CmaCh08G012320 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh08G012320
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Description3-hydroxyacyl-CoA dehydrogenase
LocationCma_Chr08: 7561331 .. 7566369 (-)
RNA-Seq ExpressionCmaCh08G012320
SyntenyCmaCh08G012320
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
TAATATTTGCCATTTCGCGGGTTGATAATCGAGTTTCCGTTCCGTGTTCGACTCTACGAACGCTATTTCAGATTCAAAAGCGAAGCGACTTTCTTCTGAATCTGAAAGTTAATCCTGCGAATTTTTCATAATTTGTTGGAGAACGAAGCTCCAATCTGAGTTTTAGGAGATTGTATTGCTTTCTTCACTGAGAAATGGGAAGCCAAGCGAAGGGAAGAACTACTTTGGAGGTTGGAGCTGATGGAATCGCTCTAATCACCATCATTAATCCTCCCGTCAACTCACTTTCTTTCGATGGTATGATTTTGTGTTCTTTTACTTCCTTTTATGGCGTGTGATTTTACTGGATTCATCTATTTTGATCTTTGGTATGATGGCTACCTTATTCTGATTCTTTTTTCTTTTTTGTGTGAAGACTTTTTTTATTTTTATTTTTATTTTTATTTATAGATTTCGTAGTTTTAACATTTGTAGATGAATCAATTAAGGAAACTATCGAAGTTTCAGAAATAAATGAAGAGCTGCGTATACTATGATTATCTCCTAATTTTAGTTACGGTTCAATCAGTGACTGTTTGGTAATTGCTGATTCGATTTGGTTCACTGACTTCAAAGTTCTAGTGTAACAATGGAGGTATGGAGCTAGGTTCTAACACTGTACTTATCTGTGTGTGGGCGTTGTTCTAATTGAGCTGGGGTTTTTGCTGTGTATGAAAAACAACTGGAAAGGATTTGCGGTTAGTTGTAATTGATTGCGTTCAATTGCTGCAAATATGAAATATAGCGCACTGGATTTCACCCGGCCATGATAATTTTTTATTTGGAGACTCATTCTAATTGTTGGGAAATTGTATACTGAGATCTGGCTCTTGGGTGTTGTTTTTCTGAATAGAATTATTTAACTATTCATTTGTACTAAGAGTATTATTCATGTTTCTTTGTTCTGCACCTGTTCACACACGAATATAGTATTGTTCAGCTTGAAAGAGAGTTACGAACAGGCTTTACAAAGAGAAGATGTTAAGGCAATTGTTCTTACAGGTCAGCGTGTGAAATGGTTTTTATTGATGAGTTATGAAAGATATTAGAGATGTAAGAGCTAACTCTGTGTGTCAATGGAGCAGGTGCCAGGGGAAAGTTCTCTGGTGGGTTTGATATTACAGCCTTTGGTGGACTGCAAGGTGGAAAAGGTGATTTCTCTGTGCCCTATTGTCTCATAATTTACTCTGAAAACTTTTGTTGACATCTTTGAAATGGATTTTTAGCGAGGGAACCACGGCCTGGCTACATTTCCGTGGATGTGATCACCGACATTTTTGAAGGTATATTTCCACTCAGTTGCTTTAATCTTTGCTTGCATCCTCTAACTCTCATGGAGAGCATGAGAGAGATGGTTTTAGCTGAGTATTTTATTGTTAGCTATGATTTTCAGCCGCTAGAAAGCCTGCCGTTGCTGCAATTGACGGACTTGCTTTGGGTGGAGGGTTAGAGGTTGCAATGGTATGTCTTGCTACCTCTCTTAGACTATTAAATCTTGTAATTTTTTTTCGTTTTAAGATCTTTTCTTCAATTTTGATTTGAAGGCATGCCATGCACGAGTATCAACCAAAACGACGTTGTTAGGATTACCTGAACTTCAGCTTGGCCTAATTCCTGGTTTTGGAGGTATGTCTGTGTCACTGTTTCTTGGTGGTTGTTTTTATTTCTACTAATTGATTCTGTAGGCCACTTTCATGTATGTTCTCTCAACTGATCTTTACTTGAAGCTGTGGACAATTGTGATTTTACCAGTCCGCTTCCTCGTACTTCCATTGAAAATGAAGGATAACAGATGGCAGTTTGAGTTAGTCTGTCGGGTTCTGTATGGATTGAGTATTTGGATCTTGGGATTTTAATGAGATAGCATATAATGTACAATTTTTAGAGGACGGATAGTATACGTGTTATATATACCTAATATTTTCATTGGCTGCAAAGAATTTGTTTATTTTATCAAGAGGAGCCAACTTCTCCGAAACCTGCTCCTATGATTTTCCTTTTCACTTTTTCAAGCTTCTACATTGATCAGGAACACAGCGGCTTCCTCGTCTTGTTGGTCTTTCAAAGGCTTTAGAAATGATGCTGGTGAGATTAGTAGCTCATGCTTTGGATTTTTTATACGTTACTGTGGAAATAAATATTTTCATTAGCCTTACATATTTCCTGCTAATTTCTCTACCTGTCACTCTCTGCACGTCCATACTTGCTCTGCTTAAAAAGACATCCATGCCAGTCAAAGGAGAGGAGGCTTTTTCTCTGGGACTTGTGGATGCCATAGTCCCTTCTGAAGAGTTGATCAGCACTGCCCGCAAATTGGCTCTGGATATCTCAGAGCGGAGAAAACCATGGATTATCAGTCTTCACCGAACTGACAAGTTAGAGTCTCTTGGTGATGCTAGGGAGATATTTAAGTTTGCTCGGGCTCAGATACGGAAACAGGCTCCAAATCTTATGCATCCACTGGTTTGCATTGATGTTGTTGAAACCGGTGTAGTCTCAGGTCCCCGAGCTGGACTTTGGAAGGTTTCTATATTTCTAATGTAGTTATTTCCCTTTTTATTATTTTTAAAAAATAACACCATAACCTTCCTGAGTTATTTTTTATGCTTATGGCAGGAGATCGAAGATTTTCAAGTACTTCTGCATGCTGATACTAGTAAAAGCTTGATTAATATATTTTTTGCTCAGCGAGGAACAACGAAGGTTTGAGATGAAGAATTACTGCAAATTAAATGGTTGCTTAAGATCATCTTTACTACAGGATTGATAAAAACACACCCCTTGTTAATATGTTATGAGGATAGTCATTCGTTTACTTGTTGATATTGCTTCCTTTTAAAAAAATATAACAGGTACCTGGAGTTACTGATCTTGGGTTAAAGCCAAGACGTATTAAAAAAGTTGCTGTTGTTGGTGGTGGATTGATGGGATCTGGGATAGCTACAGCATTGGTTCTTAGCAACTATCCTGTGATTCTCAAAGAAGTGAACGATAAATTTTTGAAGGCTGGTCTTGACAGAGTCAGAGGTAAGCCTGGAATTTACTACTAATGATTGCATTTTTCGAATATTCTTATTCTATTCATTTATGGCTTGCCATGTTAACATGTTAGCTAATCTACAAAGCCGACTCCGTAAGGGCAAAATGACTCCAGAAAAATTTGAAAAAACTATTTCCCTACTCGATGGTGCCCTTGACTATGAAAGTTTCAAAGATGTTGATATGGTGATTGAGGTAATTCACGTCTTTCTTCTACACACACGAAAAGTAGCAATCATTAATATCAATTTGGTTGTGTTTGTAACAGGCTGTTATAGAAAACGTGTCTCTGAAGCAACAGATTTTTGTTGATCTAGAGAAATATTGCCCACCACATTGCATACTTGCTACCAATACGTCCACAATAGATTTGGAGCTGATTGGAGAGAGAACAAAATCTCATGATAGAATTGTCGGAGCTCATTTCTTTAGGTTTGTGAATTCGTATTTATCCTCATTCCTCTATTTTTCTTGGCTTAGCCACTTGGGCATTGAGTTTGTGCTTCTGATTGCAGTCCTGCACATGTCATGCCACTATTGGAAGTTGTTCGTACAAAAAGGACTGCTCCACAAGTAATTGTTGATTTGATAGATGTTGGAAAAAAGATAAAGAAAGCACCAGTTGTCGTTGGCAATTGCACTGGTTTTGCTGTCAATAGGATGTTTTTCCCATACACTCAAGCTGCACTGTTACTTGTAGAACATGGAGTAGACCCATATCAGATGGACAGGGCAGTTTTTAAGTTTGGAATGCCGATGGGCCCCTTCAGGTTTATAATACATAAAAGAAATTTGTGGTTCCCCTCCATGCTTTTTCTTTTTCGACCTTCCAACTTACTTTGGTTATTTTCCTTCAGATTGATTGATCTTGTTGGTTTTGGCGTGGCTATAGCAACCGGTGGTCAGTTTGTTCAGAATTTTCCTGATAGAACCTTCAAATCTATGATAATTCCTCTTATGCAAGAAGATAAGAGAGCGGGTAGAGTTTCACCCTCAAACTAGGGCGCTTGCATGTCTTGTTTTTCTGTCTTTCCACTTGGAAATATAGCTAGGGTGCCAAAGAACCCTACAATCACCGTTCAGTCTACTCGATAGTATTCGAGAGGGCGAATGACTCGGCAAAACCTTTCTCATGAAAATTGTCAGAGTTTTTTCCCCTTTCCAGTGTAAACGTTAATGATTAACTAAGACTCTTTCAGAGTCATGATGTTGTTCATTTTATCCATTCCCTGTAGACATTGACATTTGTTTGTTTAAAGAATAACCGTCATTGTGCATTTCAGGTGAAACAACCCAGAAAGGTTTCTATATCTACGATAAGAACCGCAAGGCTAAGCCAGATCCGGAGTTAAAGAAATATATTGAAAAGGCTAGGACCATGTCTGGCATTTCAATCGACCATAAGGTCTTGCTCTCTTTCTGCTCACGTGTTTCAATATTTCTGATCTGGTGTTCCTACCTTGAACTTGCATGAGTTGAGGATTAAATACCAAAATCATACAGCCATTAGTAAGATCTATGTAAGGATCTAATGTGATCGATGTGGATGCAGCTCACGAAGTTAACAGAAAAGGACATCATCGAGATGGTATTCTTCCCTGTGGTGAATGAAGCATGCCGAGTTCTCGCTGAAGGTATAGCAGTCAAAGCTGCAGACCTGGATATTGCTGGCGTAATGGGCATGGGTTTTCCACCTTACAGGTTGGTGAGAGTTCTGTAAATTCGTTCATTTGGTAGACTCAGTTAAATTATTGCTGTACAAAGTTTTGTTCTGGATGATCAGGGGAGGAGTGATGTTCTGGGCTGATTCTCTTGGATCAAAATACATCTATTCAAGATTGGAGGAGTGGTCAAAACATTATGGTGGATTCTTCAAGCCTTGTGCTTACTTGGCTGAAAGAGCTGCTCAGGGTGCAACGCTGGTGAGTAAACCACAACTCCAATCAGTAGAATTAAAATAA

mRNA sequence

TAATATTTGCCATTTCGCGGGTTGATAATCGAGTTTCCGTTCCGTGTTCGACTCTACGAACGCTATTTCAGATTCAAAAGCGAAGCGACTTTCTTCTGAATCTGAAAGTTAATCCTGCGAATTTTTCATAATTTGTTGGAGAACGAAGCTCCAATCTGAGTTTTAGGAGATTGTATTGCTTTCTTCACTGAGAAATGGGAAGCCAAGCGAAGGGAAGAACTACTTTGGAGGTTGGAGCTGATGGAATCGCTCTAATCACCATCATTAATCCTCCCGTCAACTCACTTTCTTTCGATGTATTGTTCAGCTTGAAAGAGAGTTACGAACAGGCTTTACAAAGAGAAGATGTTAAGGCAATTGTTCTTACAGGTGCCAGGGGAAAGTTCTCTGGTGGGTTTGATATTACAGCCTTTGGTGGACTGCAAGGTGGAAAAGCGAGGGAACCACGGCCTGGCTACATTTCCGTGGATGTGATCACCGACATTTTTGAAGCCGCTAGAAAGCCTGCCGTTGCTGCAATTGACGGACTTGCTTTGGGTGGAGGGTTAGAGGTTGCAATGGCATGCCATGCACGAGTATCAACCAAAACGACGTTGTTAGGATTACCTGAACTTCAGCTTGGCCTAATTCCTGGTTTTGGAGGAACACAGCGGCTTCCTCGTCTTGTTGGTCTTTCAAAGGCTTTAGAAATGATGCTGACATCCATGCCAGTCAAAGGAGAGGAGGCTTTTTCTCTGGGACTTGTGGATGCCATAGTCCCTTCTGAAGAGTTGATCAGCACTGCCCGCAAATTGGCTCTGGATATCTCAGAGCGGAGAAAACCATGGATTATCAGTCTTCACCGAACTGACAAGTTAGAGTCTCTTGGTGATGCTAGGGAGATATTTAAGTTTGCTCGGGCTCAGATACGGAAACAGGCTCCAAATCTTATGCATCCACTGGTTTGCATTGATGTTGTTGAAACCGGTGTAGTCTCAGGTCCCCGAGCTGGACTTTGGAAGGAGATCGAAGATTTTCAAGTACTTCTGCATGCTGATACTAGTAAAAGCTTGATTAATATATTTTTTGCTCAGCGAGGAACAACGAAGGTACCTGGAGTTACTGATCTTGGGTTAAAGCCAAGACGTATTAAAAAAGTTGCTGTTGTTGGTGGTGGATTGATGGGATCTGGGATAGCTACAGCATTGGTTCTTAGCAACTATCCTGTGATTCTCAAAGAAGTGAACGATAAATTTTTGAAGGCTGGTCTTGACAGAGTCAGAGCTAATCTACAAAGCCGACTCCGTAAGGGCAAAATGACTCCAGAAAAATTTGAAAAAACTATTTCCCTACTCGATGGTGCCCTTGACTATGAAAGTTTCAAAGATGTTGATATGGTGATTGAGGCTGTTATAGAAAACGTGTCTCTGAAGCAACAGATTTTTGTTGATCTAGAGAAATATTGCCCACCACATTGCATACTTGCTACCAATACGTCCACAATAGATTTGGAGCTGATTGGAGAGAGAACAAAATCTCATGATAGAATTGTCGGAGCTCATTTCTTTAGTCCTGCACATGTCATGCCACTATTGGAAGTTGTTCGTACAAAAAGGACTGCTCCACAAGTAATTGTTGATTTGATAGATGTTGGAAAAAAGATAAAGAAAGCACCAGTTGTCGTTGGCAATTGCACTGGTTTTGCTGTCAATAGGATGTTTTTCCCATACACTCAAGCTGCACTGTTACTTGTAGAACATGGAGTAGACCCATATCAGATGGACAGGGCAGTTTTTAAGTTTGGAATGCCGATGGGCCCCTTCAGATTGATTGATCTTGTTGGTTTTGGCGTGGCTATAGCAACCGGTGGTCAGTTTGTTCAGAATTTTCCTGATAGAACCTTCAAATCTATGATAATTCCTCTTATGCAAGAAGATAAGAGAGCGGGTGAAACAACCCAGAAAGGTTTCTATATCTACGATAAGAACCGCAAGGCTAAGCCAGATCCGGAGTTAAAGAAATATATTGAAAAGGCTAGGACCATGTCTGGCATTTCAATCGACCATAAGCTCACGAAGTTAACAGAAAAGGACATCATCGAGATGGTATTCTTCCCTGTGGTGAATGAAGCATGCCGAGTTCTCGCTGAAGGTATAGCAGTCAAAGCTGCAGACCTGGATATTGCTGGCGTAATGGGCATGGGTTTTCCACCTTACAGGGGAGGAGTGATGTTCTGGGCTGATTCTCTTGGATCAAAATACATCTATTCAAGATTGGAGGAGTGGTCAAAACATTATGGTGGATTCTTCAAGCCTTGTGCTTACTTGGCTGAAAGAGCTGCTCAGGGTGCAACGCTGGTGAGTAAACCACAACTCCAATCAGTAGAATTAAAATAA

Coding sequence (CDS)

ATGGGAAGCCAAGCGAAGGGAAGAACTACTTTGGAGGTTGGAGCTGATGGAATCGCTCTAATCACCATCATTAATCCTCCCGTCAACTCACTTTCTTTCGATGTATTGTTCAGCTTGAAAGAGAGTTACGAACAGGCTTTACAAAGAGAAGATGTTAAGGCAATTGTTCTTACAGGTGCCAGGGGAAAGTTCTCTGGTGGGTTTGATATTACAGCCTTTGGTGGACTGCAAGGTGGAAAAGCGAGGGAACCACGGCCTGGCTACATTTCCGTGGATGTGATCACCGACATTTTTGAAGCCGCTAGAAAGCCTGCCGTTGCTGCAATTGACGGACTTGCTTTGGGTGGAGGGTTAGAGGTTGCAATGGCATGCCATGCACGAGTATCAACCAAAACGACGTTGTTAGGATTACCTGAACTTCAGCTTGGCCTAATTCCTGGTTTTGGAGGAACACAGCGGCTTCCTCGTCTTGTTGGTCTTTCAAAGGCTTTAGAAATGATGCTGACATCCATGCCAGTCAAAGGAGAGGAGGCTTTTTCTCTGGGACTTGTGGATGCCATAGTCCCTTCTGAAGAGTTGATCAGCACTGCCCGCAAATTGGCTCTGGATATCTCAGAGCGGAGAAAACCATGGATTATCAGTCTTCACCGAACTGACAAGTTAGAGTCTCTTGGTGATGCTAGGGAGATATTTAAGTTTGCTCGGGCTCAGATACGGAAACAGGCTCCAAATCTTATGCATCCACTGGTTTGCATTGATGTTGTTGAAACCGGTGTAGTCTCAGGTCCCCGAGCTGGACTTTGGAAGGAGATCGAAGATTTTCAAGTACTTCTGCATGCTGATACTAGTAAAAGCTTGATTAATATATTTTTTGCTCAGCGAGGAACAACGAAGGTACCTGGAGTTACTGATCTTGGGTTAAAGCCAAGACGTATTAAAAAAGTTGCTGTTGTTGGTGGTGGATTGATGGGATCTGGGATAGCTACAGCATTGGTTCTTAGCAACTATCCTGTGATTCTCAAAGAAGTGAACGATAAATTTTTGAAGGCTGGTCTTGACAGAGTCAGAGCTAATCTACAAAGCCGACTCCGTAAGGGCAAAATGACTCCAGAAAAATTTGAAAAAACTATTTCCCTACTCGATGGTGCCCTTGACTATGAAAGTTTCAAAGATGTTGATATGGTGATTGAGGCTGTTATAGAAAACGTGTCTCTGAAGCAACAGATTTTTGTTGATCTAGAGAAATATTGCCCACCACATTGCATACTTGCTACCAATACGTCCACAATAGATTTGGAGCTGATTGGAGAGAGAACAAAATCTCATGATAGAATTGTCGGAGCTCATTTCTTTAGTCCTGCACATGTCATGCCACTATTGGAAGTTGTTCGTACAAAAAGGACTGCTCCACAAGTAATTGTTGATTTGATAGATGTTGGAAAAAAGATAAAGAAAGCACCAGTTGTCGTTGGCAATTGCACTGGTTTTGCTGTCAATAGGATGTTTTTCCCATACACTCAAGCTGCACTGTTACTTGTAGAACATGGAGTAGACCCATATCAGATGGACAGGGCAGTTTTTAAGTTTGGAATGCCGATGGGCCCCTTCAGATTGATTGATCTTGTTGGTTTTGGCGTGGCTATAGCAACCGGTGGTCAGTTTGTTCAGAATTTTCCTGATAGAACCTTCAAATCTATGATAATTCCTCTTATGCAAGAAGATAAGAGAGCGGGTGAAACAACCCAGAAAGGTTTCTATATCTACGATAAGAACCGCAAGGCTAAGCCAGATCCGGAGTTAAAGAAATATATTGAAAAGGCTAGGACCATGTCTGGCATTTCAATCGACCATAAGCTCACGAAGTTAACAGAAAAGGACATCATCGAGATGGTATTCTTCCCTGTGGTGAATGAAGCATGCCGAGTTCTCGCTGAAGGTATAGCAGTCAAAGCTGCAGACCTGGATATTGCTGGCGTAATGGGCATGGGTTTTCCACCTTACAGGGGAGGAGTGATGTTCTGGGCTGATTCTCTTGGATCAAAATACATCTATTCAAGATTGGAGGAGTGGTCAAAACATTATGGTGGATTCTTCAAGCCTTGTGCTTACTTGGCTGAAAGAGCTGCTCAGGGTGCAACGCTGGTGAGTAAACCACAACTCCAATCAGTAGAATTAAAATAA

Protein sequence

MGSQAKGRTTLEVGADGIALITIINPPVNSLSFDVLFSLKESYEQALQREDVKAIVLTGARGKFSGGFDITAFGGLQGGKAREPRPGYISVDVITDIFEAARKPAVAAIDGLALGGGLEVAMACHARVSTKTTLLGLPELQLGLIPGFGGTQRLPRLVGLSKALEMMLTSMPVKGEEAFSLGLVDAIVPSEELISTARKLALDISERRKPWIISLHRTDKLESLGDAREIFKFARAQIRKQAPNLMHPLVCIDVVETGVVSGPRAGLWKEIEDFQVLLHADTSKSLINIFFAQRGTTKVPGVTDLGLKPRRIKKVAVVGGGLMGSGIATALVLSNYPVILKEVNDKFLKAGLDRVRANLQSRLRKGKMTPEKFEKTISLLDGALDYESFKDVDMVIEAVIENVSLKQQIFVDLEKYCPPHCILATNTSTIDLELIGERTKSHDRIVGAHFFSPAHVMPLLEVVRTKRTAPQVIVDLIDVGKKIKKAPVVVGNCTGFAVNRMFFPYTQAALLLVEHGVDPYQMDRAVFKFGMPMGPFRLIDLVGFGVAIATGGQFVQNFPDRTFKSMIIPLMQEDKRAGETTQKGFYIYDKNRKAKPDPELKKYIEKARTMSGISIDHKLTKLTEKDIIEMVFFPVVNEACRVLAEGIAVKAADLDIAGVMGMGFPPYRGGVMFWADSLGSKYIYSRLEEWSKHYGGFFKPCAYLAERAAQGATLVSKPQLQSVELK
Homology
BLAST of CmaCh08G012320 vs. ExPASy Swiss-Prot
Match: Q39659 (Glyoxysomal fatty acid beta-oxidation multifunctional protein MFP-a OS=Cucumis sativus OX=3659 PE=1 SV=1)

HSP 1 Score: 1198.0 bits (3098), Expect = 0.0e+00
Identity = 591/714 (82.77%), Postives = 651/714 (91.18%), Query Frame = 0

Query: 1   MGSQAKGRTTLEVGADGIALITIINPPVNSLSFDVLFSLKESYEQALQREDVKAIVLTGA 60
           MGS AKGRT +EVG DG+A+ITIINPPVNSLSFDVLFSL++SYEQAL+R+DVKAIV+TGA
Sbjct: 1   MGSNAKGRTVMEVGTDGVAIITIINPPVNSLSFDVLFSLRDSYEQALRRDDVKAIVVTGA 60

Query: 61  RGKFSGGFDITAFGGLQGGKAREPRPGYISVDVITDIFEAARKPAVAAIDGLALGGGLEV 120
           +GKFSGGFDITAFG LQGGK  +P    IS+++ITDIFEAARKPAVAAIDGLALGGGLEV
Sbjct: 61  KGKFSGGFDITAFGVLQGGKGEQPNVRNISIEMITDIFEAARKPAVAAIDGLALGGGLEV 120

Query: 121 AMACHARVSTKTTLLGLPELQLGLIPGFGGTQRLPRLVGLSKALEMMLTSMPVKGEEAFS 180
           AMACHAR+ST T  LGLPELQLG+IPGFGGTQRLPRLVGLSKALEMMLTS P+KG+EA S
Sbjct: 121 AMACHARISTPTAQLGLPELQLGIIPGFGGTQRLPRLVGLSKALEMMLTSKPIKGQEAHS 180

Query: 181 LGLVDAIVPSEELISTARKLALDISERRKPWIISLHRTDKLESLGDAREIFKFARAQIRK 240
           LGLVDAIVP EELI+TAR+ AL+I ERR+PW+ SLHRTDKLESL +AR+IF  ARAQ +K
Sbjct: 181 LGLVDAIVPPEELINTARRWALEILERRRPWVHSLHRTDKLESLAEARKIFNLARAQAKK 240

Query: 241 QAPNLMHPLVCIDVVETGVVSGPRAGLWKEIEDFQVLLHADTSKSLINIFFAQRGTTKVP 300
           Q PNL H + CID VETGVVSGPRAGLWKE E+FQ LLH+DT KSLI+IFFAQR TTKVP
Sbjct: 241 QYPNLKHTIACIDAVETGVVSGPRAGLWKEAEEFQGLLHSDTCKSLIHIFFAQRSTTKVP 300

Query: 301 GVTDLGLKPRRIKKVAVVGGGLMGSGIATALVLSNYPVILKEVNDKFLKAGLDRVRANLQ 360
           GVTDLGL PR+IKKVA+VGGGLMGSGIATAL+LSNY V+LKEVNDKFL+AG+DRVRANLQ
Sbjct: 301 GVTDLGLVPRQIKKVAIVGGGLMGSGIATALILSNYHVVLKEVNDKFLQAGIDRVRANLQ 360

Query: 361 SRLRKGKMTPEKFEKTISLLDGALDYESFKDVDMVIEAVIENVSLKQQIFVDLEKYCPPH 420
           SR++KG MT EKFEK+ISLL G L+YESFKDVDMVIEAVIENVSLKQQIF DLEKYCPPH
Sbjct: 361 SRVKKGNMTNEKFEKSISLLKGVLNYESFKDVDMVIEAVIENVSLKQQIFSDLEKYCPPH 420

Query: 421 CILATNTSTIDLELIGERTKSHDRIVGAHFFSPAHVMPLLEVVRTKRTAPQVIVDLIDVG 480
           C+LATNTSTIDLELIGER KS DRI+GAHFFSPAH+MPLLE+VRTK TA QVIVDL+DVG
Sbjct: 421 CMLATNTSTIDLELIGERIKSRDRIIGAHFFSPAHIMPLLEIVRTKHTAAQVIVDLLDVG 480

Query: 481 KKIKKAPVVVGNCTGFAVNRMFFPYTQAALLLVEHGVDPYQMDRAVFKFGMPMGPFRLID 540
           K IKK PVVVGNCTGFAVNRMFFPY+QAA+LL EHGVDPYQ+DRA+ KFGMPMGPFRL D
Sbjct: 481 KNIKKTPVVVGNCTGFAVNRMFFPYSQAAILLAEHGVDPYQIDRAISKFGMPMGPFRLCD 540

Query: 541 LVGFGVAIATGGQFVQNFPDRTFKSMIIPLMQEDKRAGETTQKGFYIYDKNRKAKPDPEL 600
           LVGFGVA AT  QFVQ FP+RT+KSM+IPLMQEDK AGE+T+KGFY+YDKNRKA P+PEL
Sbjct: 541 LVGFGVAAATASQFVQAFPERTYKSMLIPLMQEDKNAGESTRKGFYVYDKNRKAGPNPEL 600

Query: 601 KKYIEKARTMSGISIDHKLTKLTEKDIIEMVFFPVVNEACRVLAEGIAVKAADLDIAGVM 660
           KKYIEKAR  SG+S+D KLTKL EKDI+EM+FFPVVNEACRVLAEGIAVKAADLDIAGVM
Sbjct: 601 KKYIEKARNSSGVSVDPKLTKLPEKDIVEMIFFPVVNEACRVLAEGIAVKAADLDIAGVM 660

Query: 661 GMGFPPYRGGVMFWADSLGSKYIYSRLEEWSKHYGGFFKPCAYLAERAAQGATL 715
           GMGFP YRGG+MFWADSLGS YIYSRLEEWSK YGGFFKPC YLAERA QGATL
Sbjct: 661 GMGFPSYRGGLMFWADSLGSNYIYSRLEEWSKQYGGFFKPCGYLAERAVQGATL 714

BLAST of CmaCh08G012320 vs. ExPASy Swiss-Prot
Match: Q9ZPI5 (Peroxisomal fatty acid beta-oxidation multifunctional protein MFP2 OS=Arabidopsis thaliana OX=3702 GN=MFP2 PE=1 SV=1)

HSP 1 Score: 1116.7 bits (2887), Expect = 0.0e+00
Identity = 542/722 (75.07%), Postives = 632/722 (87.53%), Query Frame = 0

Query: 1   MGSQAKGRTTLEVGADGIALITIINPPVNSLSFDVLFSLKESYEQALQREDVKAIVLTGA 60
           M S+ KG+T +EVG DG+A+IT+INPPVNSLSFDVL++LK +YE+AL R DVKAIV+TGA
Sbjct: 1   MDSRTKGKTVMEVGGDGVAVITLINPPVNSLSFDVLYNLKSNYEEALSRNDVKAIVITGA 60

Query: 61  RGKFSGGFDITAFGGLQGGKAREPRPGYISVDVITDIFEAARKPAVAAIDGLALGGGLEV 120
           +G+FSGGFDI+ FG +Q G  +EP+ GYIS+D+ITD+ EAARKP+VAAIDGLALGGGLE+
Sbjct: 61  KGRFSGGFDISGFGEMQKGNVKEPKAGYISIDIITDLLEAARKPSVAAIDGLALGGGLEL 120

Query: 121 AMACHARVSTKTTLLGLPELQLGLIPGFGGTQRLPRLVGLSKALEMMLTSMPVKGEEAFS 180
           AMACHAR+S     LGLPELQLG+IPGFGGTQRLPRLVGL+KALEM+LTS PVK EE  S
Sbjct: 121 AMACHARISAPAAQLGLPELQLGVIPGFGGTQRLPRLVGLTKALEMILTSKPVKAEEGHS 180

Query: 181 LGLVDAIVPSEELISTARKLALDISERRKPWIISLHRTDKLESLGDAREIFKFARAQIRK 240
           LGL+DA+VP  EL++TAR+ ALDI  RRKPW+ S+ +TDKL  LG+AREI  FA+AQ  K
Sbjct: 181 LGLIDAVVPPAELVTTARRWALDIVGRRKPWVSSVSKTDKLPPLGEAREILTFAKAQTLK 240

Query: 241 QAPNLMHPLVCIDVVETGVVSGPRAGLWKEIEDFQVLLHADTSKSLINIFFAQRGTTKVP 300
           +APN+ HPL+C+D +E G+VSGPRAGL KE E    ++  DT+K LI++FF+QRGT KVP
Sbjct: 241 RAPNMKHPLMCLDAIEVGIVSGPRAGLEKEAEVASQVVKLDTTKGLIHVFFSQRGTAKVP 300

Query: 301 GVTDLGLKPRRIKKVAVVGGGLMGSGIATALVLSNYPVILKEVNDKFLKAGLDRVRANLQ 360
           GVTD GL PR+IKKVA++GGGLMGSGIATAL+LSNYPVILKEVN+KFL+AG+ RV+ANLQ
Sbjct: 301 GVTDRGLVPRKIKKVAIIGGGLMGSGIATALILSNYPVILKEVNEKFLEAGIGRVKANLQ 360

Query: 361 SRLRKGKMTPEKFEKTISLLDGALDYESFKDVDMVIEAVIENVSLKQQIFVDLEKYCPPH 420
           SR+RKG M+ EKFEKT+SLL G+LDYESF+DVDMVIEAVIEN+SLKQQIF DLEKYCP H
Sbjct: 361 SRVRKGSMSQEKFEKTMSLLKGSLDYESFRDVDMVIEAVIENISLKQQIFADLEKYCPQH 420

Query: 421 CILATNTSTIDLELIGERTKSHDRIVGAHFFSPAHVMPLLEVVRTKRTAPQVIVDLIDVG 480
           CILA+NTSTIDL  IGERTKS DRIVGAHFFSPAH+MPLLE+VRT  T+ QVIVDL+DVG
Sbjct: 421 CILASNTSTIDLNKIGERTKSQDRIVGAHFFSPAHIMPLLEIVRTNHTSAQVIVDLLDVG 480

Query: 481 KKIKKAPVVVGNCTGFAVNRMFFPYTQAALLLVEHGVDPYQMDRAVFKFGMPMGPFRLID 540
           KKIKK PVVVGNCTGFAVNRMFFPYTQAA+ LVE G DPY +DRA+ KFGMPMGPFRL D
Sbjct: 481 KKIKKTPVVVGNCTGFAVNRMFFPYTQAAMFLVECGADPYLIDRAISKFGMPMGPFRLCD 540

Query: 541 LVGFGVAIATGGQFVQNFPDRTFKSMIIPLMQEDKRAGETTQKGFYIYDKNRKAKPDPEL 600
           LVGFGVAIAT  QF++NF +RT+KSMIIPLMQEDKRAGE T+KGFY+YD  RKAKPDPEL
Sbjct: 541 LVGFGVAIATATQFIENFSERTYKSMIIPLMQEDKRAGEATRKGFYLYDDKRKAKPDPEL 600

Query: 601 KKYIEKARTMSGISIDHKLTKLTEKDIIEMVFFPVVNEACRVLAEGIAVKAADLDIAGVM 660
           KKYIEKAR++SG+ +D KL  L+EKDIIEM FFPVVNEACRV AEGIAVKAADLDIAG+M
Sbjct: 601 KKYIEKARSISGVKLDPKLANLSEKDIIEMTFFPVVNEACRVFAEGIAVKAADLDIAGIM 660

Query: 661 GMGFPPYRGGVMFWADSLGSKYIYSRLEEWSKHYGGFFKPCAYLAERAAQGATLVSKPQL 720
           GMGFPPYRGG+MFWADS+GSKYIYSRL+EWSK YG FFKPCA+LAER ++G  L+S P  
Sbjct: 661 GMGFPPYRGGIMFWADSIGSKYIYSRLDEWSKAYGEFFKPCAFLAERGSKG-VLLSAPVK 720

Query: 721 QS 723
           Q+
Sbjct: 721 QA 721

BLAST of CmaCh08G012320 vs. ExPASy Swiss-Prot
Match: O49809 (Glyoxysomal fatty acid beta-oxidation multifunctional protein MFP-a OS=Brassica napus OX=3708 PE=2 SV=2)

HSP 1 Score: 1110.5 bits (2871), Expect = 0.0e+00
Identity = 540/722 (74.79%), Postives = 634/722 (87.81%), Query Frame = 0

Query: 1   MGSQAKGRTTLEVGADGIALITIINPPVNSLSFDVLFSLKESYEQALQREDVKAIVLTGA 60
           M S+ KG TT+EVGADG+A+IT+INPPVNSLSFDVL+SLK +YE+AL R DVKAIV+TGA
Sbjct: 1   MASRTKGTTTIEVGADGVAVITLINPPVNSLSFDVLYSLKSNYEEALSRNDVKAIVVTGA 60

Query: 61  RGKFSGGFDITAFGGLQGGKAREPRPGYISVDVITDIFEAARKPAVAAIDGLALGGGLEV 120
           +GKFSGGFDI+ FG +Q G  +EP+ GYIS+D++TD+ EAA+KP+VAAIDGLALGGGLE+
Sbjct: 61  KGKFSGGFDISGFGEIQKGTMKEPKVGYISIDILTDLLEAAKKPSVAAIDGLALGGGLEL 120

Query: 121 AMACHARVSTKTTLLGLPELQLGLIPGFGGTQRLPRLVGLSKALEMMLTSMPVKGEEAFS 180
           +MACHAR+S     LGLPELQLG+IPGFGGTQRLPRLVGL+KALEM+LTS PVK EE  S
Sbjct: 121 SMACHARISAPGAQLGLPELQLGVIPGFGGTQRLPRLVGLTKALEMILTSKPVKAEEGHS 180

Query: 181 LGLVDAIVPSEELISTARKLALDISERRKPWIISLHRTDKLESLGDAREIFKFARAQIRK 240
           LGL+DA+VP  EL++ AR+ ALDI+ERRKPW+ S+ +TDKL  LG+AREI KFA+ Q R+
Sbjct: 181 LGLIDAVVPPAELLNAARRWALDIAERRKPWVSSVLKTDKLPPLGEAREILKFAKDQTRR 240

Query: 241 QAPNLMHPLVCIDVVETGVVSGPRAGLWKEIEDFQVLLHADTSKSLINIFFAQRGTTKVP 300
           QAPN+ HPL+C++ VE G+VSG RAGL KE +    +++ DT+K LI++FF+QRGTTKVP
Sbjct: 241 QAPNMKHPLMCLEAVEVGIVSGSRAGLEKEAQVGSEVINLDTTKGLIHVFFSQRGTTKVP 300

Query: 301 GVTDLGLKPRRIKKVAVVGGGLMGSGIATALVLSNYPVILKEVNDKFLKAGLDRVRANLQ 360
           GVTD GL PR+I KVA++GGGLMGSGIATAL+LSNY VILKEVN+KFL+AG+ RV+ANLQ
Sbjct: 301 GVTDRGLVPRKINKVAIIGGGLMGSGIATALILSNYSVILKEVNEKFLEAGIGRVKANLQ 360

Query: 361 SRLRKGKMTPEKFEKTISLLDGALDYESFKDVDMVIEAVIENVSLKQQIFVDLEKYCPPH 420
           SR++KGKM+ EKFEKT+SLL G+LDYESF+DVDMVIEAVIEN+SLKQQIF DLEKYCP H
Sbjct: 361 SRVKKGKMSKEKFEKTMSLLKGSLDYESFRDVDMVIEAVIENISLKQQIFADLEKYCPQH 420

Query: 421 CILATNTSTIDLELIGERTKSHDRIVGAHFFSPAHVMPLLEVVRTKRTAPQVIVDLIDVG 480
           CILA+NTSTIDL  IGERTKS DRI+GAHFFSPAHVMPLLE+VRT  T+ QVIVDL+DVG
Sbjct: 421 CILASNTSTIDLNKIGERTKSQDRIIGAHFFSPAHVMPLLEIVRTNHTSAQVIVDLLDVG 480

Query: 481 KKIKKAPVVVGNCTGFAVNRMFFPYTQAALLLVEHGVDPYQMDRAVFKFGMPMGPFRLID 540
           KKI+K PVVVGNCTGFAVNRMFFPYTQAA+ LVEHG DPY +D+AV KFGMPMGPFRL D
Sbjct: 481 KKIRKTPVVVGNCTGFAVNRMFFPYTQAAMFLVEHGTDPYLIDKAVSKFGMPMGPFRLCD 540

Query: 541 LVGFGVAIATGGQFVQNFPDRTFKSMIIPLMQEDKRAGETTQKGFYIYDKNRKAKPDPEL 600
           LVGFGVAIAT  QF++NFP+RT+KSMIIPLMQEDKRAGE T+KGFY+YD  RKAKPDPE+
Sbjct: 541 LVGFGVAIATATQFIENFPERTYKSMIIPLMQEDKRAGEATRKGFYLYDDRRKAKPDPEI 600

Query: 601 KKYIEKARTMSGISIDHKLTKLTEKDIIEMVFFPVVNEACRVLAEGIAVKAADLDIAGVM 660
           K YI+KAR++SG   D KL KL+EK+IIEM FFPVVNEACRV AEGIAVKAADLDIAG+ 
Sbjct: 601 KNYIDKARSVSGAKPDPKLEKLSEKEIIEMTFFPVVNEACRVFAEGIAVKAADLDIAGIF 660

Query: 661 GMGFPPYRGGVMFWADSLGSKYIYSRLEEWSKHYGGFFKPCAYLAERAAQGATLVSKPQL 720
           GMGFPPYRGG+MFWADS+GSKYIYS+LEEWSK YG FFKPCA+LAER ++GA L S P  
Sbjct: 661 GMGFPPYRGGIMFWADSIGSKYIYSKLEEWSKAYGEFFKPCAFLAERGSKGAPL-SAPLE 720

Query: 721 QS 723
           QS
Sbjct: 721 QS 721

BLAST of CmaCh08G012320 vs. ExPASy Swiss-Prot
Match: Q9ZPI6 (Peroxisomal fatty acid beta-oxidation multifunctional protein AIM1 OS=Arabidopsis thaliana OX=3702 GN=AIM1 PE=1 SV=1)

HSP 1 Score: 814.3 bits (2102), Expect = 1.1e-234
Identity = 404/707 (57.14%), Postives = 529/707 (74.82%), Query Frame = 0

Query: 10  TLEVGADGIALITIINPPVNSLSFDVLFSLKESYEQALQREDVKAIVLTGARGKFSGGFD 69
           T+EVG DG+A+ITI NPPVNSL+  ++  LKE +  A QR DVKAIVL G  G+FSGGFD
Sbjct: 8   TMEVGNDGVAVITISNPPVNSLASPIISGLKEKFRDANQRNDVKAIVLIGNNGRFSGGFD 67

Query: 70  ITAFGGLQ--GGKAREPRPGYISVDVITDIFEAARKPAVAAIDGLALGGGLEVAMACHAR 129
           I  F  +   G  +  P    +SV+++ ++ E +RKP VAA++GLALGGGLE+AMACHAR
Sbjct: 68  INVFQQVHKTGDLSLMPE---VSVELVCNLMEDSRKPVVAAVEGLALGGGLELAMACHAR 127

Query: 130 VSTKTTLLGLPELQLGLIPGFGGTQRLPRLVGLSKALEMMLTSMPVKGEEAFSLGLVDAI 189
           V+     LGLPEL LG+IPGFGGTQRLPRLVGL+KA +M+L S  +  EE   LGL+DA+
Sbjct: 128 VAAPKAQLGLPELTLGVIPGFGGTQRLPRLVGLAKATDMILLSKSISSEEGHKLGLIDAL 187

Query: 190 VPSEELISTARKLALDISERRKPWIISLHRTDKLESLGDAREIFKFARAQIRKQAPNLMH 249
           VP  +++ST+RK ALDI+E RKP++ SLHRTDK+ SL +AR I K +R   +K APN+  
Sbjct: 188 VPPGDVLSTSRKWALDIAEGRKPFLQSLHRTDKIGSLSEARAILKNSRQLAKKIAPNMPQ 247

Query: 250 PLVCIDVVETGVVSGPRAGLWKEIEDFQVLLHADTSKSLINIFFAQRGTTKVPGVTDLGL 309
              CI+V+E G++ G  +G+ KE E F+ L+ +DT+K L+++FFAQR T+KVP VTD+GL
Sbjct: 248 HHACIEVIEEGIIHGGYSGVLKEAEVFKQLVLSDTAKGLVHVFFAQRATSKVPNVTDVGL 307

Query: 310 KPRRIKKVAVVGGGLMGSGIATALVLSNYPVILKEVNDKFLKAGLDRVRANLQSRLRKGK 369
           KPR IKKVAV+GGGLMGSGIATAL+LSN  V+LKE+N +FL  G+  V AN++S + +GK
Sbjct: 308 KPRPIKKVAVIGGGLMGSGIATALLLSNIRVVLKEINSEFLMKGIKSVEANMKSLVSRGK 367

Query: 370 MTPEKFEKTISLLDGALDYESFKDVDMVIEAVIENVSLKQQIFVDLEKYCPPHCILATNT 429
           +T +K  K +SL  G LDY  F DVDMVIEAVIEN+ LKQ IF ++EK C PHCILA+NT
Sbjct: 368 LTQDKAGKALSLFKGVLDYTEFNDVDMVIEAVIENIQLKQNIFKEIEKVCSPHCILASNT 427

Query: 430 STIDLELIGERTKSHDRIVGAHFFSPAHVMPLLEVVRTKRTAPQVIVDLIDVGKKIKKAP 489
           STIDL++IGE+T S DRIVGAHFFSPAH+MPLLE+VR+K T+ QVI+DL+ VGK IKK P
Sbjct: 428 STIDLDVIGEKTNSKDRIVGAHFFSPAHLMPLLEIVRSKNTSAQVILDLMAVGKAIKKVP 487

Query: 490 VVVGNCTGFAVNRMFFPYTQAALLLVEHGVDPYQMDRAVFKFGMPMGPFRLIDLVGFGVA 549
           VVVGNC GFAVNR FFPY+QAA +L   GVD +++D  +  FG+P+GPF+L DL G G+ 
Sbjct: 488 VVVGNCIGFAVNRTFFPYSQAAHMLANLGVDLFRIDSVITSFGLPLGPFQLGDLAGHGIG 547

Query: 550 IATGGQFVQNFPDRTFKSMIIPLMQEDKRAGETTQKGFYIYDKNRKAKPDPELKKYIEKA 609
           +A G  + + + DR F+S +  L+ +  R G+   +G+YIY+K  K KPDP +   +EK+
Sbjct: 548 LAVGPIYAKVYGDRMFRSPMTELLLKSGRNGKINGRGYYIYEKGSKPKPDPSVLSIVEKS 607

Query: 610 RTMSGISIDHKLTKLTEKDIIEMVFFPVVNEACRVLAEGIAVKAADLDIAGVMGMGFPPY 669
           R ++ I    K   +T+K+I+EM+ FPVVNEACRVL EG+ ++A+DLDIA V+GM FP Y
Sbjct: 608 RKLTNIMPGGKPISVTDKEIVEMILFPVVNEACRVLDEGVVIRASDLDIASVLGMSFPSY 667

Query: 670 RGGVMFWADSLGSKYIYSRLEEWSKHYGGFFKPCAYLAERAAQGATL 715
           RGG++FWAD++G KYIY RL++ S+ YG FFKP  YL ERA  G  L
Sbjct: 668 RGGIVFWADTVGPKYIYERLKKLSETYGSFFKPSRYLEERAMNGMLL 711

BLAST of CmaCh08G012320 vs. ExPASy Swiss-Prot
Match: Q8W1L6 (Peroxisomal fatty acid beta-oxidation multifunctional protein OS=Oryza sativa subsp. japonica OX=39947 GN=MFP PE=1 SV=2)

HSP 1 Score: 814.3 bits (2102), Expect = 1.1e-234
Identity = 404/707 (57.14%), Postives = 528/707 (74.68%), Query Frame = 0

Query: 8   RTTLEVGADGIALITIINPPVNSLSFDVLFSLKESYEQALQREDVKAIVLTGARGKFSGG 67
           R T+EVGADG+A++TI NPPVN+L   ++  LKE Y +A+ R+DVKAIVLTGA GKF GG
Sbjct: 6   RVTMEVGADGVAVVTICNPPVNALHPIIIQGLKEKYAEAMDRDDVKAIVLTGAGGKFCGG 65

Query: 68  FDITAFGGLQGGKAREPRPGYISVDVITDIFEAARKPAVAAIDGLALGGGLEVAMACHAR 127
           FDI  F  +         P  +SV++++++ EA +KP+VAAI GLALGGGLE+ M CHAR
Sbjct: 66  FDINVFTEVHKTGNVSLMPD-VSVELVSNLMEAGKKPSVAAIQGLALGGGLELTMGCHAR 125

Query: 128 VSTKTTLLGLPELQLGLIPGFGGTQRLPRLVGLSKALEMMLTSMPVKGEEAFSLGLVDAI 187
           +ST    LGLPEL LG+IPGFGGTQRLPRLVGL KA+EMML S  +  +E    GLVDA+
Sbjct: 126 ISTPEAQLGLPELTLGIIPGFGGTQRLPRLVGLPKAIEMMLQSKFITAKEGKEGGLVDAL 185

Query: 188 VPSEELISTARKLALDISERRKPWIISLHRTDKLESLGDAREIFKFARAQIRKQAPNLMH 247
              +ELI  +R  AL+I+  RKPWI SL RTD+L SL +AR +   AR Q +K A NL  
Sbjct: 186 CSPDELIKMSRLWALEIANYRKPWIRSLARTDRLGSLSEARSVLNSARQQAKKVAANLPQ 245

Query: 248 PLVCIDVVETGVVSGPRAGLWKEIEDFQVLLHADTSKSLINIFFAQRGTTKVPGVTDLGL 307
              C+DV+E GV+ G  AG+ KE + F+ L+ + TSK+L++ FFAQR TTKVPGVTD+ L
Sbjct: 246 HQACLDVMEEGVLCGGHAGVLKEAKVFKELVLSPTSKALVHAFFAQRLTTKVPGVTDVQL 305

Query: 308 KPRRIKKVAVVGGGLMGSGIATALVLSNYPVILKEVNDKFLKAGLDRVRANLQSRLRKGK 367
           KPR+I+KVAV+GGGLMGSGIATAL++SN  V+LKEVN +FL+ G   + ANL+  +++G 
Sbjct: 306 KPRKIRKVAVIGGGLMGSGIATALLVSNTSVVLKEVNPQFLQRGQKMIAANLEGLVKRGS 365

Query: 368 MTPEKFEKTISLLDGALDYESFKDVDMVIEAVIENVSLKQQIFVDLEKYCPPHCILATNT 427
           +T +K  K +SLL GALDY  FKDVDMVIEAVIE + LKQ IF DLEK CPPHCILATNT
Sbjct: 366 LTKDKMNKAMSLLKGALDYSDFKDVDMVIEAVIEKIPLKQSIFSDLEKVCPPHCILATNT 425

Query: 428 STIDLELIGERTKSHDRIVGAHFFSPAHVMPLLEVVRTKRTAPQVIVDLIDVGKKIKKAP 487
           STIDL ++GE+T S DRI+GAHFFSPAH+MPLLE+VRT++T+PQ I+DLI VGK IKK P
Sbjct: 426 STIDLNVVGEKTNSQDRIIGAHFFSPAHIMPLLEIVRTEKTSPQAILDLITVGKMIKKVP 485

Query: 488 VVVGNCTGFAVNRMFFPYTQAALLLVEHGVDPYQMDRAVFKFGMPMGPFRLIDLVGFGVA 547
           VVVGNCTGFAVNR FFPYTQ + LLV  G+D +++DR +  FGMPMGPF+L DL G+GVA
Sbjct: 486 VVVGNCTGFAVNRTFFPYTQGSHLLVSIGIDVFRIDRVISSFGMPMGPFQLQDLAGYGVA 545

Query: 548 IATGGQFVQNFPDRTFKSMIIPLMQEDKRAGETTQKGFYIYDKNRKAKPDPELKKYIEKA 607
           +A    +   F  R   S ++ LM ++ R G++  KG+Y+Y+K  K KPDP ++  I++ 
Sbjct: 546 LAVKDIYAAAFGTRNLDSNLVDLMVQNGRQGKSNGKGYYLYEKGGKPKPDPSVQVVIDEY 605

Query: 608 RTMSGISIDHKLTKLTEKDIIEMVFFPVVNEACRVLAEGIAVKAADLDIAGVMGMGFPPY 667
           R  +      K   L+++DI+EM+FFPVVNEACRV+ E + ++A+DLDIA ++GMGFP +
Sbjct: 606 RRCAKTMPGGKPVTLSDQDILEMIFFPVVNEACRVMDENVVIRASDLDIASILGMGFPKF 665

Query: 668 RGGVMFWADSLGSKYIYSRLEEWSKHYGGFFKPCAYLAERAAQGATL 715
           RGG++FWAD++G+ YI+S+L +W++ YG FFKP +YL +RA +   L
Sbjct: 666 RGGLVFWADTIGAPYIHSKLSKWTEIYGDFFKPSSYLEDRAKRSLPL 711

BLAST of CmaCh08G012320 vs. ExPASy TrEMBL
Match: A0A6J1KLC6 (3-hydroxyacyl-CoA dehydrogenase OS=Cucurbita maxima OX=3661 GN=LOC111494319 PE=3 SV=1)

HSP 1 Score: 1407.1 bits (3641), Expect = 0.0e+00
Identity = 715/716 (99.86%), Postives = 715/716 (99.86%), Query Frame = 0

Query: 1   MGSQAKGRTTLEVGADGIALITIINPPVNSLSFDVLFSLKESYEQALQREDVKAIVLTGA 60
           MGSQAKGRTTLEVGADGIALITIINPPVNSLSFDVLFSLKESYEQALQREDVKAIVLTGA
Sbjct: 1   MGSQAKGRTTLEVGADGIALITIINPPVNSLSFDVLFSLKESYEQALQREDVKAIVLTGA 60

Query: 61  RGKFSGGFDITAFGGLQGGKAREPRPGYISVDVITDIFEAARKPAVAAIDGLALGGGLEV 120
           RGKFSGGFDITAFGGLQGGKAREPRPGYISVDVITDIFEAARKPAVAAIDGLALGGGLEV
Sbjct: 61  RGKFSGGFDITAFGGLQGGKAREPRPGYISVDVITDIFEAARKPAVAAIDGLALGGGLEV 120

Query: 121 AMACHARVSTKTTLLGLPELQLGLIPGFGGTQRLPRLVGLSKALEMMLTSMPVKGEEAFS 180
           AMACHARVSTKTTLLGLPELQLGLIPGFGGTQRLPRLVGLSKALEMMLTSMPVKGEEAFS
Sbjct: 121 AMACHARVSTKTTLLGLPELQLGLIPGFGGTQRLPRLVGLSKALEMMLTSMPVKGEEAFS 180

Query: 181 LGLVDAIVPSEELISTARKLALDISERRKPWIISLHRTDKLESLGDAREIFKFARAQIRK 240
           LGLVDAIVPSEELISTARKLALDISERRKPWIISLHRTDKLESLGDAREIFKFARAQIRK
Sbjct: 181 LGLVDAIVPSEELISTARKLALDISERRKPWIISLHRTDKLESLGDAREIFKFARAQIRK 240

Query: 241 QAPNLMHPLVCIDVVETGVVSGPRAGLWKEIEDFQVLLHADTSKSLINIFFAQRGTTKVP 300
           QAPNLMHPLVCIDVVETGVVSGPRAGLWKEIEDFQVLLHADTSKSLINIFFAQRGTTKVP
Sbjct: 241 QAPNLMHPLVCIDVVETGVVSGPRAGLWKEIEDFQVLLHADTSKSLINIFFAQRGTTKVP 300

Query: 301 GVTDLGLKPRRIKKVAVVGGGLMGSGIATALVLSNYPVILKEVNDKFLKAGLDRVRANLQ 360
           GVTDLGLKPRRIKKVAVVGGGLMGSGIATALVLSNYPVILKEVNDKFLKAGLDRVRANLQ
Sbjct: 301 GVTDLGLKPRRIKKVAVVGGGLMGSGIATALVLSNYPVILKEVNDKFLKAGLDRVRANLQ 360

Query: 361 SRLRKGKMTPEKFEKTISLLDGALDYESFKDVDMVIEAVIENVSLKQQIFVDLEKYCPPH 420
           SRLRKGKMTPEKFEKTISLLDGALDYESFKDVDMVIEAVIENVSLKQQIFVDLEKYCPPH
Sbjct: 361 SRLRKGKMTPEKFEKTISLLDGALDYESFKDVDMVIEAVIENVSLKQQIFVDLEKYCPPH 420

Query: 421 CILATNTSTIDLELIGERTKSHDRIVGAHFFSPAHVMPLLEVVRTKRTAPQVIVDLIDVG 480
           CILATNTSTIDLELIGERTKSHDRIVGAHFFSPAHVMPLLEVVRTKRTAPQVIVDLIDVG
Sbjct: 421 CILATNTSTIDLELIGERTKSHDRIVGAHFFSPAHVMPLLEVVRTKRTAPQVIVDLIDVG 480

Query: 481 KKIKKAPVVVGNCTGFAVNRMFFPYTQAALLLVEHGVDPYQMDRAVFKFGMPMGPFRLID 540
           KKIKKAPVVVGNCTGFAVNRMFFPYTQAALLLVEHGVDPYQMDRAVFKFGMPMGPFRLID
Sbjct: 481 KKIKKAPVVVGNCTGFAVNRMFFPYTQAALLLVEHGVDPYQMDRAVFKFGMPMGPFRLID 540

Query: 541 LVGFGVAIATGGQFVQNFPDRTFKSMIIPLMQEDKRAGETTQKGFYIYDKNRKAKPDPEL 600
           LVGFGVAIATGGQFVQNFPDRTFKSMIIPLMQEDKRAGETTQKGFYIYDKNRKAKPDPEL
Sbjct: 541 LVGFGVAIATGGQFVQNFPDRTFKSMIIPLMQEDKRAGETTQKGFYIYDKNRKAKPDPEL 600

Query: 601 KKYIEKARTMSGISIDHKLTKLTEKDIIEMVFFPVVNEACRVLAEGIAVKAADLDIAGVM 660
           KKYIEKARTMSGISIDHKLTKLTEKDIIEMVFFPVVNEACRVLAEGIAVKAADLDIAGVM
Sbjct: 601 KKYIEKARTMSGISIDHKLTKLTEKDIIEMVFFPVVNEACRVLAEGIAVKAADLDIAGVM 660

Query: 661 GMGFPPYRGGVMFWADSLGSKYIYSRLEEWSKHYGGFFKPCAYLAERAAQGATLVS 717
           GMGFPPYRGGVMFWADSLGSKYIYSRLEEWSKHYGGFFKPCAYLAERAAQGATL S
Sbjct: 661 GMGFPPYRGGVMFWADSLGSKYIYSRLEEWSKHYGGFFKPCAYLAERAAQGATLSS 716

BLAST of CmaCh08G012320 vs. ExPASy TrEMBL
Match: A0A6J1EWF3 (3-hydroxyacyl-CoA dehydrogenase OS=Cucurbita moschata OX=3662 GN=LOC111436753 PE=3 SV=1)

HSP 1 Score: 1381.7 bits (3575), Expect = 0.0e+00
Identity = 704/716 (98.32%), Postives = 707/716 (98.74%), Query Frame = 0

Query: 1   MGSQAKGRTTLEVGADGIALITIINPPVNSLSFDVLFSLKESYEQALQREDVKAIVLTGA 60
           MGSQAKGRTTLEVGADGIALITIINPPVNSLSFDVLFSLKESYEQALQREDVKAIVLTGA
Sbjct: 1   MGSQAKGRTTLEVGADGIALITIINPPVNSLSFDVLFSLKESYEQALQREDVKAIVLTGA 60

Query: 61  RGKFSGGFDITAFGGLQGGKAREPRPGYISVDVITDIFEAARKPAVAAIDGLALGGGLEV 120
           RGKFSGGFDITAFGGLQGGKAREPRPGYISVDVITDIFEAARKPAVAAIDGLALGGGLEV
Sbjct: 61  RGKFSGGFDITAFGGLQGGKAREPRPGYISVDVITDIFEAARKPAVAAIDGLALGGGLEV 120

Query: 121 AMACHARVSTKTTLLGLPELQLGLIPGFGGTQRLPRLVGLSKALEMMLTSMPVKGEEAFS 180
           AMACHARVSTKT LLGLPELQLGLIPGFGGTQRLPRLVGLSKALEMMLTS PVKGEEAFS
Sbjct: 121 AMACHARVSTKTALLGLPELQLGLIPGFGGTQRLPRLVGLSKALEMMLTSKPVKGEEAFS 180

Query: 181 LGLVDAIVPSEELISTARKLALDISERRKPWIISLHRTDKLESLGDAREIFKFARAQIRK 240
           LGLVDAIVPSEELISTARKLALDISERRKPWIISLHRTDKLESLGDAREIFKFARAQIRK
Sbjct: 181 LGLVDAIVPSEELISTARKLALDISERRKPWIISLHRTDKLESLGDAREIFKFARAQIRK 240

Query: 241 QAPNLMHPLVCIDVVETGVVSGPRAGLWKEIEDFQVLLHADTSKSLINIFFAQRGTTKVP 300
           QAPNL HPLVCIDVVETGVVSGPRAGLWKEIEDFQVLLHADTSKSLINIFFAQRGTTKVP
Sbjct: 241 QAPNLTHPLVCIDVVETGVVSGPRAGLWKEIEDFQVLLHADTSKSLINIFFAQRGTTKVP 300

Query: 301 GVTDLGLKPRRIKKVAVVGGGLMGSGIATALVLSNYPVILKEVNDKFLKAGLDRVRANLQ 360
           GVTDLGLKPRRIKKVAVVGGGLMGSGIATALVLSNYPVILKEVNDKFLKAGLDRVRANLQ
Sbjct: 301 GVTDLGLKPRRIKKVAVVGGGLMGSGIATALVLSNYPVILKEVNDKFLKAGLDRVRANLQ 360

Query: 361 SRLRKGKMTPEKFEKTISLLDGALDYESFKDVDMVIEAVIENVSLKQQIFVDLEKYCPPH 420
           S+LRKGKMTPEKFEKTISLL GALDYESFKDVDMVIEAVIENVSLKQQIFVDLEKYCPPH
Sbjct: 361 SQLRKGKMTPEKFEKTISLLHGALDYESFKDVDMVIEAVIENVSLKQQIFVDLEKYCPPH 420

Query: 421 CILATNTSTIDLELIGERTKSHDRIVGAHFFSPAHVMPLLEVVRTKRTAPQVIVDLIDVG 480
           CILATNTSTIDLELIGERTKSHDRIVGAHFFSPAHVMPLLEVVRTKRTAPQVIVDLIDVG
Sbjct: 421 CILATNTSTIDLELIGERTKSHDRIVGAHFFSPAHVMPLLEVVRTKRTAPQVIVDLIDVG 480

Query: 481 KKIKKAPVVVGNCTGFAVNRMFFPYTQAALLLVEHGVDPYQMDRAVFKFGMPMGPFRLID 540
           KKIKK PVVVGNCTGFAVNRMFFPYTQAALLLVEHGVDPYQ+DRAV KFGMPMGPFRLID
Sbjct: 481 KKIKKTPVVVGNCTGFAVNRMFFPYTQAALLLVEHGVDPYQIDRAVSKFGMPMGPFRLID 540

Query: 541 LVGFGVAIATGGQFVQNFPDRTFKSMIIPLMQEDKRAGETTQKGFYIYDKNRKAKPDPEL 600
           LVGFGVAIATGGQFVQNFPDRTFKSMIIPLMQEDKRAGETTQKGFYIYDKNRKAKPDPEL
Sbjct: 541 LVGFGVAIATGGQFVQNFPDRTFKSMIIPLMQEDKRAGETTQKGFYIYDKNRKAKPDPEL 600

Query: 601 KKYIEKARTMSGISIDHKLTKLTEKDIIEMVFFPVVNEACRVLAEGIAVKAADLDIAGVM 660
           KKYIEKAR+MSGISID KLTKLTEKDIIEMVFFPVVNEACRVLAEGIAVKAADLDIAGVM
Sbjct: 601 KKYIEKARSMSGISIDPKLTKLTEKDIIEMVFFPVVNEACRVLAEGIAVKAADLDIAGVM 660

Query: 661 GMGFPPYRGGVMFWADSLGSKYIYSRLEEWSKHYGGFFKPCAYLAERAAQGATLVS 717
           GMGFPPYRGGVMFWADSLGSKYIYSRLEEWSK YGGFFKPCAYLAERAAQGATL S
Sbjct: 661 GMGFPPYRGGVMFWADSLGSKYIYSRLEEWSKRYGGFFKPCAYLAERAAQGATLSS 716

BLAST of CmaCh08G012320 vs. ExPASy TrEMBL
Match: A0A6J1CBF0 (3-hydroxyacyl-CoA dehydrogenase OS=Momordica charantia OX=3673 GN=LOC111009683 PE=3 SV=1)

HSP 1 Score: 1310.4 bits (3390), Expect = 0.0e+00
Identity = 657/716 (91.76%), Postives = 691/716 (96.51%), Query Frame = 0

Query: 1   MGSQAKGRTTLEVGADGIALITIINPPVNSLSFDVLFSLKESYEQALQREDVKAIVLTGA 60
           MGS+AKGRTTLEVGADGI LITIINPPVNSLSFDVLFSLK+SYEQALQREDVKAIV+TGA
Sbjct: 1   MGSKAKGRTTLEVGADGIGLITIINPPVNSLSFDVLFSLKDSYEQALQREDVKAIVITGA 60

Query: 61  RGKFSGGFDITAFGGLQGGKAREPRPGYISVDVITDIFEAARKPAVAAIDGLALGGGLEV 120
           RGKFSGGFDI+AFGGLQGGKA EPRPGYISVDVITDIFEAARKPAVAAIDGLALGGGLEV
Sbjct: 61  RGKFSGGFDISAFGGLQGGKAEEPRPGYISVDVITDIFEAARKPAVAAIDGLALGGGLEV 120

Query: 121 AMACHARVSTKTTLLGLPELQLGLIPGFGGTQRLPRLVGLSKALEMMLTSMPVKGEEAFS 180
           AMACHAR+STKT  LGLPELQLG+IPGFGGTQRLPRLVGLSKALEMMLTS PVKGEEAFS
Sbjct: 121 AMACHARISTKTAQLGLPELQLGIIPGFGGTQRLPRLVGLSKALEMMLTSKPVKGEEAFS 180

Query: 181 LGLVDAIVPSEELISTARKLALDISERRKPWIISLHRTDKLESLGDAREIFKFARAQIRK 240
           LGLVDAIVPSEELISTARK ALDISERRKPWI+SL++TDKLESLGDAREIFKFARAQIRK
Sbjct: 181 LGLVDAIVPSEELISTARKWALDISERRKPWILSLYKTDKLESLGDAREIFKFARAQIRK 240

Query: 241 QAPNLMHPLVCIDVVETGVVSGPRAGLWKEIEDFQVLLHADTSKSLINIFFAQRGTTKVP 300
           QAPNL HPLVCIDVVE GVVSGPR GLWKEIEDFQ+LLH+DTSKSLI+IFFAQRGTTKVP
Sbjct: 241 QAPNLKHPLVCIDVVERGVVSGPRDGLWKEIEDFQILLHSDTSKSLIHIFFAQRGTTKVP 300

Query: 301 GVTDLGLKPRRIKKVAVVGGGLMGSGIATALVLSNYPVILKEVNDKFLKAGLDRVRANLQ 360
           GVTDLGL PRRI KVAVVGGGLMGSGIATAL+LSNYPVILKEVN+KFL+AG+ RV+ANLQ
Sbjct: 301 GVTDLGLTPRRINKVAVVGGGLMGSGIATALILSNYPVILKEVNEKFLEAGIGRVKANLQ 360

Query: 361 SRLRKGKMTPEKFEKTISLLDGALDYESFKDVDMVIEAVIENVSLKQQIFVDLEKYCPPH 420
           SR+RKGKMTPEKFEK ISLL GALDYESFKDVD+VIEAVIEN+SLKQQIFVDLEKYCPPH
Sbjct: 361 SRVRKGKMTPEKFEKAISLLKGALDYESFKDVDIVIEAVIENISLKQQIFVDLEKYCPPH 420

Query: 421 CILATNTSTIDLELIGERTKSHDRIVGAHFFSPAHVMPLLEVVRTKRTAPQVIVDLIDVG 480
           CILATNTSTIDLELIGE+ KS+DRIVGAHFFSPAHVMPLLEVVRTK T+ QV+VDL+DVG
Sbjct: 421 CILATNTSTIDLELIGEKIKSNDRIVGAHFFSPAHVMPLLEVVRTKGTSTQVVVDLLDVG 480

Query: 481 KKIKKAPVVVGNCTGFAVNRMFFPYTQAALLLVEHGVDPYQMDRAVFKFGMPMGPFRLID 540
           KK+KK PVVVGNCTGFAVNRMFFPYTQA+LLLV+HGVDPYQ+D+A+ KFGMPMGPFRLID
Sbjct: 481 KKMKKTPVVVGNCTGFAVNRMFFPYTQASLLLVQHGVDPYQLDKAISKFGMPMGPFRLID 540

Query: 541 LVGFGVAIATGGQFVQNFPDRTFKSMIIPLMQEDKRAGETTQKGFYIYDKNRKAKPDPEL 600
           LVGFGVAIATGGQFVQNFPDRTFKSMIIPLMQEDKRAGETTQKGFYIYDKNRKAKPDPE+
Sbjct: 541 LVGFGVAIATGGQFVQNFPDRTFKSMIIPLMQEDKRAGETTQKGFYIYDKNRKAKPDPEI 600

Query: 601 KKYIEKARTMSGISIDHKLTKLTEKDIIEMVFFPVVNEACRVLAEGIAVKAADLDIAGVM 660
            KYIEKAR++SG SID KLTKL EKDIIEMVFFPVVNEACRVLAEGIAVKAADLDIAGVM
Sbjct: 601 YKYIEKARSLSGTSIDPKLTKLPEKDIIEMVFFPVVNEACRVLAEGIAVKAADLDIAGVM 660

Query: 661 GMGFPPYRGGVMFWADSLGSKYIYSRLEEWSKHYGGFFKPCAYLAERAAQGATLVS 717
           GMGFPPYRGGVMFWADSLGSKYIYSRLEEWSK YGGFFKPCAYLAERAAQGATL S
Sbjct: 661 GMGFPPYRGGVMFWADSLGSKYIYSRLEEWSKLYGGFFKPCAYLAERAAQGATLSS 716

BLAST of CmaCh08G012320 vs. ExPASy TrEMBL
Match: A0A0A0KI31 (3-hydroxyacyl-CoA dehydrogenase OS=Cucumis sativus OX=3659 GN=Csa_6G319770 PE=3 SV=1)

HSP 1 Score: 1304.3 bits (3374), Expect = 0.0e+00
Identity = 650/716 (90.78%), Postives = 688/716 (96.09%), Query Frame = 0

Query: 1   MGSQAKGRTTLEVGADGIALITIINPPVNSLSFDVLFSLKESYEQALQREDVKAIVLTGA 60
           M ++ KGRTTLEVGADG+ALITIINPPVNSLSFDVLFSLKESYEQAL+REDVKAIV+TGA
Sbjct: 1   MATKVKGRTTLEVGADGVALITIINPPVNSLSFDVLFSLKESYEQALEREDVKAIVVTGA 60

Query: 61  RGKFSGGFDITAFGGLQGGKAREPRPGYISVDVITDIFEAARKPAVAAIDGLALGGGLEV 120
           RGKFSGGFDITAFGGLQGGKA EPRPGYIS+DVITDIFEAARKPAVAAIDGLALGGGLEV
Sbjct: 61  RGKFSGGFDITAFGGLQGGKAEEPRPGYISIDVITDIFEAARKPAVAAIDGLALGGGLEV 120

Query: 121 AMACHARVSTKTTLLGLPELQLGLIPGFGGTQRLPRLVGLSKALEMMLTSMPVKGEEAFS 180
           AMACHAR+STKT  LGLPELQLGLIPGFGGTQRLPRLVGL KALEMMLTS PVKGEEAFS
Sbjct: 121 AMACHARLSTKTAQLGLPELQLGLIPGFGGTQRLPRLVGLPKALEMMLTSKPVKGEEAFS 180

Query: 181 LGLVDAIVPSEELISTARKLALDISERRKPWIISLHRTDKLESLGDAREIFKFARAQIRK 240
           LGLVDAIVP EELIS ARK ALDISERRKPWIISLH+T+KLESL DAREIFKFARAQ+RK
Sbjct: 181 LGLVDAIVPREELISAARKWALDISERRKPWIISLHKTNKLESLADAREIFKFARAQVRK 240

Query: 241 QAPNLMHPLVCIDVVETGVVSGPRAGLWKEIEDFQVLLHADTSKSLINIFFAQRGTTKVP 300
           QAPNL HPLVCIDVVETGVVSGPRAGL KEIEDFQVLLHADTSKSLI++FFAQRGTTKVP
Sbjct: 241 QAPNLKHPLVCIDVVETGVVSGPRAGLQKEIEDFQVLLHADTSKSLIHVFFAQRGTTKVP 300

Query: 301 GVTDLGLKPRRIKKVAVVGGGLMGSGIATALVLSNYPVILKEVNDKFLKAGLDRVRANLQ 360
           GV+DLGL PRRI KVAV+GGGLMGSGIATAL+LSNYPVILKEVN+KFL+AGL RV+ANLQ
Sbjct: 301 GVSDLGLTPRRINKVAVIGGGLMGSGIATALILSNYPVILKEVNEKFLEAGLGRVKANLQ 360

Query: 361 SRLRKGKMTPEKFEKTISLLDGALDYESFKDVDMVIEAVIENVSLKQQIFVDLEKYCPPH 420
           SR+RKG MTPEKFE+TISLL G LDYESFKDVDMVIEAVIEN+SLKQQI VDLEKYCPPH
Sbjct: 361 SRVRKGTMTPEKFERTISLLKGVLDYESFKDVDMVIEAVIENISLKQQIIVDLEKYCPPH 420

Query: 421 CILATNTSTIDLELIGERTKSHDRIVGAHFFSPAHVMPLLEVVRTKRTAPQVIVDLIDVG 480
           CILATNTSTIDL+LIGE+T SHDRIVGAHFFSPAHVMPLLEVVRT RTAPQVIVDL+DVG
Sbjct: 421 CILATNTSTIDLDLIGEKTNSHDRIVGAHFFSPAHVMPLLEVVRTNRTAPQVIVDLVDVG 480

Query: 481 KKIKKAPVVVGNCTGFAVNRMFFPYTQAALLLVEHGVDPYQMDRAVFKFGMPMGPFRLID 540
           K+IKK PVVVGNCTGFAVNR+FFPYTQAALLLVEHGVDPYQ+DRA++KFGMPMGPFRLID
Sbjct: 481 KRIKKTPVVVGNCTGFAVNRIFFPYTQAALLLVEHGVDPYQIDRAIYKFGMPMGPFRLID 540

Query: 541 LVGFGVAIATGGQFVQNFPDRTFKSMIIPLMQEDKRAGETTQKGFYIYDKNRKAKPDPEL 600
           LVGFGVAIATGGQFVQNFPDRTFKSMIIPLMQEDKRAGETTQKGFY+YDK RK+KPDPEL
Sbjct: 541 LVGFGVAIATGGQFVQNFPDRTFKSMIIPLMQEDKRAGETTQKGFYLYDKKRKSKPDPEL 600

Query: 601 KKYIEKARTMSGISIDHKLTKLTEKDIIEMVFFPVVNEACRVLAEGIAVKAADLDIAGVM 660
           KKYIEKAR+MSGIS+D KL K+++KDIIEM+FFPVVNEACRVLAEGIAVKAADLDIAGVM
Sbjct: 601 KKYIEKARSMSGISVDPKLAKISDKDIIEMIFFPVVNEACRVLAEGIAVKAADLDIAGVM 660

Query: 661 GMGFPPYRGGVMFWADSLGSKYIYSRLEEWSKHYGGFFKPCAYLAERAAQGATLVS 717
           GMGFPPYRGGVMFWADSLGSKYIYSRLEEWSK YGGFFKPCAYLAERAAQG+TL S
Sbjct: 661 GMGFPPYRGGVMFWADSLGSKYIYSRLEEWSKLYGGFFKPCAYLAERAAQGSTLSS 716

BLAST of CmaCh08G012320 vs. ExPASy TrEMBL
Match: A0A1S3C8F0 (3-hydroxyacyl-CoA dehydrogenase OS=Cucumis melo OX=3656 GN=LOC103497841 PE=3 SV=1)

HSP 1 Score: 1303.9 bits (3373), Expect = 0.0e+00
Identity = 651/716 (90.92%), Postives = 688/716 (96.09%), Query Frame = 0

Query: 1   MGSQAKGRTTLEVGADGIALITIINPPVNSLSFDVLFSLKESYEQALQREDVKAIVLTGA 60
           M +Q KGRTTLEVGADG+ALITIINPPVNSLSFDVLFSLKESYEQAL REDVKAIV+TGA
Sbjct: 1   MATQVKGRTTLEVGADGVALITIINPPVNSLSFDVLFSLKESYEQALLREDVKAIVVTGA 60

Query: 61  RGKFSGGFDITAFGGLQGGKAREPRPGYISVDVITDIFEAARKPAVAAIDGLALGGGLEV 120
           RGKFSGGFDITAFGGLQGGKA EPRPGYIS+DVITDIFEAARKPAVAAIDGLALGGGLEV
Sbjct: 61  RGKFSGGFDITAFGGLQGGKAVEPRPGYISIDVITDIFEAARKPAVAAIDGLALGGGLEV 120

Query: 121 AMACHARVSTKTTLLGLPELQLGLIPGFGGTQRLPRLVGLSKALEMMLTSMPVKGEEAFS 180
           AMACHAR+STKT  LGLPELQLGLIPGFGGTQRLPRLVGL KALEMMLTS PVKGEEAFS
Sbjct: 121 AMACHARLSTKTAQLGLPELQLGLIPGFGGTQRLPRLVGLPKALEMMLTSKPVKGEEAFS 180

Query: 181 LGLVDAIVPSEELISTARKLALDISERRKPWIISLHRTDKLESLGDAREIFKFARAQIRK 240
           LGLVDAI+P EELIS ARK ALDISERRKPWIISLH+TDKLESL DAREIFKFARAQ+RK
Sbjct: 181 LGLVDAIIPPEELISAARKWALDISERRKPWIISLHKTDKLESLADAREIFKFARAQVRK 240

Query: 241 QAPNLMHPLVCIDVVETGVVSGPRAGLWKEIEDFQVLLHADTSKSLINIFFAQRGTTKVP 300
           QAPNL HPLVC+DVVETGVVSGPRAGL KEIEDFQVLLHADTSKSLI+IFFAQRGTTKVP
Sbjct: 241 QAPNLKHPLVCLDVVETGVVSGPRAGLRKEIEDFQVLLHADTSKSLIHIFFAQRGTTKVP 300

Query: 301 GVTDLGLKPRRIKKVAVVGGGLMGSGIATALVLSNYPVILKEVNDKFLKAGLDRVRANLQ 360
           GV+DLGL PRRI KVAV+GGGLMGSGIAT L+LSNY VILKEVN+KFL+AGL RV+ANLQ
Sbjct: 301 GVSDLGLTPRRINKVAVIGGGLMGSGIATTLILSNYSVILKEVNEKFLEAGLGRVKANLQ 360

Query: 361 SRLRKGKMTPEKFEKTISLLDGALDYESFKDVDMVIEAVIENVSLKQQIFVDLEKYCPPH 420
           S++RKGKMTPEKFE+TISLL G LDYESFKDVDMVIEAVIEN+SLKQQIFVDLEKYCPPH
Sbjct: 361 SQVRKGKMTPEKFERTISLLKGVLDYESFKDVDMVIEAVIENISLKQQIFVDLEKYCPPH 420

Query: 421 CILATNTSTIDLELIGERTKSHDRIVGAHFFSPAHVMPLLEVVRTKRTAPQVIVDLIDVG 480
           CILATNTSTIDL+LIGE+TKSHDRI+GAHFFSPAHVMPLLEVVRTKRTAPQVIVDL+DVG
Sbjct: 421 CILATNTSTIDLDLIGEKTKSHDRIIGAHFFSPAHVMPLLEVVRTKRTAPQVIVDLVDVG 480

Query: 481 KKIKKAPVVVGNCTGFAVNRMFFPYTQAALLLVEHGVDPYQMDRAVFKFGMPMGPFRLID 540
           K+IKK PVVVGNCTGFAVNRMFFPYTQAALLLVEHGVDPY +DRA+ KFGMPMGPFRLID
Sbjct: 481 KRIKKTPVVVGNCTGFAVNRMFFPYTQAALLLVEHGVDPYLIDRAISKFGMPMGPFRLID 540

Query: 541 LVGFGVAIATGGQFVQNFPDRTFKSMIIPLMQEDKRAGETTQKGFYIYDKNRKAKPDPEL 600
           LVGFGVAIATGGQFVQNFPDRTFKSMIIPLMQEDKRAGETTQKGFY+YDKNRK+KPDPEL
Sbjct: 541 LVGFGVAIATGGQFVQNFPDRTFKSMIIPLMQEDKRAGETTQKGFYLYDKNRKSKPDPEL 600

Query: 601 KKYIEKARTMSGISIDHKLTKLTEKDIIEMVFFPVVNEACRVLAEGIAVKAADLDIAGVM 660
           KKYIEKAR+MSGIS+D KL KL++KD+IEM+FFPVVNEACRVLAEGIAVKAADLDIAGVM
Sbjct: 601 KKYIEKARSMSGISVDPKLAKLSDKDLIEMIFFPVVNEACRVLAEGIAVKAADLDIAGVM 660

Query: 661 GMGFPPYRGGVMFWADSLGSKYIYSRLEEWSKHYGGFFKPCAYLAERAAQGATLVS 717
           GMGFPPYRGGVMFWADSLGSKYIYSRLEEWSK YGGFFKPC+YLAERAAQG+TL S
Sbjct: 661 GMGFPPYRGGVMFWADSLGSKYIYSRLEEWSKLYGGFFKPCSYLAERAAQGSTLSS 716

BLAST of CmaCh08G012320 vs. NCBI nr
Match: XP_023000007.1 (glyoxysomal fatty acid beta-oxidation multifunctional protein MFP-a-like [Cucurbita maxima] >XP_023000008.1 glyoxysomal fatty acid beta-oxidation multifunctional protein MFP-a-like [Cucurbita maxima])

HSP 1 Score: 1407.1 bits (3641), Expect = 0.0e+00
Identity = 715/716 (99.86%), Postives = 715/716 (99.86%), Query Frame = 0

Query: 1   MGSQAKGRTTLEVGADGIALITIINPPVNSLSFDVLFSLKESYEQALQREDVKAIVLTGA 60
           MGSQAKGRTTLEVGADGIALITIINPPVNSLSFDVLFSLKESYEQALQREDVKAIVLTGA
Sbjct: 1   MGSQAKGRTTLEVGADGIALITIINPPVNSLSFDVLFSLKESYEQALQREDVKAIVLTGA 60

Query: 61  RGKFSGGFDITAFGGLQGGKAREPRPGYISVDVITDIFEAARKPAVAAIDGLALGGGLEV 120
           RGKFSGGFDITAFGGLQGGKAREPRPGYISVDVITDIFEAARKPAVAAIDGLALGGGLEV
Sbjct: 61  RGKFSGGFDITAFGGLQGGKAREPRPGYISVDVITDIFEAARKPAVAAIDGLALGGGLEV 120

Query: 121 AMACHARVSTKTTLLGLPELQLGLIPGFGGTQRLPRLVGLSKALEMMLTSMPVKGEEAFS 180
           AMACHARVSTKTTLLGLPELQLGLIPGFGGTQRLPRLVGLSKALEMMLTSMPVKGEEAFS
Sbjct: 121 AMACHARVSTKTTLLGLPELQLGLIPGFGGTQRLPRLVGLSKALEMMLTSMPVKGEEAFS 180

Query: 181 LGLVDAIVPSEELISTARKLALDISERRKPWIISLHRTDKLESLGDAREIFKFARAQIRK 240
           LGLVDAIVPSEELISTARKLALDISERRKPWIISLHRTDKLESLGDAREIFKFARAQIRK
Sbjct: 181 LGLVDAIVPSEELISTARKLALDISERRKPWIISLHRTDKLESLGDAREIFKFARAQIRK 240

Query: 241 QAPNLMHPLVCIDVVETGVVSGPRAGLWKEIEDFQVLLHADTSKSLINIFFAQRGTTKVP 300
           QAPNLMHPLVCIDVVETGVVSGPRAGLWKEIEDFQVLLHADTSKSLINIFFAQRGTTKVP
Sbjct: 241 QAPNLMHPLVCIDVVETGVVSGPRAGLWKEIEDFQVLLHADTSKSLINIFFAQRGTTKVP 300

Query: 301 GVTDLGLKPRRIKKVAVVGGGLMGSGIATALVLSNYPVILKEVNDKFLKAGLDRVRANLQ 360
           GVTDLGLKPRRIKKVAVVGGGLMGSGIATALVLSNYPVILKEVNDKFLKAGLDRVRANLQ
Sbjct: 301 GVTDLGLKPRRIKKVAVVGGGLMGSGIATALVLSNYPVILKEVNDKFLKAGLDRVRANLQ 360

Query: 361 SRLRKGKMTPEKFEKTISLLDGALDYESFKDVDMVIEAVIENVSLKQQIFVDLEKYCPPH 420
           SRLRKGKMTPEKFEKTISLLDGALDYESFKDVDMVIEAVIENVSLKQQIFVDLEKYCPPH
Sbjct: 361 SRLRKGKMTPEKFEKTISLLDGALDYESFKDVDMVIEAVIENVSLKQQIFVDLEKYCPPH 420

Query: 421 CILATNTSTIDLELIGERTKSHDRIVGAHFFSPAHVMPLLEVVRTKRTAPQVIVDLIDVG 480
           CILATNTSTIDLELIGERTKSHDRIVGAHFFSPAHVMPLLEVVRTKRTAPQVIVDLIDVG
Sbjct: 421 CILATNTSTIDLELIGERTKSHDRIVGAHFFSPAHVMPLLEVVRTKRTAPQVIVDLIDVG 480

Query: 481 KKIKKAPVVVGNCTGFAVNRMFFPYTQAALLLVEHGVDPYQMDRAVFKFGMPMGPFRLID 540
           KKIKKAPVVVGNCTGFAVNRMFFPYTQAALLLVEHGVDPYQMDRAVFKFGMPMGPFRLID
Sbjct: 481 KKIKKAPVVVGNCTGFAVNRMFFPYTQAALLLVEHGVDPYQMDRAVFKFGMPMGPFRLID 540

Query: 541 LVGFGVAIATGGQFVQNFPDRTFKSMIIPLMQEDKRAGETTQKGFYIYDKNRKAKPDPEL 600
           LVGFGVAIATGGQFVQNFPDRTFKSMIIPLMQEDKRAGETTQKGFYIYDKNRKAKPDPEL
Sbjct: 541 LVGFGVAIATGGQFVQNFPDRTFKSMIIPLMQEDKRAGETTQKGFYIYDKNRKAKPDPEL 600

Query: 601 KKYIEKARTMSGISIDHKLTKLTEKDIIEMVFFPVVNEACRVLAEGIAVKAADLDIAGVM 660
           KKYIEKARTMSGISIDHKLTKLTEKDIIEMVFFPVVNEACRVLAEGIAVKAADLDIAGVM
Sbjct: 601 KKYIEKARTMSGISIDHKLTKLTEKDIIEMVFFPVVNEACRVLAEGIAVKAADLDIAGVM 660

Query: 661 GMGFPPYRGGVMFWADSLGSKYIYSRLEEWSKHYGGFFKPCAYLAERAAQGATLVS 717
           GMGFPPYRGGVMFWADSLGSKYIYSRLEEWSKHYGGFFKPCAYLAERAAQGATL S
Sbjct: 661 GMGFPPYRGGVMFWADSLGSKYIYSRLEEWSKHYGGFFKPCAYLAERAAQGATLSS 716

BLAST of CmaCh08G012320 vs. NCBI nr
Match: KAG6593976.1 (hypothetical protein SDJN03_13452, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1384.4 bits (3582), Expect = 0.0e+00
Identity = 705/716 (98.46%), Postives = 708/716 (98.88%), Query Frame = 0

Query: 1   MGSQAKGRTTLEVGADGIALITIINPPVNSLSFDVLFSLKESYEQALQREDVKAIVLTGA 60
           MGSQAKGRTTLEVGADGIALITIINPPVNSLSFDVLFSLKESYEQALQREDVKAIVLTGA
Sbjct: 1   MGSQAKGRTTLEVGADGIALITIINPPVNSLSFDVLFSLKESYEQALQREDVKAIVLTGA 60

Query: 61  RGKFSGGFDITAFGGLQGGKAREPRPGYISVDVITDIFEAARKPAVAAIDGLALGGGLEV 120
           RGKFSGGFDITAFGGLQGGKAREPRPGYISVDVITDIFEAARKPAVAAIDGLALGGGLEV
Sbjct: 61  RGKFSGGFDITAFGGLQGGKAREPRPGYISVDVITDIFEAARKPAVAAIDGLALGGGLEV 120

Query: 121 AMACHARVSTKTTLLGLPELQLGLIPGFGGTQRLPRLVGLSKALEMMLTSMPVKGEEAFS 180
           AMACHARVSTKT LLGLPELQLGLIPGFGGTQRLPRLVGLSKALEMMLTS PVKGEEAFS
Sbjct: 121 AMACHARVSTKTALLGLPELQLGLIPGFGGTQRLPRLVGLSKALEMMLTSKPVKGEEAFS 180

Query: 181 LGLVDAIVPSEELISTARKLALDISERRKPWIISLHRTDKLESLGDAREIFKFARAQIRK 240
           LGLVDAIVPSEELISTARKLALDISERRKPWIISLHRTDKLESLGDAREIFKFARAQIRK
Sbjct: 181 LGLVDAIVPSEELISTARKLALDISERRKPWIISLHRTDKLESLGDAREIFKFARAQIRK 240

Query: 241 QAPNLMHPLVCIDVVETGVVSGPRAGLWKEIEDFQVLLHADTSKSLINIFFAQRGTTKVP 300
           QAPNL HPLVCIDVVETGVVSGPRAGLWKEIEDFQVLLHADTSKSLINIFFAQRGTTKVP
Sbjct: 241 QAPNLTHPLVCIDVVETGVVSGPRAGLWKEIEDFQVLLHADTSKSLINIFFAQRGTTKVP 300

Query: 301 GVTDLGLKPRRIKKVAVVGGGLMGSGIATALVLSNYPVILKEVNDKFLKAGLDRVRANLQ 360
           GVTDLGLKPRRIKKVAVVGGGLMGSGIATALVLSNYPVILKEVNDKFLKAGLDRVRANLQ
Sbjct: 301 GVTDLGLKPRRIKKVAVVGGGLMGSGIATALVLSNYPVILKEVNDKFLKAGLDRVRANLQ 360

Query: 361 SRLRKGKMTPEKFEKTISLLDGALDYESFKDVDMVIEAVIENVSLKQQIFVDLEKYCPPH 420
           S+LRKGKMTPEKFEKTISLLDGALDYESFKDVDMVIEAVIENVSLKQQIFVDLEKYCPPH
Sbjct: 361 SQLRKGKMTPEKFEKTISLLDGALDYESFKDVDMVIEAVIENVSLKQQIFVDLEKYCPPH 420

Query: 421 CILATNTSTIDLELIGERTKSHDRIVGAHFFSPAHVMPLLEVVRTKRTAPQVIVDLIDVG 480
           CILATNTSTIDLELIGERTKSHDRIVGAHFFSPAHVMPLLEVVRTKRTAPQVIVDLIDVG
Sbjct: 421 CILATNTSTIDLELIGERTKSHDRIVGAHFFSPAHVMPLLEVVRTKRTAPQVIVDLIDVG 480

Query: 481 KKIKKAPVVVGNCTGFAVNRMFFPYTQAALLLVEHGVDPYQMDRAVFKFGMPMGPFRLID 540
           KKIKK PVVVGNCTGFAVNRMFFPYTQAALLLVEHGVDPYQ+DRAV KFGMPMGPFRLID
Sbjct: 481 KKIKKTPVVVGNCTGFAVNRMFFPYTQAALLLVEHGVDPYQIDRAVSKFGMPMGPFRLID 540

Query: 541 LVGFGVAIATGGQFVQNFPDRTFKSMIIPLMQEDKRAGETTQKGFYIYDKNRKAKPDPEL 600
           LVGFGVAIATGGQFVQNFPDRTFKSMIIPLMQEDKRAGETTQKGFYIYDKNRKAKPDPEL
Sbjct: 541 LVGFGVAIATGGQFVQNFPDRTFKSMIIPLMQEDKRAGETTQKGFYIYDKNRKAKPDPEL 600

Query: 601 KKYIEKARTMSGISIDHKLTKLTEKDIIEMVFFPVVNEACRVLAEGIAVKAADLDIAGVM 660
           KKYIEKAR+MSGISID KLTKLTEKDIIEMVFFPVVNEACRVLAEGIAVKAADLDIAGVM
Sbjct: 601 KKYIEKARSMSGISIDPKLTKLTEKDIIEMVFFPVVNEACRVLAEGIAVKAADLDIAGVM 660

Query: 661 GMGFPPYRGGVMFWADSLGSKYIYSRLEEWSKHYGGFFKPCAYLAERAAQGATLVS 717
           GMGFPPYRGGVMFWADSLGSKYIYSRLEEWSK YGGFFKPCAYLAERAAQGATL S
Sbjct: 661 GMGFPPYRGGVMFWADSLGSKYIYSRLEEWSKRYGGFFKPCAYLAERAAQGATLSS 716

BLAST of CmaCh08G012320 vs. NCBI nr
Match: KAG7026317.1 (hypothetical protein SDJN02_12818 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1384.4 bits (3582), Expect = 0.0e+00
Identity = 705/716 (98.46%), Postives = 708/716 (98.88%), Query Frame = 0

Query: 1   MGSQAKGRTTLEVGADGIALITIINPPVNSLSFDVLFSLKESYEQALQREDVKAIVLTGA 60
           MGSQAKGRTTLEVGADGIALITIINPPVNSLSFDVLFSLKESYEQALQREDVKAIVLTGA
Sbjct: 1   MGSQAKGRTTLEVGADGIALITIINPPVNSLSFDVLFSLKESYEQALQREDVKAIVLTGA 60

Query: 61  RGKFSGGFDITAFGGLQGGKAREPRPGYISVDVITDIFEAARKPAVAAIDGLALGGGLEV 120
           RGKFSGGFDITAFGGLQGGKAREPRPGYISVDVITDIFEAARKPAVAAIDGLALGGGLEV
Sbjct: 61  RGKFSGGFDITAFGGLQGGKAREPRPGYISVDVITDIFEAARKPAVAAIDGLALGGGLEV 120

Query: 121 AMACHARVSTKTTLLGLPELQLGLIPGFGGTQRLPRLVGLSKALEMMLTSMPVKGEEAFS 180
           AMACHARVSTKT LLGLPELQLGLIPGFGGTQRLPRLVGLSKALEMMLTS PVKGEEAFS
Sbjct: 121 AMACHARVSTKTALLGLPELQLGLIPGFGGTQRLPRLVGLSKALEMMLTSKPVKGEEAFS 180

Query: 181 LGLVDAIVPSEELISTARKLALDISERRKPWIISLHRTDKLESLGDAREIFKFARAQIRK 240
           LGLVDAIVPSEELISTARKLALDISERRKPWIISLHRTDKLESLGDAREIFKFARAQIRK
Sbjct: 181 LGLVDAIVPSEELISTARKLALDISERRKPWIISLHRTDKLESLGDAREIFKFARAQIRK 240

Query: 241 QAPNLMHPLVCIDVVETGVVSGPRAGLWKEIEDFQVLLHADTSKSLINIFFAQRGTTKVP 300
           QAPNL HPLVCIDVVETGVVSGPRAGLWKEIEDFQVLLHADTSKSLINIFFAQRGTTKVP
Sbjct: 241 QAPNLTHPLVCIDVVETGVVSGPRAGLWKEIEDFQVLLHADTSKSLINIFFAQRGTTKVP 300

Query: 301 GVTDLGLKPRRIKKVAVVGGGLMGSGIATALVLSNYPVILKEVNDKFLKAGLDRVRANLQ 360
           GVTDLGLKPRRIKKVAVVGGGLMGSGIATALVLSNYPVILKEVNDKFLKAGLDRVRANLQ
Sbjct: 301 GVTDLGLKPRRIKKVAVVGGGLMGSGIATALVLSNYPVILKEVNDKFLKAGLDRVRANLQ 360

Query: 361 SRLRKGKMTPEKFEKTISLLDGALDYESFKDVDMVIEAVIENVSLKQQIFVDLEKYCPPH 420
           S+LRKGKMTPEKFEKTISLLDGALDYESFKDVDMVIEAVIENVSLKQQIFVDLEKYCPPH
Sbjct: 361 SQLRKGKMTPEKFEKTISLLDGALDYESFKDVDMVIEAVIENVSLKQQIFVDLEKYCPPH 420

Query: 421 CILATNTSTIDLELIGERTKSHDRIVGAHFFSPAHVMPLLEVVRTKRTAPQVIVDLIDVG 480
           CILATNTSTIDLELIGERTKSHDRIVGAHFFSPAHVMPLLEVVRTKRTAPQVIVDLIDVG
Sbjct: 421 CILATNTSTIDLELIGERTKSHDRIVGAHFFSPAHVMPLLEVVRTKRTAPQVIVDLIDVG 480

Query: 481 KKIKKAPVVVGNCTGFAVNRMFFPYTQAALLLVEHGVDPYQMDRAVFKFGMPMGPFRLID 540
           KKIKK PVVVGNCTGFAVNRMFFPYTQAALLLVEHGVDPYQ+DRAV KFGMPMGPFRLID
Sbjct: 481 KKIKKTPVVVGNCTGFAVNRMFFPYTQAALLLVEHGVDPYQIDRAVSKFGMPMGPFRLID 540

Query: 541 LVGFGVAIATGGQFVQNFPDRTFKSMIIPLMQEDKRAGETTQKGFYIYDKNRKAKPDPEL 600
           LVGFGVAIATGGQFVQNFPDRTFKSMIIPLMQEDKRAGETTQKGFYIYDKNRKAKPDPEL
Sbjct: 541 LVGFGVAIATGGQFVQNFPDRTFKSMIIPLMQEDKRAGETTQKGFYIYDKNRKAKPDPEL 600

Query: 601 KKYIEKARTMSGISIDHKLTKLTEKDIIEMVFFPVVNEACRVLAEGIAVKAADLDIAGVM 660
           KKYIEKAR+MSGISID KLTKLTEKDIIEMVFFPVVNEACRVLAEGIAVKAADLDIAGVM
Sbjct: 601 KKYIEKARSMSGISIDPKLTKLTEKDIIEMVFFPVVNEACRVLAEGIAVKAADLDIAGVM 660

Query: 661 GMGFPPYRGGVMFWADSLGSKYIYSRLEEWSKHYGGFFKPCAYLAERAAQGATLVS 717
           GMGFPPYRGGVMFWADSLGSKYIYSRLEEWSK YGGFFKPCAYLAERAAQGATL S
Sbjct: 661 GMGFPPYRGGVMFWADSLGSKYIYSRLEEWSKRYGGFFKPCAYLAERAAQGATLSS 716

BLAST of CmaCh08G012320 vs. NCBI nr
Match: XP_023514320.1 (glyoxysomal fatty acid beta-oxidation multifunctional protein MFP-a-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1384.4 bits (3582), Expect = 0.0e+00
Identity = 705/716 (98.46%), Postives = 708/716 (98.88%), Query Frame = 0

Query: 1   MGSQAKGRTTLEVGADGIALITIINPPVNSLSFDVLFSLKESYEQALQREDVKAIVLTGA 60
           MGSQAKGRTTLEVGADGIALITIINPPVNSLSFDVLFSLKESYEQALQREDVKAIVLTGA
Sbjct: 1   MGSQAKGRTTLEVGADGIALITIINPPVNSLSFDVLFSLKESYEQALQREDVKAIVLTGA 60

Query: 61  RGKFSGGFDITAFGGLQGGKAREPRPGYISVDVITDIFEAARKPAVAAIDGLALGGGLEV 120
           RGKFSGGFDITAFGGLQGGKAREPRPGYISVDVITDIFEAARKPAVAAIDGLALGGGLEV
Sbjct: 61  RGKFSGGFDITAFGGLQGGKAREPRPGYISVDVITDIFEAARKPAVAAIDGLALGGGLEV 120

Query: 121 AMACHARVSTKTTLLGLPELQLGLIPGFGGTQRLPRLVGLSKALEMMLTSMPVKGEEAFS 180
           AMACHARVSTKT LLGLPELQLGLIPGFGGTQRLPRLVGLSKALEMMLTS PVKGEEAFS
Sbjct: 121 AMACHARVSTKTALLGLPELQLGLIPGFGGTQRLPRLVGLSKALEMMLTSKPVKGEEAFS 180

Query: 181 LGLVDAIVPSEELISTARKLALDISERRKPWIISLHRTDKLESLGDAREIFKFARAQIRK 240
           LGLVDAIVPSEELISTARKLALDISERRKPWIISLHRTDKLESLGDAREIFKFARAQIRK
Sbjct: 181 LGLVDAIVPSEELISTARKLALDISERRKPWIISLHRTDKLESLGDAREIFKFARAQIRK 240

Query: 241 QAPNLMHPLVCIDVVETGVVSGPRAGLWKEIEDFQVLLHADTSKSLINIFFAQRGTTKVP 300
           QAPNL HPLVCIDVVETGVVSGPRAGLWKEIEDFQVLLHADTSKSLINIFFAQRGTTKVP
Sbjct: 241 QAPNLTHPLVCIDVVETGVVSGPRAGLWKEIEDFQVLLHADTSKSLINIFFAQRGTTKVP 300

Query: 301 GVTDLGLKPRRIKKVAVVGGGLMGSGIATALVLSNYPVILKEVNDKFLKAGLDRVRANLQ 360
           GVTDLGLKPRRIKKVAVVGGGLMGSGIATALVLSNYPVILKEVNDKFLKAGL RVRANLQ
Sbjct: 301 GVTDLGLKPRRIKKVAVVGGGLMGSGIATALVLSNYPVILKEVNDKFLKAGLARVRANLQ 360

Query: 361 SRLRKGKMTPEKFEKTISLLDGALDYESFKDVDMVIEAVIENVSLKQQIFVDLEKYCPPH 420
           S+LRKGKMTPEKFEKTISLLDGALDYESFKDVDMVIEAVIENVSLKQQIFVDLEKYCPPH
Sbjct: 361 SQLRKGKMTPEKFEKTISLLDGALDYESFKDVDMVIEAVIENVSLKQQIFVDLEKYCPPH 420

Query: 421 CILATNTSTIDLELIGERTKSHDRIVGAHFFSPAHVMPLLEVVRTKRTAPQVIVDLIDVG 480
           CILATNTSTIDLELIGERTKSHDRIVGAHFFSPAHVMPLLEVVRTKRTAPQVIVDLIDVG
Sbjct: 421 CILATNTSTIDLELIGERTKSHDRIVGAHFFSPAHVMPLLEVVRTKRTAPQVIVDLIDVG 480

Query: 481 KKIKKAPVVVGNCTGFAVNRMFFPYTQAALLLVEHGVDPYQMDRAVFKFGMPMGPFRLID 540
           KKIKK PVVVGNCTGFAVNRMFFPYTQAALLLVEHGVDPYQ+DRAV KFGMPMGPFRLID
Sbjct: 481 KKIKKTPVVVGNCTGFAVNRMFFPYTQAALLLVEHGVDPYQIDRAVSKFGMPMGPFRLID 540

Query: 541 LVGFGVAIATGGQFVQNFPDRTFKSMIIPLMQEDKRAGETTQKGFYIYDKNRKAKPDPEL 600
           LVGFGVAIATGGQFVQNFPDRTFKSMIIPLMQEDKRAGETTQKGFYIYDKNRKAKPDPEL
Sbjct: 541 LVGFGVAIATGGQFVQNFPDRTFKSMIIPLMQEDKRAGETTQKGFYIYDKNRKAKPDPEL 600

Query: 601 KKYIEKARTMSGISIDHKLTKLTEKDIIEMVFFPVVNEACRVLAEGIAVKAADLDIAGVM 660
           KKYIEKAR+MSGISID KLTKLTEKDIIEMVFFPVVNEACRVLAEGIAVKAADLDIAGVM
Sbjct: 601 KKYIEKARSMSGISIDPKLTKLTEKDIIEMVFFPVVNEACRVLAEGIAVKAADLDIAGVM 660

Query: 661 GMGFPPYRGGVMFWADSLGSKYIYSRLEEWSKHYGGFFKPCAYLAERAAQGATLVS 717
           GMGFPPYRGGVMFWADSLGSKYIYSRLEEWSKHYGGFFKPCAYLAERAAQGATL S
Sbjct: 661 GMGFPPYRGGVMFWADSLGSKYIYSRLEEWSKHYGGFFKPCAYLAERAAQGATLSS 716

BLAST of CmaCh08G012320 vs. NCBI nr
Match: XP_022930239.1 (glyoxysomal fatty acid beta-oxidation multifunctional protein MFP-a-like [Cucurbita moschata] >XP_022930240.1 glyoxysomal fatty acid beta-oxidation multifunctional protein MFP-a-like [Cucurbita moschata])

HSP 1 Score: 1381.7 bits (3575), Expect = 0.0e+00
Identity = 704/716 (98.32%), Postives = 707/716 (98.74%), Query Frame = 0

Query: 1   MGSQAKGRTTLEVGADGIALITIINPPVNSLSFDVLFSLKESYEQALQREDVKAIVLTGA 60
           MGSQAKGRTTLEVGADGIALITIINPPVNSLSFDVLFSLKESYEQALQREDVKAIVLTGA
Sbjct: 1   MGSQAKGRTTLEVGADGIALITIINPPVNSLSFDVLFSLKESYEQALQREDVKAIVLTGA 60

Query: 61  RGKFSGGFDITAFGGLQGGKAREPRPGYISVDVITDIFEAARKPAVAAIDGLALGGGLEV 120
           RGKFSGGFDITAFGGLQGGKAREPRPGYISVDVITDIFEAARKPAVAAIDGLALGGGLEV
Sbjct: 61  RGKFSGGFDITAFGGLQGGKAREPRPGYISVDVITDIFEAARKPAVAAIDGLALGGGLEV 120

Query: 121 AMACHARVSTKTTLLGLPELQLGLIPGFGGTQRLPRLVGLSKALEMMLTSMPVKGEEAFS 180
           AMACHARVSTKT LLGLPELQLGLIPGFGGTQRLPRLVGLSKALEMMLTS PVKGEEAFS
Sbjct: 121 AMACHARVSTKTALLGLPELQLGLIPGFGGTQRLPRLVGLSKALEMMLTSKPVKGEEAFS 180

Query: 181 LGLVDAIVPSEELISTARKLALDISERRKPWIISLHRTDKLESLGDAREIFKFARAQIRK 240
           LGLVDAIVPSEELISTARKLALDISERRKPWIISLHRTDKLESLGDAREIFKFARAQIRK
Sbjct: 181 LGLVDAIVPSEELISTARKLALDISERRKPWIISLHRTDKLESLGDAREIFKFARAQIRK 240

Query: 241 QAPNLMHPLVCIDVVETGVVSGPRAGLWKEIEDFQVLLHADTSKSLINIFFAQRGTTKVP 300
           QAPNL HPLVCIDVVETGVVSGPRAGLWKEIEDFQVLLHADTSKSLINIFFAQRGTTKVP
Sbjct: 241 QAPNLTHPLVCIDVVETGVVSGPRAGLWKEIEDFQVLLHADTSKSLINIFFAQRGTTKVP 300

Query: 301 GVTDLGLKPRRIKKVAVVGGGLMGSGIATALVLSNYPVILKEVNDKFLKAGLDRVRANLQ 360
           GVTDLGLKPRRIKKVAVVGGGLMGSGIATALVLSNYPVILKEVNDKFLKAGLDRVRANLQ
Sbjct: 301 GVTDLGLKPRRIKKVAVVGGGLMGSGIATALVLSNYPVILKEVNDKFLKAGLDRVRANLQ 360

Query: 361 SRLRKGKMTPEKFEKTISLLDGALDYESFKDVDMVIEAVIENVSLKQQIFVDLEKYCPPH 420
           S+LRKGKMTPEKFEKTISLL GALDYESFKDVDMVIEAVIENVSLKQQIFVDLEKYCPPH
Sbjct: 361 SQLRKGKMTPEKFEKTISLLHGALDYESFKDVDMVIEAVIENVSLKQQIFVDLEKYCPPH 420

Query: 421 CILATNTSTIDLELIGERTKSHDRIVGAHFFSPAHVMPLLEVVRTKRTAPQVIVDLIDVG 480
           CILATNTSTIDLELIGERTKSHDRIVGAHFFSPAHVMPLLEVVRTKRTAPQVIVDLIDVG
Sbjct: 421 CILATNTSTIDLELIGERTKSHDRIVGAHFFSPAHVMPLLEVVRTKRTAPQVIVDLIDVG 480

Query: 481 KKIKKAPVVVGNCTGFAVNRMFFPYTQAALLLVEHGVDPYQMDRAVFKFGMPMGPFRLID 540
           KKIKK PVVVGNCTGFAVNRMFFPYTQAALLLVEHGVDPYQ+DRAV KFGMPMGPFRLID
Sbjct: 481 KKIKKTPVVVGNCTGFAVNRMFFPYTQAALLLVEHGVDPYQIDRAVSKFGMPMGPFRLID 540

Query: 541 LVGFGVAIATGGQFVQNFPDRTFKSMIIPLMQEDKRAGETTQKGFYIYDKNRKAKPDPEL 600
           LVGFGVAIATGGQFVQNFPDRTFKSMIIPLMQEDKRAGETTQKGFYIYDKNRKAKPDPEL
Sbjct: 541 LVGFGVAIATGGQFVQNFPDRTFKSMIIPLMQEDKRAGETTQKGFYIYDKNRKAKPDPEL 600

Query: 601 KKYIEKARTMSGISIDHKLTKLTEKDIIEMVFFPVVNEACRVLAEGIAVKAADLDIAGVM 660
           KKYIEKAR+MSGISID KLTKLTEKDIIEMVFFPVVNEACRVLAEGIAVKAADLDIAGVM
Sbjct: 601 KKYIEKARSMSGISIDPKLTKLTEKDIIEMVFFPVVNEACRVLAEGIAVKAADLDIAGVM 660

Query: 661 GMGFPPYRGGVMFWADSLGSKYIYSRLEEWSKHYGGFFKPCAYLAERAAQGATLVS 717
           GMGFPPYRGGVMFWADSLGSKYIYSRLEEWSK YGGFFKPCAYLAERAAQGATL S
Sbjct: 661 GMGFPPYRGGVMFWADSLGSKYIYSRLEEWSKRYGGFFKPCAYLAERAAQGATLSS 716

BLAST of CmaCh08G012320 vs. TAIR 10
Match: AT3G06860.1 (multifunctional protein 2 )

HSP 1 Score: 1116.7 bits (2887), Expect = 0.0e+00
Identity = 542/722 (75.07%), Postives = 632/722 (87.53%), Query Frame = 0

Query: 1   MGSQAKGRTTLEVGADGIALITIINPPVNSLSFDVLFSLKESYEQALQREDVKAIVLTGA 60
           M S+ KG+T +EVG DG+A+IT+INPPVNSLSFDVL++LK +YE+AL R DVKAIV+TGA
Sbjct: 1   MDSRTKGKTVMEVGGDGVAVITLINPPVNSLSFDVLYNLKSNYEEALSRNDVKAIVITGA 60

Query: 61  RGKFSGGFDITAFGGLQGGKAREPRPGYISVDVITDIFEAARKPAVAAIDGLALGGGLEV 120
           +G+FSGGFDI+ FG +Q G  +EP+ GYIS+D+ITD+ EAARKP+VAAIDGLALGGGLE+
Sbjct: 61  KGRFSGGFDISGFGEMQKGNVKEPKAGYISIDIITDLLEAARKPSVAAIDGLALGGGLEL 120

Query: 121 AMACHARVSTKTTLLGLPELQLGLIPGFGGTQRLPRLVGLSKALEMMLTSMPVKGEEAFS 180
           AMACHAR+S     LGLPELQLG+IPGFGGTQRLPRLVGL+KALEM+LTS PVK EE  S
Sbjct: 121 AMACHARISAPAAQLGLPELQLGVIPGFGGTQRLPRLVGLTKALEMILTSKPVKAEEGHS 180

Query: 181 LGLVDAIVPSEELISTARKLALDISERRKPWIISLHRTDKLESLGDAREIFKFARAQIRK 240
           LGL+DA+VP  EL++TAR+ ALDI  RRKPW+ S+ +TDKL  LG+AREI  FA+AQ  K
Sbjct: 181 LGLIDAVVPPAELVTTARRWALDIVGRRKPWVSSVSKTDKLPPLGEAREILTFAKAQTLK 240

Query: 241 QAPNLMHPLVCIDVVETGVVSGPRAGLWKEIEDFQVLLHADTSKSLINIFFAQRGTTKVP 300
           +APN+ HPL+C+D +E G+VSGPRAGL KE E    ++  DT+K LI++FF+QRGT KVP
Sbjct: 241 RAPNMKHPLMCLDAIEVGIVSGPRAGLEKEAEVASQVVKLDTTKGLIHVFFSQRGTAKVP 300

Query: 301 GVTDLGLKPRRIKKVAVVGGGLMGSGIATALVLSNYPVILKEVNDKFLKAGLDRVRANLQ 360
           GVTD GL PR+IKKVA++GGGLMGSGIATAL+LSNYPVILKEVN+KFL+AG+ RV+ANLQ
Sbjct: 301 GVTDRGLVPRKIKKVAIIGGGLMGSGIATALILSNYPVILKEVNEKFLEAGIGRVKANLQ 360

Query: 361 SRLRKGKMTPEKFEKTISLLDGALDYESFKDVDMVIEAVIENVSLKQQIFVDLEKYCPPH 420
           SR+RKG M+ EKFEKT+SLL G+LDYESF+DVDMVIEAVIEN+SLKQQIF DLEKYCP H
Sbjct: 361 SRVRKGSMSQEKFEKTMSLLKGSLDYESFRDVDMVIEAVIENISLKQQIFADLEKYCPQH 420

Query: 421 CILATNTSTIDLELIGERTKSHDRIVGAHFFSPAHVMPLLEVVRTKRTAPQVIVDLIDVG 480
           CILA+NTSTIDL  IGERTKS DRIVGAHFFSPAH+MPLLE+VRT  T+ QVIVDL+DVG
Sbjct: 421 CILASNTSTIDLNKIGERTKSQDRIVGAHFFSPAHIMPLLEIVRTNHTSAQVIVDLLDVG 480

Query: 481 KKIKKAPVVVGNCTGFAVNRMFFPYTQAALLLVEHGVDPYQMDRAVFKFGMPMGPFRLID 540
           KKIKK PVVVGNCTGFAVNRMFFPYTQAA+ LVE G DPY +DRA+ KFGMPMGPFRL D
Sbjct: 481 KKIKKTPVVVGNCTGFAVNRMFFPYTQAAMFLVECGADPYLIDRAISKFGMPMGPFRLCD 540

Query: 541 LVGFGVAIATGGQFVQNFPDRTFKSMIIPLMQEDKRAGETTQKGFYIYDKNRKAKPDPEL 600
           LVGFGVAIAT  QF++NF +RT+KSMIIPLMQEDKRAGE T+KGFY+YD  RKAKPDPEL
Sbjct: 541 LVGFGVAIATATQFIENFSERTYKSMIIPLMQEDKRAGEATRKGFYLYDDKRKAKPDPEL 600

Query: 601 KKYIEKARTMSGISIDHKLTKLTEKDIIEMVFFPVVNEACRVLAEGIAVKAADLDIAGVM 660
           KKYIEKAR++SG+ +D KL  L+EKDIIEM FFPVVNEACRV AEGIAVKAADLDIAG+M
Sbjct: 601 KKYIEKARSISGVKLDPKLANLSEKDIIEMTFFPVVNEACRVFAEGIAVKAADLDIAGIM 660

Query: 661 GMGFPPYRGGVMFWADSLGSKYIYSRLEEWSKHYGGFFKPCAYLAERAAQGATLVSKPQL 720
           GMGFPPYRGG+MFWADS+GSKYIYSRL+EWSK YG FFKPCA+LAER ++G  L+S P  
Sbjct: 661 GMGFPPYRGGIMFWADSIGSKYIYSRLDEWSKAYGEFFKPCAFLAERGSKG-VLLSAPVK 720

Query: 721 QS 723
           Q+
Sbjct: 721 QA 721

BLAST of CmaCh08G012320 vs. TAIR 10
Match: AT4G29010.1 (Enoyl-CoA hydratase/isomerase family )

HSP 1 Score: 814.3 bits (2102), Expect = 7.8e-236
Identity = 404/707 (57.14%), Postives = 529/707 (74.82%), Query Frame = 0

Query: 10  TLEVGADGIALITIINPPVNSLSFDVLFSLKESYEQALQREDVKAIVLTGARGKFSGGFD 69
           T+EVG DG+A+ITI NPPVNSL+  ++  LKE +  A QR DVKAIVL G  G+FSGGFD
Sbjct: 8   TMEVGNDGVAVITISNPPVNSLASPIISGLKEKFRDANQRNDVKAIVLIGNNGRFSGGFD 67

Query: 70  ITAFGGLQ--GGKAREPRPGYISVDVITDIFEAARKPAVAAIDGLALGGGLEVAMACHAR 129
           I  F  +   G  +  P    +SV+++ ++ E +RKP VAA++GLALGGGLE+AMACHAR
Sbjct: 68  INVFQQVHKTGDLSLMPE---VSVELVCNLMEDSRKPVVAAVEGLALGGGLELAMACHAR 127

Query: 130 VSTKTTLLGLPELQLGLIPGFGGTQRLPRLVGLSKALEMMLTSMPVKGEEAFSLGLVDAI 189
           V+     LGLPEL LG+IPGFGGTQRLPRLVGL+KA +M+L S  +  EE   LGL+DA+
Sbjct: 128 VAAPKAQLGLPELTLGVIPGFGGTQRLPRLVGLAKATDMILLSKSISSEEGHKLGLIDAL 187

Query: 190 VPSEELISTARKLALDISERRKPWIISLHRTDKLESLGDAREIFKFARAQIRKQAPNLMH 249
           VP  +++ST+RK ALDI+E RKP++ SLHRTDK+ SL +AR I K +R   +K APN+  
Sbjct: 188 VPPGDVLSTSRKWALDIAEGRKPFLQSLHRTDKIGSLSEARAILKNSRQLAKKIAPNMPQ 247

Query: 250 PLVCIDVVETGVVSGPRAGLWKEIEDFQVLLHADTSKSLINIFFAQRGTTKVPGVTDLGL 309
              CI+V+E G++ G  +G+ KE E F+ L+ +DT+K L+++FFAQR T+KVP VTD+GL
Sbjct: 248 HHACIEVIEEGIIHGGYSGVLKEAEVFKQLVLSDTAKGLVHVFFAQRATSKVPNVTDVGL 307

Query: 310 KPRRIKKVAVVGGGLMGSGIATALVLSNYPVILKEVNDKFLKAGLDRVRANLQSRLRKGK 369
           KPR IKKVAV+GGGLMGSGIATAL+LSN  V+LKE+N +FL  G+  V AN++S + +GK
Sbjct: 308 KPRPIKKVAVIGGGLMGSGIATALLLSNIRVVLKEINSEFLMKGIKSVEANMKSLVSRGK 367

Query: 370 MTPEKFEKTISLLDGALDYESFKDVDMVIEAVIENVSLKQQIFVDLEKYCPPHCILATNT 429
           +T +K  K +SL  G LDY  F DVDMVIEAVIEN+ LKQ IF ++EK C PHCILA+NT
Sbjct: 368 LTQDKAGKALSLFKGVLDYTEFNDVDMVIEAVIENIQLKQNIFKEIEKVCSPHCILASNT 427

Query: 430 STIDLELIGERTKSHDRIVGAHFFSPAHVMPLLEVVRTKRTAPQVIVDLIDVGKKIKKAP 489
           STIDL++IGE+T S DRIVGAHFFSPAH+MPLLE+VR+K T+ QVI+DL+ VGK IKK P
Sbjct: 428 STIDLDVIGEKTNSKDRIVGAHFFSPAHLMPLLEIVRSKNTSAQVILDLMAVGKAIKKVP 487

Query: 490 VVVGNCTGFAVNRMFFPYTQAALLLVEHGVDPYQMDRAVFKFGMPMGPFRLIDLVGFGVA 549
           VVVGNC GFAVNR FFPY+QAA +L   GVD +++D  +  FG+P+GPF+L DL G G+ 
Sbjct: 488 VVVGNCIGFAVNRTFFPYSQAAHMLANLGVDLFRIDSVITSFGLPLGPFQLGDLAGHGIG 547

Query: 550 IATGGQFVQNFPDRTFKSMIIPLMQEDKRAGETTQKGFYIYDKNRKAKPDPELKKYIEKA 609
           +A G  + + + DR F+S +  L+ +  R G+   +G+YIY+K  K KPDP +   +EK+
Sbjct: 548 LAVGPIYAKVYGDRMFRSPMTELLLKSGRNGKINGRGYYIYEKGSKPKPDPSVLSIVEKS 607

Query: 610 RTMSGISIDHKLTKLTEKDIIEMVFFPVVNEACRVLAEGIAVKAADLDIAGVMGMGFPPY 669
           R ++ I    K   +T+K+I+EM+ FPVVNEACRVL EG+ ++A+DLDIA V+GM FP Y
Sbjct: 608 RKLTNIMPGGKPISVTDKEIVEMILFPVVNEACRVLDEGVVIRASDLDIASVLGMSFPSY 667

Query: 670 RGGVMFWADSLGSKYIYSRLEEWSKHYGGFFKPCAYLAERAAQGATL 715
           RGG++FWAD++G KYIY RL++ S+ YG FFKP  YL ERA  G  L
Sbjct: 668 RGGIVFWADTVGPKYIYERLKKLSETYGSFFKPSRYLEERAMNGMLL 711

BLAST of CmaCh08G012320 vs. TAIR 10
Match: AT3G15290.1 (3-hydroxyacyl-CoA dehydrogenase family protein )

HSP 1 Score: 119.8 bits (299), Expect = 9.2e-27
Identity = 77/281 (27.40%), Postives = 135/281 (48.04%), Query Frame = 0

Query: 312 IKKVAVVGGGLMGSGIATALVLSNYPVILKEVNDKFLKAGLDRVRANLQSRLRKGKMTPE 371
           +K V VVG G MGSGIA     S   V L + +   L      + ++++  + KG ++ E
Sbjct: 4   MKSVGVVGAGQMGSGIAQLAATSGLDVWLMDADRDALSRATAAISSSVKRFVSKGLISKE 63

Query: 372 KFEKTISLLDGALDYESFKDVDMVIEAVIENVSLKQQIFVDLEKYCPPHCILATNTSTID 431
             +  +  L    + E     D+++EA++E+  +K+++F DL+       ILA+NTS+I 
Sbjct: 64  VGDDAMHRLRLTSNLEDLCSADIIVEAIVESEDIKKKLFKDLDGIAKSSAILASNTSSIS 123

Query: 432 LELIGERTKSHDRIVGAHFFSPAHVMPLLEVVRTKRTAPQVIVDLIDVGKKIKKAPVVVG 491
           +  +   T+   +++G HF +P  +M L+E++R   T+ +  +    + ++  K  V   
Sbjct: 124 ITRLASATRRPSQVIGMHFMNPPPIMKLVEIIRGADTSEETFLATKVLAERFGKTTVCSQ 183

Query: 492 NCTGFAVNRMFFPYTQAALLLVEHGVDPYQMDRAVFKFGM--PMGPFRLIDLVGFGVAIA 551
           +  GF VNR+  P    A   +  GV   +   +  K G   PMGP  L DL+G  V ++
Sbjct: 184 DYAGFVVNRILMPMINEAFHTLYTGVATKEDIDSGMKHGTNHPMGPLELADLIGLDVCLS 243

Query: 552 TGGQFVQNFPDRTFKSMIIPLMQEDKRAGETTQK-GFYIYD 590
                 +   D  +     PL+ +   AG   +K G  +YD
Sbjct: 244 VMKVLHEGLGDSKYAP--CPLLVQYVDAGRLGRKRGVGVYD 282

BLAST of CmaCh08G012320 vs. TAIR 10
Match: AT4G16210.1 (enoyl-CoA hydratase/isomerase A )

HSP 1 Score: 102.4 bits (254), Expect = 1.5e-21
Identity = 62/200 (31.00%), Postives = 110/200 (55.00%), Query Frame = 0

Query: 17  GIALITIINP-PVNSLSFDVLFSLKESYEQALQREDVKAIVLTGARGKFSGGFDITAFGG 76
           GIA+ITI  P  +NSL+  ++  L ++++     E V+ ++ TG+   F  G D+TA   
Sbjct: 18  GIAVITINRPKSLNSLTRAMMVDLAKAFKDMDSDESVQVVIFTGSGRSFCSGVDLTAAES 77

Query: 77  LQGGKAREPRPGYISVDVITDIFEAARKPAVAAIDGLALGGGLEVAMACHARVSTKTTLL 136
           +  G  ++P       D +  + E  RKP + AI+G A+  G E+A+AC   V+++    
Sbjct: 78  VFKGDVKDP-----ETDPVVQM-ERLRKPIIGAINGFAITAGFELALACDILVASRGAKF 137

Query: 137 GLPELQLGLIPGFGGTQRLPRLVGLSKALEMMLTSMPVKGEEAFSLGLVDAIVPSEELIS 196
                + G+ P +G +Q+L R++G +KA E+ LTSMP+  + A  LG V+ +V   E + 
Sbjct: 138 MDTHARFGIFPSWGLSQKLSRIIGANKAREVSLTSMPLTADVAGKLGFVNHVVEEGEALK 197

Query: 197 TARKLALDISERRKPWIISL 216
            AR++A  I +  +  ++ +
Sbjct: 198 KAREIAEAIIKNEQGMVLRI 211

BLAST of CmaCh08G012320 vs. TAIR 10
Match: AT4G16800.1 (ATP-dependent caseinolytic (Clp) protease/crotonase family protein )

HSP 1 Score: 100.1 bits (248), Expect = 7.5e-21
Identity = 68/200 (34.00%), Postives = 109/200 (54.50%), Query Frame = 0

Query: 14  GADGIALITIINPPV--NSLSFDVLFSLKESYEQALQREDVKAIVLTG-ARGKFSGGFDI 73
           G+D   +   ++ PV  N+++ ++L SL+ ++E   Q    + +++     G F  G D 
Sbjct: 50  GSDSGIIEVNLDRPVTKNAINKEMLKSLQNAFESIHQDNSARVVMIRSLVPGVFCAGAD- 109

Query: 74  TAFGGLQGGKAREPRPGYISVDVITDIF---EAARKPAVAAIDGLALGGGLEVAMACHAR 133
                L+  +   P   +  V+ +  +F   EA   P +AAI+G ALGGGLE+A+AC  R
Sbjct: 110 -----LKERRTMSPSEVHTYVNSLRYMFSFIEALSIPTIAAIEGAALGGGLEMALACDLR 169

Query: 134 VSTKTTLLGLPELQLGLIPGFGGTQRLPRLVGLSKALEMMLTSMPVKGEEAFSLGLVDAI 193
           +  +  + GLPE  L +IPG GGTQRL RLVG S + E++ T   +   EA + GLV+  
Sbjct: 170 ICGENAVFGLPETGLAIIPGAGGTQRLSRLVGRSVSKELIFTGRKIDAIEAANKGLVNIC 229

Query: 194 VPSEELISTARKLALDISER 208
           V + E    A ++A  I+E+
Sbjct: 230 VTAGEAHEKAIEMAQQINEK 243

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q396590.0e+0082.77Glyoxysomal fatty acid beta-oxidation multifunctional protein MFP-a OS=Cucumis s... [more]
Q9ZPI50.0e+0075.07Peroxisomal fatty acid beta-oxidation multifunctional protein MFP2 OS=Arabidopsi... [more]
O498090.0e+0074.79Glyoxysomal fatty acid beta-oxidation multifunctional protein MFP-a OS=Brassica ... [more]
Q9ZPI61.1e-23457.14Peroxisomal fatty acid beta-oxidation multifunctional protein AIM1 OS=Arabidopsi... [more]
Q8W1L61.1e-23457.14Peroxisomal fatty acid beta-oxidation multifunctional protein OS=Oryza sativa su... [more]
Match NameE-valueIdentityDescription
A0A6J1KLC60.0e+0099.863-hydroxyacyl-CoA dehydrogenase OS=Cucurbita maxima OX=3661 GN=LOC111494319 PE=3... [more]
A0A6J1EWF30.0e+0098.323-hydroxyacyl-CoA dehydrogenase OS=Cucurbita moschata OX=3662 GN=LOC111436753 PE... [more]
A0A6J1CBF00.0e+0091.763-hydroxyacyl-CoA dehydrogenase OS=Momordica charantia OX=3673 GN=LOC111009683 P... [more]
A0A0A0KI310.0e+0090.783-hydroxyacyl-CoA dehydrogenase OS=Cucumis sativus OX=3659 GN=Csa_6G319770 PE=3 ... [more]
A0A1S3C8F00.0e+0090.923-hydroxyacyl-CoA dehydrogenase OS=Cucumis melo OX=3656 GN=LOC103497841 PE=3 SV=... [more]
Match NameE-valueIdentityDescription
XP_023000007.10.0e+0099.86glyoxysomal fatty acid beta-oxidation multifunctional protein MFP-a-like [Cucurb... [more]
KAG6593976.10.0e+0098.46hypothetical protein SDJN03_13452, partial [Cucurbita argyrosperma subsp. sorori... [more]
KAG7026317.10.0e+0098.46hypothetical protein SDJN02_12818 [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_023514320.10.0e+0098.46glyoxysomal fatty acid beta-oxidation multifunctional protein MFP-a-like [Cucurb... [more]
XP_022930239.10.0e+0098.32glyoxysomal fatty acid beta-oxidation multifunctional protein MFP-a-like [Cucurb... [more]
Match NameE-valueIdentityDescription
AT3G06860.10.0e+0075.07multifunctional protein 2 [more]
AT4G29010.17.8e-23657.14Enoyl-CoA hydratase/isomerase family [more]
AT3G15290.19.2e-2727.403-hydroxyacyl-CoA dehydrogenase family protein [more]
AT4G16210.11.5e-2131.00enoyl-CoA hydratase/isomerase A [more]
AT4G16800.17.5e-2134.00ATP-dependent caseinolytic (Clp) protease/crotonase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.40.50.720coord: 308..490
e-value: 2.5E-63
score: 215.1
NoneNo IPR availableGENE3D1.10.1040.50coord: 497..721
e-value: 3.5E-72
score: 244.4
NoneNo IPR availableGENE3D3.90.226.10coord: 1..307
e-value: 1.3E-111
score: 374.7
NoneNo IPR availablePANTHERPTHR233093-HYDROXYACYL-COA DEHYROGENASEcoord: 5..717
NoneNo IPR availablePANTHERPTHR23309:SF42GLYOXYSOMAL FATTY ACID BETA-OXIDATION MFP-A PROTEINcoord: 5..717
NoneNo IPR availableCDDcd06558crotonase-likecoord: 16..204
e-value: 3.62328E-58
score: 193.546
IPR001753Enoyl-CoA hydratase/isomerasePFAMPF00378ECH_1coord: 16..206
e-value: 2.4E-39
score: 135.1
IPR0061763-hydroxyacyl-CoA dehydrogenase, NAD bindingPFAMPF027373HCDH_Ncoord: 314..492
e-value: 1.6E-58
score: 197.6
IPR0061083-hydroxyacyl-CoA dehydrogenase, C-terminalPFAMPF007253HCDHcoord: 495..588
e-value: 1.8E-19
score: 70.1
IPR018376Enoyl-CoA hydratase/isomerase, conserved sitePROSITEPS00166ENOYL_COA_HYDRATASEcoord: 106..126
IPR0061803-hydroxyacyl-CoA dehydrogenase, conserved sitePROSITEPS000673HCDHcoord: 492..517
IPR036291NAD(P)-binding domain superfamilySUPERFAMILY51735NAD(P)-binding Rossmann-fold domainscoord: 311..493
IPR0089276-phosphogluconate dehydrogenase-like, C-terminal domain superfamilySUPERFAMILY481796-phosphogluconate dehydrogenase C-terminal domain-likecoord: 495..606
IPR0089276-phosphogluconate dehydrogenase-like, C-terminal domain superfamilySUPERFAMILY481796-phosphogluconate dehydrogenase C-terminal domain-likecoord: 622..712
IPR029045ClpP/crotonase-like domain superfamilySUPERFAMILY52096ClpP/crotonasecoord: 10..302

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh08G012320.1CmaCh08G012320.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006631 fatty acid metabolic process
biological_process GO:0006635 fatty acid beta-oxidation
cellular_component GO:0009514 glyoxysome
molecular_function GO:0018812 3-hydroxyacyl-CoA dehydratase activity
molecular_function GO:0003857 3-hydroxyacyl-CoA dehydrogenase activity
molecular_function GO:0008692 3-hydroxybutyryl-CoA epimerase activity
molecular_function GO:0004165 dodecenoyl-CoA delta-isomerase activity
molecular_function GO:0004300 enoyl-CoA hydratase activity
molecular_function GO:0070403 NAD+ binding
molecular_function GO:0003824 catalytic activity
molecular_function GO:0016491 oxidoreductase activity
molecular_function GO:0016616 oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor