CmaCh08G010860 (gene) Cucurbita maxima (Rimu) v1.1

Overview


Name	CmaCh08G010860
Type	gene
Organism	Cucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Description	ethylene-responsive transcription factor 1B-like
Location	Cma_Chr08: 6782139 .. 6782825 (-)
RNA-Seq Expression	CmaCh08G010860
Synteny	CmaCh08G010860

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGACTTCTTCATTATTTCACTGCCAAGACTCCGACTTCTCGCCGGAATCTTCCTTGAGCTCCTTCGAATCTCTCCGATGGGAAGCTTTTCAAACAAACGACAACTCTCTGCTCTTTAACGAGAATGATTCCGGCGAAATGCTCCTTCACAACTTGATCTCAGAGCCCACCTCGTACCCGCCGGCGAATTCTCTCCGGCAGGACCCGATCAAGGAAGAGGAGGTTGATTCGCTCGGAGAAGAAGAACCCCACAAGCCAAAATCCTACAGAGGAGTCCGCCGGCGTCCATGGGGGAAATTCGCGGCGGAGATACGGGATTCAACTAGGCATGGAGTTCGGGTTTGGCTCGGAACGTTCGATAGCGCCGAGGCGGCTGCTTTGGCTTATGATCAAGCCGCTTTCTCGATGCGGGGCGCGGCCGCTGTTCTCAATTTTCCAGTGGAAAGAGTTCAGGAATCTCTCAAGGAGATGGAAATGATCAACTCCGCCGCATACGGCGGAGACGGCGAATCTCCGGTGGTTGCTTTGAAGAGAAAACATTCTATGAGGAATAAATCAAGAAGGAGAAAGAGTAAAAATAAAGAAGACATTGGGAAAACAGAGAGTAATGTGGTTGTTTTAGAAGATCTTGGCTCTGATTATTTGGAAGAACTCTTGGAGCTATCTGAGAATCTCCGGCCATGGTGA

mRNA sequence

ATGACTTCTTCATTATTTCACTGCCAAGACTCCGACTTCTCGCCGGAATCTTCCTTGAGCTCCTTCGAATCTCTCCGATGGGAAGCTTTTCAAACAAACGACAACTCTCTGCTCTTTAACGAGAATGATTCCGGCGAAATGCTCCTTCACAACTTGATCTCAGAGCCCACCTCGTACCCGCCGGCGAATTCTCTCCGGCAGGACCCGATCAAGGAAGAGGAGGTTGATTCGCTCGGAGAAGAAGAACCCCACAAGCCAAAATCCTACAGAGGAGTCCGCCGGCGTCCATGGGGGAAATTCGCGGCGGAGATACGGGATTCAACTAGGCATGGAGTTCGGGTTTGGCTCGGAACGTTCGATAGCGCCGAGGCGGCTGCTTTGGCTTATGATCAAGCCGCTTTCTCGATGCGGGGCGCGGCCGCTGTTCTCAATTTTCCAGTGGAAAGAGTTCAGGAATCTCTCAAGGAGATGGAAATGATCAACTCCGCCGCATACGGCGGAGACGGCGAATCTCCGGTGGTTGCTTTGAAGAGAAAACATTCTATGAGGAATAAATCAAGAAGGAGAAAGAGTAAAAATAAAGAAGACATTGGGAAAACAGAGAGTAATGTGGTTGTTTTAGAAGATCTTGGCTCTGATTATTTGGAAGAACTCTTGGAGCTATCTGAGAATCTCCGGCCATGGTGA

Coding sequence (CDS)

ATGACTTCTTCATTATTTCACTGCCAAGACTCCGACTTCTCGCCGGAATCTTCCTTGAGCTCCTTCGAATCTCTCCGATGGGAAGCTTTTCAAACAAACGACAACTCTCTGCTCTTTAACGAGAATGATTCCGGCGAAATGCTCCTTCACAACTTGATCTCAGAGCCCACCTCGTACCCGCCGGCGAATTCTCTCCGGCAGGACCCGATCAAGGAAGAGGAGGTTGATTCGCTCGGAGAAGAAGAACCCCACAAGCCAAAATCCTACAGAGGAGTCCGCCGGCGTCCATGGGGGAAATTCGCGGCGGAGATACGGGATTCAACTAGGCATGGAGTTCGGGTTTGGCTCGGAACGTTCGATAGCGCCGAGGCGGCTGCTTTGGCTTATGATCAAGCCGCTTTCTCGATGCGGGGCGCGGCCGCTGTTCTCAATTTTCCAGTGGAAAGAGTTCAGGAATCTCTCAAGGAGATGGAAATGATCAACTCCGCCGCATACGGCGGAGACGGCGAATCTCCGGTGGTTGCTTTGAAGAGAAAACATTCTATGAGGAATAAATCAAGAAGGAGAAAGAGTAAAAATAAAGAAGACATTGGGAAAACAGAGAGTAATGTGGTTGTTTTAGAAGATCTTGGCTCTGATTATTTGGAAGAACTCTTGGAGCTATCTGAGAATCTCCGGCCATGGTGA

Protein sequence

MTSSLFHCQDSDFSPESSLSSFESLRWEAFQTNDNSLLFNENDSGEMLLHNLISEPTSYPPANSLRQDPIKEEEVDSLGEEEPHKPKSYRGVRRRPWGKFAAEIRDSTRHGVRVWLGTFDSAEAAALAYDQAAFSMRGAAAVLNFPVERVQESLKEMEMINSAAYGGDGESPVVALKRKHSMRNKSRRRKSKNKEDIGKTESNVVVLEDLGSDYLEELLELSENLRPW

Homology

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BLAST of CmaCh08G010860 vs. ExPASy Swiss-Prot
Match: Q8LDC8 (Ethylene-responsive transcription factor 1B OS=Arabidopsis thaliana OX=3702 GN=ERF1B PE=1 SV=2)

HSP 1 Score: 198.7 bits (504), Expect = 6.9e-50
Identity = 126/221 (57.01%), Postives = 149/221 (67.42%), Query Frame = 0


Query: 11  SDFSPESSLSSFESLRWEAFQTNDNSLLFNENDSGEMLLHNLISEPTSYPPANSLRQD-P 70
           S FSPE S+ S     + +  +N+ SL FNENDS EM L+ LI + T     +S  QD P
Sbjct: 11  SGFSPEYSIGSSPD-SFSSSSSNNYSLPFNENDSEEMFLYGLIEQSTQQTYIDSDSQDLP 70

Query: 71  IKEEEVDSLGEEEPHKPKSYRGVRRRPWGKFAAEIRDSTRHGVRVWLGTFDSAEAAALAY 130
           IK   V S   E     KSYRGVRRRPWGKFAAEIRDSTR+G+RVWLGTF+SAE AALAY
Sbjct: 71  IK--SVSSRKSE-----KSYRGVRRRPWGKFAAEIRDSTRNGIRVWLGTFESAEEAALAY 130

Query: 131 DQAAFSMRGAAAVLNFPVERVQESLKEMEMINSAAYGGDGESPVVALKRKHSMRNKSRRR 190
           DQAAFSMRG++A+LNF  ERVQESL E++         DG SPVVALKRKHSMR +   +
Sbjct: 131 DQAAFSMRGSSAILNFSAERVQESLSEIKYTYE-----DGCSPVVALKRKHSMRRRMTNK 190

Query: 191 KSKNKEDIGKTE--SNVVVLEDLGSDYLEELLELSENLRPW 229
           K+K+ +   ++    NVVV EDLG  YLEELL  SEN   W
Sbjct: 191 KTKDSDFDHRSVKLDNVVVFEDLGEQYLEELLGSSENSGTW 218

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BLAST of CmaCh08G010860 vs. ExPASy Swiss-Prot
Match: A0A3Q7I5Y9 (Ethylene-response factor C3 OS=Solanum lycopersicum OX=4081 GN=ERF.C.3 PE=2 SV=1)

HSP 1 Score: 197.6 bits (501), Expect = 1.5e-49
Identity = 114/194 (58.76%), Postives = 144/194 (74.23%), Query Frame = 0


Query: 35  NSLLFNENDSGEMLLHNLISEPTSYPPANSLRQDPIKEEEVDSLGEEEPHKPKSYRGVRR 94
           NSL FN ND+ +MLL+NL++E +S    NS     IKEEEV S   EE  K K+YRGVR+
Sbjct: 14  NSLPFNVNDTQDMLLYNLVAEGSSQETVNSSSSYGIKEEEVTSY--EEERKDKNYRGVRK 73

Query: 95  RPWGKFAAEIRDSTRHGVRVWLGTFDSAEAAALAYDQAAFSMRGAAAVLNFPVERVQESL 154
           RPWGK+AAEIRDSTR+GVRVWLGTFD+AE AALAYDQAAF+MRG+ A+LNFPVE V+ESL
Sbjct: 74  RPWGKYAAEIRDSTRNGVRVWLGTFDNAEEAALAYDQAAFAMRGSMAILNFPVEIVKESL 133

Query: 155 KEMEMINSAAYGGDGESPVVALKRKHSMRNKSRRRKSKNKEDIGKTESNVVVLEDLGSDY 214
            EM+      + G+  SPV+ LK+++SMR KS  RK++ ++D       VVV EDLG++Y
Sbjct: 134 NEMK----CRFDGNC-SPVIELKKRYSMRRKSVSRKNRARKD-------VVVFEDLGAEY 193

Query: 215 LEELLELSENLRPW 229
           LEELL  SE++  W
Sbjct: 194 LEELLISSESITNW 193

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BLAST of CmaCh08G010860 vs. ExPASy Swiss-Prot
Match: Q9LND1 (Ethylene-responsive transcription factor ERF094 OS=Arabidopsis thaliana OX=3702 GN=ERF094 PE=2 SV=1)

HSP 1 Score: 165.2 bits (417), Expect = 8.4e-40
Identity = 119/248 (47.98%), Postives = 144/248 (58.06%), Query Frame = 0


Query: 12  DFSPE----SSLSSFESLRWEAFQTNDNSLLFNENDSGEMLLHNLISEPTS--YPPANSL 71
           +FSPE    SS SS ES  WE       S L    D        L+S PT        + 
Sbjct: 11  EFSPENSSSSSWSSQESFLWE------ESFLHQSFDQS-----FLLSSPTDNYCDDFFAF 70

Query: 72  RQDPIKEE--EVDSLGEEEPHKPKSYRGVRRRPWGKFAAEIRDSTRHGVRVWLGTFDSAE 131
               IKEE  E     EEE    KSYRGVR+RPWGKFAAEIRDSTR G+RVWLGTFD+AE
Sbjct: 71  ESSIIKEEGKEATVAAEEE---EKSYRGVRKRPWGKFAAEIRDSTRKGIRVWLGTFDTAE 130

Query: 132 AAALAYDQAAFSMRGAAAVLNFPVERVQESLKEMEMINSAAYGGDGESPVVALKRKHSMR 191
           AAALAYDQAAF+++G+ AVLNFP + V+ESL++ME +N      DGESPV+ALKRKHSMR
Sbjct: 131 AAALAYDQAAFALKGSLAVLNFPADVVEESLRKMENVNL----NDGESPVIALKRKHSMR 190

Query: 192 NKSRRRK------------------------------SKNKEDIGKTESN--VVVLEDLG 220
           N+ R +K                              S+ +  +   ESN  +VVLEDLG
Sbjct: 191 NRPRGKKKSSSSSTLTSSPSSSSSYSSSSSSSSLSSRSRKQSVVMTQESNTTLVVLEDLG 240

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BLAST of CmaCh08G010860 vs. ExPASy Swiss-Prot
Match: Q8VYM0 (Ethylene-responsive transcription factor 15 OS=Arabidopsis thaliana OX=3702 GN=ERF15 PE=2 SV=1)

HSP 1 Score: 160.6 bits (405), Expect = 2.1e-38
Identity = 108/238 (45.38%), Postives = 142/238 (59.66%), Query Frame = 0


Query: 14  SPESSLSSFESLRWEAFQTNDNSLLFNENDSGEMLLHN--LISEPTSYPPANSLRQDPIK 73
           SP S  SS ESL W     N++  L   ++       N     +  S+     + ++ I+
Sbjct: 11  SPSSWSSSQESLLW-----NESCFLDQSSEPQAFFCPNYDYSDDFFSFESPEMMIKEEIQ 70

Query: 74  EEEVDSLGEEEP---HKPKSYRGVRRRPWGKFAAEIRDSTRHGVRVWLGTFDSAEAAALA 133
             +V +  EEE     + +SYRGVR+RPWGKFAAEIRDSTR+G+RVWLGTFD AE AALA
Sbjct: 71  NGDVSNSEEEEKVGIDEERSYRGVRKRPWGKFAAEIRDSTRNGIRVWLGTFDKAEEAALA 130

Query: 134 YDQAAFSMRGAAAVLNFPVERVQESLKEMEMINSAAYGGDGESPVVALKRKHSMRNKSRR 193
           YDQAAF+ +G+ A LNFPVE V+ESLK+ME +N      DG SPV+ALKRKHS+RN+ R 
Sbjct: 131 YDQAAFATKGSLATLNFPVEVVRESLKKMENVNL----HDGGSPVMALKRKHSLRNRPRG 190

Query: 194 RK--------------------------SKNKEDIGKTES-NVVVLEDLGSDYLEELL 220
           +K                          S +K+ + K ES  +VV EDLG++YLE+LL
Sbjct: 191 KKRSSSSSSSSSNSSSCSSSSSTSSTSRSSSKQSVVKQESGTLVVFEDLGAEYLEQLL 239

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BLAST of CmaCh08G010860 vs. ExPASy Swiss-Prot
Match: O04681 (Pathogenesis-related genes transcriptional activator PTI5 OS=Solanum lycopersicum OX=4081 GN=PTI5 PE=2 SV=1)

HSP 1 Score: 116.3 bits (290), Expect = 4.5e-25
Identity = 69/128 (53.91%), Postives = 84/128 (65.62%), Query Frame = 0


Query: 32  TNDNSLLFNENDSGEMLLHNLISEPTSYPPANSLRQDPIKEEEVDSLGEEEPHKP----- 91
           T  + L  NENDS EM+L+ +++E      AN+L   P   +    L      +P     
Sbjct: 4   TPQSDLPLNENDSQEMVLYEVLNE------ANAL-NIPYLPQRNQLLPRNNILRPLQCIG 63

Query: 92  KSYRGVRRRPWGKFAAEIRDSTRHGVRVWLGTFDSAEAAALAYDQAAFSMRGAAAVLNFP 151
           K YRGVRRRPWGK+AAEIRDS RHG RVWLGTF++AE AALAYD+AAF MRGA A+LNFP
Sbjct: 64  KKYRGVRRRPWGKYAAEIRDSARHGARVWLGTFETAEEAALAYDRAAFRMRGAKALLNFP 123

Query: 152 VERVQESL 155
            E V  S+
Sbjct: 124 SEIVNASV 124

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BLAST of CmaCh08G010860 vs. ExPASy TrEMBL
Match: A0A6J1KG45 (ethylene-responsive transcription factor 1B-like OS=Cucurbita maxima OX=3661 GN=LOC111494786 PE=4 SV=1)

HSP 1 Score: 446.0 bits (1146), Expect = 9.1e-122
Identity = 228/228 (100.00%), Postives = 228/228 (100.00%), Query Frame = 0


Query: 1   MTSSLFHCQDSDFSPESSLSSFESLRWEAFQTNDNSLLFNENDSGEMLLHNLISEPTSYP 60
           MTSSLFHCQDSDFSPESSLSSFESLRWEAFQTNDNSLLFNENDSGEMLLHNLISEPTSYP
Sbjct: 1   MTSSLFHCQDSDFSPESSLSSFESLRWEAFQTNDNSLLFNENDSGEMLLHNLISEPTSYP 60

Query: 61  PANSLRQDPIKEEEVDSLGEEEPHKPKSYRGVRRRPWGKFAAEIRDSTRHGVRVWLGTFD 120
           PANSLRQDPIKEEEVDSLGEEEPHKPKSYRGVRRRPWGKFAAEIRDSTRHGVRVWLGTFD
Sbjct: 61  PANSLRQDPIKEEEVDSLGEEEPHKPKSYRGVRRRPWGKFAAEIRDSTRHGVRVWLGTFD 120

Query: 121 SAEAAALAYDQAAFSMRGAAAVLNFPVERVQESLKEMEMINSAAYGGDGESPVVALKRKH 180
           SAEAAALAYDQAAFSMRGAAAVLNFPVERVQESLKEMEMINSAAYGGDGESPVVALKRKH
Sbjct: 121 SAEAAALAYDQAAFSMRGAAAVLNFPVERVQESLKEMEMINSAAYGGDGESPVVALKRKH 180

Query: 181 SMRNKSRRRKSKNKEDIGKTESNVVVLEDLGSDYLEELLELSENLRPW 229
           SMRNKSRRRKSKNKEDIGKTESNVVVLEDLGSDYLEELLELSENLRPW
Sbjct: 181 SMRNKSRRRKSKNKEDIGKTESNVVVLEDLGSDYLEELLELSENLRPW 228

[Close]

BLAST of CmaCh08G010860 vs. ExPASy TrEMBL
Match: A0A6J1EWU7 (ethylene-responsive transcription factor 1B-like OS=Cucurbita moschata OX=3662 GN=LOC111436864 PE=4 SV=1)

HSP 1 Score: 427.9 bits (1099), Expect = 2.6e-116
Identity = 221/228 (96.93%), Postives = 223/228 (97.81%), Query Frame = 0


Query: 1   MTSSLFHCQDSDFSPESSLSSFESLRWEAFQTNDNSLLFNENDSGEMLLHNLISEPTSYP 60
           M+SSLFHCQDSDFSPESSLSSFESLRWEAFQ NDNSLLFNENDSGEMLLHNLISEPTSYP
Sbjct: 1   MSSSLFHCQDSDFSPESSLSSFESLRWEAFQKNDNSLLFNENDSGEMLLHNLISEPTSYP 60

Query: 61  PANSLRQDPIKEEEVDSLGEEEPHKPKSYRGVRRRPWGKFAAEIRDSTRHGVRVWLGTFD 120
             NSLRQDPIKEEEVDSLGEEEPHKPKSYRGVRRRPWGKFAAEIRDSTRHGVRVWLGTFD
Sbjct: 61  TGNSLRQDPIKEEEVDSLGEEEPHKPKSYRGVRRRPWGKFAAEIRDSTRHGVRVWLGTFD 120

Query: 121 SAEAAALAYDQAAFSMRGAAAVLNFPVERVQESLKEMEMINSAAYGGDGESPVVALKRKH 180
           SAEAAALAYDQAAFSMRGAAAVLNFPVERVQESLKEMEMINSAAYGGDGESPVVALKRKH
Sbjct: 121 SAEAAALAYDQAAFSMRGAAAVLNFPVERVQESLKEMEMINSAAYGGDGESPVVALKRKH 180

Query: 181 SMRNKSRRRKSKNKEDIGKTESNVVVLEDLGSDYLEELLELSENLRPW 229
           SMRNKS RRKSKNKE+IGKTESNVVVLEDLG DYLEELLELSENLRPW
Sbjct: 181 SMRNKS-RRKSKNKEEIGKTESNVVVLEDLGPDYLEELLELSENLRPW 227

[Close]

BLAST of CmaCh08G010860 vs. ExPASy TrEMBL
Match: A0A5D3CA48 (Ethylene-responsive transcription factor 1B-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1503G00540 PE=4 SV=1)

HSP 1 Score: 362.1 bits (928), Expect = 1.7e-96
Identity = 190/228 (83.33%), Postives = 202/228 (88.60%), Query Frame = 0


Query: 1   MTSSLFHCQDSDFSPESSLSSFESLRWEAFQTNDNSLLFNENDSGEMLLHNLISEPTSYP 60
           M SS FH Q+SD SP+SS SSFESL WE FQ ND+SL FN+NDSGEMLLH+LISE    P
Sbjct: 1   MNSSFFHSQNSDLSPQSSFSSFESLPWEGFQINDDSLPFNQNDSGEMLLHSLISEAYVDP 60

Query: 61  PANSLRQDPIKEEEVDSLGEEEPHKPKSYRGVRRRPWGKFAAEIRDSTRHGVRVWLGTFD 120
           P NS RQ PIK+EEVDSLGEE+PHK KSYRGVRRRPWGKFAAEIRDSTRHGVRVWLGTFD
Sbjct: 61  PENSPRQVPIKDEEVDSLGEEDPHKRKSYRGVRRRPWGKFAAEIRDSTRHGVRVWLGTFD 120

Query: 121 SAEAAALAYDQAAFSMRGAAAVLNFPVERVQESLKEMEMINSAAYGGDGESPVVALKRKH 180
           SAEAAALAYDQAAFSMRG+AAVLNFPVERVQESLKEMEMINSAA GGDG SPVVALKRKH
Sbjct: 121 SAEAAALAYDQAAFSMRGSAAVLNFPVERVQESLKEMEMINSAAEGGDGGSPVVALKRKH 180

Query: 181 SMRNKSRRRKSKNKEDIGKTESNVVVLEDLGSDYLEELLELSENLRPW 229
           SMR K  RRKSK  + IGK+E+NVVVLEDLGSDYLEELLE SEN+RPW
Sbjct: 181 SMRKKFTRRKSKEIDGIGKSENNVVVLEDLGSDYLEELLESSENVRPW 228

[Close]

BLAST of CmaCh08G010860 vs. ExPASy TrEMBL
Match: A0A1S3CDC8 (ethylene-responsive transcription factor 1B-like OS=Cucumis melo OX=3656 GN=LOC103499414 PE=4 SV=1)

HSP 1 Score: 362.1 bits (928), Expect = 1.7e-96
Identity = 190/228 (83.33%), Postives = 202/228 (88.60%), Query Frame = 0


Query: 1   MTSSLFHCQDSDFSPESSLSSFESLRWEAFQTNDNSLLFNENDSGEMLLHNLISEPTSYP 60
           M SS FH Q+SD SP+SS SSFESL WE FQ ND+SL FN+NDSGEMLLH+LISE    P
Sbjct: 1   MNSSFFHSQNSDLSPQSSFSSFESLPWEGFQINDDSLPFNQNDSGEMLLHSLISEAYVDP 60

Query: 61  PANSLRQDPIKEEEVDSLGEEEPHKPKSYRGVRRRPWGKFAAEIRDSTRHGVRVWLGTFD 120
           P NS RQ PIK+EEVDSLGEE+PHK KSYRGVRRRPWGKFAAEIRDSTRHGVRVWLGTFD
Sbjct: 61  PENSPRQVPIKDEEVDSLGEEDPHKRKSYRGVRRRPWGKFAAEIRDSTRHGVRVWLGTFD 120

Query: 121 SAEAAALAYDQAAFSMRGAAAVLNFPVERVQESLKEMEMINSAAYGGDGESPVVALKRKH 180
           SAEAAALAYDQAAFSMRG+AAVLNFPVERVQESLKEMEMINSAA GGDG SPVVALKRKH
Sbjct: 121 SAEAAALAYDQAAFSMRGSAAVLNFPVERVQESLKEMEMINSAAEGGDGGSPVVALKRKH 180

Query: 181 SMRNKSRRRKSKNKEDIGKTESNVVVLEDLGSDYLEELLELSENLRPW 229
           SMR K  RRKSK  + IGK+E+NVVVLEDLGSDYLEELLE SEN+RPW
Sbjct: 181 SMRKKFTRRKSKEIDGIGKSENNVVVLEDLGSDYLEELLESSENVRPW 228

[Close]

BLAST of CmaCh08G010860 vs. ExPASy TrEMBL
Match: A0A0A0LKN3 (AP2/ERF domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G177210 PE=4 SV=1)

HSP 1 Score: 360.5 bits (924), Expect = 5.1e-96
Identity = 190/228 (83.33%), Postives = 201/228 (88.16%), Query Frame = 0


Query: 1   MTSSLFHCQDSDFSPESSLSSFESLRWEAFQTNDNSLLFNENDSGEMLLHNLISEPTSYP 60
           M SS FH Q+SD SPESS SSFESLRWE FQ ND+SL FN+NDS EMLLH+LISE    P
Sbjct: 1   MNSSFFHSQNSDLSPESSFSSFESLRWEGFQINDHSLPFNQNDSDEMLLHSLISEAYVDP 60

Query: 61  PANSLRQDPIKEEEVDSLGEEEPHKPKSYRGVRRRPWGKFAAEIRDSTRHGVRVWLGTFD 120
             NS RQDPIK+EEVDSLGEE+PHK KSYRGVRRRPWGK+AAEIRDSTR+GVRVWLGTFD
Sbjct: 61  QENSPRQDPIKDEEVDSLGEEDPHKRKSYRGVRRRPWGKYAAEIRDSTRNGVRVWLGTFD 120

Query: 121 SAEAAALAYDQAAFSMRGAAAVLNFPVERVQESLKEMEMINSAAYGGDGESPVVALKRKH 180
           SAEAAALAYDQAAFSMRG+AAVLNFPVERVQESLKEMEMINSA  GGDG SPVVALKRKH
Sbjct: 121 SAEAAALAYDQAAFSMRGSAAVLNFPVERVQESLKEMEMINSAGEGGDGGSPVVALKRKH 180

Query: 181 SMRNKSRRRKSKNKEDIGKTESNVVVLEDLGSDYLEELLELSENLRPW 229
           SMR K  RRKSK  + IGKTE+NVVVLEDLGSDYLEELLE SENLRPW
Sbjct: 181 SMRKKFTRRKSKEIDRIGKTENNVVVLEDLGSDYLEELLESSENLRPW 228

[Close]

BLAST of CmaCh08G010860 vs. NCBI nr
Match: XP_023000546.1 (ethylene-responsive transcription factor 1B-like [Cucurbita maxima])

HSP 1 Score: 446.0 bits (1146), Expect = 1.9e-121
Identity = 228/228 (100.00%), Postives = 228/228 (100.00%), Query Frame = 0


Query: 1   MTSSLFHCQDSDFSPESSLSSFESLRWEAFQTNDNSLLFNENDSGEMLLHNLISEPTSYP 60
           MTSSLFHCQDSDFSPESSLSSFESLRWEAFQTNDNSLLFNENDSGEMLLHNLISEPTSYP
Sbjct: 1   MTSSLFHCQDSDFSPESSLSSFESLRWEAFQTNDNSLLFNENDSGEMLLHNLISEPTSYP 60

Query: 61  PANSLRQDPIKEEEVDSLGEEEPHKPKSYRGVRRRPWGKFAAEIRDSTRHGVRVWLGTFD 120
           PANSLRQDPIKEEEVDSLGEEEPHKPKSYRGVRRRPWGKFAAEIRDSTRHGVRVWLGTFD
Sbjct: 61  PANSLRQDPIKEEEVDSLGEEEPHKPKSYRGVRRRPWGKFAAEIRDSTRHGVRVWLGTFD 120

Query: 121 SAEAAALAYDQAAFSMRGAAAVLNFPVERVQESLKEMEMINSAAYGGDGESPVVALKRKH 180
           SAEAAALAYDQAAFSMRGAAAVLNFPVERVQESLKEMEMINSAAYGGDGESPVVALKRKH
Sbjct: 121 SAEAAALAYDQAAFSMRGAAAVLNFPVERVQESLKEMEMINSAAYGGDGESPVVALKRKH 180

Query: 181 SMRNKSRRRKSKNKEDIGKTESNVVVLEDLGSDYLEELLELSENLRPW 229
           SMRNKSRRRKSKNKEDIGKTESNVVVLEDLGSDYLEELLELSENLRPW
Sbjct: 181 SMRNKSRRRKSKNKEDIGKTESNVVVLEDLGSDYLEELLELSENLRPW 228

[Close]

BLAST of CmaCh08G010860 vs. NCBI nr
Match: KAG6593840.1 (Ethylene-responsive transcription factor 1B, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 433.3 bits (1113), Expect = 1.3e-117
Identity = 223/228 (97.81%), Postives = 225/228 (98.68%), Query Frame = 0


Query: 1   MTSSLFHCQDSDFSPESSLSSFESLRWEAFQTNDNSLLFNENDSGEMLLHNLISEPTSYP 60
           M+SSLFHCQDSDFSPESSLSSFESLRWEAFQTNDNSLLFNENDSGEMLLHNLISEPTSYP
Sbjct: 31  MSSSLFHCQDSDFSPESSLSSFESLRWEAFQTNDNSLLFNENDSGEMLLHNLISEPTSYP 90

Query: 61  PANSLRQDPIKEEEVDSLGEEEPHKPKSYRGVRRRPWGKFAAEIRDSTRHGVRVWLGTFD 120
           P NSLRQDPIKEEEVDSLGEEEPHKPKSYRGVRRRPWGKFAAEIRDSTRHGVRVWLGTFD
Sbjct: 91  PGNSLRQDPIKEEEVDSLGEEEPHKPKSYRGVRRRPWGKFAAEIRDSTRHGVRVWLGTFD 150

Query: 121 SAEAAALAYDQAAFSMRGAAAVLNFPVERVQESLKEMEMINSAAYGGDGESPVVALKRKH 180
           SAEAAALAYDQAAFSMRGAAAVLNFPVERVQESLKEMEMINSAAYGGDGESPVVALKRKH
Sbjct: 151 SAEAAALAYDQAAFSMRGAAAVLNFPVERVQESLKEMEMINSAAYGGDGESPVVALKRKH 210

Query: 181 SMRNKSRRRKSKNKEDIGKTESNVVVLEDLGSDYLEELLELSENLRPW 229
           SMRNKS RRKSKNKE+IGKTESNVVVLEDLG DYLEELLELSENLRPW
Sbjct: 211 SMRNKS-RRKSKNKEEIGKTESNVVVLEDLGPDYLEELLELSENLRPW 257

[Close]

BLAST of CmaCh08G010860 vs. NCBI nr
Match: XP_022930410.1 (ethylene-responsive transcription factor 1B-like [Cucurbita moschata] >KAG7026172.1 Ethylene-responsive transcription factor 1B, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 427.9 bits (1099), Expect = 5.3e-116
Identity = 221/228 (96.93%), Postives = 223/228 (97.81%), Query Frame = 0


Query: 1   MTSSLFHCQDSDFSPESSLSSFESLRWEAFQTNDNSLLFNENDSGEMLLHNLISEPTSYP 60
           M+SSLFHCQDSDFSPESSLSSFESLRWEAFQ NDNSLLFNENDSGEMLLHNLISEPTSYP
Sbjct: 1   MSSSLFHCQDSDFSPESSLSSFESLRWEAFQKNDNSLLFNENDSGEMLLHNLISEPTSYP 60

Query: 61  PANSLRQDPIKEEEVDSLGEEEPHKPKSYRGVRRRPWGKFAAEIRDSTRHGVRVWLGTFD 120
             NSLRQDPIKEEEVDSLGEEEPHKPKSYRGVRRRPWGKFAAEIRDSTRHGVRVWLGTFD
Sbjct: 61  TGNSLRQDPIKEEEVDSLGEEEPHKPKSYRGVRRRPWGKFAAEIRDSTRHGVRVWLGTFD 120

Query: 121 SAEAAALAYDQAAFSMRGAAAVLNFPVERVQESLKEMEMINSAAYGGDGESPVVALKRKH 180
           SAEAAALAYDQAAFSMRGAAAVLNFPVERVQESLKEMEMINSAAYGGDGESPVVALKRKH
Sbjct: 121 SAEAAALAYDQAAFSMRGAAAVLNFPVERVQESLKEMEMINSAAYGGDGESPVVALKRKH 180

Query: 181 SMRNKSRRRKSKNKEDIGKTESNVVVLEDLGSDYLEELLELSENLRPW 229
           SMRNKS RRKSKNKE+IGKTESNVVVLEDLG DYLEELLELSENLRPW
Sbjct: 181 SMRNKS-RRKSKNKEEIGKTESNVVVLEDLGPDYLEELLELSENLRPW 227

[Close]

BLAST of CmaCh08G010860 vs. NCBI nr
Match: XP_023514892.1 (ethylene-responsive transcription factor 1B-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 426.0 bits (1094), Expect = 2.0e-115
Identity = 220/228 (96.49%), Postives = 222/228 (97.37%), Query Frame = 0


Query: 1   MTSSLFHCQDSDFSPESSLSSFESLRWEAFQTNDNSLLFNENDSGEMLLHNLISEPTSYP 60
           M+SSLFHCQDSDFSPESSLSSFESLRWEAFQ NDNSLLFNENDSGEMLLHNLISEPTSYP
Sbjct: 1   MSSSLFHCQDSDFSPESSLSSFESLRWEAFQKNDNSLLFNENDSGEMLLHNLISEPTSYP 60

Query: 61  PANSLRQDPIKEEEVDSLGEEEPHKPKSYRGVRRRPWGKFAAEIRDSTRHGVRVWLGTFD 120
             NSLRQDPIKEEEVDSLGEEEPHKPKSYRGVRRRPWGKFAAEIRDSTRHGVRVWLGTFD
Sbjct: 61  TGNSLRQDPIKEEEVDSLGEEEPHKPKSYRGVRRRPWGKFAAEIRDSTRHGVRVWLGTFD 120

Query: 121 SAEAAALAYDQAAFSMRGAAAVLNFPVERVQESLKEMEMINSAAYGGDGESPVVALKRKH 180
           SAEAAALAYDQAAFSMRGAAA LNFPVERVQESLKEMEMINSAAYGGDGESPVVALKRKH
Sbjct: 121 SAEAAALAYDQAAFSMRGAAAFLNFPVERVQESLKEMEMINSAAYGGDGESPVVALKRKH 180

Query: 181 SMRNKSRRRKSKNKEDIGKTESNVVVLEDLGSDYLEELLELSENLRPW 229
           SMRNKS RRKSKNKE+IGKTESNVVVLEDLG DYLEELLELSENLRPW
Sbjct: 181 SMRNKS-RRKSKNKEEIGKTESNVVVLEDLGPDYLEELLELSENLRPW 227

[Close]

BLAST of CmaCh08G010860 vs. NCBI nr
Match: XP_008460636.1 (PREDICTED: ethylene-responsive transcription factor 1B-like [Cucumis melo] >KAA0065672.1 ethylene-responsive transcription factor 1B-like [Cucumis melo var. makuwa] >TYK08230.1 ethylene-responsive transcription factor 1B-like [Cucumis melo var. makuwa])

HSP 1 Score: 362.1 bits (928), Expect = 3.6e-96
Identity = 190/228 (83.33%), Postives = 202/228 (88.60%), Query Frame = 0


Query: 1   MTSSLFHCQDSDFSPESSLSSFESLRWEAFQTNDNSLLFNENDSGEMLLHNLISEPTSYP 60
           M SS FH Q+SD SP+SS SSFESL WE FQ ND+SL FN+NDSGEMLLH+LISE    P
Sbjct: 1   MNSSFFHSQNSDLSPQSSFSSFESLPWEGFQINDDSLPFNQNDSGEMLLHSLISEAYVDP 60

Query: 61  PANSLRQDPIKEEEVDSLGEEEPHKPKSYRGVRRRPWGKFAAEIRDSTRHGVRVWLGTFD 120
           P NS RQ PIK+EEVDSLGEE+PHK KSYRGVRRRPWGKFAAEIRDSTRHGVRVWLGTFD
Sbjct: 61  PENSPRQVPIKDEEVDSLGEEDPHKRKSYRGVRRRPWGKFAAEIRDSTRHGVRVWLGTFD 120

Query: 121 SAEAAALAYDQAAFSMRGAAAVLNFPVERVQESLKEMEMINSAAYGGDGESPVVALKRKH 180
           SAEAAALAYDQAAFSMRG+AAVLNFPVERVQESLKEMEMINSAA GGDG SPVVALKRKH
Sbjct: 121 SAEAAALAYDQAAFSMRGSAAVLNFPVERVQESLKEMEMINSAAEGGDGGSPVVALKRKH 180

Query: 181 SMRNKSRRRKSKNKEDIGKTESNVVVLEDLGSDYLEELLELSENLRPW 229
           SMR K  RRKSK  + IGK+E+NVVVLEDLGSDYLEELLE SEN+RPW
Sbjct: 181 SMRKKFTRRKSKEIDGIGKSENNVVVLEDLGSDYLEELLESSENVRPW 228

[Close]

BLAST of CmaCh08G010860 vs. TAIR 10
Match: AT3G23240.1 (ethylene response factor 1 )

HSP 1 Score: 198.7 bits (504), Expect = 4.9e-51
Identity = 126/221 (57.01%), Postives = 149/221 (67.42%), Query Frame = 0


Query: 11  SDFSPESSLSSFESLRWEAFQTNDNSLLFNENDSGEMLLHNLISEPTSYPPANSLRQD-P 70
           S FSPE S+ S     + +  +N+ SL FNENDS EM L+ LI + T     +S  QD P
Sbjct: 11  SGFSPEYSIGSSPD-SFSSSSSNNYSLPFNENDSEEMFLYGLIEQSTQQTYIDSDSQDLP 70

Query: 71  IKEEEVDSLGEEEPHKPKSYRGVRRRPWGKFAAEIRDSTRHGVRVWLGTFDSAEAAALAY 130
           IK   V S   E     KSYRGVRRRPWGKFAAEIRDSTR+G+RVWLGTF+SAE AALAY
Sbjct: 71  IK--SVSSRKSE-----KSYRGVRRRPWGKFAAEIRDSTRNGIRVWLGTFESAEEAALAY 130

Query: 131 DQAAFSMRGAAAVLNFPVERVQESLKEMEMINSAAYGGDGESPVVALKRKHSMRNKSRRR 190
           DQAAFSMRG++A+LNF  ERVQESL E++         DG SPVVALKRKHSMR +   +
Sbjct: 131 DQAAFSMRGSSAILNFSAERVQESLSEIKYTYE-----DGCSPVVALKRKHSMRRRMTNK 190

Query: 191 KSKNKEDIGKTE--SNVVVLEDLGSDYLEELLELSENLRPW 229
           K+K+ +   ++    NVVV EDLG  YLEELL  SEN   W
Sbjct: 191 KTKDSDFDHRSVKLDNVVVFEDLGEQYLEELLGSSENSGTW 218

[Close]

BLAST of CmaCh08G010860 vs. TAIR 10
Match: AT1G06160.1 (octadecanoid-responsive Arabidopsis AP2/ERF 59 )

HSP 1 Score: 165.2 bits (417), Expect = 6.0e-41
Identity = 119/248 (47.98%), Postives = 144/248 (58.06%), Query Frame = 0


Query: 12  DFSPE----SSLSSFESLRWEAFQTNDNSLLFNENDSGEMLLHNLISEPTS--YPPANSL 71
           +FSPE    SS SS ES  WE       S L    D        L+S PT        + 
Sbjct: 11  EFSPENSSSSSWSSQESFLWE------ESFLHQSFDQS-----FLLSSPTDNYCDDFFAF 70

Query: 72  RQDPIKEE--EVDSLGEEEPHKPKSYRGVRRRPWGKFAAEIRDSTRHGVRVWLGTFDSAE 131
               IKEE  E     EEE    KSYRGVR+RPWGKFAAEIRDSTR G+RVWLGTFD+AE
Sbjct: 71  ESSIIKEEGKEATVAAEEE---EKSYRGVRKRPWGKFAAEIRDSTRKGIRVWLGTFDTAE 130

Query: 132 AAALAYDQAAFSMRGAAAVLNFPVERVQESLKEMEMINSAAYGGDGESPVVALKRKHSMR 191
           AAALAYDQAAF+++G+ AVLNFP + V+ESL++ME +N      DGESPV+ALKRKHSMR
Sbjct: 131 AAALAYDQAAFALKGSLAVLNFPADVVEESLRKMENVNL----NDGESPVIALKRKHSMR 190

Query: 192 NKSRRRK------------------------------SKNKEDIGKTESN--VVVLEDLG 220
           N+ R +K                              S+ +  +   ESN  +VVLEDLG
Sbjct: 191 NRPRGKKKSSSSSTLTSSPSSSSSYSSSSSSSSLSSRSRKQSVVMTQESNTTLVVLEDLG 240

[Close]

BLAST of CmaCh08G010860 vs. TAIR 10
Match: AT2G31230.1 (ethylene-responsive element binding factor 15 )

HSP 1 Score: 160.6 bits (405), Expect = 1.5e-39
Identity = 108/238 (45.38%), Postives = 142/238 (59.66%), Query Frame = 0


Query: 14  SPESSLSSFESLRWEAFQTNDNSLLFNENDSGEMLLHN--LISEPTSYPPANSLRQDPIK 73
           SP S  SS ESL W     N++  L   ++       N     +  S+     + ++ I+
Sbjct: 11  SPSSWSSSQESLLW-----NESCFLDQSSEPQAFFCPNYDYSDDFFSFESPEMMIKEEIQ 70

Query: 74  EEEVDSLGEEEP---HKPKSYRGVRRRPWGKFAAEIRDSTRHGVRVWLGTFDSAEAAALA 133
             +V +  EEE     + +SYRGVR+RPWGKFAAEIRDSTR+G+RVWLGTFD AE AALA
Sbjct: 71  NGDVSNSEEEEKVGIDEERSYRGVRKRPWGKFAAEIRDSTRNGIRVWLGTFDKAEEAALA 130

Query: 134 YDQAAFSMRGAAAVLNFPVERVQESLKEMEMINSAAYGGDGESPVVALKRKHSMRNKSRR 193
           YDQAAF+ +G+ A LNFPVE V+ESLK+ME +N      DG SPV+ALKRKHS+RN+ R 
Sbjct: 131 YDQAAFATKGSLATLNFPVEVVRESLKKMENVNL----HDGGSPVMALKRKHSLRNRPRG 190

Query: 194 RK--------------------------SKNKEDIGKTES-NVVVLEDLGSDYLEELL 220
           +K                          S +K+ + K ES  +VV EDLG++YLE+LL
Sbjct: 191 KKRSSSSSSSSSNSSSCSSSSSTSSTSRSSSKQSVVKQESGTLVVFEDLGAEYLEQLL 239

[Close]

BLAST of CmaCh08G010860 vs. TAIR 10
Match: AT5G43410.1 (Integrase-type DNA-binding superfamily protein )

HSP 1 Score: 104.8 bits (260), Expect = 9.6e-23
Identity = 49/60 (81.67%), Postives = 54/60 (90.00%), Query Frame = 0


Query: 89  YRGVRRRPWGKFAAEIRDSTRHGVRVWLGTFDSAEAAALAYDQAAFSMRGAAAVLNFPVE 148
           YRGVRRRPWGK+AAEIRDS +HG RVWLGTFD+AE AA AYDQAA+SMRG AA+LNFP E
Sbjct: 15  YRGVRRRPWGKYAAEIRDSRKHGERVWLGTFDTAEEAARAYDQAAYSMRGQAAILNFPHE 74

[Close]

BLAST of CmaCh08G010860 vs. TAIR 10
Match: AT1G04370.1 (Ethylene-responsive element binding factor 14 )

HSP 1 Score: 102.8 bits (255), Expect = 3.7e-22
Identity = 48/60 (80.00%), Postives = 54/60 (90.00%), Query Frame = 0


Query: 89  YRGVRRRPWGKFAAEIRDSTRHGVRVWLGTFDSAEAAALAYDQAAFSMRGAAAVLNFPVE 148
           YRGVRRRPWGK+AAEIRDS +HG RVWLGTFD+AE AA AYD+AA+SMRG AA+LNFP E
Sbjct: 20  YRGVRRRPWGKYAAEIRDSRKHGERVWLGTFDTAEDAARAYDRAAYSMRGKAAILNFPHE 79

The following BLAST results are available for this feature:

BLAST of CmaCh08G010860 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8LDC8	6.9e-50	57.01	Ethylene-responsive transcription factor 1B OS=Arabidopsis thaliana OX=3702 GN=E...	[more]
A0A3Q7I5Y9	1.5e-49	58.76	Ethylene-response factor C3 OS=Solanum lycopersicum OX=4081 GN=ERF.C.3 PE=2 SV=1	[more]
Q9LND1	8.4e-40	47.98	Ethylene-responsive transcription factor ERF094 OS=Arabidopsis thaliana OX=3702 ...	[more]
Q8VYM0	2.1e-38	45.38	Ethylene-responsive transcription factor 15 OS=Arabidopsis thaliana OX=3702 GN=E...	[more]
O04681	4.5e-25	53.91	Pathogenesis-related genes transcriptional activator PTI5 OS=Solanum lycopersicu...	[more]

BLAST of CmaCh08G010860 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1KG45	9.1e-122	100.00	ethylene-responsive transcription factor 1B-like OS=Cucurbita maxima OX=3661 GN=...	[more]
A0A6J1EWU7	2.6e-116	96.93	ethylene-responsive transcription factor 1B-like OS=Cucurbita moschata OX=3662 G...	[more]
A0A5D3CA48	1.7e-96	83.33	Ethylene-responsive transcription factor 1B-like OS=Cucumis melo var. makuwa OX=...	[more]
A0A1S3CDC8	1.7e-96	83.33	ethylene-responsive transcription factor 1B-like OS=Cucumis melo OX=3656 GN=LOC1...	[more]
A0A0A0LKN3	5.1e-96	83.33	AP2/ERF domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G177210 PE=...	[more]

BLAST of CmaCh08G010860 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023000546.1	1.9e-121	100.00	ethylene-responsive transcription factor 1B-like [Cucurbita maxima]	[more]
KAG6593840.1	1.3e-117	97.81	Ethylene-responsive transcription factor 1B, partial [Cucurbita argyrosperma sub...	[more]
XP_022930410.1	5.3e-116	96.93	ethylene-responsive transcription factor 1B-like [Cucurbita moschata] >KAG702617...	[more]
XP_023514892.1	2.0e-115	96.49	ethylene-responsive transcription factor 1B-like [Cucurbita pepo subsp. pepo]	[more]
XP_008460636.1	3.6e-96	83.33	PREDICTED: ethylene-responsive transcription factor 1B-like [Cucumis melo] >KAA0...	[more]

BLAST of CmaCh08G010860 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G23240.1	4.9e-51	57.01	ethylene response factor 1	[more]
AT1G06160.1	6.0e-41	47.98	octadecanoid-responsive Arabidopsis AP2/ERF 59	[more]
AT2G31230.1	1.5e-39	45.38	ethylene-responsive element binding factor 15	[more]
AT5G43410.1	9.6e-23	81.67	Integrase-type DNA-binding superfamily protein	[more]
AT1G04370.1	3.7e-22	80.00	Ethylene-responsive element binding factor 14	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001471	AP2/ERF domain	PRINTS	PR00367	ETHRSPELEMNT	coord: 89..100 score: 72.73 coord: 112..128 score: 65.88
IPR001471	AP2/ERF domain	SMART	SM00380	rav1_2	coord: 88..152 e-value: 4.3E-36 score: 135.9
IPR001471	AP2/ERF domain	PFAM	PF00847	AP2	coord: 88..138 e-value: 1.1E-14 score: 54.4
IPR001471	AP2/ERF domain	PROSITE	PS51032	AP2_ERF	coord: 88..146 score: 23.235113
IPR001471	AP2/ERF domain	CDD	cd00018	AP2	coord: 87..147 e-value: 1.89399E-19 score: 76.9267
IPR036955	AP2/ERF domain superfamily	GENE3D	3.30.730.10	AP2/ERF domain	coord: 87..148 e-value: 3.5E-26 score: 93.1
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 177..198
None	No IPR available	PANTHER	PTHR31190:SF132	ETHYLENE-RESPONSIVE TRANSCRIPTION FACTOR 1B	coord: 29..221
IPR044808	Ethylene-responsive transcription factor	PANTHER	PTHR31190	DNA-BINDING DOMAIN	coord: 29..221
IPR016177	DNA-binding domain superfamily	SUPERFAMILY	54171	DNA-binding domain	coord: 88..148

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmaCh08G010860.1	CmaCh08G010860.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0009873	ethylene-activated signaling pathway
biological_process	GO:0006355	regulation of transcription, DNA-templated
cellular_component	GO:0016020	membrane
cellular_component	GO:0005634	nucleus
molecular_function	GO:0003677	DNA binding
molecular_function	GO:0003700	DNA-binding transcription factor activity