CmaCh08G009830 (gene) Cucurbita maxima (Rimu) v1.1

Overview


Name	CmaCh08G009830
Type	gene
Organism	Cucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Description	protein NUCLEAR FUSION DEFECTIVE 4-like isoform X1
Location	Cma_Chr08: 6150201 .. 6152924 (-)
RNA-Seq Expression	CmaCh08G009830
Synteny	CmaCh08G009830

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AAAGAATTTCCAAAATTGCTAATAAAAGCATATATGGAATTGACGTTGGTGATATCAATCAAAATCTGATTGGATCACCTTGTAAGCTATGTTTGGCATCTTTCAAGCGTGCTTTAAATGAAAAAGGACAATTTTTGTTACACCAAATCCTTCAACCATCGTTGGATTCAACAACTTCCACCCACCCTTATGGTATGGTCTACCGGCCACCCTATTCACCAAGCGCTTTTTCAATCACTATTCTCCTCAGGGCAGCGCCATGAAAATAAAACACAGAAAACCTCAAAATTTTCCCCATCTTCCCGTCACAAAACAGAGAAGGATGATGAACCAACCTGGAAAATGGGTGGCGCTGATGGCGGCCATATGGATTCAGGCATTTACCGGCACAAATTTTGATTTCCCCTCTTATTCTTCCAATCTGAAGGCGGCTTTGGGGATGTCGCAGGTAGAGCTCAACTATCTGGCGGTGGCTTCTGACGTCGGTAAGGCCTTTGGGTGGTGCTCCGGCATCGCTCTCTTGTATTTTCCTTCGTGGGTGGTGATGCTCATGGCTGCTTCCATGGGGTTTCTTGGCTATGGCCTTCAATGGCTTCTCCTTCAAGGGATTGTATCTCTGCCTTACTCCATGGTTAGTTTCCCTTCTCATCTCTTCTCTATTGTTTACACCAAATCCACGTTGACCTGAATTGGATACATTTCCTGACAAATTGGGGAAAAGGGTTTTCTTAACTTAATAGCAGTATGAATAGATCCACACAAATATTCATCATCAATTTCCAACTAAATTGTCACATATTTCATACCCTTTTCATTGAAACTATCAAGAATGAAGATAGGTTCGAGGTTGGGGGAGGCAAACAAATTGGAATGATTGCTCTCATTTTCCTGCAGGTATATCTTCTCTGCTTGTTGGCTGGATGTAGTATTTGCTGGTTTAATACGATCTGCTATGTCTCCTGCATCCAAAAGTTCCCAGCCAATCGCGCGCTTGCACTGTCCTTGATTGTCAGCTTCAATGGCGTCAGTGCAGCTCTTTACACCCTAATGACCAATGCAGTAGACCCTAATGATGCAAGCTTGTACCTTCTCCTAAATGCATTGGTTCCTCTCATAATCTCTGTCGTTGCCTTCTTCCCAATCCTTCATCAGCCACCGGCTCAGCCGTCGTCTGCCGATGCCACCCGCAATGACTCCCTCACTTTCATCTGCTTATACATCACCGCAGTTATCACAGGCCTGTATCTAATCACCTTCAATTCCACGCCTTCCAGCAAGTACGGGGCCCAAATTCTGTTAGCTGGGGCTTTTGCCCTGTTACTAGTTCCTCTTTGTCTGCCAGGAATTTGGAGTACTCACAAATGGTTGTTTCGGATCGCATCCACTGGCTTGAGTAGTGTTCTTCACTTTAGATTCAACCTTGTCAATCATGAGCTTCAACAAGAGCTCATCAATGTTGAATCAGAAAGAAGTGGCACGAAGGTCATCACCCCTTGTGATTATAAAGAGAAGGAAAGCATTTCAAGGAAGATGATGGGGAAAGAGAATTTGACAGTTCTTGAAGAAGAGCATTCAGCTAAAATGCTCGTACGACGCTTGGACTTTTGGCTCTATTTTGTTGCATACTTTTGTGGTGGGACAATTGGGTTGGTTTACAGCAACAATCTTGGGCAAATCTCGCAGTCACTAGGATACAGTTCGTTAACAAGCTCACTTGTAACACTCTACTCGTCTTGCTCCTTCTTTGGGCGGCTAATCTCAGCAGGCCCAGATTTCATGCGTGAGTATGTAGTAGCAGCAAACGGGTCAATCCTTCATATATATTCATTTTCCATGAATGATTTAGCTTCAGATAACTCATAACTTTTCCATTTGCATTAGATCTTTATGCTATAAGTTAGTATAGCAAATGCAAAATTGGTTATTCTACATACATATATTCATTTGTCACCAAAACATGAAGTTCAGAAAGCTTGAAAGTTTTCATTTGTATCAGAAACTAGGAAGTATGGGTTCATATTCCTTCTTCTAGATTTCATGTAGTGACATCAAAAGGTTCATATCTAGATGCAGGATTCATCAGTCATTGAAACTTTCCATTTTGCATTAGAAATTTTGGCATCATATTCAATTCTTGTAAACTTCCAAAACACAATCAGTAATCAACAACAGAGAAAAACAATGCTAGTGAACTGATTAAACTTCGCACCTGTGCAGAAAGGTGCACTTCGCCAGAACTGGATGGCTAGCCCTAGCACTAGTGCCAAATCCAATCGCCTTCATTCTACTCGCTGCATCAGGCAGCGAGATCGCATTACAAATTGGCACTGGCTTGATCGGTCTAAGCTCGGGATTCATGTTCTCAGCCTCGGTCTCAATCACATCAGAGTTATTCGGACCAAACAGCTCAGGCGTCAATCACAACATCCTCATCACCAACATCCCTCTCGGCTCGTTCCTCTACGGCGTCCTGGCGGCAGTTTCATATGACTCAACTGCCGGAAGTTCAAACCAAACTGCGGCGACGCTTGGGGACGTGGTGGTGTGCATCGGCCGGAAATGCTATCTACAAACCTTCATATGGTGGGCTTGCATCTCCATCGTCGGCCTCGCTTCTTGCTTCTTGCTTTTCCGACGGACGAAACCGGCATACGACCGCCATTACGAGAGTAACCCAAGCAAGACACAGCCATTTTAGATCAGATACTGAACTTTACAAAAAAA

mRNA sequence

AAAGAATTTCCAAAATTGCTAATAAAAGCATATATGGAATTGACGTTGGTGATATCAATCAAAATCTGATTGGATCACCTTGTAAGCTATGTTTGGCATCTTTCAAGCGTGCTTTAAATGAAAAAGGACAATTTTTGTTACACCAAATCCTTCAACCATCGTTGGATTCAACAACTTCCACCCACCCTTATGGTATGGTCTACCGGCCACCCTATTCACCAAGCGCTTTTTCAATCACTATTCTCCTCAGGGCAGCGCCATGAAAATAAAACACAGAAAACCTCAAAATTTTCCCCATCTTCCCGTCACAAAACAGAGAAGGATGATGAACCAACCTGGAAAATGGGTGGCGCTGATGGCGGCCATATGGATTCAGGCATTTACCGGCACAAATTTTGATTTCCCCTCTTATTCTTCCAATCTGAAGGCGGCTTTGGGGATGTCGCAGGTAGAGCTCAACTATCTGGCGGTGGCTTCTGACGTCGGTAAGGCCTTTGGGTGGTGCTCCGGCATCGCTCTCTTGTATTTTCCTTCGTGGGTGGTGATGCTCATGGCTGCTTCCATGGGGTTTCTTGGCTATGGCCTTCAATGGCTTCTCCTTCAAGGGATTGTATCTCTGCCTTACTCCATGGTATATCTTCTCTGCTTGTTGGCTGGATGTAGTATTTGCTGGTTTAATACGATCTGCTATGTCTCCTGCATCCAAAAGTTCCCAGCCAATCGCGCGCTTGCACTGTCCTTGATTGTCAGCTTCAATGGCGTCAGTGCAGCTCTTTACACCCTAATGACCAATGCAGTAGACCCTAATGATGCAAGCTTGTACCTTCTCCTAAATGCATTGGTTCCTCTCATAATCTCTGTCGTTGCCTTCTTCCCAATCCTTCATCAGCCACCGGCTCAGCCGTCGTCTGCCGATGCCACCCGCAATGACTCCCTCACTTTCATCTGCTTATACATCACCGCAGTTATCACAGGCCTGTATCTAATCACCTTCAATTCCACGCCTTCCAGCAAGTACGGGGCCCAAATTCTGTTAGCTGGGGCTTTTGCCCTGTTACTAGTTCCTCTTTGTCTGCCAGGAATTTGGAGTACTCACAAATGGTTGTTTCGGATCGCATCCACTGGCTTGAGTAGTGTTCTTCACTTTAGATTCAACCTTGTCAATCATGAGCTTCAACAAGAGCTCATCAATGTTGAATCAGAAAGAAGTGGCACGAAGGTCATCACCCCTTGTGATTATAAAGAGAAGGAAAGCATTTCAAGGAAGATGATGGGGAAAGAGAATTTGACAGTTCTTGAAGAAGAGCATTCAGCTAAAATGCTCGTACGACGCTTGGACTTTTGGCTCTATTTTGTTGCATACTTTTGTGGTGGGACAATTGGGTTGGTTTACAGCAACAATCTTGGGCAAATCTCGCAGTCACTAGGATACAGTTCGTTAACAAGCTCACTTGTAACACTCTACTCGTCTTGCTCCTTCTTTGGGCGGCTAATCTCAGCAGGCCCAGATTTCATGCGTGAAAAGGTGCACTTCGCCAGAACTGGATGGCTAGCCCTAGCACTAGTGCCAAATCCAATCGCCTTCATTCTACTCGCTGCATCAGGCAGCGAGATCGCATTACAAATTGGCACTGGCTTGATCGGTCTAAGCTCGGGATTCATGTTCTCAGCCTCGGTCTCAATCACATCAGAGTTATTCGGACCAAACAGCTCAGGCGTCAATCACAACATCCTCATCACCAACATCCCTCTCGGCTCGTTCCTCTACGGCGTCCTGGCGGCAGTTTCATATGACTCAACTGCCGGAAGTTCAAACCAAACTGCGGCGACGCTTGGGGACGTGGTGGTGTGCATCGGCCGGAAATGCTATCTACAAACCTTCATATGGTGGGCTTGCATCTCCATCGTCGGCCTCGCTTCTTGCTTCTTGCTTTTCCGACGGACGAAACCGGCATACGACCGCCATTACGAGAGTAACCCAAGCAAGACACAGCCATTTTAGATCAGATACTGAACTTTACAAAAAAA

Coding sequence (CDS)

ATGAAAATAAAACACAGAAAACCTCAAAATTTTCCCCATCTTCCCGTCACAAAACAGAGAAGGATGATGAACCAACCTGGAAAATGGGTGGCGCTGATGGCGGCCATATGGATTCAGGCATTTACCGGCACAAATTTTGATTTCCCCTCTTATTCTTCCAATCTGAAGGCGGCTTTGGGGATGTCGCAGGTAGAGCTCAACTATCTGGCGGTGGCTTCTGACGTCGGTAAGGCCTTTGGGTGGTGCTCCGGCATCGCTCTCTTGTATTTTCCTTCGTGGGTGGTGATGCTCATGGCTGCTTCCATGGGGTTTCTTGGCTATGGCCTTCAATGGCTTCTCCTTCAAGGGATTGTATCTCTGCCTTACTCCATGGTATATCTTCTCTGCTTGTTGGCTGGATGTAGTATTTGCTGGTTTAATACGATCTGCTATGTCTCCTGCATCCAAAAGTTCCCAGCCAATCGCGCGCTTGCACTGTCCTTGATTGTCAGCTTCAATGGCGTCAGTGCAGCTCTTTACACCCTAATGACCAATGCAGTAGACCCTAATGATGCAAGCTTGTACCTTCTCCTAAATGCATTGGTTCCTCTCATAATCTCTGTCGTTGCCTTCTTCCCAATCCTTCATCAGCCACCGGCTCAGCCGTCGTCTGCCGATGCCACCCGCAATGACTCCCTCACTTTCATCTGCTTATACATCACCGCAGTTATCACAGGCCTGTATCTAATCACCTTCAATTCCACGCCTTCCAGCAAGTACGGGGCCCAAATTCTGTTAGCTGGGGCTTTTGCCCTGTTACTAGTTCCTCTTTGTCTGCCAGGAATTTGGAGTACTCACAAATGGTTGTTTCGGATCGCATCCACTGGCTTGAGTAGTGTTCTTCACTTTAGATTCAACCTTGTCAATCATGAGCTTCAACAAGAGCTCATCAATGTTGAATCAGAAAGAAGTGGCACGAAGGTCATCACCCCTTGTGATTATAAAGAGAAGGAAAGCATTTCAAGGAAGATGATGGGGAAAGAGAATTTGACAGTTCTTGAAGAAGAGCATTCAGCTAAAATGCTCGTACGACGCTTGGACTTTTGGCTCTATTTTGTTGCATACTTTTGTGGTGGGACAATTGGGTTGGTTTACAGCAACAATCTTGGGCAAATCTCGCAGTCACTAGGATACAGTTCGTTAACAAGCTCACTTGTAACACTCTACTCGTCTTGCTCCTTCTTTGGGCGGCTAATCTCAGCAGGCCCAGATTTCATGCGTGAAAAGGTGCACTTCGCCAGAACTGGATGGCTAGCCCTAGCACTAGTGCCAAATCCAATCGCCTTCATTCTACTCGCTGCATCAGGCAGCGAGATCGCATTACAAATTGGCACTGGCTTGATCGGTCTAAGCTCGGGATTCATGTTCTCAGCCTCGGTCTCAATCACATCAGAGTTATTCGGACCAAACAGCTCAGGCGTCAATCACAACATCCTCATCACCAACATCCCTCTCGGCTCGTTCCTCTACGGCGTCCTGGCGGCAGTTTCATATGACTCAACTGCCGGAAGTTCAAACCAAACTGCGGCGACGCTTGGGGACGTGGTGGTGTGCATCGGCCGGAAATGCTATCTACAAACCTTCATATGGTGGGCTTGCATCTCCATCGTCGGCCTCGCTTCTTGCTTCTTGCTTTTCCGACGGACGAAACCGGCATACGACCGCCATTACGAGAGTAACCCAAGCAAGACACAGCCATTTTAG

Protein sequence

MKIKHRKPQNFPHLPVTKQRRMMNQPGKWVALMAAIWIQAFTGTNFDFPSYSSNLKAALGMSQVELNYLAVASDVGKAFGWCSGIALLYFPSWVVMLMAASMGFLGYGLQWLLLQGIVSLPYSMVYLLCLLAGCSICWFNTICYVSCIQKFPANRALALSLIVSFNGVSAALYTLMTNAVDPNDASLYLLLNALVPLIISVVAFFPILHQPPAQPSSADATRNDSLTFICLYITAVITGLYLITFNSTPSSKYGAQILLAGAFALLLVPLCLPGIWSTHKWLFRIASTGLSSVLHFRFNLVNHELQQELINVESERSGTKVITPCDYKEKESISRKMMGKENLTVLEEEHSAKMLVRRLDFWLYFVAYFCGGTIGLVYSNNLGQISQSLGYSSLTSSLVTLYSSCSFFGRLISAGPDFMREKVHFARTGWLALALVPNPIAFILLAASGSEIALQIGTGLIGLSSGFMFSASVSITSELFGPNSSGVNHNILITNIPLGSFLYGVLAAVSYDSTAGSSNQTAATLGDVVVCIGRKCYLQTFIWWACISIVGLASCFLLFRRTKPAYDRHYESNPSKTQPF

Homology

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BLAST of CmaCh08G009830 vs. ExPASy Swiss-Prot
Match: F4I9E1 (Protein NUCLEAR FUSION DEFECTIVE 4 OS=Arabidopsis thaliana OX=3702 GN=NFD4 PE=3 SV=1)

HSP 1 Score: 552.4 bits (1422), Expect = 6.2e-156
Identity = 311/581 (53.53%), Postives = 401/581 (69.02%), Query Frame = 0


Query: 5   HRKPQNFPHLPVTKQRRMMNQPGKWVALMAAIWIQAFTGTNFDFPSYSSNLKAALGMSQV 64
           HRK     HLP    R M  +  KW  L+AAIWIQA TGTNFDF +YSS+LK+ LG+SQV
Sbjct: 27  HRK-----HLP--PLRTMFGRWRKWTVLVAAIWIQASTGTNFDFSAYSSHLKSVLGISQV 86

Query: 65  ELNYLAVASDVGKAFGWCSGIALLYFPSWVVMLMAASMGFLGYGLQWLLLQGIVSLPYSM 124
            LNYLAVASD+GKAFGW SGIAL YFP  VV+  AA+MGF+GYG+QWL++  I++LPYS+
Sbjct: 87  RLNYLAVASDLGKAFGWSSGIALGYFPLSVVLFAAAAMGFVGYGVQWLVITNIITLPYSL 146

Query: 125 VYLLCLLAGCSICWFNTICYVSCIQKFPANRALALSLIVSFNGVSAALYTLMTNAVDPND 184
           V+L CLLAG SICWFNT C++ CI+ FP NRALALSL VSFNG+SAALY+L  NA++P+ 
Sbjct: 147 VFLCCLLAGLSICWFNTACFILCIRHFPNNRALALSLTVSFNGISAALYSLAFNAINPSS 206

Query: 185 ASLYLLLNALVPLIISVVAFFPILHQPPAQPS-SADATRNDSLTFICLYITAVITGLYLI 244
           ++LYLLLN+LVPL++S  A +P+L +P    +   D+ R+DS  F  L + AVIT  +L+
Sbjct: 207 SNLYLLLNSLVPLVVSFAALYPVLTKPSLDTTPDYDSRRHDSHVFTILNVLAVITSFHLL 266

Query: 245 TFNSTPSSKYGAQILLAGAFALLLVPLCLPGIWSTHKWLFRIASTGLSSVLHFRFNLVNH 304
             +S+ SS   A++   GA  LL+ PLC P +     +   + +  L           NH
Sbjct: 267 LSSSSTSS---ARLNFIGAVVLLVFPLCAPLLVYARDYFLPVINARL-----------NH 326

Query: 305 ELQ-QELINV-ESERSGTKVITPCDYKEKESISRKMMGKENLTV-LEEEHSAKMLVRRLD 364
           E     ++N+ E +   T V +   Y+   +       KE  TV L +EHS ++L+ RL+
Sbjct: 327 ESSGYVMLNIDELKNQKTSVSSKTGYEHMGT------AKEGNTVRLGDEHSFRLLISRLE 386

Query: 365 FWLYFVAYFCGGTIGLVYSNNLGQISQSLGYSSLTSSLVTLYSSCSFFGRLISAGPDFMR 424
           FWLY++AYFCGGTIGLVYSNNLGQI+QSLG +S T  LVT+YSS SFFGRL+SA PDFM 
Sbjct: 387 FWLYYIAYFCGGTIGLVYSNNLGQIAQSLGQNSTT--LVTIYSSFSFFGRLLSAAPDFMH 446

Query: 425 EKVHFARTGWLALALVPNPIAFILLAASGS-EIALQIGTGLIGLSSGFMFSASVSITSEL 484
           ++    RTGW A+AL+P PIAF LLA S S + ALQ  T LIGLSSGF+F+A+VSITS+L
Sbjct: 447 KRFRLTRTGWFAIALLPTPIAFFLLAVSSSQQTALQTATALIGLSSGFIFAAAVSITSDL 506

Query: 485 FGPNSSGVNHNILITNIPLGSFLYGVLAAVSYDSTAGSSNQTAATLGDVVVCIGRKCYLQ 544
           FGPNS GVNHNILITNIP+GS LYG +AA  Y+  A +S      + D +VCIGR CY +
Sbjct: 507 FGPNSVGVNHNILITNIPIGSLLYGYIAASIYE--ANASPDITPIVSDSIVCIGRDCYFK 566

Query: 545 TFIWWACISIVGLASCFLLFRRTKPAYDRHYESNPSKTQPF 581
           TF++W C+SI+G+ S   L+ RTKP Y R  +   S T  +
Sbjct: 567 TFVFWGCLSILGVVSSLSLYIRTKPVYHRLEQDKVSLTSSY 576

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BLAST of CmaCh08G009830 vs. ExPASy Swiss-Prot
Match: A0LNN5 (L-lactate transporter OS=Syntrophobacter fumaroxidans (strain DSM 10017 / MPOB) OX=335543 GN=Sfum_3364 PE=1 SV=1)

HSP 1 Score: 47.8 bits (112), Expect = 5.0e-04
Identity = 36/141 (25.53%), Postives = 63/141 (44.68%), Query Frame = 0


Query: 361 FWLYFVAYFCGGTIGLVYSNNLGQISQSLGYSSLTSSLVTLYSSCSFFGRLISAGPDFMR 420
           FWL ++AYFCG   GL+   +L    +  G +++ ++     SS +F          +  
Sbjct: 230 FWLLYLAYFCGSFAGLMVIGHLAGFGRDAGLTAMAAA--GAVSSLAFSNAATRILSGWFV 289

Query: 421 EKVHFARTGWLALALVPNPIAFILLAASGSEIALQIGTGLIGLSSGFMFSASVSITSELF 480
           +K+   R  + AL  +       +    GS + L I   +IG + G MF+   +   + +
Sbjct: 290 DKIGI-RVYFAALFALQTAAMIAIFQLGGSVVGLSIVAIVIGWNYGAMFTLFPATCLQFY 349

Query: 481 GPNSSGVNHNILITNIPLGSF 502
           GP + G N+ +L T   L  F
Sbjct: 350 GPTAQGSNYGLLFTACGLAGF 367

[Close]

BLAST of CmaCh08G009830 vs. ExPASy TrEMBL
Match: A0A6J1KEQ9 (protein NUCLEAR FUSION DEFECTIVE 4-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111494334 PE=4 SV=1)

HSP 1 Score: 1138.6 bits (2944), Expect = 0.0e+00
Identity = 580/580 (100.00%), Postives = 580/580 (100.00%), Query Frame = 0


Query: 1   MKIKHRKPQNFPHLPVTKQRRMMNQPGKWVALMAAIWIQAFTGTNFDFPSYSSNLKAALG 60
           MKIKHRKPQNFPHLPVTKQRRMMNQPGKWVALMAAIWIQAFTGTNFDFPSYSSNLKAALG
Sbjct: 1   MKIKHRKPQNFPHLPVTKQRRMMNQPGKWVALMAAIWIQAFTGTNFDFPSYSSNLKAALG 60

Query: 61  MSQVELNYLAVASDVGKAFGWCSGIALLYFPSWVVMLMAASMGFLGYGLQWLLLQGIVSL 120
           MSQVELNYLAVASDVGKAFGWCSGIALLYFPSWVVMLMAASMGFLGYGLQWLLLQGIVSL
Sbjct: 61  MSQVELNYLAVASDVGKAFGWCSGIALLYFPSWVVMLMAASMGFLGYGLQWLLLQGIVSL 120

Query: 121 PYSMVYLLCLLAGCSICWFNTICYVSCIQKFPANRALALSLIVSFNGVSAALYTLMTNAV 180
           PYSMVYLLCLLAGCSICWFNTICYVSCIQKFPANRALALSLIVSFNGVSAALYTLMTNAV
Sbjct: 121 PYSMVYLLCLLAGCSICWFNTICYVSCIQKFPANRALALSLIVSFNGVSAALYTLMTNAV 180

Query: 181 DPNDASLYLLLNALVPLIISVVAFFPILHQPPAQPSSADATRNDSLTFICLYITAVITGL 240
           DPNDASLYLLLNALVPLIISVVAFFPILHQPPAQPSSADATRNDSLTFICLYITAVITGL
Sbjct: 181 DPNDASLYLLLNALVPLIISVVAFFPILHQPPAQPSSADATRNDSLTFICLYITAVITGL 240

Query: 241 YLITFNSTPSSKYGAQILLAGAFALLLVPLCLPGIWSTHKWLFRIASTGLSSVLHFRFNL 300
           YLITFNSTPSSKYGAQILLAGAFALLLVPLCLPGIWSTHKWLFRIASTGLSSVLHFRFNL
Sbjct: 241 YLITFNSTPSSKYGAQILLAGAFALLLVPLCLPGIWSTHKWLFRIASTGLSSVLHFRFNL 300

Query: 301 VNHELQQELINVESERSGTKVITPCDYKEKESISRKMMGKENLTVLEEEHSAKMLVRRLD 360
           VNHELQQELINVESERSGTKVITPCDYKEKESISRKMMGKENLTVLEEEHSAKMLVRRLD
Sbjct: 301 VNHELQQELINVESERSGTKVITPCDYKEKESISRKMMGKENLTVLEEEHSAKMLVRRLD 360

Query: 361 FWLYFVAYFCGGTIGLVYSNNLGQISQSLGYSSLTSSLVTLYSSCSFFGRLISAGPDFMR 420
           FWLYFVAYFCGGTIGLVYSNNLGQISQSLGYSSLTSSLVTLYSSCSFFGRLISAGPDFMR
Sbjct: 361 FWLYFVAYFCGGTIGLVYSNNLGQISQSLGYSSLTSSLVTLYSSCSFFGRLISAGPDFMR 420

Query: 421 EKVHFARTGWLALALVPNPIAFILLAASGSEIALQIGTGLIGLSSGFMFSASVSITSELF 480
           EKVHFARTGWLALALVPNPIAFILLAASGSEIALQIGTGLIGLSSGFMFSASVSITSELF
Sbjct: 421 EKVHFARTGWLALALVPNPIAFILLAASGSEIALQIGTGLIGLSSGFMFSASVSITSELF 480

Query: 481 GPNSSGVNHNILITNIPLGSFLYGVLAAVSYDSTAGSSNQTAATLGDVVVCIGRKCYLQT 540
           GPNSSGVNHNILITNIPLGSFLYGVLAAVSYDSTAGSSNQTAATLGDVVVCIGRKCYLQT
Sbjct: 481 GPNSSGVNHNILITNIPLGSFLYGVLAAVSYDSTAGSSNQTAATLGDVVVCIGRKCYLQT 540

Query: 541 FIWWACISIVGLASCFLLFRRTKPAYDRHYESNPSKTQPF 581
           FIWWACISIVGLASCFLLFRRTKPAYDRHYESNPSKTQPF
Sbjct: 541 FIWWACISIVGLASCFLLFRRTKPAYDRHYESNPSKTQPF 580

[Close]

BLAST of CmaCh08G009830 vs. ExPASy TrEMBL
Match: A0A6J1KH74 (protein NUCLEAR FUSION DEFECTIVE 4-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111494334 PE=4 SV=1)

HSP 1 Score: 1131.7 bits (2926), Expect = 0.0e+00
Identity = 580/587 (98.81%), Postives = 580/587 (98.81%), Query Frame = 0


Query: 1   MKIKHRKPQNFPHLPVTKQRRMMNQPGKWVALMAAIWIQAFTGTNFDFPSYSSNLKAALG 60
           MKIKHRKPQNFPHLPVTKQRRMMNQPGKWVALMAAIWIQAFTGTNFDFPSYSSNLKAALG
Sbjct: 1   MKIKHRKPQNFPHLPVTKQRRMMNQPGKWVALMAAIWIQAFTGTNFDFPSYSSNLKAALG 60

Query: 61  MSQVELNYLAVASDVGKAFGWCSGIALLYFPSWVVMLMAASMGFLGYGLQWLLLQGIVSL 120
           MSQVELNYLAVASDVGKAFGWCSGIALLYFPSWVVMLMAASMGFLGYGLQWLLLQGIVSL
Sbjct: 61  MSQVELNYLAVASDVGKAFGWCSGIALLYFPSWVVMLMAASMGFLGYGLQWLLLQGIVSL 120

Query: 121 PYSMVYLLCLLAGCSICWFNTICYVSCIQKFPANRALALSLIVSFNGVSAALYTLMTNAV 180
           PYSMVYLLCLLAGCSICWFNTICYVSCIQKFPANRALALSLIVSFNGVSAALYTLMTNAV
Sbjct: 121 PYSMVYLLCLLAGCSICWFNTICYVSCIQKFPANRALALSLIVSFNGVSAALYTLMTNAV 180

Query: 181 DPNDASLYLLLNALVPLIISVVAFFPILHQPPAQPSSADATRNDSLTFICLYITAVITGL 240
           DPNDASLYLLLNALVPLIISVVAFFPILHQPPAQPSSADATRNDSLTFICLYITAVITGL
Sbjct: 181 DPNDASLYLLLNALVPLIISVVAFFPILHQPPAQPSSADATRNDSLTFICLYITAVITGL 240

Query: 241 YLITFNSTPSSKYGAQILLAGAFALLLVPLCLPGIWSTHKWLFRIASTGLSSVLHFRFNL 300
           YLITFNSTPSSKYGAQILLAGAFALLLVPLCLPGIWSTHKWLFRIASTGLSSVLHFRFNL
Sbjct: 241 YLITFNSTPSSKYGAQILLAGAFALLLVPLCLPGIWSTHKWLFRIASTGLSSVLHFRFNL 300

Query: 301 VNHELQQELINVESERSGTKVITPCDYKEKESISRKMMGKENLTVLEEEHSAKMLVRRLD 360
           VNHELQQELINVESERSGTKVITPCDYKEKESISRKMMGKENLTVLEEEHSAKMLVRRLD
Sbjct: 301 VNHELQQELINVESERSGTKVITPCDYKEKESISRKMMGKENLTVLEEEHSAKMLVRRLD 360

Query: 361 FWLYFVAYFCGGTIGLVYSNNLGQISQSLGYSSLTSSLVTLYSSCSFFGRLISAGPDFMR 420
           FWLYFVAYFCGGTIGLVYSNNLGQISQSLGYSSLTSSLVTLYSSCSFFGRLISAGPDFMR
Sbjct: 361 FWLYFVAYFCGGTIGLVYSNNLGQISQSLGYSSLTSSLVTLYSSCSFFGRLISAGPDFMR 420

Query: 421 E-------KVHFARTGWLALALVPNPIAFILLAASGSEIALQIGTGLIGLSSGFMFSASV 480
           E       KVHFARTGWLALALVPNPIAFILLAASGSEIALQIGTGLIGLSSGFMFSASV
Sbjct: 421 EYVVAANGKVHFARTGWLALALVPNPIAFILLAASGSEIALQIGTGLIGLSSGFMFSASV 480

Query: 481 SITSELFGPNSSGVNHNILITNIPLGSFLYGVLAAVSYDSTAGSSNQTAATLGDVVVCIG 540
           SITSELFGPNSSGVNHNILITNIPLGSFLYGVLAAVSYDSTAGSSNQTAATLGDVVVCIG
Sbjct: 481 SITSELFGPNSSGVNHNILITNIPLGSFLYGVLAAVSYDSTAGSSNQTAATLGDVVVCIG 540

Query: 541 RKCYLQTFIWWACISIVGLASCFLLFRRTKPAYDRHYESNPSKTQPF 581
           RKCYLQTFIWWACISIVGLASCFLLFRRTKPAYDRHYESNPSKTQPF
Sbjct: 541 RKCYLQTFIWWACISIVGLASCFLLFRRTKPAYDRHYESNPSKTQPF 587

[Close]

BLAST of CmaCh08G009830 vs. ExPASy TrEMBL
Match: A0A6J1HJV4 (protein NUCLEAR FUSION DEFECTIVE 4-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111464237 PE=4 SV=1)

HSP 1 Score: 1097.0 bits (2836), Expect = 0.0e+00
Identity = 558/577 (96.71%), Postives = 566/577 (98.09%), Query Frame = 0


Query: 1   MKIKHRKPQNFPHLPVTKQRRMMNQPGKWVALMAAIWIQAFTGTNFDFPSYSSNLKAALG 60
           MKIKHRKPQN P LPVTKQRRMMNQPGKWVAL+AAIWIQAFTGTNFDFPSYSSNLKAALG
Sbjct: 1   MKIKHRKPQNLPLLPVTKQRRMMNQPGKWVALVAAIWIQAFTGTNFDFPSYSSNLKAALG 60

Query: 61  MSQVELNYLAVASDVGKAFGWCSGIALLYFPSWVVMLMAASMGFLGYGLQWLLLQGIVSL 120
           MSQVELNYLAVASDVGKAFGWCSGIALLYFP WVVMLMAASMGFLGYGLQWLLLQGIVSL
Sbjct: 61  MSQVELNYLAVASDVGKAFGWCSGIALLYFPLWVVMLMAASMGFLGYGLQWLLLQGIVSL 120

Query: 121 PYSMVYLLCLLAGCSICWFNTICYVSCIQKFPANRALALSLIVSFNGVSAALYTLMTNAV 180
           PYSMVYLLCLLAGCSICWFNTICYVSCIQKFP NRALALSLIVSFNGVSAALYTLM NA+
Sbjct: 121 PYSMVYLLCLLAGCSICWFNTICYVSCIQKFPTNRALALSLIVSFNGVSAALYTLMANAL 180

Query: 181 DPNDASLYLLLNALVPLIISVVAFFPILHQPPAQPSSADATRNDSLTFICLYITAVITGL 240
           DPNDASLYLLLNALVPLIISVV F PILHQPPAQPSSADATRNDSLTFICLYITAVITGL
Sbjct: 181 DPNDASLYLLLNALVPLIISVVTFLPILHQPPAQPSSADATRNDSLTFICLYITAVITGL 240

Query: 241 YLITFNSTPSSKYGAQILLAGAFALLLVPLCLPGIWSTHKWLFRIASTGLSSVLHFRFNL 300
           YLITFNSTPSSKYGAQ+LLAGAFALLLVPLCLPGIWSTHKWLFRIASTGLSSVLHFRFNL
Sbjct: 241 YLITFNSTPSSKYGAQLLLAGAFALLLVPLCLPGIWSTHKWLFRIASTGLSSVLHFRFNL 300

Query: 301 VNHELQQELINVESERSGTKVITPCDYKEKESISRKMMGKENLTVLEEEHSAKMLVRRLD 360
           VNHELQQELI +E+ERSGTKVITP DYKEKESISRKMMGKENLTVLEEEHSAKML+RRLD
Sbjct: 301 VNHELQQELIPIETERSGTKVITPFDYKEKESISRKMMGKENLTVLEEEHSAKMLIRRLD 360

Query: 361 FWLYFVAYFCGGTIGLVYSNNLGQISQSLGYSSLTSSLVTLYSSCSFFGRLISAGPDFMR 420
           FWLYFVAYFCGGTIGLVYSNNLGQISQSLGYSSLTSSLVTLYSSCSFFGRLISAGPDFMR
Sbjct: 361 FWLYFVAYFCGGTIGLVYSNNLGQISQSLGYSSLTSSLVTLYSSCSFFGRLISAGPDFMR 420

Query: 421 EKVHFARTGWLALALVPNPIAFILLAASGSEIALQIGTGLIGLSSGFMFSASVSITSELF 480
           EKVHFARTGWLALALVP PIAFILLAASGSEIALQIGTGLIGLSSGFMFSASVSITSELF
Sbjct: 421 EKVHFARTGWLALALVPTPIAFILLAASGSEIALQIGTGLIGLSSGFMFSASVSITSELF 480

Query: 481 GPNSSGVNHNILITNIPLGSFLYGVLAAVSYDSTAGSSNQTAATLGDVVVCIGRKCYLQT 540
           GPNSSGVNHNILITNIPLGSFLYGVLAAVSYDSTAGSSNQTAA LGDVVVCIGRKCYLQT
Sbjct: 481 GPNSSGVNHNILITNIPLGSFLYGVLAAVSYDSTAGSSNQTAAALGDVVVCIGRKCYLQT 540

Query: 541 FIWWACISIVGLASCFLLFRRTKPAYDRHYESNPSKT 578
           FIWWACISI+GLASCFLLFRRTKPAYDRHYESNP+KT
Sbjct: 541 FIWWACISIIGLASCFLLFRRTKPAYDRHYESNPNKT 577

[Close]

BLAST of CmaCh08G009830 vs. ExPASy TrEMBL
Match: A0A6J1HGX4 (protein NUCLEAR FUSION DEFECTIVE 4-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111464237 PE=4 SV=1)

HSP 1 Score: 1090.1 bits (2818), Expect = 0.0e+00
Identity = 558/584 (95.55%), Postives = 566/584 (96.92%), Query Frame = 0


Query: 1   MKIKHRKPQNFPHLPVTKQRRMMNQPGKWVALMAAIWIQAFTGTNFDFPSYSSNLKAALG 60
           MKIKHRKPQN P LPVTKQRRMMNQPGKWVAL+AAIWIQAFTGTNFDFPSYSSNLKAALG
Sbjct: 1   MKIKHRKPQNLPLLPVTKQRRMMNQPGKWVALVAAIWIQAFTGTNFDFPSYSSNLKAALG 60

Query: 61  MSQVELNYLAVASDVGKAFGWCSGIALLYFPSWVVMLMAASMGFLGYGLQWLLLQGIVSL 120
           MSQVELNYLAVASDVGKAFGWCSGIALLYFP WVVMLMAASMGFLGYGLQWLLLQGIVSL
Sbjct: 61  MSQVELNYLAVASDVGKAFGWCSGIALLYFPLWVVMLMAASMGFLGYGLQWLLLQGIVSL 120

Query: 121 PYSMVYLLCLLAGCSICWFNTICYVSCIQKFPANRALALSLIVSFNGVSAALYTLMTNAV 180
           PYSMVYLLCLLAGCSICWFNTICYVSCIQKFP NRALALSLIVSFNGVSAALYTLM NA+
Sbjct: 121 PYSMVYLLCLLAGCSICWFNTICYVSCIQKFPTNRALALSLIVSFNGVSAALYTLMANAL 180

Query: 181 DPNDASLYLLLNALVPLIISVVAFFPILHQPPAQPSSADATRNDSLTFICLYITAVITGL 240
           DPNDASLYLLLNALVPLIISVV F PILHQPPAQPSSADATRNDSLTFICLYITAVITGL
Sbjct: 181 DPNDASLYLLLNALVPLIISVVTFLPILHQPPAQPSSADATRNDSLTFICLYITAVITGL 240

Query: 241 YLITFNSTPSSKYGAQILLAGAFALLLVPLCLPGIWSTHKWLFRIASTGLSSVLHFRFNL 300
           YLITFNSTPSSKYGAQ+LLAGAFALLLVPLCLPGIWSTHKWLFRIASTGLSSVLHFRFNL
Sbjct: 241 YLITFNSTPSSKYGAQLLLAGAFALLLVPLCLPGIWSTHKWLFRIASTGLSSVLHFRFNL 300

Query: 301 VNHELQQELINVESERSGTKVITPCDYKEKESISRKMMGKENLTVLEEEHSAKMLVRRLD 360
           VNHELQQELI +E+ERSGTKVITP DYKEKESISRKMMGKENLTVLEEEHSAKML+RRLD
Sbjct: 301 VNHELQQELIPIETERSGTKVITPFDYKEKESISRKMMGKENLTVLEEEHSAKMLIRRLD 360

Query: 361 FWLYFVAYFCGGTIGLVYSNNLGQISQSLGYSSLTSSLVTLYSSCSFFGRLISAGPDFMR 420
           FWLYFVAYFCGGTIGLVYSNNLGQISQSLGYSSLTSSLVTLYSSCSFFGRLISAGPDFMR
Sbjct: 361 FWLYFVAYFCGGTIGLVYSNNLGQISQSLGYSSLTSSLVTLYSSCSFFGRLISAGPDFMR 420

Query: 421 E-------KVHFARTGWLALALVPNPIAFILLAASGSEIALQIGTGLIGLSSGFMFSASV 480
           E       KVHFARTGWLALALVP PIAFILLAASGSEIALQIGTGLIGLSSGFMFSASV
Sbjct: 421 EYVVATNGKVHFARTGWLALALVPTPIAFILLAASGSEIALQIGTGLIGLSSGFMFSASV 480

Query: 481 SITSELFGPNSSGVNHNILITNIPLGSFLYGVLAAVSYDSTAGSSNQTAATLGDVVVCIG 540
           SITSELFGPNSSGVNHNILITNIPLGSFLYGVLAAVSYDSTAGSSNQTAA LGDVVVCIG
Sbjct: 481 SITSELFGPNSSGVNHNILITNIPLGSFLYGVLAAVSYDSTAGSSNQTAAALGDVVVCIG 540

Query: 541 RKCYLQTFIWWACISIVGLASCFLLFRRTKPAYDRHYESNPSKT 578
           RKCYLQTFIWWACISI+GLASCFLLFRRTKPAYDRHYESNP+KT
Sbjct: 541 RKCYLQTFIWWACISIIGLASCFLLFRRTKPAYDRHYESNPNKT 584

[Close]

BLAST of CmaCh08G009830 vs. ExPASy TrEMBL
Match: A0A1S3CCZ1 (protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucumis melo OX=3656 GN=LOC103499000 PE=4 SV=1)

HSP 1 Score: 949.1 bits (2452), Expect = 8.5e-273
Identity = 484/580 (83.45%), Postives = 521/580 (89.83%), Query Frame = 0


Query: 1   MKIKHRKPQNFPHLPVTKQRRMMNQPGKWVALMAAIWIQAFTGTNFDFPSYSSNLKAALG 60
           MKIKHRK Q  P LP  KQ+RMMNQPGKWV L+AAIWIQAF GTNFDFPSYSS+LKAALG
Sbjct: 1   MKIKHRKTQQIPLLPFKKQKRMMNQPGKWVVLVAAIWIQAFAGTNFDFPSYSSDLKAALG 60

Query: 61  MSQVELNYLAVASDVGKAFGWCSGIALLYFPSWVVMLMAASMGFLGYGLQWLLLQGIVSL 120
           MSQVELNYLAVASD+GKAFGWCSG+ALLYFP WVVM MAASMGFLGYGLQWLLLQ I+SL
Sbjct: 61  MSQVELNYLAVASDLGKAFGWCSGVALLYFPLWVVMFMAASMGFLGYGLQWLLLQRIISL 120

Query: 121 PYSMVYLLCLLAGCSICWFNTICYVSCIQKFPANRALALSLIVSFNGVSAALYTLMTNAV 180
           PYS+VYLLCL+AGCSICWFNT+CYVSCIQ FPANRALALSLIVSFNGVSAALYTL+ NA+
Sbjct: 121 PYSVVYLLCLMAGCSICWFNTVCYVSCIQNFPANRALALSLIVSFNGVSAALYTLIANAI 180

Query: 181 DPNDASLYLLLNALVPLIISVVAFFPILHQPPAQPSSADATRNDSLTFICLYITAVITGL 240
           DP DASLYL LNALVPLIISVVA  P+LHQP  QPSSADA R+DSL FICLYITAVITGL
Sbjct: 181 DPGDASLYLFLNALVPLIISVVALLPMLHQPQVQPSSADAIRHDSLIFICLYITAVITGL 240

Query: 241 YLITFNSTPSSKYGAQILLAGAFALLLVPLCLPGIWSTHKWLFRIASTGLSSVLHFRFNL 300
           YLITFNS PS+KYG+QILLAGAFALLLVPLCLPGI STH+WL RI ST L+ ++H RF+L
Sbjct: 241 YLITFNSMPSNKYGSQILLAGAFALLLVPLCLPGILSTHRWLVRIISTSLNCLIHSRFSL 300

Query: 301 VNHELQQELINVESERSGTKVITPCDYKEKESISRKMMGKENLTVLEEEHSAKMLVRRLD 360
            +HEL QEL  + SER+    I P + KEKESISRK+M KENL VLEEEHSAKML+R+LD
Sbjct: 301 ADHELHQELFTIGSERNSMMDIIPFESKEKESISRKVMEKENLVVLEEEHSAKMLMRQLD 360

Query: 361 FWLYFVAYFCGGTIGLVYSNNLGQISQSLGYSSLTSSLVTLYSSCSFFGRLISAGPDFMR 420
           FWLY+ AYFCGGTIGLVYSNNLGQI+QSLGYSS TSSLVTLYSSCSFFGRLISA PDFMR
Sbjct: 361 FWLYYAAYFCGGTIGLVYSNNLGQIAQSLGYSSSTSSLVTLYSSCSFFGRLISAAPDFMR 420

Query: 421 EKVHFARTGWLALALVPNPIAFILLAASGSEIALQIGTGLIGLSSGFMFSASVSITSELF 480
           EK HFARTGWLA+ALVP PIAF+LLAASGS+IALQ GT LIGLSSGF+FSASVSITSELF
Sbjct: 421 EKGHFARTGWLAIALVPTPIAFVLLAASGSKIALQAGTSLIGLSSGFIFSASVSITSELF 480

Query: 481 GPNSSGVNHNILITNIPLGSFLYGVLAAVSYDSTAGSSNQTAATLGDVVVCIGRKCYLQT 540
           GPNSSGVNHNILITNIPLGSFLYGVLAAV+YDS AGSS+QTAA LGD VVCIG+ CYL T
Sbjct: 481 GPNSSGVNHNILITNIPLGSFLYGVLAAVAYDSNAGSSHQTAA-LGDAVVCIGQNCYLMT 540

Query: 541 FIWWACISIVGLASCFLLFRRTKPAYDRHYESNPSKTQPF 581
           F+WWACISI GLAS FLLFRRTK AYDRHYESN SK QPF
Sbjct: 541 FVWWACISIFGLASSFLLFRRTKSAYDRHYESNLSKMQPF 579

[Close]

BLAST of CmaCh08G009830 vs. NCBI nr
Match: XP_023000026.1 (protein NUCLEAR FUSION DEFECTIVE 4-like isoform X2 [Cucurbita maxima])

HSP 1 Score: 1138.6 bits (2944), Expect = 0.0e+00
Identity = 580/580 (100.00%), Postives = 580/580 (100.00%), Query Frame = 0


Query: 1   MKIKHRKPQNFPHLPVTKQRRMMNQPGKWVALMAAIWIQAFTGTNFDFPSYSSNLKAALG 60
           MKIKHRKPQNFPHLPVTKQRRMMNQPGKWVALMAAIWIQAFTGTNFDFPSYSSNLKAALG
Sbjct: 1   MKIKHRKPQNFPHLPVTKQRRMMNQPGKWVALMAAIWIQAFTGTNFDFPSYSSNLKAALG 60

Query: 61  MSQVELNYLAVASDVGKAFGWCSGIALLYFPSWVVMLMAASMGFLGYGLQWLLLQGIVSL 120
           MSQVELNYLAVASDVGKAFGWCSGIALLYFPSWVVMLMAASMGFLGYGLQWLLLQGIVSL
Sbjct: 61  MSQVELNYLAVASDVGKAFGWCSGIALLYFPSWVVMLMAASMGFLGYGLQWLLLQGIVSL 120

Query: 121 PYSMVYLLCLLAGCSICWFNTICYVSCIQKFPANRALALSLIVSFNGVSAALYTLMTNAV 180
           PYSMVYLLCLLAGCSICWFNTICYVSCIQKFPANRALALSLIVSFNGVSAALYTLMTNAV
Sbjct: 121 PYSMVYLLCLLAGCSICWFNTICYVSCIQKFPANRALALSLIVSFNGVSAALYTLMTNAV 180

Query: 181 DPNDASLYLLLNALVPLIISVVAFFPILHQPPAQPSSADATRNDSLTFICLYITAVITGL 240
           DPNDASLYLLLNALVPLIISVVAFFPILHQPPAQPSSADATRNDSLTFICLYITAVITGL
Sbjct: 181 DPNDASLYLLLNALVPLIISVVAFFPILHQPPAQPSSADATRNDSLTFICLYITAVITGL 240

Query: 241 YLITFNSTPSSKYGAQILLAGAFALLLVPLCLPGIWSTHKWLFRIASTGLSSVLHFRFNL 300
           YLITFNSTPSSKYGAQILLAGAFALLLVPLCLPGIWSTHKWLFRIASTGLSSVLHFRFNL
Sbjct: 241 YLITFNSTPSSKYGAQILLAGAFALLLVPLCLPGIWSTHKWLFRIASTGLSSVLHFRFNL 300

Query: 301 VNHELQQELINVESERSGTKVITPCDYKEKESISRKMMGKENLTVLEEEHSAKMLVRRLD 360
           VNHELQQELINVESERSGTKVITPCDYKEKESISRKMMGKENLTVLEEEHSAKMLVRRLD
Sbjct: 301 VNHELQQELINVESERSGTKVITPCDYKEKESISRKMMGKENLTVLEEEHSAKMLVRRLD 360

Query: 361 FWLYFVAYFCGGTIGLVYSNNLGQISQSLGYSSLTSSLVTLYSSCSFFGRLISAGPDFMR 420
           FWLYFVAYFCGGTIGLVYSNNLGQISQSLGYSSLTSSLVTLYSSCSFFGRLISAGPDFMR
Sbjct: 361 FWLYFVAYFCGGTIGLVYSNNLGQISQSLGYSSLTSSLVTLYSSCSFFGRLISAGPDFMR 420

Query: 421 EKVHFARTGWLALALVPNPIAFILLAASGSEIALQIGTGLIGLSSGFMFSASVSITSELF 480
           EKVHFARTGWLALALVPNPIAFILLAASGSEIALQIGTGLIGLSSGFMFSASVSITSELF
Sbjct: 421 EKVHFARTGWLALALVPNPIAFILLAASGSEIALQIGTGLIGLSSGFMFSASVSITSELF 480

Query: 481 GPNSSGVNHNILITNIPLGSFLYGVLAAVSYDSTAGSSNQTAATLGDVVVCIGRKCYLQT 540
           GPNSSGVNHNILITNIPLGSFLYGVLAAVSYDSTAGSSNQTAATLGDVVVCIGRKCYLQT
Sbjct: 481 GPNSSGVNHNILITNIPLGSFLYGVLAAVSYDSTAGSSNQTAATLGDVVVCIGRKCYLQT 540

Query: 541 FIWWACISIVGLASCFLLFRRTKPAYDRHYESNPSKTQPF 581
           FIWWACISIVGLASCFLLFRRTKPAYDRHYESNPSKTQPF
Sbjct: 541 FIWWACISIVGLASCFLLFRRTKPAYDRHYESNPSKTQPF 580

[Close]

BLAST of CmaCh08G009830 vs. NCBI nr
Match: XP_023000025.1 (protein NUCLEAR FUSION DEFECTIVE 4-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 1131.7 bits (2926), Expect = 0.0e+00
Identity = 580/587 (98.81%), Postives = 580/587 (98.81%), Query Frame = 0


Query: 1   MKIKHRKPQNFPHLPVTKQRRMMNQPGKWVALMAAIWIQAFTGTNFDFPSYSSNLKAALG 60
           MKIKHRKPQNFPHLPVTKQRRMMNQPGKWVALMAAIWIQAFTGTNFDFPSYSSNLKAALG
Sbjct: 1   MKIKHRKPQNFPHLPVTKQRRMMNQPGKWVALMAAIWIQAFTGTNFDFPSYSSNLKAALG 60

Query: 61  MSQVELNYLAVASDVGKAFGWCSGIALLYFPSWVVMLMAASMGFLGYGLQWLLLQGIVSL 120
           MSQVELNYLAVASDVGKAFGWCSGIALLYFPSWVVMLMAASMGFLGYGLQWLLLQGIVSL
Sbjct: 61  MSQVELNYLAVASDVGKAFGWCSGIALLYFPSWVVMLMAASMGFLGYGLQWLLLQGIVSL 120

Query: 121 PYSMVYLLCLLAGCSICWFNTICYVSCIQKFPANRALALSLIVSFNGVSAALYTLMTNAV 180
           PYSMVYLLCLLAGCSICWFNTICYVSCIQKFPANRALALSLIVSFNGVSAALYTLMTNAV
Sbjct: 121 PYSMVYLLCLLAGCSICWFNTICYVSCIQKFPANRALALSLIVSFNGVSAALYTLMTNAV 180

Query: 181 DPNDASLYLLLNALVPLIISVVAFFPILHQPPAQPSSADATRNDSLTFICLYITAVITGL 240
           DPNDASLYLLLNALVPLIISVVAFFPILHQPPAQPSSADATRNDSLTFICLYITAVITGL
Sbjct: 181 DPNDASLYLLLNALVPLIISVVAFFPILHQPPAQPSSADATRNDSLTFICLYITAVITGL 240

Query: 241 YLITFNSTPSSKYGAQILLAGAFALLLVPLCLPGIWSTHKWLFRIASTGLSSVLHFRFNL 300
           YLITFNSTPSSKYGAQILLAGAFALLLVPLCLPGIWSTHKWLFRIASTGLSSVLHFRFNL
Sbjct: 241 YLITFNSTPSSKYGAQILLAGAFALLLVPLCLPGIWSTHKWLFRIASTGLSSVLHFRFNL 300

Query: 301 VNHELQQELINVESERSGTKVITPCDYKEKESISRKMMGKENLTVLEEEHSAKMLVRRLD 360
           VNHELQQELINVESERSGTKVITPCDYKEKESISRKMMGKENLTVLEEEHSAKMLVRRLD
Sbjct: 301 VNHELQQELINVESERSGTKVITPCDYKEKESISRKMMGKENLTVLEEEHSAKMLVRRLD 360

Query: 361 FWLYFVAYFCGGTIGLVYSNNLGQISQSLGYSSLTSSLVTLYSSCSFFGRLISAGPDFMR 420
           FWLYFVAYFCGGTIGLVYSNNLGQISQSLGYSSLTSSLVTLYSSCSFFGRLISAGPDFMR
Sbjct: 361 FWLYFVAYFCGGTIGLVYSNNLGQISQSLGYSSLTSSLVTLYSSCSFFGRLISAGPDFMR 420

Query: 421 E-------KVHFARTGWLALALVPNPIAFILLAASGSEIALQIGTGLIGLSSGFMFSASV 480
           E       KVHFARTGWLALALVPNPIAFILLAASGSEIALQIGTGLIGLSSGFMFSASV
Sbjct: 421 EYVVAANGKVHFARTGWLALALVPNPIAFILLAASGSEIALQIGTGLIGLSSGFMFSASV 480

Query: 481 SITSELFGPNSSGVNHNILITNIPLGSFLYGVLAAVSYDSTAGSSNQTAATLGDVVVCIG 540
           SITSELFGPNSSGVNHNILITNIPLGSFLYGVLAAVSYDSTAGSSNQTAATLGDVVVCIG
Sbjct: 481 SITSELFGPNSSGVNHNILITNIPLGSFLYGVLAAVSYDSTAGSSNQTAATLGDVVVCIG 540

Query: 541 RKCYLQTFIWWACISIVGLASCFLLFRRTKPAYDRHYESNPSKTQPF 581
           RKCYLQTFIWWACISIVGLASCFLLFRRTKPAYDRHYESNPSKTQPF
Sbjct: 541 RKCYLQTFIWWACISIVGLASCFLLFRRTKPAYDRHYESNPSKTQPF 587

[Close]

BLAST of CmaCh08G009830 vs. NCBI nr
Match: XP_022964108.1 (protein NUCLEAR FUSION DEFECTIVE 4-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 1097.0 bits (2836), Expect = 0.0e+00
Identity = 558/577 (96.71%), Postives = 566/577 (98.09%), Query Frame = 0


Query: 1   MKIKHRKPQNFPHLPVTKQRRMMNQPGKWVALMAAIWIQAFTGTNFDFPSYSSNLKAALG 60
           MKIKHRKPQN P LPVTKQRRMMNQPGKWVAL+AAIWIQAFTGTNFDFPSYSSNLKAALG
Sbjct: 1   MKIKHRKPQNLPLLPVTKQRRMMNQPGKWVALVAAIWIQAFTGTNFDFPSYSSNLKAALG 60

Query: 61  MSQVELNYLAVASDVGKAFGWCSGIALLYFPSWVVMLMAASMGFLGYGLQWLLLQGIVSL 120
           MSQVELNYLAVASDVGKAFGWCSGIALLYFP WVVMLMAASMGFLGYGLQWLLLQGIVSL
Sbjct: 61  MSQVELNYLAVASDVGKAFGWCSGIALLYFPLWVVMLMAASMGFLGYGLQWLLLQGIVSL 120

Query: 121 PYSMVYLLCLLAGCSICWFNTICYVSCIQKFPANRALALSLIVSFNGVSAALYTLMTNAV 180
           PYSMVYLLCLLAGCSICWFNTICYVSCIQKFP NRALALSLIVSFNGVSAALYTLM NA+
Sbjct: 121 PYSMVYLLCLLAGCSICWFNTICYVSCIQKFPTNRALALSLIVSFNGVSAALYTLMANAL 180

Query: 181 DPNDASLYLLLNALVPLIISVVAFFPILHQPPAQPSSADATRNDSLTFICLYITAVITGL 240
           DPNDASLYLLLNALVPLIISVV F PILHQPPAQPSSADATRNDSLTFICLYITAVITGL
Sbjct: 181 DPNDASLYLLLNALVPLIISVVTFLPILHQPPAQPSSADATRNDSLTFICLYITAVITGL 240

Query: 241 YLITFNSTPSSKYGAQILLAGAFALLLVPLCLPGIWSTHKWLFRIASTGLSSVLHFRFNL 300
           YLITFNSTPSSKYGAQ+LLAGAFALLLVPLCLPGIWSTHKWLFRIASTGLSSVLHFRFNL
Sbjct: 241 YLITFNSTPSSKYGAQLLLAGAFALLLVPLCLPGIWSTHKWLFRIASTGLSSVLHFRFNL 300

Query: 301 VNHELQQELINVESERSGTKVITPCDYKEKESISRKMMGKENLTVLEEEHSAKMLVRRLD 360
           VNHELQQELI +E+ERSGTKVITP DYKEKESISRKMMGKENLTVLEEEHSAKML+RRLD
Sbjct: 301 VNHELQQELIPIETERSGTKVITPFDYKEKESISRKMMGKENLTVLEEEHSAKMLIRRLD 360

Query: 361 FWLYFVAYFCGGTIGLVYSNNLGQISQSLGYSSLTSSLVTLYSSCSFFGRLISAGPDFMR 420
           FWLYFVAYFCGGTIGLVYSNNLGQISQSLGYSSLTSSLVTLYSSCSFFGRLISAGPDFMR
Sbjct: 361 FWLYFVAYFCGGTIGLVYSNNLGQISQSLGYSSLTSSLVTLYSSCSFFGRLISAGPDFMR 420

Query: 421 EKVHFARTGWLALALVPNPIAFILLAASGSEIALQIGTGLIGLSSGFMFSASVSITSELF 480
           EKVHFARTGWLALALVP PIAFILLAASGSEIALQIGTGLIGLSSGFMFSASVSITSELF
Sbjct: 421 EKVHFARTGWLALALVPTPIAFILLAASGSEIALQIGTGLIGLSSGFMFSASVSITSELF 480

Query: 481 GPNSSGVNHNILITNIPLGSFLYGVLAAVSYDSTAGSSNQTAATLGDVVVCIGRKCYLQT 540
           GPNSSGVNHNILITNIPLGSFLYGVLAAVSYDSTAGSSNQTAA LGDVVVCIGRKCYLQT
Sbjct: 481 GPNSSGVNHNILITNIPLGSFLYGVLAAVSYDSTAGSSNQTAAALGDVVVCIGRKCYLQT 540

Query: 541 FIWWACISIVGLASCFLLFRRTKPAYDRHYESNPSKT 578
           FIWWACISI+GLASCFLLFRRTKPAYDRHYESNP+KT
Sbjct: 541 FIWWACISIIGLASCFLLFRRTKPAYDRHYESNPNKT 577

[Close]

BLAST of CmaCh08G009830 vs. NCBI nr
Match: XP_023515123.1 (protein NUCLEAR FUSION DEFECTIVE 4-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1096.3 bits (2834), Expect = 0.0e+00
Identity = 561/580 (96.72%), Postives = 567/580 (97.76%), Query Frame = 0


Query: 1   MKIKHRKPQNFPHLPVTKQRRMMNQPGKWVALMAAIWIQAFTGTNFDFPSYSSNLKAALG 60
           MKIKHRKPQN P LPVTKQRRMMNQPGKWVAL+AAIWIQAFTGTNFDFPSYSSNLKAALG
Sbjct: 1   MKIKHRKPQNLPLLPVTKQRRMMNQPGKWVALVAAIWIQAFTGTNFDFPSYSSNLKAALG 60

Query: 61  MSQVELNYLAVASDVGKAFGWCSGIALLYFPSWVVMLMAASMGFLGYGLQWLLLQGIVSL 120
           MSQVELNYLAVASDVGKAFGWCSGIALLYFP WVVMLMAASMGFLGYGLQWLLLQGIVSL
Sbjct: 61  MSQVELNYLAVASDVGKAFGWCSGIALLYFPLWVVMLMAASMGFLGYGLQWLLLQGIVSL 120

Query: 121 PYSMVYLLCLLAGCSICWFNTICYVSCIQKFPANRALALSLIVSFNGVSAALYTLMTNAV 180
           PYSMVYLLCLLAGCSICWFNTICYVSCIQKFPANRALALSLIVSFNGVSAALYTLM NAV
Sbjct: 121 PYSMVYLLCLLAGCSICWFNTICYVSCIQKFPANRALALSLIVSFNGVSAALYTLMANAV 180

Query: 181 DPNDASLYLLLNALVPLIISVVAFFPILHQPPAQPSSADATRNDSLTFICLYITAVITGL 240
           DPNDASLYLLLNALVPLIISVV F PILHQPPAQPSS+DATRNDS TFICLYITAVITGL
Sbjct: 181 DPNDASLYLLLNALVPLIISVVTFLPILHQPPAQPSSSDATRNDSHTFICLYITAVITGL 240

Query: 241 YLITFNSTPSSKYGAQILLAGAFALLLVPLCLPGIWSTHKWLFRIASTGLSSVLHFRFNL 300
           YLITFNSTPSSKYGAQ+LLAGAFALLLVPLCLPGIWSTHKWLFRIASTGLSSVLHFRFNL
Sbjct: 241 YLITFNSTPSSKYGAQLLLAGAFALLLVPLCLPGIWSTHKWLFRIASTGLSSVLHFRFNL 300

Query: 301 VNHELQQELINVESERSGTKVITPCDYKEKESISRKMMGKENLTVLEEEHSAKMLVRRLD 360
           VNHELQQELIN+E+ERSGT VITP DYKEKESISRKMMGKENLTVLEEEHSAKMLVRRLD
Sbjct: 301 VNHELQQELINLETERSGTNVITPFDYKEKESISRKMMGKENLTVLEEEHSAKMLVRRLD 360

Query: 361 FWLYFVAYFCGGTIGLVYSNNLGQISQSLGYSSLTSSLVTLYSSCSFFGRLISAGPDFMR 420
           FWLYFVAYFCGGTIGLVYSNNLGQISQSLGYSSLTSSLVTLYSSCSFFGRLISAGPDFMR
Sbjct: 361 FWLYFVAYFCGGTIGLVYSNNLGQISQSLGYSSLTSSLVTLYSSCSFFGRLISAGPDFMR 420

Query: 421 EKVHFARTGWLALALVPNPIAFILLAASGSEIALQIGTGLIGLSSGFMFSASVSITSELF 480
           EKVHFARTGWLALALVP PIAFILLAASGSEIALQIGTGLIGLSSGFMFSASVSITSELF
Sbjct: 421 EKVHFARTGWLALALVPTPIAFILLAASGSEIALQIGTGLIGLSSGFMFSASVSITSELF 480

Query: 481 GPNSSGVNHNILITNIPLGSFLYGVLAAVSYDSTAGSSNQTAATLGDVVVCIGRKCYLQT 540
           GPNSSGVNHNILITNIPLGSFLYGVLAAVSYDSTAGSS+QTAA LGDVVVCIGRKCYLQT
Sbjct: 481 GPNSSGVNHNILITNIPLGSFLYGVLAAVSYDSTAGSSDQTAAALGDVVVCIGRKCYLQT 540

Query: 541 FIWWACISIVGLASCFLLFRRTKPAYDRHYESNPSKTQPF 581
           FIWWACISIVGLAS FLLFRRTKPAYDR YESNPSKTQPF
Sbjct: 541 FIWWACISIVGLASSFLLFRRTKPAYDRQYESNPSKTQPF 580

[Close]

BLAST of CmaCh08G009830 vs. NCBI nr
Match: KAG7026076.1 (Protein NUCLEAR FUSION DEFECTIVE 4 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1092.0 bits (2823), Expect = 0.0e+00
Identity = 555/577 (96.19%), Postives = 565/577 (97.92%), Query Frame = 0


Query: 1   MKIKHRKPQNFPHLPVTKQRRMMNQPGKWVALMAAIWIQAFTGTNFDFPSYSSNLKAALG 60
           MKIKHRKPQN P LPVTKQRRMMNQPGKWVAL+ AIWIQAFTGTNFDFPSYSSNLKAALG
Sbjct: 1   MKIKHRKPQNLPLLPVTKQRRMMNQPGKWVALVVAIWIQAFTGTNFDFPSYSSNLKAALG 60

Query: 61  MSQVELNYLAVASDVGKAFGWCSGIALLYFPSWVVMLMAASMGFLGYGLQWLLLQGIVSL 120
           MSQVELNYLAVASDVGKAFGWCSGIALLYFP WVVMLMAASMGFLGYGLQWLLLQGIVSL
Sbjct: 61  MSQVELNYLAVASDVGKAFGWCSGIALLYFPLWVVMLMAASMGFLGYGLQWLLLQGIVSL 120

Query: 121 PYSMVYLLCLLAGCSICWFNTICYVSCIQKFPANRALALSLIVSFNGVSAALYTLMTNAV 180
           PYSMVYLLCLLAGCSICWFNTICYVSCIQKFP NRALALSLIVSFNGVSAALYTLM NA+
Sbjct: 121 PYSMVYLLCLLAGCSICWFNTICYVSCIQKFPTNRALALSLIVSFNGVSAALYTLMANAL 180

Query: 181 DPNDASLYLLLNALVPLIISVVAFFPILHQPPAQPSSADATRNDSLTFICLYITAVITGL 240
           DPNDASLYLLLNALVPLIISVV FFPILHQPPAQPSSADATRNDSLTFICLYITAV+TGL
Sbjct: 181 DPNDASLYLLLNALVPLIISVVTFFPILHQPPAQPSSADATRNDSLTFICLYITAVVTGL 240

Query: 241 YLITFNSTPSSKYGAQILLAGAFALLLVPLCLPGIWSTHKWLFRIASTGLSSVLHFRFNL 300
           YLITFNSTPSSKYGAQ+LLAGAF+LLLVPLCLPGIWSTHKWLFRIASTGLSSVLHFRFNL
Sbjct: 241 YLITFNSTPSSKYGAQLLLAGAFSLLLVPLCLPGIWSTHKWLFRIASTGLSSVLHFRFNL 300

Query: 301 VNHELQQELINVESERSGTKVITPCDYKEKESISRKMMGKENLTVLEEEHSAKMLVRRLD 360
           VNHELQQELI +E+ERSGTKVITP DYKEKESIS KMMGKENLTVLEEEHSAKMLVRRL+
Sbjct: 301 VNHELQQELIPIETERSGTKVITPFDYKEKESISWKMMGKENLTVLEEEHSAKMLVRRLE 360

Query: 361 FWLYFVAYFCGGTIGLVYSNNLGQISQSLGYSSLTSSLVTLYSSCSFFGRLISAGPDFMR 420
           FWLYFVAYFCGGTIGLVYSNNLGQISQSLGYSSLTSSLVTLYSSCSFFGRLISAGPDFMR
Sbjct: 361 FWLYFVAYFCGGTIGLVYSNNLGQISQSLGYSSLTSSLVTLYSSCSFFGRLISAGPDFMR 420

Query: 421 EKVHFARTGWLALALVPNPIAFILLAASGSEIALQIGTGLIGLSSGFMFSASVSITSELF 480
           EKVHFARTGWLALALVP PIAFILLAASGSEIALQIGTGLIGLSSGFMFSASVSITSELF
Sbjct: 421 EKVHFARTGWLALALVPTPIAFILLAASGSEIALQIGTGLIGLSSGFMFSASVSITSELF 480

Query: 481 GPNSSGVNHNILITNIPLGSFLYGVLAAVSYDSTAGSSNQTAATLGDVVVCIGRKCYLQT 540
           GPNSSGVNHNILITNIPLGSFLYGVLAAVSYDSTAGSSNQTAA LGDVVVCIGRKCYLQT
Sbjct: 481 GPNSSGVNHNILITNIPLGSFLYGVLAAVSYDSTAGSSNQTAAALGDVVVCIGRKCYLQT 540

Query: 541 FIWWACISIVGLASCFLLFRRTKPAYDRHYESNPSKT 578
           FIWWACISI+GLASCFLLFRRTKPAYDRHYESNP+KT
Sbjct: 541 FIWWACISIIGLASCFLLFRRTKPAYDRHYESNPNKT 577

[Close]

BLAST of CmaCh08G009830 vs. TAIR 10
Match: AT5G45275.1 (Major facilitator superfamily protein )

HSP 1 Score: 614.0 bits (1582), Expect = 1.2e-175
Identity = 326/563 (57.90%), Postives = 405/563 (71.94%), Query Frame = 0


Query: 22  MMNQPGKWVALMAAIWIQAFTGTNFDFPSYSSNLKAALGMSQVELNYLAVASDVGKAFGW 81
           M  Q  KW+ L+A IWIQAFTGTNFDF +YSSNLK+ LG+SQV+LNYLAVASD+GK FGW
Sbjct: 1   MAGQSRKWMILVATIWIQAFTGTNFDFSTYSSNLKSVLGISQVQLNYLAVASDLGKVFGW 60

Query: 82  CSGIALLYFPSWVVMLMAASMGFLGYGLQWLLLQGIVSLPYSMVYLLCLLAGCSICWFNT 141
            SG+ALLYFP W V+  AA MGF+GYG+QWL++  ++SLPY +V+L CLLAG SICWFNT
Sbjct: 61  SSGLALLYFPLWTVLFAAAIMGFVGYGVQWLVITNVISLPYILVFLCCLLAGLSICWFNT 120

Query: 142 ICYVSCIQKFPANRALALSLIVSFNGVSAALYTLMTNAVDPNDASLYLLLNALVPLIISV 201
           +C+V CI+ FPANR+LALSL VSFNGVSAALYTL  NA++P    LYLLLNALVPL +S 
Sbjct: 121 VCFVLCIRNFPANRSLALSLTVSFNGVSAALYTLAYNAINPVSTELYLLLNALVPLFVSF 180

Query: 202 VAFFPILHQPPAQPSSADATRNDSLTFICLYITAVITGLYLITFNSTPSSKYGAQILLAG 261
            A  PIL QPP +P   D  R DSL F+ L I AV+ G+YL+ F S  S    A++L  G
Sbjct: 181 AALIPILRQPPLEPLPPDGVRRDSLMFLLLNILAVLNGVYLLLFRSKTSDVTSARLLFGG 240

Query: 262 AFALLLVPLCLPGIWSTHKWL-------FRIASTGLSSV----LHFRFNLVNHELQQELI 321
           +  LL++PLCLPG+     W        FR+  +G   V    L     +V  E   E  
Sbjct: 241 SILLLILPLCLPGLVYARNWYLHNIHSSFRLEGSGFILVDVDELEMHKGMVTREASLEGY 300

Query: 322 NVESERSGTKVITPCDYK-----EKESISRKMMGKENLTVLEEEHSAKMLVRRLDFWLYF 381
            + ++     V TP D K     +      K++ +  L +L EEH    L+ R DFWLY+
Sbjct: 301 QLLNDDVVRAVNTP-DQKSFIEDDDGCCCTKVITRNQLGMLGEEHPLSFLLCRSDFWLYY 360

Query: 382 VAYFCGGTIGLVYSNNLGQISQSLGYSSLTSSLVTLYSSCSFFGRLISAGPDFMREKVHF 441
           +AYFCGGTIGLVYSNNLGQI+QSLG SS T++LVTLYSS SFFGRL+SA PD++R KV+F
Sbjct: 361 IAYFCGGTIGLVYSNNLGQIAQSLGQSSETTTLVTLYSSFSFFGRLLSATPDYIRAKVYF 420

Query: 442 ARTGWLALALVPNPIAFILLAASGSEIALQIGTGLIGLSSGFMFSASVSITSELFGPNSS 501
           ARTGWLA+AL+P  IA  LLA+SGS  ALQ GT LIGLSSGF+F+A+VSITSELFGPNS 
Sbjct: 421 ARTGWLAVALLPTTIALFLLASSGSLAALQAGTALIGLSSGFIFAAAVSITSELFGPNSV 480

Query: 502 GVNHNILITNIPLGSFLYGVLAAVSYDSTAGSSNQTAATLGDVVVCIGRKCYLQTFIWWA 561
           GVNHNILITNIP+GS +YG LAA+ Y+S + + ++T     + V+C+GR CYLQTF+WW 
Sbjct: 481 GVNHNILITNIPIGSLVYGFLAALVYESHSVAGSKT-----ESVICMGRDCYLQTFMWWG 540

Query: 562 CISIVGLASCFLLFRRTKPAYDR 569
           C+S++GLAS  +LF RT+ AY R
Sbjct: 541 CLSVIGLASSVVLFLRTRRAYQR 557

[Close]

BLAST of CmaCh08G009830 vs. TAIR 10
Match: AT4G19450.1 (Major facilitator superfamily protein )

HSP 1 Score: 612.1 bits (1577), Expect = 4.7e-175
Identity = 319/568 (56.16%), Postives = 405/568 (71.30%), Query Frame = 0


Query: 22  MMNQPGKWVALMAAIWIQAFTGTNFDFPSYSSNLKAALGMSQVELNYLAVASDVGKAFGW 81
           M  Q  KW+ L+A IWIQAFTGTNFDF +YSS+LK+ LG+SQV+LNYLAVASD+GK FGW
Sbjct: 1   MAGQSRKWMILVATIWIQAFTGTNFDFSAYSSDLKSVLGISQVQLNYLAVASDLGKVFGW 60

Query: 82  CSGIALLYFPSWVVMLMAASMGFLGYGLQWLLLQGIVSLPYSMVYLLCLLAGCSICWFNT 141
            SG+AL+YFP W V+  AA MGF+GYG+QWL++   +SLPY MV+L CLLAG SICWFNT
Sbjct: 61  SSGLALMYFPLWTVLFAAAFMGFVGYGVQWLVITHFISLPYIMVFLCCLLAGLSICWFNT 120

Query: 142 ICYVSCIQKFPANRALALSLIVSFNGVSAALYTLMTNAVDPNDASLYLLLNALVPLIISV 201
           +C+V CI  FPANR+LALSL VSFNGVSAALYTL  NA++P    LYLLLNAL+PLI+S 
Sbjct: 121 VCFVLCISNFPANRSLALSLTVSFNGVSAALYTLAYNAINPTSPELYLLLNALIPLIVSF 180

Query: 202 VAFFPILHQPPAQPSSADATRNDSLTFICLYITAVITGLYLITFNSTPSSKYGAQILLAG 261
            A  PIL QPP +P   D  R DSL F+ L I A + G+YL+ F S  S    A++L  G
Sbjct: 181 TAIIPILRQPPFEPLPPDGVRRDSLMFLLLNILAALNGVYLLLFGSNSSDLTSARLLFGG 240

Query: 262 AFALLLVPLCLPGIWSTHKWL-------FRIASTGLSSV----LHFRFNLVNHELQQE-- 321
           A  LL+ PLC+PG+     W        FR+  +G   V    L     ++ HE  +E  
Sbjct: 241 AILLLVFPLCIPGLVIARNWYNRTIHTSFRLEGSGFILVDPDELELHKGMLAHEANREGY 300

Query: 322 -LINVESERSGTKVITPCDYKEKESISRKMMGKENLTVLEEEHSAKMLVRRLDFWLYFVA 381
            L++ +  ++  K +   +    ES  +K++ ++ L  L  EHS  +L+ R DFWLY++ 
Sbjct: 301 QLLSDDVVQNPVKSVAVEEEDSDESCCKKLITRDQLEGLGIEHSLSLLLTRSDFWLYYIT 360

Query: 382 YFCGGTIGLVYSNNLGQISQSLGYSSLTSSLVTLYSSCSFFGRLISAGPDFMREKVHFAR 441
           YFCGGTIGLVYSNNLGQI+QSLG SS T++LVTLYS+ SFFGRL+SA PD++R KV+FAR
Sbjct: 361 YFCGGTIGLVYSNNLGQIAQSLGQSSNTTTLVTLYSAFSFFGRLLSATPDYIRAKVYFAR 420

Query: 442 TGWLALALVPNPIAFILLAASGSEIALQIGTGLIGLSSGFMFSASVSITSELFGPNSSGV 501
           TGWLA+AL+P P A  LLA+SG+  ALQ GT L+GLSSGF+F+A+VSITSELFGPNS GV
Sbjct: 421 TGWLAIALLPTPFALFLLASSGTASALQAGTALMGLSSGFIFAAAVSITSELFGPNSVGV 480

Query: 502 NHNILITNIPLGSFLYGVLAAVSYDSTAGSSNQTAATLGDVVVCIGRKCYLQTFIWWACI 561
           NHNILITNIP+GS +YG LAA+ YDS   +   T +   + VVC+GR CY  TF+WW C+
Sbjct: 481 NHNILITNIPIGSLIYGFLAALVYDSHGFTG--TKSMTSESVVCMGRDCYYLTFVWWGCL 540

Query: 562 SIVGLASCFLLFRRTKPAYDRHYESNPS 576
           S++GL S  +LF RT+ AY R  ++  S
Sbjct: 541 SLLGLGSSLVLFIRTRRAYQRFEQARIS 566

[Close]

BLAST of CmaCh08G009830 vs. TAIR 10
Match: AT1G31470.1 (Major facilitator superfamily protein )

HSP 1 Score: 552.4 bits (1422), Expect = 4.4e-157
Identity = 311/581 (53.53%), Postives = 401/581 (69.02%), Query Frame = 0


Query: 5   HRKPQNFPHLPVTKQRRMMNQPGKWVALMAAIWIQAFTGTNFDFPSYSSNLKAALGMSQV 64
           HRK     HLP    R M  +  KW  L+AAIWIQA TGTNFDF +YSS+LK+ LG+SQV
Sbjct: 27  HRK-----HLP--PLRTMFGRWRKWTVLVAAIWIQASTGTNFDFSAYSSHLKSVLGISQV 86

Query: 65  ELNYLAVASDVGKAFGWCSGIALLYFPSWVVMLMAASMGFLGYGLQWLLLQGIVSLPYSM 124
            LNYLAVASD+GKAFGW SGIAL YFP  VV+  AA+MGF+GYG+QWL++  I++LPYS+
Sbjct: 87  RLNYLAVASDLGKAFGWSSGIALGYFPLSVVLFAAAAMGFVGYGVQWLVITNIITLPYSL 146

Query: 125 VYLLCLLAGCSICWFNTICYVSCIQKFPANRALALSLIVSFNGVSAALYTLMTNAVDPND 184
           V+L CLLAG SICWFNT C++ CI+ FP NRALALSL VSFNG+SAALY+L  NA++P+ 
Sbjct: 147 VFLCCLLAGLSICWFNTACFILCIRHFPNNRALALSLTVSFNGISAALYSLAFNAINPSS 206

Query: 185 ASLYLLLNALVPLIISVVAFFPILHQPPAQPS-SADATRNDSLTFICLYITAVITGLYLI 244
           ++LYLLLN+LVPL++S  A +P+L +P    +   D+ R+DS  F  L + AVIT  +L+
Sbjct: 207 SNLYLLLNSLVPLVVSFAALYPVLTKPSLDTTPDYDSRRHDSHVFTILNVLAVITSFHLL 266

Query: 245 TFNSTPSSKYGAQILLAGAFALLLVPLCLPGIWSTHKWLFRIASTGLSSVLHFRFNLVNH 304
             +S+ SS   A++   GA  LL+ PLC P +     +   + +  L           NH
Sbjct: 267 LSSSSTSS---ARLNFIGAVVLLVFPLCAPLLVYARDYFLPVINARL-----------NH 326

Query: 305 ELQ-QELINV-ESERSGTKVITPCDYKEKESISRKMMGKENLTV-LEEEHSAKMLVRRLD 364
           E     ++N+ E +   T V +   Y+   +       KE  TV L +EHS ++L+ RL+
Sbjct: 327 ESSGYVMLNIDELKNQKTSVSSKTGYEHMGT------AKEGNTVRLGDEHSFRLLISRLE 386

Query: 365 FWLYFVAYFCGGTIGLVYSNNLGQISQSLGYSSLTSSLVTLYSSCSFFGRLISAGPDFMR 424
           FWLY++AYFCGGTIGLVYSNNLGQI+QSLG +S T  LVT+YSS SFFGRL+SA PDFM 
Sbjct: 387 FWLYYIAYFCGGTIGLVYSNNLGQIAQSLGQNSTT--LVTIYSSFSFFGRLLSAAPDFMH 446

Query: 425 EKVHFARTGWLALALVPNPIAFILLAASGS-EIALQIGTGLIGLSSGFMFSASVSITSEL 484
           ++    RTGW A+AL+P PIAF LLA S S + ALQ  T LIGLSSGF+F+A+VSITS+L
Sbjct: 447 KRFRLTRTGWFAIALLPTPIAFFLLAVSSSQQTALQTATALIGLSSGFIFAAAVSITSDL 506

Query: 485 FGPNSSGVNHNILITNIPLGSFLYGVLAAVSYDSTAGSSNQTAATLGDVVVCIGRKCYLQ 544
           FGPNS GVNHNILITNIP+GS LYG +AA  Y+  A +S      + D +VCIGR CY +
Sbjct: 507 FGPNSVGVNHNILITNIPIGSLLYGYIAASIYE--ANASPDITPIVSDSIVCIGRDCYFK 566

Query: 545 TFIWWACISIVGLASCFLLFRRTKPAYDRHYESNPSKTQPF 581
           TF++W C+SI+G+ S   L+ RTKP Y R  +   S T  +
Sbjct: 567 TFVFWGCLSILGVVSSLSLYIRTKPVYHRLEQDKVSLTSSY 576

[Close]

BLAST of CmaCh08G009830 vs. TAIR 10
Match: AT3G01630.1 (Major facilitator superfamily protein )

HSP 1 Score: 485.7 bits (1249), Expect = 5.1e-137
Identity = 272/571 (47.64%), Postives = 373/571 (65.32%), Query Frame = 0


Query: 6   RKPQNFPHLPVTKQRRMMNQPGKWVALMAAIWIQAFTGTNFDFPSYSSNLKAALGMSQVE 65
           ++P + P  P  +   M+    +W  L+AAIWIQAFTGTNFDF +YSS++K+++G+SQ  
Sbjct: 3   KQPPSPPKPP--RVNSMLQWWRRWTVLVAAIWIQAFTGTNFDFSAYSSDMKSSMGVSQSR 62

Query: 66  LNYLAVASDVGKAFGWCSGIALLYFPSWVVMLMAASMGFLGYGLQWLLLQGIVSLPYSMV 125
           LNY+AVASD+GKA GW SG A+ YFP   V+  AA+MG +GYG+QWL +  ++ LPYS+V
Sbjct: 63  LNYMAVASDLGKALGWSSGFAIAYFPVSGVLFAAAAMGLVGYGVQWLSIADVIDLPYSLV 122

Query: 126 YLLCLLAGCSICWFNTICYVSCIQKFPANRALALSLIVSFNGVSAALYTLMTNAVDPNDA 185
            + C LAG SICWFNT+ ++ CI+ F AN +LALSL+VSFNG+SAALYTL   A+    +
Sbjct: 123 LVCCSLAGLSICWFNTVSFILCIRHFKANHSLALSLVVSFNGISAALYTLGHEAISGKSS 182

Query: 186 S---LYLLLNALVPLIISVVAFFPILHQPPAQPSSADATRNDSLTFICLYITAVITGLYL 245
           +   +YLLLN+L+PLI+SV+A +P+L  P +  +    T +++  F+   + A++T  YL
Sbjct: 183 ASSDIYLLLNSLIPLIVSVLALWPVLTNPNSSETDTTRTHDETRIFVVFNVLALVTCFYL 242

Query: 246 ITFNSTPSSKYGAQILLAGAFALLLVPLCLPGIWSTHKWLFRIASTGLSSVLHFRFNLVN 305
           +  +S        +    GA  LLL PLC+P +   H+         L S  H      +
Sbjct: 243 LLPSSGTYLASSPRWHFLGAIFLLLFPLCVPFLDYIHR--------ALESCFH------H 302

Query: 306 HELQQELINVESERSGTKVITPCDYKEKESISRKMMGKENLTVLEEEHSAKMLVRRLDFW 365
           H      +N+E E    K+ +     E+ES         +   L +EHS  MLVR+L+FW
Sbjct: 303 HSSGYAAVNIE-EPKILKIKSQKSNAEEES---------DQVRLGDEHSLGMLVRKLEFW 362

Query: 366 LYFVAYFCGGTIGLVYSNNLGQISQSLGYSSLTS-SLVTLYSSCSFFGRLISAGPDFMRE 425
           LY+VAYFCGGTIGLVYSNNLGQI+QSLG SS  + SLVTL+S+ SF GRL+S+ PDF R+
Sbjct: 363 LYYVAYFCGGTIGLVYSNNLGQIAQSLGQSSSNAKSLVTLFSAFSFLGRLLSSAPDFTRK 422

Query: 426 KV-HFARTGWLALALVPNPIAFILLAAS---GSEIALQIGTGLIGLSSGFMFSASVSITS 485
           K+ +  RTGW  ++L+P P+AF +LA S        L++ T LIGLSSGF+F+A+VSITS
Sbjct: 423 KLKYLTRTGWFTISLLPTPLAFFILAYSPKTNQTALLEVATALIGLSSGFVFAAAVSITS 482

Query: 486 ELFGPNSSGVNHNILITNIPLGSFLYGVLAAVSYDSTAGSSNQTAATLGDVVVCIGRKCY 545
           ELFG NS GVN NILITNIP+GS  YG +A   YD+ A    +  + + D VVC+GRKCY
Sbjct: 483 ELFGRNSVGVNQNILITNIPIGSLFYGYMAGSVYDTNASLGRK--SVVADSVVCVGRKCY 542

Query: 546 LQTFIWWACISIVGLASCFLLFRRTKPAYDR 569
             TF++W C+S++G      LF RT+P Y R
Sbjct: 543 FVTFLFWGCLSVLGFVCSLFLFIRTRPVYHR 545

[Close]

BLAST of CmaCh08G009830 vs. TAIR 10
Match: AT2G30300.1 (Major facilitator superfamily protein )

HSP 1 Score: 290.8 bits (743), Expect = 2.4e-78
Identity = 202/542 (37.27%), Postives = 298/542 (54.98%), Query Frame = 0


Query: 29  WVALMAAIWIQAFTGTNFDFPSYSSNLKAALGMSQVELNYLAVASDVGKAFGWCSGIALL 88
           W++L+A +W+Q+F GTN  FP+YSS LK  L +SQ +LNYL+ ASD GK  G+ SGIA +
Sbjct: 8   WLSLVAILWLQSFNGTNLSFPAYSSQLKELLKISQFKLNYLSFASDAGKVLGFISGIAAV 67

Query: 89  YFPSWVVMLMAASMGFLGYGLQWLLLQGIVSLPYSMVYLLCLLAGCSICWFNTICYVSCI 148
           Y P  +V+L   S+GF GYGLQ+L          S++  +       ICW NT CY+  I
Sbjct: 68  YLPLPLVLLAGGSLGFAGYGLQYL----------SIIKKI-------ICWINTACYIVAI 127

Query: 149 QKFPANRALALSLIVSFNGVSAALYTLMTNAV----DPNDASLYLLLNALVPLIISVVAF 208
             FP NR +A+ +  S+ G+S  +YT M ++        +AS YLLLN+LVPL+  +V  
Sbjct: 128 NSFPVNRQVAVGITASYQGLSGKIYTDMVHSFFHTSQREEASGYLLLNSLVPLVACLVT- 187

Query: 209 FPILHQPPAQPSSADATRNDSLTFICLYITAVITGLYLITFNSTPSSKYGAQILLAGAFA 268
            P+L +      +   +++  + FI L++  + TG+Y +   +T      A ++L G   
Sbjct: 188 APMLMRHGGD-KTMSYSKDVKVGFIVLFVLTIATGIYAV---ATSLVSVPAVLVLVGIAL 247

Query: 269 LLLVPLCLPGIWSTHKWLFRIASTGLSSVLHFRFNLVNHELQQELINVESERSGTKVITP 328
            LL PL +P               G   ++  R      + QQ++ ++E+       +  
Sbjct: 248 FLLAPLAIP------------IGVGFKELMSSR------KTQQKVHDLEAPVDKFYFVEE 307

Query: 329 CDYKEKESISRKMMGKENLTVLEEEHSAKMLVRRLDFWLYFVAYFCGGTIGLVYSNNLGQ 388
              KE+E   + ++G      ++EE     L ++LDFW+YF  Y  G T+GLV++NNLGQ
Sbjct: 308 DHTKEEEEFEKAIIG------VKEEVEWTQLWKKLDFWIYFGLYLFGPTVGLVFTNNLGQ 367

Query: 389 ISQSLGYSSLTSSLVTLYSSCSFFGRLISAGPD--FMREKVHFARTGWLALALVPNPIAF 448
           I++S G S+ TSSLV L SS  FFGRL+ +  D  F R K   +    +A +LV    +F
Sbjct: 368 IAESRG-STATSSLVALSSSFGFFGRLLPSLLDYFFSRNKYMPSSPVSMAGSLVAMVASF 427

Query: 449 ILLAASGSEIALQIGTGLIGLSSGFMFSASVSITSELFGPNSSGVNHNILITNIPLGSFL 508
           +LL    S+IAL IGT +IG+ SG + S SV++T+ELFG    GVNHNI++ +IPLGSF 
Sbjct: 428 LLLLID-SDIALYIGTAMIGIFSGALTSLSVTMTAELFGTKHFGVNHNIVVGSIPLGSFS 487

Query: 509 YGVLAAVSYDSTAGSSNQTAATLGDVVVCIGRKCYLQTFIWWACI-SIVGLASCFLLFRR 564
           +G+LAA  Y          AA  GD   C G  C+  T ++W  + SI  L +  L  R 
Sbjct: 488 FGLLAAKIY-------RDGAALYGDDGKCFGMHCFQTTLVFWGMLCSIAALLAAVLYIRN 494

The following BLAST results are available for this feature:

BLAST of CmaCh08G009830 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
F4I9E1	6.2e-156	53.53	Protein NUCLEAR FUSION DEFECTIVE 4 OS=Arabidopsis thaliana OX=3702 GN=NFD4 PE=3 ...	[more]
A0LNN5	5.0e-04	25.53	L-lactate transporter OS=Syntrophobacter fumaroxidans (strain DSM 10017 / MPOB) ...	[more]

BLAST of CmaCh08G009830 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1KEQ9	0.0e+00	100.00	protein NUCLEAR FUSION DEFECTIVE 4-like isoform X2 OS=Cucurbita maxima OX=3661 G...	[more]
A0A6J1KH74	0.0e+00	98.81	protein NUCLEAR FUSION DEFECTIVE 4-like isoform X1 OS=Cucurbita maxima OX=3661 G...	[more]
A0A6J1HJV4	0.0e+00	96.71	protein NUCLEAR FUSION DEFECTIVE 4-like isoform X2 OS=Cucurbita moschata OX=3662...	[more]
A0A6J1HGX4	0.0e+00	95.55	protein NUCLEAR FUSION DEFECTIVE 4-like isoform X1 OS=Cucurbita moschata OX=3662...	[more]
A0A1S3CCZ1	8.5e-273	83.45	protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucumis melo OX=3656 GN=LOC103499000 ...	[more]

BLAST of CmaCh08G009830 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023000026.1	0.0e+00	100.00	protein NUCLEAR FUSION DEFECTIVE 4-like isoform X2 [Cucurbita maxima]	[more]
XP_023000025.1	0.0e+00	98.81	protein NUCLEAR FUSION DEFECTIVE 4-like isoform X1 [Cucurbita maxima]	[more]
XP_022964108.1	0.0e+00	96.71	protein NUCLEAR FUSION DEFECTIVE 4-like isoform X2 [Cucurbita moschata]	[more]
XP_023515123.1	0.0e+00	96.72	protein NUCLEAR FUSION DEFECTIVE 4-like isoform X1 [Cucurbita pepo subsp. pepo]	[more]
KAG7026076.1	0.0e+00	96.19	Protein NUCLEAR FUSION DEFECTIVE 4 [Cucurbita argyrosperma subsp. argyrosperma]	[more]

BLAST of CmaCh08G009830 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G45275.1	1.2e-175	57.90	Major facilitator superfamily protein	[more]
AT4G19450.1	4.7e-175	56.16	Major facilitator superfamily protein	[more]
AT1G31470.1	4.4e-157	53.53	Major facilitator superfamily protein	[more]
AT3G01630.1	5.1e-137	47.64	Major facilitator superfamily protein	[more]
AT2G30300.1	2.4e-78	37.27	Major facilitator superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR036259	MFS transporter superfamily	GENE3D	1.20.1250.20	MFS general substrate transporter like domains	coord: 314..533 e-value: 5.4E-8 score: 33.9
IPR036259	MFS transporter superfamily	SUPERFAMILY	103473	MFS general substrate transporter	coord: 20..565
IPR010658	Nodulin-like	PFAM	PF06813	Nodulin-like	coord: 28..273 e-value: 9.7E-82 score: 274.2
None	No IPR available	PANTHER	PTHR21576:SF134	NODULIN-LIKE/MFS TRANSPORTER	coord: 23..574
None	No IPR available	PANTHER	PTHR21576	UNCHARACTERIZED NODULIN-LIKE PROTEIN	coord: 23..574

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmaCh08G009830.1	CmaCh08G009830.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane