CmaCh09G008710 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh09G008710
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Descriptionnon-canonical poly(A) RNA polymerase PAPD5
LocationCma_Chr09: 4272857 .. 4278408 (+)
RNA-Seq ExpressionCmaCh09G008710
SyntenyCmaCh09G008710
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
CGAATTACTAAAACCCCTTGCTGCTGCATCTCCCTGTCTCTGTGGCCTTCAATTTCAATGGAGGAAGAGACTGTTCAACAGTATCTGTACGATACTCTAAGCCCTCTTTCATTCTCCGCCGTCGCTACCACCGGAAGCGAGGAGCAACTCTCGCCTTCCGATAACCTCGAGCCCTACGCCGTTTTCCGCAACGAGATCTCTCTCTCTACTTCTCATTCTGCTCCGGCCGAGACTGCAGCAACCGAGTTTTTTGCCCTTGATGTTGCTGCTGACAACGTGGAGGAAATTTCGGGGTTTTCTACTCCTTTGGCGGTTACTTCGGTTCAGGAAACTGAGCCTAGGACGCCGGAATGTGAGGATCAGTCGATGCTTGAAAGTGGGTGGTTCCGTGGCAATGGTGGATTCAAGAGCCCTATGCTACAGTTGCATAAAGGTAATCGGATTAGTTTGTTGCTCTTGAGTCGGTAGAAGTTTATTTCAAACGGTTACACTCATCTTTGAATTATCTCCTCGACGACAGAAGTTTCAGTTATCGAGTTAAAATAGAACGTGGGTCTTGTTGTTTGTTTATTTGAACTTCTCATCGTTTTCAATGGACTGGCATTTAACTGCCACGGCAGCCTCTTCGCCGTCCCGAGCCTAAAATCCGGTCATCAGTCGTTCCACTCCTTTTTTTGTTCGGCTCAACCTTTTTCTCAGAAGGTGGAATTAAAAAGTAGGGATTCGGGTTGGAAGATTTTTAAAAGAAGATTTTTTGCTTTTGTTTTGGATAGATAAATGTAATTATCATCCACATTTTCTTCCTCAAGCTACTCGTAATATAACATGGTTAATTGGTTACACTATCTGAATTCCACTGCGTTTGTTCTCTTAACTCTTTCCTTTGTATCTATCTATAAAATTTCAAGGTGCTGGTTCGTTTACTTAATCTGCAAATTGTATACGAAGCTAGTTTCTCCTCATACCAACAATCCAACCGTGTCATTACGTTCATCTCATTCCATGGTTTACGATGCGGCCCTTGATACCCACATCCATAATTTGTTATCATGCTGTACACATCATTGTTGTTCTGAGTTCATAACTTTTGATTACATGCAGAAATAGTTGATTTTTGTGAGTTTCTATCTCCAACTGAAGAAGAACGTGTTGCACGAGACAGTGCAGTTGAAAGGGTTTTTAGTGTAGTCAAACACATATGGCCTCACTGTAAGGTATGATTGATGCCCCCTATAATTTCGTGTCCCATGCTTGATTTCAAGAGAATGGAATATTTTATTTATTTATTCCTTTGCGTTTTATAGGTTGAAGTTTTTGGATCGTTTCAGACAGGGCTTTATCTTCCAACCAGTGACATCGATGTGAGTTTCTAGATCCATGTTTTAGATGTTTCATGATTTTCAACTCTTATTATAATATGCTTTACAACTACCCGTTTTTCTTGGCCGTTGTTACTGACTGGATCATTGGAAAGAAATTAGAAAGAGTTGGAAATTTCTTGACAAGTGCAAAGAGGTTGAAAGAGGATCTGCCGAGAATTCTAATATGAGATCCAAGTAAATGATATAGTTGATCCCTAATAGAGTAGAGACAGCTGCACAACAATATGGTGGATTTGGGAGTGATTCCAATTCATTTTTTGCTTCTTCATGATTCTGCATTTTTTATTGTTAAATGCAACTACGTGAATGATACTCGCTAAAAATGTAAGGATTATGAAATATGTGGCATATGAACAGTTAGTTTAGGTCAAGTGAAGTCAATGTGAGTCTGTGATATGGTTTGGTCCTCATCATATGTAACATTTTTCTAAGCTACTACATTCTTCTCTGGAACTTGCGGTCTGCAATTGCATATTTAACAGGATAGTGGCAGCTTTTATTTGTAATTGGGCTGTCGGTCTTTATATACATAGTTTTTCTTTTTTGTCCTTGATTAGTGATATTTCATAATGGATTGACTATGGAAAAATTGGTTATTATTTAGAATCAAGTGGTCGATCTTATTGGATGCAATTTTTTTTTTATATTTCAGTAAATTTATGATTATAATTTATAACTCGTTTTTGCAGGTTGTTATTCTGGATTCAGGCATCCCAAAACCACAACTTGGTTTGCAAGCTCTTTCCAGGGCTTTATCACAAAAGGGAATAGCAAAAAAGATTCAGGTATCTTTTAATGATAGTGATTGCTTTGGTCGGGATTGGATCAGGATCTTCATTGACTTTATTTTTGGAATCTCCCATGTTATCATTGGATCAGGTTATTGGAAAGGCTCGTGTGCCAATAATTAAGTTTGTTGAAAAGCAAAGTGGTATATCTTTTGATATAAGGTATAATGTTAATTTTTGGGACTACTATTGATATTTATAAATTTGTTTAAAAAAAATTCTTCTTTCCACGAAGAAAAGTAAAAGTGAAGTTAAAACAGGATTTGCACTAAAAAGCATCCATAAAACAATTACTGTATCCAGAAGAGTGCACAGAGCTCCATTCACTGGTTAAGTTTTTTTTTTCAGTATTGAATATGCTTTTGCAACTGAAAACTGATGGAAAACTAAACTTTCATTGAGATAATATTGATAATATCTATTGCACCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCNCCCCCCCCCCCCCCCCCACACACACACACACACACAAATATACAGCTAAAGTGATTAGAGAATAGTAACTCAAAAGGAGGAATGTAATGATGGAAAAGCCCATACTAGTCTAAAAATTGACATCTTATCTCTTGCTTTACAGTTTTGACGTGCAGAATGGACCAAAAGCTGCTGATTTTATAAAGGTTTGGTGCTGTACTTCAATCTTTCGTTATGATGGCCTTCCTAGCTCAATTATTGCAGTGTGTATTTTATTGTTGTGATCTTTATTAGGAGGTGACTGCTTGATCTTCAAATTATGTTTGTGAGGTTTTCTCAAAATGACTTCTTTCAACAAATGTTACAAGTTCTGTGATAATTTGCTGTTGTATATTAATTTTTCTTGGTTCTTACAGAATAATATTAATTATTTTTTCTTGATGTCTTTTGCCTATTTCTTGTTTGGATTTCAGTGTATTCTTCCACCGTTTGTAATTTCAACAGAACATGCATGTTTTTAGTCATTGGTATTTGAGGAATATTTTGATTGATTATGGAATTTGATTGCACATAATTGAAAATAAGTTTGAGTTCGAATACTCAAACGAAATCTGACTTTCACTTCCCTTATTGTTAGAAAAATGGATTTGAATTCTATGGTTATTGATTGCTAATGGAATGTGTACATATAGTTGATTAGAAGCATGGCTTGGGATATTTGCATGAAAACCTCCGTACACATGGATTTGAAATGTACGGTTTATCTGCTTATTGCTATTACTTTCCCCCTTGCAGGGTGCAATCAGTAAGTGGCCTCCTTTGCGTCCTCTGTGTTTGATCTTAAAGGTGTTTTTGCAGCAGAGAGAACTGAATGAGGTATGTTTCTAACTGTGGATTTCAATTCTTATTGTAGTTGTCATATCGTGTATGTTAGTCGATCCATTATCTTTAAATTTTCTGTGTCTTAATTCAAGTTTCTGTATGGTGTGCAGGTATATTCTGGTGGACTAGGTTCTTATGCACTTCTTACTATGCTTATGGCCATGTTGCAGGTTAGTGTATTTGAACTTTTACATTTCGACTGTATAGGTTATTTTTCCTACCAAAGAACATATGTAACTTTTTTTATTTTTGTCCTTTCAAAGGAGCATTGAGACCGGCCAAATTACATTTGCTTCTGAAGTAATATTTGATTGTGCTAATATTTGTCTGTCAGTGCATTGGTTTCTGGCCAAGTGTATTTTAGGAATTGAATTTAAGGCATTTCCATTTAATCGTGGCAGATTATTCTTTAATTTGGTTTTATTTATTGCACAGAGTTTCAACGTGTCCCACTCTTCTCTTGAACACAATCTGGGAGTCCTTTTGGTATGTGTATTTTATTGTTCGAAGTACCTACTAGCATTGTAGTGAAAAATATTTTCTCAGATGTGAAAATAGTGCGAAAACATAATTTTTTTTTCGTAGTTCTCGTGTAGCTATTGCTGCTATGAGTAGTCTTGGTACTATATTCGTTTAGCAAGCCAATCTATTCTCGGAAATCGCCTCTGGGTCTTGTGCTTTATGGAAGCTGGCTTTATTAGACCTACTCTTTTCCTCTTTCTAATTACTCTTCTACTGCCAGAAGTCTATCCTTTGCAATTTTTTCTGTATACTTAGAAACAGAAGTGGAGGAGAAGGATCTCTTTTTTATATTGTCTTAATTTTGAAAATCAGGTACATTTCTTTGATTTTTATGGTCGCAAATTGAACACTTCTGATGTTGGTGTATCTTGCAATACGGGAGGCATTTTTTTCTCGAAGAGTAACAGAGGGTAAACCACATAAAAGATTATATGATGAATATAAATTCCATTCTCATTTTCAGGCAACACATTTCATTACGTCCTATTTTGGCTTCTCCCTAATTTTTTCTTTTATTTGGTACTGTTGCAGATTCATGACAAAAGGACGCCCATTTCTTCTTTCCATTGAGGATCCTCAGGTTAATTTCTATTTTCGACATACTAACAGGGAGGTTGTTTGAGCAGTTTGTGTATAATTGTTGTAGAATATAGTATCTCAACTCCCTTCGAATTCGACATAAACTTGTTTTGTCTGGACCAAAGGATGTCTGATTTGCTGTGCTAGATGTAAATCTTTGAAATAGGCCTCTGAGACGTTACGGTTCGACCATGTTCTCGTTACCTCAAGATGAATTGCTTGATGAATATAATTTCACTTTGTTTTGATCTCTATTTTTTAAAAAAATTGATAACTTGATGATTGAGCATTCCTTGCTTTAAGTAATGTAAAAGGCTGTAAGATATAGGTCTCCATTTCCTTCTTATAAGTTCTTTCTCTTATGTGATTTATTGGTGTAATCCAGGCACCAGATAATGACATTGGCAAGAATTCCTACAATTACTTCCAGGTTTGTTCTTCAATCATGTATGTCGATTAACTTTCTTTTCGACTCCGACTTTGTTTCTGATATCTACCATGGTTGCAGATAAGATCTGCATTTGCAATGGCCTTTTCAATCTTGACAAATGTTAAGACCATTTTAGGTCTTGGTCCAAACCGAAGTATTCTAGGCACAATAATCCGACCCGATCCAGTCTTGTTGAAGCGAAAAGGTGGCAGAGATGGAGAAGTGACCTTTACTTCTTTACTCCCTGGAGCAGGGGAACCTATACAACAACAACCAGAATATGGGGATGGTCAGGAGATGCTGTGCAATTGGCAGTTCGGCGACGATGAACCATTGCCTCGAGGGAACGACGCATCCAGGAATTTGGATACGCCGTCCTCTAAAAAAAAGAGAAAATCTCGCTGCGAGAAGTCGAGAAATAAGGACAAAGAAAACCATGGGAGTAGTAAGCGCAGCAAAGAGAAGAGTTCGAGGAAGAAACGATGGAGAGAGAACGGGAGTGATGGCAGAAGGTCTCCTTGGAAAAGATAA

mRNA sequence

CGAATTACTAAAACCCCTTGCTGCTGCATCTCCCTGTCTCTGTGGCCTTCAATTTCAATGGAGGAAGAGACTGTTCAACAGTATCTGTACGATACTCTAAGCCCTCTTTCATTCTCCGCCGTCGCTACCACCGGAAGCGAGGAGCAACTCTCGCCTTCCGATAACCTCGAGCCCTACGCCGTTTTCCGCAACGAGATCTCTCTCTCTACTTCTCATTCTGCTCCGGCCGAGACTGCAGCAACCGAGTTTTTTGCCCTTGATGTTGCTGCTGACAACGTGGAGGAAATTTCGGGGTTTTCTACTCCTTTGGCGGTTACTTCGGTTCAGGAAACTGAGCCTAGGACGCCGGAATGTGAGGATCAGTCGATGCTTGAAAGTGGGTGGTTCCGTGGCAATGGTGGATTCAAGAGCCCTATGCTACAGTTGCATAAAGAAATAGTTGATTTTTGTGAGTTTCTATCTCCAACTGAAGAAGAACGTGTTGCACGAGACAGTGCAGTTGAAAGGGTTTTTAGTGTAGTCAAACACATATGGCCTCACTGTAAGGTTGAAGTTTTTGGATCGTTTCAGACAGGGCTTTATCTTCCAACCAGTGACATCGATGTTGTTATTCTGGATTCAGGCATCCCAAAACCACAACTTGGTTTGCAAGCTCTTTCCAGGGCTTTATCACAAAAGGGAATAGCAAAAAAGATTCAGGTTATTGGAAAGGCTCGTGTGCCAATAATTAAGTTTGTTGAAAAGCAAAGTGGTATATCTTTTGATATAAGTTTTGACGTGCAGAATGGACCAAAAGCTGCTGATTTTATAAAGGGTGCAATCAGTAAGTGGCCTCCTTTGCGTCCTCTGTGTTTGATCTTAAAGGTGTTTTTGCAGCAGAGAGAACTGAATGAGGTATATTCTGGTGGACTAGGTTCTTATGCACTTCTTACTATGCTTATGGCCATGTTGCAGAGTTTCAACGTGTCCCACTCTTCTCTTGAACACAATCTGGGAGTCCTTTTGGTACATTTCTTTGATTTTTATGGTCGCAAATTGAACACTTCTGATGTTGGTGTATCTTGCAATACGGGAGGCATTTTTTTCTCGAAGAGTAACAGAGGATTCATGACAAAAGGACGCCCATTTCTTCTTTCCATTGAGGATCCTCAGGCACCAGATAATGACATTGGCAAGAATTCCTACAATTACTTCCAGATAAGATCTGCATTTGCAATGGCCTTTTCAATCTTGACAAATGTTAAGACCATTTTAGGTCTTGGTCCAAACCGAAGTATTCTAGGCACAATAATCCGACCCGATCCAGTCTTGTTGAAGCGAAAAGGTGGCAGAGATGGAGAAGTGACCTTTACTTCTTTACTCCCTGGAGCAGGGGAACCTATACAACAACAACCAGAATATGGGGATGGTCAGGAGATGCTGTGCAATTGGCAGTTCGGCGACGATGAACCATTGCCTCGAGGGAACGACGCATCCAGGAATTTGGATACGCCGTCCTCTAAAAAAAAGAGAAAATCTCGCTGCGAGAAGTCGAGAAATAAGGACAAAGAAAACCATGGGAGTAGTAAGCGCAGCAAAGAGAAGAGTTCGAGGAAGAAACGATGGAGAGAGAACGGGAGTGATGGCAGAAGGTCTCCTTGGAAAAGATAA

Coding sequence (CDS)

ATGGAGGAAGAGACTGTTCAACAGTATCTGTACGATACTCTAAGCCCTCTTTCATTCTCCGCCGTCGCTACCACCGGAAGCGAGGAGCAACTCTCGCCTTCCGATAACCTCGAGCCCTACGCCGTTTTCCGCAACGAGATCTCTCTCTCTACTTCTCATTCTGCTCCGGCCGAGACTGCAGCAACCGAGTTTTTTGCCCTTGATGTTGCTGCTGACAACGTGGAGGAAATTTCGGGGTTTTCTACTCCTTTGGCGGTTACTTCGGTTCAGGAAACTGAGCCTAGGACGCCGGAATGTGAGGATCAGTCGATGCTTGAAAGTGGGTGGTTCCGTGGCAATGGTGGATTCAAGAGCCCTATGCTACAGTTGCATAAAGAAATAGTTGATTTTTGTGAGTTTCTATCTCCAACTGAAGAAGAACGTGTTGCACGAGACAGTGCAGTTGAAAGGGTTTTTAGTGTAGTCAAACACATATGGCCTCACTGTAAGGTTGAAGTTTTTGGATCGTTTCAGACAGGGCTTTATCTTCCAACCAGTGACATCGATGTTGTTATTCTGGATTCAGGCATCCCAAAACCACAACTTGGTTTGCAAGCTCTTTCCAGGGCTTTATCACAAAAGGGAATAGCAAAAAAGATTCAGGTTATTGGAAAGGCTCGTGTGCCAATAATTAAGTTTGTTGAAAAGCAAAGTGGTATATCTTTTGATATAAGTTTTGACGTGCAGAATGGACCAAAAGCTGCTGATTTTATAAAGGGTGCAATCAGTAAGTGGCCTCCTTTGCGTCCTCTGTGTTTGATCTTAAAGGTGTTTTTGCAGCAGAGAGAACTGAATGAGGTATATTCTGGTGGACTAGGTTCTTATGCACTTCTTACTATGCTTATGGCCATGTTGCAGAGTTTCAACGTGTCCCACTCTTCTCTTGAACACAATCTGGGAGTCCTTTTGGTACATTTCTTTGATTTTTATGGTCGCAAATTGAACACTTCTGATGTTGGTGTATCTTGCAATACGGGAGGCATTTTTTTCTCGAAGAGTAACAGAGGATTCATGACAAAAGGACGCCCATTTCTTCTTTCCATTGAGGATCCTCAGGCACCAGATAATGACATTGGCAAGAATTCCTACAATTACTTCCAGATAAGATCTGCATTTGCAATGGCCTTTTCAATCTTGACAAATGTTAAGACCATTTTAGGTCTTGGTCCAAACCGAAGTATTCTAGGCACAATAATCCGACCCGATCCAGTCTTGTTGAAGCGAAAAGGTGGCAGAGATGGAGAAGTGACCTTTACTTCTTTACTCCCTGGAGCAGGGGAACCTATACAACAACAACCAGAATATGGGGATGGTCAGGAGATGCTGTGCAATTGGCAGTTCGGCGACGATGAACCATTGCCTCGAGGGAACGACGCATCCAGGAATTTGGATACGCCGTCCTCTAAAAAAAAGAGAAAATCTCGCTGCGAGAAGTCGAGAAATAAGGACAAAGAAAACCATGGGAGTAGTAAGCGCAGCAAAGAGAAGAGTTCGAGGAAGAAACGATGGAGAGAGAACGGGAGTGATGGCAGAAGGTCTCCTTGGAAAAGATAA

Protein sequence

MEEETVQQYLYDTLSPLSFSAVATTGSEEQLSPSDNLEPYAVFRNEISLSTSHSAPAETAATEFFALDVAADNVEEISGFSTPLAVTSVQETEPRTPECEDQSMLESGWFRGNGGFKSPMLQLHKEIVDFCEFLSPTEEERVARDSAVERVFSVVKHIWPHCKVEVFGSFQTGLYLPTSDIDVVILDSGIPKPQLGLQALSRALSQKGIAKKIQVIGKARVPIIKFVEKQSGISFDISFDVQNGPKAADFIKGAISKWPPLRPLCLILKVFLQQRELNEVYSGGLGSYALLTMLMAMLQSFNVSHSSLEHNLGVLLVHFFDFYGRKLNTSDVGVSCNTGGIFFSKSNRGFMTKGRPFLLSIEDPQAPDNDIGKNSYNYFQIRSAFAMAFSILTNVKTILGLGPNRSILGTIIRPDPVLLKRKGGRDGEVTFTSLLPGAGEPIQQQPEYGDGQEMLCNWQFGDDEPLPRGNDASRNLDTPSSKKKRKSRCEKSRNKDKENHGSSKRSKEKSSRKKRWRENGSDGRRSPWKR
Homology
BLAST of CmaCh09G008710 vs. ExPASy Swiss-Prot
Match: Q8NDF8 (Terminal nucleotidyltransferase 4B OS=Homo sapiens OX=9606 GN=TENT4B PE=1 SV=2)

HSP 1 Score: 233.4 bits (594), Expect = 5.9e-60
Identity = 134/314 (42.68%), Postives = 188/314 (59.87%), Query Frame = 0

Query: 109 WFRGNGGFKSPMLQLHKEIVDFCEFLSPTEEERVARDSAVERVFSVVKHIWPHCKVEVFG 168
           W R N  +   ++ LH+EI DF E++SP  EE   R   V R+ SV+K +WP   V++FG
Sbjct: 108 WKRRN--YNQGVVGLHEEISDFYEYMSPRPEEEKMRMEVVNRIESVIKELWPSADVQIFG 167

Query: 169 SFQTGLYLPTSDIDVVILDSGIPKPQLGLQALSRALSQKGIAKK--IQVIGKARVPIIKF 228
           SF+TGLYLPTSDID+V+         L L  L  AL +  +A +  ++V+ KA VPIIK 
Sbjct: 168 SFKTGLYLPTSDIDLVVFGKW---ENLPLWTLEEALRKHKVADEDSVKVLDKATVPIIKL 227

Query: 229 VEKQSGISFDISFDVQNGPKAADFIKGAISKWPPLRPLCLILKVFLQQRELNEVYSGGLG 288
            +  + +  DISF+VQNG +AAD IK    K+P L  L L+LK FL QR+LNEV++GG+G
Sbjct: 228 TDSFTEVKVDISFNVQNGVRAADLIKDFTKKYPVLPYLVLVLKQFLLQRDLNEVFTGGIG 287

Query: 289 SYALLTMLMAMLQSFNVSHSSLEH-NLGVLLVHFFDFYGRKLNTSDVGVSCNTGGIFFSK 348
           SY+L  M ++ LQ      + + + N GVLL+ FF+ YGR  N    G+    GG + +K
Sbjct: 288 SYSLFLMAVSFLQLHPREDACIPNTNYGVLLIEFFELYGRHFNYLKTGIRIKDGGSYVAK 347

Query: 349 S--NRGFMTKGRPFLLSIEDPQAPDNDIGKNSYNYFQIRSAFAMAFSILTN-VKTILGLG 408
               +  +   RP +L IEDP  P ND+G++SY   Q++ AF  A+ +L++ V  I    
Sbjct: 348 DEVQKNMLDGYRPSMLYIEDPLQPGNDVGRSSYGAMQVKQAFDYAYVVLSHAVSPIAKYY 407

Query: 409 PN---RSILGTIIR 414
           PN    SILG IIR
Sbjct: 408 PNNETESILGRIIR 416

BLAST of CmaCh09G008710 vs. ExPASy Swiss-Prot
Match: Q68ED3 (Terminal nucleotidyltransferase 4B OS=Mus musculus OX=10090 GN=Tent4b PE=1 SV=2)

HSP 1 Score: 233.4 bits (594), Expect = 5.9e-60
Identity = 134/314 (42.68%), Postives = 188/314 (59.87%), Query Frame = 0

Query: 109 WFRGNGGFKSPMLQLHKEIVDFCEFLSPTEEERVARDSAVERVFSVVKHIWPHCKVEVFG 168
           W R N  +   ++ LH+EI DF E++SP  EE   R   V R+ SV+K +WP   V++FG
Sbjct: 122 WKRRN--YNQGVVGLHEEISDFYEYMSPRPEEEKMRMEVVSRIESVIKELWPSADVQIFG 181

Query: 169 SFQTGLYLPTSDIDVVILDSGIPKPQLGLQALSRALSQKGIAKK--IQVIGKARVPIIKF 228
           SF+TGLYLPTSDID+V+         L L  L  AL +  +A +  ++V+ KA VPIIK 
Sbjct: 182 SFKTGLYLPTSDIDLVVFGKW---ENLPLWTLEEALRKHKVADEDSVKVLDKATVPIIKL 241

Query: 229 VEKQSGISFDISFDVQNGPKAADFIKGAISKWPPLRPLCLILKVFLQQRELNEVYSGGLG 288
            +  + +  DISF+VQNG +AAD IK    K+P L  L L+LK FL QR+LNEV++GG+G
Sbjct: 242 TDSFTEVKVDISFNVQNGVRAADLIKDFTKKYPVLPYLVLVLKQFLLQRDLNEVFTGGIG 301

Query: 289 SYALLTMLMAMLQSFNVSHSSLEH-NLGVLLVHFFDFYGRKLNTSDVGVSCNTGGIFFSK 348
           SY+L  M ++ LQ      + + + N GVLL+ FF+ YGR  N    G+    GG + +K
Sbjct: 302 SYSLFLMAVSFLQLHPREDACIPNTNYGVLLIEFFELYGRHFNYLKTGIRIKDGGSYVAK 361

Query: 349 S--NRGFMTKGRPFLLSIEDPQAPDNDIGKNSYNYFQIRSAFAMAFSILTN-VKTILGLG 408
               +  +   RP +L IEDP  P ND+G++SY   Q++ AF  A+ +L++ V  I    
Sbjct: 362 DEVQKNMLDGYRPSMLYIEDPLQPGNDVGRSSYGAMQVKQAFDYAYVVLSHAVSPIAKYY 421

Query: 409 PN---RSILGTIIR 414
           PN    SILG IIR
Sbjct: 422 PNNETESILGRIIR 430

BLAST of CmaCh09G008710 vs. ExPASy Swiss-Prot
Match: Q5XG87 (Terminal nucleotidyltransferase 4A OS=Homo sapiens OX=9606 GN=TENT4A PE=1 SV=3)

HSP 1 Score: 233.0 bits (593), Expect = 7.7e-60
Identity = 132/306 (43.14%), Postives = 190/306 (62.09%), Query Frame = 0

Query: 118 SPMLQ-LHKEIVDFCEFLSPTEEERVARDSAVERVFSVVKHIWPHCKVEVFGSFQTGLYL 177
           SP +Q LH+EI+DF  F+SP  EE   R   V+R+ +VVK +WP   V++FGSF TGLYL
Sbjct: 214 SPGIQGLHEEIIDFYNFMSPCPEEAAMRREVVKRIETVVKDLWPTADVQIFGSFSTGLYL 273

Query: 178 PTSDIDVVILDSGIPKPQLGLQALSRALSQKGIAK--KIQVIGKARVPIIKFVEKQSGIS 237
           PTSDID+V+       P   LQ L +AL +  +A+   I+V+ KA VPIIK  ++++ + 
Sbjct: 274 PTSDIDLVVFGKWERPP---LQLLEQALRKHNVAEPCSIKVLDKATVPIIKLTDQETEVK 333

Query: 238 FDISFDVQNGPKAADFIKGAISKWPPLRPLCLILKVFLQQRELNEVYSGGLGSYALLTML 297
            DISF+++ G +AA+FIK  + K+  L  L L+LK FL QR+LNEV++GG+ SY+L+ M 
Sbjct: 334 VDISFNMETGVRAAEFIKNYMKKYSLLPYLILVLKQFLLQRDLNEVFTGGISSYSLILMA 393

Query: 298 MAMLQSF-NVSHSSLEHNLGVLLVHFFDFYGRKLNTSDVGVSCNTGGIFFSKSN-RGFMT 357
           ++ LQ    +     + NLG+LLV FF+ YGR  N    G+    GG + +K      MT
Sbjct: 394 ISFLQLHPRIDARRADENLGMLLVEFFELYGRNFNYLKTGIRIKEGGAYIAKEEIMKAMT 453

Query: 358 KG-RPFLLSIEDPQAPDNDIGKNSYNYFQIRSAFAMAFSILTNVKTILGLG-PNR---SI 414
            G RP +L IEDP  P ND+G++SY   Q++  F  A+ +L++  + L    PNR   S 
Sbjct: 454 SGYRPSMLCIEDPLLPGNDVGRSSYGAMQVKQVFDYAYIVLSHAVSPLARSYPNRDAEST 513

BLAST of CmaCh09G008710 vs. ExPASy Swiss-Prot
Match: Q7KVS9 (Non-canonical poly(A) RNA polymerase protein Trf4-1 OS=Drosophila melanogaster OX=7227 GN=Trf4-1 PE=1 SV=1)

HSP 1 Score: 228.0 bits (580), Expect = 2.5e-58
Identity = 129/314 (41.08%), Postives = 193/314 (61.46%), Query Frame = 0

Query: 109 WFRGNGGFKSPMLQLHKEIVDFCEFLSPTEEERVARDSAVERVFSVVKHIWPHCKVEVFG 168
           W + +  +   ++ LH+EI  F +++ PT  E   R+  V+R+ +VV  IWP   VE+FG
Sbjct: 257 WRKPDYPYGEGVIGLHEEIEHFYQYVLPTPCEHAIRNEVVKRIEAVVHSIWPQAVVEIFG 316

Query: 169 SFQTGLYLPTSDIDVVILDSGIPKPQLGLQALSRALSQKGIAK--KIQVIGKARVPIIKF 228
           SF+TGL+LPTSDID+V+L       +L L+ L   L  +GIA+   ++V+ KA VPIIK 
Sbjct: 317 SFRTGLFLPTSDIDLVVLGLW---EKLPLRTLEFELVSRGIAEACTVRVLDKASVPIIKL 376

Query: 229 VEKQSGISFDISFDVQNGPKAADFIKGAISKWPPLRPLCLILKVFLQQRELNEVYSGGLG 288
            ++++ +  DISF++Q+G ++A+ IK     +P L  L L+LK FL  R+LNEV++GG+ 
Sbjct: 377 TDRETQVKVDISFNMQSGVQSAELIKKFKRDYPVLEKLVLVLKQFLLLRDLNEVFTGGIS 436

Query: 289 SYALLTMLMAMLQSF--NVSHSSLEHNLGVLLVHFFDFYGRKLNTSDVGVSCNTGGIFFS 348
           SY+L+ M ++ LQ     + H +   NLGVLL+ FF+ YGR+ N   +G+S   GG +  
Sbjct: 437 SYSLILMCISFLQMHPRGIYHDTA--NLGVLLLEFFELYGRRFNYMKIGISIKNGGRYMP 496

Query: 349 KS--NRGFMTKGRPFLLSIEDPQAPDNDIGKNSYNYFQIRSAFAMAFSIL---TNVKTIL 408
           K    R  +   RP LL IEDP  P NDIG++SY  FQ++ AF  A+ +L    +   +L
Sbjct: 497 KDELQRDMVDGHRPSLLCIEDPLTPGNDIGRSSYGVFQVQQAFKCAYRVLALAVSPLNLL 556

Query: 409 GLGPN-RSILGTII 413
           G+ P   SILG II
Sbjct: 557 GIDPRVNSILGRII 565

BLAST of CmaCh09G008710 vs. ExPASy Swiss-Prot
Match: Q6PB75 (Terminal nucleotidyltransferase 4A OS=Mus musculus OX=10090 GN=Tent4a PE=2 SV=2)

HSP 1 Score: 215.3 bits (547), Expect = 1.7e-54
Identity = 122/289 (42.21%), Postives = 177/289 (61.25%), Query Frame = 0

Query: 134 LSPTEEERVARDSAVERVFSVVKHIWPHCKVEVFGSFQTGLYLPTSDIDVVILDSGIPKP 193
           +SP  EE   R   V+R+ +VVK +WP   V++FGSF TGLYLPTSDID+V+       P
Sbjct: 1   MSPCPEEAAMRREVVKRIETVVKDLWPTADVQIFGSFSTGLYLPTSDIDLVVFGKWERPP 60

Query: 194 QLGLQALSRALSQKGIAK--KIQVIGKARVPIIKFVEKQSGISFDISFDVQNGPKAADFI 253
              LQ L +AL +  +A+   I+V+ KA VPIIK  ++++ +  DISF+++ G +AA+FI
Sbjct: 61  ---LQLLEQALRKHNVAEPCSIKVLDKATVPIIKLTDQETEVKVDISFNMETGVRAAEFI 120

Query: 254 KGAISKWPPLRPLCLILKVFLQQRELNEVYSGGLGSYALLTMLMAMLQSF-NVSHSSLEH 313
           K  + K+  L  L L+LK FL QR+LNEV++GG+ SY+L+ M ++ LQ    +     + 
Sbjct: 121 KNYMKKYSLLPYLILVLKQFLLQRDLNEVFTGGISSYSLILMAISFLQLHPRIDARRADE 180

Query: 314 NLGVLLVHFFDFYGRKLNTSDVGVSCNTGGIFFSKSN-RGFMTKG-RPFLLSIEDPQAPD 373
           NLG+LLV FF+ YGR  N    G+    GG + +K      MT G RP +L IEDP  P 
Sbjct: 181 NLGMLLVEFFELYGRNFNYLKTGIRIKEGGAYIAKEEIMKAMTSGYRPSMLCIEDPLLPG 240

Query: 374 NDIGKNSYNYFQIRSAFAMAFSILTNVKTILGLG-PNR---SILGTIIR 414
           ND+G++SY   Q++  F  A+ +L++  + L    PNR   S LG II+
Sbjct: 241 NDVGRSSYGAMQVKQVFDYAYIVLSHAVSPLARSYPNRDSESTLGRIIK 286

BLAST of CmaCh09G008710 vs. ExPASy TrEMBL
Match: A0A6J1IMA1 (non-canonical poly(A) RNA polymerase PAPD5 OS=Cucurbita maxima OX=3661 GN=LOC111476864 PE=4 SV=1)

HSP 1 Score: 1042.7 bits (2695), Expect = 5.1e-301
Identity = 530/530 (100.00%), Postives = 530/530 (100.00%), Query Frame = 0

Query: 1   MEEETVQQYLYDTLSPLSFSAVATTGSEEQLSPSDNLEPYAVFRNEISLSTSHSAPAETA 60
           MEEETVQQYLYDTLSPLSFSAVATTGSEEQLSPSDNLEPYAVFRNEISLSTSHSAPAETA
Sbjct: 1   MEEETVQQYLYDTLSPLSFSAVATTGSEEQLSPSDNLEPYAVFRNEISLSTSHSAPAETA 60

Query: 61  ATEFFALDVAADNVEEISGFSTPLAVTSVQETEPRTPECEDQSMLESGWFRGNGGFKSPM 120
           ATEFFALDVAADNVEEISGFSTPLAVTSVQETEPRTPECEDQSMLESGWFRGNGGFKSPM
Sbjct: 61  ATEFFALDVAADNVEEISGFSTPLAVTSVQETEPRTPECEDQSMLESGWFRGNGGFKSPM 120

Query: 121 LQLHKEIVDFCEFLSPTEEERVARDSAVERVFSVVKHIWPHCKVEVFGSFQTGLYLPTSD 180
           LQLHKEIVDFCEFLSPTEEERVARDSAVERVFSVVKHIWPHCKVEVFGSFQTGLYLPTSD
Sbjct: 121 LQLHKEIVDFCEFLSPTEEERVARDSAVERVFSVVKHIWPHCKVEVFGSFQTGLYLPTSD 180

Query: 181 IDVVILDSGIPKPQLGLQALSRALSQKGIAKKIQVIGKARVPIIKFVEKQSGISFDISFD 240
           IDVVILDSGIPKPQLGLQALSRALSQKGIAKKIQVIGKARVPIIKFVEKQSGISFDISFD
Sbjct: 181 IDVVILDSGIPKPQLGLQALSRALSQKGIAKKIQVIGKARVPIIKFVEKQSGISFDISFD 240

Query: 241 VQNGPKAADFIKGAISKWPPLRPLCLILKVFLQQRELNEVYSGGLGSYALLTMLMAMLQS 300
           VQNGPKAADFIKGAISKWPPLRPLCLILKVFLQQRELNEVYSGGLGSYALLTMLMAMLQS
Sbjct: 241 VQNGPKAADFIKGAISKWPPLRPLCLILKVFLQQRELNEVYSGGLGSYALLTMLMAMLQS 300

Query: 301 FNVSHSSLEHNLGVLLVHFFDFYGRKLNTSDVGVSCNTGGIFFSKSNRGFMTKGRPFLLS 360
           FNVSHSSLEHNLGVLLVHFFDFYGRKLNTSDVGVSCNTGGIFFSKSNRGFMTKGRPFLLS
Sbjct: 301 FNVSHSSLEHNLGVLLVHFFDFYGRKLNTSDVGVSCNTGGIFFSKSNRGFMTKGRPFLLS 360

Query: 361 IEDPQAPDNDIGKNSYNYFQIRSAFAMAFSILTNVKTILGLGPNRSILGTIIRPDPVLLK 420
           IEDPQAPDNDIGKNSYNYFQIRSAFAMAFSILTNVKTILGLGPNRSILGTIIRPDPVLLK
Sbjct: 361 IEDPQAPDNDIGKNSYNYFQIRSAFAMAFSILTNVKTILGLGPNRSILGTIIRPDPVLLK 420

Query: 421 RKGGRDGEVTFTSLLPGAGEPIQQQPEYGDGQEMLCNWQFGDDEPLPRGNDASRNLDTPS 480
           RKGGRDGEVTFTSLLPGAGEPIQQQPEYGDGQEMLCNWQFGDDEPLPRGNDASRNLDTPS
Sbjct: 421 RKGGRDGEVTFTSLLPGAGEPIQQQPEYGDGQEMLCNWQFGDDEPLPRGNDASRNLDTPS 480

Query: 481 SKKKRKSRCEKSRNKDKENHGSSKRSKEKSSRKKRWRENGSDGRRSPWKR 531
           SKKKRKSRCEKSRNKDKENHGSSKRSKEKSSRKKRWRENGSDGRRSPWKR
Sbjct: 481 SKKKRKSRCEKSRNKDKENHGSSKRSKEKSSRKKRWRENGSDGRRSPWKR 530

BLAST of CmaCh09G008710 vs. ExPASy TrEMBL
Match: A0A6J1F9G2 (non-canonical poly(A) RNA polymerase PAPD5 OS=Cucurbita moschata OX=3662 GN=LOC111443297 PE=4 SV=1)

HSP 1 Score: 1023.5 bits (2645), Expect = 3.2e-295
Identity = 524/530 (98.87%), Postives = 526/530 (99.25%), Query Frame = 0

Query: 1   MEEETVQQYLYDTLSPLSFSAVATTGSEEQLSPSDNLEPYAVFRNEISLSTSHSAPAETA 60
           MEEETV QYLYDTLSPLSFSAVATTGSEEQLSPSDNLEPYAVFRNEISLSTSHSAPAETA
Sbjct: 1   MEEETVPQYLYDTLSPLSFSAVATTGSEEQLSPSDNLEPYAVFRNEISLSTSHSAPAETA 60

Query: 61  ATEFFALDVAADNVEEISGFSTPLAVTSVQETEPRTPECEDQSMLESGWFRGNGGFKSPM 120
           ATEFFALDVAADNVEEISGFSTPLAVTSVQETEPRTPECEDQSMLESGWFRGNGGFKSPM
Sbjct: 61  ATEFFALDVAADNVEEISGFSTPLAVTSVQETEPRTPECEDQSMLESGWFRGNGGFKSPM 120

Query: 121 LQLHKEIVDFCEFLSPTEEERVARDSAVERVFSVVKHIWPHCKVEVFGSFQTGLYLPTSD 180
           LQLHKEIVDFCEFLSPTEEERVARDSAVERVFSVVKHIWPHCKVEVFGSFQTGLYLPTSD
Sbjct: 121 LQLHKEIVDFCEFLSPTEEERVARDSAVERVFSVVKHIWPHCKVEVFGSFQTGLYLPTSD 180

Query: 181 IDVVILDSGIPKPQLGLQALSRALSQKGIAKKIQVIGKARVPIIKFVEKQSGISFDISFD 240
           IDVVILDSGIPKPQLGLQALSRALSQKGIAKKIQVIGKARVPIIKFVEKQSGISFD+SFD
Sbjct: 181 IDVVILDSGIPKPQLGLQALSRALSQKGIAKKIQVIGKARVPIIKFVEKQSGISFDMSFD 240

Query: 241 VQNGPKAADFIKGAISKWPPLRPLCLILKVFLQQRELNEVYSGGLGSYALLTMLMAMLQS 300
           VQNGPKAADFIKGAISKWPPLRPLCLILKVFLQQRELNEVYSGGLGSYALLTMLMAMLQS
Sbjct: 241 VQNGPKAADFIKGAISKWPPLRPLCLILKVFLQQRELNEVYSGGLGSYALLTMLMAMLQS 300

Query: 301 FNVSHSSLEHNLGVLLVHFFDFYGRKLNTSDVGVSCNTGGIFFSKSNRGFMTKGRPFLLS 360
           FNVSHSSLEHNLGVLLVHFFDFYGRKLNTSDVGVSCNTGGIFFSKSNRGFMTKGRPFLLS
Sbjct: 301 FNVSHSSLEHNLGVLLVHFFDFYGRKLNTSDVGVSCNTGGIFFSKSNRGFMTKGRPFLLS 360

Query: 361 IEDPQAPDNDIGKNSYNYFQIRSAFAMAFSILTNVKTILGLGPNRSILGTIIRPDPVLLK 420
           IEDPQAPDNDIGKNSYNYFQIRSAFAMAFSILTNVKTILGLGPNRSILGTIIRPDPVLLK
Sbjct: 361 IEDPQAPDNDIGKNSYNYFQIRSAFAMAFSILTNVKTILGLGPNRSILGTIIRPDPVLLK 420

Query: 421 RKGGRDGEVTFTSLLPGAGEPIQQQPEYGDGQEMLCNWQFGDDEPLPRGNDASRNLDTPS 480
           RKGGRDGEVTFTSLLPGAGEPIQQQPEYGDGQEMLCNWQFGDDEPLPRGNDAS NLDTPS
Sbjct: 421 RKGGRDGEVTFTSLLPGAGEPIQQQPEYGDGQEMLCNWQFGDDEPLPRGNDASGNLDTPS 480

Query: 481 SKKKRKSRCEKSRNKDKENHGSSKRSKEKSSRKKRWRENGSDGRRSPWKR 531
           SKKKRKSR EKSRNK+KEN GSSKRSKEKSSRKKRWRENGSDGRRSPWKR
Sbjct: 481 SKKKRKSR-EKSRNKEKENRGSSKRSKEKSSRKKRWRENGSDGRRSPWKR 529

BLAST of CmaCh09G008710 vs. ExPASy TrEMBL
Match: A0A1S3BTA1 (non-canonical poly(A) RNA polymerase PAPD5 OS=Cucumis melo OX=3656 GN=LOC103493489 PE=4 SV=1)

HSP 1 Score: 884.4 bits (2284), Expect = 2.3e-253
Identity = 464/544 (85.29%), Postives = 488/544 (89.71%), Query Frame = 0

Query: 3   EETVQQYLYDTLSPLSFSAVATTGSEEQLSPSDNLEPYAVFRNEISLSTSHSAPAETAAT 62
           EE VQQYLYDTLSPLSFS ++TT + +QLS  D+LEPY+VFRNEISLST H APAE AAT
Sbjct: 4   EEAVQQYLYDTLSPLSFSPISTTAAGDQLSSPDDLEPYSVFRNEISLSTPHCAPAEAAAT 63

Query: 63  EFFALDVAADNVEEIS-GFSTPLAVTSVQETEPRTPEC--EDQSMLESGWFRGNGGFKSP 122
           EFFALDVA+DNVEE S GFSTPL VTS  ETEPRTPEC  EDQS LESGWFRGN GFKSP
Sbjct: 64  EFFALDVASDNVEENSGGFSTPLLVTSALETEPRTPECEGEDQSRLESGWFRGNSGFKSP 123

Query: 123 MLQLHKEIVDFCEFLSPTEEERVARDSAVERVFSVVKHIWPHCKVEVFGSFQTGLYLPTS 182
           MLQLHKEIVDFCEFLSPTEEER ARD+AVERVFSVVKHIWPHCKVEVFGSFQTGLYLPTS
Sbjct: 124 MLQLHKEIVDFCEFLSPTEEERFARDNAVERVFSVVKHIWPHCKVEVFGSFQTGLYLPTS 183

Query: 183 DIDVVILDSGIPKPQLGLQALSRALSQKGIAKKIQVIGKARVPIIKFVEKQSGISFDISF 242
           DIDVVIL SGIPKPQLGLQALSRALSQ+G+AKKIQVIGKARVPIIKF+EKQSGISFDISF
Sbjct: 184 DIDVVILGSGIPKPQLGLQALSRALSQRGMAKKIQVIGKARVPIIKFIEKQSGISFDISF 243

Query: 243 DVQNGPKAADFIKGAISKWPPLRPLCLILKVFLQQRELNEVYSGGLGSYALLTMLMAMLQ 302
           DVQNGPKAADFIKGAISKWPPLRPL LILKVFLQQRELNEVYSGGLGSYALLTMLMAMLQ
Sbjct: 244 DVQNGPKAADFIKGAISKWPPLRPLSLILKVFLQQRELNEVYSGGLGSYALLTMLMAMLQ 303

Query: 303 SFNVSHSSLEHNLGVLLVHFFDFYGRKLNTSDVGVSCNTGGIFFSKSNRGFMTKGRPFLL 362
           S NV HSSLEHNLGVLLVHFFDFYGRKLNTSDVGVSCN GGIFFSKS RGFMTKGRP LL
Sbjct: 304 SINVQHSSLEHNLGVLLVHFFDFYGRKLNTSDVGVSCNAGGIFFSKSYRGFMTKGRPCLL 363

Query: 363 SIEDPQAPDNDIGKNSYNYFQIRSAFAMAFSILTNVKTILGLGPNRSILGTIIRPDPVLL 422
           SIEDPQAPDNDIGKNS+NYFQIRSAFAMA+SILTNVKTILGLGPNRSILGTIIRPDPVLL
Sbjct: 364 SIEDPQAPDNDIGKNSFNYFQIRSAFAMAYSILTNVKTILGLGPNRSILGTIIRPDPVLL 423

Query: 423 KRKGGRDGEVTFTSLLPGAGEPIQQQPEYGDGQEMLCNWQFGDDEPLPRGNDASRNLDTP 482
           KRKGGR GEVTF SLLPGAGEP+QQ  + GD QEMLCNWQFGDDEPLPRGN+   N+ TP
Sbjct: 424 KRKGGRHGEVTFNSLLPGAGEPLQQPDQCGDDQEMLCNWQFGDDEPLPRGNNTPENVGTP 483

Query: 483 SSKKKRKSRCEKSRNKDKENHGSSKR------SKEKSSRKKRWRENGSD-------GRRS 531
           SSKKKRK+R EKSR K+KE+H S +R       KEKSS+KKR R+NG+D       GRRS
Sbjct: 484 SSKKKRKTR-EKSRKKEKESHMSKRRHEDNGSRKEKSSKKKRSRQNGNDANGLWNAGRRS 543

BLAST of CmaCh09G008710 vs. ExPASy TrEMBL
Match: A0A0A0L271 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G637740 PE=4 SV=1)

HSP 1 Score: 873.6 bits (2256), Expect = 4.1e-250
Identity = 462/543 (85.08%), Postives = 484/543 (89.13%), Query Frame = 0

Query: 3   EETVQQYLYDTLSPLSFSAVATTGSEEQL-SPSDNLEPYAVFRNEISLSTSHSAPAETAA 62
           EE VQ YLYDTLSPLSFSA+ TT + +QL SP  +LEPY+VFRNEISLST   APAETAA
Sbjct: 4   EEAVQHYLYDTLSPLSFSAITTTTTGDQLSSPDVDLEPYSVFRNEISLSTPDCAPAETAA 63

Query: 63  TEFFALDVAADNVEEISGF-STPLAVTSVQETEPRTPECEDQSMLESGWFRGNGGFKSPM 122
           TEFFALDVAAD  EE SG  S+PL VTS  ETEPRTPECEDQS LESGWFRGN G KSPM
Sbjct: 64  TEFFALDVAADKGEENSGICSSPLPVTSALETEPRTPECEDQSRLESGWFRGNSGLKSPM 123

Query: 123 LQLHKEIVDFCEFLSPTEEERVARDSAVERVFSVVKHIWPHCKVEVFGSFQTGLYLPTSD 182
           LQLHKEIVDFCEFLSPTEEERVARDSAVERVFSVVKHIWPHCKVEVFGSFQTGLYLPTSD
Sbjct: 124 LQLHKEIVDFCEFLSPTEEERVARDSAVERVFSVVKHIWPHCKVEVFGSFQTGLYLPTSD 183

Query: 183 IDVVILDSGIPKPQLGLQALSRALSQKGIAKKIQVIGKARVPIIKFVEKQSGISFDISFD 242
           IDVVIL SGIPKPQLGLQALSRALSQKGIAKKIQVIGKARVPIIKF+EKQSGISFDISFD
Sbjct: 184 IDVVILGSGIPKPQLGLQALSRALSQKGIAKKIQVIGKARVPIIKFIEKQSGISFDISFD 243

Query: 243 VQNGPKAADFIKGAISKWPPLRPLCLILKVFLQQRELNEVYSGGLGSYALLTMLMAMLQS 302
           VQNGPKAADFIKGA+SKWPPLRPLCLILKVFLQQRELNEVYSGGLGSYALLTMLMAMLQS
Sbjct: 244 VQNGPKAADFIKGAVSKWPPLRPLCLILKVFLQQRELNEVYSGGLGSYALLTMLMAMLQS 303

Query: 303 FNVSHSSLEHNLGVLLVHFFDFYGRKLNTSDVGVSCNTGGIFFSKSNRGFMTKGRPFLLS 362
            NV  SSLEHNLGVLLVHFFDFYGRKLNTSDVGVSCN GGIFFSKS RGFMTKGRP LLS
Sbjct: 304 INVPPSSLEHNLGVLLVHFFDFYGRKLNTSDVGVSCNAGGIFFSKSYRGFMTKGRPCLLS 363

Query: 363 IEDPQAPDNDIGKNSYNYFQIRSAFAMAFSILTNVKTILGLGPNRSILGTIIRPDPVLLK 422
           IEDPQAPDNDIGKNS+NYFQIRSAFAMA+SILTNVKT+LGLGPNRSILGTIIRPDPVLLK
Sbjct: 364 IEDPQAPDNDIGKNSFNYFQIRSAFAMAYSILTNVKTVLGLGPNRSILGTIIRPDPVLLK 423

Query: 423 RKGGRDGEVTFTSLLPGAGEPIQQQPEYGDGQEMLCNWQFGDDEPLPRGNDASRNLDTPS 482
           RKGGR GEVTF SLLPGAGEP+ QQPEYGD QEMLCNWQFGD+EPLPRGND   N+ TPS
Sbjct: 424 RKGGRHGEVTFNSLLPGAGEPV-QQPEYGDDQEMLCNWQFGDEEPLPRGNDTPENVGTPS 483

Query: 483 SKKKRKSRCEKSRNKDKENHGSSKR------SKEKSSRKKRWRENGSD-------GRRSP 531
           SKK+RK+R EKSR K+KE+H S +R       KE+SS+KKR R+N SD       GRRS 
Sbjct: 484 SKKQRKTR-EKSRKKEKESHSSKRRHEDNGSRKEQSSKKKRLRQNDSDANGLWNAGRRSI 543

BLAST of CmaCh09G008710 vs. ExPASy TrEMBL
Match: A0A6J1CDS2 (non-canonical poly(A) RNA polymerase PAPD5 OS=Momordica charantia OX=3673 GN=LOC111010551 PE=4 SV=1)

HSP 1 Score: 872.5 bits (2253), Expect = 9.2e-250
Identity = 458/544 (84.19%), Postives = 482/544 (88.60%), Query Frame = 0

Query: 1   MEEETVQQYLYDTLSPLSFSAVATTGSEEQLSPSDNLEPYAVFRNEISLSTSHSAPAETA 60
           MEEET+QQ+LYDTLSPLS +A  TT SE+QLSPS +LEPY+VFRN+ISLSTSH AP ETA
Sbjct: 1   MEEETIQQFLYDTLSPLSLTAATTTSSEDQLSPSGDLEPYSVFRNQISLSTSHCAPVETA 60

Query: 61  ATEFFALDVAADNVEEISGFSTPLAVTSVQETEPRTPECEDQSMLESGWFRGNGGFKSPM 120
           A EFF+LDV ADNVEEISGFSTPL   SV ETEPRTPE EDQ+ LESGWFRGN  FKSPM
Sbjct: 61  APEFFSLDVGADNVEEISGFSTPLPGASVLETEPRTPEPEDQAKLESGWFRGNSRFKSPM 120

Query: 121 LQLHKEIVDFCEFLSPTEEERVARDSAVERVFSVVKHIWPHCKVEVFGSFQTGLYLPTSD 180
           LQLHKEIVDFCEFLSPT EER  RD AVERVFSVVKHIWPHCKVEVFGSFQTGLYLPTSD
Sbjct: 121 LQLHKEIVDFCEFLSPTGEERDERDKAVERVFSVVKHIWPHCKVEVFGSFQTGLYLPTSD 180

Query: 181 IDVVILDSGIPKPQLGLQALSRALSQKGIAKKIQVIGKARVPIIKFVEKQSGISFDISFD 240
           IDVVIL SGIPKPQLGLQALSRALSQKGIAKKIQVIGKARVPIIKFVEKQSGI+FDISFD
Sbjct: 181 IDVVILGSGIPKPQLGLQALSRALSQKGIAKKIQVIGKARVPIIKFVEKQSGIAFDISFD 240

Query: 241 VQNGPKAADFIKGAISKWPPLRPLCLILKVFLQQRELNEVYSGGLGSYALLTMLMAMLQS 300
           VQNGPKAAD+IK A+ KWPPLRPLCLILKVFLQQRELNEVYSGGLGSYALLTMLMAMLQS
Sbjct: 241 VQNGPKAADYIKVAVDKWPPLRPLCLILKVFLQQRELNEVYSGGLGSYALLTMLMAMLQS 300

Query: 301 FNVSHSSLEHNLGVLLVHFFDFYGRKLNTSDVGVSCNTGGIFFSKSNRGFMTKGRPFLLS 360
            N   SSLEHNLGVLLV+FFDFYGRKLNTSDVGVSCN GGIFFSKS RGFMTK RPFLLS
Sbjct: 301 HNSCQSSLEHNLGVLLVNFFDFYGRKLNTSDVGVSCNGGGIFFSKSKRGFMTKARPFLLS 360

Query: 361 IEDPQAPDNDIGKNSYNYFQIRSAFAMAFSILTNVKTILGLGPNRSILGTIIRPDPVLLK 420
           IEDPQAPDNDIGKNS+NYFQIRSAFA+AFS LTNVKTILGLGPNRSILGTIIRPDPVLLK
Sbjct: 361 IEDPQAPDNDIGKNSFNYFQIRSAFALAFSTLTNVKTILGLGPNRSILGTIIRPDPVLLK 420

Query: 421 RKGGRDGEVTFTSLLPGAGEPIQQQPEYGDGQEMLCNWQFGDD-EPLPRGNDASRNLDTP 480
           RKGG DGEVTF SLLPGAGEPIQ Q  YG+ QEMLCNWQFGDD EPLPRGNDAS +  TP
Sbjct: 421 RKGGTDGEVTFNSLLPGAGEPIQLQ--YGNEQEMLCNWQFGDDEEPLPRGNDASEDFGTP 480

Query: 481 SSKKKRKSRC-EKSRNKDKENHGSSKR------SKEKSSRKKRWRENGSD-------GRR 530
           SS+KKRK  C EKS+ K++EN  S +R      SKEKSS+KKRWR+NGSD       GRR
Sbjct: 481 SSRKKRKVSCREKSKKKERENSSSKRRHEENGYSKEKSSKKKRWRQNGSDANGFSHSGRR 540

BLAST of CmaCh09G008710 vs. NCBI nr
Match: XP_022976478.1 (non-canonical poly(A) RNA polymerase PAPD5 [Cucurbita maxima] >XP_022976479.1 non-canonical poly(A) RNA polymerase PAPD5 [Cucurbita maxima])

HSP 1 Score: 1042.7 bits (2695), Expect = 1.1e-300
Identity = 530/530 (100.00%), Postives = 530/530 (100.00%), Query Frame = 0

Query: 1   MEEETVQQYLYDTLSPLSFSAVATTGSEEQLSPSDNLEPYAVFRNEISLSTSHSAPAETA 60
           MEEETVQQYLYDTLSPLSFSAVATTGSEEQLSPSDNLEPYAVFRNEISLSTSHSAPAETA
Sbjct: 1   MEEETVQQYLYDTLSPLSFSAVATTGSEEQLSPSDNLEPYAVFRNEISLSTSHSAPAETA 60

Query: 61  ATEFFALDVAADNVEEISGFSTPLAVTSVQETEPRTPECEDQSMLESGWFRGNGGFKSPM 120
           ATEFFALDVAADNVEEISGFSTPLAVTSVQETEPRTPECEDQSMLESGWFRGNGGFKSPM
Sbjct: 61  ATEFFALDVAADNVEEISGFSTPLAVTSVQETEPRTPECEDQSMLESGWFRGNGGFKSPM 120

Query: 121 LQLHKEIVDFCEFLSPTEEERVARDSAVERVFSVVKHIWPHCKVEVFGSFQTGLYLPTSD 180
           LQLHKEIVDFCEFLSPTEEERVARDSAVERVFSVVKHIWPHCKVEVFGSFQTGLYLPTSD
Sbjct: 121 LQLHKEIVDFCEFLSPTEEERVARDSAVERVFSVVKHIWPHCKVEVFGSFQTGLYLPTSD 180

Query: 181 IDVVILDSGIPKPQLGLQALSRALSQKGIAKKIQVIGKARVPIIKFVEKQSGISFDISFD 240
           IDVVILDSGIPKPQLGLQALSRALSQKGIAKKIQVIGKARVPIIKFVEKQSGISFDISFD
Sbjct: 181 IDVVILDSGIPKPQLGLQALSRALSQKGIAKKIQVIGKARVPIIKFVEKQSGISFDISFD 240

Query: 241 VQNGPKAADFIKGAISKWPPLRPLCLILKVFLQQRELNEVYSGGLGSYALLTMLMAMLQS 300
           VQNGPKAADFIKGAISKWPPLRPLCLILKVFLQQRELNEVYSGGLGSYALLTMLMAMLQS
Sbjct: 241 VQNGPKAADFIKGAISKWPPLRPLCLILKVFLQQRELNEVYSGGLGSYALLTMLMAMLQS 300

Query: 301 FNVSHSSLEHNLGVLLVHFFDFYGRKLNTSDVGVSCNTGGIFFSKSNRGFMTKGRPFLLS 360
           FNVSHSSLEHNLGVLLVHFFDFYGRKLNTSDVGVSCNTGGIFFSKSNRGFMTKGRPFLLS
Sbjct: 301 FNVSHSSLEHNLGVLLVHFFDFYGRKLNTSDVGVSCNTGGIFFSKSNRGFMTKGRPFLLS 360

Query: 361 IEDPQAPDNDIGKNSYNYFQIRSAFAMAFSILTNVKTILGLGPNRSILGTIIRPDPVLLK 420
           IEDPQAPDNDIGKNSYNYFQIRSAFAMAFSILTNVKTILGLGPNRSILGTIIRPDPVLLK
Sbjct: 361 IEDPQAPDNDIGKNSYNYFQIRSAFAMAFSILTNVKTILGLGPNRSILGTIIRPDPVLLK 420

Query: 421 RKGGRDGEVTFTSLLPGAGEPIQQQPEYGDGQEMLCNWQFGDDEPLPRGNDASRNLDTPS 480
           RKGGRDGEVTFTSLLPGAGEPIQQQPEYGDGQEMLCNWQFGDDEPLPRGNDASRNLDTPS
Sbjct: 421 RKGGRDGEVTFTSLLPGAGEPIQQQPEYGDGQEMLCNWQFGDDEPLPRGNDASRNLDTPS 480

Query: 481 SKKKRKSRCEKSRNKDKENHGSSKRSKEKSSRKKRWRENGSDGRRSPWKR 531
           SKKKRKSRCEKSRNKDKENHGSSKRSKEKSSRKKRWRENGSDGRRSPWKR
Sbjct: 481 SKKKRKSRCEKSRNKDKENHGSSKRSKEKSSRKKRWRENGSDGRRSPWKR 530

BLAST of CmaCh09G008710 vs. NCBI nr
Match: XP_022936827.1 (non-canonical poly(A) RNA polymerase PAPD5 [Cucurbita moschata] >XP_022936828.1 non-canonical poly(A) RNA polymerase PAPD5 [Cucurbita moschata])

HSP 1 Score: 1023.5 bits (2645), Expect = 6.7e-295
Identity = 524/530 (98.87%), Postives = 526/530 (99.25%), Query Frame = 0

Query: 1   MEEETVQQYLYDTLSPLSFSAVATTGSEEQLSPSDNLEPYAVFRNEISLSTSHSAPAETA 60
           MEEETV QYLYDTLSPLSFSAVATTGSEEQLSPSDNLEPYAVFRNEISLSTSHSAPAETA
Sbjct: 1   MEEETVPQYLYDTLSPLSFSAVATTGSEEQLSPSDNLEPYAVFRNEISLSTSHSAPAETA 60

Query: 61  ATEFFALDVAADNVEEISGFSTPLAVTSVQETEPRTPECEDQSMLESGWFRGNGGFKSPM 120
           ATEFFALDVAADNVEEISGFSTPLAVTSVQETEPRTPECEDQSMLESGWFRGNGGFKSPM
Sbjct: 61  ATEFFALDVAADNVEEISGFSTPLAVTSVQETEPRTPECEDQSMLESGWFRGNGGFKSPM 120

Query: 121 LQLHKEIVDFCEFLSPTEEERVARDSAVERVFSVVKHIWPHCKVEVFGSFQTGLYLPTSD 180
           LQLHKEIVDFCEFLSPTEEERVARDSAVERVFSVVKHIWPHCKVEVFGSFQTGLYLPTSD
Sbjct: 121 LQLHKEIVDFCEFLSPTEEERVARDSAVERVFSVVKHIWPHCKVEVFGSFQTGLYLPTSD 180

Query: 181 IDVVILDSGIPKPQLGLQALSRALSQKGIAKKIQVIGKARVPIIKFVEKQSGISFDISFD 240
           IDVVILDSGIPKPQLGLQALSRALSQKGIAKKIQVIGKARVPIIKFVEKQSGISFD+SFD
Sbjct: 181 IDVVILDSGIPKPQLGLQALSRALSQKGIAKKIQVIGKARVPIIKFVEKQSGISFDMSFD 240

Query: 241 VQNGPKAADFIKGAISKWPPLRPLCLILKVFLQQRELNEVYSGGLGSYALLTMLMAMLQS 300
           VQNGPKAADFIKGAISKWPPLRPLCLILKVFLQQRELNEVYSGGLGSYALLTMLMAMLQS
Sbjct: 241 VQNGPKAADFIKGAISKWPPLRPLCLILKVFLQQRELNEVYSGGLGSYALLTMLMAMLQS 300

Query: 301 FNVSHSSLEHNLGVLLVHFFDFYGRKLNTSDVGVSCNTGGIFFSKSNRGFMTKGRPFLLS 360
           FNVSHSSLEHNLGVLLVHFFDFYGRKLNTSDVGVSCNTGGIFFSKSNRGFMTKGRPFLLS
Sbjct: 301 FNVSHSSLEHNLGVLLVHFFDFYGRKLNTSDVGVSCNTGGIFFSKSNRGFMTKGRPFLLS 360

Query: 361 IEDPQAPDNDIGKNSYNYFQIRSAFAMAFSILTNVKTILGLGPNRSILGTIIRPDPVLLK 420
           IEDPQAPDNDIGKNSYNYFQIRSAFAMAFSILTNVKTILGLGPNRSILGTIIRPDPVLLK
Sbjct: 361 IEDPQAPDNDIGKNSYNYFQIRSAFAMAFSILTNVKTILGLGPNRSILGTIIRPDPVLLK 420

Query: 421 RKGGRDGEVTFTSLLPGAGEPIQQQPEYGDGQEMLCNWQFGDDEPLPRGNDASRNLDTPS 480
           RKGGRDGEVTFTSLLPGAGEPIQQQPEYGDGQEMLCNWQFGDDEPLPRGNDAS NLDTPS
Sbjct: 421 RKGGRDGEVTFTSLLPGAGEPIQQQPEYGDGQEMLCNWQFGDDEPLPRGNDASGNLDTPS 480

Query: 481 SKKKRKSRCEKSRNKDKENHGSSKRSKEKSSRKKRWRENGSDGRRSPWKR 531
           SKKKRKSR EKSRNK+KEN GSSKRSKEKSSRKKRWRENGSDGRRSPWKR
Sbjct: 481 SKKKRKSR-EKSRNKEKENRGSSKRSKEKSSRKKRWRENGSDGRRSPWKR 529

BLAST of CmaCh09G008710 vs. NCBI nr
Match: XP_023535086.1 (non-canonical poly(A) RNA polymerase PAPD5 [Cucurbita pepo subsp. pepo] >XP_023535087.1 non-canonical poly(A) RNA polymerase PAPD5 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1023.1 bits (2644), Expect = 8.7e-295
Identity = 524/530 (98.87%), Postives = 526/530 (99.25%), Query Frame = 0

Query: 1   MEEETVQQYLYDTLSPLSFSAVATTGSEEQLSPSDNLEPYAVFRNEISLSTSHSAPAETA 60
           MEEETV QYLYDTLSPLSFSAVATTGSEE+LSPSDNLEPYAVFRNEISLSTSHSAPAETA
Sbjct: 1   MEEETVPQYLYDTLSPLSFSAVATTGSEEKLSPSDNLEPYAVFRNEISLSTSHSAPAETA 60

Query: 61  ATEFFALDVAADNVEEISGFSTPLAVTSVQETEPRTPECEDQSMLESGWFRGNGGFKSPM 120
           ATEFFALDVAADNVEEISGFSTPLAVTSVQETEPRTPECEDQSMLESGWFRGNGGFKSPM
Sbjct: 61  ATEFFALDVAADNVEEISGFSTPLAVTSVQETEPRTPECEDQSMLESGWFRGNGGFKSPM 120

Query: 121 LQLHKEIVDFCEFLSPTEEERVARDSAVERVFSVVKHIWPHCKVEVFGSFQTGLYLPTSD 180
           LQLHKEIVDFCEFLSPTEEERVARDSAVERVFSVVKHIWPHCKVEVFGSFQTGLYLPTSD
Sbjct: 121 LQLHKEIVDFCEFLSPTEEERVARDSAVERVFSVVKHIWPHCKVEVFGSFQTGLYLPTSD 180

Query: 181 IDVVILDSGIPKPQLGLQALSRALSQKGIAKKIQVIGKARVPIIKFVEKQSGISFDISFD 240
           IDVVILDSGIPKPQLGLQALSRALSQKGIAKKIQVIGKARVPIIKFVEKQSGISFDISFD
Sbjct: 181 IDVVILDSGIPKPQLGLQALSRALSQKGIAKKIQVIGKARVPIIKFVEKQSGISFDISFD 240

Query: 241 VQNGPKAADFIKGAISKWPPLRPLCLILKVFLQQRELNEVYSGGLGSYALLTMLMAMLQS 300
           VQNGPKAADFIKGAISKWPPLRPLCLILKVFLQQRELNEVYSGGLGSYALLTMLMAMLQS
Sbjct: 241 VQNGPKAADFIKGAISKWPPLRPLCLILKVFLQQRELNEVYSGGLGSYALLTMLMAMLQS 300

Query: 301 FNVSHSSLEHNLGVLLVHFFDFYGRKLNTSDVGVSCNTGGIFFSKSNRGFMTKGRPFLLS 360
           FNVSHSSLEHNLGVLLVHFFDFYGRKLNTSDVGVSCNTGGIFFSKSNRGFMTKGRPFLLS
Sbjct: 301 FNVSHSSLEHNLGVLLVHFFDFYGRKLNTSDVGVSCNTGGIFFSKSNRGFMTKGRPFLLS 360

Query: 361 IEDPQAPDNDIGKNSYNYFQIRSAFAMAFSILTNVKTILGLGPNRSILGTIIRPDPVLLK 420
           IEDPQAPDNDIGKNSYNYFQIRSAFAMAFSILTNVKTILGLGPNRSILGTIIRPDPVLLK
Sbjct: 361 IEDPQAPDNDIGKNSYNYFQIRSAFAMAFSILTNVKTILGLGPNRSILGTIIRPDPVLLK 420

Query: 421 RKGGRDGEVTFTSLLPGAGEPIQQQPEYGDGQEMLCNWQFGDDEPLPRGNDASRNLDTPS 480
           RKGGRDGEVTFTSLLPGAGEPIQQQPEYGDGQEMLCNWQFGDDEPLPRGNDAS NLDTPS
Sbjct: 421 RKGGRDGEVTFTSLLPGAGEPIQQQPEYGDGQEMLCNWQFGDDEPLPRGNDASGNLDTPS 480

Query: 481 SKKKRKSRCEKSRNKDKENHGSSKRSKEKSSRKKRWRENGSDGRRSPWKR 531
           SKKKRKSR EKSRNK+KEN GSSKRSKEKSSRKKRWRENGSDGRRSPWKR
Sbjct: 481 SKKKRKSR-EKSRNKEKENRGSSKRSKEKSSRKKRWRENGSDGRRSPWKR 529

BLAST of CmaCh09G008710 vs. NCBI nr
Match: KAG7024837.1 (Non-canonical poly(A) RNA polymerase PAPD5 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1007.7 bits (2604), Expect = 3.8e-290
Identity = 525/563 (93.25%), Postives = 526/563 (93.43%), Query Frame = 0

Query: 1   MEEETVQQYLYDTLSPLSFSAVATTGSEEQLSPSDNLEPYAVFRNEISLSTSHSAPAETA 60
           MEEETV QYLYDTLSPLSFSAVATTGSEEQLSPSDNLEPYAVFRNEISLSTSHSAPAETA
Sbjct: 1   MEEETVPQYLYDTLSPLSFSAVATTGSEEQLSPSDNLEPYAVFRNEISLSTSHSAPAETA 60

Query: 61  ATEFFALDVAADNVEEISGFSTPLAVTSVQETEPRTPECEDQSMLESGWFRGNGGFKSPM 120
           ATEFFALDVAADNVEEISGFSTPLAVTSVQETEPRTPECEDQSMLESGWFRGNGGFKSPM
Sbjct: 61  ATEFFALDVAADNVEEISGFSTPLAVTSVQETEPRTPECEDQSMLESGWFRGNGGFKSPM 120

Query: 121 LQLHKEIVDFCEFLSPTEEERVARDSAVERVFSVVKHIWPHCKVEVFGSFQTGLYLPTSD 180
           LQLHKEIVDFCEFLSPTEEERVARDSAVERVFSVVKHIWPHCKVEVFGSFQTGLYLPTSD
Sbjct: 121 LQLHKEIVDFCEFLSPTEEERVARDSAVERVFSVVKHIWPHCKVEVFGSFQTGLYLPTSD 180

Query: 181 IDVVILDSGIPKPQLGLQALSRALSQKGIAKKIQVIGKARVPIIKFVEKQSGISFDISFD 240
           IDVVILDSGIPKPQLGLQALSRALSQKGIAKKIQVIGKARVPIIKFVEKQSGISFDISFD
Sbjct: 181 IDVVILDSGIPKPQLGLQALSRALSQKGIAKKIQVIGKARVPIIKFVEKQSGISFDISFD 240

Query: 241 VQNGPKAADFIKGAISKWPPLRPLCLILKVFLQQRELNEVYSGGLGSYALLTMLMAMLQS 300
           VQNGPKAADFIKGAISKWPPLRPLCLILKVFLQQRELNEVYSGGLGSYALLTMLMAMLQS
Sbjct: 241 VQNGPKAADFIKGAISKWPPLRPLCLILKVFLQQRELNEVYSGGLGSYALLTMLMAMLQS 300

Query: 301 FNVSHSSLEHNLGVLL---------------------------------VHFFDFYGRKL 360
           FNVSHSSLEHNLGVLL                                 VHFFDFYGRKL
Sbjct: 301 FNVSHSSLEHNLGVLLQDNLFSEIASGSCALWKLALLDYSFPLSNYSSTVHFFDFYGRKL 360

Query: 361 NTSDVGVSCNTGGIFFSKSNRGFMTKGRPFLLSIEDPQAPDNDIGKNSYNYFQIRSAFAM 420
           NTSDVGVSCNTGGIFFSKSNRGFMTKGRPFLLSIEDPQAPDNDIGKNSYNYFQIRSAFAM
Sbjct: 361 NTSDVGVSCNTGGIFFSKSNRGFMTKGRPFLLSIEDPQAPDNDIGKNSYNYFQIRSAFAM 420

Query: 421 AFSILTNVKTILGLGPNRSILGTIIRPDPVLLKRKGGRDGEVTFTSLLPGAGEPIQQQPE 480
           AFSILTNVKTILGLGPNRSILGTIIRPDPVLLKRKGGRDGEVTFTSLLPGAGEPIQQQPE
Sbjct: 421 AFSILTNVKTILGLGPNRSILGTIIRPDPVLLKRKGGRDGEVTFTSLLPGAGEPIQQQPE 480

Query: 481 YGDGQEMLCNWQFGDDEPLPRGNDASRNLDTPSSKKKRKSRCEKSRNKDKENHGSSKRSK 531
           YGDGQEMLCNWQFGDDEPLPRGNDAS NLDTPSSKKKRKSR EKSRNK+KEN GSSKRSK
Sbjct: 481 YGDGQEMLCNWQFGDDEPLPRGNDASGNLDTPSSKKKRKSR-EKSRNKEKENRGSSKRSK 540

BLAST of CmaCh09G008710 vs. NCBI nr
Match: KAG6591966.1 (Terminal nucleotidyltransferase 4B, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 977.6 bits (2526), Expect = 4.2e-281
Identity = 497/502 (99.00%), Postives = 498/502 (99.20%), Query Frame = 0

Query: 1   MEEETVQQYLYDTLSPLSFSAVATTGSEEQLSPSDNLEPYAVFRNEISLSTSHSAPAETA 60
           MEEETV QYLYDTLSPLSFSAVATTGSEEQLSPSDNLEPYAVFRNEISLSTSHSAPAETA
Sbjct: 1   MEEETVPQYLYDTLSPLSFSAVATTGSEEQLSPSDNLEPYAVFRNEISLSTSHSAPAETA 60

Query: 61  ATEFFALDVAADNVEEISGFSTPLAVTSVQETEPRTPECEDQSMLESGWFRGNGGFKSPM 120
           ATEFFALDVAADNVEEISGFSTPLAVTSVQETEPRTPECEDQSMLESGWFRGNGGFKSPM
Sbjct: 61  ATEFFALDVAADNVEEISGFSTPLAVTSVQETEPRTPECEDQSMLESGWFRGNGGFKSPM 120

Query: 121 LQLHKEIVDFCEFLSPTEEERVARDSAVERVFSVVKHIWPHCKVEVFGSFQTGLYLPTSD 180
           LQLHKEIVDFCEFLSPTEEERVARDSAVERVFSVVKHIWPHCKVEVFGSFQTGLYLPTSD
Sbjct: 121 LQLHKEIVDFCEFLSPTEEERVARDSAVERVFSVVKHIWPHCKVEVFGSFQTGLYLPTSD 180

Query: 181 IDVVILDSGIPKPQLGLQALSRALSQKGIAKKIQVIGKARVPIIKFVEKQSGISFDISFD 240
           IDVVILDSGIPKPQLGLQALSRALSQKGIAKKIQVIGKARVPIIKFVEKQSGISFDISFD
Sbjct: 181 IDVVILDSGIPKPQLGLQALSRALSQKGIAKKIQVIGKARVPIIKFVEKQSGISFDISFD 240

Query: 241 VQNGPKAADFIKGAISKWPPLRPLCLILKVFLQQRELNEVYSGGLGSYALLTMLMAMLQS 300
           VQNGPKAADFIKGAISKWPPLRPLCLILKVFLQQRELNEVYSGGLGSYALLTMLMAMLQS
Sbjct: 241 VQNGPKAADFIKGAISKWPPLRPLCLILKVFLQQRELNEVYSGGLGSYALLTMLMAMLQS 300

Query: 301 FNVSHSSLEHNLGVLLVHFFDFYGRKLNTSDVGVSCNTGGIFFSKSNRGFMTKGRPFLLS 360
           FNVSHSSLEHNLGVLLVHFFDFYGRKLNTSDVGVSCNTGGIFFSKSNRGFMTKGRPFLLS
Sbjct: 301 FNVSHSSLEHNLGVLLVHFFDFYGRKLNTSDVGVSCNTGGIFFSKSNRGFMTKGRPFLLS 360

Query: 361 IEDPQAPDNDIGKNSYNYFQIRSAFAMAFSILTNVKTILGLGPNRSILGTIIRPDPVLLK 420
           IEDPQAPDNDIGKNSYNYFQIRSAFAMAFSILTNVKTILGLGPNRSILGTIIRPDPVLLK
Sbjct: 361 IEDPQAPDNDIGKNSYNYFQIRSAFAMAFSILTNVKTILGLGPNRSILGTIIRPDPVLLK 420

Query: 421 RKGGRDGEVTFTSLLPGAGEPIQQQPEYGDGQEMLCNWQFGDDEPLPRGNDASRNLDTPS 480
           RKGGRDGEVTFTSLLPGAGEPIQQQPEYGDGQEMLCNWQFGDDEPLPRGNDAS NLDTPS
Sbjct: 421 RKGGRDGEVTFTSLLPGAGEPIQQQPEYGDGQEMLCNWQFGDDEPLPRGNDASGNLDTPS 480

Query: 481 SKKKRKSRCEKSRNKDKENHGS 503
           SKKKRKSR EKSRNK+KEN GS
Sbjct: 481 SKKKRKSR-EKSRNKEKENRGS 501

BLAST of CmaCh09G008710 vs. TAIR 10
Match: AT5G53770.1 (Nucleotidyltransferase family protein )

HSP 1 Score: 580.9 bits (1496), Expect = 1.1e-165
Identity = 309/516 (59.88%), Postives = 383/516 (74.22%), Query Frame = 0

Query: 9   YLYDTLSPLSFSAVATTGSEEQLSPS-DNLEPYAVFRNEISLSTSHSAPAETAATEFFAL 68
           ++YDTL PLSFS      S +   P+ +    Y+VFR EIS     + P E+A  +FF+L
Sbjct: 13  FVYDTLPPLSFS-----DSNQSPPPTHEESHQYSVFRKEISDFPDDTTPVESATVDFFSL 72

Query: 69  DVAADNVEEISGFSTPLAVTSVQETEPRTPECEDQSMLESGWFRGNGGFKSPMLQLHKEI 128
           DV  +  E      TP+ V S ++++ R  + +++  LES WF  N   K PMLQLHKEI
Sbjct: 73  DVEGETTENGVEPVTPVVVASKKKSKKR--KKDEEPRLESNWFSENSFSKIPMLQLHKEI 132

Query: 129 VDFCEFLSPTEEERVARDSAVERVFSVVKHIWPHCKVEVFGSFQTGLYLPTSDIDVVILD 188
           VDFC+FL PT+ E+  RD+AVE V SV+K+IWP CKVEVFGS++TGLYLPTSDIDVVIL+
Sbjct: 133 VDFCDFLLPTQAEKAERDAAVESVSSVIKYIWPSCKVEVFGSYKTGLYLPTSDIDVVILE 192

Query: 189 SGIPKPQLGLQALSRALSQKGIAKKIQVIGKARVPIIKFVEKQSGISFDISFDVQNGPKA 248
           SG+  PQLGL+ALSRALSQ+GIAK + VI KARVPIIKFVEK+S I+FD+SFD++NGPKA
Sbjct: 193 SGLTNPQLGLRALSRALSQRGIAKNLLVIAKARVPIIKFVEKKSNIAFDLSFDMENGPKA 252

Query: 249 ADFIKGAISKWPPLRPLCLILKVFLQQRELNEVYSGGLGSYALLTMLMAMLQSFNVSHSS 308
           A+FI+ A+SK PPLRPLCLILKVFLQQRELNEVYSGG+GSYALL ML+A L+      S+
Sbjct: 253 AEFIQDAVSKLPPLRPLCLILKVFLQQRELNEVYSGGIGSYALLAMLIAFLKYLKDGRSA 312

Query: 309 LEHNLGVLLVHFFDFYGRKLNTSDVGVSCNTGGIFFSKSNRGFMTKGRPFLLSIEDPQAP 368
            EHNLGVLLV FFDFYGRKLNT+DVG+SC  GG FFSK N+GF+ + RP L+SIEDPQ P
Sbjct: 313 PEHNLGVLLVKFFDFYGRKLNTADVGISCKMGGSFFSKYNKGFLNRARPSLISIEDPQTP 372

Query: 369 DNDIGKNSYNYFQIRSAFAMAFSILTNVKTILGLGPNRSILGTIIRPDPVLLKRKGGRDG 428
           +NDIGK+S+NYFQIRSAFAMA S LTN K IL LGPNRSILGTIIRPD VL +RKGG++G
Sbjct: 373 ENDIGKSSFNYFQIRSAFAMALSTLTNTKAILSLGPNRSILGTIIRPDRVLSERKGGQNG 432

Query: 429 EVTFTSLLPGAGEPIQQQPEYGDGQEMLCNWQFGDDE---PLPRGNDASRNLDTP----- 488
           +VTF SLLPGAGEP+  +        + CNW+  ++E     PRGND +  +DTP     
Sbjct: 433 DVTFNSLLPGAGEPLPLESNGKTNGGLFCNWELEEEEEEGSFPRGNDITPVVDTPGKKSK 492

Query: 489 --SSKKKRKSRCEKSRNKDKENHGSSKRSKEKSSRK 514
             S KKK+KS+  K  ++D E  G+S + K+K  R+
Sbjct: 493 ESSRKKKKKSKKNKEVDEDDEEEGTSTKKKKKKRRR 521

BLAST of CmaCh09G008710 vs. TAIR 10
Match: AT4G00060.1 (Nucleotidyltransferase family protein )

HSP 1 Score: 112.5 bits (280), Expect = 1.1e-24
Identity = 94/353 (26.63%), Postives = 153/353 (43.34%), Query Frame = 0

Query: 123  LHKEIVDFCEFLSPTEEERVAR----DSAVERVFSVVKHIWPHCKVEVFGSFQTGLYLPT 182
            L+ EI  FC+ ++    E +AR    + A++RV   ++ +WP  +  +FGS  TGL LP+
Sbjct: 1111 LNDEIDSFCKQVA---AENMARKPYINWAIKRVTRSLQVLWPRSRTNIFGSSATGLSLPS 1170

Query: 183  SDIDVV-----------ILDSGIPKPQLG-----LQALSRALSQKGIAK--KIQVIGKAR 242
            SD+D+V           I ++GI + + G     LQ  +R L+ +   K   ++ +    
Sbjct: 1171 SDVDLVVCLPPVRNLEPIKEAGILEGRNGIKETCLQHAARYLANQEWVKTDSLKTVENTA 1230

Query: 243  VPIIKFVEK--------------------------------------------------- 302
            +PII  V +                                                   
Sbjct: 1231 IPIIMLVVEVPCDLICSIQSPKDGPDCITVDQDSNGNTEMVGFEDSAAANSLPTNTGNLA 1290

Query: 303  -QSGISFDISFDV--QNGPKAADFIKGAISKWPPLRPLCLILKVFLQQRELNEVYSGGLG 362
                +  DISF      G +    +K    ++P   PL L+LK FL  R L++ YSGGL 
Sbjct: 1291 IAKSVRLDISFKTPSHTGLQTTQLVKDLTEQFPAATPLALVLKQFLADRTLDQSYSGGLS 1350

Query: 363  SYALLTMLMAMLQSFNVSHSSLEHNLGVLLVHFFDFYGRKLNTSDVGVSCNTGGIFFSKS 400
            SY L+ ++   LQ  +    S+  NLG LL+ F  F+G   +   + VS    GI+ ++ 
Sbjct: 1351 SYCLVLLITRFLQHEHHLGRSINQNLGGLLMDFLYFFGNVFDPRQMRVSVQGSGIYRNR- 1410

BLAST of CmaCh09G008710 vs. TAIR 10
Match: AT2G45620.1 (Nucleotidyltransferase family protein )

HSP 1 Score: 79.3 bits (194), Expect = 1.0e-14
Identity = 56/172 (32.56%), Postives = 87/172 (50.58%), Query Frame = 0

Query: 134 LSPTEEERVARDSAVERVFSVVKHIWPHCKVEVFGSFQTGLYLPTSDIDVVILDSG--IP 193
           L P EEE   +   +  + ++V   WPH K+ ++GS       P SDIDV +   G  I 
Sbjct: 445 LIPAEEELEKQRQLMAHLENLVAKEWPHAKLYLYGSCANSFGFPKSDIDVCLAIEGDDIN 504

Query: 194 KPQLGLQALSRALSQKGIAKKIQVIGKARVPIIKFVEKQSGISFDISFD----VQNGPKA 253
           K ++ L  L+  L    + + +Q + +ARVPI+K ++  +GIS DI  +    V N    
Sbjct: 505 KSEM-LLKLAEILESDNL-QNVQALTRARVPIVKLMDPVTGISCDICINNVLAVVNTKLL 564

Query: 254 ADFIKGAISKWPPLRPLCLILKVFLQQRELNEVYSGGLGSYALLTMLMAMLQ 300
            D+ +  +     LR L  I+K + + R +NE Y G L SYA + M +  LQ
Sbjct: 565 RDYAQIDVR----LRQLAFIVKHWAKSRRVNETYQGTLSSYAYVLMCIHFLQ 610

BLAST of CmaCh09G008710 vs. TAIR 10
Match: AT2G39740.1 (Nucleotidyltransferase family protein )

HSP 1 Score: 68.2 bits (165), Expect = 2.3e-11
Identity = 54/195 (27.69%), Postives = 92/195 (47.18%), Query Frame = 0

Query: 116 FKSPMLQLHKEIVDFCEFLSPTEEERVARDSAVERVFSVVKHI--WPHCKVEVFGSFQTG 175
           F  P LQ      +  + + PT  +R  R + ++++  V++ +       V+ FGSF + 
Sbjct: 6   FLDPTLQ------EILQVIKPTRADRDTRITVIDQLRDVLQSVECLRGATVQPFGSFVSN 65

Query: 176 LYLPTSDIDV--------VILDSGIPKPQLGLQALSRALSQKGIAKKIQVIGKARVPIIK 235
           L+    D+D+         IL +G  + Q  L  L RAL   G+  K+Q +  ARVPI+K
Sbjct: 66  LFTRWGDLDISVDLFSGSSILFTGKKQKQTLLGHLLRALRASGLWYKLQFVIHARVPILK 125

Query: 236 FVEKQSGISFDISFDVQNGPKAADFIKGAISKWPPLRPLCLILKVFLQQRELNEVYSGGL 295
            V     IS DIS D  +G   + F+          R L L++K + +   +N+  +G  
Sbjct: 126 VVSGHQRISCDISIDNLDGLLKSRFLFWISEIDGRFRDLVLLVKEWAKAHNINDSKTGTF 185

Query: 296 GSYALLTMLMAMLQS 301
            SY+L  +++   Q+
Sbjct: 186 NSYSLSLLVIFHFQT 194

BLAST of CmaCh09G008710 vs. TAIR 10
Match: AT3G45750.1 (Nucleotidyltransferase family protein )

HSP 1 Score: 60.5 bits (145), Expect = 4.8e-09
Identity = 51/199 (25.63%), Postives = 96/199 (48.24%), Query Frame = 0

Query: 120 MLQLHKEIVD-FCEFLSPTEEERVARDSAVERVFSVVKHIWPHCK-----VEVFGSFQTG 179
           +L L K + D +C F  P   +   R   V+ + ++   I+   +     +E +GSF   
Sbjct: 40  LLDLDKVLNDVYCSF-RPVSADYNTRKELVKNLNTMALDIYGKSEESSPVLEAYGSFVMD 99

Query: 180 LYLPTSDIDVVIL----DSGIPKPQLGLQALS------RALSQKGIAKKIQVIGKARVPI 239
           +Y   SD+DV I      S IP+ +  L+ L       R+L  +G  K ++ I  A+VPI
Sbjct: 100 MYSSQSDLDVSINFGNGTSEIPR-EKKLEILKRFAKKLRSLQGEGQVKNVESIFSAKVPI 159

Query: 240 IKFVEKQSGISFDISFDVQNGPKAADFIKGAISKWPPLRPLCLILKVFLQQRELNEVYSG 299
           +KF ++ +G+  D+S + ++G   +  ++         + LCL++K + +  E+N     
Sbjct: 160 VKFSDQGTGVECDLSVENKDGILNSQIVRIISQIDGRFQKLCLLVKHWAKAHEVNSALHR 219

Query: 300 GLGSYALLTMLMAMLQSFN 303
            L S ++  ++   LQ+ N
Sbjct: 220 TLNSVSITLLVALHLQTQN 236

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8NDF85.9e-6042.68Terminal nucleotidyltransferase 4B OS=Homo sapiens OX=9606 GN=TENT4B PE=1 SV=2[more]
Q68ED35.9e-6042.68Terminal nucleotidyltransferase 4B OS=Mus musculus OX=10090 GN=Tent4b PE=1 SV=2[more]
Q5XG877.7e-6043.14Terminal nucleotidyltransferase 4A OS=Homo sapiens OX=9606 GN=TENT4A PE=1 SV=3[more]
Q7KVS92.5e-5841.08Non-canonical poly(A) RNA polymerase protein Trf4-1 OS=Drosophila melanogaster O... [more]
Q6PB751.7e-5442.21Terminal nucleotidyltransferase 4A OS=Mus musculus OX=10090 GN=Tent4a PE=2 SV=2[more]
Match NameE-valueIdentityDescription
A0A6J1IMA15.1e-301100.00non-canonical poly(A) RNA polymerase PAPD5 OS=Cucurbita maxima OX=3661 GN=LOC111... [more]
A0A6J1F9G23.2e-29598.87non-canonical poly(A) RNA polymerase PAPD5 OS=Cucurbita moschata OX=3662 GN=LOC1... [more]
A0A1S3BTA12.3e-25385.29non-canonical poly(A) RNA polymerase PAPD5 OS=Cucumis melo OX=3656 GN=LOC1034934... [more]
A0A0A0L2714.1e-25085.08Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G637740 PE=4 SV=1[more]
A0A6J1CDS29.2e-25084.19non-canonical poly(A) RNA polymerase PAPD5 OS=Momordica charantia OX=3673 GN=LOC... [more]
Match NameE-valueIdentityDescription
XP_022976478.11.1e-300100.00non-canonical poly(A) RNA polymerase PAPD5 [Cucurbita maxima] >XP_022976479.1 no... [more]
XP_022936827.16.7e-29598.87non-canonical poly(A) RNA polymerase PAPD5 [Cucurbita moschata] >XP_022936828.1 ... [more]
XP_023535086.18.7e-29598.87non-canonical poly(A) RNA polymerase PAPD5 [Cucurbita pepo subsp. pepo] >XP_0235... [more]
KAG7024837.13.8e-29093.25Non-canonical poly(A) RNA polymerase PAPD5 [Cucurbita argyrosperma subsp. argyro... [more]
KAG6591966.14.2e-28199.00Terminal nucleotidyltransferase 4B, partial [Cucurbita argyrosperma subsp. soror... [more]
Match NameE-valueIdentityDescription
AT5G53770.11.1e-16559.88Nucleotidyltransferase family protein [more]
AT4G00060.11.1e-2426.63Nucleotidyltransferase family protein [more]
AT2G45620.11.0e-1432.56Nucleotidyltransferase family protein [more]
AT2G39740.12.3e-1127.69Nucleotidyltransferase family protein [more]
AT3G45750.14.8e-0925.63Nucleotidyltransferase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D1.10.1410.10coord: 120..394
e-value: 3.7E-98
score: 330.4
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 491..505
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 457..530
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 513..530
NoneNo IPR availablePANTHERPTHR23092POLY(A) RNA POLYMERASEcoord: 94..513
NoneNo IPR availablePANTHERPTHR23092:SF15INACTIVE NON-CANONICAL POLY(A) RNA POLYMERASE PROTEIN TRF4-2-RELATEDcoord: 94..513
NoneNo IPR availableCDDcd05402NT_PAP_TUTasecoord: 143..252
e-value: 1.05675E-36
score: 129.985
NoneNo IPR availableSUPERFAMILY81631PAP/OAS1 substrate-binding domaincoord: 259..397
IPR043519Nucleotidyltransferase superfamilyGENE3D3.30.460.10Beta Polymerase, domain 2coord: 136..244
e-value: 3.7E-98
score: 330.4
IPR043519Nucleotidyltransferase superfamilySUPERFAMILY81301Nucleotidyltransferasecoord: 120..271
IPR002934Polymerase, nucleotidyl transferase domainPFAMPF01909NTP_transf_2coord: 150..239
e-value: 3.0E-10
score: 40.2
IPR002058PAP/25A-associatedPFAMPF03828PAP_assoccoord: 311..369
e-value: 2.4E-14
score: 53.3

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh09G008710.1CmaCh09G008710.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0031123 RNA 3'-end processing
cellular_component GO:0005730 nucleolus
cellular_component GO:0031499 TRAMP complex
molecular_function GO:0004652 polynucleotide adenylyltransferase activity
molecular_function GO:0016779 nucleotidyltransferase activity