CmaCh08G010100 (gene) Cucurbita maxima (Rimu) v1.1

Overview


Name	CmaCh08G010100
Type	gene
Organism	Cucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Description	non-specific phospholipase C3-like
Location	Cma_Chr08: 6334828 .. 6335352 (-)
RNA-Seq Expression	CmaCh08G010100
Synteny	CmaCh08G010100

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTCTCCGGCTATCAACGCCACCGCCGCCGGCGATGGTAAATCCACCGCAAGTCCGATAAAGACGATCGTCATTCTAGTACAAGAGAATCGATCCTTCGATCACATGCTCGGCTGGATGAAGTCTCTAAATCCAGAAATCGACGGCGTCACAAATGAAAACCGATTCTCGAACCCTGTCTCCACCTCCGATCCAAATTCGCCTTCTATTTTCTTCGGAAACGCCTCCGCCTACGTCGATCCTGATCCCGGCCACTCAATCCAAGACATATACGAGCAGATTTTCGCCGAGCCGTGGTCCGACGCCTCTCAATCGAAAGATCCTCTTCCGCCAGCGACGATGCGAGGCTTCGCACAGAACGCAGAGCGGATCGAAAAAGGAATGTCGGCAACAGTGATGAACTGATTCAAGCCGGAGGCGGTGCCGGTGTTCAAAGAGTTGGTGATGGAGTTCGGAGTGTGTGACCGGTGGTTCGCGTCGGTGCCGGCTGTTTGTGCACTCGGCGACGTCGTTTGGACTGAGTAG

mRNA sequence

ATGTCTCCGGCTATCAACGCCACCGCCGCCGGCGATGGTAAATCCACCGCAAGTCCGATAAAGACGATCGTCATTCTAGTACAAGAGAATCGATCCTTCGATCACATGCTCGGCTGGATGAAGTCTCTAAATCCAGAAATCGACGGCGTCACAAATGAAAACCGATTCTCGAACCCTGTCTCCACCTCCGATCCAAATTCGCCTTCTATTTTCTTCGGAAACGCCTCCGCCTACGTCGATCCTGATCCCGGCCACTCAATCCAAGACATATACGAGCAGATTTTCGCCGAGCCGTGGTCCGACGCCTCTCAATCGAAAGATCCTCTTCCGCCAGCGACGATGCGAGGCTTCGCACAGAACGCAGAGCGGATCGAAAAAGGAATGTCGGCAACACCGGAGGCGGTGCCGGTGTTCAAAGAGTTGGTGATGGAGTTCGGAGTGTGTGACCGGTGGTTCGCGTCGGTGCCGGCTGTTTGTGCACTCGGCGACGTCGTTTGGACTGAGTAG

Coding sequence (CDS)

ATGTCTCCGGCTATCAACGCCACCGCCGCCGGCGATGGTAAATCCACCGCAAGTCCGATAAAGACGATCGTCATTCTAGTACAAGAGAATCGATCCTTCGATCACATGCTCGGCTGGATGAAGTCTCTAAATCCAGAAATCGACGGCGTCACAAATGAAAACCGATTCTCGAACCCTGTCTCCACCTCCGATCCAAATTCGCCTTCTATTTTCTTCGGAAACGCCTCCGCCTACGTCGATCCTGATCCCGGCCACTCAATCCAAGACATATACGAGCAGATTTTCGCCGAGCCGTGGTCCGACGCCTCTCAATCGAAAGATCCTCTTCCGCCAGCGACGATGCGAGGCTTCGCACAGAACGCAGAGCGGATCGAAAAAGGAATGTCGGCAACACCGGAGGCGGTGCCGGTGTTCAAAGAGTTGGTGATGGAGTTCGGAGTGTGTGACCGGTGGTTCGCGTCGGTGCCGGCTGTTTGTGCACTCGGCGACGTCGTTTGGACTGAGTAG

Protein sequence

MSPAINATAAGDGKSTASPIKTIVILVQENRSFDHMLGWMKSLNPEIDGVTNENRFSNPVSTSDPNSPSIFFGNASAYVDPDPGHSIQDIYEQIFAEPWSDASQSKDPLPPATMRGFAQNAERIEKGMSATPEAVPVFKELVMEFGVCDRWFASVPAVCALGDVVWTE

Homology

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BLAST of CmaCh08G010100 vs. ExPASy Swiss-Prot
Match: Q9SRQ6 (Non-specific phospholipase C3 OS=Arabidopsis thaliana OX=3702 GN=NPC3 PE=1 SV=1)

HSP 1 Score: 194.9 bits (494), Expect = 7.3e-49
Identity = 100/160 (62.50%), Postives = 115/160 (71.88%), Query Frame = 0


Query: 5   INATAAGDGKSTASPIKTIVILVQENRSFDHMLGWMKSLNPEIDGVTNENRFSNPVSTSD 64
           +  T++G G S+ASPIKTIV+LVQENRSFDHMLGW K LNPEIDGV+     SNP+STSD
Sbjct: 2   VEETSSGGG-SSASPIKTIVVLVQENRSFDHMLGWFKELNPEIDGVSESEPRSNPLSTSD 61

Query: 65  PNSPSIFFGNASAYVDPDPGHSIQDIYEQIFAEPWSDASQSKDPLPPATMRGFAQNAERI 124
           PNS  IFFG  S  +DPDPGHS Q IYEQ+F +P+SD S    P P   M GF QNAE I
Sbjct: 62  PNSAQIFFGKESQNIDPDPGHSFQAIYEQVFGKPFSDES----PYPDPKMNGFVQNAEAI 121

Query: 125 EKGMSA-------TPEAVPVFKELVMEFGVCDRWFASVPA 158
            KGMS         PE +PVFKELV EF VCDRWF+S+P+
Sbjct: 122 TKGMSEKVVMQGFPPEKLPVFKELVQEFAVCDRWFSSLPS 156

[Close]

BLAST of CmaCh08G010100 vs. ExPASy Swiss-Prot
Match: Q9SRQ7 (Non-specific phospholipase C4 OS=Arabidopsis thaliana OX=3702 GN=NPC4 PE=1 SV=1)

HSP 1 Score: 183.3 bits (464), Expect = 2.2e-45
Identity = 93/151 (61.59%), Postives = 108/151 (71.52%), Query Frame = 0


Query: 13  GKSTASPIKTIVILVQENRSFDHMLGWMKSLNPEIDGVTNENRFSNPVSTSDPNSPSIFF 72
           G S + PIKTIV+LVQENRSFDH LGW K LN EIDGVT  +  SN VS+SD NS  + F
Sbjct: 7   GGSGSYPIKTIVVLVQENRSFDHTLGWFKELNREIDGVTKSDPKSNTVSSSDTNSLRVVF 66

Query: 73  GNASAYVDPDPGHSIQDIYEQIFAEPWSDASQSKDPLPPATMRGFAQNAERIEKGMSAT- 132
           G+ S YV+PDPGHSIQDIYEQ+F +PW       +P  P  M GFAQNAER +KGMS+  
Sbjct: 67  GDQSQYVNPDPGHSIQDIYEQVFGKPWDSGKPDPNPGHP-NMSGFAQNAERNKKGMSSAV 126

Query: 133 -----PEAVPVFKELVMEFGVCDRWFASVPA 158
                P A+PV+KELV  F +CDRWFASVPA
Sbjct: 127 MNGFKPNALPVYKELVQNFAICDRWFASVPA 156

[Close]

BLAST of CmaCh08G010100 vs. ExPASy Swiss-Prot
Match: Q9S816 (Non-specific phospholipase C5 OS=Arabidopsis thaliana OX=3702 GN=NPC5 PE=1 SV=1)

HSP 1 Score: 178.7 bits (452), Expect = 5.4e-44
Identity = 92/149 (61.74%), Postives = 109/149 (73.15%), Query Frame = 0


Query: 15  STASPIKTIVILVQENRSFDHMLGWMKSLNPEIDGVTNENRFSNPVSTSDPNSPSIFFGN 74
           S + PIKTIV+LVQENRSFDH LGW K LN EIDGV   ++  NP  +SD NS ++ FG+
Sbjct: 8   SESYPIKTIVVLVQENRSFDHTLGWFKELNREIDGVMKSDQKFNPGFSSDLNSHNVVFGD 67

Query: 75  ASAYVDPDPGHSIQDIYEQIFAEPWSDASQSKDPLP-PATMRGFAQNAERIEKGMSAT-- 134
            S YVDP+PGHSI+DIYEQ+F +PW   S   DP P PATM GFAQNAER  KGMS+   
Sbjct: 68  QSQYVDPNPGHSIRDIYEQVFGKPWD--SGHPDPNPGPATMSGFAQNAERKMKGMSSAVM 127

Query: 135 ----PEAVPVFKELVMEFGVCDRWFASVP 157
               P+A+PV+KELV  F +CDRWFASVP
Sbjct: 128 NGFKPDALPVYKELVQNFAICDRWFASVP 154

[Close]

BLAST of CmaCh08G010100 vs. ExPASy Swiss-Prot
Match: O81020 (Non-specific phospholipase C2 OS=Arabidopsis thaliana OX=3702 GN=NPC2 PE=2 SV=1)

HSP 1 Score: 148.3 bits (373), Expect = 7.9e-35
Identity = 85/148 (57.43%), Postives = 97/148 (65.54%), Query Frame = 0


Query: 18  SPIKTIVILVQENRSFDHMLGWMKSLNPEIDGVTNENRFSNPVSTSDPNSPSIFFGNASA 77
           SPIKTIV++V ENRSFDHMLGWMK LNPEI+GV      SNPVS SDP+S  I FG+ S 
Sbjct: 25  SPIKTIVVVVMENRSFDHMLGWMKKLNPEINGVDGSE--SNPVSVSDPSSRKIKFGSGSH 84

Query: 78  YVDPDPGHSIQDIYEQIFAEPWSDASQSKDPLPPATMRGFAQNA--ERIEKGMSAT---- 137
           YVDPDPGHS Q I EQ+F    +D S     + P  M GF Q A  E     MSA+    
Sbjct: 85  YVDPDPGHSFQAIREQVFGS--NDTS-----MDPPPMNGFVQQAYSEDPSGNMSASVMNG 144

Query: 138 --PEAVPVFKELVMEFGVCDRWFASVPA 158
             P+ VPV+K LV EF V DRWFASVP+
Sbjct: 145 FEPDKVPVYKSLVSEFAVFDRWFASVPS 163

[Close]

BLAST of CmaCh08G010100 vs. ExPASy Swiss-Prot
Match: Q8L7Y9 (Non-specific phospholipase C1 OS=Arabidopsis thaliana OX=3702 GN=NPC1 PE=2 SV=1)

HSP 1 Score: 144.1 bits (362), Expect = 1.5e-33
Identity = 77/144 (53.47%), Postives = 93/144 (64.58%), Query Frame = 0


Query: 19  PIKTIVILVQENRSFDHMLGWMKSLNPEIDGVTNENRFSNPVSTSDPNSPSIFFGNASAY 78
           PIKTIV++V ENRSFDH+LGW+KS  PEIDG+T +   SNP++ SDPNS  IF  + + +
Sbjct: 37  PIKTIVVVVMENRSFDHILGWLKSTRPEIDGLTGKE--SNPLNVSDPNSKKIFVSDDAVF 96

Query: 79  VDPDPGHSIQDIYEQIFAEPWSDASQSKDPLPPATMRGFAQNAERIEKGMSAT------P 138
           VD DPGHS Q I EQIF         S D      M GFAQ +E +E GM+        P
Sbjct: 97  VDMDPGHSFQAIREQIFG--------SNDTSGDPKMNGFAQQSESMEPGMAKNVMSGFKP 156

Query: 139 EAVPVFKELVMEFGVCDRWFASVP 157
           E +PV+ EL  EFGV DRWFASVP
Sbjct: 157 EVLPVYTELANEFGVFDRWFASVP 170

[Close]

BLAST of CmaCh08G010100 vs. ExPASy TrEMBL
Match: A0A6J1KM42 (LOW QUALITY PROTEIN: non-specific phospholipase C3-like OS=Cucurbita maxima OX=3661 GN=LOC111494535 PE=4 SV=1)

HSP 1 Score: 312.0 bits (798), Expect = 1.5e-81
Identity = 156/162 (96.30%), Postives = 156/162 (96.30%), Query Frame = 0


Query: 1   MSPAINATAAGDGKSTASPIKTIVILVQENRSFDHMLGWMKSLNPEIDGVTNENRFSNPV 60
           MSPAINATAAGDGKSTASPIKTIVILVQENRSFDHMLGWMKSLNPEIDGVTNENRFSNPV
Sbjct: 1   MSPAINATAAGDGKSTASPIKTIVILVQENRSFDHMLGWMKSLNPEIDGVTNENRFSNPV 60

Query: 61  STSDPNSPSIFFGNASAYVDPDPGHSIQDIYEQIFAEPWSDASQSKDPLPPATMRGFAQN 120
           STSDPNSPSIFFGNASAYVDPDPGHSIQDIYEQIFAEPWSDASQSKDPLPPATMRGFAQN
Sbjct: 61  STSDPNSPSIFFGNASAYVDPDPGHSIQDIYEQIFAEPWSDASQSKDPLPPATMRGFAQN 120

Query: 121 AERIEKGMSAT------PEAVPVFKELVMEFGVCDRWFASVP 157
           AERIEKGMSAT      PEAVPVFKELVMEFGVCDRWFASVP
Sbjct: 121 AERIEKGMSATVMNXFKPEAVPVFKELVMEFGVCDRWFASVP 162

[Close]

BLAST of CmaCh08G010100 vs. ExPASy TrEMBL
Match: A0A6J1HKJ6 (LOW QUALITY PROTEIN: non-specific phospholipase C4-like OS=Cucurbita moschata OX=3662 GN=LOC111464383 PE=4 SV=1)

HSP 1 Score: 291.6 bits (745), Expect = 2.1e-75
Identity = 147/163 (90.18%), Postives = 151/163 (92.64%), Query Frame = 0


Query: 1   MSPAINATAAGDGKSTASPIKTIVILVQENRSFDHMLGWMKSLNPEIDGVTNENRFSNPV 60
           MSPA +ATA GDGKSTASPIKTIVILVQENRSFDHMLGWMKSLNPEIDGVT+ENRFS P+
Sbjct: 1   MSPATSATATGDGKSTASPIKTIVILVQENRSFDHMLGWMKSLNPEIDGVTDENRFSKPI 60

Query: 61  STSDPNSPSIFFGNASAYVDPDPGHSIQDIYEQIFAEPWSDASQSKDPLPPATMRGFAQN 120
           STSDPNSPSIFFGNASAYVDPDPGHSIQDI+EQIFAEPWSDASQSK PLP ATMRGFAQN
Sbjct: 61  STSDPNSPSIFFGNASAYVDPDPGHSIQDIHEQIFAEPWSDASQSKYPLPRATMRGFAQN 120

Query: 121 AERIEKGMSAT------PEAVPVFKELVMEFGVCDRWFASVPA 158
           AERIEKGMSAT      PEAVPVFKELVMEFGVCDRWFASV A
Sbjct: 121 AERIEKGMSATVMNGFKPEAVPVFKELVMEFGVCDRWFASVXA 163

[Close]

BLAST of CmaCh08G010100 vs. ExPASy TrEMBL
Match: A0A0A0LK27 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G119360 PE=4 SV=1)

HSP 1 Score: 241.5 bits (615), Expect = 2.5e-60
Identity = 124/164 (75.61%), Postives = 137/164 (83.54%), Query Frame = 0


Query: 1   MSPAINATAAG-DGKSTASPIKTIVILVQENRSFDHMLGWMKSLNPEIDGVTNENRFSNP 60
           MS  I A   G DGK++ +PIKTIVILVQENRSFDHMLGWMK+LNP+IDGVTN+ +FSNP
Sbjct: 1   MSLEITAGTDGRDGKASPNPIKTIVILVQENRSFDHMLGWMKTLNPKIDGVTNQTQFSNP 60

Query: 61  VSTSDPNSPSIFFGNASAYVDPDPGHSIQDIYEQIFAEPWSDASQSKDPLPPATMRGFAQ 120
           +STS PNS SI FGNASA+VDPDPGHSIQDIYEQ+FA PWSD      PLPP +M+GFAQ
Sbjct: 61  ISTSHPNSQSIPFGNASAFVDPDPGHSIQDIYEQVFAHPWSD---DLHPLPPPSMQGFAQ 120

Query: 121 NAERIEKGMSAT------PEAVPVFKELVMEFGVCDRWFASVPA 158
           NAERI+KGMSAT      PEAVPVFKELV EFGVCDRWFASVPA
Sbjct: 121 NAERIQKGMSATVMNGFKPEAVPVFKELVAEFGVCDRWFASVPA 161

[Close]

BLAST of CmaCh08G010100 vs. ExPASy TrEMBL
Match: A0A6J1DR24 (non-specific phospholipase C3-like OS=Momordica charantia OX=3673 GN=LOC111022768 PE=4 SV=1)

HSP 1 Score: 241.1 bits (614), Expect = 3.3e-60
Identity = 118/163 (72.39%), Postives = 138/163 (84.66%), Query Frame = 0


Query: 1   MSPAINATAAGDGKSTASPIKTIVILVQENRSFDHMLGWMKSLNPEIDGVTNENRFSNPV 60
           M+P I  +  GD KS ++PIKTIV+LVQENRSFDHM+GWMK+LNPEIDGV++E +FSNP+
Sbjct: 1   MAPEIGGSDGGDAKSASNPIKTIVVLVQENRSFDHMIGWMKTLNPEIDGVSDETQFSNPI 60

Query: 61  STSDPNSPSIFFGNASAYVDPDPGHSIQDIYEQIFAEPWSDASQSKDPLPPATMRGFAQN 120
           STSDPNSPS+ FGNASA+VDPDPGHSIQDI+EQIF +PWS A+ ++     ATMRGFAQN
Sbjct: 61  STSDPNSPSLHFGNASAFVDPDPGHSIQDIFEQIFGQPWSAAAAAQS--SSATMRGFAQN 120

Query: 121 AERIEKGMSAT------PEAVPVFKELVMEFGVCDRWFASVPA 158
           AERI KGMSAT      PEAVPVFKELV EFG+CDRWFA+VPA
Sbjct: 121 AERIGKGMSATVMNGFRPEAVPVFKELVSEFGLCDRWFAAVPA 161

[Close]

BLAST of CmaCh08G010100 vs. ExPASy TrEMBL
Match: A0A5D3BUA3 (Non-specific phospholipase C3-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold775G00010 PE=4 SV=1)

HSP 1 Score: 238.8 bits (608), Expect = 1.6e-59
Identity = 119/150 (79.33%), Postives = 129/150 (86.00%), Query Frame = 0


Query: 14  KSTASPIKTIVILVQENRSFDHMLGWMKSLNPEIDGVTNENRFSNPVSTSDPNSPSIFFG 73
           K +A PIKTIVILVQENRSFDHMLGWMK+LNP+IDGVTN+ +FSNP+STS PNS SI FG
Sbjct: 7   KPSAYPIKTIVILVQENRSFDHMLGWMKTLNPQIDGVTNQTQFSNPISTSHPNSQSIPFG 66

Query: 74  NASAYVDPDPGHSIQDIYEQIFAEPWSDASQSKDPLPPATMRGFAQNAERIEKGMSAT-- 133
           NASA+VDPDPGHSIQDIYEQ+FA PWSD      PLPP TM+GFAQNAERI+KGMSAT  
Sbjct: 67  NASAFVDPDPGHSIQDIYEQVFAHPWSD---DLHPLPPPTMQGFAQNAERIQKGMSATVM 126

Query: 134 ----PEAVPVFKELVMEFGVCDRWFASVPA 158
               PEAVPVFKELV EFGVCDRWFASVPA
Sbjct: 127 NGFKPEAVPVFKELVAEFGVCDRWFASVPA 153

[Close]

BLAST of CmaCh08G010100 vs. NCBI nr
Match: XP_023000253.1 (LOW QUALITY PROTEIN: non-specific phospholipase C3-like [Cucurbita maxima])

HSP 1 Score: 312.0 bits (798), Expect = 3.1e-81
Identity = 156/162 (96.30%), Postives = 156/162 (96.30%), Query Frame = 0


Query: 1   MSPAINATAAGDGKSTASPIKTIVILVQENRSFDHMLGWMKSLNPEIDGVTNENRFSNPV 60
           MSPAINATAAGDGKSTASPIKTIVILVQENRSFDHMLGWMKSLNPEIDGVTNENRFSNPV
Sbjct: 1   MSPAINATAAGDGKSTASPIKTIVILVQENRSFDHMLGWMKSLNPEIDGVTNENRFSNPV 60

Query: 61  STSDPNSPSIFFGNASAYVDPDPGHSIQDIYEQIFAEPWSDASQSKDPLPPATMRGFAQN 120
           STSDPNSPSIFFGNASAYVDPDPGHSIQDIYEQIFAEPWSDASQSKDPLPPATMRGFAQN
Sbjct: 61  STSDPNSPSIFFGNASAYVDPDPGHSIQDIYEQIFAEPWSDASQSKDPLPPATMRGFAQN 120

Query: 121 AERIEKGMSAT------PEAVPVFKELVMEFGVCDRWFASVP 157
           AERIEKGMSAT      PEAVPVFKELVMEFGVCDRWFASVP
Sbjct: 121 AERIEKGMSATVMNXFKPEAVPVFKELVMEFGVCDRWFASVP 162

[Close]

BLAST of CmaCh08G010100 vs. NCBI nr
Match: KAG7026102.1 (Non-specific phospholipase C3, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 302.0 bits (772), Expect = 3.2e-78
Identity = 150/163 (92.02%), Postives = 155/163 (95.09%), Query Frame = 0


Query: 1   MSPAINATAAGDGKSTASPIKTIVILVQENRSFDHMLGWMKSLNPEIDGVTNENRFSNPV 60
           MSPAI+ATA GDGKSTASPIKTIVILVQENRSFDHMLGWMKSLNPEIDGV+NENRFSNP+
Sbjct: 1   MSPAISATATGDGKSTASPIKTIVILVQENRSFDHMLGWMKSLNPEIDGVSNENRFSNPI 60

Query: 61  STSDPNSPSIFFGNASAYVDPDPGHSIQDIYEQIFAEPWSDASQSKDPLPPATMRGFAQN 120
           STSDPNSPSIFFGNASAYVDPDPGHSIQD++EQIFAEPWSDASQSKDPLP ATMRGFAQN
Sbjct: 61  STSDPNSPSIFFGNASAYVDPDPGHSIQDVHEQIFAEPWSDASQSKDPLPLATMRGFAQN 120

Query: 121 AERIEKGMSAT------PEAVPVFKELVMEFGVCDRWFASVPA 158
           AERIEKGMSAT      PEAVPVFKELVMEFGVCDRWFASVPA
Sbjct: 121 AERIEKGMSATVMNGFKPEAVPVFKELVMEFGVCDRWFASVPA 163

[Close]

BLAST of CmaCh08G010100 vs. NCBI nr
Match: XP_022964348.1 (LOW QUALITY PROTEIN: non-specific phospholipase C4-like [Cucurbita moschata])

HSP 1 Score: 291.6 bits (745), Expect = 4.4e-75
Identity = 147/163 (90.18%), Postives = 151/163 (92.64%), Query Frame = 0


Query: 1   MSPAINATAAGDGKSTASPIKTIVILVQENRSFDHMLGWMKSLNPEIDGVTNENRFSNPV 60
           MSPA +ATA GDGKSTASPIKTIVILVQENRSFDHMLGWMKSLNPEIDGVT+ENRFS P+
Sbjct: 1   MSPATSATATGDGKSTASPIKTIVILVQENRSFDHMLGWMKSLNPEIDGVTDENRFSKPI 60

Query: 61  STSDPNSPSIFFGNASAYVDPDPGHSIQDIYEQIFAEPWSDASQSKDPLPPATMRGFAQN 120
           STSDPNSPSIFFGNASAYVDPDPGHSIQDI+EQIFAEPWSDASQSK PLP ATMRGFAQN
Sbjct: 61  STSDPNSPSIFFGNASAYVDPDPGHSIQDIHEQIFAEPWSDASQSKYPLPRATMRGFAQN 120

Query: 121 AERIEKGMSAT------PEAVPVFKELVMEFGVCDRWFASVPA 158
           AERIEKGMSAT      PEAVPVFKELVMEFGVCDRWFASV A
Sbjct: 121 AERIEKGMSATVMNGFKPEAVPVFKELVMEFGVCDRWFASVXA 163

[Close]

BLAST of CmaCh08G010100 vs. NCBI nr
Match: KAG6593769.1 (Non-specific phospholipase C3, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 278.1 bits (710), Expect = 5.0e-71
Identity = 142/163 (87.12%), Postives = 146/163 (89.57%), Query Frame = 0


Query: 1   MSPAINATAAGDGKSTASPIKTIVILVQENRSFDHMLGWMKSLNPEIDGVTNENRFSNPV 60
           MSPAI+A        TASPIKTIVILVQENRSFDHMLGWMKSLNPEIDGV+NENRFSNP+
Sbjct: 1   MSPAISA--------TASPIKTIVILVQENRSFDHMLGWMKSLNPEIDGVSNENRFSNPI 60

Query: 61  STSDPNSPSIFFGNASAYVDPDPGHSIQDIYEQIFAEPWSDASQSKDPLPPATMRGFAQN 120
           STSDPNSPSIFFGNASAYVDPDPGH IQDI+EQIFAEPWSDASQSKDPLP ATMRGFAQN
Sbjct: 61  STSDPNSPSIFFGNASAYVDPDPGHLIQDIHEQIFAEPWSDASQSKDPLPLATMRGFAQN 120

Query: 121 AERIEKGMSAT------PEAVPVFKELVMEFGVCDRWFASVPA 158
            ERIEKGMSAT      PEAVPVFKELVMEFGVCDRWFASVPA
Sbjct: 121 TERIEKGMSATVMNGFKPEAVPVFKELVMEFGVCDRWFASVPA 155

[Close]

BLAST of CmaCh08G010100 vs. NCBI nr
Match: XP_038875468.1 (non-specific phospholipase C3-like [Benincasa hispida])

HSP 1 Score: 255.8 bits (652), Expect = 2.7e-64
Identity = 128/164 (78.05%), Postives = 143/164 (87.20%), Query Frame = 0


Query: 1   MSPAINATAAG-DGKSTASPIKTIVILVQENRSFDHMLGWMKSLNPEIDGVTNENRFSNP 60
           MSP I  T  G DG+++A+PIKTIVILVQENRSFDHMLGWMK+LNP+IDGVTN+++FSNP
Sbjct: 1   MSPEITPTGGGDDGEASANPIKTIVILVQENRSFDHMLGWMKTLNPQIDGVTNQSQFSNP 60

Query: 61  VSTSDPNSPSIFFGNASAYVDPDPGHSIQDIYEQIFAEPWSDASQSKDPLPPATMRGFAQ 120
           +STSDPNSPS+ FGNASA+VDPDPGHSIQDIYEQIFA+PWSD      PLPP TM+GFAQ
Sbjct: 61  ISTSDPNSPSLPFGNASAFVDPDPGHSIQDIYEQIFAQPWSD---DLHPLPPPTMQGFAQ 120

Query: 121 NAERIEKGMSAT------PEAVPVFKELVMEFGVCDRWFASVPA 158
           NAERI+KGMSAT      PEAVPVFKELV EFGVCDRWFASVPA
Sbjct: 121 NAERIQKGMSATVMNGFKPEAVPVFKELVAEFGVCDRWFASVPA 161

[Close]

BLAST of CmaCh08G010100 vs. TAIR 10
Match: AT3G03520.1 (non-specific phospholipase C3 )

HSP 1 Score: 194.9 bits (494), Expect = 5.2e-50
Identity = 100/160 (62.50%), Postives = 115/160 (71.88%), Query Frame = 0


Query: 5   INATAAGDGKSTASPIKTIVILVQENRSFDHMLGWMKSLNPEIDGVTNENRFSNPVSTSD 64
           +  T++G G S+ASPIKTIV+LVQENRSFDHMLGW K LNPEIDGV+     SNP+STSD
Sbjct: 2   VEETSSGGG-SSASPIKTIVVLVQENRSFDHMLGWFKELNPEIDGVSESEPRSNPLSTSD 61

Query: 65  PNSPSIFFGNASAYVDPDPGHSIQDIYEQIFAEPWSDASQSKDPLPPATMRGFAQNAERI 124
           PNS  IFFG  S  +DPDPGHS Q IYEQ+F +P+SD S    P P   M GF QNAE I
Sbjct: 62  PNSAQIFFGKESQNIDPDPGHSFQAIYEQVFGKPFSDES----PYPDPKMNGFVQNAEAI 121

Query: 125 EKGMSA-------TPEAVPVFKELVMEFGVCDRWFASVPA 158
            KGMS         PE +PVFKELV EF VCDRWF+S+P+
Sbjct: 122 TKGMSEKVVMQGFPPEKLPVFKELVQEFAVCDRWFSSLPS 156

[Close]

BLAST of CmaCh08G010100 vs. TAIR 10
Match: AT3G03530.1 (non-specific phospholipase C4 )

HSP 1 Score: 183.3 bits (464), Expect = 1.6e-46
Identity = 93/151 (61.59%), Postives = 108/151 (71.52%), Query Frame = 0


Query: 13  GKSTASPIKTIVILVQENRSFDHMLGWMKSLNPEIDGVTNENRFSNPVSTSDPNSPSIFF 72
           G S + PIKTIV+LVQENRSFDH LGW K LN EIDGVT  +  SN VS+SD NS  + F
Sbjct: 7   GGSGSYPIKTIVVLVQENRSFDHTLGWFKELNREIDGVTKSDPKSNTVSSSDTNSLRVVF 66

Query: 73  GNASAYVDPDPGHSIQDIYEQIFAEPWSDASQSKDPLPPATMRGFAQNAERIEKGMSAT- 132
           G+ S YV+PDPGHSIQDIYEQ+F +PW       +P  P  M GFAQNAER +KGMS+  
Sbjct: 67  GDQSQYVNPDPGHSIQDIYEQVFGKPWDSGKPDPNPGHP-NMSGFAQNAERNKKGMSSAV 126

Query: 133 -----PEAVPVFKELVMEFGVCDRWFASVPA 158
                P A+PV+KELV  F +CDRWFASVPA
Sbjct: 127 MNGFKPNALPVYKELVQNFAICDRWFASVPA 156

[Close]

BLAST of CmaCh08G010100 vs. TAIR 10
Match: AT3G03540.1 (non-specific phospholipase C5 )

HSP 1 Score: 178.7 bits (452), Expect = 3.9e-45
Identity = 92/149 (61.74%), Postives = 109/149 (73.15%), Query Frame = 0


Query: 15  STASPIKTIVILVQENRSFDHMLGWMKSLNPEIDGVTNENRFSNPVSTSDPNSPSIFFGN 74
           S + PIKTIV+LVQENRSFDH LGW K LN EIDGV   ++  NP  +SD NS ++ FG+
Sbjct: 8   SESYPIKTIVVLVQENRSFDHTLGWFKELNREIDGVMKSDQKFNPGFSSDLNSHNVVFGD 67

Query: 75  ASAYVDPDPGHSIQDIYEQIFAEPWSDASQSKDPLP-PATMRGFAQNAERIEKGMSAT-- 134
            S YVDP+PGHSI+DIYEQ+F +PW   S   DP P PATM GFAQNAER  KGMS+   
Sbjct: 68  QSQYVDPNPGHSIRDIYEQVFGKPWD--SGHPDPNPGPATMSGFAQNAERKMKGMSSAVM 127

Query: 135 ----PEAVPVFKELVMEFGVCDRWFASVP 157
               P+A+PV+KELV  F +CDRWFASVP
Sbjct: 128 NGFKPDALPVYKELVQNFAICDRWFASVP 154

[Close]

BLAST of CmaCh08G010100 vs. TAIR 10
Match: AT2G26870.1 (non-specific phospholipase C2 )

HSP 1 Score: 148.3 bits (373), Expect = 5.6e-36
Identity = 85/148 (57.43%), Postives = 97/148 (65.54%), Query Frame = 0


Query: 18  SPIKTIVILVQENRSFDHMLGWMKSLNPEIDGVTNENRFSNPVSTSDPNSPSIFFGNASA 77
           SPIKTIV++V ENRSFDHMLGWMK LNPEI+GV      SNPVS SDP+S  I FG+ S 
Sbjct: 25  SPIKTIVVVVMENRSFDHMLGWMKKLNPEINGVDGSE--SNPVSVSDPSSRKIKFGSGSH 84

Query: 78  YVDPDPGHSIQDIYEQIFAEPWSDASQSKDPLPPATMRGFAQNA--ERIEKGMSAT---- 137
           YVDPDPGHS Q I EQ+F    +D S     + P  M GF Q A  E     MSA+    
Sbjct: 85  YVDPDPGHSFQAIREQVFGS--NDTS-----MDPPPMNGFVQQAYSEDPSGNMSASVMNG 144

Query: 138 --PEAVPVFKELVMEFGVCDRWFASVPA 158
             P+ VPV+K LV EF V DRWFASVP+
Sbjct: 145 FEPDKVPVYKSLVSEFAVFDRWFASVPS 163

[Close]

BLAST of CmaCh08G010100 vs. TAIR 10
Match: AT1G07230.1 (non-specific phospholipase C1 )

HSP 1 Score: 144.1 bits (362), Expect = 1.1e-34
Identity = 77/144 (53.47%), Postives = 93/144 (64.58%), Query Frame = 0


Query: 19  PIKTIVILVQENRSFDHMLGWMKSLNPEIDGVTNENRFSNPVSTSDPNSPSIFFGNASAY 78
           PIKTIV++V ENRSFDH+LGW+KS  PEIDG+T +   SNP++ SDPNS  IF  + + +
Sbjct: 37  PIKTIVVVVMENRSFDHILGWLKSTRPEIDGLTGKE--SNPLNVSDPNSKKIFVSDDAVF 96

Query: 79  VDPDPGHSIQDIYEQIFAEPWSDASQSKDPLPPATMRGFAQNAERIEKGMSAT------P 138
           VD DPGHS Q I EQIF         S D      M GFAQ +E +E GM+        P
Sbjct: 97  VDMDPGHSFQAIREQIFG--------SNDTSGDPKMNGFAQQSESMEPGMAKNVMSGFKP 156

Query: 139 EAVPVFKELVMEFGVCDRWFASVP 157
           E +PV+ EL  EFGV DRWFASVP
Sbjct: 157 EVLPVYTELANEFGVFDRWFASVP 170

The following BLAST results are available for this feature:

BLAST of CmaCh08G010100 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SRQ6	7.3e-49	62.50	Non-specific phospholipase C3 OS=Arabidopsis thaliana OX=3702 GN=NPC3 PE=1 SV=1	[more]
Q9SRQ7	2.2e-45	61.59	Non-specific phospholipase C4 OS=Arabidopsis thaliana OX=3702 GN=NPC4 PE=1 SV=1	[more]
Q9S816	5.4e-44	61.74	Non-specific phospholipase C5 OS=Arabidopsis thaliana OX=3702 GN=NPC5 PE=1 SV=1	[more]
O81020	7.9e-35	57.43	Non-specific phospholipase C2 OS=Arabidopsis thaliana OX=3702 GN=NPC2 PE=2 SV=1	[more]
Q8L7Y9	1.5e-33	53.47	Non-specific phospholipase C1 OS=Arabidopsis thaliana OX=3702 GN=NPC1 PE=2 SV=1	[more]

BLAST of CmaCh08G010100 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1KM42	1.5e-81	96.30	LOW QUALITY PROTEIN: non-specific phospholipase C3-like OS=Cucurbita maxima OX=3...	[more]
A0A6J1HKJ6	2.1e-75	90.18	LOW QUALITY PROTEIN: non-specific phospholipase C4-like OS=Cucurbita moschata OX...	[more]
A0A0A0LK27	2.5e-60	75.61	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G119360 PE=4 SV=1	[more]
A0A6J1DR24	3.3e-60	72.39	non-specific phospholipase C3-like OS=Momordica charantia OX=3673 GN=LOC11102276...	[more]
A0A5D3BUA3	1.6e-59	79.33	Non-specific phospholipase C3-like OS=Cucumis melo var. makuwa OX=1194695 GN=E56...	[more]

BLAST of CmaCh08G010100 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023000253.1	3.1e-81	96.30	LOW QUALITY PROTEIN: non-specific phospholipase C3-like [Cucurbita maxima]	[more]
KAG7026102.1	3.2e-78	92.02	Non-specific phospholipase C3, partial [Cucurbita argyrosperma subsp. argyrosper...	[more]
XP_022964348.1	4.4e-75	90.18	LOW QUALITY PROTEIN: non-specific phospholipase C4-like [Cucurbita moschata]	[more]
KAG6593769.1	5.0e-71	87.12	Non-specific phospholipase C3, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_038875468.1	2.7e-64	78.05	non-specific phospholipase C3-like [Benincasa hispida]	[more]

BLAST of CmaCh08G010100 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G03520.1	5.2e-50	62.50	non-specific phospholipase C3	[more]
AT3G03530.1	1.6e-46	61.59	non-specific phospholipase C4	[more]
AT3G03540.1	3.9e-45	61.74	non-specific phospholipase C5	[more]
AT2G26870.1	5.6e-36	57.43	non-specific phospholipase C2	[more]
AT1G07230.1	1.1e-34	53.47	non-specific phospholipase C1	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR007312	Phosphoesterase	PFAM	PF04185	Phosphoesterase	coord: 20..158 e-value: 1.8E-26 score: 93.2
IPR007312	Phosphoesterase	PANTHER	PTHR31956	NON-SPECIFIC PHOSPHOLIPASE C4-RELATED	coord: 13..158
IPR017850	Alkaline-phosphatase-like, core domain superfamily	GENE3D	3.40.720.10	Alkaline Phosphatase, subunit A	coord: 11..162 e-value: 6.5E-22 score: 80.6
None	No IPR available	PANTHER	PTHR31956:SF32	NON-SPECIFIC PHOSPHOLIPASE C3	coord: 13..158

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmaCh08G010100.1	CmaCh08G010100.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0048229	gametophyte development
biological_process	GO:0009395	phospholipid catabolic process
biological_process	GO:0048364	root development
cellular_component	GO:0009507	chloroplast
molecular_function	GO:0004629	phospholipase C activity
molecular_function	GO:0003824	catalytic activity
molecular_function	GO:0016788	hydrolase activity, acting on ester bonds