CmaCh09G005320 (gene) Cucurbita maxima (Rimu) v1.1

Overview


Name	CmaCh09G005320
Type	gene
Organism	Cucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Description	Sarcosine oxidase
Location	Cma_Chr09: 2374439 .. 2375836 (-)
RNA-Seq Expression	CmaCh09G005320
Synteny	CmaCh09G005320

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGTATTTTCGTCATTTCATTAAAACATGAGCTTCCTACACGAAGGTGCAGTAGCACACTTCAATTCAACTTCAATAACTCTCTTCCATTACTCTACACGCAAATTCCCCTCATCGGAGACTCGGAGGAGAGGAAAAAAAGTTCCAAACAAATTGGAGGAGAAGAAATGGCCGATTCCGGCACTCAATATGATGTAATCGTCGTCGGCGCCGGCGTAATGGGCAGTTCGACGGCATACCATCTCGCTAAAACAGGGAACAAAGTTCTGATTCTGGAGCAGTTCGATTTTCTTCACCATAGAGGCTCTTCTCATGGAGAATCTCGCACCATACGCGCCACCTATCCGGAGGATTATTATCACAGTCTCGTTATGGAATCTTACGAGCTCTGGCGGACGGCGGAGGCAGAAATTGGTTACAGGGTATATTTTCCGGCGGAGCAGCTCGATATCGGCCATTCCGACGATAAAAGTCTCGCCGCCGCCGTCGATACCTGCAAGAAGCATTCGATCCCGCATCTGGTTCTGGATCGGGAGCAACTGGCGGAGAAGTACTCTGGCAGGGTGGAGATCCCGGCGGACTGGGTGGCGGTGTGGAGCAAGTACGGTGGCGTAATTAAGCCGACGAAGGCGGTTTCGATGTTCCAAACTCTGGCGTTCAAAAACGGCGCTGATTTGAAGGACAACGCGGAAGTGGTGGAGATAAAGAGAGATGGGAGTAGTGGAGGAGTAGTTGTTTCAGTAGCCAATGGGGAGAGATTTAGGGGAAAGAAATGTGTGGTGACAGTTGGAGCTTGGGCTAGAAAGTTAGTTAAATCAGTTGGTGGGATTGAATTGCCAATTCAACCATTGGAGGCTACTGTGTCCTACTGGAGGATCAAGGAGGGAGCTGAGGGGGAGTATGCAATCGGCGGGAACTTTCCGACGTTTGCTAGCTACGGTGACACATATATCTACGGAACGCCATCGTTGGAGTTTCCGGGATTGATTAAGGTAGCCGTGCATGGCGGGCATCGGTGCGACCCGGACAAACGGTCATGGGGGAGTGGGGGGCAGCTGCCGATAACTGCATTAAAGAAGTGGATAGAGGGGAGGTTTGGGGGGAGGGTGGATTCAAGTGAGCCAGTGTCGACACAACTGTGTATGTACTCAATGACGCCAGATGAAGACTTTGTGATTGATTTTTTGGGAGGAGAATTTGGGAAGGATGTGGTAATCGGCGGTGGGTTTTCGGGGCACGGGTTCAAAATGTCGCCAGCGATCGGGAGGATACTGGCAGATCTTGCATTGAAGGGGGTGGCGGAGGGGTTGGAGCTGAAGTATTTCAGGATAGCAAGGTTTGAGGAGAATCCAAAAGGTAATGTCAAGGGCTTTGCAGATCAAGTAAGGCTTCACTAG

mRNA sequence

ATGGGTATTTTCGTCATTTCATTAAAACATGAGCTTCCTACACGAAGGTGCAGTAGCACACTTCAATTCAACTTCAATAACTCTCTTCCATTACTCTACACGCAAATTCCCCTCATCGGAGACTCGGAGGAGAGGAAAAAAAGTTCCAAACAAATTGGAGGAGAAGAAATGGCCGATTCCGGCACTCAATATGATGTAATCGTCGTCGGCGCCGGCGTAATGGGCAGTTCGACGGCATACCATCTCGCTAAAACAGGGAACAAAGTTCTGATTCTGGAGCAGTTCGATTTTCTTCACCATAGAGGCTCTTCTCATGGAGAATCTCGCACCATACGCGCCACCTATCCGGAGGATTATTATCACAGTCTCGTTATGGAATCTTACGAGCTCTGGCGGACGGCGGAGGCAGAAATTGGTTACAGGGTATATTTTCCGGCGGAGCAGCTCGATATCGGCCATTCCGACGATAAAAGTCTCGCCGCCGCCGTCGATACCTGCAAGAAGCATTCGATCCCGCATCTGGTTCTGGATCGGGAGCAACTGGCGGAGAAGTACTCTGGCAGGGTGGAGATCCCGGCGGACTGGGTGGCGGTGTGGAGCAAGTACGGTGGCGTAATTAAGCCGACGAAGGCGGTTTCGATGTTCCAAACTCTGGCGTTCAAAAACGGCGCTGATTTGAAGGACAACGCGGAAGTGGTGGAGATAAAGAGAGATGGGAGTAGTGGAGGAGTAGTTGTTTCAGTAGCCAATGGGGAGAGATTTAGGGGAAAGAAATGTGTGGTGACAGTTGGAGCTTGGGCTAGAAAGTTAGTTAAATCAGTTGGTGGGATTGAATTGCCAATTCAACCATTGGAGGCTACTGTGTCCTACTGGAGGATCAAGGAGGGAGCTGAGGGGGAGTATGCAATCGGCGGGAACTTTCCGACGTTTGCTAGCTACGGTGACACATATATCTACGGAACGCCATCGTTGGAGTTTCCGGGATTGATTAAGGTAGCCGTGCATGGCGGGCATCGGTGCGACCCGGACAAACGGTCATGGGGGAGTGGGGGGCAGCTGCCGATAACTGCATTAAAGAAGTGGATAGAGGGGAGGTTTGGGGGGAGGGTGGATTCAAGTGAGCCAGTGTCGACACAACTGTGTATGTACTCAATGACGCCAGATGAAGACTTTGTGATTGATTTTTTGGGAGGAGAATTTGGGAAGGATGTGGTAATCGGCGGTGGGTTTTCGGGGCACGGGTTCAAAATGTCGCCAGCGATCGGGAGGATACTGGCAGATCTTGCATTGAAGGGGGTGGCGGAGGGGTTGGAGCTGAAGTATTTCAGGATAGCAAGGTTTGAGGAGAATCCAAAAGGTAATGTCAAGGGCTTTGCAGATCAAGTAAGGCTTCACTAG

Coding sequence (CDS)

ATGGGTATTTTCGTCATTTCATTAAAACATGAGCTTCCTACACGAAGGTGCAGTAGCACACTTCAATTCAACTTCAATAACTCTCTTCCATTACTCTACACGCAAATTCCCCTCATCGGAGACTCGGAGGAGAGGAAAAAAAGTTCCAAACAAATTGGAGGAGAAGAAATGGCCGATTCCGGCACTCAATATGATGTAATCGTCGTCGGCGCCGGCGTAATGGGCAGTTCGACGGCATACCATCTCGCTAAAACAGGGAACAAAGTTCTGATTCTGGAGCAGTTCGATTTTCTTCACCATAGAGGCTCTTCTCATGGAGAATCTCGCACCATACGCGCCACCTATCCGGAGGATTATTATCACAGTCTCGTTATGGAATCTTACGAGCTCTGGCGGACGGCGGAGGCAGAAATTGGTTACAGGGTATATTTTCCGGCGGAGCAGCTCGATATCGGCCATTCCGACGATAAAAGTCTCGCCGCCGCCGTCGATACCTGCAAGAAGCATTCGATCCCGCATCTGGTTCTGGATCGGGAGCAACTGGCGGAGAAGTACTCTGGCAGGGTGGAGATCCCGGCGGACTGGGTGGCGGTGTGGAGCAAGTACGGTGGCGTAATTAAGCCGACGAAGGCGGTTTCGATGTTCCAAACTCTGGCGTTCAAAAACGGCGCTGATTTGAAGGACAACGCGGAAGTGGTGGAGATAAAGAGAGATGGGAGTAGTGGAGGAGTAGTTGTTTCAGTAGCCAATGGGGAGAGATTTAGGGGAAAGAAATGTGTGGTGACAGTTGGAGCTTGGGCTAGAAAGTTAGTTAAATCAGTTGGTGGGATTGAATTGCCAATTCAACCATTGGAGGCTACTGTGTCCTACTGGAGGATCAAGGAGGGAGCTGAGGGGGAGTATGCAATCGGCGGGAACTTTCCGACGTTTGCTAGCTACGGTGACACATATATCTACGGAACGCCATCGTTGGAGTTTCCGGGATTGATTAAGGTAGCCGTGCATGGCGGGCATCGGTGCGACCCGGACAAACGGTCATGGGGGAGTGGGGGGCAGCTGCCGATAACTGCATTAAAGAAGTGGATAGAGGGGAGGTTTGGGGGGAGGGTGGATTCAAGTGAGCCAGTGTCGACACAACTGTGTATGTACTCAATGACGCCAGATGAAGACTTTGTGATTGATTTTTTGGGAGGAGAATTTGGGAAGGATGTGGTAATCGGCGGTGGGTTTTCGGGGCACGGGTTCAAAATGTCGCCAGCGATCGGGAGGATACTGGCAGATCTTGCATTGAAGGGGGTGGCGGAGGGGTTGGAGCTGAAGTATTTCAGGATAGCAAGGTTTGAGGAGAATCCAAAAGGTAATGTCAAGGGCTTTGCAGATCAAGTAAGGCTTCACTAG

Protein sequence

MGIFVISLKHELPTRRCSSTLQFNFNNSLPLLYTQIPLIGDSEERKKSSKQIGGEEMADSGTQYDVIVVGAGVMGSSTAYHLAKTGNKVLILEQFDFLHHRGSSHGESRTIRATYPEDYYHSLVMESYELWRTAEAEIGYRVYFPAEQLDIGHSDDKSLAAAVDTCKKHSIPHLVLDREQLAEKYSGRVEIPADWVAVWSKYGGVIKPTKAVSMFQTLAFKNGADLKDNAEVVEIKRDGSSGGVVVSVANGERFRGKKCVVTVGAWARKLVKSVGGIELPIQPLEATVSYWRIKEGAEGEYAIGGNFPTFASYGDTYIYGTPSLEFPGLIKVAVHGGHRCDPDKRSWGSGGQLPITALKKWIEGRFGGRVDSSEPVSTQLCMYSMTPDEDFVIDFLGGEFGKDVVIGGGFSGHGFKMSPAIGRILADLALKGVAEGLELKYFRIARFEENPKGNVKGFADQVRLH

Homology

[Close]

BLAST of CmaCh09G005320 vs. ExPASy Swiss-Prot
Match: Q9SJA7 (Probable sarcosine oxidase OS=Arabidopsis thaliana OX=3702 GN=At2g24580 PE=2 SV=1)

HSP 1 Score: 568.9 bits (1465), Expect = 5.2e-161
Identity = 269/412 (65.29%), Postives = 329/412 (79.85%), Query Frame = 0


Query: 56  EMADSGTQYDVIVVGAGVMGSSTAYHLAKTGNKVLILEQFDFLHHRGSSHGESRTIRATY 115
           E +D G ++DVIVVGAGVMGSS AY LAK G K L+LEQFDFLHHRGSSHGESRTIRATY
Sbjct: 2   EYSDDG-RFDVIVVGAGVMGSSAAYQLAKRGQKTLLLEQFDFLHHRGSSHGESRTIRATY 61

Query: 116 PEDYYHSLVMESYELWRTAEAEIGYRVYFPAEQLDIGHSDDKSLAAAVDTCKKHSIPHLV 175
           PEDYY+S+V ES  LW  A++EIGY+V+FP +Q D+G +D +SL + V TC+KH + H V
Sbjct: 62  PEDYYYSMVSESTRLWAAAQSEIGYKVHFPTQQFDMGPADQQSLLSVVATCQKHGLAHRV 121

Query: 176 LDREQLAEKYSGRVEIPADWVAVWSKYGGVIKPTKAVSMFQTLAFKNGADLKDNAEVVEI 235
           +D   ++E +SGR+ IP +W+ V ++ GG+IKPTKAVSMFQTLA  +GA L+DN +V  I
Sbjct: 122 MDSHAVSEHFSGRISIPENWIGVSTELGGIIKPTKAVSMFQTLAIGHGAILRDNTKVANI 181

Query: 236 KRDGSSG-GVVVSVANGERFRGKKCVVTVGAWARKLVKSVGGIELPIQPLEATVSYWRIK 295
           KRDG SG GV+V    G++F GKKC+VT GAW  KLVK+V GI+ P++PLE TV YWRIK
Sbjct: 182 KRDGESGEGVIVCTVKGDKFYGKKCIVTAGAWISKLVKTVAGIDFPVEPLETTVCYWRIK 241

Query: 296 EGAEGEYAIGGNFPTFASYGDTYIYGTPSLEFPGLIKVAVHGGHRCDPDKRSWGSGGQLP 355
           EG E ++ I G FPTFASYG  Y+YGTPSLE+PGLIKVAVHGG+ CDPDKR WG G  + 
Sbjct: 242 EGHEEKFTIDGEFPTFASYGAPYVYGTPSLEYPGLIKVAVHGGYWCDPDKRPWGPG--VK 301

Query: 356 ITALKKWIEGRFGGRVDSSEPVSTQLCMYSMTPDEDFVIDFLGGEFGKDVVIGGGFSGHG 415
           +  LK+WI+ RFGG VDS  PV+TQLCMYSMTPDEDFVIDFLGGEFG+DVV+GGGFSGHG
Sbjct: 302 LEELKEWIKERFGGMVDSEGPVATQLCMYSMTPDEDFVIDFLGGEFGRDVVVGGGFSGHG 361

Query: 416 FKMSPAIGRILADLALKGVA--EGLELKYFRIARFEENPKGNVKGFADQVRL 465
           FKM+PA+GRILAD+A++  A   G+E+K F + RFE+NPKGN K + DQV L
Sbjct: 362 FKMAPAVGRILADMAMEVEAGGGGVEMKQFSLRRFEDNPKGNAKEYPDQVIL 410

[Close]

BLAST of CmaCh09G005320 vs. ExPASy Swiss-Prot
Match: Q29RU9 (Peroxisomal sarcosine oxidase OS=Bos taurus OX=9913 GN=PIPOX PE=2 SV=2)

HSP 1 Score: 246.9 bits (629), Expect = 4.5e-64
Identity = 147/405 (36.30%), Postives = 212/405 (52.35%), Query Frame = 0


Query: 57  MADSGTQYDVIVVGAGVMGSSTAYHLAKTGNKVLILEQFDFLHHRGSSHGESRTIRATYP 116
           MA     YD IV+GAG+ G  TAYHLAK   KVL+LEQF   H RGSSHG+SR IR  YP
Sbjct: 1   MAAQRELYDAIVIGAGIQGCFTAYHLAKHSKKVLLLEQFFLPHSRGSSHGQSRIIRRAYP 60

Query: 117 EDYYHSLVMESYELWRTAEAEIGYRVYFPAEQLDIGHSDDKSLAAAVDTCKKHSIPHLVL 176
           ED+Y  ++ E Y LW   E E G ++Y     L +G  ++  L     T  +  + H  L
Sbjct: 61  EDFYTQMMAECYSLWAQLEHEAGTQLYRQTGLLLLGMKENPELKIIQATLSRQGVEHQCL 120

Query: 177 DREQLAEKYSGRVEIPADWVAVWSKYGGVIKPTKAVSMFQTLAFKNGADLKDNAEVVEIK 236
             E+L +++   + +    V +    GGV+   KA+   Q    + G  + D  +VVEIK
Sbjct: 121 SSEELKQRFP-NIRLARGEVGLLEVSGGVLYADKALRALQDAIRQLGGIVHDGEKVVEIK 180

Query: 237 RDGSSGGVVVSVANGERFRGKKCVVTVGAWARKLVKSVGGIELPIQPLEATVSYWRIKEG 296
               SG  V+       ++ K  ++T G W  +L++ +G  ELP+Q L   V YW  +E 
Sbjct: 181 ----SGLPVMVKTTSRSYQAKSLIITAGPWTNRLLRPLGA-ELPLQTLRINVCYW--QEK 240

Query: 297 AEGEYAIGGNFPTFASYG----DTYIYGTPSLEFPGLIKVAVHGGHRCDPDKRSWGSGGQ 356
             G Y++   FP F   G      +IYG PS E+PGL+KV  H G+  DP++R   +   
Sbjct: 241 VPGSYSVSQAFPCFMGLGLSLAPHHIYGLPSREYPGLMKVCYHHGNNADPEERDCPAAFS 300

Query: 357 --LPITALKKWIEGRFGGRVDSSEPVSTQLCMYSMTPDEDFVIDFLGGEFGKDVVIGGGF 416
               +  L  ++           EP   + CMY+ TPD  FV+D        ++VIG GF
Sbjct: 301 DIQDVHILSGFVRDHLPDL--QPEPAVMEHCMYTNTPDGHFVLD--RHPKYDNIVIGAGF 360

Query: 417 SGHGFKMSPAIGRILADLALKGVAEGLELKYFRIARFEENPKGNV 456
           SGHGFK+SP +G+IL +L++K +    +L  FRI+RF    K ++
Sbjct: 361 SGHGFKLSPVVGKILYELSMK-LTPSYDLTPFRISRFPSLGKAHL 392

[Close]

BLAST of CmaCh09G005320 vs. ExPASy Swiss-Prot
Match: Q9P0Z9 (Peroxisomal sarcosine oxidase OS=Homo sapiens OX=9606 GN=PIPOX PE=1 SV=2)

HSP 1 Score: 240.7 bits (613), Expect = 3.2e-62
Identity = 144/406 (35.47%), Postives = 221/406 (54.43%), Query Frame = 0


Query: 57  MADSGTQYDVIVVGAGVMGSSTAYHLAKTGNKVLILEQFDFLHHRGSSHGESRTIRATYP 116
           MA     +D IV+GAG+ G  TAYHLAK   ++L+LEQF   H RGSSHG+SR IR  Y 
Sbjct: 1   MAAQKDLWDAIVIGAGIQGCFTAYHLAKHRKRILLLEQFFLPHSRGSSHGQSRIIRKAYL 60

Query: 117 EDYYHSLVMESYELWRTAEAEIGYRVYFPAEQLDIGHSDDKSLAAAVDTCKKHSIPHLVL 176
           ED+Y  ++ E Y++W   E E G +++     L +G  +++ L        +  + H  L
Sbjct: 61  EDFYTRMMHECYQIWAQLEHEAGTQLHRQTGLLLLGMKENQELKTIQANLSRQRVEHQCL 120

Query: 177 DREQLAEKYSGRVEIPADWVAVWSKYGGVIKPTKAVSMFQTLAFKNGADLKDNAEVVEIK 236
             E+L +++   + +P   V +    GGVI   KA+   Q    + G  ++D  +VVEI 
Sbjct: 121 SSEELKQRFP-NIRLPRGEVGLLDNSGGVIYAYKALRALQDAIRQLGGIVRDGEKVVEI- 180

Query: 237 RDGSSGGVVVSVANGER-FRGKKCVVTVGAWARKLVKSVGGIELPIQPLEATVSYWRIKE 296
               + G++V+V    R ++ K  V+T G W  +L++ + GIE+P+Q L   V YWR  E
Sbjct: 181 ----NPGLLVTVKTTSRSYQAKSLVITAGPWTNQLLRPL-GIEMPLQTLRINVCYWR--E 240

Query: 297 GAEGEYAIGGNFPTFASYG--DTYIYGTPSLEFPGLIKVAVHGGHRCDPDKRSWGSG--- 356
              G Y +   FP F   G    +IYG P+ E+PGL+KV+ H G+  DP++R   +    
Sbjct: 241 MVPGSYGVSQAFPCFLWLGLCPHHIYGLPTGEYPGLMKVSYHHGNHADPEERDCPTARTD 300

Query: 357 -GQLPITALKKWIEGRFGGRVDSSEPVSTQLCMYSMTPDEDFVIDFLGGEFGKDVVIGGG 416
            G + I  L  ++           EP   + CMY+ TPDE F++D        ++VIG G
Sbjct: 301 IGDVQI--LSSFVRDHLPDL--KPEPAVIESCMYTNTPDEQFILD--RHPKYDNIVIGAG 360

Query: 417 FSGHGFKMSPAIGRILADLALKGVAEGLELKYFRIARFEENPKGNV 456
           FSGHGFK++P +G+IL +L++K +    +L  FRI+RF    K ++
Sbjct: 361 FSGHGFKLAPVVGKILYELSMK-LTPSYDLAPFRISRFPSLGKAHL 390

[Close]

BLAST of CmaCh09G005320 vs. ExPASy Swiss-Prot
Match: P79371 (Peroxisomal sarcosine oxidase OS=Oryctolagus cuniculus OX=9986 GN=PIPOX PE=1 SV=1)

HSP 1 Score: 237.7 bits (605), Expect = 2.7e-61
Identity = 142/406 (34.98%), Postives = 219/406 (53.94%), Query Frame = 0


Query: 57  MADSGTQYDVIVVGAGVMGSSTAYHLAKTGNKVLILEQFDFLHHRGSSHGESRTIRATYP 116
           MA     +D IV+GAG+ G  T YHL K   ++L+LEQF   H RGSSHG+SR IR  Y 
Sbjct: 1   MAAQKDLWDAIVIGAGIQGCFTVYHLVKHRKRILLLEQFFLPHSRGSSHGQSRIIRKAYL 60

Query: 117 EDYYHSLVMESYELWRTAEAEIGYRVYFPAEQLDIGHSDDKSLAAAVDTCKKHSIPHLVL 176
           ED+Y  ++ E Y++W   E E G +++     L +G  +++ L        +  + H  L
Sbjct: 61  EDFYTRMMHECYQIWAQLEHEAGTQLHRQTGLLLLGMKENQELKTIQANLSRQRVEHQCL 120

Query: 177 DREQLAEKYSGRVEIPADWVAVWSKYGGVIKPTKAVSMFQTLAFKNGADLKDNAEVVEIK 236
             E+L +++   + +P   V +    GGVI   KA+   Q    + G  ++D  +VVEI 
Sbjct: 121 SSEELKQRFP-NIRLPRGEVGLLDNSGGVIYAYKALRALQDAIRQLGGIVRDGEKVVEI- 180

Query: 237 RDGSSGGVVVSVANGER-FRGKKCVVTVGAWARKLVKSVGGIELPIQPLEATVSYWRIKE 296
               + G++V+V    R ++ K  V+T G W  +L++ + GIE+P+Q L   V YWR  E
Sbjct: 181 ----NPGLLVTVKTTSRSYQAKSLVITAGPWTNQLLRPL-GIEMPLQTLRINVCYWR--E 240

Query: 297 GAEGEYAIGGNFPTFASYG--DTYIYGTPSLEFPGLIKVAVHGGHRCDPDKRSWGSG--- 356
              G Y +   FP F   G    +IYG P+ E+PGL+KV+ H G+  DP++R   +    
Sbjct: 241 MVPGSYGVSQAFPCFLWLGLCPHHIYGLPTGEYPGLMKVSYHHGNHADPEERDCPTARTD 300

Query: 357 -GQLPITALKKWIEGRFGGRVDSSEPVSTQLCMYSMTPDEDFVIDFLGGEFGKDVVIGGG 416
            G + I  L  ++           EP   + CMY+ TPDE F++D        ++VIG G
Sbjct: 301 IGDVQI--LSSFVRDHLPDL--KPEPAVIESCMYTNTPDEQFILD--RHPKYDNIVIGAG 360

Query: 417 FSGHGFKMSPAIGRILADLALKGVAEGLELKYFRIARFEENPKGNV 456
           FSGHGFK++P +G+IL +L++K +    +L  FRI+RF    K ++
Sbjct: 361 FSGHGFKLAPVVGKILYELSMK-LTPSYDLAPFRISRFPSLGKAHL 390

[Close]

BLAST of CmaCh09G005320 vs. ExPASy Swiss-Prot
Match: Q9D826 (Peroxisomal sarcosine oxidase OS=Mus musculus OX=10090 GN=Pipox PE=1 SV=1)

HSP 1 Score: 232.3 bits (591), Expect = 1.1e-59
Identity = 141/404 (34.90%), Postives = 210/404 (51.98%), Query Frame = 0


Query: 57  MADSGTQYDVIVVGAGVMGSSTAYHLAKTGNKVLILEQFDFLHHRGSSHGESRTIRATYP 116
           MA     +D IV+GAG+ G  TAYHLAK    VL+LEQF   H RGSSHG+SR IR  YP
Sbjct: 1   MAAQTDFWDAIVIGAGIQGCFTAYHLAKHSKSVLLLEQFFLPHSRGSSHGQSRIIRKAYP 60

Query: 117 EDYYHSLVMESYELWRTAEAEIGYRVYFPAEQLDIGHSDDKSLAAAVDTCKKHSIPHLVL 176
           ED+Y  ++ E Y+ W   E E G +++   E L +G  ++  L     T  +  I H  L
Sbjct: 61  EDFYTMMMKECYQTWAQLEREAGTQLHRQTELLLLGTKENPGLKTIQATLSRQGIDHEYL 120

Query: 177 DREQLAEKYSGRVEIPADWVAVWSKYGGVIKPTKAVSMFQTLAFKNGADLKDNAEVVEIK 236
               L +++   +      V +  K GGV+   KA+   Q +  + G  + D  +VVEI+
Sbjct: 121 SSVDLKQRFP-NIRFTRGEVGLLDKTGGVLYADKALRALQHIICQLGGTVCDGEKVVEIR 180

Query: 237 RDGSSGGVVVSVANG-ERFRGKKCVVTVGAWARKLVKSVGGIELPIQPLEATVSYWRIKE 296
                 G+ V+V    + ++    V+T G W  +L+  + GIELP+Q L   V YWR  E
Sbjct: 181 -----PGLPVTVKTTLKSYQANSLVITAGPWTNRLLHPL-GIELPLQTLRINVCYWR--E 240

Query: 297 GAEGEYAIGGNFPTF--ASYGDTYIYGTPSLEFPGLIKVAVHGGHRCDPDKRSWGS--GG 356
              G Y +   FP          +IYG P+ E+PGL+K+  H G   DP++R        
Sbjct: 241 KVPGSYGVSQAFPCILGLDLAPHHIYGLPASEYPGLMKICYHHGDNVDPEERDCPKTFSD 300

Query: 357 QLPITALKKWIEGRFGGRVDSSEPVSTQLCMYSMTPDEDFVIDFLGGEFGKDVVIGGGFS 416
              +  L  ++     G    +EP   + CMY+ TPDE F++D        ++VIG GFS
Sbjct: 301 IQDVQILCHFVRDHLPGL--RAEPDIMERCMYTNTPDEHFILDC--HPKYDNIVIGAGFS 360

Query: 417 GHGFKMSPAIGRILADLALKGVAEGLELKYFRIARFEENPKGNV 456
           GHGFK++P +G+IL +L++K +    +L  FR++RF    K ++
Sbjct: 361 GHGFKLAPVVGKILYELSMK-LPPSYDLAPFRMSRFSTLSKAHL 390

[Close]

BLAST of CmaCh09G005320 vs. ExPASy TrEMBL
Match: A0A6J1IIQ6 (Sarcosine oxidase OS=Cucurbita maxima OX=3661 GN=LOC111474391 PE=3 SV=1)

HSP 1 Score: 840.9 bits (2171), Expect = 2.6e-240
Identity = 409/409 (100.00%), Postives = 409/409 (100.00%), Query Frame = 0


Query: 57  MADSGTQYDVIVVGAGVMGSSTAYHLAKTGNKVLILEQFDFLHHRGSSHGESRTIRATYP 116
           MADSGTQYDVIVVGAGVMGSSTAYHLAKTGNKVLILEQFDFLHHRGSSHGESRTIRATYP
Sbjct: 1   MADSGTQYDVIVVGAGVMGSSTAYHLAKTGNKVLILEQFDFLHHRGSSHGESRTIRATYP 60

Query: 117 EDYYHSLVMESYELWRTAEAEIGYRVYFPAEQLDIGHSDDKSLAAAVDTCKKHSIPHLVL 176
           EDYYHSLVMESYELWRTAEAEIGYRVYFPAEQLDIGHSDDKSLAAAVDTCKKHSIPHLVL
Sbjct: 61  EDYYHSLVMESYELWRTAEAEIGYRVYFPAEQLDIGHSDDKSLAAAVDTCKKHSIPHLVL 120

Query: 177 DREQLAEKYSGRVEIPADWVAVWSKYGGVIKPTKAVSMFQTLAFKNGADLKDNAEVVEIK 236
           DREQLAEKYSGRVEIPADWVAVWSKYGGVIKPTKAVSMFQTLAFKNGADLKDNAEVVEIK
Sbjct: 121 DREQLAEKYSGRVEIPADWVAVWSKYGGVIKPTKAVSMFQTLAFKNGADLKDNAEVVEIK 180

Query: 237 RDGSSGGVVVSVANGERFRGKKCVVTVGAWARKLVKSVGGIELPIQPLEATVSYWRIKEG 296
           RDGSSGGVVVSVANGERFRGKKCVVTVGAWARKLVKSVGGIELPIQPLEATVSYWRIKEG
Sbjct: 181 RDGSSGGVVVSVANGERFRGKKCVVTVGAWARKLVKSVGGIELPIQPLEATVSYWRIKEG 240

Query: 297 AEGEYAIGGNFPTFASYGDTYIYGTPSLEFPGLIKVAVHGGHRCDPDKRSWGSGGQLPIT 356
           AEGEYAIGGNFPTFASYGDTYIYGTPSLEFPGLIKVAVHGGHRCDPDKRSWGSGGQLPIT
Sbjct: 241 AEGEYAIGGNFPTFASYGDTYIYGTPSLEFPGLIKVAVHGGHRCDPDKRSWGSGGQLPIT 300

Query: 357 ALKKWIEGRFGGRVDSSEPVSTQLCMYSMTPDEDFVIDFLGGEFGKDVVIGGGFSGHGFK 416
           ALKKWIEGRFGGRVDSSEPVSTQLCMYSMTPDEDFVIDFLGGEFGKDVVIGGGFSGHGFK
Sbjct: 301 ALKKWIEGRFGGRVDSSEPVSTQLCMYSMTPDEDFVIDFLGGEFGKDVVIGGGFSGHGFK 360

Query: 417 MSPAIGRILADLALKGVAEGLELKYFRIARFEENPKGNVKGFADQVRLH 466
           MSPAIGRILADLALKGVAEGLELKYFRIARFEENPKGNVKGFADQVRLH
Sbjct: 361 MSPAIGRILADLALKGVAEGLELKYFRIARFEENPKGNVKGFADQVRLH 409

[Close]

BLAST of CmaCh09G005320 vs. ExPASy TrEMBL
Match: A0A6J1FC48 (Sarcosine oxidase OS=Cucurbita moschata OX=3662 GN=LOC111442750 PE=3 SV=1)

HSP 1 Score: 828.9 bits (2140), Expect = 1.0e-236
Identity = 402/409 (98.29%), Postives = 406/409 (99.27%), Query Frame = 0


Query: 57  MADSGTQYDVIVVGAGVMGSSTAYHLAKTGNKVLILEQFDFLHHRGSSHGESRTIRATYP 116
           MADSGTQYDVIVVGAGVMGSSTAYHLAKTGNKVL LEQFDFLHHRGSSHGESRTIRATYP
Sbjct: 1   MADSGTQYDVIVVGAGVMGSSTAYHLAKTGNKVLTLEQFDFLHHRGSSHGESRTIRATYP 60

Query: 117 EDYYHSLVMESYELWRTAEAEIGYRVYFPAEQLDIGHSDDKSLAAAVDTCKKHSIPHLVL 176
           EDYYHSLVMESYELWRTAEAEIGYRVYFPAEQLDIGHSDDKSLAAAVDTCKKHSIPHLVL
Sbjct: 61  EDYYHSLVMESYELWRTAEAEIGYRVYFPAEQLDIGHSDDKSLAAAVDTCKKHSIPHLVL 120

Query: 177 DREQLAEKYSGRVEIPADWVAVWSKYGGVIKPTKAVSMFQTLAFKNGADLKDNAEVVEIK 236
           DREQLAEKYSGRVEIPADWVAVWSKYGGVIKPTKAVSMFQTLAFKNGADLKDNAEVVEIK
Sbjct: 121 DREQLAEKYSGRVEIPADWVAVWSKYGGVIKPTKAVSMFQTLAFKNGADLKDNAEVVEIK 180

Query: 237 RDGSSGGVVVSVANGERFRGKKCVVTVGAWARKLVKSVGGIELPIQPLEATVSYWRIKEG 296
           R+GSSGGVVVS ANGERFRGKKCVVTVGAWARKLVKSVGGIELPIQPLEATVSYWRIKEG
Sbjct: 181 REGSSGGVVVSTANGERFRGKKCVVTVGAWARKLVKSVGGIELPIQPLEATVSYWRIKEG 240

Query: 297 AEGEYAIGGNFPTFASYGDTYIYGTPSLEFPGLIKVAVHGGHRCDPDKRSWGSGGQLPIT 356
           AEGEYAIGG+FPTFASYGDTYIYGTPSLEFPGLIKVAVHGGHRCDPDKRSWGSGG+LPIT
Sbjct: 241 AEGEYAIGGDFPTFASYGDTYIYGTPSLEFPGLIKVAVHGGHRCDPDKRSWGSGGELPIT 300

Query: 357 ALKKWIEGRFGGRVDSSEPVSTQLCMYSMTPDEDFVIDFLGGEFGKDVVIGGGFSGHGFK 416
           ALK+WIEGRFGGRVDSSEPVSTQLCMYSMTPDEDFVIDFLGGEFGKDVVIGGGFSGHGFK
Sbjct: 301 ALKEWIEGRFGGRVDSSEPVSTQLCMYSMTPDEDFVIDFLGGEFGKDVVIGGGFSGHGFK 360

Query: 417 MSPAIGRILADLALKGVAEGLELKYFRIARFEENPKGNVKGFADQVRLH 466
           MSPAIGRILADLALKGVAEGLELKYFRIARFEENPKGNVK FADQVRLH
Sbjct: 361 MSPAIGRILADLALKGVAEGLELKYFRIARFEENPKGNVKSFADQVRLH 409

[Close]

BLAST of CmaCh09G005320 vs. ExPASy TrEMBL
Match: A0A0A0LJG6 (Sarcosine oxidase OS=Cucumis sativus OX=3659 GN=Csa_3G881890 PE=3 SV=1)

HSP 1 Score: 721.8 bits (1862), Expect = 1.8e-204
Identity = 343/409 (83.86%), Postives = 372/409 (90.95%), Query Frame = 0


Query: 57  MADSGTQYDVIVVGAGVMGSSTAYHLAKTGNKVLILEQFDFLHHRGSSHGESRTIRATYP 116
           MA S T +DVIVVGAGVMGSSTAYHLAKTGN+VLILEQFDFLHHRGSSHGESRTIRATYP
Sbjct: 1   MAASDTLFDVIVVGAGVMGSSTAYHLAKTGNRVLILEQFDFLHHRGSSHGESRTIRATYP 60

Query: 117 EDYYHSLVMESYELWRTAEAEIGYRVYFPAEQLDIGHSDDKSLAAAVDTCKKHSIPHLVL 176
           EDYY+ LVMESYELWR AE EIGY+VYFP EQLDIG  DDKSL A VDTC+KHSIPHLVL
Sbjct: 61  EDYYYGLVMESYELWRMAEEEIGYKVYFPTEQLDIGSPDDKSLTAVVDTCRKHSIPHLVL 120

Query: 177 DREQLAEKYSGRVEIPADWVAVWSKYGGVIKPTKAVSMFQTLAFKNGADLKDNAEVVEIK 236
           D  +L EKYSGRVEIPADWV VWSKYGGVIKPTKAVSM+QTLA+KNGA +KDNAEVVEIK
Sbjct: 121 DSGELREKYSGRVEIPADWVGVWSKYGGVIKPTKAVSMYQTLAYKNGAVMKDNAEVVEIK 180

Query: 237 RDGSSGGVVVSVANGERFRGKKCVVTVGAWARKLVKSVGGIELPIQPLEATVSYWRIKEG 296
           RD S+G +VVS+ANGE FRGKKCVVTVGAW++KLVKSVGGIELPI+PLE +VSYWRIKEG
Sbjct: 181 RDESNGRIVVSIANGESFRGKKCVVTVGAWSKKLVKSVGGIELPIRPLEVSVSYWRIKEG 240

Query: 297 AEGEYAIGGNFPTFASYGDTYIYGTPSLEFPGLIKVAVHGGHRCDPDKRSWGSGGQLPIT 356
            E EYAIGG FPT ASYG+ Y+YGTPSLEFPGLIKVA+HGGH C+PDKRSWG GG+LPI 
Sbjct: 241 FEAEYAIGGGFPTIASYGEPYVYGTPSLEFPGLIKVAIHGGHECNPDKRSWGKGGRLPIA 300

Query: 357 ALKKWIEGRFGGRVDSSEPVSTQLCMYSMTPDEDFVIDFLGGEFGKDVVIGGGFSGHGFK 416
           ALK+WI+ +FGGRVDSS PVSTQ CMYSMTPD DFVIDFLGGEF KDVVIGGGFSGHGFK
Sbjct: 301 ALKEWIDEKFGGRVDSSGPVSTQSCMYSMTPDGDFVIDFLGGEFEKDVVIGGGFSGHGFK 360

Query: 417 MSPAIGRILADLALKGVAEGLELKYFRIARFEENPKGNVKGFADQVRLH 466
           MSP IGRILA+LAL G AEG+ELKYF++ARFEENPKGNVK FADQV+LH
Sbjct: 361 MSPTIGRILAELALDGAAEGVELKYFKLARFEENPKGNVKSFADQVKLH 409

[Close]

BLAST of CmaCh09G005320 vs. ExPASy TrEMBL
Match: A0A5D3E5F8 (Sarcosine oxidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold455G006680 PE=3 SV=1)

HSP 1 Score: 711.1 bits (1834), Expect = 3.1e-201
Identity = 341/409 (83.37%), Postives = 372/409 (90.95%), Query Frame = 0


Query: 57  MADSGTQYDVIVVGAGVMGSSTAYHLAKTGNKVLILEQFDFLHHRGSSHGESRTIRATYP 116
           MA S TQ+DVIVVGAGVMGSSTAYHLAKTGN+VL+LEQFDFLHHRGSSHGESRTIRATYP
Sbjct: 1   MAASDTQFDVIVVGAGVMGSSTAYHLAKTGNRVLLLEQFDFLHHRGSSHGESRTIRATYP 60

Query: 117 EDYYHSLVMESYELWRTAEAEIGYRVYFPAEQLDIGHSDDKSLAAAVDTCKKHSIPHLVL 176
           EDYYH LVMESYELWR AEAEIG++VY+PAEQLDIG S+ +SL A VDTC KHSIPHLVL
Sbjct: 61  EDYYHDLVMESYELWRMAEAEIGFKVYYPAEQLDIGPSNSESLTAVVDTCLKHSIPHLVL 120

Query: 177 DREQLAEKYSGRVEIPADWVAVWSKYGGVIKPTKAVSMFQTLAFKNGADLKDNAEVVEIK 236
           DR +L EKYSGRVEIPA+WVAV SKYGGVIKPTKAVSMFQTLA+KNG  LKDNAEVVEIK
Sbjct: 121 DRGELMEKYSGRVEIPANWVAVCSKYGGVIKPTKAVSMFQTLAYKNGTVLKDNAEVVEIK 180

Query: 237 RDGSSGGVVVSVANGERFRGKKCVVTVGAWARKLVKSVGGIELPIQPLEATVSYWRIKEG 296
           RD S+G +VVS ANGE F GKKCVVTVGAW++KLVKSVGGIELPI PLE +VSYWRIKEG
Sbjct: 181 RDESNGRIVVSTANGESFHGKKCVVTVGAWSKKLVKSVGGIELPILPLEVSVSYWRIKEG 240

Query: 297 AEGEYAIGGNFPTFASYGDTYIYGTPSLEFPGLIKVAVHGGHRCDPDKRSWGSGGQLPIT 356
            E EYAI G FPT ASYG+ Y+YGTPSLEFPGLIKVA+H G+ C+PDKRSWG  G+LPI 
Sbjct: 241 FEAEYAIEGGFPTMASYGEPYVYGTPSLEFPGLIKVAIHAGYSCNPDKRSWGRDGRLPIA 300

Query: 357 ALKKWIEGRFGGRVDSSEPVSTQLCMYSMTPDEDFVIDFLGGEFGKDVVIGGGFSGHGFK 416
           ALK+WI+ +FGGRVDSS PVS+QLCMYSMTPDEDFVIDFLGGEF KDVVIGGGFSGHGFK
Sbjct: 301 ALKEWIDEKFGGRVDSSRPVSSQLCMYSMTPDEDFVIDFLGGEFEKDVVIGGGFSGHGFK 360

Query: 417 MSPAIGRILADLALKGVAEGLELKYFRIARFEENPKGNVKGFADQVRLH 466
           MSPAIGRILA+LALKG AEG+ELKYF++ARFEENPKGNVK FADQV+LH
Sbjct: 361 MSPAIGRILAELALKGAAEGVELKYFKLARFEENPKGNVKSFADQVKLH 409

[Close]

BLAST of CmaCh09G005320 vs. ExPASy TrEMBL
Match: A0A1S3CR44 (Sarcosine oxidase OS=Cucumis melo OX=3656 GN=LOC103503750 PE=3 SV=1)

HSP 1 Score: 710.7 bits (1833), Expect = 4.1e-201
Identity = 341/409 (83.37%), Postives = 372/409 (90.95%), Query Frame = 0


Query: 57  MADSGTQYDVIVVGAGVMGSSTAYHLAKTGNKVLILEQFDFLHHRGSSHGESRTIRATYP 116
           MA S TQ+DVIVVGAGVMGSSTAYHLAKTGN+VL+LEQFDFLHHRGSSHGESRTIRATYP
Sbjct: 1   MAASDTQFDVIVVGAGVMGSSTAYHLAKTGNRVLLLEQFDFLHHRGSSHGESRTIRATYP 60

Query: 117 EDYYHSLVMESYELWRTAEAEIGYRVYFPAEQLDIGHSDDKSLAAAVDTCKKHSIPHLVL 176
           EDYYH LVMESYELWR AEAEIG++VY+PAEQLDIG S+ +SL A VDTC KHSIPHLVL
Sbjct: 61  EDYYHDLVMESYELWRMAEAEIGFKVYYPAEQLDIGPSNSESLTAVVDTCLKHSIPHLVL 120

Query: 177 DREQLAEKYSGRVEIPADWVAVWSKYGGVIKPTKAVSMFQTLAFKNGADLKDNAEVVEIK 236
           DR +L EKYSGRVEIPA+WVAV SKYGGVIKPTKAVSMFQTLA+KNG  LKDNAEVVEIK
Sbjct: 121 DRGELMEKYSGRVEIPANWVAVCSKYGGVIKPTKAVSMFQTLAYKNGTVLKDNAEVVEIK 180

Query: 237 RDGSSGGVVVSVANGERFRGKKCVVTVGAWARKLVKSVGGIELPIQPLEATVSYWRIKEG 296
           RD S+G +VVS ANGE F GKKCVVTVGAW++KLVKSVGGIELPI PLE +VSYWRIKEG
Sbjct: 181 RDESNGRIVVSTANGESFHGKKCVVTVGAWSKKLVKSVGGIELPILPLEVSVSYWRIKEG 240

Query: 297 AEGEYAIGGNFPTFASYGDTYIYGTPSLEFPGLIKVAVHGGHRCDPDKRSWGSGGQLPIT 356
            E EYAI G FPT ASYG+ Y+YGTPSLEFPGLIKVA+H G+ C+PDKRSWG  G+LPI 
Sbjct: 241 FEAEYAIEGGFPTMASYGEPYVYGTPSLEFPGLIKVAIHAGYSCNPDKRSWGREGRLPIA 300

Query: 357 ALKKWIEGRFGGRVDSSEPVSTQLCMYSMTPDEDFVIDFLGGEFGKDVVIGGGFSGHGFK 416
           ALK+WI+ +FGGRVDSS PVS+QLCMYSMTPDEDFVIDFLGGEF KDVVIGGGFSGHGFK
Sbjct: 301 ALKEWIDEKFGGRVDSSRPVSSQLCMYSMTPDEDFVIDFLGGEFEKDVVIGGGFSGHGFK 360

Query: 417 MSPAIGRILADLALKGVAEGLELKYFRIARFEENPKGNVKGFADQVRLH 466
           MSPAIGRILA+LALKG AEG+ELKYF++ARFEENPKGNVK FADQV+LH
Sbjct: 361 MSPAIGRILAELALKGAAEGVELKYFKLARFEENPKGNVKSFADQVKLH 409

[Close]

BLAST of CmaCh09G005320 vs. NCBI nr
Match: XP_022975268.1 (probable sarcosine oxidase [Cucurbita maxima])

HSP 1 Score: 840.9 bits (2171), Expect = 5.4e-240
Identity = 409/409 (100.00%), Postives = 409/409 (100.00%), Query Frame = 0


Query: 57  MADSGTQYDVIVVGAGVMGSSTAYHLAKTGNKVLILEQFDFLHHRGSSHGESRTIRATYP 116
           MADSGTQYDVIVVGAGVMGSSTAYHLAKTGNKVLILEQFDFLHHRGSSHGESRTIRATYP
Sbjct: 1   MADSGTQYDVIVVGAGVMGSSTAYHLAKTGNKVLILEQFDFLHHRGSSHGESRTIRATYP 60

Query: 117 EDYYHSLVMESYELWRTAEAEIGYRVYFPAEQLDIGHSDDKSLAAAVDTCKKHSIPHLVL 176
           EDYYHSLVMESYELWRTAEAEIGYRVYFPAEQLDIGHSDDKSLAAAVDTCKKHSIPHLVL
Sbjct: 61  EDYYHSLVMESYELWRTAEAEIGYRVYFPAEQLDIGHSDDKSLAAAVDTCKKHSIPHLVL 120

Query: 177 DREQLAEKYSGRVEIPADWVAVWSKYGGVIKPTKAVSMFQTLAFKNGADLKDNAEVVEIK 236
           DREQLAEKYSGRVEIPADWVAVWSKYGGVIKPTKAVSMFQTLAFKNGADLKDNAEVVEIK
Sbjct: 121 DREQLAEKYSGRVEIPADWVAVWSKYGGVIKPTKAVSMFQTLAFKNGADLKDNAEVVEIK 180

Query: 237 RDGSSGGVVVSVANGERFRGKKCVVTVGAWARKLVKSVGGIELPIQPLEATVSYWRIKEG 296
           RDGSSGGVVVSVANGERFRGKKCVVTVGAWARKLVKSVGGIELPIQPLEATVSYWRIKEG
Sbjct: 181 RDGSSGGVVVSVANGERFRGKKCVVTVGAWARKLVKSVGGIELPIQPLEATVSYWRIKEG 240

Query: 297 AEGEYAIGGNFPTFASYGDTYIYGTPSLEFPGLIKVAVHGGHRCDPDKRSWGSGGQLPIT 356
           AEGEYAIGGNFPTFASYGDTYIYGTPSLEFPGLIKVAVHGGHRCDPDKRSWGSGGQLPIT
Sbjct: 241 AEGEYAIGGNFPTFASYGDTYIYGTPSLEFPGLIKVAVHGGHRCDPDKRSWGSGGQLPIT 300

Query: 357 ALKKWIEGRFGGRVDSSEPVSTQLCMYSMTPDEDFVIDFLGGEFGKDVVIGGGFSGHGFK 416
           ALKKWIEGRFGGRVDSSEPVSTQLCMYSMTPDEDFVIDFLGGEFGKDVVIGGGFSGHGFK
Sbjct: 301 ALKKWIEGRFGGRVDSSEPVSTQLCMYSMTPDEDFVIDFLGGEFGKDVVIGGGFSGHGFK 360

Query: 417 MSPAIGRILADLALKGVAEGLELKYFRIARFEENPKGNVKGFADQVRLH 466
           MSPAIGRILADLALKGVAEGLELKYFRIARFEENPKGNVKGFADQVRLH
Sbjct: 361 MSPAIGRILADLALKGVAEGLELKYFRIARFEENPKGNVKGFADQVRLH 409

[Close]

BLAST of CmaCh09G005320 vs. NCBI nr
Match: KAG7024517.1 (putative sarcosine oxidase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 828.9 bits (2140), Expect = 2.1e-236
Identity = 401/409 (98.04%), Postives = 408/409 (99.76%), Query Frame = 0


Query: 57  MADSGTQYDVIVVGAGVMGSSTAYHLAKTGNKVLILEQFDFLHHRGSSHGESRTIRATYP 116
           MADSGTQYDVIVVGAGVMGSSTAYHLAKTGNKVLILEQFDFLHHRGSSHGESRTIRATYP
Sbjct: 1   MADSGTQYDVIVVGAGVMGSSTAYHLAKTGNKVLILEQFDFLHHRGSSHGESRTIRATYP 60

Query: 117 EDYYHSLVMESYELWRTAEAEIGYRVYFPAEQLDIGHSDDKSLAAAVDTCKKHSIPHLVL 176
           EDYYHSLVMESYELW+TAEAEIGYRVYFPAEQLDIGHSDDKSLAAAVDTCKKHSIPHLVL
Sbjct: 61  EDYYHSLVMESYELWQTAEAEIGYRVYFPAEQLDIGHSDDKSLAAAVDTCKKHSIPHLVL 120

Query: 177 DREQLAEKYSGRVEIPADWVAVWSKYGGVIKPTKAVSMFQTLAFKNGADLKDNAEVVEIK 236
           DREQLAEKYSGRVEIPADWVAVWSKYGGVIKPTKAVSMFQTLAF+NGADLKDNAEVVEIK
Sbjct: 121 DREQLAEKYSGRVEIPADWVAVWSKYGGVIKPTKAVSMFQTLAFRNGADLKDNAEVVEIK 180

Query: 237 RDGSSGGVVVSVANGERFRGKKCVVTVGAWARKLVKSVGGIELPIQPLEATVSYWRIKEG 296
           RDGSSGGVVVS+ANGERFRGKKCVVTVGAWARKLVKSVGGIELPIQPLEATVSYWRIKEG
Sbjct: 181 RDGSSGGVVVSIANGERFRGKKCVVTVGAWARKLVKSVGGIELPIQPLEATVSYWRIKEG 240

Query: 297 AEGEYAIGGNFPTFASYGDTYIYGTPSLEFPGLIKVAVHGGHRCDPDKRSWGSGGQLPIT 356
           AEGEYAIGG+FPTFASYGDTYIYGTPSLEFPGLIKVAVHGGHRCDP+KRSWGSGG+LPIT
Sbjct: 241 AEGEYAIGGDFPTFASYGDTYIYGTPSLEFPGLIKVAVHGGHRCDPNKRSWGSGGELPIT 300

Query: 357 ALKKWIEGRFGGRVDSSEPVSTQLCMYSMTPDEDFVIDFLGGEFGKDVVIGGGFSGHGFK 416
           ALK+WIEGRFGGRVDSSEPVSTQLCMYSMTPDEDFVIDFLGGEFGKDVVIGGGFSGHGFK
Sbjct: 301 ALKEWIEGRFGGRVDSSEPVSTQLCMYSMTPDEDFVIDFLGGEFGKDVVIGGGFSGHGFK 360

Query: 417 MSPAIGRILADLALKGVAEGLELKYFRIARFEENPKGNVKGFADQVRLH 466
           MSPAIGRILADLALKGVAEGLELKYFRIARFEENPKGNVK FADQVRLH
Sbjct: 361 MSPAIGRILADLALKGVAEGLELKYFRIARFEENPKGNVKSFADQVRLH 409

[Close]

BLAST of CmaCh09G005320 vs. NCBI nr
Match: XP_022936028.1 (probable sarcosine oxidase [Cucurbita moschata])

HSP 1 Score: 828.9 bits (2140), Expect = 2.1e-236
Identity = 402/409 (98.29%), Postives = 406/409 (99.27%), Query Frame = 0


Query: 57  MADSGTQYDVIVVGAGVMGSSTAYHLAKTGNKVLILEQFDFLHHRGSSHGESRTIRATYP 116
           MADSGTQYDVIVVGAGVMGSSTAYHLAKTGNKVL LEQFDFLHHRGSSHGESRTIRATYP
Sbjct: 1   MADSGTQYDVIVVGAGVMGSSTAYHLAKTGNKVLTLEQFDFLHHRGSSHGESRTIRATYP 60

Query: 117 EDYYHSLVMESYELWRTAEAEIGYRVYFPAEQLDIGHSDDKSLAAAVDTCKKHSIPHLVL 176
           EDYYHSLVMESYELWRTAEAEIGYRVYFPAEQLDIGHSDDKSLAAAVDTCKKHSIPHLVL
Sbjct: 61  EDYYHSLVMESYELWRTAEAEIGYRVYFPAEQLDIGHSDDKSLAAAVDTCKKHSIPHLVL 120

Query: 177 DREQLAEKYSGRVEIPADWVAVWSKYGGVIKPTKAVSMFQTLAFKNGADLKDNAEVVEIK 236
           DREQLAEKYSGRVEIPADWVAVWSKYGGVIKPTKAVSMFQTLAFKNGADLKDNAEVVEIK
Sbjct: 121 DREQLAEKYSGRVEIPADWVAVWSKYGGVIKPTKAVSMFQTLAFKNGADLKDNAEVVEIK 180

Query: 237 RDGSSGGVVVSVANGERFRGKKCVVTVGAWARKLVKSVGGIELPIQPLEATVSYWRIKEG 296
           R+GSSGGVVVS ANGERFRGKKCVVTVGAWARKLVKSVGGIELPIQPLEATVSYWRIKEG
Sbjct: 181 REGSSGGVVVSTANGERFRGKKCVVTVGAWARKLVKSVGGIELPIQPLEATVSYWRIKEG 240

Query: 297 AEGEYAIGGNFPTFASYGDTYIYGTPSLEFPGLIKVAVHGGHRCDPDKRSWGSGGQLPIT 356
           AEGEYAIGG+FPTFASYGDTYIYGTPSLEFPGLIKVAVHGGHRCDPDKRSWGSGG+LPIT
Sbjct: 241 AEGEYAIGGDFPTFASYGDTYIYGTPSLEFPGLIKVAVHGGHRCDPDKRSWGSGGELPIT 300

Query: 357 ALKKWIEGRFGGRVDSSEPVSTQLCMYSMTPDEDFVIDFLGGEFGKDVVIGGGFSGHGFK 416
           ALK+WIEGRFGGRVDSSEPVSTQLCMYSMTPDEDFVIDFLGGEFGKDVVIGGGFSGHGFK
Sbjct: 301 ALKEWIEGRFGGRVDSSEPVSTQLCMYSMTPDEDFVIDFLGGEFGKDVVIGGGFSGHGFK 360

Query: 417 MSPAIGRILADLALKGVAEGLELKYFRIARFEENPKGNVKGFADQVRLH 466
           MSPAIGRILADLALKGVAEGLELKYFRIARFEENPKGNVK FADQVRLH
Sbjct: 361 MSPAIGRILADLALKGVAEGLELKYFRIARFEENPKGNVKSFADQVRLH 409

[Close]

BLAST of CmaCh09G005320 vs. NCBI nr
Match: XP_023534889.1 (probable sarcosine oxidase [Cucurbita pepo subsp. pepo])

HSP 1 Score: 827.4 bits (2136), Expect = 6.1e-236
Identity = 401/409 (98.04%), Postives = 406/409 (99.27%), Query Frame = 0


Query: 57  MADSGTQYDVIVVGAGVMGSSTAYHLAKTGNKVLILEQFDFLHHRGSSHGESRTIRATYP 116
           MADSGTQYDVIVVGAGVMGSSTAYHLAKTGNKVLILEQFDFLHHRGSSHGESRTIRATYP
Sbjct: 1   MADSGTQYDVIVVGAGVMGSSTAYHLAKTGNKVLILEQFDFLHHRGSSHGESRTIRATYP 60

Query: 117 EDYYHSLVMESYELWRTAEAEIGYRVYFPAEQLDIGHSDDKSLAAAVDTCKKHSIPHLVL 176
           EDYYHSLVMESYELWRTAEAEIGYRVYFPAEQLDIGHSDDKSLAAAVDTCKKHSIPHLVL
Sbjct: 61  EDYYHSLVMESYELWRTAEAEIGYRVYFPAEQLDIGHSDDKSLAAAVDTCKKHSIPHLVL 120

Query: 177 DREQLAEKYSGRVEIPADWVAVWSKYGGVIKPTKAVSMFQTLAFKNGADLKDNAEVVEIK 236
           DREQLAEKY GRVEIPADWVAVWSKYGGVIKPTKAVSMFQTLAFKNGADLKDNAEVVEIK
Sbjct: 121 DREQLAEKYYGRVEIPADWVAVWSKYGGVIKPTKAVSMFQTLAFKNGADLKDNAEVVEIK 180

Query: 237 RDGSSGGVVVSVANGERFRGKKCVVTVGAWARKLVKSVGGIELPIQPLEATVSYWRIKEG 296
           RDGSSGGVVVS+ANGERFRGKKCVVTVGAWARKLVKSVGGIELPIQPLEATVSYWRIKEG
Sbjct: 181 RDGSSGGVVVSIANGERFRGKKCVVTVGAWARKLVKSVGGIELPIQPLEATVSYWRIKEG 240

Query: 297 AEGEYAIGGNFPTFASYGDTYIYGTPSLEFPGLIKVAVHGGHRCDPDKRSWGSGGQLPIT 356
           AEGEYAIGG+FPTFASYGDTYIYGTPSLEFPGLIKVAVHGGHRCDPDKRSWGSGG+LPIT
Sbjct: 241 AEGEYAIGGDFPTFASYGDTYIYGTPSLEFPGLIKVAVHGGHRCDPDKRSWGSGGELPIT 300

Query: 357 ALKKWIEGRFGGRVDSSEPVSTQLCMYSMTPDEDFVIDFLGGEFGKDVVIGGGFSGHGFK 416
           ALK+WIEGRFGGRVDSSEPVSTQLCMYSMTPDEDFVIDFLGGEFGKDVVIGGGFSGHGFK
Sbjct: 301 ALKEWIEGRFGGRVDSSEPVSTQLCMYSMTPDEDFVIDFLGGEFGKDVVIGGGFSGHGFK 360

Query: 417 MSPAIGRILADLALKGVAEGLELKYFRIARFEENPKGNVKGFADQVRLH 466
           MSPAIGRILADLALKGVAEG+ELKYFRIARFE NPKGNVK FADQVRLH
Sbjct: 361 MSPAIGRILADLALKGVAEGMELKYFRIARFEGNPKGNVKDFADQVRLH 409

[Close]

BLAST of CmaCh09G005320 vs. NCBI nr
Match: KAG6591634.1 (putative sarcosine oxidase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 775.4 bits (2001), Expect = 2.8e-220
Identity = 380/409 (92.91%), Postives = 385/409 (94.13%), Query Frame = 0


Query: 57  MADSGTQYDVIVVGAGVMGSSTAYHLAKTGNKVLILEQFDFLHHRGSSHGESRTIRATYP 116
           MADSGTQYDVIVVGAGVMGSSTAYHLAKTGNKVLILEQFDFLHHRGSSHGESRTIRATYP
Sbjct: 1   MADSGTQYDVIVVGAGVMGSSTAYHLAKTGNKVLILEQFDFLHHRGSSHGESRTIRATYP 60

Query: 117 EDYYHSLVMESYELWRTAEAEIGYRVYFPAEQLDIGHSDDKSLAAAVDTCKKHSIPHLVL 176
           EDYYHSLVMESYELW+TAEAEIGYRVYFPAEQLDIGHSDDKSLAAAVDTCKKHSIPHLVL
Sbjct: 61  EDYYHSLVMESYELWQTAEAEIGYRVYFPAEQLDIGHSDDKSLAAAVDTCKKHSIPHLVL 120

Query: 177 DREQLAEKYSGRVEIPADWVAVWSKYGGVIKPTKAVSMFQTLAFKNGADLKDNAEVVEIK 236
           DREQLAEKYSGRVEIPADWVAVWSKYGGVIKPTKAVSMFQTLAFKNGADLKDNAEVVEIK
Sbjct: 121 DREQLAEKYSGRVEIPADWVAVWSKYGGVIKPTKAVSMFQTLAFKNGADLKDNAEVVEIK 180

Query: 237 RDGSSGGVVVSVANGERFRGKKCVVTVGAWARKLVKSVGGIELPIQPLEATVSYWRIKEG 296
           RDG                       +GAWARKLVKSVGGIELPIQPLEATVSYWRIKEG
Sbjct: 181 RDG-----------------------IGAWARKLVKSVGGIELPIQPLEATVSYWRIKEG 240

Query: 297 AEGEYAIGGNFPTFASYGDTYIYGTPSLEFPGLIKVAVHGGHRCDPDKRSWGSGGQLPIT 356
           AEGEYAIGG+FPTFASYGDTYIYGTPSLEFPGLIKVAVHGGHRCDPDKRSWGSGG+LPIT
Sbjct: 241 AEGEYAIGGDFPTFASYGDTYIYGTPSLEFPGLIKVAVHGGHRCDPDKRSWGSGGELPIT 300

Query: 357 ALKKWIEGRFGGRVDSSEPVSTQLCMYSMTPDEDFVIDFLGGEFGKDVVIGGGFSGHGFK 416
           ALK+WIEGRFGGRVDSSEPVSTQLCMYSMTPDEDFVIDFLGGEFGKDVVIGGGFSGHGFK
Sbjct: 301 ALKEWIEGRFGGRVDSSEPVSTQLCMYSMTPDEDFVIDFLGGEFGKDVVIGGGFSGHGFK 360

Query: 417 MSPAIGRILADLALKGVAEGLELKYFRIARFEENPKGNVKGFADQVRLH 466
           MSPAIGRILADLALKGVAEGLELKYFRIARFEENPKGNVK FADQVRLH
Sbjct: 361 MSPAIGRILADLALKGVAEGLELKYFRIARFEENPKGNVKSFADQVRLH 386

[Close]

BLAST of CmaCh09G005320 vs. TAIR 10
Match: AT2G24580.1 (FAD-dependent oxidoreductase family protein )

HSP 1 Score: 568.9 bits (1465), Expect = 3.7e-162
Identity = 269/412 (65.29%), Postives = 329/412 (79.85%), Query Frame = 0


Query: 56  EMADSGTQYDVIVVGAGVMGSSTAYHLAKTGNKVLILEQFDFLHHRGSSHGESRTIRATY 115
           E +D G ++DVIVVGAGVMGSS AY LAK G K L+LEQFDFLHHRGSSHGESRTIRATY
Sbjct: 2   EYSDDG-RFDVIVVGAGVMGSSAAYQLAKRGQKTLLLEQFDFLHHRGSSHGESRTIRATY 61

Query: 116 PEDYYHSLVMESYELWRTAEAEIGYRVYFPAEQLDIGHSDDKSLAAAVDTCKKHSIPHLV 175
           PEDYY+S+V ES  LW  A++EIGY+V+FP +Q D+G +D +SL + V TC+KH + H V
Sbjct: 62  PEDYYYSMVSESTRLWAAAQSEIGYKVHFPTQQFDMGPADQQSLLSVVATCQKHGLAHRV 121

Query: 176 LDREQLAEKYSGRVEIPADWVAVWSKYGGVIKPTKAVSMFQTLAFKNGADLKDNAEVVEI 235
           +D   ++E +SGR+ IP +W+ V ++ GG+IKPTKAVSMFQTLA  +GA L+DN +V  I
Sbjct: 122 MDSHAVSEHFSGRISIPENWIGVSTELGGIIKPTKAVSMFQTLAIGHGAILRDNTKVANI 181

Query: 236 KRDGSSG-GVVVSVANGERFRGKKCVVTVGAWARKLVKSVGGIELPIQPLEATVSYWRIK 295
           KRDG SG GV+V    G++F GKKC+VT GAW  KLVK+V GI+ P++PLE TV YWRIK
Sbjct: 182 KRDGESGEGVIVCTVKGDKFYGKKCIVTAGAWISKLVKTVAGIDFPVEPLETTVCYWRIK 241

Query: 296 EGAEGEYAIGGNFPTFASYGDTYIYGTPSLEFPGLIKVAVHGGHRCDPDKRSWGSGGQLP 355
           EG E ++ I G FPTFASYG  Y+YGTPSLE+PGLIKVAVHGG+ CDPDKR WG G  + 
Sbjct: 242 EGHEEKFTIDGEFPTFASYGAPYVYGTPSLEYPGLIKVAVHGGYWCDPDKRPWGPG--VK 301

Query: 356 ITALKKWIEGRFGGRVDSSEPVSTQLCMYSMTPDEDFVIDFLGGEFGKDVVIGGGFSGHG 415
           +  LK+WI+ RFGG VDS  PV+TQLCMYSMTPDEDFVIDFLGGEFG+DVV+GGGFSGHG
Sbjct: 302 LEELKEWIKERFGGMVDSEGPVATQLCMYSMTPDEDFVIDFLGGEFGRDVVVGGGFSGHG 361

Query: 416 FKMSPAIGRILADLALKGVA--EGLELKYFRIARFEENPKGNVKGFADQVRL 465
           FKM+PA+GRILAD+A++  A   G+E+K F + RFE+NPKGN K + DQV L
Sbjct: 362 FKMAPAVGRILADMAMEVEAGGGGVEMKQFSLRRFEDNPKGNAKEYPDQVIL 410

[Close]

BLAST of CmaCh09G005320 vs. TAIR 10
Match: AT5G24155.1 (FAD/NAD(P)-binding oxidoreductase family protein )

HSP 1 Score: 43.9 bits (102), Expect = 4.1e-04
Identity = 26/53 (49.06%), Postives = 34/53 (64.15%), Query Frame = 0


Query: 45 RKKSSKQI---GGEEMADSGTQYDVIVVGAGVMGSSTAYHLAKTGNKVLILEQ 95
          RKK + ++     EE  DS    DVI+VGAGV GS+ AY LAK G +VL +E+
Sbjct: 28 RKKKAPELADAAAEERRDSAA--DVIIVGAGVGGSALAYSLAKDGRRVLAIER 78

The following BLAST results are available for this feature:

BLAST of CmaCh09G005320 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SJA7	5.2e-161	65.29	Probable sarcosine oxidase OS=Arabidopsis thaliana OX=3702 GN=At2g24580 PE=2 SV=...	[more]
Q29RU9	4.5e-64	36.30	Peroxisomal sarcosine oxidase OS=Bos taurus OX=9913 GN=PIPOX PE=2 SV=2	[more]
Q9P0Z9	3.2e-62	35.47	Peroxisomal sarcosine oxidase OS=Homo sapiens OX=9606 GN=PIPOX PE=1 SV=2	[more]
P79371	2.7e-61	34.98	Peroxisomal sarcosine oxidase OS=Oryctolagus cuniculus OX=9986 GN=PIPOX PE=1 SV=...	[more]
Q9D826	1.1e-59	34.90	Peroxisomal sarcosine oxidase OS=Mus musculus OX=10090 GN=Pipox PE=1 SV=1	[more]

BLAST of CmaCh09G005320 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1IIQ6	2.6e-240	100.00	Sarcosine oxidase OS=Cucurbita maxima OX=3661 GN=LOC111474391 PE=3 SV=1	[more]
A0A6J1FC48	1.0e-236	98.29	Sarcosine oxidase OS=Cucurbita moschata OX=3662 GN=LOC111442750 PE=3 SV=1	[more]
A0A0A0LJG6	1.8e-204	83.86	Sarcosine oxidase OS=Cucumis sativus OX=3659 GN=Csa_3G881890 PE=3 SV=1	[more]
A0A5D3E5F8	3.1e-201	83.37	Sarcosine oxidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold455G00...	[more]
A0A1S3CR44	4.1e-201	83.37	Sarcosine oxidase OS=Cucumis melo OX=3656 GN=LOC103503750 PE=3 SV=1	[more]

BLAST of CmaCh09G005320 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022975268.1	5.4e-240	100.00	probable sarcosine oxidase [Cucurbita maxima]	[more]
KAG7024517.1	2.1e-236	98.04	putative sarcosine oxidase, partial [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_022936028.1	2.1e-236	98.29	probable sarcosine oxidase [Cucurbita moschata]	[more]
XP_023534889.1	6.1e-236	98.04	probable sarcosine oxidase [Cucurbita pepo subsp. pepo]	[more]
KAG6591634.1	2.8e-220	92.91	putative sarcosine oxidase, partial [Cucurbita argyrosperma subsp. sororia]	[more]

BLAST of CmaCh09G005320 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G24580.1	3.7e-162	65.29	FAD-dependent oxidoreductase family protein	[more]
AT5G24155.1	4.1e-04	49.06	FAD/NAD(P)-binding oxidoreductase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006281	Sarcosine oxidase, monomeric	TIGRFAM	TIGR01377	TIGR01377	coord: 64..453 e-value: 7.1E-151 score: 500.2
IPR006076	FAD dependent oxidoreductase	PFAM	PF01266	DAO	coord: 65..428 e-value: 3.3E-49 score: 168.4
None	No IPR available	GENE3D	3.30.9.10		coord: 147..385 e-value: 1.1E-103 score: 349.5
None	No IPR available	PANTHER	PTHR10961:SF7	PEROXISOMAL SARCOSINE OXIDASE	coord: 62..457
None	No IPR available	PANTHER	PTHR10961	PEROXISOMAL SARCOSINE OXIDASE	coord: 62..457
None	No IPR available	SUPERFAMILY	54373	FAD-linked reductases, C-terminal domain	coord: 282..387
IPR036188	FAD/NAD(P)-binding domain superfamily	GENE3D	3.50.50.60		coord: 64..432 e-value: 1.1E-103 score: 349.5
IPR036188	FAD/NAD(P)-binding domain superfamily	SUPERFAMILY	51905	FAD/NAD(P)-binding domain	coord: 63..448

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmaCh09G005320.1	CmaCh09G005320.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0046653	tetrahydrofolate metabolic process
molecular_function	GO:0008115	sarcosine oxidase activity
molecular_function	GO:0016491	oxidoreductase activity