CmaCh08G005600 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh08G005600
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
DescriptionCitrate synthase
LocationCma_Chr08: 3194348 .. 3199896 (-)
RNA-Seq ExpressionCmaCh08G005600
SyntenyCmaCh08G005600
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGTTCTTCAAAAGTTTGACGGCTCTTTCAAAGCTACGGTCTCGTGTTGTGAGTATATTGTCTCGGGATTCTTCATGTTGTTGGTAATTTGTTAGTTATTTTGACTGTAAAGGTTTTAACGATCTGGAAATTTCCTCGATGCTTCAGGGACAGCAGTCTAATCTCAGTAATTCTGTTAGATGGCTTCAAATGCAGAGCTCACCGGATCTTGTAATATTCTCCACTCGCATTAATCTTTATTCTTCAATCTTGTTTGGGTAGACTACCGTTTTAAAGCTTTGAGACTTCATTTTGGGGTAGATCTAACATTTATGAATTGTTTAAATCGGACTGTGACGGCTACAATTCCCTGCTAGCCAGAGTTGATAACTGTTGACATTAAGTCTACGTGCATATTTCCAATTGGTTGATCTTTGGCAATTGAGTAGTCAGTTACCTAACAGATGGAATGATTACTTCTATCCAATAAGTGATTTCGCTTATAAGTTCGGTTTGTCGTGCAGGACCTACAATCTCATTTGAGGGAATTAATTCCAGAACAGCAGGTTTGGTTATCCTAACAGTATTCATTTAATAATTATTTAGCATAGCACTCCCACAATAAATACGAAGGCCTTCGTTCTTATTCTTTAAAGTTTTACACTGACGTCCAAGTTGATTCACTCATCTGCCTTTCATTGAGTATGACCACTTCACTGAAATGGGATGCATGTTTTTGACATAAATTCATCAGGAGATTACTTTAGATGCTTTTTTCCTGTATCCAGTTTGGTTGAAAGTTATTATAAGTCTACAAGAGTGTGACTAATTGATTCAATGCATTTACTGCCGACCTTGACCTAATTTTCCCCGCTAATTATATATTTCATTTATATATATTCATAATATGTTTAATGATTATAGGATCGTCTGAAGAAATTCAAAGCAGAACATGGAAAGGTTCAACTGGGCAACATCACTGTTGATATGGTAAGTATATTTGCTGAACTATTCTATATTTTGCTGCTCTCTAACATCGGTGTCATTTAGTCCCATGTGATAAATTCGTTCCAGGGAGCTCTTATGAAAAATAATCTCTTATCTTTTCTGCTAGCATTTTCTTTACATCTCTTAATAAATGCCTTGTCTCGAACATTGTGAAGGTACTTGGTGGAATGAGAGGAATGACAGGGCTACTATGGGAAACATCTCTACTTGACCCTGACGAGGTTTAGAATCCTTTTGGTTAATACCCAGTGAATTGTTTTGGTCTTTAAATGTCATTTGTTAAGGTTTCTGTGCTCGACATTCATGGTTATCTCTGGTCTATATGAACTGGATGAATGCTGGTTTTAGTATAGTAATGGATTTGGTGTATTATATTTCCAGGGTATTCGCTTTAGGGGTTTATCAATTCCTGAATGTCAAAAATTACTACCAGCTGCAAAGCCTGATGGAGAGCCACTACCTGAGGGTCTCCTGTGGCTTCTTCTGACAGGAAAGGTGAGTTGCCAAAAACTTGTCCATTCGTTTGATCTTTTGGTAATTATATCAGTGCTATAGAGGCATCAGGCATGTAGCTGATAGAATAAACCTACTTCTCCTTGTAGGCAGTATTTTGCTTTATACTTCCAAAAACATCATCATTTATTCTTTTATATATCAGGTACCAAGCAAAGAGCAAGTAGATGCACTATCCAAGGAGTTACAAAGCCGAGCTGTTGTTCCAGGTTCAGCTTGTTCATGATTGTTGCATGAGCATATTTGATTATATATGACTTCCGGAAGCCTCTTCTGCTCCAGCAGACATTTTCCTAAATGGTGGGTTTTTGTCATTTCTGGTTGCAGATTATGTATACAAGGCCATCGATGCTCTTCCCGTTACATCTCATCCGATGACTCAGTTTGCCACAGGTGTTATGGGTCTTCAGGTTAGTAATGAGTTCTCTATTTATCACGCCCTAGATGAGGTGATGATTTTCTTTGTCAACTTTACGTGAAAGGTTTTTGTATGGATACTTGGATCAATTTGGAAATTTCATATTTGACTTCCATAAATACTTTTTAGCATCCAACTTCTCGTTTGTTTATTTTGATCAAAACACAGCGTTTTATGCTTGGATCCAATTTTATAAACCCTGTACTGCTGAATTTAGTTTGTTGGATTATATTGAGAAAAAGTTTAGTTTATTGGAAACGTAATTAATTATCTAGGTGCAATGTTGTTAGCTGTCTTTTAAGGTGTCTACTTGTACTTTCCTTCAGGTTCAAAGTGAATTCCAGAAGGCTTATGAGAAAGGAATTCATAAATCAAAGTAAAGTTCGTGCATTCAAGATTCAGTCTTCGTATTTTCTCAAGTACTGAAAGCTATTTAATTTTATAGGTACTGGGAACCAACGTATGAAGATTCTATGAATTTGATTGCTCGAGTACCTTTGGTAGCTTCTTATGTCTACCGCAGGTTGGTAGATCACTCTAATTATATATTTGTTGATTGTATGAAACTAATTGTGTCACTTGCCGTTGCATATTTAAAATATTCTGACCCCTTCTTGCTGTCTCCTCCTTGTTTCGTTGTTGGATTAATTTGGACTTCCTTCTAATCATATTTGAAGGATATACAAGGATGGGCATATAATTCCAAAAGATGACTCTCTGGATTATGGTGGCAATTTTTCGCACATGTTAGGATTTGATAGTCCCCAAATGCAAGAACTCATGAGGCTCTATGTTACTATCCATACGTAAGTATATGTTGTTCATTTCCACCGGATTTAAAGTATTTTAATTTAAGAGAGAATAATCAAATAACATCTAATGTTTGTGTAAAAAATGACCTGCAGTGATCATGAAGGAGGGAATGTCAGCGCTCACACAGGCCACCTCGTAAGTTATCTTTCTTCTCAGATTTCAATTGAATTGTGTGTTTTCTTTTCTCTTTAGTATTTTATAGTATCGTTTCCTTAGTCTGATCTTCTGCTGTACATTGATTGAAACATGACTTTTTCAAGCGTAGCTGTCAATGTAATGTTTCTGCACATCAAATTCATGACTGTGTCATTCTCTTAGTTATTTAATACTTTTATGCAGGTTGCTAGTGCACTTTCAGATCCCTATCTTTCGTTTGCTGCTGCTCTAAACGGATTGGCTGGACCATTACATGGTTTGGCAAATCAGGTGTGTGATAGACTGTTTTCTAATAGCTACTCCTTTTCCTCTATTTGGTTTGAACTTTTAATAAGTACGTAATTATCATTTGTAACTCTCTCATTTTATTGATGGATTTCATTCTTACTACTATTTTCCCCCTCAATTCATTGACTTTCAATTTATCTAAAAAGTGTAAAATAGAAATTAATAGATAAGTGAAAGGGATTGAGATAATTATTTGGTTTACTCATCATAACTGATAACAGTCTAATAGTACATAGCACTTGTTTTGTTCATTTTAGGACTGTAGATTTGAAACCAAGTAATGTTTGAGCGTTAGCCTTAAACAAACTCCCATTAACTTTATGACATGAATATACATAGCAAATGTTTGCTCTATGCTTCTTATCAACATAAAAAGACTGCCAATTATTGTTGCTTATTTCCCACTGCTTGCAGGAAGTTTTACTTTGGATCAAATCTGTAGTGGACGAGTGTGGAGAGAACATAACTAAGGAGCAGTTGAAAGACTATGTTTGGAAAACTCTAAACAGTGGCAAGGTAACAACATAAATCCATTCTGGCTAGAAATGTCAGGCATGGAGCTTATACTTAATATACCTTCATCCTCGCTTATATATAGCTAGTATCAGTAACTGTACTGTTTTAGCCTTCAAGCATTTCAGTCATAGTGGTTTATTTTTTCTGGGAGATATTTTTTCCCCGTTTTCATGAACTCCAATGTATTTCGGGTCTTATCACTATAGGAAGAGGAGACGTCCTTTACGGATTTATGGTTACATATTCATTTGTGGAATTTTCATCAACTCCTTATCATTCTTTTTAATCGTAGGTTGTTCCAGGATTTGGTCACGGAGTTCTGCGTAAAACAGATCCAAGATATACTTGTCAGAGAGAGTTTGCATTGAAGCATTTGCCAGACGATCCTCTTTTTCAGCTGGTTGTCATTTTCTCCCTGATTGTAGACTCACACACAAGCATTCATACACACGCACTGTATAAATCCCCTTTGGTTTACTTAAGCCGTAAATTGCTCACACAATAACCTAGCATGGGATTTTGCAGGTATCAAAGCTCTACGAAGTCGTGCCACCCATCCTAACAGAACTTGGCAAGGCAAGATGAGCTTCCTTCTCTATAACTAGCTCAATTGTGCATGCTCCTTATCTTCTGTTGTTAGTCATTTCTGCTAGCATTTGTGGGTTCATTTCTCAACTCGGGTGGGGGAGTTTCAGTAGTATTAAATTCTTCTTGTGATAATTTCGGCTGTCTACCAAACATATAGATTCCATGAAACTGATTCTCTCCCTTCATAAAATGAATTAAATGGTCTATGTTTGTACTAAATGTCCAAGATCGAGCACAATATGTAACTTGACTTTTGAGTCGAACACAGTATATTTGATCAAGTACATGAAACTATACCATGAATTGTTGATGCTCACGAACGTCACCCATGAATCTTTTACATAGTTGTCTCCTCTTGTGGCATTTTCCTCGTTTGCAGGTTAAAAACCCATGGCCTAATGTTGATGCTCATAGTGGTGTGCTGTTGAACTACTTTGGTTTGACCGAAGCAAGGTAGAATGCGATTCAATTAATACACATTGATGTTTACCTTTAACTAAACGATAACTATAACTTTCATCGACTATGCACAGGTACTTTACTGTTCTCTTCGGTGTTTCAAGGAGTATTGGGATATGTTCTCAGGTTTGTCTTTTTTGTTTTTTGTACATTCATTCTCAATTCTTCTTAATTTTACCAAATTCTTAAAGATTTACTACATGTTCTGGCTTCAGTTAATATGGGACCGAGCTCTGGGGTTGCCACTCGAGAGGCCGAAGAGCGTGACATTGAAATGGCTCGAGGACCACTGCAAGAAAGCAGCAGCTTGATTGAAAGTGGGTGTCCAAAAATAGTTCATTCAGCCTTCACAAATTGGTCGAGGGTGAGAGATTTCTTGTCATTTGCTAGTTTTAATAATTGTTCAAGCTTGGTTTACCGCGCAGATACAGGATTACCTGAAGTTACTTTTGGGGATAAGTAGATTTTGGAATCACTTATTTCTTAAACTCGCCCGAGTTTCTTGGCAAATTTTTCATTCATTCGGTTTCATATAGTAGCTGTTAGAAGTGCATTGTTCAGGAGAGGAATTGGTCGGGATGCCATTTGAATAAAACGGTTGGCATGGCTTGAATGTTAATCTATACCCCCATTAGGAGCTGAGATTCGGCTGCACTTTACCTTGTATGAGACATTTCAACGTAAATTTAGAATGATTTCGAAGTGTTAAAACTTTTTTTAATTACTTTTTAAAAGCCCCTTCATACTGTCCTCAAGCCTATGTACGTAACTTAGCAATAAAGTTTAAAACATATTTTTTATATGGGAA

mRNA sequence

ATGGCGTTCTTCAAAAGTTTGACGGCTCTTTCAAAGCTACGGTCTCGTGTTGGACAGCAGTCTAATCTCAGTAATTCTGTTAGATGGCTTCAAATGCAGAGCTCACCGGATCTTGACCTACAATCTCATTTGAGGGAATTAATTCCAGAACAGCAGGATCGTCTGAAGAAATTCAAAGCAGAACATGGAAAGGTTCAACTGGGCAACATCACTGTTGATATGGTACTTGGTGGAATGAGAGGAATGACAGGGCTACTATGGGAAACATCTCTACTTGACCCTGACGAGGGTATTCGCTTTAGGGGTTTATCAATTCCTGAATGTCAAAAATTACTACCAGCTGCAAAGCCTGATGGAGAGCCACTACCTGAGGGTCTCCTGTGGCTTCTTCTGACAGGAAAGGTACCAAGCAAAGAGCAAGTAGATGCACTATCCAAGGAGTTACAAAGCCGAGCTGTTGTTCCAGATTATGTATACAAGGCCATCGATGCTCTTCCCGTTACATCTCATCCGATGACTCAGTTTGCCACAGGTGTTATGGGTCTTCAGGTTCAAAGTGAATTCCAGAAGGCTTATGAGAAAGGAATTCATAAATCAAAGTACTGGGAACCAACGTATGAAGATTCTATGAATTTGATTGCTCGAGTACCTTTGGTAGCTTCTTATGTCTACCGCAGGATATACAAGGATGGGCATATAATTCCAAAAGATGACTCTCTGGATTATGGTGGCAATTTTTCGCACATGTTAGGATTTGATAGTCCCCAAATGCAAGAACTCATGAGGCTCTATGTTACTATCCATACTGATCATGAAGGAGGGAATGTCAGCGCTCACACAGGCCACCTCGTTGCTAGTGCACTTTCAGATCCCTATCTTTCGTTTGCTGCTGCTCTAAACGGATTGGCTGGACCATTACATGGTTTGGCAAATCAGGAAGTTTTACTTTGGATCAAATCTGTAGTGGACGAGTGTGGAGAGAACATAACTAAGGAGCAGTTGAAAGACTATGTTTGGAAAACTCTAAACAGTGGCAAGGTTGTTCCAGGATTTGGTCACGGAGTTCTGCGTAAAACAGATCCAAGATATACTTGTCAGAGAGAGTTTGCATTGAAGCATTTGCCAGACGATCCTCTTTTTCAGCTGGTTAAAAACCCATGGCCTAATGTTGATGCTCATAGTGGTGTGCTGTTGAACTACTTTGGTTTGACCGAAGCAAGGTACTTTACTGTTCTCTTCGGTGTTTCAAGGAGTATTGGGATATGTTCTCAGTTAATATGGGACCGAGCTCTGGGGTTGCCACTCGAGAGGCCGAAGAGCGTGACATTGAAATGGCTCGAGGACCACTGCAAGAAAGCAGCAGCTTGATTGAAAATACAGGATTACCTGAAGTTACTTTTGGGGATAAGTAGATTTTGGAATCACTTATTTCTTAAACTCGCCCGAGTTTCTTGGCAAATTTTTCATTCATTCGGTTTCATATAGTAGCTGTTAGAAGTGCATTGTTCAGGAGAGGAATTGGTCGGGATGCCATTTGAATAAAACGGTTGGCATGGCTTGAATGTTAATCTATACCCCCATTAGGAGCTGAGATTCGGCTGCACTTTACCTTGTATGAGACATTTCAACGTAAATTTAGAATGATTTCGAAGTGTTAAAACTTTTTTTAATTACTTTTTAAAAGCCCCTTCATACTGTCCTCAAGCCTATGTACGTAACTTAGCAATAAAGTTTAAAACATATTTTTTATATGGGAA

Coding sequence (CDS)

ATGGCGTTCTTCAAAAGTTTGACGGCTCTTTCAAAGCTACGGTCTCGTGTTGGACAGCAGTCTAATCTCAGTAATTCTGTTAGATGGCTTCAAATGCAGAGCTCACCGGATCTTGACCTACAATCTCATTTGAGGGAATTAATTCCAGAACAGCAGGATCGTCTGAAGAAATTCAAAGCAGAACATGGAAAGGTTCAACTGGGCAACATCACTGTTGATATGGTACTTGGTGGAATGAGAGGAATGACAGGGCTACTATGGGAAACATCTCTACTTGACCCTGACGAGGGTATTCGCTTTAGGGGTTTATCAATTCCTGAATGTCAAAAATTACTACCAGCTGCAAAGCCTGATGGAGAGCCACTACCTGAGGGTCTCCTGTGGCTTCTTCTGACAGGAAAGGTACCAAGCAAAGAGCAAGTAGATGCACTATCCAAGGAGTTACAAAGCCGAGCTGTTGTTCCAGATTATGTATACAAGGCCATCGATGCTCTTCCCGTTACATCTCATCCGATGACTCAGTTTGCCACAGGTGTTATGGGTCTTCAGGTTCAAAGTGAATTCCAGAAGGCTTATGAGAAAGGAATTCATAAATCAAAGTACTGGGAACCAACGTATGAAGATTCTATGAATTTGATTGCTCGAGTACCTTTGGTAGCTTCTTATGTCTACCGCAGGATATACAAGGATGGGCATATAATTCCAAAAGATGACTCTCTGGATTATGGTGGCAATTTTTCGCACATGTTAGGATTTGATAGTCCCCAAATGCAAGAACTCATGAGGCTCTATGTTACTATCCATACTGATCATGAAGGAGGGAATGTCAGCGCTCACACAGGCCACCTCGTTGCTAGTGCACTTTCAGATCCCTATCTTTCGTTTGCTGCTGCTCTAAACGGATTGGCTGGACCATTACATGGTTTGGCAAATCAGGAAGTTTTACTTTGGATCAAATCTGTAGTGGACGAGTGTGGAGAGAACATAACTAAGGAGCAGTTGAAAGACTATGTTTGGAAAACTCTAAACAGTGGCAAGGTTGTTCCAGGATTTGGTCACGGAGTTCTGCGTAAAACAGATCCAAGATATACTTGTCAGAGAGAGTTTGCATTGAAGCATTTGCCAGACGATCCTCTTTTTCAGCTGGTTAAAAACCCATGGCCTAATGTTGATGCTCATAGTGGTGTGCTGTTGAACTACTTTGGTTTGACCGAAGCAAGGTACTTTACTGTTCTCTTCGGTGTTTCAAGGAGTATTGGGATATGTTCTCAGTTAATATGGGACCGAGCTCTGGGGTTGCCACTCGAGAGGCCGAAGAGCGTGACATTGAAATGGCTCGAGGACCACTGCAAGAAAGCAGCAGCTTGA

Protein sequence

MAFFKSLTALSKLRSRVGQQSNLSNSVRWLQMQSSPDLDLQSHLRELIPEQQDRLKKFKAEHGKVQLGNITVDMVLGGMRGMTGLLWETSLLDPDEGIRFRGLSIPECQKLLPAAKPDGEPLPEGLLWLLLTGKVPSKEQVDALSKELQSRAVVPDYVYKAIDALPVTSHPMTQFATGVMGLQVQSEFQKAYEKGIHKSKYWEPTYEDSMNLIARVPLVASYVYRRIYKDGHIIPKDDSLDYGGNFSHMLGFDSPQMQELMRLYVTIHTDHEGGNVSAHTGHLVASALSDPYLSFAAALNGLAGPLHGLANQEVLLWIKSVVDECGENITKEQLKDYVWKTLNSGKVVPGFGHGVLRKTDPRYTCQREFALKHLPDDPLFQLVKNPWPNVDAHSGVLLNYFGLTEARYFTVLFGVSRSIGICSQLIWDRALGLPLERPKSVTLKWLEDHCKKAAA
Homology
BLAST of CmaCh08G005600 vs. ExPASy Swiss-Prot
Match: P49298 (Citrate synthase, mitochondrial OS=Citrus maxima OX=37334 GN=CIT PE=2 SV=1)

HSP 1 Score: 828.6 bits (2139), Expect = 3.5e-239
Identity = 403/471 (85.56%), Postives = 430/471 (91.30%), Query Frame = 0

Query: 1   MAFFKSLTALSKLRSRVGQQSNLSNSVRWLQMQSSPDLDLQSHLRELIPEQQDRLKKFKA 60
           MA  +S TALS+LRSR GQQSNLSNSVRWLQMQSS DLDL S L+E+IPEQQ+RLKK K+
Sbjct: 1   MASLRSATALSRLRSRAGQQSNLSNSVRWLQMQSSADLDLHSQLKEMIPEQQERLKKVKS 60

Query: 61  EHGKVQLGNITVDMVLGGMRGMTGLLWETSLLDPDEGIRFRGLSIPECQKLLPAAKPDGE 120
           + GK QLGNIT+D+V+GGMRGMTGLLWETSLLDPDEGIRFRGLSIPECQKLLPAAKPDGE
Sbjct: 61  DLGKAQLGNITIDVVIGGMRGMTGLLWETSLLDPDEGIRFRGLSIPECQKLLPAAKPDGE 120

Query: 121 PLPEGLLWLLLTGKVPSKEQVDALSKELQSRAVVPDYVYKAIDALPVTSHPMTQFATGVM 180
           PLPEGLLWLLLTGKVPSKEQVD LSKEL+ RA VPDYVYKAIDALPV++HPMTQFA+GVM
Sbjct: 121 PLPEGLLWLLLTGKVPSKEQVDGLSKELRDRATVPDYVYKAIDALPVSAHPMTQFASGVM 180

Query: 181 GLQVQSEFQKAYEKGIHKSKYWEPTYEDSMNLIARVPLVASYVYRRIYKDGHIIPKDDSL 240
            LQVQSEFQ+AYEKGIHKSK WEPT EDS+NLIARVP+VA+YVY+RIYKDG IIPKDDSL
Sbjct: 181 ALQVQSEFQEAYEKGIHKSKSWEPTSEDSLNLIARVPVVAAYVYQRIYKDGKIIPKDDSL 240

Query: 241 DYGGNFSHMLGFDSPQMQELMRLYVTIHTDHEGGNVSAHTGHLVASALSDPYLSFAAALN 300
           DYGGNFSHMLGFD P+M ELMRLYVTIH+DHEGGNVSAHTGHLVASALSDPYLSF AALN
Sbjct: 241 DYGGNFSHMLGFDDPKMLELMRLYVTIHSDHEGGNVSAHTGHLVASALSDPYLSFLAALN 300

Query: 301 GLAGPLHGLANQEVLLWIKSVVDECGENITKEQLKDYVWKTLNSGKVVPGFGHGVLRKTD 360
           GLAGPLHGLANQEVLLWIKSVVDECGEN+T EQLKDYVWKTLNSGKVVPGFGHGVLRKTD
Sbjct: 301 GLAGPLHGLANQEVLLWIKSVVDECGENVTTEQLKDYVWKTLNSGKVVPGFGHGVLRKTD 360

Query: 361 PRYTCQREFALKHLPDDPLFQL-----------------VKNPWPNVDAHSGVLLNYFGL 420
           PRYTCQREFALKHLPDDPLFQL                 VKNPWPNVDAHSGVLLN+FGL
Sbjct: 361 PRYTCQREFALKHLPDDPLFQLVSKLYEVVPPILTKLGKVKNPWPNVDAHSGVLLNHFGL 420

Query: 421 TEARYFTVLFGVSRSIGICSQLIWDRALGLPLERPKSVTLKWLEDHCKKAA 455
            EARY+TVLFGVSRS+GICSQLIWDRALGLPLERPKSVTL W+E +CKKAA
Sbjct: 421 AEARYYTVLFGVSRSLGICSQLIWDRALGLPLERPKSVTLDWIEKNCKKAA 471

BLAST of CmaCh08G005600 vs. ExPASy Swiss-Prot
Match: O80433 (Citrate synthase, mitochondrial OS=Daucus carota OX=4039 GN=CS PE=2 SV=1)

HSP 1 Score: 811.2 bits (2094), Expect = 5.8e-234
Identity = 389/472 (82.42%), Postives = 430/472 (91.10%), Query Frame = 0

Query: 1   MAFFKSLTALSKLRSRVGQQSNLSNSVRWLQMQ-SSPDLDLQSHLRELIPEQQDRLKKFK 60
           M FF+S++ L+KLRSR  QQSNLSN+VRW Q+Q S+ DLDL+S L+ELIPEQQ+R+KK K
Sbjct: 1   MVFFRSVSLLNKLRSRAVQQSNLSNTVRWFQVQTSASDLDLRSQLKELIPEQQERIKKLK 60

Query: 61  AEHGKVQLGNITVDMVLGGMRGMTGLLWETSLLDPDEGIRFRGLSIPECQKLLPAAKPDG 120
           AEHGKVQLGNITVDMVLGGMRGMTGLLWETSLLDP+EGIRFRGLSIPECQKLLP AKP G
Sbjct: 61  AEHGKVQLGNITVDMVLGGMRGMTGLLWETSLLDPEEGIRFRGLSIPECQKLLPGAKPGG 120

Query: 121 EPLPEGLLWLLLTGKVPSKEQVDALSKELQSRAVVPDYVYKAIDALPVTSHPMTQFATGV 180
           EPLPEGLLWLLLTGKVP+KEQVDALS EL+SRA VP++VYK IDALPVT+HPMTQFATGV
Sbjct: 121 EPLPEGLLWLLLTGKVPTKEQVDALSAELRSRAAVPEHVYKTIDALPVTAHPMTQFATGV 180

Query: 181 MGLQVQSEFQKAYEKGIHKSKYWEPTYEDSMNLIARVPLVASYVYRRIYKDGHIIPKDDS 240
           M LQVQSEFQKAYEKGIHK+KYWEPTYEDS+ LIA++P+VA+Y+YRR+YK+G  I  DDS
Sbjct: 181 MALQVQSEFQKAYEKGIHKTKYWEPTYEDSITLIAQLPVVAAYIYRRMYKNGQSISTDDS 240

Query: 241 LDYGGNFSHMLGFDSPQMQELMRLYVTIHTDHEGGNVSAHTGHLVASALSDPYLSFAAAL 300
           LDYG NF+HMLG+DSP MQELMRLYVTIHTDHEGGNVSAHTGHLVASALSDPYLSFAAAL
Sbjct: 241 LDYGANFAHMLGYDSPSMQELMRLYVTIHTDHEGGNVSAHTGHLVASALSDPYLSFAAAL 300

Query: 301 NGLAGPLHGLANQEVLLWIKSVVDECGENITKEQLKDYVWKTLNSGKVVPGFGHGVLRKT 360
           NGLAGPLHGLANQEVLLWIKSVV ECGEN+TKEQLKDY+WKTLNSGKVVPG+GHGVLR T
Sbjct: 301 NGLAGPLHGLANQEVLLWIKSVVSECGENVTKEQLKDYIWKTLNSGKVVPGYGHGVLRNT 360

Query: 361 DPRYTCQREFALKHLPDDPLFQL-----------------VKNPWPNVDAHSGVLLNYFG 420
           DPRY CQREFALKHLPDDPLFQL                 VKNPWPNVDAHSGVLLN++G
Sbjct: 361 DPRYICQREFALKHLPDDPLFQLVSNLFEVVPPILTELGKVKNPWPNVDAHSGVLLNHYG 420

Query: 421 LTEARYFTVLFGVSRSIGICSQLIWDRALGLPLERPKSVTLKWLEDHCKKAA 455
           LTEARY+TVLFGVSR+IGICSQL+WDRALGLPLERPKSVT++WLE+HCKK++
Sbjct: 421 LTEARYYTVLFGVSRAIGICSQLVWDRALGLPLERPKSVTMEWLENHCKKSS 472

BLAST of CmaCh08G005600 vs. ExPASy Swiss-Prot
Match: P20115 (Citrate synthase 4, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=CSY4 PE=1 SV=3)

HSP 1 Score: 795.4 bits (2053), Expect = 3.3e-229
Identity = 383/473 (80.97%), Postives = 426/473 (90.06%), Query Frame = 0

Query: 1   MAFFKSLTALSKLRSRV-GQQSNLSNSVRWLQMQSSPDLDLQSHLRELIPEQQDRLKKFK 60
           M FF+S++A ++LRSRV GQQS+LSNSVRW+QMQSS DLDL+S L+ELIPEQQDRLKK K
Sbjct: 1   MVFFRSVSAFTRLRSRVQGQQSSLSNSVRWIQMQSSTDLDLKSQLQELIPEQQDRLKKLK 60

Query: 61  AEHGKVQLGNITVDMVLGGMRGMTGLLWETSLLDPDEGIRFRGLSIPECQKLLPAAKPDG 120
           +EHGKVQLGNITVDMV+GGMRGMTGLLWETSLLDP+EGIRFRGLSIPECQK+LP A+   
Sbjct: 61  SEHGKVQLGNITVDMVIGGMRGMTGLLWETSLLDPEEGIRFRGLSIPECQKVLPTAQSGA 120

Query: 121 EPLPEGLLWLLLTGKVPSKEQVDALSKELQSRAVVPDYVYKAIDALPVTSHPMTQFATGV 180
           EPLPEGLLWLLLTGKVPSKEQV+ALSK+L +RA VPDYVY AIDALP T+HPMTQFA+GV
Sbjct: 121 EPLPEGLLWLLLTGKVPSKEQVEALSKDLANRAAVPDYVYNAIDALPSTAHPMTQFASGV 180

Query: 181 MGLQVQSEFQKAYEKGIHKSKYWEPTYEDSMNLIARVPLVASYVYRRIYKDGHIIPKDDS 240
           M LQVQSEFQKAYE GIHKSK+WEPTYED +NLIARVP+VA+YVYRR+YK+G  IP D S
Sbjct: 181 MALQVQSEFQKAYENGIHKSKFWEPTYEDCLNLIARVPVVAAYVYRRMYKNGDSIPSDKS 240

Query: 241 LDYGGNFSHMLGFDSPQMQELMRLYVTIHTDHEGGNVSAHTGHLVASALSDPYLSFAAAL 300
           LDYG NFSHMLGFD  +++ELMRLY+TIH+DHEGGNVSAHTGHLV SALSDPYLSFAAAL
Sbjct: 241 LDYGANFSHMLGFDDEKVKELMRLYITIHSDHEGGNVSAHTGHLVGSALSDPYLSFAAAL 300

Query: 301 NGLAGPLHGLANQEVLLWIKSVVDECGENITKEQLKDYVWKTLNSGKVVPGFGHGVLRKT 360
           NGLAGPLHGLANQEVLLWIKSVV+ECGE+I+KEQLK+YVWKTLNSGKV+PG+GHGVLR T
Sbjct: 301 NGLAGPLHGLANQEVLLWIKSVVEECGEDISKEQLKEYVWKTLNSGKVIPGYGHGVLRNT 360

Query: 361 DPRYTCQREFALKHLPDDPLFQL-----------------VKNPWPNVDAHSGVLLNYFG 420
           DPRY CQREFALKHLPDDPLFQL                 VKNPWPNVDAHSGVLLN++G
Sbjct: 361 DPRYVCQREFALKHLPDDPLFQLVSKLYEVVPPVLTELGKVKNPWPNVDAHSGVLLNHYG 420

Query: 421 LTEARYFTVLFGVSRSIGICSQLIWDRALGLPLERPKSVTLKWLEDHCKKAAA 456
           LTEARY+TVLFGVSRS+GICSQLIWDRALGL LERPKSVT+ WLE HCKKA++
Sbjct: 421 LTEARYYTVLFGVSRSLGICSQLIWDRALGLALERPKSVTMDWLEAHCKKASS 473

BLAST of CmaCh08G005600 vs. ExPASy Swiss-Prot
Match: Q43175 (Citrate synthase, mitochondrial OS=Solanum tuberosum OX=4113 PE=2 SV=1)

HSP 1 Score: 755.4 bits (1949), Expect = 3.8e-217
Identity = 373/472 (79.03%), Postives = 414/472 (87.71%), Query Frame = 0

Query: 1   MAFFKSLTALSKLRSRVGQQSNLSNSVRWLQMQSSPDLDLQSHL-RELIPEQQDRLKKFK 60
           M F++S++ LSKLRSR  QQSN+SNSVRWLQ+Q+S  LDL+S L +ELIPEQQDRLKK K
Sbjct: 1   MVFYRSVSLLSKLRSRAVQQSNVSNSVRWLQVQTSSGLDLRSELVQELIPEQQDRLKKIK 60

Query: 61  AEHGKVQLGNITVDMVLGGMRGMTGLLWETSLLDPDEGIRFRGLSIPECQKLLPAAKPDG 120
           ++  K  +GNITVDMVLGGMRGMTGLLW+   LDPDEGIRFRGLSIPECQK+LPAAKP G
Sbjct: 61  SDM-KGSIGNITVDMVLGGMRGMTGLLWKPHYLDPDEGIRFRGLSIPECQKVLPAAKPGG 120

Query: 121 EPLPEGLLWLLLTGKVPSKEQVDALSKELQSRAVVPDYV-YKAIDALPVTSHPMTQFATG 180
           EPLPEGLLWLLLTGKVPSKEQV+++   +    ++   + Y  IDALPVT+HPMTQFATG
Sbjct: 121 EPLPEGLLWLLLTGKVPSKEQVNSIVSGIAESGIISLIIMYTTIDALPVTAHPMTQFATG 180

Query: 181 VMGLQVQSEFQKAYEKGIHKSKYWEPTYEDSMNLIARVPLVASYVYRRIYKDGHIIPKDD 240
           VM LQVQSEFQKAYEKGIHKSKYWEPTYEDSMNLIA+VPLVA+YVYRR+YK+G  IPKD+
Sbjct: 181 VMALQVQSEFQKAYEKGIHKSKYWEPTYEDSMNLIAQVPLVAAYVYRRMYKNGDTIPKDE 240

Query: 241 SLDYGGNFSHMLGFDSPQMQE-LMRLYVTIHTDHEGGNVSAHTGHLVASALSDPYLSFAA 300
           SLDYG NF+HMLGF S +M E LMRLYVTIH+DHEGGNVSAHTGHLVASALSDPYLSFAA
Sbjct: 241 SLDYGANFAHMLGFSSSEMHELLMRLYVTIHSDHEGGNVSAHTGHLVASALSDPYLSFAA 300

Query: 301 ALNGLAGPLHGLANQEVLLWIKSVVDECGENITKEQLKDYVWKTLNSGKVVPGFGHGVLR 360
           ALNGLAGPLHGLANQEVLLWIKSVV+ECGENI+KEQLKDYVWKTLNSGKVVPGFGHGVLR
Sbjct: 301 ALNGLAGPLHGLANQEVLLWIKSVVEECGENISKEQLKDYVWKTLNSGKVVPGFGHGVLR 360

Query: 361 KTDPRYTCQREFALKHLPDDPLFQLVK----------------NPWPNVDAHSGVLLNYF 420
           KT PRYTCQREFA+KHLP+DPLFQLV                  PWPNVDAHSGVLLNY+
Sbjct: 361 KTVPRYTCQREFAMKHLPEDPLFQLVSKLYEVFLLFLQNLAKLKPWPNVDAHSGVLLNYY 420

Query: 421 GLTEARYFTVLFGVSRSIGICSQLIWDRALGLPLERPKSVTLKWLEDHCKKA 454
           GLTEARY+TVLFGVSR++GICSQLIWDRALGLPLERPKSVT++WLE+ CKKA
Sbjct: 421 GLTEARYYTVLFGVSRALGICSQLIWDRALGLPLERPKSVTMEWLENQCKKA 471

BLAST of CmaCh08G005600 vs. ExPASy Swiss-Prot
Match: Q9M1D3 (Citrate synthase 5, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=CSY5 PE=1 SV=2)

HSP 1 Score: 749.6 bits (1934), Expect = 2.1e-215
Identity = 370/466 (79.40%), Postives = 409/466 (87.77%), Query Frame = 0

Query: 1   MAFFKSLTALSKLRSRVGQQSNLSNSVRWLQMQSSPDLDLQSHLRELIPEQQDRLKKFKA 60
           M FF+S++A+S+LRSR  QQS+LSNSVRWL    S +LDL+S ++E+IPEQQDRLKK K+
Sbjct: 1   MVFFRSVSAISRLRSRAVQQSSLSNSVRWLH---SSELDLKSQMQEIIPEQQDRLKKLKS 60

Query: 61  EHGKVQLGNITVDMVLGGMRGMTGLLWETSLLDPDEGIRFRGLSIPECQKLLPAAKPDGE 120
           E GKV +GNITVDMVLGGMRGMTGLLWETSLLD DEGIRFRG+SIPECQK+LP+A+   E
Sbjct: 61  EQGKVPVGNITVDMVLGGMRGMTGLLWETSLLDADEGIRFRGMSIPECQKILPSAESGEE 120

Query: 121 PLPEGLLWLLLTGKVPSKEQVDALSKELQSRAVVPDYVYKAIDALPVTSHPMTQFATGVM 180
           PLPE LLWLLLTGKVP+KEQ +ALS EL  RA VP     AIDALP T+HPMTQFA+GVM
Sbjct: 121 PLPESLLWLLLTGKVPTKEQANALSTELAHRAAVP-----AIDALPSTAHPMTQFASGVM 180

Query: 181 GLQVQSEFQKAYEKG-IHKSKYWEPTYEDSMNLIARVPLVASYVYRRIYKDGHIIPKDDS 240
            LQVQSEFQKAYE+G I KSKYWEPT+ED++NLIARVP+VASYVYRR+YKDG IIP DDS
Sbjct: 181 ALQVQSEFQKAYEQGDISKSKYWEPTFEDALNLIARVPVVASYVYRRMYKDGSIIPLDDS 240

Query: 241 LDYGGNFSHMLGFDSPQMQELMRLYVTIHTDHEGGNVSAHTGHLVASALSDPYLSFAAAL 300
           LDYG NFSHMLGFDSPQM+ELMRLYVTIH+DHEGGNVSAH GHLV SALSDPYLSFAAAL
Sbjct: 241 LDYGANFSHMLGFDSPQMKELMRLYVTIHSDHEGGNVSAHAGHLVGSALSDPYLSFAAAL 300

Query: 301 NGLAGPLHGLANQEVLLWIKSVVDECGENITKEQLKDYVWKTLNSGKVVPGFGHGVLRKT 360
           NGLAGPLHGLANQEVLLWIK VV+ECGE+I+KEQLKDYVWKTLNSGKVVPG+GHGVLRKT
Sbjct: 301 NGLAGPLHGLANQEVLLWIKLVVEECGESISKEQLKDYVWKTLNSGKVVPGYGHGVLRKT 360

Query: 361 DPRYTCQREFALKHLPDDPLFQL-----------------VKNPWPNVDAHSGVLLNYFG 420
           DPRY CQREFALKHLPDDPLFQL                 VKNPWPNVDAHSGVLLNY+G
Sbjct: 361 DPRYICQREFALKHLPDDPLFQLVSKLYEVVPPILTELGKVKNPWPNVDAHSGVLLNYYG 420

Query: 421 LTEARYFTVLFGVSRSIGICSQLIWDRALGLPLERPKSVTLKWLED 449
           LTEARY+TVLFGVSRS+GICSQLIWDRALGLPLERPKSV + WL++
Sbjct: 421 LTEARYYTVLFGVSRSLGICSQLIWDRALGLPLERPKSVNMDWLDN 458

BLAST of CmaCh08G005600 vs. TAIR 10
Match: AT2G44350.1 (Citrate synthase family protein )

HSP 1 Score: 800.0 bits (2065), Expect = 9.6e-232
Identity = 383/472 (81.14%), Postives = 426/472 (90.25%), Query Frame = 0

Query: 1   MAFFKSLTALSKLRSRVGQQSNLSNSVRWLQMQSSPDLDLQSHLRELIPEQQDRLKKFKA 60
           M FF+S++A ++LRSRVGQQS+LSNSVRW+QMQSS DLDL+S L+ELIPEQQDRLKK K+
Sbjct: 1   MVFFRSVSAFTRLRSRVGQQSSLSNSVRWIQMQSSTDLDLKSQLQELIPEQQDRLKKLKS 60

Query: 61  EHGKVQLGNITVDMVLGGMRGMTGLLWETSLLDPDEGIRFRGLSIPECQKLLPAAKPDGE 120
           EHGKVQLGNITVDMV+GGMRGMTGLLWETSLLDP+EGIRFRGLSIPECQK+LP A+   E
Sbjct: 61  EHGKVQLGNITVDMVIGGMRGMTGLLWETSLLDPEEGIRFRGLSIPECQKVLPTAQSGAE 120

Query: 121 PLPEGLLWLLLTGKVPSKEQVDALSKELQSRAVVPDYVYKAIDALPVTSHPMTQFATGVM 180
           PLPEGLLWLLLTGKVPSKEQV+ALSK+L +RA VPDYVY AIDALP T+HPMTQFA+GVM
Sbjct: 121 PLPEGLLWLLLTGKVPSKEQVEALSKDLANRAAVPDYVYNAIDALPSTAHPMTQFASGVM 180

Query: 181 GLQVQSEFQKAYEKGIHKSKYWEPTYEDSMNLIARVPLVASYVYRRIYKDGHIIPKDDSL 240
            LQVQSEFQKAYE GIHKSK+WEPTYED +NLIARVP+VA+YVYRR+YK+G  IP D SL
Sbjct: 181 ALQVQSEFQKAYENGIHKSKFWEPTYEDCLNLIARVPVVAAYVYRRMYKNGDSIPSDKSL 240

Query: 241 DYGGNFSHMLGFDSPQMQELMRLYVTIHTDHEGGNVSAHTGHLVASALSDPYLSFAAALN 300
           DYG NFSHMLGFD  +++ELMRLY+TIH+DHEGGNVSAHTGHLV SALSDPYLSFAAALN
Sbjct: 241 DYGANFSHMLGFDDEKVKELMRLYITIHSDHEGGNVSAHTGHLVGSALSDPYLSFAAALN 300

Query: 301 GLAGPLHGLANQEVLLWIKSVVDECGENITKEQLKDYVWKTLNSGKVVPGFGHGVLRKTD 360
           GLAGPLHGLANQEVLLWIKSVV+ECGE+I+KEQLK+YVWKTLNSGKV+PG+GHGVLR TD
Sbjct: 301 GLAGPLHGLANQEVLLWIKSVVEECGEDISKEQLKEYVWKTLNSGKVIPGYGHGVLRNTD 360

Query: 361 PRYTCQREFALKHLPDDPLFQL-----------------VKNPWPNVDAHSGVLLNYFGL 420
           PRY CQREFALKHLPDDPLFQL                 VKNPWPNVDAHSGVLLN++GL
Sbjct: 361 PRYVCQREFALKHLPDDPLFQLVSKLYEVVPPVLTELGKVKNPWPNVDAHSGVLLNHYGL 420

Query: 421 TEARYFTVLFGVSRSIGICSQLIWDRALGLPLERPKSVTLKWLEDHCKKAAA 456
           TEARY+TVLFGVSRS+GICSQLIWDRALGL LERPKSVT+ WLE HCKKA++
Sbjct: 421 TEARYYTVLFGVSRSLGICSQLIWDRALGLALERPKSVTMDWLEAHCKKASS 472

BLAST of CmaCh08G005600 vs. TAIR 10
Match: AT2G44350.2 (Citrate synthase family protein )

HSP 1 Score: 795.4 bits (2053), Expect = 2.4e-230
Identity = 383/473 (80.97%), Postives = 426/473 (90.06%), Query Frame = 0

Query: 1   MAFFKSLTALSKLRSRV-GQQSNLSNSVRWLQMQSSPDLDLQSHLRELIPEQQDRLKKFK 60
           M FF+S++A ++LRSRV GQQS+LSNSVRW+QMQSS DLDL+S L+ELIPEQQDRLKK K
Sbjct: 1   MVFFRSVSAFTRLRSRVQGQQSSLSNSVRWIQMQSSTDLDLKSQLQELIPEQQDRLKKLK 60

Query: 61  AEHGKVQLGNITVDMVLGGMRGMTGLLWETSLLDPDEGIRFRGLSIPECQKLLPAAKPDG 120
           +EHGKVQLGNITVDMV+GGMRGMTGLLWETSLLDP+EGIRFRGLSIPECQK+LP A+   
Sbjct: 61  SEHGKVQLGNITVDMVIGGMRGMTGLLWETSLLDPEEGIRFRGLSIPECQKVLPTAQSGA 120

Query: 121 EPLPEGLLWLLLTGKVPSKEQVDALSKELQSRAVVPDYVYKAIDALPVTSHPMTQFATGV 180
           EPLPEGLLWLLLTGKVPSKEQV+ALSK+L +RA VPDYVY AIDALP T+HPMTQFA+GV
Sbjct: 121 EPLPEGLLWLLLTGKVPSKEQVEALSKDLANRAAVPDYVYNAIDALPSTAHPMTQFASGV 180

Query: 181 MGLQVQSEFQKAYEKGIHKSKYWEPTYEDSMNLIARVPLVASYVYRRIYKDGHIIPKDDS 240
           M LQVQSEFQKAYE GIHKSK+WEPTYED +NLIARVP+VA+YVYRR+YK+G  IP D S
Sbjct: 181 MALQVQSEFQKAYENGIHKSKFWEPTYEDCLNLIARVPVVAAYVYRRMYKNGDSIPSDKS 240

Query: 241 LDYGGNFSHMLGFDSPQMQELMRLYVTIHTDHEGGNVSAHTGHLVASALSDPYLSFAAAL 300
           LDYG NFSHMLGFD  +++ELMRLY+TIH+DHEGGNVSAHTGHLV SALSDPYLSFAAAL
Sbjct: 241 LDYGANFSHMLGFDDEKVKELMRLYITIHSDHEGGNVSAHTGHLVGSALSDPYLSFAAAL 300

Query: 301 NGLAGPLHGLANQEVLLWIKSVVDECGENITKEQLKDYVWKTLNSGKVVPGFGHGVLRKT 360
           NGLAGPLHGLANQEVLLWIKSVV+ECGE+I+KEQLK+YVWKTLNSGKV+PG+GHGVLR T
Sbjct: 301 NGLAGPLHGLANQEVLLWIKSVVEECGEDISKEQLKEYVWKTLNSGKVIPGYGHGVLRNT 360

Query: 361 DPRYTCQREFALKHLPDDPLFQL-----------------VKNPWPNVDAHSGVLLNYFG 420
           DPRY CQREFALKHLPDDPLFQL                 VKNPWPNVDAHSGVLLN++G
Sbjct: 361 DPRYVCQREFALKHLPDDPLFQLVSKLYEVVPPVLTELGKVKNPWPNVDAHSGVLLNHYG 420

Query: 421 LTEARYFTVLFGVSRSIGICSQLIWDRALGLPLERPKSVTLKWLEDHCKKAAA 456
           LTEARY+TVLFGVSRS+GICSQLIWDRALGL LERPKSVT+ WLE HCKKA++
Sbjct: 421 LTEARYYTVLFGVSRSLGICSQLIWDRALGLALERPKSVTMDWLEAHCKKASS 473

BLAST of CmaCh08G005600 vs. TAIR 10
Match: AT3G60100.1 (citrate synthase 5 )

HSP 1 Score: 749.6 bits (1934), Expect = 1.5e-216
Identity = 370/466 (79.40%), Postives = 409/466 (87.77%), Query Frame = 0

Query: 1   MAFFKSLTALSKLRSRVGQQSNLSNSVRWLQMQSSPDLDLQSHLRELIPEQQDRLKKFKA 60
           M FF+S++A+S+LRSR  QQS+LSNSVRWL    S +LDL+S ++E+IPEQQDRLKK K+
Sbjct: 1   MVFFRSVSAISRLRSRAVQQSSLSNSVRWLH---SSELDLKSQMQEIIPEQQDRLKKLKS 60

Query: 61  EHGKVQLGNITVDMVLGGMRGMTGLLWETSLLDPDEGIRFRGLSIPECQKLLPAAKPDGE 120
           E GKV +GNITVDMVLGGMRGMTGLLWETSLLD DEGIRFRG+SIPECQK+LP+A+   E
Sbjct: 61  EQGKVPVGNITVDMVLGGMRGMTGLLWETSLLDADEGIRFRGMSIPECQKILPSAESGEE 120

Query: 121 PLPEGLLWLLLTGKVPSKEQVDALSKELQSRAVVPDYVYKAIDALPVTSHPMTQFATGVM 180
           PLPE LLWLLLTGKVP+KEQ +ALS EL  RA VP     AIDALP T+HPMTQFA+GVM
Sbjct: 121 PLPESLLWLLLTGKVPTKEQANALSTELAHRAAVP-----AIDALPSTAHPMTQFASGVM 180

Query: 181 GLQVQSEFQKAYEKG-IHKSKYWEPTYEDSMNLIARVPLVASYVYRRIYKDGHIIPKDDS 240
            LQVQSEFQKAYE+G I KSKYWEPT+ED++NLIARVP+VASYVYRR+YKDG IIP DDS
Sbjct: 181 ALQVQSEFQKAYEQGDISKSKYWEPTFEDALNLIARVPVVASYVYRRMYKDGSIIPLDDS 240

Query: 241 LDYGGNFSHMLGFDSPQMQELMRLYVTIHTDHEGGNVSAHTGHLVASALSDPYLSFAAAL 300
           LDYG NFSHMLGFDSPQM+ELMRLYVTIH+DHEGGNVSAH GHLV SALSDPYLSFAAAL
Sbjct: 241 LDYGANFSHMLGFDSPQMKELMRLYVTIHSDHEGGNVSAHAGHLVGSALSDPYLSFAAAL 300

Query: 301 NGLAGPLHGLANQEVLLWIKSVVDECGENITKEQLKDYVWKTLNSGKVVPGFGHGVLRKT 360
           NGLAGPLHGLANQEVLLWIK VV+ECGE+I+KEQLKDYVWKTLNSGKVVPG+GHGVLRKT
Sbjct: 301 NGLAGPLHGLANQEVLLWIKLVVEECGESISKEQLKDYVWKTLNSGKVVPGYGHGVLRKT 360

Query: 361 DPRYTCQREFALKHLPDDPLFQL-----------------VKNPWPNVDAHSGVLLNYFG 420
           DPRY CQREFALKHLPDDPLFQL                 VKNPWPNVDAHSGVLLNY+G
Sbjct: 361 DPRYICQREFALKHLPDDPLFQLVSKLYEVVPPILTELGKVKNPWPNVDAHSGVLLNYYG 420

Query: 421 LTEARYFTVLFGVSRSIGICSQLIWDRALGLPLERPKSVTLKWLED 449
           LTEARY+TVLFGVSRS+GICSQLIWDRALGLPLERPKSV + WL++
Sbjct: 421 LTEARYYTVLFGVSRSLGICSQLIWDRALGLPLERPKSVNMDWLDN 458

BLAST of CmaCh08G005600 vs. TAIR 10
Match: AT2G42790.1 (citrate synthase 3 )

HSP 1 Score: 124.8 bits (312), Expect = 1.8e-28
Identity = 105/392 (26.79%), Postives = 175/392 (44.64%), Query Frame = 0

Query: 90  SLLDPDEGI-RFRGLSIPECQKLLPAAKPDGEPLPEGLLWLLLTGKVPSKEQVDALSKEL 149
           S +D DEGI R+RG  I E  +     +         + +LL+ G +PS+ Q+      +
Sbjct: 105 SYIDGDEGILRYRGYPIEEMAENSTFLE---------VAYLLMYGNLPSESQLSDWEFAV 164

Query: 150 QSRAVVPDYVYKAIDALPVTSHPMTQFATGVMGLQVQSEFQKAYEKG--IHKSKYWEPTY 209
              + VP  V   I ++P  +HPM    + +  L +         +G  I+ SK  +   
Sbjct: 165 SQHSAVPQGVLDIIQSMPHDAHPMGVLVSAMSALSIFHPDANPALRGQDIYDSK--QVRD 224

Query: 210 EDSMNLIARVPLVASYVYRRIYKDGHIIPKDDSLDYGGNFSHMLGF-------DSPQMQE 269
           +  + +I + P +A+  Y R+     ++P   +L Y  NF +ML          +P++  
Sbjct: 225 KQIIRIIGKAPTIAAAAYLRMAGRPPVLP-SGNLPYADNFLYMLDSLGNRSYKPNPRLAR 284

Query: 270 LMRLYVTIHTDHEGGNVSAHTGHLVASALSDPYLSFAAALNGLAGPLHGLANQEVLLWIK 329
           ++ +   +H +HE    +A   HL +S + D Y + A A+  L GPLHG AN+ VL    
Sbjct: 285 VLDILFILHAEHEMNCSTAAARHLASSGV-DVYTAVAGAVGALYGPLHGGANEAVL---- 344

Query: 330 SVVDECGENITKEQLKDYVWKTLNSGKVVPGFGHGVLRKTDPRYTCQREFA---LKHLPD 389
            ++ E G   T E + +++    N  + + GFGH V +  DPR    +  A      +  
Sbjct: 345 KMLSEIG---TVENIPEFIEGVKNRKRKMSGFGHRVYKNYDPRAKVIKNLADEVFSIVGK 404

Query: 390 DPLFQLV---------------KNPWPNVDAHSGVLLNYFGLTEARYFTVLFGVSRSIGI 449
           DPL ++                +  +PNVD +SG++    G     +FTVLF + R  G 
Sbjct: 405 DPLIEVAVALEKAALSDDYFVKRKLYPNVDFYSGLIYRAMGF-PPEFFTVLFAIPRMAGY 464

BLAST of CmaCh08G005600 vs. TAIR 10
Match: AT3G58750.1 (citrate synthase 2 )

HSP 1 Score: 120.2 bits (300), Expect = 4.4e-27
Identity = 100/371 (26.95%), Postives = 165/371 (44.47%), Query Frame = 0

Query: 92  LDPDEGI-RFRGLSIPECQKLLPAAKPDGEPLPEGLLWLLLTGKVPSKEQVDALSKELQS 151
           +D DEGI R+RG  I E  +     +         + +LL+ G +PS+ Q+      +  
Sbjct: 112 IDGDEGILRYRGYPIEELAESSTFIE---------VAYLLMYGNLPSQSQLADWEFTVSQ 171

Query: 152 RAVVPDYVYKAIDALPVTSHPMTQFATGVMGLQVQSEFQKAYEKG--IHKSKYWEPTYED 211
            + VP  V   I ++P  +HPM    + +  L +          G  I+KSK  +   + 
Sbjct: 172 HSAVPQGVLDIIQSMPHDAHPMGVLVSAMSALSIFHPDANPALSGQDIYKSK--QVRDKQ 231

Query: 212 SMNLIARVPLVASYVYRRIYKDGHIIPKDDSLDYGGNFSHMLGF-------DSPQMQELM 271
            + ++ + P +A+  Y R      ++P   +L Y  NF +ML          +P++  ++
Sbjct: 232 IVRILGKAPTIAAAAYLRTAGRPPVLP-SANLSYSENFLYMLDSMGNRSYKPNPRLARVL 291

Query: 272 RLYVTIHTDHEGGNVSAHTGHLVASALSDPYLSFAAALNGLAGPLHGLANQEVLLWIKSV 331
            +   +H +HE    +A   HL +S + D Y + A A+  L GPLHG AN+ VL     +
Sbjct: 292 DILFILHAEHEMNCSTAAARHLASSGV-DVYTACAGAVGALYGPLHGGANEAVL----KM 351

Query: 332 VDECGENITKEQLKDYVWKTLNSGKVVPGFGHGVLRKTDPRYTCQREFA---LKHLPDDP 391
           + E G   T E + D++    N  + + GFGH V +  DPR    ++ A      +  DP
Sbjct: 352 LAEIG---TAENIPDFIEGVKNRKRKMSGFGHRVYKNYDPRAKVIKKLADEVFSIVGRDP 411

Query: 392 LFQLV---------------KNPWPNVDAHSGVLLNYFGLTEARYFTVLFGVSRSIGICS 435
           L ++                +  +PNVD +SG++    G     +FTVLF V R  G  S
Sbjct: 412 LIEVAVALEKAALSDEYFVKRKLYPNVDFYSGLIYRAMGF-PPEFFTVLFAVPRMAGYLS 459

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P492983.5e-23985.56Citrate synthase, mitochondrial OS=Citrus maxima OX=37334 GN=CIT PE=2 SV=1[more]
O804335.8e-23482.42Citrate synthase, mitochondrial OS=Daucus carota OX=4039 GN=CS PE=2 SV=1[more]
P201153.3e-22980.97Citrate synthase 4, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=CSY4 PE=1 S... [more]
Q431753.8e-21779.03Citrate synthase, mitochondrial OS=Solanum tuberosum OX=4113 PE=2 SV=1[more]
Q9M1D32.1e-21579.40Citrate synthase 5, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=CSY5 PE=1 S... [more]
Match NameE-valueIdentityDescription
AT2G44350.19.6e-23281.14Citrate synthase family protein [more]
AT2G44350.22.4e-23080.97Citrate synthase family protein [more]
AT3G60100.11.5e-21679.40citrate synthase 5 [more]
AT2G42790.11.8e-2826.79citrate synthase 3 [more]
AT3G58750.14.4e-2726.95citrate synthase 2 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002020Citrate synthasePRINTSPR00143CITRTSNTHASEcoord: 387..403
score: 55.43
coord: 283..311
score: 61.14
coord: 212..225
score: 44.51
coord: 260..275
score: 47.24
coord: 408..422
score: 53.72
IPR002020Citrate synthasePFAMPF00285Citrate_syntcoord: 78..439
e-value: 3.6E-93
score: 312.5
IPR002020Citrate synthasePANTHERPTHR11739CITRATE SYNTHASEcoord: 1..455
IPR016143Citrate synthase-like, small alpha subdomainGENE3D1.10.230.10coord: 310..407
e-value: 9.1E-180
score: 599.7
IPR010109Citrate synthase, eukaryotic-typeTIGRFAMTIGR01793TIGR01793coord: 38..446
e-value: 3.4E-229
score: 758.7
IPR016142Citrate synthase-like, large alpha subdomainGENE3D1.10.580.10Citrate Synthase, domain 1coord: 44..439
e-value: 9.1E-180
score: 599.7
NoneNo IPR availablePANTHERPTHR11739:SF33CITRATE SYNTHASE 4, MITOCHONDRIALcoord: 1..455
IPR019810Citrate synthase active sitePROSITEPS00480CITRATE_SYNTHASEcoord: 350..362
IPR036969Citrate synthase superfamilySUPERFAMILY48256Citrate synthasecoord: 42..450

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh08G005600.1CmaCh08G005600.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
biological_process GO:0006101 citrate metabolic process
biological_process GO:0006099 tricarboxylic acid cycle
cellular_component GO:0005759 mitochondrial matrix
molecular_function GO:0004108 citrate (Si)-synthase activity
molecular_function GO:0046912 acyltransferase, acyl groups converted into alkyl on transfer