CmaCh08G006900 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh08G006900
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
DescriptionRegulator of Vps4 activity in the MVB pathway protein, putative
LocationCma_Chr08: 4239979 .. 4242286 (-)
RNA-Seq ExpressionCmaCh08G006900
SyntenyCmaCh08G006900
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CTTCTTCTTCTTTCTCCTCCATTATATCTCTCTCGCTTTCGTTGTTCTTTTTTAAACCCTATTTTTTGGAGTTCATTTTTGTCGGTGAGCTTCTCTGTTTCTCTTGCACGGTCGTTTCTTTGTTGTATGGTTCATGTTCATCTTCCATTTGGCTCATTATTACTCGCTCCTCCTTTTGATTTTTCGTGAAATTTCTTCAATGGCGGATGTGGGATTATTCCATTTTCTTCATCCCTTCTTTTAATTGTTTTTGGAATCTTGGTTGTGGGTTTCTTGGGATTTTCATTCTCCATTGCTTGATTCTTCGGTGTTCTTCCTCGTTTCAGCCCTCTAAATTTCTGTTTTCTTTCGGATTTCTTCGGTTTCAAAATGTTCGGCATGTTCTTGAAGCCGAAGTACTATACAAAATGGTATTGATTCTGATTCTGATTCTGATTCCGATTCCGATTCTGATTTTTATGGGTTTCTTTTGATTGATTCTTTCTTTTATTGTAATGTAGCAAATCTTGCTTAAAGCTGTTGAAGTCGCGGCTGGATGCAATTAGGAAGAAGAAGAATGCGGTTCTTAAGTATTTGAAGAGCGACATTGTTGAACTTCTCAAGAGCCGTCTCGATTACAATGCTTACAACAGGGTTAGTTCTTCTTCGTGTCTCGGATTAGATTTTTAATCTCTCCGAAGCCCATAATTTTAATATCTGGTTCATCTTCCCTGATTTTGGTCGTGCTTGTTGTGCTAATGGTGAAATAGGCTGAAGGGTTTCTTGTTGAGCGAAATGTCTTGAAATGTTATGAGCTAATTGATGAATTTTGTGGGGCTATCTCCAATCAACTATCTGTCTTGATCAAACAGAGGTAGATATCATAATTTTTAGAACACGTCTGTGTTCTTAGGCTTCAGTTTGTTCAAATCAGTTTAAATGTGGTTTCCATGTTTTTGTTTCTCTTCTAGCGAATGCCCTGATGAATTCAAAGAAGCTGTTGCATCATTGATCTACGCTGCAGCGAGATTCGCCGACTTGCCTGAATTACGAGAGCTTCGAAACTTCTTTACTGGGAAATATGGATGTTCCTTCGAACCATTTACCAGCATAGAGGTTGTTCTTGGTAGCTTAAAAACCCGGTTGTGTCGTTAGAGGGTCGTCCTAAAACTTGGTTTAATTCTTGTTTTTCCTTTTAGCTTATCGAGAAGTCGAGGGCGACGATTCAAACGAAAGAGATAAAGCTTCAACTACTTCAGGAGATTGCTCAGGAATCCGCCATTGATTGGAACTCCAAAGCTCTTGAACAGCAACTGTATATTCCTCCCCCAAATGAAGTGAGTTTGTGTGCAATTCTGTTTTTGTGCGTTGATGAACTCTCTTCATTCTATTGATGGTTTTATGGTCATTCTTTTTGCAGCCTGATGTTGTTCCCATGCACGAAAGGAAGAAGGACTCGGTCGGAAAAAGGGACAGCAGCGATGACGAATCCATGTTCGATATTAGAAGTGAAGGCAACACGACCGAGACCTCGACCGTAGATAGTAGTACCGATCAAGATGTCCGAAAAGGTGGTTCCGAGGATGAAGTAGAGGATCAGACACCGTTCTACCTTAGAGTTATCCGACCTCCTTACCTTAAAACGAAACCAACCAACAAGGAAACAATGGAAGAAGCTCGAAAAGCTACCGACCAAGTCGAAAACGATGAATGTAAGAAGCAGCAACCCGAGCCAACCTCGGAAGAGAAACCGAAACCGAAACCGAAATCAGTCAGGCGAAGAAATGTGAAACTACAGCCTACAATGGAGATCAAGATTGACGATGTTGGGAATTCGGGAAACGATATGAGTTCGAGTCGAAACAAAGGTAAAGAAGCCATGAAAGGAGAAGGGGATGATGAGGAGGAGGAGGAGGAAAAGATGTTAGATGGGCTGTTGATGCATTACAGTAAGAAAAAACCAAGCCATGAATCAAACGAAGTCAGGGCAGTGTCACTGTCCATAGACTCAACTGAGCCAACTGAGCTAGAGAACAAGCGTCATACTAGAACTAATTCCTTCGTTCATCCTAATCTCCCGGACTACGACGAACTCGCAGCTCGGTTCGCAGCCCTGAAGGAAGATAAATAAAAAGTTTCGGTATCAGACGCTCGAGAAAATTATCGATCGAACGTGGTTGACATGACATTAACAAGGAGACTCCATCTAAAGGAAGATTCTTTGCTTCGAAACTAAACCAATAGGACAATTGCAATTCTCTAATCATTTCTTACAACCCACCTGTACTTTATAACTTTAAGAAAATGCAGTAAAAATAAATAAATAAAG

mRNA sequence

CTTCTTCTTCTTTCTCCTCCATTATATCTCTCTCGCTTTCGTTGTTCTTTTTTAAACCCTATTTTTTGGAGTTCATTTTTGTCGGTGAGCTTCTCTGTTTCTCTTGCACGGTCGTTTCTTTGTTGTATGGTTCATGTTCATCTTCCATTTGGCTCATTATTACTCGCTCCTCCTTTTGATTTTTCGTGAAATTTCTTCAATGGCGGATGTGGGATTATTCCATTTTCTTCATCCCTTCTTTTAATTGTTTTTGGAATCTTGGTTGTGGGTTTCTTGGGATTTTCATTCTCCATTGCTTGATTCTTCGGTGTTCTTCCTCGTTTCAGCCCTCTAAATTTCTGTTTTCTTTCGGATTTCTTCGGTTTCAAAATGTTCGGCATGTTCTTGAAGCCGAAGTACTATACAAAATGCAAATCTTGCTTAAAGCTGTTGAAGTCGCGGCTGGATGCAATTAGGAAGAAGAAGAATGCGGTTCTTAAGTATTTGAAGAGCGACATTGTTGAACTTCTCAAGAGCCGTCTCGATTACAATGCTTACAACAGGGCTGAAGGGTTTCTTGTTGAGCGAAATGTCTTGAAATGTTATGAGCTAATTGATGAATTTTGTGGGGCTATCTCCAATCAACTATCTGTCTTGATCAAACAGAGCGAATGCCCTGATGAATTCAAAGAAGCTGTTGCATCATTGATCTACGCTGCAGCGAGATTCGCCGACTTGCCTGAATTACGAGAGCTTCGAAACTTCTTTACTGGGAAATATGGATGTTCCTTCGAACCATTTACCAGCATAGAGCTTATCGAGAAGTCGAGGGCGACGATTCAAACGAAAGAGATAAAGCTTCAACTACTTCAGGAGATTGCTCAGGAATCCGCCATTGATTGGAACTCCAAAGCTCTTGAACAGCAACTGTATATTCCTCCCCCAAATGAACCTGATGTTGTTCCCATGCACGAAAGGAAGAAGGACTCGGTCGGAAAAAGGGACAGCAGCGATGACGAATCCATGTTCGATATTAGAAGTGAAGGCAACACGACCGAGACCTCGACCGTAGATAGTAGTACCGATCAAGATGTCCGAAAAGGTGGTTCCGAGGATGAAGTAGAGGATCAGACACCGTTCTACCTTAGAGTTATCCGACCTCCTTACCTTAAAACGAAACCAACCAACAAGGAAACAATGGAAGAAGCTCGAAAAGCTACCGACCAAGTCGAAAACGATGAATGTAAGAAGCAGCAACCCGAGCCAACCTCGGAAGAGAAACCGAAACCGAAACCGAAATCAGTCAGGCGAAGAAATGTGAAACTACAGCCTACAATGGAGATCAAGATTGACGATGTTGGGAATTCGGGAAACGATATGAGTTCGAGTCGAAACAAAGGTAAAGAAGCCATGAAAGGAGAAGGGGATGATGAGGAGGAGGAGGAGGAAAAGATGTTAGATGGGCTGTTGATGCATTACAGTAAGAAAAAACCAAGCCATGAATCAAACGAAGTCAGGGCAGTGTCACTGTCCATAGACTCAACTGAGCCAACTGAGCTAGAGAACAAGCGTCATACTAGAACTAATTCCTTCGTTCATCCTAATCTCCCGGACTACGACGAACTCGCAGCTCGGTTCGCAGCCCTGAAGGAAGATAAATAAAAAGTTTCGGTATCAGACGCTCGAGAAAATTATCGATCGAACGTGGTTGACATGACATTAACAAGGAGACTCCATCTAAAGGAAGATTCTTTGCTTCGAAACTAAACCAATAGGACAATTGCAATTCTCTAATCATTTCTTACAACCCACCTGTACTTTATAACTTTAAGAAAATGCAGTAAAAATAAATAAATAAAG

Coding sequence (CDS)

ATGTTCGGCATGTTCTTGAAGCCGAAGTACTATACAAAATGCAAATCTTGCTTAAAGCTGTTGAAGTCGCGGCTGGATGCAATTAGGAAGAAGAAGAATGCGGTTCTTAAGTATTTGAAGAGCGACATTGTTGAACTTCTCAAGAGCCGTCTCGATTACAATGCTTACAACAGGGCTGAAGGGTTTCTTGTTGAGCGAAATGTCTTGAAATGTTATGAGCTAATTGATGAATTTTGTGGGGCTATCTCCAATCAACTATCTGTCTTGATCAAACAGAGCGAATGCCCTGATGAATTCAAAGAAGCTGTTGCATCATTGATCTACGCTGCAGCGAGATTCGCCGACTTGCCTGAATTACGAGAGCTTCGAAACTTCTTTACTGGGAAATATGGATGTTCCTTCGAACCATTTACCAGCATAGAGCTTATCGAGAAGTCGAGGGCGACGATTCAAACGAAAGAGATAAAGCTTCAACTACTTCAGGAGATTGCTCAGGAATCCGCCATTGATTGGAACTCCAAAGCTCTTGAACAGCAACTGTATATTCCTCCCCCAAATGAACCTGATGTTGTTCCCATGCACGAAAGGAAGAAGGACTCGGTCGGAAAAAGGGACAGCAGCGATGACGAATCCATGTTCGATATTAGAAGTGAAGGCAACACGACCGAGACCTCGACCGTAGATAGTAGTACCGATCAAGATGTCCGAAAAGGTGGTTCCGAGGATGAAGTAGAGGATCAGACACCGTTCTACCTTAGAGTTATCCGACCTCCTTACCTTAAAACGAAACCAACCAACAAGGAAACAATGGAAGAAGCTCGAAAAGCTACCGACCAAGTCGAAAACGATGAATGTAAGAAGCAGCAACCCGAGCCAACCTCGGAAGAGAAACCGAAACCGAAACCGAAATCAGTCAGGCGAAGAAATGTGAAACTACAGCCTACAATGGAGATCAAGATTGACGATGTTGGGAATTCGGGAAACGATATGAGTTCGAGTCGAAACAAAGGTAAAGAAGCCATGAAAGGAGAAGGGGATGATGAGGAGGAGGAGGAGGAAAAGATGTTAGATGGGCTGTTGATGCATTACAGTAAGAAAAAACCAAGCCATGAATCAAACGAAGTCAGGGCAGTGTCACTGTCCATAGACTCAACTGAGCCAACTGAGCTAGAGAACAAGCGTCATACTAGAACTAATTCCTTCGTTCATCCTAATCTCCCGGACTACGACGAACTCGCAGCTCGGTTCGCAGCCCTGAAGGAAGATAAATAA

Protein sequence

MFGMFLKPKYYTKCKSCLKLLKSRLDAIRKKKNAVLKYLKSDIVELLKSRLDYNAYNRAEGFLVERNVLKCYELIDEFCGAISNQLSVLIKQSECPDEFKEAVASLIYAAARFADLPELRELRNFFTGKYGCSFEPFTSIELIEKSRATIQTKEIKLQLLQEIAQESAIDWNSKALEQQLYIPPPNEPDVVPMHERKKDSVGKRDSSDDESMFDIRSEGNTTETSTVDSSTDQDVRKGGSEDEVEDQTPFYLRVIRPPYLKTKPTNKETMEEARKATDQVENDECKKQQPEPTSEEKPKPKPKSVRRRNVKLQPTMEIKIDDVGNSGNDMSSSRNKGKEAMKGEGDDEEEEEEKMLDGLLMHYSKKKPSHESNEVRAVSLSIDSTEPTELENKRHTRTNSFVHPNLPDYDELAARFAALKEDK
Homology
BLAST of CmaCh08G006900 vs. ExPASy Swiss-Prot
Match: Q54I39 (IST1-like protein OS=Dictyostelium discoideum OX=44689 GN=DDB_G0289029 PE=3 SV=1)

HSP 1 Score: 80.1 bits (196), Expect = 6.6e-14
Identity = 53/194 (27.32%), Postives = 99/194 (51.03%), Query Frame = 0

Query: 1   MFGMFLKPKYYT-KCKSCLKLLKSRLDAIRKKKNAVLKYLKSDIVELLKSRLDYNAYNRA 60
           MFG    P Y + K K  LKL  SR+  ++ KK  +++  K ++ ELL+ + + +A  R 
Sbjct: 1   MFG--FGPSYDSYKLKVQLKLAVSRIQILKNKKANIVRDEKRNVAELLRKKNEESARIRV 60

Query: 61  EGFLVERNVLKCYELIDEFCGAISNQLSVLIKQSECPDEFKEAVASLIYAAARFADLPEL 120
           E  + +  +++C+++I+  C  +  +++++   +E P E KE++ +L+Y++ R   +PEL
Sbjct: 61  ETIIRDEYLIECFQIIEVLCELLHARINLINATTEMPLEMKESIFTLVYSSQRI-QIPEL 120

Query: 121 RELRNFFTGKYG--------CSFEPFTSIELIEKSRATIQTKEIKLQLLQEIAQESAIDW 180
            +++N    KYG        C      + +++ K         I  Q L EIA++  +DW
Sbjct: 121 EQIKNQLKAKYGKGLENEANCHCSTHVNPKIVHKLSYATPDPSIIFQTLSEIAEKFNVDW 180

Query: 181 NSKALEQQLYIPPP 186
                    Y PPP
Sbjct: 181 CGSD-----YPPPP 186

BLAST of CmaCh08G006900 vs. ExPASy TrEMBL
Match: A0A6J1KJN9 (uncharacterized protein LOC111494616 OS=Cucurbita maxima OX=3661 GN=LOC111494616 PE=3 SV=1)

HSP 1 Score: 810.8 bits (2093), Expect = 2.6e-231
Identity = 423/423 (100.00%), Postives = 423/423 (100.00%), Query Frame = 0

Query: 1   MFGMFLKPKYYTKCKSCLKLLKSRLDAIRKKKNAVLKYLKSDIVELLKSRLDYNAYNRAE 60
           MFGMFLKPKYYTKCKSCLKLLKSRLDAIRKKKNAVLKYLKSDIVELLKSRLDYNAYNRAE
Sbjct: 1   MFGMFLKPKYYTKCKSCLKLLKSRLDAIRKKKNAVLKYLKSDIVELLKSRLDYNAYNRAE 60

Query: 61  GFLVERNVLKCYELIDEFCGAISNQLSVLIKQSECPDEFKEAVASLIYAAARFADLPELR 120
           GFLVERNVLKCYELIDEFCGAISNQLSVLIKQSECPDEFKEAVASLIYAAARFADLPELR
Sbjct: 61  GFLVERNVLKCYELIDEFCGAISNQLSVLIKQSECPDEFKEAVASLIYAAARFADLPELR 120

Query: 121 ELRNFFTGKYGCSFEPFTSIELIEKSRATIQTKEIKLQLLQEIAQESAIDWNSKALEQQL 180
           ELRNFFTGKYGCSFEPFTSIELIEKSRATIQTKEIKLQLLQEIAQESAIDWNSKALEQQL
Sbjct: 121 ELRNFFTGKYGCSFEPFTSIELIEKSRATIQTKEIKLQLLQEIAQESAIDWNSKALEQQL 180

Query: 181 YIPPPNEPDVVPMHERKKDSVGKRDSSDDESMFDIRSEGNTTETSTVDSSTDQDVRKGGS 240
           YIPPPNEPDVVPMHERKKDSVGKRDSSDDESMFDIRSEGNTTETSTVDSSTDQDVRKGGS
Sbjct: 181 YIPPPNEPDVVPMHERKKDSVGKRDSSDDESMFDIRSEGNTTETSTVDSSTDQDVRKGGS 240

Query: 241 EDEVEDQTPFYLRVIRPPYLKTKPTNKETMEEARKATDQVENDECKKQQPEPTSEEKPKP 300
           EDEVEDQTPFYLRVIRPPYLKTKPTNKETMEEARKATDQVENDECKKQQPEPTSEEKPKP
Sbjct: 241 EDEVEDQTPFYLRVIRPPYLKTKPTNKETMEEARKATDQVENDECKKQQPEPTSEEKPKP 300

Query: 301 KPKSVRRRNVKLQPTMEIKIDDVGNSGNDMSSSRNKGKEAMKGEGDDEEEEEEKMLDGLL 360
           KPKSVRRRNVKLQPTMEIKIDDVGNSGNDMSSSRNKGKEAMKGEGDDEEEEEEKMLDGLL
Sbjct: 301 KPKSVRRRNVKLQPTMEIKIDDVGNSGNDMSSSRNKGKEAMKGEGDDEEEEEEKMLDGLL 360

Query: 361 MHYSKKKPSHESNEVRAVSLSIDSTEPTELENKRHTRTNSFVHPNLPDYDELAARFAALK 420
           MHYSKKKPSHESNEVRAVSLSIDSTEPTELENKRHTRTNSFVHPNLPDYDELAARFAALK
Sbjct: 361 MHYSKKKPSHESNEVRAVSLSIDSTEPTELENKRHTRTNSFVHPNLPDYDELAARFAALK 420

Query: 421 EDK 424
           EDK
Sbjct: 421 EDK 423

BLAST of CmaCh08G006900 vs. ExPASy TrEMBL
Match: A0A6J1HLZ1 (uncharacterized protein LOC111464187 OS=Cucurbita moschata OX=3662 GN=LOC111464187 PE=3 SV=1)

HSP 1 Score: 755.7 bits (1950), Expect = 1.0e-214
Identity = 402/426 (94.37%), Postives = 409/426 (96.01%), Query Frame = 0

Query: 1   MFGMFLKPKYYTKCKSCLKLLKSRLDAIRKKKNAVLKYLKSDIVELLKSRLDYNAYNRAE 60
           MF MFLKPKYYTKCKSCLKLLKSRLDAIRKKKN VLKYLKSDIVELLKSRLDYNAYNRAE
Sbjct: 1   MFDMFLKPKYYTKCKSCLKLLKSRLDAIRKKKNTVLKYLKSDIVELLKSRLDYNAYNRAE 60

Query: 61  GFLVERNVLKCYELIDEFCGAISNQLSVLIKQSECPDEFKEAVASLIYAAARFADLPELR 120
           GFLVERNVL+CYELIDEFCG ISNQLSVLIKQSECPDE  EAVASLIYAAARFADLPELR
Sbjct: 61  GFLVERNVLRCYELIDEFCGTISNQLSVLIKQSECPDECNEAVASLIYAAARFADLPELR 120

Query: 121 ELRNFFTGKYGCSFEPFTSIELIEKSRATIQTKEIKLQLLQEIAQESAIDWNSKALEQQL 180
           ELRNFFTGKYGCSFEPFTSIELIEKSRATIQ+KEIKLQLLQEIAQESAIDWNSKALEQQL
Sbjct: 121 ELRNFFTGKYGCSFEPFTSIELIEKSRATIQSKEIKLQLLQEIAQESAIDWNSKALEQQL 180

Query: 181 YIPPPNEPDVVPMHERKKDSVGKRDSSDDESMFDIRSEGNTTETSTVDSSTDQDVRKGGS 240
           YIPPPNEPDVVP+HERKK SVGKRDSSDDESMFDIRSEGNTTETSTVDSSTDQDVRKGGS
Sbjct: 181 YIPPPNEPDVVPVHERKKSSVGKRDSSDDESMFDIRSEGNTTETSTVDSSTDQDVRKGGS 240

Query: 241 EDEVEDQTPFYLRVIRPPYLKTKPTNKETMEEARKATDQVENDECKKQQPEPTSEEKPKP 300
           EDEVEDQTPFYLRVIRPPYLKTKPTNKETMEEARKATDQVENDE  KQQ EPTSEEKPKP
Sbjct: 241 EDEVEDQTPFYLRVIRPPYLKTKPTNKETMEEARKATDQVENDERNKQQSEPTSEEKPKP 300

Query: 301 KPKSVRRRNVKLQPTMEIKIDDVGNSGNDMSSSRNKGKEAMKGEGDDEEEEEEKMLDGLL 360
           KPKSVRRRNVKLQPT+EIKI +V NSGND+SSSRNKGKEAMKGEGDD EEEEEKMLDGLL
Sbjct: 301 KPKSVRRRNVKLQPTVEIKIGNVENSGNDISSSRNKGKEAMKGEGDD-EEEEEKMLDGLL 360

Query: 361 MHYSKKKPSHESNEVRAVSLSIDSTEP---TELENKRHTRTNSFVHPNLPDYDELAARFA 420
           MHYSKKKPSHESNEVRAVSLSIDSTEP   TELENK HTRTNSFVHP LPDYD+LAARFA
Sbjct: 361 MHYSKKKPSHESNEVRAVSLSIDSTEPTELTELENKHHTRTNSFVHPKLPDYDKLAARFA 420

Query: 421 ALKEDK 424
           ALKEDK
Sbjct: 421 ALKEDK 425

BLAST of CmaCh08G006900 vs. ExPASy TrEMBL
Match: A0A1S3CGT5 (uncharacterized protein LOC103500577 OS=Cucumis melo OX=3656 GN=LOC103500577 PE=3 SV=1)

HSP 1 Score: 508.4 bits (1308), Expect = 2.8e-140
Identity = 301/465 (64.73%), Postives = 340/465 (73.12%), Query Frame = 0

Query: 1   MFGMFLKPKYYTKCKSCLKLLKSRLDAIRKKKNAVLKYLKSDIVELLKSRLDYNAYNRAE 60
           MF MFLKPK+YTKCKSC+K+ K+RLD IRKKKNAVLKYLK+DIVELLKSRLDYNA+NRAE
Sbjct: 1   MFDMFLKPKFYTKCKSCVKMTKTRLDTIRKKKNAVLKYLKNDIVELLKSRLDYNAFNRAE 60

Query: 61  GFLVERNVLKCYELIDEFCGAISNQLSVLIKQSECPDEFKEAVASLIYAAARFADLPELR 120
           GFLVERNVL+CYELIDEFCG ISNQ+ VL K+SECPDE KEAVA+LIYAAARFADLPELR
Sbjct: 61  GFLVERNVLRCYELIDEFCGTISNQIPVLNKESECPDECKEAVATLIYAAARFADLPELR 120

Query: 121 ELRNFFTGKYGCSFEPFTSIELIEKSRATIQTKEIKLQLLQEIAQESAIDWNSKALEQQL 180
           ELRN FT KYG SF  FT+ E IEKSRAT QTKE+K+QLLQEIAQE+AIDWNSKALEQQL
Sbjct: 121 ELRNLFTEKYGSSFGSFTNKEFIEKSRATTQTKEMKIQLLQEIAQETAIDWNSKALEQQL 180

Query: 181 YIPPP-NEPD---------------VVPMHERKKDSVGKRDSSDDESMFDIRSEGNTTET 240
           Y PPP NE D                VP++ERK +S   +++SD+ES+FD RSEGNTTET
Sbjct: 181 YTPPPQNELDGERSGATKRNKTKVVSVPVYERKANSPRNKNNSDNESIFDSRSEGNTTET 240

Query: 241 STVDSSTDQDVRKGGSEDEVEDQTPFYLRVIRPPYLKTKPTNKETME-EARKATDQVEND 300
           ST D STDQD+ KG SEDEVEDQ PF  R ++PPYLKTKP   E  E E RK T  +END
Sbjct: 241 STGD-STDQDIHKGVSEDEVEDQKPFNHRFVQPPYLKTKPIKTEANEKEPRKVT--IEND 300

Query: 301 ECKKQQPEPTSEEKPKPKPKSVRRRNVKLQPTMEIKIDDVGNSGND----MSSSRNKGKE 360
           E       P S  + KPKP+SVRRRNVK QP  +I IDDVG+S  D    +SSSRNKGKE
Sbjct: 301 ESN----NPKSPTEEKPKPRSVRRRNVKPQPARDINIDDVGSSTIDVTTKISSSRNKGKE 360

Query: 361 AMKGEGDDEEEEEEKMLDGLLMHYSKKKPSHESNE-----------------------VR 420
            M GE     ++EE++LDGLLM YSKKK + ES                          R
Sbjct: 361 TMIGEEKGARDDEERVLDGLLMQYSKKKTNQESKSRGKSNLKPQRQQEKDNIEHQRPTTR 420

Query: 421 AVSLSIDSTEPTELENKRHTRTNSFVHPNLPDYDELAARFAALKE 422
           AVSL  D  E T    K+HTRTNSFVHP LP+YD+LAAR AALKE
Sbjct: 421 AVSLPTDQNEST----KKHTRTNSFVHPKLPEYDQLAARIAALKE 454

BLAST of CmaCh08G006900 vs. ExPASy TrEMBL
Match: A0A6J1CWS8 (vacuolar protein sorting-associated protein IST1 OS=Momordica charantia OX=3673 GN=LOC111015509 PE=3 SV=1)

HSP 1 Score: 488.0 bits (1255), Expect = 3.9e-134
Identity = 291/470 (61.91%), Postives = 326/470 (69.36%), Query Frame = 0

Query: 1   MFGMFLKPKYYTKCKSCLKLLKSRLDAIRKKKNAVLKYLKSDIVELLKSRLDYNAYNRAE 60
           MF MF KPK++TKCKSC+KLLK RLD IRKKKNAVLK+LK+DI ELL+S LD NAYNRAE
Sbjct: 1   MFDMFFKPKFFTKCKSCVKLLKMRLDTIRKKKNAVLKFLKNDIAELLRSGLDQNAYNRAE 60

Query: 61  GFLVERNVLKCYELIDEFCGAISNQLSVLIKQSECPDEFKEAVASLIYAAARFADLPELR 120
           G LVERNVLKCY+LIDEF G I NQLSVL KQSECPDE +EAVASLIYAAARFADLPELR
Sbjct: 61  GLLVERNVLKCYDLIDEFSGTIYNQLSVLSKQSECPDECREAVASLIYAAARFADLPELR 120

Query: 121 ELRNFFTGKYGCSFEPFTSIELIEKSRATIQTKEIKLQLLQEIAQESAIDWNSKALEQQL 180
           ELR+ FT KYG SFE FT+ ELIEK  A   TKE+KLQLLQEIAQES I+WNSKALEQQL
Sbjct: 121 ELRSLFTAKYGRSFEQFTNKELIEKPSAMAMTKEVKLQLLQEIAQESGIEWNSKALEQQL 180

Query: 181 YIPPPNEPD--------------VVPMHERKKDSVGKRDSSDDESMFDIRSEGNTTETST 240
           Y PP N PD               +P+ ERK   + K+D+SDD+S+FD RSE N TETST
Sbjct: 181 YTPPQNTPDEEPNADRRNKAKVVSIPVFERKTSLLQKKDNSDDDSIFDSRSEDNGTETST 240

Query: 241 VDSSTDQDVRKGGSEDEVEDQTPFYLRVIRPPYLKTKPTNKETMEEARKATDQVENDECK 300
            DSSTDQDV+KG  EDEVED+ PFYLR I PPYLK+KP+ KE   E              
Sbjct: 241 GDSSTDQDVQKGVFEDEVEDRKPFYLRFIPPPYLKSKPSKKEASFE-------------- 300

Query: 301 KQQPEPTSEEKPKPKPKSVRRRNVKLQPTMEIKIDDVGNSGND----MSSSRNKGKEAMK 360
               +P  EE  KPKP+SVRRRN KL+PT +I I +V NS ND     SSSRNKGKE M 
Sbjct: 301 ----KPAVEE--KPKPRSVRRRNSKLEPTRDINIGNVDNSANDGTEKASSSRNKGKETMM 360

Query: 361 GEGDDEEEEEEKMLDGLLMHYSKKKPSHESNEV--------------------------- 420
           GE +    EEE+MLDGLLMHYSKKK + ESN                             
Sbjct: 361 GE-EKGAGEEERMLDGLLMHYSKKKSTQESNRAKGNLKSQKHKEQDEPQRTKNSKAEEDL 420

Query: 421 ----RAVSLSIDSTEPTELENKRHTRTNSFVHPNLPDYDELAARFAALKE 422
               R+VSL  D+ EP  +  KRHTR NSFVHP LPDYD+LAARFAALKE
Sbjct: 421 SVPGRSVSLPRDTNEPI-VPMKRHTRNNSFVHPKLPDYDQLAARFAALKE 448

BLAST of CmaCh08G006900 vs. ExPASy TrEMBL
Match: A0A6J1GMM1 (uncharacterized protein LOC111455825 OS=Cucurbita moschata OX=3662 GN=LOC111455825 PE=3 SV=1)

HSP 1 Score: 487.3 bits (1253), Expect = 6.6e-134
Identity = 287/460 (62.39%), Postives = 330/460 (71.74%), Query Frame = 0

Query: 1   MFGMFLKPKYYTKCKSCLKLLKSRLDAIRKKKNAVLKYLKSDIVELLKSRLDYNAYNRAE 60
           MF MF KPK+YTKCKSC+K+ K+RLD IRKKK  VLK+LK+DIVELLKSRLDYNAYNRAE
Sbjct: 1   MFRMFFKPKFYTKCKSCVKMTKTRLDTIRKKKKVVLKFLKNDIVELLKSRLDYNAYNRAE 60

Query: 61  GFLVERNVLKCYELIDEFCGAISNQLSVLIKQSECPDEFKEAVASLIYAAARFADLPELR 120
           GFLVE+NVL+CY+LIDEFCG I   +SVL K+SECPDE KEAVASLIYAAARF+DLPELR
Sbjct: 61  GFLVEQNVLRCYDLIDEFCGTIFKHISVLNKESECPDECKEAVASLIYAAARFSDLPELR 120

Query: 121 ELRNFFTGKYGCSFEPFTSIELIEKSRATIQTKEIKLQLLQEIAQESAIDWNSKALEQQL 180
            LR+ FT +YG SF  FT+ EL+EK RA  QTKE KLQLLQEIAQESAIDWNSKALEQQL
Sbjct: 121 VLRSLFTERYGTSFGSFTNKELVEKLRAVAQTKETKLQLLQEIAQESAIDWNSKALEQQL 180

Query: 181 YIPPPNEPDV---------------VPMHERKKDSVGKRDSSDDESMFDIRSEGNTTETS 240
           Y+PP NE D                VP++ R  +S  K+D+SDD+S+FD RSEGNTTETS
Sbjct: 181 YVPPQNELDCERSTSTCRSKSKIVSVPVYGRTTNSPRKKDNSDDDSIFDSRSEGNTTETS 240

Query: 241 TVDSSTDQDVRKG-GSEDEVEDQTPFYLRVIRPPYLKTKPTNK-ETMEEARKATDQVEND 300
             DSSTDQDV KG  SEDEVEDQ PFYLR I PPYLKTKPT K E +EE +  T++  ND
Sbjct: 241 MGDSSTDQDVHKGVVSEDEVEDQKPFYLRFITPPYLKTKPTKKEENVEEPQNDTEENRND 300

Query: 301 ECKKQQPEPTSEEKPKPKPKSVRRRNVKLQPTMEIKIDDVGNSGND----MSSSRNKGKE 360
                 P+   EE  KPKP+SVRRR+ K QP   I IDD G S  D    +SSSRNKGKE
Sbjct: 301 ------PKTAIEE--KPKPRSVRRRHAKPQPARSINIDDAGGSAADGAGKISSSRNKGKE 360

Query: 361 AMKGEGDDEEEEEEKMLDGLLMHYSKKKPSHESNE------------------VRAVSLS 420
           AM GE    E++EE+MLDGLL  YSKKK   E  +                   +++SL 
Sbjct: 361 AMTGEEKGVEDDEERMLDGLLKLYSKKKTRQEDKDNAEPQRPKNTRTEDAYVPTKSLSLP 420

Query: 421 IDSTEPTELENKRHTRTNSFVHPNLPDYDELAARFAALKE 422
              TEP E   K+H R NS+VHP LPDYD+LAARFA+LKE
Sbjct: 421 PPRTEPIE-PVKKHARANSYVHPKLPDYDQLAARFASLKE 451

BLAST of CmaCh08G006900 vs. NCBI nr
Match: XP_023000359.1 (uncharacterized protein LOC111494616 [Cucurbita maxima])

HSP 1 Score: 810.8 bits (2093), Expect = 5.4e-231
Identity = 423/423 (100.00%), Postives = 423/423 (100.00%), Query Frame = 0

Query: 1   MFGMFLKPKYYTKCKSCLKLLKSRLDAIRKKKNAVLKYLKSDIVELLKSRLDYNAYNRAE 60
           MFGMFLKPKYYTKCKSCLKLLKSRLDAIRKKKNAVLKYLKSDIVELLKSRLDYNAYNRAE
Sbjct: 1   MFGMFLKPKYYTKCKSCLKLLKSRLDAIRKKKNAVLKYLKSDIVELLKSRLDYNAYNRAE 60

Query: 61  GFLVERNVLKCYELIDEFCGAISNQLSVLIKQSECPDEFKEAVASLIYAAARFADLPELR 120
           GFLVERNVLKCYELIDEFCGAISNQLSVLIKQSECPDEFKEAVASLIYAAARFADLPELR
Sbjct: 61  GFLVERNVLKCYELIDEFCGAISNQLSVLIKQSECPDEFKEAVASLIYAAARFADLPELR 120

Query: 121 ELRNFFTGKYGCSFEPFTSIELIEKSRATIQTKEIKLQLLQEIAQESAIDWNSKALEQQL 180
           ELRNFFTGKYGCSFEPFTSIELIEKSRATIQTKEIKLQLLQEIAQESAIDWNSKALEQQL
Sbjct: 121 ELRNFFTGKYGCSFEPFTSIELIEKSRATIQTKEIKLQLLQEIAQESAIDWNSKALEQQL 180

Query: 181 YIPPPNEPDVVPMHERKKDSVGKRDSSDDESMFDIRSEGNTTETSTVDSSTDQDVRKGGS 240
           YIPPPNEPDVVPMHERKKDSVGKRDSSDDESMFDIRSEGNTTETSTVDSSTDQDVRKGGS
Sbjct: 181 YIPPPNEPDVVPMHERKKDSVGKRDSSDDESMFDIRSEGNTTETSTVDSSTDQDVRKGGS 240

Query: 241 EDEVEDQTPFYLRVIRPPYLKTKPTNKETMEEARKATDQVENDECKKQQPEPTSEEKPKP 300
           EDEVEDQTPFYLRVIRPPYLKTKPTNKETMEEARKATDQVENDECKKQQPEPTSEEKPKP
Sbjct: 241 EDEVEDQTPFYLRVIRPPYLKTKPTNKETMEEARKATDQVENDECKKQQPEPTSEEKPKP 300

Query: 301 KPKSVRRRNVKLQPTMEIKIDDVGNSGNDMSSSRNKGKEAMKGEGDDEEEEEEKMLDGLL 360
           KPKSVRRRNVKLQPTMEIKIDDVGNSGNDMSSSRNKGKEAMKGEGDDEEEEEEKMLDGLL
Sbjct: 301 KPKSVRRRNVKLQPTMEIKIDDVGNSGNDMSSSRNKGKEAMKGEGDDEEEEEEKMLDGLL 360

Query: 361 MHYSKKKPSHESNEVRAVSLSIDSTEPTELENKRHTRTNSFVHPNLPDYDELAARFAALK 420
           MHYSKKKPSHESNEVRAVSLSIDSTEPTELENKRHTRTNSFVHPNLPDYDELAARFAALK
Sbjct: 361 MHYSKKKPSHESNEVRAVSLSIDSTEPTELENKRHTRTNSFVHPNLPDYDELAARFAALK 420

Query: 421 EDK 424
           EDK
Sbjct: 421 EDK 423

BLAST of CmaCh08G006900 vs. NCBI nr
Match: KAG6593450.1 (IST1-like protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 760.0 bits (1961), Expect = 1.1e-215
Identity = 401/423 (94.80%), Postives = 409/423 (96.69%), Query Frame = 0

Query: 1   MFGMFLKPKYYTKCKSCLKLLKSRLDAIRKKKNAVLKYLKSDIVELLKSRLDYNAYNRAE 60
           MF MFLKPKYYTKCKSCLKLLKSRLDAIRKKKN VLKYLKSDIVELLKSRLDYNAYNRAE
Sbjct: 1   MFDMFLKPKYYTKCKSCLKLLKSRLDAIRKKKNTVLKYLKSDIVELLKSRLDYNAYNRAE 60

Query: 61  GFLVERNVLKCYELIDEFCGAISNQLSVLIKQSECPDEFKEAVASLIYAAARFADLPELR 120
           GFLVERNVL+CYELIDEFCG ISNQLSVLIKQSECPDE  EAVASLIYAAARFADLPELR
Sbjct: 61  GFLVERNVLRCYELIDEFCGTISNQLSVLIKQSECPDECNEAVASLIYAAARFADLPELR 120

Query: 121 ELRNFFTGKYGCSFEPFTSIELIEKSRATIQTKEIKLQLLQEIAQESAIDWNSKALEQQL 180
           ELRNFFTGKYGCSFEPFTSIELIEKSRATIQ+KEIKLQLLQEIAQESAIDWNSKALEQQL
Sbjct: 121 ELRNFFTGKYGCSFEPFTSIELIEKSRATIQSKEIKLQLLQEIAQESAIDWNSKALEQQL 180

Query: 181 YIPPPNEPDVVPMHERKKDSVGKRDSSDDESMFDIRSEGNTTETSTVDSSTDQDVRKGGS 240
           YIPPPNEPDVVP+HERKK SVGKRDSSDDESMFDIRSEGNTTETSTVDSSTDQDVRKGGS
Sbjct: 181 YIPPPNEPDVVPVHERKKSSVGKRDSSDDESMFDIRSEGNTTETSTVDSSTDQDVRKGGS 240

Query: 241 EDEVEDQTPFYLRVIRPPYLKTKPTNKETMEEARKATDQVENDECKKQQPEPTSEEKPKP 300
           EDEVEDQTPFYLRVIRPPYLKTKPTNKETMEEARKATDQVENDE  KQQ EPTSEEKPKP
Sbjct: 241 EDEVEDQTPFYLRVIRPPYLKTKPTNKETMEEARKATDQVENDERNKQQSEPTSEEKPKP 300

Query: 301 KPKSVRRRNVKLQPTMEIKIDDVGNSGNDMSSSRNKGKEAMKGEGDDEEEEEEKMLDGLL 360
           KPKSVRRRNVKLQPT+EIKI +V NSGND+SSSRNKGKEAMKGEGDD +EEEEKMLDGLL
Sbjct: 301 KPKSVRRRNVKLQPTVEIKIGNVENSGNDISSSRNKGKEAMKGEGDD-DEEEEKMLDGLL 360

Query: 361 MHYSKKKPSHESNEVRAVSLSIDSTEPTELENKRHTRTNSFVHPNLPDYDELAARFAALK 420
           MHYSKKKPSHESNEVRAVSLSIDSTEPTELENK HTRTNSFVHP LPDYD+LAARFAALK
Sbjct: 361 MHYSKKKPSHESNEVRAVSLSIDSTEPTELENKHHTRTNSFVHPKLPDYDKLAARFAALK 420

Query: 421 EDK 424
           EDK
Sbjct: 421 EDK 422

BLAST of CmaCh08G006900 vs. NCBI nr
Match: XP_022964044.1 (uncharacterized protein LOC111464187 [Cucurbita moschata] >KAG7025791.1 IST1-like protein [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 755.7 bits (1950), Expect = 2.1e-214
Identity = 402/426 (94.37%), Postives = 409/426 (96.01%), Query Frame = 0

Query: 1   MFGMFLKPKYYTKCKSCLKLLKSRLDAIRKKKNAVLKYLKSDIVELLKSRLDYNAYNRAE 60
           MF MFLKPKYYTKCKSCLKLLKSRLDAIRKKKN VLKYLKSDIVELLKSRLDYNAYNRAE
Sbjct: 1   MFDMFLKPKYYTKCKSCLKLLKSRLDAIRKKKNTVLKYLKSDIVELLKSRLDYNAYNRAE 60

Query: 61  GFLVERNVLKCYELIDEFCGAISNQLSVLIKQSECPDEFKEAVASLIYAAARFADLPELR 120
           GFLVERNVL+CYELIDEFCG ISNQLSVLIKQSECPDE  EAVASLIYAAARFADLPELR
Sbjct: 61  GFLVERNVLRCYELIDEFCGTISNQLSVLIKQSECPDECNEAVASLIYAAARFADLPELR 120

Query: 121 ELRNFFTGKYGCSFEPFTSIELIEKSRATIQTKEIKLQLLQEIAQESAIDWNSKALEQQL 180
           ELRNFFTGKYGCSFEPFTSIELIEKSRATIQ+KEIKLQLLQEIAQESAIDWNSKALEQQL
Sbjct: 121 ELRNFFTGKYGCSFEPFTSIELIEKSRATIQSKEIKLQLLQEIAQESAIDWNSKALEQQL 180

Query: 181 YIPPPNEPDVVPMHERKKDSVGKRDSSDDESMFDIRSEGNTTETSTVDSSTDQDVRKGGS 240
           YIPPPNEPDVVP+HERKK SVGKRDSSDDESMFDIRSEGNTTETSTVDSSTDQDVRKGGS
Sbjct: 181 YIPPPNEPDVVPVHERKKSSVGKRDSSDDESMFDIRSEGNTTETSTVDSSTDQDVRKGGS 240

Query: 241 EDEVEDQTPFYLRVIRPPYLKTKPTNKETMEEARKATDQVENDECKKQQPEPTSEEKPKP 300
           EDEVEDQTPFYLRVIRPPYLKTKPTNKETMEEARKATDQVENDE  KQQ EPTSEEKPKP
Sbjct: 241 EDEVEDQTPFYLRVIRPPYLKTKPTNKETMEEARKATDQVENDERNKQQSEPTSEEKPKP 300

Query: 301 KPKSVRRRNVKLQPTMEIKIDDVGNSGNDMSSSRNKGKEAMKGEGDDEEEEEEKMLDGLL 360
           KPKSVRRRNVKLQPT+EIKI +V NSGND+SSSRNKGKEAMKGEGDD EEEEEKMLDGLL
Sbjct: 301 KPKSVRRRNVKLQPTVEIKIGNVENSGNDISSSRNKGKEAMKGEGDD-EEEEEKMLDGLL 360

Query: 361 MHYSKKKPSHESNEVRAVSLSIDSTEP---TELENKRHTRTNSFVHPNLPDYDELAARFA 420
           MHYSKKKPSHESNEVRAVSLSIDSTEP   TELENK HTRTNSFVHP LPDYD+LAARFA
Sbjct: 361 MHYSKKKPSHESNEVRAVSLSIDSTEPTELTELENKHHTRTNSFVHPKLPDYDKLAARFA 420

Query: 421 ALKEDK 424
           ALKEDK
Sbjct: 421 ALKEDK 425

BLAST of CmaCh08G006900 vs. NCBI nr
Match: XP_023514654.1 (uncharacterized protein LOC111778886 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 751.5 bits (1939), Expect = 3.9e-213
Identity = 402/426 (94.37%), Postives = 408/426 (95.77%), Query Frame = 0

Query: 1   MFGMFLKPKYYTKCKSCLKLLKSRLDAIRKKKNAVLKYLKSDIVELLKSRLDYNAYNRAE 60
           MF MFLKPKYYTKCKSCLKLLKSRLDAIRKKKNAVLKYLKSDIVELLKSRLDYNAYNRAE
Sbjct: 1   MFDMFLKPKYYTKCKSCLKLLKSRLDAIRKKKNAVLKYLKSDIVELLKSRLDYNAYNRAE 60

Query: 61  GFLVERNVLKCYELIDEFCGAISNQLSVLIKQSECPDEFKEAVASLIYAAARFADLPELR 120
           GFLVERNVL+CYELIDEFCG ISNQLSVLIKQSECPDE  EAVASLIYAAARFADLPELR
Sbjct: 61  GFLVERNVLRCYELIDEFCGTISNQLSVLIKQSECPDECNEAVASLIYAAARFADLPELR 120

Query: 121 ELRNFFTGKYGCSFEPFTSIELIEKSRATIQTKEIKLQLLQEIAQESAIDWNSKALEQQL 180
           ELRNFFTGKYGCSFEPFTSIELIEKSRATIQ+KEIKLQLLQEIAQESAIDWNSKALEQQL
Sbjct: 121 ELRNFFTGKYGCSFEPFTSIELIEKSRATIQSKEIKLQLLQEIAQESAIDWNSKALEQQL 180

Query: 181 YIPPPNEPDVVPMHERKKDSVGKRDSSDDESMFDIRSEGNTTETSTVDSSTDQDVRKGGS 240
           YIPPPNEPDVVP+H RKK SVGKRDSSDDESMFDIRSEGNTTETSTVDSSTDQDVRKGGS
Sbjct: 181 YIPPPNEPDVVPVHGRKKSSVGKRDSSDDESMFDIRSEGNTTETSTVDSSTDQDVRKGGS 240

Query: 241 EDEVEDQTPFYLRVIRPPYLKTKPTNKETMEEARKATDQVENDECKKQQPEPTSEEKPKP 300
           EDEVEDQTPFYLRVIRPPYLKTKPTNKETMEEARKAT+QVENDE  KQQPEPT EE  KP
Sbjct: 241 EDEVEDQTPFYLRVIRPPYLKTKPTNKETMEEARKATNQVENDERNKQQPEPTLEE--KP 300

Query: 301 KPKSVRRRNVKLQPTMEIKIDDVGNSGNDMSSSRNKGKEAMKGEGDDEEEEEEKMLDGLL 360
           KPKSVRRRNVKLQPTMEIKID+VGNSGNDMSSSRNKGKEAMKGEGDD +EEEEKMLDGLL
Sbjct: 301 KPKSVRRRNVKLQPTMEIKIDNVGNSGNDMSSSRNKGKEAMKGEGDD-DEEEEKMLDGLL 360

Query: 361 MHYSKKKPSHESNEVRAVSLSIDSTEP---TELENKRHTRTNSFVHPNLPDYDELAARFA 420
           MHYSKKKPSHESNEVRAVSLSIDSTEP   TELENK H RTNSFVHP LPDYDELAARFA
Sbjct: 361 MHYSKKKPSHESNEVRAVSLSIDSTEPTELTELENKHHIRTNSFVHPKLPDYDELAARFA 420

Query: 421 ALKEDK 424
           ALKEDK
Sbjct: 421 ALKEDK 423

BLAST of CmaCh08G006900 vs. NCBI nr
Match: XP_008462153.1 (PREDICTED: uncharacterized protein LOC103500577 [Cucumis melo])

HSP 1 Score: 508.4 bits (1308), Expect = 5.7e-140
Identity = 301/465 (64.73%), Postives = 340/465 (73.12%), Query Frame = 0

Query: 1   MFGMFLKPKYYTKCKSCLKLLKSRLDAIRKKKNAVLKYLKSDIVELLKSRLDYNAYNRAE 60
           MF MFLKPK+YTKCKSC+K+ K+RLD IRKKKNAVLKYLK+DIVELLKSRLDYNA+NRAE
Sbjct: 1   MFDMFLKPKFYTKCKSCVKMTKTRLDTIRKKKNAVLKYLKNDIVELLKSRLDYNAFNRAE 60

Query: 61  GFLVERNVLKCYELIDEFCGAISNQLSVLIKQSECPDEFKEAVASLIYAAARFADLPELR 120
           GFLVERNVL+CYELIDEFCG ISNQ+ VL K+SECPDE KEAVA+LIYAAARFADLPELR
Sbjct: 61  GFLVERNVLRCYELIDEFCGTISNQIPVLNKESECPDECKEAVATLIYAAARFADLPELR 120

Query: 121 ELRNFFTGKYGCSFEPFTSIELIEKSRATIQTKEIKLQLLQEIAQESAIDWNSKALEQQL 180
           ELRN FT KYG SF  FT+ E IEKSRAT QTKE+K+QLLQEIAQE+AIDWNSKALEQQL
Sbjct: 121 ELRNLFTEKYGSSFGSFTNKEFIEKSRATTQTKEMKIQLLQEIAQETAIDWNSKALEQQL 180

Query: 181 YIPPP-NEPD---------------VVPMHERKKDSVGKRDSSDDESMFDIRSEGNTTET 240
           Y PPP NE D                VP++ERK +S   +++SD+ES+FD RSEGNTTET
Sbjct: 181 YTPPPQNELDGERSGATKRNKTKVVSVPVYERKANSPRNKNNSDNESIFDSRSEGNTTET 240

Query: 241 STVDSSTDQDVRKGGSEDEVEDQTPFYLRVIRPPYLKTKPTNKETME-EARKATDQVEND 300
           ST D STDQD+ KG SEDEVEDQ PF  R ++PPYLKTKP   E  E E RK T  +END
Sbjct: 241 STGD-STDQDIHKGVSEDEVEDQKPFNHRFVQPPYLKTKPIKTEANEKEPRKVT--IEND 300

Query: 301 ECKKQQPEPTSEEKPKPKPKSVRRRNVKLQPTMEIKIDDVGNSGND----MSSSRNKGKE 360
           E       P S  + KPKP+SVRRRNVK QP  +I IDDVG+S  D    +SSSRNKGKE
Sbjct: 301 ESN----NPKSPTEEKPKPRSVRRRNVKPQPARDINIDDVGSSTIDVTTKISSSRNKGKE 360

Query: 361 AMKGEGDDEEEEEEKMLDGLLMHYSKKKPSHESNE-----------------------VR 420
            M GE     ++EE++LDGLLM YSKKK + ES                          R
Sbjct: 361 TMIGEEKGARDDEERVLDGLLMQYSKKKTNQESKSRGKSNLKPQRQQEKDNIEHQRPTTR 420

Query: 421 AVSLSIDSTEPTELENKRHTRTNSFVHPNLPDYDELAARFAALKE 422
           AVSL  D  E T    K+HTRTNSFVHP LP+YD+LAAR AALKE
Sbjct: 421 AVSLPTDQNEST----KKHTRTNSFVHPKLPEYDQLAARIAALKE 454

BLAST of CmaCh08G006900 vs. TAIR 10
Match: AT1G79910.1 (Regulator of Vps4 activity in the MVB pathway protein )

HSP 1 Score: 224.9 bits (572), Expect = 1.2e-58
Identity = 141/357 (39.50%), Postives = 213/357 (59.66%), Query Frame = 0

Query: 1   MFGMFLKPKYYTKCKSCLKLLKSRLDAIRKKKNAVLKYLKSDIVELLKSRLDYNAYNRAE 60
           MF    KPK+YTKCKS +K+ K+R+D +++KKN+V KYLK+DIV+LLK+ LDYNAY RAE
Sbjct: 1   MFDGLFKPKFYTKCKSLVKITKTRVDTVKRKKNSVCKYLKNDIVDLLKNSLDYNAYGRAE 60

Query: 61  GFLVERNVLKCYELIDEFCGAISNQLSVLIKQSECPDEFKEAVASLIYAAARFADLPELR 120
           G + E+  L CYE +++FC  +++ +S+L K   CPDE +EA++SL+YAAAR +++PELR
Sbjct: 61  GLIEEKRRLACYEFLEQFCNCVASNVSLLQKSIRCPDECREAISSLVYAAARVSEVPELR 120

Query: 121 ELRNFFTGKYGCSFEPFTSIELIEKSRATIQTKEIKLQLLQEIAQESAIDWNSKALEQQL 180
           +LR+ F  +YG + + F + E +E+ +A   +KE+K++LLQEIA+E +I W++K+LEQ+L
Sbjct: 121 DLRSLFAERYGNTLDQFVNPEFVERFKAEPPSKEMKVELLQEIAREYSIKWDAKSLEQRL 180

Query: 181 YIPP--------PNEPDVVPMHERKKDSVGKRDSSDDESMFDIRSEGNTT--ETSTVDSS 240
           Y PP          EP     +   + ++ KR S       D   + N +   TS  DS 
Sbjct: 181 YTPPHHHHVHTETEEPKTEKTNSEPEKTMEKRSSLSFHGREDSMDDSNKSIMSTSEEDSM 240

Query: 241 TDQDVRKGGSEDEVEDQTPFYLRVIRP-PYLKTKPTNKETMEEARKATDQVENDECKKQQ 300
           +       G ED+ E   PFY R + P PY K K   +E++ E    +  ++ +      
Sbjct: 241 STSGSCMTGPEDDPE-MKPFYYRFMTPAPYTKPKIEKQESLPEKMTTSHLMDTE------ 300

Query: 301 PEPTSEEKPKPKPKSVRRRNVKLQPTMEIKIDDVG---NSGNDMSSSRNKGKEAMKG 344
             PT     KPKP+SVRRR     P  E + + +     S N M +S + GK + +G
Sbjct: 301 -SPTG---GKPKPRSVRRRFANPPPEAETEGEALKLKVESKNMMRASESMGKASGEG 346

BLAST of CmaCh08G006900 vs. TAIR 10
Match: AT1G79910.2 (Regulator of Vps4 activity in the MVB pathway protein )

HSP 1 Score: 149.1 bits (375), Expect = 8.2e-36
Identity = 104/298 (34.90%), Postives = 164/298 (55.03%), Query Frame = 0

Query: 60  EGFLVERNVLKCYELIDEFCGAISNQLSVLIKQSECPDEFKEAVASLIYAAARFADLPEL 119
           EG + E+  L CYE +++FC  +++ +S+L K   CPDE +EA++SL+YAAAR +++PEL
Sbjct: 46  EGLIEEKRRLACYEFLEQFCNCVASNVSLLQKSIRCPDECREAISSLVYAAARVSEVPEL 105

Query: 120 RELRNFFTGKYGCSFEPFTSIELIEKSRATIQTKEIKLQLLQEIAQESAIDWNSKALEQQ 179
           R+LR+ F  +YG + + F + E +E+ +A   +KE+K++LLQEIA+E +I W++K+LEQ+
Sbjct: 106 RDLRSLFAERYGNTLDQFVNPEFVERFKAEPPSKEMKVELLQEIAREYSIKWDAKSLEQR 165

Query: 180 LYIPP--------PNEPDVVPMHERKKDSVGKRDSSDDESMFDIRSEGNTT--ETSTVDS 239
           LY PP          EP     +   + ++ KR S       D   + N +   TS  DS
Sbjct: 166 LYTPPHHHHVHTETEEPKTEKTNSEPEKTMEKRSSLSFHGREDSMDDSNKSIMSTSEEDS 225

Query: 240 STDQDVRKGGSEDEVEDQTPFYLRVIRP-PYLKTKPTNKETMEEARKATDQVENDECKKQ 299
            +       G ED+ E   PFY R + P PY K K   +E++ E    +  ++ +     
Sbjct: 226 MSTSGSCMTGPEDDPE-MKPFYYRFMTPAPYTKPKIEKQESLPEKMTTSHLMDTE----- 285

Query: 300 QPEPTSEEKPKPKPKSVRRRNVKLQPTMEIKIDDVG---NSGNDMSSSRNKGKEAMKG 344
              PT     KPKP+SVRRR     P  E + + +     S N M +S + GK + +G
Sbjct: 286 --SPTG---GKPKPRSVRRRFANPPPEAETEGEALKLKVESKNMMRASESMGKASGEG 332

BLAST of CmaCh08G006900 vs. TAIR 10
Match: AT1G52315.1 (Regulator of Vps4 activity in the MVB pathway protein )

HSP 1 Score: 139.0 bits (349), Expect = 8.5e-33
Identity = 102/297 (34.34%), Postives = 163/297 (54.88%), Query Frame = 0

Query: 1   MFGMFLKPKYYTKCKSCLKLLKSRLDAIRKKKNAVLKYLKSDIVELLKSRLDYNAYNRAE 60
           MF    KPK+Y K KS    +K R+D +R+K+ A+++  K+DIV  LK+  D  AY RAE
Sbjct: 1   MFLRCFKPKFYKKSKSTTSYMKIRIDIVRRKRIAMVRNYKTDIVNFLKNGQDSEAYRRAE 60

Query: 61  GFLVERNVLKCYELIDEFCGAISNQLSVLIKQSECPDEFKEAVASLIYAAARFADLPELR 120
             L E  ++ CY+LI+ FC  IS  LS+++K+ ECP+E +EAV+SLIYA A   D+PEL+
Sbjct: 61  LLLEELRIISCYDLIERFCDCISENLSLMLKKRECPEECREAVSSLIYATAWVPDVPELK 120

Query: 121 ELRNFFTGKYGCSFEPFTSIELIEKSR-ATIQTKEIKLQLLQEIAQESAIDWNSKALEQQ 180
           +LR  FT ++G       + EL+EK+      ++E+K+Q ++++A E +I+W+   L+  
Sbjct: 121 DLRAVFTKRFGNFIASSVNHELVEKTELLRPPSRELKIQTVKDVANEFSINWDPTPLKLM 180

Query: 181 LYIPPPNEPDVVPMHERKKDSVGK-------RDSSDDESM------FDIRSEGNTTETST 240
           L +   +   +    E + D + K        + SDDES+       D  S+G+ + +S+
Sbjct: 181 L-LRETSALQIKDKVETRADYLNKDTEKSFMDEHSDDESVLSESWRIDSLSKGSLSSSSS 240

Query: 241 VDSSTDQDVRKGGSEDEVEDQTPFYLRVIRPPYLKTKPTN----KETMEEARKATDQ 280
             SS+ + V    S  + + +      +I  P  K    N    +E  EE RK  DQ
Sbjct: 241 SSSSSLRRV----SVRKKKGKKILPYGIISSPDTKLGAGNDEKAQEEKEEKRKIADQ 292

BLAST of CmaCh08G006900 vs. TAIR 10
Match: AT4G32350.1 (Regulator of Vps4 activity in the MVB pathway protein )

HSP 1 Score: 137.5 bits (345), Expect = 2.5e-32
Identity = 71/180 (39.44%), Postives = 117/180 (65.00%), Query Frame = 0

Query: 1   MFGMFLKPKYYTKCKSCLKLLKSRLDAIRKKKNAVLKYLKSDIVELLKSRLDYNAYNRAE 60
           MF  FL   +  K K  +KL K+R+D +R+K+NA +K+LK D+ +L+ +  DYNA++RA 
Sbjct: 1   MFDGFLGRGFAPKGKPLIKLTKNRIDVLRRKRNATIKFLKRDLADLIINGHDYNAFSRAG 60

Query: 61  GFLVERNVLKCYELIDEFCGAISNQLSVLIKQSECPDEFKEAVASLIYAAARFADLPELR 120
           G L E   L   + +++ C  +  QLS + K  ECP++ +EA++SL++AA+ F++LPELR
Sbjct: 61  GLLDELRYLWSLDFVEQTCDFVYKQLSTMQKTPECPEDCREAISSLMFAASGFSELPELR 120

Query: 121 ELRNFFTGKYGCSFEPFTSIELIEKSRATIQTKEIKLQLLQEIAQESAIDWNSKALEQQL 180
           ELR  F  KY  S   F + EL+E   +   + E K++L++++A E +I W+SK  E+++
Sbjct: 121 ELRQMFHEKYTDSLALFVNQELVENMSSKPFSMEKKVKLMEDVALEFSIRWDSKDFEKRI 180


HSP 2 Score: 31.6 bits (70), Expect = 1.9e+00
Identity = 45/161 (27.95%), Postives = 74/161 (45.96%), Query Frame = 0

Query: 236 RKGGSEDEVEDQTPFYLRVIRPPYLKTKPTNKETMEEARKATDQVENDECKKQQPEPTSE 295
           RK    +    ++PFY + + PPY+K     K   +   +  + ++N + +    E    
Sbjct: 502 RKVEERETERMKSPFY-KSLPPPYVK-----KSIAKARHEKAEALDNPKARFDGEEGNHP 561

Query: 296 EKPKPKPKSVRRR-----------NVKLQPTMEIKIDDVGNSGNDMSSSRNKGKEAMKG- 355
           +  K    + RR            N  L+     +   V + G+D  SSR + + + KG 
Sbjct: 562 DNGKNVYGAERRNGAGHHEVNDIDNASLKRQTNRRKHIVESGGDDHISSRRR-ENSRKGL 621

Query: 356 ---EGDDEEEEEEKMLDGLLMHYSKKKPSHESNEVRAVSLS 382
                +DE++ EEKM+D LLMHYSKK  S+E + V+  S S
Sbjct: 622 QVLIDEDEKDSEEKMMDKLLMHYSKKPSSYEKDNVQEESKS 655

BLAST of CmaCh08G006900 vs. TAIR 10
Match: AT4G35730.1 (Regulator of Vps4 activity in the MVB pathway protein )

HSP 1 Score: 107.8 bits (268), Expect = 2.1e-23
Identity = 112/440 (25.45%), Postives = 200/440 (45.45%), Query Frame = 0

Query: 12  TKCKSCLKLLKSRLDAIRKKKNAVLKYLKSDIVELLKSRLDYNAYNRAEGFLVERNVLKC 71
           +KCK+  K+  +R+  IR K+  V+K ++ DI  LL+S  D  A  R E  + E+N+   
Sbjct: 29  SKCKTAAKMAVARIKLIRNKRLVVVKQMRRDIAVLLQSGQDATARIRVEHVIREQNIQAA 88

Query: 72  YELIDEFCGAISNQLSVLIKQSECPDEFKEAVASLIYAAARFADLPELRELRNFFTGKYG 131
            E+I+ FC  I ++L+++ KQ +CP + KE +ASLI+AA R +++PEL +LR+ F  KYG
Sbjct: 89  NEIIELFCELIVSRLTIITKQKQCPVDLKEGIASLIFAAPRCSEIPELGDLRDIFAKKYG 148

Query: 132 CSF-------EPFTSIE--LIEKSRATIQTKEIKLQLLQEIAQESAIDWNSKALEQQLYI 191
             F        P   +   LI+K        E KL++++EIA+E  +DW++   EQ+L +
Sbjct: 149 KDFVSAATDLRPSCGVNRMLIDKLSVRNPGGEYKLKIMKEIAKEFQVDWDTTETEQEL-L 208

Query: 192 PPPNEPDVVPMHERKKDSVGKRDSSDDESMFDIRSEGNTTETSTVDSSTDQDVRKGGSED 251
            P  E    P       S+    ++ +E +   ++   +T + ++++          +  
Sbjct: 209 KPQEESIDGPRKFVSASSLPVNRAAINEPIDPTKAVPRSTSSMSINTHYHDTESAAEAAT 268

Query: 252 EVEDQTPFYLRVIRPPYLKTKPTNKETMEEARKATDQVENDECKKQQPEPTSEE------ 311
           E+  Q     +V      +   +NKE    +  +T Q ++         P S        
Sbjct: 269 ELAKQAVAAAQVASLLATRRDSSNKEFSVSSDHSTHQKDSQYMDHHHHHPGSRRQSRDSE 328

Query: 312 ------KPKPKPKSVRRRNVKLQPTMEIKIDDVGNSGNDMSSSRNKGKEAMKGEGDDEEE 371
                 KP  + + + RR+    P +     +  +   + +++  + KE M+        
Sbjct: 329 TSSYYAKPGAENRGMGRRHSYNNPGI-----NESDYEEEYTNTEAEAKETMRRRHSYNPR 388

Query: 372 EEEKMLDGLLM-----HYSKKKPSHESNEVRAVSLSIDSTEPTELEN--KRHTRTNSFVH 424
                    +      +Y ++    E ++ R  SL  +   P   ++   R   +   VH
Sbjct: 389 SVPPPATSEIKFDESDYYEEETEPDEPSQGRVSSLPPNRAPPQAPQSGESRQDSSGHQVH 448

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q54I396.6e-1427.32IST1-like protein OS=Dictyostelium discoideum OX=44689 GN=DDB_G0289029 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1KJN92.6e-231100.00uncharacterized protein LOC111494616 OS=Cucurbita maxima OX=3661 GN=LOC111494616... [more]
A0A6J1HLZ11.0e-21494.37uncharacterized protein LOC111464187 OS=Cucurbita moschata OX=3662 GN=LOC1114641... [more]
A0A1S3CGT52.8e-14064.73uncharacterized protein LOC103500577 OS=Cucumis melo OX=3656 GN=LOC103500577 PE=... [more]
A0A6J1CWS83.9e-13461.91vacuolar protein sorting-associated protein IST1 OS=Momordica charantia OX=3673 ... [more]
A0A6J1GMM16.6e-13462.39uncharacterized protein LOC111455825 OS=Cucurbita moschata OX=3662 GN=LOC1114558... [more]
Match NameE-valueIdentityDescription
XP_023000359.15.4e-231100.00uncharacterized protein LOC111494616 [Cucurbita maxima][more]
KAG6593450.11.1e-21594.80IST1-like protein, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022964044.12.1e-21494.37uncharacterized protein LOC111464187 [Cucurbita moschata] >KAG7025791.1 IST1-lik... [more]
XP_023514654.13.9e-21394.37uncharacterized protein LOC111778886 [Cucurbita pepo subsp. pepo][more]
XP_008462153.15.7e-14064.73PREDICTED: uncharacterized protein LOC103500577 [Cucumis melo][more]
Match NameE-valueIdentityDescription
AT1G79910.11.2e-5839.50Regulator of Vps4 activity in the MVB pathway protein [more]
AT1G79910.28.2e-3634.90Regulator of Vps4 activity in the MVB pathway protein [more]
AT1G52315.18.5e-3334.34Regulator of Vps4 activity in the MVB pathway protein [more]
AT4G32350.12.5e-3239.44Regulator of Vps4 activity in the MVB pathway protein [more]
AT4G35730.12.1e-2325.45Regulator of Vps4 activity in the MVB pathway protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 266..286
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 217..233
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 265..302
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 365..402
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 234..249
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 190..216
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 181..353
NoneNo IPR availablePANTHERPTHR12161:SF60REGULATOR OF VPS4 ACTIVITY IN THE MVB PATHWAY PROTEINcoord: 9..421
IPR042277Vacuolar protein sorting-associated protein IST1-likeGENE3D1.20.1260.60coord: 3..180
e-value: 5.0E-45
score: 155.3
IPR005061Vacuolar protein sorting-associated protein Ist1PFAMPF03398Ist1coord: 15..169
e-value: 3.1E-39
score: 134.5
IPR005061Vacuolar protein sorting-associated protein Ist1PANTHERPTHR12161IST1 FAMILY MEMBERcoord: 9..421

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh08G006900.1CmaCh08G006900.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0015031 protein transport