGRYNWCYYRPGKTSQKDQRYARAYIDF 60
           MKDPLERTKVVIRHLPPSLPHSDLFHNIDDRFAGRYNWCYYRPGKTSQKDQRYARAYIDF
Sbjct: 1   MKDPLERTKVVIRHLPPSLPHSDLFHNIDDRFAGRYNWCYYRPGKTSQKDQRYARAYIDF 60

Query: 61  TRPEDVFEFAEFFDGHVFVNEKGAQFKAVVEYAPSQRVPRSSTKKDGREGTIYKDPDYLE 120
           TRPEDVFEFAEFFDGHVFVNEKGAQFKAVVEYAPSQRVPRSSTKKDGREGTIYKDPDYLE
Sbjct: 61  TRPEDVFEFAEFFDGHVFVNEKGAQFKAVVEYAPSQRVPRSSTKKDGREGTIYKDPDYLE 120

Query: 121 FLKLIAKPAEHLPSAEIQLERKEAEQSGAAKETPIVTPLMEFVRQKRAVESGTQGSSLPR 180
           FLKLIAKPAEHLPSAEIQLERKEAEQS AAKETPIVTPLMEFVRQKRAVESGTQGSS+PR
Sbjct: 121 FLKLIAKPAEHLPSAEIQLERKEAEQSAAAKETPIVTPLMEFVRQKRAVESGTQGSSVPR 180

Query: 181 KVKRGGGASSRKPESNSAKRGMEKKKYILKESVKNTNRRDKSNFILVPRREDQLATSGGI 240
           KVKRGGGASSRKPESNS KRGMEKKKYILKESVKNTNRRDKSNFILVPRREDQLATSGGI
Sbjct: 181 KVKRGGGASSRKPESNSVKRGMEKKKYILKESVKNTNRRDKSNFILVPRREDQLATSGGI 240

Query: 241 GISDVGIADSGKKKILLLKGKERDISHVSDGMLQLQSVTSTGTSPASASKHNQRREASGS 300
           GISDVG ADSGKKKILLLKGKERDISHVSDGMLQLQS TS+GTSPASASKHNQRREASGS
Sbjct: 241 GISDVGTADSGKKKILLLKGKERDISHVSDGMLQLQSATSSGTSPASASKHNQRREASGS 300

Query: 301 MIRSILLNNESRHGQTSSGAPSHQKIQILNSDNGKRLPRSINARSGSNDMSSNEPNSSGL 360
           MIRSILLNNESRHGQTSSGAPSHQKIQILNSDNGKRLPRSINARSGSNDMSSNEPN SGL
Sbjct: 301 MIRSILLNNESRHGQTSSGAPSHQKIQILNSDNGKRLPRSINARSGSNDMSSNEPNPSGL 360

Query: 361 EGDGKRPSDGKPSKKELHGLGSLSEKQEKRIRNKDRPDRGVWAPRSRSDASVSQLEESSV 420
           EGDGKRPSDGKPSKKELHGLGSLSEKQEKRIRNKDRPDRGVWAPRSRSDASVSQLEESSV
Sbjct: 361 EGDGKRPSDGKPSKKELHGLGSLSEKQEKRIRNKDRPDRGVWAPRSRSDASVSQLEESSV 420

Query: 421 AQSSHLLSDSVEAYRGEMKEDIHVSRTGDVTTTVSGRNSSVENGSVRHGGRRGAGHVMKD 480
           AQSSHLLSDSVEAYRGEMKEDIHVSRTGDVTTTVSGR+SSVENGSVRHGGRRGAGHVMKD
Sbjct: 421 AQSSHLLSDSVEAYRGEMKEDIHVSRTGDVTTTVSGRSSSVENGSVRHGGRRGAGHVMKD 480

Query: 481 DGSLNPNEGKPSKRGVASGHEKQVWVQKSSSGS 514
           DGS NPNEGKPSKRGV SGHEKQVWVQKSSSGS
Sbjct: 481 DGSSNPNEGKPSKRGVVSGHEKQVWVQKSSSGS 513

BLAST of CmaCh09G012310 vs. ExPASy TrEMBL
Match: A0A0A0KSY5 (Smg4_UPF3 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G650630 PE=3 SV=1)

HSP 1 Score: 888.6 bits (2295), Expect = 1.2e-254
Identity = 470/513 (91.62%), Postives = 481/513 (93.76%), Query Frame = 0

Query: 1   MKDPLERTKVVIRHLPPSLPHSDLFHNIDDRFAGRYNWCYYRPGKTSQKDQRYARAYIDF 60
           MKDPLERTKVVIRHLPPSL HSDLFH+I DRFAGR+NW YYRPGKTSQKDQRYARAYIDF
Sbjct: 1   MKDPLERTKVVIRHLPPSLSHSDLFHHIHDRFAGRFNWSYYRPGKTSQKDQRYARAYIDF 60

Query: 61  TRPEDVFEFAEFFDGHVFVNEKGAQFKAVVEYAPSQRVPRSSTKKDGREGTIYKDPDYLE 120
           TRPEDVFEFAEFFDGHVFVNEKGAQ+KAVVEYAPSQRVPRSSTKKDGREGTIYKDPDYLE
Sbjct: 61  TRPEDVFEFAEFFDGHVFVNEKGAQYKAVVEYAPSQRVPRSSTKKDGREGTIYKDPDYLE 120

Query: 121 FLKLIAKPAEHLPSAEIQLERKEAEQSGAAKETPIVTPLMEFVRQKRAVESGTQGSSLPR 180
           FLKLIAKPAEHLPSAEIQLERKEAEQSGAAKETPIVTPLMEFVRQKRAVESGTQGSS+PR
Sbjct: 121 FLKLIAKPAEHLPSAEIQLERKEAEQSGAAKETPIVTPLMEFVRQKRAVESGTQGSSVPR 180

Query: 181 KVKRGGGASSRKPESNSAKRGMEKKKYILKESVKNTNRRDKSNFILVPRREDQLATSGGI 240
           KVKRGG ASSRKPESNS KRGMEKKKYILK+SVKNTNRRDKSNFILVPRREDQ ATS  I
Sbjct: 181 KVKRGGAASSRKPESNSMKRGMEKKKYILKDSVKNTNRRDKSNFILVPRREDQSATSSAI 240

Query: 241 GISDVGIADSGKKKILLLKGKERDISHVSDGMLQLQSVTSTGTSPASASKHNQRREASGS 300
           GISDVG AD GKKKILLLKGKERDISHVSD MLQLQS TS+G SPASASKHN RREA G 
Sbjct: 241 GISDVGTADFGKKKILLLKGKERDISHVSDDMLQLQSATSSGNSPASASKHNHRREAGGG 300

Query: 301 MIRSILLNNESRHGQTSSGAPSHQKIQILNSDNGKRLPRSINARSGSNDMSSNEPNSSGL 360
           +IRSILLNNE+RHGQ+SS A SHQKIQILNSDNGKR PR  NARSGSND+SSNEPN SG 
Sbjct: 301 VIRSILLNNEARHGQSSSVAQSHQKIQILNSDNGKRPPRPTNARSGSNDISSNEPNPSGS 360

Query: 361 EGDGKRPSDGKPSKKELHGLGSLSEKQEKRIRNKDRPDRGVWAPRSRSDASVSQLEESSV 420
           EGDGKR SD K SKKELHGLGS SEKQEKRIRNKDRPDRGVWAPRSRSDASVSQLEESSV
Sbjct: 361 EGDGKRASDNKFSKKELHGLGSASEKQEKRIRNKDRPDRGVWAPRSRSDASVSQLEESSV 420

Query: 421 AQSSHLLSDSVEAYRGEMKEDIHVSRTGDVTTTVSGRNSSVENGSVRHGGRRGAGHVMKD 480
            QSSHLLSDSVEA+RGEMKEDIH SRTGDVTT VSGRNSSVENGSVRH GRRGAGHVMKD
Sbjct: 421 PQSSHLLSDSVEAFRGEMKEDIHGSRTGDVTTIVSGRNSSVENGSVRHVGRRGAGHVMKD 480

Query: 481 DGSLNPNEGKPSKRGVASGHEKQVWVQKSSSGS 514
           DGSLNPNEGKPSKRGVA GHEKQVWVQKSSSGS
Sbjct: 481 DGSLNPNEGKPSKRGVAGGHEKQVWVQKSSSGS 513

BLAST of CmaCh09G012310 vs. ExPASy TrEMBL
Match: A0A1S3CB69 (regulator of nonsense transcripts UPF3 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103498712 PE=3 SV=1)

HSP 1 Score: 887.5 bits (2292), Expect = 2.7e-254
Identity = 469/513 (91.42%), Postives = 481/513 (93.76%), Query Frame = 0

Query: 1   MKDPLERTKVVIRHLPPSLPHSDLFHNIDDRFAGRYNWCYYRPGKTSQKDQRYARAYIDF 60
           MKDPLERTKVVIRHLPPSL HSDLFH+I DRFAGR+NW YYRPGKTSQKDQRYARAYIDF
Sbjct: 1   MKDPLERTKVVIRHLPPSLSHSDLFHHIHDRFAGRFNWSYYRPGKTSQKDQRYARAYIDF 60

Query: 61  TRPEDVFEFAEFFDGHVFVNEKGAQFKAVVEYAPSQRVPRSSTKKDGREGTIYKDPDYLE 120
           TRPEDVFEFAEFFDGHVFVNEKGAQ+KAVVEYAPSQRVPRSSTKKDGREGTIYKDPDYLE
Sbjct: 61  TRPEDVFEFAEFFDGHVFVNEKGAQYKAVVEYAPSQRVPRSSTKKDGREGTIYKDPDYLE 120

Query: 121 FLKLIAKPAEHLPSAEIQLERKEAEQSGAAKETPIVTPLMEFVRQKRAVESGTQGSSLPR 180
           FLKLIAKPAEHLPSAEIQLERKEAEQSGAAKETPIVTPLMEFVRQKRAVESGTQGSS+PR
Sbjct: 121 FLKLIAKPAEHLPSAEIQLERKEAEQSGAAKETPIVTPLMEFVRQKRAVESGTQGSSVPR 180

Query: 181 KVKRGGGASSRKPESNSAKRGMEKKKYILKESVKNTNRRDKSNFILVPRREDQLATSGGI 240
           KVKRGG ASSRKPESNS KRGMEKKKYILK+SVKNTNRRDKSNFILVPRR+DQ A S GI
Sbjct: 181 KVKRGGAASSRKPESNSMKRGMEKKKYILKDSVKNTNRRDKSNFILVPRRDDQSANSSGI 240

Query: 241 GISDVGIADSGKKKILLLKGKERDISHVSDGMLQLQSVTSTGTSPASASKHNQRREASGS 300
           GISDVG AD GKKKILLLKGKERDISHVSD MLQLQS TS+G SPASASKHN RREA GS
Sbjct: 241 GISDVGAADFGKKKILLLKGKERDISHVSDDMLQLQSATSSGNSPASASKHNHRREAGGS 300

Query: 301 MIRSILLNNESRHGQTSSGAPSHQKIQILNSDNGKRLPRSINARSGSNDMSSNEPNSSGL 360
           +IRSILLNNE+RHGQ+SS A SHQKIQILNSDNGKR PR  NARSGSND+SSNEPN SG 
Sbjct: 301 VIRSILLNNEARHGQSSSVAQSHQKIQILNSDNGKRPPRPTNARSGSNDISSNEPNPSGS 360

Query: 361 EGDGKRPSDGKPSKKELHGLGSLSEKQEKRIRNKDRPDRGVWAPRSRSDASVSQLEESSV 420
           EGDGKR  D K SKKELHGLGS SEKQEKRIRNKDRPDRGVWAPRSRSDASVSQLEESSV
Sbjct: 361 EGDGKRAPDNKFSKKELHGLGSASEKQEKRIRNKDRPDRGVWAPRSRSDASVSQLEESSV 420

Query: 421 AQSSHLLSDSVEAYRGEMKEDIHVSRTGDVTTTVSGRNSSVENGSVRHGGRRGAGHVMKD 480
           +QSSHLLSDSVEAYRGEMKEDIH SRT DVTT VSGRNSSVENGSVRH GRRGAGHVMKD
Sbjct: 421 SQSSHLLSDSVEAYRGEMKEDIHGSRTADVTTIVSGRNSSVENGSVRHVGRRGAGHVMKD 480

Query: 481 DGSLNPNEGKPSKRGVASGHEKQVWVQKSSSGS 514
           DGSLNPNEGKPSKRGVA GHEKQVWVQKSSSGS
Sbjct: 481 DGSLNPNEGKPSKRGVAGGHEKQVWVQKSSSGS 513

BLAST of CmaCh09G012310 vs. ExPASy TrEMBL
Match: A0A6J1D8M3 (regulator of nonsense transcripts UPF3 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111018259 PE=3 SV=1)

HSP 1 Score: 873.6 bits (2256), Expect = 4.0e-250
Identity = 457/513 (89.08%), Postives = 480/513 (93.57%), Query Frame = 0

Query: 1   MKDPLERTKVVIRHLPPSLPHSDLFHNIDDRFAGRYNWCYYRPGKTSQKDQRYARAYIDF 60
           MKDPLERTKVVIRHLPPSLPHSDLFH+IDDRFAGRYNWCYYRPGKTSQKDQRY+RAYIDF
Sbjct: 1   MKDPLERTKVVIRHLPPSLPHSDLFHHIDDRFAGRYNWCYYRPGKTSQKDQRYSRAYIDF 60

Query: 61  TRPEDVFEFAEFFDGHVFVNEKGAQFKAVVEYAPSQRVPRSSTKKDGREGTIYKDPDYLE 120
           TRPEDVFEFAEFFDGHVFVNEKG Q+KAV+EYAPSQRVPRSSTKKDGR+GTIYKDPDYLE
Sbjct: 61  TRPEDVFEFAEFFDGHVFVNEKGTQYKAVIEYAPSQRVPRSSTKKDGRDGTIYKDPDYLE 120

Query: 121 FLKLIAKPAEHLPSAEIQLERKEAEQSGAAKETPIVTPLMEFVRQKRAVESGTQGSSLPR 180
           FLKLIAKPAEHLPSAEIQLERKEAEQSG AKETPIVTPLMEFVRQKRAVESGTQGSS+ R
Sbjct: 121 FLKLIAKPAEHLPSAEIQLERKEAEQSGVAKETPIVTPLMEFVRQKRAVESGTQGSSVSR 180

Query: 181 KVKRGGGASSRKPESNSAKRGMEKKKYILKESVKNTNRRDKSNFILVPRREDQLATSGGI 240
           KVKRGGGASSRKPESNS KRGMEKKKYILK+SVKNTNRRDKSNFILVPRR+DQ ATS GI
Sbjct: 181 KVKRGGGASSRKPESNSMKRGMEKKKYILKDSVKNTNRRDKSNFILVPRRDDQPATSSGI 240

Query: 241 GISDVGIADSGKKKILLLKGKERDISHVSDGMLQLQSVTSTGTSPASASKHNQRREASGS 300
           GISDV  ADSGKKKILLLKGKERDISHV+DGML LQS +S+G SP +A+K NQRREASGS
Sbjct: 241 GISDVTTADSGKKKILLLKGKERDISHVADGMLHLQSASSSGNSPTTATKQNQRREASGS 300

Query: 301 MIRSILLNNESRHGQTSSGAPSHQKIQILNSDNGKRLPRSINARSGSNDMSSNEPNSSGL 360
           +IRSILLNNE RHGQ SS + SHQKIQILNSDNGKR PR INARSGSNDMS+NEPN SG 
Sbjct: 301 LIRSILLNNEPRHGQGSSVSQSHQKIQILNSDNGKRPPRPINARSGSNDMSNNEPNPSGS 360

Query: 361 EGDGKRPSDGKPSKKELHGLGSLSEKQEKRIRNKDRPDRGVWAPRSRSDASVSQLEESSV 420
           EGDGKR  + K SKKELHGLGS SEKQEKRIRNKDRPDRGVWAPRSRSDA VSQL+ESS+
Sbjct: 361 EGDGKRAPESKFSKKELHGLGSASEKQEKRIRNKDRPDRGVWAPRSRSDALVSQLDESSI 420

Query: 421 AQSSHLLSDSVEAYRGEMKEDIHVSRTGDVTTTVSGRNSSVENGSVRHGGRRGAGHVMKD 480
           +QSSHLLSDSVEA+R EMK+DIH SRTGDVTTTVSGR SSVENGSVRH GRRGAGHVMKD
Sbjct: 421 SQSSHLLSDSVEAFRVEMKDDIHGSRTGDVTTTVSGRGSSVENGSVRHVGRRGAGHVMKD 480

Query: 481 DGSLNPNEGKPSKRGVASGHEKQVWVQKSSSGS 514
           DGSLNPNEGK SKRG+A GHEKQVWVQKSSSGS
Sbjct: 481 DGSLNPNEGKSSKRGLAGGHEKQVWVQKSSSGS 513

BLAST of CmaCh09G012310 vs. NCBI nr
Match: XP_022973974.1 (regulator of nonsense transcripts UPF3-like [Cucurbita maxima])

HSP 1 Score: 981.5 bits (2536), Expect = 2.8e-282
Identity = 513/513 (100.00%), Postives = 513/513 (100.00%), Query Frame = 0

Query: 1   MKDPLERTKVVIRHLPPSLPHSDLFHNIDDRFAGRYNWCYYRPGKTSQKDQRYARAYIDF 60
           MKDPLERTKVVIRHLPPSLPHSDLFHNIDDRFAGRYNWCYYRPGKTSQKDQRYARAYIDF
Sbjct: 1   MKDPLERTKVVIRHLPPSLPHSDLFHNIDDRFAGRYNWCYYRPGKTSQKDQRYARAYIDF 60

Query: 61  TRPEDVFEFAEFFDGHVFVNEKGAQFKAVVEYAPSQRVPRSSTKKDGREGTIYKDPDYLE 120
           TRPEDVFEFAEFFDGHVFVNEKGAQFKAVVEYAPSQRVPRSSTKKDGREGTIYKDPDYLE
Sbjct: 61  TRPEDVFEFAEFFDGHVFVNEKGAQFKAVVEYAPSQRVPRSSTKKDGREGTIYKDPDYLE 120

Query: 121 FLKLIAKPAEHLPSAEIQLERKEAEQSGAAKETPIVTPLMEFVRQKRAVESGTQGSSLPR 180
           FLKLIAKPAEHLPSAEIQLERKEAEQSGAAKETPIVTPLMEFVRQKRAVESGTQGSSLPR
Sbjct: 121 FLKLIAKPAEHLPSAEIQLERKEAEQSGAAKETPIVTPLMEFVRQKRAVESGTQGSSLPR 180

Query: 181 KVKRGGGASSRKPESNSAKRGMEKKKYILKESVKNTNRRDKSNFILVPRREDQLATSGGI 240
           KVKRGGGASSRKPESNSAKRGMEKKKYILKESVKNTNRRDKSNFILVPRREDQLATSGGI
Sbjct: 181 KVKRGGGASSRKPESNSAKRGMEKKKYILKESVKNTNRRDKSNFILVPRREDQLATSGGI 240

Query: 241 GISDVGIADSGKKKILLLKGKERDISHVSDGMLQLQSVTSTGTSPASASKHNQRREASGS 300
           GISDVGIADSGKKKILLLKGKERDISHVSDGMLQLQSVTSTGTSPASASKHNQRREASGS
Sbjct: 241 GISDVGIADSGKKKILLLKGKERDISHVSDGMLQLQSVTSTGTSPASASKHNQRREASGS 300

Query: 301 MIRSILLNNESRHGQTSSGAPSHQKIQILNSDNGKRLPRSINARSGSNDMSSNEPNSSGL 360
           MIRSILLNNESRHGQTSSGAPSHQKIQILNSDNGKRLPRSINARSGSNDMSSNEPNSSGL
Sbjct: 301 MIRSILLNNESRHGQTSSGAPSHQKIQILNSDNGKRLPRSINARSGSNDMSSNEPNSSGL 360

Query: 361 EGDGKRPSDGKPSKKELHGLGSLSEKQEKRIRNKDRPDRGVWAPRSRSDASVSQLEESSV 420
           EGDGKRPSDGKPSKKELHGLGSLSEKQEKRIRNKDRPDRGVWAPRSRSDASVSQLEESSV
Sbjct: 361 EGDGKRPSDGKPSKKELHGLGSLSEKQEKRIRNKDRPDRGVWAPRSRSDASVSQLEESSV 420

Query: 421 AQSSHLLSDSVEAYRGEMKEDIHVSRTGDVTTTVSGRNSSVENGSVRHGGRRGAGHVMKD 480
           AQSSHLLSDSVEAYRGEMKEDIHVSRTGDVTTTVSGRNSSVENGSVRHGGRRGAGHVMKD
Sbjct: 421 AQSSHLLSDSVEAYRGEMKEDIHVSRTGDVTTTVSGRNSSVENGSVRHGGRRGAGHVMKD 480

Query: 481 DGSLNPNEGKPSKRGVASGHEKQVWVQKSSSGS 514
           DGSLNPNEGKPSKRGVASGHEKQVWVQKSSSGS
Sbjct: 481 DGSLNPNEGKPSKRGVASGHEKQVWVQKSSSGS 513

BLAST of CmaCh09G012310 vs. NCBI nr
Match: XP_023535567.1 (regulator of nonsense transcripts UPF3-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 971.8 bits (2511), Expect = 2.2e-279
Identity = 507/513 (98.83%), Postives = 509/513 (99.22%), Query Frame = 0

Query: 1   MKDPLERTKVVIRHLPPSLPHSDLFHNIDDRFAGRYNWCYYRPGKTSQKDQRYARAYIDF 60
           MKDPLERTKVVIRHLPPSLPHSDLFHNIDDRFAGRYNWCYYRPGKTSQKDQRYARAYIDF
Sbjct: 1   MKDPLERTKVVIRHLPPSLPHSDLFHNIDDRFAGRYNWCYYRPGKTSQKDQRYARAYIDF 60

Query: 61  TRPEDVFEFAEFFDGHVFVNEKGAQFKAVVEYAPSQRVPRSSTKKDGREGTIYKDPDYLE 120
           TRPEDVFEFAEFFDGHVFVNEKGAQFKAVVEYAPSQRVPRSSTKKDGREGTIYKDPDYLE
Sbjct: 61  TRPEDVFEFAEFFDGHVFVNEKGAQFKAVVEYAPSQRVPRSSTKKDGREGTIYKDPDYLE 120

Query: 121 FLKLIAKPAEHLPSAEIQLERKEAEQSGAAKETPIVTPLMEFVRQKRAVESGTQGSSLPR 180
           FLKLIAKPAEHLPSAEIQLERKEAEQSGAAKETPIVTPLMEFVRQKRAVESGTQGSS+PR
Sbjct: 121 FLKLIAKPAEHLPSAEIQLERKEAEQSGAAKETPIVTPLMEFVRQKRAVESGTQGSSVPR 180

Query: 181 KVKRGGGASSRKPESNSAKRGMEKKKYILKESVKNTNRRDKSNFILVPRREDQLATSGGI 240
           KVKRGGGASSRKPESNS KRGMEKKKYILKESVKNTNRRDKSNFILVPRREDQLATSGGI
Sbjct: 181 KVKRGGGASSRKPESNSVKRGMEKKKYILKESVKNTNRRDKSNFILVPRREDQLATSGGI 240

Query: 241 GISDVGIADSGKKKILLLKGKERDISHVSDGMLQLQSVTSTGTSPASASKHNQRREASGS 300
           GISDVG ADSGKKKILLLKGKERDISHVSDGMLQLQS TS+GTSPASASKHNQRREASGS
Sbjct: 241 GISDVGTADSGKKKILLLKGKERDISHVSDGMLQLQSATSSGTSPASASKHNQRREASGS 300

Query: 301 MIRSILLNNESRHGQTSSGAPSHQKIQILNSDNGKRLPRSINARSGSNDMSSNEPNSSGL 360
           MIRSILLNNESRHGQTSSGAPSHQKIQILNSDNGKRLPRSINARSGSNDMSSNEPN SGL
Sbjct: 301 MIRSILLNNESRHGQTSSGAPSHQKIQILNSDNGKRLPRSINARSGSNDMSSNEPNPSGL 360

Query: 361 EGDGKRPSDGKPSKKELHGLGSLSEKQEKRIRNKDRPDRGVWAPRSRSDASVSQLEESSV 420
           EGDGKRPSDGKPSKKELHGLGSLSEKQEKRIRNKDRPDRGVWAPRSRSDASVSQLEESSV
Sbjct: 361 EGDGKRPSDGKPSKKELHGLGSLSEKQEKRIRNKDRPDRGVWAPRSRSDASVSQLEESSV 420

Query: 421 AQSSHLLSDSVEAYRGEMKEDIHVSRTGDVTTTVSGRNSSVENGSVRHGGRRGAGHVMKD 480
           AQSSHLLSDSVEAYRGEMKEDIHVSRTGDVTTTVSGRNSSVENGSVRHGGRRGAGHVMKD
Sbjct: 421 AQSSHLLSDSVEAYRGEMKEDIHVSRTGDVTTTVSGRNSSVENGSVRHGGRRGAGHVMKD 480

Query: 481 DGSLNPNEGKPSKRGVASGHEKQVWVQKSSSGS 514
           DGSLNPNEGKPSKRGVASGHEKQVWVQKSSSGS
Sbjct: 481 DGSLNPNEGKPSKRGVASGHEKQVWVQKSSSGS 513

BLAST of CmaCh09G012310 vs. NCBI nr
Match: XP_022925470.1 (regulator of nonsense transcripts UPF3-like [Cucurbita moschata])

HSP 1 Score: 963.8 bits (2490), Expect = 6.1e-277
Identity = 503/513 (98.05%), Postives = 506/513 (98.64%), Query Frame = 0

Query: 1   MKDPLERTKVVIRHLPPSLPHSDLFHNIDDRFAGRYNWCYYRPGKTSQKDQRYARAYIDF 60
           MKDPLERTKVVIRHLPPSLPHSDLFHNIDDRFAGRYNWCYYRPGKTSQKDQRYARAYIDF
Sbjct: 1   MKDPLERTKVVIRHLPPSLPHSDLFHNIDDRFAGRYNWCYYRPGKTSQKDQRYARAYIDF 60

Query: 61  TRPEDVFEFAEFFDGHVFVNEKGAQFKAVVEYAPSQRVPRSSTKKDGREGTIYKDPDYLE 120
           TRPEDVFEFAEFFDGHVFVNEKGAQFKAVVEYAPSQRVPRSSTKKDGREGTIYKDPDYLE
Sbjct: 61  TRPEDVFEFAEFFDGHVFVNEKGAQFKAVVEYAPSQRVPRSSTKKDGREGTIYKDPDYLE 120

Query: 121 FLKLIAKPAEHLPSAEIQLERKEAEQSGAAKETPIVTPLMEFVRQKRAVESGTQGSSLPR 180
           FLKLIAKPAEHLPSAEIQLERKEAEQS AAKETPIVTPLMEFVRQKRAVESGTQGSS+PR
Sbjct: 121 FLKLIAKPAEHLPSAEIQLERKEAEQSAAAKETPIVTPLMEFVRQKRAVESGTQGSSVPR 180

Query: 181 KVKRGGGASSRKPESNSAKRGMEKKKYILKESVKNTNRRDKSNFILVPRREDQLATSGGI 240
           KVKRGGGASSRKPESNS KRGMEKKKYILKESVKNTNRRDKSNFILVPRREDQLATSGGI
Sbjct: 181 KVKRGGGASSRKPESNSVKRGMEKKKYILKESVKNTNRRDKSNFILVPRREDQLATSGGI 240

Query: 241 GISDVGIADSGKKKILLLKGKERDISHVSDGMLQLQSVTSTGTSPASASKHNQRREASGS 300
           GISDVG ADSGKKKILLLKGKERDISHVSDGMLQLQS TS+GTSPASASKHNQRREASGS
Sbjct: 241 GISDVGTADSGKKKILLLKGKERDISHVSDGMLQLQSATSSGTSPASASKHNQRREASGS 300

Query: 301 MIRSILLNNESRHGQTSSGAPSHQKIQILNSDNGKRLPRSINARSGSNDMSSNEPNSSGL 360
           MIRSILLNNESRHGQTSSGAPSHQKIQILNSDNGKRLPRSINARSGSNDMSSNEPN SGL
Sbjct: 301 MIRSILLNNESRHGQTSSGAPSHQKIQILNSDNGKRLPRSINARSGSNDMSSNEPNPSGL 360

Query: 361 EGDGKRPSDGKPSKKELHGLGSLSEKQEKRIRNKDRPDRGVWAPRSRSDASVSQLEESSV 420
           EGDGKRPSDGKPSKKELHGLGSLSEKQEKRIRNKDRPDRGVWAPRSRSDASVSQLEESSV
Sbjct: 361 EGDGKRPSDGKPSKKELHGLGSLSEKQEKRIRNKDRPDRGVWAPRSRSDASVSQLEESSV 420

Query: 421 AQSSHLLSDSVEAYRGEMKEDIHVSRTGDVTTTVSGRNSSVENGSVRHGGRRGAGHVMKD 480
           AQSSHLLSDSVEAYRGEMKEDIHVSRTGDVTTTVSGR+SSVENGSVRHGGRRGAGHVMKD
Sbjct: 421 AQSSHLLSDSVEAYRGEMKEDIHVSRTGDVTTTVSGRSSSVENGSVRHGGRRGAGHVMKD 480

Query: 481 DGSLNPNEGKPSKRGVASGHEKQVWVQKSSSGS 514
           DGS NPNEGKPSKRGV SGHEKQVWVQKSSSGS
Sbjct: 481 DGSSNPNEGKPSKRGVVSGHEKQVWVQKSSSGS 513

BLAST of CmaCh09G012310 vs. NCBI nr
Match: KAG7025200.1 (Regulator of nonsense transcripts UPF3 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 944.9 bits (2441), Expect = 2.9e-271
Identity = 496/513 (96.69%), Postives = 498/513 (97.08%), Query Frame = 0

Query: 1   MKDPLERTKVVIRHLPPSLPHSDLFHNIDDRFAGRYNWCYYRPGKTSQKDQRYARAYIDF 60
           MKDPLERTKVVIRHLPPSLPHSDLFHNIDDRFAGRYNWCYYRPGKTSQKDQRYARAYIDF
Sbjct: 1   MKDPLERTKVVIRHLPPSLPHSDLFHNIDDRFAGRYNWCYYRPGKTSQKDQRYARAYIDF 60

Query: 61  TRPEDVFEFAEFFDGHVFVNEKGAQFKAVVEYAPSQRVPRSSTKKDGREGTIYKDPDYLE 120
           TRPEDVFEFAEFFDGHVFVNEKGAQFKAVVEYAPSQRVPRSSTKKDGREGTIYKDPDYLE
Sbjct: 61  TRPEDVFEFAEFFDGHVFVNEKGAQFKAVVEYAPSQRVPRSSTKKDGREGTIYKDPDYLE 120

Query: 121 FLKLIAKPAEHLPSAEIQLERKEAEQSGAAKETPIVTPLMEFVRQKRAVESGTQGSSLPR 180
           FLKLIAKPAEHLPSAEIQLERKEAEQSGAAKETPIVTPLMEFVRQKRAVESGTQGSS+PR
Sbjct: 121 FLKLIAKPAEHLPSAEIQLERKEAEQSGAAKETPIVTPLMEFVRQKRAVESGTQGSSVPR 180

Query: 181 KVKRGGGASSRKPESNSAKRGMEKKKYILKESVKNTNRRDKSNFILVPRREDQLATSGGI 240
           KVKRGGGASSRKPESNS         YILKESVKNTNRRDKSNFILVPRREDQLATSGGI
Sbjct: 181 KVKRGGGASSRKPESNS---------YILKESVKNTNRRDKSNFILVPRREDQLATSGGI 240

Query: 241 GISDVGIADSGKKKILLLKGKERDISHVSDGMLQLQSVTSTGTSPASASKHNQRREASGS 300
           GISDVG ADSGKKKILLLKGKERDISHVSDGMLQLQS TS+GTSPASASKHNQRREASGS
Sbjct: 241 GISDVGTADSGKKKILLLKGKERDISHVSDGMLQLQSATSSGTSPASASKHNQRREASGS 300

Query: 301 MIRSILLNNESRHGQTSSGAPSHQKIQILNSDNGKRLPRSINARSGSNDMSSNEPNSSGL 360
           MIRSILLNNESRHGQTSSGAPSHQKIQILNSDNGKRLPRSINARSGSNDMSSNEPN SGL
Sbjct: 301 MIRSILLNNESRHGQTSSGAPSHQKIQILNSDNGKRLPRSINARSGSNDMSSNEPNPSGL 360

Query: 361 EGDGKRPSDGKPSKKELHGLGSLSEKQEKRIRNKDRPDRGVWAPRSRSDASVSQLEESSV 420
           EGDGKRPSDGKPSKKELHGLGSLSEKQEKRIRNKDRPDRGVWAPRSRSDASVSQLEESSV
Sbjct: 361 EGDGKRPSDGKPSKKELHGLGSLSEKQEKRIRNKDRPDRGVWAPRSRSDASVSQLEESSV 420

Query: 421 AQSSHLLSDSVEAYRGEMKEDIHVSRTGDVTTTVSGRNSSVENGSVRHGGRRGAGHVMKD 480
           AQSSHLLSDSVEAYRGEMKEDIHVSR GDVTTTVSGRNSSVENGSVRHGGRRGAGHVMKD
Sbjct: 421 AQSSHLLSDSVEAYRGEMKEDIHVSRAGDVTTTVSGRNSSVENGSVRHGGRRGAGHVMKD 480

Query: 481 DGSLNPNEGKPSKRGVASGHEKQVWVQKSSSGS 514
           DGS NPNEGKPSKRGV SGHEKQVWVQKSSSGS
Sbjct: 481 DGSSNPNEGKPSKRGVVSGHEKQVWVQKSSSGS 504

BLAST of CmaCh09G012310 vs. NCBI nr
Match: XP_038890175.1 (regulator of nonsense transcripts UPF3 isoform X1 [Benincasa hispida] >XP_038890176.1 regulator of nonsense transcripts UPF3 isoform X1 [Benincasa hispida])

HSP 1 Score: 893.3 bits (2307), Expect = 1.0e-255
Identity = 472/513 (92.01%), Postives = 483/513 (94.15%), Query Frame = 0

Query: 1   MKDPLERTKVVIRHLPPSLPHSDLFHNIDDRFAGRYNWCYYRPGKTSQKDQRYARAYIDF 60
           MKDPLERTKVVIRHLPPSLPHSDLFH+I DRF GR+NW YYRPGKTSQKDQRYARAYIDF
Sbjct: 1   MKDPLERTKVVIRHLPPSLPHSDLFHHIHDRFGGRFNWSYYRPGKTSQKDQRYARAYIDF 60

Query: 61  TRPEDVFEFAEFFDGHVFVNEKGAQFKAVVEYAPSQRVPRSSTKKDGREGTIYKDPDYLE 120
           TRPEDVFEFAEFFDGH+FVNEKGAQ+KAVVEYAPSQRVPRSSTKKDGREGTIYKDPDYLE
Sbjct: 61  TRPEDVFEFAEFFDGHIFVNEKGAQYKAVVEYAPSQRVPRSSTKKDGREGTIYKDPDYLE 120

Query: 121 FLKLIAKPAEHLPSAEIQLERKEAEQSGAAKETPIVTPLMEFVRQKRAVESGTQGSSLPR 180
           FLKLIAKPAEHLPSAEIQLERKEAEQSGAAKE PIVTPLMEFVRQKRAVESGTQGSS+PR
Sbjct: 121 FLKLIAKPAEHLPSAEIQLERKEAEQSGAAKEAPIVTPLMEFVRQKRAVESGTQGSSVPR 180

Query: 181 KVKRGGGASSRKPESNSAKRGMEKKKYILKESVKNTNRRDKSNFILVPRREDQLATSGGI 240
           KVKRGG ASSRKPE NS KRGMEKKKYILK+SVKNTNRRDKSNFILVPRREDQ ATS GI
Sbjct: 181 KVKRGGAASSRKPELNSMKRGMEKKKYILKDSVKNTNRRDKSNFILVPRREDQSATSSGI 240

Query: 241 GISDVGIADSGKKKILLLKGKERDISHVSDGMLQLQSVTSTGTSPASASKHNQRREASGS 300
           GISDV  AD GKKKILLLKGKERDISHVSDGMLQLQS TS+ TSPASASKHNQRREASGS
Sbjct: 241 GISDVVTADFGKKKILLLKGKERDISHVSDGMLQLQSATSSETSPASASKHNQRREASGS 300

Query: 301 MIRSILLNNESRHGQTSSGAPSHQKIQILNSDNGKRLPRSINARSGSNDMSSNEPNSSGL 360
           MIRSILLNNE+RHGQ SS A SH KIQILNSDNGKR PR INARSGSNDMSSNEPN SG 
Sbjct: 301 MIRSILLNNEARHGQNSSVAQSHPKIQILNSDNGKRPPRPINARSGSNDMSSNEPNPSGS 360

Query: 361 EGDGKRPSDGKPSKKELHGLGSLSEKQEKRIRNKDRPDRGVWAPRSRSDASVSQLEESSV 420
           EGDGKR  DGK SKKELHGLGS +EKQEKRIRNKDRPDRGVWAPRSRSDASVSQLEESSV
Sbjct: 361 EGDGKRAPDGKFSKKELHGLGSGNEKQEKRIRNKDRPDRGVWAPRSRSDASVSQLEESSV 420

Query: 421 AQSSHLLSDSVEAYRGEMKEDIHVSRTGDVTTTVSGRNSSVENGSVRHGGRRGAGHVMKD 480
           +QSSHLLSDSVEAYRGEMKED+H SRTGDVTTTVSGRNSSVENGSVRH GRRGA HVMKD
Sbjct: 421 SQSSHLLSDSVEAYRGEMKEDVHGSRTGDVTTTVSGRNSSVENGSVRHVGRRGAAHVMKD 480

Query: 481 DGSLNPNEGKPSKRGVASGHEKQVWVQKSSSGS 514
           DGSLNPNEGKPSKRGVA GHEKQVWVQKSSSGS
Sbjct: 481 DGSLNPNEGKPSKRGVAGGHEKQVWVQKSSSGS 513

BLAST of CmaCh09G012310 vs. TAIR 10
Match: AT1G33980.1 (Smg-4/UPF3 family protein )

HSP 1 Score: 394.4 bits (1012), Expect = 1.4e-109
Identity = 250/530 (47.17%), Postives = 323/530 (60.94%), Query Frame = 0

Query: 1   MKDPLERTKVVIRHLPPSLPHSDLFHNIDDRFAGRYNWCYYRPGKTSQKDQRYARAYIDF 60
           MK+PL++ KVV+RHLPPSL  SDL   ID RFA RYNW  +RPGK+S K+Q+Y+RAY+ F
Sbjct: 1   MKEPLQKKKVVVRHLPPSLSQSDLLSQIDPRFADRYNWVSFRPGKSSYKNQKYSRAYVSF 60

Query: 61  TRPEDVFEFAEFFDGHVFVNEKGAQFKAVVEYAPSQRVPRSSTKKDGREGTIYKDPDYLE 120
             PEDV+EFA FF+GHVFVNEKGAQFKA+VEYAPSQRVP+ S KKD REG+I KDPDYLE
Sbjct: 61  KAPEDVYEFAAFFNGHVFVNEKGAQFKAIVEYAPSQRVPKPSDKKDPREGSISKDPDYLE 120

Query: 121 FLKLIAKPAEHLPSAEIQLERKEAEQSGAAKETPIVTPLMEFVRQKRAVESGTQG-SSLP 180
           FLK+IA+P E+LPSAEIQLER+EAEQSGA+K  PIVTPLMEF+RQKRA   G QG S + 
Sbjct: 121 FLKVIAQPVENLPSAEIQLERREAEQSGASKAAPIVTPLMEFIRQKRATVMGPQGLSDIR 180

Query: 181 RKVKRGGGASSRKPESNSAKRGMEKKKYILKESVKNTNRRDKSNFILVPRREDQLATSGG 240
           R  +R    S+ KP    +KR  EKKKY+ KES KN  R+  ++         Q  +SG 
Sbjct: 181 RGGRRTRVVSANKPSPRPSKRNSEKKKYVEKESSKNVPRKTTADVSSSKPDYRQSNSSGK 240

Query: 241 ----------IGISDVGIA---DSGKKKILLLKGKERDISHVSDGMLQLQSVTSTGTSPA 300
                     I  S  GIA   DSGKKKILLL+ K+RD         + Q + +  +  +
Sbjct: 241 ELPGNETAAIIDSSPPGIALTMDSGKKKILLLRSKDRDNPDNPPPQPE-QHIDTNLSRNS 300

Query: 301 SASKHNQRREASGSMIRSILLNNESRHGQTSSGAPSHQKIQILNSDNGKRLPRSINARSG 360
           + S+ NQ+ +  G +I+ ILL N+SR  Q+S+   S Q+++   ++N KR  R  N R+G
Sbjct: 301 TDSRQNQKSDVGGRLIKGILLRNDSRPSQSSTFVQSEQRVEPSEAENYKRPSRPANTRAG 360

Query: 361 SNDMSSNEPNSSGLEGDGKRPSDGKPSKKELHGLGSLSEKQEKRIRNKDRPDRGVWAPRS 420
                                       K+ H  G++SEKQE+R RNKDRPDR +WAP  
Sbjct: 361 ----------------------------KDYHTSGTISEKQERRTRNKDRPDRVMWAP-- 420

Query: 421 RSDASVSQLEESSVAQSSHLLSDSVEAYRGEMKEDIHVSRTGDVTTTVSGRNSSVENGSV 480
           R D S  Q   S+                GE+K+ +   R+G+V  +  G   ++ENGS 
Sbjct: 421 RRDGSEDQPLSSA-------------GNNGEVKDRMFSQRSGEVVNSSGGH--TLENGSA 480

Query: 481 RHGGRRGAGHVMKDDGSLNPNEGKPSKRGVASG---HEKQVWVQKSSSGS 514
           RH  RR  G   K++  +   EGK S+RG   G   HEKQ+W+QK SSG+
Sbjct: 481 RHSSRRVGGRNRKEEVVI--GEGKTSRRGSGGGPSSHEKQMWIQKPSSGT 482

BLAST of CmaCh09G012310 vs. TAIR 10
Match: AT1G33980.2 (Smg-4/UPF3 family protein )

HSP 1 Score: 387.9 bits (995), Expect = 1.3e-107
Identity = 249/532 (46.80%), Postives = 322/532 (60.53%), Query Frame = 0

Query: 1   MKDPLERTKVVIRHLPPSLPHSDLFHNIDDRFAGRYNWCYYRPGKT--SQKDQRYARAYI 60
           MK+PL++ KVV+RHLPPSL  SDL   ID RFA RYNW  +RPGK+    K+Q+Y+RAY+
Sbjct: 1   MKEPLQKKKVVVRHLPPSLSQSDLLSQIDPRFADRYNWVSFRPGKSRLGYKNQKYSRAYV 60

Query: 61  DFTRPEDVFEFAEFFDGHVFVNEKGAQFKAVVEYAPSQRVPRSSTKKDGREGTIYKDPDY 120
            F  PEDV+EFA FF+GHVFVNEKGAQFKA+VEYAPSQRVP+ S KKD REG+I KDPDY
Sbjct: 61  SFKAPEDVYEFAAFFNGHVFVNEKGAQFKAIVEYAPSQRVPKPSDKKDPREGSISKDPDY 120

Query: 121 LEFLKLIAKPAEHLPSAEIQLERKEAEQSGAAKETPIVTPLMEFVRQKRAVESGTQG-SS 180
           LEFLK+IA+P E+LPSAEIQLER+EAEQSGA+K  PIVTPLMEF+RQKRA   G QG S 
Sbjct: 121 LEFLKVIAQPVENLPSAEIQLERREAEQSGASKAAPIVTPLMEFIRQKRATVMGPQGLSD 180

Query: 181 LPRKVKRGGGASSRKPESNSAKRGMEKKKYILKESVKNTNRRDKSNFILVPRREDQLATS 240
           + R  +R    S+ KP    +KR  EKKKY+ KES KN  R+  ++         Q  +S
Sbjct: 181 IRRGGRRTRVVSANKPSPRPSKRNSEKKKYVEKESSKNVPRKTTADVSSSKPDYRQSNSS 240

Query: 241 GG----------IGISDVGIA---DSGKKKILLLKGKERDISHVSDGMLQLQSVTSTGTS 300
           G           I  S  GIA   DSGKKKILLL+ K+RD         + Q + +  + 
Sbjct: 241 GKELPGNETAAIIDSSPPGIALTMDSGKKKILLLRSKDRDNPDNPPPQPE-QHIDTNLSR 300

Query: 301 PASASKHNQRREASGSMIRSILLNNESRHGQTSSGAPSHQKIQILNSDNGKRLPRSINAR 360
            ++ S+ NQ+ +  G +I+ ILL N+SR  Q+S+   S Q+++   ++N KR  R  N R
Sbjct: 301 NSTDSRQNQKSDVGGRLIKGILLRNDSRPSQSSTFVQSEQRVEPSEAENYKRPSRPANTR 360

Query: 361 SGSNDMSSNEPNSSGLEGDGKRPSDGKPSKKELHGLGSLSEKQEKRIRNKDRPDRGVWAP 420
           +G                            K+ H  G++SEKQE+R RNKDRPDR +WAP
Sbjct: 361 AG----------------------------KDYHTSGTISEKQERRTRNKDRPDRVMWAP 420

Query: 421 RSRSDASVSQLEESSVAQSSHLLSDSVEAYRGEMKEDIHVSRTGDVTTTVSGRNSSVENG 480
             R D S  Q   S+                GE+K+ +   R+G+V  +  G   ++ENG
Sbjct: 421 --RRDGSEDQPLSSA-------------GNNGEVKDRMFSQRSGEVVNSSGGH--TLENG 480

Query: 481 SVRHGGRRGAGHVMKDDGSLNPNEGKPSKRGVASG---HEKQVWVQKSSSGS 514
           S RH  RR  G   K++  +   EGK S+RG   G   HEKQ+W+QK SSG+
Sbjct: 481 SARHSSRRVGGRNRKEEVVI--GEGKTSRRGSGGGPSSHEKQMWIQKPSSGT 484

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9FVW41.9e-10847.17Regulator of nonsense transcripts UPF3 OS=Arabidopsis thaliana OX=3702 GN=UPF3 P... [more]
B0S7331.4e-1832.14Regulator of nonsense transcripts 3A OS=Danio rerio OX=7955 GN=upf3a PE=1 SV=2[more]
Q9BZI76.6e-1626.50Regulator of nonsense transcripts 3B OS=Homo sapiens OX=9606 GN=UPF3B PE=1 SV=1[more]
Q9H1J14.3e-1527.64Regulator of nonsense transcripts 3A OS=Homo sapiens OX=9606 GN=UPF3A PE=1 SV=1[more]
Q102672.2e-1125.74Nonsense-mediated mRNA decay protein 3 OS=Schizosaccharomyces pombe (strain 972 ... [more]
Match NameE-valueIdentityDescription
A0A6J1IG631.4e-282100.00regulator of nonsense transcripts UPF3-like OS=Cucurbita maxima OX=3661 GN=LOC11... [more]
A0A6J1EI292.9e-27798.05regulator of nonsense transcripts UPF3-like OS=Cucurbita moschata OX=3662 GN=LOC... [more]
A0A0A0KSY51.2e-25491.62Smg4_UPF3 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G650630 P... [more]
A0A1S3CB692.7e-25491.42regulator of nonsense transcripts UPF3 isoform X1 OS=Cucumis melo OX=3656 GN=LOC... [more]
A0A6J1D8M34.0e-25089.08regulator of nonsense transcripts UPF3 isoform X1 OS=Momordica charantia OX=3673... [more]
Match NameE-valueIdentityDescription
XP_022973974.12.8e-282100.00regulator of nonsense transcripts UPF3-like [Cucurbita maxima][more]
XP_023535567.12.2e-27998.83regulator of nonsense transcripts UPF3-like [Cucurbita pepo subsp. pepo][more]
XP_022925470.16.1e-27798.05regulator of nonsense transcripts UPF3-like [Cucurbita moschata][more]
KAG7025200.12.9e-27196.69Regulator of nonsense transcripts UPF3 [Cucurbita argyrosperma subsp. argyrosper... [more]
XP_038890175.11.0e-25592.01regulator of nonsense transcripts UPF3 isoform X1 [Benincasa hispida] >XP_038890... [more]
Match NameE-valueIdentityDescription
AT1G33980.11.4e-10947.17Smg-4/UPF3 family protein [more]
AT1G33980.21.3e-10746.80Smg-4/UPF3 family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR012677Nucleotide-binding alpha-beta plait domain superfamilyGENE3D3.30.70.330coord: 2..97
e-value: 2.8E-32
score: 112.9
IPR005120UPF3 domainPFAMPF03467Smg4_UPF3coord: 6..168
e-value: 9.1E-51
score: 172.3
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 447..465
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 311..359
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 168..205
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 364..403
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 407..429
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 277..298
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 311..513
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 432..446
NoneNo IPR availablePANTHERPTHR13112:SF5REGULATOR OF NONSENSE TRANSCRIPTS UPF3coord: 1..512
NoneNo IPR availableCDDcd12455RRM_like_Smg4_UPF3coord: 9..94
e-value: 2.20949E-34
score: 122.361
IPR039722Nonsense-mediated mRNA decay protein 3PANTHERPTHR13112UPF3 REGULATOR OF NONSENSE TRANSCRIPTS-LIKE PROTEINcoord: 1..512
IPR035979RNA-binding domain superfamilySUPERFAMILY54928RNA-binding domain, RBDcoord: 8..97

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh09G012310.1CmaCh09G012310.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0000184 nuclear-transcribed mRNA catabolic process, nonsense-mediated decay
biological_process GO:0045727 positive regulation of translation
cellular_component GO:0005737 cytoplasm
cellular_component GO:0005730 nucleolus
molecular_function GO:0003729 mRNA binding
molecular_function GO:0003676 nucleic acid binding