CmaCh08G010320 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh08G010320
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
DescriptionNon-lysosomal glucosylceramidase
LocationCma_Chr08: 6446763 .. 6455148 (-)
RNA-Seq ExpressionCmaCh08G010320
SyntenyCmaCh08G010320
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
TTTCAGAGGAGGAGAATCAAAAGGAGCAGCAGCTCCGTTCATATTCCGAATCTCAACCTTACAGCGACCGGACTGTGCGCCACTTGGCGATCAATCAGCCGGAGGTGAAACCAATTGATAGGTGGCTCTCAGGCCACGGGCCACGCTGCTCCCTAGTATCTAGCTTTCTGCGCCAATCAATTGGAGGCTTCTGTATTGTTTACTCTTTTTATTTTTTTTTCTTTTGGATTTTCTTTTGGCAAAGCTTAATTTGATTCGAATTCCGTGAAGTTCGGCTCTATTTTGTTCGTAGAGTGGACTGGAATTCAATTCGGTTTCTTGAGCGGCGTGTTGTTTGTTGGTTGTGTTTGTGCTGGACGGAATTCAATTCGGTTTCTTCAACGGCGTGTTCTTTTTTGGTTGTTTTTGTGCCGGATCGGGCACCGATCCTTGGATATTTTGGTTTTGGATTTGGATTTGGTGGTATGGAGGTTGGTAGGATGGGGAATGAGAGTTGTTTTTTGATTTCGAATTAAGGTAGTGATTTTCTTCAGCTTTGTTTTTGATTTGGCGTTTGCTGTACACTCCGATTTGGGGCTGGTACTTGTCGTTTTTTTCGTCCTTGATTGGAGCTGAAGGGAGTTCCGTGGCTTAAATTGACTTGGAAGAAGGATTTGATTTTCAGAAGGAATTGCGTTTACGCTAGCTTAGGCATTCTATATTTGGACCTCTGTGCTTGTTCTCGGCTATATATTCACTCATCATCTACAAGTGATTTGAATTTCTCAACATGGTTAGCGGAAATTTGTTTCACTGTAGAAAGAACTCTTGGCCGCCGGACGAATATATCAGCAAGTCGACTTTGCAGCTGGTAAGTTAATTGGAAATGCTTATTCTCTTCATCATTTCCTTGATAACACCATGAATGTTTGTGAAATTTTGACTCGATTGACAAATATTTTTTTCAATGTTCTTCTTCCCTTGCAGTTTGATTTTGATAGTGCTTCACCACCAGAACAAGCGTGGAGAAGGAAATTAAATGGCCATGCGAATCTTCTCAAAGAATTTAGTGTCACATTTGTTGAAGCAATTAAAATGGTAAGTGATGGGTTCTCGACATTTTTTGTTGCTGCGCAAGATATCATGTCTCATCAATATCTTCTTTTATTGTTCTTTCTTTTGAGTAATGTGCAACCCTACTGCTATAGGTTCGACTTGGTATTCGTTTGTGGTCTTACGTAAGGGAAGAGGCTTCTCAAGGAAGGGTAATTTCCTCTTAAATTAAAAAAACAGCTTATTTCAATACTTGGACTTTGAAACCAATAGCTTACTTAATTTGTGCAGAAAGCGCCAATTGATCCATTCACCAAAGAAGCTTGCAAACCTTCAGCATCTCAAGGGGTTCCACTCGGAGGAATGGGGTATGTTTCGGATCGATGGGCATTATACACGGTAGTTCGCTGTCCGACATTCAATTGTATATTCTTAATCTCATGGGATTTCTTTAAATGTAATACAGAAGTGGCAGTATATCCAGAGGTTTTAGAGGCGAGTTCAGGCAATGGCAAATTATTCCTGGTACATGTGAAGCTTCTCCTGTCATGGCCAATCAATTTTCTGTAAGCCCTACCCTTCGACTATCTTCTAGGATATTTTTTTACAAATGTATGAGTATGAAGTTGTATTTAAGAGAATATATAAATATTAGAAGAATGGGAACATTCCTATATGTATATTTTTTCCTTTACTGTCTGTCTCTGCACTTAAAACGTGTTTTGTTTTCGAAGTGGTTAATATGTTCTCACGGTTATGCAGATTTTTATATCTAGAGATGGAGGAAATAAAAAATATGCGTCTGTCTTGGCTCCTGGTCAACATGAAGGCATAGGGTGAGGCTATCAGCTTATGAAGCCTCATTTTTTAAAATTCCACTTCTGTAAAATGCTAGATTCTTAGCATCATTTTATCAGCTTAACATAACATGCATGCCAATTTGTTGATGCACGTCGTATTACACATTCAGATACTATCTAATCTTCCAAGTCCCCAAAGAAGAGCTAACTGTAGTTCACCTAGTTATCTGGAGGAATCAATATTCTGTAATGAAGTTAATGAACTATATGTGCTGTTGGTGTTATAGGGTTAGAAAAATATTTGAACATCTACCTCTAAAATAATCAAAGCAAGCCTTGTGTTCTCTTTTTGATTCGTACCATCCATCAGATACATATGGGGGATTTGTGCTAGCAAATCATGGAACCACTTGTTTCTAGTTTTAGCAAAAAATTTCATCTAATCAAAGTTGGGGCAGATTCTGAACATAATTCCCTATTGTAGGAAAGCTGGAGACTCGGGGATATCATCGTGGGGATGGAATCTTGACGGTCAGCATTCCACATATCATGCTTTGTTTCCTAGGGCATGGACAGTATATGATGGTATCATTCTGTTTTTTTTTTTTTTTCTTTCTTTCTTTTGGTTTAGCTATGTTTTCTGCCCTTTTTAACTTATCATTGGTTAATGGCAATTTTTTTTCTTTTTTTTTTTTCAGGTGAGCCTGATCCAGAACTTAAAGTTTCGTGCAGGCAAATATCACCATTTATACCGCATAATTATCGAGATAGCAGTCTTCCCACTGCTGTTTTTGTATATACGGTATGTTTTCTTAGTAATGATCTCAAGTTATGCTTCTAGCTATAAATGAAAATTATTTTTTATTTCCTTGAGCACCAAGATTTCTAAGTAGCTTTTGTATGAGGAAGGAAACTCAAATTTGTGTGTTTCCTTAGTGTTATTCTACGAACGGGGAACATCTTAAAGACAATCATGAGTTTTAGGAAACTCAAAAGAATCAGTTTTAGGAAGATTAAGCATTTGGATTTGATATTGTTCATAACATGATCCCACTAATTAGTTGCTGCATTCGTAGGGGGAACATCTTAAAGACAACTGATATTGATTTGTACTACTTTCATTTGAATTCTTTTGTTGGTTGTTCTATGTTGCATGAAATAGTTTTTAACCAAAAAATTCTCTCTCTAGTACTTTGATTTTAGAAGTAAGAAATGATGATTATCTCTACAATTGTGGTGTGTTCTCTGCGATACCTTTTTGATGCATTGCAGCATTCTATTATCAAGCCTTCTCAATTATGGATAGGTTTACTTGGTTTTAGTTCCTTCTCCTTGATATTCATTTTTGTATTTATTCTTGTAGTTGGTAAATACTGGAAAGGAAAGGGCGAAAGTGAGCCTTGTTTTTTCTTGGGCGGTAAGATATTTTCTTTATCACTACTGTAATGCTATTTCCTATGCTTACTGATCTAATCAACAATTATTTTGTATCTGCAGAATTCAATAGGAGGAATCTCACATTTATCTGGCGATCATGTGAATGAGCCATTTATGTGTGTAAAAATCCAAATAATATGTGATCCTTCCCTCTCTTTGCTTCCTTCTTTCATCCTGTCTAACGTCTGATATAATCTATTTTCCTTTGATAGAGGCGAGGATGGAGTATCTGGAGTGCTTCTACATCACAAGCAAGTCACCAAACCTTATACTCTATTTTACGGTTGGTTGGAAAGTATCTTGGTTAATTGATCCCATGTATTTGAACATAACCAGAGAGAGAGAGAGAGAGAGGAGGTTGAACACTACAGTAACATGCTTGTCATTGAATAGATATATAGATGTTAGAAAATTTGAAAGAAAAATGAGAGTTCTGTAATGTATCGAGACTACGTGATGTGCCATATCCAAACTTTAAGTTTCATGATCAGTGGACATTTGAAGCAATTTCTTAGAAGTTGATTGATATAAATCAAGTAGGCATTAGAATAGCGAGTTTTGCTCATGATGATTGTGGAAAGCTATTTAGAAACTGACAAGTTTATGTTTCCTCACGTATTGTCGATGAGTACTAGTTTTTCAATTCTGTTTCTTATAACGCACAAGTTAGTTGTTGCATTTTGTTCCTTTGTCTGACAGCTTGTGTATATGGTAGGAGCTTGAGTATATGTTAGGCCTGTAAAGAATTTAAAAGACTTATGCATTTGTTTCAGGACTGCGAAAGGGAATCCTCCCGTTACTTTTGCCATAGCTGCATGTGAGACGCAAAATGTTAGTGTGACAGTTTTGCCATCTTTTGGACTCTCTGAAGGAAGTGGCGTCACAGCAAAGGACATGTGGGATAAAATGGTGCAGGTGAGAAACTTCTTTCTACAGCCTTTTGTGGGGATGAGCAAAAGTTCACTCAGTTTTTGTTCCTATCTTATTATTGTGTTTTGATGGCCTTTCAATATAAATATAATATATTAAAAAGCATATTCAATTGTTGAAAGGATGGGCAATTTGATCGTGGTAACTTCAGTTCGGGACCAAGCGTGCCTTCCTCACCTGGAGAGACACTTTGTGCTGCAGTTGCAGCCTCTGCATGGGTGGAGCCTCATGGAAAATGCACTGTCGCATTTTCTCTTTCATGGTCATCTCCAAAAGTGAAGTTTTTGAAGGGATTCTCATATCATAGGTAGGTATTTGTATTTTTTTTTTTTTTATTACAAACCAAACATTCATTAAGATGAGTGAGGCAATTAAAAAACAAAAGGCCATATTTGGTAGTTATTAGTAAGATATGTATTTGTCTTCATTTTTTCTTTTCAGATTCAAGTGTTTGGTAATTATTAAATAGAATCTTTGATTTGGTTTAAAATCATCAGCGAATGTCCACCATATTTTTGCCTGCCGAGTCCATGGAACTCTTTTGATCATCTTCATACCTCGTGTATATGCTCATGTATACAGTTTGGGGATTATGTTAGATATCAAGGAAAATTGATTTTGGTTTCAAAAGATACGCCACTTCTGATGAATAAGTGGAAATATTACCTTGTTAATTTATGGCAATGTCATTTTTATGTGTGGTCACAACCAGAAAGGATCCATATAAACCAATTATCCAAGCGTTCTCTTGACTTTTTGGGCTATATTTCAAGTGTGCGATTAAATCCTTCAGTGGTATGGAGTCGGATGCTAGAAAATTCATTTCTAATAGATAATGCTACCAAGAAAATCGATACACTAGTTCCTATTATTACTCTGCTTGGATCATTGGCTAAAGCGAAATTTTGTAATGTGCTAGGGCAGAGTTTATTCTTATAAACTAAGAACGTATATAAGTTATCATGAAAAAATGTGTTATATGGAATTATGTTTGTTCAATGTTGGACAATATAAAGTCTAGTCTTGCTCTGTGTGTTTCAGGAGGTACACAAAGTTTTACGGTACTTCTGAAAAGGCTGCTCAGACTTTGGCACATGATGCACTTACAAGTAAGCATCTCTGACTTATATTGACCTTGATCCGACAAACTTTTAAACAATGTTCTGTTTAGGATGACCTGTACATTTTTATTCTCTCGATGTACTTCTTAATCATATACTTCACATATTGCACTGATTCTTTAAATTCAATGAACTAAGCAGGATGATAAATTTTATGTCTAATATCCTTCTTAAATCATCAACAGATTATAAAAGATGGGAAGAGGAGATTGAGAAATGGCAGAGGCCTATCCTCATGGATGAGAGGTTACCTGAATGGTAATAATTGTTCCATCGTTGTTAATTGTATGAATATTTTGTGGAGTTTGTTTATTCTTGCCTGGTTCCTTCAGGGAAAACCCCCTTCAACACAAACTCTCTAATTCATGCTTTTAAAATTTCAACATATTAAAGATGGGTTACGAATGATGTAAGGGCATTCTTGGAACAGGTGAAGTTTGTTATTTAGCATTGGAAAAAATGTCTGTTTCTAAGGTTTATCAGAGACTACTATAGATTTTTAGGTGACTTGTCTAGACCGTTGGAAGTAAATATTCTATGGCTATATATTTCTCAACTGGTTCTTGACGCTTGTATCATTCATGTTGGATTAGCCATGTGACCAAAAATTAATCAAGTGTGGCCTAAATGCACAAAAAAAATAAATCAGAAACCATTTTAGAAACCGAATCCTTCGACAGAGTATTCCTATCACTGATACGTGTAGTTTCTGTATTTCCTCAGTGTCCCAAGGACATGAGCCACTTTTTAAATACGATTTACTTCTATGGCTTATGAGCGACATCTATTTTCTGCAGGTATAAGTTCACATTATTTAACGAGCTTTACTTTCTGGTTGCTGGTGGCACAGTCTGGATTGGTACTCAGCTACTTTAACTTCTCTTCTATGAACATTTAACTATTCATCTTTCTAACGGTTACAATATAACTTGTGTTACTTCTATGGTACCTACAGATTCTTCCGTAGTGGGTAAAAAAATGGCAAATGACCACGATGAACTAGTAGCTAGAATAAAAGAGGATGATATAAAAGCTACAGAAGCTAAAGTTAGTGGCAGGGGAGAAGAAGTCTCCAGGACTACGACCACCACTACTCTTAATGATTTTTCTGGTGTTGAATTTGATGATGAAGAAAATTCTACTTCTAGTTCCCATGTAAGTGAAGATGAGTTAATGGTTCCATTGAAAAGTGGCTACACTGACCGCTCTTATCAAACATACAAAGTAATGGACCCGGGAAACACTGAGGAAGATGTTGGTAGGTTTCTGTACTTGGAAGGAGTTGAATATGTAATGTGGTGCACGTATGATGTGCATTTTTATGCATCCTTTGCTCTTCTCGAGTTATTTCCCAAGATTGAACTCAATATTCAACGGGACTTTGCCAAAGCAGTCTTATCAGAAGACGGAAGAAAAGTGAAGTTTCTTGCCGATGGGAAATATGGAATCCGTAAAGTTAGAGGTGCCGTTCCACATGACCTTGGGACACATGATCCTTGGAATGAAATGAACGCTTACAACATTCACGATACAAGCAGGTGGAAGGATTTGAACACAAAGTTTGTGCTTCAGGTTTATAGAGATTTTGCTGCAACCCGTGACATGTCATTTGGAGTCGATGTTTGGCCGTCTGTTCGGGCTGCGATAGAATACATGGAACAATTTGACAGAGATGGTGACGGTGTAATTGAGAACGATGGATTTCCGGACCAGACCTATGATACTTGGACTGTTCATGGTATTAGTGCATACTGTGGCTGTTTGTGGGTTGCCGCTTTACAAGCTGCAGCAGCAATGGCCCATGAACTGGGCGACCAAGAGTTTGCTGAAACGTGCAAGAGCAAATTTCTAAAAGCCAAACCAGTTTTGGAAGCGAAACTGTGGAATGGTTCCTATTTTAACTATGACAGTGGATCAAGTAGTAATAGTAAATCAATACAAGCTGATCAACTAGCAGGGCAATGGTATACAGCATCCTCTGGTTTGCCTCCTCTGTTTGATGACTTCAAAATCAAAAGTGCTCTAAAAAAAATCTACGACTTCAATGTGATGAAGATTAGAGGGGGTAGGATGGGTGCTGTAAATGGGATGCATCCTAATGGAAAGGTGGATGAGACCTGCATGCAATCTCGTGAAATATGGACTGGTGTTACCTATGGTGTTGCAGCTACAATGATCCTTGCCGGTATGGAGGAGGAAGCATTTAAAACTGCGGAGGGTATATTCCTTGCTGGGTGGTCAGAAGAGGGATTTGGGTAAGTAAAAACTCACACTAATCAATTCCACTTCAGATTGCTCTTACTATTTGTGCCACCCTGTTTCTACTAAATATTATTAATTGAAACTGTCTACTTTGCAACTTATAAAGAATTTATCAAATGCCTGCTACCACACAACACTTGTAATCAGAGATGTTAGGTTTCGAGGGAATGTCAAAGAGTTCTTAATTTTAAGCAAACCAAGCATAAACCAATCAAATGTGTTTCCGCTCACCTTCTATTGTATTTGTTTTTAGGAGTTCAGAGAATGGGCTATAGGACTTGAACCTCAAATCTCTTGGCTGACGATATCTGTCTTAACCGATTGAACTATGCTTAGATTAGTGAATTTTGTCCCTTCTATTTGAGATGCTGATGTGTACTTTTTCTTTGATTCTATGTAGTGTGCTTCCATTTGAGTGTGAAGTCTTTTCAGTTATACAATCTAGGACATAACACCATTTTCCTATCATAATATCAATCTTCCCGTTATTCTTTCACAAAGCTCCTTTCAGTTGATCTTATTTCCTTCTCTACTGCCCACAGGTACTGGTTCCAGACACCTGAGGCATGGTCCACGGACGGGCACTACAGATCCCTCATATATATGAGACCACTGTCTATCTGGGGTATGCAGTGGGCATTATCCCTACCAAAGGCGATACTCGAGGCCCCAAAAATCAACGTAATGGACAGAATTCATGTATCCTCTGCCAGCACAAAGTTCTTTCACCATGAAACTGGTGTCAGAAGGATCGCTACAAAAGCTAAGTGTTTTGGTGATTCTGTTTTCAATTGTGCATGCTGA

mRNA sequence

TTTCAGAGGAGGAGAATCAAAAGGAGCAGCAGCTCCGTTCATATTCCGAATCTCAACCTTACAGCGACCGGACTGTGCGCCACTTGGCGATCAATCAGCCGGAGGTGAAACCAATTGATAGGTGGCTCTCAGGCCACGGGCCACGCTGCTCCCTAGTATCTAGCTTTCTGCGCCAATCAATTGGAGGCTTCTGTATTGTTTACTCTTTTTATTTTTTTTTCTTTTGGATTTTCTTTTGGCAAAGCTTAATTTGATTCGAATTCCGTGAAGTTCGGCTCTATTTTGTTCGTAGAGTGGACTGGAATTCAATTCGGTTTCTTGAGCGGCGTGTTGTTTGTTGGTTGTGTTTGTGCTGGACGGAATTCAATTCGGTTTCTTCAACGGCGTGTTCTTTTTTGGTTGTTTTTGTGCCGGATCGGGCACCGATCCTTGGATATTTTGGTTTTGGATTTGGATTTGGTGGTATGGAGGTTGGTAGGATGGGGAATGAGAGTTGTTTTTTGATTTCGAATTAAGGTAGTGATTTTCTTCAGCTTTGTTTTTGATTTGGCGTTTGCTGTACACTCCGATTTGGGGCTGGTACTTGTCGTTTTTTTCGTCCTTGATTGGAGCTGAAGGGAGTTCCGTGGCTTAAATTGACTTGGAAGAAGGATTTGATTTTCAGAAGGAATTGCGTTTACGCTAGCTTAGGCATTCTATATTTGGACCTCTGTGCTTGTTCTCGGCTATATATTCACTCATCATCTACAAGTGATTTGAATTTCTCAACATGGTTAGCGGAAATTTGTTTCACTGTAGAAAGAACTCTTGGCCGCCGGACGAATATATCAGCAAGTCGACTTTGCAGCTGTTTGATTTTGATAGTGCTTCACCACCAGAACAAGCGTGGAGAAGGAAATTAAATGGCCATGCGAATCTTCTCAAAGAATTTAGTGTCACATTTGTTGAAGCAATTAAAATGGTTCGACTTGGTATTCGTTTGTGGTCTTACGTAAGGGAAGAGGCTTCTCAAGGAAGGAAAGCGCCAATTGATCCATTCACCAAAGAAGCTTGCAAACCTTCAGCATCTCAAGGGGTTCCACTCGGAGGAATGGGAAGTGGCAGTATATCCAGAGGTTTTAGAGGCGAGTTCAGGCAATGGCAAATTATTCCTGGTACATGTGAAGCTTCTCCTGTCATGGCCAATCAATTTTCTATTTTTATATCTAGAGATGGAGGAAATAAAAAATATGCGTCTGTCTTGGCTCCTGGTCAACATGAAGGCATAGGGAAAGCTGGAGACTCGGGGATATCATCGTGGGGATGGAATCTTGACGGTCAGCATTCCACATATCATGCTTTGTTTCCTAGGGCATGGACAGTATATGATGGTGAGCCTGATCCAGAACTTAAAGTTTCGTGCAGGCAAATATCACCATTTATACCGCATAATTATCGAGATAGCAGTCTTCCCACTGCTGTTTTTGTATATACGTTGGTAAATACTGGAAAGGAAAGGGCGAAAGTGAGCCTTGTTTTTTCTTGGGCGAATTCAATAGGAGGAATCTCACATTTATCTGGCGATCATGTGAATGAGCCATTTATAGGCGAGGATGGAGTATCTGGAGTGCTTCTACATCACAAGACTGCGAAAGGGAATCCTCCCGTTACTTTTGCCATAGCTGCATGTGAGACGCAAAATGTTAGTGTGACAGTTTTGCCATCTTTTGGACTCTCTGAAGGAAGTGGCGTCACAGCAAAGGACATGTGGGATAAAATGGTGCAGGATGGGCAATTTGATCGTGGTAACTTCAGTTCGGGACCAAGCGTGCCTTCCTCACCTGGAGAGACACTTTGTGCTGCAGTTGCAGCCTCTGCATGGGTGGAGCCTCATGGAAAATGCACTGTCGCATTTTCTCTTTCATGGTCATCTCCAAAAGTGAAGTTTTTGAAGGGATTCTCATATCATAGGAGGTACACAAAGTTTTACGGTACTTCTGAAAAGGCTGCTCAGACTTTGGCACATGATGCACTTACAAATTATAAAAGATGGGAAGAGGAGATTGAGAAATGGCAGAGGCCTATCCTCATGGATGAGAGGTTACCTGAATGGTATAAGTTCACATTATTTAACGAGCTTTACTTTCTGGTTGCTGGTGGCACAGTCTGGATTGATTCTTCCGTAGTGGGTAAAAAAATGGCAAATGACCACGATGAACTAGTAGCTAGAATAAAAGAGGATGATATAAAAGCTACAGAAGCTAAAGTTAGTGGCAGGGGAGAAGAAGTCTCCAGGACTACGACCACCACTACTCTTAATGATTTTTCTGGTGTTGAATTTGATGATGAAGAAAATTCTACTTCTAGTTCCCATGTAAGTGAAGATGAGTTAATGGTTCCATTGAAAAGTGGCTACACTGACCGCTCTTATCAAACATACAAAGTAATGGACCCGGGAAACACTGAGGAAGATGTTGGTAGGTTTCTGTACTTGGAAGGAGTTGAATATGTAATGTGGTGCACGTATGATGTGCATTTTTATGCATCCTTTGCTCTTCTCGAGTTATTTCCCAAGATTGAACTCAATATTCAACGGGACTTTGCCAAAGCAGTCTTATCAGAAGACGGAAGAAAAGTGAAGTTTCTTGCCGATGGGAAATATGGAATCCGTAAAGTTAGAGGTGCCGTTCCACATGACCTTGGGACACATGATCCTTGGAATGAAATGAACGCTTACAACATTCACGATACAAGCAGGTGGAAGGATTTGAACACAAAGTTTGTGCTTCAGGTTTATAGAGATTTTGCTGCAACCCGTGACATGTCATTTGGAGTCGATGTTTGGCCGTCTGTTCGGGCTGCGATAGAATACATGGAACAATTTGACAGAGATGGTGACGGTGTAATTGAGAACGATGGATTTCCGGACCAGACCTATGATACTTGGACTGTTCATGGTATTAGTGCATACTGTGGCTGTTTGTGGGTTGCCGCTTTACAAGCTGCAGCAGCAATGGCCCATGAACTGGGCGACCAAGAGTTTGCTGAAACGTGCAAGAGCAAATTTCTAAAAGCCAAACCAGTTTTGGAAGCGAAACTGTGGAATGGTTCCTATTTTAACTATGACAGTGGATCAAGTAGTAATAGTAAATCAATACAAGCTGATCAACTAGCAGGGCAATGGTATACAGCATCCTCTGGTTTGCCTCCTCTGTTTGATGACTTCAAAATCAAAAGTGCTCTAAAAAAAATCTACGACTTCAATGTGATGAAGATTAGAGGGGGTAGGATGGGTGCTGTAAATGGGATGCATCCTAATGGAAAGGTGGATGAGACCTGCATGCAATCTCGTGAAATATGGACTGGTGTTACCTATGGTGTTGCAGCTACAATGATCCTTGCCGGTATGGAGGAGGAAGCATTTAAAACTGCGGAGGGTATATTCCTTGCTGGGTGGTCAGAAGAGGGATTTGGGTACTGGTTCCAGACACCTGAGGCATGGTCCACGGACGGGCACTACAGATCCCTCATATATATGAGACCACTGTCTATCTGGGGTATGCAGTGGGCATTATCCCTACCAAAGGCGATACTCGAGGCCCCAAAAATCAACGTAATGGACAGAATTCATGTATCCTCTGCCAGCACAAAGTTCTTTCACCATGAAACTGGTGTCAGAAGGATCGCTACAAAAGCTAAGTGTTTTGGTGATTCTGTTTTCAATTGTGCATGCTGA

Coding sequence (CDS)

ATGGTTAGCGGAAATTTGTTTCACTGTAGAAAGAACTCTTGGCCGCCGGACGAATATATCAGCAAGTCGACTTTGCAGCTGTTTGATTTTGATAGTGCTTCACCACCAGAACAAGCGTGGAGAAGGAAATTAAATGGCCATGCGAATCTTCTCAAAGAATTTAGTGTCACATTTGTTGAAGCAATTAAAATGGTTCGACTTGGTATTCGTTTGTGGTCTTACGTAAGGGAAGAGGCTTCTCAAGGAAGGAAAGCGCCAATTGATCCATTCACCAAAGAAGCTTGCAAACCTTCAGCATCTCAAGGGGTTCCACTCGGAGGAATGGGAAGTGGCAGTATATCCAGAGGTTTTAGAGGCGAGTTCAGGCAATGGCAAATTATTCCTGGTACATGTGAAGCTTCTCCTGTCATGGCCAATCAATTTTCTATTTTTATATCTAGAGATGGAGGAAATAAAAAATATGCGTCTGTCTTGGCTCCTGGTCAACATGAAGGCATAGGGAAAGCTGGAGACTCGGGGATATCATCGTGGGGATGGAATCTTGACGGTCAGCATTCCACATATCATGCTTTGTTTCCTAGGGCATGGACAGTATATGATGGTGAGCCTGATCCAGAACTTAAAGTTTCGTGCAGGCAAATATCACCATTTATACCGCATAATTATCGAGATAGCAGTCTTCCCACTGCTGTTTTTGTATATACGTTGGTAAATACTGGAAAGGAAAGGGCGAAAGTGAGCCTTGTTTTTTCTTGGGCGAATTCAATAGGAGGAATCTCACATTTATCTGGCGATCATGTGAATGAGCCATTTATAGGCGAGGATGGAGTATCTGGAGTGCTTCTACATCACAAGACTGCGAAAGGGAATCCTCCCGTTACTTTTGCCATAGCTGCATGTGAGACGCAAAATGTTAGTGTGACAGTTTTGCCATCTTTTGGACTCTCTGAAGGAAGTGGCGTCACAGCAAAGGACATGTGGGATAAAATGGTGCAGGATGGGCAATTTGATCGTGGTAACTTCAGTTCGGGACCAAGCGTGCCTTCCTCACCTGGAGAGACACTTTGTGCTGCAGTTGCAGCCTCTGCATGGGTGGAGCCTCATGGAAAATGCACTGTCGCATTTTCTCTTTCATGGTCATCTCCAAAAGTGAAGTTTTTGAAGGGATTCTCATATCATAGGAGGTACACAAAGTTTTACGGTACTTCTGAAAAGGCTGCTCAGACTTTGGCACATGATGCACTTACAAATTATAAAAGATGGGAAGAGGAGATTGAGAAATGGCAGAGGCCTATCCTCATGGATGAGAGGTTACCTGAATGGTATAAGTTCACATTATTTAACGAGCTTTACTTTCTGGTTGCTGGTGGCACAGTCTGGATTGATTCTTCCGTAGTGGGTAAAAAAATGGCAAATGACCACGATGAACTAGTAGCTAGAATAAAAGAGGATGATATAAAAGCTACAGAAGCTAAAGTTAGTGGCAGGGGAGAAGAAGTCTCCAGGACTACGACCACCACTACTCTTAATGATTTTTCTGGTGTTGAATTTGATGATGAAGAAAATTCTACTTCTAGTTCCCATGTAAGTGAAGATGAGTTAATGGTTCCATTGAAAAGTGGCTACACTGACCGCTCTTATCAAACATACAAAGTAATGGACCCGGGAAACACTGAGGAAGATGTTGGTAGGTTTCTGTACTTGGAAGGAGTTGAATATGTAATGTGGTGCACGTATGATGTGCATTTTTATGCATCCTTTGCTCTTCTCGAGTTATTTCCCAAGATTGAACTCAATATTCAACGGGACTTTGCCAAAGCAGTCTTATCAGAAGACGGAAGAAAAGTGAAGTTTCTTGCCGATGGGAAATATGGAATCCGTAAAGTTAGAGGTGCCGTTCCACATGACCTTGGGACACATGATCCTTGGAATGAAATGAACGCTTACAACATTCACGATACAAGCAGGTGGAAGGATTTGAACACAAAGTTTGTGCTTCAGGTTTATAGAGATTTTGCTGCAACCCGTGACATGTCATTTGGAGTCGATGTTTGGCCGTCTGTTCGGGCTGCGATAGAATACATGGAACAATTTGACAGAGATGGTGACGGTGTAATTGAGAACGATGGATTTCCGGACCAGACCTATGATACTTGGACTGTTCATGGTATTAGTGCATACTGTGGCTGTTTGTGGGTTGCCGCTTTACAAGCTGCAGCAGCAATGGCCCATGAACTGGGCGACCAAGAGTTTGCTGAAACGTGCAAGAGCAAATTTCTAAAAGCCAAACCAGTTTTGGAAGCGAAACTGTGGAATGGTTCCTATTTTAACTATGACAGTGGATCAAGTAGTAATAGTAAATCAATACAAGCTGATCAACTAGCAGGGCAATGGTATACAGCATCCTCTGGTTTGCCTCCTCTGTTTGATGACTTCAAAATCAAAAGTGCTCTAAAAAAAATCTACGACTTCAATGTGATGAAGATTAGAGGGGGTAGGATGGGTGCTGTAAATGGGATGCATCCTAATGGAAAGGTGGATGAGACCTGCATGCAATCTCGTGAAATATGGACTGGTGTTACCTATGGTGTTGCAGCTACAATGATCCTTGCCGGTATGGAGGAGGAAGCATTTAAAACTGCGGAGGGTATATTCCTTGCTGGGTGGTCAGAAGAGGGATTTGGGTACTGGTTCCAGACACCTGAGGCATGGTCCACGGACGGGCACTACAGATCCCTCATATATATGAGACCACTGTCTATCTGGGGTATGCAGTGGGCATTATCCCTACCAAAGGCGATACTCGAGGCCCCAAAAATCAACGTAATGGACAGAATTCATGTATCCTCTGCCAGCACAAAGTTCTTTCACCATGAAACTGGTGTCAGAAGGATCGCTACAAAAGCTAAGTGTTTTGGTGATTCTGTTTTCAATTGTGCATGCTGA

Protein sequence

MVSGNLFHCRKNSWPPDEYISKSTLQLFDFDSASPPEQAWRRKLNGHANLLKEFSVTFVEAIKMVRLGIRLWSYVREEASQGRKAPIDPFTKEACKPSASQGVPLGGMGSGSISRGFRGEFRQWQIIPGTCEASPVMANQFSIFISRDGGNKKYASVLAPGQHEGIGKAGDSGISSWGWNLDGQHSTYHALFPRAWTVYDGEPDPELKVSCRQISPFIPHNYRDSSLPTAVFVYTLVNTGKERAKVSLVFSWANSIGGISHLSGDHVNEPFIGEDGVSGVLLHHKTAKGNPPVTFAIAACETQNVSVTVLPSFGLSEGSGVTAKDMWDKMVQDGQFDRGNFSSGPSVPSSPGETLCAAVAASAWVEPHGKCTVAFSLSWSSPKVKFLKGFSYHRRYTKFYGTSEKAAQTLAHDALTNYKRWEEEIEKWQRPILMDERLPEWYKFTLFNELYFLVAGGTVWIDSSVVGKKMANDHDELVARIKEDDIKATEAKVSGRGEEVSRTTTTTTLNDFSGVEFDDEENSTSSSHVSEDELMVPLKSGYTDRSYQTYKVMDPGNTEEDVGRFLYLEGVEYVMWCTYDVHFYASFALLELFPKIELNIQRDFAKAVLSEDGRKVKFLADGKYGIRKVRGAVPHDLGTHDPWNEMNAYNIHDTSRWKDLNTKFVLQVYRDFAATRDMSFGVDVWPSVRAAIEYMEQFDRDGDGVIENDGFPDQTYDTWTVHGISAYCGCLWVAALQAAAAMAHELGDQEFAETCKSKFLKAKPVLEAKLWNGSYFNYDSGSSSNSKSIQADQLAGQWYTASSGLPPLFDDFKIKSALKKIYDFNVMKIRGGRMGAVNGMHPNGKVDETCMQSREIWTGVTYGVAATMILAGMEEEAFKTAEGIFLAGWSEEGFGYWFQTPEAWSTDGHYRSLIYMRPLSIWGMQWALSLPKAILEAPKINVMDRIHVSSASTKFFHHETGVRRIATKAKCFGDSVFNCAC
Homology
BLAST of CmaCh08G010320 vs. ExPASy Swiss-Prot
Match: Q5M868 (Non-lysosomal glucosylceramidase OS=Rattus norvegicus OX=10116 GN=Gba2 PE=2 SV=2)

HSP 1 Score: 627.5 bits (1617), Expect = 2.6e-178
Identity = 363/885 (41.02%), Postives = 480/885 (54.24%), Query Frame = 0

Query: 55  SVTFVEAIKMVRLGIRLWSYVREEASQGRKAP-IDPFTKEACKPSASQGVPLGGMGSGSI 114
           +V+    +K   +G+R   +   +    +K P ID F     +     G PLGG+G G+I
Sbjct: 96  NVSLSNLVKHFGMGLRYLKWWYRKTQVEKKTPFIDMFNSVPLR--QIYGCPLGGIGGGTI 155

Query: 115 SRGFRGEFRQWQIIPGTCEASPVMANQFSIFISRDGGNKKYASVLAPGQHEGIGKAGDSG 174
           +RG+RG+F +WQ+ PG  +   V+A+QF + + RD G   Y  VL+            S 
Sbjct: 156 TRGWRGQFCRWQLNPGMYQHQTVIADQFIVCLRRD-GRTVYQQVLS--------LELPSV 215

Query: 175 ISSWGWNLDGQHSTYHALFPRAWTVYDGEPDPELKVSCRQISPFIPHNYRDSSLPTAVFV 234
           + SW W L G  + YHAL+PRAWTVY   P   + ++CRQI+P +PH+Y+DSSLP  VFV
Sbjct: 216 LRSWNWGLCGYFAFYHALYPRAWTVYQ-LPGQNVTLTCRQITPILPHDYQDSSLPVGVFV 275

Query: 235 YTLVNTGKERAKVSLVFSWANSIGGISHLSGDHVNEPF-IGEDG--VSGVLLHHKTAKGN 294
           + + N G E   VS++FS  N +GG    +G   NEPF + +DG  V G+LLHH T    
Sbjct: 276 WDVENEGDETLDVSIMFSMRNGLGGEDDAAGGLWNEPFRLEQDGTTVQGLLLHHPTPPN- 335

Query: 295 PPVTFAIAACETQNVSVTVLPSFGLSEGSGVTAKDMWDKMVQDGQFDRGNFSSGPSVPSS 354
            P T A+AA  T + +VT   +F        T + +W  ++QDGQ D     +G S P+ 
Sbjct: 336 -PYTMAVAARHTADTTVTYTTAFDPDS----TGQQVWQDLLQDGQLDS---PAGQSTPTQ 395

Query: 355 PGETLCAAVAASAWVEPHGKCTVAFSLSWSSPKVKF-LKGFSYHRRYTKFYGTSEKAAQT 414
            GE +  AV AS+ + P G+C + FSL+W  P++ F  KG  ++RRYT+F+G+    A  
Sbjct: 396 RGEGVAGAVCASSKLLPRGRCCLEFSLAWDMPRIMFGAKGQVHYRRYTRFFGSDGDVAPA 455

Query: 415 LAHDALTNYKRWEEEIEKWQRPILMDERLPEWYKFTLFNELYFLVAGGTVWIDSSVVGKK 474
           L+H AL  Y  WE  I  WQ P+L D  LP WYK  LFNELYFL  GGTVW++       
Sbjct: 456 LSHYALCQYAGWENSISAWQNPVLDDRSLPAWYKSALFNELYFLADGGTVWLE------- 515

Query: 475 MANDHDELVARIKEDDIKATEAKVSGRGEEVSRTTTTTTLNDFSGVEFDDEENSTSSSHV 534
                                                                      V
Sbjct: 516 -----------------------------------------------------------V 575

Query: 535 SEDELMVPLKSGYTDRSYQTYKVMDPGNTEEDVGRFLYLEGVEYVMWCTYDVHFYASFAL 594
            ED L   L        YQ   ++      +D GRF YLEG EY M+ TYDVHFYASFAL
Sbjct: 576 PEDSLPEELGGS----MYQLRPIL------QDYGRFGYLEGQEYRMYNTYDVHFYASFAL 635

Query: 595 LELFPKIELNIQRDFAKAVLSEDGRKVKFLADGKYGIRKVRGAVPHDLGTHD--PWNEMN 654
           + L+PK+EL++Q D A A   ED  + ++L  G     K R  +PHD+G  D  PW  +N
Sbjct: 636 VMLWPKLELSLQYDMALATFKEDLTRRRYLMSGVVAPVKRRNVIPHDIGDPDDEPWLRVN 695

Query: 655 AYNIHDTSRWKDLNTKFVLQVYRDFAATRDMSFGVDVWPSVRAAIEYMEQFDRDGDGVIE 714
           AY IHDT+ WKDLN KFVLQVYRD+  T D  F  D+WP   A +E   +FD+D DG+IE
Sbjct: 696 AYLIHDTADWKDLNLKFVLQVYRDYYLTGDQGFLKDMWPVCLAVMESEMKFDKDQDGLIE 755

Query: 715 NDGFPDQTYDTWTVHGISAYCGCLWVAALQAAAAMAHELGDQEFAETCKSKFLKAKPVLE 774
           N G+ DQTYD W   G SAYCG LW+AA+     MA   G Q+  +   S   + +   E
Sbjct: 756 NGGYADQTYDGWVTTGPSAYCGGLWLAAVAVMVQMAVLCGAQDVQDKFSSILCRGREAYE 815

Query: 775 AKLWNGSYFNYDSGSSSNSKSIQADQLAGQWYTASSGL----PPLFDDFKIKSALKKIYD 834
             LWNG Y+NYDS S   S+S+ +DQ AGQW+  + GL      +F    +  ALK I++
Sbjct: 816 RLLWNGRYYNYDSSSQPQSRSVMSDQCAGQWFLRACGLGEGDTEVFPTLHVVRALKTIFE 875

Query: 835 FNVMKIRGGRMGAVNGMHPNGKVDETCMQSREIWTGVTYGVAATMILAGMEEEAFKTAEG 894
            NV    GG MGAVNGM P+G  D + +QS E+W GV YG+AATMI  G+  E F+TAEG
Sbjct: 876 LNVQAFAGGAMGAVNGMQPHGVPDRSSVQSDEVWVGVVYGLAATMIQEGLTWEGFRTAEG 881

Query: 895 IFLAGWSEEGFGYWFQTPEAWSTDGHYRSLIYMRPLSIWGMQWAL 929
            +   W  E  G  FQTPEA+     +RSL YMRPLSIW MQ AL
Sbjct: 936 CYRTVW--ERLGLAFQTPEAYCQQRVFRSLAYMRPLSIWAMQLAL 881

BLAST of CmaCh08G010320 vs. ExPASy Swiss-Prot
Match: Q9HCG7 (Non-lysosomal glucosylceramidase OS=Homo sapiens OX=9606 GN=GBA2 PE=1 SV=2)

HSP 1 Score: 620.2 bits (1598), Expect = 4.1e-176
Identity = 362/885 (40.90%), Postives = 483/885 (54.58%), Query Frame = 0

Query: 55  SVTFVEAIKMVRLGIRLWSYVREEASQGRKAP-IDPFTKEACKPSASQGVPLGGMGSGSI 114
           +V+    IK + +G+R   +   +    +K P ID       +     G PLGG+G G+I
Sbjct: 105 NVSLSNMIKHIGMGLRYLQWWYRKTHVEKKTPFIDMINSVPLR--QIYGCPLGGIGGGTI 164

Query: 115 SRGFRGEFRQWQIIPGTCEASPVMANQFSIFISRDGGNKKYASVLAPGQHEGIGKAGDSG 174
           +RG+RG+F +WQ+ PG  +   V+A+QF++ + R+ G   Y  VL+  +         S 
Sbjct: 165 TRGWRGQFCRWQLNPGMYQHRTVIADQFTVCLRRE-GQTVYQQVLSLER--------PSV 224

Query: 175 ISSWGWNLDGQHSTYHALFPRAWTVYDGEPDPELKVSCRQISPFIPHNYRDSSLPTAVFV 234
           + SW W L G  + YHAL+PRAWTVY   P   + ++CRQI+P +PH+Y+DSSLP  VFV
Sbjct: 225 LRSWNWGLCGYFAFYHALYPRAWTVYQ-LPGQNVTLTCRQITPILPHDYQDSSLPVGVFV 284

Query: 235 YTLVNTGKERAKVSLVFSWANSIGGISHLSGDHVNEPFIGE---DGVSGVLLHHKTAKGN 294
           + + N G E   VS++FS  N +GG     G   NEPF  E   + V G+LLHH T    
Sbjct: 285 WDVENEGDEALDVSIMFSMRNGLGGGDDAPGGLWNEPFCLERSGETVRGLLLHHPTLPN- 344

Query: 295 PPVTFAIAACETQNVSVTVLPSFGLSEGSGVTAKDMWDKMVQDGQFDRGNFSSGPSVPSS 354
            P T A+AA  T   +VT + +F        T + +W  ++QDGQ D     +G S P+ 
Sbjct: 345 -PYTMAVAARVTAATTVTHITAFDPDS----TGQQVWQDLLQDGQLDS---PTGQSTPTQ 404

Query: 355 PGETLCAAVAASAWVEPHGKCTVAFSLSWSSPKVKF-LKGFSYHRRYTKFYGTSEKAAQT 414
            G  +  AV  S+ + P G+C + FSL+W  P++ F  KG  ++RRYT+F+G    AA  
Sbjct: 405 KGVGIAGAVCVSSKLRPRGQCRLEFSLAWDMPRIMFGAKGQVHYRRYTRFFGQDGDAAPA 464

Query: 415 LAHDALTNYKRWEEEIEKWQRPILMDERLPEWYKFTLFNELYFLVAGGTVWIDSSVVGKK 474
           L+H AL  Y  WEE I  WQ P+L D  LP WYK  LFNELYFL  GGTVW++       
Sbjct: 465 LSHYALCRYAEWEERISAWQSPVLDDRSLPAWYKSALFNELYFLADGGTVWLE------- 524

Query: 475 MANDHDELVARIKEDDIKATEAKVSGRGEEVSRTTTTTTLNDFSGVEFDDEENSTSSSHV 534
                      + ED +           EE+ R                           
Sbjct: 525 -----------VLEDSLP----------EELGRN-------------------------- 584

Query: 535 SEDELMVPLKSGYTDRSYQTYKVMDPGNTEEDVGRFLYLEGVEYVMWCTYDVHFYASFAL 594
                M  L+                  T  D GRF YLEG EY M+ TYDVHFYASFAL
Sbjct: 585 -----MCHLRP-----------------TLRDYGRFGYLEGQEYRMYNTYDVHFYASFAL 644

Query: 595 LELFPKIELNIQRDFAKAVLSEDGRKVKFLADGKYGIRKVRGAVPHDLGTHD--PWNEMN 654
           + L+PK+EL++Q D A A L ED  + ++L  G     K R  +PHD+G  D  PW  +N
Sbjct: 645 IMLWPKLELSLQYDMALATLREDLTRRRYLMSGVMAPVKRRNVIPHDIGDPDDEPWLRVN 704

Query: 655 AYNIHDTSRWKDLNTKFVLQVYRDFAATRDMSFGVDVWPSVRAAIEYMEQFDRDGDGVIE 714
           AY IHDT+ WKDLN KFVLQVYRD+  T D +F  D+WP   A +E   +FD+D DG+IE
Sbjct: 705 AYLIHDTADWKDLNLKFVLQVYRDYYLTGDQNFLKDMWPVCLAVMESEMKFDKDHDGLIE 764

Query: 715 NDGFPDQTYDTWTVHGISAYCGCLWVAALQAAAAMAHELGDQEFAETCKSKFLKAKPVLE 774
           N G+ DQTYD W   G SAYCG LW+AA+     MA   G Q+  +   S   + +   E
Sbjct: 765 NGGYADQTYDGWVTTGPSAYCGGLWLAAVAVMVQMAALCGAQDIQDKFSSILSRGQEAYE 824

Query: 775 AKLWNGSYFNYDSGSSSNSKSIQADQLAGQWYTASSGL----PPLFDDFKIKSALKKIYD 834
             LWNG Y+NYDS S   S+S+ +DQ AGQW+  + GL      +F    +  AL+ I++
Sbjct: 825 RLLWNGRYYNYDSSSRPQSRSVMSDQCAGQWFLKACGLGEGDTEVFPTQHVVRALQTIFE 884

Query: 835 FNVMKIRGGRMGAVNGMHPNGKVDETCMQSREIWTGVTYGVAATMILAGMEEEAFKTAEG 894
            NV    GG MGAVNGM P+G  D++ +QS E+W GV YG+AATMI  G+  E F+TAEG
Sbjct: 885 LNVQAFAGGAMGAVNGMQPHGVPDKSSVQSDEVWVGVVYGLAATMIQEGLTWEGFQTAEG 890

Query: 895 IFLAGWSEEGFGYWFQTPEAWSTDGHYRSLIYMRPLSIWGMQWAL 929
            +   W  E  G  FQTPEA+     +RSL YMRPLSIW MQ AL
Sbjct: 945 CYRTVW--ERLGLAFQTPEAYCQQRVFRSLAYMRPLSIWAMQLAL 890

BLAST of CmaCh08G010320 vs. ExPASy Swiss-Prot
Match: Q69ZF3 (Non-lysosomal glucosylceramidase OS=Mus musculus OX=10090 GN=Gba2 PE=1 SV=2)

HSP 1 Score: 619.0 bits (1595), Expect = 9.2e-176
Identity = 360/885 (40.68%), Postives = 482/885 (54.46%), Query Frame = 0

Query: 55  SVTFVEAIKMVRLGIRLWSYVREEASQGRKAP-IDPFTKEACKPSASQGVPLGGMGSGSI 114
           +++    +K + +G+R   +   +    +K P ID       +     G PLGG+G G+I
Sbjct: 96  NISLSNLVKHLGMGLRYLKWWYRKTHVEKKTPFIDMLNSLPLR--QIYGCPLGGIGGGTI 155

Query: 115 SRGFRGEFRQWQIIPGTCEASPVMANQFSIFISRDGGNKKYASVLAPGQHEGIGKAGDSG 174
           +RG+RG+F +WQ+ PG  +   V+A+QF + + RD G   Y  VL+            + 
Sbjct: 156 TRGWRGQFCRWQLNPGMYQHQTVIADQFIVCLRRD-GRTVYQQVLS--------LELPNV 215

Query: 175 ISSWGWNLDGQHSTYHALFPRAWTVYDGEPDPELKVSCRQISPFIPHNYRDSSLPTAVFV 234
           + SW W L G  + YHAL+PRAWTVY   P   + ++CRQ++P +PH+Y+DSSLP  VFV
Sbjct: 216 LRSWNWGLCGYFAFYHALYPRAWTVYQ-LPGQNVTLTCRQVTPILPHDYQDSSLPVGVFV 275

Query: 235 YTLVNTGKERAKVSLVFSWANSIGGISHLSGDHVNEPFIGEDG---VSGVLLHHKTAKGN 294
           + + N G E   VS+ FS  N +GG    +G   NEPF  E G   V G+LLHH T    
Sbjct: 276 WDVENEGDETLDVSITFSMRNGLGGEDDAAGSLWNEPFRLEQGGTTVQGLLLHHPTPPN- 335

Query: 295 PPVTFAIAACETQNVSVTVLPSFGLSEGSGVTAKDMWDKMVQDGQFDRGNFSSGPSVPSS 354
            P T A+AA  T + +VT   +F   + +G T + +W  ++QDGQ D     +G S P+ 
Sbjct: 336 -PYTMAVAARCTADTTVTHTTAF---DPNG-TGQQVWQDLLQDGQLDS---PAGQSTPTQ 395

Query: 355 PGETLCAAVAASAWVEPHGKCTVAFSLSWSSPKVKF-LKGFSYHRRYTKFYGTSEKAAQT 414
            GE +  AV  S+ + P  +C + FSL+W  PK+ F  K   ++RRYT+F+G+    A  
Sbjct: 396 KGEGIAGAVCVSSKLLPRSRCCLEFSLAWDMPKIMFGAKSQVHYRRYTRFFGSDGDVAPA 455

Query: 415 LAHDALTNYKRWEEEIEKWQRPILMDERLPEWYKFTLFNELYFLVAGGTVWIDSSVVGKK 474
           L+H AL +Y  WE+ I  WQ P+L D  LP WYK  LFNELYFL  GGTVW++       
Sbjct: 456 LSHYALCHYADWEDRISAWQNPVLDDRTLPAWYKSALFNELYFLADGGTVWLE------- 515

Query: 475 MANDHDELVARIKEDDIKATEAKVSGRGEEVSRTTTTTTLNDFSGVEFDDEENSTSSSHV 534
                                                                      V
Sbjct: 516 -----------------------------------------------------------V 575

Query: 535 SEDELMVPLKSGYTDRSYQTYKVMDPGNTEEDVGRFLYLEGVEYVMWCTYDVHFYASFAL 594
             D L  P   G + R  +        +T +D GRF YLEG EY M+ TYDVHFYASFAL
Sbjct: 576 PADSL--PEGLGGSMRQLR--------STLQDYGRFGYLEGQEYRMYNTYDVHFYASFAL 635

Query: 595 LELFPKIELNIQRDFAKAVLSEDGRKVKFLADGKYGIRKVRGAVPHDLGTHD--PWNEMN 654
           + L+PK+EL++Q D A A L ED  + ++L  G     K R  +PHD+G  D  PW  +N
Sbjct: 636 VMLWPKLELSLQYDMALATLKEDLTRRRYLMSGVVAPVKRRNVIPHDIGDPDDEPWLRVN 695

Query: 655 AYNIHDTSRWKDLNTKFVLQVYRDFAATRDMSFGVDVWPSVRAAIEYMEQFDRDGDGVIE 714
           AY IHDT+ WKDLN KFVLQ+YRD+  T D  F  D+WP   A +E   +FD+D DG+IE
Sbjct: 696 AYLIHDTADWKDLNLKFVLQIYRDYYLTGDQGFLEDMWPVCLAVMESEMKFDKDQDGLIE 755

Query: 715 NDGFPDQTYDTWTVHGISAYCGCLWVAALQAAAAMAHELGDQEFAETCKSKFLKAKPVLE 774
           N G+ DQTYD W   G SAYCG LW+AA+     MA   G Q+  E   S   + +   E
Sbjct: 756 NGGYADQTYDAWVTTGPSAYCGGLWLAAVAVMVQMAVLCGAQDVQERFASILCRGREAYE 815

Query: 775 AKLWNGSYFNYDSGSSSNSKSIQADQLAGQWYTASSGL----PPLFDDFKIKSALKKIYD 834
             LWNG Y+NYDS S   S+SI +DQ AGQW+  + GL      +F    +  AL+ I++
Sbjct: 816 RLLWNGRYYNYDSSSHPQSRSIMSDQCAGQWFLRACGLGEGDTEVFPTLHVVRALQTIFE 875

Query: 835 FNVMKIRGGRMGAVNGMHPNGKVDETCMQSREIWTGVTYGVAATMILAGMEEEAFKTAEG 894
            NV    GG MGAVNGMHP+G  D + +QS E+W GV YG+AATMI  G+  E F+TAEG
Sbjct: 876 LNVQAFAGGAMGAVNGMHPHGVPDRSSVQSDEVWVGVVYGLAATMIQEGLTWEGFRTAEG 881

Query: 895 IFLAGWSEEGFGYWFQTPEAWSTDGHYRSLIYMRPLSIWGMQWAL 929
            +   W  E  G  FQTPEA+     +RSL YMRPLSIW MQ AL
Sbjct: 936 CYRTVW--ERLGLAFQTPEAYCQQQVFRSLAYMRPLSIWAMQLAL 881

BLAST of CmaCh08G010320 vs. ExPASy Swiss-Prot
Match: Q7KT91 (Non-lysosomal glucosylceramidase OS=Drosophila melanogaster OX=7227 GN=CG33090 PE=1 SV=1)

HSP 1 Score: 487.3 bits (1253), Expect = 4.2e-136
Identity = 324/969 (33.44%), Postives = 462/969 (47.68%), Query Frame = 0

Query: 60  EAIKMVRLGIRLWSYVREEASQGRKAPIDPFTKEACKPSASQGVPLGGMGSGSISRGFRG 119
           + + MV L  R  +Y  + + +GR+  +D +  E  K     GVP+GG+G G+I RG+ G
Sbjct: 77  QTLPMVPLVCRYAAYYWKVSREGRRVYMDYYYMENGK--QIYGVPIGGIGGGTIGRGYAG 136

Query: 120 EFRQWQIIPGTCEASPVMANQFSIFISRDGGNKKYASVLA-----------PGQHEG--- 179
           EF ++Q+ PG  E + V+ANQF + I    G   + S+L+            G  +G   
Sbjct: 137 EFCRFQMRPGIYEYNVVLANQFIVTIKDPKGCTIFQSLLSKCSTRDKTSDPDGDPDGERT 196

Query: 180 ------IGKAGDSGISSWGWNLDGQHSTYHALFPRAWTVYDGEPDPELKVSCRQISPFIP 239
                         +S+W  N++    +Y  L+PR+WT YD      ++++CRQ+SP IP
Sbjct: 197 KCQLPNCSSRAKQPLSAWHSNIEDTRCSYTGLYPRSWTEYD-LSHYGVRLTCRQVSPVIP 256

Query: 240 HNYRDSSLPTAVFVYTLVNTGKERAKVSLVFSWANSIGGISHLSGDHVNEPFIGEDGVSG 299
           H YR+SSLP AVFV+++ N   +  KVS+ F++ N  G     +        I E    G
Sbjct: 257 HEYRESSLPCAVFVWSVENVCDQERKVSITFTFKNGTGNKKQDAEGGAESQLISEGNAKG 316

Query: 300 VLLHHKTAKGNPPVTFAIAACETQNVSVTVLPSFGLSEGSGVTAKDMWDKMVQDGQFDRG 359
           V +  K ++   P ++ +A      +S+T  P F    G+G   + +W ++ + GQ    
Sbjct: 317 VSIRQKISE--MPCSYNLACRVLPEISITRCPQFD-PAGNG---EQLWAQLKEHGQ---- 376

Query: 360 NFSSGPSVPSSPGETLCAAVAASAWVEPHGKCTVAFSLSWSSPKVKFLKGFSYHRR-YTK 419
             S  P+  +   + +  AV     ++P     + F L+W  PK++F +    H R YTK
Sbjct: 377 -LSEHPTSEALKTKDIGVAVCGQVALKPMASHDLEFVLAWDMPKIQFPRKMQTHTRYYTK 436

Query: 420 FYGTSEKAAQTLAHDALTNYKRWEEEIEKWQRPILMDERLPEWYKFTLFNELYFLVAGGT 479
           ++  S  +   +   AL  Y  WE  I+ WQRPIL DE LP+WYK  +FN+LYF+  GGT
Sbjct: 437 YFDDSGDSGPRICEYALRQYSTWERLIDAWQRPILNDETLPDWYKCAIFNQLYFISDGGT 496

Query: 480 VWID-SSVVGKKMANDHDELVARIKEDDIKATEAKVSGRGEEVSRTTTTTTLNDFSGVEF 539
           +W+   S +GK++A D   L                                        
Sbjct: 497 IWLKCDSSLGKELAYDDPRLA--------------------------------------- 556

Query: 540 DDEENSTSSSHVSEDELMVPLKSGYTDRSYQTYKVMDPGNTEEDVGRFLYLEGVEYVMWC 599
                                                        GRF YLEG EY M+ 
Sbjct: 557 --------------------------------------------YGRFGYLEGHEYRMYN 616

Query: 600 TYDVHFYASFALLELFPKIELNIQRDFAKAVLSEDGRKVKFLADGKYGIRKVRGAVPHDL 659
           TYDVHFYAS AL  L+P +++++Q DF  A+ +E     K L DGK   RKV+  VPHDL
Sbjct: 617 TYDVHFYASPALAHLWPNLQVSLQYDFKDAIAAELNDTRKMLYDGKVMPRKVKNCVPHDL 676

Query: 660 GTHD--PWNEMNAYNIHDTSRWKDLNTKFVLQVYRDFAATRDMS---------------- 719
           G  D  P+  +N YNIHD + WKDLNTKFVLQVYRD+    +++                
Sbjct: 677 GDPDEEPFTLINCYNIHDVNDWKDLNTKFVLQVYRDYYVLNELAQAQSDNASKFSSIEFI 736

Query: 720 --------FGVD---------------------------------------VWPSVRAAI 779
                   +  D                                       ++ S +A +
Sbjct: 737 DKESLYELYSQDNKRKNSADEKQQNRKSASMYINETNGKVYLMDAIGYLKAMYASCKAIM 796

Query: 780 EYMEQFDRDGDGVIENDGFPDQTYDTWTVHGISAYCGCLWVAALQAAAAMAHELGDQEFA 839
           E   ++D+D DG+IEN   PDQTYD+W + G SAYC  LW+AALQA +AMA  L      
Sbjct: 797 ERTIEYDKDNDGLIENTKMPDQTYDSWVMDGPSAYCSGLWLAALQAMSAMATILDQPNDC 856

Query: 840 ETCKSKFLKAKPVLEAKLWNGSYFNYDSGSSSNSKSIQADQLAGQWYTASSGLP-PLFDD 899
              +    K K  LE KLWNGSY+ +D  S S+  +I ADQL G WY  S G    ++  
Sbjct: 857 LRYQDILEKGKRSLEEKLWNGSYYRFDL-SHSHRDTIMADQLCGHWYLKSCGFDYEIYPK 916

Query: 900 FKIKSALKKIYDFNVMKIRGGRMGAVNGMHPN-------GKVDETCMQSREIWTGVTYGV 934
             +++ALK+IYD NVM    G +GA NG   N       G VD + +Q+ E+W GV Y +
Sbjct: 917 ENVRTALKRIYDNNVMGFHEGNIGAANGFIANASEPTKPGHVDNSNIQAEEVWPGVVYAL 945

BLAST of CmaCh08G010320 vs. ExPASy TrEMBL
Match: A0A6J1KIS9 (Non-lysosomal glucosylceramidase OS=Cucurbita maxima OX=3661 GN=LOC111494347 PE=3 SV=1)

HSP 1 Score: 2026.1 bits (5248), Expect = 0.0e+00
Identity = 981/981 (100.00%), Postives = 981/981 (100.00%), Query Frame = 0

Query: 1   MVSGNLFHCRKNSWPPDEYISKSTLQLFDFDSASPPEQAWRRKLNGHANLLKEFSVTFVE 60
           MVSGNLFHCRKNSWPPDEYISKSTLQLFDFDSASPPEQAWRRKLNGHANLLKEFSVTFVE
Sbjct: 1   MVSGNLFHCRKNSWPPDEYISKSTLQLFDFDSASPPEQAWRRKLNGHANLLKEFSVTFVE 60

Query: 61  AIKMVRLGIRLWSYVREEASQGRKAPIDPFTKEACKPSASQGVPLGGMGSGSISRGFRGE 120
           AIKMVRLGIRLWSYVREEASQGRKAPIDPFTKEACKPSASQGVPLGGMGSGSISRGFRGE
Sbjct: 61  AIKMVRLGIRLWSYVREEASQGRKAPIDPFTKEACKPSASQGVPLGGMGSGSISRGFRGE 120

Query: 121 FRQWQIIPGTCEASPVMANQFSIFISRDGGNKKYASVLAPGQHEGIGKAGDSGISSWGWN 180
           FRQWQIIPGTCEASPVMANQFSIFISRDGGNKKYASVLAPGQHEGIGKAGDSGISSWGWN
Sbjct: 121 FRQWQIIPGTCEASPVMANQFSIFISRDGGNKKYASVLAPGQHEGIGKAGDSGISSWGWN 180

Query: 181 LDGQHSTYHALFPRAWTVYDGEPDPELKVSCRQISPFIPHNYRDSSLPTAVFVYTLVNTG 240
           LDGQHSTYHALFPRAWTVYDGEPDPELKVSCRQISPFIPHNYRDSSLPTAVFVYTLVNTG
Sbjct: 181 LDGQHSTYHALFPRAWTVYDGEPDPELKVSCRQISPFIPHNYRDSSLPTAVFVYTLVNTG 240

Query: 241 KERAKVSLVFSWANSIGGISHLSGDHVNEPFIGEDGVSGVLLHHKTAKGNPPVTFAIAAC 300
           KERAKVSLVFSWANSIGGISHLSGDHVNEPFIGEDGVSGVLLHHKTAKGNPPVTFAIAAC
Sbjct: 241 KERAKVSLVFSWANSIGGISHLSGDHVNEPFIGEDGVSGVLLHHKTAKGNPPVTFAIAAC 300

Query: 301 ETQNVSVTVLPSFGLSEGSGVTAKDMWDKMVQDGQFDRGNFSSGPSVPSSPGETLCAAVA 360
           ETQNVSVTVLPSFGLSEGSGVTAKDMWDKMVQDGQFDRGNFSSGPSVPSSPGETLCAAVA
Sbjct: 301 ETQNVSVTVLPSFGLSEGSGVTAKDMWDKMVQDGQFDRGNFSSGPSVPSSPGETLCAAVA 360

Query: 361 ASAWVEPHGKCTVAFSLSWSSPKVKFLKGFSYHRRYTKFYGTSEKAAQTLAHDALTNYKR 420
           ASAWVEPHGKCTVAFSLSWSSPKVKFLKGFSYHRRYTKFYGTSEKAAQTLAHDALTNYKR
Sbjct: 361 ASAWVEPHGKCTVAFSLSWSSPKVKFLKGFSYHRRYTKFYGTSEKAAQTLAHDALTNYKR 420

Query: 421 WEEEIEKWQRPILMDERLPEWYKFTLFNELYFLVAGGTVWIDSSVVGKKMANDHDELVAR 480
           WEEEIEKWQRPILMDERLPEWYKFTLFNELYFLVAGGTVWIDSSVVGKKMANDHDELVAR
Sbjct: 421 WEEEIEKWQRPILMDERLPEWYKFTLFNELYFLVAGGTVWIDSSVVGKKMANDHDELVAR 480

Query: 481 IKEDDIKATEAKVSGRGEEVSRTTTTTTLNDFSGVEFDDEENSTSSSHVSEDELMVPLKS 540
           IKEDDIKATEAKVSGRGEEVSRTTTTTTLNDFSGVEFDDEENSTSSSHVSEDELMVPLKS
Sbjct: 481 IKEDDIKATEAKVSGRGEEVSRTTTTTTLNDFSGVEFDDEENSTSSSHVSEDELMVPLKS 540

Query: 541 GYTDRSYQTYKVMDPGNTEEDVGRFLYLEGVEYVMWCTYDVHFYASFALLELFPKIELNI 600
           GYTDRSYQTYKVMDPGNTEEDVGRFLYLEGVEYVMWCTYDVHFYASFALLELFPKIELNI
Sbjct: 541 GYTDRSYQTYKVMDPGNTEEDVGRFLYLEGVEYVMWCTYDVHFYASFALLELFPKIELNI 600

Query: 601 QRDFAKAVLSEDGRKVKFLADGKYGIRKVRGAVPHDLGTHDPWNEMNAYNIHDTSRWKDL 660
           QRDFAKAVLSEDGRKVKFLADGKYGIRKVRGAVPHDLGTHDPWNEMNAYNIHDTSRWKDL
Sbjct: 601 QRDFAKAVLSEDGRKVKFLADGKYGIRKVRGAVPHDLGTHDPWNEMNAYNIHDTSRWKDL 660

Query: 661 NTKFVLQVYRDFAATRDMSFGVDVWPSVRAAIEYMEQFDRDGDGVIENDGFPDQTYDTWT 720
           NTKFVLQVYRDFAATRDMSFGVDVWPSVRAAIEYMEQFDRDGDGVIENDGFPDQTYDTWT
Sbjct: 661 NTKFVLQVYRDFAATRDMSFGVDVWPSVRAAIEYMEQFDRDGDGVIENDGFPDQTYDTWT 720

Query: 721 VHGISAYCGCLWVAALQAAAAMAHELGDQEFAETCKSKFLKAKPVLEAKLWNGSYFNYDS 780
           VHGISAYCGCLWVAALQAAAAMAHELGDQEFAETCKSKFLKAKPVLEAKLWNGSYFNYDS
Sbjct: 721 VHGISAYCGCLWVAALQAAAAMAHELGDQEFAETCKSKFLKAKPVLEAKLWNGSYFNYDS 780

Query: 781 GSSSNSKSIQADQLAGQWYTASSGLPPLFDDFKIKSALKKIYDFNVMKIRGGRMGAVNGM 840
           GSSSNSKSIQADQLAGQWYTASSGLPPLFDDFKIKSALKKIYDFNVMKIRGGRMGAVNGM
Sbjct: 781 GSSSNSKSIQADQLAGQWYTASSGLPPLFDDFKIKSALKKIYDFNVMKIRGGRMGAVNGM 840

Query: 841 HPNGKVDETCMQSREIWTGVTYGVAATMILAGMEEEAFKTAEGIFLAGWSEEGFGYWFQT 900
           HPNGKVDETCMQSREIWTGVTYGVAATMILAGMEEEAFKTAEGIFLAGWSEEGFGYWFQT
Sbjct: 841 HPNGKVDETCMQSREIWTGVTYGVAATMILAGMEEEAFKTAEGIFLAGWSEEGFGYWFQT 900

Query: 901 PEAWSTDGHYRSLIYMRPLSIWGMQWALSLPKAILEAPKINVMDRIHVSSASTKFFHHET 960
           PEAWSTDGHYRSLIYMRPLSIWGMQWALSLPKAILEAPKINVMDRIHVSSASTKFFHHET
Sbjct: 901 PEAWSTDGHYRSLIYMRPLSIWGMQWALSLPKAILEAPKINVMDRIHVSSASTKFFHHET 960

Query: 961 GVRRIATKAKCFGDSVFNCAC 982
           GVRRIATKAKCFGDSVFNCAC
Sbjct: 961 GVRRIATKAKCFGDSVFNCAC 981

BLAST of CmaCh08G010320 vs. ExPASy TrEMBL
Match: A0A6J1HKE8 (Non-lysosomal glucosylceramidase OS=Cucurbita moschata OX=3662 GN=LOC111464357 PE=3 SV=1)

HSP 1 Score: 2016.9 bits (5224), Expect = 0.0e+00
Identity = 975/981 (99.39%), Postives = 980/981 (99.90%), Query Frame = 0

Query: 1   MVSGNLFHCRKNSWPPDEYISKSTLQLFDFDSASPPEQAWRRKLNGHANLLKEFSVTFVE 60
           MVSGNLFHCRKNSWPPDEYISKSTLQLFDFDSASPPEQAWRRKLNGHANLLKEFSVTFVE
Sbjct: 1   MVSGNLFHCRKNSWPPDEYISKSTLQLFDFDSASPPEQAWRRKLNGHANLLKEFSVTFVE 60

Query: 61  AIKMVRLGIRLWSYVREEASQGRKAPIDPFTKEACKPSASQGVPLGGMGSGSISRGFRGE 120
           AIKMVRLGIRLWSYVREEASQGRKAPIDPFTKEACKPSASQGVPLGGMGSGSISRGFRGE
Sbjct: 61  AIKMVRLGIRLWSYVREEASQGRKAPIDPFTKEACKPSASQGVPLGGMGSGSISRGFRGE 120

Query: 121 FRQWQIIPGTCEASPVMANQFSIFISRDGGNKKYASVLAPGQHEGIGKAGDSGISSWGWN 180
           FRQWQIIPGTCEASPVMANQFSIFISRDGGNKKYASVLAPGQHEGIGKAGDSGISSWGWN
Sbjct: 121 FRQWQIIPGTCEASPVMANQFSIFISRDGGNKKYASVLAPGQHEGIGKAGDSGISSWGWN 180

Query: 181 LDGQHSTYHALFPRAWTVYDGEPDPELKVSCRQISPFIPHNYRDSSLPTAVFVYTLVNTG 240
           LDGQHSTYHALFPRAWTVYDGEPDPELKVSCRQISPFIPHNYRDSSLPTAVFVYTLVNTG
Sbjct: 181 LDGQHSTYHALFPRAWTVYDGEPDPELKVSCRQISPFIPHNYRDSSLPTAVFVYTLVNTG 240

Query: 241 KERAKVSLVFSWANSIGGISHLSGDHVNEPFIGEDGVSGVLLHHKTAKGNPPVTFAIAAC 300
           +ERAKVSLVFSWANSIGGISHLSGDHVNEPFIGEDGVSGVLLHHKTAKGNPPVTFAIAAC
Sbjct: 241 RERAKVSLVFSWANSIGGISHLSGDHVNEPFIGEDGVSGVLLHHKTAKGNPPVTFAIAAC 300

Query: 301 ETQNVSVTVLPSFGLSEGSGVTAKDMWDKMVQDGQFDRGNFSSGPSVPSSPGETLCAAVA 360
           ETQNVSVTVLPSFGLSEGSGVTAKDMWDKMVQDGQFDRGN+SSGPSVPSSPGETLCAAVA
Sbjct: 301 ETQNVSVTVLPSFGLSEGSGVTAKDMWDKMVQDGQFDRGNYSSGPSVPSSPGETLCAAVA 360

Query: 361 ASAWVEPHGKCTVAFSLSWSSPKVKFLKGFSYHRRYTKFYGTSEKAAQTLAHDALTNYKR 420
           ASAWVEPHGKCTVAFSLSWSSPKVKFLKGFSYHRRYTKFYGTSEKAAQTLAHDALTNYKR
Sbjct: 361 ASAWVEPHGKCTVAFSLSWSSPKVKFLKGFSYHRRYTKFYGTSEKAAQTLAHDALTNYKR 420

Query: 421 WEEEIEKWQRPILMDERLPEWYKFTLFNELYFLVAGGTVWIDSSVVGKKMANDHDELVAR 480
           WEEEIEKWQ PILMDERLPEWYKFTLFNELYFLVAGGTVWIDSSVVGKKMANDHDELVAR
Sbjct: 421 WEEEIEKWQGPILMDERLPEWYKFTLFNELYFLVAGGTVWIDSSVVGKKMANDHDELVAR 480

Query: 481 IKEDDIKATEAKVSGRGEEVSRTTTTTTLNDFSGVEFDDEENSTSSSHVSEDELMVPLKS 540
           IKE+DIKATEA+VSGRGEEVSRTTTTTTLNDFSGVEFDDEENSTSSSHVSEDELMVPLKS
Sbjct: 481 IKEEDIKATEAEVSGRGEEVSRTTTTTTLNDFSGVEFDDEENSTSSSHVSEDELMVPLKS 540

Query: 541 GYTDRSYQTYKVMDPGNTEEDVGRFLYLEGVEYVMWCTYDVHFYASFALLELFPKIELNI 600
           GYTDRSYQTYKVMDPGNTEEDVGRFLYLEGVEYVMWCTYDVHFYASFALLELFPKIELNI
Sbjct: 541 GYTDRSYQTYKVMDPGNTEEDVGRFLYLEGVEYVMWCTYDVHFYASFALLELFPKIELNI 600

Query: 601 QRDFAKAVLSEDGRKVKFLADGKYGIRKVRGAVPHDLGTHDPWNEMNAYNIHDTSRWKDL 660
           QRDFAKAVLSEDGRKVKFLADGKYGIRKVRGAVPHDLGTHDPWNEMNAYNIHDTSRWKDL
Sbjct: 601 QRDFAKAVLSEDGRKVKFLADGKYGIRKVRGAVPHDLGTHDPWNEMNAYNIHDTSRWKDL 660

Query: 661 NTKFVLQVYRDFAATRDMSFGVDVWPSVRAAIEYMEQFDRDGDGVIENDGFPDQTYDTWT 720
           NTKFVLQVYRDFAATRDMSFGVDVWPSVRAAIEYMEQFDRDGDGVIENDGFPDQTYDTWT
Sbjct: 661 NTKFVLQVYRDFAATRDMSFGVDVWPSVRAAIEYMEQFDRDGDGVIENDGFPDQTYDTWT 720

Query: 721 VHGISAYCGCLWVAALQAAAAMAHELGDQEFAETCKSKFLKAKPVLEAKLWNGSYFNYDS 780
           VHGISAYCGCLWVAALQAAAAMAHELGDQEFAETCKSKFLKAKPVLEAKLWNGSYFNYDS
Sbjct: 721 VHGISAYCGCLWVAALQAAAAMAHELGDQEFAETCKSKFLKAKPVLEAKLWNGSYFNYDS 780

Query: 781 GSSSNSKSIQADQLAGQWYTASSGLPPLFDDFKIKSALKKIYDFNVMKIRGGRMGAVNGM 840
           GSSSNSKSIQADQLAGQWYTASSGLPPLFDDFKIKSALKKIYDFNVMKI+GGRMGAVNGM
Sbjct: 781 GSSSNSKSIQADQLAGQWYTASSGLPPLFDDFKIKSALKKIYDFNVMKIKGGRMGAVNGM 840

Query: 841 HPNGKVDETCMQSREIWTGVTYGVAATMILAGMEEEAFKTAEGIFLAGWSEEGFGYWFQT 900
           HPNGKVDETCMQSREIWTGVTYGVAATMILAGMEEEAFKTAEGIFLAGWSEEGFGYWFQT
Sbjct: 841 HPNGKVDETCMQSREIWTGVTYGVAATMILAGMEEEAFKTAEGIFLAGWSEEGFGYWFQT 900

Query: 901 PEAWSTDGHYRSLIYMRPLSIWGMQWALSLPKAILEAPKINVMDRIHVSSASTKFFHHET 960
           PEAWSTDGHYRSLIYMRPLSIWGMQWALSLPKAILEAPKINVMDRIHVSSASTKFFHHET
Sbjct: 901 PEAWSTDGHYRSLIYMRPLSIWGMQWALSLPKAILEAPKINVMDRIHVSSASTKFFHHET 960

Query: 961 GVRRIATKAKCFGDSVFNCAC 982
           GVRRIATKAKCFGDSVFNCAC
Sbjct: 961 GVRRIATKAKCFGDSVFNCAC 981

BLAST of CmaCh08G010320 vs. ExPASy TrEMBL
Match: A0A0A0LK88 (Non-lysosomal glucosylceramidase OS=Cucumis sativus OX=3659 GN=Csa_2G130670 PE=3 SV=1)

HSP 1 Score: 1932.5 bits (5005), Expect = 0.0e+00
Identity = 928/981 (94.60%), Postives = 960/981 (97.86%), Query Frame = 0

Query: 1   MVSGNLFHCRKNSWPPDEYISKSTLQLFDFDSASPPEQAWRRKLNGHANLLKEFSVTFVE 60
           MVSGNLFHCRKNSWPP+EYISKSTLQLFDFDSASPPEQAWRRKLNGHANLLKEFSVTFVE
Sbjct: 1   MVSGNLFHCRKNSWPPEEYISKSTLQLFDFDSASPPEQAWRRKLNGHANLLKEFSVTFVE 60

Query: 61  AIKMVRLGIRLWSYVREEASQGRKAPIDPFTKEACKPSASQGVPLGGMGSGSISRGFRGE 120
           AIKMVRLGIRLWSYVREEASQGRKAPIDPFT+E+CKPSASQGVPLGGMGSGSISRGFRGE
Sbjct: 61  AIKMVRLGIRLWSYVREEASQGRKAPIDPFTRESCKPSASQGVPLGGMGSGSISRGFRGE 120

Query: 121 FRQWQIIPGTCEASPVMANQFSIFISRDGGNKKYASVLAPGQHEGIGKAGDSGISSWGWN 180
           FRQWQIIPGTCEASPVMANQFSIF+SRDGG KKYASVLAPGQHEG+GK GDSGISSWGWN
Sbjct: 121 FRQWQIIPGTCEASPVMANQFSIFVSRDGGGKKYASVLAPGQHEGLGKDGDSGISSWGWN 180

Query: 181 LDGQHSTYHALFPRAWTVYDGEPDPELKVSCRQISPFIPHNYRDSSLPTAVFVYTLVNTG 240
           LDGQHSTYHALFPRAWTVYDGEPDPELKVSCRQISPFIPHNYRDSSLPTAVFVYTLVNTG
Sbjct: 181 LDGQHSTYHALFPRAWTVYDGEPDPELKVSCRQISPFIPHNYRDSSLPTAVFVYTLVNTG 240

Query: 241 KERAKVSLVFSWANSIGGISHLSGDHVNEPFIGEDGVSGVLLHHKTAKGNPPVTFAIAAC 300
           +ERAKVSL+F+WANSIGG SHLSG+HVNEPFI EDGVSGVLLHHKTAKGNPPVTFAIAAC
Sbjct: 241 RERAKVSLLFTWANSIGGNSHLSGNHVNEPFIDEDGVSGVLLHHKTAKGNPPVTFAIAAC 300

Query: 301 ETQNVSVTVLPSFGLSEGSGVTAKDMWDKMVQDGQFDRGNFSSGPSVPSSPGETLCAAVA 360
           ETQNVSVTVLPSFGLSEGS +TAKDMWDKMVQDGQFDR NFSSGPS+PSSPGETLCAAVA
Sbjct: 301 ETQNVSVTVLPSFGLSEGSCITAKDMWDKMVQDGQFDRDNFSSGPSMPSSPGETLCAAVA 360

Query: 361 ASAWVEPHGKCTVAFSLSWSSPKVKFLKGFSYHRRYTKFYGTSEKAAQTLAHDALTNYKR 420
           ASAWVEPHGKCTVAFSLSWSSPKVKFLKGFSYHRRYTKFYGTS KAAQTLAHDALTNYKR
Sbjct: 361 ASAWVEPHGKCTVAFSLSWSSPKVKFLKGFSYHRRYTKFYGTSGKAAQTLAHDALTNYKR 420

Query: 421 WEEEIEKWQRPILMDERLPEWYKFTLFNELYFLVAGGTVWIDSSVVGKKMANDHDELVAR 480
           WEEEIEKWQRP+LMDERLPEWYKFTLFNELYFLVAGGTVWIDSS VGKK + D D L AR
Sbjct: 421 WEEEIEKWQRPVLMDERLPEWYKFTLFNELYFLVAGGTVWIDSSFVGKKASYDQDHL-AR 480

Query: 481 IKEDDIKATEAKVSGRGEEVSRTTTTTTLNDFSGVEFDDEENSTSSSHVSEDELMVPLKS 540
           +K DD+KA EAKVSGRGEEVSRTTTTTTL+ F  +E+DD ENSTSSSH SEDELMVPLK 
Sbjct: 481 LKNDDVKAVEAKVSGRGEEVSRTTTTTTLDGFPSIEYDD-ENSTSSSHASEDELMVPLKR 540

Query: 541 GYTDRSYQTYKVMDPGNTEEDVGRFLYLEGVEYVMWCTYDVHFYASFALLELFPKIELNI 600
           GYTDRSYQTYKV++PGNTEEDVGRFLYLEGVEYVMWCTYDVHFYAS+ALLELFPKIELNI
Sbjct: 541 GYTDRSYQTYKVLEPGNTEEDVGRFLYLEGVEYVMWCTYDVHFYASYALLELFPKIELNI 600

Query: 601 QRDFAKAVLSEDGRKVKFLADGKYGIRKVRGAVPHDLGTHDPWNEMNAYNIHDTSRWKDL 660
           QRDFAKAVLSEDGRKV+FLA+GKYGIRKVRGAVPHDLGTHDPWNEMNAYNIHDTSRWKDL
Sbjct: 601 QRDFAKAVLSEDGRKVRFLAEGKYGIRKVRGAVPHDLGTHDPWNEMNAYNIHDTSRWKDL 660

Query: 661 NTKFVLQVYRDFAATRDMSFGVDVWPSVRAAIEYMEQFDRDGDGVIENDGFPDQTYDTWT 720
           NTKFVLQVYRDFAATRDMSFGVDVWPSVRAAIEYMEQFDRDGDGVIENDGFPDQTYDTWT
Sbjct: 661 NTKFVLQVYRDFAATRDMSFGVDVWPSVRAAIEYMEQFDRDGDGVIENDGFPDQTYDTWT 720

Query: 721 VHGISAYCGCLWVAALQAAAAMAHELGDQEFAETCKSKFLKAKPVLEAKLWNGSYFNYDS 780
           VHGISAYCGCLWVAALQAAAAMAHELGDQEFAETCKSKFLKA+PVLEA+LWNGSYFNYDS
Sbjct: 721 VHGISAYCGCLWVAALQAAAAMAHELGDQEFAETCKSKFLKARPVLEAELWNGSYFNYDS 780

Query: 781 GSSSNSKSIQADQLAGQWYTASSGLPPLFDDFKIKSALKKIYDFNVMKIRGGRMGAVNGM 840
           GSSSNSKSIQADQLAGQWYTASSGLPPLFDDFKIKSAL+KIYDFNVMK+RGGRMGAVNGM
Sbjct: 781 GSSSNSKSIQADQLAGQWYTASSGLPPLFDDFKIKSALRKIYDFNVMKVRGGRMGAVNGM 840

Query: 841 HPNGKVDETCMQSREIWTGVTYGVAATMILAGMEEEAFKTAEGIFLAGWSEEGFGYWFQT 900
           HPNGK+DETCMQSREIWTGVTYGVAATMILAGMEEEAFKTAEGIFLAGWSEEGFGYWFQT
Sbjct: 841 HPNGKIDETCMQSREIWTGVTYGVAATMILAGMEEEAFKTAEGIFLAGWSEEGFGYWFQT 900

Query: 901 PEAWSTDGHYRSLIYMRPLSIWGMQWALSLPKAILEAPKINVMDRIHVSSASTKFFHHET 960
           PEAWSTDGHYRSLIYMRPLSIWGMQWALSLPKAIL+APKINVMDRIHVSS++TKFF+HET
Sbjct: 901 PEAWSTDGHYRSLIYMRPLSIWGMQWALSLPKAILDAPKINVMDRIHVSSSNTKFFNHET 960

Query: 961 GVRRIATKAKCFGDSVFNCAC 982
           GVRRIATKAKCFGDSVFNCAC
Sbjct: 961 GVRRIATKAKCFGDSVFNCAC 979

BLAST of CmaCh08G010320 vs. ExPASy TrEMBL
Match: A0A5D3CTQ8 (Non-lysosomal glucosylceramidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold472G00270 PE=3 SV=1)

HSP 1 Score: 1930.6 bits (5000), Expect = 0.0e+00
Identity = 927/981 (94.50%), Postives = 961/981 (97.96%), Query Frame = 0

Query: 1   MVSGNLFHCRKNSWPPDEYISKSTLQLFDFDSASPPEQAWRRKLNGHANLLKEFSVTFVE 60
           MVSGNLFHCRKNSWPP+EYISKSTLQLFDFDSASPPEQAWRRKLNGHANLLKEFSVTFVE
Sbjct: 1   MVSGNLFHCRKNSWPPEEYISKSTLQLFDFDSASPPEQAWRRKLNGHANLLKEFSVTFVE 60

Query: 61  AIKMVRLGIRLWSYVREEASQGRKAPIDPFTKEACKPSASQGVPLGGMGSGSISRGFRGE 120
           AIKMVRLGIRLWSYVREEASQGRKAPIDPFT+E+CKPSASQGVPLGGMGSGSISRGFRGE
Sbjct: 61  AIKMVRLGIRLWSYVREEASQGRKAPIDPFTRESCKPSASQGVPLGGMGSGSISRGFRGE 120

Query: 121 FRQWQIIPGTCEASPVMANQFSIFISRDGGNKKYASVLAPGQHEGIGKAGDSGISSWGWN 180
           FRQWQIIPGTCEASP+MANQFSIF+SRDGG+KKYASVLAPGQHEG+GKAGDSGISSWGWN
Sbjct: 121 FRQWQIIPGTCEASPIMANQFSIFVSRDGGSKKYASVLAPGQHEGLGKAGDSGISSWGWN 180

Query: 181 LDGQHSTYHALFPRAWTVYDGEPDPELKVSCRQISPFIPHNYRDSSLPTAVFVYTLVNTG 240
           LDGQHSTYHALFPRAWTVYDGEPDPELKVSCRQISPFIPHNYRDSSLPTAVFVYTLVNTG
Sbjct: 181 LDGQHSTYHALFPRAWTVYDGEPDPELKVSCRQISPFIPHNYRDSSLPTAVFVYTLVNTG 240

Query: 241 KERAKVSLVFSWANSIGGISHLSGDHVNEPFIGEDGVSGVLLHHKTAKGNPPVTFAIAAC 300
           +ERAKVSL+F+WANSIGGISHLSG+HVNEPFI EDGVSGVLLHHKTAKGNPPVTFAIAAC
Sbjct: 241 RERAKVSLLFTWANSIGGISHLSGNHVNEPFIDEDGVSGVLLHHKTAKGNPPVTFAIAAC 300

Query: 301 ETQNVSVTVLPSFGLSEGSGVTAKDMWDKMVQDGQFDRGNFSSGPSVPSSPGETLCAAVA 360
           ETQNVSVTVLPSFGLSEGS VTAKDMWDKMVQDGQFDR NFSSGPS+PSSPGETLCAAVA
Sbjct: 301 ETQNVSVTVLPSFGLSEGSCVTAKDMWDKMVQDGQFDRDNFSSGPSMPSSPGETLCAAVA 360

Query: 361 ASAWVEPHGKCTVAFSLSWSSPKVKFLKGFSYHRRYTKFYGTSEKAAQTLAHDALTNYKR 420
           ASAWVEPHGKCTVAFSLSWSSPKVKFLKGFSYHRRYTKFYGTS KAAQTLAHDALTNYKR
Sbjct: 361 ASAWVEPHGKCTVAFSLSWSSPKVKFLKGFSYHRRYTKFYGTSGKAAQTLAHDALTNYKR 420

Query: 421 WEEEIEKWQRPILMDERLPEWYKFTLFNELYFLVAGGTVWIDSSVVGKKMANDHDELVAR 480
           WEEEIEKWQ P+LMDERLPEWYKFTLFNELYFLVAGGTVWIDSS VGKK   D D+L AR
Sbjct: 421 WEEEIEKWQMPVLMDERLPEWYKFTLFNELYFLVAGGTVWIDSSFVGKKAPYDQDQL-AR 480

Query: 481 IKEDDIKATEAKVSGRGEEVSRTTTTTTLNDFSGVEFDDEENSTSSSHVSEDELMVPLKS 540
           +K D++KA EA VSGRGEEVSRT TT TL++F G+E+DD ENSTSSSH SEDELMVPLK 
Sbjct: 481 MKNDEVKAVEATVSGRGEEVSRTITTATLDEFPGIEYDD-ENSTSSSHASEDELMVPLKR 540

Query: 541 GYTDRSYQTYKVMDPGNTEEDVGRFLYLEGVEYVMWCTYDVHFYASFALLELFPKIELNI 600
           GYTDRSYQTYKV++PGNTEEDVGRFLYLEGVEYVMWCTYDVHFYAS+ALLELFPKIELNI
Sbjct: 541 GYTDRSYQTYKVLEPGNTEEDVGRFLYLEGVEYVMWCTYDVHFYASYALLELFPKIELNI 600

Query: 601 QRDFAKAVLSEDGRKVKFLADGKYGIRKVRGAVPHDLGTHDPWNEMNAYNIHDTSRWKDL 660
           QRDFAKAVLSEDGRKVKFLADGK+GIRKVRGAVPHDLGTHDPWNEMNAYNIHDTSRWKDL
Sbjct: 601 QRDFAKAVLSEDGRKVKFLADGKFGIRKVRGAVPHDLGTHDPWNEMNAYNIHDTSRWKDL 660

Query: 661 NTKFVLQVYRDFAATRDMSFGVDVWPSVRAAIEYMEQFDRDGDGVIENDGFPDQTYDTWT 720
           NTKFVLQVYRDFAATRDMSFGVDVWPSVRAAIEYMEQFDRDGDGVIENDGFPDQTYDTWT
Sbjct: 661 NTKFVLQVYRDFAATRDMSFGVDVWPSVRAAIEYMEQFDRDGDGVIENDGFPDQTYDTWT 720

Query: 721 VHGISAYCGCLWVAALQAAAAMAHELGDQEFAETCKSKFLKAKPVLEAKLWNGSYFNYDS 780
           VHGISAYCGCLWVAALQAAAAMAHELGD+EFAETCKSKFLKA+PVLEA+LWNGSYFNYDS
Sbjct: 721 VHGISAYCGCLWVAALQAAAAMAHELGDREFAETCKSKFLKARPVLEAELWNGSYFNYDS 780

Query: 781 GSSSNSKSIQADQLAGQWYTASSGLPPLFDDFKIKSALKKIYDFNVMKIRGGRMGAVNGM 840
           GSSSNSKSIQADQLAGQWYTASSGLPPLFDDFKIKSAL+KIYDFNVMK+RGGRMGAVNGM
Sbjct: 781 GSSSNSKSIQADQLAGQWYTASSGLPPLFDDFKIKSALRKIYDFNVMKVRGGRMGAVNGM 840

Query: 841 HPNGKVDETCMQSREIWTGVTYGVAATMILAGMEEEAFKTAEGIFLAGWSEEGFGYWFQT 900
           HPNGKVDETCMQSREIWTGVTYGVAATMILAGMEEEAFKTAEGIFLAGWSEEGFGYWFQT
Sbjct: 841 HPNGKVDETCMQSREIWTGVTYGVAATMILAGMEEEAFKTAEGIFLAGWSEEGFGYWFQT 900

Query: 901 PEAWSTDGHYRSLIYMRPLSIWGMQWALSLPKAILEAPKINVMDRIHVSSASTKFFHHET 960
           PEAWSTDGHYRSLIYMRPLSIWGMQWALSLPKAIL+APKINVMDRIHVSS++TKFF+HET
Sbjct: 901 PEAWSTDGHYRSLIYMRPLSIWGMQWALSLPKAILDAPKINVMDRIHVSSSNTKFFNHET 960

Query: 961 GVRRIATKAKCFGDSVFNCAC 982
           GVRRIATKAKCFGDSVFNCAC
Sbjct: 961 GVRRIATKAKCFGDSVFNCAC 979

BLAST of CmaCh08G010320 vs. ExPASy TrEMBL
Match: A0A1S3C4N3 (Non-lysosomal glucosylceramidase OS=Cucumis melo OX=3656 GN=LOC103496976 PE=3 SV=1)

HSP 1 Score: 1930.6 bits (5000), Expect = 0.0e+00
Identity = 927/981 (94.50%), Postives = 961/981 (97.96%), Query Frame = 0

Query: 1   MVSGNLFHCRKNSWPPDEYISKSTLQLFDFDSASPPEQAWRRKLNGHANLLKEFSVTFVE 60
           MVSGNLFHCRKNSWPP+EYISKSTLQLFDFDSASPPEQAWRRKLNGHANLLKEFSVTFVE
Sbjct: 1   MVSGNLFHCRKNSWPPEEYISKSTLQLFDFDSASPPEQAWRRKLNGHANLLKEFSVTFVE 60

Query: 61  AIKMVRLGIRLWSYVREEASQGRKAPIDPFTKEACKPSASQGVPLGGMGSGSISRGFRGE 120
           AIKMVRLGIRLWSYVREEASQGRKAPIDPFT+E+CKPSASQGVPLGGMGSGSISRGFRGE
Sbjct: 61  AIKMVRLGIRLWSYVREEASQGRKAPIDPFTRESCKPSASQGVPLGGMGSGSISRGFRGE 120

Query: 121 FRQWQIIPGTCEASPVMANQFSIFISRDGGNKKYASVLAPGQHEGIGKAGDSGISSWGWN 180
           FRQWQIIPGTCEASP+MANQFSIF+SRDGG+KKYASVLAPGQHEG+GKAGDSGISSWGWN
Sbjct: 121 FRQWQIIPGTCEASPIMANQFSIFVSRDGGSKKYASVLAPGQHEGLGKAGDSGISSWGWN 180

Query: 181 LDGQHSTYHALFPRAWTVYDGEPDPELKVSCRQISPFIPHNYRDSSLPTAVFVYTLVNTG 240
           LDGQHSTYHALFPRAWTVYDGEPDPELKVSCRQISPFIPHNYRDSSLPTAVFVYTLVNTG
Sbjct: 181 LDGQHSTYHALFPRAWTVYDGEPDPELKVSCRQISPFIPHNYRDSSLPTAVFVYTLVNTG 240

Query: 241 KERAKVSLVFSWANSIGGISHLSGDHVNEPFIGEDGVSGVLLHHKTAKGNPPVTFAIAAC 300
           +ERAKVSL+F+WANSIGGISHLSG+HVNEPFI EDGVSGVLLHHKTAKGNPPVTFAIAAC
Sbjct: 241 RERAKVSLLFTWANSIGGISHLSGNHVNEPFIDEDGVSGVLLHHKTAKGNPPVTFAIAAC 300

Query: 301 ETQNVSVTVLPSFGLSEGSGVTAKDMWDKMVQDGQFDRGNFSSGPSVPSSPGETLCAAVA 360
           ETQNVSVTVLPSFGLSEGS VTAKDMWDKMVQDGQFDR NFSSGPS+PSSPGETLCAAVA
Sbjct: 301 ETQNVSVTVLPSFGLSEGSCVTAKDMWDKMVQDGQFDRDNFSSGPSMPSSPGETLCAAVA 360

Query: 361 ASAWVEPHGKCTVAFSLSWSSPKVKFLKGFSYHRRYTKFYGTSEKAAQTLAHDALTNYKR 420
           ASAWVEPHGKCTVAFSLSWSSPKVKFLKGFSYHRRYTKFYGTS KAAQTLAHDALTNYKR
Sbjct: 361 ASAWVEPHGKCTVAFSLSWSSPKVKFLKGFSYHRRYTKFYGTSGKAAQTLAHDALTNYKR 420

Query: 421 WEEEIEKWQRPILMDERLPEWYKFTLFNELYFLVAGGTVWIDSSVVGKKMANDHDELVAR 480
           WEEEIEKWQ P+LMDERLPEWYKFTLFNELYFLVAGGTVWIDSS VGKK   D D+L AR
Sbjct: 421 WEEEIEKWQMPVLMDERLPEWYKFTLFNELYFLVAGGTVWIDSSFVGKKAPYDQDQL-AR 480

Query: 481 IKEDDIKATEAKVSGRGEEVSRTTTTTTLNDFSGVEFDDEENSTSSSHVSEDELMVPLKS 540
           +K D++KA EA VSGRGEEVSRT TT TL++F G+E+DD ENSTSSSH SEDELMVPLK 
Sbjct: 481 MKNDEVKAVEATVSGRGEEVSRTITTATLDEFPGIEYDD-ENSTSSSHASEDELMVPLKR 540

Query: 541 GYTDRSYQTYKVMDPGNTEEDVGRFLYLEGVEYVMWCTYDVHFYASFALLELFPKIELNI 600
           GYTDRSYQTYKV++PGNTEEDVGRFLYLEGVEYVMWCTYDVHFYAS+ALLELFPKIELNI
Sbjct: 541 GYTDRSYQTYKVLEPGNTEEDVGRFLYLEGVEYVMWCTYDVHFYASYALLELFPKIELNI 600

Query: 601 QRDFAKAVLSEDGRKVKFLADGKYGIRKVRGAVPHDLGTHDPWNEMNAYNIHDTSRWKDL 660
           QRDFAKAVLSEDGRKVKFLADGK+GIRKVRGAVPHDLGTHDPWNEMNAYNIHDTSRWKDL
Sbjct: 601 QRDFAKAVLSEDGRKVKFLADGKFGIRKVRGAVPHDLGTHDPWNEMNAYNIHDTSRWKDL 660

Query: 661 NTKFVLQVYRDFAATRDMSFGVDVWPSVRAAIEYMEQFDRDGDGVIENDGFPDQTYDTWT 720
           NTKFVLQVYRDFAATRDMSFGVDVWPSVRAAIEYMEQFDRDGDGVIENDGFPDQTYDTWT
Sbjct: 661 NTKFVLQVYRDFAATRDMSFGVDVWPSVRAAIEYMEQFDRDGDGVIENDGFPDQTYDTWT 720

Query: 721 VHGISAYCGCLWVAALQAAAAMAHELGDQEFAETCKSKFLKAKPVLEAKLWNGSYFNYDS 780
           VHGISAYCGCLWVAALQAAAAMAHELGD+EFAETCKSKFLKA+PVLEA+LWNGSYFNYDS
Sbjct: 721 VHGISAYCGCLWVAALQAAAAMAHELGDREFAETCKSKFLKARPVLEAELWNGSYFNYDS 780

Query: 781 GSSSNSKSIQADQLAGQWYTASSGLPPLFDDFKIKSALKKIYDFNVMKIRGGRMGAVNGM 840
           GSSSNSKSIQADQLAGQWYTASSGLPPLFDDFKIKSAL+KIYDFNVMK+RGGRMGAVNGM
Sbjct: 781 GSSSNSKSIQADQLAGQWYTASSGLPPLFDDFKIKSALRKIYDFNVMKVRGGRMGAVNGM 840

Query: 841 HPNGKVDETCMQSREIWTGVTYGVAATMILAGMEEEAFKTAEGIFLAGWSEEGFGYWFQT 900
           HPNGKVDETCMQSREIWTGVTYGVAATMILAGMEEEAFKTAEGIFLAGWSEEGFGYWFQT
Sbjct: 841 HPNGKVDETCMQSREIWTGVTYGVAATMILAGMEEEAFKTAEGIFLAGWSEEGFGYWFQT 900

Query: 901 PEAWSTDGHYRSLIYMRPLSIWGMQWALSLPKAILEAPKINVMDRIHVSSASTKFFHHET 960
           PEAWSTDGHYRSLIYMRPLSIWGMQWALSLPKAIL+APKINVMDRIHVSS++TKFF+HET
Sbjct: 901 PEAWSTDGHYRSLIYMRPLSIWGMQWALSLPKAILDAPKINVMDRIHVSSSNTKFFNHET 960

Query: 961 GVRRIATKAKCFGDSVFNCAC 982
           GVRRIATKAKCFGDSVFNCAC
Sbjct: 961 GVRRIATKAKCFGDSVFNCAC 979

BLAST of CmaCh08G010320 vs. NCBI nr
Match: XP_023000039.1 (non-lysosomal glucosylceramidase [Cucurbita maxima])

HSP 1 Score: 2026.1 bits (5248), Expect = 0.0e+00
Identity = 981/981 (100.00%), Postives = 981/981 (100.00%), Query Frame = 0

Query: 1   MVSGNLFHCRKNSWPPDEYISKSTLQLFDFDSASPPEQAWRRKLNGHANLLKEFSVTFVE 60
           MVSGNLFHCRKNSWPPDEYISKSTLQLFDFDSASPPEQAWRRKLNGHANLLKEFSVTFVE
Sbjct: 1   MVSGNLFHCRKNSWPPDEYISKSTLQLFDFDSASPPEQAWRRKLNGHANLLKEFSVTFVE 60

Query: 61  AIKMVRLGIRLWSYVREEASQGRKAPIDPFTKEACKPSASQGVPLGGMGSGSISRGFRGE 120
           AIKMVRLGIRLWSYVREEASQGRKAPIDPFTKEACKPSASQGVPLGGMGSGSISRGFRGE
Sbjct: 61  AIKMVRLGIRLWSYVREEASQGRKAPIDPFTKEACKPSASQGVPLGGMGSGSISRGFRGE 120

Query: 121 FRQWQIIPGTCEASPVMANQFSIFISRDGGNKKYASVLAPGQHEGIGKAGDSGISSWGWN 180
           FRQWQIIPGTCEASPVMANQFSIFISRDGGNKKYASVLAPGQHEGIGKAGDSGISSWGWN
Sbjct: 121 FRQWQIIPGTCEASPVMANQFSIFISRDGGNKKYASVLAPGQHEGIGKAGDSGISSWGWN 180

Query: 181 LDGQHSTYHALFPRAWTVYDGEPDPELKVSCRQISPFIPHNYRDSSLPTAVFVYTLVNTG 240
           LDGQHSTYHALFPRAWTVYDGEPDPELKVSCRQISPFIPHNYRDSSLPTAVFVYTLVNTG
Sbjct: 181 LDGQHSTYHALFPRAWTVYDGEPDPELKVSCRQISPFIPHNYRDSSLPTAVFVYTLVNTG 240

Query: 241 KERAKVSLVFSWANSIGGISHLSGDHVNEPFIGEDGVSGVLLHHKTAKGNPPVTFAIAAC 300
           KERAKVSLVFSWANSIGGISHLSGDHVNEPFIGEDGVSGVLLHHKTAKGNPPVTFAIAAC
Sbjct: 241 KERAKVSLVFSWANSIGGISHLSGDHVNEPFIGEDGVSGVLLHHKTAKGNPPVTFAIAAC 300

Query: 301 ETQNVSVTVLPSFGLSEGSGVTAKDMWDKMVQDGQFDRGNFSSGPSVPSSPGETLCAAVA 360
           ETQNVSVTVLPSFGLSEGSGVTAKDMWDKMVQDGQFDRGNFSSGPSVPSSPGETLCAAVA
Sbjct: 301 ETQNVSVTVLPSFGLSEGSGVTAKDMWDKMVQDGQFDRGNFSSGPSVPSSPGETLCAAVA 360

Query: 361 ASAWVEPHGKCTVAFSLSWSSPKVKFLKGFSYHRRYTKFYGTSEKAAQTLAHDALTNYKR 420
           ASAWVEPHGKCTVAFSLSWSSPKVKFLKGFSYHRRYTKFYGTSEKAAQTLAHDALTNYKR
Sbjct: 361 ASAWVEPHGKCTVAFSLSWSSPKVKFLKGFSYHRRYTKFYGTSEKAAQTLAHDALTNYKR 420

Query: 421 WEEEIEKWQRPILMDERLPEWYKFTLFNELYFLVAGGTVWIDSSVVGKKMANDHDELVAR 480
           WEEEIEKWQRPILMDERLPEWYKFTLFNELYFLVAGGTVWIDSSVVGKKMANDHDELVAR
Sbjct: 421 WEEEIEKWQRPILMDERLPEWYKFTLFNELYFLVAGGTVWIDSSVVGKKMANDHDELVAR 480

Query: 481 IKEDDIKATEAKVSGRGEEVSRTTTTTTLNDFSGVEFDDEENSTSSSHVSEDELMVPLKS 540
           IKEDDIKATEAKVSGRGEEVSRTTTTTTLNDFSGVEFDDEENSTSSSHVSEDELMVPLKS
Sbjct: 481 IKEDDIKATEAKVSGRGEEVSRTTTTTTLNDFSGVEFDDEENSTSSSHVSEDELMVPLKS 540

Query: 541 GYTDRSYQTYKVMDPGNTEEDVGRFLYLEGVEYVMWCTYDVHFYASFALLELFPKIELNI 600
           GYTDRSYQTYKVMDPGNTEEDVGRFLYLEGVEYVMWCTYDVHFYASFALLELFPKIELNI
Sbjct: 541 GYTDRSYQTYKVMDPGNTEEDVGRFLYLEGVEYVMWCTYDVHFYASFALLELFPKIELNI 600

Query: 601 QRDFAKAVLSEDGRKVKFLADGKYGIRKVRGAVPHDLGTHDPWNEMNAYNIHDTSRWKDL 660
           QRDFAKAVLSEDGRKVKFLADGKYGIRKVRGAVPHDLGTHDPWNEMNAYNIHDTSRWKDL
Sbjct: 601 QRDFAKAVLSEDGRKVKFLADGKYGIRKVRGAVPHDLGTHDPWNEMNAYNIHDTSRWKDL 660

Query: 661 NTKFVLQVYRDFAATRDMSFGVDVWPSVRAAIEYMEQFDRDGDGVIENDGFPDQTYDTWT 720
           NTKFVLQVYRDFAATRDMSFGVDVWPSVRAAIEYMEQFDRDGDGVIENDGFPDQTYDTWT
Sbjct: 661 NTKFVLQVYRDFAATRDMSFGVDVWPSVRAAIEYMEQFDRDGDGVIENDGFPDQTYDTWT 720

Query: 721 VHGISAYCGCLWVAALQAAAAMAHELGDQEFAETCKSKFLKAKPVLEAKLWNGSYFNYDS 780
           VHGISAYCGCLWVAALQAAAAMAHELGDQEFAETCKSKFLKAKPVLEAKLWNGSYFNYDS
Sbjct: 721 VHGISAYCGCLWVAALQAAAAMAHELGDQEFAETCKSKFLKAKPVLEAKLWNGSYFNYDS 780

Query: 781 GSSSNSKSIQADQLAGQWYTASSGLPPLFDDFKIKSALKKIYDFNVMKIRGGRMGAVNGM 840
           GSSSNSKSIQADQLAGQWYTASSGLPPLFDDFKIKSALKKIYDFNVMKIRGGRMGAVNGM
Sbjct: 781 GSSSNSKSIQADQLAGQWYTASSGLPPLFDDFKIKSALKKIYDFNVMKIRGGRMGAVNGM 840

Query: 841 HPNGKVDETCMQSREIWTGVTYGVAATMILAGMEEEAFKTAEGIFLAGWSEEGFGYWFQT 900
           HPNGKVDETCMQSREIWTGVTYGVAATMILAGMEEEAFKTAEGIFLAGWSEEGFGYWFQT
Sbjct: 841 HPNGKVDETCMQSREIWTGVTYGVAATMILAGMEEEAFKTAEGIFLAGWSEEGFGYWFQT 900

Query: 901 PEAWSTDGHYRSLIYMRPLSIWGMQWALSLPKAILEAPKINVMDRIHVSSASTKFFHHET 960
           PEAWSTDGHYRSLIYMRPLSIWGMQWALSLPKAILEAPKINVMDRIHVSSASTKFFHHET
Sbjct: 901 PEAWSTDGHYRSLIYMRPLSIWGMQWALSLPKAILEAPKINVMDRIHVSSASTKFFHHET 960

Query: 961 GVRRIATKAKCFGDSVFNCAC 982
           GVRRIATKAKCFGDSVFNCAC
Sbjct: 961 GVRRIATKAKCFGDSVFNCAC 981

BLAST of CmaCh08G010320 vs. NCBI nr
Match: KAG6593789.1 (Non-lysosomal glucosylceramidase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 2018.8 bits (5229), Expect = 0.0e+00
Identity = 976/981 (99.49%), Postives = 980/981 (99.90%), Query Frame = 0

Query: 1   MVSGNLFHCRKNSWPPDEYISKSTLQLFDFDSASPPEQAWRRKLNGHANLLKEFSVTFVE 60
           MVSGNLFHCRKNSWPPDEYISKSTLQLFDFDSASPPEQAWRRKLNGHANLLKEFSVTFVE
Sbjct: 1   MVSGNLFHCRKNSWPPDEYISKSTLQLFDFDSASPPEQAWRRKLNGHANLLKEFSVTFVE 60

Query: 61  AIKMVRLGIRLWSYVREEASQGRKAPIDPFTKEACKPSASQGVPLGGMGSGSISRGFRGE 120
           AIKMVRLGIRLWSYVREEASQGRKAPIDPFTKEACKPSASQGVPLGGMGSGSISRGFRGE
Sbjct: 61  AIKMVRLGIRLWSYVREEASQGRKAPIDPFTKEACKPSASQGVPLGGMGSGSISRGFRGE 120

Query: 121 FRQWQIIPGTCEASPVMANQFSIFISRDGGNKKYASVLAPGQHEGIGKAGDSGISSWGWN 180
           FRQWQIIPGTCEASPVMANQFSIFISRDGGNKKYASVLAPGQHEGIGKAGDSGISSWGWN
Sbjct: 121 FRQWQIIPGTCEASPVMANQFSIFISRDGGNKKYASVLAPGQHEGIGKAGDSGISSWGWN 180

Query: 181 LDGQHSTYHALFPRAWTVYDGEPDPELKVSCRQISPFIPHNYRDSSLPTAVFVYTLVNTG 240
           LDGQHSTYHALFPRAWTVYDGEPDPELKVSCRQISPFIPHNYRDSSLPTAVFVYTLVNTG
Sbjct: 181 LDGQHSTYHALFPRAWTVYDGEPDPELKVSCRQISPFIPHNYRDSSLPTAVFVYTLVNTG 240

Query: 241 KERAKVSLVFSWANSIGGISHLSGDHVNEPFIGEDGVSGVLLHHKTAKGNPPVTFAIAAC 300
           +ERAKVSLVFSWANSIGGISHLSGDHVNEPFIGEDGVSGVLLHHKTAKGNPPVTFAIAAC
Sbjct: 241 RERAKVSLVFSWANSIGGISHLSGDHVNEPFIGEDGVSGVLLHHKTAKGNPPVTFAIAAC 300

Query: 301 ETQNVSVTVLPSFGLSEGSGVTAKDMWDKMVQDGQFDRGNFSSGPSVPSSPGETLCAAVA 360
           ETQNVSVTVLPSFGLSEGSGVTAKDMWDKMV DGQFDRGN+SSGPSVPSSPGETLCAAVA
Sbjct: 301 ETQNVSVTVLPSFGLSEGSGVTAKDMWDKMVLDGQFDRGNYSSGPSVPSSPGETLCAAVA 360

Query: 361 ASAWVEPHGKCTVAFSLSWSSPKVKFLKGFSYHRRYTKFYGTSEKAAQTLAHDALTNYKR 420
           ASAWVEPHGKCTVAFSLSWSSPKVKFLKGFSYHRRYTKFYGTSEKAAQTLAHDALTNYKR
Sbjct: 361 ASAWVEPHGKCTVAFSLSWSSPKVKFLKGFSYHRRYTKFYGTSEKAAQTLAHDALTNYKR 420

Query: 421 WEEEIEKWQRPILMDERLPEWYKFTLFNELYFLVAGGTVWIDSSVVGKKMANDHDELVAR 480
           WEEEIEKWQRPILMDERLPEWYKFTLFNELYFLVAGGTVWIDSSVVGKKMANDHDELVAR
Sbjct: 421 WEEEIEKWQRPILMDERLPEWYKFTLFNELYFLVAGGTVWIDSSVVGKKMANDHDELVAR 480

Query: 481 IKEDDIKATEAKVSGRGEEVSRTTTTTTLNDFSGVEFDDEENSTSSSHVSEDELMVPLKS 540
           IKEDD+KATEAKVSGRGEEVSRTTTTTTLNDFSGVEFDDEENSTSSSHVSEDELMVPLKS
Sbjct: 481 IKEDDMKATEAKVSGRGEEVSRTTTTTTLNDFSGVEFDDEENSTSSSHVSEDELMVPLKS 540

Query: 541 GYTDRSYQTYKVMDPGNTEEDVGRFLYLEGVEYVMWCTYDVHFYASFALLELFPKIELNI 600
           GYTDRSYQTYKVMDPGNTEEDVGRFLYLEGVEYVMWCTYDVHFYASFALLELFPKIELNI
Sbjct: 541 GYTDRSYQTYKVMDPGNTEEDVGRFLYLEGVEYVMWCTYDVHFYASFALLELFPKIELNI 600

Query: 601 QRDFAKAVLSEDGRKVKFLADGKYGIRKVRGAVPHDLGTHDPWNEMNAYNIHDTSRWKDL 660
           QRDFAKAVLSEDGRKVKFLADGKYGIRKVRGAVPHDLGTHDPWNEMNAYNIHDTSRWKDL
Sbjct: 601 QRDFAKAVLSEDGRKVKFLADGKYGIRKVRGAVPHDLGTHDPWNEMNAYNIHDTSRWKDL 660

Query: 661 NTKFVLQVYRDFAATRDMSFGVDVWPSVRAAIEYMEQFDRDGDGVIENDGFPDQTYDTWT 720
           NTKFVLQVYRDFAATRDMSFGVDVWPSVRAAIEYMEQFDRDGDGVIENDGFPDQTYDTWT
Sbjct: 661 NTKFVLQVYRDFAATRDMSFGVDVWPSVRAAIEYMEQFDRDGDGVIENDGFPDQTYDTWT 720

Query: 721 VHGISAYCGCLWVAALQAAAAMAHELGDQEFAETCKSKFLKAKPVLEAKLWNGSYFNYDS 780
           VHGISAYCGCLWVAALQAAAAMAHELGDQEFAETCKSKFLKAKPVLEAKLWNGSYFNYDS
Sbjct: 721 VHGISAYCGCLWVAALQAAAAMAHELGDQEFAETCKSKFLKAKPVLEAKLWNGSYFNYDS 780

Query: 781 GSSSNSKSIQADQLAGQWYTASSGLPPLFDDFKIKSALKKIYDFNVMKIRGGRMGAVNGM 840
           GSSSNSKSIQADQLAGQWYTASSGLPPLFDDFKIKSALKKIYDFNVMKI+GGRMGAVNGM
Sbjct: 781 GSSSNSKSIQADQLAGQWYTASSGLPPLFDDFKIKSALKKIYDFNVMKIKGGRMGAVNGM 840

Query: 841 HPNGKVDETCMQSREIWTGVTYGVAATMILAGMEEEAFKTAEGIFLAGWSEEGFGYWFQT 900
           HPNGKVDETCMQSREIWTGVTYGVAATMILAGMEEEAFKTAEGIFLAGWSEEGFGYWFQT
Sbjct: 841 HPNGKVDETCMQSREIWTGVTYGVAATMILAGMEEEAFKTAEGIFLAGWSEEGFGYWFQT 900

Query: 901 PEAWSTDGHYRSLIYMRPLSIWGMQWALSLPKAILEAPKINVMDRIHVSSASTKFFHHET 960
           PEAWSTDGHYRSLIYMRPLSIWGMQWALSLPKAILEAPKINVMDRIHVSSASTKFFHHET
Sbjct: 901 PEAWSTDGHYRSLIYMRPLSIWGMQWALSLPKAILEAPKINVMDRIHVSSASTKFFHHET 960

Query: 961 GVRRIATKAKCFGDSVFNCAC 982
           GVRRIATKAKCFGDSVFNCAC
Sbjct: 961 GVRRIATKAKCFGDSVFNCAC 981

BLAST of CmaCh08G010320 vs. NCBI nr
Match: XP_022964300.1 (non-lysosomal glucosylceramidase [Cucurbita moschata])

HSP 1 Score: 2016.9 bits (5224), Expect = 0.0e+00
Identity = 975/981 (99.39%), Postives = 980/981 (99.90%), Query Frame = 0

Query: 1   MVSGNLFHCRKNSWPPDEYISKSTLQLFDFDSASPPEQAWRRKLNGHANLLKEFSVTFVE 60
           MVSGNLFHCRKNSWPPDEYISKSTLQLFDFDSASPPEQAWRRKLNGHANLLKEFSVTFVE
Sbjct: 1   MVSGNLFHCRKNSWPPDEYISKSTLQLFDFDSASPPEQAWRRKLNGHANLLKEFSVTFVE 60

Query: 61  AIKMVRLGIRLWSYVREEASQGRKAPIDPFTKEACKPSASQGVPLGGMGSGSISRGFRGE 120
           AIKMVRLGIRLWSYVREEASQGRKAPIDPFTKEACKPSASQGVPLGGMGSGSISRGFRGE
Sbjct: 61  AIKMVRLGIRLWSYVREEASQGRKAPIDPFTKEACKPSASQGVPLGGMGSGSISRGFRGE 120

Query: 121 FRQWQIIPGTCEASPVMANQFSIFISRDGGNKKYASVLAPGQHEGIGKAGDSGISSWGWN 180
           FRQWQIIPGTCEASPVMANQFSIFISRDGGNKKYASVLAPGQHEGIGKAGDSGISSWGWN
Sbjct: 121 FRQWQIIPGTCEASPVMANQFSIFISRDGGNKKYASVLAPGQHEGIGKAGDSGISSWGWN 180

Query: 181 LDGQHSTYHALFPRAWTVYDGEPDPELKVSCRQISPFIPHNYRDSSLPTAVFVYTLVNTG 240
           LDGQHSTYHALFPRAWTVYDGEPDPELKVSCRQISPFIPHNYRDSSLPTAVFVYTLVNTG
Sbjct: 181 LDGQHSTYHALFPRAWTVYDGEPDPELKVSCRQISPFIPHNYRDSSLPTAVFVYTLVNTG 240

Query: 241 KERAKVSLVFSWANSIGGISHLSGDHVNEPFIGEDGVSGVLLHHKTAKGNPPVTFAIAAC 300
           +ERAKVSLVFSWANSIGGISHLSGDHVNEPFIGEDGVSGVLLHHKTAKGNPPVTFAIAAC
Sbjct: 241 RERAKVSLVFSWANSIGGISHLSGDHVNEPFIGEDGVSGVLLHHKTAKGNPPVTFAIAAC 300

Query: 301 ETQNVSVTVLPSFGLSEGSGVTAKDMWDKMVQDGQFDRGNFSSGPSVPSSPGETLCAAVA 360
           ETQNVSVTVLPSFGLSEGSGVTAKDMWDKMVQDGQFDRGN+SSGPSVPSSPGETLCAAVA
Sbjct: 301 ETQNVSVTVLPSFGLSEGSGVTAKDMWDKMVQDGQFDRGNYSSGPSVPSSPGETLCAAVA 360

Query: 361 ASAWVEPHGKCTVAFSLSWSSPKVKFLKGFSYHRRYTKFYGTSEKAAQTLAHDALTNYKR 420
           ASAWVEPHGKCTVAFSLSWSSPKVKFLKGFSYHRRYTKFYGTSEKAAQTLAHDALTNYKR
Sbjct: 361 ASAWVEPHGKCTVAFSLSWSSPKVKFLKGFSYHRRYTKFYGTSEKAAQTLAHDALTNYKR 420

Query: 421 WEEEIEKWQRPILMDERLPEWYKFTLFNELYFLVAGGTVWIDSSVVGKKMANDHDELVAR 480
           WEEEIEKWQ PILMDERLPEWYKFTLFNELYFLVAGGTVWIDSSVVGKKMANDHDELVAR
Sbjct: 421 WEEEIEKWQGPILMDERLPEWYKFTLFNELYFLVAGGTVWIDSSVVGKKMANDHDELVAR 480

Query: 481 IKEDDIKATEAKVSGRGEEVSRTTTTTTLNDFSGVEFDDEENSTSSSHVSEDELMVPLKS 540
           IKE+DIKATEA+VSGRGEEVSRTTTTTTLNDFSGVEFDDEENSTSSSHVSEDELMVPLKS
Sbjct: 481 IKEEDIKATEAEVSGRGEEVSRTTTTTTLNDFSGVEFDDEENSTSSSHVSEDELMVPLKS 540

Query: 541 GYTDRSYQTYKVMDPGNTEEDVGRFLYLEGVEYVMWCTYDVHFYASFALLELFPKIELNI 600
           GYTDRSYQTYKVMDPGNTEEDVGRFLYLEGVEYVMWCTYDVHFYASFALLELFPKIELNI
Sbjct: 541 GYTDRSYQTYKVMDPGNTEEDVGRFLYLEGVEYVMWCTYDVHFYASFALLELFPKIELNI 600

Query: 601 QRDFAKAVLSEDGRKVKFLADGKYGIRKVRGAVPHDLGTHDPWNEMNAYNIHDTSRWKDL 660
           QRDFAKAVLSEDGRKVKFLADGKYGIRKVRGAVPHDLGTHDPWNEMNAYNIHDTSRWKDL
Sbjct: 601 QRDFAKAVLSEDGRKVKFLADGKYGIRKVRGAVPHDLGTHDPWNEMNAYNIHDTSRWKDL 660

Query: 661 NTKFVLQVYRDFAATRDMSFGVDVWPSVRAAIEYMEQFDRDGDGVIENDGFPDQTYDTWT 720
           NTKFVLQVYRDFAATRDMSFGVDVWPSVRAAIEYMEQFDRDGDGVIENDGFPDQTYDTWT
Sbjct: 661 NTKFVLQVYRDFAATRDMSFGVDVWPSVRAAIEYMEQFDRDGDGVIENDGFPDQTYDTWT 720

Query: 721 VHGISAYCGCLWVAALQAAAAMAHELGDQEFAETCKSKFLKAKPVLEAKLWNGSYFNYDS 780
           VHGISAYCGCLWVAALQAAAAMAHELGDQEFAETCKSKFLKAKPVLEAKLWNGSYFNYDS
Sbjct: 721 VHGISAYCGCLWVAALQAAAAMAHELGDQEFAETCKSKFLKAKPVLEAKLWNGSYFNYDS 780

Query: 781 GSSSNSKSIQADQLAGQWYTASSGLPPLFDDFKIKSALKKIYDFNVMKIRGGRMGAVNGM 840
           GSSSNSKSIQADQLAGQWYTASSGLPPLFDDFKIKSALKKIYDFNVMKI+GGRMGAVNGM
Sbjct: 781 GSSSNSKSIQADQLAGQWYTASSGLPPLFDDFKIKSALKKIYDFNVMKIKGGRMGAVNGM 840

Query: 841 HPNGKVDETCMQSREIWTGVTYGVAATMILAGMEEEAFKTAEGIFLAGWSEEGFGYWFQT 900
           HPNGKVDETCMQSREIWTGVTYGVAATMILAGMEEEAFKTAEGIFLAGWSEEGFGYWFQT
Sbjct: 841 HPNGKVDETCMQSREIWTGVTYGVAATMILAGMEEEAFKTAEGIFLAGWSEEGFGYWFQT 900

Query: 901 PEAWSTDGHYRSLIYMRPLSIWGMQWALSLPKAILEAPKINVMDRIHVSSASTKFFHHET 960
           PEAWSTDGHYRSLIYMRPLSIWGMQWALSLPKAILEAPKINVMDRIHVSSASTKFFHHET
Sbjct: 901 PEAWSTDGHYRSLIYMRPLSIWGMQWALSLPKAILEAPKINVMDRIHVSSASTKFFHHET 960

Query: 961 GVRRIATKAKCFGDSVFNCAC 982
           GVRRIATKAKCFGDSVFNCAC
Sbjct: 961 GVRRIATKAKCFGDSVFNCAC 981

BLAST of CmaCh08G010320 vs. NCBI nr
Match: XP_023514140.1 (non-lysosomal glucosylceramidase [Cucurbita pepo subsp. pepo] >XP_023514141.1 non-lysosomal glucosylceramidase [Cucurbita pepo subsp. pepo])

HSP 1 Score: 2015.7 bits (5221), Expect = 0.0e+00
Identity = 974/981 (99.29%), Postives = 980/981 (99.90%), Query Frame = 0

Query: 1   MVSGNLFHCRKNSWPPDEYISKSTLQLFDFDSASPPEQAWRRKLNGHANLLKEFSVTFVE 60
           MVSGNLFHCRKNSWPPDEYISKSTLQLFDFDSASPPEQAWRRKLNGHANLLKEFSVTFVE
Sbjct: 1   MVSGNLFHCRKNSWPPDEYISKSTLQLFDFDSASPPEQAWRRKLNGHANLLKEFSVTFVE 60

Query: 61  AIKMVRLGIRLWSYVREEASQGRKAPIDPFTKEACKPSASQGVPLGGMGSGSISRGFRGE 120
           AIKMVRLGIRLWSYVREEASQGRKAPIDPFTKEACKPSASQGVPLGGMGSGSISRGFRGE
Sbjct: 61  AIKMVRLGIRLWSYVREEASQGRKAPIDPFTKEACKPSASQGVPLGGMGSGSISRGFRGE 120

Query: 121 FRQWQIIPGTCEASPVMANQFSIFISRDGGNKKYASVLAPGQHEGIGKAGDSGISSWGWN 180
           FRQWQIIPGTCEASPVMANQFSIFISRDGGNKKYASVLAPGQHEGIGKAGDSGISSWGWN
Sbjct: 121 FRQWQIIPGTCEASPVMANQFSIFISRDGGNKKYASVLAPGQHEGIGKAGDSGISSWGWN 180

Query: 181 LDGQHSTYHALFPRAWTVYDGEPDPELKVSCRQISPFIPHNYRDSSLPTAVFVYTLVNTG 240
           LDGQHSTYHALFPRAWTVYDGEPDPELKVSCRQISPFIPHNYRDSSLPTAVFVYTLVNTG
Sbjct: 181 LDGQHSTYHALFPRAWTVYDGEPDPELKVSCRQISPFIPHNYRDSSLPTAVFVYTLVNTG 240

Query: 241 KERAKVSLVFSWANSIGGISHLSGDHVNEPFIGEDGVSGVLLHHKTAKGNPPVTFAIAAC 300
           +ERAKVSLVFSWANSIGGISHLSGDHVNEPFIGEDGVSGVLLHHKTAKGNPPVTFAIAAC
Sbjct: 241 RERAKVSLVFSWANSIGGISHLSGDHVNEPFIGEDGVSGVLLHHKTAKGNPPVTFAIAAC 300

Query: 301 ETQNVSVTVLPSFGLSEGSGVTAKDMWDKMVQDGQFDRGNFSSGPSVPSSPGETLCAAVA 360
           ETQNVSVTVLPSFGLSEGSGVTAKDMWDKMVQDGQFDRGNFSSGPSVPSSPGETLCAAVA
Sbjct: 301 ETQNVSVTVLPSFGLSEGSGVTAKDMWDKMVQDGQFDRGNFSSGPSVPSSPGETLCAAVA 360

Query: 361 ASAWVEPHGKCTVAFSLSWSSPKVKFLKGFSYHRRYTKFYGTSEKAAQTLAHDALTNYKR 420
           ASAWVEPHGKCTVAFSLSWSSPKVKFLKGFSY RRYTKFYGTSEKAAQTLAHDALTNYKR
Sbjct: 361 ASAWVEPHGKCTVAFSLSWSSPKVKFLKGFSYRRRYTKFYGTSEKAAQTLAHDALTNYKR 420

Query: 421 WEEEIEKWQRPILMDERLPEWYKFTLFNELYFLVAGGTVWIDSSVVGKKMANDHDELVAR 480
           WEEEIEKWQRPILMDERLPEWYKFTLFNELYFLVAGGTVWIDSSVVGKKMANDHDELVAR
Sbjct: 421 WEEEIEKWQRPILMDERLPEWYKFTLFNELYFLVAGGTVWIDSSVVGKKMANDHDELVAR 480

Query: 481 IKEDDIKATEAKVSGRGEEVSRTTTTTTLNDFSGVEFDDEENSTSSSHVSEDELMVPLKS 540
           IKEDD+KATEAKVSGRGEEVSRTTTTTTLNDFSGVEFD+EENSTSSSHVSEDELMVPLKS
Sbjct: 481 IKEDDMKATEAKVSGRGEEVSRTTTTTTLNDFSGVEFDEEENSTSSSHVSEDELMVPLKS 540

Query: 541 GYTDRSYQTYKVMDPGNTEEDVGRFLYLEGVEYVMWCTYDVHFYASFALLELFPKIELNI 600
           GYTDRSYQTYKVMDPGNTEEDVGRFLYLEGVEYVMWCTYDVHFYASFALLELFPKIELNI
Sbjct: 541 GYTDRSYQTYKVMDPGNTEEDVGRFLYLEGVEYVMWCTYDVHFYASFALLELFPKIELNI 600

Query: 601 QRDFAKAVLSEDGRKVKFLADGKYGIRKVRGAVPHDLGTHDPWNEMNAYNIHDTSRWKDL 660
           QRDFAKAVLSEDGRKVKFLADGKYGIRKVRGAVPHDLGTHDPWNEMNAYNIHDTSRWKDL
Sbjct: 601 QRDFAKAVLSEDGRKVKFLADGKYGIRKVRGAVPHDLGTHDPWNEMNAYNIHDTSRWKDL 660

Query: 661 NTKFVLQVYRDFAATRDMSFGVDVWPSVRAAIEYMEQFDRDGDGVIENDGFPDQTYDTWT 720
           NTKFVLQVYRDFAATRDM+FGVDVWPSVRAAIEYMEQFDRDGDGVIENDGFPDQTYDTWT
Sbjct: 661 NTKFVLQVYRDFAATRDMAFGVDVWPSVRAAIEYMEQFDRDGDGVIENDGFPDQTYDTWT 720

Query: 721 VHGISAYCGCLWVAALQAAAAMAHELGDQEFAETCKSKFLKAKPVLEAKLWNGSYFNYDS 780
           VHGISAYCGCLWVAALQAAAAMAHELGDQEFAETCKSKFLKAKPVLEAKLWNGSYFNYDS
Sbjct: 721 VHGISAYCGCLWVAALQAAAAMAHELGDQEFAETCKSKFLKAKPVLEAKLWNGSYFNYDS 780

Query: 781 GSSSNSKSIQADQLAGQWYTASSGLPPLFDDFKIKSALKKIYDFNVMKIRGGRMGAVNGM 840
           GSSSNSKSIQADQLAGQWYTASSGLPPLFDDFKIKSALKKIYDFNVMKI+GGRMGAVNGM
Sbjct: 781 GSSSNSKSIQADQLAGQWYTASSGLPPLFDDFKIKSALKKIYDFNVMKIKGGRMGAVNGM 840

Query: 841 HPNGKVDETCMQSREIWTGVTYGVAATMILAGMEEEAFKTAEGIFLAGWSEEGFGYWFQT 900
           HPNGKVDETCMQSREIWTGVTYGVAATMILAGMEEEAFKTAEGIFLAGWSE+GFGYWFQT
Sbjct: 841 HPNGKVDETCMQSREIWTGVTYGVAATMILAGMEEEAFKTAEGIFLAGWSEDGFGYWFQT 900

Query: 901 PEAWSTDGHYRSLIYMRPLSIWGMQWALSLPKAILEAPKINVMDRIHVSSASTKFFHHET 960
           PEAWSTDGHYRSLIYMRPLSIWGMQWALSLPKAILEAPKINVMDRIHVSSASTKFFHHET
Sbjct: 901 PEAWSTDGHYRSLIYMRPLSIWGMQWALSLPKAILEAPKINVMDRIHVSSASTKFFHHET 960

Query: 961 GVRRIATKAKCFGDSVFNCAC 982
           GVRRIATKAKCFGDSVFNCAC
Sbjct: 961 GVRRIATKAKCFGDSVFNCAC 981

BLAST of CmaCh08G010320 vs. NCBI nr
Match: KAG7026118.1 (Non-lysosomal glucosylceramidase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 2006.1 bits (5196), Expect = 0.0e+00
Identity = 977/1010 (96.73%), Postives = 981/1010 (97.13%), Query Frame = 0

Query: 1    MVSGNLFHCRKNSWPPDEYISKSTLQLFDFDSASPPEQAWRRKLNGHANLLKEFSVTFVE 60
            MVSGNLFHCRKNSWPPDEYISKSTLQLFDFDSASPPEQAWRRKLNGHANLLKEFSVTFVE
Sbjct: 1    MVSGNLFHCRKNSWPPDEYISKSTLQLFDFDSASPPEQAWRRKLNGHANLLKEFSVTFVE 60

Query: 61   AIKMVRLGIRLWSYVREEASQGRKAPIDPFTKEACKPSASQGVPLGGMGSGSISRGFRGE 120
            AIKMVRLGIRLWSYVREEASQGRKAPIDPFTKEACKPSASQGVPLGGMGSGSISRGFRGE
Sbjct: 61   AIKMVRLGIRLWSYVREEASQGRKAPIDPFTKEACKPSASQGVPLGGMGSGSISRGFRGE 120

Query: 121  FRQWQIIPGTCEASPVMANQFSIFISRDGGNKKYASVLAPGQHEGIGKAGDSGISSWGWN 180
            FRQWQIIPGTCEASPVMANQFSIFISRDGGNKKYASVLAPGQHEGIGKAGDSGISSWGWN
Sbjct: 121  FRQWQIIPGTCEASPVMANQFSIFISRDGGNKKYASVLAPGQHEGIGKAGDSGISSWGWN 180

Query: 181  LDGQHSTYHALFPRAWTVYDGEPDPELKVSCRQISPFIPHNYRDSSLPTAVFVYTLVNTG 240
            LDGQHSTYHALFPRAWTVYDGEPDPELKVSCRQISPFIPHNYRDSSLPTAVFVYTLVNTG
Sbjct: 181  LDGQHSTYHALFPRAWTVYDGEPDPELKVSCRQISPFIPHNYRDSSLPTAVFVYTLVNTG 240

Query: 241  KERAKVSLVFSWANSIGGISHLSGDHVNEPFIGEDGVSGVLLHHK--------------- 300
            +ERAKVSLVFSWANSIGGISHLSGDHVNEPFIGEDGVSGVLLHHK               
Sbjct: 241  RERAKVSLVFSWANSIGGISHLSGDHVNEPFIGEDGVSGVLLHHKQVTKPYTLFYACGYG 300

Query: 301  --------------TAKGNPPVTFAIAACETQNVSVTVLPSFGLSEGSGVTAKDMWDKMV 360
                          TAKGNPPVTFAIAACETQNVSVTVLPSFGLSEGSGVTAKDMWDKMV
Sbjct: 301  RPVKNLKEMCICFRTAKGNPPVTFAIAACETQNVSVTVLPSFGLSEGSGVTAKDMWDKMV 360

Query: 361  QDGQFDRGNFSSGPSVPSSPGETLCAAVAASAWVEPHGKCTVAFSLSWSSPKVKFLKGFS 420
            QDGQFDRGN+SSGPSVPSSPGETLCAAVAASAWVEPHGKCTVAFSLSWSSPKVKFLKGFS
Sbjct: 361  QDGQFDRGNYSSGPSVPSSPGETLCAAVAASAWVEPHGKCTVAFSLSWSSPKVKFLKGFS 420

Query: 421  YHRRYTKFYGTSEKAAQTLAHDALTNYKRWEEEIEKWQRPILMDERLPEWYKFTLFNELY 480
            YHRRYTKFYGTSEKAAQTLAHDALTNYKRWEEEIEKWQRPILMDERLPEWYKFTLFNELY
Sbjct: 421  YHRRYTKFYGTSEKAAQTLAHDALTNYKRWEEEIEKWQRPILMDERLPEWYKFTLFNELY 480

Query: 481  FLVAGGTVWIDSSVVGKKMANDHDELVARIKEDDIKATEAKVSGRGEEVSRTTTTTTLND 540
            FLVAGGTVWIDSSVVGKKMANDHDELVARIKEDD+KATEAKVSGRGEEVSRTTTTTTLND
Sbjct: 481  FLVAGGTVWIDSSVVGKKMANDHDELVARIKEDDMKATEAKVSGRGEEVSRTTTTTTLND 540

Query: 541  FSGVEFDDEENSTSSSHVSEDELMVPLKSGYTDRSYQTYKVMDPGNTEEDVGRFLYLEGV 600
            FSGVEFDDEENSTSSSHVSEDELMVPLKSGYTDRSYQTYKVMDPGNTEEDVGRFLYLEGV
Sbjct: 541  FSGVEFDDEENSTSSSHVSEDELMVPLKSGYTDRSYQTYKVMDPGNTEEDVGRFLYLEGV 600

Query: 601  EYVMWCTYDVHFYASFALLELFPKIELNIQRDFAKAVLSEDGRKVKFLADGKYGIRKVRG 660
            EYVMWCTYDVHFYASFALLELFPKIELNIQRDFAKAVLSEDGRKVKFLADGKYGIRKVRG
Sbjct: 601  EYVMWCTYDVHFYASFALLELFPKIELNIQRDFAKAVLSEDGRKVKFLADGKYGIRKVRG 660

Query: 661  AVPHDLGTHDPWNEMNAYNIHDTSRWKDLNTKFVLQVYRDFAATRDMSFGVDVWPSVRAA 720
            AVPHDLGTHDPWNEMNAYNIHDTSRWKDLNTKFVLQVYRDFAATRDMSFGVDVWPSVRAA
Sbjct: 661  AVPHDLGTHDPWNEMNAYNIHDTSRWKDLNTKFVLQVYRDFAATRDMSFGVDVWPSVRAA 720

Query: 721  IEYMEQFDRDGDGVIENDGFPDQTYDTWTVHGISAYCGCLWVAALQAAAAMAHELGDQEF 780
            IEYMEQFDRDGDGVIENDGFPDQTYDTWTVHGISAYCGCLWVAALQAAAAMAHELGDQEF
Sbjct: 721  IEYMEQFDRDGDGVIENDGFPDQTYDTWTVHGISAYCGCLWVAALQAAAAMAHELGDQEF 780

Query: 781  AETCKSKFLKAKPVLEAKLWNGSYFNYDSGSSSNSKSIQADQLAGQWYTASSGLPPLFDD 840
            AETCKSKFLKAKPVLEAKLWNGSYFNYDSGSSSNSKSIQADQLAGQWYTASSGLPPLFDD
Sbjct: 781  AETCKSKFLKAKPVLEAKLWNGSYFNYDSGSSSNSKSIQADQLAGQWYTASSGLPPLFDD 840

Query: 841  FKIKSALKKIYDFNVMKIRGGRMGAVNGMHPNGKVDETCMQSREIWTGVTYGVAATMILA 900
            FKIKSALKKIYDFNVMKI+GGRMGAVNGMHPNGKVDETCMQSREIWTGVTYGVAATMILA
Sbjct: 841  FKIKSALKKIYDFNVMKIKGGRMGAVNGMHPNGKVDETCMQSREIWTGVTYGVAATMILA 900

Query: 901  GMEEEAFKTAEGIFLAGWSEEGFGYWFQTPEAWSTDGHYRSLIYMRPLSIWGMQWALSLP 960
            GMEEEAFKTAEGIFLAGWSEEGFGYWFQTPEAWSTDGHYRSLIYMRPLSIWGMQWALSLP
Sbjct: 901  GMEEEAFKTAEGIFLAGWSEEGFGYWFQTPEAWSTDGHYRSLIYMRPLSIWGMQWALSLP 960

Query: 961  KAILEAPKINVMDRIHVSSASTKFFHHETGVRRIATKAKCFGDSVFNCAC 982
            KAILEAPKINVMDRIHVSSASTKFFHHETGVRRIATKAKCFGDSVFNCAC
Sbjct: 961  KAILEAPKINVMDRIHVSSASTKFFHHETGVRRIATKAKCFGDSVFNCAC 1010

BLAST of CmaCh08G010320 vs. TAIR 10
Match: AT3G24180.1 (Beta-glucosidase, GBA2 type family protein )

HSP 1 Score: 1527.7 bits (3954), Expect = 0.0e+00
Identity = 739/983 (75.18%), Postives = 833/983 (84.74%), Query Frame = 0

Query: 1   MVSGNLFHCRKNSWPPDEYISKSTLQLFDFDSASPPEQAWRRKLNGHANLLKEFSVTFVE 60
           MV   LFH RK+SWP +E+IS+STLQL DFDSA+PP  AWRR+LN HAN+LKEF++TF E
Sbjct: 1   MVGATLFHRRKHSWPTEEFISRSTLQLLDFDSAAPPPHAWRRRLNCHANILKEFTITFRE 60

Query: 61  AIKMVRLGIRLWSYVREEASQGRKAPIDPFTKEACKPSASQGVPLGGMGSGSISRGFRGE 120
           AIKMVRLGIRLWSYVREEAS GRKAPIDPFTKE CKPSASQGVPLGGMGSGSISRGFRGE
Sbjct: 61  AIKMVRLGIRLWSYVREEASHGRKAPIDPFTKENCKPSASQGVPLGGMGSGSISRGFRGE 120

Query: 121 FRQWQIIPGTCEASPVMANQFSIFISRDGGNKKYASVLAPGQHEGIGKAGDSGISSWGWN 180
           F+QWQI PGTC+ SP+M+NQFSIFISRDGG+KKYASVL+PGQH  +GK+ D G+SSWGWN
Sbjct: 121 FKQWQITPGTCDPSPMMSNQFSIFISRDGGHKKYASVLSPGQHGSLGKSRDKGLSSWGWN 180

Query: 181 LDGQHSTYHALFPRAWTVYDGEPDPELKVSCRQISPFIPHNYRDSSLPTAVFVYTLVNTG 240
           L+GQHSTYHALFPRAWT+YDGEPDPELK+SCRQISPFIP+NYRDSSLP AVFVYTLVNTG
Sbjct: 181 LNGQHSTYHALFPRAWTIYDGEPDPELKISCRQISPFIPNNYRDSSLPAAVFVYTLVNTG 240

Query: 241 KERAKVSLVFSWANSIGGISHLSGDHVNEPFIGEDGVSGVLLHHKTAKGNPPVTFAIAAC 300
           KERAKVSL+F+WANS+GG SH+SG HVNEPFIGEDGVSGVLLHHKT KGNPPVTFAIAA 
Sbjct: 241 KERAKVSLLFTWANSMGGTSHMSGGHVNEPFIGEDGVSGVLLHHKTGKGNPPVTFAIAAS 300

Query: 301 ETQNVSVTVLPSFGLSEGSGVTAKDMWDKMVQDGQFDRGNFSSGPSVPSSPGETLCAAVA 360
           ETQNV+VTVLP FGLSE S  TAKDMWD M QDG+FD+ NF+SGPS PS  G+T+CAAV+
Sbjct: 301 ETQNVNVTVLPCFGLSEDSSFTAKDMWDTMEQDGKFDQENFNSGPSTPSLAGDTICAAVS 360

Query: 361 ASAWVEPHGKCTVAFSLSWSSPKVKFLKGFSYHRRYTKFYGTSEKAAQTLAHDALTNYKR 420
           ASAWVE HGKCTV+F+LSWSSPKVKF KG +Y RRYTKFYGTS +AA  L HDALTNYKR
Sbjct: 361 ASAWVEAHGKCTVSFALSWSSPKVKFSKGSTYDRRYTKFYGTSPRAALDLVHDALTNYKR 420

Query: 421 WEEEIEKWQRPILMDERLPEWYKFTLFNELYFLVAGGTVWIDSSVVGKKMANDHDELVAR 480
           WEE+IE WQ PIL DERLPEWYKFTLFNELYFLVAGGTVWIDSS +     + H      
Sbjct: 421 WEEDIEAWQNPILRDERLPEWYKFTLFNELYFLVAGGTVWIDSSSLNANGNSQH------ 480

Query: 481 IKEDDIKATEAKVSGRGEEVSRTTTTTTLNDFSGVEFDDEENSTSSSHVSEDEL-MVPLK 540
            ++  +  ++ KV G             +ND    + +D  N  S    S DE+  +  +
Sbjct: 481 -QQSGLGNSDGKVGG-----------LDIND----QRNDLGNGNSVGVKSNDEVSAIHNR 540

Query: 541 SG-YTDRSYQTYKVMDPGNTEEDVGRFLYLEGVEYVMWCTYDVHFYASFALLELFPKIEL 600
           +G + D  +     +D G   +DVGRFLYLEGVEYVMWCTYDVHFYAS+ALL LFPKIEL
Sbjct: 541 NGLFVDTPH-----VDDG---DDVGRFLYLEGVEYVMWCTYDVHFYASYALLMLFPKIEL 600

Query: 601 NIQRDFAKAVLSEDGRKVKFLADGKYGIRKVRGAVPHDLGTHDPWNEMNAYNIHDTSRWK 660
           NIQRDFAKAVLSEDGRKVKFLA+G  GIRKVRGAVPHDLG HDPWNEMNAYNIHDTSRWK
Sbjct: 601 NIQRDFAKAVLSEDGRKVKFLAEGNVGIRKVRGAVPHDLGMHDPWNEMNAYNIHDTSRWK 660

Query: 661 DLNTKFVLQVYRDFAATRDMSFGVDVWPSVRAAIEYMEQFDRDGDGVIENDGFPDQTYDT 720
           DLN KFVLQVYRDFAAT D  FG+DVWP+VRAA+EYMEQFDRD D +IENDGFPDQTYDT
Sbjct: 661 DLNPKFVLQVYRDFAATGDYQFGIDVWPAVRAAMEYMEQFDRDNDDLIENDGFPDQTYDT 720

Query: 721 WTVHGISAYCGCLWVAALQAAAAMAHELGDQEFAETCKSKFLKAKPVLEAKLWNGSYFNY 780
           WTVHG+SAYCGCLW+AALQAAAAMA ++GD+ FAE CK+KFL AK  LE KLWNGSYFNY
Sbjct: 721 WTVHGVSAYCGCLWLAALQAAAAMALQIGDKFFAELCKNKFLNAKAALETKLWNGSYFNY 780

Query: 781 DSGSSSNSKSIQADQLAGQWYTASSGLPPLFDDFKIKSALKKIYDFNVMKIRGGRMGAVN 840
           DSGSSSNSKSIQ DQLAGQWY ASSGLPPLF++ KI+S ++KI+DFNVMK +GG+MGAVN
Sbjct: 781 DSGSSSNSKSIQTDQLAGQWYAASSGLPPLFEESKIRSTMQKIFDFNVMKTKGGKMGAVN 840

Query: 841 GMHPNGKVDETCMQSREIWTGVTYGVAATMILAGMEEEAFKTAEGIFLAGWSEEGFGYWF 900
           GMHP+GKVD+TCMQSREIWTGVTY  AATMIL+GMEE+ F TAEGIF AGWSEEGFGYWF
Sbjct: 841 GMHPDGKVDDTCMQSREIWTGVTYAAAATMILSGMEEQGFTTAEGIFTAGWSEEGFGYWF 900

Query: 901 QTPEAWSTDGHYRSLIYMRPLSIWGMQWALSLPKAILEAPKINVMDRIHVSSASTKFFHH 960
           QTPE W+ DGHYRSLIYMRPL+IWGMQWALSLPKAIL+AP+IN+MDR+H+S  S +F ++
Sbjct: 901 QTPEGWTMDGHYRSLIYMRPLAIWGMQWALSLPKAILDAPQINMMDRVHLSPRSRRFSNN 950

Query: 961 ETGVRRIATKAKCFGDSVFNCAC 982
              V+    KAKCFG+S  +C+C
Sbjct: 961 FKVVKH---KAKCFGNSALSCSC 950

BLAST of CmaCh08G010320 vs. TAIR 10
Match: AT3G24180.2 (Beta-glucosidase, GBA2 type family protein )

HSP 1 Score: 1527.7 bits (3954), Expect = 0.0e+00
Identity = 739/983 (75.18%), Postives = 833/983 (84.74%), Query Frame = 0

Query: 1   MVSGNLFHCRKNSWPPDEYISKSTLQLFDFDSASPPEQAWRRKLNGHANLLKEFSVTFVE 60
           MV   LFH RK+SWP +E+IS+STLQL DFDSA+PP  AWRR+LN HAN+LKEF++TF E
Sbjct: 1   MVGATLFHRRKHSWPTEEFISRSTLQLLDFDSAAPPPHAWRRRLNCHANILKEFTITFRE 60

Query: 61  AIKMVRLGIRLWSYVREEASQGRKAPIDPFTKEACKPSASQGVPLGGMGSGSISRGFRGE 120
           AIKMVRLGIRLWSYVREEAS GRKAPIDPFTKE CKPSASQGVPLGGMGSGSISRGFRGE
Sbjct: 61  AIKMVRLGIRLWSYVREEASHGRKAPIDPFTKENCKPSASQGVPLGGMGSGSISRGFRGE 120

Query: 121 FRQWQIIPGTCEASPVMANQFSIFISRDGGNKKYASVLAPGQHEGIGKAGDSGISSWGWN 180
           F+QWQI PGTC+ SP+M+NQFSIFISRDGG+KKYASVL+PGQH  +GK+ D G+SSWGWN
Sbjct: 121 FKQWQITPGTCDPSPMMSNQFSIFISRDGGHKKYASVLSPGQHGSLGKSRDKGLSSWGWN 180

Query: 181 LDGQHSTYHALFPRAWTVYDGEPDPELKVSCRQISPFIPHNYRDSSLPTAVFVYTLVNTG 240
           L+GQHSTYHALFPRAWT+YDGEPDPELK+SCRQISPFIP+NYRDSSLP AVFVYTLVNTG
Sbjct: 181 LNGQHSTYHALFPRAWTIYDGEPDPELKISCRQISPFIPNNYRDSSLPAAVFVYTLVNTG 240

Query: 241 KERAKVSLVFSWANSIGGISHLSGDHVNEPFIGEDGVSGVLLHHKTAKGNPPVTFAIAAC 300
           KERAKVSL+F+WANS+GG SH+SG HVNEPFIGEDGVSGVLLHHKT KGNPPVTFAIAA 
Sbjct: 241 KERAKVSLLFTWANSMGGTSHMSGGHVNEPFIGEDGVSGVLLHHKTGKGNPPVTFAIAAS 300

Query: 301 ETQNVSVTVLPSFGLSEGSGVTAKDMWDKMVQDGQFDRGNFSSGPSVPSSPGETLCAAVA 360
           ETQNV+VTVLP FGLSE S  TAKDMWD M QDG+FD+ NF+SGPS PS  G+T+CAAV+
Sbjct: 301 ETQNVNVTVLPCFGLSEDSSFTAKDMWDTMEQDGKFDQENFNSGPSTPSLAGDTICAAVS 360

Query: 361 ASAWVEPHGKCTVAFSLSWSSPKVKFLKGFSYHRRYTKFYGTSEKAAQTLAHDALTNYKR 420
           ASAWVE HGKCTV+F+LSWSSPKVKF KG +Y RRYTKFYGTS +AA  L HDALTNYKR
Sbjct: 361 ASAWVEAHGKCTVSFALSWSSPKVKFSKGSTYDRRYTKFYGTSPRAALDLVHDALTNYKR 420

Query: 421 WEEEIEKWQRPILMDERLPEWYKFTLFNELYFLVAGGTVWIDSSVVGKKMANDHDELVAR 480
           WEE+IE WQ PIL DERLPEWYKFTLFNELYFLVAGGTVWIDSS +     + H      
Sbjct: 421 WEEDIEAWQNPILRDERLPEWYKFTLFNELYFLVAGGTVWIDSSSLNANGNSQH------ 480

Query: 481 IKEDDIKATEAKVSGRGEEVSRTTTTTTLNDFSGVEFDDEENSTSSSHVSEDEL-MVPLK 540
            ++  +  ++ KV G             +ND    + +D  N  S    S DE+  +  +
Sbjct: 481 -QQSGLGNSDGKVGG-----------LDIND----QRNDLGNGNSVGVKSNDEVSAIHNR 540

Query: 541 SG-YTDRSYQTYKVMDPGNTEEDVGRFLYLEGVEYVMWCTYDVHFYASFALLELFPKIEL 600
           +G + D  +     +D G   +DVGRFLYLEGVEYVMWCTYDVHFYAS+ALL LFPKIEL
Sbjct: 541 NGLFVDTPH-----VDDG---DDVGRFLYLEGVEYVMWCTYDVHFYASYALLMLFPKIEL 600

Query: 601 NIQRDFAKAVLSEDGRKVKFLADGKYGIRKVRGAVPHDLGTHDPWNEMNAYNIHDTSRWK 660
           NIQRDFAKAVLSEDGRKVKFLA+G  GIRKVRGAVPHDLG HDPWNEMNAYNIHDTSRWK
Sbjct: 601 NIQRDFAKAVLSEDGRKVKFLAEGNVGIRKVRGAVPHDLGMHDPWNEMNAYNIHDTSRWK 660

Query: 661 DLNTKFVLQVYRDFAATRDMSFGVDVWPSVRAAIEYMEQFDRDGDGVIENDGFPDQTYDT 720
           DLN KFVLQVYRDFAAT D  FG+DVWP+VRAA+EYMEQFDRD D +IENDGFPDQTYDT
Sbjct: 661 DLNPKFVLQVYRDFAATGDYQFGIDVWPAVRAAMEYMEQFDRDNDDLIENDGFPDQTYDT 720

Query: 721 WTVHGISAYCGCLWVAALQAAAAMAHELGDQEFAETCKSKFLKAKPVLEAKLWNGSYFNY 780
           WTVHG+SAYCGCLW+AALQAAAAMA ++GD+ FAE CK+KFL AK  LE KLWNGSYFNY
Sbjct: 721 WTVHGVSAYCGCLWLAALQAAAAMALQIGDKFFAELCKNKFLNAKAALETKLWNGSYFNY 780

Query: 781 DSGSSSNSKSIQADQLAGQWYTASSGLPPLFDDFKIKSALKKIYDFNVMKIRGGRMGAVN 840
           DSGSSSNSKSIQ DQLAGQWY ASSGLPPLF++ KI+S ++KI+DFNVMK +GG+MGAVN
Sbjct: 781 DSGSSSNSKSIQTDQLAGQWYAASSGLPPLFEESKIRSTMQKIFDFNVMKTKGGKMGAVN 840

Query: 841 GMHPNGKVDETCMQSREIWTGVTYGVAATMILAGMEEEAFKTAEGIFLAGWSEEGFGYWF 900
           GMHP+GKVD+TCMQSREIWTGVTY  AATMIL+GMEE+ F TAEGIF AGWSEEGFGYWF
Sbjct: 841 GMHPDGKVDDTCMQSREIWTGVTYAAAATMILSGMEEQGFTTAEGIFTAGWSEEGFGYWF 900

Query: 901 QTPEAWSTDGHYRSLIYMRPLSIWGMQWALSLPKAILEAPKINVMDRIHVSSASTKFFHH 960
           QTPE W+ DGHYRSLIYMRPL+IWGMQWALSLPKAIL+AP+IN+MDR+H+S  S +F ++
Sbjct: 901 QTPEGWTMDGHYRSLIYMRPLAIWGMQWALSLPKAILDAPQINMMDRVHLSPRSRRFSNN 950

Query: 961 ETGVRRIATKAKCFGDSVFNCAC 982
              V+    KAKCFG+S  +C+C
Sbjct: 961 FKVVKH---KAKCFGNSALSCSC 950

BLAST of CmaCh08G010320 vs. TAIR 10
Match: AT5G49900.1 (Beta-glucosidase, GBA2 type family protein )

HSP 1 Score: 951.4 bits (2458), Expect = 5.5e-277
Identity = 484/900 (53.78%), Postives = 616/900 (68.44%), Query Frame = 0

Query: 31  DSASPPEQAWRRKLNGHANLLKEFSVTFVEAIKMVRLGIRLWSYVREEASQGRKAPIDPF 90
           D A P    W+RK++      +EF+++  E  ++  +GIRLW   REEA++GR A IDPF
Sbjct: 24  DPAVPASLTWQRKIDSDVKAPREFNLSVKEIFQLAPVGIRLWFLCREEAAKGRLAFIDPF 83

Query: 91  TKEACKPSASQGVPLGGMGSGSISRGFRGEFRQWQIIPGTCEASPVMANQFSIFISRDGG 150
           +K +   ++S GVPLGG+G+GSI R F+GEF++WQ+ P  CE  PV+ANQFS F+SR  G
Sbjct: 84  SKHSV--TSSHGVPLGGIGAGSIGRSFKGEFQRWQLFPPKCEDEPVLANQFSAFVSRANG 143

Query: 151 NKKYASVLAPGQHEGIGKAGDSGISSWGWNLDGQHSTYHALFPRAWTVYDGEPDPELKVS 210
            KKY+SVL P   +   +  +SGI SW WNL G  STYHAL+PR+WT+Y+GEPDPEL++ 
Sbjct: 144 -KKYSSVLCPRNPKLDKQDSESGIGSWDWNLKGDKSTYHALYPRSWTMYEGEPDPELRIV 203

Query: 211 CRQISPFIPHNYRDSSLPTAVFVYTLVNTGKERAKVSLVFSWANSIGGISHLSGDHVNEP 270
           CRQ+SPFIPHNY++SS P +VF +TL N G   A V+L+F+WANS+GG S  SG H N  
Sbjct: 204 CRQVSPFIPHNYKESSFPVSVFTFTLHNLGNTTADVTLLFTWANSVGGDSEFSGGHYNSK 263

Query: 271 FIGEDGVSGVLLHHKTAKGNPPVTFAIAACETQNVSVTVLPSFGLS-EGSGVTAKDMWDK 330
               DGV GVLLHHKTA G P +++AI+A  T  VSV+  P F +S +  G+TAKDMW  
Sbjct: 264 ITMNDGVQGVLLHHKTANGLPSLSYAISAQATDGVSVSACPFFIVSGKQDGITAKDMWQA 323

Query: 331 MVQDGQFDRGNFSSGPSVPSSPGETLCAAVAASAWVEPHGKCTVAFSLSWSSPKVKFLKG 390
           + ++G FD    +S  S+ S  G ++ AAVAAS  V P     V FSL+W  P+V+F  G
Sbjct: 324 VKENGSFDHLK-ASEASMQSDHGSSIGAAVAASVTVLPGESRIVTFSLAWDCPEVQFPSG 383

Query: 391 FSYHRRYTKFYGTSEKAAQTLAHDALTNYKRWEEEIEKWQRPILMDERLPEWYKFTLFNE 450
             Y RRYTKFYG +  AA  +AHDA+  + +WE  IE WQRPIL D+RLP WY  TLFNE
Sbjct: 384 KIYSRRYTKFYGNNGDAAAQIAHDAILGHSQWESWIEDWQRPILEDKRLPAWYPVTLFNE 443

Query: 451 LYFLVAGGTVWIDSSVVGKKMANDHDELVARIKEDDIKATEAKVSGRGEEVSRTTTTTTL 510
           LY+L +GGT+W D S     +A         ++E      ++++  +             
Sbjct: 444 LYYLNSGGTLWTDGSSPVHSLAG--------VREKKFSLDKSQLGLK------------- 503

Query: 511 NDFSGVEFDDEENSTSSSHVSEDELMVPLKSGYTDRSYQTYKVMDPGNTEEDVGRFLYLE 570
           ND      +D   S      S  E    L +  T  S    K+++ G  EE++G FLYLE
Sbjct: 504 NDIDVPHQNDTAVSVLEKMASTLE---ELHASTTSNSAFGTKLLEEG--EENIGHFLYLE 563

Query: 571 GVEYVMWCTYDVHFYASFALLELFPKIELNIQRDFAKAVLSEDGRKVKFLADGKYGIRKV 630
           G+EY MW TYDVHFYASFAL+ LFPK+EL+IQRDFA AV+  D  KVK L++G++  RKV
Sbjct: 564 GIEYRMWNTYDVHFYASFALVMLFPKLELSIQRDFAAAVMLHDPTKVKTLSEGQWVQRKV 623

Query: 631 RGAVPHDLGTHDPWNEMNAYNIHDTSRWKDLNTKFVLQVYRDFAATRDMSFGVDVWPSVR 690
            GAVPHDLG +DPW E+N Y +H+T RWKDLN KFVLQVYRD  AT D  F   VWPSV 
Sbjct: 624 LGAVPHDLGINDPWFEVNGYTLHNTDRWKDLNPKFVLQVYRDVVATGDKKFASAVWPSVY 683

Query: 691 AAIEYMEQFDRDGDGVIENDGFPDQTYDTWTVHGISAYCGCLWVAALQAAAAMAHELGDQ 750
            A+ YM QFD+DGDG+IEN+GFPDQTYDTW+  G+SAYCG LWVAALQAA+A+A  +GD+
Sbjct: 684 VAMAYMAQFDKDGDGMIENEGFPDQTYDTWSASGVSAYCGGLWVAALQAASALARVVGDK 743

Query: 751 EFAETCKSKFLKAKPVLEAKLWNGSYFNYDSGSSSNSKSIQADQLAGQWYTASSGLPPLF 810
              +   SKF KAK V E KLWNGSYFNYD+  S  S +IQADQLAGQWY  +SGL P+ 
Sbjct: 744 NSQDYFWSKFQKAKVVYEKKLWNGSYFNYDNSGSQYSSTIQADQLAGQWYARASGLLPIV 803

Query: 811 DDFKIKSALKKIYDFNVMKIRGGRMGAVNGMHPNGKVDETCMQSREIWTGVTYGVAATMI 870
           D+ K ++AL+K+Y++NVMKI+ G+ GAVNGMHPNGKVD   MQSREIW+GVTY ++ATMI
Sbjct: 804 DEDKARTALEKVYNYNVMKIKDGKRGAVNGMHPNGKVDTASMQSREIWSGVTYALSATMI 863

Query: 871 LAGMEEEAFKTAEGIFLAGWSEEGFGYWFQTPEAWSTDGHYRSLIYMRPLSIWGMQWALS 930
             G+ E AF+TA GI+ A WSE G GY FQTPE+W+T   YRSL YMRPL+IW MQWAL+
Sbjct: 864 QEGLVEMAFQTASGIYEAAWSETGLGYSFQTPESWNTVDEYRSLTYMRPLAIWAMQWALT 893

BLAST of CmaCh08G010320 vs. TAIR 10
Match: AT1G33700.1 (Beta-glucosidase, GBA2 type family protein )

HSP 1 Score: 898.7 bits (2321), Expect = 4.3e-261
Identity = 464/942 (49.26%), Postives = 621/942 (65.92%), Query Frame = 0

Query: 36  PEQAWRRKLNGHANLLKEFSVTFVEAIKMVRLGIRLWSYVREEASQGRKAPIDPFTKEAC 95
           P  +W RKLN  A    EF ++  + + +  LG RLW + ++EA++GR +  D F K   
Sbjct: 18  PPFSWERKLNSQAKTPSEFKLSKRDHMHLFPLGYRLWRHTKDEAAKGRASIFDIFRKHHI 77

Query: 96  KPSASQGVPLGGMGSGSISRGFRGEFRQWQIIPGTCEASPVMANQFSIFISRDGGNKKYA 155
             +   GVPLGG+GSGSI R ++GEF+Q+++ P  CE +P++ NQFS F+SR GG  K++
Sbjct: 78  --TGDHGVPLGGIGSGSIGRSYKGEFQQFKLFPKICEEAPILTNQFSAFVSRPGG-VKHS 137

Query: 156 SVLAPGQHEGI---------GKAGDSGISSWGWNLDGQHSTYHALFPRAWTVYDGEPDPE 215
           +VL P + + +         G+A + GI SW WN+ G+ STYHAL+PR+WTVYDGEPDPE
Sbjct: 138 TVLCPSKPQVVKDNGGYLCQGQAPNMGIESWDWNMTGEKSTYHALYPRSWTVYDGEPDPE 197

Query: 216 LKVSCRQISPFIPHNYRDSSLPTAVFVYTLVNTGKERAKVSLVFSWANSIGGISHLSGDH 275
           L++  RQ+SPFIPHNY +SSLP +VF +T+ NTG E A V+L+F+W NS+GG S L+G H
Sbjct: 198 LRIVSRQVSPFIPHNYEESSLPVSVFNFTVTNTGAEPAIVTLLFTWENSVGGASGLTGQH 257

Query: 276 VNEPFIGEDGVSGVLLHHKTAKGNPPVTFAIAACETQNVSVTVLPSFGLS--EGSGVTAK 335
            N     +DGV  V L HKTA G+PPV++AIAA ET++V V+  P F +S    + +TA 
Sbjct: 258 FNSTMKAKDGVHAVALQHKTANGHPPVSYAIAAKETEDVRVSSCPCFIVSGTTPNQITAG 317

Query: 336 DMWDKMVQDGQFDRGNFSSGPSVPSSPGETLCAAVAASAWVEPHGKCTVAFSLSWSSPKV 395
           DMWD++ ++  FD+   +S    PS PG ++ AA+AA   V P    TV FSLSW  P+ 
Sbjct: 318 DMWDEIKKNASFDK--LTSNACSPSKPGTSIGAAIAAKVKVPPGCDRTVTFSLSWDCPEA 377

Query: 396 KFLKGFSYHRRYTKFYGTSEKAAQTLAHDALTNYKRWEEEIEKWQRPILMDERLPEWYKF 455
           +F +  +YHRRYT+FYG+   AA  +AHDAL N+  WE +IE+WQ P+L D  LPEWY+ 
Sbjct: 378 RFDEK-TYHRRYTRFYGSLGNAAVAMAHDALLNFPEWETQIEEWQAPVLADTTLPEWYRV 437

Query: 456 TLFNELYFLVAGGTVWIDSSVVGKKMANDHDELVARIKEDDIKATEAKVSGRGEEVSRTT 515
           TLFNELY+  +GGT+W D              L  +   D I        GR  ++S   
Sbjct: 438 TLFNELYYFNSGGTMWTDG-------------LPPKQSLDSI--------GR-RKISLGL 497

Query: 516 TTTTLNDFSGVEFDDEENSTSSSHVSEDELMVPLKSGYTDRSYQTYKVMDPGNTEEDVGR 575
           +T   ND       D+ N         D +   + +  T  +     ++   NT E++G+
Sbjct: 498 STIDKND------QDQNNVALDILGRIDAVCSQIHAPLTSNAALGNTMIQ--NTTENIGQ 557

Query: 576 FLYLEGVEYVMWCTYDVHFYASFALLELFPKIELNIQRDFAKAVLSEDGRKVKFLADGKY 635
           FLYLEGV+Y+M+ TYDVHFY+SFALL LFPKIEL+IQRDFA AVL  D  K + ++ G++
Sbjct: 558 FLYLEGVQYLMYNTYDVHFYSSFALLMLFPKIELSIQRDFAAAVLMHDSSKKQVMSSGEF 617

Query: 636 GIRKVRGAVPHDLGTHDPWNEMNAYNIHDTSRWKDLNTKFVLQVYRDFAATRDMSFGVDV 695
             RKV GAVPHD+G +DPW E+NAYN+ +T RWKDLN+KFVLQVYRD  AT D++F   V
Sbjct: 618 VTRKVLGAVPHDIGLNDPWFELNAYNLFNTDRWKDLNSKFVLQVYRDVVATGDLNFAKAV 677

Query: 696 WPSVRAAIEYMEQFDRDGDGVIENDGFPDQTYDTWTVHGISAYCGCLWVAALQAAAAMAH 755
           WPSV  AI Y++QFD+DGDG+IEN+GFPDQTYD W+  G+SAYCG LWVAALQA +A+A 
Sbjct: 678 WPSVYTAIAYLDQFDKDGDGMIENEGFPDQTYDAWSCSGVSAYCGGLWVAALQAGSALAR 737

Query: 756 ELGDQEFAETCKSKFLKAKPVLEAKLWNGSYFNYDSGSSSNSKSIQADQLAGQWYTASSG 815
           E+GD   A    +K+ KA+ V E KLWNGSYFNYD+  S +S SI ADQ+AGQWY  + G
Sbjct: 738 EIGDNGAAVYFNAKYEKARSVYE-KLWNGSYFNYDNSRSGSSSSILADQMAGQWYARACG 797

Query: 816 LPPLFDDFKIKSALKKIYDFNVMKIRGGRMGAVNGMHPNGKVDETCMQSREIWTGVTYGV 875
           L P+  +  IK AL+ +YDFNVM++R G  GAVNGM P+G+VD + M SRE+W G TY V
Sbjct: 798 LKPIAKEEWIKKALETVYDFNVMRVRDGTRGAVNGMLPDGRVDTSTMVSREVWAGTTYSV 857

Query: 876 AATMILAGMEEEAFKTAEGIFLAGWSEEGFGYWFQTPEAWSTDGHYRSLIYMRPLSIWGM 935
           AA MI  G+ ++ F+TA GI+ A WS+ G G  FQTPEAW+T+  YRSL YMRPL+IWG+
Sbjct: 858 AACMIQEGLADKGFRTASGIYEAAWSDRGLGCAFQTPEAWTTNDEYRSLCYMRPLAIWGI 917

Query: 936 QWALSLPKAILEAPKINVMDRIHVSSASTKFFHHETGVRRIA 967
           QWA ++PK   E     V  R     A++  F    G  ++A
Sbjct: 918 QWAHTMPKPNREQ---EVSLRPQEEDATSVLFQQHAGFIKVA 919

BLAST of CmaCh08G010320 vs. TAIR 10
Match: AT1G33700.2 (Beta-glucosidase, GBA2 type family protein )

HSP 1 Score: 898.7 bits (2321), Expect = 4.3e-261
Identity = 464/942 (49.26%), Postives = 621/942 (65.92%), Query Frame = 0

Query: 36  PEQAWRRKLNGHANLLKEFSVTFVEAIKMVRLGIRLWSYVREEASQGRKAPIDPFTKEAC 95
           P  +W RKLN  A    EF ++  + + +  LG RLW + ++EA++GR +  D F K   
Sbjct: 18  PPFSWERKLNSQAKTPSEFKLSKRDHMHLFPLGYRLWRHTKDEAAKGRASIFDIFRKHHI 77

Query: 96  KPSASQGVPLGGMGSGSISRGFRGEFRQWQIIPGTCEASPVMANQFSIFISRDGGNKKYA 155
             +   GVPLGG+GSGSI R ++GEF+Q+++ P  CE +P++ NQFS F+SR GG  K++
Sbjct: 78  --TGDHGVPLGGIGSGSIGRSYKGEFQQFKLFPKICEEAPILTNQFSAFVSRPGG-VKHS 137

Query: 156 SVLAPGQHEGI---------GKAGDSGISSWGWNLDGQHSTYHALFPRAWTVYDGEPDPE 215
           +VL P + + +         G+A + GI SW WN+ G+ STYHAL+PR+WTVYDGEPDPE
Sbjct: 138 TVLCPSKPQVVKDNGGYLCQGQAPNMGIESWDWNMTGEKSTYHALYPRSWTVYDGEPDPE 197

Query: 216 LKVSCRQISPFIPHNYRDSSLPTAVFVYTLVNTGKERAKVSLVFSWANSIGGISHLSGDH 275
           L++  RQ+SPFIPHNY +SSLP +VF +T+ NTG E A V+L+F+W NS+GG S L+G H
Sbjct: 198 LRIVSRQVSPFIPHNYEESSLPVSVFNFTVTNTGAEPAIVTLLFTWENSVGGASGLTGQH 257

Query: 276 VNEPFIGEDGVSGVLLHHKTAKGNPPVTFAIAACETQNVSVTVLPSFGLS--EGSGVTAK 335
            N     +DGV  V L HKTA G+PPV++AIAA ET++V V+  P F +S    + +TA 
Sbjct: 258 FNSTMKAKDGVHAVALQHKTANGHPPVSYAIAAKETEDVRVSSCPCFIVSGTTPNQITAG 317

Query: 336 DMWDKMVQDGQFDRGNFSSGPSVPSSPGETLCAAVAASAWVEPHGKCTVAFSLSWSSPKV 395
           DMWD++ ++  FD+   +S    PS PG ++ AA+AA   V P    TV FSLSW  P+ 
Sbjct: 318 DMWDEIKKNASFDK--LTSNACSPSKPGTSIGAAIAAKVKVPPGCDRTVTFSLSWDCPEA 377

Query: 396 KFLKGFSYHRRYTKFYGTSEKAAQTLAHDALTNYKRWEEEIEKWQRPILMDERLPEWYKF 455
           +F +  +YHRRYT+FYG+   AA  +AHDAL N+  WE +IE+WQ P+L D  LPEWY+ 
Sbjct: 378 RFDEK-TYHRRYTRFYGSLGNAAVAMAHDALLNFPEWETQIEEWQAPVLADTTLPEWYRV 437

Query: 456 TLFNELYFLVAGGTVWIDSSVVGKKMANDHDELVARIKEDDIKATEAKVSGRGEEVSRTT 515
           TLFNELY+  +GGT+W D              L  +   D I        GR  ++S   
Sbjct: 438 TLFNELYYFNSGGTMWTDG-------------LPPKQSLDSI--------GR-RKISLGL 497

Query: 516 TTTTLNDFSGVEFDDEENSTSSSHVSEDELMVPLKSGYTDRSYQTYKVMDPGNTEEDVGR 575
           +T   ND       D+ N         D +   + +  T  +     ++   NT E++G+
Sbjct: 498 STIDKND------QDQNNVALDILGRIDAVCSQIHAPLTSNAALGNTMIQ--NTTENIGQ 557

Query: 576 FLYLEGVEYVMWCTYDVHFYASFALLELFPKIELNIQRDFAKAVLSEDGRKVKFLADGKY 635
           FLYLEGV+Y+M+ TYDVHFY+SFALL LFPKIEL+IQRDFA AVL  D  K + ++ G++
Sbjct: 558 FLYLEGVQYLMYNTYDVHFYSSFALLMLFPKIELSIQRDFAAAVLMHDSSKKQVMSSGEF 617

Query: 636 GIRKVRGAVPHDLGTHDPWNEMNAYNIHDTSRWKDLNTKFVLQVYRDFAATRDMSFGVDV 695
             RKV GAVPHD+G +DPW E+NAYN+ +T RWKDLN+KFVLQVYRD  AT D++F   V
Sbjct: 618 VTRKVLGAVPHDIGLNDPWFELNAYNLFNTDRWKDLNSKFVLQVYRDVVATGDLNFAKAV 677

Query: 696 WPSVRAAIEYMEQFDRDGDGVIENDGFPDQTYDTWTVHGISAYCGCLWVAALQAAAAMAH 755
           WPSV  AI Y++QFD+DGDG+IEN+GFPDQTYD W+  G+SAYCG LWVAALQA +A+A 
Sbjct: 678 WPSVYTAIAYLDQFDKDGDGMIENEGFPDQTYDAWSCSGVSAYCGGLWVAALQAGSALAR 737

Query: 756 ELGDQEFAETCKSKFLKAKPVLEAKLWNGSYFNYDSGSSSNSKSIQADQLAGQWYTASSG 815
           E+GD   A    +K+ KA+ V E KLWNGSYFNYD+  S +S SI ADQ+AGQWY  + G
Sbjct: 738 EIGDNGAAVYFNAKYEKARSVYE-KLWNGSYFNYDNSRSGSSSSILADQMAGQWYARACG 797

Query: 816 LPPLFDDFKIKSALKKIYDFNVMKIRGGRMGAVNGMHPNGKVDETCMQSREIWTGVTYGV 875
           L P+  +  IK AL+ +YDFNVM++R G  GAVNGM P+G+VD + M SRE+W G TY V
Sbjct: 798 LKPIAKEEWIKKALETVYDFNVMRVRDGTRGAVNGMLPDGRVDTSTMVSREVWAGTTYSV 857

Query: 876 AATMILAGMEEEAFKTAEGIFLAGWSEEGFGYWFQTPEAWSTDGHYRSLIYMRPLSIWGM 935
           AA MI  G+ ++ F+TA GI+ A WS+ G G  FQTPEAW+T+  YRSL YMRPL+IWG+
Sbjct: 858 AACMIQEGLADKGFRTASGIYEAAWSDRGLGCAFQTPEAWTTNDEYRSLCYMRPLAIWGI 917

Query: 936 QWALSLPKAILEAPKINVMDRIHVSSASTKFFHHETGVRRIA 967
           QWA ++PK   E     V  R     A++  F    G  ++A
Sbjct: 918 QWAHTMPKPNREQ---EVSLRPQEEDATSVLFQQHAGFIKVA 919

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q5M8682.6e-17841.02Non-lysosomal glucosylceramidase OS=Rattus norvegicus OX=10116 GN=Gba2 PE=2 SV=2[more]
Q9HCG74.1e-17640.90Non-lysosomal glucosylceramidase OS=Homo sapiens OX=9606 GN=GBA2 PE=1 SV=2[more]
Q69ZF39.2e-17640.68Non-lysosomal glucosylceramidase OS=Mus musculus OX=10090 GN=Gba2 PE=1 SV=2[more]
Q7KT914.2e-13633.44Non-lysosomal glucosylceramidase OS=Drosophila melanogaster OX=7227 GN=CG33090 P... [more]
Match NameE-valueIdentityDescription
A0A6J1KIS90.0e+00100.00Non-lysosomal glucosylceramidase OS=Cucurbita maxima OX=3661 GN=LOC111494347 PE=... [more]
A0A6J1HKE80.0e+0099.39Non-lysosomal glucosylceramidase OS=Cucurbita moschata OX=3662 GN=LOC111464357 P... [more]
A0A0A0LK880.0e+0094.60Non-lysosomal glucosylceramidase OS=Cucumis sativus OX=3659 GN=Csa_2G130670 PE=3... [more]
A0A5D3CTQ80.0e+0094.50Non-lysosomal glucosylceramidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676... [more]
A0A1S3C4N30.0e+0094.50Non-lysosomal glucosylceramidase OS=Cucumis melo OX=3656 GN=LOC103496976 PE=3 SV... [more]
Match NameE-valueIdentityDescription
XP_023000039.10.0e+00100.00non-lysosomal glucosylceramidase [Cucurbita maxima][more]
KAG6593789.10.0e+0099.49Non-lysosomal glucosylceramidase, partial [Cucurbita argyrosperma subsp. sororia... [more]
XP_022964300.10.0e+0099.39non-lysosomal glucosylceramidase [Cucurbita moschata][more]
XP_023514140.10.0e+0099.29non-lysosomal glucosylceramidase [Cucurbita pepo subsp. pepo] >XP_023514141.1 no... [more]
KAG7026118.10.0e+0096.73Non-lysosomal glucosylceramidase, partial [Cucurbita argyrosperma subsp. argyros... [more]
Match NameE-valueIdentityDescription
AT3G24180.10.0e+0075.18Beta-glucosidase, GBA2 type family protein [more]
AT3G24180.20.0e+0075.18Beta-glucosidase, GBA2 type family protein [more]
AT5G49900.15.5e-27753.78Beta-glucosidase, GBA2 type family protein [more]
AT1G33700.14.3e-26149.26Beta-glucosidase, GBA2 type family protein [more]
AT1G33700.24.3e-26149.26Beta-glucosidase, GBA2 type family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR024462Glycosyl-hydrolase family 116, N-terminalPFAMPF12215Glyco_hydr_116Ncoord: 102..421
e-value: 2.9E-110
score: 368.6
IPR006775Glycosyl-hydrolase family 116, catalytic regionPFAMPF04685DUF608coord: 563..924
e-value: 9.6E-157
score: 521.8
IPR012341Six-hairpin glycosidase-like superfamilyGENE3D1.50.10.10coord: 609..924
e-value: 3.7E-22
score: 81.2
IPR014551Beta-glucosidase GBA2-typePIRSFPIRSF028944Beta_gluc_GBA2coord: 33..934
e-value: 0.0
score: 1048.5
NoneNo IPR availablePANTHERPTHR12654BILE ACID BETA-GLUCOSIDASE-RELATEDcoord: 1..527
coord: 554..981
NoneNo IPR availablePANTHERPTHR12654:SF28NON-LYSOSOMAL GLUCOSYLCERAMIDASEcoord: 1..527
coord: 554..981
IPR008928Six-hairpin glycosidase superfamilySUPERFAMILY48208Six-hairpin glycosidasescoord: 575..929

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh08G010320.1CmaCh08G010320.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
biological_process GO:0006680 glucosylceramide catabolic process
cellular_component GO:0016020 membrane
molecular_function GO:0008422 beta-glucosidase activity
molecular_function GO:0004348 glucosylceramidase activity
molecular_function GO:0004553 hydrolase activity, hydrolyzing O-glycosyl compounds