CmaCh09G011720 (gene) Cucurbita maxima (Rimu) v1.1

BLAST of CmaCh09G011720 vs. ExPASy Swiss-Prot
Match: Q9XID0 (Putative 1-phosphatidylinositol-3-phosphate 5-kinase FAB1D OS=Arabidopsis thaliana OX=3702 GN=FAB1D PE=3 SV=1)

HSP 1 Score: 1117.8 bits (2890), Expect = 0.0e+00
Identity = 673/1513 (44.48%), Postives = 910/1513 (60.15%), Query Frame = 0

Query: 59   LSPMPSLSSCDSDCSTVANLDIKDGQEG-----TTRSSQDSIDCRQERMSQNWSEVVHSN 118
            ++P  SLSS +   S   ++D      G     ++ SSQ+ ++  +E         V   
Sbjct: 1    MTPSNSLSSSERSLSGECSVDGNSCDRGIEDECSSHSSQEDVELTKE-------VKVDRL 60

Query: 119  GQLSGRDDESVITISQEATHNGSGVRVNAEIEQSHSNWLDTDLWDPPEPEDPQDHVEGGM 178
             + S      ++ I  E +   S   V   +     +  D  +W+PPEPE+P+D V+G  
Sbjct: 61   ERKSKSMPSDILDILDEKSKENSVENVQF-LSDREDDSDDVPVWEPPEPENPEDEVDGVF 120

Query: 179  GYDDDDDDEFDDSTEWNTSSSFSRSVDEASISYRIKEQKQRAMQQIMNGKFKVFIGHLLK 238
                DDDD+  D ++WN +S      DE+S   ++ E+ +R M +  + KFK  +  L+K
Sbjct: 121  A---DDDDDCCDGSKWNKASLLGELSDESSEKRKVYEENRRVMLEEADSKFKFIVSQLIK 180

Query: 239  FVGVASSGEGSENWVDIVSSLSWEAATFLKPVVNGKAVDPEAHVKVKCIATGTRSQSQFV 298
              G   S E S  W +IV+ L WEAA+ LKP ++GK+VDP  ++KVKCIATG+   S+  
Sbjct: 181  SAGF--SIEESGYWFEIVARLCWEAASMLKPAIDGKSVDPTEYIKVKCIATGSCVDSEVF 240

Query: 299  KGMVFKKHAAHKHMPTHCKNPKLILIEGMLGEAPISRLSSFNSMDQEND----FTKHVIE 358
            KG+VFKKHAA KHM T  ++P+++L+EG+LG  PIS  SS  S++Q+N+    + K V++
Sbjct: 241  KGLVFKKHAALKHMATKYEHPRIMLVEGVLGH-PISGFSSLQSVNQDNEYLLKYVKPVVD 300

Query: 359  MIEVCTANVILVEKTTSRVIQEAMLKKGMTLVLDMKLHRLERIALCTGSPILTSETLMSQ 418
            +IE    +V+LVEK+ SR IQ+ +L KG+TLV DMKLHRL+RI+ C GSPIL+ ++L SQ
Sbjct: 301  IIEASKPDVMLVEKSVSRDIQKTILDKGVTLVFDMKLHRLQRISRCIGSPILSVDSLSSQ 360

Query: 419  NTRQCDAVYFEKIVEEHAGLLEGGKRPTKTLMFIEGCPTRLGCTILLKGAHSDELKKIKV 478
              + CD+   EKIVEEH    E  K+PTKTLMF+EGCPTRLGCTILLKG HS+ LKK+K 
Sbjct: 361  KLKHCDSFRIEKIVEEHNAAGESDKKPTKTLMFLEGCPTRLGCTILLKGCHSERLKKVKE 420

Query: 479  VVQIAVVMAFHLMLETSFLVDQRAMFATI------------------------PFGGVPA 538
            VVQ + ++A+HLMLE SFL D+  MF+TI                        P   V  
Sbjct: 421  VVQYSFILAYHLMLEASFLADRHTMFSTIFAKEATSCVVEIENFSPSPSPRESPSEAVDI 480

Query: 539  TVSSDPQSPLEPCSPNAQG------ASNGSNL-EGEPYNAAIFSGLSSISDSLKRVMGES 598
             VS+         +  A G       S+G ++   EPYN  IF+G SS+S  L       
Sbjct: 481  PVSNGFDEQTIQINGEADGEKVGTWESDGDHVFSHEPYNPVIFTGFSSLSARL------- 540

Query: 599  FFLASPCQSFSSYFGHGKDLSGLVAKSDSIPSTPPEADQF----DVEVRGSSDEENSIHE 658
                      S Y G  ++   +    D+  ST    D      +     + D++  + +
Sbjct: 541  ----------SKYLGFVQNPESVPVSVDTDVSTTSNLDSIRESEEDTAEKNEDKQPLLLD 600

Query: 659  QFVPHQSTFDEGFGFHETAPNYSEDKLPKKTPFNSQSILVLMSSRNALKGTMCEQSHFSH 718
              +P  S+ D+G        N S+ +   ++   SQSILVL+S RNAL+G MC+Q HFSH
Sbjct: 601  PELPVNSSSDDG-------DNKSQTENDIESTLESQSILVLVSKRNALRGIMCDQRHFSH 660

Query: 719  IVFYKNFDAPLGKFLQENLLNQKNVCTVCGELPEAHYYYYAHSRKQLCIQVKQLPVDKVL 778
            I FYK+FD PL KFL++ + NQ+N+C  C E PEAH YYYAH  KQL IQ+K++PV K L
Sbjct: 661  IKFYKHFDVPLEKFLRD-MFNQRNLCQTCVEFPEAHLYYYAHQNKQLTIQIKRIPVAKGL 720

Query: 779  PGETEGKLWMWSRCSKCKSKGGPSKSTKRVLISTAARGLSFGKFLELCFSDCTLPSKSSV 838
             GE +GK+WMWSRC KCK+K    KSTKRVLISTAAR LSFGKFLEL FS  T  ++SS 
Sbjct: 721  AGEAKGKIWMWSRCGKCKTKNASRKSTKRVLISTAARSLSFGKFLELSFSQQTFLNRSSS 780

Query: 839  CGHSLFGDFLYFFGLGNMVAMFRYTEVAIYTVSMPPQKLEFNNSMRQGHLITETENVYEK 898
            CGHS   DFL+FFGLG+MVAM  Y++VA YTVS+PP KLE +  ++ G L  E + V+ K
Sbjct: 781  CGHSFDSDFLHFFGLGSMVAMLSYSQVASYTVSLPPMKLESSILIKAGWLEKEFQTVFTK 840

Query: 899  GMILFTEIAQFLKKIISERKSSTVND------FSLLEEMLNEERSEFELNIQSSLTKKGN 958
            G+ LF + A FLK++ S+  +S +         S +EE+L  ER  FE NI++S  K   
Sbjct: 841  GISLFEDAAGFLKRLRSQFTNSDLRYQRARKLLSNIEELLKHERCIFEENIKNSFDKAKT 900

Query: 959  PDFPFHKYLSLNRLLWELLLESCIWDKRLQLLTSLGVTINSGTSENVEPEPVRHEGLESI 1018
             D   H+ L LNR+ WELLL++ IW+ RLQ L      ++     + +   +  +GL+++
Sbjct: 901  IDDVSHRLLRLNRMRWELLLQALIWNYRLQSL-----VLSDRLLPSSDETKIYEQGLKTV 960

Query: 1019 AQTDDRNVQQDVSVDENALHS--KEIAVEHLD------GESDGDELGLPSATEVTEIPIM 1078
            ++      + D  V ++  +       VEH D         D D++      E  E   +
Sbjct: 961  SEAGMTRYENDNKVSDSGSNGGIDTPLVEHKDIPIAGASVGDNDQMAESYVPEDNESQTL 1020

Query: 1079 DDLSPKQLS--------RQGSLLNGFNRRHSDDEDPQAGRVLSSGDIPSSTGN--LTSDK 1138
               SP   S             ++  N + +D   P  G  L   ++ +S G   L  D+
Sbjct: 1021 CSSSPDTTSPINNHFDTHLAVNVHSTNGQEADKSIPVTGESLDD-EVSTSNGPHILGWDE 1080

Query: 1139 LFWTPFSEIQQMRMRDIQRSYFPELKSISNYTPKLLPTAHEFINKEGQKLHIFLSNEKFI 1198
             FW PF E++  R+ DI++ Y  + + ++N+T + L T ++ I +E  +L I L ++ FI
Sbjct: 1081 WFWLPFEELRSKRIVDIEKEYLLKFEYVNNFTQENLQTVNQIITEESSRLRISLRDDDFI 1140

Query: 1199 VSDYEGELSSIVACVLALLKDLSLETDLHNEDSKGEGLNKVPSS----GSSDSDSSVTSE 1258
            VSDYE ELSS++AC LA          L+NE+SK      +  S      ++ DS  T  
Sbjct: 1141 VSDYEDELSSLIACALA---------HLNNEESKKPLSRCIHGSLQGFLDNNQDSKQTDR 1200

Query: 1259 EHQFSSFDRLNLLDSLLPK----TFKRAEHEGVIKSLAKGKYLVNCPYFNQFRDLRRRCC 1318
            +    S +  N L++L P     TF      G +KS+ K KY +   Y + FRDLR+RCC
Sbjct: 1201 DVSRFSSESTNRLETLPPPEVLVTF------GSVKSVGKPKYSIVSLYADDFRDLRKRCC 1260

Query: 1319 PSELHYIASLSRCINWNAKGGKSKSFFAKTLDDRFIIKEIKRTEYESFMKFAPDYFKYIN 1378
             SEL YIASLSRC  W+AKGGKSKS FAKTLDDRFI+KEIK+TEYESF+ FA +YFKY+ 
Sbjct: 1261 SSELDYIASLSRCKPWDAKGGKSKSVFAKTLDDRFIVKEIKKTEYESFVTFATEYFKYMK 1320

Query: 1379 ESFDMGNQTCLAKVLGIYQVTVREPK-SGKEVRHELMVMENLSFGRNIIRQYDLKGALHA 1438
            +S+D+GNQTCLAKVLGI+QVTVR+PK  GKE+RH+LMVMENLSF R + RQYDLKGALHA
Sbjct: 1321 DSYDLGNQTCLAKVLGIHQVTVRQPKGGGKEIRHDLMVMENLSFSRKVTRQYDLKGALHA 1380

Query: 1439 RYN-PATNDFGEVLLDQNFVNDMNSSPLYVNNQAKRRLQRAIWNDTAFLNSMNVMDYSLL 1493
            R+   + N   +VLLDQNFVNDMN SPLYV+  +K+ LQRA++NDT+FL S+NVMDYSLL
Sbjct: 1381 RFTATSANGEDDVLLDQNFVNDMNKSPLYVSKTSKQNLQRAVYNDTSFLTSINVMDYSLL 1440

BLAST of CmaCh09G011720 vs. ExPASy Swiss-Prot
Match: Q9LUM0 (1-phosphatidylinositol-3-phosphate 5-kinase FAB1B OS=Arabidopsis thaliana OX=3702 GN=FAB1B PE=2 SV=1)

HSP 1 Score: 770.4 bits (1988), Expect = 3.8e-221
Identity = 545/1476 (36.92%), Postives = 778/1476 (52.71%), Query Frame = 0

Query: 156  LWDPPEPEDPQDHVEGGMGYDDDDDDEFDDSTEW---NTSSSFSRSVDEASISYRIKEQK 215
            LW PPEPE+ +D  E  +   D++D+E D S EW     S+SF     E     R  E+ 
Sbjct: 318  LWVPPEPENEEDERESAL--FDEEDNEGDASGEWGYLRPSTSFGSG--EYRGEDRTTEEH 377

Query: 216  QRAMQQIMNGKFKVFIGHLLKFVGVA-SSGEGSENWVDIVSSLSWEAATFLKP-VVNGKA 275
            ++AM+ +++G F+  +  LL+   ++ S  EG E+W++I++SLSWEAA  LKP +     
Sbjct: 378  KKAMKNVVDGHFRALLAQLLQVENISVSDEEGKESWLEIITSLSWEAANLLKPDMSKSGG 437

Query: 276  VDPEAHVKVKCIATGTRSQSQFVKGMVFKKHAAHKHMPTHCKNPKLILIEGMLGEAPIS- 335
            +DP  +VKVKC+A+G R  S  VKG+V KK+  ++ M T  +  +L+++ G L    +S 
Sbjct: 438  MDPGGYVKVKCLASGFRHDSMVVKGVVCKKNVVNRRMSTKIEKARLLILGGGLEYQRVSN 497

Query: 336  RLSSFNS-MDQENDFTKHVIEMIEVCTANVILVEKTTSRVIQEAMLKKGMTLVLDMKLHR 395
            +LSSF++ + QE D  K  +  I     N++LVEK+ SR  QE +L K ++LVL++K   
Sbjct: 498  QLSSFDTLLQQEKDHLKMAVAKIHAERPNILLVEKSVSRFAQEYLLAKDISLVLNIKRPL 557

Query: 396  LERIALCTGSPILTS-ETLMSQNTRQCDAVYFEKIVEEHAGLLEGGKRPTKTLMFIEGCP 455
            L+RIA CTG+ I+ S + L SQ    C+    ++  EEH    + GK+  KTLM+ E CP
Sbjct: 558  LDRIARCTGAQIIPSVDHLSSQKLGYCENFRVDRYPEEHGSTGQVGKKVVKTLMYFEHCP 617

Query: 456  TRLGCTILLKGAHSDELKKIKVVVQIAVVMAFHLMLETSFLVDQRA---------MFATI 515
              LG TILL+GA+ DELKK+K VVQ  V  A+HL LETSFL D+ A         +   +
Sbjct: 618  KPLGFTILLRGANEDELKKVKHVVQYGVFAAYHLALETSFLADEGASPELPLNSPITVAL 677

Query: 516  P---------FGGVPA-TVSSDPQSP--LEPCSPNAQGASNGSNLEGEPYNAAIFSGLSS 575
            P            VP  TVS+  +SP  L    P    +   S L     N +I   +  
Sbjct: 678  PDKSTSIERSISTVPGFTVSTYEKSPTMLSCAEPQRANSVPVSELLSTTTNLSIQKDIPP 737

Query: 576  I---SDSLKRVMGESFFLASPCQSFS----SYFGHGKDLSGLVAKSDSIPSTPPEADQFD 635
            I   S    R +  SF  +    S +           DLSG      S+P   P AD+ +
Sbjct: 738  IPYGSGWQAREINPSFVFSRHNISLNLPDRVIESRNSDLSG-----RSVPVDTP-ADKSN 797

Query: 636  VEVRGSSDEENSIH---EQFVPHQSTF-------DEGFGFHETAPNYSEDKLPKKT---- 695
              V       NS+H   + FV   S         ++  G   T      ++ PK+T    
Sbjct: 798  PIVVADETTNNSLHLSGQGFVRKSSQIGTSIMVENQDNGSELTIAQQQNNEKPKETQSQK 857

Query: 696  ------PFNSQSILVLMSSRNALKGTMCEQSHFSHIVFYKNFDAPLGKFLQENLLNQKNV 755
                  P + QSILV +SSR+  KGT+CE+SH   I +Y +FD PLG+FL+++L +Q   
Sbjct: 858  EEFPPSPSDHQSILVSLSSRSVWKGTVCERSHLFRIKYYGSFDKPLGRFLRDHLFDQSYR 917

Query: 756  CTVCGELPEAHYYYYAHSRKQLCIQVKQLPVDKVLPGETEGKLWMWSRCSKCKSKGGPSK 815
            C  C    EAH + Y H +  L I VK+L  D +LPGE EGK+WMW RC +C    G   
Sbjct: 918  CRSCEMPSEAHVHCYTHRQGSLTISVKKLQ-DYLLPGEKEGKIWMWHRCLRCPRLNGFPP 977

Query: 816  STKRVLISTAARGLSFGKFLELCFSDCTLPSKSSVCGHSLFGDFLYFFGLGNMVAMFRYT 875
            +T RV++S AA GLSFGKFLEL FS+    S+ + CGHSL  D L F+G GNMVA FRY 
Sbjct: 978  ATLRVVMSDAAWGLSFGKFLELSFSNHAAASRVACCGHSLHRDCLRFYGFGNMVACFRYA 1037

Query: 876  EVAIYTVSMPPQKLEFNNSMRQGHLITETENVYEKGMILFTEIAQFLKKIISERKSSTVN 935
             + +++V +PP  L FN    Q  +  ET+ V E+  +LF+E+   + +I  +     + 
Sbjct: 1038 TIDVHSVYLPPSILSFNYE-NQDWIQRETDEVIERAELLFSEVLNAISQIAEKGFRRRIG 1097

Query: 936  DFSLLEEMLNEERSEFELNIQSSLTKKGNPDFPFHKYLSLNRLLWELLLESCIWDKRLQL 995
            +   LEE+L +E++EFE N+Q  L ++ N   P    L L R+  +LL +S +WD RL  
Sbjct: 1098 E---LEEVLQKEKAEFEENMQKILHREVNEGQPLVDILELYRIHRQLLFQSYMWDHRLIN 1157

Query: 996  LTSLGVTINSGTSENVEPE----------PVRHEGLESIAQTDDRNVQQ--DVSVDENAL 1055
             ++L    NS  ++  E E          P  + G  S+    + N+    D + D  +L
Sbjct: 1158 ASTLHKLENSDDTKREENEKPPLAKSQTLPEMNAGTNSLLTGSEVNLNPDGDSTGDTGSL 1217

Query: 1056 HSKEIAVEH----LDGESDGDELGLPSATEVTEIPIMDDLSPKQLSRQGSLL-------- 1115
            ++ +   +        + DG E+        T  P+ + +  ++    G ++        
Sbjct: 1218 NNVQKEADTNSDLYQEKDDGGEVSPSKTLPDTSYPLENKVDVRRTQSDGQIVMKNLSATL 1277

Query: 1116 --NGFNRRHSDDEDPQAGRV---------------LSSG--------------------- 1175
                   R +  E P   +V               +S G                     
Sbjct: 1278 DAAWIGERQTSVEIPTNNKVSLPPSTMSNSSTFPPISEGLMPIDLPEQQNEFKVAYPVSP 1337

Query: 1176 DIPSSTGNLTSDKLFW--TPFSEIQQMRMRDIQRSYF---PELKSISNYTPKLLPTAHEF 1235
             +PS     + D + W   PF        R I +++     +L +   ++P  + +  E 
Sbjct: 1338 ALPSKNYENSEDSVSWLSVPFLNF----YRSINKNFLLSSQKLDTFGEHSPIYISSFREA 1397

Query: 1236 INKEGQKLHIFLSNEKFIVSDYEGELSSIVACVLALLKDLSLETDLHNEDSKGEGLNKVP 1295
              + G +L + +     +V  Y+ E +S++A  L     +S E       ++GE L   P
Sbjct: 1398 ELQGGPRLLLPVGLNDIVVPVYDDEPTSMIAYAL-----MSPEYQ-RQTSAEGESLVSYP 1457

Query: 1296 SS------------GSSDSDSSVTSEEHQFSSFDRLNLLDSLLPKTFKRAEHE----GVI 1355
            S               S S+ SV       SS    +LLD   P ++ +A H     G  
Sbjct: 1458 SELNIPRPVDDTIFDPSRSNGSVDESILSISSSRSTSLLD---PLSYTKALHARVSYGED 1517

Query: 1356 KSLAKGKYLVNCPYFNQFRDLRRRCCPSELHYIASLSRCINWNAKGGKSKSFFAKTLDDR 1415
             +L K KY V C Y  +F  LR  C PSEL YI SLSRC  W A+GGKS  FFAKTLDDR
Sbjct: 1518 GTLGKVKYTVTCYYAKRFEALRGICLPSELEYIRSLSRCKKWGAQGGKSNVFFAKTLDDR 1577

Query: 1416 FIIKEIKRTEYESFMKFAPDYFKYINESFDMGNQTCLAKVLGIYQVTVREPKSGKEVRHE 1475
            FIIK++ +TE ESF+KFAP YFKY++ES    + TCLAK+LGIYQV  ++ KSGKE + +
Sbjct: 1578 FIIKQVTKTELESFIKFAPAYFKYLSESISTKSPTCLAKILGIYQVATKQLKSGKETKMD 1637

Query: 1476 LMVMENLSFGRNIIRQYDLKGALHARYNPATNDFGEVLLDQNFVNDMNSSPLYVNNQAKR 1490
            +++MENL FGR + R YDLKG+  ARYNP ++   +VLLDQN +  M +SP++V N+AKR
Sbjct: 1638 VLIMENLLFGRTVKRLYDLKGSSRARYNPDSSGSNKVLLDQNLIEAMPTSPIFVGNKAKR 1697

BLAST of CmaCh09G011720 vs. ExPASy Swiss-Prot
Match: Q0WUR5 (1-phosphatidylinositol-3-phosphate 5-kinase FAB1A OS=Arabidopsis thaliana OX=3702 GN=FAB1A PE=2 SV=1)

HSP 1 Score: 740.7 bits (1911), Expect = 3.2e-212
Identity = 520/1435 (36.24%), Postives = 753/1435 (52.47%), Query Frame = 0

Query: 156  LWDPPEPEDPQDHVEGGMGYDDDDDDEFDDSTEWNTSSSFSRSVDEASISYRIKEQKQRA 215
            LW PPEPE+ +D  E  +  DD D+ +  D      S+SF+         +  K++   A
Sbjct: 311  LWLPPEPENEEDEREAVLSDDDGDEGDRGDWGYLRPSNSFNEK------DFHSKDKSSGA 370

Query: 216  MQQIMNGKFKVFIGHLLKFVGVASSGEG-SENWVDIVSSLSWEAATFLKP-VVNGKAVDP 275
            M+ ++ G F+  +  LL+   +    EG  E W+DI++SLSWEAAT LKP       +DP
Sbjct: 371  MKNVVEGHFRALVAQLLEVDNLPMVNEGDEEGWLDIITSLSWEAATLLKPDTSKSGGMDP 430

Query: 276  EAHVKVKCIATGTRSQSQFVKGMVFKKHAAHKHMPTHCKNPKLILIEGMLGEAPIS-RLS 335
              +VKVKCI  G RS+S  VKG+V KK+ AH+ M +  + P+L+++ G L    IS +LS
Sbjct: 431  GGYVKVKCIPCGRRSESMVVKGVVCKKNVAHRRMTSKIEKPRLLILGGALEYQRISNQLS 490

Query: 336  SFNS-MDQENDFTKHVIEMIEVCTANVILVEKTTSRVIQEAMLKKGMTLVLDMKLHRLER 395
            SF++ + QE D  K  +  I+    +++LVEK+ SR  QE +L K ++LVL++K   LER
Sbjct: 491  SFDTLLQQEMDHLKMAVAKIDSHNPDILLVEKSVSRFAQEYLLAKDISLVLNIKRSLLER 550

Query: 396  IALCTGSPILTS-ETLMSQNTRQCDAVYFEKIVEEHAGLLEGGKRPTKTLMFIEGCPTRL 455
            I+ CTG+ I+ S + L S     CD  + EK VE H    +  K+  KTLMF +GCP  L
Sbjct: 551  ISRCTGAQIVPSIDQLTSPKLGYCDLFHVEKFVETHVSPCQVAKKMAKTLMFFDGCPKPL 610

Query: 456  GCTILLKGAHSDELKKIKVVVQIAVVMAFHLMLETSFLVDQRAMFATIPFGGVPATVSSD 515
            GCTILLKGAH DELKK+K V+Q  V  A+HL LETSFL D+ A    +P    P TV+  
Sbjct: 611  GCTILLKGAHEDELKKVKHVIQYGVFAAYHLALETSFLADEGASIHELPL-QTPITVALP 670

Query: 516  PQ--------SPLEPCSPNAQGASNGSNLEGEPYNA---AIFSGLSSISDSLKRVMGESF 575
             +        S +   + ++   S  + L GEP+ A      +  SS +    ++ G   
Sbjct: 671  DKPSMVNRSISTIPGFTVSSAEKSPTTELRGEPHKANGDLTGNFTSSKTHFQGKLDGNDR 730

Query: 576  FLASPCQSFSSYFGHGKDLSGLVAKSDS-IPSTPPEADQFDVEVRGSSDEENSI----HE 635
               S     +    + K    + +K D  +P+       F VE      ++ S+     E
Sbjct: 731  IDPSERLLHNLDTVYCKPPETITSKDDGLVPTLESRQLSFHVEEPSVQKDQWSVLSGATE 790

Query: 636  QFVPHQSTFD------EGFGFHETAPNYSEDKLPKKTPFNSQSILVLMSSRNALKGTMCE 695
            Q      T D      + F   E   +   D  P  +  + QSILV +S+R   KG++CE
Sbjct: 791  QVTDGGYTNDSAVIGNQNFNRQEQMESSKGDFHPSAS--DHQSILVSLSTRCVWKGSVCE 850

Query: 696  QSHFSHIVFYKNFDAPLGKFLQENLLNQKNVCTVCGELPEAHYYYYAHSRKQLCIQVKQL 755
            ++H   I +Y +FD PLG+FL++NL +Q   C  C    EAH + Y H +  L I VK+L
Sbjct: 851  RAHLLRIKYYGSFDKPLGRFLRDNLFDQDQCCPSCTMPAEAHIHCYTHRQGSLTISVKKL 910

Query: 756  PVDKVLPGETEGKLWMWSRCSKCKSKGGPSKSTKRVLISTAARGLSFGKFLELCFSDCTL 815
            P  ++LPG+ EGK+WMW RC KC    G   +T+R+++S AA GLSFGKFLEL FS+   
Sbjct: 911  P--ELLPGQREGKIWMWHRCLKCPRINGFPPATRRIVMSDAAWGLSFGKFLELSFSNHAA 970

Query: 816  PSKSSVCGHSLFGDFLYFFGLGNMVAMFRYTEVAIYTVSMPPQKLEFNNSMRQGHLITET 875
             S+ + CGHSL  D L F+G G MVA FRY  + IY V++PP KL FN    Q  L  E+
Sbjct: 971  ASRVANCGHSLHRDCLRFYGFGRMVACFRYASINIYAVTLPPAKLYFNYE-NQEWLQKES 1030

Query: 876  ENVYEKGMILFTEIAQFLKKIISE-----RKSSTVNDFSL-LEE---MLNEERSEFELNI 935
            + V +K  +LF E+ + L +I ++      K ST N   L LEE   +L + + E++ ++
Sbjct: 1031 KEVIKKAEVLFNEVQEALSQISAKTMGAGSKGSTPNKIKLSLEELAGLLEQRKKEYKDSL 1090

Query: 936  QSSL--TKKGNPDFPFHKYLSLNRLLWELLLESCIWDKRLQLLTSLGVTINSGTSENVEP 995
            Q  L   K G P       L +N+L   ++ +S  WD+ L    ++         +N  P
Sbjct: 1091 QQMLNVVKDGQPTIDI---LLINKLRRLIIFDSYAWDECLAGAANMVRNNYLEAPKNSAP 1150

Query: 996  EPV-RHEGLESIAQTDDRNVQQDVSVDENAL------------------HSKEIAVEHLD 1055
            + + R+  LE ++    +++   V++  ++L                   S + A+    
Sbjct: 1151 KVMGRNVSLEKLSDEKVKSIPTHVAICNDSLLQDADYETCLNQGKSFADTSGKFAIPEDV 1210

Query: 1056 G---------ESDGDELGLPSATEVTEI-------------PIMDDLSPKQLSRQGSLLN 1115
            G         E D  E G  +  E +++              + D L    +  Q +  N
Sbjct: 1211 GSDRPPDCRMEFDPSEGGKDNFVESSQVVKPAHTESQFQATDLSDTLDAAWIGEQTTSEN 1270

Query: 1116 GFNR---RHSDDEDPQAG--RVLSSGDIPSSTGNLTSDKLFWTPFSEIQQMRMRDIQRSY 1175
            G  R   R +     Q    R+L S    +  G  T+D+   T            + ++Y
Sbjct: 1271 GIFRPPSRAASTNGTQIPDLRLLGSESELNFKGGPTNDE-HTTQVQLPSPSFYYSLNKNY 1330

Query: 1176 FPELKS--ISNYTPKLLPTAHEFINKEGQKLHIFLSNEKFIVSDYEGELSSIVACVLALL 1235
                +   ++   P  + +  E   + G +L + L     ++  Y+ E +SI+A  L   
Sbjct: 1331 SLNSRKHIMAEDRPVYVSSYRELEWRSGARLLLPLGCNDLVLPVYDDEPTSIIAYALT-- 1390

Query: 1236 KDLSLETDLHNEDSKGEGLNKVPSSGSSDSDSSVTSEEHQFSSFDRLNLLDSLLPKTFKR 1295
                 +  +   D   + L+   S    DS + ++       S D    L S   +  + 
Sbjct: 1391 -SSEYKAQMSGSDKSRDRLDSGGSFSLFDSVNLLSLNSLSDLSVDMSRSLSSADEQVSQL 1450

Query: 1296 AEHEGVIKSL------------AKGKYLVNCPYFNQFRDLRRRCCPSELHYIASLSRCIN 1355
                  +K L             K KY V C Y  +F  LR  CCPSE  +I SL RC  
Sbjct: 1451 LHSSLYLKDLHARISFTDEGPPGKVKYSVTCYYAKEFEALRMICCPSETDFIRSLGRCRK 1510

Query: 1356 WNAKGGKSKSFFAKTLDDRFIIKEIKRTEYESFMKFAPDYFKYINESFDMGNQTCLAKVL 1415
            W A+GGKS  FFAK+LDDRFIIK++ +TE ESF+KF P YFKY+ ES    + T LAK+L
Sbjct: 1511 WGAQGGKSNVFFAKSLDDRFIIKQVTKTELESFIKFGPAYFKYLTESISTKSPTSLAKIL 1570

Query: 1416 GIYQVTVREPKSGKEVRHELMVMENLSFGRNIIRQYDLKGALHARYNPATNDFGEVLLDQ 1475
            GIYQV+ +  K GKE + +++VMENL F RN  R YDLKG+  ARYNP T+    VLLDQ
Sbjct: 1571 GIYQVSSKHLKGGKEFKMDVLVMENLLFKRNFTRLYDLKGSTRARYNPDTSGSNTVLLDQ 1630

Query: 1476 NFVNDMNSSPLYVNNQAKRRLQRAIWNDTAFLNSMNVMDYSLLVGVDAKKKELVCGIIDY 1490
            N V  M +SP++V ++AKR L+RA+WNDT+FL S++VMDYSLLVGVD ++ ELV GIID+
Sbjct: 1631 NLVEAMPTSPIFVGSKAKRLLERAVWNDTSFLASIHVMDYSLLVGVDEERNELVLGIIDF 1690

BLAST of CmaCh09G011720 vs. ExPASy Swiss-Prot
Match: Q9SSJ8 (Putative 1-phosphatidylinositol-3-phosphate 5-kinase FAB1C OS=Arabidopsis thaliana OX=3702 GN=FAB1C PE=2 SV=1)

HSP 1 Score: 734.6 bits (1895), Expect = 2.3e-210
Identity = 506/1437 (35.21%), Postives = 780/1437 (54.28%), Query Frame = 0

Query: 156  LWDPPEPEDPQDHVEGG-MGYDDDDDDEFDDSTEWNTSSSFSRSVDEASISYRIKEQKQR 215
            +W PP PED  D  E     YDD+DDD  D +TE++ SSSFS  +       ++ E    
Sbjct: 263  IWYPPPPEDENDDAESNYFHYDDEDDDIGDSATEFSLSSSFSSHIPTKE---KLGENSNE 322

Query: 216  AMQQIMNGKFKVFIGHLLKFVGVASSGEGSE-NWVDIVSSLSWEAATFLKPVVN-GKAVD 275
             ++ +++  F+  +  LL+   ++ S +GS   W+DIV++L+W+AA F+KP    G ++D
Sbjct: 323  PLRTVVHDHFRALVAELLRGEELSPSDDGSAGEWLDIVTALAWQAANFVKPDTRAGGSMD 382

Query: 276  PEAHVKVKCIATGTRSQSQFVKGMVFKKHAAHKHMPTHCKNPKLILIEGMLGEAPIS-RL 335
            P  +VK+KC+A+G +++S  ++G+V  K+  HK M +  KNP+++L+ G L    ++ +L
Sbjct: 383  PGNYVKIKCVASGNQNESILIRGIVCSKNITHKRMISQYKNPRVMLLAGSLEYQRVAGQL 442

Query: 336  SSFNS-MDQENDFTKHVIEMIEVCTANVILVEKTTSRVIQEAMLKKGMTLVLDMKLHRLE 395
            +SFN+ + QEN+  K +I  IE    NV+LVEK+ S   Q+ +L+K ++LVL++K   L+
Sbjct: 443  ASFNTLLQQENEHMKAIIAKIESLRPNVLLVEKSASSYAQQYLLEKEISLVLNVKRSLLD 502

Query: 396  RIALCTGSPILTS-ETLMSQNTRQCDAVYFEKIVEEHAGLLEGGKRPTKTLMFIEGCPTR 455
            RIA CTG+ +  S +++ +     C+    E+++E+H    +  ++P++TLM+ EGCP R
Sbjct: 503  RIARCTGAVLCPSLDSISTARLGHCELFRTERVLEQHEAGNQSNRKPSRTLMYFEGCPRR 562

Query: 456  LGCTILLKGAHSDELKKIKVVVQIAVVMAFHLMLETSFLVDQRAMFATIPF--GGVPAT- 515
            LGCT++L+G+  +ELKK+K V+Q AV  A+HL LETSFL D+ A    I     G+  T 
Sbjct: 563  LGCTVVLRGSCREELKKVKHVIQYAVFAAYHLSLETSFLADEGASLPKIRLKQPGMVRTA 622

Query: 516  ---------VSSDPQSPLEPCSPNAQGASNGSNLEGEPY--------------NAAIFSG 575
                     +S   QSP E  S      +   +    P                  IF  
Sbjct: 623  SQRRIIDEGISLITQSPTETDSQALLETAAHEDEHTAPMPEHEVCESLCEDFDPTQIFPP 682

Query: 576  LSSISDSLKRVMGESFFLASPCQSFSSYFGHGKDL-SGLVAKSDSIPSTPPEADQFDVEV 635
             S +       +   F      +S+SS      DL    +  S  IP TP +    + E 
Sbjct: 683  SSEVETEQSDTLNGDFANNLVTRSYSS--NQLNDLHEPTLCLSSEIPETPTQQPSGE-ED 742

Query: 636  RGSSDEENS-IHEQFVPHQSTFDEGFGFHETAPNYSEDKLPKKTPFNSQSILVLMSSRNA 695
             G  +EEN  ++ Q +P   +F E     + +  Y           + QSILV  SSR  
Sbjct: 743  NGRGEEENQLVNPQDLPQHESFYE----DDVSSEYF------SAADSHQSILVSFSSRCV 802

Query: 696  LKGTMCEQSHFSHIVFYKNFDAPLGKFLQENLLNQKNVCTVCGELPEAHYYYYAHSRKQL 755
            LK ++CE+S    I FY +FD PLG++L+++L ++ + C  C EL +AH   Y+H    L
Sbjct: 803  LKESVCERSRLLRIKFYGSFDKPLGRYLKDDLFDKTSSCRSCKELVDAHVLCYSHQNGNL 862

Query: 756  CIQVKQLPVDKVLPGETEGKLWMWSRCSKCKSKGGPSKSTKRVLISTAARGLSFGKFLEL 815
             I V++LP  K LPGE +GK+WMW RC +C    G   +T+RV++S AA GLSFGKFLEL
Sbjct: 863  TINVRRLPSMK-LPGEQDGKIWMWHRCLRCAHVDGVPPATRRVVMSDAAWGLSFGKFLEL 922

Query: 816  CFSDCTLPSKSSVCGHSLFGDFLYFFGLGNMVAMFRYTEVAIYTVSMPPQKLEFNNSMRQ 875
             FS+    ++ + CGHSL  D L F+G GNMVA FRY+ + I TV +PP  LEFN+  +Q
Sbjct: 923  SFSNHATANRVASCGHSLQRDCLRFYGFGNMVAFFRYSPINILTVLLPPSMLEFNSHPQQ 982

Query: 876  GHLITETENVYEKGMILFTEIAQFLKKIISERKSSTVND------------FSLLEEMLN 935
              + TE   +  K   ++TEI+  L ++  E KSS +                L+++++ 
Sbjct: 983  EWIRTEAAELVGKMRTMYTEISDMLNRM--EEKSSLLEPEQSEACDLHSRIIGLIDQLV- 1042

Query: 936  EERSEFELNIQ----SSLTKKGNPDFPFHKYLSLNRLLWELLLESCIWDKRLQLLTS--L 995
            +E+ E++  +Q     +L  +G+ D      L LNRL   L++ +  WD +L LL S   
Sbjct: 1043 KEKDEYDDALQPIFEENLQIQGSLDI-----LELNRLRRALMIGAHAWDHQLYLLNSQLK 1102

Query: 996  GVTINSGTSENVEPEPVRHEGLESIAQTDDRNVQQDVSVDENALHSKEIAVEHLDGESDG 1055
              ++     +N    P  H+         DR +Q+    DE    S      H D E++G
Sbjct: 1103 KASVFKTGDDNAPRNPEMHD-----PPKIDRRMQE--GSDERDEQS------HTDSEANG 1162

Query: 1056 DEL---GLPS-ATEVTE------IPIMDDLSPKQLSRQGSLLNGFN----------RRHS 1115
            D      +PS  T ++E      +    +L   +   +    +  N          R  S
Sbjct: 1163 DNKDPENIPSPGTSLSERIDSAWLGSFQNLEKAETIAETEGFSAVNSSLRRLARPIRVQS 1222

Query: 1116 DDEDPQAGRVLSSGDIPSSTGNLTSDKLFWTPFSEIQQMR--MRDIQRSY-------FPE 1175
             D   +    +  G +P S+  L++ + F         +R  + ++ R+Y         +
Sbjct: 1223 FDSAIRFQERIQKG-LPPSSLYLSTLRSFHASGEYRNMVRDPVSNVMRTYSQMLPLEVQK 1282

Query: 1176 LKSISNYTPKLLPTAHEFINKEGQKLHIFLSN-EKFIVSDYEGELSSIVACVLALLKDLS 1235
            L  I    P  + +A +    +G ++ I        +V  Y+ + +S+V+          
Sbjct: 1283 LDLIVGSAPTYISSASQM--ADGARMLIPQRGLNDIVVPVYDDDPASVVSYA-------- 1342

Query: 1236 LETDLHNEDSKGEGLNK-VPSSGSSDSDSSVTSEEHQFSSFDRLNL-LDSL--------- 1295
                +++++ K   +NK + SS SS + ++  SE   FS++  L++ +D +         
Sbjct: 1343 ----INSKEYKEWIVNKGLASSSSSSNLNNRESEPSAFSTWRSLSMDVDYIQHAVYGSSQ 1402

Query: 1296 -------LPKTFKRAEHEGVIKSLAKGKYLVNCPYFNQFRDLRRRCCPSELHYIASLSRC 1355
                   L  +F          +  K K+ V C +  QF  LR+ CCPSE+ ++ SLSRC
Sbjct: 1403 DDRKSPHLTISFSDRASSSSTATEGKVKFSVTCYFATQFDTLRKTCCPSEVDFVRSLSRC 1462

Query: 1356 INWNAKGGKSKSFFAKTLDDRFIIKEIKRTEYESFMKFAPDYFKYINESFDMGNQTCLAK 1415
              W+A+GGKS  +FAK+LD+RFIIK++ +TE +SF  FAP+YFKY+ ES   G+ TCLAK
Sbjct: 1463 QRWSAQGGKSNVYFAKSLDERFIIKQVVKTELDSFEDFAPEYFKYLKESLSSGSPTCLAK 1522

Query: 1416 VLGIYQVTVREPKSGKEVRHELMVMENLSFGRNIIRQYDLKGALHARYNPATNDFGEVLL 1475
            +LGIYQV+++ PK GKE + +LMVMENL + R I R YDLKG+  +RYNP T+   +VLL
Sbjct: 1523 ILGIYQVSIKHPKGGKETKMDLMVMENLFYNRRISRIYDLKGSARSRYNPNTSGADKVLL 1582

Query: 1476 DQNFVNDMNSSPLYVNNQAKRRLQRAIWNDTAFLNSMNVMDYSLLVGVDAKKKELVCGII 1490
            D N +  + + P+++ ++AKR L+RAIWNDT FL S++VMDYSLLVG D ++KELV GII
Sbjct: 1583 DMNLLETLRTEPIFLGSKAKRSLERAIWNDTNFLASVDVMDYSLLVGFDEERKELVLGII 1642

BLAST of CmaCh09G011720 vs. ExPASy Swiss-Prot
Match: O59722 (1-phosphatidylinositol 3-phosphate 5-kinase fab1 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=fab1 PE=1 SV=2)

HSP 1 Score: 325.9 bits (834), Expect = 2.5e-87
Identity = 373/1485 (25.12%), Postives = 620/1485 (41.75%), Query Frame = 0

Query: 227  FIGHLLKFVGVASSGEGSENWVDIVSSLSWEAATFLKP-VVNGKAVDPEAHVKVKCIATG 286
            F+G LL  +      +    WV  +  +  + A  L P + +G  +D  ++VK+K I  G
Sbjct: 474  FLGKLLFQMLNDEGVDSPMQWVLCLPKILLKMALDLGPDIRSGDDIDVRSYVKIKKIPGG 533

Query: 287  TRSQSQFVKGMVFKKHAAHKHMPTHCKNPKLILIEGMLGEAPISR--LSSFNSMDQENDF 346
            +      V G++F K A+ K M    + P++ L+   L  A   +  LS    + Q+ ++
Sbjct: 534  SIQDCFLVNGVLFSKKASSKSMDRSLRRPRIALLTFSLDYACDEQRILSLDLIISQQEEY 593

Query: 347  TKHVIEMIEVCTANVILVEKTTSRVIQEAMLKKGMTLVLDMKLHRLERIALCTGSPILTS 406
              +++  I +   N++  +     +  +   + G+     +K   L  IA C  + I++S
Sbjct: 594  IINLVNRICMLKPNLVFAQGQIPSIALKYFEEHGVIAFHGLKESVLYDIARCCRADIISS 653

Query: 407  ETLMSQNTRQCDAVYFE---KIVEEHAGLLEGGKRPTKTLMFIEGCPTRLGCTILLKGAH 466
               +S   R      F+    +V+E+ GL        KT   ++ C  RLGCTI+L+GA 
Sbjct: 654  IDKLSLCPRLGTCGRFQLRTYVVDENKGL-------RKTFAILDRCSERLGCTIVLRGAD 713

Query: 467  SDELKKIKVVVQIAVVMAFHLMLETSFLVDQ--------RAMFATIPFGGVPA------- 526
             ++L K+K +V++ V++A+H+ LE + L D+           + ++    +P+       
Sbjct: 714  YNQLSKVKKIVELVVLIAYHIKLECALLRDKFVNMPELFETTYQSLSRKSLPSFASTAAD 773

Query: 527  ----------TVSSDPQSPLEPCSPNAQGASN--GSNLEGEPYNAAIFS-GLSSISDSLK 586
                      +++SD QS L P     Q  S+  GSN   E  N    S   SSI+ +LK
Sbjct: 774  KEKSQNHEKKSLNSDNQS-LRPLENENQSVSSTQGSNSPLELINNLPASDDYSSITKALK 833

Query: 587  -RVMGESFFLASPCQSFSSYFGHGKDLSGLVAKSDSIPSTPPEADQFDVEVRGSSDEENS 646
             R +  S FL+ P     +   + + +   + K   +    P    F ++   + + E S
Sbjct: 834  TRFLTFSPFLSKPLPRLLNQVNYYQFIRNKLLKDVKLHPYSP-TGSFVMKQSENDNVEES 893

Query: 647  IHEQFVPHQSTFDEGFGFHET--APNYSEDKLPKKTPFNSQSILVLMSSRNALKGTMCEQ 706
              E +       DE + F E      YS  KL   +PF+SQ I++L S  N      C  
Sbjct: 894  YEESY--KFFCIDERYHFLEKQWTLYYSHSKL-MFSPFSSQRIILLYSIINKETSVPCIG 953

Query: 707  SHFSHIVFYKNFDAPLGKFLQENLLNQKNVCTVCGELPEA-----HYYYYAHSRKQLCIQ 766
                 + FY+  D  LG++++++ LN    C   GE  +      HY  Y H   ++ + 
Sbjct: 954  PERCLLEFYRETDCTLGQYIEDSCLNTNVSCG--GEYCKTNDMLWHYRSYVHGNSRISVF 1013

Query: 767  VKQLPVDKVLPGETEGKLWMWSRCSKCKSKGGPSKSTKRVLISTAARGLSFGKFLELCFS 826
            ++       +PG  E K+ MWS C  CK      K+T   ++S      SFGK+LE  F 
Sbjct: 1014 LESFSCP--VPG-LEEKIIMWSYCKFCK------KNTHITVMSEETWKYSFGKYLEFMFY 1073

Query: 827  DCTLPSKSSVCGHSLFGDFLYFFGLGNMVAMFRYTEVAIYTVSMPPQKLEFNNSMRQGHL 886
            +  +  +   C HS+    +++FG  NM   F+   + I+ + +P   L  N S  +   
Sbjct: 1074 NSQIRDRFEFCDHSVMAQHVHYFGYCNMALRFQRDLIEIFELFVPSVTLRNNPSYIKE-- 1133

Query: 887  ITETENVYEKGMI--LFTEIAQFLKKI------ISERKSSTVNDFSLLEEMLNEERSEFE 946
            + E E    KG+I    + +A  + +I        E+  S  ++ S    +L+ + +E  
Sbjct: 1134 LKEKEYKRLKGVIEKCLSSVASRINQIKCDWVTDPEKFESCTSEISKFRTLLSSDYTELY 1193

Query: 947  LNIQSSLTKKGNPDFPFHKYLSLNRLLWELLLESCIWDKRL----QLLTSLGVTINSGTS 1006
                S        D     YLSLN +L  L  +   W++R     +L       ++   +
Sbjct: 1194 SEFDSIYLNSSTSD-----YLSLNSILRVLQGKMVKWEQRFLDYQRLYLPSYKELSKIAA 1253

Query: 1007 ENVE---------------PEPVRHEGLESIA--QTDDRNVQQDVSVDENALHSKEIAVE 1066
              ++               PE + +  ++     +T+  + Q +     N   +K +A  
Sbjct: 1254 AQIKKVFLERPLSQTPLDLPETLENTQIDIYPSFKTESTDDQLEKVTQTNVASNKRVA-P 1313

Query: 1067 HLDG-------ESDGDELGLPSATEVTEIPIMDDLSPKQLSRQGSLLN-GFNRRHSDDED 1126
            + D        ESD   +   S      I   +D+S +       + N GF+R+   +  
Sbjct: 1314 YADSMANVGSPESDCFSVATSSDIPKANIDFTNDISTQNTFPASPVSNSGFSRQTYPNIS 1373

Query: 1127 PQAGRVLSSGDIPSSTGNLTSDKLFWTPFSEIQQMRMRDIQRSYFPELKSISN---YTP- 1186
             + G  + S    S++   TSD+ F    S    M M  I  S   ++ SI N   Y+P 
Sbjct: 1374 QRQGVNMLSHKRKSAS---TSDRRF-VNASSTSGMNM-PISSSISAKISSIQNSTKYSPR 1433

Query: 1187 KLLPTAHEFIN-------------KEGQK----------------------LHIF----- 1246
            K +P     ++             +E QK                      + +F     
Sbjct: 1434 KPIPAKDVRVSSLVRRFEELSLQLQEKQKRDEELIKARRKRALPVVPSKPVVEVFNDLNE 1493

Query: 1247 -------------------------------LSNEKFIVSDYEGELSSIVACVLALL--- 1306
                                           +S E+  VS  E         +  +L   
Sbjct: 1494 AFDDENSEDENGINDTKENRATESNFSGVDSMSKERENVSSNEDNSPEAFEDIFGILFKN 1553

Query: 1307 -KDLSLETDLHNEDSKGEGLNKVPSSG--------------------------------- 1366
               L  + +L       +  +K+P+SG                                 
Sbjct: 1554 ESGLEEQQNLEPSSQMDKEGSKLPTSGPLADKTSVYRILSAFWNEWNSLNPPPFEFPLQP 1613

Query: 1367 --SSDSDSSVTSEEHQFSSFDRLNL---------------LDSLLPKTFKRAEHEGVIKS 1426
                 SDS+V   E + SS     L               +D  L     +   +  I++
Sbjct: 1614 TEHMFSDSNVIIREDEPSSLISFTLSSPDYLSKMVEIEDSMDEALTNQGLQGSTQFKIEN 1673

Query: 1427 L-------------AKGKYLVNCPYF--NQFRDLRRRCCPSELHYIASLSRCINWNAKGG 1486
            L              +G   ++C  F   QF  L RR C  E  ++ SL+RC  W + GG
Sbjct: 1674 LMLKPTGTHLKYQFEEGSARLSCKVFFAEQFSAL-RRACGCEETFVTSLARCSLWESSGG 1733

Query: 1487 KSKSFFAKTLDDRFIIKEIKRTEYESFMKFAPDYFKYINESFDMGNQTCLAKVLGIYQVT 1488
            KS S F KT D ++I+K + R E +S + FAP YF YI++ F     T L K+ G Y+V 
Sbjct: 1734 KSGSAFLKTFDKKYILKVLSRLESDSLLNFAPAYFDYISKVFFHELPTALTKIFGFYRVD 1793

BLAST of CmaCh09G011720 vs. ExPASy TrEMBL
Match: A0A6J1IGZ0 (1-phosphatidylinositol-3-phosphate 5-kinase OS=Cucurbita maxima OX=3661 GN=LOC111477135 PE=4 SV=1)

HSP 1 Score: 3038.8 bits (7877), Expect = 0.0e+00
Identity = 1522/1522 (100.00%), Postives = 1522/1522 (100.00%), Query Frame = 0

Query: 1    MCHYCGAVITDSEGKRLGNGNSFDLDDSGTVCICRVCKENQELEAMNLDNKSSSEALMLS 60
            MCHYCGAVITDSEGKRLGNGNSFDLDDSGTVCICRVCKENQELEAMNLDNKSSSEALMLS
Sbjct: 1    MCHYCGAVITDSEGKRLGNGNSFDLDDSGTVCICRVCKENQELEAMNLDNKSSSEALMLS 60

Query: 61   PMPSLSSCDSDCSTVANLDIKDGQEGTTRSSQDSIDCRQERMSQNWSEVVHSNGQLSGRD 120
            PMPSLSSCDSDCSTVANLDIKDGQEGTTRSSQDSIDCRQERMSQNWSEVVHSNGQLSGRD
Sbjct: 61   PMPSLSSCDSDCSTVANLDIKDGQEGTTRSSQDSIDCRQERMSQNWSEVVHSNGQLSGRD 120

Query: 121  DESVITISQEATHNGSGVRVNAEIEQSHSNWLDTDLWDPPEPEDPQDHVEGGMGYDDDDD 180
            DESVITISQEATHNGSGVRVNAEIEQSHSNWLDTDLWDPPEPEDPQDHVEGGMGYDDDDD
Sbjct: 121  DESVITISQEATHNGSGVRVNAEIEQSHSNWLDTDLWDPPEPEDPQDHVEGGMGYDDDDD 180

Query: 181  DEFDDSTEWNTSSSFSRSVDEASISYRIKEQKQRAMQQIMNGKFKVFIGHLLKFVGVASS 240
            DEFDDSTEWNTSSSFSRSVDEASISYRIKEQKQRAMQQIMNGKFKVFIGHLLKFVGVASS
Sbjct: 181  DEFDDSTEWNTSSSFSRSVDEASISYRIKEQKQRAMQQIMNGKFKVFIGHLLKFVGVASS 240

Query: 241  GEGSENWVDIVSSLSWEAATFLKPVVNGKAVDPEAHVKVKCIATGTRSQSQFVKGMVFKK 300
            GEGSENWVDIVSSLSWEAATFLKPVVNGKAVDPEAHVKVKCIATGTRSQSQFVKGMVFKK
Sbjct: 241  GEGSENWVDIVSSLSWEAATFLKPVVNGKAVDPEAHVKVKCIATGTRSQSQFVKGMVFKK 300

Query: 301  HAAHKHMPTHCKNPKLILIEGMLGEAPISRLSSFNSMDQENDFTKHVIEMIEVCTANVIL 360
            HAAHKHMPTHCKNPKLILIEGMLGEAPISRLSSFNSMDQENDFTKHVIEMIEVCTANVIL
Sbjct: 301  HAAHKHMPTHCKNPKLILIEGMLGEAPISRLSSFNSMDQENDFTKHVIEMIEVCTANVIL 360

Query: 361  VEKTTSRVIQEAMLKKGMTLVLDMKLHRLERIALCTGSPILTSETLMSQNTRQCDAVYFE 420
            VEKTTSRVIQEAMLKKGMTLVLDMKLHRLERIALCTGSPILTSETLMSQNTRQCDAVYFE
Sbjct: 361  VEKTTSRVIQEAMLKKGMTLVLDMKLHRLERIALCTGSPILTSETLMSQNTRQCDAVYFE 420

Query: 421  KIVEEHAGLLEGGKRPTKTLMFIEGCPTRLGCTILLKGAHSDELKKIKVVVQIAVVMAFH 480
            KIVEEHAGLLEGGKRPTKTLMFIEGCPTRLGCTILLKGAHSDELKKIKVVVQIAVVMAFH
Sbjct: 421  KIVEEHAGLLEGGKRPTKTLMFIEGCPTRLGCTILLKGAHSDELKKIKVVVQIAVVMAFH 480

Query: 481  LMLETSFLVDQRAMFATIPFGGVPATVSSDPQSPLEPCSPNAQGASNGSNLEGEPYNAAI 540
            LMLETSFLVDQRAMFATIPFGGVPATVSSDPQSPLEPCSPNAQGASNGSNLEGEPYNAAI
Sbjct: 481  LMLETSFLVDQRAMFATIPFGGVPATVSSDPQSPLEPCSPNAQGASNGSNLEGEPYNAAI 540

Query: 541  FSGLSSISDSLKRVMGESFFLASPCQSFSSYFGHGKDLSGLVAKSDSIPSTPPEADQFDV 600
            FSGLSSISDSLKRVMGESFFLASPCQSFSSYFGHGKDLSGLVAKSDSIPSTPPEADQFDV
Sbjct: 541  FSGLSSISDSLKRVMGESFFLASPCQSFSSYFGHGKDLSGLVAKSDSIPSTPPEADQFDV 600

Query: 601  EVRGSSDEENSIHEQFVPHQSTFDEGFGFHETAPNYSEDKLPKKTPFNSQSILVLMSSRN 660
            EVRGSSDEENSIHEQFVPHQSTFDEGFGFHETAPNYSEDKLPKKTPFNSQSILVLMSSRN
Sbjct: 601  EVRGSSDEENSIHEQFVPHQSTFDEGFGFHETAPNYSEDKLPKKTPFNSQSILVLMSSRN 660

Query: 661  ALKGTMCEQSHFSHIVFYKNFDAPLGKFLQENLLNQKNVCTVCGELPEAHYYYYAHSRKQ 720
            ALKGTMCEQSHFSHIVFYKNFDAPLGKFLQENLLNQKNVCTVCGELPEAHYYYYAHSRKQ
Sbjct: 661  ALKGTMCEQSHFSHIVFYKNFDAPLGKFLQENLLNQKNVCTVCGELPEAHYYYYAHSRKQ 720

Query: 721  LCIQVKQLPVDKVLPGETEGKLWMWSRCSKCKSKGGPSKSTKRVLISTAARGLSFGKFLE 780
            LCIQVKQLPVDKVLPGETEGKLWMWSRCSKCKSKGGPSKSTKRVLISTAARGLSFGKFLE
Sbjct: 721  LCIQVKQLPVDKVLPGETEGKLWMWSRCSKCKSKGGPSKSTKRVLISTAARGLSFGKFLE 780

Query: 781  LCFSDCTLPSKSSVCGHSLFGDFLYFFGLGNMVAMFRYTEVAIYTVSMPPQKLEFNNSMR 840
            LCFSDCTLPSKSSVCGHSLFGDFLYFFGLGNMVAMFRYTEVAIYTVSMPPQKLEFNNSMR
Sbjct: 781  LCFSDCTLPSKSSVCGHSLFGDFLYFFGLGNMVAMFRYTEVAIYTVSMPPQKLEFNNSMR 840

Query: 841  QGHLITETENVYEKGMILFTEIAQFLKKIISERKSSTVNDFSLLEEMLNEERSEFELNIQ 900
            QGHLITETENVYEKGMILFTEIAQFLKKIISERKSSTVNDFSLLEEMLNEERSEFELNIQ
Sbjct: 841  QGHLITETENVYEKGMILFTEIAQFLKKIISERKSSTVNDFSLLEEMLNEERSEFELNIQ 900

Query: 901  SSLTKKGNPDFPFHKYLSLNRLLWELLLESCIWDKRLQLLTSLGVTINSGTSENVEPEPV 960
            SSLTKKGNPDFPFHKYLSLNRLLWELLLESCIWDKRLQLLTSLGVTINSGTSENVEPEPV
Sbjct: 901  SSLTKKGNPDFPFHKYLSLNRLLWELLLESCIWDKRLQLLTSLGVTINSGTSENVEPEPV 960

Query: 961  RHEGLESIAQTDDRNVQQDVSVDENALHSKEIAVEHLDGESDGDELGLPSATEVTEIPIM 1020
            RHEGLESIAQTDDRNVQQDVSVDENALHSKEIAVEHLDGESDGDELGLPSATEVTEIPIM
Sbjct: 961  RHEGLESIAQTDDRNVQQDVSVDENALHSKEIAVEHLDGESDGDELGLPSATEVTEIPIM 1020

Query: 1021 DDLSPKQLSRQGSLLNGFNRRHSDDEDPQAGRVLSSGDIPSSTGNLTSDKLFWTPFSEIQ 1080
            DDLSPKQLSRQGSLLNGFNRRHSDDEDPQAGRVLSSGDIPSSTGNLTSDKLFWTPFSEIQ
Sbjct: 1021 DDLSPKQLSRQGSLLNGFNRRHSDDEDPQAGRVLSSGDIPSSTGNLTSDKLFWTPFSEIQ 1080

Query: 1081 QMRMRDIQRSYFPELKSISNYTPKLLPTAHEFINKEGQKLHIFLSNEKFIVSDYEGELSS 1140
            QMRMRDIQRSYFPELKSISNYTPKLLPTAHEFINKEGQKLHIFLSNEKFIVSDYEGELSS
Sbjct: 1081 QMRMRDIQRSYFPELKSISNYTPKLLPTAHEFINKEGQKLHIFLSNEKFIVSDYEGELSS 1140

Query: 1141 IVACVLALLKDLSLETDLHNEDSKGEGLNKVPSSGSSDSDSSVTSEEHQFSSFDRLNLLD 1200
            IVACVLALLKDLSLETDLHNEDSKGEGLNKVPSSGSSDSDSSVTSEEHQFSSFDRLNLLD
Sbjct: 1141 IVACVLALLKDLSLETDLHNEDSKGEGLNKVPSSGSSDSDSSVTSEEHQFSSFDRLNLLD 1200

Query: 1201 SLLPKTFKRAEHEGVIKSLAKGKYLVNCPYFNQFRDLRRRCCPSELHYIASLSRCINWNA 1260
            SLLPKTFKRAEHEGVIKSLAKGKYLVNCPYFNQFRDLRRRCCPSELHYIASLSRCINWNA
Sbjct: 1201 SLLPKTFKRAEHEGVIKSLAKGKYLVNCPYFNQFRDLRRRCCPSELHYIASLSRCINWNA 1260

Query: 1261 KGGKSKSFFAKTLDDRFIIKEIKRTEYESFMKFAPDYFKYINESFDMGNQTCLAKVLGIY 1320
            KGGKSKSFFAKTLDDRFIIKEIKRTEYESFMKFAPDYFKYINESFDMGNQTCLAKVLGIY
Sbjct: 1261 KGGKSKSFFAKTLDDRFIIKEIKRTEYESFMKFAPDYFKYINESFDMGNQTCLAKVLGIY 1320

Query: 1321 QVTVREPKSGKEVRHELMVMENLSFGRNIIRQYDLKGALHARYNPATNDFGEVLLDQNFV 1380
            QVTVREPKSGKEVRHELMVMENLSFGRNIIRQYDLKGALHARYNPATNDFGEVLLDQNFV
Sbjct: 1321 QVTVREPKSGKEVRHELMVMENLSFGRNIIRQYDLKGALHARYNPATNDFGEVLLDQNFV 1380

Query: 1381 NDMNSSPLYVNNQAKRRLQRAIWNDTAFLNSMNVMDYSLLVGVDAKKKELVCGIIDYLRQ 1440
            NDMNSSPLYVNNQAKRRLQRAIWNDTAFLNSMNVMDYSLLVGVDAKKKELVCGIIDYLRQ
Sbjct: 1381 NDMNSSPLYVNNQAKRRLQRAIWNDTAFLNSMNVMDYSLLVGVDAKKKELVCGIIDYLRQ 1440

Query: 1441 YTWDKQLETWAKSSLRPKNVLPTVVSPKEYKRRFRKFMSAHFLSVPDNWCQQQNLHGPWG 1500
            YTWDKQLETWAKSSLRPKNVLPTVVSPKEYKRRFRKFMSAHFLSVPDNWCQQQNLHGPWG
Sbjct: 1441 YTWDKQLETWAKSSLRPKNVLPTVVSPKEYKRRFRKFMSAHFLSVPDNWCQQQNLHGPWG 1500

Query: 1501 ACGGTIDHASSQTNAEENDLSS 1523
            ACGGTIDHASSQTNAEENDLSS
Sbjct: 1501 ACGGTIDHASSQTNAEENDLSS 1522

BLAST of CmaCh09G011720 vs. ExPASy TrEMBL
Match: A0A6J1EWK7 (1-phosphatidylinositol-3-phosphate 5-kinase OS=Cucurbita moschata OX=3662 GN=LOC111439025 PE=4 SV=1)

HSP 1 Score: 2989.5 bits (7749), Expect = 0.0e+00
Identity = 1494/1522 (98.16%), Postives = 1510/1522 (99.21%), Query Frame = 0

Query: 1    MCHYCGAVITDSEGKRLGNGNSFDLDDSGTVCICRVCKENQELEAMNLDNKSSSEALMLS 60
            MCHYCGAVITDSEGKRLGNGNSFDLDDSGTVCICRVCKENQEL+ MNLDNKSSSEALMLS
Sbjct: 1    MCHYCGAVITDSEGKRLGNGNSFDLDDSGTVCICRVCKENQELKTMNLDNKSSSEALMLS 60

Query: 61   PMPSLSSCDSDCSTVANLDIKDGQEGTTRSSQDSIDCRQERMSQNWSEVVHSNGQLSGRD 120
            PMPSLSSCDSDCSTVANLD+KDGQ+GTTRSSQDSIDCRQERMSQNWSEVVHSNGQLSGRD
Sbjct: 61   PMPSLSSCDSDCSTVANLDVKDGQDGTTRSSQDSIDCRQERMSQNWSEVVHSNGQLSGRD 120

Query: 121  DESVITISQEATHNGSGVRVNAEIEQSHSNWLDTDLWDPPEPEDPQDHVEGGMGYDDDDD 180
            DESVITISQEATHNGSGVRVNAEIEQSHSNWLDTDLWDPPEPEDPQDH+EGGMGYDDDDD
Sbjct: 121  DESVITISQEATHNGSGVRVNAEIEQSHSNWLDTDLWDPPEPEDPQDHMEGGMGYDDDDD 180

Query: 181  DEFDDSTEWNTSSSFSRSVDEASISYRIKEQKQRAMQQIMNGKFKVFIGHLLKFVGVASS 240
            DEFDDSTEWNTSSSFSRSVDEAS+SYRIKEQKQRAMQQIMNGKFKVFIGHLLKFVGVASS
Sbjct: 181  DEFDDSTEWNTSSSFSRSVDEASVSYRIKEQKQRAMQQIMNGKFKVFIGHLLKFVGVASS 240

Query: 241  GEGSENWVDIVSSLSWEAATFLKPVVNGKAVDPEAHVKVKCIATGTRSQSQFVKGMVFKK 300
            GE SENWVDIVSSLSWEAATFLKPVVNGKAVDPEAHVKVKCIATGTRSQSQFVKGMVFKK
Sbjct: 241  GEDSENWVDIVSSLSWEAATFLKPVVNGKAVDPEAHVKVKCIATGTRSQSQFVKGMVFKK 300

Query: 301  HAAHKHMPTHCKNPKLILIEGMLGEAPISRLSSFNSMDQENDFTKHVIEMIEVCTANVIL 360
            HAAHKHMPTHCKNPKLILIEGMLGEAPISRLSSFNSMDQENDFTKHVIEMIEVCTANVIL
Sbjct: 301  HAAHKHMPTHCKNPKLILIEGMLGEAPISRLSSFNSMDQENDFTKHVIEMIEVCTANVIL 360

Query: 361  VEKTTSRVIQEAMLKKGMTLVLDMKLHRLERIALCTGSPILTSETLMSQNTRQCDAVYFE 420
            VEKTTSRVIQEAMLKKGMTLVLDMKLHRLERIALCTGSPILTSETLMSQNTRQCDAVYFE
Sbjct: 361  VEKTTSRVIQEAMLKKGMTLVLDMKLHRLERIALCTGSPILTSETLMSQNTRQCDAVYFE 420

Query: 421  KIVEEHAGLLEGGKRPTKTLMFIEGCPTRLGCTILLKGAHSDELKKIKVVVQIAVVMAFH 480
            KIVEEHAGLLEGGKRPTKTLMFIEGCPTRLGCTILLKGAHSDELKKIKVVVQIAVVMAFH
Sbjct: 421  KIVEEHAGLLEGGKRPTKTLMFIEGCPTRLGCTILLKGAHSDELKKIKVVVQIAVVMAFH 480

Query: 481  LMLETSFLVDQRAMFATIPFGGVPATVSSDPQSPLEPCSPNAQGASNGSNLEGEPYNAAI 540
            LMLETSFLVDQRAMFATIPFGGVPATVSSDPQSPLEPCSPNAQGASNGSNLEGEPYN AI
Sbjct: 481  LMLETSFLVDQRAMFATIPFGGVPATVSSDPQSPLEPCSPNAQGASNGSNLEGEPYNPAI 540

Query: 541  FSGLSSISDSLKRVMGESFFLASPCQSFSSYFGHGKDLSGLVAKSDSIPSTPPEADQFDV 600
            FSGLSSISDSLKRVMGESFFLASPCQSFSSYFGHGKDLSGLVAKSDSIPSTPPEADQFDV
Sbjct: 541  FSGLSSISDSLKRVMGESFFLASPCQSFSSYFGHGKDLSGLVAKSDSIPSTPPEADQFDV 600

Query: 601  EVRGSSDEENSIHEQFVPHQSTFDEGFGFHETAPNYSEDKLPKKTPFNSQSILVLMSSRN 660
            EVRGSSDEENSIHEQFVPHQSTFDEGFGFHETAPNYSEDKLP+KTPFNSQSILVLMSSRN
Sbjct: 601  EVRGSSDEENSIHEQFVPHQSTFDEGFGFHETAPNYSEDKLPEKTPFNSQSILVLMSSRN 660

Query: 661  ALKGTMCEQSHFSHIVFYKNFDAPLGKFLQENLLNQKNVCTVCGELPEAHYYYYAHSRKQ 720
            A KGTMCEQSHFSHIVFYKNFDAPLGKFLQENLLNQKNVCTVCGELPEAHYYYYAHSRKQ
Sbjct: 661  ASKGTMCEQSHFSHIVFYKNFDAPLGKFLQENLLNQKNVCTVCGELPEAHYYYYAHSRKQ 720

Query: 721  LCIQVKQLPVDKVLPGETEGKLWMWSRCSKCKSKGGPSKSTKRVLISTAARGLSFGKFLE 780
            LCIQVKQLPV+KVLPGETEGKLWMWSRCSKCKSKGGPSKSTKRVLISTAARGLSFGKFLE
Sbjct: 721  LCIQVKQLPVNKVLPGETEGKLWMWSRCSKCKSKGGPSKSTKRVLISTAARGLSFGKFLE 780

Query: 781  LCFSDCTLPSKSSVCGHSLFGDFLYFFGLGNMVAMFRYTEVAIYTVSMPPQKLEFNNSMR 840
            LCFSDCTLPSKSSVCGHSLFGDFLYFFGLGNMVAMFRYTEVAIYTVSMPPQKLEFN+SMR
Sbjct: 781  LCFSDCTLPSKSSVCGHSLFGDFLYFFGLGNMVAMFRYTEVAIYTVSMPPQKLEFNSSMR 840

Query: 841  QGHLITETENVYEKGMILFTEIAQFLKKIISERKSSTVNDFSLLEEMLNEERSEFELNIQ 900
            QGHLITETENVY KGMILFTEIAQFLKKIISERKSSTVNDFSLLEEMLNEERSEFELNIQ
Sbjct: 841  QGHLITETENVYAKGMILFTEIAQFLKKIISERKSSTVNDFSLLEEMLNEERSEFELNIQ 900

Query: 901  SSLTKKGNPDFPFHKYLSLNRLLWELLLESCIWDKRLQLLTSLGVTINSGTSENVEPEPV 960
            SSLTKKGNPDFPFHKYLSLNRLLWELLLESCIWDKRLQLLTS+GVT+NSGTSENVEPEPV
Sbjct: 901  SSLTKKGNPDFPFHKYLSLNRLLWELLLESCIWDKRLQLLTSVGVTLNSGTSENVEPEPV 960

Query: 961  RHEGLESIAQTDDRNVQQDVSVDENALHSKEIAVEHLDGESDGDELGLPSATEVTEIPIM 1020
              EGLESIAQTDDRNVQQDVSVDENALH KEIAVEHLDGESDGDELGLPSATEVTEIPIM
Sbjct: 961  GQEGLESIAQTDDRNVQQDVSVDENALHPKEIAVEHLDGESDGDELGLPSATEVTEIPIM 1020

Query: 1021 DDLSPKQLSRQGSLLNGFNRRHSDDEDPQAGRVLSSGDIPSSTGNLTSDKLFWTPFSEIQ 1080
            DDLSPKQLSRQGSL NGFNRRHSDDEDPQA RVLSSGDIPSSTGNLT DKLFWTPFSEIQ
Sbjct: 1021 DDLSPKQLSRQGSLSNGFNRRHSDDEDPQAVRVLSSGDIPSSTGNLTLDKLFWTPFSEIQ 1080

Query: 1081 QMRMRDIQRSYFPELKSISNYTPKLLPTAHEFINKEGQKLHIFLSNEKFIVSDYEGELSS 1140
            QMRMRDIQRSYFPELKS+SNYTPKLLPTAH+FINKEGQKLHIFLSNEKFIVSDYEGELSS
Sbjct: 1081 QMRMRDIQRSYFPELKSVSNYTPKLLPTAHDFINKEGQKLHIFLSNEKFIVSDYEGELSS 1140

Query: 1141 IVACVLALLKDLSLETDLHNEDSKGEGLNKVPSSGSSDSDSSVTSEEHQFSSFDRLNLLD 1200
            IVACVLALLKDLSLETDLHNEDSKGEGLNKVPS+GSSDSDSSV+SEEHQFSSFDRLNLLD
Sbjct: 1141 IVACVLALLKDLSLETDLHNEDSKGEGLNKVPSNGSSDSDSSVSSEEHQFSSFDRLNLLD 1200

Query: 1201 SLLPKTFKRAEHEGVIKSLAKGKYLVNCPYFNQFRDLRRRCCPSELHYIASLSRCINWNA 1260
            SLLPKTFKRAEHEGVIKSLAKGKYLVNCPYFNQFRDLRRRCCPSELHYIASLSRCI+WNA
Sbjct: 1201 SLLPKTFKRAEHEGVIKSLAKGKYLVNCPYFNQFRDLRRRCCPSELHYIASLSRCIHWNA 1260

Query: 1261 KGGKSKSFFAKTLDDRFIIKEIKRTEYESFMKFAPDYFKYINESFDMGNQTCLAKVLGIY 1320
            KGGKSKSFFAKTLDDRFIIKEIKRTEYESFMKFAPDYFKYINESFDMGNQTCLAKVLGIY
Sbjct: 1261 KGGKSKSFFAKTLDDRFIIKEIKRTEYESFMKFAPDYFKYINESFDMGNQTCLAKVLGIY 1320

Query: 1321 QVTVREPKSGKEVRHELMVMENLSFGRNIIRQYDLKGALHARYNPATNDFGEVLLDQNFV 1380
            QVTVREPKSGKEVRHELMVMENLSFGRNIIRQYDLKGALHARYNPATNDFGEVLLDQNFV
Sbjct: 1321 QVTVREPKSGKEVRHELMVMENLSFGRNIIRQYDLKGALHARYNPATNDFGEVLLDQNFV 1380

Query: 1381 NDMNSSPLYVNNQAKRRLQRAIWNDTAFLNSMNVMDYSLLVGVDAKKKELVCGIIDYLRQ 1440
            NDMNSSPLYVNNQAKRRLQRAIWNDTAFLNSMNVMDYSLLVGVDA+KKELVCGIIDYLRQ
Sbjct: 1381 NDMNSSPLYVNNQAKRRLQRAIWNDTAFLNSMNVMDYSLLVGVDAEKKELVCGIIDYLRQ 1440

Query: 1441 YTWDKQLETWAKSSLRPKNVLPTVVSPKEYKRRFRKFMSAHFLSVPDNWCQQQNLHGPWG 1500
            YTWDKQLETWAKSSLRPKNVLPTVVSPKEYKRRFRKFMSAHFLSVPDNWCQQQNLHGPWG
Sbjct: 1441 YTWDKQLETWAKSSLRPKNVLPTVVSPKEYKRRFRKFMSAHFLSVPDNWCQQQNLHGPWG 1500

Query: 1501 ACGGTIDHASSQTNAEENDLSS 1523
            ACGGTID ASSQTNAEENDLSS
Sbjct: 1501 ACGGTIDRASSQTNAEENDLSS 1522

BLAST of CmaCh09G011720 vs. ExPASy TrEMBL
Match: A0A5D3DIR8 (1-phosphatidylinositol-3-phosphate 5-kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold142G001430 PE=4 SV=1)

HSP 1 Score: 2426.0 bits (6286), Expect = 0.0e+00
Identity = 1258/1604 (78.43%), Postives = 1362/1604 (84.91%), Query Frame = 0

Query: 1    MCHYCGAVITDSEGKRLGNGNSFDLDDSGTVCICRVCKENQELEAMNLDNKSSSEALMLS 60
            MCHYCGAVITDS GK L NG+S  LDD+  +C+CRVCKENQE E M  DN++SSEA  LS
Sbjct: 1    MCHYCGAVITDSNGKNLENGSSLKLDDADPMCLCRVCKENQEQEMMKSDNRNSSEAPTLS 60

Query: 61   PMPSLSSCDS-----------DCSTVANLDIKDGQEGTTRSSQDSIDCRQERMSQNWSEV 120
            PMPSLSSC+S           DCSTVANLD++DGQE  T SSQ+ IDCRQERMS N SEV
Sbjct: 61   PMPSLSSCNSCVSANYTSFKCDCSTVANLDVRDGQESATGSSQEDIDCRQERMSLNSSEV 120

Query: 121  VHSNGQLSGRDDESVITISQEATHNGSGVRVNAEIEQSHSNWLDTDLWDPPEPEDPQDHV 180
              SN QLSGRDD SV++ +QE T N SGV VNA++EQS+SNW+D DLWDPPEPEDP+DH+
Sbjct: 121  ADSNDQLSGRDDGSVMSNNQETTQNASGVPVNADVEQSNSNWMDPDLWDPPEPEDPEDHM 180

Query: 181  EGGMGYDDDDDDEFDDSTEWNTSSSFSRSVDEASISYRIKEQKQRAMQQIMNGKFKVFIG 240
            EGGMGY+DDDDDEF DS+EW+TSSSFSRSVDEAS+SYR KE+KQRAMQQ+MNGK+K FI 
Sbjct: 181  EGGMGYNDDDDDEFGDSSEWSTSSSFSRSVDEASVSYRFKEEKQRAMQQVMNGKYKAFIR 240

Query: 241  HLLKFVGV-ASSGEGSENWVDIVSSLSWEAATFLKPVVNGKAVDPEAHVKVKCIATGTRS 300
            HLLK V V ASSGE SENWVDIVSSLSWEAATFLKPVVNGKA+DP AHVKVKCIATGTR 
Sbjct: 241  HLLKVVHVPASSGEDSENWVDIVSSLSWEAATFLKPVVNGKAMDPNAHVKVKCIATGTRK 300

Query: 301  QSQFVKGMVFKKHAAHKHMPTHCKNPKLILIEGMLGEAPISRLSSFNSMDQENDFTKHVI 360
            QSQFVKGMVFKKHAAHKHMPTHCKNPKL+LI+GMLGEAPISRLSSFNSMDQENDFT HVI
Sbjct: 301  QSQFVKGMVFKKHAAHKHMPTHCKNPKLVLIQGMLGEAPISRLSSFNSMDQENDFTNHVI 360

Query: 361  EMIEVCTANVILVEKTTSRVIQEAMLKKGMTLVLDMKLHRLERIALCTGSPILTSETLMS 420
            EMIE CTANVILVEKT +RVIQEA+LKKGMTLVLDMKLHRL+RIALCTGSPILTSETLMS
Sbjct: 361  EMIEGCTANVILVEKTAARVIQEAILKKGMTLVLDMKLHRLQRIALCTGSPILTSETLMS 420

Query: 421  QNTRQCDAVYFEKIVEEHAGLLEGGKRPTKTLMFIEGCPTRLGCTILLKGAHSDELKKIK 480
            Q TRQCDAVYF+KIVEEHAG+LEGGK  TKTLMFIEGCPTRLGCTILLKGAHSDELK++K
Sbjct: 421  QKTRQCDAVYFQKIVEEHAGVLEGGKMSTKTLMFIEGCPTRLGCTILLKGAHSDELKRVK 480

Query: 481  VVVQIAVVMAFHLMLETSFLVDQRAMFATIPFGGVPATVSSDPQSPLEPCSPNA------ 540
            +VVQ AVVMAF L+LETSF+VDQ+AMFATIPFGGVP+  SSDPQSPLEP SP        
Sbjct: 481  LVVQCAVVMAFQLILETSFIVDQQAMFATIPFGGVPSIASSDPQSPLEPRSPKVGQAESA 540

Query: 541  -----------------QGASNGSNL----------EGEPYNAAIFSGLSSISDSLKRVM 600
                             + ASN SN+          E EPYN AIFSG SSISDSLKR M
Sbjct: 541  SDQIEQPTNIHISSGSHEEASNESNVETVEKLIVSSEPEPYNPAIFSGFSSISDSLKRAM 600

Query: 601  GESFFLASPCQSFSSYFGHGKDLSGLVAKSDSIPSTPPEADQFDVEVRGSSDEENSIHEQ 660
            GESF L SP  S SSYFGH  DLSGLVAKSDSIPST     QFD EVRGSSD ENS+HEQ
Sbjct: 601  GESFLLTSPYLSLSSYFGHESDLSGLVAKSDSIPSTLQAVYQFDEEVRGSSDGENSVHEQ 660

Query: 661  FVPHQSTFDEGFGFHETAPNYSEDKLPKKTPFNSQSILVLMSSRNALKGTMCEQSHFSHI 720
             V HQST  EG GFHETAPN + D + KK P +SQSILVLMSSRNALKGTMCEQSHFSHI
Sbjct: 661  SVSHQSTL-EGLGFHETAPNDTGDTMQKKPPLDSQSILVLMSSRNALKGTMCEQSHFSHI 720

Query: 721  VFYKNFDAPLGKFLQENLLNQKNVCTVCGELPEAHYYYYAHSRKQLCIQVKQLPVDKVLP 780
            VFYKNFD PLGKFLQENLLNQ N+CTVCGELPEAH+YYYAH RKQL IQVKQLP DKVLP
Sbjct: 721  VFYKNFDVPLGKFLQENLLNQNNLCTVCGELPEAHFYYYAHYRKQLSIQVKQLPGDKVLP 780

Query: 781  GETEGKLWMWSRCSKCKSKGGPSKSTKRVLISTAARGLSFGKFLELCFSDCTLPSKSSVC 840
            GETEGKLWMWSRCSKCKS GGPSKSTKRVLISTAARGLSFGKFLELCFSD TLPSKSSVC
Sbjct: 781  GETEGKLWMWSRCSKCKSLGGPSKSTKRVLISTAARGLSFGKFLELCFSDDTLPSKSSVC 840

Query: 841  GHSLFGDFLYFFG--LGNMVAMFRYTEVAIYTVSMPPQKLEFNNSMRQGHLITETENVYE 900
            GHSLFGDFLYFFG  LGNMVAMFRY+EVAIYTVSMPPQKLEFN+SMRQGHLI E ENVY 
Sbjct: 841  GHSLFGDFLYFFGYVLGNMVAMFRYSEVAIYTVSMPPQKLEFNSSMRQGHLIKEIENVYT 900

Query: 901  KGMILFTEIAQFLKKIISERKSSTV-------NDFSLLEEMLNEERSEFELNIQSSLTKK 960
            KGM+LF+EIA  LKKIISER SS V       NDFS +EEMLNEERSEFE+ IQ+SLTKK
Sbjct: 901  KGMLLFSEIAHSLKKIISERNSSNVNHPQGFLNDFSFVEEMLNEERSEFEVKIQNSLTKK 960

Query: 961  GNPDFPFHKYLSLNRLLWELLLESCIWDKRLQLLTSLGVTINSGTSENVEPEPVR----- 1020
            GN D  FHK+L+LNRLLW+LLLESCIWD+RLQ L SLGVT NSGTSENVEPEPV      
Sbjct: 961  GNLDLAFHKFLNLNRLLWDLLLESCIWDRRLQSLASLGVTTNSGTSENVEPEPVMLKMNS 1020

Query: 1021 -----HEGLESIAQTDDRNVQQDVSVDENALHSKEIAVEHLDGESDGDELGLPSATEVTE 1080
                  E  ESIA+ DD  +QQD+SVDEN L  KEIAVE  DGES GDEL LPSA EVTE
Sbjct: 1021 NINVGPEESESIAENDDSKIQQDISVDENVLPLKEIAVEGSDGESGGDELNLPSAIEVTE 1080

Query: 1081 IPIMDDLSPKQLSRQGSLLNGFNRRHSDDEDPQAGRVLSSGD------IPSSTGNLTSDK 1140
            I I+DDLSPK+LSRQG+L NGF+  HSD ED Q GRVLSSGD      IP S G+ T  K
Sbjct: 1081 IQIIDDLSPKKLSRQGTLSNGFDCHHSDYEDSQVGRVLSSGDLHVDRTIPISIGDSTLSK 1140

Query: 1141 LFWTPFSEIQQMRMRDIQRSYFPELKSISNYTPKLLPTAHEFINKEGQKLHIFLSNEKFI 1200
            LF TPFSEI+QM +RDIQRSYFPELKSIS+YTPKLL  A +FI++EGQKLHI L+++ F+
Sbjct: 1141 LFCTPFSEIRQMHLRDIQRSYFPELKSISSYTPKLLTAASDFIHEEGQKLHIHLADDNFV 1200

Query: 1201 VSDYEGELSSIVACVLALLKDLSLETDLHNEDSKGEG------------LNKVPSSGSSD 1260
            VSDYEGELSSIVACVLALLKD   +TD HNEDSKGEG            LNKVPS+GSSD
Sbjct: 1201 VSDYEGELSSIVACVLALLKDQPFQTDSHNEDSKGEGGGALQPALSSRSLNKVPSNGSSD 1260

Query: 1261 SDSSVTSEEHQFSSFDRLNLLDSLLPKTFKRAEHEGVIKSLAKGKYLVNCPYFNQFRDLR 1320
            S+SS++SEE++FSSFDRLNLLDSL+ +TFKR++HEGVIKSLAKGKYLVNCPY NQFRDLR
Sbjct: 1261 SESSISSEEYRFSSFDRLNLLDSLVSETFKRSDHEGVIKSLAKGKYLVNCPYINQFRDLR 1320

Query: 1321 RRCCPSELHYIASLSRCINWNAKGGKSKSFFAKTLDDRFIIKEIKRTEYESFMKFAPDYF 1380
             RCCPSELH+IASLSRCINWNAKGGKSKSFFAKTLDDRFIIKEIKRTEY+SFMKFAP+YF
Sbjct: 1321 SRCCPSELHFIASLSRCINWNAKGGKSKSFFAKTLDDRFIIKEIKRTEYDSFMKFAPEYF 1380

Query: 1381 KYINESFDMGNQTCLAKVLGIYQVTVREPKSGKEVRHELMVMENLSFGRNIIRQYDLKGA 1440
            KYINESFDMGNQTCLAKVLGIYQVTVRE KSGKE+RH+LMVMENLSFGRNIIRQYDLKGA
Sbjct: 1381 KYINESFDMGNQTCLAKVLGIYQVTVREQKSGKEMRHDLMVMENLSFGRNIIRQYDLKGA 1440

Query: 1441 LHARYNPATNDFGEVLLDQNFVNDMNSSPLYVNNQAKRRLQRAIWNDTAFLNSMNVMDYS 1500
            LHAR+NPATN+FGEVLLDQNFVNDMNSSPLYV+N+AKRRLQRAIWNDT FLNS+NVMDYS
Sbjct: 1441 LHARFNPATNNFGEVLLDQNFVNDMNSSPLYVSNRAKRRLQRAIWNDTTFLNSINVMDYS 1500

Query: 1501 LLVGVDAKKKELVCGIIDYLRQYTWDKQLETWAKSSLRPKNVLPTVVSPKEYKRRFRKFM 1523
            LLVGVDA+KKELVCGIIDYLRQYTWDKQLETW KSSL PKNVLPTVVSPKEYKRRFRKFM
Sbjct: 1501 LLVGVDAEKKELVCGIIDYLRQYTWDKQLETWVKSSLIPKNVLPTVVSPKEYKRRFRKFM 1560

BLAST of CmaCh09G011720 vs. ExPASy TrEMBL
Match: A0A0A0K755 (1-phosphatidylinositol-3-phosphate 5-kinase OS=Cucumis sativus OX=3659 GN=Csa_7G206930 PE=4 SV=1)

HSP 1 Score: 2260.3 bits (5856), Expect = 0.0e+00
Identity = 1169/1489 (78.51%), Postives = 1272/1489 (85.43%), Query Frame = 0

Query: 102  MSQNWSEVVHSNGQLSGRDDESVITISQEATHNGSGVRVNAEIEQSHSNWLDTDLWDPPE 161
            MS+N SEV HSN QLSGRDD SV++ SQE T N SGV VNA++EQS+SNW+D DLWDPPE
Sbjct: 1    MSRNSSEVAHSNDQLSGRDDGSVMSNSQETTQNASGVPVNADLEQSNSNWMDPDLWDPPE 60

Query: 162  PEDPQDHVEGGMGYDDDDDDEFDDSTEWNTSSSFSRSVDEASISYRIKEQKQRAMQQIMN 221
            PEDP+DH+EGGMGY+DDDD+EF D++EW+T+SSFSRSVDE S+SYR KE+KQRA+QQ+MN
Sbjct: 61   PEDPEDHMEGGMGYNDDDDEEFGDNSEWSTASSFSRSVDETSVSYRFKEEKQRALQQVMN 120

Query: 222  GKFKVFIGHLLKFVGVASSGEGSENWVDIVSSLSWEAATFLKPVVNGKAVDPEAHVKVKC 281
            GK K FI  LLK       GE SENWVDIVSSLSWEAATFLKPVVNGKA+DP+A+VKVKC
Sbjct: 121  GKHKAFIRDLLKL------GEDSENWVDIVSSLSWEAATFLKPVVNGKAMDPDANVKVKC 180

Query: 282  IATGTRSQSQFVKGMVFKKHAAHKHMPTHCKNPKLILIEGMLGEAPISRLSSFNSMDQEN 341
            IATGTR+QSQFVKGMVFKKHAAHKHMPTHCKNPKLILI+GMLGEAPISRLSSFNSMDQEN
Sbjct: 181  IATGTRNQSQFVKGMVFKKHAAHKHMPTHCKNPKLILIQGMLGEAPISRLSSFNSMDQEN 240

Query: 342  DFTKHVIEMIEVCTANVILVEKTTSRVIQEAMLKKGMTLVLDMKLHRLERIALCTGSPIL 401
            DFT HVIEMIE CTANVILVEKT +RVIQEA+LKKGMTLVLDMKLHRL+RIALCTGSPIL
Sbjct: 241  DFTNHVIEMIEGCTANVILVEKTAARVIQEAILKKGMTLVLDMKLHRLQRIALCTGSPIL 300

Query: 402  TSETLMSQNTRQCDAVYFEKIVEEHAGLLEGGKRPTKTLMFIEGCPTRLGCTILLKGAHS 461
            TSETLMSQ TRQCDAVYF+KIVEEHAG+LEGGK PTKTLMFIEGCPTRLGCTILLKGAHS
Sbjct: 301  TSETLMSQKTRQCDAVYFQKIVEEHAGVLEGGKMPTKTLMFIEGCPTRLGCTILLKGAHS 360

Query: 462  DELKKIKVVVQIAVVMAFHLMLETSFLVDQRAMFATIPFGGVPATVSSDPQSPLEPCSPN 521
            DELK++K+VVQ AVVMAFHL+LETSF+VDQRAMFATIPFGGVPAT SSD QSPLEP SPN
Sbjct: 361  DELKRVKIVVQCAVVMAFHLILETSFIVDQRAMFATIPFGGVPATASSDRQSPLEPRSPN 420

Query: 522  A-----------------------QGASNGSNL----------EGEPYNAAIFSGLSSIS 581
                                    +  S GSN+          E EPYN AIFSG SSIS
Sbjct: 421  VGKAESASDKSEQPTNICISRGSHEETSTGSNMELAEKLIMSSEPEPYNPAIFSGFSSIS 480

Query: 582  DSLKRVMGESFFLASPCQSFSSYFGHGKDLSGLVAKSDSIPSTPPEADQFDVEVRGSSDE 641
            DSLKRVMGESF  +SP QS SSYFGHG DLSGLVAKS SIPSTP    QFDVEV+GSSDE
Sbjct: 481  DSLKRVMGESFLSSSPYQSLSSYFGHGSDLSGLVAKSGSIPSTPQAVYQFDVEVKGSSDE 540

Query: 642  ENSIHEQFVPHQSTFDEGFGFHETAPNYSEDKLPKKTPFNSQSILVLMSSRNALKGTMCE 701
            ENS+HE  V HQST  EG GFHETA NYS D + KK   +SQSILVLMSSRNALKG +CE
Sbjct: 541  ENSVHESSVSHQSTL-EGLGFHETALNYSVDTMQKKMSLDSQSILVLMSSRNALKGAICE 600

Query: 702  QSHFSHIVFYKNFDAPLGKFLQENLLNQKNVCTVCGELPEAHYYYYAHSRKQLCIQVKQL 761
            QSHFSHIVFYK+FD PLGKFLQENLLNQ N+CTVCGELPEAH Y++AH RKQL IQVKQL
Sbjct: 601  QSHFSHIVFYKHFDVPLGKFLQENLLNQNNLCTVCGELPEAHIYFFAHYRKQLSIQVKQL 660

Query: 762  PVDKVLPGETEGKLWMWSRCSKCKSKGGPSKSTKRVLISTAARGLSFGKFLELCFSDCTL 821
            P DKVLPGETEGKLWMWSRCSKCKS GGPSKSTKRV ISTAARGLSFGKFLELCFSD TL
Sbjct: 661  PGDKVLPGETEGKLWMWSRCSKCKSTGGPSKSTKRVPISTAARGLSFGKFLELCFSDDTL 720

Query: 822  PSKSSVCGHSLFGDFLYFFGLGNMVAMFRYTEVAIYTVSMPPQKLEFNNSMRQGHLITET 881
             SKS VCGHSLFGDFLYFFGLGNMVAM RY+EVAIYTVSMPPQKLEFN+SMRQGH I ET
Sbjct: 721  LSKSPVCGHSLFGDFLYFFGLGNMVAMSRYSEVAIYTVSMPPQKLEFNSSMRQGHFIRET 780

Query: 882  ENVYEKGMILFTEIAQFLKKIISERKSSTV-------NDFSLLEEMLNEERSEFELNIQS 941
            ENVY KG++LFTEIA  LKKIISERKSS V       NDFS +EEMLNEERSEFE+NIQ+
Sbjct: 781  ENVYTKGVLLFTEIAHSLKKIISERKSSNVNHPQGFPNDFSFVEEMLNEERSEFEINIQN 840

Query: 942  SLTKKGNPDFPFHKYLSLNRLLWELLLESCIWDKRLQLLTSLGVTINSGTSENVEPEP-- 1001
            SLTKKGNPDF     L+LNRLLW+LLLESCIWD+RLQ L SLGVT NSG+SE VEPEP  
Sbjct: 841  SLTKKGNPDF-----LNLNRLLWDLLLESCIWDRRLQSLASLGVTTNSGSSEIVEPEPLL 900

Query: 1002 --------VRHEGLESIAQTDDRNVQQDVSVDENALHSKEIAVEHLDGESDGDELGLPSA 1061
                    V  E LESIA+ D+  VQQD+S+DEN L  KEI VE  DGES GDEL LPSA
Sbjct: 901  LKMNSNIDVGPEELESIAENDETKVQQDISLDENVLPLKEITVEGSDGESGGDELNLPSA 960

Query: 1062 TEVTEIPIMDDLSPKQLSRQGSLLNGFNRRHSDDEDPQAGRVLSSGD------IPSSTGN 1121
             EVTEIPI+DD SPK+LSRQG+L NGF+  HSD ED Q GRVLSSGD      IP S G+
Sbjct: 961  IEVTEIPIIDDPSPKKLSRQGTLSNGFDYHHSDYEDSQVGRVLSSGDLHIDRTIPISIGD 1020

Query: 1122 LTSDKLFWTPFSEIQQMRMRDIQRSYFPELKSISNYTPKLLPTAHEFINKEGQKLHIFLS 1181
             T  KLF TPFSEI+QM +RDIQRSYFPELKSIS+YTPKLLP A +FI++EGQKLHI L+
Sbjct: 1021 STLSKLFCTPFSEIRQMHLRDIQRSYFPELKSISSYTPKLLPAASDFIHEEGQKLHIHLA 1080

Query: 1182 NEKFIVSDYEGELSSIVACVLALLKDLSLETDLHNEDSKGEG------------LNKVPS 1241
            N+ F+VSDYEGELSSIVACVLALLKD   +TD HNEDSKGEG            LNKVPS
Sbjct: 1081 NDNFVVSDYEGELSSIVACVLALLKDQPFQTDSHNEDSKGEGGGALQPALSSRSLNKVPS 1140

Query: 1242 SGSSDSDSSVTSEEHQFSSFDRLNLLDSLLPKTFKRAEHEGVIKSLAKGKYLVNCPYFNQ 1301
            +GSSDSDSS++SEE++FSSFDRLNLLDSL+ +TFKR+++EGVIKSLAKGKYLVNCPY NQ
Sbjct: 1141 NGSSDSDSSISSEEYRFSSFDRLNLLDSLVSETFKRSDYEGVIKSLAKGKYLVNCPYINQ 1200

Query: 1302 FRDLRRRCCPSELHYIASLSRCINWNAKGGKSKSFFAKTLDDRFIIKEIKRTEYESFMKF 1361
            FRDLR RCCPSELH+IASLSRCINWNAKGGKSKSFFAKTLDDRFIIKEIKRTEY+SFMKF
Sbjct: 1201 FRDLRSRCCPSELHFIASLSRCINWNAKGGKSKSFFAKTLDDRFIIKEIKRTEYDSFMKF 1260

Query: 1362 APDYFKYINESFDMGNQTCLAKVLGIYQVTVREPKSGKEVRHELMVMENLSFGRNIIRQY 1421
            AP+YFKYINESFDMGNQTCLAKV+GIYQVTVREPKSGKE+RH+LMVMENLSFGRNIIRQY
Sbjct: 1261 APEYFKYINESFDMGNQTCLAKVVGIYQVTVREPKSGKEMRHDLMVMENLSFGRNIIRQY 1320

Query: 1422 DLKGALHARYNPATNDFGEVLLDQNFVNDMNSSPLYVNNQAKRRLQRAIWNDTAFLNSMN 1481
            DLKGALHAR+NPATN+FGEVLLDQNFVNDMNSSPLYV+N+A RRLQRAIWNDTAFLNS+N
Sbjct: 1321 DLKGALHARFNPATNNFGEVLLDQNFVNDMNSSPLYVSNRAMRRLQRAIWNDTAFLNSIN 1380

Query: 1482 VMDYSLLVGVDAKKKELVCGIIDYLRQYTWDKQLETWAKSSLRPKNVLPTVVSPKEYKRR 1523
            VMDYSLLVGVDA+KKELVCGIIDYLRQYTWDKQLETW KSSL PKNVLPTV+SPKEYKRR
Sbjct: 1381 VMDYSLLVGVDAEKKELVCGIIDYLRQYTWDKQLETWVKSSLIPKNVLPTVISPKEYKRR 1440

BLAST of CmaCh09G011720 vs. ExPASy TrEMBL
Match: A0A6J1F0I5 (1-phosphatidylinositol-3-phosphate 5-kinase OS=Cucurbita moschata OX=3662 GN=LOC111438379 PE=4 SV=1)

HSP 1 Score: 2235.3 bits (5791), Expect = 0.0e+00
Identity = 1169/1569 (74.51%), Postives = 1291/1569 (82.28%), Query Frame = 0

Query: 1    MCHYCGAVITDSEGKRLGNGNSFDLDDSGTVCICRVCKENQELEAMNLDNKSSSEALMLS 60
            MCH+CGAV+ DS  ++L NG+S  LDD+G +C C+VC ENQE E M  D+ SSSE+LMLS
Sbjct: 1    MCHHCGAVVADSAAQKLENGSSVKLDDAGAMCFCKVCNENQEQETMKRDDGSSSESLMLS 60

Query: 61   PMPSLSSCDSDCSTVANLDIKDGQEGTTRSSQDSIDCRQERMSQNWSEVVHSNGQLSGRD 120
            PMPS S+C+SDCST+ +LDI++GQEG+T SSQ+ ID +QER SQN S VVHSN QL GRD
Sbjct: 61   PMPSPSTCNSDCSTMGSLDIRNGQEGSTGSSQEDIDHKQERRSQNLSGVVHSNVQLGGRD 120

Query: 121  DESVITI-SQEATHNGSGVRVNAEIEQSHSNWLDTDLWDPPEPEDPQDHVEGGMGY---D 180
            DESV+ I +QEATHNG GV VNAE E+S+S+W+DT+LWDPPEPEDP DH+EGGMGY   D
Sbjct: 121  DESVMPINNQEATHNGFGVPVNAEFEKSNSSWMDTNLWDPPEPEDPDDHMEGGMGYYDDD 180

Query: 181  DDDDDEFDDSTEWNTSSSFSRSVDEASISYRIKEQKQRAMQQIMNGKFKVFIGHLLKFVG 240
            DDDDDEF DSTEW+ SSSFSRSVDEAS+S+R KE+KQRAMQ++MNGK+K FI HLL  VG
Sbjct: 181  DDDDDEFGDSTEWSMSSSFSRSVDEASVSHRSKEEKQRAMQEVMNGKYKAFIHHLLNLVG 240

Query: 241  VASSGEGSENWVDIVSSLSWEAATFLKPVVNGKAVDPEAHVKVKCIATGTRSQSQFVKGM 300
            V SSGE  ENW DIV SLSWEAATFLKP+VNGKA+DP AHVKVKCIA+GTRSQSQFVKGM
Sbjct: 241  VPSSGEDGENWADIVCSLSWEAATFLKPLVNGKAMDPGAHVKVKCIASGTRSQSQFVKGM 300

Query: 301  VFKKHAAHKHMPTHCKNPKLILIEGMLGEAPISRLSSFNSMDQENDFTKHVIEMIEVCTA 360
            VFKKHAAHKHM T+ KNP+LILI+G LGEAPIS LSSFNSMDQENDFT +VIEMIE CTA
Sbjct: 301  VFKKHAAHKHMVTNWKNPRLILIQGRLGEAPISGLSSFNSMDQENDFTNYVIEMIETCTA 360

Query: 361  NVILVEKTTSRVIQEAMLKKGMTLVLDMKLHRLERIALCTGSPILTSETLMSQNTRQCDA 420
            NVI+VEKT SRVIQEA+LK+ +TLVLDMKLHRLERIALCTGSPILTSETL SQ  RQCDA
Sbjct: 361  NVIIVEKTASRVIQEAILKRCITLVLDMKLHRLERIALCTGSPILTSETLASQKARQCDA 420

Query: 421  VYFEKIVEEHAGLLEGGKRPTKTLMFIEGCPTRLGCTILLKGAHSDELKKIKVVVQIAVV 480
            VYF+K+V EH+G LEGGKR TKTLMFIEGCP RLGCTILLKGA S ELK++K+VVQ AVV
Sbjct: 421  VYFQKVV-EHSGGLEGGKRSTKTLMFIEGCPARLGCTILLKGARS-ELKRVKLVVQRAVV 480

Query: 481  MAFHLMLETSFLVDQRAMFATIPFGGVPATVSSDPQSPLEPCSPNA-------------- 540
            MAFHL+LETSFLVDQRAMFATIPFGGV   VS DPQSPLEPCS N               
Sbjct: 481  MAFHLILETSFLVDQRAMFATIPFGGVSTMVSPDPQSPLEPCSSNILQVEKANDKSEPCV 540

Query: 541  -------------QGASNGSNL----------EGEPYNAAIFSGLSSISDSLKRVMGESF 600
                         + +SNGSNL          E EPYN AI SG SSISDS K+VMGESF
Sbjct: 541  SLLTDIYISSGPHEESSNGSNLKPVEKPIIPSELEPYNPAILSGFSSISDSSKKVMGESF 600

Query: 601  FLASPCQSFSSYFGHGKDLSGLVAKSDSIPSTPPEADQFDVEVRGSSDEENSIHEQFVPH 660
             L+S   S SSYFGH +D  GL  KSDSIPSTP  A++FDV+VRGSSDEENS HEQ   H
Sbjct: 601  LLSSTHHSLSSYFGHERDWIGLAPKSDSIPSTPQAANRFDVKVRGSSDEENSGHEQSASH 660

Query: 661  QSTFDEGFGFHETAPNYSEDKLPKKTPFNSQSILVLMSSRNALKGTMCEQSHFSHIVFYK 720
            QS   EG GFHE APN SE+ + KK+  +SQSILVLMSSRNALKGTMCEQSHF HIVFY+
Sbjct: 661  QSAL-EGLGFHENAPNCSEETMQKKSSLDSQSILVLMSSRNALKGTMCEQSHFLHIVFYR 720

Query: 721  NFDAPLGKFLQENLLNQKNVCTVCGELPEAHYYYYAHSRKQLCIQVKQLPVDKVLPGETE 780
            NFD PLGKFLQENLLNQ N CTVC ELPEAH+YY+AH  KQL IQV+QLP  K+LPG+TE
Sbjct: 721  NFDVPLGKFLQENLLNQTNFCTVCSELPEAHFYYFAHYGKQLSIQVQQLPGYKLLPGQTE 780

Query: 781  GKLWMWSRCSKCKSKGGPSKSTKRVLISTAARGLSFGKFLELCFSDCTLPSKSSVCGHSL 840
            GKLWMWSRCSKCKSKG PS STK VLISTAAR LSFGKFLELCFSD TLPSKSS CGHSL
Sbjct: 781  GKLWMWSRCSKCKSKGEPSTSTKMVLISTAARSLSFGKFLELCFSDETLPSKSSGCGHSL 840

Query: 841  FGDFLYFFGLGNMVAMFRYTEVAIYTVSMPPQKLEFNNSMRQGHLITETENVYEKGMILF 900
            FGDFLYFFGLGN VAMFRY+  AIYTVSMPPQKL+FN+SMRQGHLI ETENV +KG +LF
Sbjct: 841  FGDFLYFFGLGNRVAMFRYSAAAIYTVSMPPQKLDFNSSMRQGHLIRETENVKKKGKLLF 900

Query: 901  TEIAQFLKKIISERKSSTV------NDFSLLEEMLNEERSEFELNIQSSLTKKGNPDFPF 960
            TEIA  LKKI SE +SS V      NDFS +EEMLN+ERSEFE+ IQ+SL         F
Sbjct: 901  TEIAHSLKKISSECRSSNVKPQCFLNDFSSIEEMLNQERSEFEVKIQNSLA--------F 960

Query: 961  HKYLSLNRLLWELLLESCIWDKRLQLLTSLGVTINSGTSENVEPEPV----------RHE 1020
            HKYLSLN+LLWELLLESCIWD+RLQ LTSLG+T   GTSENVEPE V          RHE
Sbjct: 961  HKYLSLNQLLWELLLESCIWDRRLQSLTSLGLTSTPGTSENVEPESVTMNMNSNIDARHE 1020

Query: 1021 GLESIAQTDDRNVQQDVSVDENALHSKEIAVEHLDGESDGDELGLPSATEVTEIPIMDDL 1080
              ESIA  DD  VQQD+S DEN L  KEIAVE LDGES  DE   PS TEVTEI IMDDL
Sbjct: 1021 ESESIAANDDTEVQQDISWDENVLPMKEIAVEGLDGESGCDENHSPSVTEVTEIRIMDDL 1080

Query: 1081 SPKQLSRQGSLLNGFNRRHSDDEDPQAGRVLSSGD------IPSSTGNLTSDKLFWTPFS 1140
            SPK LSRQGSL NGFN+ H DDED Q  RV SSGD      IP  T + +  KL W P  
Sbjct: 1081 SPKHLSRQGSLSNGFNQHHLDDEDSQV-RVASSGDMHVDRTIPVLTADSSLGKLLWAPCV 1140

Query: 1141 EIQQMRMRDIQRSYFPELKSISNYTPKLLPTAHEFINKEGQKLHIFLSNEKFIVSDYEGE 1200
            EI+QM +RDIQRS+FPELKS S+YTPKL+P A  F N+EGQKLHI L+ + FIVSDYEGE
Sbjct: 1141 EIRQMHIRDIQRSFFPELKSFSSYTPKLIPAASAFTNEEGQKLHIRLAKDNFIVSDYEGE 1200

Query: 1201 LSSIVACVLALLKDLSLETDLHNEDSKGEG------------LNKVPSSGSSDSDSSVTS 1260
            LSSIVACVLALL+DLSL+TD HNEDSKGEG            LNKVPS+GSSDSDSS  S
Sbjct: 1201 LSSIVACVLALLEDLSLQTDSHNEDSKGEGGGVSQPNLSSQSLNKVPSNGSSDSDSSAAS 1260

Query: 1261 EEHQFSSFDRLNLLDSLLPKTFKRAEHEGVIKSLAKGKYLVNCPYFNQFRDLRRRCCPSE 1320
            + +QFS+F+RLNLLDSL+P+TFKR +HEGVIKSLA GKY+VNCPYFNQFRDLR +CCPSE
Sbjct: 1261 DAYQFSNFERLNLLDSLVPETFKRTDHEGVIKSLANGKYVVNCPYFNQFRDLRSQCCPSE 1320

Query: 1321 LHYIASLSRCINWNAKGGKSKSFFAKTLDDRFIIKEIKRTEYESFMKFAPDYFKYINESF 1380
            LHYIASLSRCI WNAKGGKS SFFAKTLDDRFIIKEIKRTEY+SFMKFAP+YFKYI +SF
Sbjct: 1321 LHYIASLSRCIKWNAKGGKSNSFFAKTLDDRFIIKEIKRTEYDSFMKFAPEYFKYIKQSF 1380

Query: 1381 DMGNQTCLAKVLGIYQVTVREPKSGKEVRHELMVMENLSFGRNIIRQYDLKGALHARYNP 1440
            DMGNQTCLAKVLGIYQV VREPKSGKE+RH++MVMENLSFGRNIIRQYDLKGALHARYNP
Sbjct: 1381 DMGNQTCLAKVLGIYQVAVREPKSGKEMRHDVMVMENLSFGRNIIRQYDLKGALHARYNP 1440

Query: 1441 ATNDFGEVLLDQNFVNDMNSSPLYVNNQAKRRLQRAIWNDTAFLNSMNVMDYSLLVGVDA 1495
            A N+FGEVLLDQNFV DMNSSP+YV+N+AKRR+QRAIWNDTAFLNS+NVMDYSLLVGVDA
Sbjct: 1441 AANNFGEVLLDQNFVKDMNSSPIYVSNRAKRRMQRAIWNDTAFLNSINVMDYSLLVGVDA 1500

BLAST of CmaCh09G011720 vs. NCBI nr
Match: XP_022976897.1 (putative 1-phosphatidylinositol-3-phosphate 5-kinase FAB1D [Cucurbita maxima] >XP_022976898.1 putative 1-phosphatidylinositol-3-phosphate 5-kinase FAB1D [Cucurbita maxima])

HSP 1 Score: 3038.8 bits (7877), Expect = 0.0e+00
Identity = 1522/1522 (100.00%), Postives = 1522/1522 (100.00%), Query Frame = 0

Query: 1    MCHYCGAVITDSEGKRLGNGNSFDLDDSGTVCICRVCKENQELEAMNLDNKSSSEALMLS 60
            MCHYCGAVITDSEGKRLGNGNSFDLDDSGTVCICRVCKENQELEAMNLDNKSSSEALMLS
Sbjct: 1    MCHYCGAVITDSEGKRLGNGNSFDLDDSGTVCICRVCKENQELEAMNLDNKSSSEALMLS 60

Query: 61   PMPSLSSCDSDCSTVANLDIKDGQEGTTRSSQDSIDCRQERMSQNWSEVVHSNGQLSGRD 120
            PMPSLSSCDSDCSTVANLDIKDGQEGTTRSSQDSIDCRQERMSQNWSEVVHSNGQLSGRD
Sbjct: 61   PMPSLSSCDSDCSTVANLDIKDGQEGTTRSSQDSIDCRQERMSQNWSEVVHSNGQLSGRD 120

Query: 121  DESVITISQEATHNGSGVRVNAEIEQSHSNWLDTDLWDPPEPEDPQDHVEGGMGYDDDDD 180
            DESVITISQEATHNGSGVRVNAEIEQSHSNWLDTDLWDPPEPEDPQDHVEGGMGYDDDDD
Sbjct: 121  DESVITISQEATHNGSGVRVNAEIEQSHSNWLDTDLWDPPEPEDPQDHVEGGMGYDDDDD 180

Query: 181  DEFDDSTEWNTSSSFSRSVDEASISYRIKEQKQRAMQQIMNGKFKVFIGHLLKFVGVASS 240
            DEFDDSTEWNTSSSFSRSVDEASISYRIKEQKQRAMQQIMNGKFKVFIGHLLKFVGVASS
Sbjct: 181  DEFDDSTEWNTSSSFSRSVDEASISYRIKEQKQRAMQQIMNGKFKVFIGHLLKFVGVASS 240

Query: 241  GEGSENWVDIVSSLSWEAATFLKPVVNGKAVDPEAHVKVKCIATGTRSQSQFVKGMVFKK 300
            GEGSENWVDIVSSLSWEAATFLKPVVNGKAVDPEAHVKVKCIATGTRSQSQFVKGMVFKK
Sbjct: 241  GEGSENWVDIVSSLSWEAATFLKPVVNGKAVDPEAHVKVKCIATGTRSQSQFVKGMVFKK 300

Query: 301  HAAHKHMPTHCKNPKLILIEGMLGEAPISRLSSFNSMDQENDFTKHVIEMIEVCTANVIL 360
            HAAHKHMPTHCKNPKLILIEGMLGEAPISRLSSFNSMDQENDFTKHVIEMIEVCTANVIL
Sbjct: 301  HAAHKHMPTHCKNPKLILIEGMLGEAPISRLSSFNSMDQENDFTKHVIEMIEVCTANVIL 360

Query: 361  VEKTTSRVIQEAMLKKGMTLVLDMKLHRLERIALCTGSPILTSETLMSQNTRQCDAVYFE 420
            VEKTTSRVIQEAMLKKGMTLVLDMKLHRLERIALCTGSPILTSETLMSQNTRQCDAVYFE
Sbjct: 361  VEKTTSRVIQEAMLKKGMTLVLDMKLHRLERIALCTGSPILTSETLMSQNTRQCDAVYFE 420

Query: 421  KIVEEHAGLLEGGKRPTKTLMFIEGCPTRLGCTILLKGAHSDELKKIKVVVQIAVVMAFH 480
            KIVEEHAGLLEGGKRPTKTLMFIEGCPTRLGCTILLKGAHSDELKKIKVVVQIAVVMAFH
Sbjct: 421  KIVEEHAGLLEGGKRPTKTLMFIEGCPTRLGCTILLKGAHSDELKKIKVVVQIAVVMAFH 480

Query: 481  LMLETSFLVDQRAMFATIPFGGVPATVSSDPQSPLEPCSPNAQGASNGSNLEGEPYNAAI 540
            LMLETSFLVDQRAMFATIPFGGVPATVSSDPQSPLEPCSPNAQGASNGSNLEGEPYNAAI
Sbjct: 481  LMLETSFLVDQRAMFATIPFGGVPATVSSDPQSPLEPCSPNAQGASNGSNLEGEPYNAAI 540

Query: 541  FSGLSSISDSLKRVMGESFFLASPCQSFSSYFGHGKDLSGLVAKSDSIPSTPPEADQFDV 600
            FSGLSSISDSLKRVMGESFFLASPCQSFSSYFGHGKDLSGLVAKSDSIPSTPPEADQFDV
Sbjct: 541  FSGLSSISDSLKRVMGESFFLASPCQSFSSYFGHGKDLSGLVAKSDSIPSTPPEADQFDV 600

Query: 601  EVRGSSDEENSIHEQFVPHQSTFDEGFGFHETAPNYSEDKLPKKTPFNSQSILVLMSSRN 660
            EVRGSSDEENSIHEQFVPHQSTFDEGFGFHETAPNYSEDKLPKKTPFNSQSILVLMSSRN
Sbjct: 601  EVRGSSDEENSIHEQFVPHQSTFDEGFGFHETAPNYSEDKLPKKTPFNSQSILVLMSSRN 660

Query: 661  ALKGTMCEQSHFSHIVFYKNFDAPLGKFLQENLLNQKNVCTVCGELPEAHYYYYAHSRKQ 720
            ALKGTMCEQSHFSHIVFYKNFDAPLGKFLQENLLNQKNVCTVCGELPEAHYYYYAHSRKQ
Sbjct: 661  ALKGTMCEQSHFSHIVFYKNFDAPLGKFLQENLLNQKNVCTVCGELPEAHYYYYAHSRKQ 720

Query: 721  LCIQVKQLPVDKVLPGETEGKLWMWSRCSKCKSKGGPSKSTKRVLISTAARGLSFGKFLE 780
            LCIQVKQLPVDKVLPGETEGKLWMWSRCSKCKSKGGPSKSTKRVLISTAARGLSFGKFLE
Sbjct: 721  LCIQVKQLPVDKVLPGETEGKLWMWSRCSKCKSKGGPSKSTKRVLISTAARGLSFGKFLE 780

Query: 781  LCFSDCTLPSKSSVCGHSLFGDFLYFFGLGNMVAMFRYTEVAIYTVSMPPQKLEFNNSMR 840
            LCFSDCTLPSKSSVCGHSLFGDFLYFFGLGNMVAMFRYTEVAIYTVSMPPQKLEFNNSMR
Sbjct: 781  LCFSDCTLPSKSSVCGHSLFGDFLYFFGLGNMVAMFRYTEVAIYTVSMPPQKLEFNNSMR 840

Query: 841  QGHLITETENVYEKGMILFTEIAQFLKKIISERKSSTVNDFSLLEEMLNEERSEFELNIQ 900
            QGHLITETENVYEKGMILFTEIAQFLKKIISERKSSTVNDFSLLEEMLNEERSEFELNIQ
Sbjct: 841  QGHLITETENVYEKGMILFTEIAQFLKKIISERKSSTVNDFSLLEEMLNEERSEFELNIQ 900

Query: 901  SSLTKKGNPDFPFHKYLSLNRLLWELLLESCIWDKRLQLLTSLGVTINSGTSENVEPEPV 960
            SSLTKKGNPDFPFHKYLSLNRLLWELLLESCIWDKRLQLLTSLGVTINSGTSENVEPEPV
Sbjct: 901  SSLTKKGNPDFPFHKYLSLNRLLWELLLESCIWDKRLQLLTSLGVTINSGTSENVEPEPV 960

Query: 961  RHEGLESIAQTDDRNVQQDVSVDENALHSKEIAVEHLDGESDGDELGLPSATEVTEIPIM 1020
            RHEGLESIAQTDDRNVQQDVSVDENALHSKEIAVEHLDGESDGDELGLPSATEVTEIPIM
Sbjct: 961  RHEGLESIAQTDDRNVQQDVSVDENALHSKEIAVEHLDGESDGDELGLPSATEVTEIPIM 1020

Query: 1021 DDLSPKQLSRQGSLLNGFNRRHSDDEDPQAGRVLSSGDIPSSTGNLTSDKLFWTPFSEIQ 1080
            DDLSPKQLSRQGSLLNGFNRRHSDDEDPQAGRVLSSGDIPSSTGNLTSDKLFWTPFSEIQ
Sbjct: 1021 DDLSPKQLSRQGSLLNGFNRRHSDDEDPQAGRVLSSGDIPSSTGNLTSDKLFWTPFSEIQ 1080

Query: 1081 QMRMRDIQRSYFPELKSISNYTPKLLPTAHEFINKEGQKLHIFLSNEKFIVSDYEGELSS 1140
            QMRMRDIQRSYFPELKSISNYTPKLLPTAHEFINKEGQKLHIFLSNEKFIVSDYEGELSS
Sbjct: 1081 QMRMRDIQRSYFPELKSISNYTPKLLPTAHEFINKEGQKLHIFLSNEKFIVSDYEGELSS 1140

Query: 1141 IVACVLALLKDLSLETDLHNEDSKGEGLNKVPSSGSSDSDSSVTSEEHQFSSFDRLNLLD 1200
            IVACVLALLKDLSLETDLHNEDSKGEGLNKVPSSGSSDSDSSVTSEEHQFSSFDRLNLLD
Sbjct: 1141 IVACVLALLKDLSLETDLHNEDSKGEGLNKVPSSGSSDSDSSVTSEEHQFSSFDRLNLLD 1200

Query: 1201 SLLPKTFKRAEHEGVIKSLAKGKYLVNCPYFNQFRDLRRRCCPSELHYIASLSRCINWNA 1260
            SLLPKTFKRAEHEGVIKSLAKGKYLVNCPYFNQFRDLRRRCCPSELHYIASLSRCINWNA
Sbjct: 1201 SLLPKTFKRAEHEGVIKSLAKGKYLVNCPYFNQFRDLRRRCCPSELHYIASLSRCINWNA 1260

Query: 1261 KGGKSKSFFAKTLDDRFIIKEIKRTEYESFMKFAPDYFKYINESFDMGNQTCLAKVLGIY 1320
            KGGKSKSFFAKTLDDRFIIKEIKRTEYESFMKFAPDYFKYINESFDMGNQTCLAKVLGIY
Sbjct: 1261 KGGKSKSFFAKTLDDRFIIKEIKRTEYESFMKFAPDYFKYINESFDMGNQTCLAKVLGIY 1320

Query: 1321 QVTVREPKSGKEVRHELMVMENLSFGRNIIRQYDLKGALHARYNPATNDFGEVLLDQNFV 1380
            QVTVREPKSGKEVRHELMVMENLSFGRNIIRQYDLKGALHARYNPATNDFGEVLLDQNFV
Sbjct: 1321 QVTVREPKSGKEVRHELMVMENLSFGRNIIRQYDLKGALHARYNPATNDFGEVLLDQNFV 1380

Query: 1381 NDMNSSPLYVNNQAKRRLQRAIWNDTAFLNSMNVMDYSLLVGVDAKKKELVCGIIDYLRQ 1440
            NDMNSSPLYVNNQAKRRLQRAIWNDTAFLNSMNVMDYSLLVGVDAKKKELVCGIIDYLRQ
Sbjct: 1381 NDMNSSPLYVNNQAKRRLQRAIWNDTAFLNSMNVMDYSLLVGVDAKKKELVCGIIDYLRQ 1440

Query: 1441 YTWDKQLETWAKSSLRPKNVLPTVVSPKEYKRRFRKFMSAHFLSVPDNWCQQQNLHGPWG 1500
            YTWDKQLETWAKSSLRPKNVLPTVVSPKEYKRRFRKFMSAHFLSVPDNWCQQQNLHGPWG
Sbjct: 1441 YTWDKQLETWAKSSLRPKNVLPTVVSPKEYKRRFRKFMSAHFLSVPDNWCQQQNLHGPWG 1500

Query: 1501 ACGGTIDHASSQTNAEENDLSS 1523
            ACGGTIDHASSQTNAEENDLSS
Sbjct: 1501 ACGGTIDHASSQTNAEENDLSS 1522

BLAST of CmaCh09G011720 vs. NCBI nr
Match: XP_022932526.1 (putative 1-phosphatidylinositol-3-phosphate 5-kinase FAB1D [Cucurbita moschata])

HSP 1 Score: 2989.5 bits (7749), Expect = 0.0e+00
Identity = 1494/1522 (98.16%), Postives = 1510/1522 (99.21%), Query Frame = 0

Query: 1    MCHYCGAVITDSEGKRLGNGNSFDLDDSGTVCICRVCKENQELEAMNLDNKSSSEALMLS 60
            MCHYCGAVITDSEGKRLGNGNSFDLDDSGTVCICRVCKENQEL+ MNLDNKSSSEALMLS
Sbjct: 1    MCHYCGAVITDSEGKRLGNGNSFDLDDSGTVCICRVCKENQELKTMNLDNKSSSEALMLS 60

Query: 61   PMPSLSSCDSDCSTVANLDIKDGQEGTTRSSQDSIDCRQERMSQNWSEVVHSNGQLSGRD 120
            PMPSLSSCDSDCSTVANLD+KDGQ+GTTRSSQDSIDCRQERMSQNWSEVVHSNGQLSGRD
Sbjct: 61   PMPSLSSCDSDCSTVANLDVKDGQDGTTRSSQDSIDCRQERMSQNWSEVVHSNGQLSGRD 120

Query: 121  DESVITISQEATHNGSGVRVNAEIEQSHSNWLDTDLWDPPEPEDPQDHVEGGMGYDDDDD 180
            DESVITISQEATHNGSGVRVNAEIEQSHSNWLDTDLWDPPEPEDPQDH+EGGMGYDDDDD
Sbjct: 121  DESVITISQEATHNGSGVRVNAEIEQSHSNWLDTDLWDPPEPEDPQDHMEGGMGYDDDDD 180

Query: 181  DEFDDSTEWNTSSSFSRSVDEASISYRIKEQKQRAMQQIMNGKFKVFIGHLLKFVGVASS 240
            DEFDDSTEWNTSSSFSRSVDEAS+SYRIKEQKQRAMQQIMNGKFKVFIGHLLKFVGVASS
Sbjct: 181  DEFDDSTEWNTSSSFSRSVDEASVSYRIKEQKQRAMQQIMNGKFKVFIGHLLKFVGVASS 240

Query: 241  GEGSENWVDIVSSLSWEAATFLKPVVNGKAVDPEAHVKVKCIATGTRSQSQFVKGMVFKK 300
            GE SENWVDIVSSLSWEAATFLKPVVNGKAVDPEAHVKVKCIATGTRSQSQFVKGMVFKK
Sbjct: 241  GEDSENWVDIVSSLSWEAATFLKPVVNGKAVDPEAHVKVKCIATGTRSQSQFVKGMVFKK 300

Query: 301  HAAHKHMPTHCKNPKLILIEGMLGEAPISRLSSFNSMDQENDFTKHVIEMIEVCTANVIL 360
            HAAHKHMPTHCKNPKLILIEGMLGEAPISRLSSFNSMDQENDFTKHVIEMIEVCTANVIL
Sbjct: 301  HAAHKHMPTHCKNPKLILIEGMLGEAPISRLSSFNSMDQENDFTKHVIEMIEVCTANVIL 360

Query: 361  VEKTTSRVIQEAMLKKGMTLVLDMKLHRLERIALCTGSPILTSETLMSQNTRQCDAVYFE 420
            VEKTTSRVIQEAMLKKGMTLVLDMKLHRLERIALCTGSPILTSETLMSQNTRQCDAVYFE
Sbjct: 361  VEKTTSRVIQEAMLKKGMTLVLDMKLHRLERIALCTGSPILTSETLMSQNTRQCDAVYFE 420

Query: 421  KIVEEHAGLLEGGKRPTKTLMFIEGCPTRLGCTILLKGAHSDELKKIKVVVQIAVVMAFH 480
            KIVEEHAGLLEGGKRPTKTLMFIEGCPTRLGCTILLKGAHSDELKKIKVVVQIAVVMAFH
Sbjct: 421  KIVEEHAGLLEGGKRPTKTLMFIEGCPTRLGCTILLKGAHSDELKKIKVVVQIAVVMAFH 480

Query: 481  LMLETSFLVDQRAMFATIPFGGVPATVSSDPQSPLEPCSPNAQGASNGSNLEGEPYNAAI 540
            LMLETSFLVDQRAMFATIPFGGVPATVSSDPQSPLEPCSPNAQGASNGSNLEGEPYN AI
Sbjct: 481  LMLETSFLVDQRAMFATIPFGGVPATVSSDPQSPLEPCSPNAQGASNGSNLEGEPYNPAI 540

Query: 541  FSGLSSISDSLKRVMGESFFLASPCQSFSSYFGHGKDLSGLVAKSDSIPSTPPEADQFDV 600
            FSGLSSISDSLKRVMGESFFLASPCQSFSSYFGHGKDLSGLVAKSDSIPSTPPEADQFDV
Sbjct: 541  FSGLSSISDSLKRVMGESFFLASPCQSFSSYFGHGKDLSGLVAKSDSIPSTPPEADQFDV 600

Query: 601  EVRGSSDEENSIHEQFVPHQSTFDEGFGFHETAPNYSEDKLPKKTPFNSQSILVLMSSRN 660
            EVRGSSDEENSIHEQFVPHQSTFDEGFGFHETAPNYSEDKLP+KTPFNSQSILVLMSSRN
Sbjct: 601  EVRGSSDEENSIHEQFVPHQSTFDEGFGFHETAPNYSEDKLPEKTPFNSQSILVLMSSRN 660

Query: 661  ALKGTMCEQSHFSHIVFYKNFDAPLGKFLQENLLNQKNVCTVCGELPEAHYYYYAHSRKQ 720
            A KGTMCEQSHFSHIVFYKNFDAPLGKFLQENLLNQKNVCTVCGELPEAHYYYYAHSRKQ
Sbjct: 661  ASKGTMCEQSHFSHIVFYKNFDAPLGKFLQENLLNQKNVCTVCGELPEAHYYYYAHSRKQ 720

Query: 721  LCIQVKQLPVDKVLPGETEGKLWMWSRCSKCKSKGGPSKSTKRVLISTAARGLSFGKFLE 780
            LCIQVKQLPV+KVLPGETEGKLWMWSRCSKCKSKGGPSKSTKRVLISTAARGLSFGKFLE
Sbjct: 721  LCIQVKQLPVNKVLPGETEGKLWMWSRCSKCKSKGGPSKSTKRVLISTAARGLSFGKFLE 780

Query: 781  LCFSDCTLPSKSSVCGHSLFGDFLYFFGLGNMVAMFRYTEVAIYTVSMPPQKLEFNNSMR 840
            LCFSDCTLPSKSSVCGHSLFGDFLYFFGLGNMVAMFRYTEVAIYTVSMPPQKLEFN+SMR
Sbjct: 781  LCFSDCTLPSKSSVCGHSLFGDFLYFFGLGNMVAMFRYTEVAIYTVSMPPQKLEFNSSMR 840

Query: 841  QGHLITETENVYEKGMILFTEIAQFLKKIISERKSSTVNDFSLLEEMLNEERSEFELNIQ 900
            QGHLITETENVY KGMILFTEIAQFLKKIISERKSSTVNDFSLLEEMLNEERSEFELNIQ
Sbjct: 841  QGHLITETENVYAKGMILFTEIAQFLKKIISERKSSTVNDFSLLEEMLNEERSEFELNIQ 900

Query: 901  SSLTKKGNPDFPFHKYLSLNRLLWELLLESCIWDKRLQLLTSLGVTINSGTSENVEPEPV 960
            SSLTKKGNPDFPFHKYLSLNRLLWELLLESCIWDKRLQLLTS+GVT+NSGTSENVEPEPV
Sbjct: 901  SSLTKKGNPDFPFHKYLSLNRLLWELLLESCIWDKRLQLLTSVGVTLNSGTSENVEPEPV 960

Query: 961  RHEGLESIAQTDDRNVQQDVSVDENALHSKEIAVEHLDGESDGDELGLPSATEVTEIPIM 1020
              EGLESIAQTDDRNVQQDVSVDENALH KEIAVEHLDGESDGDELGLPSATEVTEIPIM
Sbjct: 961  GQEGLESIAQTDDRNVQQDVSVDENALHPKEIAVEHLDGESDGDELGLPSATEVTEIPIM 1020

Query: 1021 DDLSPKQLSRQGSLLNGFNRRHSDDEDPQAGRVLSSGDIPSSTGNLTSDKLFWTPFSEIQ 1080
            DDLSPKQLSRQGSL NGFNRRHSDDEDPQA RVLSSGDIPSSTGNLT DKLFWTPFSEIQ
Sbjct: 1021 DDLSPKQLSRQGSLSNGFNRRHSDDEDPQAVRVLSSGDIPSSTGNLTLDKLFWTPFSEIQ 1080

Query: 1081 QMRMRDIQRSYFPELKSISNYTPKLLPTAHEFINKEGQKLHIFLSNEKFIVSDYEGELSS 1140
            QMRMRDIQRSYFPELKS+SNYTPKLLPTAH+FINKEGQKLHIFLSNEKFIVSDYEGELSS
Sbjct: 1081 QMRMRDIQRSYFPELKSVSNYTPKLLPTAHDFINKEGQKLHIFLSNEKFIVSDYEGELSS 1140

Query: 1141 IVACVLALLKDLSLETDLHNEDSKGEGLNKVPSSGSSDSDSSVTSEEHQFSSFDRLNLLD 1200
            IVACVLALLKDLSLETDLHNEDSKGEGLNKVPS+GSSDSDSSV+SEEHQFSSFDRLNLLD
Sbjct: 1141 IVACVLALLKDLSLETDLHNEDSKGEGLNKVPSNGSSDSDSSVSSEEHQFSSFDRLNLLD 1200

Query: 1201 SLLPKTFKRAEHEGVIKSLAKGKYLVNCPYFNQFRDLRRRCCPSELHYIASLSRCINWNA 1260
            SLLPKTFKRAEHEGVIKSLAKGKYLVNCPYFNQFRDLRRRCCPSELHYIASLSRCI+WNA
Sbjct: 1201 SLLPKTFKRAEHEGVIKSLAKGKYLVNCPYFNQFRDLRRRCCPSELHYIASLSRCIHWNA 1260

Query: 1261 KGGKSKSFFAKTLDDRFIIKEIKRTEYESFMKFAPDYFKYINESFDMGNQTCLAKVLGIY 1320
            KGGKSKSFFAKTLDDRFIIKEIKRTEYESFMKFAPDYFKYINESFDMGNQTCLAKVLGIY
Sbjct: 1261 KGGKSKSFFAKTLDDRFIIKEIKRTEYESFMKFAPDYFKYINESFDMGNQTCLAKVLGIY 1320

Query: 1321 QVTVREPKSGKEVRHELMVMENLSFGRNIIRQYDLKGALHARYNPATNDFGEVLLDQNFV 1380
            QVTVREPKSGKEVRHELMVMENLSFGRNIIRQYDLKGALHARYNPATNDFGEVLLDQNFV
Sbjct: 1321 QVTVREPKSGKEVRHELMVMENLSFGRNIIRQYDLKGALHARYNPATNDFGEVLLDQNFV 1380

Query: 1381 NDMNSSPLYVNNQAKRRLQRAIWNDTAFLNSMNVMDYSLLVGVDAKKKELVCGIIDYLRQ 1440
            NDMNSSPLYVNNQAKRRLQRAIWNDTAFLNSMNVMDYSLLVGVDA+KKELVCGIIDYLRQ
Sbjct: 1381 NDMNSSPLYVNNQAKRRLQRAIWNDTAFLNSMNVMDYSLLVGVDAEKKELVCGIIDYLRQ 1440

Query: 1441 YTWDKQLETWAKSSLRPKNVLPTVVSPKEYKRRFRKFMSAHFLSVPDNWCQQQNLHGPWG 1500
            YTWDKQLETWAKSSLRPKNVLPTVVSPKEYKRRFRKFMSAHFLSVPDNWCQQQNLHGPWG
Sbjct: 1441 YTWDKQLETWAKSSLRPKNVLPTVVSPKEYKRRFRKFMSAHFLSVPDNWCQQQNLHGPWG 1500

Query: 1501 ACGGTIDHASSQTNAEENDLSS 1523
            ACGGTID ASSQTNAEENDLSS
Sbjct: 1501 ACGGTIDRASSQTNAEENDLSS 1522

BLAST of CmaCh09G011720 vs. NCBI nr
Match: KAG7025096.1 (putative 1-phosphatidylinositol-3-phosphate 5-kinase FAB1D [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 2985.7 bits (7739), Expect = 0.0e+00
Identity = 1494/1523 (98.10%), Postives = 1510/1523 (99.15%), Query Frame = 0

Query: 1    MCHYCGAVITDSEGKRLGNGNSFDLDDSGTVCICRVCKENQELEAMNLDNKSSSEALMLS 60
            MCHYCGAVITDSEGKRLGNGNSFDLDDSGTVCIC+VCKENQEL+ MNLDNKSSSEALMLS
Sbjct: 1    MCHYCGAVITDSEGKRLGNGNSFDLDDSGTVCICKVCKENQELKTMNLDNKSSSEALMLS 60

Query: 61   PMPSLSSCDSDCSTVANLDIKDGQEGTTRSSQDSIDCRQERMSQNWSEVVHSNGQLSGRD 120
            PMPSLSSCDSDCSTVANLD+KDGQ+GTTRSSQDSIDCRQERMSQNWSEVVHSNGQLSGRD
Sbjct: 61   PMPSLSSCDSDCSTVANLDVKDGQDGTTRSSQDSIDCRQERMSQNWSEVVHSNGQLSGRD 120

Query: 121  DESVITISQEATHNGSGVRVNAEIEQSHSNWLDTDLWDPPEPEDPQDHVEGGMGY-DDDD 180
            DESVITISQEATHNGSGVRVNAEIEQSHSNWLDTDLWDPPEPEDPQDHVEGGMGY DDDD
Sbjct: 121  DESVITISQEATHNGSGVRVNAEIEQSHSNWLDTDLWDPPEPEDPQDHVEGGMGYDDDDD 180

Query: 181  DDEFDDSTEWNTSSSFSRSVDEASISYRIKEQKQRAMQQIMNGKFKVFIGHLLKFVGVAS 240
            DDEFDDSTEWNTSSSFSRSVDEAS+SYRIKEQKQRAMQQIMNGKFKVFIGHLLKFVGVAS
Sbjct: 181  DDEFDDSTEWNTSSSFSRSVDEASVSYRIKEQKQRAMQQIMNGKFKVFIGHLLKFVGVAS 240

Query: 241  SGEGSENWVDIVSSLSWEAATFLKPVVNGKAVDPEAHVKVKCIATGTRSQSQFVKGMVFK 300
            SGE SENWVDIVSSLSWEAATFLKPVVNGKAVDPEAHVKVKCIATGTRSQSQFVKGMVFK
Sbjct: 241  SGEDSENWVDIVSSLSWEAATFLKPVVNGKAVDPEAHVKVKCIATGTRSQSQFVKGMVFK 300

Query: 301  KHAAHKHMPTHCKNPKLILIEGMLGEAPISRLSSFNSMDQENDFTKHVIEMIEVCTANVI 360
            KHAAHKHMPTHCKNPKLILIEGMLGEAPISRLSSFNSMDQENDFTKHVIEMIEVCTANVI
Sbjct: 301  KHAAHKHMPTHCKNPKLILIEGMLGEAPISRLSSFNSMDQENDFTKHVIEMIEVCTANVI 360

Query: 361  LVEKTTSRVIQEAMLKKGMTLVLDMKLHRLERIALCTGSPILTSETLMSQNTRQCDAVYF 420
            LVEKTTSRVIQEAMLKKGMTLVLDMKLHRLERIALCTGSPILTSETLMSQNTRQCDAVYF
Sbjct: 361  LVEKTTSRVIQEAMLKKGMTLVLDMKLHRLERIALCTGSPILTSETLMSQNTRQCDAVYF 420

Query: 421  EKIVEEHAGLLEGGKRPTKTLMFIEGCPTRLGCTILLKGAHSDELKKIKVVVQIAVVMAF 480
            EKIVEEHAGLLEGGKRPTKTLMFIEGCPTRLGCTILLKGAHSDELKKIKVVVQIAVVMAF
Sbjct: 421  EKIVEEHAGLLEGGKRPTKTLMFIEGCPTRLGCTILLKGAHSDELKKIKVVVQIAVVMAF 480

Query: 481  HLMLETSFLVDQRAMFATIPFGGVPATVSSDPQSPLEPCSPNAQGASNGSNLEGEPYNAA 540
            HLMLETSFLVDQRAMFATIPFGGVPATVSSDPQSPLEPCSPNAQGAS GSNLEGEPYN A
Sbjct: 481  HLMLETSFLVDQRAMFATIPFGGVPATVSSDPQSPLEPCSPNAQGASYGSNLEGEPYNPA 540

Query: 541  IFSGLSSISDSLKRVMGESFFLASPCQSFSSYFGHGKDLSGLVAKSDSIPSTPPEADQFD 600
            IFSGLSSISDSLKRVMGESFFLASPCQSFSSYFGHGKDLSGLVAKSDSIPSTPPEADQFD
Sbjct: 541  IFSGLSSISDSLKRVMGESFFLASPCQSFSSYFGHGKDLSGLVAKSDSIPSTPPEADQFD 600

Query: 601  VEVRGSSDEENSIHEQFVPHQSTFDEGFGFHETAPNYSEDKLPKKTPFNSQSILVLMSSR 660
            VEVRGSSDEENSIHEQFVPHQSTFDEGFGFHETAPNYSEDKLP+KTPFNSQSILVLMSSR
Sbjct: 601  VEVRGSSDEENSIHEQFVPHQSTFDEGFGFHETAPNYSEDKLPEKTPFNSQSILVLMSSR 660

Query: 661  NALKGTMCEQSHFSHIVFYKNFDAPLGKFLQENLLNQKNVCTVCGELPEAHYYYYAHSRK 720
            NA KGTMCEQSHFSHIVFYKNFDAPLGKFLQENLLNQKNVCTVCGELPEAHYYYYAHSRK
Sbjct: 661  NASKGTMCEQSHFSHIVFYKNFDAPLGKFLQENLLNQKNVCTVCGELPEAHYYYYAHSRK 720

Query: 721  QLCIQVKQLPVDKVLPGETEGKLWMWSRCSKCKSKGGPSKSTKRVLISTAARGLSFGKFL 780
            QLCIQVKQLPV+KVLPGETEGKLWMWSRCSKCKSKGGPSKSTKRVLISTAARGLSFGKFL
Sbjct: 721  QLCIQVKQLPVNKVLPGETEGKLWMWSRCSKCKSKGGPSKSTKRVLISTAARGLSFGKFL 780

Query: 781  ELCFSDCTLPSKSSVCGHSLFGDFLYFFGLGNMVAMFRYTEVAIYTVSMPPQKLEFNNSM 840
            ELCFSDCTLPSKSSVCGHSLFGDFLYFFGLGNMVAMFRYTEVAIYTVSMPPQKLEFN+SM
Sbjct: 781  ELCFSDCTLPSKSSVCGHSLFGDFLYFFGLGNMVAMFRYTEVAIYTVSMPPQKLEFNSSM 840

Query: 841  RQGHLITETENVYEKGMILFTEIAQFLKKIISERKSSTVNDFSLLEEMLNEERSEFELNI 900
            RQGHLITETENVY KGMILFTEIAQFLKKIISERKSSTVNDFSLLEEMLNEERSEFELNI
Sbjct: 841  RQGHLITETENVYAKGMILFTEIAQFLKKIISERKSSTVNDFSLLEEMLNEERSEFELNI 900

Query: 901  QSSLTKKGNPDFPFHKYLSLNRLLWELLLESCIWDKRLQLLTSLGVTINSGTSENVEPEP 960
            QSSLTKKGNPDFPFHKYLSLNRLLWELLLESCIWDKRLQLLTS+GVT+NSGTSENVEPEP
Sbjct: 901  QSSLTKKGNPDFPFHKYLSLNRLLWELLLESCIWDKRLQLLTSVGVTLNSGTSENVEPEP 960

Query: 961  VRHEGLESIAQTDDRNVQQDVSVDENALHSKEIAVEHLDGESDGDELGLPSATEVTEIPI 1020
            V HEGLESIAQTDDRNVQQDVSVDENALH KEIAVEHLDGESDGDELGLPSATEVTEIPI
Sbjct: 961  VGHEGLESIAQTDDRNVQQDVSVDENALHPKEIAVEHLDGESDGDELGLPSATEVTEIPI 1020

Query: 1021 MDDLSPKQLSRQGSLLNGFNRRHSDDEDPQAGRVLSSGDIPSSTGNLTSDKLFWTPFSEI 1080
            MDDLSPKQLSRQGSL NGFN RHSDDEDPQAGRVLSSGDIPSSTGNLT DKLFWTPFSEI
Sbjct: 1021 MDDLSPKQLSRQGSLSNGFNHRHSDDEDPQAGRVLSSGDIPSSTGNLTLDKLFWTPFSEI 1080

Query: 1081 QQMRMRDIQRSYFPELKSISNYTPKLLPTAHEFINKEGQKLHIFLSNEKFIVSDYEGELS 1140
            QQMRMRDIQRSYFPELKSISNYTPKLLPTAH+FINKEGQKLHIFLSNEKFIVSDYEGELS
Sbjct: 1081 QQMRMRDIQRSYFPELKSISNYTPKLLPTAHDFINKEGQKLHIFLSNEKFIVSDYEGELS 1140

Query: 1141 SIVACVLALLKDLSLETDLHNEDSKGEGLNKVPSSGSSDSDSSVTSEEHQFSSFDRLNLL 1200
            SIVACVLALLKDLSLETDLHNEDSKGEGLNKVPS+GSSDSDSSV+SEEHQFSSFDRLNLL
Sbjct: 1141 SIVACVLALLKDLSLETDLHNEDSKGEGLNKVPSNGSSDSDSSVSSEEHQFSSFDRLNLL 1200

Query: 1201 DSLLPKTFKRAEHEGVIKSLAKGKYLVNCPYFNQFRDLRRRCCPSELHYIASLSRCINWN 1260
            DSLLPKTFKRAEHEGVIKSLAKGKYLVNCPYFNQFRDLRRRCCPSELHYIASLSRCI+WN
Sbjct: 1201 DSLLPKTFKRAEHEGVIKSLAKGKYLVNCPYFNQFRDLRRRCCPSELHYIASLSRCIHWN 1260

Query: 1261 AKGGKSKSFFAKTLDDRFIIKEIKRTEYESFMKFAPDYFKYINESFDMGNQTCLAKVLGI 1320
            AKGGKSKSFFAKTLDDRFIIKEIKRTEYESFMKFAPDYFKYINESFDMGNQTCLAKVLGI
Sbjct: 1261 AKGGKSKSFFAKTLDDRFIIKEIKRTEYESFMKFAPDYFKYINESFDMGNQTCLAKVLGI 1320

Query: 1321 YQVTVREPKSGKEVRHELMVMENLSFGRNIIRQYDLKGALHARYNPATNDFGEVLLDQNF 1380
            YQVTVR+PKSGKEVRHELMVMENLSFGRNIIRQYDLKGALHARYNPATNDFGEVLLDQNF
Sbjct: 1321 YQVTVRDPKSGKEVRHELMVMENLSFGRNIIRQYDLKGALHARYNPATNDFGEVLLDQNF 1380

Query: 1381 VNDMNSSPLYVNNQAKRRLQRAIWNDTAFLNSMNVMDYSLLVGVDAKKKELVCGIIDYLR 1440
            VNDMNSSPLYVNNQAKRRLQRAIWNDTAFLNSMNVMDYSLLVGVDA+KKELVCGIIDYLR
Sbjct: 1381 VNDMNSSPLYVNNQAKRRLQRAIWNDTAFLNSMNVMDYSLLVGVDAEKKELVCGIIDYLR 1440

Query: 1441 QYTWDKQLETWAKSSLRPKNVLPTVVSPKEYKRRFRKFMSAHFLSVPDNWCQQQNLHGPW 1500
            QYTWDKQLETWAKSSLRPKNVLPTVVSPKEYKRRFRKFMSAHFLSVPDNWCQQQNLHGPW
Sbjct: 1441 QYTWDKQLETWAKSSLRPKNVLPTVVSPKEYKRRFRKFMSAHFLSVPDNWCQQQNLHGPW 1500

Query: 1501 GACGGTIDHASSQTNAEENDLSS 1523
            GACGGTID ASSQTNAEENDLSS
Sbjct: 1501 GACGGTIDRASSQTNAEENDLSS 1523

BLAST of CmaCh09G011720 vs. NCBI nr
Match: KAG6592249.1 (putative 1-phosphatidylinositol-3-phosphate 5-kinase FAB1D, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 2975.7 bits (7713), Expect = 0.0e+00
Identity = 1491/1523 (97.90%), Postives = 1507/1523 (98.95%), Query Frame = 0

Query: 1    MCHYCGAVITDSEGKRLGNGNSFDLDDSGTVCICRVCKENQELEAMNLDNKSSSEALMLS 60
            MCHYCGAVITDSEGKRLGNGNSFDLDDSGTVCIC+VCKENQEL+ MNLDNKSSSEALMLS
Sbjct: 1    MCHYCGAVITDSEGKRLGNGNSFDLDDSGTVCICKVCKENQELKTMNLDNKSSSEALMLS 60

Query: 61   PMPSLSSCDSDCSTVANLDIKDGQEGTTRSSQDSIDCRQERMSQNWSEVVHSNGQLSGRD 120
            PMPSLSSCDSDCSTVANLD+KDGQ+GTTRSSQDSID RQERMSQNWSEVVHSNGQLSGRD
Sbjct: 61   PMPSLSSCDSDCSTVANLDVKDGQDGTTRSSQDSIDGRQERMSQNWSEVVHSNGQLSGRD 120

Query: 121  DESVITISQEATHNGSGVRVNAEIEQSHSNWLDTDLWDPPEPEDPQDHVEGGMGY-DDDD 180
            DESVITISQEATHNGSGVRVNAEIEQSHS+WLDTDLWDPPEPEDPQDHVEGGMGY DDDD
Sbjct: 121  DESVITISQEATHNGSGVRVNAEIEQSHSDWLDTDLWDPPEPEDPQDHVEGGMGYDDDDD 180

Query: 181  DDEFDDSTEWNTSSSFSRSVDEASISYRIKEQKQRAMQQIMNGKFKVFIGHLLKFVGVAS 240
            DDEFDDSTEWNTSSSFSRSVDEAS+SYRIKEQKQRAMQQIMNGKFKVFIGHLLKFVGVAS
Sbjct: 181  DDEFDDSTEWNTSSSFSRSVDEASVSYRIKEQKQRAMQQIMNGKFKVFIGHLLKFVGVAS 240

Query: 241  SGEGSENWVDIVSSLSWEAATFLKPVVNGKAVDPEAHVKVKCIATGTRSQSQFVKGMVFK 300
            SGE SENWVDIVSSLSWEAATFLKPVVNGKAVDPEAHVKVKCIATGTRSQSQFVKGMVFK
Sbjct: 241  SGEDSENWVDIVSSLSWEAATFLKPVVNGKAVDPEAHVKVKCIATGTRSQSQFVKGMVFK 300

Query: 301  KHAAHKHMPTHCKNPKLILIEGMLGEAPISRLSSFNSMDQENDFTKHVIEMIEVCTANVI 360
            KHAAHKHMPTHCKNPKLILIEGMLGEAPISRLSSFNSMDQENDFTKHVIEMIEVCTANVI
Sbjct: 301  KHAAHKHMPTHCKNPKLILIEGMLGEAPISRLSSFNSMDQENDFTKHVIEMIEVCTANVI 360

Query: 361  LVEKTTSRVIQEAMLKKGMTLVLDMKLHRLERIALCTGSPILTSETLMSQNTRQCDAVYF 420
            LVEKTTSRVIQEAMLKKGMTLVLDMKLHRLERIALCTGSPILTSETLMSQNTRQCDAVYF
Sbjct: 361  LVEKTTSRVIQEAMLKKGMTLVLDMKLHRLERIALCTGSPILTSETLMSQNTRQCDAVYF 420

Query: 421  EKIVEEHAGLLEGGKRPTKTLMFIEGCPTRLGCTILLKGAHSDELKKIKVVVQIAVVMAF 480
            EKIVEEHAGLLEGGKRPTKTLMFIEGCPTRLGCTILLKGAHSDELKKIKVVVQIAVVMAF
Sbjct: 421  EKIVEEHAGLLEGGKRPTKTLMFIEGCPTRLGCTILLKGAHSDELKKIKVVVQIAVVMAF 480

Query: 481  HLMLETSFLVDQRAMFATIPFGGVPATVSSDPQSPLEPCSPNAQGASNGSNLEGEPYNAA 540
            HLMLETSFLVDQRAMFATIPFGGVPATVSSDPQSPLEPCSPNAQGASNGSNLEGEPYN A
Sbjct: 481  HLMLETSFLVDQRAMFATIPFGGVPATVSSDPQSPLEPCSPNAQGASNGSNLEGEPYNPA 540

Query: 541  IFSGLSSISDSLKRVMGESFFLASPCQSFSSYFGHGKDLSGLVAKSDSIPSTPPEADQFD 600
            IFSGLSSISDSLKRVMGESFFLASPCQSFSSYFGHGKDLSGLVAKSDSIPSTPPEADQFD
Sbjct: 541  IFSGLSSISDSLKRVMGESFFLASPCQSFSSYFGHGKDLSGLVAKSDSIPSTPPEADQFD 600

Query: 601  VEVRGSSDEENSIHEQFVPHQSTFDEGFGFHETAPNYSEDKLPKKTPFNSQSILVLMSSR 660
            VEVRGSSDEENSIHEQFVPHQSTFDEGFGFHETAPNYSEDKLP+KTPFNSQSILVLMSSR
Sbjct: 601  VEVRGSSDEENSIHEQFVPHQSTFDEGFGFHETAPNYSEDKLPEKTPFNSQSILVLMSSR 660

Query: 661  NALKGTMCEQSHFSHIVFYKNFDAPLGKFLQENLLNQKNVCTVCGELPEAHYYYYAHSRK 720
            NA KGTMCEQSHFSHIVFYKNFDAPLGKFLQENLLNQKNVCTVCGELPEAHYYYYAHSRK
Sbjct: 661  NASKGTMCEQSHFSHIVFYKNFDAPLGKFLQENLLNQKNVCTVCGELPEAHYYYYAHSRK 720

Query: 721  QLCIQVKQLPVDKVLPGETEGKLWMWSRCSKCKSKGGPSKSTKRVLISTAARGLSFGKFL 780
            QLCIQVKQLPV+KVLPGETEGKLWMWSRCSKCKSKGGPSKSTKRVLISTAARGLSFGKFL
Sbjct: 721  QLCIQVKQLPVNKVLPGETEGKLWMWSRCSKCKSKGGPSKSTKRVLISTAARGLSFGKFL 780

Query: 781  ELCFSDCTLPSKSSVCGHSLFGDFLYFFGLGNMVAMFRYTEVAIYTVSMPPQKLEFNNSM 840
            ELCFSDCTLPSKSSVCGHSLFGDFLYFFGLGNMVAMFRYTEVAIYTVSMPPQKLEFN+SM
Sbjct: 781  ELCFSDCTLPSKSSVCGHSLFGDFLYFFGLGNMVAMFRYTEVAIYTVSMPPQKLEFNSSM 840

Query: 841  RQGHLITETENVYEKGMILFTEIAQFLKKIISERKSSTVNDFSLLEEMLNEERSEFELNI 900
            RQGHLITETENVY KGMILFTEIAQFLKKIISERKSSTVNDFSLLEEMLNEERSEFELNI
Sbjct: 841  RQGHLITETENVYAKGMILFTEIAQFLKKIISERKSSTVNDFSLLEEMLNEERSEFELNI 900

Query: 901  QSSLTKKGNPDFPFHKYLSLNRLLWELLLESCIWDKRLQLLTSLGVTINSGTSENVEPEP 960
            QSSLTKKGNPDFPFHKYLSLNRLLWELLLESCIWDKRLQLLTS+GVT+NSGTSENVEPEP
Sbjct: 901  QSSLTKKGNPDFPFHKYLSLNRLLWELLLESCIWDKRLQLLTSVGVTLNSGTSENVEPEP 960

Query: 961  VRHEGLESIAQTDDRNVQQDVSVDENALHSKEIAVEHLDGESDGDELGLPSATEVTEIPI 1020
            V H GLESIAQTDDRNVQQDVSVDENALH KEIAVEHLDGESDGDELGLP ATEVTEIPI
Sbjct: 961  VGHGGLESIAQTDDRNVQQDVSVDENALHPKEIAVEHLDGESDGDELGLPLATEVTEIPI 1020

Query: 1021 MDDLSPKQLSRQGSLLNGFNRRHSDDEDPQAGRVLSSGDIPSSTGNLTSDKLFWTPFSEI 1080
            MDDLSPKQLSRQGSL NGFN RHSDDEDPQAGRVLSSGDIPSSTGNLT DKLFWTPFSEI
Sbjct: 1021 MDDLSPKQLSRQGSLSNGFNHRHSDDEDPQAGRVLSSGDIPSSTGNLTLDKLFWTPFSEI 1080

Query: 1081 QQMRMRDIQRSYFPELKSISNYTPKLLPTAHEFINKEGQKLHIFLSNEKFIVSDYEGELS 1140
            QQMRMRDIQRSYFPELKSISNYTPKLLPTAH+FINKEGQKLHIFLSNEKFIVSDYEGELS
Sbjct: 1081 QQMRMRDIQRSYFPELKSISNYTPKLLPTAHDFINKEGQKLHIFLSNEKFIVSDYEGELS 1140

Query: 1141 SIVACVLALLKDLSLETDLHNEDSKGEGLNKVPSSGSSDSDSSVTSEEHQFSSFDRLNLL 1200
            SIVACVLALLKDLSLETDLHNEDSKGEGLNKVPS+GSSDSDSSV+SEEHQFSSFDRLNLL
Sbjct: 1141 SIVACVLALLKDLSLETDLHNEDSKGEGLNKVPSNGSSDSDSSVSSEEHQFSSFDRLNLL 1200

Query: 1201 DSLLPKTFKRAEHEGVIKSLAKGKYLVNCPYFNQFRDLRRRCCPSELHYIASLSRCINWN 1260
            DSLLPKTFKRAEHEGVIKSLAKGKYLVNCPYFNQFRDLRRRCCPSELHYIASLSRCI+WN
Sbjct: 1201 DSLLPKTFKRAEHEGVIKSLAKGKYLVNCPYFNQFRDLRRRCCPSELHYIASLSRCIHWN 1260

Query: 1261 AKGGKSKSFFAKTLDDRFIIKEIKRTEYESFMKFAPDYFKYINESFDMGNQTCLAKVLGI 1320
            AKGGKSKSFFAKTLDDRFIIKEIKRTEYESFMKFAPDYFKYINESFDMGNQTCLAKVLGI
Sbjct: 1261 AKGGKSKSFFAKTLDDRFIIKEIKRTEYESFMKFAPDYFKYINESFDMGNQTCLAKVLGI 1320

Query: 1321 YQVTVREPKSGKEVRHELMVMENLSFGRNIIRQYDLKGALHARYNPATNDFGEVLLDQNF 1380
            YQVTVREPKSGKEVRHELMVMENLSFGRNIIRQYDLKGALHARYNPATNDFGEVLLDQNF
Sbjct: 1321 YQVTVREPKSGKEVRHELMVMENLSFGRNIIRQYDLKGALHARYNPATNDFGEVLLDQNF 1380

Query: 1381 VNDMNSSPLYVNNQAKRRLQRAIWNDTAFLNSMNVMDYSLLVGVDAKKKELVCGIIDYLR 1440
            VNDMNSSPLYVNNQAKRRLQRAIWNDTAFLNSMNVMDYSLLVGVDA+KKELVCGIIDYLR
Sbjct: 1381 VNDMNSSPLYVNNQAKRRLQRAIWNDTAFLNSMNVMDYSLLVGVDAEKKELVCGIIDYLR 1440

Query: 1441 QYTWDKQLETWAKSSLRPKNVLPTVVSPKEYKRRFRKFMSAHFLSVPDNWCQQQNLHGPW 1500
            QYTWDKQLETWAKSSLRPKNVLPTVVSPKEYKRRFRKFMSAHFLSVPDNWCQQQNLHGPW
Sbjct: 1441 QYTWDKQLETWAKSSLRPKNVLPTVVSPKEYKRRFRKFMSAHFLSVPDNWCQQQNLHGPW 1500

Query: 1501 GACGGTIDHASSQTNAEENDLSS 1523
            GAC GTID ASSQTNAEENDLSS
Sbjct: 1501 GACDGTIDRASSQTNAEENDLSS 1523

BLAST of CmaCh09G011720 vs. NCBI nr
Match: XP_023535373.1 (putative 1-phosphatidylinositol-3-phosphate 5-kinase FAB1D [Cucurbita pepo subsp. pepo] >XP_023535374.1 putative 1-phosphatidylinositol-3-phosphate 5-kinase FAB1D [Cucurbita pepo subsp. pepo])

HSP 1 Score: 2973.7 bits (7708), Expect = 0.0e+00
Identity = 1492/1523 (97.96%), Postives = 1507/1523 (98.95%), Query Frame = 0

Query: 1    MCHYCGAVITDSEGKRLGNGNSFDLDDSGTVCICRVCKENQELEAMNLDNKSSSEALMLS 60
            MCHYCGAVITDSEGKRLGNGNSFDLDDSGTVCIC+VCKENQELE MNLDNKSSSE LMLS
Sbjct: 1    MCHYCGAVITDSEGKRLGNGNSFDLDDSGTVCICKVCKENQELETMNLDNKSSSEVLMLS 60

Query: 61   PMPSLSSCDSDCSTVANLDIKDGQEGTTRSSQDSIDCRQERMSQNWSEVVHSNGQLSGRD 120
            PMPSLSSCDSDCSTVANLDIKDGQEGTTRSSQDSIDCRQERMSQNWSEVVHSNGQLSGRD
Sbjct: 61   PMPSLSSCDSDCSTVANLDIKDGQEGTTRSSQDSIDCRQERMSQNWSEVVHSNGQLSGRD 120

Query: 121  DESVITISQEATHNGSGVRVNAEIEQSHSNWLDTDLWDPPEPEDPQDHVEGGMGY-DDDD 180
            DESVITISQEATHNGSGVRVNAEIEQSHSNWLDTDLWDPPEPEDPQDHVEGGMGY DDDD
Sbjct: 121  DESVITISQEATHNGSGVRVNAEIEQSHSNWLDTDLWDPPEPEDPQDHVEGGMGYDDDDD 180

Query: 181  DDEFDDSTEWNTSSSFSRSVDEASISYRIKEQKQRAMQQIMNGKFKVFIGHLLKFVGVAS 240
            DDEFDDSTEWNTSSSFSRSVDEAS+SYRIKEQKQRAMQQIMNGKFKVFIGHLLKFVGVAS
Sbjct: 181  DDEFDDSTEWNTSSSFSRSVDEASVSYRIKEQKQRAMQQIMNGKFKVFIGHLLKFVGVAS 240

Query: 241  SGEGSENWVDIVSSLSWEAATFLKPVVNGKAVDPEAHVKVKCIATGTRSQSQFVKGMVFK 300
            SGE SENWVDIVSSLSWEAATFLKPVVNGKAVDPEAHVKVKCIATGTRSQSQFVKGMVFK
Sbjct: 241  SGEDSENWVDIVSSLSWEAATFLKPVVNGKAVDPEAHVKVKCIATGTRSQSQFVKGMVFK 300

Query: 301  KHAAHKHMPTHCKNPKLILIEGMLGEAPISRLSSFNSMDQENDFTKHVIEMIEVCTANVI 360
            KHAAHKHMPTHCKNPKLILIEGMLGEAPISRLSSFNSMDQENDFTKHVIEMIEVCTANVI
Sbjct: 301  KHAAHKHMPTHCKNPKLILIEGMLGEAPISRLSSFNSMDQENDFTKHVIEMIEVCTANVI 360

Query: 361  LVEKTTSRVIQEAMLKKGMTLVLDMKLHRLERIALCTGSPILTSETLMSQNTRQCDAVYF 420
            LVEKTTSRVIQEAMLKKGMTLVLDMKLHRLERIALCTGSPILTSETLMSQNTRQCDAVYF
Sbjct: 361  LVEKTTSRVIQEAMLKKGMTLVLDMKLHRLERIALCTGSPILTSETLMSQNTRQCDAVYF 420

Query: 421  EKIVEEHAGLLEGGKRPTKTLMFIEGCPTRLGCTILLKGAHSDELKKIKVVVQIAVVMAF 480
            EKIVEEHAGLLEGGKRPTKTLMFIEGCPTRLGCTILLKGAHSDELKKIKVVVQIAVVMAF
Sbjct: 421  EKIVEEHAGLLEGGKRPTKTLMFIEGCPTRLGCTILLKGAHSDELKKIKVVVQIAVVMAF 480

Query: 481  HLMLETSFLVDQRAMFATIPFGGVPATVSSDPQSPLEPCSPNAQGASNGSNLEGEPYNAA 540
            HLMLETSFLVDQRAMFATIPFGGVPATVSSDPQSPLEPCSPNAQGASNGSNLEGEPYN A
Sbjct: 481  HLMLETSFLVDQRAMFATIPFGGVPATVSSDPQSPLEPCSPNAQGASNGSNLEGEPYNPA 540

Query: 541  IFSGLSSISDSLKRVMGESFFLASPCQSFSSYFGHGKDLSGLVAKSDSIPSTPPEADQFD 600
            IFSGLSSISDSLKRVMGESFFLASPCQSFSSYFGHGKDLSGLVAKSDSIPSTPP ADQFD
Sbjct: 541  IFSGLSSISDSLKRVMGESFFLASPCQSFSSYFGHGKDLSGLVAKSDSIPSTPPGADQFD 600

Query: 601  VEVRGSSDEENSIHEQFVPHQSTFDEGFGFHETAPNYSEDKLPKKTPFNSQSILVLMSSR 660
            VEVRGSSDEENSIHEQFVPHQSTFDEGFGFHETAPNYSEDKL KKTPF+SQSILVLMSSR
Sbjct: 601  VEVRGSSDEENSIHEQFVPHQSTFDEGFGFHETAPNYSEDKLQKKTPFDSQSILVLMSSR 660

Query: 661  NALKGTMCEQSHFSHIVFYKNFDAPLGKFLQENLLNQKNVCTVCGELPEAHYYYYAHSRK 720
            NALKGTMCEQSHFSHIVFYKNFDAPLGKFLQENLLNQKNVCTVCGELPEAHYYYYAHSRK
Sbjct: 661  NALKGTMCEQSHFSHIVFYKNFDAPLGKFLQENLLNQKNVCTVCGELPEAHYYYYAHSRK 720

Query: 721  QLCIQVKQLPVDKVLPGETEGKLWMWSRCSKCKSKGGPSKSTKRVLISTAARGLSFGKFL 780
            QLCIQVKQLPV+KVLPGETEGKLWMWSRCSKCKSKGGPSKSTKRVLISTAARGLSFGKFL
Sbjct: 721  QLCIQVKQLPVNKVLPGETEGKLWMWSRCSKCKSKGGPSKSTKRVLISTAARGLSFGKFL 780

Query: 781  ELCFSDCTLPSKSSVCGHSLFGDFLYFFGLGNMVAMFRYTEVAIYTVSMPPQKLEFNNSM 840
            ELCFSDCTLPSKSSVCGHSLFGDFLYFFGLGNMVAMFRYTEVAIYTVSMPPQKLEFN+SM
Sbjct: 781  ELCFSDCTLPSKSSVCGHSLFGDFLYFFGLGNMVAMFRYTEVAIYTVSMPPQKLEFNSSM 840

Query: 841  RQGHLITETENVYEKGMILFTEIAQFLKKIISERKSSTVNDFSLLEEMLNEERSEFELNI 900
            RQGHLITETENVY KGMILFTEIAQFLKKIISERKSSTVNDFSLLEEMLNEERSEFE NI
Sbjct: 841  RQGHLITETENVYAKGMILFTEIAQFLKKIISERKSSTVNDFSLLEEMLNEERSEFEQNI 900

Query: 901  QSSLTKKGNPDFPFHKYLSLNRLLWELLLESCIWDKRLQLLTSLGVTINSGTSENVEPEP 960
            QSSLTKKGNPDFPFHKYLSLNRLLWELLLESCIWDKRLQLLTS+GVT+NSGTSENVEPEP
Sbjct: 901  QSSLTKKGNPDFPFHKYLSLNRLLWELLLESCIWDKRLQLLTSVGVTLNSGTSENVEPEP 960

Query: 961  VRHEGLESIAQTDDRNVQQDVSVDENALHSKEIAVEHLDGESDGDELGLPSATEVTEIPI 1020
            V HEGLESIAQTDDRNVQQDVSVDENALH KEIAVEHLDGESDGDELGLPSATEVTEIPI
Sbjct: 961  VGHEGLESIAQTDDRNVQQDVSVDENALHPKEIAVEHLDGESDGDELGLPSATEVTEIPI 1020

Query: 1021 MDDLSPKQLSRQGSLLNGFNRRHSDDEDPQAGRVLSSGDIPSSTGNLTSDKLFWTPFSEI 1080
            MDDLSPKQLSRQGSL NGFNRRHSDDEDPQAGRVLSSGDIPSSTGNLT DKLFWTPFSEI
Sbjct: 1021 MDDLSPKQLSRQGSLSNGFNRRHSDDEDPQAGRVLSSGDIPSSTGNLTLDKLFWTPFSEI 1080

Query: 1081 QQMRMRDIQRSYFPELKSISNYTPKLLPTAHEFINKEGQKLHIFLSNEKFIVSDYEGELS 1140
            QQMRMRDIQRSYFPELKSISNYTPKLLPTAH+FINKEGQKLHIFLSNEKFIVSDYEGELS
Sbjct: 1081 QQMRMRDIQRSYFPELKSISNYTPKLLPTAHDFINKEGQKLHIFLSNEKFIVSDYEGELS 1140

Query: 1141 SIVACVLALLKDLSLETDLHNEDSKGEGLNKVPSSGSSDSDSSVTSEEHQFSSFDRLNLL 1200
            SIVACVLALLKDLSLETDLHNEDSKGEGLNKVPS+GSSDSDSSV+SEEHQFSSFDRLNLL
Sbjct: 1141 SIVACVLALLKDLSLETDLHNEDSKGEGLNKVPSNGSSDSDSSVSSEEHQFSSFDRLNLL 1200

Query: 1201 DSLLPKTFKRAEHEGVIKSLAKGKYLVNCPYFNQFRDLRRRCCPSELHYIASLSRCINWN 1260
            DSLLPKTFKRAEHEG+IKSLAKGKYLVNCPYFNQFRDLRRRCCPSELHYIASLSRCI+WN
Sbjct: 1201 DSLLPKTFKRAEHEGIIKSLAKGKYLVNCPYFNQFRDLRRRCCPSELHYIASLSRCIHWN 1260

Query: 1261 AKGGKSKSFFAKTLDDRFIIKEIKRTEYESFMKFAPDYFKYINESFDMGNQTCLAKVLGI 1320
            AKGGKSKSFFAKTLDDRFIIKEIKRTEYESFMKFAPDYFKYINESFDMGNQ+CLAKVLGI
Sbjct: 1261 AKGGKSKSFFAKTLDDRFIIKEIKRTEYESFMKFAPDYFKYINESFDMGNQSCLAKVLGI 1320

Query: 1321 YQVTVREPKSGKEVRHELMVMENLSFGRNIIRQYDLKGALHARYNPATNDFGEVLLDQNF 1380
            YQVTVR+PKSGKEVRHELMVMENLSFGRNIIRQYDLKGALHARYNPATNDFGEVLLDQNF
Sbjct: 1321 YQVTVRDPKSGKEVRHELMVMENLSFGRNIIRQYDLKGALHARYNPATNDFGEVLLDQNF 1380

Query: 1381 VNDMNSSPLYVNNQAKRRLQRAIWNDTAFLNSMNVMDYSLLVGVDAKKKELVCGIIDYLR 1440
            VNDMNSSPLYVNNQAKRRLQRAIWNDTAFLNSMNVMDYSLLVGVDA+KKELVCGIIDYLR
Sbjct: 1381 VNDMNSSPLYVNNQAKRRLQRAIWNDTAFLNSMNVMDYSLLVGVDAEKKELVCGIIDYLR 1440

Query: 1441 QYTWDKQLETWAKSSLRPKNVLPTVVSPKEYKRRFRKFMSAHFLSVPDNWCQQQNLHGPW 1500
            QYTWDKQLETWAKSSLRPKNVLPTVVSPKEYKRRFRKFMSAHFLSVPDNWCQQQNLHGPW
Sbjct: 1441 QYTWDKQLETWAKSSLRPKNVLPTVVSPKEYKRRFRKFMSAHFLSVPDNWCQQQNLHGPW 1500

Query: 1501 GACGGTIDHASSQTNAEENDLSS 1523
            GACG T D ASSQTNAEENDLSS
Sbjct: 1501 GACGIT-DRASSQTNAEENDLSS 1522

BLAST of CmaCh09G011720 vs. TAIR 10
Match: AT1G34260.1 (FORMS APLOID AND BINUCLEATE CELLS 1A )

HSP 1 Score: 1117.8 bits (2890), Expect = 0.0e+00
Identity = 673/1513 (44.48%), Postives = 910/1513 (60.15%), Query Frame = 0

Query: 59   LSPMPSLSSCDSDCSTVANLDIKDGQEG-----TTRSSQDSIDCRQERMSQNWSEVVHSN 118
            ++P  SLSS +   S   ++D      G     ++ SSQ+ ++  +E         V   
Sbjct: 1    MTPSNSLSSSERSLSGECSVDGNSCDRGIEDECSSHSSQEDVELTKE-------VKVDRL 60

Query: 119  GQLSGRDDESVITISQEATHNGSGVRVNAEIEQSHSNWLDTDLWDPPEPEDPQDHVEGGM 178
             + S      ++ I  E +   S   V   +     +  D  +W+PPEPE+P+D V+G  
Sbjct: 61   ERKSKSMPSDILDILDEKSKENSVENVQF-LSDREDDSDDVPVWEPPEPENPEDEVDGVF 120

Query: 179  GYDDDDDDEFDDSTEWNTSSSFSRSVDEASISYRIKEQKQRAMQQIMNGKFKVFIGHLLK 238
                DDDD+  D ++WN +S      DE+S   ++ E+ +R M +  + KFK  +  L+K
Sbjct: 121  A---DDDDDCCDGSKWNKASLLGELSDESSEKRKVYEENRRVMLEEADSKFKFIVSQLIK 180

Query: 239  FVGVASSGEGSENWVDIVSSLSWEAATFLKPVVNGKAVDPEAHVKVKCIATGTRSQSQFV 298
              G   S E S  W +IV+ L WEAA+ LKP ++GK+VDP  ++KVKCIATG+   S+  
Sbjct: 181  SAGF--SIEESGYWFEIVARLCWEAASMLKPAIDGKSVDPTEYIKVKCIATGSCVDSEVF 240

Query: 299  KGMVFKKHAAHKHMPTHCKNPKLILIEGMLGEAPISRLSSFNSMDQEND----FTKHVIE 358
            KG+VFKKHAA KHM T  ++P+++L+EG+LG  PIS  SS  S++Q+N+    + K V++
Sbjct: 241  KGLVFKKHAALKHMATKYEHPRIMLVEGVLGH-PISGFSSLQSVNQDNEYLLKYVKPVVD 300

Query: 359  MIEVCTANVILVEKTTSRVIQEAMLKKGMTLVLDMKLHRLERIALCTGSPILTSETLMSQ 418
            +IE    +V+LVEK+ SR IQ+ +L KG+TLV DMKLHRL+RI+ C GSPIL+ ++L SQ
Sbjct: 301  IIEASKPDVMLVEKSVSRDIQKTILDKGVTLVFDMKLHRLQRISRCIGSPILSVDSLSSQ 360

Query: 419  NTRQCDAVYFEKIVEEHAGLLEGGKRPTKTLMFIEGCPTRLGCTILLKGAHSDELKKIKV 478
              + CD+   EKIVEEH    E  K+PTKTLMF+EGCPTRLGCTILLKG HS+ LKK+K 
Sbjct: 361  KLKHCDSFRIEKIVEEHNAAGESDKKPTKTLMFLEGCPTRLGCTILLKGCHSERLKKVKE 420

Query: 479  VVQIAVVMAFHLMLETSFLVDQRAMFATI------------------------PFGGVPA 538
            VVQ + ++A+HLMLE SFL D+  MF+TI                        P   V  
Sbjct: 421  VVQYSFILAYHLMLEASFLADRHTMFSTIFAKEATSCVVEIENFSPSPSPRESPSEAVDI 480

Query: 539  TVSSDPQSPLEPCSPNAQG------ASNGSNL-EGEPYNAAIFSGLSSISDSLKRVMGES 598
             VS+         +  A G       S+G ++   EPYN  IF+G SS+S  L       
Sbjct: 481  PVSNGFDEQTIQINGEADGEKVGTWESDGDHVFSHEPYNPVIFTGFSSLSARL------- 540

Query: 599  FFLASPCQSFSSYFGHGKDLSGLVAKSDSIPSTPPEADQF----DVEVRGSSDEENSIHE 658
                      S Y G  ++   +    D+  ST    D      +     + D++  + +
Sbjct: 541  ----------SKYLGFVQNPESVPVSVDTDVSTTSNLDSIRESEEDTAEKNEDKQPLLLD 600

Query: 659  QFVPHQSTFDEGFGFHETAPNYSEDKLPKKTPFNSQSILVLMSSRNALKGTMCEQSHFSH 718
              +P  S+ D+G        N S+ +   ++   SQSILVL+S RNAL+G MC+Q HFSH
Sbjct: 601  PELPVNSSSDDG-------DNKSQTENDIESTLESQSILVLVSKRNALRGIMCDQRHFSH 660

Query: 719  IVFYKNFDAPLGKFLQENLLNQKNVCTVCGELPEAHYYYYAHSRKQLCIQVKQLPVDKVL 778
            I FYK+FD PL KFL++ + NQ+N+C  C E PEAH YYYAH  KQL IQ+K++PV K L
Sbjct: 661  IKFYKHFDVPLEKFLRD-MFNQRNLCQTCVEFPEAHLYYYAHQNKQLTIQIKRIPVAKGL 720

Query: 779  PGETEGKLWMWSRCSKCKSKGGPSKSTKRVLISTAARGLSFGKFLELCFSDCTLPSKSSV 838
             GE +GK+WMWSRC KCK+K    KSTKRVLISTAAR LSFGKFLEL FS  T  ++SS 
Sbjct: 721  AGEAKGKIWMWSRCGKCKTKNASRKSTKRVLISTAARSLSFGKFLELSFSQQTFLNRSSS 780

Query: 839  CGHSLFGDFLYFFGLGNMVAMFRYTEVAIYTVSMPPQKLEFNNSMRQGHLITETENVYEK 898
            CGHS   DFL+FFGLG+MVAM  Y++VA YTVS+PP KLE +  ++ G L  E + V+ K
Sbjct: 781  CGHSFDSDFLHFFGLGSMVAMLSYSQVASYTVSLPPMKLESSILIKAGWLEKEFQTVFTK 840

Query: 899  GMILFTEIAQFLKKIISERKSSTVND------FSLLEEMLNEERSEFELNIQSSLTKKGN 958
            G+ LF + A FLK++ S+  +S +         S +EE+L  ER  FE NI++S  K   
Sbjct: 841  GISLFEDAAGFLKRLRSQFTNSDLRYQRARKLLSNIEELLKHERCIFEENIKNSFDKAKT 900

Query: 959  PDFPFHKYLSLNRLLWELLLESCIWDKRLQLLTSLGVTINSGTSENVEPEPVRHEGLESI 1018
             D   H+ L LNR+ WELLL++ IW+ RLQ L      ++     + +   +  +GL+++
Sbjct: 901  IDDVSHRLLRLNRMRWELLLQALIWNYRLQSL-----VLSDRLLPSSDETKIYEQGLKTV 960

Query: 1019 AQTDDRNVQQDVSVDENALHS--KEIAVEHLD------GESDGDELGLPSATEVTEIPIM 1078
            ++      + D  V ++  +       VEH D         D D++      E  E   +
Sbjct: 961  SEAGMTRYENDNKVSDSGSNGGIDTPLVEHKDIPIAGASVGDNDQMAESYVPEDNESQTL 1020

Query: 1079 DDLSPKQLS--------RQGSLLNGFNRRHSDDEDPQAGRVLSSGDIPSSTGN--LTSDK 1138
               SP   S             ++  N + +D   P  G  L   ++ +S G   L  D+
Sbjct: 1021 CSSSPDTTSPINNHFDTHLAVNVHSTNGQEADKSIPVTGESLDD-EVSTSNGPHILGWDE 1080

Query: 1139 LFWTPFSEIQQMRMRDIQRSYFPELKSISNYTPKLLPTAHEFINKEGQKLHIFLSNEKFI 1198
             FW PF E++  R+ DI++ Y  + + ++N+T + L T ++ I +E  +L I L ++ FI
Sbjct: 1081 WFWLPFEELRSKRIVDIEKEYLLKFEYVNNFTQENLQTVNQIITEESSRLRISLRDDDFI 1140

Query: 1199 VSDYEGELSSIVACVLALLKDLSLETDLHNEDSKGEGLNKVPSS----GSSDSDSSVTSE 1258
            VSDYE ELSS++AC LA          L+NE+SK      +  S      ++ DS  T  
Sbjct: 1141 VSDYEDELSSLIACALA---------HLNNEESKKPLSRCIHGSLQGFLDNNQDSKQTDR 1200

Query: 1259 EHQFSSFDRLNLLDSLLPK----TFKRAEHEGVIKSLAKGKYLVNCPYFNQFRDLRRRCC 1318
            +    S +  N L++L P     TF      G +KS+ K KY +   Y + FRDLR+RCC
Sbjct: 1201 DVSRFSSESTNRLETLPPPEVLVTF------GSVKSVGKPKYSIVSLYADDFRDLRKRCC 1260

Query: 1319 PSELHYIASLSRCINWNAKGGKSKSFFAKTLDDRFIIKEIKRTEYESFMKFAPDYFKYIN 1378
             SEL YIASLSRC  W+AKGGKSKS FAKTLDDRFI+KEIK+TEYESF+ FA +YFKY+ 
Sbjct: 1261 SSELDYIASLSRCKPWDAKGGKSKSVFAKTLDDRFIVKEIKKTEYESFVTFATEYFKYMK 1320

Query: 1379 ESFDMGNQTCLAKVLGIYQVTVREPK-SGKEVRHELMVMENLSFGRNIIRQYDLKGALHA 1438
            +S+D+GNQTCLAKVLGI+QVTVR+PK  GKE+RH+LMVMENLSF R + RQYDLKGALHA
Sbjct: 1321 DSYDLGNQTCLAKVLGIHQVTVRQPKGGGKEIRHDLMVMENLSFSRKVTRQYDLKGALHA 1380

Query: 1439 RYN-PATNDFGEVLLDQNFVNDMNSSPLYVNNQAKRRLQRAIWNDTAFLNSMNVMDYSLL 1493
            R+   + N   +VLLDQNFVNDMN SPLYV+  +K+ LQRA++NDT+FL S+NVMDYSLL
Sbjct: 1381 RFTATSANGEDDVLLDQNFVNDMNKSPLYVSKTSKQNLQRAVYNDTSFLTSINVMDYSLL 1440

BLAST of CmaCh09G011720 vs. TAIR 10
Match: AT3G14270.1 (phosphatidylinositol-4-phosphate 5-kinase family protein )

HSP 1 Score: 770.4 bits (1988), Expect = 2.7e-222
Identity = 545/1476 (36.92%), Postives = 778/1476 (52.71%), Query Frame = 0

Query: 156  LWDPPEPEDPQDHVEGGMGYDDDDDDEFDDSTEW---NTSSSFSRSVDEASISYRIKEQK 215
            LW PPEPE+ +D  E  +   D++D+E D S EW     S+SF     E     R  E+ 
Sbjct: 318  LWVPPEPENEEDERESAL--FDEEDNEGDASGEWGYLRPSTSFGSG--EYRGEDRTTEEH 377

Query: 216  QRAMQQIMNGKFKVFIGHLLKFVGVA-SSGEGSENWVDIVSSLSWEAATFLKP-VVNGKA 275
            ++AM+ +++G F+  +  LL+   ++ S  EG E+W++I++SLSWEAA  LKP +     
Sbjct: 378  KKAMKNVVDGHFRALLAQLLQVENISVSDEEGKESWLEIITSLSWEAANLLKPDMSKSGG 437

Query: 276  VDPEAHVKVKCIATGTRSQSQFVKGMVFKKHAAHKHMPTHCKNPKLILIEGMLGEAPIS- 335
            +DP  +VKVKC+A+G R  S  VKG+V KK+  ++ M T  +  +L+++ G L    +S 
Sbjct: 438  MDPGGYVKVKCLASGFRHDSMVVKGVVCKKNVVNRRMSTKIEKARLLILGGGLEYQRVSN 497

Query: 336  RLSSFNS-MDQENDFTKHVIEMIEVCTANVILVEKTTSRVIQEAMLKKGMTLVLDMKLHR 395
            +LSSF++ + QE D  K  +  I     N++LVEK+ SR  QE +L K ++LVL++K   
Sbjct: 498  QLSSFDTLLQQEKDHLKMAVAKIHAERPNILLVEKSVSRFAQEYLLAKDISLVLNIKRPL 557

Query: 396  LERIALCTGSPILTS-ETLMSQNTRQCDAVYFEKIVEEHAGLLEGGKRPTKTLMFIEGCP 455
            L+RIA CTG+ I+ S + L SQ    C+    ++  EEH    + GK+  KTLM+ E CP
Sbjct: 558  LDRIARCTGAQIIPSVDHLSSQKLGYCENFRVDRYPEEHGSTGQVGKKVVKTLMYFEHCP 617

Query: 456  TRLGCTILLKGAHSDELKKIKVVVQIAVVMAFHLMLETSFLVDQRA---------MFATI 515
              LG TILL+GA+ DELKK+K VVQ  V  A+HL LETSFL D+ A         +   +
Sbjct: 618  KPLGFTILLRGANEDELKKVKHVVQYGVFAAYHLALETSFLADEGASPELPLNSPITVAL 677

Query: 516  P---------FGGVPA-TVSSDPQSP--LEPCSPNAQGASNGSNLEGEPYNAAIFSGLSS 575
            P            VP  TVS+  +SP  L    P    +   S L     N +I   +  
Sbjct: 678  PDKSTSIERSISTVPGFTVSTYEKSPTMLSCAEPQRANSVPVSELLSTTTNLSIQKDIPP 737

Query: 576  I---SDSLKRVMGESFFLASPCQSFS----SYFGHGKDLSGLVAKSDSIPSTPPEADQFD 635
            I   S    R +  SF  +    S +           DLSG      S+P   P AD+ +
Sbjct: 738  IPYGSGWQAREINPSFVFSRHNISLNLPDRVIESRNSDLSG-----RSVPVDTP-ADKSN 797

Query: 636  VEVRGSSDEENSIH---EQFVPHQSTF-------DEGFGFHETAPNYSEDKLPKKT---- 695
              V       NS+H   + FV   S         ++  G   T      ++ PK+T    
Sbjct: 798  PIVVADETTNNSLHLSGQGFVRKSSQIGTSIMVENQDNGSELTIAQQQNNEKPKETQSQK 857

Query: 696  ------PFNSQSILVLMSSRNALKGTMCEQSHFSHIVFYKNFDAPLGKFLQENLLNQKNV 755
                  P + QSILV +SSR+  KGT+CE+SH   I +Y +FD PLG+FL+++L +Q   
Sbjct: 858  EEFPPSPSDHQSILVSLSSRSVWKGTVCERSHLFRIKYYGSFDKPLGRFLRDHLFDQSYR 917

Query: 756  CTVCGELPEAHYYYYAHSRKQLCIQVKQLPVDKVLPGETEGKLWMWSRCSKCKSKGGPSK 815
            C  C    EAH + Y H +  L I VK+L  D +LPGE EGK+WMW RC +C    G   
Sbjct: 918  CRSCEMPSEAHVHCYTHRQGSLTISVKKLQ-DYLLPGEKEGKIWMWHRCLRCPRLNGFPP 977

Query: 816  STKRVLISTAARGLSFGKFLELCFSDCTLPSKSSVCGHSLFGDFLYFFGLGNMVAMFRYT 875
            +T RV++S AA GLSFGKFLEL FS+    S+ + CGHSL  D L F+G GNMVA FRY 
Sbjct: 978  ATLRVVMSDAAWGLSFGKFLELSFSNHAAASRVACCGHSLHRDCLRFYGFGNMVACFRYA 1037

Query: 876  EVAIYTVSMPPQKLEFNNSMRQGHLITETENVYEKGMILFTEIAQFLKKIISERKSSTVN 935
             + +++V +PP  L FN    Q  +  ET+ V E+  +LF+E+   + +I  +     + 
Sbjct: 1038 TIDVHSVYLPPSILSFNYE-NQDWIQRETDEVIERAELLFSEVLNAISQIAEKGFRRRIG 1097

Query: 936  DFSLLEEMLNEERSEFELNIQSSLTKKGNPDFPFHKYLSLNRLLWELLLESCIWDKRLQL 995
            +   LEE+L +E++EFE N+Q  L ++ N   P    L L R+  +LL +S +WD RL  
Sbjct: 1098 E---LEEVLQKEKAEFEENMQKILHREVNEGQPLVDILELYRIHRQLLFQSYMWDHRLIN 1157

Query: 996  LTSLGVTINSGTSENVEPE----------PVRHEGLESIAQTDDRNVQQ--DVSVDENAL 1055
             ++L    NS  ++  E E          P  + G  S+    + N+    D + D  +L
Sbjct: 1158 ASTLHKLENSDDTKREENEKPPLAKSQTLPEMNAGTNSLLTGSEVNLNPDGDSTGDTGSL 1217

Query: 1056 HSKEIAVEH----LDGESDGDELGLPSATEVTEIPIMDDLSPKQLSRQGSLL-------- 1115
            ++ +   +        + DG E+        T  P+ + +  ++    G ++        
Sbjct: 1218 NNVQKEADTNSDLYQEKDDGGEVSPSKTLPDTSYPLENKVDVRRTQSDGQIVMKNLSATL 1277

Query: 1116 --NGFNRRHSDDEDPQAGRV---------------LSSG--------------------- 1175
                   R +  E P   +V               +S G                     
Sbjct: 1278 DAAWIGERQTSVEIPTNNKVSLPPSTMSNSSTFPPISEGLMPIDLPEQQNEFKVAYPVSP 1337

Query: 1176 DIPSSTGNLTSDKLFW--TPFSEIQQMRMRDIQRSYF---PELKSISNYTPKLLPTAHEF 1235
             +PS     + D + W   PF        R I +++     +L +   ++P  + +  E 
Sbjct: 1338 ALPSKNYENSEDSVSWLSVPFLNF----YRSINKNFLLSSQKLDTFGEHSPIYISSFREA 1397

Query: 1236 INKEGQKLHIFLSNEKFIVSDYEGELSSIVACVLALLKDLSLETDLHNEDSKGEGLNKVP 1295
              + G +L + +     +V  Y+ E +S++A  L     +S E       ++GE L   P
Sbjct: 1398 ELQGGPRLLLPVGLNDIVVPVYDDEPTSMIAYAL-----MSPEYQ-RQTSAEGESLVSYP 1457

Query: 1296 SS------------GSSDSDSSVTSEEHQFSSFDRLNLLDSLLPKTFKRAEHE----GVI 1355
            S               S S+ SV       SS    +LLD   P ++ +A H     G  
Sbjct: 1458 SELNIPRPVDDTIFDPSRSNGSVDESILSISSSRSTSLLD---PLSYTKALHARVSYGED 1517

Query: 1356 KSLAKGKYLVNCPYFNQFRDLRRRCCPSELHYIASLSRCINWNAKGGKSKSFFAKTLDDR 1415
             +L K KY V C Y  +F  LR  C PSEL YI SLSRC  W A+GGKS  FFAKTLDDR
Sbjct: 1518 GTLGKVKYTVTCYYAKRFEALRGICLPSELEYIRSLSRCKKWGAQGGKSNVFFAKTLDDR 1577

Query: 1416 FIIKEIKRTEYESFMKFAPDYFKYINESFDMGNQTCLAKVLGIYQVTVREPKSGKEVRHE 1475
            FIIK++ +TE ESF+KFAP YFKY++ES    + TCLAK+LGIYQV  ++ KSGKE + +
Sbjct: 1578 FIIKQVTKTELESFIKFAPAYFKYLSESISTKSPTCLAKILGIYQVATKQLKSGKETKMD 1637

Query: 1476 LMVMENLSFGRNIIRQYDLKGALHARYNPATNDFGEVLLDQNFVNDMNSSPLYVNNQAKR 1490
            +++MENL FGR + R YDLKG+  ARYNP ++   +VLLDQN +  M +SP++V N+AKR
Sbjct: 1638 VLIMENLLFGRTVKRLYDLKGSSRARYNPDSSGSNKVLLDQNLIEAMPTSPIFVGNKAKR 1697

BLAST of CmaCh09G011720 vs. TAIR 10
Match: AT4G33240.1 (1-phosphatidylinositol-4-phosphate 5-kinases;zinc ion binding;1-phosphatidylinositol-3-phosphate 5-kinases )

HSP 1 Score: 740.7 bits (1911), Expect = 2.3e-213
Identity = 520/1435 (36.24%), Postives = 753/1435 (52.47%), Query Frame = 0

Query: 156  LWDPPEPEDPQDHVEGGMGYDDDDDDEFDDSTEWNTSSSFSRSVDEASISYRIKEQKQRA 215
            LW PPEPE+ +D  E  +  DD D+ +  D      S+SF+         +  K++   A
Sbjct: 311  LWLPPEPENEEDEREAVLSDDDGDEGDRGDWGYLRPSNSFNEK------DFHSKDKSSGA 370

Query: 216  MQQIMNGKFKVFIGHLLKFVGVASSGEG-SENWVDIVSSLSWEAATFLKP-VVNGKAVDP 275
            M+ ++ G F+  +  LL+   +    EG  E W+DI++SLSWEAAT LKP       +DP
Sbjct: 371  MKNVVEGHFRALVAQLLEVDNLPMVNEGDEEGWLDIITSLSWEAATLLKPDTSKSGGMDP 430

Query: 276  EAHVKVKCIATGTRSQSQFVKGMVFKKHAAHKHMPTHCKNPKLILIEGMLGEAPIS-RLS 335
              +VKVKCI  G RS+S  VKG+V KK+ AH+ M +  + P+L+++ G L    IS +LS
Sbjct: 431  GGYVKVKCIPCGRRSESMVVKGVVCKKNVAHRRMTSKIEKPRLLILGGALEYQRISNQLS 490

Query: 336  SFNS-MDQENDFTKHVIEMIEVCTANVILVEKTTSRVIQEAMLKKGMTLVLDMKLHRLER 395
            SF++ + QE D  K  +  I+    +++LVEK+ SR  QE +L K ++LVL++K   LER
Sbjct: 491  SFDTLLQQEMDHLKMAVAKIDSHNPDILLVEKSVSRFAQEYLLAKDISLVLNIKRSLLER 550

Query: 396  IALCTGSPILTS-ETLMSQNTRQCDAVYFEKIVEEHAGLLEGGKRPTKTLMFIEGCPTRL 455
            I+ CTG+ I+ S + L S     CD  + EK VE H    +  K+  KTLMF +GCP  L
Sbjct: 551  ISRCTGAQIVPSIDQLTSPKLGYCDLFHVEKFVETHVSPCQVAKKMAKTLMFFDGCPKPL 610

Query: 456  GCTILLKGAHSDELKKIKVVVQIAVVMAFHLMLETSFLVDQRAMFATIPFGGVPATVSSD 515
            GCTILLKGAH DELKK+K V+Q  V  A+HL LETSFL D+ A    +P    P TV+  
Sbjct: 611  GCTILLKGAHEDELKKVKHVIQYGVFAAYHLALETSFLADEGASIHELPL-QTPITVALP 670

Query: 516  PQ--------SPLEPCSPNAQGASNGSNLEGEPYNA---AIFSGLSSISDSLKRVMGESF 575
             +        S +   + ++   S  + L GEP+ A      +  SS +    ++ G   
Sbjct: 671  DKPSMVNRSISTIPGFTVSSAEKSPTTELRGEPHKANGDLTGNFTSSKTHFQGKLDGNDR 730

Query: 576  FLASPCQSFSSYFGHGKDLSGLVAKSDS-IPSTPPEADQFDVEVRGSSDEENSI----HE 635
               S     +    + K    + +K D  +P+       F VE      ++ S+     E
Sbjct: 731  IDPSERLLHNLDTVYCKPPETITSKDDGLVPTLESRQLSFHVEEPSVQKDQWSVLSGATE 790

Query: 636  QFVPHQSTFD------EGFGFHETAPNYSEDKLPKKTPFNSQSILVLMSSRNALKGTMCE 695
            Q      T D      + F   E   +   D  P  +  + QSILV +S+R   KG++CE
Sbjct: 791  QVTDGGYTNDSAVIGNQNFNRQEQMESSKGDFHPSAS--DHQSILVSLSTRCVWKGSVCE 850

Query: 696  QSHFSHIVFYKNFDAPLGKFLQENLLNQKNVCTVCGELPEAHYYYYAHSRKQLCIQVKQL 755
            ++H   I +Y +FD PLG+FL++NL +Q   C  C    EAH + Y H +  L I VK+L
Sbjct: 851  RAHLLRIKYYGSFDKPLGRFLRDNLFDQDQCCPSCTMPAEAHIHCYTHRQGSLTISVKKL 910

Query: 756  PVDKVLPGETEGKLWMWSRCSKCKSKGGPSKSTKRVLISTAARGLSFGKFLELCFSDCTL 815
            P  ++LPG+ EGK+WMW RC KC    G   +T+R+++S AA GLSFGKFLEL FS+   
Sbjct: 911  P--ELLPGQREGKIWMWHRCLKCPRINGFPPATRRIVMSDAAWGLSFGKFLELSFSNHAA 970

Query: 816  PSKSSVCGHSLFGDFLYFFGLGNMVAMFRYTEVAIYTVSMPPQKLEFNNSMRQGHLITET 875
             S+ + CGHSL  D L F+G G MVA FRY  + IY V++PP KL FN    Q  L  E+
Sbjct: 971  ASRVANCGHSLHRDCLRFYGFGRMVACFRYASINIYAVTLPPAKLYFNYE-NQEWLQKES 1030

Query: 876  ENVYEKGMILFTEIAQFLKKIISE-----RKSSTVNDFSL-LEE---MLNEERSEFELNI 935
            + V +K  +LF E+ + L +I ++      K ST N   L LEE   +L + + E++ ++
Sbjct: 1031 KEVIKKAEVLFNEVQEALSQISAKTMGAGSKGSTPNKIKLSLEELAGLLEQRKKEYKDSL 1090

Query: 936  QSSL--TKKGNPDFPFHKYLSLNRLLWELLLESCIWDKRLQLLTSLGVTINSGTSENVEP 995
            Q  L   K G P       L +N+L   ++ +S  WD+ L    ++         +N  P
Sbjct: 1091 QQMLNVVKDGQPTIDI---LLINKLRRLIIFDSYAWDECLAGAANMVRNNYLEAPKNSAP 1150

Query: 996  EPV-RHEGLESIAQTDDRNVQQDVSVDENAL------------------HSKEIAVEHLD 1055
            + + R+  LE ++    +++   V++  ++L                   S + A+    
Sbjct: 1151 KVMGRNVSLEKLSDEKVKSIPTHVAICNDSLLQDADYETCLNQGKSFADTSGKFAIPEDV 1210

Query: 1056 G---------ESDGDELGLPSATEVTEI-------------PIMDDLSPKQLSRQGSLLN 1115
            G         E D  E G  +  E +++              + D L    +  Q +  N
Sbjct: 1211 GSDRPPDCRMEFDPSEGGKDNFVESSQVVKPAHTESQFQATDLSDTLDAAWIGEQTTSEN 1270

Query: 1116 GFNR---RHSDDEDPQAG--RVLSSGDIPSSTGNLTSDKLFWTPFSEIQQMRMRDIQRSY 1175
            G  R   R +     Q    R+L S    +  G  T+D+   T            + ++Y
Sbjct: 1271 GIFRPPSRAASTNGTQIPDLRLLGSESELNFKGGPTNDE-HTTQVQLPSPSFYYSLNKNY 1330

Query: 1176 FPELKS--ISNYTPKLLPTAHEFINKEGQKLHIFLSNEKFIVSDYEGELSSIVACVLALL 1235
                +   ++   P  + +  E   + G +L + L     ++  Y+ E +SI+A  L   
Sbjct: 1331 SLNSRKHIMAEDRPVYVSSYRELEWRSGARLLLPLGCNDLVLPVYDDEPTSIIAYALT-- 1390

Query: 1236 KDLSLETDLHNEDSKGEGLNKVPSSGSSDSDSSVTSEEHQFSSFDRLNLLDSLLPKTFKR 1295
                 +  +   D   + L+   S    DS + ++       S D    L S   +  + 
Sbjct: 1391 -SSEYKAQMSGSDKSRDRLDSGGSFSLFDSVNLLSLNSLSDLSVDMSRSLSSADEQVSQL 1450

Query: 1296 AEHEGVIKSL------------AKGKYLVNCPYFNQFRDLRRRCCPSELHYIASLSRCIN 1355
                  +K L             K KY V C Y  +F  LR  CCPSE  +I SL RC  
Sbjct: 1451 LHSSLYLKDLHARISFTDEGPPGKVKYSVTCYYAKEFEALRMICCPSETDFIRSLGRCRK 1510

Query: 1356 WNAKGGKSKSFFAKTLDDRFIIKEIKRTEYESFMKFAPDYFKYINESFDMGNQTCLAKVL 1415
            W A+GGKS  FFAK+LDDRFIIK++ +TE ESF+KF P YFKY+ ES    + T LAK+L
Sbjct: 1511 WGAQGGKSNVFFAKSLDDRFIIKQVTKTELESFIKFGPAYFKYLTESISTKSPTSLAKIL 1570

Query: 1416 GIYQVTVREPKSGKEVRHELMVMENLSFGRNIIRQYDLKGALHARYNPATNDFGEVLLDQ 1475
            GIYQV+ +  K GKE + +++VMENL F RN  R YDLKG+  ARYNP T+    VLLDQ
Sbjct: 1571 GIYQVSSKHLKGGKEFKMDVLVMENLLFKRNFTRLYDLKGSTRARYNPDTSGSNTVLLDQ 1630

Query: 1476 NFVNDMNSSPLYVNNQAKRRLQRAIWNDTAFLNSMNVMDYSLLVGVDAKKKELVCGIIDY 1490
            N V  M +SP++V ++AKR L+RA+WNDT+FL S++VMDYSLLVGVD ++ ELV GIID+
Sbjct: 1631 NLVEAMPTSPIFVGSKAKRLLERAVWNDTSFLASIHVMDYSLLVGVDEERNELVLGIIDF 1690

BLAST of CmaCh09G011720 vs. TAIR 10
Match: AT4G33240.2 (1-phosphatidylinositol-4-phosphate 5-kinases;zinc ion binding;1-phosphatidylinositol-3-phosphate 5-kinases )

HSP 1 Score: 740.7 bits (1911), Expect = 2.3e-213
Identity = 520/1435 (36.24%), Postives = 753/1435 (52.47%), Query Frame = 0

Query: 156  LWDPPEPEDPQDHVEGGMGYDDDDDDEFDDSTEWNTSSSFSRSVDEASISYRIKEQKQRA 215
            LW PPEPE+ +D  E  +  DD D+ +  D      S+SF+         +  K++   A
Sbjct: 310  LWLPPEPENEEDEREAVLSDDDGDEGDRGDWGYLRPSNSFNEK------DFHSKDKSSGA 369

Query: 216  MQQIMNGKFKVFIGHLLKFVGVASSGEG-SENWVDIVSSLSWEAATFLKP-VVNGKAVDP 275
            M+ ++ G F+  +  LL+   +    EG  E W+DI++SLSWEAAT LKP       +DP
Sbjct: 370  MKNVVEGHFRALVAQLLEVDNLPMVNEGDEEGWLDIITSLSWEAATLLKPDTSKSGGMDP 429

Query: 276  EAHVKVKCIATGTRSQSQFVKGMVFKKHAAHKHMPTHCKNPKLILIEGMLGEAPIS-RLS 335
              +VKVKCI  G RS+S  VKG+V KK+ AH+ M +  + P+L+++ G L    IS +LS
Sbjct: 430  GGYVKVKCIPCGRRSESMVVKGVVCKKNVAHRRMTSKIEKPRLLILGGALEYQRISNQLS 489

Query: 336  SFNS-MDQENDFTKHVIEMIEVCTANVILVEKTTSRVIQEAMLKKGMTLVLDMKLHRLER 395
            SF++ + QE D  K  +  I+    +++LVEK+ SR  QE +L K ++LVL++K   LER
Sbjct: 490  SFDTLLQQEMDHLKMAVAKIDSHNPDILLVEKSVSRFAQEYLLAKDISLVLNIKRSLLER 549

Query: 396  IALCTGSPILTS-ETLMSQNTRQCDAVYFEKIVEEHAGLLEGGKRPTKTLMFIEGCPTRL 455
            I+ CTG+ I+ S + L S     CD  + EK VE H    +  K+  KTLMF +GCP  L
Sbjct: 550  ISRCTGAQIVPSIDQLTSPKLGYCDLFHVEKFVETHVSPCQVAKKMAKTLMFFDGCPKPL 609

Query: 456  GCTILLKGAHSDELKKIKVVVQIAVVMAFHLMLETSFLVDQRAMFATIPFGGVPATVSSD 515
            GCTILLKGAH DELKK+K V+Q  V  A+HL LETSFL D+ A    +P    P TV+  
Sbjct: 610  GCTILLKGAHEDELKKVKHVIQYGVFAAYHLALETSFLADEGASIHELPL-QTPITVALP 669

Query: 516  PQ--------SPLEPCSPNAQGASNGSNLEGEPYNA---AIFSGLSSISDSLKRVMGESF 575
             +        S +   + ++   S  + L GEP+ A      +  SS +    ++ G   
Sbjct: 670  DKPSMVNRSISTIPGFTVSSAEKSPTTELRGEPHKANGDLTGNFTSSKTHFQGKLDGNDR 729

Query: 576  FLASPCQSFSSYFGHGKDLSGLVAKSDS-IPSTPPEADQFDVEVRGSSDEENSI----HE 635
               S     +    + K    + +K D  +P+       F VE      ++ S+     E
Sbjct: 730  IDPSERLLHNLDTVYCKPPETITSKDDGLVPTLESRQLSFHVEEPSVQKDQWSVLSGATE 789

Query: 636  QFVPHQSTFD------EGFGFHETAPNYSEDKLPKKTPFNSQSILVLMSSRNALKGTMCE 695
            Q      T D      + F   E   +   D  P  +  + QSILV +S+R   KG++CE
Sbjct: 790  QVTDGGYTNDSAVIGNQNFNRQEQMESSKGDFHPSAS--DHQSILVSLSTRCVWKGSVCE 849

Query: 696  QSHFSHIVFYKNFDAPLGKFLQENLLNQKNVCTVCGELPEAHYYYYAHSRKQLCIQVKQL 755
            ++H   I +Y +FD PLG+FL++NL +Q   C  C    EAH + Y H +  L I VK+L
Sbjct: 850  RAHLLRIKYYGSFDKPLGRFLRDNLFDQDQCCPSCTMPAEAHIHCYTHRQGSLTISVKKL 909

Query: 756  PVDKVLPGETEGKLWMWSRCSKCKSKGGPSKSTKRVLISTAARGLSFGKFLELCFSDCTL 815
            P  ++LPG+ EGK+WMW RC KC    G   +T+R+++S AA GLSFGKFLEL FS+   
Sbjct: 910  P--ELLPGQREGKIWMWHRCLKCPRINGFPPATRRIVMSDAAWGLSFGKFLELSFSNHAA 969

Query: 816  PSKSSVCGHSLFGDFLYFFGLGNMVAMFRYTEVAIYTVSMPPQKLEFNNSMRQGHLITET 875
             S+ + CGHSL  D L F+G G MVA FRY  + IY V++PP KL FN    Q  L  E+
Sbjct: 970  ASRVANCGHSLHRDCLRFYGFGRMVACFRYASINIYAVTLPPAKLYFNYE-NQEWLQKES 1029

Query: 876  ENVYEKGMILFTEIAQFLKKIISE-----RKSSTVNDFSL-LEE---MLNEERSEFELNI 935
            + V +K  +LF E+ + L +I ++      K ST N   L LEE   +L + + E++ ++
Sbjct: 1030 KEVIKKAEVLFNEVQEALSQISAKTMGAGSKGSTPNKIKLSLEELAGLLEQRKKEYKDSL 1089

Query: 936  QSSL--TKKGNPDFPFHKYLSLNRLLWELLLESCIWDKRLQLLTSLGVTINSGTSENVEP 995
            Q  L   K G P       L +N+L   ++ +S  WD+ L    ++         +N  P
Sbjct: 1090 QQMLNVVKDGQPTIDI---LLINKLRRLIIFDSYAWDECLAGAANMVRNNYLEAPKNSAP 1149

Query: 996  EPV-RHEGLESIAQTDDRNVQQDVSVDENAL------------------HSKEIAVEHLD 1055
            + + R+  LE ++    +++   V++  ++L                   S + A+    
Sbjct: 1150 KVMGRNVSLEKLSDEKVKSIPTHVAICNDSLLQDADYETCLNQGKSFADTSGKFAIPEDV 1209

Query: 1056 G---------ESDGDELGLPSATEVTEI-------------PIMDDLSPKQLSRQGSLLN 1115
            G         E D  E G  +  E +++              + D L    +  Q +  N
Sbjct: 1210 GSDRPPDCRMEFDPSEGGKDNFVESSQVVKPAHTESQFQATDLSDTLDAAWIGEQTTSEN 1269

Query: 1116 GFNR---RHSDDEDPQAG--RVLSSGDIPSSTGNLTSDKLFWTPFSEIQQMRMRDIQRSY 1175
            G  R   R +     Q    R+L S    +  G  T+D+   T            + ++Y
Sbjct: 1270 GIFRPPSRAASTNGTQIPDLRLLGSESELNFKGGPTNDE-HTTQVQLPSPSFYYSLNKNY 1329

Query: 1176 FPELKS--ISNYTPKLLPTAHEFINKEGQKLHIFLSNEKFIVSDYEGELSSIVACVLALL 1235
                +   ++   P  + +  E   + G +L + L     ++  Y+ E +SI+A  L   
Sbjct: 1330 SLNSRKHIMAEDRPVYVSSYRELEWRSGARLLLPLGCNDLVLPVYDDEPTSIIAYALT-- 1389

Query: 1236 KDLSLETDLHNEDSKGEGLNKVPSSGSSDSDSSVTSEEHQFSSFDRLNLLDSLLPKTFKR 1295
                 +  +   D   + L+   S    DS + ++       S D    L S   +  + 
Sbjct: 1390 -SSEYKAQMSGSDKSRDRLDSGGSFSLFDSVNLLSLNSLSDLSVDMSRSLSSADEQVSQL 1449

Query: 1296 AEHEGVIKSL------------AKGKYLVNCPYFNQFRDLRRRCCPSELHYIASLSRCIN 1355
                  +K L             K KY V C Y  +F  LR  CCPSE  +I SL RC  
Sbjct: 1450 LHSSLYLKDLHARISFTDEGPPGKVKYSVTCYYAKEFEALRMICCPSETDFIRSLGRCRK 1509

Query: 1356 WNAKGGKSKSFFAKTLDDRFIIKEIKRTEYESFMKFAPDYFKYINESFDMGNQTCLAKVL 1415
            W A+GGKS  FFAK+LDDRFIIK++ +TE ESF+KF P YFKY+ ES    + T LAK+L
Sbjct: 1510 WGAQGGKSNVFFAKSLDDRFIIKQVTKTELESFIKFGPAYFKYLTESISTKSPTSLAKIL 1569

Query: 1416 GIYQVTVREPKSGKEVRHELMVMENLSFGRNIIRQYDLKGALHARYNPATNDFGEVLLDQ 1475
            GIYQV+ +  K GKE + +++VMENL F RN  R YDLKG+  ARYNP T+    VLLDQ
Sbjct: 1570 GIYQVSSKHLKGGKEFKMDVLVMENLLFKRNFTRLYDLKGSTRARYNPDTSGSNTVLLDQ 1629

Query: 1476 NFVNDMNSSPLYVNNQAKRRLQRAIWNDTAFLNSMNVMDYSLLVGVDAKKKELVCGIIDY 1490
            N V  M +SP++V ++AKR L+RA+WNDT+FL S++VMDYSLLVGVD ++ ELV GIID+
Sbjct: 1630 NLVEAMPTSPIFVGSKAKRLLERAVWNDTSFLASIHVMDYSLLVGVDEERNELVLGIIDF 1689

BLAST of CmaCh09G011720 vs. TAIR 10
Match: AT4G33240.3 (1-phosphatidylinositol-4-phosphate 5-kinases;zinc ion binding;1-phosphatidylinositol-3-phosphate 5-kinases )

HSP 1 Score: 738.0 bits (1904), Expect = 1.5e-212
Identity = 520/1432 (36.31%), Postives = 751/1432 (52.44%), Query Frame = 0

Query: 156  LWDPPEPEDPQDHVEGGMGYDDDDDDEFDDSTEWNTSSSFSRSVDEASISYRIKEQKQRA 215
            LW PPEPE+ +D  E  +  DD D+ +  D      S+SF+         +  K++   A
Sbjct: 311  LWLPPEPENEEDEREAVLSDDDGDEGDRGDWGYLRPSNSFNEK------DFHSKDKSSGA 370

Query: 216  MQQIMNGKFKVFIGHLLKFVGVASSGEG-SENWVDIVSSLSWEAATFLKP-VVNGKAVDP 275
            M+ ++ G F+  +  LL+   +    EG  E W+DI++SLSWEAAT LKP       +DP
Sbjct: 371  MKNVVEGHFRALVAQLLEVDNLPMVNEGDEEGWLDIITSLSWEAATLLKPDTSKSGGMDP 430

Query: 276  EAHVKVKCIATGTRSQSQFVKGMVFKKHAAHKHMPTHCKNPKLILIEGMLGEAPIS-RLS 335
              +VKVKCI  G RS+S  VKG+V KK+ AH+ M +  + P+L+++ G L    IS +LS
Sbjct: 431  GGYVKVKCIPCGRRSESMVVKGVVCKKNVAHRRMTSKIEKPRLLILGGALEYQRISNQLS 490

Query: 336  SFNS-MDQENDFTKHVIEMIEVCTANVILVEKTTSRVIQEAMLKKGMTLVLDMKLHRLER 395
            SF++ + QE D  K  +  I+    +++LVEK+ SR  QE +L K ++LVL++K   LER
Sbjct: 491  SFDTLLQQEMDHLKMAVAKIDSHNPDILLVEKSVSRFAQEYLLAKDISLVLNIKRSLLER 550

Query: 396  IALCTGSPILTS-ETLMSQNTRQCDAVYFEKIVEEHAGLLEGGKRPTKTLMFIEGCPTRL 455
            I+ CTG+ I+ S + L S     CD  + EK VE H    +  K+  KTLMF +GCP  L
Sbjct: 551  ISRCTGAQIVPSIDQLTSPKLGYCDLFHVEKFVETHVSPCQVAKKMAKTLMFFDGCPKPL 610

Query: 456  GCTILLKGAHSDELKKIKVVVQIAVVMAFHLMLETSFLVDQRAMFATIPFGGVPATVSSD 515
            GCTILLKGAH DELKK+K V+Q  V  A+HL LETSFL D+ A    +P    P TV+  
Sbjct: 611  GCTILLKGAHEDELKKVKHVIQYGVFAAYHLALETSFLADEGASIHELPL-QTPITVALP 670

Query: 516  PQ--------SPLEPCSPNAQGASNGSNLEGEPYNA---AIFSGLSSISDSLKRVMGESF 575
             +        S +   + ++   S  + L GEP+ A      +  SS +    ++ G   
Sbjct: 671  DKPSMVNRSISTIPGFTVSSAEKSPTTELRGEPHKANGDLTGNFTSSKTHFQGKLDGNDR 730

Query: 576  FLASPCQSFSSYFGHGKDLSGLVAKSDS-IPSTPPEADQFDVEVRGSSDEENSI----HE 635
               S     +    + K    + +K D  +P+       F VE      ++ S+     E
Sbjct: 731  IDPSERLLHNLDTVYCKPPETITSKDDGLVPTLESRQLSFHVEEPSVQKDQWSVLSGATE 790

Query: 636  QFVPHQSTFD------EGFGFHETAPNYSEDKLPKKTPFNSQSILVLMSSRNALKGTMCE 695
            Q      T D      + F   E   +   D  P  +  + QSILV +S+R   KG++CE
Sbjct: 791  QVTDGGYTNDSAVIGNQNFNRQEQMESSKGDFHPSAS--DHQSILVSLSTRCVWKGSVCE 850

Query: 696  QSHFSHIVFYKNFDAPLGKFLQENLLNQKNVCTVCGELPEAHYYYYAHSRKQLCIQVKQL 755
            ++H   I +Y +FD PLG+FL++NL +Q   C  C    EAH + Y H +  L I VK+L
Sbjct: 851  RAHLLRIKYYGSFDKPLGRFLRDNLFDQDQCCPSCTMPAEAHIHCYTHRQGSLTISVKKL 910

Query: 756  PVDKVLPGETEGKLWMWSRCSKCKSKGGPSKSTKRVLISTAARGLSFGKFLELCFSDCTL 815
            P  ++LPG+ EGK+WMW RC KC    G   +T+R+++S AA GLSFGKFLEL FS+   
Sbjct: 911  P--ELLPGQREGKIWMWHRCLKCPRINGFPPATRRIVMSDAAWGLSFGKFLELSFSNHAA 970

Query: 816  PSKSSVCGHSLFGDFLYFFGLGNMVAMFRYTEVAIYTVSMPPQKLEFNNSMRQGHLITET 875
             S+ + CGHSL  D L F+G G MVA FRY  + IY V++PP KL FN    Q  L  E+
Sbjct: 971  ASRVANCGHSLHRDCLRFYGFGRMVACFRYASINIYAVTLPPAKLYFNYE-NQEWLQKES 1030

Query: 876  ENVYEKGMILFTEIAQFLKKIISE-----RKSSTVNDFSL-LEEM--LNEERSEFELNIQ 935
            + V +K  +LF E+ + L +I ++      K ST N   L LEE+  L E+R + E    
Sbjct: 1031 KEVIKKAEVLFNEVQEALSQISAKTMGAGSKGSTPNKIKLSLEELAGLLEQRKK-EYKQM 1090

Query: 936  SSLTKKGNPDFPFHKYLSLNRLLWELLLESCIWDKRLQLLTSLGVTINSGTSENVEPEPV 995
             ++ K G P       L +N+L   ++ +S  WD+ L    ++         +N  P+ +
Sbjct: 1091 LNVVKDGQPTIDI---LLINKLRRLIIFDSYAWDECLAGAANMVRNNYLEAPKNSAPKVM 1150

Query: 996  -RHEGLESIAQTDDRNVQQDVSVDENAL------------------HSKEIAVEHLDG-- 1055
             R+  LE ++    +++   V++  ++L                   S + A+    G  
Sbjct: 1151 GRNVSLEKLSDEKVKSIPTHVAICNDSLLQDADYETCLNQGKSFADTSGKFAIPEDVGSD 1210

Query: 1056 -------ESDGDELGLPSATEVTEI-------------PIMDDLSPKQLSRQGSLLNGFN 1115
                   E D  E G  +  E +++              + D L    +  Q +  NG  
Sbjct: 1211 RPPDCRMEFDPSEGGKDNFVESSQVVKPAHTESQFQATDLSDTLDAAWIGEQTTSENGIF 1270

Query: 1116 R---RHSDDEDPQAG--RVLSSGDIPSSTGNLTSDKLFWTPFSEIQQMRMRDIQRSYFPE 1175
            R   R +     Q    R+L S    +  G  T+D+   T            + ++Y   
Sbjct: 1271 RPPSRAASTNGTQIPDLRLLGSESELNFKGGPTNDE-HTTQVQLPSPSFYYSLNKNYSLN 1330

Query: 1176 LKS--ISNYTPKLLPTAHEFINKEGQKLHIFLSNEKFIVSDYEGELSSIVACVLALLKDL 1235
             +   ++   P  + +  E   + G +L + L     ++  Y+ E +SI+A  L      
Sbjct: 1331 SRKHIMAEDRPVYVSSYRELEWRSGARLLLPLGCNDLVLPVYDDEPTSIIAYALT---SS 1390

Query: 1236 SLETDLHNEDSKGEGLNKVPSSGSSDSDSSVTSEEHQFSSFDRLNLLDSLLPKTFKRAEH 1295
              +  +   D   + L+   S    DS + ++       S D    L S   +  +    
Sbjct: 1391 EYKAQMSGSDKSRDRLDSGGSFSLFDSVNLLSLNSLSDLSVDMSRSLSSADEQVSQLLHS 1450

Query: 1296 EGVIKSL------------AKGKYLVNCPYFNQFRDLRRRCCPSELHYIASLSRCINWNA 1355
               +K L             K KY V C Y  +F  LR  CCPSE  +I SL RC  W A
Sbjct: 1451 SLYLKDLHARISFTDEGPPGKVKYSVTCYYAKEFEALRMICCPSETDFIRSLGRCRKWGA 1510

Query: 1356 KGGKSKSFFAKTLDDRFIIKEIKRTEYESFMKFAPDYFKYINESFDMGNQTCLAKVLGIY 1415
            +GGKS  FFAK+LDDRFIIK++ +TE ESF+KF P YFKY+ ES    + T LAK+LGIY
Sbjct: 1511 QGGKSNVFFAKSLDDRFIIKQVTKTELESFIKFGPAYFKYLTESISTKSPTSLAKILGIY 1570

Query: 1416 QVTVREPKSGKEVRHELMVMENLSFGRNIIRQYDLKGALHARYNPATNDFGEVLLDQNFV 1475
            QV+ +  K GKE + +++VMENL F RN  R YDLKG+  ARYNP T+    VLLDQN V
Sbjct: 1571 QVSSKHLKGGKEFKMDVLVMENLLFKRNFTRLYDLKGSTRARYNPDTSGSNTVLLDQNLV 1630

Query: 1476 NDMNSSPLYVNNQAKRRLQRAIWNDTAFLNSMNVMDYSLLVGVDAKKKELVCGIIDYLRQ 1490
              M +SP++V ++AKR L+RA+WNDT+FL S++VMDYSLLVGVD ++ ELV GIID++RQ
Sbjct: 1631 EAMPTSPIFVGSKAKRLLERAVWNDTSFLASIHVMDYSLLVGVDEERNELVLGIIDFMRQ 1690