Homology
BLAST of CmaCh08G010320 vs. ExPASy Swiss-Prot
Match:
Q5M868 (Non-lysosomal glucosylceramidase OS=Rattus norvegicus OX=10116 GN=Gba2 PE=2 SV=2)
HSP 1 Score: 627.5 bits (1617), Expect = 2.6e-178
Identity = 363/885 (41.02%), Postives = 480/885 (54.24%), Query Frame = 0
Query: 55 SVTFVEAIKMVRLGIRLWSYVREEASQGRKAP-IDPFTKEACKPSASQGVPLGGMGSGSI 114
+V+ +K +G+R + + +K P ID F + G PLGG+G G+I
Sbjct: 96 NVSLSNLVKHFGMGLRYLKWWYRKTQVEKKTPFIDMFNSVPLR--QIYGCPLGGIGGGTI 155
Query: 115 SRGFRGEFRQWQIIPGTCEASPVMANQFSIFISRDGGNKKYASVLAPGQHEGIGKAGDSG 174
+RG+RG+F +WQ+ PG + V+A+QF + + RD G Y VL+ S
Sbjct: 156 TRGWRGQFCRWQLNPGMYQHQTVIADQFIVCLRRD-GRTVYQQVLS--------LELPSV 215
Query: 175 ISSWGWNLDGQHSTYHALFPRAWTVYDGEPDPELKVSCRQISPFIPHNYRDSSLPTAVFV 234
+ SW W L G + YHAL+PRAWTVY P + ++CRQI+P +PH+Y+DSSLP VFV
Sbjct: 216 LRSWNWGLCGYFAFYHALYPRAWTVYQ-LPGQNVTLTCRQITPILPHDYQDSSLPVGVFV 275
Query: 235 YTLVNTGKERAKVSLVFSWANSIGGISHLSGDHVNEPF-IGEDG--VSGVLLHHKTAKGN 294
+ + N G E VS++FS N +GG +G NEPF + +DG V G+LLHH T
Sbjct: 276 WDVENEGDETLDVSIMFSMRNGLGGEDDAAGGLWNEPFRLEQDGTTVQGLLLHHPTPPN- 335
Query: 295 PPVTFAIAACETQNVSVTVLPSFGLSEGSGVTAKDMWDKMVQDGQFDRGNFSSGPSVPSS 354
P T A+AA T + +VT +F T + +W ++QDGQ D +G S P+
Sbjct: 336 -PYTMAVAARHTADTTVTYTTAFDPDS----TGQQVWQDLLQDGQLDS---PAGQSTPTQ 395
Query: 355 PGETLCAAVAASAWVEPHGKCTVAFSLSWSSPKVKF-LKGFSYHRRYTKFYGTSEKAAQT 414
GE + AV AS+ + P G+C + FSL+W P++ F KG ++RRYT+F+G+ A
Sbjct: 396 RGEGVAGAVCASSKLLPRGRCCLEFSLAWDMPRIMFGAKGQVHYRRYTRFFGSDGDVAPA 455
Query: 415 LAHDALTNYKRWEEEIEKWQRPILMDERLPEWYKFTLFNELYFLVAGGTVWIDSSVVGKK 474
L+H AL Y WE I WQ P+L D LP WYK LFNELYFL GGTVW++
Sbjct: 456 LSHYALCQYAGWENSISAWQNPVLDDRSLPAWYKSALFNELYFLADGGTVWLE------- 515
Query: 475 MANDHDELVARIKEDDIKATEAKVSGRGEEVSRTTTTTTLNDFSGVEFDDEENSTSSSHV 534
V
Sbjct: 516 -----------------------------------------------------------V 575
Query: 535 SEDELMVPLKSGYTDRSYQTYKVMDPGNTEEDVGRFLYLEGVEYVMWCTYDVHFYASFAL 594
ED L L YQ ++ +D GRF YLEG EY M+ TYDVHFYASFAL
Sbjct: 576 PEDSLPEELGGS----MYQLRPIL------QDYGRFGYLEGQEYRMYNTYDVHFYASFAL 635
Query: 595 LELFPKIELNIQRDFAKAVLSEDGRKVKFLADGKYGIRKVRGAVPHDLGTHD--PWNEMN 654
+ L+PK+EL++Q D A A ED + ++L G K R +PHD+G D PW +N
Sbjct: 636 VMLWPKLELSLQYDMALATFKEDLTRRRYLMSGVVAPVKRRNVIPHDIGDPDDEPWLRVN 695
Query: 655 AYNIHDTSRWKDLNTKFVLQVYRDFAATRDMSFGVDVWPSVRAAIEYMEQFDRDGDGVIE 714
AY IHDT+ WKDLN KFVLQVYRD+ T D F D+WP A +E +FD+D DG+IE
Sbjct: 696 AYLIHDTADWKDLNLKFVLQVYRDYYLTGDQGFLKDMWPVCLAVMESEMKFDKDQDGLIE 755
Query: 715 NDGFPDQTYDTWTVHGISAYCGCLWVAALQAAAAMAHELGDQEFAETCKSKFLKAKPVLE 774
N G+ DQTYD W G SAYCG LW+AA+ MA G Q+ + S + + E
Sbjct: 756 NGGYADQTYDGWVTTGPSAYCGGLWLAAVAVMVQMAVLCGAQDVQDKFSSILCRGREAYE 815
Query: 775 AKLWNGSYFNYDSGSSSNSKSIQADQLAGQWYTASSGL----PPLFDDFKIKSALKKIYD 834
LWNG Y+NYDS S S+S+ +DQ AGQW+ + GL +F + ALK I++
Sbjct: 816 RLLWNGRYYNYDSSSQPQSRSVMSDQCAGQWFLRACGLGEGDTEVFPTLHVVRALKTIFE 875
Query: 835 FNVMKIRGGRMGAVNGMHPNGKVDETCMQSREIWTGVTYGVAATMILAGMEEEAFKTAEG 894
NV GG MGAVNGM P+G D + +QS E+W GV YG+AATMI G+ E F+TAEG
Sbjct: 876 LNVQAFAGGAMGAVNGMQPHGVPDRSSVQSDEVWVGVVYGLAATMIQEGLTWEGFRTAEG 881
Query: 895 IFLAGWSEEGFGYWFQTPEAWSTDGHYRSLIYMRPLSIWGMQWAL 929
+ W E G FQTPEA+ +RSL YMRPLSIW MQ AL
Sbjct: 936 CYRTVW--ERLGLAFQTPEAYCQQRVFRSLAYMRPLSIWAMQLAL 881
BLAST of CmaCh08G010320 vs. ExPASy Swiss-Prot
Match:
Q9HCG7 (Non-lysosomal glucosylceramidase OS=Homo sapiens OX=9606 GN=GBA2 PE=1 SV=2)
HSP 1 Score: 620.2 bits (1598), Expect = 4.1e-176
Identity = 362/885 (40.90%), Postives = 483/885 (54.58%), Query Frame = 0
Query: 55 SVTFVEAIKMVRLGIRLWSYVREEASQGRKAP-IDPFTKEACKPSASQGVPLGGMGSGSI 114
+V+ IK + +G+R + + +K P ID + G PLGG+G G+I
Sbjct: 105 NVSLSNMIKHIGMGLRYLQWWYRKTHVEKKTPFIDMINSVPLR--QIYGCPLGGIGGGTI 164
Query: 115 SRGFRGEFRQWQIIPGTCEASPVMANQFSIFISRDGGNKKYASVLAPGQHEGIGKAGDSG 174
+RG+RG+F +WQ+ PG + V+A+QF++ + R+ G Y VL+ + S
Sbjct: 165 TRGWRGQFCRWQLNPGMYQHRTVIADQFTVCLRRE-GQTVYQQVLSLER--------PSV 224
Query: 175 ISSWGWNLDGQHSTYHALFPRAWTVYDGEPDPELKVSCRQISPFIPHNYRDSSLPTAVFV 234
+ SW W L G + YHAL+PRAWTVY P + ++CRQI+P +PH+Y+DSSLP VFV
Sbjct: 225 LRSWNWGLCGYFAFYHALYPRAWTVYQ-LPGQNVTLTCRQITPILPHDYQDSSLPVGVFV 284
Query: 235 YTLVNTGKERAKVSLVFSWANSIGGISHLSGDHVNEPFIGE---DGVSGVLLHHKTAKGN 294
+ + N G E VS++FS N +GG G NEPF E + V G+LLHH T
Sbjct: 285 WDVENEGDEALDVSIMFSMRNGLGGGDDAPGGLWNEPFCLERSGETVRGLLLHHPTLPN- 344
Query: 295 PPVTFAIAACETQNVSVTVLPSFGLSEGSGVTAKDMWDKMVQDGQFDRGNFSSGPSVPSS 354
P T A+AA T +VT + +F T + +W ++QDGQ D +G S P+
Sbjct: 345 -PYTMAVAARVTAATTVTHITAFDPDS----TGQQVWQDLLQDGQLDS---PTGQSTPTQ 404
Query: 355 PGETLCAAVAASAWVEPHGKCTVAFSLSWSSPKVKF-LKGFSYHRRYTKFYGTSEKAAQT 414
G + AV S+ + P G+C + FSL+W P++ F KG ++RRYT+F+G AA
Sbjct: 405 KGVGIAGAVCVSSKLRPRGQCRLEFSLAWDMPRIMFGAKGQVHYRRYTRFFGQDGDAAPA 464
Query: 415 LAHDALTNYKRWEEEIEKWQRPILMDERLPEWYKFTLFNELYFLVAGGTVWIDSSVVGKK 474
L+H AL Y WEE I WQ P+L D LP WYK LFNELYFL GGTVW++
Sbjct: 465 LSHYALCRYAEWEERISAWQSPVLDDRSLPAWYKSALFNELYFLADGGTVWLE------- 524
Query: 475 MANDHDELVARIKEDDIKATEAKVSGRGEEVSRTTTTTTLNDFSGVEFDDEENSTSSSHV 534
+ ED + EE+ R
Sbjct: 525 -----------VLEDSLP----------EELGRN-------------------------- 584
Query: 535 SEDELMVPLKSGYTDRSYQTYKVMDPGNTEEDVGRFLYLEGVEYVMWCTYDVHFYASFAL 594
M L+ T D GRF YLEG EY M+ TYDVHFYASFAL
Sbjct: 585 -----MCHLRP-----------------TLRDYGRFGYLEGQEYRMYNTYDVHFYASFAL 644
Query: 595 LELFPKIELNIQRDFAKAVLSEDGRKVKFLADGKYGIRKVRGAVPHDLGTHD--PWNEMN 654
+ L+PK+EL++Q D A A L ED + ++L G K R +PHD+G D PW +N
Sbjct: 645 IMLWPKLELSLQYDMALATLREDLTRRRYLMSGVMAPVKRRNVIPHDIGDPDDEPWLRVN 704
Query: 655 AYNIHDTSRWKDLNTKFVLQVYRDFAATRDMSFGVDVWPSVRAAIEYMEQFDRDGDGVIE 714
AY IHDT+ WKDLN KFVLQVYRD+ T D +F D+WP A +E +FD+D DG+IE
Sbjct: 705 AYLIHDTADWKDLNLKFVLQVYRDYYLTGDQNFLKDMWPVCLAVMESEMKFDKDHDGLIE 764
Query: 715 NDGFPDQTYDTWTVHGISAYCGCLWVAALQAAAAMAHELGDQEFAETCKSKFLKAKPVLE 774
N G+ DQTYD W G SAYCG LW+AA+ MA G Q+ + S + + E
Sbjct: 765 NGGYADQTYDGWVTTGPSAYCGGLWLAAVAVMVQMAALCGAQDIQDKFSSILSRGQEAYE 824
Query: 775 AKLWNGSYFNYDSGSSSNSKSIQADQLAGQWYTASSGL----PPLFDDFKIKSALKKIYD 834
LWNG Y+NYDS S S+S+ +DQ AGQW+ + GL +F + AL+ I++
Sbjct: 825 RLLWNGRYYNYDSSSRPQSRSVMSDQCAGQWFLKACGLGEGDTEVFPTQHVVRALQTIFE 884
Query: 835 FNVMKIRGGRMGAVNGMHPNGKVDETCMQSREIWTGVTYGVAATMILAGMEEEAFKTAEG 894
NV GG MGAVNGM P+G D++ +QS E+W GV YG+AATMI G+ E F+TAEG
Sbjct: 885 LNVQAFAGGAMGAVNGMQPHGVPDKSSVQSDEVWVGVVYGLAATMIQEGLTWEGFQTAEG 890
Query: 895 IFLAGWSEEGFGYWFQTPEAWSTDGHYRSLIYMRPLSIWGMQWAL 929
+ W E G FQTPEA+ +RSL YMRPLSIW MQ AL
Sbjct: 945 CYRTVW--ERLGLAFQTPEAYCQQRVFRSLAYMRPLSIWAMQLAL 890
BLAST of CmaCh08G010320 vs. ExPASy Swiss-Prot
Match:
Q69ZF3 (Non-lysosomal glucosylceramidase OS=Mus musculus OX=10090 GN=Gba2 PE=1 SV=2)
HSP 1 Score: 619.0 bits (1595), Expect = 9.2e-176
Identity = 360/885 (40.68%), Postives = 482/885 (54.46%), Query Frame = 0
Query: 55 SVTFVEAIKMVRLGIRLWSYVREEASQGRKAP-IDPFTKEACKPSASQGVPLGGMGSGSI 114
+++ +K + +G+R + + +K P ID + G PLGG+G G+I
Sbjct: 96 NISLSNLVKHLGMGLRYLKWWYRKTHVEKKTPFIDMLNSLPLR--QIYGCPLGGIGGGTI 155
Query: 115 SRGFRGEFRQWQIIPGTCEASPVMANQFSIFISRDGGNKKYASVLAPGQHEGIGKAGDSG 174
+RG+RG+F +WQ+ PG + V+A+QF + + RD G Y VL+ +
Sbjct: 156 TRGWRGQFCRWQLNPGMYQHQTVIADQFIVCLRRD-GRTVYQQVLS--------LELPNV 215
Query: 175 ISSWGWNLDGQHSTYHALFPRAWTVYDGEPDPELKVSCRQISPFIPHNYRDSSLPTAVFV 234
+ SW W L G + YHAL+PRAWTVY P + ++CRQ++P +PH+Y+DSSLP VFV
Sbjct: 216 LRSWNWGLCGYFAFYHALYPRAWTVYQ-LPGQNVTLTCRQVTPILPHDYQDSSLPVGVFV 275
Query: 235 YTLVNTGKERAKVSLVFSWANSIGGISHLSGDHVNEPFIGEDG---VSGVLLHHKTAKGN 294
+ + N G E VS+ FS N +GG +G NEPF E G V G+LLHH T
Sbjct: 276 WDVENEGDETLDVSITFSMRNGLGGEDDAAGSLWNEPFRLEQGGTTVQGLLLHHPTPPN- 335
Query: 295 PPVTFAIAACETQNVSVTVLPSFGLSEGSGVTAKDMWDKMVQDGQFDRGNFSSGPSVPSS 354
P T A+AA T + +VT +F + +G T + +W ++QDGQ D +G S P+
Sbjct: 336 -PYTMAVAARCTADTTVTHTTAF---DPNG-TGQQVWQDLLQDGQLDS---PAGQSTPTQ 395
Query: 355 PGETLCAAVAASAWVEPHGKCTVAFSLSWSSPKVKF-LKGFSYHRRYTKFYGTSEKAAQT 414
GE + AV S+ + P +C + FSL+W PK+ F K ++RRYT+F+G+ A
Sbjct: 396 KGEGIAGAVCVSSKLLPRSRCCLEFSLAWDMPKIMFGAKSQVHYRRYTRFFGSDGDVAPA 455
Query: 415 LAHDALTNYKRWEEEIEKWQRPILMDERLPEWYKFTLFNELYFLVAGGTVWIDSSVVGKK 474
L+H AL +Y WE+ I WQ P+L D LP WYK LFNELYFL GGTVW++
Sbjct: 456 LSHYALCHYADWEDRISAWQNPVLDDRTLPAWYKSALFNELYFLADGGTVWLE------- 515
Query: 475 MANDHDELVARIKEDDIKATEAKVSGRGEEVSRTTTTTTLNDFSGVEFDDEENSTSSSHV 534
V
Sbjct: 516 -----------------------------------------------------------V 575
Query: 535 SEDELMVPLKSGYTDRSYQTYKVMDPGNTEEDVGRFLYLEGVEYVMWCTYDVHFYASFAL 594
D L P G + R + +T +D GRF YLEG EY M+ TYDVHFYASFAL
Sbjct: 576 PADSL--PEGLGGSMRQLR--------STLQDYGRFGYLEGQEYRMYNTYDVHFYASFAL 635
Query: 595 LELFPKIELNIQRDFAKAVLSEDGRKVKFLADGKYGIRKVRGAVPHDLGTHD--PWNEMN 654
+ L+PK+EL++Q D A A L ED + ++L G K R +PHD+G D PW +N
Sbjct: 636 VMLWPKLELSLQYDMALATLKEDLTRRRYLMSGVVAPVKRRNVIPHDIGDPDDEPWLRVN 695
Query: 655 AYNIHDTSRWKDLNTKFVLQVYRDFAATRDMSFGVDVWPSVRAAIEYMEQFDRDGDGVIE 714
AY IHDT+ WKDLN KFVLQ+YRD+ T D F D+WP A +E +FD+D DG+IE
Sbjct: 696 AYLIHDTADWKDLNLKFVLQIYRDYYLTGDQGFLEDMWPVCLAVMESEMKFDKDQDGLIE 755
Query: 715 NDGFPDQTYDTWTVHGISAYCGCLWVAALQAAAAMAHELGDQEFAETCKSKFLKAKPVLE 774
N G+ DQTYD W G SAYCG LW+AA+ MA G Q+ E S + + E
Sbjct: 756 NGGYADQTYDAWVTTGPSAYCGGLWLAAVAVMVQMAVLCGAQDVQERFASILCRGREAYE 815
Query: 775 AKLWNGSYFNYDSGSSSNSKSIQADQLAGQWYTASSGL----PPLFDDFKIKSALKKIYD 834
LWNG Y+NYDS S S+SI +DQ AGQW+ + GL +F + AL+ I++
Sbjct: 816 RLLWNGRYYNYDSSSHPQSRSIMSDQCAGQWFLRACGLGEGDTEVFPTLHVVRALQTIFE 875
Query: 835 FNVMKIRGGRMGAVNGMHPNGKVDETCMQSREIWTGVTYGVAATMILAGMEEEAFKTAEG 894
NV GG MGAVNGMHP+G D + +QS E+W GV YG+AATMI G+ E F+TAEG
Sbjct: 876 LNVQAFAGGAMGAVNGMHPHGVPDRSSVQSDEVWVGVVYGLAATMIQEGLTWEGFRTAEG 881
Query: 895 IFLAGWSEEGFGYWFQTPEAWSTDGHYRSLIYMRPLSIWGMQWAL 929
+ W E G FQTPEA+ +RSL YMRPLSIW MQ AL
Sbjct: 936 CYRTVW--ERLGLAFQTPEAYCQQQVFRSLAYMRPLSIWAMQLAL 881
BLAST of CmaCh08G010320 vs. ExPASy Swiss-Prot
Match:
Q7KT91 (Non-lysosomal glucosylceramidase OS=Drosophila melanogaster OX=7227 GN=CG33090 PE=1 SV=1)
HSP 1 Score: 487.3 bits (1253), Expect = 4.2e-136
Identity = 324/969 (33.44%), Postives = 462/969 (47.68%), Query Frame = 0
Query: 60 EAIKMVRLGIRLWSYVREEASQGRKAPIDPFTKEACKPSASQGVPLGGMGSGSISRGFRG 119
+ + MV L R +Y + + +GR+ +D + E K GVP+GG+G G+I RG+ G
Sbjct: 77 QTLPMVPLVCRYAAYYWKVSREGRRVYMDYYYMENGK--QIYGVPIGGIGGGTIGRGYAG 136
Query: 120 EFRQWQIIPGTCEASPVMANQFSIFISRDGGNKKYASVLA-----------PGQHEG--- 179
EF ++Q+ PG E + V+ANQF + I G + S+L+ G +G
Sbjct: 137 EFCRFQMRPGIYEYNVVLANQFIVTIKDPKGCTIFQSLLSKCSTRDKTSDPDGDPDGERT 196
Query: 180 ------IGKAGDSGISSWGWNLDGQHSTYHALFPRAWTVYDGEPDPELKVSCRQISPFIP 239
+S+W N++ +Y L+PR+WT YD ++++CRQ+SP IP
Sbjct: 197 KCQLPNCSSRAKQPLSAWHSNIEDTRCSYTGLYPRSWTEYD-LSHYGVRLTCRQVSPVIP 256
Query: 240 HNYRDSSLPTAVFVYTLVNTGKERAKVSLVFSWANSIGGISHLSGDHVNEPFIGEDGVSG 299
H YR+SSLP AVFV+++ N + KVS+ F++ N G + I E G
Sbjct: 257 HEYRESSLPCAVFVWSVENVCDQERKVSITFTFKNGTGNKKQDAEGGAESQLISEGNAKG 316
Query: 300 VLLHHKTAKGNPPVTFAIAACETQNVSVTVLPSFGLSEGSGVTAKDMWDKMVQDGQFDRG 359
V + K ++ P ++ +A +S+T P F G+G + +W ++ + GQ
Sbjct: 317 VSIRQKISE--MPCSYNLACRVLPEISITRCPQFD-PAGNG---EQLWAQLKEHGQ---- 376
Query: 360 NFSSGPSVPSSPGETLCAAVAASAWVEPHGKCTVAFSLSWSSPKVKFLKGFSYHRR-YTK 419
S P+ + + + AV ++P + F L+W PK++F + H R YTK
Sbjct: 377 -LSEHPTSEALKTKDIGVAVCGQVALKPMASHDLEFVLAWDMPKIQFPRKMQTHTRYYTK 436
Query: 420 FYGTSEKAAQTLAHDALTNYKRWEEEIEKWQRPILMDERLPEWYKFTLFNELYFLVAGGT 479
++ S + + AL Y WE I+ WQRPIL DE LP+WYK +FN+LYF+ GGT
Sbjct: 437 YFDDSGDSGPRICEYALRQYSTWERLIDAWQRPILNDETLPDWYKCAIFNQLYFISDGGT 496
Query: 480 VWID-SSVVGKKMANDHDELVARIKEDDIKATEAKVSGRGEEVSRTTTTTTLNDFSGVEF 539
+W+ S +GK++A D L
Sbjct: 497 IWLKCDSSLGKELAYDDPRLA--------------------------------------- 556
Query: 540 DDEENSTSSSHVSEDELMVPLKSGYTDRSYQTYKVMDPGNTEEDVGRFLYLEGVEYVMWC 599
GRF YLEG EY M+
Sbjct: 557 --------------------------------------------YGRFGYLEGHEYRMYN 616
Query: 600 TYDVHFYASFALLELFPKIELNIQRDFAKAVLSEDGRKVKFLADGKYGIRKVRGAVPHDL 659
TYDVHFYAS AL L+P +++++Q DF A+ +E K L DGK RKV+ VPHDL
Sbjct: 617 TYDVHFYASPALAHLWPNLQVSLQYDFKDAIAAELNDTRKMLYDGKVMPRKVKNCVPHDL 676
Query: 660 GTHD--PWNEMNAYNIHDTSRWKDLNTKFVLQVYRDFAATRDMS---------------- 719
G D P+ +N YNIHD + WKDLNTKFVLQVYRD+ +++
Sbjct: 677 GDPDEEPFTLINCYNIHDVNDWKDLNTKFVLQVYRDYYVLNELAQAQSDNASKFSSIEFI 736
Query: 720 --------FGVD---------------------------------------VWPSVRAAI 779
+ D ++ S +A +
Sbjct: 737 DKESLYELYSQDNKRKNSADEKQQNRKSASMYINETNGKVYLMDAIGYLKAMYASCKAIM 796
Query: 780 EYMEQFDRDGDGVIENDGFPDQTYDTWTVHGISAYCGCLWVAALQAAAAMAHELGDQEFA 839
E ++D+D DG+IEN PDQTYD+W + G SAYC LW+AALQA +AMA L
Sbjct: 797 ERTIEYDKDNDGLIENTKMPDQTYDSWVMDGPSAYCSGLWLAALQAMSAMATILDQPNDC 856
Query: 840 ETCKSKFLKAKPVLEAKLWNGSYFNYDSGSSSNSKSIQADQLAGQWYTASSGLP-PLFDD 899
+ K K LE KLWNGSY+ +D S S+ +I ADQL G WY S G ++
Sbjct: 857 LRYQDILEKGKRSLEEKLWNGSYYRFDL-SHSHRDTIMADQLCGHWYLKSCGFDYEIYPK 916
Query: 900 FKIKSALKKIYDFNVMKIRGGRMGAVNGMHPN-------GKVDETCMQSREIWTGVTYGV 934
+++ALK+IYD NVM G +GA NG N G VD + +Q+ E+W GV Y +
Sbjct: 917 ENVRTALKRIYDNNVMGFHEGNIGAANGFIANASEPTKPGHVDNSNIQAEEVWPGVVYAL 945
BLAST of CmaCh08G010320 vs. ExPASy TrEMBL
Match:
A0A6J1KIS9 (Non-lysosomal glucosylceramidase OS=Cucurbita maxima OX=3661 GN=LOC111494347 PE=3 SV=1)
HSP 1 Score: 2026.1 bits (5248), Expect = 0.0e+00
Identity = 981/981 (100.00%), Postives = 981/981 (100.00%), Query Frame = 0
Query: 1 MVSGNLFHCRKNSWPPDEYISKSTLQLFDFDSASPPEQAWRRKLNGHANLLKEFSVTFVE 60
MVSGNLFHCRKNSWPPDEYISKSTLQLFDFDSASPPEQAWRRKLNGHANLLKEFSVTFVE
Sbjct: 1 MVSGNLFHCRKNSWPPDEYISKSTLQLFDFDSASPPEQAWRRKLNGHANLLKEFSVTFVE 60
Query: 61 AIKMVRLGIRLWSYVREEASQGRKAPIDPFTKEACKPSASQGVPLGGMGSGSISRGFRGE 120
AIKMVRLGIRLWSYVREEASQGRKAPIDPFTKEACKPSASQGVPLGGMGSGSISRGFRGE
Sbjct: 61 AIKMVRLGIRLWSYVREEASQGRKAPIDPFTKEACKPSASQGVPLGGMGSGSISRGFRGE 120
Query: 121 FRQWQIIPGTCEASPVMANQFSIFISRDGGNKKYASVLAPGQHEGIGKAGDSGISSWGWN 180
FRQWQIIPGTCEASPVMANQFSIFISRDGGNKKYASVLAPGQHEGIGKAGDSGISSWGWN
Sbjct: 121 FRQWQIIPGTCEASPVMANQFSIFISRDGGNKKYASVLAPGQHEGIGKAGDSGISSWGWN 180
Query: 181 LDGQHSTYHALFPRAWTVYDGEPDPELKVSCRQISPFIPHNYRDSSLPTAVFVYTLVNTG 240
LDGQHSTYHALFPRAWTVYDGEPDPELKVSCRQISPFIPHNYRDSSLPTAVFVYTLVNTG
Sbjct: 181 LDGQHSTYHALFPRAWTVYDGEPDPELKVSCRQISPFIPHNYRDSSLPTAVFVYTLVNTG 240
Query: 241 KERAKVSLVFSWANSIGGISHLSGDHVNEPFIGEDGVSGVLLHHKTAKGNPPVTFAIAAC 300
KERAKVSLVFSWANSIGGISHLSGDHVNEPFIGEDGVSGVLLHHKTAKGNPPVTFAIAAC
Sbjct: 241 KERAKVSLVFSWANSIGGISHLSGDHVNEPFIGEDGVSGVLLHHKTAKGNPPVTFAIAAC 300
Query: 301 ETQNVSVTVLPSFGLSEGSGVTAKDMWDKMVQDGQFDRGNFSSGPSVPSSPGETLCAAVA 360
ETQNVSVTVLPSFGLSEGSGVTAKDMWDKMVQDGQFDRGNFSSGPSVPSSPGETLCAAVA
Sbjct: 301 ETQNVSVTVLPSFGLSEGSGVTAKDMWDKMVQDGQFDRGNFSSGPSVPSSPGETLCAAVA 360
Query: 361 ASAWVEPHGKCTVAFSLSWSSPKVKFLKGFSYHRRYTKFYGTSEKAAQTLAHDALTNYKR 420
ASAWVEPHGKCTVAFSLSWSSPKVKFLKGFSYHRRYTKFYGTSEKAAQTLAHDALTNYKR
Sbjct: 361 ASAWVEPHGKCTVAFSLSWSSPKVKFLKGFSYHRRYTKFYGTSEKAAQTLAHDALTNYKR 420
Query: 421 WEEEIEKWQRPILMDERLPEWYKFTLFNELYFLVAGGTVWIDSSVVGKKMANDHDELVAR 480
WEEEIEKWQRPILMDERLPEWYKFTLFNELYFLVAGGTVWIDSSVVGKKMANDHDELVAR
Sbjct: 421 WEEEIEKWQRPILMDERLPEWYKFTLFNELYFLVAGGTVWIDSSVVGKKMANDHDELVAR 480
Query: 481 IKEDDIKATEAKVSGRGEEVSRTTTTTTLNDFSGVEFDDEENSTSSSHVSEDELMVPLKS 540
IKEDDIKATEAKVSGRGEEVSRTTTTTTLNDFSGVEFDDEENSTSSSHVSEDELMVPLKS
Sbjct: 481 IKEDDIKATEAKVSGRGEEVSRTTTTTTLNDFSGVEFDDEENSTSSSHVSEDELMVPLKS 540
Query: 541 GYTDRSYQTYKVMDPGNTEEDVGRFLYLEGVEYVMWCTYDVHFYASFALLELFPKIELNI 600
GYTDRSYQTYKVMDPGNTEEDVGRFLYLEGVEYVMWCTYDVHFYASFALLELFPKIELNI
Sbjct: 541 GYTDRSYQTYKVMDPGNTEEDVGRFLYLEGVEYVMWCTYDVHFYASFALLELFPKIELNI 600
Query: 601 QRDFAKAVLSEDGRKVKFLADGKYGIRKVRGAVPHDLGTHDPWNEMNAYNIHDTSRWKDL 660
QRDFAKAVLSEDGRKVKFLADGKYGIRKVRGAVPHDLGTHDPWNEMNAYNIHDTSRWKDL
Sbjct: 601 QRDFAKAVLSEDGRKVKFLADGKYGIRKVRGAVPHDLGTHDPWNEMNAYNIHDTSRWKDL 660
Query: 661 NTKFVLQVYRDFAATRDMSFGVDVWPSVRAAIEYMEQFDRDGDGVIENDGFPDQTYDTWT 720
NTKFVLQVYRDFAATRDMSFGVDVWPSVRAAIEYMEQFDRDGDGVIENDGFPDQTYDTWT
Sbjct: 661 NTKFVLQVYRDFAATRDMSFGVDVWPSVRAAIEYMEQFDRDGDGVIENDGFPDQTYDTWT 720
Query: 721 VHGISAYCGCLWVAALQAAAAMAHELGDQEFAETCKSKFLKAKPVLEAKLWNGSYFNYDS 780
VHGISAYCGCLWVAALQAAAAMAHELGDQEFAETCKSKFLKAKPVLEAKLWNGSYFNYDS
Sbjct: 721 VHGISAYCGCLWVAALQAAAAMAHELGDQEFAETCKSKFLKAKPVLEAKLWNGSYFNYDS 780
Query: 781 GSSSNSKSIQADQLAGQWYTASSGLPPLFDDFKIKSALKKIYDFNVMKIRGGRMGAVNGM 840
GSSSNSKSIQADQLAGQWYTASSGLPPLFDDFKIKSALKKIYDFNVMKIRGGRMGAVNGM
Sbjct: 781 GSSSNSKSIQADQLAGQWYTASSGLPPLFDDFKIKSALKKIYDFNVMKIRGGRMGAVNGM 840
Query: 841 HPNGKVDETCMQSREIWTGVTYGVAATMILAGMEEEAFKTAEGIFLAGWSEEGFGYWFQT 900
HPNGKVDETCMQSREIWTGVTYGVAATMILAGMEEEAFKTAEGIFLAGWSEEGFGYWFQT
Sbjct: 841 HPNGKVDETCMQSREIWTGVTYGVAATMILAGMEEEAFKTAEGIFLAGWSEEGFGYWFQT 900
Query: 901 PEAWSTDGHYRSLIYMRPLSIWGMQWALSLPKAILEAPKINVMDRIHVSSASTKFFHHET 960
PEAWSTDGHYRSLIYMRPLSIWGMQWALSLPKAILEAPKINVMDRIHVSSASTKFFHHET
Sbjct: 901 PEAWSTDGHYRSLIYMRPLSIWGMQWALSLPKAILEAPKINVMDRIHVSSASTKFFHHET 960
Query: 961 GVRRIATKAKCFGDSVFNCAC 982
GVRRIATKAKCFGDSVFNCAC
Sbjct: 961 GVRRIATKAKCFGDSVFNCAC 981
BLAST of CmaCh08G010320 vs. ExPASy TrEMBL
Match:
A0A6J1HKE8 (Non-lysosomal glucosylceramidase OS=Cucurbita moschata OX=3662 GN=LOC111464357 PE=3 SV=1)
HSP 1 Score: 2016.9 bits (5224), Expect = 0.0e+00
Identity = 975/981 (99.39%), Postives = 980/981 (99.90%), Query Frame = 0
Query: 1 MVSGNLFHCRKNSWPPDEYISKSTLQLFDFDSASPPEQAWRRKLNGHANLLKEFSVTFVE 60
MVSGNLFHCRKNSWPPDEYISKSTLQLFDFDSASPPEQAWRRKLNGHANLLKEFSVTFVE
Sbjct: 1 MVSGNLFHCRKNSWPPDEYISKSTLQLFDFDSASPPEQAWRRKLNGHANLLKEFSVTFVE 60
Query: 61 AIKMVRLGIRLWSYVREEASQGRKAPIDPFTKEACKPSASQGVPLGGMGSGSISRGFRGE 120
AIKMVRLGIRLWSYVREEASQGRKAPIDPFTKEACKPSASQGVPLGGMGSGSISRGFRGE
Sbjct: 61 AIKMVRLGIRLWSYVREEASQGRKAPIDPFTKEACKPSASQGVPLGGMGSGSISRGFRGE 120
Query: 121 FRQWQIIPGTCEASPVMANQFSIFISRDGGNKKYASVLAPGQHEGIGKAGDSGISSWGWN 180
FRQWQIIPGTCEASPVMANQFSIFISRDGGNKKYASVLAPGQHEGIGKAGDSGISSWGWN
Sbjct: 121 FRQWQIIPGTCEASPVMANQFSIFISRDGGNKKYASVLAPGQHEGIGKAGDSGISSWGWN 180
Query: 181 LDGQHSTYHALFPRAWTVYDGEPDPELKVSCRQISPFIPHNYRDSSLPTAVFVYTLVNTG 240
LDGQHSTYHALFPRAWTVYDGEPDPELKVSCRQISPFIPHNYRDSSLPTAVFVYTLVNTG
Sbjct: 181 LDGQHSTYHALFPRAWTVYDGEPDPELKVSCRQISPFIPHNYRDSSLPTAVFVYTLVNTG 240
Query: 241 KERAKVSLVFSWANSIGGISHLSGDHVNEPFIGEDGVSGVLLHHKTAKGNPPVTFAIAAC 300
+ERAKVSLVFSWANSIGGISHLSGDHVNEPFIGEDGVSGVLLHHKTAKGNPPVTFAIAAC
Sbjct: 241 RERAKVSLVFSWANSIGGISHLSGDHVNEPFIGEDGVSGVLLHHKTAKGNPPVTFAIAAC 300
Query: 301 ETQNVSVTVLPSFGLSEGSGVTAKDMWDKMVQDGQFDRGNFSSGPSVPSSPGETLCAAVA 360
ETQNVSVTVLPSFGLSEGSGVTAKDMWDKMVQDGQFDRGN+SSGPSVPSSPGETLCAAVA
Sbjct: 301 ETQNVSVTVLPSFGLSEGSGVTAKDMWDKMVQDGQFDRGNYSSGPSVPSSPGETLCAAVA 360
Query: 361 ASAWVEPHGKCTVAFSLSWSSPKVKFLKGFSYHRRYTKFYGTSEKAAQTLAHDALTNYKR 420
ASAWVEPHGKCTVAFSLSWSSPKVKFLKGFSYHRRYTKFYGTSEKAAQTLAHDALTNYKR
Sbjct: 361 ASAWVEPHGKCTVAFSLSWSSPKVKFLKGFSYHRRYTKFYGTSEKAAQTLAHDALTNYKR 420
Query: 421 WEEEIEKWQRPILMDERLPEWYKFTLFNELYFLVAGGTVWIDSSVVGKKMANDHDELVAR 480
WEEEIEKWQ PILMDERLPEWYKFTLFNELYFLVAGGTVWIDSSVVGKKMANDHDELVAR
Sbjct: 421 WEEEIEKWQGPILMDERLPEWYKFTLFNELYFLVAGGTVWIDSSVVGKKMANDHDELVAR 480
Query: 481 IKEDDIKATEAKVSGRGEEVSRTTTTTTLNDFSGVEFDDEENSTSSSHVSEDELMVPLKS 540
IKE+DIKATEA+VSGRGEEVSRTTTTTTLNDFSGVEFDDEENSTSSSHVSEDELMVPLKS
Sbjct: 481 IKEEDIKATEAEVSGRGEEVSRTTTTTTLNDFSGVEFDDEENSTSSSHVSEDELMVPLKS 540
Query: 541 GYTDRSYQTYKVMDPGNTEEDVGRFLYLEGVEYVMWCTYDVHFYASFALLELFPKIELNI 600
GYTDRSYQTYKVMDPGNTEEDVGRFLYLEGVEYVMWCTYDVHFYASFALLELFPKIELNI
Sbjct: 541 GYTDRSYQTYKVMDPGNTEEDVGRFLYLEGVEYVMWCTYDVHFYASFALLELFPKIELNI 600
Query: 601 QRDFAKAVLSEDGRKVKFLADGKYGIRKVRGAVPHDLGTHDPWNEMNAYNIHDTSRWKDL 660
QRDFAKAVLSEDGRKVKFLADGKYGIRKVRGAVPHDLGTHDPWNEMNAYNIHDTSRWKDL
Sbjct: 601 QRDFAKAVLSEDGRKVKFLADGKYGIRKVRGAVPHDLGTHDPWNEMNAYNIHDTSRWKDL 660
Query: 661 NTKFVLQVYRDFAATRDMSFGVDVWPSVRAAIEYMEQFDRDGDGVIENDGFPDQTYDTWT 720
NTKFVLQVYRDFAATRDMSFGVDVWPSVRAAIEYMEQFDRDGDGVIENDGFPDQTYDTWT
Sbjct: 661 NTKFVLQVYRDFAATRDMSFGVDVWPSVRAAIEYMEQFDRDGDGVIENDGFPDQTYDTWT 720
Query: 721 VHGISAYCGCLWVAALQAAAAMAHELGDQEFAETCKSKFLKAKPVLEAKLWNGSYFNYDS 780
VHGISAYCGCLWVAALQAAAAMAHELGDQEFAETCKSKFLKAKPVLEAKLWNGSYFNYDS
Sbjct: 721 VHGISAYCGCLWVAALQAAAAMAHELGDQEFAETCKSKFLKAKPVLEAKLWNGSYFNYDS 780
Query: 781 GSSSNSKSIQADQLAGQWYTASSGLPPLFDDFKIKSALKKIYDFNVMKIRGGRMGAVNGM 840
GSSSNSKSIQADQLAGQWYTASSGLPPLFDDFKIKSALKKIYDFNVMKI+GGRMGAVNGM
Sbjct: 781 GSSSNSKSIQADQLAGQWYTASSGLPPLFDDFKIKSALKKIYDFNVMKIKGGRMGAVNGM 840
Query: 841 HPNGKVDETCMQSREIWTGVTYGVAATMILAGMEEEAFKTAEGIFLAGWSEEGFGYWFQT 900
HPNGKVDETCMQSREIWTGVTYGVAATMILAGMEEEAFKTAEGIFLAGWSEEGFGYWFQT
Sbjct: 841 HPNGKVDETCMQSREIWTGVTYGVAATMILAGMEEEAFKTAEGIFLAGWSEEGFGYWFQT 900
Query: 901 PEAWSTDGHYRSLIYMRPLSIWGMQWALSLPKAILEAPKINVMDRIHVSSASTKFFHHET 960
PEAWSTDGHYRSLIYMRPLSIWGMQWALSLPKAILEAPKINVMDRIHVSSASTKFFHHET
Sbjct: 901 PEAWSTDGHYRSLIYMRPLSIWGMQWALSLPKAILEAPKINVMDRIHVSSASTKFFHHET 960
Query: 961 GVRRIATKAKCFGDSVFNCAC 982
GVRRIATKAKCFGDSVFNCAC
Sbjct: 961 GVRRIATKAKCFGDSVFNCAC 981
BLAST of CmaCh08G010320 vs. ExPASy TrEMBL
Match:
A0A0A0LK88 (Non-lysosomal glucosylceramidase OS=Cucumis sativus OX=3659 GN=Csa_2G130670 PE=3 SV=1)
HSP 1 Score: 1932.5 bits (5005), Expect = 0.0e+00
Identity = 928/981 (94.60%), Postives = 960/981 (97.86%), Query Frame = 0
Query: 1 MVSGNLFHCRKNSWPPDEYISKSTLQLFDFDSASPPEQAWRRKLNGHANLLKEFSVTFVE 60
MVSGNLFHCRKNSWPP+EYISKSTLQLFDFDSASPPEQAWRRKLNGHANLLKEFSVTFVE
Sbjct: 1 MVSGNLFHCRKNSWPPEEYISKSTLQLFDFDSASPPEQAWRRKLNGHANLLKEFSVTFVE 60
Query: 61 AIKMVRLGIRLWSYVREEASQGRKAPIDPFTKEACKPSASQGVPLGGMGSGSISRGFRGE 120
AIKMVRLGIRLWSYVREEASQGRKAPIDPFT+E+CKPSASQGVPLGGMGSGSISRGFRGE
Sbjct: 61 AIKMVRLGIRLWSYVREEASQGRKAPIDPFTRESCKPSASQGVPLGGMGSGSISRGFRGE 120
Query: 121 FRQWQIIPGTCEASPVMANQFSIFISRDGGNKKYASVLAPGQHEGIGKAGDSGISSWGWN 180
FRQWQIIPGTCEASPVMANQFSIF+SRDGG KKYASVLAPGQHEG+GK GDSGISSWGWN
Sbjct: 121 FRQWQIIPGTCEASPVMANQFSIFVSRDGGGKKYASVLAPGQHEGLGKDGDSGISSWGWN 180
Query: 181 LDGQHSTYHALFPRAWTVYDGEPDPELKVSCRQISPFIPHNYRDSSLPTAVFVYTLVNTG 240
LDGQHSTYHALFPRAWTVYDGEPDPELKVSCRQISPFIPHNYRDSSLPTAVFVYTLVNTG
Sbjct: 181 LDGQHSTYHALFPRAWTVYDGEPDPELKVSCRQISPFIPHNYRDSSLPTAVFVYTLVNTG 240
Query: 241 KERAKVSLVFSWANSIGGISHLSGDHVNEPFIGEDGVSGVLLHHKTAKGNPPVTFAIAAC 300
+ERAKVSL+F+WANSIGG SHLSG+HVNEPFI EDGVSGVLLHHKTAKGNPPVTFAIAAC
Sbjct: 241 RERAKVSLLFTWANSIGGNSHLSGNHVNEPFIDEDGVSGVLLHHKTAKGNPPVTFAIAAC 300
Query: 301 ETQNVSVTVLPSFGLSEGSGVTAKDMWDKMVQDGQFDRGNFSSGPSVPSSPGETLCAAVA 360
ETQNVSVTVLPSFGLSEGS +TAKDMWDKMVQDGQFDR NFSSGPS+PSSPGETLCAAVA
Sbjct: 301 ETQNVSVTVLPSFGLSEGSCITAKDMWDKMVQDGQFDRDNFSSGPSMPSSPGETLCAAVA 360
Query: 361 ASAWVEPHGKCTVAFSLSWSSPKVKFLKGFSYHRRYTKFYGTSEKAAQTLAHDALTNYKR 420
ASAWVEPHGKCTVAFSLSWSSPKVKFLKGFSYHRRYTKFYGTS KAAQTLAHDALTNYKR
Sbjct: 361 ASAWVEPHGKCTVAFSLSWSSPKVKFLKGFSYHRRYTKFYGTSGKAAQTLAHDALTNYKR 420
Query: 421 WEEEIEKWQRPILMDERLPEWYKFTLFNELYFLVAGGTVWIDSSVVGKKMANDHDELVAR 480
WEEEIEKWQRP+LMDERLPEWYKFTLFNELYFLVAGGTVWIDSS VGKK + D D L AR
Sbjct: 421 WEEEIEKWQRPVLMDERLPEWYKFTLFNELYFLVAGGTVWIDSSFVGKKASYDQDHL-AR 480
Query: 481 IKEDDIKATEAKVSGRGEEVSRTTTTTTLNDFSGVEFDDEENSTSSSHVSEDELMVPLKS 540
+K DD+KA EAKVSGRGEEVSRTTTTTTL+ F +E+DD ENSTSSSH SEDELMVPLK
Sbjct: 481 LKNDDVKAVEAKVSGRGEEVSRTTTTTTLDGFPSIEYDD-ENSTSSSHASEDELMVPLKR 540
Query: 541 GYTDRSYQTYKVMDPGNTEEDVGRFLYLEGVEYVMWCTYDVHFYASFALLELFPKIELNI 600
GYTDRSYQTYKV++PGNTEEDVGRFLYLEGVEYVMWCTYDVHFYAS+ALLELFPKIELNI
Sbjct: 541 GYTDRSYQTYKVLEPGNTEEDVGRFLYLEGVEYVMWCTYDVHFYASYALLELFPKIELNI 600
Query: 601 QRDFAKAVLSEDGRKVKFLADGKYGIRKVRGAVPHDLGTHDPWNEMNAYNIHDTSRWKDL 660
QRDFAKAVLSEDGRKV+FLA+GKYGIRKVRGAVPHDLGTHDPWNEMNAYNIHDTSRWKDL
Sbjct: 601 QRDFAKAVLSEDGRKVRFLAEGKYGIRKVRGAVPHDLGTHDPWNEMNAYNIHDTSRWKDL 660
Query: 661 NTKFVLQVYRDFAATRDMSFGVDVWPSVRAAIEYMEQFDRDGDGVIENDGFPDQTYDTWT 720
NTKFVLQVYRDFAATRDMSFGVDVWPSVRAAIEYMEQFDRDGDGVIENDGFPDQTYDTWT
Sbjct: 661 NTKFVLQVYRDFAATRDMSFGVDVWPSVRAAIEYMEQFDRDGDGVIENDGFPDQTYDTWT 720
Query: 721 VHGISAYCGCLWVAALQAAAAMAHELGDQEFAETCKSKFLKAKPVLEAKLWNGSYFNYDS 780
VHGISAYCGCLWVAALQAAAAMAHELGDQEFAETCKSKFLKA+PVLEA+LWNGSYFNYDS
Sbjct: 721 VHGISAYCGCLWVAALQAAAAMAHELGDQEFAETCKSKFLKARPVLEAELWNGSYFNYDS 780
Query: 781 GSSSNSKSIQADQLAGQWYTASSGLPPLFDDFKIKSALKKIYDFNVMKIRGGRMGAVNGM 840
GSSSNSKSIQADQLAGQWYTASSGLPPLFDDFKIKSAL+KIYDFNVMK+RGGRMGAVNGM
Sbjct: 781 GSSSNSKSIQADQLAGQWYTASSGLPPLFDDFKIKSALRKIYDFNVMKVRGGRMGAVNGM 840
Query: 841 HPNGKVDETCMQSREIWTGVTYGVAATMILAGMEEEAFKTAEGIFLAGWSEEGFGYWFQT 900
HPNGK+DETCMQSREIWTGVTYGVAATMILAGMEEEAFKTAEGIFLAGWSEEGFGYWFQT
Sbjct: 841 HPNGKIDETCMQSREIWTGVTYGVAATMILAGMEEEAFKTAEGIFLAGWSEEGFGYWFQT 900
Query: 901 PEAWSTDGHYRSLIYMRPLSIWGMQWALSLPKAILEAPKINVMDRIHVSSASTKFFHHET 960
PEAWSTDGHYRSLIYMRPLSIWGMQWALSLPKAIL+APKINVMDRIHVSS++TKFF+HET
Sbjct: 901 PEAWSTDGHYRSLIYMRPLSIWGMQWALSLPKAILDAPKINVMDRIHVSSSNTKFFNHET 960
Query: 961 GVRRIATKAKCFGDSVFNCAC 982
GVRRIATKAKCFGDSVFNCAC
Sbjct: 961 GVRRIATKAKCFGDSVFNCAC 979
BLAST of CmaCh08G010320 vs. ExPASy TrEMBL
Match:
A0A5D3CTQ8 (Non-lysosomal glucosylceramidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold472G00270 PE=3 SV=1)
HSP 1 Score: 1930.6 bits (5000), Expect = 0.0e+00
Identity = 927/981 (94.50%), Postives = 961/981 (97.96%), Query Frame = 0
Query: 1 MVSGNLFHCRKNSWPPDEYISKSTLQLFDFDSASPPEQAWRRKLNGHANLLKEFSVTFVE 60
MVSGNLFHCRKNSWPP+EYISKSTLQLFDFDSASPPEQAWRRKLNGHANLLKEFSVTFVE
Sbjct: 1 MVSGNLFHCRKNSWPPEEYISKSTLQLFDFDSASPPEQAWRRKLNGHANLLKEFSVTFVE 60
Query: 61 AIKMVRLGIRLWSYVREEASQGRKAPIDPFTKEACKPSASQGVPLGGMGSGSISRGFRGE 120
AIKMVRLGIRLWSYVREEASQGRKAPIDPFT+E+CKPSASQGVPLGGMGSGSISRGFRGE
Sbjct: 61 AIKMVRLGIRLWSYVREEASQGRKAPIDPFTRESCKPSASQGVPLGGMGSGSISRGFRGE 120
Query: 121 FRQWQIIPGTCEASPVMANQFSIFISRDGGNKKYASVLAPGQHEGIGKAGDSGISSWGWN 180
FRQWQIIPGTCEASP+MANQFSIF+SRDGG+KKYASVLAPGQHEG+GKAGDSGISSWGWN
Sbjct: 121 FRQWQIIPGTCEASPIMANQFSIFVSRDGGSKKYASVLAPGQHEGLGKAGDSGISSWGWN 180
Query: 181 LDGQHSTYHALFPRAWTVYDGEPDPELKVSCRQISPFIPHNYRDSSLPTAVFVYTLVNTG 240
LDGQHSTYHALFPRAWTVYDGEPDPELKVSCRQISPFIPHNYRDSSLPTAVFVYTLVNTG
Sbjct: 181 LDGQHSTYHALFPRAWTVYDGEPDPELKVSCRQISPFIPHNYRDSSLPTAVFVYTLVNTG 240
Query: 241 KERAKVSLVFSWANSIGGISHLSGDHVNEPFIGEDGVSGVLLHHKTAKGNPPVTFAIAAC 300
+ERAKVSL+F+WANSIGGISHLSG+HVNEPFI EDGVSGVLLHHKTAKGNPPVTFAIAAC
Sbjct: 241 RERAKVSLLFTWANSIGGISHLSGNHVNEPFIDEDGVSGVLLHHKTAKGNPPVTFAIAAC 300
Query: 301 ETQNVSVTVLPSFGLSEGSGVTAKDMWDKMVQDGQFDRGNFSSGPSVPSSPGETLCAAVA 360
ETQNVSVTVLPSFGLSEGS VTAKDMWDKMVQDGQFDR NFSSGPS+PSSPGETLCAAVA
Sbjct: 301 ETQNVSVTVLPSFGLSEGSCVTAKDMWDKMVQDGQFDRDNFSSGPSMPSSPGETLCAAVA 360
Query: 361 ASAWVEPHGKCTVAFSLSWSSPKVKFLKGFSYHRRYTKFYGTSEKAAQTLAHDALTNYKR 420
ASAWVEPHGKCTVAFSLSWSSPKVKFLKGFSYHRRYTKFYGTS KAAQTLAHDALTNYKR
Sbjct: 361 ASAWVEPHGKCTVAFSLSWSSPKVKFLKGFSYHRRYTKFYGTSGKAAQTLAHDALTNYKR 420
Query: 421 WEEEIEKWQRPILMDERLPEWYKFTLFNELYFLVAGGTVWIDSSVVGKKMANDHDELVAR 480
WEEEIEKWQ P+LMDERLPEWYKFTLFNELYFLVAGGTVWIDSS VGKK D D+L AR
Sbjct: 421 WEEEIEKWQMPVLMDERLPEWYKFTLFNELYFLVAGGTVWIDSSFVGKKAPYDQDQL-AR 480
Query: 481 IKEDDIKATEAKVSGRGEEVSRTTTTTTLNDFSGVEFDDEENSTSSSHVSEDELMVPLKS 540
+K D++KA EA VSGRGEEVSRT TT TL++F G+E+DD ENSTSSSH SEDELMVPLK
Sbjct: 481 MKNDEVKAVEATVSGRGEEVSRTITTATLDEFPGIEYDD-ENSTSSSHASEDELMVPLKR 540
Query: 541 GYTDRSYQTYKVMDPGNTEEDVGRFLYLEGVEYVMWCTYDVHFYASFALLELFPKIELNI 600
GYTDRSYQTYKV++PGNTEEDVGRFLYLEGVEYVMWCTYDVHFYAS+ALLELFPKIELNI
Sbjct: 541 GYTDRSYQTYKVLEPGNTEEDVGRFLYLEGVEYVMWCTYDVHFYASYALLELFPKIELNI 600
Query: 601 QRDFAKAVLSEDGRKVKFLADGKYGIRKVRGAVPHDLGTHDPWNEMNAYNIHDTSRWKDL 660
QRDFAKAVLSEDGRKVKFLADGK+GIRKVRGAVPHDLGTHDPWNEMNAYNIHDTSRWKDL
Sbjct: 601 QRDFAKAVLSEDGRKVKFLADGKFGIRKVRGAVPHDLGTHDPWNEMNAYNIHDTSRWKDL 660
Query: 661 NTKFVLQVYRDFAATRDMSFGVDVWPSVRAAIEYMEQFDRDGDGVIENDGFPDQTYDTWT 720
NTKFVLQVYRDFAATRDMSFGVDVWPSVRAAIEYMEQFDRDGDGVIENDGFPDQTYDTWT
Sbjct: 661 NTKFVLQVYRDFAATRDMSFGVDVWPSVRAAIEYMEQFDRDGDGVIENDGFPDQTYDTWT 720
Query: 721 VHGISAYCGCLWVAALQAAAAMAHELGDQEFAETCKSKFLKAKPVLEAKLWNGSYFNYDS 780
VHGISAYCGCLWVAALQAAAAMAHELGD+EFAETCKSKFLKA+PVLEA+LWNGSYFNYDS
Sbjct: 721 VHGISAYCGCLWVAALQAAAAMAHELGDREFAETCKSKFLKARPVLEAELWNGSYFNYDS 780
Query: 781 GSSSNSKSIQADQLAGQWYTASSGLPPLFDDFKIKSALKKIYDFNVMKIRGGRMGAVNGM 840
GSSSNSKSIQADQLAGQWYTASSGLPPLFDDFKIKSAL+KIYDFNVMK+RGGRMGAVNGM
Sbjct: 781 GSSSNSKSIQADQLAGQWYTASSGLPPLFDDFKIKSALRKIYDFNVMKVRGGRMGAVNGM 840
Query: 841 HPNGKVDETCMQSREIWTGVTYGVAATMILAGMEEEAFKTAEGIFLAGWSEEGFGYWFQT 900
HPNGKVDETCMQSREIWTGVTYGVAATMILAGMEEEAFKTAEGIFLAGWSEEGFGYWFQT
Sbjct: 841 HPNGKVDETCMQSREIWTGVTYGVAATMILAGMEEEAFKTAEGIFLAGWSEEGFGYWFQT 900
Query: 901 PEAWSTDGHYRSLIYMRPLSIWGMQWALSLPKAILEAPKINVMDRIHVSSASTKFFHHET 960
PEAWSTDGHYRSLIYMRPLSIWGMQWALSLPKAIL+APKINVMDRIHVSS++TKFF+HET
Sbjct: 901 PEAWSTDGHYRSLIYMRPLSIWGMQWALSLPKAILDAPKINVMDRIHVSSSNTKFFNHET 960
Query: 961 GVRRIATKAKCFGDSVFNCAC 982
GVRRIATKAKCFGDSVFNCAC
Sbjct: 961 GVRRIATKAKCFGDSVFNCAC 979
BLAST of CmaCh08G010320 vs. ExPASy TrEMBL
Match:
A0A1S3C4N3 (Non-lysosomal glucosylceramidase OS=Cucumis melo OX=3656 GN=LOC103496976 PE=3 SV=1)
HSP 1 Score: 1930.6 bits (5000), Expect = 0.0e+00
Identity = 927/981 (94.50%), Postives = 961/981 (97.96%), Query Frame = 0
Query: 1 MVSGNLFHCRKNSWPPDEYISKSTLQLFDFDSASPPEQAWRRKLNGHANLLKEFSVTFVE 60
MVSGNLFHCRKNSWPP+EYISKSTLQLFDFDSASPPEQAWRRKLNGHANLLKEFSVTFVE
Sbjct: 1 MVSGNLFHCRKNSWPPEEYISKSTLQLFDFDSASPPEQAWRRKLNGHANLLKEFSVTFVE 60
Query: 61 AIKMVRLGIRLWSYVREEASQGRKAPIDPFTKEACKPSASQGVPLGGMGSGSISRGFRGE 120
AIKMVRLGIRLWSYVREEASQGRKAPIDPFT+E+CKPSASQGVPLGGMGSGSISRGFRGE
Sbjct: 61 AIKMVRLGIRLWSYVREEASQGRKAPIDPFTRESCKPSASQGVPLGGMGSGSISRGFRGE 120
Query: 121 FRQWQIIPGTCEASPVMANQFSIFISRDGGNKKYASVLAPGQHEGIGKAGDSGISSWGWN 180
FRQWQIIPGTCEASP+MANQFSIF+SRDGG+KKYASVLAPGQHEG+GKAGDSGISSWGWN
Sbjct: 121 FRQWQIIPGTCEASPIMANQFSIFVSRDGGSKKYASVLAPGQHEGLGKAGDSGISSWGWN 180
Query: 181 LDGQHSTYHALFPRAWTVYDGEPDPELKVSCRQISPFIPHNYRDSSLPTAVFVYTLVNTG 240
LDGQHSTYHALFPRAWTVYDGEPDPELKVSCRQISPFIPHNYRDSSLPTAVFVYTLVNTG
Sbjct: 181 LDGQHSTYHALFPRAWTVYDGEPDPELKVSCRQISPFIPHNYRDSSLPTAVFVYTLVNTG 240
Query: 241 KERAKVSLVFSWANSIGGISHLSGDHVNEPFIGEDGVSGVLLHHKTAKGNPPVTFAIAAC 300
+ERAKVSL+F+WANSIGGISHLSG+HVNEPFI EDGVSGVLLHHKTAKGNPPVTFAIAAC
Sbjct: 241 RERAKVSLLFTWANSIGGISHLSGNHVNEPFIDEDGVSGVLLHHKTAKGNPPVTFAIAAC 300
Query: 301 ETQNVSVTVLPSFGLSEGSGVTAKDMWDKMVQDGQFDRGNFSSGPSVPSSPGETLCAAVA 360
ETQNVSVTVLPSFGLSEGS VTAKDMWDKMVQDGQFDR NFSSGPS+PSSPGETLCAAVA
Sbjct: 301 ETQNVSVTVLPSFGLSEGSCVTAKDMWDKMVQDGQFDRDNFSSGPSMPSSPGETLCAAVA 360
Query: 361 ASAWVEPHGKCTVAFSLSWSSPKVKFLKGFSYHRRYTKFYGTSEKAAQTLAHDALTNYKR 420
ASAWVEPHGKCTVAFSLSWSSPKVKFLKGFSYHRRYTKFYGTS KAAQTLAHDALTNYKR
Sbjct: 361 ASAWVEPHGKCTVAFSLSWSSPKVKFLKGFSYHRRYTKFYGTSGKAAQTLAHDALTNYKR 420
Query: 421 WEEEIEKWQRPILMDERLPEWYKFTLFNELYFLVAGGTVWIDSSVVGKKMANDHDELVAR 480
WEEEIEKWQ P+LMDERLPEWYKFTLFNELYFLVAGGTVWIDSS VGKK D D+L AR
Sbjct: 421 WEEEIEKWQMPVLMDERLPEWYKFTLFNELYFLVAGGTVWIDSSFVGKKAPYDQDQL-AR 480
Query: 481 IKEDDIKATEAKVSGRGEEVSRTTTTTTLNDFSGVEFDDEENSTSSSHVSEDELMVPLKS 540
+K D++KA EA VSGRGEEVSRT TT TL++F G+E+DD ENSTSSSH SEDELMVPLK
Sbjct: 481 MKNDEVKAVEATVSGRGEEVSRTITTATLDEFPGIEYDD-ENSTSSSHASEDELMVPLKR 540
Query: 541 GYTDRSYQTYKVMDPGNTEEDVGRFLYLEGVEYVMWCTYDVHFYASFALLELFPKIELNI 600
GYTDRSYQTYKV++PGNTEEDVGRFLYLEGVEYVMWCTYDVHFYAS+ALLELFPKIELNI
Sbjct: 541 GYTDRSYQTYKVLEPGNTEEDVGRFLYLEGVEYVMWCTYDVHFYASYALLELFPKIELNI 600
Query: 601 QRDFAKAVLSEDGRKVKFLADGKYGIRKVRGAVPHDLGTHDPWNEMNAYNIHDTSRWKDL 660
QRDFAKAVLSEDGRKVKFLADGK+GIRKVRGAVPHDLGTHDPWNEMNAYNIHDTSRWKDL
Sbjct: 601 QRDFAKAVLSEDGRKVKFLADGKFGIRKVRGAVPHDLGTHDPWNEMNAYNIHDTSRWKDL 660
Query: 661 NTKFVLQVYRDFAATRDMSFGVDVWPSVRAAIEYMEQFDRDGDGVIENDGFPDQTYDTWT 720
NTKFVLQVYRDFAATRDMSFGVDVWPSVRAAIEYMEQFDRDGDGVIENDGFPDQTYDTWT
Sbjct: 661 NTKFVLQVYRDFAATRDMSFGVDVWPSVRAAIEYMEQFDRDGDGVIENDGFPDQTYDTWT 720
Query: 721 VHGISAYCGCLWVAALQAAAAMAHELGDQEFAETCKSKFLKAKPVLEAKLWNGSYFNYDS 780
VHGISAYCGCLWVAALQAAAAMAHELGD+EFAETCKSKFLKA+PVLEA+LWNGSYFNYDS
Sbjct: 721 VHGISAYCGCLWVAALQAAAAMAHELGDREFAETCKSKFLKARPVLEAELWNGSYFNYDS 780
Query: 781 GSSSNSKSIQADQLAGQWYTASSGLPPLFDDFKIKSALKKIYDFNVMKIRGGRMGAVNGM 840
GSSSNSKSIQADQLAGQWYTASSGLPPLFDDFKIKSAL+KIYDFNVMK+RGGRMGAVNGM
Sbjct: 781 GSSSNSKSIQADQLAGQWYTASSGLPPLFDDFKIKSALRKIYDFNVMKVRGGRMGAVNGM 840
Query: 841 HPNGKVDETCMQSREIWTGVTYGVAATMILAGMEEEAFKTAEGIFLAGWSEEGFGYWFQT 900
HPNGKVDETCMQSREIWTGVTYGVAATMILAGMEEEAFKTAEGIFLAGWSEEGFGYWFQT
Sbjct: 841 HPNGKVDETCMQSREIWTGVTYGVAATMILAGMEEEAFKTAEGIFLAGWSEEGFGYWFQT 900
Query: 901 PEAWSTDGHYRSLIYMRPLSIWGMQWALSLPKAILEAPKINVMDRIHVSSASTKFFHHET 960
PEAWSTDGHYRSLIYMRPLSIWGMQWALSLPKAIL+APKINVMDRIHVSS++TKFF+HET
Sbjct: 901 PEAWSTDGHYRSLIYMRPLSIWGMQWALSLPKAILDAPKINVMDRIHVSSSNTKFFNHET 960
Query: 961 GVRRIATKAKCFGDSVFNCAC 982
GVRRIATKAKCFGDSVFNCAC
Sbjct: 961 GVRRIATKAKCFGDSVFNCAC 979
BLAST of CmaCh08G010320 vs. NCBI nr
Match:
XP_023000039.1 (non-lysosomal glucosylceramidase [Cucurbita maxima])
HSP 1 Score: 2026.1 bits (5248), Expect = 0.0e+00
Identity = 981/981 (100.00%), Postives = 981/981 (100.00%), Query Frame = 0
Query: 1 MVSGNLFHCRKNSWPPDEYISKSTLQLFDFDSASPPEQAWRRKLNGHANLLKEFSVTFVE 60
MVSGNLFHCRKNSWPPDEYISKSTLQLFDFDSASPPEQAWRRKLNGHANLLKEFSVTFVE
Sbjct: 1 MVSGNLFHCRKNSWPPDEYISKSTLQLFDFDSASPPEQAWRRKLNGHANLLKEFSVTFVE 60
Query: 61 AIKMVRLGIRLWSYVREEASQGRKAPIDPFTKEACKPSASQGVPLGGMGSGSISRGFRGE 120
AIKMVRLGIRLWSYVREEASQGRKAPIDPFTKEACKPSASQGVPLGGMGSGSISRGFRGE
Sbjct: 61 AIKMVRLGIRLWSYVREEASQGRKAPIDPFTKEACKPSASQGVPLGGMGSGSISRGFRGE 120
Query: 121 FRQWQIIPGTCEASPVMANQFSIFISRDGGNKKYASVLAPGQHEGIGKAGDSGISSWGWN 180
FRQWQIIPGTCEASPVMANQFSIFISRDGGNKKYASVLAPGQHEGIGKAGDSGISSWGWN
Sbjct: 121 FRQWQIIPGTCEASPVMANQFSIFISRDGGNKKYASVLAPGQHEGIGKAGDSGISSWGWN 180
Query: 181 LDGQHSTYHALFPRAWTVYDGEPDPELKVSCRQISPFIPHNYRDSSLPTAVFVYTLVNTG 240
LDGQHSTYHALFPRAWTVYDGEPDPELKVSCRQISPFIPHNYRDSSLPTAVFVYTLVNTG
Sbjct: 181 LDGQHSTYHALFPRAWTVYDGEPDPELKVSCRQISPFIPHNYRDSSLPTAVFVYTLVNTG 240
Query: 241 KERAKVSLVFSWANSIGGISHLSGDHVNEPFIGEDGVSGVLLHHKTAKGNPPVTFAIAAC 300
KERAKVSLVFSWANSIGGISHLSGDHVNEPFIGEDGVSGVLLHHKTAKGNPPVTFAIAAC
Sbjct: 241 KERAKVSLVFSWANSIGGISHLSGDHVNEPFIGEDGVSGVLLHHKTAKGNPPVTFAIAAC 300
Query: 301 ETQNVSVTVLPSFGLSEGSGVTAKDMWDKMVQDGQFDRGNFSSGPSVPSSPGETLCAAVA 360
ETQNVSVTVLPSFGLSEGSGVTAKDMWDKMVQDGQFDRGNFSSGPSVPSSPGETLCAAVA
Sbjct: 301 ETQNVSVTVLPSFGLSEGSGVTAKDMWDKMVQDGQFDRGNFSSGPSVPSSPGETLCAAVA 360
Query: 361 ASAWVEPHGKCTVAFSLSWSSPKVKFLKGFSYHRRYTKFYGTSEKAAQTLAHDALTNYKR 420
ASAWVEPHGKCTVAFSLSWSSPKVKFLKGFSYHRRYTKFYGTSEKAAQTLAHDALTNYKR
Sbjct: 361 ASAWVEPHGKCTVAFSLSWSSPKVKFLKGFSYHRRYTKFYGTSEKAAQTLAHDALTNYKR 420
Query: 421 WEEEIEKWQRPILMDERLPEWYKFTLFNELYFLVAGGTVWIDSSVVGKKMANDHDELVAR 480
WEEEIEKWQRPILMDERLPEWYKFTLFNELYFLVAGGTVWIDSSVVGKKMANDHDELVAR
Sbjct: 421 WEEEIEKWQRPILMDERLPEWYKFTLFNELYFLVAGGTVWIDSSVVGKKMANDHDELVAR 480
Query: 481 IKEDDIKATEAKVSGRGEEVSRTTTTTTLNDFSGVEFDDEENSTSSSHVSEDELMVPLKS 540
IKEDDIKATEAKVSGRGEEVSRTTTTTTLNDFSGVEFDDEENSTSSSHVSEDELMVPLKS
Sbjct: 481 IKEDDIKATEAKVSGRGEEVSRTTTTTTLNDFSGVEFDDEENSTSSSHVSEDELMVPLKS 540
Query: 541 GYTDRSYQTYKVMDPGNTEEDVGRFLYLEGVEYVMWCTYDVHFYASFALLELFPKIELNI 600
GYTDRSYQTYKVMDPGNTEEDVGRFLYLEGVEYVMWCTYDVHFYASFALLELFPKIELNI
Sbjct: 541 GYTDRSYQTYKVMDPGNTEEDVGRFLYLEGVEYVMWCTYDVHFYASFALLELFPKIELNI 600
Query: 601 QRDFAKAVLSEDGRKVKFLADGKYGIRKVRGAVPHDLGTHDPWNEMNAYNIHDTSRWKDL 660
QRDFAKAVLSEDGRKVKFLADGKYGIRKVRGAVPHDLGTHDPWNEMNAYNIHDTSRWKDL
Sbjct: 601 QRDFAKAVLSEDGRKVKFLADGKYGIRKVRGAVPHDLGTHDPWNEMNAYNIHDTSRWKDL 660
Query: 661 NTKFVLQVYRDFAATRDMSFGVDVWPSVRAAIEYMEQFDRDGDGVIENDGFPDQTYDTWT 720
NTKFVLQVYRDFAATRDMSFGVDVWPSVRAAIEYMEQFDRDGDGVIENDGFPDQTYDTWT
Sbjct: 661 NTKFVLQVYRDFAATRDMSFGVDVWPSVRAAIEYMEQFDRDGDGVIENDGFPDQTYDTWT 720
Query: 721 VHGISAYCGCLWVAALQAAAAMAHELGDQEFAETCKSKFLKAKPVLEAKLWNGSYFNYDS 780
VHGISAYCGCLWVAALQAAAAMAHELGDQEFAETCKSKFLKAKPVLEAKLWNGSYFNYDS
Sbjct: 721 VHGISAYCGCLWVAALQAAAAMAHELGDQEFAETCKSKFLKAKPVLEAKLWNGSYFNYDS 780
Query: 781 GSSSNSKSIQADQLAGQWYTASSGLPPLFDDFKIKSALKKIYDFNVMKIRGGRMGAVNGM 840
GSSSNSKSIQADQLAGQWYTASSGLPPLFDDFKIKSALKKIYDFNVMKIRGGRMGAVNGM
Sbjct: 781 GSSSNSKSIQADQLAGQWYTASSGLPPLFDDFKIKSALKKIYDFNVMKIRGGRMGAVNGM 840
Query: 841 HPNGKVDETCMQSREIWTGVTYGVAATMILAGMEEEAFKTAEGIFLAGWSEEGFGYWFQT 900
HPNGKVDETCMQSREIWTGVTYGVAATMILAGMEEEAFKTAEGIFLAGWSEEGFGYWFQT
Sbjct: 841 HPNGKVDETCMQSREIWTGVTYGVAATMILAGMEEEAFKTAEGIFLAGWSEEGFGYWFQT 900
Query: 901 PEAWSTDGHYRSLIYMRPLSIWGMQWALSLPKAILEAPKINVMDRIHVSSASTKFFHHET 960
PEAWSTDGHYRSLIYMRPLSIWGMQWALSLPKAILEAPKINVMDRIHVSSASTKFFHHET
Sbjct: 901 PEAWSTDGHYRSLIYMRPLSIWGMQWALSLPKAILEAPKINVMDRIHVSSASTKFFHHET 960
Query: 961 GVRRIATKAKCFGDSVFNCAC 982
GVRRIATKAKCFGDSVFNCAC
Sbjct: 961 GVRRIATKAKCFGDSVFNCAC 981
BLAST of CmaCh08G010320 vs. NCBI nr
Match:
KAG6593789.1 (Non-lysosomal glucosylceramidase, partial [Cucurbita argyrosperma subsp. sororia])
HSP 1 Score: 2018.8 bits (5229), Expect = 0.0e+00
Identity = 976/981 (99.49%), Postives = 980/981 (99.90%), Query Frame = 0
Query: 1 MVSGNLFHCRKNSWPPDEYISKSTLQLFDFDSASPPEQAWRRKLNGHANLLKEFSVTFVE 60
MVSGNLFHCRKNSWPPDEYISKSTLQLFDFDSASPPEQAWRRKLNGHANLLKEFSVTFVE
Sbjct: 1 MVSGNLFHCRKNSWPPDEYISKSTLQLFDFDSASPPEQAWRRKLNGHANLLKEFSVTFVE 60
Query: 61 AIKMVRLGIRLWSYVREEASQGRKAPIDPFTKEACKPSASQGVPLGGMGSGSISRGFRGE 120
AIKMVRLGIRLWSYVREEASQGRKAPIDPFTKEACKPSASQGVPLGGMGSGSISRGFRGE
Sbjct: 61 AIKMVRLGIRLWSYVREEASQGRKAPIDPFTKEACKPSASQGVPLGGMGSGSISRGFRGE 120
Query: 121 FRQWQIIPGTCEASPVMANQFSIFISRDGGNKKYASVLAPGQHEGIGKAGDSGISSWGWN 180
FRQWQIIPGTCEASPVMANQFSIFISRDGGNKKYASVLAPGQHEGIGKAGDSGISSWGWN
Sbjct: 121 FRQWQIIPGTCEASPVMANQFSIFISRDGGNKKYASVLAPGQHEGIGKAGDSGISSWGWN 180
Query: 181 LDGQHSTYHALFPRAWTVYDGEPDPELKVSCRQISPFIPHNYRDSSLPTAVFVYTLVNTG 240
LDGQHSTYHALFPRAWTVYDGEPDPELKVSCRQISPFIPHNYRDSSLPTAVFVYTLVNTG
Sbjct: 181 LDGQHSTYHALFPRAWTVYDGEPDPELKVSCRQISPFIPHNYRDSSLPTAVFVYTLVNTG 240
Query: 241 KERAKVSLVFSWANSIGGISHLSGDHVNEPFIGEDGVSGVLLHHKTAKGNPPVTFAIAAC 300
+ERAKVSLVFSWANSIGGISHLSGDHVNEPFIGEDGVSGVLLHHKTAKGNPPVTFAIAAC
Sbjct: 241 RERAKVSLVFSWANSIGGISHLSGDHVNEPFIGEDGVSGVLLHHKTAKGNPPVTFAIAAC 300
Query: 301 ETQNVSVTVLPSFGLSEGSGVTAKDMWDKMVQDGQFDRGNFSSGPSVPSSPGETLCAAVA 360
ETQNVSVTVLPSFGLSEGSGVTAKDMWDKMV DGQFDRGN+SSGPSVPSSPGETLCAAVA
Sbjct: 301 ETQNVSVTVLPSFGLSEGSGVTAKDMWDKMVLDGQFDRGNYSSGPSVPSSPGETLCAAVA 360
Query: 361 ASAWVEPHGKCTVAFSLSWSSPKVKFLKGFSYHRRYTKFYGTSEKAAQTLAHDALTNYKR 420
ASAWVEPHGKCTVAFSLSWSSPKVKFLKGFSYHRRYTKFYGTSEKAAQTLAHDALTNYKR
Sbjct: 361 ASAWVEPHGKCTVAFSLSWSSPKVKFLKGFSYHRRYTKFYGTSEKAAQTLAHDALTNYKR 420
Query: 421 WEEEIEKWQRPILMDERLPEWYKFTLFNELYFLVAGGTVWIDSSVVGKKMANDHDELVAR 480
WEEEIEKWQRPILMDERLPEWYKFTLFNELYFLVAGGTVWIDSSVVGKKMANDHDELVAR
Sbjct: 421 WEEEIEKWQRPILMDERLPEWYKFTLFNELYFLVAGGTVWIDSSVVGKKMANDHDELVAR 480
Query: 481 IKEDDIKATEAKVSGRGEEVSRTTTTTTLNDFSGVEFDDEENSTSSSHVSEDELMVPLKS 540
IKEDD+KATEAKVSGRGEEVSRTTTTTTLNDFSGVEFDDEENSTSSSHVSEDELMVPLKS
Sbjct: 481 IKEDDMKATEAKVSGRGEEVSRTTTTTTLNDFSGVEFDDEENSTSSSHVSEDELMVPLKS 540
Query: 541 GYTDRSYQTYKVMDPGNTEEDVGRFLYLEGVEYVMWCTYDVHFYASFALLELFPKIELNI 600
GYTDRSYQTYKVMDPGNTEEDVGRFLYLEGVEYVMWCTYDVHFYASFALLELFPKIELNI
Sbjct: 541 GYTDRSYQTYKVMDPGNTEEDVGRFLYLEGVEYVMWCTYDVHFYASFALLELFPKIELNI 600
Query: 601 QRDFAKAVLSEDGRKVKFLADGKYGIRKVRGAVPHDLGTHDPWNEMNAYNIHDTSRWKDL 660
QRDFAKAVLSEDGRKVKFLADGKYGIRKVRGAVPHDLGTHDPWNEMNAYNIHDTSRWKDL
Sbjct: 601 QRDFAKAVLSEDGRKVKFLADGKYGIRKVRGAVPHDLGTHDPWNEMNAYNIHDTSRWKDL 660
Query: 661 NTKFVLQVYRDFAATRDMSFGVDVWPSVRAAIEYMEQFDRDGDGVIENDGFPDQTYDTWT 720
NTKFVLQVYRDFAATRDMSFGVDVWPSVRAAIEYMEQFDRDGDGVIENDGFPDQTYDTWT
Sbjct: 661 NTKFVLQVYRDFAATRDMSFGVDVWPSVRAAIEYMEQFDRDGDGVIENDGFPDQTYDTWT 720
Query: 721 VHGISAYCGCLWVAALQAAAAMAHELGDQEFAETCKSKFLKAKPVLEAKLWNGSYFNYDS 780
VHGISAYCGCLWVAALQAAAAMAHELGDQEFAETCKSKFLKAKPVLEAKLWNGSYFNYDS
Sbjct: 721 VHGISAYCGCLWVAALQAAAAMAHELGDQEFAETCKSKFLKAKPVLEAKLWNGSYFNYDS 780
Query: 781 GSSSNSKSIQADQLAGQWYTASSGLPPLFDDFKIKSALKKIYDFNVMKIRGGRMGAVNGM 840
GSSSNSKSIQADQLAGQWYTASSGLPPLFDDFKIKSALKKIYDFNVMKI+GGRMGAVNGM
Sbjct: 781 GSSSNSKSIQADQLAGQWYTASSGLPPLFDDFKIKSALKKIYDFNVMKIKGGRMGAVNGM 840
Query: 841 HPNGKVDETCMQSREIWTGVTYGVAATMILAGMEEEAFKTAEGIFLAGWSEEGFGYWFQT 900
HPNGKVDETCMQSREIWTGVTYGVAATMILAGMEEEAFKTAEGIFLAGWSEEGFGYWFQT
Sbjct: 841 HPNGKVDETCMQSREIWTGVTYGVAATMILAGMEEEAFKTAEGIFLAGWSEEGFGYWFQT 900
Query: 901 PEAWSTDGHYRSLIYMRPLSIWGMQWALSLPKAILEAPKINVMDRIHVSSASTKFFHHET 960
PEAWSTDGHYRSLIYMRPLSIWGMQWALSLPKAILEAPKINVMDRIHVSSASTKFFHHET
Sbjct: 901 PEAWSTDGHYRSLIYMRPLSIWGMQWALSLPKAILEAPKINVMDRIHVSSASTKFFHHET 960
Query: 961 GVRRIATKAKCFGDSVFNCAC 982
GVRRIATKAKCFGDSVFNCAC
Sbjct: 961 GVRRIATKAKCFGDSVFNCAC 981
BLAST of CmaCh08G010320 vs. NCBI nr
Match:
XP_022964300.1 (non-lysosomal glucosylceramidase [Cucurbita moschata])
HSP 1 Score: 2016.9 bits (5224), Expect = 0.0e+00
Identity = 975/981 (99.39%), Postives = 980/981 (99.90%), Query Frame = 0
Query: 1 MVSGNLFHCRKNSWPPDEYISKSTLQLFDFDSASPPEQAWRRKLNGHANLLKEFSVTFVE 60
MVSGNLFHCRKNSWPPDEYISKSTLQLFDFDSASPPEQAWRRKLNGHANLLKEFSVTFVE
Sbjct: 1 MVSGNLFHCRKNSWPPDEYISKSTLQLFDFDSASPPEQAWRRKLNGHANLLKEFSVTFVE 60
Query: 61 AIKMVRLGIRLWSYVREEASQGRKAPIDPFTKEACKPSASQGVPLGGMGSGSISRGFRGE 120
AIKMVRLGIRLWSYVREEASQGRKAPIDPFTKEACKPSASQGVPLGGMGSGSISRGFRGE
Sbjct: 61 AIKMVRLGIRLWSYVREEASQGRKAPIDPFTKEACKPSASQGVPLGGMGSGSISRGFRGE 120
Query: 121 FRQWQIIPGTCEASPVMANQFSIFISRDGGNKKYASVLAPGQHEGIGKAGDSGISSWGWN 180
FRQWQIIPGTCEASPVMANQFSIFISRDGGNKKYASVLAPGQHEGIGKAGDSGISSWGWN
Sbjct: 121 FRQWQIIPGTCEASPVMANQFSIFISRDGGNKKYASVLAPGQHEGIGKAGDSGISSWGWN 180
Query: 181 LDGQHSTYHALFPRAWTVYDGEPDPELKVSCRQISPFIPHNYRDSSLPTAVFVYTLVNTG 240
LDGQHSTYHALFPRAWTVYDGEPDPELKVSCRQISPFIPHNYRDSSLPTAVFVYTLVNTG
Sbjct: 181 LDGQHSTYHALFPRAWTVYDGEPDPELKVSCRQISPFIPHNYRDSSLPTAVFVYTLVNTG 240
Query: 241 KERAKVSLVFSWANSIGGISHLSGDHVNEPFIGEDGVSGVLLHHKTAKGNPPVTFAIAAC 300
+ERAKVSLVFSWANSIGGISHLSGDHVNEPFIGEDGVSGVLLHHKTAKGNPPVTFAIAAC
Sbjct: 241 RERAKVSLVFSWANSIGGISHLSGDHVNEPFIGEDGVSGVLLHHKTAKGNPPVTFAIAAC 300
Query: 301 ETQNVSVTVLPSFGLSEGSGVTAKDMWDKMVQDGQFDRGNFSSGPSVPSSPGETLCAAVA 360
ETQNVSVTVLPSFGLSEGSGVTAKDMWDKMVQDGQFDRGN+SSGPSVPSSPGETLCAAVA
Sbjct: 301 ETQNVSVTVLPSFGLSEGSGVTAKDMWDKMVQDGQFDRGNYSSGPSVPSSPGETLCAAVA 360
Query: 361 ASAWVEPHGKCTVAFSLSWSSPKVKFLKGFSYHRRYTKFYGTSEKAAQTLAHDALTNYKR 420
ASAWVEPHGKCTVAFSLSWSSPKVKFLKGFSYHRRYTKFYGTSEKAAQTLAHDALTNYKR
Sbjct: 361 ASAWVEPHGKCTVAFSLSWSSPKVKFLKGFSYHRRYTKFYGTSEKAAQTLAHDALTNYKR 420
Query: 421 WEEEIEKWQRPILMDERLPEWYKFTLFNELYFLVAGGTVWIDSSVVGKKMANDHDELVAR 480
WEEEIEKWQ PILMDERLPEWYKFTLFNELYFLVAGGTVWIDSSVVGKKMANDHDELVAR
Sbjct: 421 WEEEIEKWQGPILMDERLPEWYKFTLFNELYFLVAGGTVWIDSSVVGKKMANDHDELVAR 480
Query: 481 IKEDDIKATEAKVSGRGEEVSRTTTTTTLNDFSGVEFDDEENSTSSSHVSEDELMVPLKS 540
IKE+DIKATEA+VSGRGEEVSRTTTTTTLNDFSGVEFDDEENSTSSSHVSEDELMVPLKS
Sbjct: 481 IKEEDIKATEAEVSGRGEEVSRTTTTTTLNDFSGVEFDDEENSTSSSHVSEDELMVPLKS 540
Query: 541 GYTDRSYQTYKVMDPGNTEEDVGRFLYLEGVEYVMWCTYDVHFYASFALLELFPKIELNI 600
GYTDRSYQTYKVMDPGNTEEDVGRFLYLEGVEYVMWCTYDVHFYASFALLELFPKIELNI
Sbjct: 541 GYTDRSYQTYKVMDPGNTEEDVGRFLYLEGVEYVMWCTYDVHFYASFALLELFPKIELNI 600
Query: 601 QRDFAKAVLSEDGRKVKFLADGKYGIRKVRGAVPHDLGTHDPWNEMNAYNIHDTSRWKDL 660
QRDFAKAVLSEDGRKVKFLADGKYGIRKVRGAVPHDLGTHDPWNEMNAYNIHDTSRWKDL
Sbjct: 601 QRDFAKAVLSEDGRKVKFLADGKYGIRKVRGAVPHDLGTHDPWNEMNAYNIHDTSRWKDL 660
Query: 661 NTKFVLQVYRDFAATRDMSFGVDVWPSVRAAIEYMEQFDRDGDGVIENDGFPDQTYDTWT 720
NTKFVLQVYRDFAATRDMSFGVDVWPSVRAAIEYMEQFDRDGDGVIENDGFPDQTYDTWT
Sbjct: 661 NTKFVLQVYRDFAATRDMSFGVDVWPSVRAAIEYMEQFDRDGDGVIENDGFPDQTYDTWT 720
Query: 721 VHGISAYCGCLWVAALQAAAAMAHELGDQEFAETCKSKFLKAKPVLEAKLWNGSYFNYDS 780
VHGISAYCGCLWVAALQAAAAMAHELGDQEFAETCKSKFLKAKPVLEAKLWNGSYFNYDS
Sbjct: 721 VHGISAYCGCLWVAALQAAAAMAHELGDQEFAETCKSKFLKAKPVLEAKLWNGSYFNYDS 780
Query: 781 GSSSNSKSIQADQLAGQWYTASSGLPPLFDDFKIKSALKKIYDFNVMKIRGGRMGAVNGM 840
GSSSNSKSIQADQLAGQWYTASSGLPPLFDDFKIKSALKKIYDFNVMKI+GGRMGAVNGM
Sbjct: 781 GSSSNSKSIQADQLAGQWYTASSGLPPLFDDFKIKSALKKIYDFNVMKIKGGRMGAVNGM 840
Query: 841 HPNGKVDETCMQSREIWTGVTYGVAATMILAGMEEEAFKTAEGIFLAGWSEEGFGYWFQT 900
HPNGKVDETCMQSREIWTGVTYGVAATMILAGMEEEAFKTAEGIFLAGWSEEGFGYWFQT
Sbjct: 841 HPNGKVDETCMQSREIWTGVTYGVAATMILAGMEEEAFKTAEGIFLAGWSEEGFGYWFQT 900
Query: 901 PEAWSTDGHYRSLIYMRPLSIWGMQWALSLPKAILEAPKINVMDRIHVSSASTKFFHHET 960
PEAWSTDGHYRSLIYMRPLSIWGMQWALSLPKAILEAPKINVMDRIHVSSASTKFFHHET
Sbjct: 901 PEAWSTDGHYRSLIYMRPLSIWGMQWALSLPKAILEAPKINVMDRIHVSSASTKFFHHET 960
Query: 961 GVRRIATKAKCFGDSVFNCAC 982
GVRRIATKAKCFGDSVFNCAC
Sbjct: 961 GVRRIATKAKCFGDSVFNCAC 981
BLAST of CmaCh08G010320 vs. NCBI nr
Match:
XP_023514140.1 (non-lysosomal glucosylceramidase [Cucurbita pepo subsp. pepo] >XP_023514141.1 non-lysosomal glucosylceramidase [Cucurbita pepo subsp. pepo])
HSP 1 Score: 2015.7 bits (5221), Expect = 0.0e+00
Identity = 974/981 (99.29%), Postives = 980/981 (99.90%), Query Frame = 0
Query: 1 MVSGNLFHCRKNSWPPDEYISKSTLQLFDFDSASPPEQAWRRKLNGHANLLKEFSVTFVE 60
MVSGNLFHCRKNSWPPDEYISKSTLQLFDFDSASPPEQAWRRKLNGHANLLKEFSVTFVE
Sbjct: 1 MVSGNLFHCRKNSWPPDEYISKSTLQLFDFDSASPPEQAWRRKLNGHANLLKEFSVTFVE 60
Query: 61 AIKMVRLGIRLWSYVREEASQGRKAPIDPFTKEACKPSASQGVPLGGMGSGSISRGFRGE 120
AIKMVRLGIRLWSYVREEASQGRKAPIDPFTKEACKPSASQGVPLGGMGSGSISRGFRGE
Sbjct: 61 AIKMVRLGIRLWSYVREEASQGRKAPIDPFTKEACKPSASQGVPLGGMGSGSISRGFRGE 120
Query: 121 FRQWQIIPGTCEASPVMANQFSIFISRDGGNKKYASVLAPGQHEGIGKAGDSGISSWGWN 180
FRQWQIIPGTCEASPVMANQFSIFISRDGGNKKYASVLAPGQHEGIGKAGDSGISSWGWN
Sbjct: 121 FRQWQIIPGTCEASPVMANQFSIFISRDGGNKKYASVLAPGQHEGIGKAGDSGISSWGWN 180
Query: 181 LDGQHSTYHALFPRAWTVYDGEPDPELKVSCRQISPFIPHNYRDSSLPTAVFVYTLVNTG 240
LDGQHSTYHALFPRAWTVYDGEPDPELKVSCRQISPFIPHNYRDSSLPTAVFVYTLVNTG
Sbjct: 181 LDGQHSTYHALFPRAWTVYDGEPDPELKVSCRQISPFIPHNYRDSSLPTAVFVYTLVNTG 240
Query: 241 KERAKVSLVFSWANSIGGISHLSGDHVNEPFIGEDGVSGVLLHHKTAKGNPPVTFAIAAC 300
+ERAKVSLVFSWANSIGGISHLSGDHVNEPFIGEDGVSGVLLHHKTAKGNPPVTFAIAAC
Sbjct: 241 RERAKVSLVFSWANSIGGISHLSGDHVNEPFIGEDGVSGVLLHHKTAKGNPPVTFAIAAC 300
Query: 301 ETQNVSVTVLPSFGLSEGSGVTAKDMWDKMVQDGQFDRGNFSSGPSVPSSPGETLCAAVA 360
ETQNVSVTVLPSFGLSEGSGVTAKDMWDKMVQDGQFDRGNFSSGPSVPSSPGETLCAAVA
Sbjct: 301 ETQNVSVTVLPSFGLSEGSGVTAKDMWDKMVQDGQFDRGNFSSGPSVPSSPGETLCAAVA 360
Query: 361 ASAWVEPHGKCTVAFSLSWSSPKVKFLKGFSYHRRYTKFYGTSEKAAQTLAHDALTNYKR 420
ASAWVEPHGKCTVAFSLSWSSPKVKFLKGFSY RRYTKFYGTSEKAAQTLAHDALTNYKR
Sbjct: 361 ASAWVEPHGKCTVAFSLSWSSPKVKFLKGFSYRRRYTKFYGTSEKAAQTLAHDALTNYKR 420
Query: 421 WEEEIEKWQRPILMDERLPEWYKFTLFNELYFLVAGGTVWIDSSVVGKKMANDHDELVAR 480
WEEEIEKWQRPILMDERLPEWYKFTLFNELYFLVAGGTVWIDSSVVGKKMANDHDELVAR
Sbjct: 421 WEEEIEKWQRPILMDERLPEWYKFTLFNELYFLVAGGTVWIDSSVVGKKMANDHDELVAR 480
Query: 481 IKEDDIKATEAKVSGRGEEVSRTTTTTTLNDFSGVEFDDEENSTSSSHVSEDELMVPLKS 540
IKEDD+KATEAKVSGRGEEVSRTTTTTTLNDFSGVEFD+EENSTSSSHVSEDELMVPLKS
Sbjct: 481 IKEDDMKATEAKVSGRGEEVSRTTTTTTLNDFSGVEFDEEENSTSSSHVSEDELMVPLKS 540
Query: 541 GYTDRSYQTYKVMDPGNTEEDVGRFLYLEGVEYVMWCTYDVHFYASFALLELFPKIELNI 600
GYTDRSYQTYKVMDPGNTEEDVGRFLYLEGVEYVMWCTYDVHFYASFALLELFPKIELNI
Sbjct: 541 GYTDRSYQTYKVMDPGNTEEDVGRFLYLEGVEYVMWCTYDVHFYASFALLELFPKIELNI 600
Query: 601 QRDFAKAVLSEDGRKVKFLADGKYGIRKVRGAVPHDLGTHDPWNEMNAYNIHDTSRWKDL 660
QRDFAKAVLSEDGRKVKFLADGKYGIRKVRGAVPHDLGTHDPWNEMNAYNIHDTSRWKDL
Sbjct: 601 QRDFAKAVLSEDGRKVKFLADGKYGIRKVRGAVPHDLGTHDPWNEMNAYNIHDTSRWKDL 660
Query: 661 NTKFVLQVYRDFAATRDMSFGVDVWPSVRAAIEYMEQFDRDGDGVIENDGFPDQTYDTWT 720
NTKFVLQVYRDFAATRDM+FGVDVWPSVRAAIEYMEQFDRDGDGVIENDGFPDQTYDTWT
Sbjct: 661 NTKFVLQVYRDFAATRDMAFGVDVWPSVRAAIEYMEQFDRDGDGVIENDGFPDQTYDTWT 720
Query: 721 VHGISAYCGCLWVAALQAAAAMAHELGDQEFAETCKSKFLKAKPVLEAKLWNGSYFNYDS 780
VHGISAYCGCLWVAALQAAAAMAHELGDQEFAETCKSKFLKAKPVLEAKLWNGSYFNYDS
Sbjct: 721 VHGISAYCGCLWVAALQAAAAMAHELGDQEFAETCKSKFLKAKPVLEAKLWNGSYFNYDS 780
Query: 781 GSSSNSKSIQADQLAGQWYTASSGLPPLFDDFKIKSALKKIYDFNVMKIRGGRMGAVNGM 840
GSSSNSKSIQADQLAGQWYTASSGLPPLFDDFKIKSALKKIYDFNVMKI+GGRMGAVNGM
Sbjct: 781 GSSSNSKSIQADQLAGQWYTASSGLPPLFDDFKIKSALKKIYDFNVMKIKGGRMGAVNGM 840
Query: 841 HPNGKVDETCMQSREIWTGVTYGVAATMILAGMEEEAFKTAEGIFLAGWSEEGFGYWFQT 900
HPNGKVDETCMQSREIWTGVTYGVAATMILAGMEEEAFKTAEGIFLAGWSE+GFGYWFQT
Sbjct: 841 HPNGKVDETCMQSREIWTGVTYGVAATMILAGMEEEAFKTAEGIFLAGWSEDGFGYWFQT 900
Query: 901 PEAWSTDGHYRSLIYMRPLSIWGMQWALSLPKAILEAPKINVMDRIHVSSASTKFFHHET 960
PEAWSTDGHYRSLIYMRPLSIWGMQWALSLPKAILEAPKINVMDRIHVSSASTKFFHHET
Sbjct: 901 PEAWSTDGHYRSLIYMRPLSIWGMQWALSLPKAILEAPKINVMDRIHVSSASTKFFHHET 960
Query: 961 GVRRIATKAKCFGDSVFNCAC 982
GVRRIATKAKCFGDSVFNCAC
Sbjct: 961 GVRRIATKAKCFGDSVFNCAC 981
BLAST of CmaCh08G010320 vs. NCBI nr
Match:
KAG7026118.1 (Non-lysosomal glucosylceramidase, partial [Cucurbita argyrosperma subsp. argyrosperma])
HSP 1 Score: 2006.1 bits (5196), Expect = 0.0e+00
Identity = 977/1010 (96.73%), Postives = 981/1010 (97.13%), Query Frame = 0
Query: 1 MVSGNLFHCRKNSWPPDEYISKSTLQLFDFDSASPPEQAWRRKLNGHANLLKEFSVTFVE 60
MVSGNLFHCRKNSWPPDEYISKSTLQLFDFDSASPPEQAWRRKLNGHANLLKEFSVTFVE
Sbjct: 1 MVSGNLFHCRKNSWPPDEYISKSTLQLFDFDSASPPEQAWRRKLNGHANLLKEFSVTFVE 60
Query: 61 AIKMVRLGIRLWSYVREEASQGRKAPIDPFTKEACKPSASQGVPLGGMGSGSISRGFRGE 120
AIKMVRLGIRLWSYVREEASQGRKAPIDPFTKEACKPSASQGVPLGGMGSGSISRGFRGE
Sbjct: 61 AIKMVRLGIRLWSYVREEASQGRKAPIDPFTKEACKPSASQGVPLGGMGSGSISRGFRGE 120
Query: 121 FRQWQIIPGTCEASPVMANQFSIFISRDGGNKKYASVLAPGQHEGIGKAGDSGISSWGWN 180
FRQWQIIPGTCEASPVMANQFSIFISRDGGNKKYASVLAPGQHEGIGKAGDSGISSWGWN
Sbjct: 121 FRQWQIIPGTCEASPVMANQFSIFISRDGGNKKYASVLAPGQHEGIGKAGDSGISSWGWN 180
Query: 181 LDGQHSTYHALFPRAWTVYDGEPDPELKVSCRQISPFIPHNYRDSSLPTAVFVYTLVNTG 240
LDGQHSTYHALFPRAWTVYDGEPDPELKVSCRQISPFIPHNYRDSSLPTAVFVYTLVNTG
Sbjct: 181 LDGQHSTYHALFPRAWTVYDGEPDPELKVSCRQISPFIPHNYRDSSLPTAVFVYTLVNTG 240
Query: 241 KERAKVSLVFSWANSIGGISHLSGDHVNEPFIGEDGVSGVLLHHK--------------- 300
+ERAKVSLVFSWANSIGGISHLSGDHVNEPFIGEDGVSGVLLHHK
Sbjct: 241 RERAKVSLVFSWANSIGGISHLSGDHVNEPFIGEDGVSGVLLHHKQVTKPYTLFYACGYG 300
Query: 301 --------------TAKGNPPVTFAIAACETQNVSVTVLPSFGLSEGSGVTAKDMWDKMV 360
TAKGNPPVTFAIAACETQNVSVTVLPSFGLSEGSGVTAKDMWDKMV
Sbjct: 301 RPVKNLKEMCICFRTAKGNPPVTFAIAACETQNVSVTVLPSFGLSEGSGVTAKDMWDKMV 360
Query: 361 QDGQFDRGNFSSGPSVPSSPGETLCAAVAASAWVEPHGKCTVAFSLSWSSPKVKFLKGFS 420
QDGQFDRGN+SSGPSVPSSPGETLCAAVAASAWVEPHGKCTVAFSLSWSSPKVKFLKGFS
Sbjct: 361 QDGQFDRGNYSSGPSVPSSPGETLCAAVAASAWVEPHGKCTVAFSLSWSSPKVKFLKGFS 420
Query: 421 YHRRYTKFYGTSEKAAQTLAHDALTNYKRWEEEIEKWQRPILMDERLPEWYKFTLFNELY 480
YHRRYTKFYGTSEKAAQTLAHDALTNYKRWEEEIEKWQRPILMDERLPEWYKFTLFNELY
Sbjct: 421 YHRRYTKFYGTSEKAAQTLAHDALTNYKRWEEEIEKWQRPILMDERLPEWYKFTLFNELY 480
Query: 481 FLVAGGTVWIDSSVVGKKMANDHDELVARIKEDDIKATEAKVSGRGEEVSRTTTTTTLND 540
FLVAGGTVWIDSSVVGKKMANDHDELVARIKEDD+KATEAKVSGRGEEVSRTTTTTTLND
Sbjct: 481 FLVAGGTVWIDSSVVGKKMANDHDELVARIKEDDMKATEAKVSGRGEEVSRTTTTTTLND 540
Query: 541 FSGVEFDDEENSTSSSHVSEDELMVPLKSGYTDRSYQTYKVMDPGNTEEDVGRFLYLEGV 600
FSGVEFDDEENSTSSSHVSEDELMVPLKSGYTDRSYQTYKVMDPGNTEEDVGRFLYLEGV
Sbjct: 541 FSGVEFDDEENSTSSSHVSEDELMVPLKSGYTDRSYQTYKVMDPGNTEEDVGRFLYLEGV 600
Query: 601 EYVMWCTYDVHFYASFALLELFPKIELNIQRDFAKAVLSEDGRKVKFLADGKYGIRKVRG 660
EYVMWCTYDVHFYASFALLELFPKIELNIQRDFAKAVLSEDGRKVKFLADGKYGIRKVRG
Sbjct: 601 EYVMWCTYDVHFYASFALLELFPKIELNIQRDFAKAVLSEDGRKVKFLADGKYGIRKVRG 660
Query: 661 AVPHDLGTHDPWNEMNAYNIHDTSRWKDLNTKFVLQVYRDFAATRDMSFGVDVWPSVRAA 720
AVPHDLGTHDPWNEMNAYNIHDTSRWKDLNTKFVLQVYRDFAATRDMSFGVDVWPSVRAA
Sbjct: 661 AVPHDLGTHDPWNEMNAYNIHDTSRWKDLNTKFVLQVYRDFAATRDMSFGVDVWPSVRAA 720
Query: 721 IEYMEQFDRDGDGVIENDGFPDQTYDTWTVHGISAYCGCLWVAALQAAAAMAHELGDQEF 780
IEYMEQFDRDGDGVIENDGFPDQTYDTWTVHGISAYCGCLWVAALQAAAAMAHELGDQEF
Sbjct: 721 IEYMEQFDRDGDGVIENDGFPDQTYDTWTVHGISAYCGCLWVAALQAAAAMAHELGDQEF 780
Query: 781 AETCKSKFLKAKPVLEAKLWNGSYFNYDSGSSSNSKSIQADQLAGQWYTASSGLPPLFDD 840
AETCKSKFLKAKPVLEAKLWNGSYFNYDSGSSSNSKSIQADQLAGQWYTASSGLPPLFDD
Sbjct: 781 AETCKSKFLKAKPVLEAKLWNGSYFNYDSGSSSNSKSIQADQLAGQWYTASSGLPPLFDD 840
Query: 841 FKIKSALKKIYDFNVMKIRGGRMGAVNGMHPNGKVDETCMQSREIWTGVTYGVAATMILA 900
FKIKSALKKIYDFNVMKI+GGRMGAVNGMHPNGKVDETCMQSREIWTGVTYGVAATMILA
Sbjct: 841 FKIKSALKKIYDFNVMKIKGGRMGAVNGMHPNGKVDETCMQSREIWTGVTYGVAATMILA 900
Query: 901 GMEEEAFKTAEGIFLAGWSEEGFGYWFQTPEAWSTDGHYRSLIYMRPLSIWGMQWALSLP 960
GMEEEAFKTAEGIFLAGWSEEGFGYWFQTPEAWSTDGHYRSLIYMRPLSIWGMQWALSLP
Sbjct: 901 GMEEEAFKTAEGIFLAGWSEEGFGYWFQTPEAWSTDGHYRSLIYMRPLSIWGMQWALSLP 960
Query: 961 KAILEAPKINVMDRIHVSSASTKFFHHETGVRRIATKAKCFGDSVFNCAC 982
KAILEAPKINVMDRIHVSSASTKFFHHETGVRRIATKAKCFGDSVFNCAC
Sbjct: 961 KAILEAPKINVMDRIHVSSASTKFFHHETGVRRIATKAKCFGDSVFNCAC 1010
BLAST of CmaCh08G010320 vs. TAIR 10
Match:
AT3G24180.1 (Beta-glucosidase, GBA2 type family protein )
HSP 1 Score: 1527.7 bits (3954), Expect = 0.0e+00
Identity = 739/983 (75.18%), Postives = 833/983 (84.74%), Query Frame = 0
Query: 1 MVSGNLFHCRKNSWPPDEYISKSTLQLFDFDSASPPEQAWRRKLNGHANLLKEFSVTFVE 60
MV LFH RK+SWP +E+IS+STLQL DFDSA+PP AWRR+LN HAN+LKEF++TF E
Sbjct: 1 MVGATLFHRRKHSWPTEEFISRSTLQLLDFDSAAPPPHAWRRRLNCHANILKEFTITFRE 60
Query: 61 AIKMVRLGIRLWSYVREEASQGRKAPIDPFTKEACKPSASQGVPLGGMGSGSISRGFRGE 120
AIKMVRLGIRLWSYVREEAS GRKAPIDPFTKE CKPSASQGVPLGGMGSGSISRGFRGE
Sbjct: 61 AIKMVRLGIRLWSYVREEASHGRKAPIDPFTKENCKPSASQGVPLGGMGSGSISRGFRGE 120
Query: 121 FRQWQIIPGTCEASPVMANQFSIFISRDGGNKKYASVLAPGQHEGIGKAGDSGISSWGWN 180
F+QWQI PGTC+ SP+M+NQFSIFISRDGG+KKYASVL+PGQH +GK+ D G+SSWGWN
Sbjct: 121 FKQWQITPGTCDPSPMMSNQFSIFISRDGGHKKYASVLSPGQHGSLGKSRDKGLSSWGWN 180
Query: 181 LDGQHSTYHALFPRAWTVYDGEPDPELKVSCRQISPFIPHNYRDSSLPTAVFVYTLVNTG 240
L+GQHSTYHALFPRAWT+YDGEPDPELK+SCRQISPFIP+NYRDSSLP AVFVYTLVNTG
Sbjct: 181 LNGQHSTYHALFPRAWTIYDGEPDPELKISCRQISPFIPNNYRDSSLPAAVFVYTLVNTG 240
Query: 241 KERAKVSLVFSWANSIGGISHLSGDHVNEPFIGEDGVSGVLLHHKTAKGNPPVTFAIAAC 300
KERAKVSL+F+WANS+GG SH+SG HVNEPFIGEDGVSGVLLHHKT KGNPPVTFAIAA
Sbjct: 241 KERAKVSLLFTWANSMGGTSHMSGGHVNEPFIGEDGVSGVLLHHKTGKGNPPVTFAIAAS 300
Query: 301 ETQNVSVTVLPSFGLSEGSGVTAKDMWDKMVQDGQFDRGNFSSGPSVPSSPGETLCAAVA 360
ETQNV+VTVLP FGLSE S TAKDMWD M QDG+FD+ NF+SGPS PS G+T+CAAV+
Sbjct: 301 ETQNVNVTVLPCFGLSEDSSFTAKDMWDTMEQDGKFDQENFNSGPSTPSLAGDTICAAVS 360
Query: 361 ASAWVEPHGKCTVAFSLSWSSPKVKFLKGFSYHRRYTKFYGTSEKAAQTLAHDALTNYKR 420
ASAWVE HGKCTV+F+LSWSSPKVKF KG +Y RRYTKFYGTS +AA L HDALTNYKR
Sbjct: 361 ASAWVEAHGKCTVSFALSWSSPKVKFSKGSTYDRRYTKFYGTSPRAALDLVHDALTNYKR 420
Query: 421 WEEEIEKWQRPILMDERLPEWYKFTLFNELYFLVAGGTVWIDSSVVGKKMANDHDELVAR 480
WEE+IE WQ PIL DERLPEWYKFTLFNELYFLVAGGTVWIDSS + + H
Sbjct: 421 WEEDIEAWQNPILRDERLPEWYKFTLFNELYFLVAGGTVWIDSSSLNANGNSQH------ 480
Query: 481 IKEDDIKATEAKVSGRGEEVSRTTTTTTLNDFSGVEFDDEENSTSSSHVSEDEL-MVPLK 540
++ + ++ KV G +ND + +D N S S DE+ + +
Sbjct: 481 -QQSGLGNSDGKVGG-----------LDIND----QRNDLGNGNSVGVKSNDEVSAIHNR 540
Query: 541 SG-YTDRSYQTYKVMDPGNTEEDVGRFLYLEGVEYVMWCTYDVHFYASFALLELFPKIEL 600
+G + D + +D G +DVGRFLYLEGVEYVMWCTYDVHFYAS+ALL LFPKIEL
Sbjct: 541 NGLFVDTPH-----VDDG---DDVGRFLYLEGVEYVMWCTYDVHFYASYALLMLFPKIEL 600
Query: 601 NIQRDFAKAVLSEDGRKVKFLADGKYGIRKVRGAVPHDLGTHDPWNEMNAYNIHDTSRWK 660
NIQRDFAKAVLSEDGRKVKFLA+G GIRKVRGAVPHDLG HDPWNEMNAYNIHDTSRWK
Sbjct: 601 NIQRDFAKAVLSEDGRKVKFLAEGNVGIRKVRGAVPHDLGMHDPWNEMNAYNIHDTSRWK 660
Query: 661 DLNTKFVLQVYRDFAATRDMSFGVDVWPSVRAAIEYMEQFDRDGDGVIENDGFPDQTYDT 720
DLN KFVLQVYRDFAAT D FG+DVWP+VRAA+EYMEQFDRD D +IENDGFPDQTYDT
Sbjct: 661 DLNPKFVLQVYRDFAATGDYQFGIDVWPAVRAAMEYMEQFDRDNDDLIENDGFPDQTYDT 720
Query: 721 WTVHGISAYCGCLWVAALQAAAAMAHELGDQEFAETCKSKFLKAKPVLEAKLWNGSYFNY 780
WTVHG+SAYCGCLW+AALQAAAAMA ++GD+ FAE CK+KFL AK LE KLWNGSYFNY
Sbjct: 721 WTVHGVSAYCGCLWLAALQAAAAMALQIGDKFFAELCKNKFLNAKAALETKLWNGSYFNY 780
Query: 781 DSGSSSNSKSIQADQLAGQWYTASSGLPPLFDDFKIKSALKKIYDFNVMKIRGGRMGAVN 840
DSGSSSNSKSIQ DQLAGQWY ASSGLPPLF++ KI+S ++KI+DFNVMK +GG+MGAVN
Sbjct: 781 DSGSSSNSKSIQTDQLAGQWYAASSGLPPLFEESKIRSTMQKIFDFNVMKTKGGKMGAVN 840
Query: 841 GMHPNGKVDETCMQSREIWTGVTYGVAATMILAGMEEEAFKTAEGIFLAGWSEEGFGYWF 900
GMHP+GKVD+TCMQSREIWTGVTY AATMIL+GMEE+ F TAEGIF AGWSEEGFGYWF
Sbjct: 841 GMHPDGKVDDTCMQSREIWTGVTYAAAATMILSGMEEQGFTTAEGIFTAGWSEEGFGYWF 900
Query: 901 QTPEAWSTDGHYRSLIYMRPLSIWGMQWALSLPKAILEAPKINVMDRIHVSSASTKFFHH 960
QTPE W+ DGHYRSLIYMRPL+IWGMQWALSLPKAIL+AP+IN+MDR+H+S S +F ++
Sbjct: 901 QTPEGWTMDGHYRSLIYMRPLAIWGMQWALSLPKAILDAPQINMMDRVHLSPRSRRFSNN 950
Query: 961 ETGVRRIATKAKCFGDSVFNCAC 982
V+ KAKCFG+S +C+C
Sbjct: 961 FKVVKH---KAKCFGNSALSCSC 950
BLAST of CmaCh08G010320 vs. TAIR 10
Match:
AT3G24180.2 (Beta-glucosidase, GBA2 type family protein )
HSP 1 Score: 1527.7 bits (3954), Expect = 0.0e+00
Identity = 739/983 (75.18%), Postives = 833/983 (84.74%), Query Frame = 0
Query: 1 MVSGNLFHCRKNSWPPDEYISKSTLQLFDFDSASPPEQAWRRKLNGHANLLKEFSVTFVE 60
MV LFH RK+SWP +E+IS+STLQL DFDSA+PP AWRR+LN HAN+LKEF++TF E
Sbjct: 1 MVGATLFHRRKHSWPTEEFISRSTLQLLDFDSAAPPPHAWRRRLNCHANILKEFTITFRE 60
Query: 61 AIKMVRLGIRLWSYVREEASQGRKAPIDPFTKEACKPSASQGVPLGGMGSGSISRGFRGE 120
AIKMVRLGIRLWSYVREEAS GRKAPIDPFTKE CKPSASQGVPLGGMGSGSISRGFRGE
Sbjct: 61 AIKMVRLGIRLWSYVREEASHGRKAPIDPFTKENCKPSASQGVPLGGMGSGSISRGFRGE 120
Query: 121 FRQWQIIPGTCEASPVMANQFSIFISRDGGNKKYASVLAPGQHEGIGKAGDSGISSWGWN 180
F+QWQI PGTC+ SP+M+NQFSIFISRDGG+KKYASVL+PGQH +GK+ D G+SSWGWN
Sbjct: 121 FKQWQITPGTCDPSPMMSNQFSIFISRDGGHKKYASVLSPGQHGSLGKSRDKGLSSWGWN 180
Query: 181 LDGQHSTYHALFPRAWTVYDGEPDPELKVSCRQISPFIPHNYRDSSLPTAVFVYTLVNTG 240
L+GQHSTYHALFPRAWT+YDGEPDPELK+SCRQISPFIP+NYRDSSLP AVFVYTLVNTG
Sbjct: 181 LNGQHSTYHALFPRAWTIYDGEPDPELKISCRQISPFIPNNYRDSSLPAAVFVYTLVNTG 240
Query: 241 KERAKVSLVFSWANSIGGISHLSGDHVNEPFIGEDGVSGVLLHHKTAKGNPPVTFAIAAC 300
KERAKVSL+F+WANS+GG SH+SG HVNEPFIGEDGVSGVLLHHKT KGNPPVTFAIAA
Sbjct: 241 KERAKVSLLFTWANSMGGTSHMSGGHVNEPFIGEDGVSGVLLHHKTGKGNPPVTFAIAAS 300
Query: 301 ETQNVSVTVLPSFGLSEGSGVTAKDMWDKMVQDGQFDRGNFSSGPSVPSSPGETLCAAVA 360
ETQNV+VTVLP FGLSE S TAKDMWD M QDG+FD+ NF+SGPS PS G+T+CAAV+
Sbjct: 301 ETQNVNVTVLPCFGLSEDSSFTAKDMWDTMEQDGKFDQENFNSGPSTPSLAGDTICAAVS 360
Query: 361 ASAWVEPHGKCTVAFSLSWSSPKVKFLKGFSYHRRYTKFYGTSEKAAQTLAHDALTNYKR 420
ASAWVE HGKCTV+F+LSWSSPKVKF KG +Y RRYTKFYGTS +AA L HDALTNYKR
Sbjct: 361 ASAWVEAHGKCTVSFALSWSSPKVKFSKGSTYDRRYTKFYGTSPRAALDLVHDALTNYKR 420
Query: 421 WEEEIEKWQRPILMDERLPEWYKFTLFNELYFLVAGGTVWIDSSVVGKKMANDHDELVAR 480
WEE+IE WQ PIL DERLPEWYKFTLFNELYFLVAGGTVWIDSS + + H
Sbjct: 421 WEEDIEAWQNPILRDERLPEWYKFTLFNELYFLVAGGTVWIDSSSLNANGNSQH------ 480
Query: 481 IKEDDIKATEAKVSGRGEEVSRTTTTTTLNDFSGVEFDDEENSTSSSHVSEDEL-MVPLK 540
++ + ++ KV G +ND + +D N S S DE+ + +
Sbjct: 481 -QQSGLGNSDGKVGG-----------LDIND----QRNDLGNGNSVGVKSNDEVSAIHNR 540
Query: 541 SG-YTDRSYQTYKVMDPGNTEEDVGRFLYLEGVEYVMWCTYDVHFYASFALLELFPKIEL 600
+G + D + +D G +DVGRFLYLEGVEYVMWCTYDVHFYAS+ALL LFPKIEL
Sbjct: 541 NGLFVDTPH-----VDDG---DDVGRFLYLEGVEYVMWCTYDVHFYASYALLMLFPKIEL 600
Query: 601 NIQRDFAKAVLSEDGRKVKFLADGKYGIRKVRGAVPHDLGTHDPWNEMNAYNIHDTSRWK 660
NIQRDFAKAVLSEDGRKVKFLA+G GIRKVRGAVPHDLG HDPWNEMNAYNIHDTSRWK
Sbjct: 601 NIQRDFAKAVLSEDGRKVKFLAEGNVGIRKVRGAVPHDLGMHDPWNEMNAYNIHDTSRWK 660
Query: 661 DLNTKFVLQVYRDFAATRDMSFGVDVWPSVRAAIEYMEQFDRDGDGVIENDGFPDQTYDT 720
DLN KFVLQVYRDFAAT D FG+DVWP+VRAA+EYMEQFDRD D +IENDGFPDQTYDT
Sbjct: 661 DLNPKFVLQVYRDFAATGDYQFGIDVWPAVRAAMEYMEQFDRDNDDLIENDGFPDQTYDT 720
Query: 721 WTVHGISAYCGCLWVAALQAAAAMAHELGDQEFAETCKSKFLKAKPVLEAKLWNGSYFNY 780
WTVHG+SAYCGCLW+AALQAAAAMA ++GD+ FAE CK+KFL AK LE KLWNGSYFNY
Sbjct: 721 WTVHGVSAYCGCLWLAALQAAAAMALQIGDKFFAELCKNKFLNAKAALETKLWNGSYFNY 780
Query: 781 DSGSSSNSKSIQADQLAGQWYTASSGLPPLFDDFKIKSALKKIYDFNVMKIRGGRMGAVN 840
DSGSSSNSKSIQ DQLAGQWY ASSGLPPLF++ KI+S ++KI+DFNVMK +GG+MGAVN
Sbjct: 781 DSGSSSNSKSIQTDQLAGQWYAASSGLPPLFEESKIRSTMQKIFDFNVMKTKGGKMGAVN 840
Query: 841 GMHPNGKVDETCMQSREIWTGVTYGVAATMILAGMEEEAFKTAEGIFLAGWSEEGFGYWF 900
GMHP+GKVD+TCMQSREIWTGVTY AATMIL+GMEE+ F TAEGIF AGWSEEGFGYWF
Sbjct: 841 GMHPDGKVDDTCMQSREIWTGVTYAAAATMILSGMEEQGFTTAEGIFTAGWSEEGFGYWF 900
Query: 901 QTPEAWSTDGHYRSLIYMRPLSIWGMQWALSLPKAILEAPKINVMDRIHVSSASTKFFHH 960
QTPE W+ DGHYRSLIYMRPL+IWGMQWALSLPKAIL+AP+IN+MDR+H+S S +F ++
Sbjct: 901 QTPEGWTMDGHYRSLIYMRPLAIWGMQWALSLPKAILDAPQINMMDRVHLSPRSRRFSNN 950
Query: 961 ETGVRRIATKAKCFGDSVFNCAC 982
V+ KAKCFG+S +C+C
Sbjct: 961 FKVVKH---KAKCFGNSALSCSC 950
BLAST of CmaCh08G010320 vs. TAIR 10
Match:
AT5G49900.1 (Beta-glucosidase, GBA2 type family protein )
HSP 1 Score: 951.4 bits (2458), Expect = 5.5e-277
Identity = 484/900 (53.78%), Postives = 616/900 (68.44%), Query Frame = 0
Query: 31 DSASPPEQAWRRKLNGHANLLKEFSVTFVEAIKMVRLGIRLWSYVREEASQGRKAPIDPF 90
D A P W+RK++ +EF+++ E ++ +GIRLW REEA++GR A IDPF
Sbjct: 24 DPAVPASLTWQRKIDSDVKAPREFNLSVKEIFQLAPVGIRLWFLCREEAAKGRLAFIDPF 83
Query: 91 TKEACKPSASQGVPLGGMGSGSISRGFRGEFRQWQIIPGTCEASPVMANQFSIFISRDGG 150
+K + ++S GVPLGG+G+GSI R F+GEF++WQ+ P CE PV+ANQFS F+SR G
Sbjct: 84 SKHSV--TSSHGVPLGGIGAGSIGRSFKGEFQRWQLFPPKCEDEPVLANQFSAFVSRANG 143
Query: 151 NKKYASVLAPGQHEGIGKAGDSGISSWGWNLDGQHSTYHALFPRAWTVYDGEPDPELKVS 210
KKY+SVL P + + +SGI SW WNL G STYHAL+PR+WT+Y+GEPDPEL++
Sbjct: 144 -KKYSSVLCPRNPKLDKQDSESGIGSWDWNLKGDKSTYHALYPRSWTMYEGEPDPELRIV 203
Query: 211 CRQISPFIPHNYRDSSLPTAVFVYTLVNTGKERAKVSLVFSWANSIGGISHLSGDHVNEP 270
CRQ+SPFIPHNY++SS P +VF +TL N G A V+L+F+WANS+GG S SG H N
Sbjct: 204 CRQVSPFIPHNYKESSFPVSVFTFTLHNLGNTTADVTLLFTWANSVGGDSEFSGGHYNSK 263
Query: 271 FIGEDGVSGVLLHHKTAKGNPPVTFAIAACETQNVSVTVLPSFGLS-EGSGVTAKDMWDK 330
DGV GVLLHHKTA G P +++AI+A T VSV+ P F +S + G+TAKDMW
Sbjct: 264 ITMNDGVQGVLLHHKTANGLPSLSYAISAQATDGVSVSACPFFIVSGKQDGITAKDMWQA 323
Query: 331 MVQDGQFDRGNFSSGPSVPSSPGETLCAAVAASAWVEPHGKCTVAFSLSWSSPKVKFLKG 390
+ ++G FD +S S+ S G ++ AAVAAS V P V FSL+W P+V+F G
Sbjct: 324 VKENGSFDHLK-ASEASMQSDHGSSIGAAVAASVTVLPGESRIVTFSLAWDCPEVQFPSG 383
Query: 391 FSYHRRYTKFYGTSEKAAQTLAHDALTNYKRWEEEIEKWQRPILMDERLPEWYKFTLFNE 450
Y RRYTKFYG + AA +AHDA+ + +WE IE WQRPIL D+RLP WY TLFNE
Sbjct: 384 KIYSRRYTKFYGNNGDAAAQIAHDAILGHSQWESWIEDWQRPILEDKRLPAWYPVTLFNE 443
Query: 451 LYFLVAGGTVWIDSSVVGKKMANDHDELVARIKEDDIKATEAKVSGRGEEVSRTTTTTTL 510
LY+L +GGT+W D S +A ++E ++++ +
Sbjct: 444 LYYLNSGGTLWTDGSSPVHSLAG--------VREKKFSLDKSQLGLK------------- 503
Query: 511 NDFSGVEFDDEENSTSSSHVSEDELMVPLKSGYTDRSYQTYKVMDPGNTEEDVGRFLYLE 570
ND +D S S E L + T S K+++ G EE++G FLYLE
Sbjct: 504 NDIDVPHQNDTAVSVLEKMASTLE---ELHASTTSNSAFGTKLLEEG--EENIGHFLYLE 563
Query: 571 GVEYVMWCTYDVHFYASFALLELFPKIELNIQRDFAKAVLSEDGRKVKFLADGKYGIRKV 630
G+EY MW TYDVHFYASFAL+ LFPK+EL+IQRDFA AV+ D KVK L++G++ RKV
Sbjct: 564 GIEYRMWNTYDVHFYASFALVMLFPKLELSIQRDFAAAVMLHDPTKVKTLSEGQWVQRKV 623
Query: 631 RGAVPHDLGTHDPWNEMNAYNIHDTSRWKDLNTKFVLQVYRDFAATRDMSFGVDVWPSVR 690
GAVPHDLG +DPW E+N Y +H+T RWKDLN KFVLQVYRD AT D F VWPSV
Sbjct: 624 LGAVPHDLGINDPWFEVNGYTLHNTDRWKDLNPKFVLQVYRDVVATGDKKFASAVWPSVY 683
Query: 691 AAIEYMEQFDRDGDGVIENDGFPDQTYDTWTVHGISAYCGCLWVAALQAAAAMAHELGDQ 750
A+ YM QFD+DGDG+IEN+GFPDQTYDTW+ G+SAYCG LWVAALQAA+A+A +GD+
Sbjct: 684 VAMAYMAQFDKDGDGMIENEGFPDQTYDTWSASGVSAYCGGLWVAALQAASALARVVGDK 743
Query: 751 EFAETCKSKFLKAKPVLEAKLWNGSYFNYDSGSSSNSKSIQADQLAGQWYTASSGLPPLF 810
+ SKF KAK V E KLWNGSYFNYD+ S S +IQADQLAGQWY +SGL P+
Sbjct: 744 NSQDYFWSKFQKAKVVYEKKLWNGSYFNYDNSGSQYSSTIQADQLAGQWYARASGLLPIV 803
Query: 811 DDFKIKSALKKIYDFNVMKIRGGRMGAVNGMHPNGKVDETCMQSREIWTGVTYGVAATMI 870
D+ K ++AL+K+Y++NVMKI+ G+ GAVNGMHPNGKVD MQSREIW+GVTY ++ATMI
Sbjct: 804 DEDKARTALEKVYNYNVMKIKDGKRGAVNGMHPNGKVDTASMQSREIWSGVTYALSATMI 863
Query: 871 LAGMEEEAFKTAEGIFLAGWSEEGFGYWFQTPEAWSTDGHYRSLIYMRPLSIWGMQWALS 930
G+ E AF+TA GI+ A WSE G GY FQTPE+W+T YRSL YMRPL+IW MQWAL+
Sbjct: 864 QEGLVEMAFQTASGIYEAAWSETGLGYSFQTPESWNTVDEYRSLTYMRPLAIWAMQWALT 893
BLAST of CmaCh08G010320 vs. TAIR 10
Match:
AT1G33700.1 (Beta-glucosidase, GBA2 type family protein )
HSP 1 Score: 898.7 bits (2321), Expect = 4.3e-261
Identity = 464/942 (49.26%), Postives = 621/942 (65.92%), Query Frame = 0
Query: 36 PEQAWRRKLNGHANLLKEFSVTFVEAIKMVRLGIRLWSYVREEASQGRKAPIDPFTKEAC 95
P +W RKLN A EF ++ + + + LG RLW + ++EA++GR + D F K
Sbjct: 18 PPFSWERKLNSQAKTPSEFKLSKRDHMHLFPLGYRLWRHTKDEAAKGRASIFDIFRKHHI 77
Query: 96 KPSASQGVPLGGMGSGSISRGFRGEFRQWQIIPGTCEASPVMANQFSIFISRDGGNKKYA 155
+ GVPLGG+GSGSI R ++GEF+Q+++ P CE +P++ NQFS F+SR GG K++
Sbjct: 78 --TGDHGVPLGGIGSGSIGRSYKGEFQQFKLFPKICEEAPILTNQFSAFVSRPGG-VKHS 137
Query: 156 SVLAPGQHEGI---------GKAGDSGISSWGWNLDGQHSTYHALFPRAWTVYDGEPDPE 215
+VL P + + + G+A + GI SW WN+ G+ STYHAL+PR+WTVYDGEPDPE
Sbjct: 138 TVLCPSKPQVVKDNGGYLCQGQAPNMGIESWDWNMTGEKSTYHALYPRSWTVYDGEPDPE 197
Query: 216 LKVSCRQISPFIPHNYRDSSLPTAVFVYTLVNTGKERAKVSLVFSWANSIGGISHLSGDH 275
L++ RQ+SPFIPHNY +SSLP +VF +T+ NTG E A V+L+F+W NS+GG S L+G H
Sbjct: 198 LRIVSRQVSPFIPHNYEESSLPVSVFNFTVTNTGAEPAIVTLLFTWENSVGGASGLTGQH 257
Query: 276 VNEPFIGEDGVSGVLLHHKTAKGNPPVTFAIAACETQNVSVTVLPSFGLS--EGSGVTAK 335
N +DGV V L HKTA G+PPV++AIAA ET++V V+ P F +S + +TA
Sbjct: 258 FNSTMKAKDGVHAVALQHKTANGHPPVSYAIAAKETEDVRVSSCPCFIVSGTTPNQITAG 317
Query: 336 DMWDKMVQDGQFDRGNFSSGPSVPSSPGETLCAAVAASAWVEPHGKCTVAFSLSWSSPKV 395
DMWD++ ++ FD+ +S PS PG ++ AA+AA V P TV FSLSW P+
Sbjct: 318 DMWDEIKKNASFDK--LTSNACSPSKPGTSIGAAIAAKVKVPPGCDRTVTFSLSWDCPEA 377
Query: 396 KFLKGFSYHRRYTKFYGTSEKAAQTLAHDALTNYKRWEEEIEKWQRPILMDERLPEWYKF 455
+F + +YHRRYT+FYG+ AA +AHDAL N+ WE +IE+WQ P+L D LPEWY+
Sbjct: 378 RFDEK-TYHRRYTRFYGSLGNAAVAMAHDALLNFPEWETQIEEWQAPVLADTTLPEWYRV 437
Query: 456 TLFNELYFLVAGGTVWIDSSVVGKKMANDHDELVARIKEDDIKATEAKVSGRGEEVSRTT 515
TLFNELY+ +GGT+W D L + D I GR ++S
Sbjct: 438 TLFNELYYFNSGGTMWTDG-------------LPPKQSLDSI--------GR-RKISLGL 497
Query: 516 TTTTLNDFSGVEFDDEENSTSSSHVSEDELMVPLKSGYTDRSYQTYKVMDPGNTEEDVGR 575
+T ND D+ N D + + + T + ++ NT E++G+
Sbjct: 498 STIDKND------QDQNNVALDILGRIDAVCSQIHAPLTSNAALGNTMIQ--NTTENIGQ 557
Query: 576 FLYLEGVEYVMWCTYDVHFYASFALLELFPKIELNIQRDFAKAVLSEDGRKVKFLADGKY 635
FLYLEGV+Y+M+ TYDVHFY+SFALL LFPKIEL+IQRDFA AVL D K + ++ G++
Sbjct: 558 FLYLEGVQYLMYNTYDVHFYSSFALLMLFPKIELSIQRDFAAAVLMHDSSKKQVMSSGEF 617
Query: 636 GIRKVRGAVPHDLGTHDPWNEMNAYNIHDTSRWKDLNTKFVLQVYRDFAATRDMSFGVDV 695
RKV GAVPHD+G +DPW E+NAYN+ +T RWKDLN+KFVLQVYRD AT D++F V
Sbjct: 618 VTRKVLGAVPHDIGLNDPWFELNAYNLFNTDRWKDLNSKFVLQVYRDVVATGDLNFAKAV 677
Query: 696 WPSVRAAIEYMEQFDRDGDGVIENDGFPDQTYDTWTVHGISAYCGCLWVAALQAAAAMAH 755
WPSV AI Y++QFD+DGDG+IEN+GFPDQTYD W+ G+SAYCG LWVAALQA +A+A
Sbjct: 678 WPSVYTAIAYLDQFDKDGDGMIENEGFPDQTYDAWSCSGVSAYCGGLWVAALQAGSALAR 737
Query: 756 ELGDQEFAETCKSKFLKAKPVLEAKLWNGSYFNYDSGSSSNSKSIQADQLAGQWYTASSG 815
E+GD A +K+ KA+ V E KLWNGSYFNYD+ S +S SI ADQ+AGQWY + G
Sbjct: 738 EIGDNGAAVYFNAKYEKARSVYE-KLWNGSYFNYDNSRSGSSSSILADQMAGQWYARACG 797
Query: 816 LPPLFDDFKIKSALKKIYDFNVMKIRGGRMGAVNGMHPNGKVDETCMQSREIWTGVTYGV 875
L P+ + IK AL+ +YDFNVM++R G GAVNGM P+G+VD + M SRE+W G TY V
Sbjct: 798 LKPIAKEEWIKKALETVYDFNVMRVRDGTRGAVNGMLPDGRVDTSTMVSREVWAGTTYSV 857
Query: 876 AATMILAGMEEEAFKTAEGIFLAGWSEEGFGYWFQTPEAWSTDGHYRSLIYMRPLSIWGM 935
AA MI G+ ++ F+TA GI+ A WS+ G G FQTPEAW+T+ YRSL YMRPL+IWG+
Sbjct: 858 AACMIQEGLADKGFRTASGIYEAAWSDRGLGCAFQTPEAWTTNDEYRSLCYMRPLAIWGI 917
Query: 936 QWALSLPKAILEAPKINVMDRIHVSSASTKFFHHETGVRRIA 967
QWA ++PK E V R A++ F G ++A
Sbjct: 918 QWAHTMPKPNREQ---EVSLRPQEEDATSVLFQQHAGFIKVA 919
BLAST of CmaCh08G010320 vs. TAIR 10
Match:
AT1G33700.2 (Beta-glucosidase, GBA2 type family protein )
HSP 1 Score: 898.7 bits (2321), Expect = 4.3e-261
Identity = 464/942 (49.26%), Postives = 621/942 (65.92%), Query Frame = 0
Query: 36 PEQAWRRKLNGHANLLKEFSVTFVEAIKMVRLGIRLWSYVREEASQGRKAPIDPFTKEAC 95
P +W RKLN A EF ++ + + + LG RLW + ++EA++GR + D F K
Sbjct: 18 PPFSWERKLNSQAKTPSEFKLSKRDHMHLFPLGYRLWRHTKDEAAKGRASIFDIFRKHHI 77
Query: 96 KPSASQGVPLGGMGSGSISRGFRGEFRQWQIIPGTCEASPVMANQFSIFISRDGGNKKYA 155
+ GVPLGG+GSGSI R ++GEF+Q+++ P CE +P++ NQFS F+SR GG K++
Sbjct: 78 --TGDHGVPLGGIGSGSIGRSYKGEFQQFKLFPKICEEAPILTNQFSAFVSRPGG-VKHS 137
Query: 156 SVLAPGQHEGI---------GKAGDSGISSWGWNLDGQHSTYHALFPRAWTVYDGEPDPE 215
+VL P + + + G+A + GI SW WN+ G+ STYHAL+PR+WTVYDGEPDPE
Sbjct: 138 TVLCPSKPQVVKDNGGYLCQGQAPNMGIESWDWNMTGEKSTYHALYPRSWTVYDGEPDPE 197
Query: 216 LKVSCRQISPFIPHNYRDSSLPTAVFVYTLVNTGKERAKVSLVFSWANSIGGISHLSGDH 275
L++ RQ+SPFIPHNY +SSLP +VF +T+ NTG E A V+L+F+W NS+GG S L+G H
Sbjct: 198 LRIVSRQVSPFIPHNYEESSLPVSVFNFTVTNTGAEPAIVTLLFTWENSVGGASGLTGQH 257
Query: 276 VNEPFIGEDGVSGVLLHHKTAKGNPPVTFAIAACETQNVSVTVLPSFGLS--EGSGVTAK 335
N +DGV V L HKTA G+PPV++AIAA ET++V V+ P F +S + +TA
Sbjct: 258 FNSTMKAKDGVHAVALQHKTANGHPPVSYAIAAKETEDVRVSSCPCFIVSGTTPNQITAG 317
Query: 336 DMWDKMVQDGQFDRGNFSSGPSVPSSPGETLCAAVAASAWVEPHGKCTVAFSLSWSSPKV 395
DMWD++ ++ FD+ +S PS PG ++ AA+AA V P TV FSLSW P+
Sbjct: 318 DMWDEIKKNASFDK--LTSNACSPSKPGTSIGAAIAAKVKVPPGCDRTVTFSLSWDCPEA 377
Query: 396 KFLKGFSYHRRYTKFYGTSEKAAQTLAHDALTNYKRWEEEIEKWQRPILMDERLPEWYKF 455
+F + +YHRRYT+FYG+ AA +AHDAL N+ WE +IE+WQ P+L D LPEWY+
Sbjct: 378 RFDEK-TYHRRYTRFYGSLGNAAVAMAHDALLNFPEWETQIEEWQAPVLADTTLPEWYRV 437
Query: 456 TLFNELYFLVAGGTVWIDSSVVGKKMANDHDELVARIKEDDIKATEAKVSGRGEEVSRTT 515
TLFNELY+ +GGT+W D L + D I GR ++S
Sbjct: 438 TLFNELYYFNSGGTMWTDG-------------LPPKQSLDSI--------GR-RKISLGL 497
Query: 516 TTTTLNDFSGVEFDDEENSTSSSHVSEDELMVPLKSGYTDRSYQTYKVMDPGNTEEDVGR 575
+T ND D+ N D + + + T + ++ NT E++G+
Sbjct: 498 STIDKND------QDQNNVALDILGRIDAVCSQIHAPLTSNAALGNTMIQ--NTTENIGQ 557
Query: 576 FLYLEGVEYVMWCTYDVHFYASFALLELFPKIELNIQRDFAKAVLSEDGRKVKFLADGKY 635
FLYLEGV+Y+M+ TYDVHFY+SFALL LFPKIEL+IQRDFA AVL D K + ++ G++
Sbjct: 558 FLYLEGVQYLMYNTYDVHFYSSFALLMLFPKIELSIQRDFAAAVLMHDSSKKQVMSSGEF 617
Query: 636 GIRKVRGAVPHDLGTHDPWNEMNAYNIHDTSRWKDLNTKFVLQVYRDFAATRDMSFGVDV 695
RKV GAVPHD+G +DPW E+NAYN+ +T RWKDLN+KFVLQVYRD AT D++F V
Sbjct: 618 VTRKVLGAVPHDIGLNDPWFELNAYNLFNTDRWKDLNSKFVLQVYRDVVATGDLNFAKAV 677
Query: 696 WPSVRAAIEYMEQFDRDGDGVIENDGFPDQTYDTWTVHGISAYCGCLWVAALQAAAAMAH 755
WPSV AI Y++QFD+DGDG+IEN+GFPDQTYD W+ G+SAYCG LWVAALQA +A+A
Sbjct: 678 WPSVYTAIAYLDQFDKDGDGMIENEGFPDQTYDAWSCSGVSAYCGGLWVAALQAGSALAR 737
Query: 756 ELGDQEFAETCKSKFLKAKPVLEAKLWNGSYFNYDSGSSSNSKSIQADQLAGQWYTASSG 815
E+GD A +K+ KA+ V E KLWNGSYFNYD+ S +S SI ADQ+AGQWY + G
Sbjct: 738 EIGDNGAAVYFNAKYEKARSVYE-KLWNGSYFNYDNSRSGSSSSILADQMAGQWYARACG 797
Query: 816 LPPLFDDFKIKSALKKIYDFNVMKIRGGRMGAVNGMHPNGKVDETCMQSREIWTGVTYGV 875
L P+ + IK AL+ +YDFNVM++R G GAVNGM P+G+VD + M SRE+W G TY V
Sbjct: 798 LKPIAKEEWIKKALETVYDFNVMRVRDGTRGAVNGMLPDGRVDTSTMVSREVWAGTTYSV 857
Query: 876 AATMILAGMEEEAFKTAEGIFLAGWSEEGFGYWFQTPEAWSTDGHYRSLIYMRPLSIWGM 935
AA MI G+ ++ F+TA GI+ A WS+ G G FQTPEAW+T+ YRSL YMRPL+IWG+
Sbjct: 858 AACMIQEGLADKGFRTASGIYEAAWSDRGLGCAFQTPEAWTTNDEYRSLCYMRPLAIWGI 917
Query: 936 QWALSLPKAILEAPKINVMDRIHVSSASTKFFHHETGVRRIA 967
QWA ++PK E V R A++ F G ++A
Sbjct: 918 QWAHTMPKPNREQ---EVSLRPQEEDATSVLFQQHAGFIKVA 919
The following BLAST results are available for this feature:
Match Name | E-value | Identity | Description | |
Q5M868 | 2.6e-178 | 41.02 | Non-lysosomal glucosylceramidase OS=Rattus norvegicus OX=10116 GN=Gba2 PE=2 SV=2 | [more] |
Q9HCG7 | 4.1e-176 | 40.90 | Non-lysosomal glucosylceramidase OS=Homo sapiens OX=9606 GN=GBA2 PE=1 SV=2 | [more] |
Q69ZF3 | 9.2e-176 | 40.68 | Non-lysosomal glucosylceramidase OS=Mus musculus OX=10090 GN=Gba2 PE=1 SV=2 | [more] |
Q7KT91 | 4.2e-136 | 33.44 | Non-lysosomal glucosylceramidase OS=Drosophila melanogaster OX=7227 GN=CG33090 P... | [more] |
Match Name | E-value | Identity | Description | |
A0A6J1KIS9 | 0.0e+00 | 100.00 | Non-lysosomal glucosylceramidase OS=Cucurbita maxima OX=3661 GN=LOC111494347 PE=... | [more] |
A0A6J1HKE8 | 0.0e+00 | 99.39 | Non-lysosomal glucosylceramidase OS=Cucurbita moschata OX=3662 GN=LOC111464357 P... | [more] |
A0A0A0LK88 | 0.0e+00 | 94.60 | Non-lysosomal glucosylceramidase OS=Cucumis sativus OX=3659 GN=Csa_2G130670 PE=3... | [more] |
A0A5D3CTQ8 | 0.0e+00 | 94.50 | Non-lysosomal glucosylceramidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676... | [more] |
A0A1S3C4N3 | 0.0e+00 | 94.50 | Non-lysosomal glucosylceramidase OS=Cucumis melo OX=3656 GN=LOC103496976 PE=3 SV... | [more] |
Match Name | E-value | Identity | Description | |
XP_023000039.1 | 0.0e+00 | 100.00 | non-lysosomal glucosylceramidase [Cucurbita maxima] | [more] |
KAG6593789.1 | 0.0e+00 | 99.49 | Non-lysosomal glucosylceramidase, partial [Cucurbita argyrosperma subsp. sororia... | [more] |
XP_022964300.1 | 0.0e+00 | 99.39 | non-lysosomal glucosylceramidase [Cucurbita moschata] | [more] |
XP_023514140.1 | 0.0e+00 | 99.29 | non-lysosomal glucosylceramidase [Cucurbita pepo subsp. pepo] >XP_023514141.1 no... | [more] |
KAG7026118.1 | 0.0e+00 | 96.73 | Non-lysosomal glucosylceramidase, partial [Cucurbita argyrosperma subsp. argyros... | [more] |