CmaCh08G005360 (gene) Cucurbita maxima (Rimu) v1.1

BLAST of CmaCh08G005360 vs. ExPASy Swiss-Prot
Match: E0X9N4 (Putative RING-type E3 ubiquitin transferase C3H69 OS=Arabidopsis thaliana OX=3702 GN=At3g63550 PE=3 SV=1)

HSP 1 Score: 486.9 bits (1252), Expect = 2.0e-136
Identity = 233/359 (64.90%), Postives = 286/359 (79.67%), Query Frame = 0

Query: 1   MSKRVLCKYFAHGACLKGEYCEFSHDWTAPPSNVCTYYQKGRCAFGSRCRYDHVKASHAE 60
           MSKR+LCK+FAHGACLKGE+CEFSHDW  P +N+CTYYQKG C++GSRCRY+HVKAS   
Sbjct: 1   MSKRILCKFFAHGACLKGEHCEFSHDWKDPTNNICTYYQKGICSYGSRCRYEHVKASRPH 60

Query: 61  SSASSNSQQPHMSESVSIAHTLLSSPLSPSPGSTIELAGSKISCLP-SHVESAWSVECEL 120
            SASS+S  P  S + S ++TL  + L   PG    +A   +S +P S ++  W+++   
Sbjct: 61  PSASSSSAVPRPSPA-SASNTLACAFL---PG----VAERDLSPVPSSSLKPTWNLDSLH 120

Query: 121 PSGLDDWGSRETRIISPADLPICSFSAAGTNCPRGDNCPHIHGDLCPTCGKQCLHPFRTD 180
              +D+  +     + P +LPICSF+AAG +CPRG+ CPHIHG +CPTCGK CLHPFR D
Sbjct: 121 QDSVDEVKTSNPGTVKPEELPICSFAAAG-DCPRGNECPHIHGTICPTCGKCCLHPFRPD 180

Query: 181 EREEHMKACEKKQKHLESLKHSQEIECSVCLERVLSKTEIAERKFGLLSECDHPFCISCI 240
           EREEH K CEKK K LE+LK SQEIEC VCLERVLSK   AERKFGLL+ECDH FCI+CI
Sbjct: 181 EREEHKKVCEKKHKQLEALKLSQEIECCVCLERVLSKATPAERKFGLLTECDHAFCIACI 240

Query: 241 RNWRNSSSTSGMDVNSALRACPICRKLSYFVIPSVIWYSSKEEKQEIVDSYKSKLRSIDC 300
           RNWR+SS ++GMDVNS LRACPICRKLSYFV+PSVIW+S+ EEK+EI+D+Y+ KLRSIDC
Sbjct: 241 RNWRSSSPSTGMDVNSTLRACPICRKLSYFVVPSVIWFSAPEEKKEIMDNYREKLRSIDC 300

Query: 301 KHFNFGNGNCPFGSSCFYKHAHRDGRLEEVALRHIGNQDGLTIIAKNIRLSDFLSDLHI 359
           KHF+FG+GNCPFG+SCFYKHA+ DGRLEEV LRH+  +DG T+IAK+IRLSDFL ++HI
Sbjct: 301 KHFSFGDGNCPFGTSCFYKHAYHDGRLEEVVLRHLDAEDGQTVIAKDIRLSDFLENMHI 350

BLAST of CmaCh08G005360 vs. ExPASy Swiss-Prot
Match: Q5ZA07 (E3 ubiquitin-protein ligase makorin OS=Oryza sativa subsp. japonica OX=39947 GN=MKRN PE=2 SV=1)

HSP 1 Score: 476.5 bits (1225), Expect = 2.7e-133
Identity = 238/368 (64.67%), Postives = 269/368 (73.10%), Query Frame = 0

Query: 2   SKRVLCKYFAHGACLKGEYCEFSHDWTAPPSNVCTYYQKGRCAFGSRCRYDHVKASHAES 61
           +KRVLCK+F HGACLKGEYCEFSHDW   P+NVCT+YQKG C++GSRCRYDHVK S   +
Sbjct: 3   TKRVLCKFFMHGACLKGEYCEFSHDWNDQPNNVCTFYQKGSCSYGSRCRYDHVKVSRNPT 62

Query: 62  SASSNSQQPHMSESVSIAHTLLSSPL-------SPSPGSTIELAGSKISCLPSHVESAWS 121
            A   S       S S+       P        S +P   I +     S       + + 
Sbjct: 63  VAPPPSSSTTTRASSSLQPLSFGRPHHVGYQADSSNPRQQISMDVLAHSGSKPVWRNDFQ 122

Query: 122 VECELPSGLDDWGSRET----RIISPADLPICSFSAAGTNCPRGDNCPHIHGDLCPTCGK 181
            E  L  G+ DW    T      +SPADLPICSF AAG NCP G+ CP +HGDLC TCGK
Sbjct: 123 HESVLEDGI-DWSISPTVQNQTTLSPADLPICSF-AAGGNCPYGEECPQMHGDLCTTCGK 182

Query: 182 QCLHPFRTDEREEHMKACEKKQKHLESLKHSQEIECSVCLERVLSKTEIAERKFGLLSEC 241
            CLHP+R DEREEH K CEK  K LESLK SQEIECSVCL+RVLSK   AERKFGLLSEC
Sbjct: 183 MCLHPYRPDEREEHTKLCEKNHKRLESLKRSQEIECSVCLDRVLSKPTAAERKFGLLSEC 242

Query: 242 DHPFCISCIRNWRNSSSTSGMDVNSALRACPICRKLSYFVIPSVIWYSSKEEKQEIVDSY 301
           DHPFCISCIRNWRN+S TSGMDVNSALRACPICRKLSY+VIPSV+WY SKEEK EI+D+Y
Sbjct: 243 DHPFCISCIRNWRNNSPTSGMDVNSALRACPICRKLSYYVIPSVLWYFSKEEKLEIIDNY 302

Query: 302 KSKLRSIDCKHFNFGNGNCPFGSSCFYKHAHRDGRLEEVALRHIGNQDGLTIIAKNIRLS 359
           K+KL+SIDCK+F+FG G CPFGSSCFYKHA+RDGRLEEV LRH+   DG T+IAKNIRLS
Sbjct: 303 KAKLKSIDCKYFDFGTGTCPFGSSCFYKHAYRDGRLEEVILRHLDADDGSTVIAKNIRLS 362

BLAST of CmaCh08G005360 vs. ExPASy Swiss-Prot
Match: Q6IDS6 (E3 ubiquitin-protein ligase makorin OS=Arabidopsis thaliana OX=3702 GN=MKRN PE=2 SV=1)

HSP 1 Score: 453.4 bits (1165), Expect = 2.4e-126
Identity = 219/358 (61.17%), Postives = 264/358 (73.74%), Query Frame = 0

Query: 1   MSKRVLCKYFAHGACLKGEYCEFSHDWTAPPSNVCTYYQKGRCAFGSRCRYDHVKASHAE 60
           MS R+LCK+F HG+CLKGE CEFSHD   PP+NVCT+YQK  C +GSRCRYDHV+A+   
Sbjct: 1   MSDRILCKFFVHGSCLKGENCEFSHDSKDPPNNVCTFYQKRICLYGSRCRYDHVRAA--- 60

Query: 61  SSASSNSQQPHMSESVSIAHTLLSSPLSPSPGSTIELAGSKISCLPSHVESAWSVECELP 120
                 S  P  S+S S     L   +S +P   ++  G       +  + + +V C   
Sbjct: 61  ------SNLPLSSDSES-----LDRSISTTPSRHLQQQGDN-----NDGDKSSNVYC--- 120

Query: 121 SGLDDWGSRETRIISPADLPICSFSAAGTNCPRGDNCPHIHGDLCPTCGKQCLHPFRTDE 180
                        I P + PICSF+AAG +CPRG+ CPH+HGDLC TCGK+CLHPFR +E
Sbjct: 121 -------------IHPREYPICSFAAAG-DCPRGNQCPHMHGDLCNTCGKKCLHPFRPEE 180

Query: 181 REEHMKACEKKQKHLESLKHSQEIECSVCLERVLSKTEIAERKFGLLSECDHPFCISCIR 240
           REEH K CEKKQKH+E+LK SQ+IECSVCL+R+LSK    ERKFGLL+ECDHPFCI CIR
Sbjct: 181 REEHTKECEKKQKHIEALKQSQDIECSVCLDRILSKATPGERKFGLLTECDHPFCIQCIR 240

Query: 241 NWRNSSSTSGMDVNSALRACPICRKLSYFVIPSVIWYSSKEEKQEIVDSYKSKLRSIDCK 300
           NWR+S+  SGMDVNS LRACPICRKLSYFV+PSV+WYSS EEK+EI+D YK+KLRSIDCK
Sbjct: 241 NWRSSAPVSGMDVNSTLRACPICRKLSYFVVPSVVWYSSPEEKKEIIDIYKAKLRSIDCK 300

Query: 301 HFNFGNGNCPFGSSCFYKHAHRDGRLEEVALRHIGNQDGLTIIAKNIRLSDFLSDLHI 359
           HFNFGNGNCPFG+SCFYKHA+ DG LEEV LRH+G+Q+G T+I  +IRLS+FL  L I
Sbjct: 301 HFNFGNGNCPFGASCFYKHAYSDGHLEEVVLRHLGSQEGETVITDSIRLSEFLGGLQI 322

BLAST of CmaCh08G005360 vs. ExPASy Swiss-Prot
Match: Q9ERV1 (Probable E3 ubiquitin-protein ligase makorin-2 OS=Mus musculus OX=10090 GN=Mkrn2 PE=1 SV=2)

HSP 1 Score: 243.0 bits (619), Expect = 5.0e-63
Identity = 144/399 (36.09%), Postives = 206/399 (51.63%), Query Frame = 0

Query: 2   SKRVLCKYFAHGACLKGEYCEFSHDW-TAPPSNVCTYYQKGRCAFGSRCRYDHVKASHAE 61
           +K+V C+YF HG C +G  C FSHD   + PS +C YYQKG CA+G+RCRYDH K   A 
Sbjct: 3   TKQVTCRYFMHGVCREGSQCLFSHDLANSKPSTICKYYQKGYCAYGARCRYDHTKPPAAA 62

Query: 62  SSA---------SSNSQQPHMSESVSIAHTLLSSPLSPSPG----STIELAGSKISCL-- 121
             A         SS    PH S  ++   T +    S  PG     T+ L    ++ L  
Sbjct: 63  GGAVGPAPNPSPSSGLHSPHPSPDIA---TSVMRTHSNEPGKREKKTLVLRDRNLTGLAE 122

Query: 122 ----PSHVESAWSVECELP----------------SGLDDWGSRETRIISPADLPICSFS 181
               PS V +     C  P                +GLDD  +  +    P    +C ++
Sbjct: 123 DKTPPSKVNNPGG--CSDPQTSPEMKPHSYLDAIRTGLDDLEASSSYSNEP---QLCPYA 182

Query: 182 AAGTNCPRGDNCPHIHGDLCPTCGKQCLHPFRTDEREEHMKAC----EKKQKHLESLKHS 241
           AAG  C  GD C ++HGD+C  C  Q LHPF  ++R+ H K C    E + +   + + S
Sbjct: 183 AAG-ECRFGDACVYLHGDMCEICRLQVLHPFDPEQRKAHEKMCMSTFEHEMEKAFAFQAS 242

Query: 242 QEIECSVCLERVLSKTEIAERKFGLLSECDHPFCISCIRNWRNSSSTSGMDVNSALRACP 301
           Q+  CS+C+E +L K   +ER+FG+LS C H +C+SCIR WR +        N  +++CP
Sbjct: 243 QDKVCSICMEVILEKASASERRFGILSNCSHTYCLSCIRQWRCAKQFE----NPIIKSCP 302

Query: 302 ICRKLSYFVIPSVIWYSSKEEKQEIVDSYKSKLRSIDCKHFNFGNGNCPFGSSCFYKHAH 360
            CR +S FVIPSV W   + +K E+++++K  +    CK+F  G G CPFGS C Y+HA+
Sbjct: 303 ECRVISEFVIPSVYWVEDQNKKNELIEAFKQGMGKKACKYFEQGKGTCPFGSKCLYRHAY 362

BLAST of CmaCh08G005360 vs. ExPASy Swiss-Prot
Match: Q4VBT5 (Probable E3 ubiquitin-protein ligase makorin-1 OS=Danio rerio OX=7955 GN=mkrn1 PE=2 SV=1)

HSP 1 Score: 241.9 bits (616), Expect = 1.1e-62
Identity = 132/346 (38.15%), Postives = 192/346 (55.49%), Query Frame = 0

Query: 2   SKRVLCKYFAHGACLKGEYCEFSHDWTA-PPSNVCTYYQKGRCAFGSRCRYDHVKASHAE 61
           +K V C+YF HG C +GE C +SHD ++   + +C ++QKG CAFG RCRY+H K S  +
Sbjct: 19  TKHVTCRYFMHGLCKEGENCRYSHDLSSCKQTMICKFFQKGCCAFGDRCRYEHTKPSKQD 78

Query: 62  SSASSNSQQPHMSESVSIAHTLLSSPLSPSPGSTIEL--------------AGSKISCLP 121
              SS    P    +  +A T    P+S  PG T                 A   +   P
Sbjct: 79  EVPSSKPSMP--LTAAPLAGT--PEPVSDGPGGTTGAQEKPQGSGAVDWVNAAEFVPGQP 138

Query: 122 SHVESAWSVECELPSGLDDWGSRETRIISPADLPICSFSAAGTNCPRGDNCPHIHGDLCP 181
            +   A  V CE P  L +    + +        +C ++A G  C  G NC ++HGD+C 
Sbjct: 139 -YCGRADPVLCEGPGPLIEEEYEKEQANKEMKKQLCPYAAVG-ECRYGLNCAYLHGDVCD 198

Query: 182 TCGKQCLHPFRTDEREEHMKAC-EKKQKHLE---SLKHSQEIECSVCLERVLSKTEIAER 241
            CG Q LHP  T +R +H++AC E  +K +E   +++ S+++ C VC+E V  KT  +ER
Sbjct: 199 MCGLQVLHPSDTSQRSQHIRACIEAHEKDMEISFAIQRSKDMMCGVCMEVVFEKTNPSER 258

Query: 242 KFGLLSECDHPFCISCIRNWRNSSSTSGMDVNSALRACPICRKLSYFVIPSVIWYSSKEE 301
           +FG+LS C H +C+ CIR WR++        +  +++CP CR  S FVIPS  W   KEE
Sbjct: 259 RFGILSNCCHCYCLKCIRKWRSAKQFE----SKIIKSCPECRITSNFVIPSEYWVEDKEE 318

Query: 302 KQEIVDSYKSKLRSIDCKHFNFGNGNCPFGSSCFYKHAHRDGRLEE 329
           KQ+++  YK  + +  C++F+ G G CPFG++CFYKHA  DGRLEE
Sbjct: 319 KQQLIQKYKDGMGTKPCRYFDEGRGTCPFGANCFYKHAFPDGRLEE 354

BLAST of CmaCh08G005360 vs. TAIR 10
Match: AT3G08505.2 (zinc finger (CCCH-type/C3HC4-type RING finger) family protein )

HSP 1 Score: 453.4 bits (1165), Expect = 1.7e-127
Identity = 219/358 (61.17%), Postives = 264/358 (73.74%), Query Frame = 0

Query: 1   MSKRVLCKYFAHGACLKGEYCEFSHDWTAPPSNVCTYYQKGRCAFGSRCRYDHVKASHAE 60
           MS R+LCK+F HG+CLKGE CEFSHD   PP+NVCT+YQK  C +GSRCRYDHV+A+   
Sbjct: 1   MSDRILCKFFVHGSCLKGENCEFSHDSKDPPNNVCTFYQKRICLYGSRCRYDHVRAA--- 60

Query: 61  SSASSNSQQPHMSESVSIAHTLLSSPLSPSPGSTIELAGSKISCLPSHVESAWSVECELP 120
                 S  P  S+S S     L   +S +P   ++  G       +  + + +V C   
Sbjct: 61  ------SNLPLSSDSES-----LDRSISTTPSRHLQQQGDN-----NDGDKSSNVYC--- 120

Query: 121 SGLDDWGSRETRIISPADLPICSFSAAGTNCPRGDNCPHIHGDLCPTCGKQCLHPFRTDE 180
                        I P + PICSF+AAG +CPRG+ CPH+HGDLC TCGK+CLHPFR +E
Sbjct: 121 -------------IHPREYPICSFAAAG-DCPRGNQCPHMHGDLCNTCGKKCLHPFRPEE 180

Query: 181 REEHMKACEKKQKHLESLKHSQEIECSVCLERVLSKTEIAERKFGLLSECDHPFCISCIR 240
           REEH K CEKKQKH+E+LK SQ+IECSVCL+R+LSK    ERKFGLL+ECDHPFCI CIR
Sbjct: 181 REEHTKECEKKQKHIEALKQSQDIECSVCLDRILSKATPGERKFGLLTECDHPFCIQCIR 240

Query: 241 NWRNSSSTSGMDVNSALRACPICRKLSYFVIPSVIWYSSKEEKQEIVDSYKSKLRSIDCK 300
           NWR+S+  SGMDVNS LRACPICRKLSYFV+PSV+WYSS EEK+EI+D YK+KLRSIDCK
Sbjct: 241 NWRSSAPVSGMDVNSTLRACPICRKLSYFVVPSVVWYSSPEEKKEIIDIYKAKLRSIDCK 300

Query: 301 HFNFGNGNCPFGSSCFYKHAHRDGRLEEVALRHIGNQDGLTIIAKNIRLSDFLSDLHI 359
           HFNFGNGNCPFG+SCFYKHA+ DG LEEV LRH+G+Q+G T+I  +IRLS+FL  L I
Sbjct: 301 HFNFGNGNCPFGASCFYKHAYSDGHLEEVVLRHLGSQEGETVITDSIRLSEFLGGLQI 322

BLAST of CmaCh08G005360 vs. TAIR 10
Match: AT3G08505.1 (zinc finger (CCCH-type/C3HC4-type RING finger) family protein )

HSP 1 Score: 453.4 bits (1165), Expect = 1.7e-127
Identity = 219/358 (61.17%), Postives = 264/358 (73.74%), Query Frame = 0

Query: 1   MSKRVLCKYFAHGACLKGEYCEFSHDWTAPPSNVCTYYQKGRCAFGSRCRYDHVKASHAE 60
           MS R+LCK+F HG+CLKGE CEFSHD   PP+NVCT+YQK  C +GSRCRYDHV+A+   
Sbjct: 1   MSDRILCKFFVHGSCLKGENCEFSHDSKDPPNNVCTFYQKRICLYGSRCRYDHVRAA--- 60

Query: 61  SSASSNSQQPHMSESVSIAHTLLSSPLSPSPGSTIELAGSKISCLPSHVESAWSVECELP 120
                 S  P  S+S S     L   +S +P   ++  G       +  + + +V C   
Sbjct: 61  ------SNLPLSSDSES-----LDRSISTTPSRHLQQQGDN-----NDGDKSSNVYC--- 120

Query: 121 SGLDDWGSRETRIISPADLPICSFSAAGTNCPRGDNCPHIHGDLCPTCGKQCLHPFRTDE 180
                        I P + PICSF+AAG +CPRG+ CPH+HGDLC TCGK+CLHPFR +E
Sbjct: 121 -------------IHPREYPICSFAAAG-DCPRGNQCPHMHGDLCNTCGKKCLHPFRPEE 180

Query: 181 REEHMKACEKKQKHLESLKHSQEIECSVCLERVLSKTEIAERKFGLLSECDHPFCISCIR 240
           REEH K CEKKQKH+E+LK SQ+IECSVCL+R+LSK    ERKFGLL+ECDHPFCI CIR
Sbjct: 181 REEHTKECEKKQKHIEALKQSQDIECSVCLDRILSKATPGERKFGLLTECDHPFCIQCIR 240

Query: 241 NWRNSSSTSGMDVNSALRACPICRKLSYFVIPSVIWYSSKEEKQEIVDSYKSKLRSIDCK 300
           NWR+S+  SGMDVNS LRACPICRKLSYFV+PSV+WYSS EEK+EI+D YK+KLRSIDCK
Sbjct: 241 NWRSSAPVSGMDVNSTLRACPICRKLSYFVVPSVVWYSSPEEKKEIIDIYKAKLRSIDCK 300

Query: 301 HFNFGNGNCPFGSSCFYKHAHRDGRLEEVALRHIGNQDGLTIIAKNIRLSDFLSDLHI 359
           HFNFGNGNCPFG+SCFYKHA+ DG LEEV LRH+G+Q+G T+I  +IRLS+FL  L I
Sbjct: 301 HFNFGNGNCPFGASCFYKHAYSDGHLEEVVLRHLGSQEGETVITDSIRLSEFLGGLQI 322