CmaCh09G001830 (gene) Cucurbita maxima (Rimu) v1.1

Overview


Name	CmaCh09G001830
Type	gene
Organism	Cucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Description	cytochrome P450 CYP82D47-like
Location	Cma_Chr09: 770192 .. 771820 (-)
RNA-Seq Expression	CmaCh09G001830
Synteny	CmaCh09G001830

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAAATTACTCACATCTTACCTGCAATCTTCGCCCTTCTCCTCCTTCTCTATGCTCTCTTTCGTATAGCTTCTCCGAGATCCGCCGCTCACCTCACCAACAAGACACTTCCACCTGAAGCCGCCGGTCGTTTGCCACTGATCGGCCACCTCCATAAACTAAGTGCAACAGAGCCAACTCACATAACATTAGCCAAAATGGCCGACGCCTACGGACCTATTTTTACCTTAAGATTGGGCATGAACACAGCCCTTGTCGTGAGCAGTTGGGAAATAGCGCGAGAGTGTTTCACCACAAACGACAGAATTTTCGCTTCCCGCTCAAAAGTTGTTGCCTCAGAGCTGCTCGGCTACAACTACGCAGCCTTGGGAATTAGCCCCTACGGTCCATACTGGCGCAAAGTGCGCAAACTAGCCACGCTTGAACTGTTGACGAACCATCGCCTCGAGCAGCTCCAACACATTAGAATATGTGAGGTTCAAAATTCGATCAAGAAATTGTATGAGTTATGTGCCGACAGCAGAAATAAAGGGTCCGGTGGGAAAGTGAGGGTTGAGATGAGGACGTGGTTTGGGGATGTAAGTTTGAATACAATATTTAGGATGGTGGTCGGAAACCGATTCAGCGCTGTTTTCGAAGGCAGTGGTGCGGAGCAGTACAGAGAGGCGTTGAGGGCTTTTTTTGAACTGTTTGGGGCATTTGTTCCTTCAGATTCGTTTTCGTGCCTAAGTTGGTTGGATTTGGGAGGGCATAAGAAGGCCATGAATAAGACGGCTAAGGTGCTTGATGAGATGCTTAGTAAATGGATCAAAGAGCATCGAGAGAAGAAGAAGAATTGGGGTGAAGGTGGAGTGGAAACAGAGGAGCAAGACTTCATGGATGCCATGCTTTCTGCTGTCGACGACGACGGAGGGTTATGTGGTTTTGATGCTGATACAATCACCAAAGCTACTTGTTTGGTAAGCCTATGTATTCAAAACCCATTCCTTACATGAACCAACTCTTACTAAATAACAAATTAAATTATGATATTGTGTAGTCGATGATGTTGGGTGGATACACTACTATGATTGCTACGACATGGGCTCTTTCCCTACTCCTCAACAATCAAGAAACACTTAAGAAGGCCCAACTTGAATTAGATGAAAAAGTTGGAACATCAAGACAAGTAAAAGAGGCCGACTTAAAAAATCTGATATATTTCCAAGCCATTGTGAAGGAGACATTGCGTTTATATCCTGCCGCGCCGCTTTTAATCCCTCATGAATCTATAGAAGATTGTATGGTTTCAGGCTATCATATTCCTGCAAGGACACGCCTTTTAGTGAATGTTCAAAAACTGCAAAAAGACCCACTTGTTTGGGAGGATCCGTGTGAATTTCGTCCAGAAAGATTCTTAACCAACGACACAAAGTTTGATATGCAAGGACAAAATATGCAACTAATGCCATTTGGAAAGGGTAGGCGGATGTGTCCTGGAATGTCATTTGCCCTTCAACTTGTGCATCTTACTCTTGCAAGTCTACTTCATGAATTTGAAATTGATAGACTATCTGAAGAATTATTGGACATGGAAGAGGGTTTTGGATTTATTATTTTTAGAAAAACTCCGCTTGAAATTGTTTTAA

mRNA sequence

ATGGAAATTACTCACATCTTACCTGCAATCTTCGCCCTTCTCCTCCTTCTCTATGCTCTCTTTCGTATAGCTTCTCCGAGATCCGCCGCTCACCTCACCAACAAGACACTTCCACCTGAAGCCGCCGGTCGTTTGCCACTGATCGGCCACCTCCATAAACTAAGTGCAACAGAGCCAACTCACATAACATTAGCCAAAATGGCCGACGCCTACGGACCTATTTTTACCTTAAGATTGGGCATGAACACAGCCCTTGTCGTGAGCAGTTGGGAAATAGCGCGAGAGTGTTTCACCACAAACGACAGAATTTTCGCTTCCCGCTCAAAAGTTGTTGCCTCAGAGCTGCTCGGCTACAACTACGCAGCCTTGGGAATTAGCCCCTACGGTCCATACTGGCGCAAAGTGCGCAAACTAGCCACGCTTGAACTGTTGACGAACCATCGCCTCGAGCAGCTCCAACACATTAGAATATGTGAGGTTCAAAATTCGATCAAGAAATTGTATGAGTTATGTGCCGACAGCAGAAATAAAGGGTCCGGTGGGAAAGTGAGGGTTGAGATGAGGACGTGGTTTGGGGATGTAAGTTTGAATACAATATTTAGGATGGTGGTCGGAAACCGATTCAGCGCTGTTTTCGAAGGCAGTGGTGCGGAGCAGTACAGAGAGGCGTTGAGGGCTTTTTTTGAACTGTTTGGGGCATTTGTTCCTTCAGATTCGTTTTCGTGCCTAAGTTGGTTGGATTTGGGAGGGCATAAGAAGGCCATGAATAAGACGGCTAAGGTGCTTGATGAGATGCTTAGTAAATGGATCAAAGAGCATCGAGAGAAGAAGAAGAATTGGGGTGAAGGTGGAGTGGAAACAGAGGAGCAAGACTTCATGGATGCCATGCTTTCTGCTGTCGACGACGACGGAGGGTTATGTGGTTTTGATGCTGATACAATCACCAAAGCTACTTGTTTGTCGATGATGTTGGGTGGATACACTACTATGATTGCTACGACATGGGCTCTTTCCCTACTCCTCAACAATCAAGAAACACTTAAGAAGGCCCAACTTGAATTAGATGAAAAAGTTGGAACATCAAGACAAGTAAAAGAGGCCGACTTAAAAAATCTGATATATTTCCAAGCCATTGTGAAGGAGACATTGCGTTTATATCCTGCCGCGCCGCTTTTAATCCCTCATGAATCTATAGAAGATTGTATGGTTTCAGGCTATCATATTCCTGCAAGGACACGCCTTTTAGTGAATGTTCAAAAACTGCAAAAAGACCCACTTGTTTGGGAGGATCCGTGTGAATTTCGTCCAGAAAGATTCTTAACCAACGACACAAAGTTTGATATGCAAGGACAAAATATGCAACTAATGCCATTTGGAAAGGGTAGGCGGATGTGTCCTGGAATGTCATTTGCCCTTCAACTTGTGCATCTTACTCTTGCAAGTCTACTTCATGAATTTGAAATTGATAGACTATCTGAAGAATTATTGGACATGGAAGAGGAAAAACTCCGCTTGAAATTGTTTTAA

Coding sequence (CDS)

ATGGAAATTACTCACATCTTACCTGCAATCTTCGCCCTTCTCCTCCTTCTCTATGCTCTCTTTCGTATAGCTTCTCCGAGATCCGCCGCTCACCTCACCAACAAGACACTTCCACCTGAAGCCGCCGGTCGTTTGCCACTGATCGGCCACCTCCATAAACTAAGTGCAACAGAGCCAACTCACATAACATTAGCCAAAATGGCCGACGCCTACGGACCTATTTTTACCTTAAGATTGGGCATGAACACAGCCCTTGTCGTGAGCAGTTGGGAAATAGCGCGAGAGTGTTTCACCACAAACGACAGAATTTTCGCTTCCCGCTCAAAAGTTGTTGCCTCAGAGCTGCTCGGCTACAACTACGCAGCCTTGGGAATTAGCCCCTACGGTCCATACTGGCGCAAAGTGCGCAAACTAGCCACGCTTGAACTGTTGACGAACCATCGCCTCGAGCAGCTCCAACACATTAGAATATGTGAGGTTCAAAATTCGATCAAGAAATTGTATGAGTTATGTGCCGACAGCAGAAATAAAGGGTCCGGTGGGAAAGTGAGGGTTGAGATGAGGACGTGGTTTGGGGATGTAAGTTTGAATACAATATTTAGGATGGTGGTCGGAAACCGATTCAGCGCTGTTTTCGAAGGCAGTGGTGCGGAGCAGTACAGAGAGGCGTTGAGGGCTTTTTTTGAACTGTTTGGGGCATTTGTTCCTTCAGATTCGTTTTCGTGCCTAAGTTGGTTGGATTTGGGAGGGCATAAGAAGGCCATGAATAAGACGGCTAAGGTGCTTGATGAGATGCTTAGTAAATGGATCAAAGAGCATCGAGAGAAGAAGAAGAATTGGGGTGAAGGTGGAGTGGAAACAGAGGAGCAAGACTTCATGGATGCCATGCTTTCTGCTGTCGACGACGACGGAGGGTTATGTGGTTTTGATGCTGATACAATCACCAAAGCTACTTGTTTGTCGATGATGTTGGGTGGATACACTACTATGATTGCTACGACATGGGCTCTTTCCCTACTCCTCAACAATCAAGAAACACTTAAGAAGGCCCAACTTGAATTAGATGAAAAAGTTGGAACATCAAGACAAGTAAAAGAGGCCGACTTAAAAAATCTGATATATTTCCAAGCCATTGTGAAGGAGACATTGCGTTTATATCCTGCCGCGCCGCTTTTAATCCCTCATGAATCTATAGAAGATTGTATGGTTTCAGGCTATCATATTCCTGCAAGGACACGCCTTTTAGTGAATGTTCAAAAACTGCAAAAAGACCCACTTGTTTGGGAGGATCCGTGTGAATTTCGTCCAGAAAGATTCTTAACCAACGACACAAAGTTTGATATGCAAGGACAAAATATGCAACTAATGCCATTTGGAAAGGGTAGGCGGATGTGTCCTGGAATGTCATTTGCCCTTCAACTTGTGCATCTTACTCTTGCAAGTCTACTTCATGAATTTGAAATTGATAGACTATCTGAAGAATTATTGGACATGGAAGAGGAAAAACTCCGCTTGAAATTGTTTTAA

Protein sequence

MEITHILPAIFALLLLLYALFRIASPRSAAHLTNKTLPPEAAGRLPLIGHLHKLSATEPTHITLAKMADAYGPIFTLRLGMNTALVVSSWEIARECFTTNDRIFASRSKVVASELLGYNYAALGISPYGPYWRKVRKLATLELLTNHRLEQLQHIRICEVQNSIKKLYELCADSRNKGSGGKVRVEMRTWFGDVSLNTIFRMVVGNRFSAVFEGSGAEQYREALRAFFELFGAFVPSDSFSCLSWLDLGGHKKAMNKTAKVLDEMLSKWIKEHREKKKNWGEGGVETEEQDFMDAMLSAVDDDGGLCGFDADTITKATCLSMMLGGYTTMIATTWALSLLLNNQETLKKAQLELDEKVGTSRQVKEADLKNLIYFQAIVKETLRLYPAAPLLIPHESIEDCMVSGYHIPARTRLLVNVQKLQKDPLVWEDPCEFRPERFLTNDTKFDMQGQNMQLMPFGKGRRMCPGMSFALQLVHLTLASLLHEFEIDRLSEELLDMEEEKLRLKLF

Homology

[Close]

BLAST of CmaCh09G001830 vs. ExPASy Swiss-Prot
Match: O49858 (Cytochrome P450 82A3 OS=Glycine max OX=3847 GN=CYP82A3 PE=2 SV=1)

HSP 1 Score: 441.8 bits (1135), Expect = 1.0e-122
Identity = 232/501 (46.31%), Postives = 330/501 (65.87%), Query Frame = 0


Query: 4   THILPAIFALLLLLYALFRIASPRSAAHLTNKTLPPEAAGRLPLIGHLHKLSATEPTHIT 63
           T  + +I +L+ L   L+R  S    A        P  +G  P++GHL  L+ ++  H  
Sbjct: 12  TIAIASILSLIFLCLFLYRKNSRGKDA--------PVVSGAWPILGHLSLLNGSQTPHKV 71

Query: 64  LAKMADAYGPIFTLRLGMNTALVVSSWEIARECFTTNDRIFASRSKVVASELLGYNYAAL 123
           L  +AD YGP+FT++LGM  ALV+S+WE+++E FTTND   +SR K+VA E++ YN A +
Sbjct: 72  LGALADKYGPLFTIKLGMKPALVLSNWEMSKELFTTNDLAVSSRPKLVAVEVMSYNQAFV 131

Query: 124 GISPYGPYWRKVRKLATLELLTNHRLEQLQHIRICEVQNSIKKLYELCADSRNKGSGGKV 183
           G++PYGPYWR++RK+ T E L+N R+EQ  HIR+ EV+ SIK+L+++ ++  NK      
Sbjct: 132 GLAPYGPYWRELRKIVTFEFLSNRRIEQRNHIRVSEVRTSIKELFDIWSNG-NKNESRYT 191

Query: 184 RVEMRTWFGDVSLNTIFRMVVGNRFSAVFEGSG---AEQYREALRAFFELFGAFVPSDSF 243
            V+++ W   ++ N + RMVVG R+  V    G   A+++ + +R F  L G F  +D  
Sbjct: 192 LVDIKQWLAYLTFNMVVRMVVGKRYFGVMHVEGKDKAQRFMKNIREFMNLMGTFTVADGV 251

Query: 244 SCLSWLDLGGHKKAMNKTAKVLDEMLSKWIKEHREKKKNWGEGGVETEEQDFMDAMLSAV 303
            CL WLDLGGH+KAM   AK +D++LS+W++EHR+KK   GE  VE+ ++DFMD M+SA+
Sbjct: 252 PCLRWLDLGGHEKAMKANAKEVDKLLSEWLEEHRQKKL-LGE-NVES-DRDFMDVMISAL 311

Query: 304 DDDGGLCGFDADTITKATCLSMMLGGY-TTMIATTWALSLLLNNQETLKKAQLELDEKVG 363
            +   +  FDADTI KAT L ++LGG  +T +  TWALSLLL N   L KA+ E+D ++G
Sbjct: 312 -NGAQIGAFDADTICKATSLELILGGTDSTAVTLTWALSLLLRNPLALGKAKEEIDMQIG 371

Query: 364 TSRQVKEADLKNLIYFQAIVKETLRLYPAAPLLIPHESIEDCMVSGYHIPARTRLLVNVQ 423
               ++E+D+  L+Y QAIVKETLRLYP AP   P E  E+C++ GYHI   TRL+ N+ 
Sbjct: 372 KDEYIRESDISKLVYLQAIVKETLRLYPPAPFSSPREFTENCILGGYHIKKGTRLIHNLW 431

Query: 424 KLQKDPLVWEDPCEFRPERFLTNDTKFDMQGQNMQLMPFGKGRRMCPGMSFALQLVHLTL 483
           K+ +DP VW DP EF+PERFLT     D++G N +L+PFG GRR+C GMS  L +VH TL
Sbjct: 432 KIHRDPSVWSDPLEFKPERFLTTHKDVDLRGHNFELLPFGSGRRVCAGMSLGLNMVHFTL 491

Query: 484 ASLLHEFEIDRLSEELLDMEE 501
           A+LLH F+I   S E +DM E
Sbjct: 492 ANLLHSFDILNPSAEPVDMTE 499

[Close]

BLAST of CmaCh09G001830 vs. ExPASy Swiss-Prot
Match: Q9SZ46 (Xanthotoxin 5-hydroxylase CYP82C4 OS=Arabidopsis thaliana OX=3702 GN=CYP82C4 PE=1 SV=1)

HSP 1 Score: 438.7 bits (1127), Expect = 8.8e-122
Identity = 229/476 (48.11%), Postives = 322/476 (67.65%), Query Frame = 0


Query: 39  PEAAGRLPLIGHLHKLSATEP-THITLAKMADAYGPIFTLRLGMNTALVVSSWEIARECF 98
           P  +G  P+IGHLH L   E   + TL KMAD YGP  +L+LG N A VVSS+E+A++CF
Sbjct: 33  PAPSGAWPIIGHLHLLGGKEQLLYRTLGKMADHYGPAMSLQLGSNEAFVVSSFEVAKDCF 92

Query: 99  TTNDRIFASRSKVVASELLGYNYAALGISPYGPYWRKVRKLATLELLTNHRLEQLQHIRI 158
           T ND+  ASR    A++ +GYN+A  G +PY  +WR++RK+AT+ELL+N RL+ L+H+R+
Sbjct: 93  TVNDKALASRPMTAAAKHMGYNFAVFGFAPYSAFWREMRKIATIELLSNRRLQMLKHVRV 152

Query: 159 CEVQNSIKKLYELCADSRNKGSGGKVRVEMRTWFGDVSLNTIFRMVVGNRFSAVFEGSG- 218
            E+   +K LY L       G    V V++++W  D++LN I RMV G R+   F G G 
Sbjct: 153 SEITMGVKDLYSLWF---KNGGTKPVMVDLKSWLEDMTLNMIVRMVAGKRY---FGGGGS 212

Query: 219 --------AEQYREALRAFFELFGAFVPSDSFSCLSWLDLGGHKKAMNKTAKVLDEMLSK 278
                   A Q ++A+  FF L G F  SD+F  LS+ DL GH+K M +T   LD +L +
Sbjct: 213 VSSEDTEEAMQCKKAIAKFFHLIGIFTVSDAFPTLSFFDLQGHEKEMKQTGSELDVILER 272

Query: 279 WIKEHREKKKNWGEGGVETEEQDFMDAMLSAVDDDGGL--CGFDADTITKATCLSMMLGG 338
           WI+ HR+++K     G +  + DF+D M+S + + G L    +DA+T  K+TCL+++LGG
Sbjct: 273 WIENHRQQRK---FSGTKENDSDFIDVMMS-LAEQGKLSHLQYDANTSIKSTCLALILGG 332

Query: 339 YTTMIAT-TWALSLLLNNQETLKKAQLELDEKVGTSRQVKEADLKNLIYFQAIVKETLRL 398
             T  +T TWA+SLLLNN+E LKKAQ E+D  VG  R V+++D++NL+Y QAI+KETLRL
Sbjct: 333 SDTSASTLTWAISLLLNNKEMLKKAQDEIDIHVGRDRNVEDSDIENLVYLQAIIKETLRL 392

Query: 399 YPAAPLLIPHESIEDCMVSGYHIPARTRLLVNVQKLQKDPLVWEDPCEFRPERFLTNDTK 458
           YPA PLL P E++EDC V+GY++P  TRL+VNV K+Q+DP V+ +P EFRPERF+T + K
Sbjct: 393 YPAGPLLGPREAMEDCTVAGYYVPCGTRLIVNVWKIQRDPKVYMEPNEFRPERFITGEAK 452

Query: 459 -FDMQGQNMQLMPFGKGRRMCPGMSFALQLVHLTLASLLHEFEIDRLSEELLDMEE 501
            FD++GQN +LMPFG GRR CPG S A+Q++HL LA  LH F++  + +  +DM E
Sbjct: 453 EFDVRGQNFELMPFGSGRRSCPGSSLAMQVLHLGLARFLHSFDVKTVMDMPVDMSE 498

[Close]

BLAST of CmaCh09G001830 vs. ExPASy Swiss-Prot
Match: Q43068 (Cytochrome P450 82A1 (Fragment) OS=Pisum sativum OX=3888 GN=CYP82A1 PE=2 SV=2)

HSP 1 Score: 434.5 bits (1116), Expect = 1.7e-120
Identity = 238/513 (46.39%), Postives = 318/513 (61.99%), Query Frame = 0


Query: 9   AIFALLLLLYALFRIASPRSAAHLTNKTLPPEAAGRLPLIGHLHKLSATEPTHITLAKMA 68
           A  +L+ LL+ LFR +       +++   PP  +G  PL+GHL  +  T+  H TL  + 
Sbjct: 12  AFISLISLLFFLFRFSK------VSHTKEPPIISGSWPLLGHLPLMRNTQTPHKTLGALV 71

Query: 69  DAYGPIFTLRLGMNTALVVSSWEIARECFTTNDRIFASRSKVVASELLGYNYAALGISPY 128
           D YGPIFT++LG   ALV+S+WE+A+ECFT ND + +SR K VA EL+ YN A +G +PY
Sbjct: 72  DKYGPIFTIKLGATNALVLSNWELAKECFTKNDIVVSSRPKPVAVELMSYNQAFIGWAPY 131

Query: 129 GPYWRKVRKLATLELLTNHRLEQLQHIRICEVQNSIKKL-----------YELCADSRNK 188
           G YWR++RK+ TLE+L+N R+E L HIR+ EVQ SIK+L           Y L  D+++ 
Sbjct: 132 GAYWRQLRKIVTLEILSNRRIELLSHIRVSEVQTSIKELVNVWSNQISSQYGLLDDTKSS 191

Query: 189 GSGGK------VRVEMRTWFGDVSLNTIFRMVVGNRFSA---VFEGSGAEQYREALRAFF 248
            +  +      V VE++ WF  ++LN + RMVVG R      V     A+++ E +R F 
Sbjct: 192 STNDEPSTTDYVSVELKKWFAQLTLNMVLRMVVGKRCFGDVDVENKEEAKRFLENIRDFM 251

Query: 249 ELFGAFVPSDSFSCLSWLDLGGHKKAMNKTAKVLDEMLSKWIKEHREKKKNWGEGGVETE 308
            L G F   D    L WLDLGGH+K M K AK  D ML++W++EHREKK    E  V   
Sbjct: 252 RLIGTFTVGDGVPFLKWLDLGGHEKEMKKCAKKFDVMLNEWLEEHREKKGLGSEDKV-VG 311

Query: 309 EQDFMDAMLSAVDDDGGLCGFDADTITKATCLSMMLGGYTTMIAT-TWALSLLLNNQETL 368
           E+DFMDAML  + D   + GFD DTI KAT L ++LGG  T   T TWA+ LLL +   L
Sbjct: 312 ERDFMDAMLLVLKDK-PIEGFDVDTIIKATTLELILGGSDTTAGTLTWAMCLLLKHPHVL 371

Query: 369 KKAQLELDEKVGTSRQVKEADLKNLIYFQAIVKETLRLYPAAPLLIPHESIEDCMVSGYH 428
           +K + EL+  +G  R V E+D+  L+Y  AI+KETLRLYP AP   P E  EDC + GYH
Sbjct: 372 EKLKEELNTYIGKERCVNESDINKLVYLHAIIKETLRLYPPAPFSSPREFTEDCTIGGYH 431

Query: 429 IPARTRLLVNVQKLQKDPLVWEDPCEFRPERFLTNDTKFDMQGQNMQLMPFGKGRRMCPG 488
           I   TRL+ N+ K+ +DP VW DP EF+PERFL+     D++GQN +L+PFG GRRMC G
Sbjct: 432 IKKGTRLMPNLWKIHRDPSVWPDPLEFKPERFLSTHKDVDVRGQNFELLPFGSGRRMCAG 491

Query: 489 MSFALQLVHLTLASLLHEFEIDRLSEELLDMEE 501
           MS  L +VH  LA+ LH FEI   S E +D+ E
Sbjct: 492 MSLGLHMVHYILANFLHSFEILNPSPESIDVTE 516

[Close]

BLAST of CmaCh09G001830 vs. ExPASy Swiss-Prot
Match: A0A0N7F297 (Demethylepipodophyllotoxin synthase OS=Sinopodophyllum hexandrum OX=93608 GN=CYP82D61 PE=1 SV=1)

HSP 1 Score: 433.0 bits (1112), Expect = 4.8e-120
Identity = 231/469 (49.25%), Postives = 308/469 (65.67%), Query Frame = 0


Query: 38  PPEAAGRLPLIGHLHKLSATEPTHITLAKMADAYGPIFTLRLGMNTALVVSSWEIARECF 97
           PP+ AG  P+IGHLH L+  +  H  L+  AD  GP+F ++LG++ ALVV++ EIA+ECF
Sbjct: 36  PPQPAGAWPIIGHLHLLARGDLPHKILSSFADKNGPVFKIQLGVHQALVVNNSEIAKECF 95

Query: 98  TTNDRIFASRSKVVASELLGYNYAALGISPYGPYWRKVRKLATLELLTNHRLEQLQHIRI 157
           TTNDR F +R   VA++++GYNY  LG++PYGPYWR +RK+  LE L+N RL+ L+H+  
Sbjct: 96  TTNDRFFLNRPSGVAAKIMGYNYVMLGVAPYGPYWRDMRKIIMLEFLSNRRLQSLKHVWH 155

Query: 158 CEVQNSIKKLYELCADSRNKGSGGKVRVEMRTWFGDVSLNTIFRMVVGNRFSAVFEGSGA 217
            E+  S K+LY+L  +++N        V+M+ W  D++LN   +MVVG RF      S  
Sbjct: 156 SEISISSKELYKLW-ETQNIDF---CLVDMKQWLADLTLNMSVKMVVGKRFFGSASASAC 215

Query: 218 EQYR-----EALRAFFELFGAFVPSDSFSCLSWLDLGGHKKAMNKTAKVLDEMLSKWIKE 277
           E+       + LR  F L G+FV SD    L WLDLGGH+K M +T K LD +   W+ E
Sbjct: 216 EETESSNCPKTLRNMFRLMGSFVLSDYLPYLRWLDLGGHEKEMKRTVKELDILFKGWLDE 275

Query: 278 HREKKKNWGEGGVETEEQDFMDAMLSAVDDDGGLCGFDADTITKATCLSMMLGGYTTMIA 337
           H+ K+ +   GG E ++QDFMD MLS +++     G D DTI K  CL+++LGG  T  A
Sbjct: 276 HKRKRLS---GGKEDDDQDFMDVMLSILEE--SKLGNDVDTINKTACLAIILGGADTTWA 335

Query: 338 T-TWALSLLLNNQETLKKAQLELDEKVGTSRQVKEADLKNLIYFQAIVKETLRLYPAAPL 397
           T TWALSLLLNN   LKKAQ ELD  VG  R V E+DL  L Y  AI+KETLRLYP  PL
Sbjct: 336 TLTWALSLLLNNPNALKKAQDELDLHVGRDRNVDESDLVKLTYIDAIIKETLRLYPPGPL 395

Query: 398 LIPHESIEDCMVSGYHIPARTRLLVNVQKLQKDPLVWEDPCEFRPERFLTNDTKFDMQGQ 457
           L P    EDC ++GYH+ A TRL+VN  K+Q+DPLVW  P E++PERFL  D   DM+GQ
Sbjct: 396 LGPRVVTEDCTIAGYHVRAGTRLIVNAWKIQRDPLVWSQPHEYQPERFLERDV--DMKGQ 455

Query: 458 NMQLMPFGKGRRMCPGMSFALQLVHLTLASLLHEFEIDRLSEELLDMEE 501
           + +L+PFG GRR CP +S ALQ++ LTLA +LH FE+   ++  +DM E
Sbjct: 456 HFELIPFGSGRRACPAISLALQVLPLTLAHILHGFELRTPNQNKVDMTE 493

[Close]

BLAST of CmaCh09G001830 vs. ExPASy Swiss-Prot
Match: O49394 (Xanthotoxin 5-hydroxylase CYP82C2 OS=Arabidopsis thaliana OX=3702 GN=CYP82C2 PE=1 SV=2)

HSP 1 Score: 420.6 bits (1080), Expect = 2.5e-116
Identity = 222/472 (47.03%), Postives = 313/472 (66.31%), Query Frame = 0


Query: 39  PEAAGRLPLIGHLHKLSATEP-THITLAKMADAYGPIFTLRLGMNTALVVSSWEIARECF 98
           P  +G  P+IGHLH LS  E   + TL KMAD YGP  +LRLG +   VVSS+E+A++CF
Sbjct: 33  PAPSGAWPIIGHLHLLSGKEQLLYRTLGKMADQYGPAMSLRLGSSETFVVSSFEVAKDCF 92

Query: 99  TTNDRIFASRSKVVASELLGYNYAALGISPYGPYWRKVRKLATLELLTNHRLEQLQHIRI 158
           T ND+  ASR    A++ +GY+ A  G +PY  +WR++RK+ATLELL+N RL+ L+H+R+
Sbjct: 93  TVNDKALASRPITAAAKHMGYDCAVFGFAPYSAFWREMRKIATLELLSNRRLQMLKHVRV 152

Query: 159 CEVQNSIKKLYELCADSRNKGSGGKVRVEMRTWFGDVSLNTIFRMVVGNRF-----SAVF 218
            E+   ++ LY L      KG    V V++++W  D+SLN + RMV G R+      +  
Sbjct: 153 SEISMVMQDLYSLWV---KKGGSEPVMVDLKSWLEDMSLNMMVRMVAGKRYFGGGSLSPE 212

Query: 219 EGSGAEQYREALRAFFELFGAFVPSDSFSCLSWLDLGGHKKAMNKTAKVLDEMLSKWIKE 278
           +   A Q R+ +  FF L G F  SD+F  L W D  GH+K M +T + LD +L +WI+ 
Sbjct: 213 DAEEARQCRKGVANFFHLVGIFTVSDAFPKLGWFDFQGHEKEMKQTGRELDVILERWIEN 272

Query: 279 HREKKKNWGEGGVETEEQDFMDAMLSAVDDDGGLCGFDADTIT--KATCLSMMLGGYTTM 338
           HR+++K     G +  + DF+D MLS + + G       D IT  K+TCL+++LGG  T 
Sbjct: 273 HRQQRK---VSGTKHNDSDFVDVMLS-LAEQGKFSHLQHDAITSIKSTCLALILGGSETS 332

Query: 339 IAT-TWALSLLLNNQETLKKAQLELDEKVGTSRQVKEADLKNLIYFQAIVKETLRLYPAA 398
            +T TWA+SLLLNN++ LKKAQ E+D  VG  R V+++D++NL+Y QAI+KETLRLYPA 
Sbjct: 333 PSTLTWAISLLLNNKDMLKKAQDEIDIHVGRDRNVEDSDIENLVYIQAIIKETLRLYPAG 392

Query: 399 PLLIPHESIEDCMVSGYHIPARTRLLVNVQKLQKDPLVWEDPCEFRPERFLTNDTK-FDM 458
           PLL   E+IEDC V+GY++   TR+LVNV K+Q+DP V+ +P EFRPERF+T + K FD+
Sbjct: 393 PLLGHREAIEDCTVAGYNVRRGTRMLVNVWKIQRDPRVYMEPNEFRPERFITGEAKEFDV 452

Query: 459 QGQNMQLMPFGKGRRMCPGMSFALQLVHLTLASLLHEFEIDRLSEELLDMEE 501
           +GQN +LMPFG GRR CPG S A+Q++HL LA  L  F++  + +  +DM E
Sbjct: 453 RGQNFELMPFGSGRRSCPGSSLAMQVLHLGLARFLQSFDVKTVMDMPVDMTE 497

[Close]

BLAST of CmaCh09G001830 vs. ExPASy TrEMBL
Match: A0A6J1IGR6 (cytochrome P450 CYP82D47-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111476805 PE=3 SV=1)

HSP 1 Score: 993.8 bits (2568), Expect = 2.6e-286
Identity = 500/500 (100.00%), Postives = 500/500 (100.00%), Query Frame = 0


Query: 1   MEITHILPAIFALLLLLYALFRIASPRSAAHLTNKTLPPEAAGRLPLIGHLHKLSATEPT 60
           MEITHILPAIFALLLLLYALFRIASPRSAAHLTNKTLPPEAAGRLPLIGHLHKLSATEPT
Sbjct: 1   MEITHILPAIFALLLLLYALFRIASPRSAAHLTNKTLPPEAAGRLPLIGHLHKLSATEPT 60

Query: 61  HITLAKMADAYGPIFTLRLGMNTALVVSSWEIARECFTTNDRIFASRSKVVASELLGYNY 120
           HITLAKMADAYGPIFTLRLGMNTALVVSSWEIARECFTTNDRIFASRSKVVASELLGYNY
Sbjct: 61  HITLAKMADAYGPIFTLRLGMNTALVVSSWEIARECFTTNDRIFASRSKVVASELLGYNY 120

Query: 121 AALGISPYGPYWRKVRKLATLELLTNHRLEQLQHIRICEVQNSIKKLYELCADSRNKGSG 180
           AALGISPYGPYWRKVRKLATLELLTNHRLEQLQHIRICEVQNSIKKLYELCADSRNKGSG
Sbjct: 121 AALGISPYGPYWRKVRKLATLELLTNHRLEQLQHIRICEVQNSIKKLYELCADSRNKGSG 180

Query: 181 GKVRVEMRTWFGDVSLNTIFRMVVGNRFSAVFEGSGAEQYREALRAFFELFGAFVPSDSF 240
           GKVRVEMRTWFGDVSLNTIFRMVVGNRFSAVFEGSGAEQYREALRAFFELFGAFVPSDSF
Sbjct: 181 GKVRVEMRTWFGDVSLNTIFRMVVGNRFSAVFEGSGAEQYREALRAFFELFGAFVPSDSF 240

Query: 241 SCLSWLDLGGHKKAMNKTAKVLDEMLSKWIKEHREKKKNWGEGGVETEEQDFMDAMLSAV 300
           SCLSWLDLGGHKKAMNKTAKVLDEMLSKWIKEHREKKKNWGEGGVETEEQDFMDAMLSAV
Sbjct: 241 SCLSWLDLGGHKKAMNKTAKVLDEMLSKWIKEHREKKKNWGEGGVETEEQDFMDAMLSAV 300

Query: 301 DDDGGLCGFDADTITKATCLSMMLGGYTTMIATTWALSLLLNNQETLKKAQLELDEKVGT 360
           DDDGGLCGFDADTITKATCLSMMLGGYTTMIATTWALSLLLNNQETLKKAQLELDEKVGT
Sbjct: 301 DDDGGLCGFDADTITKATCLSMMLGGYTTMIATTWALSLLLNNQETLKKAQLELDEKVGT 360

Query: 361 SRQVKEADLKNLIYFQAIVKETLRLYPAAPLLIPHESIEDCMVSGYHIPARTRLLVNVQK 420
           SRQVKEADLKNLIYFQAIVKETLRLYPAAPLLIPHESIEDCMVSGYHIPARTRLLVNVQK
Sbjct: 361 SRQVKEADLKNLIYFQAIVKETLRLYPAAPLLIPHESIEDCMVSGYHIPARTRLLVNVQK 420

Query: 421 LQKDPLVWEDPCEFRPERFLTNDTKFDMQGQNMQLMPFGKGRRMCPGMSFALQLVHLTLA 480
           LQKDPLVWEDPCEFRPERFLTNDTKFDMQGQNMQLMPFGKGRRMCPGMSFALQLVHLTLA
Sbjct: 421 LQKDPLVWEDPCEFRPERFLTNDTKFDMQGQNMQLMPFGKGRRMCPGMSFALQLVHLTLA 480

Query: 481 SLLHEFEIDRLSEELLDMEE 501
           SLLHEFEIDRLSEELLDMEE
Sbjct: 481 SLLHEFEIDRLSEELLDMEE 500

[Close]

BLAST of CmaCh09G001830 vs. ExPASy TrEMBL
Match: A0A6J1IFL2 (cytochrome P450 CYP82D47-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111476805 PE=3 SV=1)

HSP 1 Score: 993.8 bits (2568), Expect = 2.6e-286
Identity = 500/500 (100.00%), Postives = 500/500 (100.00%), Query Frame = 0


Query: 1   MEITHILPAIFALLLLLYALFRIASPRSAAHLTNKTLPPEAAGRLPLIGHLHKLSATEPT 60
           MEITHILPAIFALLLLLYALFRIASPRSAAHLTNKTLPPEAAGRLPLIGHLHKLSATEPT
Sbjct: 1   MEITHILPAIFALLLLLYALFRIASPRSAAHLTNKTLPPEAAGRLPLIGHLHKLSATEPT 60

Query: 61  HITLAKMADAYGPIFTLRLGMNTALVVSSWEIARECFTTNDRIFASRSKVVASELLGYNY 120
           HITLAKMADAYGPIFTLRLGMNTALVVSSWEIARECFTTNDRIFASRSKVVASELLGYNY
Sbjct: 61  HITLAKMADAYGPIFTLRLGMNTALVVSSWEIARECFTTNDRIFASRSKVVASELLGYNY 120

Query: 121 AALGISPYGPYWRKVRKLATLELLTNHRLEQLQHIRICEVQNSIKKLYELCADSRNKGSG 180
           AALGISPYGPYWRKVRKLATLELLTNHRLEQLQHIRICEVQNSIKKLYELCADSRNKGSG
Sbjct: 121 AALGISPYGPYWRKVRKLATLELLTNHRLEQLQHIRICEVQNSIKKLYELCADSRNKGSG 180

Query: 181 GKVRVEMRTWFGDVSLNTIFRMVVGNRFSAVFEGSGAEQYREALRAFFELFGAFVPSDSF 240
           GKVRVEMRTWFGDVSLNTIFRMVVGNRFSAVFEGSGAEQYREALRAFFELFGAFVPSDSF
Sbjct: 181 GKVRVEMRTWFGDVSLNTIFRMVVGNRFSAVFEGSGAEQYREALRAFFELFGAFVPSDSF 240

Query: 241 SCLSWLDLGGHKKAMNKTAKVLDEMLSKWIKEHREKKKNWGEGGVETEEQDFMDAMLSAV 300
           SCLSWLDLGGHKKAMNKTAKVLDEMLSKWIKEHREKKKNWGEGGVETEEQDFMDAMLSAV
Sbjct: 241 SCLSWLDLGGHKKAMNKTAKVLDEMLSKWIKEHREKKKNWGEGGVETEEQDFMDAMLSAV 300

Query: 301 DDDGGLCGFDADTITKATCLSMMLGGYTTMIATTWALSLLLNNQETLKKAQLELDEKVGT 360
           DDDGGLCGFDADTITKATCLSMMLGGYTTMIATTWALSLLLNNQETLKKAQLELDEKVGT
Sbjct: 301 DDDGGLCGFDADTITKATCLSMMLGGYTTMIATTWALSLLLNNQETLKKAQLELDEKVGT 360

Query: 361 SRQVKEADLKNLIYFQAIVKETLRLYPAAPLLIPHESIEDCMVSGYHIPARTRLLVNVQK 420
           SRQVKEADLKNLIYFQAIVKETLRLYPAAPLLIPHESIEDCMVSGYHIPARTRLLVNVQK
Sbjct: 361 SRQVKEADLKNLIYFQAIVKETLRLYPAAPLLIPHESIEDCMVSGYHIPARTRLLVNVQK 420

Query: 421 LQKDPLVWEDPCEFRPERFLTNDTKFDMQGQNMQLMPFGKGRRMCPGMSFALQLVHLTLA 480
           LQKDPLVWEDPCEFRPERFLTNDTKFDMQGQNMQLMPFGKGRRMCPGMSFALQLVHLTLA
Sbjct: 421 LQKDPLVWEDPCEFRPERFLTNDTKFDMQGQNMQLMPFGKGRRMCPGMSFALQLVHLTLA 480

Query: 481 SLLHEFEIDRLSEELLDMEE 501
           SLLHEFEIDRLSEELLDMEE
Sbjct: 481 SLLHEFEIDRLSEELLDMEE 500

[Close]

BLAST of CmaCh09G001830 vs. ExPASy TrEMBL
Match: A0A6J1F6F9 (cytochrome P450 CYP82D47-like OS=Cucurbita moschata OX=3662 GN=LOC111442567 PE=3 SV=1)

HSP 1 Score: 923.7 bits (2386), Expect = 3.3e-265
Identity = 458/500 (91.60%), Postives = 479/500 (95.80%), Query Frame = 0


Query: 1   MEITHILPAIFALLLLLYALFRIASPRSAAHLTNKTLPPEAAGRLPLIGHLHKLSATEPT 60
           ME+THIL A F LLL LYALFRI S RSAAH TN TLPPEAAGRLP+IGHLHKLSATEPT
Sbjct: 1   MELTHILAATFPLLLFLYALFRITSRRSAAHRTNNTLPPEAAGRLPVIGHLHKLSATEPT 60

Query: 61  HITLAKMADAYGPIFTLRLGMNTALVVSSWEIARECFTTNDRIFASRSKVVASELLGYNY 120
           HITLA+MADAYGPIFTL+LG+NTALVVSSWEIARECFTTNDRIFASRSKVVASELLGYNY
Sbjct: 61  HITLARMADAYGPIFTLKLGVNTALVVSSWEIARECFTTNDRIFASRSKVVASELLGYNY 120

Query: 121 AALGISPYGPYWRKVRKLATLELLTNHRLEQLQHIRICEVQNSIKKLYELCADSRNKGSG 180
           AALGISPYGPYWR VRKLAT+ELLTNHRLEQLQHIRICEVQNSI+KLYELCADSRNKGSG
Sbjct: 121 AALGISPYGPYWRHVRKLATVELLTNHRLEQLQHIRICEVQNSIEKLYELCADSRNKGSG 180

Query: 181 GKVRVEMRTWFGDVSLNTIFRMVVGNRFSAVFEGSGAEQYREALRAFFELFGAFVPSDSF 240
           GKVRVEMR WFGDVSLNTIFRMVVG RFSAVFEG+GAEQYREALRAFF+LF AFVPSDSF
Sbjct: 181 GKVRVEMRRWFGDVSLNTIFRMVVGKRFSAVFEGNGAEQYREALRAFFKLFAAFVPSDSF 240

Query: 241 SCLSWLDLGGHKKAMNKTAKVLDEMLSKWIKEHREKKKNWGEGGVETEEQDFMDAMLSAV 300
           SCLSWLDLGG+KKAMNKT KVLDEML+KWIKEHREKKKNWGEGGVETEEQDFMD MLSAV
Sbjct: 241 SCLSWLDLGGYKKAMNKTTKVLDEMLTKWIKEHREKKKNWGEGGVETEEQDFMDVMLSAV 300

Query: 301 DDDGGLCGFDADTITKATCLSMMLGGYTTMIATTWALSLLLNNQETLKKAQLELDEKVGT 360
           DDDGGLCGFDADT+TKATCLSMMLGGYTTMIATTWALSLLLNNQETL KAQLELDEKVG 
Sbjct: 301 DDDGGLCGFDADTVTKATCLSMMLGGYTTMIATTWALSLLLNNQETLNKAQLELDEKVGR 360

Query: 361 SRQVKEADLKNLIYFQAIVKETLRLYPAAPLLIPHESIEDCMVSGYHIPARTRLLVNVQK 420
           SRQVKE+D+KNLIY QAIVKETLRLYPAAP LIPHES+EDC +SGYHIPA+TRLLVNVQK
Sbjct: 361 SRQVKESDIKNLIYLQAIVKETLRLYPAAPFLIPHESVEDCTISGYHIPAKTRLLVNVQK 420

Query: 421 LQKDPLVWEDPCEFRPERFLTNDTKFDMQGQNMQLMPFGKGRRMCPGMSFALQLVHLTLA 480
           LQKDPLVWEDPCEFRPERFLTNDTKFDM+GQNMQLMPFG+GRRMCPGMSFALQLVHLTLA
Sbjct: 421 LQKDPLVWEDPCEFRPERFLTNDTKFDMKGQNMQLMPFGRGRRMCPGMSFALQLVHLTLA 480

Query: 481 SLLHEFEIDRLSEELLDMEE 501
           SLLHEF+I R SE+LLDMEE
Sbjct: 481 SLLHEFDIGRPSEKLLDMEE 500

[Close]

BLAST of CmaCh09G001830 vs. ExPASy TrEMBL
Match: A0A6J1F723 (cytochrome P450 CYP82D47-like OS=Cucurbita moschata OX=3662 GN=LOC111442948 PE=3 SV=1)

HSP 1 Score: 872.8 bits (2254), Expect = 6.7e-250
Identity = 443/503 (88.07%), Postives = 465/503 (92.45%), Query Frame = 0


Query: 1   MEITHILPAIFALLLLLYALFRIASPR-SAAHLTNKTLPPEAAGRLPLIGHLHKLSATEP 60
           ME+THILPA FALLL LYALFRIAS R SAAH +NK  PPEAAG LPLIGHLHKLSATEP
Sbjct: 1   MELTHILPATFALLLFLYALFRIASRRSSAAHRSNKRHPPEAAGALPLIGHLHKLSATEP 60

Query: 61  THITLAKMADAYGPIFTLRLGMNTALVVSSWEIARECFTTNDRIFASRSKVVASELLGYN 120
           THITLAKMADAYGPIFTLRLGMNTALVVSSWEIARECFTTND IFASRSKVVASELLGYN
Sbjct: 61  THITLAKMADAYGPIFTLRLGMNTALVVSSWEIARECFTTNDTIFASRSKVVASELLGYN 120

Query: 121 YAALGISPYGPYWRKVRKLATLELLTNHRLEQLQHIRICEVQNSIKKLYELCADSRNKGS 180
           YAALGISPYGPYWR VRKLATLELLTNHRLEQLQHIRICEVQNSIKKLYELCADSRNKGS
Sbjct: 121 YAALGISPYGPYWRHVRKLATLELLTNHRLEQLQHIRICEVQNSIKKLYELCADSRNKGS 180

Query: 181 GGKVRVEMRTWFGDVSLNTIFRMVVGNRFSAVFEGSGAEQYREALRAFFELFGAFVPSDS 240
           GGKVRVEMR WFGDVSLNTIFRMVVG RFSAVFEGSGAEQYREALRAFFELF AFVPSD 
Sbjct: 181 GGKVRVEMRRWFGDVSLNTIFRMVVGKRFSAVFEGSGAEQYREALRAFFELFAAFVPSDL 240

Query: 241 FSCLSWLDLGGHKKAMNKTAKVLDEMLSKWIKEHREKKKNWGEGGVETEEQDFMDAMLSA 300
           F  LSWLDLGG+KKAMNKTAKVLD+MLSKWI+EHREKKKNWGEGGVETEEQDFMD MLSA
Sbjct: 241 FPFLSWLDLGGYKKAMNKTAKVLDQMLSKWIEEHREKKKNWGEGGVETEEQDFMDLMLSA 300

Query: 301 VDDDGGLCGFDADTITKATCLSMMLGGY-TTMIATTWALSLLLNNQETLKKAQLELDEKV 360
           V DDGGLCG   DT+TKATCL+M+LGG  TT I  TWALSLLLN+QE LKKAQLELDEK+
Sbjct: 301 VHDDGGLCGIGGDTVTKATCLTMILGGLDTTTITMTWALSLLLNDQEALKKAQLELDEKI 360

Query: 361 GTSRQVKEADLKNLIYFQAIVKETLRLYPAAPLLIPHESIEDCMVSGYHIPARTRLLVNV 420
           G SRQVKE+D+KNL Y QAIVKETLRLYPAAPLLIPHES+EDC+VSGYHIPARTRLLVNV
Sbjct: 361 GRSRQVKESDVKNLTYLQAIVKETLRLYPAAPLLIPHESVEDCIVSGYHIPARTRLLVNV 420

Query: 421 QKLQKDPLVWEDPCEFRPERFLTNDTKFDMQGQNMQLMPFGKGRRMCPGMSFALQLVHLT 480
           QKLQKDPLVWEDPCEFRPERFL + TKFD++GQN QLMPFG GRR CPG+SF+LQL+HLT
Sbjct: 421 QKLQKDPLVWEDPCEFRPERFLISHTKFDLRGQNHQLMPFGSGRRKCPGISFSLQLMHLT 480

Query: 481 LASLLHEFEIDRLSEELLDMEEE 502
           LA+LLHEFEI R SE+LLD EEE
Sbjct: 481 LANLLHEFEIGRPSEKLLDKEEE 503

[Close]

BLAST of CmaCh09G001830 vs. ExPASy TrEMBL
Match: A0A6J1IF60 (cytochrome P450 82A3-like OS=Cucurbita maxima OX=3661 GN=LOC111476389 PE=3 SV=1)

HSP 1 Score: 832.4 bits (2149), Expect = 1.0e-237
Identity = 415/493 (84.18%), Postives = 450/493 (91.28%), Query Frame = 0


Query: 9   AIFALLLLLYALFRIASPRSAAHLTNKTLPPEAAGRLPLIGHLHKLSATEPTHITLAKMA 68
           AIFALLLLLYALFRIASPRSAA+ TNKTLPPEAAGRLPLIGHLHKLSATEPTHITLAKMA
Sbjct: 479 AIFALLLLLYALFRIASPRSAANRTNKTLPPEAAGRLPLIGHLHKLSATEPTHITLAKMA 538

Query: 69  DAYGPIFTLRLGMNTALVVSSWEIARECFTTNDRIFASRSKVVASELLGYNYAALGISPY 128
           D YGPIFTLRLGMNTALVVSSWEIARECFTTND IFASR K +A ELLGYN A + ISPY
Sbjct: 539 DVYGPIFTLRLGMNTALVVSSWEIARECFTTNDTIFASRPKGLAPELLGYNSAMMAISPY 598

Query: 129 GPYWRKVRKLATLELLTNHRLEQLQHIRICEVQNSIKKLYELCADSRNKGSGGKVRVEMR 188
           GPYWR VRKLATLELLTNHRLEQLQHIRI EVQ+SIKKLYELCADS +KGSGGKVRVEMR
Sbjct: 599 GPYWRHVRKLATLELLTNHRLEQLQHIRISEVQSSIKKLYELCADSTSKGSGGKVRVEMR 658

Query: 189 TWFGDVSLNTIFRMVVGNRFSAVFEGSGAEQYREALRAFFELFGAFVPSDSFSCLSWLDL 248
            WFGDVSLNTIFRMVVG RFSAVFEGSG EQYR ALR  FELFGAFVPSDSF CLSWLD+
Sbjct: 659 KWFGDVSLNTIFRMVVGKRFSAVFEGSGGEQYRRALRDVFELFGAFVPSDSFPCLSWLDV 718

Query: 249 GGHKKAMNKTAKVLDEMLSKWIKEHREKKKNWGEGGVETEEQDFMDAMLSAVDDDGGLCG 308
           GG+K+AMNKTAKVLDEMLSKWI+EHREKKKNWGEGGV+T+EQDFMD MLSAV DDGGLCG
Sbjct: 719 GGYKRAMNKTAKVLDEMLSKWIEEHREKKKNWGEGGVDTKEQDFMDVMLSAVGDDGGLCG 778

Query: 309 FDADTITKATCLSMMLGGY-TTMIATTWALSLLLNNQETLKKAQLELDEKVGTSRQVKEA 368
           FDADT+TKATC +++LG   TT+I  TWALSLLLN+ E L+K QLELDEKVG SRQVKE+
Sbjct: 779 FDADTVTKATCFAIILGASDTTIITMTWALSLLLNHPEALRKVQLELDEKVGRSRQVKES 838

Query: 369 DLKNLIYFQAIVKETLRLYPAAPLLIPHESIEDCMVSGYHIPARTRLLVNVQKLQKDPLV 428
           D+KNL+Y QAI+KETLRLYPAAPLLIPHES+EDC V GYHIPARTRL+VNVQKL KDPL+
Sbjct: 839 DVKNLLYLQAIMKETLRLYPAAPLLIPHESVEDCTVLGYHIPARTRLIVNVQKLHKDPLI 898

Query: 429 WEDPCEFRPERFLTNDTKFDMQGQNMQLMPFGKGRRMCPGMSFALQLVHLTLASLLHEFE 488
           WE+PCEFRPERFLT+DTKFDM+GQN QLMPFG GRRMCPG+ FAL+ +H  LA+LL +FE
Sbjct: 899 WENPCEFRPERFLTSDTKFDMRGQNQQLMPFGSGRRMCPGILFALRFMHFILANLLQKFE 958

Query: 489 IDRLSEELLDMEE 501
           I+R SE+LLDMEE
Sbjct: 959 IERPSEKLLDMEE 971

[Close]

BLAST of CmaCh09G001830 vs. NCBI nr
Match: XP_022976387.1 (cytochrome P450 CYP82D47-like isoform X2 [Cucurbita maxima])

HSP 1 Score: 993.8 bits (2568), Expect = 5.4e-286
Identity = 500/500 (100.00%), Postives = 500/500 (100.00%), Query Frame = 0


Query: 1   MEITHILPAIFALLLLLYALFRIASPRSAAHLTNKTLPPEAAGRLPLIGHLHKLSATEPT 60
           MEITHILPAIFALLLLLYALFRIASPRSAAHLTNKTLPPEAAGRLPLIGHLHKLSATEPT
Sbjct: 1   MEITHILPAIFALLLLLYALFRIASPRSAAHLTNKTLPPEAAGRLPLIGHLHKLSATEPT 60

Query: 61  HITLAKMADAYGPIFTLRLGMNTALVVSSWEIARECFTTNDRIFASRSKVVASELLGYNY 120
           HITLAKMADAYGPIFTLRLGMNTALVVSSWEIARECFTTNDRIFASRSKVVASELLGYNY
Sbjct: 61  HITLAKMADAYGPIFTLRLGMNTALVVSSWEIARECFTTNDRIFASRSKVVASELLGYNY 120

Query: 121 AALGISPYGPYWRKVRKLATLELLTNHRLEQLQHIRICEVQNSIKKLYELCADSRNKGSG 180
           AALGISPYGPYWRKVRKLATLELLTNHRLEQLQHIRICEVQNSIKKLYELCADSRNKGSG
Sbjct: 121 AALGISPYGPYWRKVRKLATLELLTNHRLEQLQHIRICEVQNSIKKLYELCADSRNKGSG 180

Query: 181 GKVRVEMRTWFGDVSLNTIFRMVVGNRFSAVFEGSGAEQYREALRAFFELFGAFVPSDSF 240
           GKVRVEMRTWFGDVSLNTIFRMVVGNRFSAVFEGSGAEQYREALRAFFELFGAFVPSDSF
Sbjct: 181 GKVRVEMRTWFGDVSLNTIFRMVVGNRFSAVFEGSGAEQYREALRAFFELFGAFVPSDSF 240

Query: 241 SCLSWLDLGGHKKAMNKTAKVLDEMLSKWIKEHREKKKNWGEGGVETEEQDFMDAMLSAV 300
           SCLSWLDLGGHKKAMNKTAKVLDEMLSKWIKEHREKKKNWGEGGVETEEQDFMDAMLSAV
Sbjct: 241 SCLSWLDLGGHKKAMNKTAKVLDEMLSKWIKEHREKKKNWGEGGVETEEQDFMDAMLSAV 300

Query: 301 DDDGGLCGFDADTITKATCLSMMLGGYTTMIATTWALSLLLNNQETLKKAQLELDEKVGT 360
           DDDGGLCGFDADTITKATCLSMMLGGYTTMIATTWALSLLLNNQETLKKAQLELDEKVGT
Sbjct: 301 DDDGGLCGFDADTITKATCLSMMLGGYTTMIATTWALSLLLNNQETLKKAQLELDEKVGT 360

Query: 361 SRQVKEADLKNLIYFQAIVKETLRLYPAAPLLIPHESIEDCMVSGYHIPARTRLLVNVQK 420
           SRQVKEADLKNLIYFQAIVKETLRLYPAAPLLIPHESIEDCMVSGYHIPARTRLLVNVQK
Sbjct: 361 SRQVKEADLKNLIYFQAIVKETLRLYPAAPLLIPHESIEDCMVSGYHIPARTRLLVNVQK 420

Query: 421 LQKDPLVWEDPCEFRPERFLTNDTKFDMQGQNMQLMPFGKGRRMCPGMSFALQLVHLTLA 480
           LQKDPLVWEDPCEFRPERFLTNDTKFDMQGQNMQLMPFGKGRRMCPGMSFALQLVHLTLA
Sbjct: 421 LQKDPLVWEDPCEFRPERFLTNDTKFDMQGQNMQLMPFGKGRRMCPGMSFALQLVHLTLA 480

Query: 481 SLLHEFEIDRLSEELLDMEE 501
           SLLHEFEIDRLSEELLDMEE
Sbjct: 481 SLLHEFEIDRLSEELLDMEE 500

[Close]

BLAST of CmaCh09G001830 vs. NCBI nr
Match: XP_022976386.1 (cytochrome P450 CYP82D47-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 993.8 bits (2568), Expect = 5.4e-286
Identity = 500/500 (100.00%), Postives = 500/500 (100.00%), Query Frame = 0


Query: 1   MEITHILPAIFALLLLLYALFRIASPRSAAHLTNKTLPPEAAGRLPLIGHLHKLSATEPT 60
           MEITHILPAIFALLLLLYALFRIASPRSAAHLTNKTLPPEAAGRLPLIGHLHKLSATEPT
Sbjct: 1   MEITHILPAIFALLLLLYALFRIASPRSAAHLTNKTLPPEAAGRLPLIGHLHKLSATEPT 60

Query: 61  HITLAKMADAYGPIFTLRLGMNTALVVSSWEIARECFTTNDRIFASRSKVVASELLGYNY 120
           HITLAKMADAYGPIFTLRLGMNTALVVSSWEIARECFTTNDRIFASRSKVVASELLGYNY
Sbjct: 61  HITLAKMADAYGPIFTLRLGMNTALVVSSWEIARECFTTNDRIFASRSKVVASELLGYNY 120

Query: 121 AALGISPYGPYWRKVRKLATLELLTNHRLEQLQHIRICEVQNSIKKLYELCADSRNKGSG 180
           AALGISPYGPYWRKVRKLATLELLTNHRLEQLQHIRICEVQNSIKKLYELCADSRNKGSG
Sbjct: 121 AALGISPYGPYWRKVRKLATLELLTNHRLEQLQHIRICEVQNSIKKLYELCADSRNKGSG 180

Query: 181 GKVRVEMRTWFGDVSLNTIFRMVVGNRFSAVFEGSGAEQYREALRAFFELFGAFVPSDSF 240
           GKVRVEMRTWFGDVSLNTIFRMVVGNRFSAVFEGSGAEQYREALRAFFELFGAFVPSDSF
Sbjct: 181 GKVRVEMRTWFGDVSLNTIFRMVVGNRFSAVFEGSGAEQYREALRAFFELFGAFVPSDSF 240

Query: 241 SCLSWLDLGGHKKAMNKTAKVLDEMLSKWIKEHREKKKNWGEGGVETEEQDFMDAMLSAV 300
           SCLSWLDLGGHKKAMNKTAKVLDEMLSKWIKEHREKKKNWGEGGVETEEQDFMDAMLSAV
Sbjct: 241 SCLSWLDLGGHKKAMNKTAKVLDEMLSKWIKEHREKKKNWGEGGVETEEQDFMDAMLSAV 300

Query: 301 DDDGGLCGFDADTITKATCLSMMLGGYTTMIATTWALSLLLNNQETLKKAQLELDEKVGT 360
           DDDGGLCGFDADTITKATCLSMMLGGYTTMIATTWALSLLLNNQETLKKAQLELDEKVGT
Sbjct: 301 DDDGGLCGFDADTITKATCLSMMLGGYTTMIATTWALSLLLNNQETLKKAQLELDEKVGT 360

Query: 361 SRQVKEADLKNLIYFQAIVKETLRLYPAAPLLIPHESIEDCMVSGYHIPARTRLLVNVQK 420
           SRQVKEADLKNLIYFQAIVKETLRLYPAAPLLIPHESIEDCMVSGYHIPARTRLLVNVQK
Sbjct: 361 SRQVKEADLKNLIYFQAIVKETLRLYPAAPLLIPHESIEDCMVSGYHIPARTRLLVNVQK 420

Query: 421 LQKDPLVWEDPCEFRPERFLTNDTKFDMQGQNMQLMPFGKGRRMCPGMSFALQLVHLTLA 480
           LQKDPLVWEDPCEFRPERFLTNDTKFDMQGQNMQLMPFGKGRRMCPGMSFALQLVHLTLA
Sbjct: 421 LQKDPLVWEDPCEFRPERFLTNDTKFDMQGQNMQLMPFGKGRRMCPGMSFALQLVHLTLA 480

Query: 481 SLLHEFEIDRLSEELLDMEE 501
           SLLHEFEIDRLSEELLDMEE
Sbjct: 481 SLLHEFEIDRLSEELLDMEE 500

[Close]

BLAST of CmaCh09G001830 vs. NCBI nr
Match: XP_022935742.1 (cytochrome P450 CYP82D47-like [Cucurbita moschata])

HSP 1 Score: 923.7 bits (2386), Expect = 6.9e-265
Identity = 458/500 (91.60%), Postives = 479/500 (95.80%), Query Frame = 0


Query: 1   MEITHILPAIFALLLLLYALFRIASPRSAAHLTNKTLPPEAAGRLPLIGHLHKLSATEPT 60
           ME+THIL A F LLL LYALFRI S RSAAH TN TLPPEAAGRLP+IGHLHKLSATEPT
Sbjct: 1   MELTHILAATFPLLLFLYALFRITSRRSAAHRTNNTLPPEAAGRLPVIGHLHKLSATEPT 60

Query: 61  HITLAKMADAYGPIFTLRLGMNTALVVSSWEIARECFTTNDRIFASRSKVVASELLGYNY 120
           HITLA+MADAYGPIFTL+LG+NTALVVSSWEIARECFTTNDRIFASRSKVVASELLGYNY
Sbjct: 61  HITLARMADAYGPIFTLKLGVNTALVVSSWEIARECFTTNDRIFASRSKVVASELLGYNY 120

Query: 121 AALGISPYGPYWRKVRKLATLELLTNHRLEQLQHIRICEVQNSIKKLYELCADSRNKGSG 180
           AALGISPYGPYWR VRKLAT+ELLTNHRLEQLQHIRICEVQNSI+KLYELCADSRNKGSG
Sbjct: 121 AALGISPYGPYWRHVRKLATVELLTNHRLEQLQHIRICEVQNSIEKLYELCADSRNKGSG 180

Query: 181 GKVRVEMRTWFGDVSLNTIFRMVVGNRFSAVFEGSGAEQYREALRAFFELFGAFVPSDSF 240
           GKVRVEMR WFGDVSLNTIFRMVVG RFSAVFEG+GAEQYREALRAFF+LF AFVPSDSF
Sbjct: 181 GKVRVEMRRWFGDVSLNTIFRMVVGKRFSAVFEGNGAEQYREALRAFFKLFAAFVPSDSF 240

Query: 241 SCLSWLDLGGHKKAMNKTAKVLDEMLSKWIKEHREKKKNWGEGGVETEEQDFMDAMLSAV 300
           SCLSWLDLGG+KKAMNKT KVLDEML+KWIKEHREKKKNWGEGGVETEEQDFMD MLSAV
Sbjct: 241 SCLSWLDLGGYKKAMNKTTKVLDEMLTKWIKEHREKKKNWGEGGVETEEQDFMDVMLSAV 300

Query: 301 DDDGGLCGFDADTITKATCLSMMLGGYTTMIATTWALSLLLNNQETLKKAQLELDEKVGT 360
           DDDGGLCGFDADT+TKATCLSMMLGGYTTMIATTWALSLLLNNQETL KAQLELDEKVG 
Sbjct: 301 DDDGGLCGFDADTVTKATCLSMMLGGYTTMIATTWALSLLLNNQETLNKAQLELDEKVGR 360

Query: 361 SRQVKEADLKNLIYFQAIVKETLRLYPAAPLLIPHESIEDCMVSGYHIPARTRLLVNVQK 420
           SRQVKE+D+KNLIY QAIVKETLRLYPAAP LIPHES+EDC +SGYHIPA+TRLLVNVQK
Sbjct: 361 SRQVKESDIKNLIYLQAIVKETLRLYPAAPFLIPHESVEDCTISGYHIPAKTRLLVNVQK 420

Query: 421 LQKDPLVWEDPCEFRPERFLTNDTKFDMQGQNMQLMPFGKGRRMCPGMSFALQLVHLTLA 480
           LQKDPLVWEDPCEFRPERFLTNDTKFDM+GQNMQLMPFG+GRRMCPGMSFALQLVHLTLA
Sbjct: 421 LQKDPLVWEDPCEFRPERFLTNDTKFDMKGQNMQLMPFGRGRRMCPGMSFALQLVHLTLA 480

Query: 481 SLLHEFEIDRLSEELLDMEE 501
           SLLHEF+I R SE+LLDMEE
Sbjct: 481 SLLHEFDIGRPSEKLLDMEE 500

[Close]

BLAST of CmaCh09G001830 vs. NCBI nr
Match: XP_023535911.1 (cytochrome P450 CYP82D47-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 920.6 bits (2378), Expect = 5.8e-264
Identity = 458/500 (91.60%), Postives = 478/500 (95.60%), Query Frame = 0


Query: 1   MEITHILPAIFALLLLLYALFRIASPRSAAHLTNKTLPPEAAGRLPLIGHLHKLSATEPT 60
           ME+THILPA FALLL LYALFRI S RSAAH TN TLPPEAAGRLP+IGHLHKLSATEPT
Sbjct: 1   MELTHILPATFALLLFLYALFRITSRRSAAHRTNNTLPPEAAGRLPVIGHLHKLSATEPT 60

Query: 61  HITLAKMADAYGPIFTLRLGMNTALVVSSWEIARECFTTNDRIFASRSKVVASELLGYNY 120
           HITLAKMADAYGPIFTLRLGMNT LVVSSWEIARECFTTNDR+FASRSKVVASELLGYNY
Sbjct: 61  HITLAKMADAYGPIFTLRLGMNTTLVVSSWEIARECFTTNDRVFASRSKVVASELLGYNY 120

Query: 121 AALGISPYGPYWRKVRKLATLELLTNHRLEQLQHIRICEVQNSIKKLYELCADSRNKGSG 180
           AALGISPYGPYWR VRKLAT+ELLTNHRLEQLQHIRICEVQNSI+KLYELCADSRNKGSG
Sbjct: 121 AALGISPYGPYWRHVRKLATVELLTNHRLEQLQHIRICEVQNSIEKLYELCADSRNKGSG 180

Query: 181 GKVRVEMRTWFGDVSLNTIFRMVVGNRFSAVFEGSGAEQYREALRAFFELFGAFVPSDSF 240
           GKVRVEMRTWFGDVSLNTIFRMVVG RFSAVFEG+GAEQYREAL AFFELF AFVPSDSF
Sbjct: 181 GKVRVEMRTWFGDVSLNTIFRMVVGKRFSAVFEGNGAEQYREALSAFFELFAAFVPSDSF 240

Query: 241 SCLSWLDLGGHKKAMNKTAKVLDEMLSKWIKEHREKKKNWGEGGVETEEQDFMDAMLSAV 300
           SCLSWLDLGG+KK+MNKTAKVLD+MLSKWI+EHREK KN GEGGVETE+QDFMD MLSAV
Sbjct: 241 SCLSWLDLGGYKKSMNKTAKVLDQMLSKWIEEHREKNKNLGEGGVETEDQDFMDVMLSAV 300

Query: 301 DDDGGLCGFDADTITKATCLSMMLGGYTTMIATTWALSLLLNNQETLKKAQLELDEKVGT 360
            DDGGLCG+DADT+TKATCLSMMLGGYTTMIATTWALSLLLNNQ+TLKKAQLELDEKVG 
Sbjct: 301 HDDGGLCGYDADTVTKATCLSMMLGGYTTMIATTWALSLLLNNQDTLKKAQLELDEKVGR 360

Query: 361 SRQVKEADLKNLIYFQAIVKETLRLYPAAPLLIPHESIEDCMVSGYHIPARTRLLVNVQK 420
           SRQVKE+D+KNLIY QAIVKETLRLYPAAP LIPHES+EDC VSGYHI A+TRLLVNVQK
Sbjct: 361 SRQVKESDVKNLIYLQAIVKETLRLYPAAPFLIPHESVEDCTVSGYHISAKTRLLVNVQK 420

Query: 421 LQKDPLVWEDPCEFRPERFLTNDTKFDMQGQNMQLMPFGKGRRMCPGMSFALQLVHLTLA 480
           LQKDP VWEDPCEFRPERFLTNDTKFDM+GQNMQLMPFG+GRRMCPGMSFALQLVHLTLA
Sbjct: 421 LQKDPFVWEDPCEFRPERFLTNDTKFDMKGQNMQLMPFGRGRRMCPGMSFALQLVHLTLA 480

Query: 481 SLLHEFEIDRLSEELLDMEE 501
           SLLHEFEIDR SEELLDMEE
Sbjct: 481 SLLHEFEIDRPSEELLDMEE 500

[Close]

BLAST of CmaCh09G001830 vs. NCBI nr
Match: KAG7024221.1 (Cytochrome P450 82A3, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 904.0 bits (2335), Expect = 5.6e-259
Identity = 451/490 (92.04%), Postives = 468/490 (95.51%), Query Frame = 0


Query: 1   MEITHILPAIFALLLLLYALFRIASPRSAAHLTNKTLPPEAAGRLPLIGHLHKLSATEPT 60
           ME+THIL A FALLL LYALFRI S RSAAH TN T PPEAAGRLP+IGHLHKLSATEPT
Sbjct: 1   MELTHILAATFALLLFLYALFRITSRRSAAHRTNNTHPPEAAGRLPVIGHLHKLSATEPT 60

Query: 61  HITLAKMADAYGPIFTLRLGMNTALVVSSWEIARECFTTNDRIFASRSKVVASELLGYNY 120
           HITLAKMADAYGPIFTL+LGMNTALVVSSWEIARECFTTNDRIFASRSKVVASELLGYNY
Sbjct: 61  HITLAKMADAYGPIFTLKLGMNTALVVSSWEIARECFTTNDRIFASRSKVVASELLGYNY 120

Query: 121 AALGISPYGPYWRKVRKLATLELLTNHRLEQLQHIRICEVQNSIKKLYELCADSRNKGSG 180
           AALGISPYGPYWR VRKLAT+ELLTNHRLEQLQHIRICEVQNSI+KLYELCADSRNKGSG
Sbjct: 121 AALGISPYGPYWRHVRKLATVELLTNHRLEQLQHIRICEVQNSIEKLYELCADSRNKGSG 180

Query: 181 GKVRVEMRTWFGDVSLNTIFRMVVGNRFSAVFEGSGAEQYREALRAFFELFGAFVPSDSF 240
           GKVRVEMR WFGDVSLNTIFRMVVG RFSAVFEG+GAEQYREALRAFFELF AFVPSDSF
Sbjct: 181 GKVRVEMRRWFGDVSLNTIFRMVVGKRFSAVFEGNGAEQYREALRAFFELFAAFVPSDSF 240

Query: 241 SCLSWLDLGGHKKAMNKTAKVLDEMLSKWIKEHRE-KKKNWGEGGVETEEQDFMDAMLSA 300
           SCLSWLDLGG+KKAMNKT KVLDEML+KWIKEHRE KKKNWGEGGVETEEQDFMD MLSA
Sbjct: 241 SCLSWLDLGGYKKAMNKTTKVLDEMLTKWIKEHREKKKKNWGEGGVETEEQDFMDLMLSA 300

Query: 301 VDDDGGLCGFDADTITKATCLSMMLGGYTTMIATTWALSLLLNNQETLKKAQLELDEKVG 360
           VDDDGGLCGFDADT+TKATCLSMMLGGYTTMIATTWALSLLLNN +TLKKAQLELDEKVG
Sbjct: 301 VDDDGGLCGFDADTVTKATCLSMMLGGYTTMIATTWALSLLLNNPKTLKKAQLELDEKVG 360

Query: 361 TSRQVKEADLKNLIYFQAIVKETLRLYPAAPLLIPHESIEDCMVSGYHIPARTRLLVNVQ 420
            S QVKE+D+KNL Y QAI+KETLRLYPAAP LIPHES+EDC VSGYHIPARTRLLVNVQ
Sbjct: 361 RSSQVKESDVKNLPYLQAIIKETLRLYPAAPFLIPHESVEDCTVSGYHIPARTRLLVNVQ 420

Query: 421 KLQKDPLVWEDPCEFRPERFLTNDTKFDMQGQNMQLMPFGKGRRMCPGMSFALQLVHLTL 480
           KLQKDPLVWEDPCEFRPERFLTNDTKFDM+GQNMQLMPFG+GRRMCPGMSFALQLVHLTL
Sbjct: 421 KLQKDPLVWEDPCEFRPERFLTNDTKFDMKGQNMQLMPFGRGRRMCPGMSFALQLVHLTL 480

Query: 481 ASLLHEFEID 490
           ASLLHEFEI+
Sbjct: 481 ASLLHEFEIE 490

[Close]

BLAST of CmaCh09G001830 vs. TAIR 10
Match: AT4G31940.1 (cytochrome P450, family 82, subfamily C, polypeptide 4 )

HSP 1 Score: 438.7 bits (1127), Expect = 6.3e-123
Identity = 229/476 (48.11%), Postives = 322/476 (67.65%), Query Frame = 0


Query: 39  PEAAGRLPLIGHLHKLSATEP-THITLAKMADAYGPIFTLRLGMNTALVVSSWEIARECF 98
           P  +G  P+IGHLH L   E   + TL KMAD YGP  +L+LG N A VVSS+E+A++CF
Sbjct: 33  PAPSGAWPIIGHLHLLGGKEQLLYRTLGKMADHYGPAMSLQLGSNEAFVVSSFEVAKDCF 92

Query: 99  TTNDRIFASRSKVVASELLGYNYAALGISPYGPYWRKVRKLATLELLTNHRLEQLQHIRI 158
           T ND+  ASR    A++ +GYN+A  G +PY  +WR++RK+AT+ELL+N RL+ L+H+R+
Sbjct: 93  TVNDKALASRPMTAAAKHMGYNFAVFGFAPYSAFWREMRKIATIELLSNRRLQMLKHVRV 152

Query: 159 CEVQNSIKKLYELCADSRNKGSGGKVRVEMRTWFGDVSLNTIFRMVVGNRFSAVFEGSG- 218
            E+   +K LY L       G    V V++++W  D++LN I RMV G R+   F G G 
Sbjct: 153 SEITMGVKDLYSLWF---KNGGTKPVMVDLKSWLEDMTLNMIVRMVAGKRY---FGGGGS 212

Query: 219 --------AEQYREALRAFFELFGAFVPSDSFSCLSWLDLGGHKKAMNKTAKVLDEMLSK 278
                   A Q ++A+  FF L G F  SD+F  LS+ DL GH+K M +T   LD +L +
Sbjct: 213 VSSEDTEEAMQCKKAIAKFFHLIGIFTVSDAFPTLSFFDLQGHEKEMKQTGSELDVILER 272

Query: 279 WIKEHREKKKNWGEGGVETEEQDFMDAMLSAVDDDGGL--CGFDADTITKATCLSMMLGG 338
           WI+ HR+++K     G +  + DF+D M+S + + G L    +DA+T  K+TCL+++LGG
Sbjct: 273 WIENHRQQRK---FSGTKENDSDFIDVMMS-LAEQGKLSHLQYDANTSIKSTCLALILGG 332

Query: 339 YTTMIAT-TWALSLLLNNQETLKKAQLELDEKVGTSRQVKEADLKNLIYFQAIVKETLRL 398
             T  +T TWA+SLLLNN+E LKKAQ E+D  VG  R V+++D++NL+Y QAI+KETLRL
Sbjct: 333 SDTSASTLTWAISLLLNNKEMLKKAQDEIDIHVGRDRNVEDSDIENLVYLQAIIKETLRL 392

Query: 399 YPAAPLLIPHESIEDCMVSGYHIPARTRLLVNVQKLQKDPLVWEDPCEFRPERFLTNDTK 458
           YPA PLL P E++EDC V+GY++P  TRL+VNV K+Q+DP V+ +P EFRPERF+T + K
Sbjct: 393 YPAGPLLGPREAMEDCTVAGYYVPCGTRLIVNVWKIQRDPKVYMEPNEFRPERFITGEAK 452

Query: 459 -FDMQGQNMQLMPFGKGRRMCPGMSFALQLVHLTLASLLHEFEIDRLSEELLDMEE 501
            FD++GQN +LMPFG GRR CPG S A+Q++HL LA  LH F++  + +  +DM E
Sbjct: 453 EFDVRGQNFELMPFGSGRRSCPGSSLAMQVLHLGLARFLHSFDVKTVMDMPVDMSE 498

[Close]

BLAST of CmaCh09G001830 vs. TAIR 10
Match: AT4G31970.1 (cytochrome P450, family 82, subfamily C, polypeptide 2 )

HSP 1 Score: 420.6 bits (1080), Expect = 1.8e-117
Identity = 222/472 (47.03%), Postives = 313/472 (66.31%), Query Frame = 0


Query: 39  PEAAGRLPLIGHLHKLSATEP-THITLAKMADAYGPIFTLRLGMNTALVVSSWEIARECF 98
           P  +G  P+IGHLH LS  E   + TL KMAD YGP  +LRLG +   VVSS+E+A++CF
Sbjct: 33  PAPSGAWPIIGHLHLLSGKEQLLYRTLGKMADQYGPAMSLRLGSSETFVVSSFEVAKDCF 92

Query: 99  TTNDRIFASRSKVVASELLGYNYAALGISPYGPYWRKVRKLATLELLTNHRLEQLQHIRI 158
           T ND+  ASR    A++ +GY+ A  G +PY  +WR++RK+ATLELL+N RL+ L+H+R+
Sbjct: 93  TVNDKALASRPITAAAKHMGYDCAVFGFAPYSAFWREMRKIATLELLSNRRLQMLKHVRV 152

Query: 159 CEVQNSIKKLYELCADSRNKGSGGKVRVEMRTWFGDVSLNTIFRMVVGNRF-----SAVF 218
            E+   ++ LY L      KG    V V++++W  D+SLN + RMV G R+      +  
Sbjct: 153 SEISMVMQDLYSLWV---KKGGSEPVMVDLKSWLEDMSLNMMVRMVAGKRYFGGGSLSPE 212

Query: 219 EGSGAEQYREALRAFFELFGAFVPSDSFSCLSWLDLGGHKKAMNKTAKVLDEMLSKWIKE 278
           +   A Q R+ +  FF L G F  SD+F  L W D  GH+K M +T + LD +L +WI+ 
Sbjct: 213 DAEEARQCRKGVANFFHLVGIFTVSDAFPKLGWFDFQGHEKEMKQTGRELDVILERWIEN 272

Query: 279 HREKKKNWGEGGVETEEQDFMDAMLSAVDDDGGLCGFDADTIT--KATCLSMMLGGYTTM 338
           HR+++K     G +  + DF+D MLS + + G       D IT  K+TCL+++LGG  T 
Sbjct: 273 HRQQRK---VSGTKHNDSDFVDVMLS-LAEQGKFSHLQHDAITSIKSTCLALILGGSETS 332

Query: 339 IAT-TWALSLLLNNQETLKKAQLELDEKVGTSRQVKEADLKNLIYFQAIVKETLRLYPAA 398
            +T TWA+SLLLNN++ LKKAQ E+D  VG  R V+++D++NL+Y QAI+KETLRLYPA 
Sbjct: 333 PSTLTWAISLLLNNKDMLKKAQDEIDIHVGRDRNVEDSDIENLVYIQAIIKETLRLYPAG 392

Query: 399 PLLIPHESIEDCMVSGYHIPARTRLLVNVQKLQKDPLVWEDPCEFRPERFLTNDTK-FDM 458
           PLL   E+IEDC V+GY++   TR+LVNV K+Q+DP V+ +P EFRPERF+T + K FD+
Sbjct: 393 PLLGHREAIEDCTVAGYNVRRGTRMLVNVWKIQRDPRVYMEPNEFRPERFITGEAKEFDV 452

Query: 459 QGQNMQLMPFGKGRRMCPGMSFALQLVHLTLASLLHEFEIDRLSEELLDMEE 501
           +GQN +LMPFG GRR CPG S A+Q++HL LA  L  F++  + +  +DM E
Sbjct: 453 RGQNFELMPFGSGRRSCPGSSLAMQVLHLGLARFLQSFDVKTVMDMPVDMTE 497

[Close]

BLAST of CmaCh09G001830 vs. TAIR 10
Match: AT4G31950.1 (cytochrome P450, family 82, subfamily C, polypeptide 3 )

HSP 1 Score: 403.3 bits (1035), Expect = 2.9e-112
Identity = 221/476 (46.43%), Postives = 308/476 (64.71%), Query Frame = 0


Query: 39  PEAAGRLPLIGHLHKLSATEP-THITLAKMADAYGPIFTLRLGMNTALVVSSWEIARECF 98
           P  +G  P+IGHLH L   E   + TL KMAD YGP  +LRLG +   V SS+E+A++CF
Sbjct: 33  PAPSGAWPIIGHLHLLGGKEQLLYRTLGKMADHYGPAMSLRLGSSETFVGSSFEVAKDCF 92

Query: 99  TTNDRIFASRSKVVASELLGYNYAALGISPYGPYWRKVRKLATLELLTNHRLEQLQHIRI 158
           T ND+  AS     A++ +GY            +W ++RK+A +ELL+N RL+ L ++R+
Sbjct: 93  TVNDKALAS-LMTAAAKHMGY-----------VFWLEMRKIAMIELLSNRRLQMLNNVRV 152

Query: 159 CEVQNSIKKLYELCADSRNKGSGGKVRVEMRTWFGDVSLNTIFRMVVGNRFSAVFEGSGA 218
            E+   +K LY L      KG    V V++++W  D+  N I RMV G R+   F G GA
Sbjct: 153 SEISMGVKDLYSLWV---KKGGSEPVMVDLKSWLEDMIANMIMRMVAGKRY---FGGGGA 212

Query: 219 E---------QYREALRAFFELFGAFVPSDSFSCLSWLDLGGHKKAMNKTAKVLDEMLSK 278
           E         Q+R+ +  FF L G F  SD+F  L WLDL GH+K M +T + LD +L +
Sbjct: 213 ESSEHTEEARQWRKGIAKFFHLVGIFTVSDAFPKLGWLDLQGHEKEMKQTRRELDVILER 272

Query: 279 WIKEHREKKKNWGEGGVETEEQDFMDAMLSAVDDDGGL--CGFDADTITKATCLSMMLGG 338
           WI+ HR+++K     G +  + DF+D MLS + + G L    +DA+T  K TCL+++LGG
Sbjct: 273 WIENHRQQRK---VSGTKHNDSDFVDVMLS-LAEQGKLSHLQYDANTCIKTTCLALILGG 332

Query: 339 YTTMIAT-TWALSLLLNNQETLKKAQLELDEKVGTSRQVKEADLKNLIYFQAIVKETLRL 398
             T  +T TWA+SLLLNN++ LKK Q E+D  VG  R V+++D+KNL+Y QAI+KETLRL
Sbjct: 333 SETSPSTLTWAISLLLNNKDMLKKVQDEIDIHVGRDRNVEDSDIKNLVYLQAIIKETLRL 392

Query: 399 YPAAPLLIPHESIEDCMVSGYHIPARTRLLVNVQKLQKDPLVWEDPCEFRPERFLTNDTK 458
           YPAAPLL   E++EDC V+GY++P  TRL+VNV K+Q+DP V+ +P EFRPERF+T + K
Sbjct: 393 YPAAPLLGHREAMEDCTVAGYNVPCGTRLIVNVWKIQRDPKVYMEPNEFRPERFITGEAK 452

Query: 459 -FDMQGQNMQLMPFGKGRRMCPGMSFALQLVHLTLASLLHEFEIDRLSEELLDMEE 501
            FD++GQN +LMPFG GRR CPG S A+Q++HL LA  LH FE+  + +  +DM E
Sbjct: 453 DFDVRGQNFELMPFGSGRRSCPGPSLAMQMLHLGLARFLHSFEVKTVLDRPVDMSE 486

[Close]

BLAST of CmaCh09G001830 vs. TAIR 10
Match: AT3G25180.1 (cytochrome P450, family 82, subfamily G, polypeptide 1 )

HSP 1 Score: 342.0 bits (876), Expect = 8.0e-94
Identity = 199/493 (40.37%), Postives = 285/493 (57.81%), Query Frame = 0


Query: 9   AIFALLLLLYALFRIASPRSAAHLTNKTLPPEAAGRLPLIGHLHKLSATEPTHITLAKMA 68
           ++F+L L+++    +    S   + + T+ PE  G LPL GHLH L   +     LA M+
Sbjct: 11  SLFSLALVIFGYIFLRKQLSRCEVDSSTI-PEPLGALPLFGHLHLLRGKKLLCKKLAAMS 70

Query: 69  DAYGPIFTLRLGMNTALVVSSWEIARECFTTNDRIFASRSKVVASELLGYNYAALGISPY 128
             +GPIF+L+LG    +V S  +  ++CFTTND   A+R  +     +GYN A+L ++PY
Sbjct: 71  QKHGPIFSLKLGFYRLVVASDPKTVKDCFTTNDLATATRPNIAFGRYVGYNNASLTLAPY 130

Query: 129 GPYWRKVRKLATLELLTNHRLEQLQHIRICEVQNSIKKLYELCADSRNKGSGGKVRVEMR 188
           G YWR++RK+ T+ L +NH +E L HIR  EV   IK LY        KG+GG   V++ 
Sbjct: 131 GDYWRELRKIVTVHLFSNHSIEMLGHIRSSEVNTLIKHLY--------KGNGGTSIVKID 190

Query: 189 TWFGDVSLNTIFRMVVGNRFSAVFEGSGAEQYREALRAFFELFGAFVPSDSFSCLSWLDL 248
             F  ++ N I R +VG R       S   +Y+EAL+    L    +  D    L WLD 
Sbjct: 191 MLFEFLTFNIILRKMVGKRIGFGEVNSDEWRYKEALKHCEYLAVIPMIGDVIPWLGWLDF 250

Query: 249 GGHKKAMNKTAKVLDEMLSKWIKEHREKKKNWGEGGVETEEQDFMDAMLSAVDDDGGLCG 308
             + + M +  K LD + +KW+ EH +K+        + +E+  MD +L  + +D  + G
Sbjct: 251 AKNSQ-MKRLFKELDSVNTKWLHEHLKKRSR----NEKDQERTIMDLLLDILPEDIVISG 310

Query: 309 FDADTITKATCLSMML-GGYTTMIATTWALSLLLNNQETLKKAQLELDEKVGTSRQVKEA 368
              D I KAT L++ L G  +T I  TWA+SLLLNN   L+ AQ E+D  VG  R ++E+
Sbjct: 311 HVRDVIVKATILALTLTGSDSTSITLTWAVSLLLNNPAALEAAQEEIDNSVGKGRWIEES 370

Query: 369 DLKNLIYFQAIVKETLRLYPAAPLLIPHESIEDCMVSGYHIPARTRLLVNVQKLQKDPLV 428
           D++NL Y QAIVKET RLYP APL    E+ EDC V GY +   TRLLVN+ KL +DP +
Sbjct: 371 DIQNLKYLQAIVKETHRLYPPAPLTGIREAREDCFVGGYRVEKGTRLLVNIWKLHRDPKI 430

Query: 429 WEDPCEFRPERFLTNDTKFDMQGQNMQLMPFGKGRRMCPGMSFALQLVHLTLASLLHEFE 488
           W DP  F+PERF+  + K   +  N + +PFG GRR CPG++  L++VH  LA LL  FE
Sbjct: 431 WPDPKTFKPERFM--EDKSQCEKSNFEYIPFGSGRRSCPGVNLGLRVVHFVLARLLQGFE 487

Query: 489 IDRLSEELLDMEE 501
           + ++S+E LDM E
Sbjct: 491 LHKVSDEPLDMAE 487

[Close]

BLAST of CmaCh09G001830 vs. TAIR 10
Match: AT2G25160.1 (cytochrome P450, family 82, subfamily F, polypeptide 1 )

HSP 1 Score: 322.0 bits (824), Expect = 8.5e-88
Identity = 189/497 (38.03%), Postives = 286/497 (57.55%), Query Frame = 0


Query: 10  IFALLLLLYALFRIASPRSAAHLTNKTLPPEAAGRLPLIGHLHKLSATEPTHITLAKMAD 69
           IF +L+L+ +  R  + +S A        P   G  PL+GHLH      PTH+T   MAD
Sbjct: 14  IFPVLILIKSRLRPKNKKSTA--------PMVPGAWPLLGHLHLFDTVNPTHVTFGAMAD 73

Query: 70  AYGPIFTLRLGMNTALVVSSWEIARECFTTNDRIFASRSKVVASELLGYNYAALGISPYG 129
            YGP+F  +LG    ++++S E+A+E +T +D++   R ++ AS+LLGYN + L  SPYG
Sbjct: 74  VYGPVFMAKLGSIKVMIINSKEVAKEIYTVHDKLL-ERPELTASKLLGYNDSFLTFSPYG 133

Query: 130 PYWRKVRKLATLELLTNHRLEQLQHIRICEVQNSIKKLYELCADSRNKGSGGKVRVEMRT 189
            YWR++RK+A  EL +   ++     R  E   + + LY +  + R K   G V V+M+ 
Sbjct: 134 LYWREIRKIAVSELFSTSGVDMHMVSRAREADLAFRALY-VRWEKRGKPKEG-VLVDMKQ 193

Query: 190 WFGDVSLNTIFRMVVGNRF---SAVFEGSGAEQYREALRAFFELFGAFVPSDSFSCLSWL 249
            F D++ N    MV G R+   +   E   A +  + +R F + F  F+ SD    L +L
Sbjct: 194 EFIDLTANISLMMVSGKRYFGENPNCEVKEARRCGKLIREFLDYFALFLLSDVAPVLGFL 253

Query: 250 DLGGHKKAMNKTAKVLDEMLSKWIKEHREKKKNWGEGGVETEEQDFMDAMLSAVDDDGGL 309
           D    K+ M +TAK LD++   WI+EH+ K+ + G       E D++D ++  +  D   
Sbjct: 254 D-WKTKRGMKRTAKGLDKVAEGWIEEHKNKRSDHGR-----SENDYLDILIKILGQDKIP 313

Query: 310 CGFDADTITKATCLSMML-GGYTTMIATTWALSLLLNNQETLKKAQLELDEKVGTSRQVK 369
              D  T  KA CL+++L G  T ++   WA+SLLLNN   L+KAQ ELD K+G  R V+
Sbjct: 314 GLSDTHTKIKALCLNLVLAGSETAIVVLVWAVSLLLNNPHVLRKAQEELDSKIGKERVVE 373

Query: 370 EADLKNLIYFQAIVKETLRLYPAAPLLIPHESIEDCMVS--GYHIPARTRLLVNVQKLQK 429
           E D+K+L+Y QAIVKET RLYP  PL+     +ED  ++    H+PA T+L+V+  K+ +
Sbjct: 374 ELDIKDLVYLQAIVKETFRLYPPVPLVAYRAVVEDFDIAFCKCHVPAGTQLMVSAWKIHR 433

Query: 430 DPLVWEDPCEFRPERFLTNDTKFDMQGQNMQLMPFGKGRRMCPGMSFALQLVHLTLASLL 489
           DP VW +P +F PERFLT++ + D+ GQ+ +  PFG GRR CP +   +++VH  L   L
Sbjct: 434 DPNVWSNPEQFEPERFLTSNRELDVGGQSYKFFPFGLGRRSCPAIPLGMRMVHYLLVRFL 493

Query: 490 HEFEIDRLSEELLDMEE 501
           H F++ R S + +DM E
Sbjct: 494 HSFDLARPSSQDVDMTE 493

The following BLAST results are available for this feature:

BLAST of CmaCh09G001830 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O49858	1.0e-122	46.31	Cytochrome P450 82A3 OS=Glycine max OX=3847 GN=CYP82A3 PE=2 SV=1	[more]
Q9SZ46	8.8e-122	48.11	Xanthotoxin 5-hydroxylase CYP82C4 OS=Arabidopsis thaliana OX=3702 GN=CYP82C4 PE=...	[more]
Q43068	1.7e-120	46.39	Cytochrome P450 82A1 (Fragment) OS=Pisum sativum OX=3888 GN=CYP82A1 PE=2 SV=2	[more]
A0A0N7F297	4.8e-120	49.25	Demethylepipodophyllotoxin synthase OS=Sinopodophyllum hexandrum OX=93608 GN=CYP...	[more]
O49394	2.5e-116	47.03	Xanthotoxin 5-hydroxylase CYP82C2 OS=Arabidopsis thaliana OX=3702 GN=CYP82C2 PE=...	[more]

BLAST of CmaCh09G001830 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1IGR6	2.6e-286	100.00	cytochrome P450 CYP82D47-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC11147...	[more]
A0A6J1IFL2	2.6e-286	100.00	cytochrome P450 CYP82D47-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC11147...	[more]
A0A6J1F6F9	3.3e-265	91.60	cytochrome P450 CYP82D47-like OS=Cucurbita moschata OX=3662 GN=LOC111442567 PE=3...	[more]
A0A6J1F723	6.7e-250	88.07	cytochrome P450 CYP82D47-like OS=Cucurbita moschata OX=3662 GN=LOC111442948 PE=3...	[more]
A0A6J1IF60	1.0e-237	84.18	cytochrome P450 82A3-like OS=Cucurbita maxima OX=3661 GN=LOC111476389 PE=3 SV=1	[more]

BLAST of CmaCh09G001830 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022976387.1	5.4e-286	100.00	cytochrome P450 CYP82D47-like isoform X2 [Cucurbita maxima]	[more]
XP_022976386.1	5.4e-286	100.00	cytochrome P450 CYP82D47-like isoform X1 [Cucurbita maxima]	[more]
XP_022935742.1	6.9e-265	91.60	cytochrome P450 CYP82D47-like [Cucurbita moschata]	[more]
XP_023535911.1	5.8e-264	91.60	cytochrome P450 CYP82D47-like [Cucurbita pepo subsp. pepo]	[more]
KAG7024221.1	5.6e-259	92.04	Cytochrome P450 82A3, partial [Cucurbita argyrosperma subsp. argyrosperma]	[more]

BLAST of CmaCh09G001830 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G31940.1	6.3e-123	48.11	cytochrome P450, family 82, subfamily C, polypeptide 4	[more]
AT4G31970.1	1.8e-117	47.03	cytochrome P450, family 82, subfamily C, polypeptide 2	[more]
AT4G31950.1	2.9e-112	46.43	cytochrome P450, family 82, subfamily C, polypeptide 3	[more]
AT3G25180.1	8.0e-94	40.37	cytochrome P450, family 82, subfamily G, polypeptide 1	[more]
AT2G25160.1	8.5e-88	38.03	cytochrome P450, family 82, subfamily F, polypeptide 1	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001128	Cytochrome P450	PRINTS	PR00385	P450	coord: 377..388 score: 46.11 coord: 465..476 score: 32.75 coord: 324..341 score: 30.93 coord: 456..465 score: 57.02
IPR001128	Cytochrome P450	PFAM	PF00067	p450	coord: 43..494 e-value: 1.2E-84 score: 284.8
IPR002401	Cytochrome P450, E-class, group I	PRINTS	PR00463	EP450I	coord: 455..465 score: 55.15 coord: 465..488 score: 32.7 coord: 68..87 score: 35.76 coord: 417..441 score: 40.17 coord: 194..212 score: 24.21 coord: 333..359 score: 33.29 coord: 92..113 score: 21.28 coord: 376..394 score: 38.63
IPR036396	Cytochrome P450 superfamily	GENE3D	1.10.630.10	Cytochrome P450	coord: 30..506 e-value: 1.1E-112 score: 379.1
IPR036396	Cytochrome P450 superfamily	SUPERFAMILY	48264	Cytochrome P450	coord: 43..500
None	No IPR available	PANTHER	PTHR47947:SF26	CYTOCHROME P450	coord: 37..501
None	No IPR available	PANTHER	PTHR47947	CYTOCHROME P450 82C3-RELATED	coord: 37..501
IPR017972	Cytochrome P450, conserved site	PROSITE	PS00086	CYTOCHROME_P450	coord: 458..467

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmaCh09G001830.1	CmaCh09G001830.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0004497	monooxygenase activity
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen