CmaCh09G004560 (gene) Cucurbita maxima (Rimu) v1.1

Overview


Name	CmaCh09G004560
Type	gene
Organism	Cucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Description	ENTH domain-containing protein
Location	Cma_Chr09: 1997480 .. 1999937 (+)
RNA-Seq Expression	CmaCh09G004560
Synteny	CmaCh09G004560

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

CCTATTTTTTGGCCACCGACGGCCAAAAATCTACGAACCGAGGAGATCGTCGGGAATGTACGGTGGCTCTACGCTGCCATTCCGGCGTCTTTTTCGCTTCATTATCTTTCACTCACTGAACCACTGTTTTCAGCTCGCTTTTAAACTCCAGCGATTGAAGAATATTGTTTTTGCTTATTCGAGGCCGTTAACGACCGATCGGATTCTTATCGTGCAGGTAAGTTTGAAAACTTAAAAGTGTTTTTTGTTTCTCCTTATGGACTGTTCTCAGTTTTTTTTTAATATGTGATTGCTTAGTGCTAGTTTTTAGCCAATGATTCGTGGATTTGAACGAGCACAATGATATATGATGATTTTTCTGTATTTTTCTCTTTTTTCTGTGTTCGTTGAATATTTAGCTTGGGAGGGACTATATCGTGATTGAAGTAGTGAGTTTGAGAGAGCTGTATCGACATAGAACTGTAGAATAGTAGGAAGATTTTGACATAGAATAGTAGGATGATTTTGGTGCCGGCCAACTGGTTTGACATAGAATAGTAGGATGATTTTGATGCGCTGTGCCCTCTAGTGAAGGAGAGTTTTCAGTTGTATTCGGATATTTGTGAAGTCTTGGCTCTTTTGCATGATACGTTCTTTGGTATGTAGCATCCGGACTGTGTCAAGGCATTTGATGCGTATGATATTGCAGGGAAGCAGATTGATGAGCTCTCTATGTTTTATAAATTGGTGTAAAGATGTAGGTGTTGTAAGGTCTTCTGAGTATCTAGAGGTTCGGGGAATTGACTGCAAGGTATTGGAGACATTGGATACGTTTGTTAGGGACAGAGCAAAAAGACCGACGAATCGAGAGAAGGAGCAACGTTTACCTTCTTGAGGATTATTGAGAGGAGTACTCCCCTTGGTTAATTAAGAGGAAGATCATGAGTTTATGACTAAGGAACACTATCTCCATTGGTATCACGTCTTTAGAGGAAACCAAAACAAACCAAAAGCAAAGTCATGAGAGCTTGCTTATGAAAGTGAACAATACCGTACCATTGTGGAGGTTCTTGATTCTTACTATGGTATCGTAACTATGTCTTTTACTTAGTCATGCCAATAATATCTTCAAGTGTCGAACGAAAAAGTTGTGAGCCTACCCCTTATTCAAGGGCTCTAGAGAAAGAGTTGAGTCTCGATTAAGAGGGGGCTATTCGATAGCAGCTCTGGAGAAGGAGGTCTCAAGGGAGGCTCTATGGTGTACTTTTTTCGAGAAAAAGATTGTCGGGAATTATTGAGCCTTTACAAATTAAGAGGAATATCATGAACTAATTAAGAGGTTTCTGATTCCTGCTTTACCTCCGAAGAGCCAATGACTGATATGAATGAAATAAAAGCTCTATCACTCCACCTGAAAATCATACTTGAACCTCCTCCTGTGCCCAAACCTCAACAAAAATTCGTAGAAGACTTGGGTCAATTTGAGAGACGATGCTGTTAGTTCAGATGATCAAGGTCATAGTCGTCTGGCACTGGCTTTGTTTGCTGGCTTAGCAGCTAATGGCACAAATGAATCCTGGGAAGCTTTCCCTTCTTCCAATCGACAGCCAGAAGTAACCCATGGCAGGGCAGACGCCTGCTGCTGGACCTGGGAAAGCGGATTGGGAACTGGCCTTGATATAGATGCAAGCAATTTAACGTGGGAGAGAGCAGCACTTGGTGGTGGATTTGATCCATTATTGTTAAATGGCATGTATGATCAAAGAATAGTTAGACTGCATACCAGCACTGCCCAATTAAGTGGTGGAAGTGCTAGCAGTCTACCAACAACAAAACTCCTGATTTGGCTACCATTCAAACGGTTAATCAGGATCCTTTCGCAGCATCGTAAAGCATCCCGCCTCCGTCATATGTGCAAATGGCCGATATGGAGAAGAAACTACAACTTCTAGTGAGCAACAACAAATATAATAGCATTATACAAGAGACGAGTGTTAGGAACTACAAACCTCCATAATGATATGATATTGTCTATTTTGAACATAAGTTATCGTAGCTTTGCTTTTTGTTTAATCAAAATGCTTAATAAAAGATGCAGTGCCAAAATCAGTAACCACTCGAGTTACTATGCTGCAAGACCAGTGATTCAGAGTGCCCTATTGAATGCCTCTGGTTAGGAGTGAGTGGGTATTACTACACTCAGTGATGCCTACCTTTGCCTTGCATCTCTACTCTTTTACTCGGTTCGTTGTGGCTATAGCTCTATTCAATTTCTAAACTTTCATTAGGCCTATAATTAGAAATCAATTGTTTTCTTGGCGATAAATTTAATTTTTATGTGTAATATAATTTTAAGCTTTTAAATTAAATTTATAATTTAAAAAGTTTACACGGGTATGTATAGTATCATTCAACTAATCCATAATTATTTGCTTCATAAAGTTTTTTTTTTTTTTTTTTTTGAAGATCCAGGCATTGA

mRNA sequence

CCTATTTTTTGGCCACCGACGGCCAAAAATCTACGAACCGAGGAGATCGTCGGGAATGTACGGTGGCTCTACGCTGCCATTCCGGCGTCTTTTTCGCTTCATTATCTTTCACTCACTGAACCACTGTTTTCAGCTCGCTTTTAAACTCCAGCGATTGAAGAATATTGTTTTTGCTTATTCGAGGCCGTTAACGACCGATCGGATTCTTATCGTGCAGGATGATTTTGATGCGCTGTGCCCTCTAGTGAAGGAGAGTTTTCAGTTGTATTCGGATATTTGTGAAGTCTTGGCTCTTTTGCATGATACCATCCGGACTGTGTCAAGGCATTTGATGCGTATGATATTGCAGGGAAGCAGATTGATGAGCTCTCTATGTTTTATAAATTGGTGTAAAGATGTAGGTGTTGTAAGGTCTTCTGAGTATCTAGAGGTTCGGGGAATTGACTGCAAGGTATTGGAGACATTGGATACTCGTCTGGCACTGGCTTTGTTTGCTGGCTTAGCAGCTAATGGCACAAATGAATCCTGGGAAGCTTTCCCTTCTTCCAATCGACAGCCAGAAGTAACCCATGGCAGGGCAGACGCCTGCTGCTGGACCTGGGAAAGCGGATTGGGAACTGGCCTTGATATAGATGCAAGCAATTTAACGTGGGAGAGAGCAGCACTTGGTGGTGGATTTGATCCATTATTGTTAAATGGCATGTATGATCAAAGAATAGTTAGACTGCATACCAGCACTGCCCAATTAAGTGGTGGAAGTGCTAGCAGTCTACCAACAACAAAACTCCTGATTTGGCTACCATTCAAACGATCCAGGCATTGA

Coding sequence (CDS)

ATGTACGGTGGCTCTACGCTGCCATTCCGGCGTCTTTTTCGCTTCATTATCTTTCACTCACTGAACCACTGTTTTCAGCTCGCTTTTAAACTCCAGCGATTGAAGAATATTGTTTTTGCTTATTCGAGGCCGTTAACGACCGATCGGATTCTTATCGTGCAGGATGATTTTGATGCGCTGTGCCCTCTAGTGAAGGAGAGTTTTCAGTTGTATTCGGATATTTGTGAAGTCTTGGCTCTTTTGCATGATACCATCCGGACTGTGTCAAGGCATTTGATGCGTATGATATTGCAGGGAAGCAGATTGATGAGCTCTCTATGTTTTATAAATTGGTGTAAAGATGTAGGTGTTGTAAGGTCTTCTGAGTATCTAGAGGTTCGGGGAATTGACTGCAAGGTATTGGAGACATTGGATACTCGTCTGGCACTGGCTTTGTTTGCTGGCTTAGCAGCTAATGGCACAAATGAATCCTGGGAAGCTTTCCCTTCTTCCAATCGACAGCCAGAAGTAACCCATGGCAGGGCAGACGCCTGCTGCTGGACCTGGGAAAGCGGATTGGGAACTGGCCTTGATATAGATGCAAGCAATTTAACGTGGGAGAGAGCAGCACTTGGTGGTGGATTTGATCCATTATTGTTAAATGGCATGTATGATCAAAGAATAGTTAGACTGCATACCAGCACTGCCCAATTAAGTGGTGGAAGTGCTAGCAGTCTACCAACAACAAAACTCCTGATTTGGCTACCATTCAAACGATCCAGGCATTGA

Protein sequence

MYGGSTLPFRRLFRFIIFHSLNHCFQLAFKLQRLKNIVFAYSRPLTTDRILIVQDDFDALCPLVKESFQLYSDICEVLALLHDTIRTVSRHLMRMILQGSRLMSSLCFINWCKDVGVVRSSEYLEVRGIDCKVLETLDTRLALALFAGLAANGTNESWEAFPSSNRQPEVTHGRADACCWTWESGLGTGLDIDASNLTWERAALGGGFDPLLLNGMYDQRIVRLHTSTAQLSGGSASSLPTTKLLIWLPFKRSRH

Homology

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BLAST of CmaCh09G004560 vs. ExPASy Swiss-Prot
Match: Q8S9J8 (Probable clathrin assembly protein At4g32285 OS=Arabidopsis thaliana OX=3702 GN=At4g32285 PE=1 SV=2)

HSP 1 Score: 118.2 bits (295), Expect = 1.3e-25
Identity = 106/330 (32.12%), Postives = 138/330 (41.82%), Query Frame = 0


Query: 5   STLPFRRLFRFIIFHSLNHCFQLAFKLQRLKNIVFAYSRP---LTTDRILIVQDDFDALC 64
           S  P R +    IF  + H       LQRL +  F   RP       R++++     A+ 
Sbjct: 222 SVTPLREMTPERIFGKMGH-------LQRLLD-RFLSCRPTGLAKNSRMILI-----AMY 281

Query: 65  PLVKESFQLYSDICEVLALLHDTIRTVS-----RHLMRMILQGSRLMSSLCFINWCKDVG 124
           P+VKESF+LY+DICEVLA+L D    +      +          ++   + F +WCKD G
Sbjct: 282 PVVKESFRLYADICEVLAVLLDKFFDMEYTDCVKAFDAYASAAKQIDELIAFYHWCKDTG 341

Query: 125 VVRSSEYLEVRGIDCKVLETLD-------------------------------------- 184
           V RSSEY EV+ I  K+LETL+                                      
Sbjct: 342 VARSSEYPEVQRITSKLLETLEEFVRDRAKRAKSPERKEIEAPPAPAPPVEEPVDMNEIK 401

Query: 185 -------------------------------------------TRLALALFAGLAANGTN 240
                                                       + ALALFAG  AN  N
Sbjct: 402 ALPPPENHTPPPPPAPEPKPQQPQVTDDLVNLREDDVSGDDQGNKFALALFAGPPAN--N 461

[Close]

BLAST of CmaCh09G004560 vs. ExPASy Swiss-Prot
Match: Q8LF20 (Putative clathrin assembly protein At2g25430 OS=Arabidopsis thaliana OX=3702 GN=At2g25430 PE=1 SV=2)

HSP 1 Score: 113.6 bits (283), Expect = 3.3e-24
Identity = 101/323 (31.27%), Postives = 133/323 (41.18%), Query Frame = 0


Query: 8   PFRRLFRFIIFHSLNHCFQLAFKLQRLKNIVFAYSRPLTTDRILIVQDDFDALCPLVKES 67
           P R +    IF  + H  +L  +   L+    A        R++++     AL P+V+ES
Sbjct: 245 PLREMTPERIFGKMGHLQRLLDRFLSLRPTGLA-----KNSRMILI-----ALYPVVRES 304

Query: 68  FQLYSDICEVLALLHDTIRTVS-----RHLMRMILQGSRLMSSLCFINWCKDVGVVRSSE 127
           F+LY+DICEVLA+L D    +      +          ++   + F NWCK+ GV RSSE
Sbjct: 305 FKLYADICEVLAVLLDKFFDMEYSDCVKAFDAYASAAKQIDELIAFYNWCKETGVARSSE 364

Query: 128 YLEVRGIDCKVLETLD-------------------------------------------- 187
           Y EV+ I  K+LETL+                                            
Sbjct: 365 YPEVQRITSKLLETLEEFVRDRAKRGKSPERKEIEAPPPVVEEEEPEPDMNEIKALPPPE 424

Query: 188 -------------------------------------TRLALALFAGLAANGTNESWEAF 240
                                                 + ALALFAG    G N  WEAF
Sbjct: 425 NYTPPPPPEPEPQPEKPQFTEDLVNLREDEVTADDQGNKFALALFAG--PPGNNGKWEAF 484

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BLAST of CmaCh09G004560 vs. ExPASy Swiss-Prot
Match: Q9SA65 (Putative clathrin assembly protein At1g03050 OS=Arabidopsis thaliana OX=3702 GN=At1g03050 PE=2 SV=1)

HSP 1 Score: 70.5 bits (171), Expect = 3.2e-11
Identity = 85/325 (26.15%), Postives = 124/325 (38.15%), Query Frame = 0


Query: 26  QLAFKLQRLKNIV--FAYSRP---LTTDRILIVQDDFDALCPLVKESFQLYSDICEVLAL 85
           Q+  ++Q L+ ++  F   RP      +R++IV     AL P+VKESFQ+Y D+ E++ +
Sbjct: 202 QIFIRIQHLQQLLDRFLACRPTGNARNNRVVIV-----ALYPIVKESFQIYYDVTEIMGI 261

Query: 86  LHDTIRTV----SRHLMRMILQGSRLMSSL-CFINWCKDVGVVRSSEYLEVRGIDCKVLE 145
           L +    +    S  +  +  + S+    L  F +WCK++G+ RSSEY E+  I  K L+
Sbjct: 262 LIERFMELDIPDSIKVYDIFCRVSKQFEELDQFYSWCKNMGIARSSEYPEIEKITQKKLD 321

Query: 146 TLD--------------------------------------------------------- 205
            +D                                                         
Sbjct: 322 LMDEFIRDKSALEHTKQSKSVKSEADEDDDEARTEEVNEEQEDMNAIKALPEPPPKEEDD 381

Query: 206 --------------------------------------TRLALALFAGLAANGTNE---- 240
                                                   LALALF G  A+G+      
Sbjct: 382 VKPEEEAKEEVIIEKKQEEMGDLLDLGNTNGGEAGQAGDSLALALFDGPYASGSGSESGP 441

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BLAST of CmaCh09G004560 vs. ExPASy Swiss-Prot
Match: Q8GX47 (Putative clathrin assembly protein At4g02650 OS=Arabidopsis thaliana OX=3702 GN=At4g02650 PE=2 SV=2)

HSP 1 Score: 60.8 bits (146), Expect = 2.5e-08
Identity = 81/320 (25.31%), Postives = 119/320 (37.19%), Query Frame = 0


Query: 30  KLQRLKNIV--FAYSRP---LTTDRILIVQDDFDALCPLVKESFQLYSDICEVLALLHDT 89
           ++Q L+ ++  F   RP      +R++IV     A+ P+VKESFQLY +I E++ +L + 
Sbjct: 211 RVQHLQQLLDRFLACRPTGNAKNNRVVIV-----AMYPIVKESFQLYYNITEIMGVLIER 270

Query: 90  IRTVSRH----LMRMILQGSRLMSSL-CFINWCKDVGVVRSSEYLEVRGIDCKVLETLD- 149
              +  H    +  +  + S+    L  F  WCK++ V RSSEY E+  I  K L+ +D 
Sbjct: 271 FMELDIHDSIKVYEIFCRVSKQFDELDPFYGWCKNMAVARSSEYPELEKITQKKLDLMDE 330

Query: 150 ------------------------------------------------------------ 209
                                                                       
Sbjct: 331 FIRDKSALAAQTTKSSSKRSNKSEEEESKTEYIQENQEDLNSIKALPAPEQKEEEEEEEK 390

Query: 210 -----------------------------------TRLALALFAGLAA--NGTNESWEAF 240
                                                LALALF G+    + +   WEAF
Sbjct: 391 METKKDVEEVVSRQDQEGDLLDLTDEAGVTAGTVGDSLALALFDGVVGTESASGPGWEAF 450

[Close]

BLAST of CmaCh09G004560 vs. ExPASy TrEMBL
Match: A0A6J1IIW0 (uncharacterized protein LOC111476675 OS=Cucurbita maxima OX=3661 GN=LOC111476675 PE=4 SV=1)

HSP 1 Score: 174.5 bits (441), Expect = 5.7e-40
Identity = 95/139 (68.35%), Postives = 95/139 (68.35%), Query Frame = 0


Query: 1   MYGGSTLPFRRLFRFIIFHSLNHCFQLAFKLQRLKNIVFAYSRPLTTDRILIVQDDFDAL 60
           MYGGSTLPFRRLFRFIIFHSLNHCFQLAFKLQRLKNIVFAYSRPLTTDRILIV       
Sbjct: 1   MYGGSTLPFRRLFRFIIFHSLNHCFQLAFKLQRLKNIVFAYSRPLTTDRILIV------- 60

Query: 61  CPLVKESFQLYSDICEVLALLHDTIRTVSRHLMRMILQGSRLMSSLCFINWCKDVGVVRS 120
                                                QGSRLMSSLCFINWCKDVGVVRS
Sbjct: 61  -------------------------------------QGSRLMSSLCFINWCKDVGVVRS 95

Query: 121 SEYLEVRGIDCKVLETLDT 140
           SEYLEVRGIDCKVLETLDT
Sbjct: 121 SEYLEVRGIDCKVLETLDT 95

[Close]

BLAST of CmaCh09G004560 vs. ExPASy TrEMBL
Match: A0A6J1IEX9 (putative clathrin assembly protein At2g25430 OS=Cucurbita maxima OX=3661 GN=LOC111476610 PE=4 SV=1)

HSP 1 Score: 147.9 bits (372), Expect = 5.8e-32
Identity = 107/266 (40.23%), Postives = 125/266 (46.99%), Query Frame = 0


Query: 59  ALCPLVKESFQLYSDICEVLALLHDTI--RTVSRHLMRMILQGS---RLMSSLCFINWCK 118
           AL PLV+ESFQLY+DICEVLA+L D       S  +      GS   ++   + F NWCK
Sbjct: 275 ALYPLVRESFQLYADICEVLAVLLDKFFDMEYSDSMKAFDAYGSAAKQIDELIAFYNWCK 334

Query: 119 DVGVVRSSEYLEVRGIDCKVLETLD----------------------------------- 178
           D GV RSSEY EV+ I  K+LETL+                                   
Sbjct: 335 DTGVARSSEYPEVQRITSKLLETLEEFLRERGKAPKSPEREPPPPAPQEEEPVPDMNEIK 394

Query: 179 ---------------------------------------------TRLALALFAGLAANG 238
                                                         +LALALFAG AANG
Sbjct: 395 ALPPPENYTPPPPEPEPQPAPKPQPQVTEDLVNLRDDAVSADAQGNKLALALFAGPAANG 454

Query: 239 TNESWEAFPSSNRQPEVTHGRADACCWTWESGLGTGLDIDASNLTWERAALGGGFDPLLL 240
            N SWEAFPS   QPEVT           ++     L   ASNL+ ++AALGGG DPLLL
Sbjct: 455 ANVSWEAFPSDG-QPEVTSAWQTPAAEPGKADWELALVETASNLSRQKAALGGGLDPLLL 514

[Close]

BLAST of CmaCh09G004560 vs. ExPASy TrEMBL
Match: A0A1S3CRD1 (putative clathrin assembly protein At2g25430 OS=Cucumis melo OX=3656 GN=LOC103503830 PE=4 SV=1)

HSP 1 Score: 147.1 bits (370), Expect = 9.8e-32
Identity = 107/266 (40.23%), Postives = 125/266 (46.99%), Query Frame = 0


Query: 59  ALCPLVKESFQLYSDICEVLALLHDTI--RTVSRHLMRMILQGS---RLMSSLCFINWCK 118
           AL PLV+ESFQLY+DICEVLA+L D       S  +      GS   ++   + F NWCK
Sbjct: 276 ALYPLVRESFQLYADICEVLAVLLDKFFDMEYSDCMKAFDAYGSAAKQIDELIAFYNWCK 335

Query: 119 DVGVVRSSEYLEVRGIDCKVLETLD----------------------------------- 178
           D GV RSSEY EV+ I  K+LETL+                                   
Sbjct: 336 DTGVARSSEYPEVQRITSKLLETLEEFLRERGKRPKSPEREPPPPAPEEEEPAPDMNEIK 395

Query: 179 ---------------------------------------------TRLALALFAGLAANG 238
                                                         +LALALFAG AANG
Sbjct: 396 ALPPPENYTPPPPEPEPQPAPKPQPQVTDDLVNLRDDAVSADDQGNKLALALFAGPAANG 455

Query: 239 TNESWEAFPSSNRQPEVTHGRADACCWTWESGLGTGLDIDASNLTWERAALGGGFDPLLL 240
            N SWEAFPS   QPEVT           ++     L   ASNL+ ++AALGGG DPLLL
Sbjct: 456 ANGSWEAFPSDG-QPEVTSAWQTPAAEPGKADWELALVETASNLSRQKAALGGGLDPLLL 515

[Close]

BLAST of CmaCh09G004560 vs. ExPASy TrEMBL
Match: A0A6J1FFH4 (probable clathrin assembly protein At4g32285 OS=Cucurbita moschata OX=3662 GN=LOC111443478 PE=4 SV=1)

HSP 1 Score: 146.7 bits (369), Expect = 1.3e-31
Identity = 106/266 (39.85%), Postives = 125/266 (46.99%), Query Frame = 0


Query: 59  ALCPLVKESFQLYSDICEVLALLHDTI--RTVSRHLMRMILQGS---RLMSSLCFINWCK 118
           AL PLV+ESFQLY+DICEVLA+L D       S  +      GS   ++   + F NWCK
Sbjct: 273 ALYPLVRESFQLYADICEVLAVLLDKFFDMEYSDSMKAFDAYGSAAKQIDELIAFYNWCK 332

Query: 119 DVGVVRSSEYLEVRGIDCKVLETLD----------------------------------- 178
           D GV RSSEY EV+ I  K+LETL+                                   
Sbjct: 333 DTGVARSSEYPEVQRITSKLLETLEEFLRERGKAPKSPEREPPPPAPQEEEPVPDMNEIK 392

Query: 179 ---------------------------------------------TRLALALFAGLAANG 238
                                                         +LALALFAG AANG
Sbjct: 393 ALPPPENYTPPPPEPEPQPAPIPQPQVTEDLVNLRDDAVSADAQGNKLALALFAGPAANG 452

Query: 239 TNESWEAFPSSNRQPEVTHGRADACCWTWESGLGTGLDIDASNLTWERAALGGGFDPLLL 240
            N SWEAFPS   QPEVT           ++     L   ASNL+ ++AALGGG DPLLL
Sbjct: 453 ANGSWEAFPSDG-QPEVTSAWQTPAAEPGKADWELALVETASNLSRQKAALGGGLDPLLL 512

[Close]

BLAST of CmaCh09G004560 vs. ExPASy TrEMBL
Match: A0A6J1F990 (probable clathrin assembly protein At4g32285 OS=Cucurbita moschata OX=3662 GN=LOC111443479 PE=4 SV=1)

HSP 1 Score: 146.7 bits (369), Expect = 1.3e-31
Identity = 106/266 (39.85%), Postives = 125/266 (46.99%), Query Frame = 0


Query: 59  ALCPLVKESFQLYSDICEVLALLHDTI--RTVSRHLMRMILQGS---RLMSSLCFINWCK 118
           AL PLV+ESFQLY+DICEVLA+L D       S  +      GS   ++   + F NWCK
Sbjct: 273 ALYPLVRESFQLYADICEVLAVLLDKFFDMEYSDSMKAFDAYGSAAKQIDELIAFYNWCK 332

Query: 119 DVGVVRSSEYLEVRGIDCKVLETLD----------------------------------- 178
           D GV RSSEY EV+ I  K+LETL+                                   
Sbjct: 333 DTGVARSSEYPEVQRITSKLLETLEEFLRERGKAPKSPEREPPPPAPQEEEPVPDMNEIK 392

Query: 179 ---------------------------------------------TRLALALFAGLAANG 238
                                                         +LALALFAG AANG
Sbjct: 393 ALPPPENYTPPPPEPEPQPAPIPQPQVTEDLVNLRDDAVSADAQGNKLALALFAGPAANG 452

Query: 239 TNESWEAFPSSNRQPEVTHGRADACCWTWESGLGTGLDIDASNLTWERAALGGGFDPLLL 240
            N SWEAFPS   QPEVT           ++     L   ASNL+ ++AALGGG DPLLL
Sbjct: 453 ANGSWEAFPSDG-QPEVTSAWQTPAAEPGKADWELALVETASNLSRQKAALGGGLDPLLL 512

[Close]

BLAST of CmaCh09G004560 vs. NCBI nr
Match: KAG6591569.1 (putative clathrin assembly protein, partial [Cucurbita argyrosperma subsp. sororia] >KAG6591606.1 putative clathrin assembly protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 238.0 bits (606), Expect = 8.7e-59
Identity = 132/190 (69.47%), Postives = 140/190 (73.68%), Query Frame = 0


Query: 1   MYGGSTLPFRRLFRFIIFHSLNHCFQLAFKLQRLKNIVFAYSRPLTTDRILIVQDDFDAL 60
           MYGGSTLPFRRLFRFIIFHSLNHCFQLAFKLQRLKN+VFAYSRPL TDRILIVQ      
Sbjct: 1   MYGGSTLPFRRLFRFIIFHSLNHCFQLAFKLQRLKNVVFAYSRPL-TDRILIVQ------ 60

Query: 61  CPLVKESFQLYSDICEVLALLHDTIRTVS-----RHLMRMILQGSRLMSSLCFINWCKDV 120
                ESFQLYSDI EVLALLHD    +      +      + G  +     F NWCKDV
Sbjct: 61  -----ESFQLYSDIYEVLALLHDKFFGMEHPDCVKAFDAYDIAGKPIDELSVFYNWCKDV 120

Query: 121 GVVRSSEYLEVRGIDCKVLETLD------TRLALALFAGLAANGTNESWEAFPSSNRQPE 180
           GVVRSSEYLEVR IDCK  + +       +RLALALFAGLAANGTNESWEAFPSSNRQPE
Sbjct: 121 GVVRSSEYLEVRRIDCKKTDAVSSDDQGHSRLALALFAGLAANGTNESWEAFPSSNRQPE 178

[Close]

BLAST of CmaCh09G004560 vs. NCBI nr
Match: XP_022976220.1 (uncharacterized protein LOC111476675 [Cucurbita maxima])

HSP 1 Score: 174.5 bits (441), Expect = 1.2e-39
Identity = 95/139 (68.35%), Postives = 95/139 (68.35%), Query Frame = 0


Query: 1   MYGGSTLPFRRLFRFIIFHSLNHCFQLAFKLQRLKNIVFAYSRPLTTDRILIVQDDFDAL 60
           MYGGSTLPFRRLFRFIIFHSLNHCFQLAFKLQRLKNIVFAYSRPLTTDRILIV       
Sbjct: 1   MYGGSTLPFRRLFRFIIFHSLNHCFQLAFKLQRLKNIVFAYSRPLTTDRILIV------- 60

Query: 61  CPLVKESFQLYSDICEVLALLHDTIRTVSRHLMRMILQGSRLMSSLCFINWCKDVGVVRS 120
                                                QGSRLMSSLCFINWCKDVGVVRS
Sbjct: 61  -------------------------------------QGSRLMSSLCFINWCKDVGVVRS 95

Query: 121 SEYLEVRGIDCKVLETLDT 140
           SEYLEVRGIDCKVLETLDT
Sbjct: 121 SEYLEVRGIDCKVLETLDT 95

[Close]

BLAST of CmaCh09G004560 vs. NCBI nr
Match: XP_022976122.1 (putative clathrin assembly protein At2g25430 [Cucurbita maxima])

HSP 1 Score: 147.9 bits (372), Expect = 1.2e-31
Identity = 107/266 (40.23%), Postives = 125/266 (46.99%), Query Frame = 0


Query: 59  ALCPLVKESFQLYSDICEVLALLHDTI--RTVSRHLMRMILQGS---RLMSSLCFINWCK 118
           AL PLV+ESFQLY+DICEVLA+L D       S  +      GS   ++   + F NWCK
Sbjct: 275 ALYPLVRESFQLYADICEVLAVLLDKFFDMEYSDSMKAFDAYGSAAKQIDELIAFYNWCK 334

Query: 119 DVGVVRSSEYLEVRGIDCKVLETLD----------------------------------- 178
           D GV RSSEY EV+ I  K+LETL+                                   
Sbjct: 335 DTGVARSSEYPEVQRITSKLLETLEEFLRERGKAPKSPEREPPPPAPQEEEPVPDMNEIK 394

Query: 179 ---------------------------------------------TRLALALFAGLAANG 238
                                                         +LALALFAG AANG
Sbjct: 395 ALPPPENYTPPPPEPEPQPAPKPQPQVTEDLVNLRDDAVSADAQGNKLALALFAGPAANG 454

Query: 239 TNESWEAFPSSNRQPEVTHGRADACCWTWESGLGTGLDIDASNLTWERAALGGGFDPLLL 240
            N SWEAFPS   QPEVT           ++     L   ASNL+ ++AALGGG DPLLL
Sbjct: 455 ANVSWEAFPSDG-QPEVTSAWQTPAAEPGKADWELALVETASNLSRQKAALGGGLDPLLL 514

[Close]

BLAST of CmaCh09G004560 vs. NCBI nr
Match: XP_023535978.1 (probable clathrin assembly protein At4g32285 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 147.1 bits (370), Expect = 2.0e-31
Identity = 121/324 (37.35%), Postives = 146/324 (45.06%), Query Frame = 0


Query: 4   GSTLPFRRLFRFIIFHSLNHCFQLAFKLQRLKNIVFAYSRP---LTTDRILIVQDDFDAL 63
           G   P R +    IF  + H       LQRL +  F   RP     + R+++      AL
Sbjct: 228 GPVTPLREMSIERIFGKMGH-------LQRLLD-RFLSCRPTGLAKSSRMILY-----AL 287

Query: 64  CPLVKESFQLYSDICEVLALLHDTI--RTVSRHLMRMILQGS---RLMSSLCFINWCKDV 123
            PLV+ESFQLY+DICEVLA+L D       S  +      GS   ++   + F NWCKD 
Sbjct: 288 YPLVRESFQLYADICEVLAVLLDKFFDMEYSDCMKAFDAYGSAAKQIDELIAFYNWCKDT 347

Query: 124 GVVRSSEYLEVRGIDCKVLETLD------------------------------------- 183
           GV RSSEY EV+ I  K+LETL+                                     
Sbjct: 348 GVARSSEYPEVQRITSKLLETLEEFLRERGKAPKSPEREPPPPAPQEEEPVPDMNEIKAL 407

Query: 184 -------------------------------------------TRLALALFAGLAANGTN 240
                                                       +LALALFAG AANG N
Sbjct: 408 PPPENYTPPPPEPEPQPAPIPQPQVTEDLVNLRDDAVSADAQGNKLALALFAGPAANGAN 467

[Close]

BLAST of CmaCh09G004560 vs. NCBI nr
Match: XP_023535976.1 (probable clathrin assembly protein At4g32285 isoform X1 [Cucurbita pepo subsp. pepo] >XP_023535977.1 probable clathrin assembly protein At4g32285 isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 147.1 bits (370), Expect = 2.0e-31
Identity = 121/324 (37.35%), Postives = 146/324 (45.06%), Query Frame = 0


Query: 4   GSTLPFRRLFRFIIFHSLNHCFQLAFKLQRLKNIVFAYSRP---LTTDRILIVQDDFDAL 63
           G   P R +    IF  + H       LQRL +  F   RP     + R+++      AL
Sbjct: 228 GPVTPLREMSIERIFGKMGH-------LQRLLD-RFLSCRPTGLAKSSRMILY-----AL 287

Query: 64  CPLVKESFQLYSDICEVLALLHDTI--RTVSRHLMRMILQGS---RLMSSLCFINWCKDV 123
            PLV+ESFQLY+DICEVLA+L D       S  +      GS   ++   + F NWCKD 
Sbjct: 288 YPLVRESFQLYADICEVLAVLLDKFFDMEYSDCMKAFDAYGSAAKQIDELIAFYNWCKDT 347

Query: 124 GVVRSSEYLEVRGIDCKVLETLD------------------------------------- 183
           GV RSSEY EV+ I  K+LETL+                                     
Sbjct: 348 GVARSSEYPEVQRITSKLLETLEEFLRERGKAPKSPEREPPPPAPQEEEPVPDMNEIKAL 407

Query: 184 -------------------------------------------TRLALALFAGLAANGTN 240
                                                       +LALALFAG AANG N
Sbjct: 408 PPPENYTPPPPEPEPQPAPIPQPQVTEDLVNLRDDAVSADAQGNKLALALFAGPAANGAN 467

[Close]

BLAST of CmaCh09G004560 vs. TAIR 10
Match: AT4G32285.1 (ENTH/ANTH/VHS superfamily protein )

HSP 1 Score: 118.2 bits (295), Expect = 9.4e-27
Identity = 106/330 (32.12%), Postives = 138/330 (41.82%), Query Frame = 0


Query: 5   STLPFRRLFRFIIFHSLNHCFQLAFKLQRLKNIVFAYSRP---LTTDRILIVQDDFDALC 64
           S  P R +    IF  + H       LQRL +  F   RP       R++++     A+ 
Sbjct: 222 SVTPLREMTPERIFGKMGH-------LQRLLD-RFLSCRPTGLAKNSRMILI-----AMY 281

Query: 65  PLVKESFQLYSDICEVLALLHDTIRTVS-----RHLMRMILQGSRLMSSLCFINWCKDVG 124
           P+VKESF+LY+DICEVLA+L D    +      +          ++   + F +WCKD G
Sbjct: 282 PVVKESFRLYADICEVLAVLLDKFFDMEYTDCVKAFDAYASAAKQIDELIAFYHWCKDTG 341

Query: 125 VVRSSEYLEVRGIDCKVLETLD-------------------------------------- 184
           V RSSEY EV+ I  K+LETL+                                      
Sbjct: 342 VARSSEYPEVQRITSKLLETLEEFVRDRAKRAKSPERKEIEAPPAPAPPVEEPVDMNEIK 401

Query: 185 -------------------------------------------TRLALALFAGLAANGTN 240
                                                       + ALALFAG  AN  N
Sbjct: 402 ALPPPENHTPPPPPAPEPKPQQPQVTDDLVNLREDDVSGDDQGNKFALALFAGPPAN--N 461

[Close]

BLAST of CmaCh09G004560 vs. TAIR 10
Match: AT4G32285.2 (ENTH/ANTH/VHS superfamily protein )

HSP 1 Score: 118.2 bits (295), Expect = 9.4e-27
Identity = 106/330 (32.12%), Postives = 138/330 (41.82%), Query Frame = 0


Query: 5   STLPFRRLFRFIIFHSLNHCFQLAFKLQRLKNIVFAYSRP---LTTDRILIVQDDFDALC 64
           S  P R +    IF  + H       LQRL +  F   RP       R++++     A+ 
Sbjct: 222 SVTPLREMTPERIFGKMGH-------LQRLLD-RFLSCRPTGLAKNSRMILI-----AMY 281

Query: 65  PLVKESFQLYSDICEVLALLHDTIRTVS-----RHLMRMILQGSRLMSSLCFINWCKDVG 124
           P+VKESF+LY+DICEVLA+L D    +      +          ++   + F +WCKD G
Sbjct: 282 PVVKESFRLYADICEVLAVLLDKFFDMEYTDCVKAFDAYASAAKQIDELIAFYHWCKDTG 341

Query: 125 VVRSSEYLEVRGIDCKVLETLD-------------------------------------- 184
           V RSSEY EV+ I  K+LETL+                                      
Sbjct: 342 VARSSEYPEVQRITSKLLETLEEFVRDRAKRAKSPERKEIEAPPAPAPPVEEPVDMNEIK 401

Query: 185 -------------------------------------------TRLALALFAGLAANGTN 240
                                                       + ALALFAG  AN  N
Sbjct: 402 ALPPPENHTPPPPPAPEPKPQQPQVTDDLVNLREDDVSGDDQGNKFALALFAGPPAN--N 461

[Close]

BLAST of CmaCh09G004560 vs. TAIR 10
Match: AT2G25430.1 (epsin N-terminal homology (ENTH) domain-containing protein / clathrin assembly protein-related )

HSP 1 Score: 113.6 bits (283), Expect = 2.3e-25
Identity = 101/323 (31.27%), Postives = 133/323 (41.18%), Query Frame = 0


Query: 8   PFRRLFRFIIFHSLNHCFQLAFKLQRLKNIVFAYSRPLTTDRILIVQDDFDALCPLVKES 67
           P R +    IF  + H  +L  +   L+    A        R++++     AL P+V+ES
Sbjct: 245 PLREMTPERIFGKMGHLQRLLDRFLSLRPTGLA-----KNSRMILI-----ALYPVVRES 304

Query: 68  FQLYSDICEVLALLHDTIRTVS-----RHLMRMILQGSRLMSSLCFINWCKDVGVVRSSE 127
           F+LY+DICEVLA+L D    +      +          ++   + F NWCK+ GV RSSE
Sbjct: 305 FKLYADICEVLAVLLDKFFDMEYSDCVKAFDAYASAAKQIDELIAFYNWCKETGVARSSE 364

Query: 128 YLEVRGIDCKVLETLD-------------------------------------------- 187
           Y EV+ I  K+LETL+                                            
Sbjct: 365 YPEVQRITSKLLETLEEFVRDRAKRGKSPERKEIEAPPPVVEEEEPEPDMNEIKALPPPE 424

Query: 188 -------------------------------------TRLALALFAGLAANGTNESWEAF 240
                                                 + ALALFAG    G N  WEAF
Sbjct: 425 NYTPPPPPEPEPQPEKPQFTEDLVNLREDEVTADDQGNKFALALFAG--PPGNNGKWEAF 484

[Close]

BLAST of CmaCh09G004560 vs. TAIR 10
Match: AT1G03050.1 (ENTH/ANTH/VHS superfamily protein )

HSP 1 Score: 70.5 bits (171), Expect = 2.2e-12
Identity = 85/325 (26.15%), Postives = 124/325 (38.15%), Query Frame = 0


Query: 26  QLAFKLQRLKNIV--FAYSRP---LTTDRILIVQDDFDALCPLVKESFQLYSDICEVLAL 85
           Q+  ++Q L+ ++  F   RP      +R++IV     AL P+VKESFQ+Y D+ E++ +
Sbjct: 202 QIFIRIQHLQQLLDRFLACRPTGNARNNRVVIV-----ALYPIVKESFQIYYDVTEIMGI 261

Query: 86  LHDTIRTV----SRHLMRMILQGSRLMSSL-CFINWCKDVGVVRSSEYLEVRGIDCKVLE 145
           L +    +    S  +  +  + S+    L  F +WCK++G+ RSSEY E+  I  K L+
Sbjct: 262 LIERFMELDIPDSIKVYDIFCRVSKQFEELDQFYSWCKNMGIARSSEYPEIEKITQKKLD 321

Query: 146 TLD--------------------------------------------------------- 205
            +D                                                         
Sbjct: 322 LMDEFIRDKSALEHTKQSKSVKSEADEDDDEARTEEVNEEQEDMNAIKALPEPPPKEEDD 381

Query: 206 --------------------------------------TRLALALFAGLAANGTNE---- 240
                                                   LALALF G  A+G+      
Sbjct: 382 VKPEEEAKEEVIIEKKQEEMGDLLDLGNTNGGEAGQAGDSLALALFDGPYASGSGSESGP 441

[Close]

BLAST of CmaCh09G004560 vs. TAIR 10
Match: AT4G02650.1 (ENTH/ANTH/VHS superfamily protein )

HSP 1 Score: 60.8 bits (146), Expect = 1.8e-09
Identity = 81/320 (25.31%), Postives = 119/320 (37.19%), Query Frame = 0


Query: 30  KLQRLKNIV--FAYSRP---LTTDRILIVQDDFDALCPLVKESFQLYSDICEVLALLHDT 89
           ++Q L+ ++  F   RP      +R++IV     A+ P+VKESFQLY +I E++ +L + 
Sbjct: 211 RVQHLQQLLDRFLACRPTGNAKNNRVVIV-----AMYPIVKESFQLYYNITEIMGVLIER 270

Query: 90  IRTVSRH----LMRMILQGSRLMSSL-CFINWCKDVGVVRSSEYLEVRGIDCKVLETLD- 149
              +  H    +  +  + S+    L  F  WCK++ V RSSEY E+  I  K L+ +D 
Sbjct: 271 FMELDIHDSIKVYEIFCRVSKQFDELDPFYGWCKNMAVARSSEYPELEKITQKKLDLMDE 330

Query: 150 ------------------------------------------------------------ 209
                                                                       
Sbjct: 331 FIRDKSALAAQTTKSSSKRSNKSEEEESKTEYIQENQEDLNSIKALPAPEQKEEEEEEEK 390

Query: 210 -----------------------------------TRLALALFAGLAA--NGTNESWEAF 240
                                                LALALF G+    + +   WEAF
Sbjct: 391 METKKDVEEVVSRQDQEGDLLDLTDEAGVTAGTVGDSLALALFDGVVGTESASGPGWEAF 450

The following BLAST results are available for this feature:

BLAST of CmaCh09G004560 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8S9J8	1.3e-25	32.12	Probable clathrin assembly protein At4g32285 OS=Arabidopsis thaliana OX=3702 GN=...	[more]
Q8LF20	3.3e-24	31.27	Putative clathrin assembly protein At2g25430 OS=Arabidopsis thaliana OX=3702 GN=...	[more]
Q9SA65	3.2e-11	26.15	Putative clathrin assembly protein At1g03050 OS=Arabidopsis thaliana OX=3702 GN=...	[more]
Q8GX47	2.5e-08	25.31	Putative clathrin assembly protein At4g02650 OS=Arabidopsis thaliana OX=3702 GN=...	[more]

BLAST of CmaCh09G004560 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1IIW0	5.7e-40	68.35	uncharacterized protein LOC111476675 OS=Cucurbita maxima OX=3661 GN=LOC111476675...	[more]
A0A6J1IEX9	5.8e-32	40.23	putative clathrin assembly protein At2g25430 OS=Cucurbita maxima OX=3661 GN=LOC1...	[more]
A0A1S3CRD1	9.8e-32	40.23	putative clathrin assembly protein At2g25430 OS=Cucumis melo OX=3656 GN=LOC10350...	[more]
A0A6J1FFH4	1.3e-31	39.85	probable clathrin assembly protein At4g32285 OS=Cucurbita moschata OX=3662 GN=LO...	[more]
A0A6J1F990	1.3e-31	39.85	probable clathrin assembly protein At4g32285 OS=Cucurbita moschata OX=3662 GN=LO...	[more]

BLAST of CmaCh09G004560 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG6591569.1	8.7e-59	69.47	putative clathrin assembly protein, partial [Cucurbita argyrosperma subsp. soror...	[more]
XP_022976220.1	1.2e-39	68.35	uncharacterized protein LOC111476675 [Cucurbita maxima]	[more]
XP_022976122.1	1.2e-31	40.23	putative clathrin assembly protein At2g25430 [Cucurbita maxima]	[more]
XP_023535978.1	2.0e-31	37.35	probable clathrin assembly protein At4g32285 [Cucurbita pepo subsp. pepo]	[more]
XP_023535976.1	2.0e-31	37.35	probable clathrin assembly protein At4g32285 isoform X1 [Cucurbita pepo subsp. p...	[more]

BLAST of CmaCh09G004560 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G32285.1	9.4e-27	32.12	ENTH/ANTH/VHS superfamily protein	[more]
AT4G32285.2	9.4e-27	32.12	ENTH/ANTH/VHS superfamily protein	[more]
AT2G25430.1	2.3e-25	31.27	epsin N-terminal homology (ENTH) domain-containing protein / clathrin assembly p...	[more]
AT1G03050.1	2.2e-12	26.15	ENTH/ANTH/VHS superfamily protein	[more]
AT4G02650.1	1.8e-09	25.31	ENTH/ANTH/VHS superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR011417	AP180 N-terminal homology (ANTH) domain	PFAM	PF07651	ANTH	coord: 59..139 e-value: 3.4E-8 score: 32.8
None	No IPR available	PANTHER	PTHR22951:SF91	ENTH/ANTH/VHS SUPERFAMILY PROTEIN-RELATED	coord: 140..239 coord: 30..138
None	No IPR available	PANTHER	PTHR22951	CLATHRIN ASSEMBLY PROTEIN	coord: 140..239 coord: 30..138
None	No IPR available	SUPERFAMILY	89009	GAT-like domain	coord: 59..138

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmaCh09G004560.1	CmaCh09G004560.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0005543	phospholipid binding