CmaCh09G002670 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh09G002670
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
DescriptionCytochrome P450
LocationCma_Chr09: 1109388 .. 1111813 (-)
RNA-Seq ExpressionCmaCh09G002670
SyntenyCmaCh09G002670
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATCATTTTTTCTTCACCTCTTTCTCAAATGTCTTCTAATATATCCGTTCCCTAACTCTAGCTTATCCCAATTGCCCACCCTGCTAATTTCTTGTATAAAACCATGCAAAATTGAGCCCATTTTGGATATGCACCTGCAACAACTTCCAACATATTCATCCATGGAGGCTAACACGATTACATGCCTCTCTCTCTCGCTTCTCTTGACTCTTATTCTTATTTACAAACTCAGAAAACAAAGCCCCAGAAACCGTCCTCCCAGCCCACCATCGCTGCCTCTCATCGGCCACCTTCATCTCCTCAAACAACCTTTCCACAGAGTGCTGCAGGATCTCTCCAACAAACACGGTCCAATTTTATCCCTTTCAATCGGTTCCCGTCCGCTGGTGGTTATTTCCTCCCCAACCGCTGTCCGCGAGTGCTTTACCAAAAACGACATCGCCTTGGCCAACCGCCCTCGCTTGCTTGCTGGGAAGTACATGAACTACAACTACACCGGTGTGGCTTTTGCCTCCTACGGCCACCACTGGCGCAACATGCGCCGCATAGCCACCACAGAGCTACTCTCCAACCACCGCCTCAACGCGTATCTCAATATCCGAGTGCAAGAGCTGAAATTGGTCAAGAATTTGTACGGCGCGACAGGGGAAAGTAGTGATTTTGTTCGCTTGGAGATGCAGTCCAAATTGAACGAGCTTTCTTTTAATATTGTGATGAGGATGGTGAGTGGGAAACGTTACTTTGGTGTAGATGTTGAGGATGTTGAGGAAGCTAGGGAGTTTAGGGAGATCATGAAGGAGCTTCTTCAATTGGGCGGGGCGTCGAATCCAGCCGATTTTTTGCCCATTTTGAGACTCTTTGATTATCAAGGACTTGAGAAGAGGATGGTGCAAGCGAGTGGTCGAGCTGATTCGTTCCTACAAAATCTGATTGATTGCGAGCGGGATAGGCGAGCTTCTCGTTGGCCGGAGGAAAAACGTGGAAACAAGACCATGATTCAGAGCCTGCTGTCCTTGCAAGAATCTCAACCTCAACATTACTCCGATGATATCATCAAGGGTCATGTTCTGGTAACTTCAATCCCTCCCAATTCCCTCCCATCATCCCATTTTAATTGTGTGAGATCATACATTAAACAATTAAAGAATAAGGGTGTGAAAACTTATTCCTATCTTGAAAGGAAAGTTCGTAAGGAAAAACTAAAAAAAGGCACTATTGTTGTTACAAATAGTATCAACGCTGGACACCGAATGAAGACGTGTTCAGTGAGAAGTTTTAGTCCTGAAAGGGATAGATATTAGGTGGTGTGCCTACGAGGGCGCAGGCTACGAAAGGGTGGATAGTGAGATCTCACATCGGTTGAGAAGGAGAACGAAACATTCTTTATAAGGGTGTGGAAACCTTTCGGGATGTACTGTGAGATCTCACATTGACTGGGTGGGGAGGAGAACCAAACATTATTTATAAAGATGTAATACTGTATATAGCCACACAATTTCAAAACTTTGAAAGAAAGGAAACTCACAACAAAAAAGTCAAAATACGATGCTATCAAAGGAAACTCACGAAAAATATGACACTATCTAGCGTTGGGCGCATGCCGATCAGGTTGGTGCTTCCAACCACCATTTGTGACGTGACAAACACTTCAATATTATTTGTTATTTTGACATCATAAATTGTGAAGTTTGGTGGATATCTACTGCAGACGATGTTGGCTGCGGGTACGGATACAACTACAGGAACATTAGAATGGGCGATGTCGCTGCTTCTGAATCATCCCGCGATAATGAATAAAGCTTGGAGCGAGATAAGAGAGTGCGTTGGGGAGAGTCGGCTGGTGGAGGAAGGCGATGTGAGCAGCCTAAAATACTTGGTAGCCATAATCTACGAGACTCTTCGACTGTTTCCAGCAGCTCCTCTGTTGGTGCCACACGAATCCTCTGAAGAGTGTAGAGTTGAAGGATTTGACATACCCAAAGGCACGATGCTATTGGTGAATGCATGGGCCATCCATAGGGACCCTCGAGTGTGGGAGGATCCCACTAGCTTCCGACCTGAGAGGTTTCTGAATTGGGAAACTGCTGAGTCCCACAAATGGGTGCCGTTTGGAGTGGGGAGGAGGGCTTGTCCTGGGGCTGCCCTCGCCCACCGTGTCGTGGGCTTGACGCTGGCCACTTTGATTCAGTGCTTTGAGTGGCAGAGAGTTGGCGAAGAGCCCATAGACTTGTCCCAGGGAACTGGAATCACTACGCCCAAAGCCATACCTTTGGAGGCAATGTGCAGAGCCCGTAGCTCCATGCTTCATTTACTCTCTCCGCTTTGATTTTACAAGAATAAACTTGTTAAATGTTATCAAGATTTCTGTAGTATTAAGCTGTAAAAGTAAGATAATATCTTCTTAATAAATGTTTCTTTAAAAATTAA

mRNA sequence

ATCATTTTTTCTTCACCTCTTTCTCAAATGTCTTCTAATATATCCGTTCCCTAACTCTAGCTTATCCCAATTGCCCACCCTGCTAATTTCTTGTATAAAACCATGCAAAATTGAGCCCATTTTGGATATGCACCTGCAACAACTTCCAACATATTCATCCATGGAGGCTAACACGATTACATGCCTCTCTCTCTCGCTTCTCTTGACTCTTATTCTTATTTACAAACTCAGAAAACAAAGCCCCAGAAACCGTCCTCCCAGCCCACCATCGCTGCCTCTCATCGGCCACCTTCATCTCCTCAAACAACCTTTCCACAGAGTGCTGCAGGATCTCTCCAACAAACACGGTCCAATTTTATCCCTTTCAATCGGTTCCCGTCCGCTGGTGGTTATTTCCTCCCCAACCGCTGTCCGCGAGTGCTTTACCAAAAACGACATCGCCTTGGCCAACCGCCCTCGCTTGCTTGCTGGGAAGTACATGAACTACAACTACACCGGTGTGGCTTTTGCCTCCTACGGCCACCACTGGCGCAACATGCGCCGCATAGCCACCACAGAGCTACTCTCCAACCACCGCCTCAACGCGTATCTCAATATCCGAGTGCAAGAGCTGAAATTGGTCAAGAATTTGTACGGCGCGACAGGGGAAAGTAGTGATTTTGTTCGCTTGGAGATGCAGTCCAAATTGAACGAGCTTTCTTTTAATATTGTGATGAGGATGGTGAGTGGGAAACGTTACTTTGGTGTAGATGTTGAGGATGTTGAGGAAGCTAGGGAGTTTAGGGAGATCATGAAGGAGCTTCTTCAATTGGGCGGGGCGTCGAATCCAGCCGATTTTTTGCCCATTTTGAGACTCTTTGATTATCAAGGACTTGAGAAGAGGATGGTGCAAGCGAGTGGTCGAGCTGATTCGTTCCTACAAAATCTGATTGATTGCGAGCGGGATAGGCGAGCTTCTCGTTGGCCGGAGGAAAAACGTGGAAACAAGACCATGATTCAGAGCCTGCTGTCCTTGCAAGAATCTCAACCTCAACATTACTCCGATGATATCATCAAGGGTCATGTTCTGACGATGTTGGCTGCGGGTACGGATACAACTACAGGAACATTAGAATGGGCGATGTCGCTGCTTCTGAATCATCCCGCGATAATGAATAAAGCTTGGAGCGAGATAAGAGAGTGCGTTGGGGAGAGTCGGCTGGTGGAGGAAGGCGATGTGAGCAGCCTAAAATACTTGGTAGCCATAATCTACGAGACTCTTCGACTGTTTCCAGCAGCTCCTCTGTTGGTGCCACACGAATCCTCTGAAGAGTGTAGAGTTGAAGGATTTGACATACCCAAAGGCACGATGCTATTGGTGAATGCATGGGCCATCCATAGGGACCCTCGAGTGTGGGAGGATCCCACTAGCTTCCGACCTGAGAGGTTTCTGAATTGGGAAACTGCTGAGTCCCACAAATGGGTGCCGTTTGGAGTGGGGAGGAGGGCTTGTCCTGGGGCTGCCCTCGCCCACCGTGTCGTGGGCTTGACGCTGGCCACTTTGATTCAGTGCTTTGAGTGGCAGAGAGTTGGCGAAGAGCCCATAGACTTGTCCCAGGGAACTGGAATCACTACGCCCAAAGCCATACCTTTGGAGGCAATGTGCAGAGCCCGTAGCTCCATGCTTCATTTACTCTCTCCGCTTTGATTTTACAAGAATAAACTTGTTAAATGTTATCAAGATTTCTGTAGTATTAAGCTGTAAAAGTAAGATAATATCTTCTTAATAAATGTTTCTTTAAAAATTAA

Coding sequence (CDS)

ATGCACCTGCAACAACTTCCAACATATTCATCCATGGAGGCTAACACGATTACATGCCTCTCTCTCTCGCTTCTCTTGACTCTTATTCTTATTTACAAACTCAGAAAACAAAGCCCCAGAAACCGTCCTCCCAGCCCACCATCGCTGCCTCTCATCGGCCACCTTCATCTCCTCAAACAACCTTTCCACAGAGTGCTGCAGGATCTCTCCAACAAACACGGTCCAATTTTATCCCTTTCAATCGGTTCCCGTCCGCTGGTGGTTATTTCCTCCCCAACCGCTGTCCGCGAGTGCTTTACCAAAAACGACATCGCCTTGGCCAACCGCCCTCGCTTGCTTGCTGGGAAGTACATGAACTACAACTACACCGGTGTGGCTTTTGCCTCCTACGGCCACCACTGGCGCAACATGCGCCGCATAGCCACCACAGAGCTACTCTCCAACCACCGCCTCAACGCGTATCTCAATATCCGAGTGCAAGAGCTGAAATTGGTCAAGAATTTGTACGGCGCGACAGGGGAAAGTAGTGATTTTGTTCGCTTGGAGATGCAGTCCAAATTGAACGAGCTTTCTTTTAATATTGTGATGAGGATGGTGAGTGGGAAACGTTACTTTGGTGTAGATGTTGAGGATGTTGAGGAAGCTAGGGAGTTTAGGGAGATCATGAAGGAGCTTCTTCAATTGGGCGGGGCGTCGAATCCAGCCGATTTTTTGCCCATTTTGAGACTCTTTGATTATCAAGGACTTGAGAAGAGGATGGTGCAAGCGAGTGGTCGAGCTGATTCGTTCCTACAAAATCTGATTGATTGCGAGCGGGATAGGCGAGCTTCTCGTTGGCCGGAGGAAAAACGTGGAAACAAGACCATGATTCAGAGCCTGCTGTCCTTGCAAGAATCTCAACCTCAACATTACTCCGATGATATCATCAAGGGTCATGTTCTGACGATGTTGGCTGCGGGTACGGATACAACTACAGGAACATTAGAATGGGCGATGTCGCTGCTTCTGAATCATCCCGCGATAATGAATAAAGCTTGGAGCGAGATAAGAGAGTGCGTTGGGGAGAGTCGGCTGGTGGAGGAAGGCGATGTGAGCAGCCTAAAATACTTGGTAGCCATAATCTACGAGACTCTTCGACTGTTTCCAGCAGCTCCTCTGTTGGTGCCACACGAATCCTCTGAAGAGTGTAGAGTTGAAGGATTTGACATACCCAAAGGCACGATGCTATTGGTGAATGCATGGGCCATCCATAGGGACCCTCGAGTGTGGGAGGATCCCACTAGCTTCCGACCTGAGAGGTTTCTGAATTGGGAAACTGCTGAGTCCCACAAATGGGTGCCGTTTGGAGTGGGGAGGAGGGCTTGTCCTGGGGCTGCCCTCGCCCACCGTGTCGTGGGCTTGACGCTGGCCACTTTGATTCAGTGCTTTGAGTGGCAGAGAGTTGGCGAAGAGCCCATAGACTTGTCCCAGGGAACTGGAATCACTACGCCCAAAGCCATACCTTTGGAGGCAATGTGCAGAGCCCGTAGCTCCATGCTTCATTTACTCTCTCCGCTTTGA

Protein sequence

MHLQQLPTYSSMEANTITCLSLSLLLTLILIYKLRKQSPRNRPPSPPSLPLIGHLHLLKQPFHRVLQDLSNKHGPILSLSIGSRPLVVISSPTAVRECFTKNDIALANRPRLLAGKYMNYNYTGVAFASYGHHWRNMRRIATTELLSNHRLNAYLNIRVQELKLVKNLYGATGESSDFVRLEMQSKLNELSFNIVMRMVSGKRYFGVDVEDVEEAREFREIMKELLQLGGASNPADFLPILRLFDYQGLEKRMVQASGRADSFLQNLIDCERDRRASRWPEEKRGNKTMIQSLLSLQESQPQHYSDDIIKGHVLTMLAAGTDTTTGTLEWAMSLLLNHPAIMNKAWSEIRECVGESRLVEEGDVSSLKYLVAIIYETLRLFPAAPLLVPHESSEECRVEGFDIPKGTMLLVNAWAIHRDPRVWEDPTSFRPERFLNWETAESHKWVPFGVGRRACPGAALAHRVVGLTLATLIQCFEWQRVGEEPIDLSQGTGITTPKAIPLEAMCRARSSMLHLLSPL
Homology
[Close]
BLAST of CmaCh09G002670 vs. ExPASy Swiss-Prot
Match: W8JMU7 (Cytochrome P450 81Q32 OS=Catharanthus roseus OX=4058 GN=CYP81Q32 PE=2 SV=1)

HSP 1 Score: 531.9 bits (1369), Expect = 7.8e-150
Identity = 271/510 (53.14%), Postives = 369/510 (72.35%), Query Frame = 0 

Query: 12  MEANTI--TCLSLSLLLTLILIYKLRKQSPRNRPPSPP-SLPLIGHLHLLKQPFHRVLQD 71
           ME++T+  T L++ LL   + ++ + ++  RN PPSP  +LP+IGHLHL+ +  HR L D
Sbjct: 1   MESSTLLYTFLAVVLLSISLKLFPVSRRR-RNLPPSPGLALPVIGHLHLIGKLLHRSLYD 60

Query: 72  LSNKHGPILSLSIGSRPLVVISSPTAVRECFTKNDIALANRPRLLAGKYMNYNYTGVAFA 131
           LS K+G + SL +G+R ++V+SSP A  ECFTKNDI  ANRP  + GKY+ YNYT +  +
Sbjct: 61  LSKKYGSVFSLQLGNRLVLVVSSPAAAEECFTKNDIVFANRPLFILGKYIGYNYTTMVGS 120

Query: 132 SYGHHWRNMRRIATTELLSNHRLNAYLNIRVQELK-LVKNLYGATGESSDFVRLEMQSKL 191
            YG HWRN+RR+A  E+ S   LN +L+IR  E+K L+ +LY ++G+  DF ++EM+SKL
Sbjct: 121 PYGEHWRNLRRLAAVEIFSAGSLNRFLSIREDEVKQLLLSLYQSSGQ--DFGKVEMKSKL 180

Query: 192 NELSFNIVMRMVSGKRYFGVDVEDVEEAREFREIMKELLQLGGASNPADFLPILRLFDYQ 251
           +ELSFN+ MRMV+GKRYFG DV D +EA+ FR ++ E+ +  GASNP DF+P LR  D++
Sbjct: 181 SELSFNVTMRMVAGKRYFGQDV-DSDEAKLFRALIGEVFEHAGASNPGDFVPFLRWIDFK 240

Query: 252 GLEKRMVQASGRADSFLQNLIDCERDRRASRWPEEKRGNKTMIQSLLSLQESQPQHYSDD 311
             EK++ + S   D+FLQ LI   R           + N TMI  LLSLQESQP++Y+D 
Sbjct: 241 NYEKKVSKISQEMDAFLQRLIHESR---------INKNNVTMIDHLLSLQESQPEYYTDQ 300

Query: 312 IIKGHVLTMLAAGTDTTTGTLEWAMSLLLNHPAIMNKAWSEIRECVGESRLVEEGDVSSL 371
           IIKG ++ +L AGTDT+  T+EWAMSLLLNHP  + KA +EI   VG +RL+EE D+  L
Sbjct: 301 IIKGIIMVLLLAGTDTSAVTVEWAMSLLLNHPETLEKARTEIETQVGSNRLIEEQDLPKL 360

Query: 372 KYLVAIIYETLRLFPAAPLLVPHESSEECRVEGFDIPKGTMLLVNAWAIHRDPRVWEDPT 431
            YL  II ET RL PAAP+LVPHESS++C+V+G+D+PKGT+LLVNAWAIHRDP  W++PT
Sbjct: 361 TYLHNIISETFRLCPAAPMLVPHESSDDCKVQGYDVPKGTILLVNAWAIHRDPEFWDEPT 420

Query: 432 SFRPERFLNWETAESHKWVPFGVGRRACPGAALAHRVVGLTLATLIQCFEWQRVGEEPID 491
            F+PER    E  E  K +PFG+GRR+CPG+ LA RVVGLTL  LIQCFEW+R+GE  ID
Sbjct: 421 LFKPERHGGVE-LEPSKLMPFGMGRRSCPGSGLAQRVVGLTLGALIQCFEWKRIGEAKID 480

Query: 492 LSQGTGITTPKAIPLEAMCRARSSMLHLLS 518
           +++G+G+T PKA PLEA+C+ R+ +  ++S
Sbjct: 481 MAEGSGLTMPKAQPLEALCKPRNILHKVVS 496

[Close]
BLAST of CmaCh09G002670 vs. ExPASy Swiss-Prot
Match: Q6WNQ9 (Isoflavone 3'-hydroxylase (Fragment) OS=Medicago truncatula OX=3880 GN=CYP81E9 PE=1 SV=1)

HSP 1 Score: 510.0 bits (1312), Expect = 3.2e-143
Identity = 256/498 (51.41%), Postives = 342/498 (68.67%), Query Frame = 0 

Query: 20  LSLSLLLTLILIYKLRKQSPRNRPPSPPSLPLIGHLHLLKQPFHRVLQDLSNKHGPILSL 79
           LSLS ++T+ ++ K+  +  +N PP PP++P+IG+LH LK P HR    LS  +G I SL
Sbjct: 8   LSLSFIITIKILLKITSRRLKNLPPGPPTIPIIGNLHHLKHPLHRTFTTLSQTYGDIFSL 67

Query: 80  SIGSRPLVVISSPTAVRECFTKNDIALANRPRLLAGKYMNYNYTGVAFASYGHHWRNMRR 139
             GSR +VV+SSP+   ECFTKNDI LANRPR L GKY+ YNYT +  ASYG HWRN+RR
Sbjct: 68  WFGSRLVVVVSSPSLAHECFTKNDIILANRPRFLTGKYIFYNYTTLGSASYGDHWRNLRR 127

Query: 140 IATTELLSNHRLNAYLNIRVQELK--LVKNLYGATGESSDFVRLEMQSKLNELSFNIVMR 199
           I T ++LSN+RLN++L +R  E    + K L     E   F ++E++ +L E++FN +MR
Sbjct: 128 ITTIDVLSNNRLNSFLGVRRDETNRLIQKLLKDVVSEGFGFTKVELRPRLTEMTFNAMMR 187

Query: 200 MVSGKRYFG--VDVEDVEEAREFREIMKELLQLGGASNPADFLPILRLFDYQGLEKRMVQ 259
           M+SGKRY+G   DV DVEEA++FREI+ E++ L GA+N  DFLP+LR+ D   LEKR  +
Sbjct: 188 MISGKRYYGDDGDVSDVEEAKQFREIISEMMSLLGANNKGDFLPLLRVVDLDNLEKRCKR 247

Query: 260 ASGRADSFLQNLIDCERDRRASRWPEEKRGN-----KTMIQSLLSLQESQPQHYSDDIIK 319
            + R+++FL+ LI+           E +RGN      TMI  LL L ESQP++YSD +IK
Sbjct: 248 IAKRSNAFLEGLIE-----------EHRRGNIHSDGGTMIDHLLKLSESQPEYYSDHLIK 307

Query: 320 GHVLTMLAAGTDTTTGTLEWAMSLLLNHPAIMNKAWSEIRECVGESRLVEEGDVSSLKYL 379
           G +  ML AGTDT+  T+EW MS LLNHP ++ KA  E+   +G+++LV+E D+S L YL
Sbjct: 308 GLIQGMLLAGTDTSAVTIEWVMSELLNHPEVLKKAKEELDTQIGKNKLVDEQDLSKLPYL 367

Query: 380 VAIIYETLRLFPAAPLLVPHESSEECRVEGFDIPKGTMLLVNAWAIHRDPRVWEDPTSFR 439
             II ETLRL P APLL+PH SSE+C +  F++PK T++L N W IHRDP+ W D  SF+
Sbjct: 368 QNIISETLRLHPPAPLLLPHYSSEDCTIGEFNVPKDTIILTNVWGIHRDPKHWNDALSFK 427

Query: 440 PERFLNWETAESHKWVPFGVGRRACPGAALAHRVVGLTLATLIQCFEWQRVGEEPIDLSQ 499
           PERF   E  E +K + FG+GRRACPG +LA R VG T+  LIQCFEW+R  EE +D+ +
Sbjct: 428 PERFEKEE--EVNKVMAFGLGRRACPGLSLAQRTVGFTVGLLIQCFEWERESEEKLDMME 487

Query: 500 GTGITTPKAIPLEAMCRA 509
           G GIT P  IPL AMC+A
Sbjct: 488 GKGITMPMKIPLRAMCKA 492

[Close]
BLAST of CmaCh09G002670 vs. ExPASy Swiss-Prot
Match: Q6WNQ8 (Cytochrome P450 81E8 OS=Medicago truncatula OX=3880 GN=CYP81E8 PE=2 SV=1)

HSP 1 Score: 508.1 bits (1307), Expect = 1.2e-142
Identity = 262/493 (53.14%), Postives = 341/493 (69.17%), Query Frame = 0 

Query: 20  LSLSLLLTLILIYKLRKQSPRNRPPSPPSLPLIGHLHLLKQPFHRVLQDLSNKHGPILSL 79
           +SL  L+  + ++    +  +N PP P  LP+IG+LH LKQP H     LS K+G I SL
Sbjct: 10  ISLFFLIITLKVFFNTSRKFKNLPPGPQCLPIIGNLHQLKQPLHHTFHTLSQKYGQIFSL 69

Query: 80  SIGSRPLVVISSPTAVRECFTKNDIALANRPRLLAGKYMNYNYTGVAFASYGHHWRNMRR 139
             GSR +VV+SS T  +ECFTKNDI LANRP  L GKY+ YN T VA + YG HWRN+RR
Sbjct: 70  WFGSRLVVVVSSLTIAQECFTKNDIVLANRPHFLTGKYIGYNNTTVAQSPYGDHWRNLRR 129

Query: 140 IATTELLSNHRLNAYLNIRVQE-LKLVKNLYGATGESSDFVRLEMQSKLNELSFNIVMRM 199
           I + E+LS+HRLN++L IR  E ++L++ L  A    + F  +E++   +E++FN +MRM
Sbjct: 130 ILSIEILSSHRLNSFLEIRRDEIMRLIQKL--AQKSYNGFTEVELRPMFSEMTFNTIMRM 189

Query: 200 VSGKRYFG--VDVEDVEEAREFREIMKELLQLGGASNPADFLPILRLFDYQGLEKRMVQA 259
           VSGKRY+G   DV DVEEAR FR I+KE++ LGGA+N  DFL  LR FD+ GLEKR+ + 
Sbjct: 190 VSGKRYYGNDCDVSDVEEARLFRGIIKEVVSLGGANNVGDFLGFLRWFDFDGLEKRLKKI 249

Query: 260 SGRADSFLQNLIDCERDRRASRWPEEKRGNKTMIQSLLSLQESQPQHYSDDIIKGHVLTM 319
           S R D+FLQ LID  R          KR + TMI  LL+ Q+SQP++Y+D IIKG ++ M
Sbjct: 250 SKRTDAFLQGLIDEHR--------FGKRNSNTMIDHLLTQQQSQPEYYTDQIIKGLMVVM 309

Query: 320 LAAGTDTTTGTLEWAMSLLLNHPAIMNKAWSEIRECVGESRLVEEGDVSSLKYLVAIIYE 379
           L AGTDT++ T+EWAMS LLNHP IM KA +E+   +G  R V+E D+S L YL +I+YE
Sbjct: 310 LLAGTDTSSVTIEWAMSNLLNHPEIMKKAKNELDTHIGHDRQVDEHDISKLPYLQSIVYE 369

Query: 380 TLRLFPAAPLLVPHESSEECRVEGFDIPKGTMLLVNAWAIHRDPRVWEDPTSFRPERFLN 439
           TLRL  AAPLLVPH SSE+  + G++IP+ T+L+VNAW IHRDP +W DPT F+PERF  
Sbjct: 370 TLRLHAAAPLLVPHLSSEDFSLGGYNIPQNTILMVNAWVIHRDPNLWSDPTCFKPERF-- 429

Query: 440 WETAESHKWVPFGVGRRACPGAALAHRVVGLTLATLIQCFEWQRVGEEPIDLSQGTGITT 499
            +  E +K + FG+GRRACPG  L+ R  GLTL  LIQCFEW+R+GEE ID+ +  GIT 
Sbjct: 430 EKEGEVNKLLSFGLGRRACPGENLSQRTEGLTLGLLIQCFEWKRIGEEKIDMVEAKGITA 489

Query: 500 PKAIPLEAMCRAR 510
            K   L AMC+ R
Sbjct: 490 GKKTSLNAMCKVR 490

[Close]
BLAST of CmaCh09G002670 vs. ExPASy Swiss-Prot
Match: P93147 (Isoflavone 2'-hydroxylase OS=Glycyrrhiza echinata OX=46348 GN=CYP81E1 PE=1 SV=2)

HSP 1 Score: 497.7 bits (1280), Expect = 1.6e-139
Identity = 245/499 (49.10%), Postives = 350/499 (70.14%), Query Frame = 0 

Query: 17  ITCLSLSLL-LTLILIYK--LRKQSPRNRPPSPPSLPLIGHLHLLKQPFHRVLQDLSNKH 76
           ++ LS S+  L L  I+   +R +  +N PP PPSLP+IG+LH LK+P HR  + LS K+
Sbjct: 4   LSLLSYSVFYLALFFIFNIVIRARKFKNLPPGPPSLPIIGNLHHLKRPLHRTFKGLSEKY 63

Query: 77  GPILSLSIGSRPLVVISSPTAVRECFTKNDIALANRPRLLAGKYMNYNYTGVAFASYGHH 136
           G + SL  GSR +VV+SS +  ++CFTKND+ LANRPR L+GKY+ YNYT +   SYG H
Sbjct: 64  GHVFSLWFGSRLVVVVSSASEFQQCFTKNDVVLANRPRFLSGKYIFYNYTTLGSTSYGEH 123

Query: 137 WRNMRRIATTELLSNHRLNAYLNIRVQEL-KLVKNLYGATGESSDFVRLEMQSKLNELSF 196
           WRN+RRI   ++LSNHR+N++  IR  E  +L+  L  A   S++F  +E+ S+L +++F
Sbjct: 124 WRNLRRITALDVLSNHRINSFSGIRRDETQRLITRL--ADDSSTNFAEMELSSRLYDMTF 183

Query: 197 NIVMRMVSGKRYFG--VDVEDVEEAREFREIMKELLQLGGASNPADFLPILRLFDYQGLE 256
           N +MRM+SGKRY+G   D  D++EA +FR+++ ELLQL GA+N  DF+P+LR  D++ LE
Sbjct: 184 NNIMRMISGKRYYGEDCDTSDLQEASQFRDMVSELLQLSGANNKTDFMPLLRFLDFENLE 243

Query: 257 KRMVQASGRADSFLQNLIDCERDRRASRWPEEKRGNKTMIQSLLSLQESQPQHYSDDIIK 316
           KR+   SG+ D+FL+ LI+  R ++       +R N TMI  LL+LQ+SQP++Y+D IIK
Sbjct: 244 KRLKDISGKTDAFLRGLIEEHRTKK-------ERAN-TMIDHLLNLQDSQPEYYTDQIIK 303

Query: 317 GHVLTMLAAGTDTTTGTLEWAMSLLLNHPAIMNKAWSEIRECVGESRLVEEGDVSSLKYL 376
           G  L ML AGTD++  TLEW+MS LLNHP ++ K   E+   VG+ RLV+E D+  L YL
Sbjct: 304 GLALAMLLAGTDSSAVTLEWSMSNLLNHPEVLKKVKDELDTHVGQDRLVDESDLPKLTYL 363

Query: 377 VAIIYETLRLFPAAPLLVPHESSEECRVEGFDIPKGTMLLVNAWAIHRDPRVWEDPTSFR 436
             +I ETLRL+  APLL+PH +S+EC + G+ +P+ T++L+NAWAIHRDP +W + T+F+
Sbjct: 364 KNVINETLRLYTPAPLLLPHSTSDECNIGGYKVPQDTIVLINAWAIHRDPELWTEATTFK 423

Query: 437 PERFLNWETAESHKWVPFGVGRRACPGAALAHRVVGLTLATLIQCFEWQRVGEEPIDLSQ 496
           PERF   +  E  K + FG+GRRACPG  LA R + +TLA LIQCF+W+ +  + IDL++
Sbjct: 424 PERF--EKKGELEKLIAFGMGRRACPGEGLAIRAISMTLALLIQCFDWKLINGDKIDLAE 483

Query: 497 GTGITTPKAIPLEAMCRAR 510
             G T  K +PL+AMC++R
Sbjct: 484 RDGFTLTKLVPLKAMCKSR 490

[Close]
BLAST of CmaCh09G002670 vs. ExPASy Swiss-Prot
Match: Q6WNR0 (Isoflavone 2'-hydroxylase OS=Medicago truncatula OX=3880 GN=CYP81E7 PE=1 SV=1)

HSP 1 Score: 482.6 bits (1241), Expect = 5.4e-135
Identity = 234/491 (47.66%), Postives = 340/491 (69.25%), Query Frame = 0 

Query: 22  LSLLLTLILIYKLRKQSPRNRPPSPPSLPLIGHLHLLKQPFHRVLQDLSNKHGPILSLSI 81
           LS+   + L+++ RK   +N PP P SLP+IG+LH LK+P +R  + L+ K+G ++SL  
Sbjct: 14  LSIFFIIRLLFQSRKF--KNLPPGPTSLPIIGNLHHLKRPLNRTFKALTEKYGNVISLWF 73

Query: 82  GSRPLVVISSPTAVRECFTKNDIALANRPRLLAGKYMNYNYTGVAFASYGHHWRNMRRIA 141
           GSR +VV+SS +  +ECFTKND+ LANRPR L+GKY+ YNYT +   SYG HWRN+RRI 
Sbjct: 74  GSRLVVVVSSLSEFQECFTKNDVVLANRPRFLSGKYIFYNYTTLGSTSYGEHWRNLRRIT 133

Query: 142 TTELLSNHRLNAYLNIRVQEL-KLVKNLYGATGESSDFVRLEMQSKLNELSFNIVMRMVS 201
           + ++LSNHR+N +  IR  E  +L+K L  A   S+ F  +E+  +  +++FN +MRM+S
Sbjct: 134 SLDVLSNHRINNFAPIRRDETQRLIKKL--AEDSSTKFAEVELTFRFFDMTFNNIMRMIS 193

Query: 202 GKRYFG--VDVEDVEEAREFREIMKELLQLGGASNPADFLPILRLFDYQGLEKRMVQASG 261
           GKRY+G   D+ +V+EA +FR+++ ELLQL GA+N  DF+P+L+  D++ LEKR+ +   
Sbjct: 194 GKRYYGDDCDISEVQEASQFRDMVSELLQLSGANNKTDFMPLLKFLDFENLEKRVKRIGE 253

Query: 262 RADSFLQNLIDCERDRRASRWPEEKRGNKTMIQSLLSLQESQPQHYSDDIIKGHVLTMLA 321
           + D FL  L+  +R +        K    TMI  LL++QESQP++Y+D IIKG  L ML 
Sbjct: 254 KNDVFLSGLLQEQRSK--------KERTNTMIDHLLNMQESQPEYYTDTIIKGLCLAMLL 313

Query: 322 AGTDTTTGTLEWAMSLLLNHPAIMNKAWSEIRECVGESRLVEEGDVSSLKYLVAIIYETL 381
           AGTD++  TLEW MS +LN+P ++ K   E+   VG+ RLV+E D+  L YL  +IYETL
Sbjct: 314 AGTDSSAVTLEWTMSNILNYPEVLKKVRDEVDTHVGQDRLVDESDLPKLTYLRNVIYETL 373

Query: 382 RLFPAAPLLVPHESSEECRVEGFDIPKGTMLLVNAWAIHRDPRVWEDPTSFRPERFLNWE 441
           RL+  APLL+PH +++EC + G+ +P+ T++L+NAWAIHRDP  W + T+F+PERF   +
Sbjct: 374 RLYTPAPLLLPHSTADECIMGGYKVPRDTIVLINAWAIHRDPETWSEATTFKPERF--DK 433

Query: 442 TAESHKWVPFGVGRRACPGAALAHRVVGLTLATLIQCFEWQRVGEEPIDLSQGTGITTPK 501
             E  K + FG+GRRACPG  LA R + +TLA L+QCF+W+R+ +E ID+S+  G T  K
Sbjct: 434 KGELEKMIAFGMGRRACPGEGLALRAISMTLALLVQCFDWKRINDEKIDMSERDGFTMTK 490

Query: 502 AIPLEAMCRAR 510
            +PL+AMC+ R
Sbjct: 494 LLPLKAMCKTR 490

[Close]
BLAST of CmaCh09G002670 vs. ExPASy TrEMBL
Match: A0A6J1IL26 (isoflavone 3'-hydroxylase-like OS=Cucurbita maxima OX=3661 GN=LOC111477203 PE=3 SV=1)

HSP 1 Score: 1046.6 bits (2705), Expect = 3.5e-302
Identity = 519/519 (100.00%), Postives = 519/519 (100.00%), Query Frame = 0 

Query: 1   MHLQQLPTYSSMEANTITCLSLSLLLTLILIYKLRKQSPRNRPPSPPSLPLIGHLHLLKQ 60
           MHLQQLPTYSSMEANTITCLSLSLLLTLILIYKLRKQSPRNRPPSPPSLPLIGHLHLLKQ
Sbjct: 1   MHLQQLPTYSSMEANTITCLSLSLLLTLILIYKLRKQSPRNRPPSPPSLPLIGHLHLLKQ 60

Query: 61  PFHRVLQDLSNKHGPILSLSIGSRPLVVISSPTAVRECFTKNDIALANRPRLLAGKYMNY 120
           PFHRVLQDLSNKHGPILSLSIGSRPLVVISSPTAVRECFTKNDIALANRPRLLAGKYMNY
Sbjct: 61  PFHRVLQDLSNKHGPILSLSIGSRPLVVISSPTAVRECFTKNDIALANRPRLLAGKYMNY 120

Query: 121 NYTGVAFASYGHHWRNMRRIATTELLSNHRLNAYLNIRVQELKLVKNLYGATGESSDFVR 180
           NYTGVAFASYGHHWRNMRRIATTELLSNHRLNAYLNIRVQELKLVKNLYGATGESSDFVR
Sbjct: 121 NYTGVAFASYGHHWRNMRRIATTELLSNHRLNAYLNIRVQELKLVKNLYGATGESSDFVR 180

Query: 181 LEMQSKLNELSFNIVMRMVSGKRYFGVDVEDVEEAREFREIMKELLQLGGASNPADFLPI 240
           LEMQSKLNELSFNIVMRMVSGKRYFGVDVEDVEEAREFREIMKELLQLGGASNPADFLPI
Sbjct: 181 LEMQSKLNELSFNIVMRMVSGKRYFGVDVEDVEEAREFREIMKELLQLGGASNPADFLPI 240

Query: 241 LRLFDYQGLEKRMVQASGRADSFLQNLIDCERDRRASRWPEEKRGNKTMIQSLLSLQESQ 300
           LRLFDYQGLEKRMVQASGRADSFLQNLIDCERDRRASRWPEEKRGNKTMIQSLLSLQESQ
Sbjct: 241 LRLFDYQGLEKRMVQASGRADSFLQNLIDCERDRRASRWPEEKRGNKTMIQSLLSLQESQ 300

Query: 301 PQHYSDDIIKGHVLTMLAAGTDTTTGTLEWAMSLLLNHPAIMNKAWSEIRECVGESRLVE 360
           PQHYSDDIIKGHVLTMLAAGTDTTTGTLEWAMSLLLNHPAIMNKAWSEIRECVGESRLVE
Sbjct: 301 PQHYSDDIIKGHVLTMLAAGTDTTTGTLEWAMSLLLNHPAIMNKAWSEIRECVGESRLVE 360

Query: 361 EGDVSSLKYLVAIIYETLRLFPAAPLLVPHESSEECRVEGFDIPKGTMLLVNAWAIHRDP 420
           EGDVSSLKYLVAIIYETLRLFPAAPLLVPHESSEECRVEGFDIPKGTMLLVNAWAIHRDP
Sbjct: 361 EGDVSSLKYLVAIIYETLRLFPAAPLLVPHESSEECRVEGFDIPKGTMLLVNAWAIHRDP 420

Query: 421 RVWEDPTSFRPERFLNWETAESHKWVPFGVGRRACPGAALAHRVVGLTLATLIQCFEWQR 480
           RVWEDPTSFRPERFLNWETAESHKWVPFGVGRRACPGAALAHRVVGLTLATLIQCFEWQR
Sbjct: 421 RVWEDPTSFRPERFLNWETAESHKWVPFGVGRRACPGAALAHRVVGLTLATLIQCFEWQR 480

Query: 481 VGEEPIDLSQGTGITTPKAIPLEAMCRARSSMLHLLSPL 520
           VGEEPIDLSQGTGITTPKAIPLEAMCRARSSMLHLLSPL
Sbjct: 481 VGEEPIDLSQGTGITTPKAIPLEAMCRARSSMLHLLSPL 519

[Close]
BLAST of CmaCh09G002670 vs. ExPASy TrEMBL
Match: A0A6J1F8L9 (isoflavone 3'-hydroxylase-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111443310 PE=3 SV=1)

HSP 1 Score: 957.2 bits (2473), Expect = 2.8e-275
Identity = 474/509 (93.12%), Postives = 487/509 (95.68%), Query Frame = 0 

Query: 12  MEANTITCLSLSLLLTLILIYKLRKQSPRNRPPSPPSLPLIGHLHLLKQPFHRVLQDLSN 71
           MEANTIT LSLSLL TL LIYKLRKQSPR  PPSPPSLPLIGHLHLLKQPFHRVLQDLS+
Sbjct: 1   MEANTITWLSLSLLFTLSLIYKLRKQSPRRCPPSPPSLPLIGHLHLLKQPFHRVLQDLSD 60

Query: 72  KHGPILSLSIGSRPLVVISSPTAVRECFTKNDIALANRPRLLAGKYMNYNYTGVAFASYG 131
           KHGPILSLSIGSRP+VVISSPTAVRECFTKNDIA ANRPRLL+GKYMNYNYTGVA ASYG
Sbjct: 61  KHGPILSLSIGSRPMVVISSPTAVRECFTKNDIAFANRPRLLSGKYMNYNYTGVAVASYG 120

Query: 132 HHWRNMRRIATTELLSNHRLNAYLNIRVQELKL-VKNLYGATGESSDFVRLEMQSKLNEL 191
           HHWRNMRRIATTELLSNHRLNAYLNIRVQELKL VKNLYG TGESSDFVRLEMQSKLNEL
Sbjct: 121 HHWRNMRRIATTELLSNHRLNAYLNIRVQELKLWVKNLYGETGESSDFVRLEMQSKLNEL 180

Query: 192 SFNIVMRMVSGKRYFGVDVEDVEEAREFREIMKELLQLGGASNPADFLPILRLFDYQGLE 251
           SFN VMRMVSGKRYFGVDVEDVEEAREFREIMKELL L GASNPADFLPILRLFDYQGLE
Sbjct: 181 SFNTVMRMVSGKRYFGVDVEDVEEAREFREIMKELLHLSGASNPADFLPILRLFDYQGLE 240

Query: 252 KRMVQASGRADSFLQNLIDCERDRRASRWPEEKRGNKTMIQSLLSLQESQPQHYSDDIIK 311
           KRMV+ASGRAD FLQNLIDCER+RRASRWPEEK GNKTMIQSLLS QESQPQ+YSDDIIK
Sbjct: 241 KRMVKASGRADLFLQNLIDCERERRASRWPEEKHGNKTMIQSLLSFQESQPQYYSDDIIK 300

Query: 312 GHVLTMLAAGTDTTTGTLEWAMSLLLNHPAIMNKAWSEIRECVGESRLVEEGDVSSLKYL 371
           GHVLTMLAAGTDTTTGT+EWAMSLLLNHP IM+KAWSEIRECVGESRLVEEGDVSSLKY+
Sbjct: 301 GHVLTMLAAGTDTTTGTIEWAMSLLLNHPTIMDKAWSEIRECVGESRLVEEGDVSSLKYV 360

Query: 372 VAIIYETLRLFPAAPLLVPHESSEECRVEGFDIPKGTMLLVNAWAIHRDPRVWEDPTSFR 431
            AIIYETLRLFP APLLVPHESSEECR+EGFDIPKGTMLLVNAWAIHRDP++WEDPTSFR
Sbjct: 361 EAIIYETLRLFPVAPLLVPHESSEECRIEGFDIPKGTMLLVNAWAIHRDPQLWEDPTSFR 420

Query: 432 PERFLNWETAESHKWVPFGVGRRACPGAALAHRVVGLTLATLIQCFEWQRVGEEPIDLSQ 491
           PERFLNWE AESHKWVPFGVGRRACPG ALAHRVVGLTLATLIQCFEWQRVGEEPIDLS+
Sbjct: 421 PERFLNWEAAESHKWVPFGVGRRACPGVALAHRVVGLTLATLIQCFEWQRVGEEPIDLSE 480

Query: 492 GTGITTPKAIPLEAMCRARSSMLHLLSPL 520
           GTGIT PKAIPL AMCRARSSMLHLLSPL
Sbjct: 481 GTGITMPKAIPLVAMCRARSSMLHLLSPL 509

[Close]
BLAST of CmaCh09G002670 vs. ExPASy TrEMBL
Match: A0A5A7VB12 (Cytochrome P450 81E8-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold134G002950 PE=3 SV=1)

HSP 1 Score: 785.4 bits (2027), Expect = 1.4e-223
Identity = 389/519 (74.95%), Postives = 451/519 (86.90%), Query Frame = 0 

Query: 8   TYSSMEANTITCLSLSLLL--TLILIYKLRK-QSPRNRPPSPPSLPLIGHLHLLKQPFHR 67
           TY SME +TIT  SLSLLL  TLIL+ KLRK ++ +  PP+PPSLP+IGHLHLLK+PFHR
Sbjct: 5   TYPSMEPHTITWFSLSLLLITTLILLSKLRKVRNQKKLPPTPPSLPIIGHLHLLKEPFHR 64

Query: 68  VLQDLSNKHGPILSLSIGSRPLVVISSPTAVRECFTKNDIALANRPRLLAGKYMNYNYTG 127
           VL +LSNK+GPILSL+IGSRP++V+SSPTAVRECFTKNDI  ANRPRL++GKY+NYNYT 
Sbjct: 65  VLHNLSNKYGPILSLTIGSRPVLVVSSPTAVRECFTKNDIVFANRPRLISGKYINYNYTA 124

Query: 128 VAFASYGHHWRNMRRIATTELLSNHRLNAYLNIRVQELKL-VKNLYGATGESSDFVRLEM 187
           V FA YG HWRNMRRIATTELLSNHRLN YLNIRV+ELKL VKN     G   DFV ++M
Sbjct: 125 VGFAPYGQHWRNMRRIATTELLSNHRLNMYLNIRVEELKLWVKNNLYKWGSGGDFVVVDM 184

Query: 188 QSKLNELSFNIVMRMVSGKRYFGVDVEDVEEAREFREIMKELLQLGGASNPADFLPILRL 247
           +SKL ELSFN VMRM+SGKRYFGV+VEDVEEA EFREIMKELL+L GA+N ADFL ILR+
Sbjct: 185 KSKLKELSFNTVMRMISGKRYFGVEVEDVEEAFEFREIMKELLELSGATNAADFLKILRV 244

Query: 248 FDYQGLEKRMVQASGRADSFLQNLIDCERDRRASRWPEE--KRGNKTMIQSLLSLQESQP 307
           FD +G++KRM++ASGRAD FLQ LID ER +R SRWPEE  K+   +MI++LLSLQESQP
Sbjct: 245 FDLEGVKKRMMKASGRADVFLQKLIDEERKKRVSRWPEEKQKQAKTSMIRTLLSLQESQP 304

Query: 308 QHYSDDIIKGHVLTMLAAGTDTTTGTLEWAMSLLLNHPAIMNKAWSEIRECVGESRLVEE 367
           Q+YSDDIIKGHVLTMLAAGTDTT+GT+EWAMSLLLNHP  M KAW EIR+CVGE+++VEE
Sbjct: 305 QYYSDDIIKGHVLTMLAAGTDTTSGTIEWAMSLLLNHPMTMKKAWIEIRDCVGENQMVEE 364

Query: 368 GDVSSLKYLVAIIYETLRLFPAAPLLVPHESSEECRVEGFDIPKGTMLLVNAWAIHRDPR 427
           GDVS LKYL AIIYETLR+FPAAPLLVPHE SE+C +EGF++PKGTMLLVNAW IHRDP+
Sbjct: 365 GDVSELKYLEAIIYETLRMFPAAPLLVPHECSEDCSIEGFEVPKGTMLLVNAWGIHRDPK 424

Query: 428 VWEDPTSFRPERFLNWETAESHKWVPFGVGRRACPGAALAHRVVGLTLATLIQCFEWQRV 487
           VWEDPTSF+PERFLNWE  E++KW+PFG+GRRACPG ALA R +GLTLATLIQCFEW++V
Sbjct: 425 VWEDPTSFQPERFLNWEGVETYKWIPFGMGRRACPGVALAQRSMGLTLATLIQCFEWKKV 484

Query: 488 GE-EPIDLSQGTGITTPKAIPLEAMCRARSSMLHLLSPL 520
            E E IDLS+G+GIT PKA  LEAMC+ R+SMLHLL+ L
Sbjct: 485 DEQEQIDLSEGSGITMPKAKALEAMCKPRTSMLHLLAQL 523

[Close]
BLAST of CmaCh09G002670 vs. ExPASy TrEMBL
Match: A0A1S3BWG9 (LOW QUALITY PROTEIN: cytochrome P450 81E8-like OS=Cucumis melo OX=3656 GN=LOC103493891 PE=3 SV=1)

HSP 1 Score: 777.3 bits (2006), Expect = 3.9e-221
Identity = 387/519 (74.57%), Postives = 450/519 (86.71%), Query Frame = 0 

Query: 8   TYSSMEANTITCLSLSLLL--TLILIYKLRK-QSPRNRPPSPPSLPLIGHLHLLKQPFHR 67
           TY SME +TIT  SLSLLL  TLIL+ KLRK ++ +  PP+PPSLP+IGHLHLLK+PFHR
Sbjct: 2   TYPSMEPHTITWFSLSLLLITTLILLSKLRKVRNQKKLPPTPPSLPIIGHLHLLKEPFHR 61

Query: 68  VLQDLSNKHGPILSLSIGSRPLVVISSPTAVRECFTKNDIALANRPRLLAGKYMNYNYTG 127
           VL +LSNK+GPILSL+IGSRP++V+SSPTAVRECFTKNDI  ANRPRL++GKY+NYNYT 
Sbjct: 62  VLHNLSNKYGPILSLTIGSRPVLVVSSPTAVRECFTKNDIVFANRPRLISGKYINYNYTA 121

Query: 128 VAFASYGHHWRNMRRIATTELLSNHRLNAYLNIRVQELKL-VKNLYGATGESSDFVRLEM 187
           V FA YG HWRNMRRIATTELLSNHRLN YLNIRV+ELKL VKN     G   DFV ++M
Sbjct: 122 VGFAPYGQHWRNMRRIATTELLSNHRLNMYLNIRVEELKLWVKNNLYKWGSGGDFVVVDM 181

Query: 188 QSKLNELSFNIVMRMVSGKRYFGVDVEDVEEAREFREIMKELLQLGGASNPADFLPILRL 247
           +SKL ELSFN VMRM+SGKRYFGV+VEDVEEA EFREIMKELL+L GA+N ADFL ILR+
Sbjct: 182 KSKLKELSFNTVMRMISGKRYFGVEVEDVEEAFEFREIMKELLELSGATNAADFLKILRV 241

Query: 248 FDYQGLEKRMVQASGRADSFLQNLIDCERDRRASRWPEE--KRGNKTMIQSLLSLQESQP 307
           FD +G++KRM++ASGRAD FLQ LID ER ++  RWPEE  K+   +MI++LLSLQESQP
Sbjct: 242 FDLEGVKKRMMKASGRADVFLQKLIDEER-KKEFRWPEEKQKQAKTSMIRTLLSLQESQP 301

Query: 308 QHYSDDIIKGHVLTMLAAGTDTTTGTLEWAMSLLLNHPAIMNKAWSEIRECVGESRLVEE 367
           Q+YSDDIIKGHVLTMLAAGTDTT+GT+EWAMSLLLNHP  M KAW EIR+CVGE+++VEE
Sbjct: 302 QYYSDDIIKGHVLTMLAAGTDTTSGTIEWAMSLLLNHPMTMKKAWIEIRDCVGENQMVEE 361

Query: 368 GDVSSLKYLVAIIYETLRLFPAAPLLVPHESSEECRVEGFDIPKGTMLLVNAWAIHRDPR 427
           GDVS LKYL AIIYETLR+FPAAPLLVPHE SE+C +EGF++PKGTMLLVNAW IHRDP+
Sbjct: 362 GDVSELKYLEAIIYETLRMFPAAPLLVPHECSEDCSIEGFEVPKGTMLLVNAWGIHRDPK 421

Query: 428 VWEDPTSFRPERFLNWETAESHKWVPFGVGRRACPGAALAHRVVGLTLATLIQCFEWQRV 487
           VWEDPTSF+PERFLNWE  E++KW+PFG+GRRACPG ALA R +GLTLATLIQCFEW++V
Sbjct: 422 VWEDPTSFQPERFLNWEGVETYKWIPFGMGRRACPGVALAQRSMGLTLATLIQCFEWKKV 481

Query: 488 GE-EPIDLSQGTGITTPKAIPLEAMCRARSSMLHLLSPL 520
            E E IDLS+G+GIT PKA  LEAMC+ R+SMLHLL+ L
Sbjct: 482 DEQEQIDLSEGSGITMPKAKALEAMCKPRTSMLHLLAQL 519

[Close]
BLAST of CmaCh09G002670 vs. ExPASy TrEMBL
Match: A0A6J1C9L7 (isoflavone 2'-hydroxylase-like OS=Momordica charantia OX=3673 GN=LOC111009467 PE=3 SV=1)

HSP 1 Score: 649.4 bits (1674), Expect = 1.2e-182
Identity = 314/503 (62.43%), Postives = 399/503 (79.32%), Query Frame = 0 

Query: 20  LSLSLLLTLILI--YKLRKQSPRNRPPSPPSLPLIGHLHLLKQPFHRVLQDLSNKHGPIL 79
           + L+LL  LI +  +KL+K S +N PP PPSLP+IGHLHLL++PFH++LQ+LS K+GPIL
Sbjct: 12  IPLALLFALIHVFNFKLKKVSSKNLPPGPPSLPVIGHLHLLREPFHQMLQNLSKKYGPIL 71

Query: 80  SLSIGSRPLVVISSPTAVRECFTKNDIALANRPRLLAGKYMNYNYTGVAFASYGHHWRNM 139
           SLS+G R +VV+SSP+A +ECFTKNDI  ANRPR+++GK +NYNYT V   +YG HWR M
Sbjct: 72  SLSLGFRSVVVVSSPSAAQECFTKNDIVFANRPRMISGKILNYNYTSVGATAYGQHWRIM 131

Query: 140 RRIATTELLSNHRLNAYLNIRVQELKL-VKNLYGATGESSDFVRLEMQSKLNELSFNIVM 199
           RRIATT+LLS HRLN+++N+R QE+KL +KNLY  TG+++  V++E+++KL ELSFNI +
Sbjct: 132 RRIATTDLLSTHRLNSFINLRQQEVKLWLKNLYQTTGQNN--VQVEIRTKLTELSFNIAL 191

Query: 200 RMVSGKRYFGVDVEDVEEAREFREIMKELLQLGGASNPADFLPILRLFDYQGLEKRMVQA 259
           RM++GKRYFGVDVED EE R+ +++MKE+  L GAS PADFL +L+L DYQG  KR+ + 
Sbjct: 192 RMMTGKRYFGVDVEDSEEGRKLKKVMKEVSLLSGASYPADFLSVLKLIDYQGFRKRLWKV 251

Query: 260 SGRADSFLQNLIDCERDRRASRWPEEKRGNKTMIQSLLSLQESQPQHYSDDIIKGHVLTM 319
              AD F Q+LID  R+R+AS     +   K MI ++LSLQ+S P +Y D IIKG +LT+
Sbjct: 252 WAGADGFAQSLIDGRRERKASCSFSPEESRKNMIDTMLSLQDSDPGYYKDHIIKGQILTL 311

Query: 320 LAAGTDTTTGTLEWAMSLLLNHPAIMNKAWSEIRECVGESRLVEEGDVSSLKYLVAIIYE 379
           LAAGTDTT GT+EW MSLLLNH  IM K WSEI E VGE RLVEE D+++L +L AII E
Sbjct: 312 LAAGTDTTAGTIEWGMSLLLNHRTIMKKVWSEINEYVGEQRLVEEADLTNLPFLQAIINE 371

Query: 380 TLRLFPAAPLLVPHESSEECRVEGFDIPKGTMLLVNAWAIHRDPRVWEDPTSFRPERFLN 439
           T RLFPA P+LVP ESSE+C + G+DIPKGTML+VN WAIHRDP+VW+DPTSF+PERF N
Sbjct: 372 TNRLFPALPILVPRESSEDCTIGGYDIPKGTMLVVNVWAIHRDPKVWQDPTSFQPERFEN 431

Query: 440 WETAESHKWVPFGVGRRACPGAALAHRVVGLTLATLIQCFEWQRVGEEPIDLSQGTGITT 499
            E  E++K +PFG+GRRACPG+ LAHRVVGL LATLIQCFEW ++G   IDL QG G+T 
Sbjct: 432 GEVVEANKLLPFGMGRRACPGSGLAHRVVGLALATLIQCFEWDKIGPNEIDLFQGNGLTM 491

Query: 500 PKAIPLEAMCRARSSMLHLLSPL 520
           PKA PLEAMC+ R+ M++LLS L
Sbjct: 492 PKAHPLEAMCKPRAPMINLLSQL 512

[Close]
BLAST of CmaCh09G002670 vs. NCBI nr
Match: XP_022977005.1 (isoflavone 3'-hydroxylase-like [Cucurbita maxima])

HSP 1 Score: 1046.6 bits (2705), Expect = 7.2e-302
Identity = 519/519 (100.00%), Postives = 519/519 (100.00%), Query Frame = 0 

Query: 1   MHLQQLPTYSSMEANTITCLSLSLLLTLILIYKLRKQSPRNRPPSPPSLPLIGHLHLLKQ 60
           MHLQQLPTYSSMEANTITCLSLSLLLTLILIYKLRKQSPRNRPPSPPSLPLIGHLHLLKQ
Sbjct: 1   MHLQQLPTYSSMEANTITCLSLSLLLTLILIYKLRKQSPRNRPPSPPSLPLIGHLHLLKQ 60

Query: 61  PFHRVLQDLSNKHGPILSLSIGSRPLVVISSPTAVRECFTKNDIALANRPRLLAGKYMNY 120
           PFHRVLQDLSNKHGPILSLSIGSRPLVVISSPTAVRECFTKNDIALANRPRLLAGKYMNY
Sbjct: 61  PFHRVLQDLSNKHGPILSLSIGSRPLVVISSPTAVRECFTKNDIALANRPRLLAGKYMNY 120

Query: 121 NYTGVAFASYGHHWRNMRRIATTELLSNHRLNAYLNIRVQELKLVKNLYGATGESSDFVR 180
           NYTGVAFASYGHHWRNMRRIATTELLSNHRLNAYLNIRVQELKLVKNLYGATGESSDFVR
Sbjct: 121 NYTGVAFASYGHHWRNMRRIATTELLSNHRLNAYLNIRVQELKLVKNLYGATGESSDFVR 180

Query: 181 LEMQSKLNELSFNIVMRMVSGKRYFGVDVEDVEEAREFREIMKELLQLGGASNPADFLPI 240
           LEMQSKLNELSFNIVMRMVSGKRYFGVDVEDVEEAREFREIMKELLQLGGASNPADFLPI
Sbjct: 181 LEMQSKLNELSFNIVMRMVSGKRYFGVDVEDVEEAREFREIMKELLQLGGASNPADFLPI 240

Query: 241 LRLFDYQGLEKRMVQASGRADSFLQNLIDCERDRRASRWPEEKRGNKTMIQSLLSLQESQ 300
           LRLFDYQGLEKRMVQASGRADSFLQNLIDCERDRRASRWPEEKRGNKTMIQSLLSLQESQ
Sbjct: 241 LRLFDYQGLEKRMVQASGRADSFLQNLIDCERDRRASRWPEEKRGNKTMIQSLLSLQESQ 300

Query: 301 PQHYSDDIIKGHVLTMLAAGTDTTTGTLEWAMSLLLNHPAIMNKAWSEIRECVGESRLVE 360
           PQHYSDDIIKGHVLTMLAAGTDTTTGTLEWAMSLLLNHPAIMNKAWSEIRECVGESRLVE
Sbjct: 301 PQHYSDDIIKGHVLTMLAAGTDTTTGTLEWAMSLLLNHPAIMNKAWSEIRECVGESRLVE 360

Query: 361 EGDVSSLKYLVAIIYETLRLFPAAPLLVPHESSEECRVEGFDIPKGTMLLVNAWAIHRDP 420
           EGDVSSLKYLVAIIYETLRLFPAAPLLVPHESSEECRVEGFDIPKGTMLLVNAWAIHRDP
Sbjct: 361 EGDVSSLKYLVAIIYETLRLFPAAPLLVPHESSEECRVEGFDIPKGTMLLVNAWAIHRDP 420

Query: 421 RVWEDPTSFRPERFLNWETAESHKWVPFGVGRRACPGAALAHRVVGLTLATLIQCFEWQR 480
           RVWEDPTSFRPERFLNWETAESHKWVPFGVGRRACPGAALAHRVVGLTLATLIQCFEWQR
Sbjct: 421 RVWEDPTSFRPERFLNWETAESHKWVPFGVGRRACPGAALAHRVVGLTLATLIQCFEWQR 480

Query: 481 VGEEPIDLSQGTGITTPKAIPLEAMCRARSSMLHLLSPL 520
           VGEEPIDLSQGTGITTPKAIPLEAMCRARSSMLHLLSPL
Sbjct: 481 VGEEPIDLSQGTGITTPKAIPLEAMCRARSSMLHLLSPL 519

[Close]
BLAST of CmaCh09G002670 vs. NCBI nr
Match: XP_023535658.1 (isoflavone 2'-hydroxylase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 981.1 bits (2535), Expect = 3.7e-282
Identity = 486/520 (93.46%), Postives = 501/520 (96.35%), Query Frame = 0 

Query: 1   MHLQQLPTYSSMEANTITCLSLSLLLTLILIYKLRKQSPRNRPPSPPSLPLIGHLHLLKQ 60
           MHLQQLP YSSMEA+TIT LSLSLL TLILI+KLRKQSPR  PPSPPSLPLIGHLHLLKQ
Sbjct: 9   MHLQQLPIYSSMEASTITWLSLSLLFTLILIHKLRKQSPRKCPPSPPSLPLIGHLHLLKQ 68

Query: 61  PFHRVLQDLSNKHGPILSLSIGSRPLVVISSPTAVRECFTKNDIALANRPRLLAGKYMNY 120
           PFHRVLQDLSNKHGPILSLSIGSRP+VVISSPTAVRECFTKNDIA ANRPRLL+GKYMNY
Sbjct: 69  PFHRVLQDLSNKHGPILSLSIGSRPMVVISSPTAVRECFTKNDIAFANRPRLLSGKYMNY 128

Query: 121 NYTGVAFASYGHHWRNMRRIATTELLSNHRLNAYLNIRVQELKL-VKNLYGATGESSDFV 180
           NYTGVAFASYGHHWRNMRRIATTELLSNHRLN YLNIRVQELKL VKNLYGATGESSDFV
Sbjct: 129 NYTGVAFASYGHHWRNMRRIATTELLSNHRLNTYLNIRVQELKLWVKNLYGATGESSDFV 188

Query: 181 RLEMQSKLNELSFNIVMRMVSGKRYFGVDVEDVEEAREFREIMKELLQLGGASNPADFLP 240
           RLEMQSKLNELSFN VMRMVSGKRYFGVDVEDVEEAREFREIMKELLQL GASNPADFLP
Sbjct: 189 RLEMQSKLNELSFNTVMRMVSGKRYFGVDVEDVEEAREFREIMKELLQLSGASNPADFLP 248

Query: 241 ILRLFDYQGLEKRMVQASGRADSFLQNLIDCERDRRASRWPEEKRGNKTMIQSLLSLQES 300
           ILRLFDYQGLEKRMV+ASGRAD FLQNLIDCER+RRAS WPEEK GNKTMIQSLLSLQES
Sbjct: 249 ILRLFDYQGLEKRMVKASGRADLFLQNLIDCERERRASPWPEEKHGNKTMIQSLLSLQES 308

Query: 301 QPQHYSDDIIKGHVLTMLAAGTDTTTGTLEWAMSLLLNHPAIMNKAWSEIRECVGESRLV 360
           QPQ+YSDDIIKGHVLTMLAAGTDTTTGT+EWAMSLLLNHP IMNKAWSEIRECVGESRLV
Sbjct: 309 QPQYYSDDIIKGHVLTMLAAGTDTTTGTIEWAMSLLLNHPTIMNKAWSEIRECVGESRLV 368

Query: 361 EEGDVSSLKYLVAIIYETLRLFPAAPLLVPHESSEECRVEGFDIPKGTMLLVNAWAIHRD 420
           EEGDVSSLKY+ AIIYETLRLFP APLLVPHESSEECR+EGFDIPKGTMLLVNAWAIHRD
Sbjct: 369 EEGDVSSLKYVEAIIYETLRLFPVAPLLVPHESSEECRIEGFDIPKGTMLLVNAWAIHRD 428

Query: 421 PRVWEDPTSFRPERFLNWETAESHKWVPFGVGRRACPGAALAHRVVGLTLATLIQCFEWQ 480
           P++WEDPTSFRPER LNWE+AESHKWVPFGVGRRACPGAALAHRVVGLTLATLIQCFEWQ
Sbjct: 429 PQLWEDPTSFRPERLLNWESAESHKWVPFGVGRRACPGAALAHRVVGLTLATLIQCFEWQ 488

Query: 481 RVGEEPIDLSQGTGITTPKAIPLEAMCRARSSMLHLLSPL 520
           RVGEEP+DLS+GTGIT PKAIPLEAMCRARSSMLHLLS L
Sbjct: 489 RVGEEPVDLSEGTGITIPKAIPLEAMCRARSSMLHLLSLL 528

[Close]
BLAST of CmaCh09G002670 vs. NCBI nr
Match: XP_022936846.1 (isoflavone 3'-hydroxylase-like isoform X1 [Cucurbita moschata] >XP_022936847.1 isoflavone 3'-hydroxylase-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 957.2 bits (2473), Expect = 5.7e-275
Identity = 474/509 (93.12%), Postives = 487/509 (95.68%), Query Frame = 0 

Query: 12  MEANTITCLSLSLLLTLILIYKLRKQSPRNRPPSPPSLPLIGHLHLLKQPFHRVLQDLSN 71
           MEANTIT LSLSLL TL LIYKLRKQSPR  PPSPPSLPLIGHLHLLKQPFHRVLQDLS+
Sbjct: 1   MEANTITWLSLSLLFTLSLIYKLRKQSPRRCPPSPPSLPLIGHLHLLKQPFHRVLQDLSD 60

Query: 72  KHGPILSLSIGSRPLVVISSPTAVRECFTKNDIALANRPRLLAGKYMNYNYTGVAFASYG 131
           KHGPILSLSIGSRP+VVISSPTAVRECFTKNDIA ANRPRLL+GKYMNYNYTGVA ASYG
Sbjct: 61  KHGPILSLSIGSRPMVVISSPTAVRECFTKNDIAFANRPRLLSGKYMNYNYTGVAVASYG 120

Query: 132 HHWRNMRRIATTELLSNHRLNAYLNIRVQELKL-VKNLYGATGESSDFVRLEMQSKLNEL 191
           HHWRNMRRIATTELLSNHRLNAYLNIRVQELKL VKNLYG TGESSDFVRLEMQSKLNEL
Sbjct: 121 HHWRNMRRIATTELLSNHRLNAYLNIRVQELKLWVKNLYGETGESSDFVRLEMQSKLNEL 180

Query: 192 SFNIVMRMVSGKRYFGVDVEDVEEAREFREIMKELLQLGGASNPADFLPILRLFDYQGLE 251
           SFN VMRMVSGKRYFGVDVEDVEEAREFREIMKELL L GASNPADFLPILRLFDYQGLE
Sbjct: 181 SFNTVMRMVSGKRYFGVDVEDVEEAREFREIMKELLHLSGASNPADFLPILRLFDYQGLE 240

Query: 252 KRMVQASGRADSFLQNLIDCERDRRASRWPEEKRGNKTMIQSLLSLQESQPQHYSDDIIK 311
           KRMV+ASGRAD FLQNLIDCER+RRASRWPEEK GNKTMIQSLLS QESQPQ+YSDDIIK
Sbjct: 241 KRMVKASGRADLFLQNLIDCERERRASRWPEEKHGNKTMIQSLLSFQESQPQYYSDDIIK 300

Query: 312 GHVLTMLAAGTDTTTGTLEWAMSLLLNHPAIMNKAWSEIRECVGESRLVEEGDVSSLKYL 371
           GHVLTMLAAGTDTTTGT+EWAMSLLLNHP IM+KAWSEIRECVGESRLVEEGDVSSLKY+
Sbjct: 301 GHVLTMLAAGTDTTTGTIEWAMSLLLNHPTIMDKAWSEIRECVGESRLVEEGDVSSLKYV 360

Query: 372 VAIIYETLRLFPAAPLLVPHESSEECRVEGFDIPKGTMLLVNAWAIHRDPRVWEDPTSFR 431
            AIIYETLRLFP APLLVPHESSEECR+EGFDIPKGTMLLVNAWAIHRDP++WEDPTSFR
Sbjct: 361 EAIIYETLRLFPVAPLLVPHESSEECRIEGFDIPKGTMLLVNAWAIHRDPQLWEDPTSFR 420

Query: 432 PERFLNWETAESHKWVPFGVGRRACPGAALAHRVVGLTLATLIQCFEWQRVGEEPIDLSQ 491
           PERFLNWE AESHKWVPFGVGRRACPG ALAHRVVGLTLATLIQCFEWQRVGEEPIDLS+
Sbjct: 421 PERFLNWEAAESHKWVPFGVGRRACPGVALAHRVVGLTLATLIQCFEWQRVGEEPIDLSE 480

Query: 492 GTGITTPKAIPLEAMCRARSSMLHLLSPL 520
           GTGIT PKAIPL AMCRARSSMLHLLSPL
Sbjct: 481 GTGITMPKAIPLVAMCRARSSMLHLLSPL 509

[Close]
BLAST of CmaCh09G002670 vs. NCBI nr
Match: KAG7024282.1 (Cytochrome P450 81E8, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 946.0 bits (2444), Expect = 1.3e-271
Identity = 470/509 (92.34%), Postives = 483/509 (94.89%), Query Frame = 0 

Query: 12  MEANTITCLSLSLLLTLILIYKLRKQSPRNRPPSPPSLPLIGHLHLLKQPFHRVLQDLSN 71
           MEANTIT LSLSLL TL LIYKLRKQSPR  PPSPPSLPLIGHLHLLKQPFHRVLQDLSN
Sbjct: 1   MEANTITWLSLSLLFTLSLIYKLRKQSPRRCPPSPPSLPLIGHLHLLKQPFHRVLQDLSN 60

Query: 72  KHGPILSLSIGSRPLVVISSPTAVRECFTKNDIALANRPRLLAGKYMNYNYTGVAFASYG 131
           KHGPILSLSIGSRPLVVISSPTAVRECFTKNDIA ANRPRLL+G YMNYNYTGVA ASYG
Sbjct: 61  KHGPILSLSIGSRPLVVISSPTAVRECFTKNDIAFANRPRLLSGNYMNYNYTGVAVASYG 120

Query: 132 HHWRNMRRIATTELLSNHRLNAYLNIRVQELKL-VKNLYGATGESSDFVRLEMQSKLNEL 191
           HHWRNMRR+AT ELLSN RL+ YLNIRVQELKL VKNLYGATGESSDFVRLEMQSKLNEL
Sbjct: 121 HHWRNMRRVATKELLSNQRLDTYLNIRVQELKLCVKNLYGATGESSDFVRLEMQSKLNEL 180

Query: 192 SFNIVMRMVSGKRYFGVDVEDVEEAREFREIMKELLQLGGASNPADFLPILRLFDYQGLE 251
           SFN VMRMVSGKRYFGVDVEDVEEAREFREIMKELLQL GASNPADFLPILRLFDYQGLE
Sbjct: 181 SFNTVMRMVSGKRYFGVDVEDVEEAREFREIMKELLQLSGASNPADFLPILRLFDYQGLE 240

Query: 252 KRMVQASGRADSFLQNLIDCERDRRASRWPEEKRGNKTMIQSLLSLQESQPQHYSDDIIK 311
           KRMV+AS RAD FLQNLIDCER+RR+SRWPEEK GNKTMIQSLLS QESQPQ+YSDDIIK
Sbjct: 241 KRMVKASSRADLFLQNLIDCERERRSSRWPEEKHGNKTMIQSLLSFQESQPQYYSDDIIK 300

Query: 312 GHVLTMLAAGTDTTTGTLEWAMSLLLNHPAIMNKAWSEIRECVGESRLVEEGDVSSLKYL 371
           GHVLTMLAAGTDTTTGT+EWAMSLLLNHP IMNKAWSEIRECVGESRLVEEGDVSSLKY+
Sbjct: 301 GHVLTMLAAGTDTTTGTIEWAMSLLLNHPTIMNKAWSEIRECVGESRLVEEGDVSSLKYV 360

Query: 372 VAIIYETLRLFPAAPLLVPHESSEECRVEGFDIPKGTMLLVNAWAIHRDPRVWEDPTSFR 431
            AIIYETLRLFP APLLVPHESSEECR+EGFDIPKGTMLLVNAWAIHRDP++WEDPTSFR
Sbjct: 361 EAIIYETLRLFPVAPLLVPHESSEECRIEGFDIPKGTMLLVNAWAIHRDPQLWEDPTSFR 420

Query: 432 PERFLNWETAESHKWVPFGVGRRACPGAALAHRVVGLTLATLIQCFEWQRVGEEPIDLSQ 491
           PERFLNWE AESHKWV FGVGR ACPGAALAHRVVGLTLATLIQCFEWQRVGEEPIDLS+
Sbjct: 421 PERFLNWEAAESHKWVLFGVGRGACPGAALAHRVVGLTLATLIQCFEWQRVGEEPIDLSE 480

Query: 492 GTGITTPKAIPLEAMCRARSSMLHLLSPL 520
           GTGIT PKAIPL AMCRARSSMLHLLSPL
Sbjct: 481 GTGITMPKAIPLVAMCRARSSMLHLLSPL 509

[Close]
BLAST of CmaCh09G002670 vs. NCBI nr
Match: XP_038896686.1 (cytochrome P450 81Q32-like [Benincasa hispida])

HSP 1 Score: 794.7 bits (2051), Expect = 4.9e-226
Identity = 391/513 (76.22%), Postives = 445/513 (86.74%), Query Frame = 0 

Query: 12  MEANTIT----CLSLSLLLTLILIYKLRKQSPRNRPPSPPSLPLIGHLHLLKQPFHRVLQ 71
           ME +TIT     L    L T IL+ KLRK + +  PPSPPSLP+IGHLHLL+ P HRVL 
Sbjct: 1   MEPHTITWFFPFLISLFLFTFILLSKLRKINHKKLPPSPPSLPVIGHLHLLRDPLHRVLH 60

Query: 72  DLSNKHGPILSLSIGSRPLVVISSPTAVRECFTKNDIALANRPRLLAGKYMNYNYTGVAF 131
           +LSNK+GPILSLSIGSRP+VV+SSPTAVRECFTKNDI  ANRPRLL+GKY+NYNYT V F
Sbjct: 61  NLSNKYGPILSLSIGSRPVVVVSSPTAVRECFTKNDIVFANRPRLLSGKYINYNYTAVGF 120

Query: 132 ASYGHHWRNMRRIATTELLSNHRLNAYLNIRVQELKL-VKNLYGATGESSDFVRLEMQSK 191
           A YG HWRNMRRIATTELLSNHRLN YLNIRVQELKL +KNLY       +FV +EM+SK
Sbjct: 121 APYGQHWRNMRRIATTELLSNHRLNTYLNIRVQELKLWIKNLYKLICREDNFVGVEMKSK 180

Query: 192 LNELSFNIVMRMVSGKRYFGVDVEDVEEAREFREIMKELLQLGGASNPADFLPILRLFDY 251
           L ELSFNIVMRMVSGKRYFGV+V DVEEA EFRE+ KELL+L GA+NPADFL ILR+FD+
Sbjct: 181 LKELSFNIVMRMVSGKRYFGVEVVDVEEALEFRELTKELLELSGATNPADFLTILRVFDF 240

Query: 252 QGLEKRMVQASGRADSFLQNLIDCERDRRASRWPEEKRGNKTMIQSLLSLQESQPQHYSD 311
           QGL KRM++ASGRAD FLQ LID +R+RR SRWPE+K G K+MIQ+LLSLQESQP +YSD
Sbjct: 241 QGLNKRMLKASGRADVFLQKLIDEQRERRLSRWPEDKHG-KSMIQTLLSLQESQPDYYSD 300

Query: 312 DIIKGHVLTMLAAGTDTTTGTLEWAMSLLLNHPAIMNKAWSEIRECVGESRLVEEGDVSS 371
           DIIKGHVLTMLAAGTDTT+GT+EW MSLLLNHP +M KAW EIR+CVGE+R+VEE DVS+
Sbjct: 301 DIIKGHVLTMLAAGTDTTSGTIEWTMSLLLNHPTVMKKAWIEIRDCVGENRMVEEADVSN 360

Query: 372 LKYLVAIIYETLRLFPAAPLLVPHESSEECRVEGFDIPKGTMLLVNAWAIHRDPRVWEDP 431
           L+Y+ AIIYETLRLFPAAPLLVPHESSE+CR+EGF++PKGTMLLVNAWAIHRDP+VWEDP
Sbjct: 361 LRYMEAIIYETLRLFPAAPLLVPHESSEDCRIEGFEVPKGTMLLVNAWAIHRDPKVWEDP 420

Query: 432 TSFRPERFLNWETAESHKWVPFGVGRRACPGAALAHRVVGLTLATLIQCFEWQRVGEEPI 491
            SFRPERFLNWE  ES+KW+PFG+GRRACPGAALA RVVGLTLATLIQCFEW++V E+ I
Sbjct: 421 NSFRPERFLNWEGVESYKWIPFGMGRRACPGAALAQRVVGLTLATLIQCFEWEKVDEQQI 480

Query: 492 DLSQGTGITTPKAIPLEAMCRARSSMLHLLSPL 520
           DLS+G+GIT PKA  L AMC+ R+SMLHLLS L
Sbjct: 481 DLSEGSGITMPKAKALVAMCKPRNSMLHLLSCL 512

[Close]
BLAST of CmaCh09G002670 vs. TAIR 10
Match: AT4G37370.1 (cytochrome P450, family 81, subfamily D, polypeptide 8 )

HSP 1 Score: 508.8 bits (1309), Expect = 5.0e-144
Identity = 259/511 (50.68%), Postives = 364/511 (71.23%), Query Frame = 0 

Query: 12  MEANTITCLSLSLLLTLILIYKLRKQSPRNRPPSPP-SLPLIGHLHLLKQPFHRVLQDLS 71
           ME  T+    L ++L+LI +    K+ P N PPSP  SLP+IGHL LLK P HR    LS
Sbjct: 1   METKTLIFSILFVVLSLIYLIGKLKRKP-NLPPSPAWSLPVIGHLRLLKPPIHRTFLSLS 60

Query: 72  N--KHGPILSLSIGSRPLVVISSPTAVRECFTKNDIALANRPRLLAGKYMNYNYTGVAFA 131
               + PI SL +G+R + V SS +   ECFTKND+ LANRP  +  K++ Y+YT +  A
Sbjct: 61  QSLNNAPIFSLRLGNRLVFVNSSHSIAEECFTKNDVVLANRPNFILAKHVAYDYTTMIAA 120

Query: 132 SYGHHWRNMRRIATTELLSNHRLNAYLNIRVQEL-KLVKNLYGATGESSDFVRLEMQSKL 191
           SYG HWRN+RRI + E+ SNHRLN++L+IR  E+ +LV  L  +   S +FV+++M+S L
Sbjct: 121 SYGDHWRNLRRIGSVEIFSNHRLNSFLSIRKDEIRRLVFRL--SRNFSQEFVKVDMKSML 180

Query: 192 NELSFNIVMRMVSGKRYFGVDVEDVEEAREFREIMKELLQLGGASNPADFLPILRLF-DY 251
           ++L+FN ++RMV+GKRY+G  VED  EA+  R+++ +++   GA N  D+LP+LRL  DY
Sbjct: 181 SDLTFNNILRMVAGKRYYGDGVEDDPEAKRVRQLIADVVACAGAGNAVDYLPVLRLVSDY 240

Query: 252 QGLEKRMVQASGRADSFLQNLIDCERDRRASRWPEEKRGNKTMIQSLLSLQESQPQHYSD 311
              E R+ + +GR D FLQ L+D +R        E K    TMI  LL+LQESQP +++D
Sbjct: 241 ---ETRVKKLAGRLDEFLQGLVDEKR--------EAKEKGNTMIDHLLTLQESQPDYFTD 300

Query: 312 DIIKGHVLTMLAAGTDTTTGTLEWAMSLLLNHPAIMNKAWSEIRECVGESRLVEEGDVSS 371
            IIKG++L ++ AGTDT+  TLEWA+S +LNHP ++NKA  EI   +G  RL++E D+S+
Sbjct: 301 RIIKGNMLALILAGTDTSAVTLEWALSNVLNHPDVLNKARDEIDRKIGLDRLMDESDISN 360

Query: 372 LKYLVAIIYETLRLFPAAPLLVPHESSEECRVEGFDIPKGTMLLVNAWAIHRDPRVWEDP 431
           L YL  I+ ETLRL+PAAP+L+PH +SE+C+V G+D+P+GT+LL N WAIHRDP++W+DP
Sbjct: 361 LPYLQNIVSETLRLYPAAPMLLPHVASEDCKVAGYDMPRGTILLTNVWAIHRDPQLWDDP 420

Query: 432 TSFRPERFLNWETAESHKWVPFGVGRRACPGAALAHRVVGLTLATLIQCFEWQRVGEEPI 491
            SF+PERF   +  E+ K +PFG+GRRACPG+ LAHR++ LTL +LIQC EW+++GEE +
Sbjct: 421 MSFKPERF--EKEGEAQKLMPFGLGRRACPGSGLAHRLINLTLGSLIQCLEWEKIGEE-V 480

Query: 492 DLSQGTGITTPKAIPLEAMCRARSSMLHLLS 518
           D+S+G G+T PKA PLEAMCRAR S++ + +
Sbjct: 481 DMSEGKGVTMPKAKPLEAMCRARPSVVKIFN 494

[Close]
BLAST of CmaCh09G002670 vs. TAIR 10
Match: AT4G37330.1 (cytochrome P450, family 81, subfamily D, polypeptide 4 )

HSP 1 Score: 489.2 bits (1258), Expect = 4.1e-138
Identity = 256/503 (50.89%), Postives = 339/503 (67.40%), Query Frame = 0 

Query: 12  MEANTITCLSLSLLLTLILIYKLRKQSPRNRPPSPP-SLPLIGHLHLLKQPFHRVLQDLS 71
           M+ N I  LS   L TL +    R +   N PPSP  SLP+IGHLHLLK P HR    LS
Sbjct: 1   MDLNQILILSFLSLFTLAIFLLTRSKRKLNLPPSPAISLPVIGHLHLLKPPLHRTFLSLS 60

Query: 72  NKHG--PILSLSIGSRPLVVISSPTAVRECFTKNDIALANRPRLLAGKYMNYNYTGVAFA 131
              G  P+  L +G+R + VISS +   ECFTKND+ LANRP+    K++ YN T +  A
Sbjct: 61  KSIGNAPVFHLRLGNRLVYVISSRSIAEECFTKNDVVLANRPKFTISKHLGYNATYLLSA 120

Query: 132 SYGHHWRNMRRIATTELLSNHRLNAYLNIRVQEL-KLVKNLYGATGESSDFVRLEMQSKL 191
           SYG HWRN+RRIA  E+ S HRLN++L IR  E+ +L+ +L  +      FV +EM++ L
Sbjct: 121 SYGDHWRNLRRIAAVEIFSTHRLNSFLYIRKDEIRRLISHL--SRDSLHGFVEVEMKTLL 180

Query: 192 NELSFNIVMRMVSGKRYFGVDVEDVEEAREFREIMKELLQLGGASNPADFLPILRLFDYQ 251
             L+ N  +RM++GKRYFG   ED ++A+  + ++ E +   GA NP D+L ILR     
Sbjct: 181 TNLASNTTIRMLAGKRYFG---EDNDDAKLVKNLVSEAVTSAGAGNPIDYLSILRW--VS 240

Query: 252 GLEKRMVQASGRADSFLQNLIDCERDRRASRWPEEKRGNKTMIQSLLSLQESQPQHYSDD 311
             EKR+     R D+FLQ L+D   ++RA     EK   +TMI  LL+LQ+ QP +Y+D 
Sbjct: 241 SYEKRIKNLGNRFDTFLQKLVD---EKRA-----EKEKGETMIDHLLALQDIQPDYYTDV 300

Query: 312 IIKGHVLTMLAAGTDTTTGTLEWAMSLLLNHPAIMNKAWSEIRECVGESRLVEEGDVSSL 371
           IIKG +LT++ AGTDT++ TLEWAMS LLNHP I+ KA  EI E VG  RLV+E D+ +L
Sbjct: 301 IIKGIILTLIIAGTDTSSVTLEWAMSNLLNHPEILKKARMEIDEKVGLDRLVDESDIVNL 360

Query: 372 KYLVAIIYETLRLFPAAPLLVPHESSEECRVEGFDIPKGTMLLVNAWAIHRDPRVWEDPT 431
            YL +I+ ETLR++PA PLL+PH SSE+C+V G+DIP GTM+L NAWA+HRDP VWEDP 
Sbjct: 361 SYLQSIVLETLRMYPAVPLLLPHLSSEDCKVGGYDIPSGTMVLTNAWAMHRDPEVWEDPE 420

Query: 432 SFRPERFLNWETAESHKWVPFGVGRRACPGAALAHRVVGLTLATLIQCFEWQRVGEEPID 491
            F+PERF   +  E+ K + FG+GRRACPGA LAHR++   L +L+QCFEW+RVGE+ +D
Sbjct: 421 IFKPERF--EKEGEAEKLISFGMGRRACPGAGLAHRLINQALGSLVQCFEWERVGEDFVD 480

Query: 492 LSQGTGITTPKAIPLEAMCRARS 511
           +++  G T PKAIPL AMC+ARS
Sbjct: 481 MTEDKGATLPKAIPLRAMCKARS 486

[Close]
BLAST of CmaCh09G002670 vs. TAIR 10
Match: AT4G37340.1 (cytochrome P450, family 81, subfamily D, polypeptide 3 )

HSP 1 Score: 480.7 bits (1236), Expect = 1.5e-135
Identity = 255/503 (50.70%), Postives = 349/503 (69.38%), Query Frame = 0 

Query: 12  MEANTITCLSLSLLLTLILIYKLRKQSPRNRPPSPP-SLPLIGHLHLLKQPFHRVLQDLS 71
           ME  T+    L + L+L  I    K+ P N PPSP  +LP+IGHL LLK P HRV   +S
Sbjct: 1   MEGQTLIFTFLFISLSLTFIIGRIKRRP-NLPPSPSWALPVIGHLRLLKPPLHRVFLSVS 60

Query: 72  NKHG--PILSLSIGSRPLVVISSPTAVRECFTKNDIALANRPRLLAGKYMNYNYTGVAFA 131
              G  PI+SL +G+R + V+SS +   ECFTKND+ LANR   LA K+++Y  T V  A
Sbjct: 61  ESLGDAPIISLRLGNRLVFVVSSHSLAEECFTKNDVVLANRFNSLASKHISYGCTTVVTA 120

Query: 132 SYGHHWRNMRRIATTELLSNHRLNAYLNIRVQEL-KLVKNLYGATGESSDFVRLEMQSKL 191
           SYG HWRN+RRI   E+ S HRLN++ +IR  E+ +L+  L  +   S +F ++EM+S  
Sbjct: 121 SYGDHWRNLRRIGAVEIFSAHRLNSFSSIRRDEIHRLIACL--SRNSSLEFTKVEMKSMF 180

Query: 192 NELSFNIVMRMVSGKRYFGVDVEDVEEAREFREIMKELLQLGGASNPADFLPILRLFDYQ 251
           + L+FN ++RM++GK Y+G   ED  EA+  RE++ E +   GA N AD+LPIL      
Sbjct: 181 SNLTFNNIIRMLAGKCYYGDGAEDDPEAKRVRELIAEGMGCFGAGNTADYLPILTWI--T 240

Query: 252 GLEKRMVQASGRADSFLQNLIDCERDRRASRWPEEKRGNKTMIQSLLSLQESQPQHYSDD 311
           G EKR+ + + R D FLQ L+D  R+ +      EKR N TM+  LL LQE+QP++Y+D+
Sbjct: 241 GSEKRIKKIASRLDEFLQGLVDERREGK------EKRQN-TMVDHLLCLQETQPEYYTDN 300

Query: 312 IIKGHVLTMLAAGTDTTTGTLEWAMSLLLNHPAIMNKAWSEIRECVGESRLVEEGDVSSL 371
           IIKG +L+++ AGTDT+  TLEW +S LLNHP I++KA  EI   VG +RLVEE D+S L
Sbjct: 301 IIKGIMLSLILAGTDTSAVTLEWTLSALLNHPQILSKARDEIDNKVGLNRLVEESDLSHL 360

Query: 372 KYLVAIIYETLRLFPAAPLLVPHESSEECRVEGFDIPKGTMLLVNAWAIHRDPRVWEDPT 431
            YL  I+ E+LRL+PA+PLLVPH +SE+C+V G+ +P+GTMLL NAWAIHRDP++W+DPT
Sbjct: 361 PYLQNIVSESLRLYPASPLLVPHVASEDCKVGGYHMPRGTMLLTNAWAIHRDPKIWDDPT 420

Query: 432 SFRPERFLNWETAESHKWVPFGVGRRACPGAALAHRVVGLTLATLIQCFEWQRVGEEPID 491
           SF+PERF   +  E+ K + FG+GRRACPG+ LA R+  LT+ +LIQCFEW+R+GEE +D
Sbjct: 421 SFKPERF--EKEGEAQKLLGFGLGRRACPGSGLAQRLASLTIGSLIQCFEWERIGEEEVD 480

Query: 492 LSQ-GTGITTPKAIPLEAMCRAR 510
           +++ G G+  PKAIPL AMC+AR
Sbjct: 481 MTEGGGGVIMPKAIPLVAMCKAR 489

[Close]
BLAST of CmaCh09G002670 vs. TAIR 10
Match: AT4G37360.1 (cytochrome P450, family 81, subfamily D, polypeptide 2 )

HSP 1 Score: 478.8 bits (1231), Expect = 5.6e-135
Identity = 249/503 (49.50%), Postives = 351/503 (69.78%), Query Frame = 0 

Query: 12  MEANTITCLSLSLLLTLI-LIYKLRKQSPRNRPPSPP-SLPLIGHLHLLKQPFHRVLQDL 71
           MEA  +      ++L+LI LI +++++   N PPSP  +LP+IGHL LLK P HRV   +
Sbjct: 1   MEALMLIFTFCFIVLSLIFLIGRIKRK--LNLPPSPAWALPVIGHLRLLKPPLHRVFLSV 60

Query: 72  SNKHG--PILSLSIGSRPLVVISSPTAVRECFTKNDIALANRPRLLAGKYMNYNYTGVAF 131
           S   G  PI+SL +G+R L V+SS +   ECFTKND+ LANR   ++ K+++Y  + V  
Sbjct: 61  SQSLGDAPIISLRLGNRLLFVVSSHSIAEECFTKNDVILANRQTTISTKHISYGNSTVVS 120

Query: 132 ASYGHHWRNMRRIATTELLSNHRLNAYLNIRVQEL-KLVKNLYGATGESSDFVRLEMQSK 191
           ASY  HWRN+RRI   E+ S HRLN++ +IR  E+ +L+  L      S  F ++EM+S 
Sbjct: 121 ASYSEHWRNLRRIGALEIFSAHRLNSFSSIRRDEIRRLIGRL--LRNSSYGFTKVEMKSM 180

Query: 192 LNELSFNIVMRMVSGKRYFGVDVEDVEEAREFREIMKELLQLGGASNPADFLPILRLFDY 251
            ++L+FN ++RM++GK Y+G   ED  EA+  R ++ E +   G  N AD++PIL    Y
Sbjct: 181 FSDLTFNNIIRMLAGKCYYGDGKEDDPEAKRVRTLIAEAMSSSGPGNAADYIPILTWITY 240

Query: 252 QGLEKRMVQASGRADSFLQNLIDCERDRRASRWPEEKRGNKTMIQSLLSLQESQPQHYSD 311
              E R+ + +GR D FLQ L+D +R+ +      EK+ N TM+  LL LQE+QP++Y D
Sbjct: 241 S--ETRIKKLAGRLDEFLQGLVDEKREGK------EKKEN-TMVDHLLCLQETQPEYYMD 300

Query: 312 DIIKGHVLTMLAAGTDTTTGTLEWAMSLLLNHPAIMNKAWSEIRECVGESRLVEEGDVSS 371
            IIKG +L+++A GTDTT  TLEWA+S LLN+P ++NKA  EI   +G  RL+EE D+ +
Sbjct: 301 RIIKGTMLSLIAGGTDTTAVTLEWALSSLLNNPEVLNKARDEIDRMIGVDRLLEESDIPN 360

Query: 372 LKYLVAIIYETLRLFPAAPLLVPHESSEECRVEGFDIPKGTMLLVNAWAIHRDPRVWEDP 431
           L YL  I+ ETLRL+PAAP+L+PH +S++C+V G+D+P+GTMLL NAWAIHRDP +W+DP
Sbjct: 361 LPYLQNIVSETLRLYPAAPMLLPHVASKDCKVGGYDMPRGTMLLTNAWAIHRDPLLWDDP 420

Query: 432 TSFRPERFLNWETAESHKWVPFGVGRRACPGAALAHRVVGLTLATLIQCFEWQRVGEEPI 491
           TSF+PERF   +  E+ K +PFG+GRRACPG+ LA R+V L+L +LIQCFEW+R+GEE +
Sbjct: 421 TSFKPERF--EKEGEAKKLMPFGLGRRACPGSGLAQRLVTLSLGSLIQCFEWERIGEEEV 480

Query: 492 DLSQGTGITTPKAIPLEAMCRAR 510
           D+++G G+T PKA PLEAMCRAR
Sbjct: 481 DMTEGPGLTMPKARPLEAMCRAR 488

[Close]
BLAST of CmaCh09G002670 vs. TAIR 10
Match: AT5G36220.1 (cytochrome p450 81d1 )

HSP 1 Score: 471.5 bits (1212), Expect = 8.9e-133
Identity = 244/509 (47.94%), Postives = 336/509 (66.01%), Query Frame = 0 

Query: 17  ITCLSLSLLLTLILIYKLRKQSPRNRPPSPPS-LPLIGHLHLLKQPFHRVLQDLS----- 76
           +   S+  L+ LI+ +K  K   +N PPSPP  LP+IGHL LLK P HR L+  S     
Sbjct: 8   VVLYSIFSLIFLIISFKFLKPKKQNLPPSPPGWLPIIGHLRLLKPPIHRTLRSFSETLDH 67

Query: 77  NKHGPILSLSIGSRPLVVISS-PTAVRECFTKNDIALANRPRLLAGKYMNYNYTGVAFAS 136
           N  G ++SL +GSR + V+SS   A  ECF KND+ LANRP+++ GK++ YN T +  A 
Sbjct: 68  NDGGGVMSLRLGSRLVYVVSSHKVAAEECFGKNDVVLANRPQVIIGKHVGYNNTNMIAAP 127

Query: 137 YGHHWRNMRRIATTELLSNHRLNAYLNIRVQEL-KLVKNLYGATGESSDFVRLEMQSKLN 196
           YG HWRN+RR+ T E+ S HRLN +L +R  E+ +L+  L    G     V  E++  L 
Sbjct: 128 YGDHWRNLRRLCTIEIFSTHRLNCFLYVRTDEVRRLISRLSRLAGTKKTVV--ELKPMLM 187

Query: 197 ELSFNIVMRMVSGKRYFGVDVEDVEEAREFREIMKELLQLGGASNPADFLPILRLFDYQG 256
           +L+FN +MRM++GKRY+G +  D EEA+  R+++ ++     + N  D++PILRLF    
Sbjct: 188 DLTFNNIMRMMTGKRYYGEETTDEEEAKRVRKLVADVGANTSSGNAVDYVPILRLF--SS 247

Query: 257 LEKRMVQASGRADSFLQNLIDCERDRRASRWPEEKRGNKTMIQSLLSLQESQPQHYSDDI 316
            E R+ +     D FLQ LID +R ++ +          TMI  LL LQ+S  ++Y+D I
Sbjct: 248 YENRVKKLGEETDKFLQGLIDDKRGQQET--------GTTMIDHLLVLQKSDIEYYTDQI 307

Query: 317 IKGHVLTMLAAGTDTTTGTLEWAMSLLLNHPAIMNKAWSEIRECVGESRLVEEGDVSSLK 376
           IKG +L M+ AGT+T+  TLEWA+S LLNHP +++KA  EI   VG  RL+EE D+S L 
Sbjct: 308 IKGIILIMVIAGTNTSAVTLEWALSNLLNHPDVISKARDEIDNRVGLDRLIEEADLSELP 367

Query: 377 YLVAIIYETLRLFPAAPLLVPHESSEECRVEGFDIPKGTMLLVNAWAIHRDPRVWEDPTS 436
           YL  I+ ETLRL PA PLLVPH +SE+C++  +D+P+GT LLVNAWAIHRDP  W+DP S
Sbjct: 368 YLKNIVLETLRLHPATPLLVPHMASEDCKIGSYDMPRGTTLLVNAWAIHRDPNTWDDPDS 427

Query: 437 FRPERFLNWETAESHKWVPFGVGRRACPGAALAHRVVGLTLATLIQCFEWQRVGEEPIDL 496
           F+PERF   E  E+ K + FG+GRRACPG+ LA R+VGL L +LIQCFEW+RVG   +D+
Sbjct: 428 FKPERFEKEE--EAQKLLAFGLGRRACPGSGLAQRIVGLALGSLIQCFEWERVGNVEVDM 487

Query: 497 SQGTGITTPKAIPLEAMCRARSSMLHLLS 518
            +G G T PKAIPL+A+C+AR  +  ++S
Sbjct: 488 KEGVGNTVPKAIPLKAICKARPFLHKIIS 502

The following BLAST results are available for this feature:
BLAST of CmaCh09G002670 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
W8JMU77.8e-15053.14Cytochrome P450 81Q32 OS=Catharanthus roseus OX=4058 GN=CYP81Q32 PE=2 SV=1[more]
Q6WNQ93.2e-14351.41Isoflavone 3'-hydroxylase (Fragment) OS=Medicago truncatula OX=3880 GN=CYP81E9 P... [more]
Q6WNQ81.2e-14253.14Cytochrome P450 81E8 OS=Medicago truncatula OX=3880 GN=CYP81E8 PE=2 SV=1[more]
P931471.6e-13949.10Isoflavone 2'-hydroxylase OS=Glycyrrhiza echinata OX=46348 GN=CYP81E1 PE=1 SV=2[more]
Q6WNR05.4e-13547.66Isoflavone 2'-hydroxylase OS=Medicago truncatula OX=3880 GN=CYP81E7 PE=1 SV=1[more]
BLAST of CmaCh09G002670 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A6J1IL263.5e-302100.00isoflavone 3'-hydroxylase-like OS=Cucurbita maxima OX=3661 GN=LOC111477203 PE=3 ... [more]
A0A6J1F8L92.8e-27593.12isoflavone 3'-hydroxylase-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC11... [more]
A0A5A7VB121.4e-22374.95Cytochrome P450 81E8-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffo... [more]
A0A1S3BWG93.9e-22174.57LOW QUALITY PROTEIN: cytochrome P450 81E8-like OS=Cucumis melo OX=3656 GN=LOC103... [more]
A0A6J1C9L71.2e-18262.43isoflavone 2'-hydroxylase-like OS=Momordica charantia OX=3673 GN=LOC111009467 PE... [more]
BLAST of CmaCh09G002670 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_022977005.17.2e-302100.00isoflavone 3'-hydroxylase-like [Cucurbita maxima][more]
XP_023535658.13.7e-28293.46isoflavone 2'-hydroxylase-like [Cucurbita pepo subsp. pepo][more]
XP_022936846.15.7e-27593.12isoflavone 3'-hydroxylase-like isoform X1 [Cucurbita moschata] >XP_022936847.1 i... [more]
KAG7024282.11.3e-27192.34Cytochrome P450 81E8, partial [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_038896686.14.9e-22676.22cytochrome P450 81Q32-like [Benincasa hispida][more]
BLAST of CmaCh09G002670 vs. TAIR 10
Match NameE-valueIdentityDescription
AT4G37370.15.0e-14450.68cytochrome P450, family 81, subfamily D, polypeptide 8 [more]
AT4G37330.14.1e-13850.89cytochrome P450, family 81, subfamily D, polypeptide 4 [more]
AT4G37340.11.5e-13550.70cytochrome P450, family 81, subfamily D, polypeptide 3 [more]
AT4G37360.15.6e-13549.50cytochrome P450, family 81, subfamily D, polypeptide 2 [more]
AT5G36220.18.9e-13347.94cytochrome p450 81d1 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002401Cytochrome P450, E-class, group IPRINTSPR00463EP450Icoord: 455..478
score: 36.42
coord: 308..325
score: 31.96
coord: 94..115
score: 23.01
coord: 328..354
score: 34.63
coord: 371..389
score: 39.5
coord: 70..89
score: 37.11
coord: 190..208
score: 24.05
coord: 445..455
score: 52.74
coord: 412..436
score: 42.84
IPR001128Cytochrome P450PRINTSPR00385P450coord: 372..383
score: 44.41
coord: 455..466
score: 32.62
coord: 446..455
score: 57.25
coord: 319..336
score: 43.24
IPR001128Cytochrome P450PFAMPF00067p450coord: 43..497
e-value: 1.1E-96
score: 324.4
IPR036396Cytochrome P450 superfamilyGENE3D1.10.630.10Cytochrome P450coord: 32..514
e-value: 8.4E-125
score: 419.0
IPR036396Cytochrome P450 superfamilySUPERFAMILY48264Cytochrome P450coord: 43..509
NoneNo IPR availablePIRSRPIRSR000047-1PIRSR000047-1coord: 12..483
e-value: 6.7E-20
score: 69.3
NoneNo IPR availablePANTHERPTHR47947:SF27SUBFAMILY NOT NAMEDcoord: 36..514
NoneNo IPR availablePANTHERPTHR47947CYTOCHROME P450 82C3-RELATEDcoord: 36..514
IPR017972Cytochrome P450, conserved sitePROSITEPS00086CYTOCHROME_P450coord: 448..457

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh09G002670.1CmaCh09G002670.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0020037 heme binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0016709 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of one atom of oxygen
molecular_function GO:0004497 monooxygenase activity
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen