CmaCh08G006050 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh08G006050
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
DescriptionDiadenosine tetraphosphate synthetase
LocationCma_Chr08: 3418463 .. 3422478 (+)
RNA-Seq ExpressionCmaCh08G006050
SyntenyCmaCh08G006050
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CTCCACAAACCCTAAAAAAAACCGATAAAGAAGAACTCGTGTGGATTCCAATTCCATTAGAATACAGAAAAAAATATTCGAATGTGGGAATAATGGCCCCACCATCATAAACCTTTTCCCAATTTCTAAACCCTAAATTCTCTCCCTCTCAACCAATTCTGATATCGCCCCCTCGCATGCGTCTTCTATCGTCATTGACTCCACTTGGTCAGCATTTCAGTTTTCGCCTCAGAATTGTCATTTCCTTTCAATCTTGCAATTTCCCCCGTCTGTTTACAATGGCATCTTCCGAGGAATCGCTCAGAAATACCCTTTCTCAAAAGCAATCGGCTGTTGATGCCCAAGCTAATGCTGTTCGTGCCCTCAAGGCTGCTAAGGCTGCCAAGGCTGAGATTGATGCGGCTATTGAAGCTTTGAATGGGTTGAAGATCGAGAAGGCTTCGATTGAGAAGAAGCTGCAGGCGGTTGTTACTGGGGTTGGTGGCGATGGCGCCTTGAACAGGGAGTCATTCCGGCAGGCTGTGAATAACACTCTTGAGAGGCGTTTGTTTTATATTCCATCGTTCAAGATTTACCGTGGGGTTGCTGGATTGTACGATTATGGTCCTCCTGGTTGCGCTGTTAAATCCAATGTTCTTGCCTTTTGGCGCCAGGTTTGTTGATTGAACTTGCTTTTACTTCGATTTTTCGTAATGGGTATCAACCTGAAATTGATTAGAATTGTTCCGGAGTTTGTACTTACTGTATTCAATTCAATTGTGACCAAGTAACTATCTTAGAGTTGTCGTTTTGTTTTTGTTTTTTTTAATCTGTTTGCAGCATTTTGTTCTTGAAGAAAATATGTTGGAAGTTGACTGCCCTTGTGTGACACCGGAAGCTGTCCTGAAAGCATCTGGTCACGTGGATAAGTTCACGGACCTTATGGTCAAGGATGAGAAAACTGGGACCTGCTACAGGGCAGATCACTTGCTTAAGGATTTCTGTAATGAAAAACTTCAGAAAGATCTTAGCATATCCTCTGAGAAGGCTGCAGAATTTAAGCACGTTCTTGCAATCATGGATGATCTCTCTGCTGAACAGCTGGGTGCTAAAATTAAGGAATATGGTATTGTTGCTCCAGATACTAAGAACCCACTGTCTGATCCTTATCCATTCAATTTAATGTTTCAGACATCAATTGGCCCATCAGGCTTGCTCCCTGGGTGAGTATTTGATCCAAATTTACACTTCCATTTGTATAAACTTCATTTCTGAATCAGTATGTTGTAAGAATTTTCTGCTCGAGTTACAACTTCTTGCGATTTAGTTCATCATACTGTTACACAGAAGAGAAAATGGTTGATTTTTTGTTCTCATTGTTATGCCACCTTTTGTTTATCCACTCTCGGTCAATGCAATTCGTTACGTGATTAACTTAGATGTGTATATATGTAAATGATGCAGGTACATGCGCCCCGAAACAGCGCAGGGCATATTTGTCAATTTTAAAGATTTATATTATTACAATGGAAACAAACTGCCCTTTGCTGCAGCACAAATTGGGCAAGCTTTCAGAAATGAGGTTACCCACTTGTTTTTTAGCTTCTTAATTACACTTGCAATTTATGGAGGAGACTTAGCATTACAAGAATGACCTGTATTTTACTTTGTTTTCAATTCCCATGTCTATTATTTTCTGGTTCTTGTGACTGTTGCTTTCAAAATGGTTCTTGTTGCTAGCCCTTGTCCAAGGTTCGTCTGTTTTGTTTCCTTTGGTGTGTATGTTCAATTTTCAGTCCATCTGTTTTACTACAGATATCTCCTCGTCAAGGTCTGCTTAGGGTTCGTGAATTCACGCTAGCAGAGATCGAGCACTTTGTTGATCCTGAAGACAAATCCCATCCAAAATTCACTGATGTAGCGAACTTGGAGTTTTTGATGTTTCCAAGGGAGGAGCAAATGTCTGGTAAATCTGCAAGGATAATTCCCTTGGGTGAAGCTGTTGCAAAGGTTTGTGTACTGAATCTCTACTTCTCATATTCCCACTACCATGTCTCTTGTATCTGTGTGCTAGGTTGATATGATATATTGTGACGCAGGGAACTGTAAATAATGAAACTCTTGGCTACTTCATTGGGAGAGTATATCTTTTCCTAACTCGCCTTGGCATAGACAAAAATCGTTTGCGTTTCCGGCAGCACCTTGCAAATGAAATGGCTCATTATGCAGCTGACTGTTGGGACGCTGAGATTGAATCTTCATATGGTTGGATTGAATGTGTTGGTATTGCTGACAGATCTGCATATGATTTACATGCCCACACGGTACGAATTCAGATAACCTTTATCTTTTCCAAATAGTTGTAATTCAAGATAATGTCTTTAATTTGATGGCAGATATTTCAAAGCTAGTTTAGTTTTCATTGAGTTCCATTAACTGCCCTATTGCTTGGTAGGATTTATAAGATTGAAGTGTCTTCAAGAGTTCTACCATTGAGATTGTGTTTGTATTTGTGAGATCCCACGTCGGTTGGAGAGGGGAACGAAACATTCTTTATAAGGGTGCGGAAACCTCTTCCTAATAGACGCGTTTTAATAACATTGAGTGAAAGCCCAAAGAGGACAATATCTACGAGCGGTGGGCTTAGGCCGTTACAATATTGTTTCAAGTTGCATGTAAATGAACTCTCATATTTTTCACCTCTCCTTTTAATAGGAAAAAAGTGGTGTTCCTCTTGTAGCCCACGAGAAATTCGCGGAACCAAGAGAAGTTGAGGTACTTATCATTCTTGTTATATCTGAAGTTTTTTGAGATCTGTGAATATCTTCATGAGAAAAAAGAACTGTTCTAATGCTCTACATTCACTCGTGATATTAAATTTCAGAAATTGGTCATTGCTCCTGTTAAGAAAGAACTTGGCCTCGCTTTCAAGGGAAGTCAAAAGAACGTTGTGGAAGCTTTGGAGGTAACTTATGGATTTTTTTTTCGGCCGAGCGATTTGTGGCATCTGTAATTTTTGGAAAACCTGACTGTCATAGTATGCAATTATTTTAGGCGATGAAGGAGAAAGAAGCCTTGGAAATGAAAGCAAATCTAGAATCCAATGGAGAGGTGGAGTTCTATGTCTGCACACTGGGGAAGAATGTGTTGATCAAGAAGAGTATGGTTGCAATTTCGAAGGAGAAAAAGAAGGAACATCAGAGGGTTTTCACACCATCCGTGATCGAACCCTCTTTTGGTATTGGTCGGATTATCTATTGCCTCTTTGAACACTCTTACTACATGAGACCAAGTAAAGGTGGGGATGAGCAGCTAAACGTCTTTAGATTTCCACCCCTCGTTGCACCTATCAAGTGCACAGTTTTCCCACTTGTTCAGAATGAGCAGTACGAGCAGGTCGCCAAAGTTATCTCGAAGTCGTTAACTCTATCTGGCATTTCCCATAAAATCGACATTACAGGTACTCGAACATAACTTTCGATTTTGTTTTGCTGTTCTTCTGTAGCTGTAAATGAGACTTTAAGTTCTAAGAAATGAATGAAAACTTGGGAGATGAAAACATAGGATGAATTACAATAATAATGTTTTGCTGTTCTTCCCTGTTTGGTTTAGAATCCTGATCCTTACCTTCTTCTGCATTGATATAACTCTACTCATGTTTTTCGTAATCACTGTCGATTCAGGCACATCGATAGGAAAACGATATGCACGAACAGACGAGCTTGGCGTTCCTTTCGCAATCACTGTCGATTCAACATCTTCAGTGACAATTCGAGAGAGGGATAGCAAAGACCAAATTCGTGTGAGCGTGGAGGAAGCAGCCTCGGTCGTGAAAGATGTGACCGACGGCCTAAGAACATGGGAAAATGTATGGTCTACATTCCCCCATCACGCCTCTGCAGCTGCAGAAGATTGAATTTTTACCTTAAGCAGGTTCTCCAGTTACTTTTTTTTGTAGTTGAGTGCAAGATCCTTATTTTAACCTATAAAAGTATTTACCATGGATAATACTTGAAGATTCTTCTTTAATTTCATTT

mRNA sequence

CTCCACAAACCCTAAAAAAAACCGATAAAGAAGAACTCGTGTGGATTCCAATTCCATTAGAATACAGAAAAAAATATTCGAATGTGGGAATAATGGCCCCACCATCATAAACCTTTTCCCAATTTCTAAACCCTAAATTCTCTCCCTCTCAACCAATTCTGATATCGCCCCCTCGCATGCGTCTTCTATCGTCATTGACTCCACTTGGTCAGCATTTCAGTTTTCGCCTCAGAATTGTCATTTCCTTTCAATCTTGCAATTTCCCCCGTCTGTTTACAATGGCATCTTCCGAGGAATCGCTCAGAAATACCCTTTCTCAAAAGCAATCGGCTGTTGATGCCCAAGCTAATGCTGTTCGTGCCCTCAAGGCTGCTAAGGCTGCCAAGGCTGAGATTGATGCGGCTATTGAAGCTTTGAATGGGTTGAAGATCGAGAAGGCTTCGATTGAGAAGAAGCTGCAGGCGGTTGTTACTGGGGTTGGTGGCGATGGCGCCTTGAACAGGGAGTCATTCCGGCAGGCTGTGAATAACACTCTTGAGAGGCGTTTGTTTTATATTCCATCGTTCAAGATTTACCGTGGGGTTGCTGGATTGTACGATTATGGTCCTCCTGGTTGCGCTGTTAAATCCAATGTTCTTGCCTTTTGGCGCCAGCATTTTGTTCTTGAAGAAAATATGTTGGAAGTTGACTGCCCTTGTGTGACACCGGAAGCTGTCCTGAAAGCATCTGGTCACGTGGATAAGTTCACGGACCTTATGGTCAAGGATGAGAAAACTGGGACCTGCTACAGGGCAGATCACTTGCTTAAGGATTTCTGTAATGAAAAACTTCAGAAAGATCTTAGCATATCCTCTGAGAAGGCTGCAGAATTTAAGCACGTTCTTGCAATCATGGATGATCTCTCTGCTGAACAGCTGGGTGCTAAAATTAAGGAATATGGTATTGTTGCTCCAGATACTAAGAACCCACTGTCTGATCCTTATCCATTCAATTTAATGTTTCAGACATCAATTGGCCCATCAGGCTTGCTCCCTGGGTACATGCGCCCCGAAACAGCGCAGGGCATATTTGTCAATTTTAAAGATTTATATTATTACAATGGAAACAAACTGCCCTTTGCTGCAGCACAAATTGGGCAAGCTTTCAGAAATGAGATATCTCCTCGTCAAGGTCTGCTTAGGGTTCGTGAATTCACGCTAGCAGAGATCGAGCACTTTGTTGATCCTGAAGACAAATCCCATCCAAAATTCACTGATGTAGCGAACTTGGAGTTTTTGATGTTTCCAAGGGAGGAGCAAATGTCTGGTAAATCTGCAAGGATAATTCCCTTGGGTGAAGCTGTTGCAAAGGGAACTGTAAATAATGAAACTCTTGGCTACTTCATTGGGAGAGTATATCTTTTCCTAACTCGCCTTGGCATAGACAAAAATCGTTTGCGTTTCCGGCAGCACCTTGCAAATGAAATGGCTCATTATGCAGCTGACTGTTGGGACGCTGAGATTGAATCTTCATATGGTTGGATTGAATGTGTTGGTATTGCTGACAGATCTGCATATGATTTACATGCCCACACGGAAAAAAGTGGTGTTCCTCTTGTAGCCCACGAGAAATTCGCGGAACCAAGAGAAGTTGAGAAATTGGTCATTGCTCCTGTTAAGAAAGAACTTGGCCTCGCTTTCAAGGGAAGTCAAAAGAACGTTGTGGAAGCTTTGGAGGCGATGAAGGAGAAAGAAGCCTTGGAAATGAAAGCAAATCTAGAATCCAATGGAGAGGTGGAGTTCTATGTCTGCACACTGGGGAAGAATGTGTTGATCAAGAAGAGTATGGTTGCAATTTCGAAGGAGAAAAAGAAGGAACATCAGAGGGTTTTCACACCATCCGTGATCGAACCCTCTTTTGGTATTGGTCGGATTATCTATTGCCTCTTTGAACACTCTTACTACATGAGACCAAGTAAAGGTGGGGATGAGCAGCTAAACGTCTTTAGATTTCCACCCCTCGTTGCACCTATCAAGTGCACAGTTTTCCCACTTGTTCAGAATGAGCAGTACGAGCAGGTCGCCAAAGTTATCTCGAAGTCGTTAACTCTATCTGGCATTTCCCATAAAATCGACATTACAGGCACATCGATAGGAAAACGATATGCACGAACAGACGAGCTTGGCGTTCCTTTCGCAATCACTGTCGATTCAACATCTTCAGTGACAATTCGAGAGAGGGATAGCAAAGACCAAATTCGTGTGAGCGTGGAGGAAGCAGCCTCGGTCGTGAAAGATGTGACCGACGGCCTAAGAACATGGGAAAATGTATGGTCTACATTCCCCCATCACGCCTCTGCAGCTGCAGAAGATTGAATTTTTACCTTAAGCAGGTTCTCCAGTTACTTTTTTTTGTAGTTGAGTGCAAGATCCTTATTTTAACCTATAAAAGTATTTACCATGGATAATACTTGAAGATTCTTCTTTAATTTCATTT

Coding sequence (CDS)

ATGCGTCTTCTATCGTCATTGACTCCACTTGGTCAGCATTTCAGTTTTCGCCTCAGAATTGTCATTTCCTTTCAATCTTGCAATTTCCCCCGTCTGTTTACAATGGCATCTTCCGAGGAATCGCTCAGAAATACCCTTTCTCAAAAGCAATCGGCTGTTGATGCCCAAGCTAATGCTGTTCGTGCCCTCAAGGCTGCTAAGGCTGCCAAGGCTGAGATTGATGCGGCTATTGAAGCTTTGAATGGGTTGAAGATCGAGAAGGCTTCGATTGAGAAGAAGCTGCAGGCGGTTGTTACTGGGGTTGGTGGCGATGGCGCCTTGAACAGGGAGTCATTCCGGCAGGCTGTGAATAACACTCTTGAGAGGCGTTTGTTTTATATTCCATCGTTCAAGATTTACCGTGGGGTTGCTGGATTGTACGATTATGGTCCTCCTGGTTGCGCTGTTAAATCCAATGTTCTTGCCTTTTGGCGCCAGCATTTTGTTCTTGAAGAAAATATGTTGGAAGTTGACTGCCCTTGTGTGACACCGGAAGCTGTCCTGAAAGCATCTGGTCACGTGGATAAGTTCACGGACCTTATGGTCAAGGATGAGAAAACTGGGACCTGCTACAGGGCAGATCACTTGCTTAAGGATTTCTGTAATGAAAAACTTCAGAAAGATCTTAGCATATCCTCTGAGAAGGCTGCAGAATTTAAGCACGTTCTTGCAATCATGGATGATCTCTCTGCTGAACAGCTGGGTGCTAAAATTAAGGAATATGGTATTGTTGCTCCAGATACTAAGAACCCACTGTCTGATCCTTATCCATTCAATTTAATGTTTCAGACATCAATTGGCCCATCAGGCTTGCTCCCTGGGTACATGCGCCCCGAAACAGCGCAGGGCATATTTGTCAATTTTAAAGATTTATATTATTACAATGGAAACAAACTGCCCTTTGCTGCAGCACAAATTGGGCAAGCTTTCAGAAATGAGATATCTCCTCGTCAAGGTCTGCTTAGGGTTCGTGAATTCACGCTAGCAGAGATCGAGCACTTTGTTGATCCTGAAGACAAATCCCATCCAAAATTCACTGATGTAGCGAACTTGGAGTTTTTGATGTTTCCAAGGGAGGAGCAAATGTCTGGTAAATCTGCAAGGATAATTCCCTTGGGTGAAGCTGTTGCAAAGGGAACTGTAAATAATGAAACTCTTGGCTACTTCATTGGGAGAGTATATCTTTTCCTAACTCGCCTTGGCATAGACAAAAATCGTTTGCGTTTCCGGCAGCACCTTGCAAATGAAATGGCTCATTATGCAGCTGACTGTTGGGACGCTGAGATTGAATCTTCATATGGTTGGATTGAATGTGTTGGTATTGCTGACAGATCTGCATATGATTTACATGCCCACACGGAAAAAAGTGGTGTTCCTCTTGTAGCCCACGAGAAATTCGCGGAACCAAGAGAAGTTGAGAAATTGGTCATTGCTCCTGTTAAGAAAGAACTTGGCCTCGCTTTCAAGGGAAGTCAAAAGAACGTTGTGGAAGCTTTGGAGGCGATGAAGGAGAAAGAAGCCTTGGAAATGAAAGCAAATCTAGAATCCAATGGAGAGGTGGAGTTCTATGTCTGCACACTGGGGAAGAATGTGTTGATCAAGAAGAGTATGGTTGCAATTTCGAAGGAGAAAAAGAAGGAACATCAGAGGGTTTTCACACCATCCGTGATCGAACCCTCTTTTGGTATTGGTCGGATTATCTATTGCCTCTTTGAACACTCTTACTACATGAGACCAAGTAAAGGTGGGGATGAGCAGCTAAACGTCTTTAGATTTCCACCCCTCGTTGCACCTATCAAGTGCACAGTTTTCCCACTTGTTCAGAATGAGCAGTACGAGCAGGTCGCCAAAGTTATCTCGAAGTCGTTAACTCTATCTGGCATTTCCCATAAAATCGACATTACAGGCACATCGATAGGAAAACGATATGCACGAACAGACGAGCTTGGCGTTCCTTTCGCAATCACTGTCGATTCAACATCTTCAGTGACAATTCGAGAGAGGGATAGCAAAGACCAAATTCGTGTGAGCGTGGAGGAAGCAGCCTCGGTCGTGAAAGATGTGACCGACGGCCTAAGAACATGGGAAAATGTATGGTCTACATTCCCCCATCACGCCTCTGCAGCTGCAGAAGATTGA

Protein sequence

MRLLSSLTPLGQHFSFRLRIVISFQSCNFPRLFTMASSEESLRNTLSQKQSAVDAQANAVRALKAAKAAKAEIDAAIEALNGLKIEKASIEKKLQAVVTGVGGDGALNRESFRQAVNNTLERRLFYIPSFKIYRGVAGLYDYGPPGCAVKSNVLAFWRQHFVLEENMLEVDCPCVTPEAVLKASGHVDKFTDLMVKDEKTGTCYRADHLLKDFCNEKLQKDLSISSEKAAEFKHVLAIMDDLSAEQLGAKIKEYGIVAPDTKNPLSDPYPFNLMFQTSIGPSGLLPGYMRPETAQGIFVNFKDLYYYNGNKLPFAAAQIGQAFRNEISPRQGLLRVREFTLAEIEHFVDPEDKSHPKFTDVANLEFLMFPREEQMSGKSARIIPLGEAVAKGTVNNETLGYFIGRVYLFLTRLGIDKNRLRFRQHLANEMAHYAADCWDAEIESSYGWIECVGIADRSAYDLHAHTEKSGVPLVAHEKFAEPREVEKLVIAPVKKELGLAFKGSQKNVVEALEAMKEKEALEMKANLESNGEVEFYVCTLGKNVLIKKSMVAISKEKKKEHQRVFTPSVIEPSFGIGRIIYCLFEHSYYMRPSKGGDEQLNVFRFPPLVAPIKCTVFPLVQNEQYEQVAKVISKSLTLSGISHKIDITGTSIGKRYARTDELGVPFAITVDSTSSVTIRERDSKDQIRVSVEEAASVVKDVTDGLRTWENVWSTFPHHASAAAED
Homology
BLAST of CmaCh08G006050 vs. ExPASy Swiss-Prot
Match: O23627 (Glycine--tRNA ligase, mitochondrial 1 OS=Arabidopsis thaliana OX=3702 GN=At1g29880 PE=1 SV=1)

HSP 1 Score: 1177.5 bits (3045), Expect = 0.0e+00
Identity = 578/696 (83.05%), Postives = 646/696 (92.82%), Query Frame = 0

Query: 30  PRLFTMASSEESLRNTLSQKQSAVDAQANAVRALKAAKAAKAEIDAAIEALNGLKIEKAS 89
           P    M ++E+SLR +LS+K S+V+AQ NAVRALKA++AAK EIDAAIE LN LK+EK++
Sbjct: 35  PIQIPMDATEQSLRQSLSEKSSSVEAQGNAVRALKASRAAKPEIDAAIEQLNKLKLEKST 94

Query: 90  IEKKLQAVVTGVGGDGALNRESFRQAVNNTLERRLFYIPSFKIYRGVAGLYDYGPPGCAV 149
           +EK+LQ++++   G+G+LNRE+FR+AV NTLERRLFYIPSFKIY GVAGL+DYGPPGCA+
Sbjct: 95  VEKELQSIISS-SGNGSLNREAFRKAVVNTLERRLFYIPSFKIYSGVAGLFDYGPPGCAI 154

Query: 150 KSNVLAFWRQHFVLEENMLEVDCPCVTPEAVLKASGHVDKFTDLMVKDEKTGTCYRADHL 209
           KSNVL+FWRQHF+LEENMLEVDCPCVTPE VLKASGHVDKFTDLMVKDEKTGTCYRADHL
Sbjct: 155 KSNVLSFWRQHFILEENMLEVDCPCVTPEVVLKASGHVDKFTDLMVKDEKTGTCYRADHL 214

Query: 210 LKDFCNEKLQKDLSISSEKAAEFKHVLAIMDDLSAEQLGAKIKEYGIVAPDTKNPLSDPY 269
           LKD+C EKL+KDL+IS+EKAAE K VLA+M+D S EQLGAKI+EYGI APDTKNPLSDPY
Sbjct: 215 LKDYCTEKLEKDLTISAEKAAELKDVLAVMEDFSPEQLGAKIREYGITAPDTKNPLSDPY 274

Query: 270 PFNLMFQTSIGPSGLLPGYMRPETAQGIFVNFKDLYYYNGNKLPFAAAQIGQAFRNEISP 329
           PFNLMFQTSIGPSGL+PGYMRPETAQGIFVNFKDLYYYNG KLPFAAAQIGQAFRNEISP
Sbjct: 275 PFNLMFQTSIGPSGLIPGYMRPETAQGIFVNFKDLYYYNGKKLPFAAAQIGQAFRNEISP 334

Query: 330 RQGLLRVREFTLAEIEHFVDPEDKSHPKFTDVANLEFLMFPREEQMSGKSARIIPLGEAV 389
           RQGLLRVREFTLAEIEHFVDPE+KSHPKF+DVA LEFLMFPREEQMSG+SA+ + LGEAV
Sbjct: 335 RQGLLRVREFTLAEIEHFVDPENKSHPKFSDVAKLEFLMFPREEQMSGQSAKKLCLGEAV 394

Query: 390 AKGTVNNETLGYFIGRVYLFLTRLGIDKNRLRFRQHLANEMAHYAADCWDAEIESSYGWI 449
           AKGTVNNETLGYFIGRVYLFLTRLGIDK RLRFRQHLANEMAHYAADCWDAEIESSYGWI
Sbjct: 395 AKGTVNNETLGYFIGRVYLFLTRLGIDKERLRFRQHLANEMAHYAADCWDAEIESSYGWI 454

Query: 450 ECVGIADRSAYDLHAHTEKSGVPLVAHEKFAEPREVEKLVIAPVKKELGLAFKGSQKNVV 509
           ECVGIADRSAYDL AH++KSG PLVA EKFAEP+EVEKLVI PVKKELGLAFKG+QKNVV
Sbjct: 455 ECVGIADRSAYDLRAHSDKSGTPLVAEEKFAEPKEVEKLVITPVKKELGLAFKGNQKNVV 514

Query: 510 EALEAMKEKEALEMKANLESNGEVEFYVCTLGKNVLIKKSMVAISKEKKKEHQRVFTPSV 569
           E+LEAM E+EA+EMKA LES GEVEFYVCTL K+V IKK+MV+ISKEKKKEHQRVFTPSV
Sbjct: 515 ESLEAMNEEEAMEMKATLESKGEVEFYVCTLKKSVNIKKNMVSISKEKKKEHQRVFTPSV 574

Query: 570 IEPSFGIGRIIYCLFEHSYYMRPSKGGDEQLNVFRFPPLVAPIKCTVFPLVQNEQYEQVA 629
           IEPSFGIGRIIYCL+EH +  RPSK GDEQLN+FRFPPLVAPIKCTVFPLVQN+Q+E+VA
Sbjct: 575 IEPSFGIGRIIYCLYEHCFSTRPSKAGDEQLNLFRFPPLVAPIKCTVFPLVQNQQFEEVA 634

Query: 630 KVISKSLTLSGISHKIDITGTSIGKRYARTDELGVPFAITVDSTSSVTIRERDSKDQIRV 689
           KVISK L   GISHKIDITGTSIGKRYARTDELGVPFAITVDS +SVTIRERDSKDQ+RV
Sbjct: 635 KVISKELASVGISHKIDITGTSIGKRYARTDELGVPFAITVDSDTSVTIRERDSKDQVRV 694

Query: 690 SVEEAASVVKDVTDGLRTWENVWSTFPHHASAAAED 726
           +++EAASVV  V++G  TW++VW+TFPHH+SAAA++
Sbjct: 695 TLKEAASVVSSVSEGKMTWQDVWATFPHHSSAAADE 729

BLAST of CmaCh08G006050 vs. ExPASy Swiss-Prot
Match: Q5I0G4 (Glycine--tRNA ligase OS=Rattus norvegicus OX=10116 GN=Gars1 PE=1 SV=2)

HSP 1 Score: 655.6 bits (1690), Expect = 6.5e-187
Identity = 345/686 (50.29%), Postives = 456/686 (66.47%), Query Frame = 0

Query: 37  SSEESLRNTLSQKQSAVDAQANAVRALKAAKAAKAEIDAAIEALNGLKIEKASIEKKLQA 96
           SS +S    L+  + AV  Q + VR LK  KA + ++D A+     LK  K  +E K  A
Sbjct: 44  SSMDSAEELLAPLRLAVRQQGDFVRKLKEDKAPQVDVDRAVAE---LKARKRVLEAKELA 103

Query: 97  VVTGVGGDGALNRESFRQAVNNTLERRLFYIPSFKIYRGVAGLYDYGPPGCAVKSNVLAF 156
           +      D  ++R      + +TL+RR FY  +F IY GV+GLYD+GP GCA+K+N++  
Sbjct: 104 LQP---KDDIVDRAK----MEDTLKRRFFYDQAFAIYGGVSGLYDFGPVGCALKNNIIQT 163

Query: 157 WRQHFVLEENMLEVDCPCVTPEAVLKASGHVDKFTDLMVKDEKTGTCYRADHLLKDFCNE 216
           WRQHF+ EE +LE+DC  +TPE VLK SGHVDKF D MVKD K G C+RADHLLK    +
Sbjct: 164 WRQHFIQEEQILEIDCTMLTPEPVLKTSGHVDKFADFMVKDVKNGECFRADHLLKAHL-Q 223

Query: 217 KLQKDLSISSEKAAEFKHVLAIMDDLSAEQLGAKIKEYGIVAPDTKNPLSDPYPFNLMFQ 276
           KL  D   S+EK +E + VLA +D+   ++L      Y + +P T N LS P PFNLMFQ
Sbjct: 224 KLMSDKKCSAEKKSEMESVLAQLDNYGQQELADLFVNYNVKSPTTGNDLSPPVPFNLMFQ 283

Query: 277 TSIGPSGLLPGYMRPETAQGIFVNFKDLYYYNGNKLPFAAAQIGQAFRNEISPRQGLLRV 336
           T IGP G +PGY+RPETAQGIF+NFK L  +N  KLPFAAAQIG +FRNEISPR GL+RV
Sbjct: 284 TFIGPGGNMPGYLRPETAQGIFLNFKRLLEFNQGKLPFAAAQIGNSFRNEISPRSGLIRV 343

Query: 337 REFTLAEIEHFVDPEDKSHPKFTDVANLEFLMFPREEQMSGKSARIIPLGEAVAKGTVNN 396
           REFT+AEIEHFVDP +K HPKF  VA+L   ++  + Q++G+SAR + LG+AV +G +NN
Sbjct: 344 REFTMAEIEHFVDPTEKDHPKFPSVADLYLYLYSAKAQVTGQSARKMRLGDAVEQGVINN 403

Query: 397 ETLGYFIGRVYLFLTRLGIDKNRLRFRQHLANEMAHYAADCWDAEIESSYGWIECVGIAD 456
             LGYFIGR+YL+LT++GI  ++LRFRQH+ NEMAHYA DCWDAE ++SYGWIE VG AD
Sbjct: 404 SVLGYFIGRIYLYLTKVGISPDKLRFRQHMENEMAHYACDCWDAESKTSYGWIEIVGCAD 463

Query: 457 RSAYDLHAHTEKSGVPLVAHEKFAEPREVEKLVIAPVKKELGLAFKGSQKNVVEALEAMK 516
           RS YDL  H   + VPLVA +   EP+ V  +   P K  +G A+K   K V+E L A  
Sbjct: 464 RSCYDLSCHARATKVPLVAEKPLKEPKTVNVVQFEPNKGAVGKAYKKDAKLVLEYLGACD 523

Query: 517 EKEALEMKANLESNGEVEFYVCTLGKNVLIKKSMVAISKEKKKEHQRVFTPSVIEPSFGI 576
           E    EM+  L   G  EF + T GK   + K MV++ + +K  H     PSVIEPSFG+
Sbjct: 524 ECYITEMELLLSEKG--EFTIETEGKTFQLTKDMVSVKRFQKTLHVEEVVPSVIEPSFGL 583

Query: 577 GRIIYCLFEHSYYMRPSKGGDEQLNVFRFPPLVAPIKCTVFPLVQNEQYEQVAKVISKSL 636
           GRI+Y + EH++++R    GDEQ   F FP +VAP KC+V PL QN+++    K +S++L
Sbjct: 584 GRIMYTILEHTFHVRE---GDEQRTFFSFPAVVAPFKCSVLPLSQNQEFMPFVKELSEAL 643

Query: 637 TLSGISHKIDITGTSIGKRYARTDELGVPFAITVD------STSSVTIRERDSKDQIRVS 696
           T +G+SHK+D +  SIG+RYARTDE+GV F IT+D      +  + T+R+RDS  QIR  
Sbjct: 644 TRNGVSHKVDDSSGSIGRRYARTDEIGVAFGITIDFDTVNKTPHTATLRDRDSMRQIRAE 703

Query: 697 VEEAASVVKDVTDGLRTWENVWSTFP 717
           V E  SVV+D+ +G  TW +V + +P
Sbjct: 704 VSELPSVVRDLANGNITWADVEARYP 713

BLAST of CmaCh08G006050 vs. ExPASy Swiss-Prot
Match: Q9CZD3 (Glycine--tRNA ligase OS=Mus musculus OX=10090 GN=Gars1 PE=1 SV=1)

HSP 1 Score: 654.1 bits (1686), Expect = 1.9e-186
Identity = 344/686 (50.15%), Postives = 456/686 (66.47%), Query Frame = 0

Query: 37  SSEESLRNTLSQKQSAVDAQANAVRALKAAKAAKAEIDAAIEALNGLKIEKASIEKKLQA 96
           SS +S    L+  + AV  Q + VR LK  KA + ++D A+     LK  K  +E K  A
Sbjct: 45  SSMDSAEELLAPLRLAVRQQGDFVRKLKEDKAPQVDVDRAVAE---LKARKRVLEAKELA 104

Query: 97  VVTGVGGDGALNRESFRQAVNNTLERRLFYIPSFKIYRGVAGLYDYGPPGCAVKSNVLAF 156
           +      D  ++R      + +TL+RR FY  +F IY GV+GLYD+GP GCA+K+N++  
Sbjct: 105 LQP---KDDIVDRAK----MEDTLKRRFFYDQAFAIYGGVSGLYDFGPVGCALKNNIIQA 164

Query: 157 WRQHFVLEENMLEVDCPCVTPEAVLKASGHVDKFTDLMVKDEKTGTCYRADHLLKDFCNE 216
           WRQHF+ EE +LE+DC  +TPE VLK SGHVDKF D MVKD K G C+RADHLLK    +
Sbjct: 165 WRQHFIQEEQILEIDCTMLTPEPVLKTSGHVDKFADFMVKDVKNGECFRADHLLKAHL-Q 224

Query: 217 KLQKDLSISSEKAAEFKHVLAIMDDLSAEQLGAKIKEYGIVAPDTKNPLSDPYPFNLMFQ 276
           KL  D   S+EK +E + VLA +D+   ++L      Y + +P T N LS P PFNLMFQ
Sbjct: 225 KLMSDKKCSAEKKSEMESVLAQLDNYGQQELADLFVNYNVKSPTTGNDLSPPVPFNLMFQ 284

Query: 277 TSIGPSGLLPGYMRPETAQGIFVNFKDLYYYNGNKLPFAAAQIGQAFRNEISPRQGLLRV 336
           T IGP G +PGY+RPETAQGIF+NFK L  +N  KLPFAAAQIG +FRNEISPR GL+RV
Sbjct: 285 TFIGPGGNMPGYLRPETAQGIFLNFKRLLEFNQGKLPFAAAQIGNSFRNEISPRSGLIRV 344

Query: 337 REFTLAEIEHFVDPEDKSHPKFTDVANLEFLMFPREEQMSGKSARIIPLGEAVAKGTVNN 396
           REFT+AEIEHFVDP +K HPKF  VA+L   ++  + Q++G+SAR + LG+AV +G +NN
Sbjct: 345 REFTMAEIEHFVDPTEKDHPKFQSVADLCLYLYSAKAQVTGQSARKMRLGDAVEQGVINN 404

Query: 397 ETLGYFIGRVYLFLTRLGIDKNRLRFRQHLANEMAHYAADCWDAEIESSYGWIECVGIAD 456
             LGYFIGR+YL+LT++GI  ++LRFRQH+ NEMAHYA DCWDAE ++SYGWIE VG AD
Sbjct: 405 SVLGYFIGRIYLYLTKVGISPDKLRFRQHMENEMAHYACDCWDAESKTSYGWIEIVGCAD 464

Query: 457 RSAYDLHAHTEKSGVPLVAHEKFAEPREVEKLVIAPVKKELGLAFKGSQKNVVEALEAMK 516
           RS YDL  H   + VPLVA +   EP+ V  +   P K  +G A+K   K V+E L A  
Sbjct: 465 RSCYDLSCHARATKVPLVAEKPLKEPKTVNVVQFEPNKGAVGKAYKKDAKLVLEYLSACD 524

Query: 517 EKEALEMKANLESNGEVEFYVCTLGKNVLIKKSMVAISKEKKKEHQRVFTPSVIEPSFGI 576
           E    EM+  L   G  EF + T GK   + K MV++ + +K  H     PSVIEPSFG+
Sbjct: 525 ECYISEMELLLSEKG--EFTIETEGKTFQLTKDMVSVKRFQKTLHVEEVVPSVIEPSFGL 584

Query: 577 GRIIYCLFEHSYYMRPSKGGDEQLNVFRFPPLVAPIKCTVFPLVQNEQYEQVAKVISKSL 636
           GRI+Y + EH++++R    GDEQ   F FP +VAP KC+V PL QN+++    K +S++L
Sbjct: 585 GRIMYTILEHTFHVRE---GDEQRTFFSFPAVVAPFKCSVLPLSQNQEFMPFVKELSEAL 644

Query: 637 TLSGISHKIDITGTSIGKRYARTDELGVPFAITVD------STSSVTIRERDSKDQIRVS 696
           T +G+SHK+D +  SIG+RYARTDE+GV F IT+D      +  + T+R+RDS  QIR  
Sbjct: 645 TRNGVSHKVDDSSGSIGRRYARTDEIGVAFGITIDFDTVNKTPHTATLRDRDSMRQIRAE 704

Query: 697 VEEAASVVKDVTDGLRTWENVWSTFP 717
           V E  +VV+D+ +G  TW +V + +P
Sbjct: 705 VSELPNVVRDLANGNITWADVEARYP 714

BLAST of CmaCh08G006050 vs. ExPASy Swiss-Prot
Match: Q5RBL1 (Glycine--tRNA ligase OS=Pongo abelii OX=9601 GN=GARS1 PE=2 SV=1)

HSP 1 Score: 644.0 bits (1660), Expect = 2.0e-183
Identity = 347/713 (48.67%), Postives = 462/713 (64.80%), Query Frame = 0

Query: 15  SFRLRIVISFQSCNFPRLFTMASSEESL-----RNTLSQKQSAVDAQANAVRALKAAKAA 74
           S  LR  +S  SC  P     A+S  S+        L+  + AV  Q + VR LK  KA 
Sbjct: 29  SLLLRRPLSAPSC-APISLPAAASRSSVDGAGAEEVLAPLRLAVRQQGDLVRKLKEDKAP 88

Query: 75  KAEIDAAIEALNGLKIEKASIEKKLQAVVTGVGGDGALNRESFRQAVNNTLERRLFYIPS 134
           + ++D A+     LK  K  +E K  A+      D  ++R      + +TL+RR FY  +
Sbjct: 89  QVDVDKAVAE---LKARKRVLEAKELALQP---KDDIVDRAK----MEDTLKRRFFYDQA 148

Query: 135 FKIYRGVAGLYDYGPPGCAVKSNVLAFWRQHFVLEENMLEVDCPCVTPEAVLKASGHVDK 194
           F IY GV+GLYD+GP GCA+K+N++  WRQHF+ EE +LE+DC  +TPE VLK SGHVDK
Sbjct: 149 FAIYGGVSGLYDFGPVGCALKNNIIQTWRQHFIQEEQILEIDCTMLTPEPVLKTSGHVDK 208

Query: 195 FTDLMVKDEKTGTCYRADHLLKDFCNEKLQKDLSISSEKAAEFKHVLAIMDDLSAEQLGA 254
           F D MVKD K G C+RADHLLK    +KL  D   S EK +E + VLA +D+   ++L  
Sbjct: 209 FADFMVKDVKNGECFRADHLLKAHL-QKLMSDKKCSVEKKSEMESVLAQLDNYGQQELAD 268

Query: 255 KIKEYGIVAPDTKNPLSDPYPFNLMFQTSIGPSGLLPGYMRPETAQGIFVNFKDLYYYNG 314
               Y + +P T N LS P  FNLMF+T IGP G +PGY+RPETAQGIF+NFK L  +N 
Sbjct: 269 LFVNYNVKSPITGNDLSPPVSFNLMFKTFIGPGGNMPGYLRPETAQGIFLNFKRLLEFNQ 328

Query: 315 NKLPFAAAQIGQAFRNEISPRQGLLRVREFTLAEIEHFVDPEDKSHPKFTDVANLEFLMF 374
            KLPFAAAQIG +FRNEISPR GL+RVREFT+AEIEHFVDP +K HPKF +VA+L   ++
Sbjct: 329 GKLPFAAAQIGNSFRNEISPRSGLIRVREFTMAEIEHFVDPSEKDHPKFQNVADLHLYLY 388

Query: 375 PREEQMSGKSARIIPLGEAVAKGTVNNETLGYFIGRVYLFLTRLGIDKNRLRFRQHLANE 434
             + Q+SG+SAR + LG+AV +G +NN  LGYFIGR+YL+LT++GI  ++LRFRQH+ NE
Sbjct: 389 SAKAQVSGQSARKMRLGDAVEQGVINNTVLGYFIGRIYLYLTKVGISPDKLRFRQHMENE 448

Query: 435 MAHYAADCWDAEIESSYGWIECVGIADRSAYDLHAHTEKSGVPLVAHEKFAEPREVEKLV 494
           MAHYA DCWDAE ++SYGWIE VG ADRS YDL  H   + VPLVA +   EP+ V  + 
Sbjct: 449 MAHYACDCWDAESKTSYGWIEIVGCADRSCYDLSCHARATKVPLVAEKPLKEPKTVNVVQ 508

Query: 495 IAPVKKELGLAFKGSQKNVVEALEAMKEKEALEMKANLESNGEVEFYVCTLGKNVLIKKS 554
             P K  +G A+K   K V+E L    E    EM+  L   G  EF + T GK   + K 
Sbjct: 509 FEPNKGAIGKAYKKDAKLVMEYLAICDECYITEMEMLLNEKG--EFTIETEGKTFQLTKD 568

Query: 555 MVAISKEKKKEHQRVFTPSVIEPSFGIGRIIYCLFEHSYYMRPSKGGDEQLNVFRFPPLV 614
           MV + + +K  +     P+VIEPSFG+GRI+Y +FEH++++R    GDEQ   F FP +V
Sbjct: 569 MVNVKRFQKTLYVEEVVPNVIEPSFGLGRIMYTVFEHTFHVRE---GDEQRTFFSFPAVV 628

Query: 615 APIKCTVFPLVQNEQYEQVAKVISKSLTLSGISHKIDITGTSIGKRYARTDELGVPFAIT 674
           AP KC+V PL QN+++    K +S++LT  G+SHK+D +  SIG+RYARTDE+GV F +T
Sbjct: 629 APFKCSVLPLSQNQEFMPFVKELSEALTRHGVSHKVDDSSGSIGRRYARTDEIGVAFGVT 688

Query: 675 VD------STSSVTIRERDSKDQIRVSVEEAASVVKDVTDGLRTWENVWSTFP 717
           +D      +  + T+R+RDS  QIR  V E  S+V+D+ +G  TW +V + +P
Sbjct: 689 IDFDTVNKTPHTATLRDRDSMRQIRAEVSELPSIVRDLANGNITWADVEARYP 724

BLAST of CmaCh08G006050 vs. ExPASy Swiss-Prot
Match: P41250 (Glycine--tRNA ligase OS=Homo sapiens OX=9606 GN=GARS1 PE=1 SV=3)

HSP 1 Score: 643.7 bits (1659), Expect = 2.6e-183
Identity = 345/713 (48.39%), Postives = 462/713 (64.80%), Query Frame = 0

Query: 15  SFRLRIVISFQSCNFPRLFTMASSEESL-----RNTLSQKQSAVDAQANAVRALKAAKAA 74
           S  LR  +S  SC  P     A+S  S+        L+  + AV  Q + VR LK  KA 
Sbjct: 29  SLLLRRSLSAASCP-PISLPAAASRSSMDGAGAEEVLAPLRLAVRQQGDLVRKLKEDKAP 88

Query: 75  KAEIDAAIEALNGLKIEKASIEKKLQAVVTGVGGDGALNRESFRQAVNNTLERRLFYIPS 134
           + ++D A+     LK  K  +E K  A+      D  ++R      + +TL+RR FY  +
Sbjct: 89  QVDVDKAVAE---LKARKRVLEAKELALQP---KDDIVDRAK----MEDTLKRRFFYDQA 148

Query: 135 FKIYRGVAGLYDYGPPGCAVKSNVLAFWRQHFVLEENMLEVDCPCVTPEAVLKASGHVDK 194
           F IY GV+GLYD+GP GCA+K+N++  WRQHF+ EE +LE+DC  +TPE VLK SGHVDK
Sbjct: 149 FAIYGGVSGLYDFGPVGCALKNNIIQTWRQHFIQEEQILEIDCTMLTPEPVLKTSGHVDK 208

Query: 195 FTDLMVKDEKTGTCYRADHLLKDFCNEKLQKDLSISSEKAAEFKHVLAIMDDLSAEQLGA 254
           F D MVKD K G C+RADHLLK    +KL  D   S EK +E + VLA +D+   ++L  
Sbjct: 209 FADFMVKDVKNGECFRADHLLKAHL-QKLMSDKKCSVEKKSEMESVLAQLDNYGQQELAD 268

Query: 255 KIKEYGIVAPDTKNPLSDPYPFNLMFQTSIGPSGLLPGYMRPETAQGIFVNFKDLYYYNG 314
               Y + +P T N LS P  FNLMF+T IGP G +PGY+RPETAQGIF+NFK L  +N 
Sbjct: 269 LFVNYNVKSPITGNDLSPPVSFNLMFKTFIGPGGNMPGYLRPETAQGIFLNFKRLLEFNQ 328

Query: 315 NKLPFAAAQIGQAFRNEISPRQGLLRVREFTLAEIEHFVDPEDKSHPKFTDVANLEFLMF 374
            KLPFAAAQIG +FRNEISPR GL+RVREFT+AEIEHFVDP +K HPKF +VA+L   ++
Sbjct: 329 GKLPFAAAQIGNSFRNEISPRSGLIRVREFTMAEIEHFVDPSEKDHPKFQNVADLHLYLY 388

Query: 375 PREEQMSGKSARIIPLGEAVAKGTVNNETLGYFIGRVYLFLTRLGIDKNRLRFRQHLANE 434
             + Q+SG+SAR + LG+AV +G +NN  LGYFIGR+YL+LT++GI  ++LRFRQH+ NE
Sbjct: 389 SAKAQVSGQSARKMRLGDAVEQGVINNTVLGYFIGRIYLYLTKVGISPDKLRFRQHMENE 448

Query: 435 MAHYAADCWDAEIESSYGWIECVGIADRSAYDLHAHTEKSGVPLVAHEKFAEPREVEKLV 494
           MAHYA DCWDAE ++SYGWIE VG ADRS YDL  H   + VPLVA +   EP+ V  + 
Sbjct: 449 MAHYACDCWDAESKTSYGWIEIVGCADRSCYDLSCHARATKVPLVAEKPLKEPKTVNVVQ 508

Query: 495 IAPVKKELGLAFKGSQKNVVEALEAMKEKEALEMKANLESNGEVEFYVCTLGKNVLIKKS 554
             P K  +G A+K   K V+E L    E    EM+  L   G  EF + T GK   + K 
Sbjct: 509 FEPSKGAIGKAYKKDAKLVMEYLAICDECYITEMEMLLNEKG--EFTIETEGKTFQLTKD 568

Query: 555 MVAISKEKKKEHQRVFTPSVIEPSFGIGRIIYCLFEHSYYMRPSKGGDEQLNVFRFPPLV 614
           M+ + + +K  +     P+VIEPSFG+GRI+Y +FEH++++R    GDEQ   F FP +V
Sbjct: 569 MINVKRFQKTLYVEEVVPNVIEPSFGLGRIMYTVFEHTFHVRE---GDEQRTFFSFPAVV 628

Query: 615 APIKCTVFPLVQNEQYEQVAKVISKSLTLSGISHKIDITGTSIGKRYARTDELGVPFAIT 674
           AP KC+V PL QN+++    K +S++LT  G+SHK+D +  SIG+RYARTDE+GV F +T
Sbjct: 629 APFKCSVLPLSQNQEFMPFVKELSEALTRHGVSHKVDDSSGSIGRRYARTDEIGVAFGVT 688

Query: 675 VD------STSSVTIRERDSKDQIRVSVEEAASVVKDVTDGLRTWENVWSTFP 717
           +D      +  + T+R+RDS  QIR  + E  S+V+D+ +G  TW +V + +P
Sbjct: 689 IDFDTVNKTPHTATLRDRDSMRQIRAEISELPSIVQDLANGNITWADVEARYP 724

BLAST of CmaCh08G006050 vs. TAIR 10
Match: AT1G29880.1 (glycyl-tRNA synthetase / glycine--tRNA ligase )

HSP 1 Score: 1177.5 bits (3045), Expect = 0.0e+00
Identity = 578/696 (83.05%), Postives = 646/696 (92.82%), Query Frame = 0

Query: 30  PRLFTMASSEESLRNTLSQKQSAVDAQANAVRALKAAKAAKAEIDAAIEALNGLKIEKAS 89
           P    M ++E+SLR +LS+K S+V+AQ NAVRALKA++AAK EIDAAIE LN LK+EK++
Sbjct: 35  PIQIPMDATEQSLRQSLSEKSSSVEAQGNAVRALKASRAAKPEIDAAIEQLNKLKLEKST 94

Query: 90  IEKKLQAVVTGVGGDGALNRESFRQAVNNTLERRLFYIPSFKIYRGVAGLYDYGPPGCAV 149
           +EK+LQ++++   G+G+LNRE+FR+AV NTLERRLFYIPSFKIY GVAGL+DYGPPGCA+
Sbjct: 95  VEKELQSIISS-SGNGSLNREAFRKAVVNTLERRLFYIPSFKIYSGVAGLFDYGPPGCAI 154

Query: 150 KSNVLAFWRQHFVLEENMLEVDCPCVTPEAVLKASGHVDKFTDLMVKDEKTGTCYRADHL 209
           KSNVL+FWRQHF+LEENMLEVDCPCVTPE VLKASGHVDKFTDLMVKDEKTGTCYRADHL
Sbjct: 155 KSNVLSFWRQHFILEENMLEVDCPCVTPEVVLKASGHVDKFTDLMVKDEKTGTCYRADHL 214

Query: 210 LKDFCNEKLQKDLSISSEKAAEFKHVLAIMDDLSAEQLGAKIKEYGIVAPDTKNPLSDPY 269
           LKD+C EKL+KDL+IS+EKAAE K VLA+M+D S EQLGAKI+EYGI APDTKNPLSDPY
Sbjct: 215 LKDYCTEKLEKDLTISAEKAAELKDVLAVMEDFSPEQLGAKIREYGITAPDTKNPLSDPY 274

Query: 270 PFNLMFQTSIGPSGLLPGYMRPETAQGIFVNFKDLYYYNGNKLPFAAAQIGQAFRNEISP 329
           PFNLMFQTSIGPSGL+PGYMRPETAQGIFVNFKDLYYYNG KLPFAAAQIGQAFRNEISP
Sbjct: 275 PFNLMFQTSIGPSGLIPGYMRPETAQGIFVNFKDLYYYNGKKLPFAAAQIGQAFRNEISP 334

Query: 330 RQGLLRVREFTLAEIEHFVDPEDKSHPKFTDVANLEFLMFPREEQMSGKSARIIPLGEAV 389
           RQGLLRVREFTLAEIEHFVDPE+KSHPKF+DVA LEFLMFPREEQMSG+SA+ + LGEAV
Sbjct: 335 RQGLLRVREFTLAEIEHFVDPENKSHPKFSDVAKLEFLMFPREEQMSGQSAKKLCLGEAV 394

Query: 390 AKGTVNNETLGYFIGRVYLFLTRLGIDKNRLRFRQHLANEMAHYAADCWDAEIESSYGWI 449
           AKGTVNNETLGYFIGRVYLFLTRLGIDK RLRFRQHLANEMAHYAADCWDAEIESSYGWI
Sbjct: 395 AKGTVNNETLGYFIGRVYLFLTRLGIDKERLRFRQHLANEMAHYAADCWDAEIESSYGWI 454

Query: 450 ECVGIADRSAYDLHAHTEKSGVPLVAHEKFAEPREVEKLVIAPVKKELGLAFKGSQKNVV 509
           ECVGIADRSAYDL AH++KSG PLVA EKFAEP+EVEKLVI PVKKELGLAFKG+QKNVV
Sbjct: 455 ECVGIADRSAYDLRAHSDKSGTPLVAEEKFAEPKEVEKLVITPVKKELGLAFKGNQKNVV 514

Query: 510 EALEAMKEKEALEMKANLESNGEVEFYVCTLGKNVLIKKSMVAISKEKKKEHQRVFTPSV 569
           E+LEAM E+EA+EMKA LES GEVEFYVCTL K+V IKK+MV+ISKEKKKEHQRVFTPSV
Sbjct: 515 ESLEAMNEEEAMEMKATLESKGEVEFYVCTLKKSVNIKKNMVSISKEKKKEHQRVFTPSV 574

Query: 570 IEPSFGIGRIIYCLFEHSYYMRPSKGGDEQLNVFRFPPLVAPIKCTVFPLVQNEQYEQVA 629
           IEPSFGIGRIIYCL+EH +  RPSK GDEQLN+FRFPPLVAPIKCTVFPLVQN+Q+E+VA
Sbjct: 575 IEPSFGIGRIIYCLYEHCFSTRPSKAGDEQLNLFRFPPLVAPIKCTVFPLVQNQQFEEVA 634

Query: 630 KVISKSLTLSGISHKIDITGTSIGKRYARTDELGVPFAITVDSTSSVTIRERDSKDQIRV 689
           KVISK L   GISHKIDITGTSIGKRYARTDELGVPFAITVDS +SVTIRERDSKDQ+RV
Sbjct: 635 KVISKELASVGISHKIDITGTSIGKRYARTDELGVPFAITVDSDTSVTIRERDSKDQVRV 694

Query: 690 SVEEAASVVKDVTDGLRTWENVWSTFPHHASAAAED 726
           +++EAASVV  V++G  TW++VW+TFPHH+SAAA++
Sbjct: 695 TLKEAASVVSSVSEGKMTWQDVWATFPHHSSAAADE 729

BLAST of CmaCh08G006050 vs. TAIR 10
Match: AT1G29870.1 (tRNA synthetase class II (G, H, P and S) family protein )

HSP 1 Score: 563.5 bits (1451), Expect = 2.4e-160
Identity = 307/470 (65.32%), Postives = 349/470 (74.26%), Query Frame = 0

Query: 46  LSQKQSAVDAQANAVRALKAAKAAKAEIDAAIEALNGLKIEKASIEKKLQAVVTGVGGDG 105
           L +K  AV+ Q  AV  L+ + AAK E +AAIE  N  K EK+++EK             
Sbjct: 9   LREKSLAVEDQELAVGTLEDSHAAKPETNAAIELPNKSKPEKSAVEK------------- 68

Query: 106 ALNRESFRQAVNNTLERRLFYIPSFKIYRGVAGLYDYGPPGCAVKSNVLAFWRQHFVLEE 165
             +RE FR+AV  TL+R LF   SF IY GVAGLYD+GP G  V+ N+L+ WR+ FV EE
Sbjct: 69  --DREDFREAVVKTLDRLLFVHKSFDIYHGVAGLYDFGPHGRTVELNILSLWRKCFVDEE 128

Query: 166 NMLEVDCPCVTPEAVLKASGHVDKFTDLMVKDEKTGTCYRADHLLKDFCNEKLQKDLSIS 225
           +M+EV C  +TPEAV  ASGHV KFTDLMVKDE  G  +RADHL+K +C E  +KD +IS
Sbjct: 129 DMMEVACTALTPEAVFNASGHVKKFTDLMVKDEVDGAFHRADHLVKSYC-ENRKKDPTIS 188

Query: 226 SEKAAEFKHVLAIMDDLSAEQLGAKIKEYGIVAPDTKNPLS-DPYPFNLMFQTSIGPSGL 285
           +E AAE   V+A ++DLSAE+LG  +  +   AP TKNPLS  P PFNLMFQTS G SG 
Sbjct: 189 AENAAELDKVIAHVEDLSAEELGG-VWNHCSTAPVTKNPLSHPPRPFNLMFQTSFGASGS 248

Query: 286 LPGYMRPETAQGIFVNFKDLYYYNGNKLPFAAAQIGQAFRNEISPRQGLLRVREFTLAEI 345
           L GY+RPETAQG F NFKD Y  NG KLPFA AQ+G+ FRNEISPRQGLLR REFTLAEI
Sbjct: 249 LIGYLRPETAQGSFCNFKDYYNLNGRKLPFAVAQVGRVFRNEISPRQGLLRTREFTLAEI 308

Query: 346 EHFVDPEDKSHPKFTDVANLEFLMFPREEQ-MSGKSARIIPLGEAVAKGTVNNETLGYFI 405
           EHFV PE KSH KF+DVA LE LMFPREEQ   G+ A+ + LGEAVAKG VN+ETLG+FI
Sbjct: 309 EHFVHPEHKSHSKFSDVAKLELLMFPREEQEKPGQFAKRLCLGEAVAKGHVNSETLGFFI 368

Query: 406 GRVYLFLTRLGIDKNRLRFRQHLANEMAHYAADCWDAEIESSYGWIECVGIADRSAYDLH 465
           GRVYLFL RLGIDK RLRFR HLANEMAHYA DCWDAEIE SYGWIECVGIADRS YDL 
Sbjct: 369 GRVYLFLIRLGIDKERLRFRHHLANEMAHYATDCWDAEIECSYGWIECVGIADRSDYDLR 428

Query: 466 AHTEKSGVPLVAHEKFAEPREVEKLVIAPVKKELGLAFKGSQKNVVEALE 514
           AH+EKSG  LVA EK AEP EVEKL I P  KELG AFKG+QKNVVEALE
Sbjct: 429 AHSEKSGHALVAQEKLAEPIEVEKLAITPEMKELGPAFKGNQKNVVEALE 461

BLAST of CmaCh08G006050 vs. TAIR 10
Match: AT3G44740.1 (Class II aaRS and biotin synthetases superfamily protein )

HSP 1 Score: 243.8 bits (621), Expect = 4.2e-64
Identity = 118/144 (81.94%), Postives = 127/144 (88.19%), Query Frame = 0

Query: 323 FRNEISPRQGLLRVREFTLAEIEHFVDPEDKSHPKFTDVANLEFLMFPREEQMSGKSARI 382
           FRNEIS RQGLLRV EFTLAEIEHFVDP +KSHPKF+DVAN EFLMFPREEQMSG+SA+ 
Sbjct: 87  FRNEISHRQGLLRVCEFTLAEIEHFVDPGNKSHPKFSDVANFEFLMFPREEQMSGQSAKK 146

Query: 383 IPLGEAVAKGTVNNETLGYFIGRVYLFLTRLGIDKNRLRFRQHLANEMAHYAADCWDAEI 442
           + LGE VAKGTVN ET+GYFI RVYLFL RLG DK +LRFRQH ANEMA YAADC DAE 
Sbjct: 147 LCLGEVVAKGTVNKETVGYFIARVYLFLVRLGTDKEQLRFRQHFANEMARYAADCLDAEF 206

Query: 443 ESSYGWIECVGIADRSAYDLHAHT 467
           ESSYGWIECVGIADRSA+DL AH+
Sbjct: 207 ESSYGWIECVGIADRSAFDLRAHS 230

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O236270.0e+0083.05Glycine--tRNA ligase, mitochondrial 1 OS=Arabidopsis thaliana OX=3702 GN=At1g298... [more]
Q5I0G46.5e-18750.29Glycine--tRNA ligase OS=Rattus norvegicus OX=10116 GN=Gars1 PE=1 SV=2[more]
Q9CZD31.9e-18650.15Glycine--tRNA ligase OS=Mus musculus OX=10090 GN=Gars1 PE=1 SV=1[more]
Q5RBL12.0e-18348.67Glycine--tRNA ligase OS=Pongo abelii OX=9601 GN=GARS1 PE=2 SV=1[more]
P412502.6e-18348.39Glycine--tRNA ligase OS=Homo sapiens OX=9606 GN=GARS1 PE=1 SV=3[more]
Match NameE-valueIdentityDescription
AT1G29880.10.0e+0083.05glycyl-tRNA synthetase / glycine--tRNA ligase [more]
AT1G29870.12.4e-16065.32tRNA synthetase class II (G, H, P and S) family protein [more]
AT3G44740.14.2e-6481.94Class II aaRS and biotin synthetases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 66..93
NoneNo IPR availableGENE3D3.30.40.230coord: 189..273
e-value: 6.8E-177
score: 590.0
NoneNo IPR availableGENE3D3.30.930.10Bira Bifunctional Protein; Domain 2coord: 116..479
e-value: 6.8E-177
score: 590.0
NoneNo IPR availableGENE3D1.10.287.10coord: 40..91
e-value: 1.2E-6
score: 30.3
NoneNo IPR availableGENE3D3.30.720.200coord: 490..558
e-value: 4.5E-17
score: 63.9
NoneNo IPR availablePANTHERPTHR10745:SF20GLYCINE--TRNA LIGASE 1, MITOCHONDRIALcoord: 28..725
NoneNo IPR availableCDDcd00858GlyRS_anticodoncoord: 583..703
e-value: 4.18064E-50
score: 168.89
NoneNo IPR availableSUPERFAMILY55681Class II aaRS and biotin synthetasescoord: 113..616
NoneNo IPR availableSUPERFAMILY52954Class II aaRS ABD-relatedcoord: 601..717
IPR002315Glycyl-tRNA synthetasePRINTSPR01043TRNASYNTHGLYcoord: 652..671
score: 71.67
coord: 132..146
score: 65.56
coord: 269..281
score: 65.81
coord: 288..305
score: 71.6
coord: 320..337
score: 69.14
coord: 567..579
score: 74.36
coord: 337..347
score: 69.7
IPR002315Glycyl-tRNA synthetaseTIGRFAMTIGR00389TIGR00389coord: 115..700
e-value: 9.5E-206
score: 682.6
IPR000738WHEP-TRS domainSMARTSM00991WHEP_TRS_a_2_a_3_acoord: 49..106
e-value: 3.5E-6
score: 36.5
IPR000738WHEP-TRS domainPROSITEPS51185WHEP_TRS_2coord: 45..101
score: 10.592379
IPR002314Aminoacyl-tRNA synthetase, class II (G/ P/ S/T)PFAMPF00587tRNA-synt_2bcoord: 288..585
e-value: 1.5E-9
score: 38.1
IPR036621Anticodon-binding domain superfamilyGENE3D3.40.50.800coord: 611..722
e-value: 3.1E-38
score: 132.2
IPR004154Anticodon-bindingPFAMPF03129HGTP_anticodoncoord: 613..699
e-value: 1.4E-18
score: 66.8
IPR027031Glycyl-tRNA synthetase/DNA polymerase subunit gamma-2PANTHERPTHR10745GLYCYL-TRNA SYNTHETASE/DNA POLYMERASE SUBUNIT GAMMA-2coord: 28..725
IPR006195Aminoacyl-tRNA synthetase, class IIPROSITEPS50862AA_TRNA_LIGASE_IIcoord: 251..592
score: 13.209898
IPR033731Glycyl-tRNA synthetase-like core domainCDDcd00774GlyRS-like_corecoord: 118..462
e-value: 1.22293E-110
score: 333.788
IPR009068S15/NS1, RNA-bindingSUPERFAMILY47060S15/NS1 RNA-binding domaincoord: 51..95

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh08G006050.1CmaCh08G006050.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0070150 mitochondrial glycyl-tRNA aminoacylation
biological_process GO:0006426 glycyl-tRNA aminoacylation
biological_process GO:0006418 tRNA aminoacylation for protein translation
cellular_component GO:0005739 mitochondrion
cellular_component GO:0005737 cytoplasm
molecular_function GO:0005524 ATP binding
molecular_function GO:0004820 glycine-tRNA ligase activity
molecular_function GO:0016740 transferase activity
molecular_function GO:0004812 aminoacyl-tRNA ligase activity
molecular_function GO:0000166 nucleotide binding