CmaCh09G008300 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh09G008300
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Descriptionsodium/calcium exchanger NCL-like
LocationCma_Chr09: 4072865 .. 4080704 (-)
RNA-Seq ExpressionCmaCh09G008300
SyntenyCmaCh09G008300
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAGTCATGGAGGAAGCAGTCTTCCAAAGTGTGTGGTGATAATTCAAAAGAGGAATTGCGAAGCATTTGGCGGCACACGCTCTAGTTTGTATCCCTCCCAGAAAGTGATGGTAAGCTTTCACGAAATTCCTTACCCTTCTTTTACTTTGAATTGGTCTTCCAAATTTCATTAGACCAATAGTTGATTTTCATATAATTTTTTTTTAAAAAAAAATCAAAATTATTTTTTATTTTTCTATAATATTTGATAGGGATCAAAATTTAAAAAAAAAAAATTGTTTAACATATTTATTAGTTTCTATCTATAATATAATATTTTAATCAGACATTTAGTCTTATAGACTAATAAATACAACATTATTCCAATCAATTAAGACAAAAAGTTCTTGTCTTGTTTGTCTTTGTCGGAACACCGTTTTGTTGTCATACAAATTACAAGACTCGTTCATAATCGTGACTATGGCAGATTTGCATCTATTTTGAGTAACACTTTACGACTTAGTCTTGTATTTGTACCAAATTAGGGATGTATATGAGTTCGAGAAATTGACCCGATCCAACCCGAATTATAAGGGTTGGATTGGATTTTTTAGGACCCGATCTAATTCGGGTTGGGTATAGATTCAGTGTGTAAAAAATTGGGTCAACCCAACCCGACCCAATTATAATATAGAATGAAAGAACTAATTATACCAAAATCTTTACTAATCAACGGAAGAGGGGGAGAACCGACATCAAAGCCCAGATTGGATTAGTGGGCCTAAAATTGCAGGCCCATTAATGGGTATAAATTCAGTGCGCGGGTCAACCCAACCGAACCCAACCCAAAATATAAAGGTTGGATTGGATTTAAAAGGATTTTCGAATTTTGGTTGGACTTGAATTCAAATTATTGGGTCAACTCGATCTAACTCGAAAATCTTTTAAAAAAAAAATAATAATAATAAAATAGGTTCAAACCCACATAGCCCAAACTCGTGAATCATCGTATGCATCTACCCTTGCCCCTAATGTGTCTGCACTCTGCAGACTACCTCTTTGACTCTCTTTGCTCTGTCTTGTAGTCTCACGCAATGCTCGTCTTTGACCTAAAGGGTACCTAGGTAGGTGAAGTGAAGTCTTGACTCTTGTACTTACAGTCTCACACACTCGCACCACAATGTTTTTCTTCATCGTTCGTCGTTCAACTTCTCTCAGCTCTCTATGTGTGTAGTCATTCGACTTCTCAATTCTCATCTCACTCTTGTCTCGTTTATGTGTGTCATCGAATGTCAACGTCACTCGCCACTTTATTTCTCTATTGGACTCTCTTCTTAGTTCTCATTTCTTCACTCAACCTCATTTTCATGTTTCTCGTCAAACATCGTCACCAACTATTTCTCCATTTGGTGCTCTGTTCCACTTTTGTTGGATTTTTCCGCTCTATGTTTGTCATCAAACTTTTCCACTCAACCCTACATTATTTCACACCTCGAACCCAACCCAAAAATAGAGAGCTCATAATATGAACTAATTTTATTTAAATTGAAATAAAAAAATCCCAACTAAATCCATATTATTATAATATTTCCAACGACTTTAGTGACACGTTGGTATTGATGTCCATAAATGAGATGGCAACCACCTTCAAACAATAATGAACTCAAATAATAATAATAATAATAATAATAATAACAATAATAATAATAATAATAATGTAAATGCCACCGAATCTATATTTATTTATTTTCAAATATTCATTTATATTTACATCTCAATAACTTCAAAGTTTATTTAGTCGGGATAAAATAATAAAAAAAAATTAAAATAAATAATACAAAAATAATTAGAACAAATAAATACATGCATACATCTTAGAAAATAATATATTCATATTTATTTAATAAGAATATACAAAAACAACTAAATTCATAAAAATTTCATTTATTTAAATTAAACTCTTGACGTGAATCTAATAAAATTAATTAAATTTATTAGATAATAGTCGAATAAACTATTTTAAAAAATAATTTGAAATTCTTTTATTAATAACCTAAAAAAAGATTCCTTTTCAATTTTTTAAATAAAAAAAAAGATTTTATTTTTGGATAAATCTCTAACCTTTCAAGCCTTGTGTTTCCATTGCCGACATCCTCCAATAAGACTTGTCCCTATAGCTTGCTGCCACGTCAGAGGTTGAGTCATTATCAATTATAAAGACAAAATCCTAAATAAAAGCTCTCGTGTCCACTTGTATCTGACGTGTCACCACCCATAAGCATAAATTAAGAGTCGTATAATCCCTCAATTTGCAATTTAATTTCCACGTCGACAAAAACTTTTTTTCATGATAAAGCAATGTGAACCGATTTCAATGTATTTAATATGTTGACTTATTTTATTCATGACGTGGGAATAACCCCTTTTAAGGATCCCTTCAAATTATTAATATTATTTTCTATCCAGATTTTCTTTAAATATCTTATCATTTGTATTGATTTTCGATTAATTAATTTAATTATTTTTTTTAACAAATTTCAAACACTCCCTTTCAAATAAATAAATAAATATATAATTTTCAAATCGTTTGAAATTGTCTAAAGTGATAAATAATTATTTAGAATATTTAACGTTCATAAATAAAATATAAATTATTTTTTTAAAAAAAATATATAATTTTATTTTTCCGTCTCCTTTAAAGATCGAGCTAGTGCAAAAACTAGTGACGATGTTGTAAAGAGATGCTTGGTTCGCGCTTTTTTTTTTTTCTATTATTATTATTCCATCTACTTTTTTTTTAATCTTTATTTTTATTTTATTTTCTTTTTGTTTTTTTAATATGGTTTTTTTTTCAATTATTTGTTATATATATATATTTTTTCAAATAATTTATAATTGAATTGAGAGAATTAAGAGGATAAATGATAATAAATTAAAATAAATATATTTAATATTGAAACAGTGAGATAAATTAATTATATTCAAAATTTTAAAATACAACATGTATAATTAATTATTTAGTCCATTATTATACTAATTATTTAAAGATAGTTATTAAAATCAATGGTTGAATAAATTAAAAAACTTAAATAGTTATTCTAAATTACAATAATTGAAATTAATATATATTTATTGTTAACATTTATAGGAACTTTAGTGTCACATCGTGTCCTTTTCTATATAACGGTTTCTTCTATGTAATAAAAAGCATATAATATAAGAATGGAGATTTTTTCAAACCCTTTTTATTATTATTTTTCATTTAAATATTTCCTAACTTTTAGTTATTTATTTTGTTTTATTTTGCTAAATTATTTTGCGAAATTCCACTTTTGTCCTTGGTCCTTATCAGTCTTCAATGCACCCCTTACAAAACTATAGCAATAAACGACGCCAAATATGGAAATATCTTATCTTATATTATTCAAAAATTTGTAACGCCTCATGTTCTAGATTCAAAATCTAGATTCGACATGATCATGTTATTTACTTTTAAGAGTGAAAACTATCTTCGTAGACTAACACAAGTTGTTTCAAACATGTGACGATTATTATTTATTGGTCGAATTTTTTGTTTCTTATAACAAAAAGAAAAACAGATAATTTTGAGTGTTTATAATTAAAAGTCTTTCATCTTCCTTGTAGAAAGGATCGGCTTCTGAACGTTGTAAGACAGAGACGCGGTTGCAACGCTGAGCCCAACAATTTTGAGTCTCAATATCTCTCTGTCCCAACCAATCCTTCTTTTTAAGCCACACCCACCAATTTCTTCCCCACACCCACTTCATCTCTCTCGCTGGAGTTCCGGATTTTGCCTCAAAATCATGTCCAGAACTCCTCTGCATCTCGTCTTCCTCCTCTGTTTCCTCATCCTTTGTGTCCAATCCCACAGCTCTCGATTCGCTTCCATTTCTTCCTCCTCCGATCTGGTTTCCGATGGCGTTAATGAATTGCAGGAACCCTCTTATCTGCGACTCAATACGCTTTCTTCGAACTCTGCACCGGAGGAATCTTGCGAGCAGTCTTATGGGTTTCTCCCCTGTACCACCTCTGTTTTGGGGAATATGTTTTTGATTATCGTTTATGGGTATTTGATGTTCTTGGCTGCTAAATACTTGTCTGCTGGAAGCGAGCTCTTGTTGGAGATTTTGGGTCCTGGAATCGTTGGGGGATTGTTCCTTCCCGCGCTTGGTGCTCTTCCTGATGCTATGCTGATTCTCGGTATTTGTTTCTTCTTAAACTTTTTGTTTTGCATTTTGATGGGTTTCGACCTATAATCGAATGTGATGCGTATTCAAATGGATTGGCTCATACGATTTGTCGAGAACACGATCGTTTGATTAGTGAAATTGATGTGTTGTTCTGATTTTACGATCCAGACAAAGACGATATGGATTAGTAAATCGGGTAGGGTTTTGATAGCAAAATGTTTGGAGTGCAAAATAAACGATAGATCTATATTTTACTTTCGTTTTCCATTCGTGAAGTGTTTGATTGCAGAAATTGAATCGGCTATTTCTTAAACGCAGTGGAAATAGAATTAGATTCTAACATAAATAGTGGGTTCTCTTTTGTTTTTTCTTGGCTACTTTTTCAAAAGAAGTTTCGGGAATTTACCCCAAAAAGATTCATAAATGTTTGAGTGTTCGTTGTTTGAAGTTCTATTTGCTTCTACACTTTTACTTTATTGTGTTTGGGTTTGCTTACTTTTGTGGCTATTCATTGATATCAACTTCATCGTACCTCAATTGTTGCTGCTTTGATATAAACCTTGTCGGTTAGACCTCAACATCAAGAACAGGAACGACTCTCGTTCCTTTGTCTTGGATTGACACCTTTTTGAAAGCTTAGCCATCAGCTCCCGAACTGGGCACAATTCATTTCAAAATTTCAGTATTGCCACCTCCCCTTGTTTTCAATGTGGATCATCCTCTAATATCTGTTGTCTTTTTGTTTGTTGAATCGTTTATACTTTATATAGAGACTTTCACCAATTGATTCGAATTCGAAGGATGATAAAACAAGCATCAAGTTTCTATGTGTGAATTGGTCATTCTCATTAGAATGATTATTATTTTAGTTTCTCCTATTTCTTTATCTCTGTTCATGGTTTCTGATAACCCTCTAATCGTCTCCTTTCTATAGTTTCTGGTCTTGCAGGAAGTGCTGAAGTTGCACAAAGCCAGGTCTCCGTTGGAATGGGCCTACTAGCTGGCTCAACTGTTATGCTTCTTACACTGATCTGGGGTACATGTGTGATCGTTGGGAAGTGTGACCTTGAGGGTTCGGTTGCCATTGACTCACAGGATACAAAAGGGTTTAGCTTGACTGGTATGTCATTTTCTCTTAGTTGTCTTCACCTTTTGTATATGTAACAGCTCAAGCTCACCGCTAGTAGGTATTGTCCTCTTTGGGTTTTCCAATTTTTAAAACGCGTCTGTTAGGGAGAGGTTTCCACACCCTTATAAATAATGTTTCGTTCTCTTCCCCAACCGATGTGGGATCTCACATTCCACCCTCCTTCGGGGCCCAGCATTCTCGTTGGCACTCGTTCCCTTCTCCAATCGATGTGGGACCCCCCAATCCACCTACTTCAGGATCCATCGTCCTTTCTGGCACACCACCTCATGTCCACCCCCTTTCGGGGCTTAGCCTCCTCGCTGACACATCGCCCGGTGTCTGACTCTGATATCATTTGTAATAGCCCAAGCCTACCGCTAGCCGATATTGTCCTTGGGCTTCCCCTCAAGGTTTTTAAAACGCGTCTGCTAGGGAGAGGTTTTCACACCCTTATAAAGAATGCTTCGTTCTCCTCCCCAACCGATGTGGGATCTCACAGTAAATACCTTCGCCAAATTCCAAGATGTCTGTTTATGTTCGTATATGCCTCTGTTTTTAGAGGGAGAGTTGGGAGGGAATCTTATTCCATCAGTTGCCTAGTAAAAGTTCTGTTCTTCTTATACATATAGTTGATGCACAAATTTACATTGGAATTTCTTCTTTGATAGAATCTGGAGTCAGCACTGATATTTGGACGAGCTATGCTGCCAGGATTATGGTTATATCTGTTGTTCCATTTTTAATAGTTCAACTTCCACAAATGCTGAATTCAACATCAGGAAGGCACTTGGCAGTGTTGATTGCTCTTATAATCTCCATTTCAATGTTCGTTATCTATTGCCTTTACCAGGTGCTTTTTCTAACTCTTACCTCTTTTTTCCCTTTAAGATGGGGCCTGCTGGACTTACTGATAACTTCTGAATTGCTAAGTACTTAACACCTTCGAGGAAGGATTCTCTATATCAAAACCGAAATGCATAACTCTAACTCGAACAGGCAGCTATTCTGATCTTCCCAAGTCGAAAAGCTATAGCCGGTTAAGAGTACCTTTTGAAATGATTTCGAGTTTAACCATTTCAAACTTGCTCCTTTGTGCAGGTCTTCCAGCCATGGATCCAAAGGAGGAAACTTGCCTTTGTGAAGCACAAGCATGTTATATTTGGACTTTTAAGACATTTAAAACAGCAAGCACTTGGAAGACTCCTGACAGAAAATGGGGAGCCAGATAAAGAGATAATAGACAAGTTCGTTGGAACCACAAGTTCAATTCTTCATGTTTATCGTTGCTTTACTAGTTCATCACATTCTGATACTTGCTCAATAAACACAATGGCAGGCTATTTTCAAGAATTGATGAGAACAAGGATGGACGTCTTTCAGCTTCTGAACTGAGAGCGCTTATAGTCGGTATCCAGTTCGAGGAGCTCGACTTAGATCACGAAGATGCTGTTGAAAAAATACTGAATGATTTCGATACTTCTCGTGATTCCCATGTCGATGCTACCGAGTTTGGCAATGGAATCATTAAGTGGCTTTCTCAAGTACAAGGTTCAAGAACTGGCCGAGCTGATGATGGTCCTCACACAATGAAGTTCCTGCATAACTTTCATCAAGTACGTCGTAAACCCGTTCGTTGCAGTTTTCCTTTTTAACTTTTGAGGTTGCTTTCTGAACCACGACGTAATGTATCTACAGGAAACAAAGCGAGAGCACGATTTACTGGATGTCGGGGAACAAAGCGACGAGGTCGTAGAGGATGTTGAGGGGGGCAAAGGGGTCTTGATCAAAGCTATACTCTTCTTGTTACTTGGAACTGCCATTGCTGCTGCATTTGCCGACCCGTTGGTCGACGTCGTACATAACTTCTCTAATGCAACTAACATCCCAGCGTTCTTTATTTCGTTTATCGCTCTGCCATTAGCAACCAATTCCAGTGAAGCAGTCTCTGCTATAATCTTCGCAAGCCGGGACAAGCGAAAAACTGCCTCTTTAACATTTTCTGAGGTTCGTTCTCTTTTACACACTAACATTTCATTTCTTTGCTCGTACTTGGAAGAATGGTTGAACTCTAAGCTTTTGATGGTGCAGCTATATGGAGCAGTGACAATGAACAATGTTCTCTGCCTATCAGTCTTCTTGGCTCTTGTTTATATGAGAGGATTGGTTTGGAACTTTTCGTCGGAAGTGCTGGTCATTCTGGTCGTTACGGTTATAATGGGACTTCTCGGTAGCTTCCGAACCGCATTCCCGCTCTGGACAGCTCTGGTGGCTCTCCTTCTCTACCCCCTCTCTCTGGTGCTGGTCTATGTTCTTGATTATGTATTTGGTTGGTCATAGATCAGGTGGTGTTTTTGAGTTATATATGCAGGGGGTTTGTTCATTTAAGTTTCCTGATGTTTTTGCACATACTCTCCATGCTGAGGCAATGTAATGTAATACAACTACTGTTCTGCTCTCTCTTTTATTTGCTTCTGATTACAGGAAATAAATAATAGAAAGAAATTCAAATATTTATGCAAG

mRNA sequence

ATGAGTCATGGAGGAAGCAGTCTTCCAAAGTGTGTGGTGATAATTCAAAAGAGGAATTGCGAAGCATTTGGCGGCACACGCTCTAGTTTGTATCCCTCCCAGAAAGTGATGCCACACCCACCAATTTCTTCCCCACACCCACTTCATCTCTCTCGCTGGAGTTCCGGATTTTGCCTCAAAATCATGTCCAGAACTCCTCTGCATCTCGTCTTCCTCCTCTGTTTCCTCATCCTTTGTGTCCAATCCCACAGCTCTCGATTCGCTTCCATTTCTTCCTCCTCCGATCTGGTTTCCGATGGCGTTAATGAATTGCAGGAACCCTCTTATCTGCGACTCAATACGCTTTCTTCGAACTCTGCACCGGAGGAATCTTGCGAGCAGTCTTATGGGTTTCTCCCCTGTACCACCTCTGTTTTGGGGAATATGTTTTTGATTATCGTTTATGGGTATTTGATGTTCTTGGCTGCTAAATACTTGTCTGCTGGAAGCGAGCTCTTGTTGGAGATTTTGGGTCCTGGAATCGTTGGGGGATTGTTCCTTCCCGCGCTTGGTGCTCTTCCTGATGCTATGCTGATTCTCGTTTCTGGTCTTGCAGGAAGTGCTGAAGTTGCACAAAGCCAGGTCTCCGTTGGAATGGGCCTACTAGCTGGCTCAACTGTTATGCTTCTTACACTGATCTGGGGTACATGTGTGATCGTTGGGAAGTGTGACCTTGAGGGTTCGGTTGCCATTGACTCACAGGATACAAAAGGGTTTAGCTTGACTGAATCTGGAGTCAGCACTGATATTTGGACGAGCTATGCTGCCAGGATTATGGTTATATCTGTTGTTCCATTTTTAATAGTTCAACTTCCACAAATGCTGAATTCAACATCAGGAAGGCACTTGGCAGTGTTGATTGCTCTTATAATCTCCATTTCAATGTTCGTTATCTATTGCCTTTACCAGGTCTTCCAGCCATGGATCCAAAGGAGGAAACTTGCCTTTGTGAAGCACAAGCATGTTATATTTGGACTTTTAAGACATTTAAAACAGCAAGCACTTGGAAGACTCCTGACAGAAAATGGGGAGCCAGATAAAGAGATAATAGACAAGCTATTTTCAAGAATTGATGAGAACAAGGATGGACGTCTTTCAGCTTCTGAACTGAGAGCGCTTATAGTCGGTATCCAGTTCGAGGAGCTCGACTTAGATCACGAAGATGCTGTTGAAAAAATACTGAATGATTTCGATACTTCTCGTGATTCCCATGTCGATGCTACCGAGTTTGGCAATGGAATCATTAAGTGGCTTTCTCAAGTACAAGGTTCAAGAACTGGCCGAGCTGATGATGGTCCTCACACAATGAAGTTCCTGCATAACTTTCATCAAGAAACAAAGCGAGAGCACGATTTACTGGATGTCGGGGAACAAAGCGACGAGGTCGTAGAGGATGTTGAGGGGGGCAAAGGGGTCTTGATCAAAGCTATACTCTTCTTGTTACTTGGAACTGCCATTGCTGCTGCATTTGCCGACCCGTTGGTCGACGTCGTACATAACTTCTCTAATGCAACTAACATCCCAGCGTTCTTTATTTCGTTTATCGCTCTGCCATTAGCAACCAATTCCAGTGAAGCAGTCTCTGCTATAATCTTCGCAAGCCGGGACAAGCGAAAAACTGCCTCTTTAACATTTTCTGAGCTATATGGAGCAGTGACAATGAACAATGTTCTCTGCCTATCAGTCTTCTTGGCTCTTGTTTATATGAGAGGATTGGTTTGGAACTTTTCGTCGGAAGTGCTGGTCATTCTGGTCGTTACGGTTATAATGGGACTTCTCGGTAGCTTCCGAACCGCATTCCCGCTCTGGACAGCTCTGGTGGCTCTCCTTCTCTACCCCCTCTCTCTGGTGCTGGTCTATGTTCTTGATTATGTATTTGGTTGGTCATAGATCAGGTGGTGTTTTTGAGTTATATATGCAGGGGGTTTGTTCATTTAAGTTTCCTGATGTTTTTGCACATACTCTCCATGCTGAGGCAATGTAATGTAATACAACTACTGTTCTGCTCTCTCTTTTATTTGCTTCTGATTACAGGAAATAAATAATAGAAAGAAATTCAAATATTTATGCAAG

Coding sequence (CDS)

ATGAGTCATGGAGGAAGCAGTCTTCCAAAGTGTGTGGTGATAATTCAAAAGAGGAATTGCGAAGCATTTGGCGGCACACGCTCTAGTTTGTATCCCTCCCAGAAAGTGATGCCACACCCACCAATTTCTTCCCCACACCCACTTCATCTCTCTCGCTGGAGTTCCGGATTTTGCCTCAAAATCATGTCCAGAACTCCTCTGCATCTCGTCTTCCTCCTCTGTTTCCTCATCCTTTGTGTCCAATCCCACAGCTCTCGATTCGCTTCCATTTCTTCCTCCTCCGATCTGGTTTCCGATGGCGTTAATGAATTGCAGGAACCCTCTTATCTGCGACTCAATACGCTTTCTTCGAACTCTGCACCGGAGGAATCTTGCGAGCAGTCTTATGGGTTTCTCCCCTGTACCACCTCTGTTTTGGGGAATATGTTTTTGATTATCGTTTATGGGTATTTGATGTTCTTGGCTGCTAAATACTTGTCTGCTGGAAGCGAGCTCTTGTTGGAGATTTTGGGTCCTGGAATCGTTGGGGGATTGTTCCTTCCCGCGCTTGGTGCTCTTCCTGATGCTATGCTGATTCTCGTTTCTGGTCTTGCAGGAAGTGCTGAAGTTGCACAAAGCCAGGTCTCCGTTGGAATGGGCCTACTAGCTGGCTCAACTGTTATGCTTCTTACACTGATCTGGGGTACATGTGTGATCGTTGGGAAGTGTGACCTTGAGGGTTCGGTTGCCATTGACTCACAGGATACAAAAGGGTTTAGCTTGACTGAATCTGGAGTCAGCACTGATATTTGGACGAGCTATGCTGCCAGGATTATGGTTATATCTGTTGTTCCATTTTTAATAGTTCAACTTCCACAAATGCTGAATTCAACATCAGGAAGGCACTTGGCAGTGTTGATTGCTCTTATAATCTCCATTTCAATGTTCGTTATCTATTGCCTTTACCAGGTCTTCCAGCCATGGATCCAAAGGAGGAAACTTGCCTTTGTGAAGCACAAGCATGTTATATTTGGACTTTTAAGACATTTAAAACAGCAAGCACTTGGAAGACTCCTGACAGAAAATGGGGAGCCAGATAAAGAGATAATAGACAAGCTATTTTCAAGAATTGATGAGAACAAGGATGGACGTCTTTCAGCTTCTGAACTGAGAGCGCTTATAGTCGGTATCCAGTTCGAGGAGCTCGACTTAGATCACGAAGATGCTGTTGAAAAAATACTGAATGATTTCGATACTTCTCGTGATTCCCATGTCGATGCTACCGAGTTTGGCAATGGAATCATTAAGTGGCTTTCTCAAGTACAAGGTTCAAGAACTGGCCGAGCTGATGATGGTCCTCACACAATGAAGTTCCTGCATAACTTTCATCAAGAAACAAAGCGAGAGCACGATTTACTGGATGTCGGGGAACAAAGCGACGAGGTCGTAGAGGATGTTGAGGGGGGCAAAGGGGTCTTGATCAAAGCTATACTCTTCTTGTTACTTGGAACTGCCATTGCTGCTGCATTTGCCGACCCGTTGGTCGACGTCGTACATAACTTCTCTAATGCAACTAACATCCCAGCGTTCTTTATTTCGTTTATCGCTCTGCCATTAGCAACCAATTCCAGTGAAGCAGTCTCTGCTATAATCTTCGCAAGCCGGGACAAGCGAAAAACTGCCTCTTTAACATTTTCTGAGCTATATGGAGCAGTGACAATGAACAATGTTCTCTGCCTATCAGTCTTCTTGGCTCTTGTTTATATGAGAGGATTGGTTTGGAACTTTTCGTCGGAAGTGCTGGTCATTCTGGTCGTTACGGTTATAATGGGACTTCTCGGTAGCTTCCGAACCGCATTCCCGCTCTGGACAGCTCTGGTGGCTCTCCTTCTCTACCCCCTCTCTCTGGTGCTGGTCTATGTTCTTGATTATGTATTTGGTTGGTCATAG

Protein sequence

MSHGGSSLPKCVVIIQKRNCEAFGGTRSSLYPSQKVMPHPPISSPHPLHLSRWSSGFCLKIMSRTPLHLVFLLCFLILCVQSHSSRFASISSSSDLVSDGVNELQEPSYLRLNTLSSNSAPEESCEQSYGFLPCTTSVLGNMFLIIVYGYLMFLAAKYLSAGSELLLEILGPGIVGGLFLPALGALPDAMLILVSGLAGSAEVAQSQVSVGMGLLAGSTVMLLTLIWGTCVIVGKCDLEGSVAIDSQDTKGFSLTESGVSTDIWTSYAARIMVISVVPFLIVQLPQMLNSTSGRHLAVLIALIISISMFVIYCLYQVFQPWIQRRKLAFVKHKHVIFGLLRHLKQQALGRLLTENGEPDKEIIDKLFSRIDENKDGRLSASELRALIVGIQFEELDLDHEDAVEKILNDFDTSRDSHVDATEFGNGIIKWLSQVQGSRTGRADDGPHTMKFLHNFHQETKREHDLLDVGEQSDEVVEDVEGGKGVLIKAILFLLLGTAIAAAFADPLVDVVHNFSNATNIPAFFISFIALPLATNSSEAVSAIIFASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVYMRGLVWNFSSEVLVILVVTVIMGLLGSFRTAFPLWTALVALLLYPLSLVLVYVLDYVFGWS
Homology
BLAST of CmaCh09G008300 vs. ExPASy Swiss-Prot
Match: Q8L636 (Sodium/calcium exchanger NCL OS=Arabidopsis thaliana OX=3702 GN=NCL PE=2 SV=1)

HSP 1 Score: 712.6 bits (1838), Expect = 4.0e-204
Identity = 386/577 (66.90%), Postives = 453/577 (78.51%), Query Frame = 0

Query: 73  LCFLILCVQSHSSRFASIS-SSSDLVSDGV----NELQEPSYLRLNTLSSNSAPEESCEQ 132
           L FL+    S  +RF S++ SS+ L+SDG+    N     S ++    +     EE+CEQ
Sbjct: 9   LLFLLFFTSSAYARFVSLNPSSTSLISDGIDGGSNLAGSGSVIKSVVSAPAEEKEEACEQ 68

Query: 133 SYGFLPCTTSVLGNMFLIIVYGYLMFLAAKYLSAGSELLLEILGPGIVGGLFLPALGALP 192
           +YGF+PCT + LGN+FLI+VYG+LMF AA YLSAGSELLLEILGPGIVGGLFLP LGALP
Sbjct: 69  TYGFMPCTKTALGNVFLILVYGFLMFTAATYLSAGSELLLEILGPGIVGGLFLPMLGALP 128

Query: 193 DAMLILVSGLAGSAEVAQSQVSVGMGLLAGSTVMLLTLIWGTCVIVGKCDLEGSVAIDSQ 252
           DAMLI+VSGL+G A  AQSQVSVGMGLLAGSTVMLLT+IWGTC +VGKCDL  S+A+++Q
Sbjct: 129 DAMLIMVSGLSGDAATAQSQVSVGMGLLAGSTVMLLTVIWGTCTVVGKCDLRDSIAVNNQ 188

Query: 253 DTKGFSLTESGVSTDIWTSYAARIMVISVVPFLIVQLPQMLNSTSGRHLAVLIALIISIS 312
           DTKGF L +SGV+ DIWTSYAARIM ISV+PF+IVQLPQML STSGR L+VLIALI+S+ 
Sbjct: 189 DTKGFHLKDSGVTVDIWTSYAARIMAISVIPFVIVQLPQMLGSTSGRQLSVLIALILSVL 248

Query: 313 MFVIYCLYQVFQPWIQRRKLAFVKHKHVIFGLLRHLKQQALGRLLTENGEPDKEIIDKLF 372
           M + YC+YQVFQPWIQRR+LAF KHKHVI G+LRHLKQ ALGRLL + G+PD+ +I KLF
Sbjct: 249 MLISYCVYQVFQPWIQRRRLAFAKHKHVISGILRHLKQHALGRLLDDEGQPDEHVIRKLF 308

Query: 373 SRIDENKDGRLSASELRALIVGIQFEELDLDHEDAVEKILNDFDTSRDSHVDATEFGNGI 432
             ID N DG LSA+EL+ALI+GI FE++D D +DAV K+L DFD + D  VD  EF  GI
Sbjct: 309 LTIDANNDGHLSAAELKALIIGISFEDIDFDKDDAVGKVLQDFDKTLDEQVDQEEFVRGI 368

Query: 433 IKWLSQVQGSRTGRADDGPHTMKFLHNFHQETKREHDLLDVGE--QSDEVVEDVEGGKGV 492
            +WL Q  G      + GP TMKFL NFH +TKREH LL   E  ++DE   +V   K +
Sbjct: 369 KQWLIQAMGGAPSGPEAGPRTMKFLDNFHVQTKREHALLGDNENGENDEEGGEVADPKWI 428

Query: 493 LIKAILFLLLGTAIAAAFADPLVDVVHNFSNATNIPAFFISFIALPLATNSSEAVSAIIF 552
            IKA L LLLG AIAAAFADPLVD V+NFS AT IP+FFISFIALPLATNSSEAVSAIIF
Sbjct: 429 TIKAALLLLLGAAIAAAFADPLVDTVNNFSAATGIPSFFISFIALPLATNSSEAVSAIIF 488

Query: 553 ASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVYMRGLVWNFSSEVLVILVVTVIMGL 612
           ASR K +TASLTFSEL G VTMNN+LCLSVFLA+VY+RGL WNFSSEVLVIL+V ++MG 
Sbjct: 489 ASRKKIRTASLTFSELCGGVTMNNILCLSVFLAIVYVRGLTWNFSSEVLVILIVCLVMGG 548

Query: 613 LGSFRTAFPLWTALVALLLYPLSLVLVYVLDYVFGWS 643
             SFRT +PLWT  +A LLYP SL LVY+LDY FGWS
Sbjct: 549 FASFRTTYPLWTCFIAYLLYPFSLGLVYILDYWFGWS 585

BLAST of CmaCh09G008300 vs. ExPASy Swiss-Prot
Match: Q5QNI2 (Sodium/calcium exchanger NCL1 OS=Oryza sativa subsp. japonica OX=39947 GN=NCL1 PE=2 SV=1)

HSP 1 Score: 659.4 bits (1700), Expect = 4.0e-188
Identity = 360/582 (61.86%), Postives = 454/582 (78.01%), Query Frame = 0

Query: 71  FLLCFLILCVQSHSSRFASISSSSDLVSDGVNELQEP--SYLRLNTLSSNS-------AP 130
           F L  L+L + + ++    IS  S   +   + L  P  + +RL T +S S       AP
Sbjct: 8   FPLVPLLLFLLAAAAYGRLISDGSPASASATSLLSNPVSAVIRLTTSNSASASSPPAAAP 67

Query: 131 EESCEQSYGFLPCTTSVLGNMFLIIVYGYLMFLAAKYLSAGSELLLEILGPGIVGGLFLP 190
           EE CEQSYGFLPCTT+VLGN+FL++ YG+LM+ AA +LSAGSELLLEI+GPG+VGGL LP
Sbjct: 68  EEKCEQSYGFLPCTTTVLGNLFLVLAYGFLMYKAATFLSAGSELLLEIMGPGLVGGLLLP 127

Query: 191 ALGALPDAMLILVSGLAGSAEVAQSQVSVGMGLLAGSTVMLLTLIWGTCVIVGKCDL-EG 250
            LGALPDA+L+LVSGL+GS E AQSQV +GMGLLAGSTV LLTL+WGTCV+VGKCD+   
Sbjct: 128 ILGALPDALLVLVSGLSGSRETAQSQVLIGMGLLAGSTVFLLTLLWGTCVVVGKCDIGPN 187

Query: 251 SVAIDSQDTKGFSLTESGVSTDIWTSYAARIMVISVVPFLIVQLPQMLNSTSGRHLAVLI 310
            VA+D Q+ KGFSLT +G+STD+ TSYAARIM ISV+PF+I Q P+ML +  G+ LAVL+
Sbjct: 188 GVAVDLQNNKGFSLTGTGISTDVQTSYAARIMGISVIPFIIAQFPKMLKTHHGQRLAVLL 247

Query: 311 ALIISISMFVIYCLYQVFQPWIQRRKLAFVKHKHVIFGLLRHLKQQALGRLLTENGEPDK 370
           ALI+S S+ + YCLYQVFQPWIQ+RKLA+ KHKHVI G+LRH + +ALGRLL E+G P++
Sbjct: 248 ALIVSFSLVLAYCLYQVFQPWIQKRKLAYAKHKHVISGILRHAQMEALGRLLNEDGTPNE 307

Query: 371 EIIDKLFSRIDENKDGRLSASELRALIVGIQFEELDLDHEDAVEKILNDFDTSRDSHVDA 430
           ++I KLF +ID ++   LS +EL ALI+GI FEE+D D  DAV+KI++DFDTS +  V+ 
Sbjct: 308 DVIKKLFHKIDMDESQTLSRAELHALIIGINFEEVDFDKNDAVDKIMDDFDTSGNDIVEE 367

Query: 431 TEFGNGIIKWLSQVQGSRTGRADDGPHTMKFLHNFHQETKREHDLLDVGEQSDEVVEDVE 490
            EF +G+ +WL++ + S       G ++ KF+ ++H  T++EHDLL   ++SDE VE VE
Sbjct: 368 AEFVSGMKRWLNEAKRS---VPTSGAYSNKFITDYHARTRQEHDLL--VDRSDETVESVE 427

Query: 491 GGKGVLIKAILFLLLGTAIAAAFADPLVDVVHNFSNATNIPAFFISFIALPLATNSSEAV 550
                + KA+  LLLG+AIAAAFADPLVD VHNFSNA++IP+FFISFIALPLATNSSEAV
Sbjct: 428 NPGWCITKAVGLLLLGSAIAAAFADPLVDAVHNFSNASHIPSFFISFIALPLATNSSEAV 487

Query: 551 SAIIFASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVYMRGLVWNFSSEVLVILVVT 610
           SAIIFASR K +T+SLTFSE+YG VTMNN LCL VFLAL+Y+R L W+FSSEVL+IL+V 
Sbjct: 488 SAIIFASRKKLRTSSLTFSEVYGGVTMNNTLCLGVFLALIYIRNLTWDFSSEVLIILLVC 547

Query: 611 VIMGLLGSFRTAFPLWTALVALLLYPLSLVLVYVLDYVFGWS 643
           VIMGL  SFRT FPLWT LVA +LYPLSLV+VY+LD+VFGWS
Sbjct: 548 VIMGLFTSFRTTFPLWTCLVAYMLYPLSLVVVYILDFVFGWS 584

BLAST of CmaCh09G008300 vs. ExPASy Swiss-Prot
Match: Q6K3R5 (Sodium/calcium exchanger NCL2 OS=Oryza sativa subsp. japonica OX=39947 GN=NCL2 PE=2 SV=2)

HSP 1 Score: 626.7 bits (1615), Expect = 2.9e-178
Identity = 344/581 (59.21%), Postives = 439/581 (75.56%), Query Frame = 0

Query: 67  LHLVFLLCFLILCVQSHSSRFASISSSSDLVSDG--VNELQEPSYLRLNTLSSNSAPEES 126
           L ++ LL  ++  + +H  R          +SDG   N     S LRL + ++ +  +  
Sbjct: 11  LPVLLLLLLVVAPLLAHGRR--------PFISDGGNANANANASVLRLPSAAAAAGEDMG 70

Query: 127 CEQSYGFLPCTTSVLGNMFLIIVYGYLMFLAAKYLSAGSELLLEILGPGIVGGLFLPALG 186
           CE SYGFLPCTT+  GN+FL++ YG+LMF +A YLS+GSE+LL+ILGPGIVGGLFLP LG
Sbjct: 71  CEMSYGFLPCTTTAWGNLFLVLAYGFLMFKSATYLSSGSEMLLQILGPGIVGGLFLPILG 130

Query: 187 ALPDAMLILVSGLAGSAEVAQSQVSVGMGLLAGSTVMLLTLIWGTCVIVGKCDL-EGSVA 246
           ALPDA+LILVSGL+G+ EVAQSQV +GMGLLAGSTVMLLTL+WG+CV+VGKCDL E S A
Sbjct: 131 ALPDALLILVSGLSGTKEVAQSQVLIGMGLLAGSTVMLLTLLWGSCVVVGKCDLSENSTA 190

Query: 247 IDSQDTKGFSLTESGVSTDIWTSYAARIMVISVVPFLIVQLPQMLNSTSGRHLAVLIALI 306
           IDS+DTKGFSL  SGVSTD  TSYAARIM IS++PF+IVQ+P++    SG  + VLI LI
Sbjct: 191 IDSRDTKGFSLLGSGVSTDKQTSYAARIMAISILPFIIVQIPKIFKLHSGHQITVLIGLI 250

Query: 307 ISISMFVIYCLYQVFQPWIQRRKLAFVKHKHVIFGLLRHLKQQALGRLLTENGEPDKEII 366
           ++  + + YCLYQVFQPWIQRR+L + + KHV+ GLLRH ++ ++GRLL + G P+  +I
Sbjct: 251 VAALLLLSYCLYQVFQPWIQRRRLEYTRLKHVMSGLLRHAQKHSIGRLLDDEGRPNVSVI 310

Query: 367 DKLFSRIDENKDGRLSASELRALIVGIQFEELDLDHEDAVEKILNDFDTSRDSHVDATEF 426
           +KLF RID++ DG+L   EL+A IVGI FE++D +   A ++++ DFDTSR+  ++  EF
Sbjct: 311 EKLFHRIDQDNDGKLERGELQAFIVGINFEDIDWNSNLAADQVMADFDTSRNHFIEKGEF 370

Query: 427 GNGIIKWLSQVQGSRTGRADDGPHTMKFLHNFHQETKREH-DLLDVGEQSDEVVEDVEGG 486
            NG+++WL + + + T     G ++ KFL++FH  T+ E   LLD  E+  E     +G 
Sbjct: 371 VNGMLRWLDEAKRTVT----SGAYSKKFLNDFHARTRDEQTGLLDKDEEEGE----ADGN 430

Query: 487 -KGVLIKAILFLLLGTAIAAAFADPLVDVVHNFSNATNIPAFFISFIALPLATNSSEAVS 546
                IKAIL LLLGTA+AAA ADPLVD VHNFSNAT+IP+FFISFI +PLATNSSEAVS
Sbjct: 431 PTWTCIKAILLLLLGTAMAAASADPLVDAVHNFSNATHIPSFFISFIVMPLATNSSEAVS 490

Query: 547 AIIFASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVYMRGLVWNFSSEVLVILVVTV 606
           AIIFASR K++T SLTFSE+YG VTMNN LCL+VFLALVY+RGL W+FSSEVL+IL+V +
Sbjct: 491 AIIFASRKKKRTLSLTFSEVYGGVTMNNTLCLAVFLALVYVRGLTWDFSSEVLIILLVCI 550

Query: 607 IMGLLGSFRTAFPLWTALVALLLYPLSLVLVYVLDYVFGWS 643
           IMGL  SFRT FPLWT  VA LLYPLSL++VY+LDY FGWS
Sbjct: 551 IMGLFTSFRTDFPLWTCFVAFLLYPLSLIMVYILDYKFGWS 575

BLAST of CmaCh09G008300 vs. ExPASy Swiss-Prot
Match: Q5KQN0 (Vacuolar cation/proton exchanger 2 OS=Oryza sativa subsp. japonica OX=39947 GN=CAX2 PE=2 SV=2)

HSP 1 Score: 50.1 bits (118), Expect = 1.1e-04
Identity = 43/139 (30.94%), Postives = 72/139 (51.80%), Query Frame = 0

Query: 468 VGEQSDEVVEDVEGGKGVLI-KAILFLLLGTAIAAAFADPLVDVVHNFSNATNIPAFFIS 527
           +GEQ +EV ED E  K +   +AI +L + T   +  +  LVD +   S + N+P  FIS
Sbjct: 261 IGEQEEEVTEDEEEEKEITQGEAICWLFVLTIWISILSGYLVDAIQGASESLNMPVAFIS 320

Query: 528 FIALPLATNSSEAVSAIIFASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVYMRG-- 587
            I LP+  N++E  SAI+FA +DK     +T     G+ T  ++  +   + + ++ G  
Sbjct: 321 VILLPIVGNAAEHASAIMFAMKDK---LDITLGVAIGSSTQISMFVIPFCVVIGWIMGQQ 380

Query: 588 LVWNFSSEVLVILVVTVIM 604
           +  NF       L +TV++
Sbjct: 381 MDLNFQLFETATLFITVLV 396

BLAST of CmaCh09G008300 vs. ExPASy TrEMBL
Match: A0A6J1IGZ1 (sodium/calcium exchanger NCL-like OS=Cucurbita maxima OX=3661 GN=LOC111476856 PE=4 SV=1)

HSP 1 Score: 1088.9 bits (2815), Expect = 0.0e+00
Identity = 581/581 (100.00%), Postives = 581/581 (100.00%), Query Frame = 0

Query: 62  MSRTPLHLVFLLCFLILCVQSHSSRFASISSSSDLVSDGVNELQEPSYLRLNTLSSNSAP 121
           MSRTPLHLVFLLCFLILCVQSHSSRFASISSSSDLVSDGVNELQEPSYLRLNTLSSNSAP
Sbjct: 1   MSRTPLHLVFLLCFLILCVQSHSSRFASISSSSDLVSDGVNELQEPSYLRLNTLSSNSAP 60

Query: 122 EESCEQSYGFLPCTTSVLGNMFLIIVYGYLMFLAAKYLSAGSELLLEILGPGIVGGLFLP 181
           EESCEQSYGFLPCTTSVLGNMFLIIVYGYLMFLAAKYLSAGSELLLEILGPGIVGGLFLP
Sbjct: 61  EESCEQSYGFLPCTTSVLGNMFLIIVYGYLMFLAAKYLSAGSELLLEILGPGIVGGLFLP 120

Query: 182 ALGALPDAMLILVSGLAGSAEVAQSQVSVGMGLLAGSTVMLLTLIWGTCVIVGKCDLEGS 241
           ALGALPDAMLILVSGLAGSAEVAQSQVSVGMGLLAGSTVMLLTLIWGTCVIVGKCDLEGS
Sbjct: 121 ALGALPDAMLILVSGLAGSAEVAQSQVSVGMGLLAGSTVMLLTLIWGTCVIVGKCDLEGS 180

Query: 242 VAIDSQDTKGFSLTESGVSTDIWTSYAARIMVISVVPFLIVQLPQMLNSTSGRHLAVLIA 301
           VAIDSQDTKGFSLTESGVSTDIWTSYAARIMVISVVPFLIVQLPQMLNSTSGRHLAVLIA
Sbjct: 181 VAIDSQDTKGFSLTESGVSTDIWTSYAARIMVISVVPFLIVQLPQMLNSTSGRHLAVLIA 240

Query: 302 LIISISMFVIYCLYQVFQPWIQRRKLAFVKHKHVIFGLLRHLKQQALGRLLTENGEPDKE 361
           LIISISMFVIYCLYQVFQPWIQRRKLAFVKHKHVIFGLLRHLKQQALGRLLTENGEPDKE
Sbjct: 241 LIISISMFVIYCLYQVFQPWIQRRKLAFVKHKHVIFGLLRHLKQQALGRLLTENGEPDKE 300

Query: 362 IIDKLFSRIDENKDGRLSASELRALIVGIQFEELDLDHEDAVEKILNDFDTSRDSHVDAT 421
           IIDKLFSRIDENKDGRLSASELRALIVGIQFEELDLDHEDAVEKILNDFDTSRDSHVDAT
Sbjct: 301 IIDKLFSRIDENKDGRLSASELRALIVGIQFEELDLDHEDAVEKILNDFDTSRDSHVDAT 360

Query: 422 EFGNGIIKWLSQVQGSRTGRADDGPHTMKFLHNFHQETKREHDLLDVGEQSDEVVEDVEG 481
           EFGNGIIKWLSQVQGSRTGRADDGPHTMKFLHNFHQETKREHDLLDVGEQSDEVVEDVEG
Sbjct: 361 EFGNGIIKWLSQVQGSRTGRADDGPHTMKFLHNFHQETKREHDLLDVGEQSDEVVEDVEG 420

Query: 482 GKGVLIKAILFLLLGTAIAAAFADPLVDVVHNFSNATNIPAFFISFIALPLATNSSEAVS 541
           GKGVLIKAILFLLLGTAIAAAFADPLVDVVHNFSNATNIPAFFISFIALPLATNSSEAVS
Sbjct: 421 GKGVLIKAILFLLLGTAIAAAFADPLVDVVHNFSNATNIPAFFISFIALPLATNSSEAVS 480

Query: 542 AIIFASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVYMRGLVWNFSSEVLVILVVTV 601
           AIIFASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVYMRGLVWNFSSEVLVILVVTV
Sbjct: 481 AIIFASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVYMRGLVWNFSSEVLVILVVTV 540

Query: 602 IMGLLGSFRTAFPLWTALVALLLYPLSLVLVYVLDYVFGWS 643
           IMGLLGSFRTAFPLWTALVALLLYPLSLVLVYVLDYVFGWS
Sbjct: 541 IMGLLGSFRTAFPLWTALVALLLYPLSLVLVYVLDYVFGWS 581

BLAST of CmaCh09G008300 vs. ExPASy TrEMBL
Match: A0A6J1FAG1 (sodium/calcium exchanger NCL-like OS=Cucurbita moschata OX=3662 GN=LOC111443554 PE=4 SV=1)

HSP 1 Score: 1082.4 bits (2798), Expect = 0.0e+00
Identity = 576/581 (99.14%), Postives = 579/581 (99.66%), Query Frame = 0

Query: 62  MSRTPLHLVFLLCFLILCVQSHSSRFASISSSSDLVSDGVNELQEPSYLRLNTLSSNSAP 121
           MSRTPLHLVFLLCFLILCVQSHSSRFASISSSSDLVSDGVN+LQEPSYLRLNTL SNSAP
Sbjct: 1   MSRTPLHLVFLLCFLILCVQSHSSRFASISSSSDLVSDGVNDLQEPSYLRLNTLPSNSAP 60

Query: 122 EESCEQSYGFLPCTTSVLGNMFLIIVYGYLMFLAAKYLSAGSELLLEILGPGIVGGLFLP 181
           EE+CEQSYGFLPCTTSVLGNMFLIIVYGYLMFLAAKYLSAGSELLLEILGPGIVGGLFLP
Sbjct: 61  EEACEQSYGFLPCTTSVLGNMFLIIVYGYLMFLAAKYLSAGSELLLEILGPGIVGGLFLP 120

Query: 182 ALGALPDAMLILVSGLAGSAEVAQSQVSVGMGLLAGSTVMLLTLIWGTCVIVGKCDLEGS 241
           ALGALPDAMLILVSGLAGSAEVAQSQVSVGMGLLAGSTVMLLTLIWGTCVIVGKCDLEGS
Sbjct: 121 ALGALPDAMLILVSGLAGSAEVAQSQVSVGMGLLAGSTVMLLTLIWGTCVIVGKCDLEGS 180

Query: 242 VAIDSQDTKGFSLTESGVSTDIWTSYAARIMVISVVPFLIVQLPQMLNSTSGRHLAVLIA 301
           VAIDSQDTKGFSLTESGVSTDIWTSYAARIMVISV PFLIVQLPQMLNSTSGRHLAVLIA
Sbjct: 181 VAIDSQDTKGFSLTESGVSTDIWTSYAARIMVISVFPFLIVQLPQMLNSTSGRHLAVLIA 240

Query: 302 LIISISMFVIYCLYQVFQPWIQRRKLAFVKHKHVIFGLLRHLKQQALGRLLTENGEPDKE 361
           LIISISMF+IYCLYQVFQPWIQRRKLAFVKHKHVIFGLLRHLKQQALGRLLTENGEPDKE
Sbjct: 241 LIISISMFIIYCLYQVFQPWIQRRKLAFVKHKHVIFGLLRHLKQQALGRLLTENGEPDKE 300

Query: 362 IIDKLFSRIDENKDGRLSASELRALIVGIQFEELDLDHEDAVEKILNDFDTSRDSHVDAT 421
           IIDKLFSRIDENKDGRLSASELRALIVGIQFEELDLDHEDAVEKILNDFDTSRDSHVDAT
Sbjct: 301 IIDKLFSRIDENKDGRLSASELRALIVGIQFEELDLDHEDAVEKILNDFDTSRDSHVDAT 360

Query: 422 EFGNGIIKWLSQVQGSRTGRADDGPHTMKFLHNFHQETKREHDLLDVGEQSDEVVEDVEG 481
           EFGNGIIKWLSQVQGSRTGRADDGPHTMKFLHNFHQETKREHDLLDVGEQSDEVVEDVEG
Sbjct: 361 EFGNGIIKWLSQVQGSRTGRADDGPHTMKFLHNFHQETKREHDLLDVGEQSDEVVEDVEG 420

Query: 482 GKGVLIKAILFLLLGTAIAAAFADPLVDVVHNFSNATNIPAFFISFIALPLATNSSEAVS 541
           GKGVLIKAILFLLLGTAIAAAFADPLVDVVHNFSNATNIPAFFISFIALPLATNSSEAVS
Sbjct: 421 GKGVLIKAILFLLLGTAIAAAFADPLVDVVHNFSNATNIPAFFISFIALPLATNSSEAVS 480

Query: 542 AIIFASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVYMRGLVWNFSSEVLVILVVTV 601
           AIIFASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVYMRGLVWNFSSEVLVILVVTV
Sbjct: 481 AIIFASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVYMRGLVWNFSSEVLVILVVTV 540

Query: 602 IMGLLGSFRTAFPLWTALVALLLYPLSLVLVYVLDYVFGWS 643
           IMGLLGSFRTAFPLWTALVALLLYPLSLVLVYVLDYVFGWS
Sbjct: 541 IMGLLGSFRTAFPLWTALVALLLYPLSLVLVYVLDYVFGWS 581

BLAST of CmaCh09G008300 vs. ExPASy TrEMBL
Match: A0A6J1FT50 (sodium/calcium exchanger NCL-like OS=Cucurbita moschata OX=3662 GN=LOC111446626 PE=4 SV=1)

HSP 1 Score: 1006.9 bits (2602), Expect = 3.8e-290
Identity = 533/581 (91.74%), Postives = 561/581 (96.56%), Query Frame = 0

Query: 62  MSRTPLHLVFLLCFLILCVQSHSSRFASISSSSDLVSDGVNELQEPSYLRLNTLSSNSAP 121
           MSR PL+L+FLL F ILC QSHSSRFASISSSSDLVSDGVNELQE  YLRLN+L S SAP
Sbjct: 1   MSRPPLYLIFLLIFFILCGQSHSSRFASISSSSDLVSDGVNELQETPYLRLNSLESVSAP 60

Query: 122 EESCEQSYGFLPCTTSVLGNMFLIIVYGYLMFLAAKYLSAGSELLLEILGPGIVGGLFLP 181
           EESCE+SYGFLPCTTS LGN+FLIIVYGYLMFLAAKYLS GSELLLEILGPGIVGGLFLP
Sbjct: 61  EESCEESYGFLPCTTSALGNLFLIIVYGYLMFLAAKYLSTGSELLLEILGPGIVGGLFLP 120

Query: 182 ALGALPDAMLILVSGLAGSAEVAQSQVSVGMGLLAGSTVMLLTLIWGTCVIVGKCDLEGS 241
           ALGALPDAMLILVSGLAGSAEVAQSQVSVGMGLLAGSTVMLLTLIWGTCVIVGKCDL+ S
Sbjct: 121 ALGALPDAMLILVSGLAGSAEVAQSQVSVGMGLLAGSTVMLLTLIWGTCVIVGKCDLQDS 180

Query: 242 VAIDSQDTKGFSLTESGVSTDIWTSYAARIMVISVVPFLIVQLPQMLNSTSGRHLAVLIA 301
           VAID QDTKGFSLTESGVSTDIWTSYAARIMVISVVPFL+VQLPQ+LNSTSGRHLAVLIA
Sbjct: 181 VAIDLQDTKGFSLTESGVSTDIWTSYAARIMVISVVPFLVVQLPQLLNSTSGRHLAVLIA 240

Query: 302 LIISISMFVIYCLYQVFQPWIQRRKLAFVKHKHVIFGLLRHLKQQALGRLLTENGEPDKE 361
           LIIS+SMF+IYCLYQVFQPWIQRRKLAFVKHKHVIFGLLRHLKQQALGRLLTE+GEPD+E
Sbjct: 241 LIISVSMFIIYCLYQVFQPWIQRRKLAFVKHKHVIFGLLRHLKQQALGRLLTESGEPDRE 300

Query: 362 IIDKLFSRIDENKDGRLSASELRALIVGIQFEELDLDHEDAVEKILNDFDTSRDSHVDAT 421
           II+KLFSRID NK+G LSASELRALIVGIQFE +DLDH+DAV KIL+DFDTSRD+HVD+T
Sbjct: 301 IIEKLFSRIDANKNGLLSASELRALIVGIQFEAIDLDHDDAVNKILSDFDTSRDNHVDST 360

Query: 422 EFGNGIIKWLSQVQGSRTGRADDGPHTMKFLHNFHQETKREHDLLDVGEQSDEVVEDVEG 481
           EF NGII+WL+QVQG+RTGRADDGPHT+K+LHNFHQETKREHDLLDVGEQSDEVVE VE 
Sbjct: 361 EFANGIIRWLAQVQGARTGRADDGPHTLKYLHNFHQETKREHDLLDVGEQSDEVVEGVEE 420

Query: 482 GKGVLIKAILFLLLGTAIAAAFADPLVDVVHNFSNATNIPAFFISFIALPLATNSSEAVS 541
            KGVLI+AILFLLLGTAIAAAFADPLVDVVHNFSNAT+IPAFFISFIALPLATNSSEAVS
Sbjct: 421 SKGVLIRAILFLLLGTAIAAAFADPLVDVVHNFSNATSIPAFFISFIALPLATNSSEAVS 480

Query: 542 AIIFASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVYMRGLVWNFSSEVLVILVVTV 601
           AIIFASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVY+RGLVWNFSSEVLVILVVT+
Sbjct: 481 AIIFASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVYLRGLVWNFSSEVLVILVVTM 540

Query: 602 IMGLLGSFRTAFPLWTALVALLLYPLSLVLVYVLDYVFGWS 643
           IMGLLGSFRTAFPLWTALVALLLYPLSLVLVYVLDYVFGWS
Sbjct: 541 IMGLLGSFRTAFPLWTALVALLLYPLSLVLVYVLDYVFGWS 581

BLAST of CmaCh09G008300 vs. ExPASy TrEMBL
Match: A0A5A7VFE4 (Sodium/calcium exchanger family protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold384G001180 PE=4 SV=1)

HSP 1 Score: 1005.7 bits (2599), Expect = 8.4e-290
Identity = 536/590 (90.85%), Postives = 561/590 (95.08%), Query Frame = 0

Query: 62  MSRTPLHLVFLLCFLILCVQSHSSRFASI--------SSSSDLVSDGVNELQEPSYLRLN 121
           MS++PLHL+FLL FL+LC QS+SSR  SI        SSSSDLVSDGVNELQEPSYL LN
Sbjct: 1   MSKSPLHLLFLLFFLVLCSQSYSSRLPSISSLTSSSSSSSSDLVSDGVNELQEPSYLHLN 60

Query: 122 TLSSNSAP-EESCEQSYGFLPCTTSVLGNMFLIIVYGYLMFLAAKYLSAGSELLLEILGP 181
           TLSS SAP EESCEQSYGFLPCTT+ LGN+FLIIVYGYLMFLAAKYLS GSELLLEILGP
Sbjct: 61  TLSSFSAPEEESCEQSYGFLPCTTTALGNLFLIIVYGYLMFLAAKYLSTGSELLLEILGP 120

Query: 182 GIVGGLFLPALGALPDAMLILVSGLAGSAEVAQSQVSVGMGLLAGSTVMLLTLIWGTCVI 241
           GIVGGLFLPALGALPDAMLILVSGLAGSAEVAQSQVSVGMGLLAGSTVMLLTLIWGTCVI
Sbjct: 121 GIVGGLFLPALGALPDAMLILVSGLAGSAEVAQSQVSVGMGLLAGSTVMLLTLIWGTCVI 180

Query: 242 VGKCDLEGSVAIDSQDTKGFSLTESGVSTDIWTSYAARIMVISVVPFLIVQLPQMLNSTS 301
           VGKCDL+ SVAIDSQDTKGFSLTESGVSTDIWTSYAARIMVISVVPFLIVQLPQMLNSTS
Sbjct: 181 VGKCDLQDSVAIDSQDTKGFSLTESGVSTDIWTSYAARIMVISVVPFLIVQLPQMLNSTS 240

Query: 302 GRHLAVLIALIISISMFVIYCLYQVFQPWIQRRKLAFVKHKHVIFGLLRHLKQQALGRLL 361
           GRHLAVLIALI+S+SMF+ YCLYQVFQPWIQRRKLAFVKHKHVIFG LRHLKQQ LGRLL
Sbjct: 241 GRHLAVLIALIVSVSMFITYCLYQVFQPWIQRRKLAFVKHKHVIFGFLRHLKQQTLGRLL 300

Query: 362 TENGEPDKEIIDKLFSRIDENKDGRLSASELRALIVGIQFEELDLDHEDAVEKILNDFDT 421
           TENGEPDKEII+KLFSRID NKDG LSASELRALIVGIQF+E+DLDH+DAV+KI+NDFDT
Sbjct: 301 TENGEPDKEIIEKLFSRIDVNKDGLLSASELRALIVGIQFDEIDLDHDDAVDKIMNDFDT 360

Query: 422 SRDSHVDATEFGNGIIKWLSQVQGSRTGRADDGPHTMKFLHNFHQETKREHDLLDVGEQS 481
           SRDSHVD+ EFGNGII+WLSQVQGSRTGR DDGPHTMK+LHNFHQETKREHDLLDVGEQS
Sbjct: 361 SRDSHVDSNEFGNGIIRWLSQVQGSRTGRGDDGPHTMKYLHNFHQETKREHDLLDVGEQS 420

Query: 482 DEVVEDVEGGKGVLIKAILFLLLGTAIAAAFADPLVDVVHNFSNATNIPAFFISFIALPL 541
           DEVVE VE GKGVLIKAILFLLLGTAIAAAFADPLVDVVHNFSNAT IPAFFISFIALPL
Sbjct: 421 DEVVEGVEEGKGVLIKAILFLLLGTAIAAAFADPLVDVVHNFSNATKIPAFFISFIALPL 480

Query: 542 ATNSSEAVSAIIFASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVYMRGLVWNFSSE 601
           ATNSSEAVSAIIFASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVY+RGLVWNFSSE
Sbjct: 481 ATNSSEAVSAIIFASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVYLRGLVWNFSSE 540

Query: 602 VLVILVVTVIMGLLGSFRTAFPLWTALVALLLYPLSLVLVYVLDYVFGWS 643
           VLVILVVTV+MG++GSFRTAFPLWT+LVALLLYP SLVLVYVLDYVFGWS
Sbjct: 541 VLVILVVTVVMGVMGSFRTAFPLWTSLVALLLYPFSLVLVYVLDYVFGWS 590

BLAST of CmaCh09G008300 vs. ExPASy TrEMBL
Match: A0A1S3CJX3 (uncharacterized protein LOC103501799 OS=Cucumis melo OX=3656 GN=LOC103501799 PE=4 SV=1)

HSP 1 Score: 1005.7 bits (2599), Expect = 8.4e-290
Identity = 536/590 (90.85%), Postives = 561/590 (95.08%), Query Frame = 0

Query: 62  MSRTPLHLVFLLCFLILCVQSHSSRFASI--------SSSSDLVSDGVNELQEPSYLRLN 121
           MS++PLHL+FLL FL+LC QS+SSR  SI        SSSSDLVSDGVNELQEPSYL LN
Sbjct: 1   MSKSPLHLLFLLFFLVLCSQSYSSRLPSISSLTSSSSSSSSDLVSDGVNELQEPSYLHLN 60

Query: 122 TLSSNSAP-EESCEQSYGFLPCTTSVLGNMFLIIVYGYLMFLAAKYLSAGSELLLEILGP 181
           TLSS SAP EESCEQSYGFLPCTT+ LGN+FLIIVYGYLMFLAAKYLS GSELLLEILGP
Sbjct: 61  TLSSFSAPEEESCEQSYGFLPCTTTALGNLFLIIVYGYLMFLAAKYLSTGSELLLEILGP 120

Query: 182 GIVGGLFLPALGALPDAMLILVSGLAGSAEVAQSQVSVGMGLLAGSTVMLLTLIWGTCVI 241
           GIVGGLFLPALGALPDAMLILVSGLAGSAEVAQSQVSVGMGLLAGSTVMLLTLIWGTCVI
Sbjct: 121 GIVGGLFLPALGALPDAMLILVSGLAGSAEVAQSQVSVGMGLLAGSTVMLLTLIWGTCVI 180

Query: 242 VGKCDLEGSVAIDSQDTKGFSLTESGVSTDIWTSYAARIMVISVVPFLIVQLPQMLNSTS 301
           VGKCDL+ SVAIDSQDTKGFSLTESGVSTDIWTSYAARIMVISVVPFLIVQLPQMLNSTS
Sbjct: 181 VGKCDLQDSVAIDSQDTKGFSLTESGVSTDIWTSYAARIMVISVVPFLIVQLPQMLNSTS 240

Query: 302 GRHLAVLIALIISISMFVIYCLYQVFQPWIQRRKLAFVKHKHVIFGLLRHLKQQALGRLL 361
           GRHLAVLIALI+S+SMF+ YCLYQVFQPWIQRRKLAFVKHKHVIFG LRHLKQQ LGRLL
Sbjct: 241 GRHLAVLIALIVSVSMFITYCLYQVFQPWIQRRKLAFVKHKHVIFGFLRHLKQQTLGRLL 300

Query: 362 TENGEPDKEIIDKLFSRIDENKDGRLSASELRALIVGIQFEELDLDHEDAVEKILNDFDT 421
           TENGEPDKEII+KLFSRID NKDG LSASELRALIVGIQF+E+DLDH+DAV+KI+NDFDT
Sbjct: 301 TENGEPDKEIIEKLFSRIDVNKDGLLSASELRALIVGIQFDEIDLDHDDAVDKIMNDFDT 360

Query: 422 SRDSHVDATEFGNGIIKWLSQVQGSRTGRADDGPHTMKFLHNFHQETKREHDLLDVGEQS 481
           SRDSHVD+ EFGNGII+WLSQVQGSRTGR DDGPHTMK+LHNFHQETKREHDLLDVGEQS
Sbjct: 361 SRDSHVDSNEFGNGIIRWLSQVQGSRTGRGDDGPHTMKYLHNFHQETKREHDLLDVGEQS 420

Query: 482 DEVVEDVEGGKGVLIKAILFLLLGTAIAAAFADPLVDVVHNFSNATNIPAFFISFIALPL 541
           DEVVE VE GKGVLIKAILFLLLGTAIAAAFADPLVDVVHNFSNAT IPAFFISFIALPL
Sbjct: 421 DEVVEGVEEGKGVLIKAILFLLLGTAIAAAFADPLVDVVHNFSNATKIPAFFISFIALPL 480

Query: 542 ATNSSEAVSAIIFASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVYMRGLVWNFSSE 601
           ATNSSEAVSAIIFASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVY+RGLVWNFSSE
Sbjct: 481 ATNSSEAVSAIIFASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVYLRGLVWNFSSE 540

Query: 602 VLVILVVTVIMGLLGSFRTAFPLWTALVALLLYPLSLVLVYVLDYVFGWS 643
           VLVILVVTV+MG++GSFRTAFPLWT+LVALLLYP SLVLVYVLDYVFGWS
Sbjct: 541 VLVILVVTVVMGVMGSFRTAFPLWTSLVALLLYPFSLVLVYVLDYVFGWS 590

BLAST of CmaCh09G008300 vs. NCBI nr
Match: XP_022976461.1 (sodium/calcium exchanger NCL-like [Cucurbita maxima])

HSP 1 Score: 1088.9 bits (2815), Expect = 0.0e+00
Identity = 581/581 (100.00%), Postives = 581/581 (100.00%), Query Frame = 0

Query: 62  MSRTPLHLVFLLCFLILCVQSHSSRFASISSSSDLVSDGVNELQEPSYLRLNTLSSNSAP 121
           MSRTPLHLVFLLCFLILCVQSHSSRFASISSSSDLVSDGVNELQEPSYLRLNTLSSNSAP
Sbjct: 1   MSRTPLHLVFLLCFLILCVQSHSSRFASISSSSDLVSDGVNELQEPSYLRLNTLSSNSAP 60

Query: 122 EESCEQSYGFLPCTTSVLGNMFLIIVYGYLMFLAAKYLSAGSELLLEILGPGIVGGLFLP 181
           EESCEQSYGFLPCTTSVLGNMFLIIVYGYLMFLAAKYLSAGSELLLEILGPGIVGGLFLP
Sbjct: 61  EESCEQSYGFLPCTTSVLGNMFLIIVYGYLMFLAAKYLSAGSELLLEILGPGIVGGLFLP 120

Query: 182 ALGALPDAMLILVSGLAGSAEVAQSQVSVGMGLLAGSTVMLLTLIWGTCVIVGKCDLEGS 241
           ALGALPDAMLILVSGLAGSAEVAQSQVSVGMGLLAGSTVMLLTLIWGTCVIVGKCDLEGS
Sbjct: 121 ALGALPDAMLILVSGLAGSAEVAQSQVSVGMGLLAGSTVMLLTLIWGTCVIVGKCDLEGS 180

Query: 242 VAIDSQDTKGFSLTESGVSTDIWTSYAARIMVISVVPFLIVQLPQMLNSTSGRHLAVLIA 301
           VAIDSQDTKGFSLTESGVSTDIWTSYAARIMVISVVPFLIVQLPQMLNSTSGRHLAVLIA
Sbjct: 181 VAIDSQDTKGFSLTESGVSTDIWTSYAARIMVISVVPFLIVQLPQMLNSTSGRHLAVLIA 240

Query: 302 LIISISMFVIYCLYQVFQPWIQRRKLAFVKHKHVIFGLLRHLKQQALGRLLTENGEPDKE 361
           LIISISMFVIYCLYQVFQPWIQRRKLAFVKHKHVIFGLLRHLKQQALGRLLTENGEPDKE
Sbjct: 241 LIISISMFVIYCLYQVFQPWIQRRKLAFVKHKHVIFGLLRHLKQQALGRLLTENGEPDKE 300

Query: 362 IIDKLFSRIDENKDGRLSASELRALIVGIQFEELDLDHEDAVEKILNDFDTSRDSHVDAT 421
           IIDKLFSRIDENKDGRLSASELRALIVGIQFEELDLDHEDAVEKILNDFDTSRDSHVDAT
Sbjct: 301 IIDKLFSRIDENKDGRLSASELRALIVGIQFEELDLDHEDAVEKILNDFDTSRDSHVDAT 360

Query: 422 EFGNGIIKWLSQVQGSRTGRADDGPHTMKFLHNFHQETKREHDLLDVGEQSDEVVEDVEG 481
           EFGNGIIKWLSQVQGSRTGRADDGPHTMKFLHNFHQETKREHDLLDVGEQSDEVVEDVEG
Sbjct: 361 EFGNGIIKWLSQVQGSRTGRADDGPHTMKFLHNFHQETKREHDLLDVGEQSDEVVEDVEG 420

Query: 482 GKGVLIKAILFLLLGTAIAAAFADPLVDVVHNFSNATNIPAFFISFIALPLATNSSEAVS 541
           GKGVLIKAILFLLLGTAIAAAFADPLVDVVHNFSNATNIPAFFISFIALPLATNSSEAVS
Sbjct: 421 GKGVLIKAILFLLLGTAIAAAFADPLVDVVHNFSNATNIPAFFISFIALPLATNSSEAVS 480

Query: 542 AIIFASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVYMRGLVWNFSSEVLVILVVTV 601
           AIIFASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVYMRGLVWNFSSEVLVILVVTV
Sbjct: 481 AIIFASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVYMRGLVWNFSSEVLVILVVTV 540

Query: 602 IMGLLGSFRTAFPLWTALVALLLYPLSLVLVYVLDYVFGWS 643
           IMGLLGSFRTAFPLWTALVALLLYPLSLVLVYVLDYVFGWS
Sbjct: 541 IMGLLGSFRTAFPLWTALVALLLYPLSLVLVYVLDYVFGWS 581

BLAST of CmaCh09G008300 vs. NCBI nr
Match: XP_022937179.1 (sodium/calcium exchanger NCL-like [Cucurbita moschata] >KAG7024799.1 Sodium/calcium exchanger NCL [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1082.4 bits (2798), Expect = 0.0e+00
Identity = 576/581 (99.14%), Postives = 579/581 (99.66%), Query Frame = 0

Query: 62  MSRTPLHLVFLLCFLILCVQSHSSRFASISSSSDLVSDGVNELQEPSYLRLNTLSSNSAP 121
           MSRTPLHLVFLLCFLILCVQSHSSRFASISSSSDLVSDGVN+LQEPSYLRLNTL SNSAP
Sbjct: 1   MSRTPLHLVFLLCFLILCVQSHSSRFASISSSSDLVSDGVNDLQEPSYLRLNTLPSNSAP 60

Query: 122 EESCEQSYGFLPCTTSVLGNMFLIIVYGYLMFLAAKYLSAGSELLLEILGPGIVGGLFLP 181
           EE+CEQSYGFLPCTTSVLGNMFLIIVYGYLMFLAAKYLSAGSELLLEILGPGIVGGLFLP
Sbjct: 61  EEACEQSYGFLPCTTSVLGNMFLIIVYGYLMFLAAKYLSAGSELLLEILGPGIVGGLFLP 120

Query: 182 ALGALPDAMLILVSGLAGSAEVAQSQVSVGMGLLAGSTVMLLTLIWGTCVIVGKCDLEGS 241
           ALGALPDAMLILVSGLAGSAEVAQSQVSVGMGLLAGSTVMLLTLIWGTCVIVGKCDLEGS
Sbjct: 121 ALGALPDAMLILVSGLAGSAEVAQSQVSVGMGLLAGSTVMLLTLIWGTCVIVGKCDLEGS 180

Query: 242 VAIDSQDTKGFSLTESGVSTDIWTSYAARIMVISVVPFLIVQLPQMLNSTSGRHLAVLIA 301
           VAIDSQDTKGFSLTESGVSTDIWTSYAARIMVISV PFLIVQLPQMLNSTSGRHLAVLIA
Sbjct: 181 VAIDSQDTKGFSLTESGVSTDIWTSYAARIMVISVFPFLIVQLPQMLNSTSGRHLAVLIA 240

Query: 302 LIISISMFVIYCLYQVFQPWIQRRKLAFVKHKHVIFGLLRHLKQQALGRLLTENGEPDKE 361
           LIISISMF+IYCLYQVFQPWIQRRKLAFVKHKHVIFGLLRHLKQQALGRLLTENGEPDKE
Sbjct: 241 LIISISMFIIYCLYQVFQPWIQRRKLAFVKHKHVIFGLLRHLKQQALGRLLTENGEPDKE 300

Query: 362 IIDKLFSRIDENKDGRLSASELRALIVGIQFEELDLDHEDAVEKILNDFDTSRDSHVDAT 421
           IIDKLFSRIDENKDGRLSASELRALIVGIQFEELDLDHEDAVEKILNDFDTSRDSHVDAT
Sbjct: 301 IIDKLFSRIDENKDGRLSASELRALIVGIQFEELDLDHEDAVEKILNDFDTSRDSHVDAT 360

Query: 422 EFGNGIIKWLSQVQGSRTGRADDGPHTMKFLHNFHQETKREHDLLDVGEQSDEVVEDVEG 481
           EFGNGIIKWLSQVQGSRTGRADDGPHTMKFLHNFHQETKREHDLLDVGEQSDEVVEDVEG
Sbjct: 361 EFGNGIIKWLSQVQGSRTGRADDGPHTMKFLHNFHQETKREHDLLDVGEQSDEVVEDVEG 420

Query: 482 GKGVLIKAILFLLLGTAIAAAFADPLVDVVHNFSNATNIPAFFISFIALPLATNSSEAVS 541
           GKGVLIKAILFLLLGTAIAAAFADPLVDVVHNFSNATNIPAFFISFIALPLATNSSEAVS
Sbjct: 421 GKGVLIKAILFLLLGTAIAAAFADPLVDVVHNFSNATNIPAFFISFIALPLATNSSEAVS 480

Query: 542 AIIFASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVYMRGLVWNFSSEVLVILVVTV 601
           AIIFASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVYMRGLVWNFSSEVLVILVVTV
Sbjct: 481 AIIFASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVYMRGLVWNFSSEVLVILVVTV 540

Query: 602 IMGLLGSFRTAFPLWTALVALLLYPLSLVLVYVLDYVFGWS 643
           IMGLLGSFRTAFPLWTALVALLLYPLSLVLVYVLDYVFGWS
Sbjct: 541 IMGLLGSFRTAFPLWTALVALLLYPLSLVLVYVLDYVFGWS 581

BLAST of CmaCh09G008300 vs. NCBI nr
Match: XP_023536443.1 (sodium/calcium exchanger NCL-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1080.9 bits (2794), Expect = 0.0e+00
Identity = 578/582 (99.31%), Postives = 580/582 (99.66%), Query Frame = 0

Query: 62  MSRTPLHLVFLLCFLILCVQSHSSRFASI-SSSSDLVSDGVNELQEPSYLRLNTLSSNSA 121
           MSRTPLHLVFLLCFLILCVQSHSSRFASI SSSSDLVSDGVN+LQEPSYLRLNTLSSNSA
Sbjct: 1   MSRTPLHLVFLLCFLILCVQSHSSRFASISSSSSDLVSDGVNDLQEPSYLRLNTLSSNSA 60

Query: 122 PEESCEQSYGFLPCTTSVLGNMFLIIVYGYLMFLAAKYLSAGSELLLEILGPGIVGGLFL 181
           PEESCEQSYGFLPCTTSVLGNMFLIIVYGYLMFLAAKYLSAGSELLLEILGPGIVGGLFL
Sbjct: 61  PEESCEQSYGFLPCTTSVLGNMFLIIVYGYLMFLAAKYLSAGSELLLEILGPGIVGGLFL 120

Query: 182 PALGALPDAMLILVSGLAGSAEVAQSQVSVGMGLLAGSTVMLLTLIWGTCVIVGKCDLEG 241
           PALGALPDAMLILVSGLAGSAEVAQSQVSVGMGLLAGSTVMLLTLIWGTCVIVGKCDLEG
Sbjct: 121 PALGALPDAMLILVSGLAGSAEVAQSQVSVGMGLLAGSTVMLLTLIWGTCVIVGKCDLEG 180

Query: 242 SVAIDSQDTKGFSLTESGVSTDIWTSYAARIMVISVVPFLIVQLPQMLNSTSGRHLAVLI 301
           SVAIDSQDTKGFSLTESGVSTDIWTSYAARIMVISV PFLIVQLPQMLNSTSGRHLAVLI
Sbjct: 181 SVAIDSQDTKGFSLTESGVSTDIWTSYAARIMVISVFPFLIVQLPQMLNSTSGRHLAVLI 240

Query: 302 ALIISISMFVIYCLYQVFQPWIQRRKLAFVKHKHVIFGLLRHLKQQALGRLLTENGEPDK 361
           ALIISISMF+IYCLYQVFQPWIQRRKLAFVKHKHVIFGLLRHLKQQALGRLLTENGEPDK
Sbjct: 241 ALIISISMFIIYCLYQVFQPWIQRRKLAFVKHKHVIFGLLRHLKQQALGRLLTENGEPDK 300

Query: 362 EIIDKLFSRIDENKDGRLSASELRALIVGIQFEELDLDHEDAVEKILNDFDTSRDSHVDA 421
           EIIDKLFSRIDENKDGRLSASELRALIVGIQFEELDLDHEDAVEKILNDFDTSRDSHVDA
Sbjct: 301 EIIDKLFSRIDENKDGRLSASELRALIVGIQFEELDLDHEDAVEKILNDFDTSRDSHVDA 360

Query: 422 TEFGNGIIKWLSQVQGSRTGRADDGPHTMKFLHNFHQETKREHDLLDVGEQSDEVVEDVE 481
           TEFGNGIIKWLSQVQGSRTGRADDGPHTMKFLHNFHQETKREHDLLDVGEQSDEVVEDVE
Sbjct: 361 TEFGNGIIKWLSQVQGSRTGRADDGPHTMKFLHNFHQETKREHDLLDVGEQSDEVVEDVE 420

Query: 482 GGKGVLIKAILFLLLGTAIAAAFADPLVDVVHNFSNATNIPAFFISFIALPLATNSSEAV 541
           GGKGVLIKAILFLLLGTAIAAAFADPLVDVVHNFSNATNIPAFFISFIALPLATNSSEAV
Sbjct: 421 GGKGVLIKAILFLLLGTAIAAAFADPLVDVVHNFSNATNIPAFFISFIALPLATNSSEAV 480

Query: 542 SAIIFASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVYMRGLVWNFSSEVLVILVVT 601
           SAIIFASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVYMRGLVWNFSSEVLVILVVT
Sbjct: 481 SAIIFASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVYMRGLVWNFSSEVLVILVVT 540

Query: 602 VIMGLLGSFRTAFPLWTALVALLLYPLSLVLVYVLDYVFGWS 643
           VIMGLLGSFRTAFPLWTALVALLLYPLSLVLVYVLDYVFGWS
Sbjct: 541 VIMGLLGSFRTAFPLWTALVALLLYPLSLVLVYVLDYVFGWS 582

BLAST of CmaCh09G008300 vs. NCBI nr
Match: KAG6591925.1 (Sodium/calcium exchanger NCL, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1042.3 bits (2694), Expect = 1.7e-300
Identity = 560/581 (96.39%), Postives = 563/581 (96.90%), Query Frame = 0

Query: 62  MSRTPLHLVFLLCFLILCVQSHSSRFASISSSSDLVSDGVNELQEPSYLRLNTLSSNSAP 121
           MSRTPLHLVFLLCFLILCVQSHSSRFASISSSSDLVSDGVN+LQEPSYLRLNTL SNSAP
Sbjct: 1   MSRTPLHLVFLLCFLILCVQSHSSRFASISSSSDLVSDGVNDLQEPSYLRLNTLPSNSAP 60

Query: 122 EESCEQSYGFLPCTTSVLGNMFLIIVYGYLMFLAAKYLSAGSELLLEILGPGIVGGLFLP 181
           EE+CEQSYGFLPCTTSVLGNMFLIIVYGYLMFLAAKYLSAGSELLLEILGPGIVGGLFLP
Sbjct: 61  EEACEQSYGFLPCTTSVLGNMFLIIVYGYLMFLAAKYLSAGSELLLEILGPGIVGGLFLP 120

Query: 182 ALGALPDAMLILVSGLAGSAEVAQSQVSVGMGLLAGSTVMLLTLIWGTCVIVGKCDLEGS 241
           ALGALPDAMLILVSGLAGSAEVAQSQVSVGMGLLAGSTVMLLTLIWGTCVIVGKCDLE  
Sbjct: 121 ALGALPDAMLILVSGLAGSAEVAQSQVSVGMGLLAGSTVMLLTLIWGTCVIVGKCDLE-- 180

Query: 242 VAIDSQDTKGFSLTESGVSTDIWTSYAARIMVISVVPFLIVQLPQMLNSTSGRHLAVLIA 301
                         ESGVSTDIWTSYAARIMVISV PFLIVQLPQMLNSTSGRHLAVLIA
Sbjct: 181 --------------ESGVSTDIWTSYAARIMVISVFPFLIVQLPQMLNSTSGRHLAVLIA 240

Query: 302 LIISISMFVIYCLYQVFQPWIQRRKLAFVKHKHVIFGLLRHLKQQALGRLLTENGEPDKE 361
           LIISISMF+IYCLYQVFQPWIQRRKLAFVKHKHVIFGLLRHLKQQALGRLLTENGEPDKE
Sbjct: 241 LIISISMFIIYCLYQVFQPWIQRRKLAFVKHKHVIFGLLRHLKQQALGRLLTENGEPDKE 300

Query: 362 IIDKLFSRIDENKDGRLSASELRALIVGIQFEELDLDHEDAVEKILNDFDTSRDSHVDAT 421
           IIDKLFSRIDENKDGRLSASELRALIVGIQFEELDLDHEDAVEKILNDFDTSRDSHVDAT
Sbjct: 301 IIDKLFSRIDENKDGRLSASELRALIVGIQFEELDLDHEDAVEKILNDFDTSRDSHVDAT 360

Query: 422 EFGNGIIKWLSQVQGSRTGRADDGPHTMKFLHNFHQETKREHDLLDVGEQSDEVVEDVEG 481
           EFGNGIIKWLSQVQGSRTGRADDGPHTMKFLHNFHQETKREHDLLDVGEQSDEVVEDVEG
Sbjct: 361 EFGNGIIKWLSQVQGSRTGRADDGPHTMKFLHNFHQETKREHDLLDVGEQSDEVVEDVEG 420

Query: 482 GKGVLIKAILFLLLGTAIAAAFADPLVDVVHNFSNATNIPAFFISFIALPLATNSSEAVS 541
           GKGVLIKAILFLLLGTAIAAAFADPLVDVVHNFSNATNIPAFFISFIALPLATNSSEAVS
Sbjct: 421 GKGVLIKAILFLLLGTAIAAAFADPLVDVVHNFSNATNIPAFFISFIALPLATNSSEAVS 480

Query: 542 AIIFASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVYMRGLVWNFSSEVLVILVVTV 601
           AIIFASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVYMRGLVWNFSSEVLVILVVTV
Sbjct: 481 AIIFASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVYMRGLVWNFSSEVLVILVVTV 540

Query: 602 IMGLLGSFRTAFPLWTALVALLLYPLSLVLVYVLDYVFGWS 643
           IMGLLGSFRTAFPLWTALVALLLYPLSLVLVYVLDYVFGWS
Sbjct: 541 IMGLLGSFRTAFPLWTALVALLLYPLSLVLVYVLDYVFGWS 565

BLAST of CmaCh09G008300 vs. NCBI nr
Match: XP_023523899.1 (sodium/calcium exchanger NCL-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1010.4 bits (2611), Expect = 7.1e-291
Identity = 536/581 (92.25%), Postives = 560/581 (96.39%), Query Frame = 0

Query: 62  MSRTPLHLVFLLCFLILCVQSHSSRFASISSSSDLVSDGVNELQEPSYLRLNTLSSNSAP 121
           MSR PL+L+FLL F + C QSHSSRFASISSSSDLVSDGVNELQE  YLRLN+L S SAP
Sbjct: 1   MSRPPLYLIFLLIFFLFCGQSHSSRFASISSSSDLVSDGVNELQETPYLRLNSLESVSAP 60

Query: 122 EESCEQSYGFLPCTTSVLGNMFLIIVYGYLMFLAAKYLSAGSELLLEILGPGIVGGLFLP 181
           EESCE+SYGFLPCTTSVLGN+FLIIVYGYLMFLAAKYLS GSELLLEILGPGIVGGLFLP
Sbjct: 61  EESCEESYGFLPCTTSVLGNLFLIIVYGYLMFLAAKYLSTGSELLLEILGPGIVGGLFLP 120

Query: 182 ALGALPDAMLILVSGLAGSAEVAQSQVSVGMGLLAGSTVMLLTLIWGTCVIVGKCDLEGS 241
           ALGALPDAMLILVSGLAGSAEVAQSQVSVGMGLLAGSTVMLLTLIWGTCVIVGKCDL+ S
Sbjct: 121 ALGALPDAMLILVSGLAGSAEVAQSQVSVGMGLLAGSTVMLLTLIWGTCVIVGKCDLQDS 180

Query: 242 VAIDSQDTKGFSLTESGVSTDIWTSYAARIMVISVVPFLIVQLPQMLNSTSGRHLAVLIA 301
           VAID QDTKGFSLTESGVSTDIWTSYAARIMVISVVPFL+VQLPQ+LNSTSGRHLAVLIA
Sbjct: 181 VAIDLQDTKGFSLTESGVSTDIWTSYAARIMVISVVPFLVVQLPQLLNSTSGRHLAVLIA 240

Query: 302 LIISISMFVIYCLYQVFQPWIQRRKLAFVKHKHVIFGLLRHLKQQALGRLLTENGEPDKE 361
           LIIS+SMF+IYCLYQVFQPWIQRRKLAFVKHKHVIFGLLRHLKQQALGRLLTE+GEPDKE
Sbjct: 241 LIISVSMFIIYCLYQVFQPWIQRRKLAFVKHKHVIFGLLRHLKQQALGRLLTESGEPDKE 300

Query: 362 IIDKLFSRIDENKDGRLSASELRALIVGIQFEELDLDHEDAVEKILNDFDTSRDSHVDAT 421
           IIDKLFSRID NK+G LSASELRALIVGIQFE +DLDH+DAV KIL+DFDTSRDSHVDAT
Sbjct: 301 IIDKLFSRIDANKNGLLSASELRALIVGIQFEAIDLDHDDAVNKILSDFDTSRDSHVDAT 360

Query: 422 EFGNGIIKWLSQVQGSRTGRADDGPHTMKFLHNFHQETKREHDLLDVGEQSDEVVEDVEG 481
           EF NGII+WL+ VQG+RTGRADDGPHTMK+LHNFHQETKREHDLLDVGEQSDEVVE VE 
Sbjct: 361 EFANGIIRWLAHVQGARTGRADDGPHTMKYLHNFHQETKREHDLLDVGEQSDEVVEGVEE 420

Query: 482 GKGVLIKAILFLLLGTAIAAAFADPLVDVVHNFSNATNIPAFFISFIALPLATNSSEAVS 541
            KGVLI+AILFLLLGTAIAAAFADPLVDVVHNFSNAT+IPAFFISFIALPLATNSSEAVS
Sbjct: 421 SKGVLIRAILFLLLGTAIAAAFADPLVDVVHNFSNATSIPAFFISFIALPLATNSSEAVS 480

Query: 542 AIIFASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVYMRGLVWNFSSEVLVILVVTV 601
           AIIFASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVY+RGLVWNFSSEVLVILVVT+
Sbjct: 481 AIIFASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVYLRGLVWNFSSEVLVILVVTM 540

Query: 602 IMGLLGSFRTAFPLWTALVALLLYPLSLVLVYVLDYVFGWS 643
           IMGLLGSFRTAFPLWTALVALLLYPLSLVLVYVLDYVFGWS
Sbjct: 541 IMGLLGSFRTAFPLWTALVALLLYPLSLVLVYVLDYVFGWS 581

BLAST of CmaCh09G008300 vs. TAIR 10
Match: AT1G53210.1 (sodium/calcium exchanger family protein / calcium-binding EF hand family protein )

HSP 1 Score: 712.6 bits (1838), Expect = 2.8e-205
Identity = 386/577 (66.90%), Postives = 453/577 (78.51%), Query Frame = 0

Query: 73  LCFLILCVQSHSSRFASIS-SSSDLVSDGV----NELQEPSYLRLNTLSSNSAPEESCEQ 132
           L FL+    S  +RF S++ SS+ L+SDG+    N     S ++    +     EE+CEQ
Sbjct: 9   LLFLLFFTSSAYARFVSLNPSSTSLISDGIDGGSNLAGSGSVIKSVVSAPAEEKEEACEQ 68

Query: 133 SYGFLPCTTSVLGNMFLIIVYGYLMFLAAKYLSAGSELLLEILGPGIVGGLFLPALGALP 192
           +YGF+PCT + LGN+FLI+VYG+LMF AA YLSAGSELLLEILGPGIVGGLFLP LGALP
Sbjct: 69  TYGFMPCTKTALGNVFLILVYGFLMFTAATYLSAGSELLLEILGPGIVGGLFLPMLGALP 128

Query: 193 DAMLILVSGLAGSAEVAQSQVSVGMGLLAGSTVMLLTLIWGTCVIVGKCDLEGSVAIDSQ 252
           DAMLI+VSGL+G A  AQSQVSVGMGLLAGSTVMLLT+IWGTC +VGKCDL  S+A+++Q
Sbjct: 129 DAMLIMVSGLSGDAATAQSQVSVGMGLLAGSTVMLLTVIWGTCTVVGKCDLRDSIAVNNQ 188

Query: 253 DTKGFSLTESGVSTDIWTSYAARIMVISVVPFLIVQLPQMLNSTSGRHLAVLIALIISIS 312
           DTKGF L +SGV+ DIWTSYAARIM ISV+PF+IVQLPQML STSGR L+VLIALI+S+ 
Sbjct: 189 DTKGFHLKDSGVTVDIWTSYAARIMAISVIPFVIVQLPQMLGSTSGRQLSVLIALILSVL 248

Query: 313 MFVIYCLYQVFQPWIQRRKLAFVKHKHVIFGLLRHLKQQALGRLLTENGEPDKEIIDKLF 372
           M + YC+YQVFQPWIQRR+LAF KHKHVI G+LRHLKQ ALGRLL + G+PD+ +I KLF
Sbjct: 249 MLISYCVYQVFQPWIQRRRLAFAKHKHVISGILRHLKQHALGRLLDDEGQPDEHVIRKLF 308

Query: 373 SRIDENKDGRLSASELRALIVGIQFEELDLDHEDAVEKILNDFDTSRDSHVDATEFGNGI 432
             ID N DG LSA+EL+ALI+GI FE++D D +DAV K+L DFD + D  VD  EF  GI
Sbjct: 309 LTIDANNDGHLSAAELKALIIGISFEDIDFDKDDAVGKVLQDFDKTLDEQVDQEEFVRGI 368

Query: 433 IKWLSQVQGSRTGRADDGPHTMKFLHNFHQETKREHDLLDVGE--QSDEVVEDVEGGKGV 492
            +WL Q  G      + GP TMKFL NFH +TKREH LL   E  ++DE   +V   K +
Sbjct: 369 KQWLIQAMGGAPSGPEAGPRTMKFLDNFHVQTKREHALLGDNENGENDEEGGEVADPKWI 428

Query: 493 LIKAILFLLLGTAIAAAFADPLVDVVHNFSNATNIPAFFISFIALPLATNSSEAVSAIIF 552
            IKA L LLLG AIAAAFADPLVD V+NFS AT IP+FFISFIALPLATNSSEAVSAIIF
Sbjct: 429 TIKAALLLLLGAAIAAAFADPLVDTVNNFSAATGIPSFFISFIALPLATNSSEAVSAIIF 488

Query: 553 ASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVYMRGLVWNFSSEVLVILVVTVIMGL 612
           ASR K +TASLTFSEL G VTMNN+LCLSVFLA+VY+RGL WNFSSEVLVIL+V ++MG 
Sbjct: 489 ASRKKIRTASLTFSELCGGVTMNNILCLSVFLAIVYVRGLTWNFSSEVLVILIVCLVMGG 548

Query: 613 LGSFRTAFPLWTALVALLLYPLSLVLVYVLDYVFGWS 643
             SFRT +PLWT  +A LLYP SL LVY+LDY FGWS
Sbjct: 549 FASFRTTYPLWTCFIAYLLYPFSLGLVYILDYWFGWS 585

BLAST of CmaCh09G008300 vs. TAIR 10
Match: AT1G29025.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 247.7 bits (631), Expect = 2.6e-65
Identity = 195/603 (32.34%), Postives = 303/603 (50.25%), Query Frame = 0

Query: 62  MSRTPLHLVFLLCFLILCVQSH-SSRFASISSSSDLVSDGVNELQEPSYLRLNTLSSNSA 121
           M+R  L L FL   L+  V     S  +  ++SS LVSDGV +     +L LN     + 
Sbjct: 1   MARLTLFLSFLSLALVSVVNCRVLSSVSEDNNSSILVSDGVQDRSSNDFLSLN---PPNL 60

Query: 122 PEESCEQSYGFLPCTTSVLGNMFLIIVYGYLMFLAAKYLSAGSELLLEILGPGIVGGLFL 181
            E +C   YGFLPC  ++ G +F +  +G L+ +   +LS G   L  I   G  GG+  
Sbjct: 61  SESACVHVYGFLPCADNIEGYVFQVFSFGCLLIIGEYFLSKGRSKLFVIFEVGFFGGIIF 120

Query: 182 PALGALPDAMLILVSGLAGSAEVAQSQVSVGMGLLAGSTVMLLTLIWGTCVIVGKCDL-- 241
           P L   P   LIL +GL GS E+A S     + +  G +V  LT+ WG CV+ G   L  
Sbjct: 121 PLLTMFPRIALILSTGLIGSREIANSMTGNNVAVTVGYSVFALTMQWGACVVFGLSGLRS 180

Query: 242 -EGSVAID-----SQDTKGFSLTE----SGVSTDIWTSYAARIMVISVVPFLIVQLPQML 301
            + S+  D      Q  K  +L +    + +  D     AA IM+++++PF++V   +  
Sbjct: 181 DDQSITCDISSPRRQVKKSINLLKNLAGASIRADPKNKKAAGIMLLTLLPFVLVTFSETF 240

Query: 302 NSTSGRHLAVLIALIISISMFVIYCLYQVFQPWIQRRKLAFVK-------HKHVIFGLLR 361
           +  S     VL+ LI S S  V+Y +Y  F    Q + L + K       HKH     L+
Sbjct: 241 HIKSWDDNMVLVTLIFSGSATVLYFVYLYFDKADQVKSLEYAKFELMSEVHKH-----LQ 300

Query: 362 HLKQQALGRLLTENGEPDKEIIDKLFSRIDENKDGRLSASELRALIVGI-QFEELDLDHE 421
           +   Q+L R    NG+  +E +  LF + D NKDG++  SEL+ L V       +  D  
Sbjct: 301 NFSPQSLIR----NGQLCQESLKSLFKKFDVNKDGKIQVSELKDLTVDFGVLGRVKCDIN 360

Query: 422 DAVEKILNDFDTSRDSHVDATEFGNGIIKWLSQVQGSRTGRADDGPHTMKFLHNFHQETK 481
           +    +L DFD++RD  +D TEF  GI KWL Q + S  G    G               
Sbjct: 361 ELATTLLADFDSNRDGEIDETEFAIGIEKWLKQYKFSFNGTESQGE-------------- 420

Query: 482 REHDLLDVGEQSDEVVEDVEGGKGVL---------IKAILFLLLGTAIAAAFADPLVDVV 541
                 D+ E  D V++  +  KG L         +KA++ +++G  I +  A P +  +
Sbjct: 421 ------DIAE-DDGVLKVEQLLKGCLFTKLLTKRTLKAVIEVIIGITIVSFLAMPFMMNI 480

Query: 542 HNFSNATNIPAFFISFIALPLATNSSEAVSAIIFASRDKRKTASLTFSELYGAVTMNNVL 601
              S +  +P+F++ F  +PLA N   A+SA     ++K K  S TFSE+Y  VT+NN+L
Sbjct: 481 ELLSVSAGVPSFYVVFAVIPLARNLKNALSAHFCRKKEKAKITSDTFSEIYRDVTLNNLL 540

Query: 602 CLSVFLALVYMRGLVWNFSSEVLVILVVTVIMGLLGSFRTAFPLWTALVALLLYPLSLVL 635
            +++ LA+VY+RGL W++S+EVL+I++V +I+G+    R+ +P W  ++A  LY  SL+L
Sbjct: 541 GITIILAIVYIRGLTWDYSTEVLIIVIVGLIIGVPAYVRSTYPFWICVLAFALYFFSLIL 570

BLAST of CmaCh09G008300 vs. TAIR 10
Match: AT1G29020.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 237.3 bits (604), Expect = 3.5e-62
Identity = 180/580 (31.03%), Postives = 294/580 (50.69%), Query Frame = 0

Query: 62  MSRTPLHLVFLLCFLILCVQSHSSRFASISSSSDLVSDGVNELQEPSYLRLNTLSSNSAP 121
           M+R  L L FL    ++ V +     +     S LVSDGV +     +L L+    N   
Sbjct: 1   MARLSLFLSFLSLIALISVVNCRVLSSLSEKDSILVSDGVQDGSSYEFLSLD--PPNDIT 60

Query: 122 EESCEQSYGFLPCTTSVLGNMFLIIVYGYLMFLAAKYLSAGSELLLEILGPGIVGGLFLP 181
           +  C   Y FLPC  +V G +F +  +G ++ +   +L+ G   L  I   G  GG+  P
Sbjct: 61  KNMCVHVYDFLPCADNVAGYVFQVFSFGCILIIGEYFLTKGRTKLFLIFEVGFYGGIIFP 120

Query: 182 ALGALPDAMLILVSGLAGSAEVAQSQVSVGMGLLAGSTVMLLTLIWGTCVIVGKCDLEGS 241
            L   P   LIL +GL GS E+A S+V   +G+  G +V  LT+ WG C++ G    +  
Sbjct: 121 LLTMFPRIALILSTGLVGSREMASSRVGNIIGVTVGYSVFALTIQWGACILFGLSGTKPE 180

Query: 242 VAIDSQDTKGFSLTESGVSTDIWTSYAARIMVISVVPFLIVQLPQMLNSTSGRHLAVLIA 301
             ID        L+ + V  DI    +A IM+++++PF++V   ++ +S     + VLI 
Sbjct: 181 QPID--------LSAASVEADITNKKSAGIMLLTILPFILVTFSEISSSQYWNDIIVLIT 240

Query: 302 LIISISMFVIYCLYQVFQPWIQRRKLAFVKHKHVIFGLLRHLKQQALGRLLTENGEPDKE 361
           LIIS S    Y  Y  F    Q + L   + + ++  + +HL Q    + L  +G+ ++E
Sbjct: 241 LIISASATFAYFFYSYFDRDDQAKSLDHARFE-LMSEVHKHL-QIFSPKSLIRDGQLNQE 300

Query: 362 IIDKLFSRIDENKDGRLSASELRALIVGI-QFEELDLDHEDAVEKILNDFDTSRDSHVDA 421
            + +LF +ID N DG++  +EL+ L V       +    +  V  +++DFD   D  +D 
Sbjct: 301 SLKRLFQKIDANGDGKIQVAELKDLTVEFGMIGRVKCHIDKLVHTLVDDFDRDGDREIDE 360

Query: 422 TEFGNGIIKWLSQVQGS-------RTGRADDGPHTMKFLHNFHQETKREHDLLDVGEQSD 481
            EF  GI KWL+Q + S       R  +A+  P                  +L V +   
Sbjct: 361 AEFEIGIKKWLNQYKFSFDTTVPPREDQAEGVP------------------ILKVEKPEQ 420

Query: 482 EVVEDVEGGKGVLIKAILFLLLGTAIAAAFADPLVDVVHNFSNATNIPAFFISFIALPLA 541
            +V+ +   +   ++A L ++LG  +    A P +  +   S +  +P+F++ F+ +PLA
Sbjct: 421 SLVKKLLSWR--TLRASLEVILGMLVVIYLARPFMMNIQLLSESAGVPSFYVVFVVIPLA 480

Query: 542 TNSSEAVSAIIFASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVYMRGLVWNFSSEV 601
            N    +SA     +DK K  S TFSE+Y  VTMNN++ +S+ LA+VY R LVW++++EV
Sbjct: 481 RNLKNTLSAHFCRKKDKAKITSDTFSEIYKDVTMNNLMGISIILAIVYARELVWDYTTEV 540

Query: 602 LVILVVTVIMGLLGSFRTAFPLWTALVALLLYPLSLVLVY 634
           L+I++V +++GL    RT +P W  L+A  LY +SLV+VY
Sbjct: 541 LIIVIVGLLIGLPAYVRTTYPFWICLLAFALYFISLVVVY 548

BLAST of CmaCh09G008300 vs. TAIR 10
Match: AT2G34020.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 234.6 bits (597), Expect = 2.3e-61
Identity = 196/620 (31.61%), Postives = 302/620 (48.71%), Query Frame = 0

Query: 63  SRTPLHLVFLLCFLILCVQSHSSRFAS--ISSSSDLVSDGVNELQEPSYLRLNTLSSNSA 122
           S  P  L F    L L +   SSR  S     +S L+SDGV+   +  YL L+     + 
Sbjct: 13  SHFPSTLFFFFLSLTLFISGVSSRVLSPVPLDNSSLISDGVHGASDYKYLTLD--PPKNV 72

Query: 123 PEESCEQSYGFLPCTTSVLGNMFLIIVYGYLMFLAAKYLSAGSELLLEILGPGIVGGLFL 182
            + +C   YGFLPC  ++ G +F +  +G L+ +   +LS G   L  I   G  GG+  
Sbjct: 73  SKAACIHVYGFLPCADNIGGYVFQVFSFGCLLIIGDYFLSQGRSKLFVIFEVGFYGGIVF 132

Query: 183 PALGALPDAMLILVSGLAGSAEVAQSQVSVGMGLLAGSTVMLLTLIWGTCVIVGKCD--- 242
           P L   P   L++  GLA + E A   V   +G+  G T+  LT+ WG CVI G      
Sbjct: 133 PLLTMFPRIALMISPGLAATHEGALMIVGNNVGVTIGHTIFALTMQWGACVIFGLTSPNS 192

Query: 243 ----LEGSVAIDSQDTK----GF-------SLTESGVSTDIWTSYAARIMVISVVPFLIV 302
                 GS+   S DTK    GF       S+ E+ V  D     AA IM++++VPFL+V
Sbjct: 193 DPSIRRGSIKRTSSDTKNPRRGFYRMKILKSVVEASVDADPKNKKAAGIMLLTLVPFLLV 252

Query: 303 QLPQMLNSTSGRHLAVLIALIISISMFVIYCLYQVFQPWIQRRKLAFVKHKHVIFGLLRH 362
            LP +L+  S   + +LI LIIS S   IY +Y  F    Q++ L   K + ++  + +H
Sbjct: 253 TLPDLLDVQSWSDIIMLITLIISCSSTFIYFVYSYFDTADQKKSLDHAKFE-LMSEVHKH 312

Query: 363 LKQQALGRLLTENGEPDKEIIDKLFSRIDENKDGRLSASELRALIVGI-QFEELDLDHED 422
           L Q    R L  +G+  KE +  LF +ID NKDG++  SEL+ L V    F ++  D  +
Sbjct: 313 L-QSFSPRTLIRDGQLSKESLKSLFDKIDRNKDGKIQISELKDLTVEFGVFGKMKCDINE 372

Query: 423 AVEKILNDFDTSRDSHVDATEFGNGIIKWLSQVQGSRTGRADDGPHTMKFLHNFHQETKR 482
               +L +FD  ++  +D  EF  GI+K L+  +                    +QE+ R
Sbjct: 373 FASTLLAEFDKDKNGELDENEFEEGIMKLLNHYKFD------------------NQESPR 432

Query: 483 EHDLLDVGEQSDEVVEDVEGGK-GVL------------------IKAILFLLLGTAIAAA 542
           +++       SD V    +G + GVL                  ++A+  ++ G  I   
Sbjct: 433 QNNTYIYRTPSDSVKSLSQGEEAGVLKLEMPKQTLVAKFLSMSTLRAVTKVIGGMLIVVF 492

Query: 543 FADPLVDVVHNFSNATNIPAFFISFIALPLATNSSEAVSAIIFASRDKRKTASLTFSELY 602
            A P +  +   S +  +P+F+  F  +PL  N    +SA     +DK + AS  FSE+Y
Sbjct: 493 LAKPFMVNIGLLSVSAGVPSFYSVFAVIPLVRNLKNTLSAHFCRKKDKARIASEKFSEIY 552

Query: 603 GAVTMNNVLCLSVFLALVYMRGLVWNFSSEVLVILVVTVIMGLLGSFRTAFPLWTALVAL 643
             VTMNN++ +S+ LA+VY RGL W +S E L+++VV + +GL    R+ +P W  ++A 
Sbjct: 553 RDVTMNNLMGMSITLAIVYSRGLKWEYSIESLLVVVVGIAIGLPAYVRSTYPFWICVMAF 610

BLAST of CmaCh09G008300 vs. TAIR 10
Match: AT2G34030.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 231.1 bits (588), Expect = 2.5e-60
Identity = 183/582 (31.44%), Postives = 295/582 (50.69%), Query Frame = 0

Query: 69  LVFLLCFLILCVQSHSSRFASIS---SSSDLVSDGVNELQEPSYLRLNTLSSNSAPEESC 128
           L F L  + L +   SSR        ++S L+SDG+++     +L L+   S S    +C
Sbjct: 6   LFFSLLSIALIISGVSSRVLISHVPLNNSILISDGIHDALNHEFLTLDPPKSLS--RTAC 65

Query: 129 EQSYGFLPCTTSVLGNMFLIIVYGYLMFLAAKYLSAGSELLLEILGPGIVGGLFLPALGA 188
              YGFLPC  +V G +F +  +G L+ +   +LS G   L  I   G  GG+  P L  
Sbjct: 66  VHVYGFLPCADNVEGYIFQVFSFGSLLIIGDYFLSEGRSKLFVIFEVGFYGGIIFPLLTM 125

Query: 189 LPDAMLILVSGLAGSAEVAQSQVSVGMGLLAGSTVMLLTLIWGTCVIVG----KCDLEGS 248
            P   L+L +GL+ S +VA S +   +GL  G TV  LT+ WG CV+      + D    
Sbjct: 126 FPRIALMLSTGLSLSRDVASSFIDDNVGLTVGHTVFSLTIQWGACVVFSITGPRSDQADG 185

Query: 249 VAIDSQDTKGFSLTESGVSTDIWTSYAARIMVISVVPFLIVQLPQMLNSTSGRHLAVLIA 308
           +    +  KGF   E+ V  D     AA IM++S+ PFL+V    + +S S  H+ VLI 
Sbjct: 186 LIEKLKILKGF--VEARVEADPKNKKAAGIMLLSLSPFLMVTFSAIFDSHSWSHIIVLIT 245

Query: 309 LIISISMFVIYCLYQVFQPWIQRRKLAFVKHKHVIFGLLRHLKQQALGRLLTENGEPDKE 368
           LIIS S  V+Y +Y       Q + L   + + ++  + +HLK+ +   L+ ++GE  KE
Sbjct: 246 LIISSSSTVVYFVYSYLDTANQEKSLDHARFE-LMSEVHKHLKRFSPKHLI-KDGELSKE 305

Query: 369 IIDKLFSRIDENKDGRLSASELRALIVGI-QFEELDLDHEDAVEKILNDFDTSRDSHVDA 428
            +  LF + D+NKDG++  SEL+ L + +  F  +  D  +  +  L DFD   D  ++ 
Sbjct: 306 SLKSLFKKTDKNKDGKIQISELKDLTIELSNFGRMRYDINELAKAFLEDFDGDNDGELEE 365

Query: 429 TEFGNGIIKWLSQVQGSRTGRADDGPHTMKFLHNFHQETKREHDLLDVGEQSDEVVEDVE 488
            EF  GI + L Q                   + F+ E +RE+      E++  +  +++
Sbjct: 366 NEFEEGIARLLKQ-------------------YKFNVEDQRENQT----EENGVLKLEIK 425

Query: 489 GGKGVLIK--------AILFLLLGTAIAAAFADPLVDVVHNFSNATNIPAFFISFIALPL 548
             K ++ K        A   +++G  I    A P +  +   S +  IP+F+I F  +P 
Sbjct: 426 PKKTLVTKLLSMETLIATTEVIVGILIVLFLAKPFMLNIQLLSISAGIPSFYIVFAMIPF 485

Query: 549 ATNSSEAVSAIIFASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVYMRGLVWNFSSE 608
           A N    +S      +DK++ +S TFSE+Y  +TMNN+L +SV LA+VY RGL W +S E
Sbjct: 486 ARNLKNTLSTRFCRGKDKKRVSSNTFSEIYKDITMNNLLGMSVILAIVYTRGLTWKYSVE 545

Query: 609 VLVILVVTVIMGLLGSFRTAFPLWTALVALLLYPLSLVLVYV 635
            L++++V +I+GL    R+ +P W  ++A  +Y  SL+L+Y+
Sbjct: 546 TLIMVIVGLIIGLPIYIRSTYPFWMCVLAFAMYFFSLLLIYI 558

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8L6364.0e-20466.90Sodium/calcium exchanger NCL OS=Arabidopsis thaliana OX=3702 GN=NCL PE=2 SV=1[more]
Q5QNI24.0e-18861.86Sodium/calcium exchanger NCL1 OS=Oryza sativa subsp. japonica OX=39947 GN=NCL1 P... [more]
Q6K3R52.9e-17859.21Sodium/calcium exchanger NCL2 OS=Oryza sativa subsp. japonica OX=39947 GN=NCL2 P... [more]
Q5KQN01.1e-0430.94Vacuolar cation/proton exchanger 2 OS=Oryza sativa subsp. japonica OX=39947 GN=C... [more]
Match NameE-valueIdentityDescription
A0A6J1IGZ10.0e+00100.00sodium/calcium exchanger NCL-like OS=Cucurbita maxima OX=3661 GN=LOC111476856 PE... [more]
A0A6J1FAG10.0e+0099.14sodium/calcium exchanger NCL-like OS=Cucurbita moschata OX=3662 GN=LOC111443554 ... [more]
A0A6J1FT503.8e-29091.74sodium/calcium exchanger NCL-like OS=Cucurbita moschata OX=3662 GN=LOC111446626 ... [more]
A0A5A7VFE48.4e-29090.85Sodium/calcium exchanger family protein OS=Cucumis melo var. makuwa OX=1194695 G... [more]
A0A1S3CJX38.4e-29090.85uncharacterized protein LOC103501799 OS=Cucumis melo OX=3656 GN=LOC103501799 PE=... [more]
Match NameE-valueIdentityDescription
XP_022976461.10.0e+00100.00sodium/calcium exchanger NCL-like [Cucurbita maxima][more]
XP_022937179.10.0e+0099.14sodium/calcium exchanger NCL-like [Cucurbita moschata] >KAG7024799.1 Sodium/calc... [more]
XP_023536443.10.0e+0099.31sodium/calcium exchanger NCL-like [Cucurbita pepo subsp. pepo][more]
KAG6591925.11.7e-30096.39Sodium/calcium exchanger NCL, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_023523899.17.1e-29192.25sodium/calcium exchanger NCL-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
AT1G53210.12.8e-20566.90sodium/calcium exchanger family protein / calcium-binding EF hand family protein... [more]
AT1G29025.12.6e-6532.34Calcium-binding EF-hand family protein [more]
AT1G29020.13.5e-6231.03Calcium-binding EF-hand family protein [more]
AT2G34020.12.3e-6131.61Calcium-binding EF-hand family protein [more]
AT2G34030.12.5e-6031.44Calcium-binding EF-hand family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002048EF-hand domainSMARTSM00054efh_1coord: 402..430
e-value: 33.0
score: 5.5
coord: 362..390
e-value: 0.011
score: 24.9
IPR002048EF-hand domainPFAMPF13499EF-hand_7coord: 362..423
e-value: 3.3E-7
score: 30.7
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 358..393
score: 12.78389
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 398..433
score: 8.766953
IPR002048EF-hand domainCDDcd00051EFhcoord: 363..423
e-value: 8.61104E-7
score: 44.4609
NoneNo IPR availableGENE3D1.10.238.10coord: 339..464
e-value: 3.1E-10
score: 41.8
NoneNo IPR availablePANTHERPTHR31503:SF46SUBFAMILY NOT NAMEDcoord: 70..640
IPR044880NCX, central ion-binding domain superfamilyGENE3D1.20.1420.30coord: 170..338
e-value: 3.2E-5
score: 25.4
IPR004837Sodium/calcium exchanger membrane regionPFAMPF01699Na_Ca_excoord: 490..633
e-value: 1.1E-11
score: 44.9
coord: 143..319
e-value: 4.1E-7
score: 30.1
IPR004713Calcium/proton exchangerPANTHERPTHR31503VACUOLAR CALCIUM ION TRANSPORTERcoord: 70..640
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 371..383
IPR011992EF-hand domain pairSUPERFAMILY47473EF-handcoord: 344..567

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh09G008300.1CmaCh09G008300.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0070588 calcium ion transmembrane transport
biological_process GO:0006874 cellular calcium ion homeostasis
biological_process GO:0006812 cation transport
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005774 vacuolar membrane
molecular_function GO:0005509 calcium ion binding
molecular_function GO:0015369 calcium:proton antiporter activity
molecular_function GO:0008324 cation transmembrane transporter activity