CmaCh08G004990 (gene) Cucurbita maxima (Rimu) v1.1

Overview


Name	CmaCh08G004990
Type	gene
Organism	Cucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Description	Glutamine synthetase
Location	Cma_Chr08: 2840165 .. 2844928 (-)
RNA-Seq Expression	CmaCh08G004990
Synteny	CmaCh08G004990

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AGTCCCCTATTAAACCCCTCACGTCCAGCCGCCTTCTCTCTCACCAAGCACCATTTCATTTCAACGCTTCTCTCTCTCTCTCTCTCTCTCTCTCGCTCCCGCTTTTTCCTCTGCTTCACTCGCCATGTCCCTCCTCTCTGACCTCATCAATCTCAACCTCACTGATTCTACCGACAAGATCATTGCCGAATACGTATGGTAATTGTTTTCCTCTCTTCTTCGCTTGCTCTGTTCTCTTTTCATGCTTCTTTGCGGATTTGTTCATTTTTTGTTTTGTTTCTTGCAGGATTGGAGGATCGGGGCTCGATTTGAGAAGCAAAGCTAGGGTATGGACTGCTTTTTTCTTTTTCTTCTCGTGTTTGTTCGGCGTTTGGATCAATATCTGATTGGTTTCGCTGTTTGTGATATTGATTAATTAGTTGATCTCTGTTTTTTGTTCGAGAGCTTGGAATCGATTGCGTGTGTGTGTGTATGTTCTAAGTTTTTCTGGAGAGATTTCTTGATGTTTCAACTCGTTTCGAAATTTGTTGGTGCTTATAATTTTTCTTCAGATTTCGAATGTTCTTACCTCTGATCATGTATTTTTATTTTTAATTTTTTGCCTGTTTCTAATCGTTCGGGAAATTATCGGTACAGATCATATATACAGATTTCAAGTGTTTCTCAGTTTTTTTCTTTTTCTTTTTTTTTTCTTTTTTCTTTCTTCGACTTTTTCTTTGAAGTTCGGAATCGATCATACTTTTGAGGAGTTGTTCAGGAGAGATTTTCATGCTGTATCGACTTGTTTGAGAAATTAGTAGAGTACGGCACACGGCATTTCTTCAAATTTAGAGTGTTTGTCCCTCTTTTTTTAAAAGATTTCTTCAACTTTTCACTTGCGAGTTCAAAATTGGTTGTGCATTTGGATAGTTTTCTGGAGAGATTTTCTTGCCGCATCTACTCGTTTAAGAAATTTTCAGTACAGTACGGATCATTATTCAGATTTCGAATGTATCTCTTCTTTTTTCGTTCGTCGACGTTTTGTTTGAGAGTTGAAAATCGGTCGTTCTTCTGCAAAGCTTTTCCGTTCATATTTCTTGCTGTGTCCGTACAGATCGTTCCTTAGGATTCTAAATGACATTTTAAAAACTTCTTCAGACTCTTCCGGGACTGGTGACGGATCCAGCGAAGCTCCCGAGGTGGAACTACGACGGTTCCAGTACGAATCAAGCCCCCGGAGACGACAGCGAAGTCATCTTATAGTATGTACAACGCCCCCACCCTATTACCATACGCCACCTGTCCTTACCGATGAAGATTCTACACGGACTGTAGAATCTTTGACTTTTTTGCTCTTATCTGAAATATCCGCATTTAACGGCGTTAGTCTTGTTTGACTATTAACAGTCCCCAAGCTATTTTCAAGGATCCATTCAGAAGAGGAAACAACATCCTGGTATGGTGAATTCACCGGTGTATTTTGGTATTTTCCAAGTGAATACAAAGGGTAATTTCGTCTTTTCGTTTGATAATGACGTCGACATAGGTTATGTGCGATGCTTATACGCCATCTGGCGAGCCGATTCCGACGAACAAGAGATTCAACGCCGCCAAGATTTTTAGCCACGCCGACGTCGTTGCGGAGGAACCTTGGTAATCCTATTAATTACTTTAAAAAAATGACTTTCTTATTCCCTTTACTTATAAAAAGAATGAGAATAGGAATATAAAAATTATTTTAATCAGGAATTATATTCGAATCTCGTTATTTTCTATATTTATATATTCCTGTTTTGGGACGAATTTCACATTTAATGTAAACTAATTAACCTAACCACTAATATTCCCATTTAATAAATGGGTTGTGTTTCACATTTCAAAATTTCAAATTATCAAAAAACTTTGAAAAGTAATCTAAAAAGTTATTTCAAATCCTATACCTAATCTTCTGCTTTTTCCATTTTTAAAGACATTCATGGACACCTCTCCACTCCCCTTGGGCTTGGAACTTGGGAACTGTGACCTTTCTCATGAGTGGGGTTGGTGTTCAAATATAAAATTGGTAGTTGTCAAGAGGGTGAGATAAAATGTCGTATTGGATCAGTTATTAAAATGCGTCCGCCGTCTGCTTTTGACTGGAACTTCTCGCTACCATGAAATTCGTTGGTTTAATAATTGTATTAGGTATGAAATTTGCAATTGTGCGTATTAAATTAACTGAAAAAGTTTAGGACCTAAAAGGCAATGGTGTCCCATAACAGCAAAAGCTGGATGTTAGGCCTCGCCATGTATGCTTCCGTACACATTAATTTATTTTATCACATTTCAGCACTAAAATTACGCACTTAGATTTTTCACGTGCTCCACTTTCACATCATATTTTTCTTTTATGTTTCTCTTATGCTAAATGATATGTTTTTTTGTTGATTAATTTATGAATTTTAACTATAAGTAAATGGATTATGGATATAGACACAGAGATTATTGATTCAATGGTATGATTCATTTTAAGATGTGTTTTGATGGTGATGACAGGTATGGGATTGAGCAGGAGTACACTCTCCTCCAAAAGGACGTCCACTGGCCACTCGGTTGGCCTGTAGGTGGCTTCCCAGGCCCACAGGTACCCTTAATGCTGTTTAATCATCATCATTAAAGCTGCTTCTCTTCACCGACTGTGTAGATATTTTAATGAATAGTTCTTTCATAACCAAAAAATGAGGGTCGGAGAAACAAAATTCAAACAATTTCAAGTCGACTGGGCAGAGTTTTTATTGGTCTATTATTATTAAGAACAATTTTCGTTTATCAATAAGATAATCTACTAAAATATTAGTAAACTTGTGGAAAGAGGACAAGCAGTTTTTAACAATAATGATTTAAAAATAAATAAATAATAAGTATATACCCAAAGTGATGTCAGATCAGCATTGCTTCTAGAAATAGTGTAAGAAAAATGGTTATTAAACAATAGCTAAGGCTTGTAGTTTTAGTAAAAACTATTTTAGGACCTCGAGTTTCGTTTACTCAAAATTATCTGTTTAATTGGAATGTGTACAAAAATGGTTTGGTAAAAAGAATCCTAAATGTTAGATAATTATCTGAATCGGTGCCATGCAGGGTCCTTACTATTGTGGCACAGGAGCAGACAAGGCCTTCGGTCGAGATATTGTAGACGCTCACTATAAGGCTTGTCTTTATGCCGGAGTCAATATCAGTGGAATCAACGGTGAAGTCATGCCCGGTCAGGTGCGTCACCATCGTAATATCCAGTGTCTAAGCACACAGATAATATCCGATGTTATTTATTTATTCTCTCCTTAAAATGATTCCCATTTTTTTGTTTTGTTCTGTTCTGTTTGGACAGTGGGAATACCAAGTCGGTCCAACCGTTGGTATTGCTGCTGGTGATCAAGTTTGGATCTCCCGATACATCCTCGAGGTACACTCTAATTCCAATATGAAAAATCACCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTGAACAATGACTAATCTCCCAGGTTAGAGTTCATTTTGAGCATAAGCACATATGATTTTGGTTTTGGTTTCTTTAAAAGGTCTAATTTTAATAGATATGTATTCCTTATTTAGTGGAACTTAATTAGCTGGTGGGACTCCACTCCCTAACTTACTTTTTCTATTTTTTTTATTTTTTTGTGGTGGTGAACAGAGAATCACAGAAATTGCAGGAGTTGTTCTGTCCTTCGACCCAAAGCCAATTAAGGTGTGTGATCAATTCAATCAGTAGACACCAATTAGATTGAATTATTTCGTCTTGATGGTGATTGATATGATGTTGTTTTCTTAACAACCAGGGTGATTGGAATGGCGCTGGTGCTCACACCAACTACAGGTGTTGTTTTCATCTTTCTTGAATTTGTCGTTTGTTCTTTAATAATTGAAATCTTTTTGGGTTCTTTTCTTGTGGTTGGCCTCAGCACCAAATCAATGAGAAACGACGGTGGCATTGATGTGATCAAGAAAGCCATCGAGAAACTGGGATTGCGTCATAAACTGCACATCGCTGCCTATGGAGAAGGCAACGAGAGAAGGTTAACCGGCCGCCACGAGACTGCCGATATCAACACCTTCTCTTGGGTATCTTGTCCTTTCTCCTTTACTTGCTAAACAGTTCATTGATTTACATAAAGTAGGTGAACTAATAGTGAAGAACTTACAGGGAGTTGCAAACAGAGGAGCCTCTGTCCGTGTCGGCCGCGACACTGAACAAGCCGGGAAAGGCAAGTTACTCTAAACTGAAACCATAATTTCCATTTTCCATCCTCTTTTTGCTGAAAATTTGTTCTGAAAACAATCGTTTCAGGCTACTTCGAGGATCGAAGGCCAGCTTCAAACATGGATCCTTACGTCGTCACTTCCATGATCGCCGAGACAACCATTATGTGGAAGCCGTAGACGGACTCGATCAGCCGGCGTTCTGACGGCGCCGCCCCTATCCGGTTCATATCTGTTTCTATCTACTACATTTACTGCTTCTGAGAGTGATCTATGTCCAAAACTCTGGTTTCTGTATATTTGTGTGTGCCTCGTGATTGCGTTCGGCCATTGCTTCCTGTTGTTTCCCTTTTTCTTCTTTTATCGCAAACAATAATCAATTTATTTTCTTCTGCCCCATGCTTTTATCGTTCATGCCACTAAATGTCTCTTTTTATTTTTGGGAATTAAAAATGTATTCGTGTTATTATTGTCACTTCGCATGACATAAAGTAATTGTCAGCCTTCC

mRNA sequence

AGTCCCCTATTAAACCCCTCACGTCCAGCCGCCTTCTCTCTCACCAAGCACCATTTCATTTCAACGCTTCTCTCTCTCTCTCTCTCTCTCTCTCGCTCCCGCTTTTTCCTCTGCTTCACTCGCCATGTCCCTCCTCTCTGACCTCATCAATCTCAACCTCACTGATTCTACCGACAAGATCATTGCCGAATACGTATGGATTGGAGGATCGGGGCTCGATTTGAGAAGCAAAGCTAGGACTCTTCCGGGACTGGTGACGGATCCAGCGAAGCTCCCGAGGTGGAACTACGACGGTTCCAGTACGAATCAAGCCCCCGGAGACGACAGCGAAGTCATCTTATATCCCCAAGCTATTTTCAAGGATCCATTCAGAAGAGGAAACAACATCCTGGTTATGTGCGATGCTTATACGCCATCTGGCGAGCCGATTCCGACGAACAAGAGATTCAACGCCGCCAAGATTTTTAGCCACGCCGACGTCGTTGCGGAGGAACCTTGGTATGGGATTGAGCAGGAGTACACTCTCCTCCAAAAGGACGTCCACTGGCCACTCGGTTGGCCTGTAGGTGGCTTCCCAGGCCCACAGGGTCCTTACTATTGTGGCACAGGAGCAGACAAGGCCTTCGGTCGAGATATTGTAGACGCTCACTATAAGGCTTGTCTTTATGCCGGAGTCAATATCAGTGGAATCAACGGTGAAGTCATGCCCGGTCAGAGAATCACAGAAATTGCAGGAGTTGTTCTGTCCTTCGACCCAAAGCCAATTAAGGGTGATTGGAATGGCGCTGGTGCTCACACCAACTACAGCACCAAATCAATGAGAAACGACGGTGGCATTGATGTGATCAAGAAAGCCATCGAGAAACTGGGATTGCGTCATAAACTGCACATCGCTGCCTATGGAGAAGGCAACGAGAGAAGGTTAACCGGCCGCCACGAGACTGCCGATATCAACACCTTCTCTTGGGGAGTTGCAAACAGAGGAGCCTCTGTCCGTGTCGGCCGCGACACTGAACAAGCCGGGAAAGGCAAGCTACTTCGAGGATCGAAGGCCAGCTTCAAACATGGATCCTTACGTCGTCACTTCCATGATCGCCGAGACAACCATTATGTGGAAGCCGTAGACGGACTCGATCAGCCGGCGTTCTGACGGCGCCGCCCCTATCCGGTTCATATCTGTTTCTATCTACTACATTTACTGCTTCTGAGAGTGATCTATGTCCAAAACTCTGGTTTCTGTATATTTGTGTGTGCCTCGTGATTGCGTTCGGCCATTGCTTCCTGTTGTTTCCCTTTTTCTTCTTTTATCGCAAACAATAATCAATTTATTTTCTTCTGCCCCATGCTTTTATCGTTCATGCCACTAAATGTCTCTTTTTATTTTTGGGAATTAAAAATGTATTCGTGTTATTATTGTCACTTCGCATGACATAAAGTAATTGTCAGCCTTCC

Coding sequence (CDS)

ATGTCCCTCCTCTCTGACCTCATCAATCTCAACCTCACTGATTCTACCGACAAGATCATTGCCGAATACGTATGGATTGGAGGATCGGGGCTCGATTTGAGAAGCAAAGCTAGGACTCTTCCGGGACTGGTGACGGATCCAGCGAAGCTCCCGAGGTGGAACTACGACGGTTCCAGTACGAATCAAGCCCCCGGAGACGACAGCGAAGTCATCTTATATCCCCAAGCTATTTTCAAGGATCCATTCAGAAGAGGAAACAACATCCTGGTTATGTGCGATGCTTATACGCCATCTGGCGAGCCGATTCCGACGAACAAGAGATTCAACGCCGCCAAGATTTTTAGCCACGCCGACGTCGTTGCGGAGGAACCTTGGTATGGGATTGAGCAGGAGTACACTCTCCTCCAAAAGGACGTCCACTGGCCACTCGGTTGGCCTGTAGGTGGCTTCCCAGGCCCACAGGGTCCTTACTATTGTGGCACAGGAGCAGACAAGGCCTTCGGTCGAGATATTGTAGACGCTCACTATAAGGCTTGTCTTTATGCCGGAGTCAATATCAGTGGAATCAACGGTGAAGTCATGCCCGGTCAGAGAATCACAGAAATTGCAGGAGTTGTTCTGTCCTTCGACCCAAAGCCAATTAAGGGTGATTGGAATGGCGCTGGTGCTCACACCAACTACAGCACCAAATCAATGAGAAACGACGGTGGCATTGATGTGATCAAGAAAGCCATCGAGAAACTGGGATTGCGTCATAAACTGCACATCGCTGCCTATGGAGAAGGCAACGAGAGAAGGTTAACCGGCCGCCACGAGACTGCCGATATCAACACCTTCTCTTGGGGAGTTGCAAACAGAGGAGCCTCTGTCCGTGTCGGCCGCGACACTGAACAAGCCGGGAAAGGCAAGCTACTTCGAGGATCGAAGGCCAGCTTCAAACATGGATCCTTACGTCGTCACTTCCATGATCGCCGAGACAACCATTATGTGGAAGCCGTAGACGGACTCGATCAGCCGGCGTTCTGA

Protein sequence

MSLLSDLINLNLTDSTDKIIAEYVWIGGSGLDLRSKARTLPGLVTDPAKLPRWNYDGSSTNQAPGDDSEVILYPQAIFKDPFRRGNNILVMCDAYTPSGEPIPTNKRFNAAKIFSHADVVAEEPWYGIEQEYTLLQKDVHWPLGWPVGGFPGPQGPYYCGTGADKAFGRDIVDAHYKACLYAGVNISGINGEVMPGQRITEIAGVVLSFDPKPIKGDWNGAGAHTNYSTKSMRNDGGIDVIKKAIEKLGLRHKLHIAAYGEGNERRLTGRHETADINTFSWGVANRGASVRVGRDTEQAGKGKLLRGSKASFKHGSLRRHFHDRRDNHYVEAVDGLDQPAF

Homology

[Close]

BLAST of CmaCh08G004990 vs. ExPASy Swiss-Prot
Match: P32289 (Glutamine synthetase nodule isozyme OS=Vigna aconitifolia OX=3918 PE=2 SV=1)

HSP 1 Score: 579.3 bits (1492), Expect = 2.8e-164
Identity = 276/327 (84.40%), Postives = 292/327 (89.30%), Query Frame = 0


Query: 1   MSLLSDLINLNLTDSTDKIIAEYVWIGGSGLDLRSKARTLPGLVTDPAKLPRWNYDGSST 60
           MSLLSDLINLNL+D+T+KIIAEY+WIGGSGLDLRSKARTLPG V+DP+KLP+WNYDGSST
Sbjct: 1   MSLLSDLINLNLSDTTEKIIAEYIWIGGSGLDLRSKARTLPGPVSDPSKLPKWNYDGSST 60

Query: 61  NQAPGDDSEVILYPQAIFKDPFRRGNNILVMCDAYTPSGEPIPTNKRFNAAKIFSHADVV 120
            QAPG+DSEVI+YPQAIFKDPFRRGNNILVMCDAYTP+GEPIPTNKR NAAKIFSH DVV
Sbjct: 61  GQAPGEDSEVIIYPQAIFKDPFRRGNNILVMCDAYTPAGEPIPTNKRHNAAKIFSHPDVV 120

Query: 121 AEEPWYGIEQEYTLLQKDVHWPLGWPVGGFPGPQGPYYCGTGADKAFGRDIVDAHYKACL 180
           AEEPWYGIEQEYTLLQKDV+WPLGWPVGGFPGPQGPYYCG GADKAFGRDIVDAHYKACL
Sbjct: 121 AEEPWYGIEQEYTLLQKDVNWPLGWPVGGFPGPQGPYYCGAGADKAFGRDIVDAHYKACL 180

Query: 181 YAGVNISGINGEVMPGQ-------------------------RITEIAGVVLSFDPKPIK 240
           YAG+NISGINGEVMPGQ                         RITEIAGVVLSFDPKPIK
Sbjct: 181 YAGINISGINGEVMPGQWEFQVGPAVGISAGDELWVARYILERITEIAGVVLSFDPKPIK 240

Query: 241 GDWNGAGAHTNYSTKSMRNDGGIDVIKKAIEKLGLRHKLHIAAYGEGNERRLTGRHETAD 300
           GDWNGAGAHTNYSTK+MRNDGG +VIK AIEKLG RHK HIAAYGEGNERRLTGRHETAD
Sbjct: 241 GDWNGAGAHTNYSTKTMRNDGGYEVIKSAIEKLGKRHKEHIAAYGEGNERRLTGRHETAD 300

Query: 301 INTFSWGVANRGASVRVGRDTEQAGKG 303
           INTF WGVANRGAS+RVGRDTE+AGKG
Sbjct: 301 INTFLWGVANRGASIRVGRDTEKAGKG 327

[Close]

BLAST of CmaCh08G004990 vs. ExPASy Swiss-Prot
Match: P04078 (Glutamine synthetase cytosolic isozyme OS=Medicago sativa OX=3879 PE=2 SV=1)

HSP 1 Score: 565.1 bits (1455), Expect = 5.5e-160
Identity = 268/327 (81.96%), Postives = 289/327 (88.38%), Query Frame = 0


Query: 1   MSLLSDLINLNLTDSTDKIIAEYVWIGGSGLDLRSKARTLPGLVTDPAKLPRWNYDGSST 60
           MSLLSDLINL+L+++T+KIIAEY+WIGGSGLDLRSKARTLPG VTDP++LP+WNYDGSST
Sbjct: 1   MSLLSDLINLDLSETTEKIIAEYIWIGGSGLDLRSKARTLPGPVTDPSQLPKWNYDGSST 60

Query: 61  NQAPGDDSEVILYPQAIFKDPFRRGNNILVMCDAYTPSGEPIPTNKRFNAAKIFSHADVV 120
            QAPG+DSEVI+YPQAIFKDPFRRGNNILVMCDAYTP+GEPIPTNKR  AAKIFSH DVV
Sbjct: 61  GQAPGEDSEVIIYPQAIFKDPFRRGNNILVMCDAYTPAGEPIPTNKRHAAAKIFSHPDVV 120

Query: 121 AEEPWYGIEQEYTLLQKDVHWPLGWPVGGFPGPQGPYYCGTGADKAFGRDIVDAHYKACL 180
           AE PWYGIEQEYTLLQKD++WPLGWPVGGFPGPQGPYYCG GADKAFGRDIVD+HYKACL
Sbjct: 121 AEVPWYGIEQEYTLLQKDINWPLGWPVGGFPGPQGPYYCGAGADKAFGRDIVDSHYKACL 180

Query: 181 YAGVNISGINGEVMPGQ-------------------------RITEIAGVVLSFDPKPIK 240
           YAG+NISGINGEVMPGQ                         RITE+AGVVLSFDPKPIK
Sbjct: 181 YAGINISGINGEVMPGQWEFQVGPSVGISAGDEIWVARYILERITEVAGVVLSFDPKPIK 240

Query: 241 GDWNGAGAHTNYSTKSMRNDGGIDVIKKAIEKLGLRHKLHIAAYGEGNERRLTGRHETAD 300
           GDWNGAGAHTNYSTKSMR DGG +VI KAIEKLG +HK HIAAYGEGNERRLTGRHETAD
Sbjct: 241 GDWNGAGAHTNYSTKSMREDGGYEVILKAIEKLGKKHKEHIAAYGEGNERRLTGRHETAD 300

Query: 301 INTFSWGVANRGASVRVGRDTEQAGKG 303
           INTF WGVANRGAS+RVGRDTE+AGKG
Sbjct: 301 INTFLWGVANRGASIRVGRDTEKAGKG 327

[Close]

BLAST of CmaCh08G004990 vs. ExPASy Swiss-Prot
Match: P51118 (Glutamine synthetase cytosolic isozyme 1 OS=Vitis vinifera OX=29760 GN=GS1-1 PE=2 SV=1)

HSP 1 Score: 565.1 bits (1455), Expect = 5.5e-160
Identity = 267/327 (81.65%), Postives = 288/327 (88.07%), Query Frame = 0


Query: 1   MSLLSDLINLNLTDSTDKIIAEYVWIGGSGLDLRSKARTLPGLVTDPAKLPRWNYDGSST 60
           M+LLSDLINLNL+++T+K+I EY+W+GGSG+DLRSKARTL G V+DPAKLP+WNYDGSST
Sbjct: 1   MALLSDLINLNLSETTEKVIVEYIWVGGSGMDLRSKARTLSGPVSDPAKLPKWNYDGSST 60

Query: 61  NQAPGDDSEVILYPQAIFKDPFRRGNNILVMCDAYTPSGEPIPTNKRFNAAKIFSHADVV 120
            QAPG+DSEVILYPQAIFKDPFRRGNNILVMCD YTP+GEPIPTNKR NAAKIFSH DV 
Sbjct: 61  GQAPGEDSEVILYPQAIFKDPFRRGNNILVMCDTYTPAGEPIPTNKRCNAAKIFSHPDVA 120

Query: 121 AEEPWYGIEQEYTLLQKDVHWPLGWPVGGFPGPQGPYYCGTGADKAFGRDIVDAHYKACL 180
           AE PWYGIEQEYTLLQK+V WP+GWPVGGFPGPQGPYYCG GADKA+GRDIVDAHYKACL
Sbjct: 121 AEVPWYGIEQEYTLLQKEVKWPIGWPVGGFPGPQGPYYCGIGADKAWGRDIVDAHYKACL 180

Query: 181 YAGVNISGINGEVMPGQ-------------------------RITEIAGVVLSFDPKPIK 240
           YAG+NISGINGEVMPGQ                         RITEIAGVVLSFDPKPI+
Sbjct: 181 YAGINISGINGEVMPGQWEYQVGPSVGISAGDELWVSRYILERITEIAGVVLSFDPKPIQ 240

Query: 241 GDWNGAGAHTNYSTKSMRNDGGIDVIKKAIEKLGLRHKLHIAAYGEGNERRLTGRHETAD 300
           GDWNGAGAHTNYSTKSMRNDGG +VIKKAIEKLGLRHK HIAAYGEGNERRLTGRHETAD
Sbjct: 241 GDWNGAGAHTNYSTKSMRNDGGFEVIKKAIEKLGLRHKEHIAAYGEGNERRLTGRHETAD 300

Query: 301 INTFSWGVANRGASVRVGRDTEQAGKG 303
           INTF WGVANRGAS+RVGRDTE+AGKG
Sbjct: 301 INTFLWGVANRGASIRVGRDTEKAGKG 327

[Close]

BLAST of CmaCh08G004990 vs. ExPASy Swiss-Prot
Match: Q43785 (Glutamine synthetase nodule isozyme OS=Medicago sativa OX=3879 GN=GS1 PE=2 SV=1)

HSP 1 Score: 563.5 bits (1451), Expect = 1.6e-159
Identity = 265/327 (81.04%), Postives = 287/327 (87.77%), Query Frame = 0


Query: 1   MSLLSDLINLNLTDSTDKIIAEYVWIGGSGLDLRSKARTLPGLVTDPAKLPRWNYDGSST 60
           MSLLSDLINLNL++S++KIIAEY+W+GGSG+DLRSKARTLPG V+DPAKLP+WNYDGSST
Sbjct: 1   MSLLSDLINLNLSESSEKIIAEYIWVGGSGMDLRSKARTLPGPVSDPAKLPKWNYDGSST 60

Query: 61  NQAPGDDSEVILYPQAIFKDPFRRGNNILVMCDAYTPSGEPIPTNKRFNAAKIFSHADVV 120
           NQAPG DSEVILYPQAIFKDPFR+GNNILV+CD YTP+GEP+PTNKR NAAKIFSH DV 
Sbjct: 61  NQAPGQDSEVILYPQAIFKDPFRQGNNILVICDVYTPAGEPLPTNKRHNAAKIFSHPDVA 120

Query: 121 AEEPWYGIEQEYTLLQKDVHWPLGWPVGGFPGPQGPYYCGTGADKAFGRDIVDAHYKACL 180
           AE PWYGIEQEYTLLQKD +WPLGWP+GGFPGPQGPYYCG GADKA+GRDIVDAHYKACL
Sbjct: 121 AEVPWYGIEQEYTLLQKDTNWPLGWPIGGFPGPQGPYYCGIGADKAYGRDIVDAHYKACL 180

Query: 181 YAGVNISGINGEVMPGQ-------------------------RITEIAGVVLSFDPKPIK 240
           YAG+NISGINGEVMPGQ                         RITEIAGVV+SFDPKPI 
Sbjct: 181 YAGINISGINGEVMPGQWEFQVGPSVGISAGDEIWAARYILERITEIAGVVVSFDPKPIP 240

Query: 241 GDWNGAGAHTNYSTKSMRNDGGIDVIKKAIEKLGLRHKLHIAAYGEGNERRLTGRHETAD 300
           GDWNGAGAHTNYSTKSMR DGG ++IKKAIEKLGLRHK HIAAYGEGNERRLTG+HET D
Sbjct: 241 GDWNGAGAHTNYSTKSMREDGGYEIIKKAIEKLGLRHKEHIAAYGEGNERRLTGKHETTD 300

Query: 301 INTFSWGVANRGASVRVGRDTEQAGKG 303
           INTFSWGVANRGASVRVGRDTE+ GKG
Sbjct: 301 INTFSWGVANRGASVRVGRDTEKDGKG 327

[Close]

BLAST of CmaCh08G004990 vs. ExPASy Swiss-Prot
Match: Q42899 (Glutamine synthetase cytosolic isozyme OS=Lotus japonicus OX=34305 GN=GLN1 PE=2 SV=2)

HSP 1 Score: 562.8 bits (1449), Expect = 2.7e-159
Identity = 266/327 (81.35%), Postives = 290/327 (88.69%), Query Frame = 0


Query: 1   MSLLSDLINLNLTDSTDKIIAEYVWIGGSGLDLRSKARTLPGLVTDPAKLPRWNYDGSST 60
           MSLLSDLINLNL+++TDKIIAEY+WIGGSGLD+RSKARTLPG V+DP++LP+WNYDGSST
Sbjct: 1   MSLLSDLINLNLSETTDKIIAEYIWIGGSGLDMRSKARTLPGPVSDPSQLPKWNYDGSST 60

Query: 61  NQAPGDDSEVILYPQAIFKDPFRRGNNILVMCDAYTPSGEPIPTNKRFNAAKIFSHADVV 120
            QAPG+DSEVILYPQAIF+DPFRRG+NILV+CDAYTP+GEPIPTNKR  AAK+FSH DVV
Sbjct: 61  GQAPGEDSEVILYPQAIFRDPFRRGSNILVICDAYTPAGEPIPTNKRHAAAKVFSHPDVV 120

Query: 121 AEEPWYGIEQEYTLLQKDVHWPLGWPVGGFPGPQGPYYCGTGADKAFGRDIVDAHYKACL 180
           AE PWYGIEQEYTLLQK+V+WP+GWP+GGFPGPQGPYYCG GADKAFGRDIVDAHYKACL
Sbjct: 121 AEVPWYGIEQEYTLLQKEVNWPVGWPIGGFPGPQGPYYCGIGADKAFGRDIVDAHYKACL 180

Query: 181 YAGVNISGINGEVMPGQ-------------------------RITEIAGVVLSFDPKPIK 240
           YAGVNISGINGEVMPGQ                         RITEIAGVVLSFDPKPIK
Sbjct: 181 YAGVNISGINGEVMPGQWEFQVGPSVGISAGDEVWVARYILERITEIAGVVLSFDPKPIK 240

Query: 241 GDWNGAGAHTNYSTKSMRNDGGIDVIKKAIEKLGLRHKLHIAAYGEGNERRLTGRHETAD 300
           GDWNGAGAHTNYSTK+MR DGG +VIKKAI+KLGLRHK HIAAYGEGNERRLTGRHETAD
Sbjct: 241 GDWNGAGAHTNYSTKTMREDGGYEVIKKAIDKLGLRHKEHIAAYGEGNERRLTGRHETAD 300

Query: 301 INTFSWGVANRGASVRVGRDTEQAGKG 303
           INTF WGVANRGAS+RVGRDTE+ GKG
Sbjct: 301 INTFLWGVANRGASIRVGRDTEKEGKG 327

[Close]

BLAST of CmaCh08G004990 vs. TAIR 10
Match: AT5G16570.1 (glutamine synthetase 1;4 )

HSP 1 Score: 554.7 bits (1428), Expect = 5.3e-158
Identity = 263/327 (80.43%), Postives = 283/327 (86.54%), Query Frame = 0


Query: 1   MSLLSDLINLNLTDSTDKIIAEYVWIGGSGLDLRSKARTLPGLVTDPAKLPRWNYDGSST 60
           MS L+DLINL+L+DSTD+IIAEY+WIGGSGLD+RSKARTLPG VTDP++LP+WNYDGSST
Sbjct: 1   MSSLADLINLDLSDSTDQIIAEYIWIGGSGLDMRSKARTLPGPVTDPSQLPKWNYDGSST 60

Query: 61  NQAPGDDSEVILYPQAIFKDPFRRGNNILVMCDAYTPSGEPIPTNKRFNAAKIFSHADVV 120
            QAPGDDSEVI+YPQAIFKDPFRRGNNILVMCDAYTP+GEPIPTNKR  AAKIF    VV
Sbjct: 61  GQAPGDDSEVIIYPQAIFKDPFRRGNNILVMCDAYTPAGEPIPTNKRHAAAKIFEDPSVV 120

Query: 121 AEEPWYGIEQEYTLLQKDVHWPLGWPVGGFPGPQGPYYCGTGADKAFGRDIVDAHYKACL 180
           AEE WYGIEQEYTLLQKD+ WP+GWPVGGFPGPQGPYYCG GADKAFGRDIVD+HYKACL
Sbjct: 121 AEETWYGIEQEYTLLQKDIKWPVGWPVGGFPGPQGPYYCGVGADKAFGRDIVDSHYKACL 180

Query: 181 YAGVNISGINGEVMPGQ-------------------------RITEIAGVVLSFDPKPIK 240
           YAG+N+SG NGEVMPGQ                         RITE+AGVVLS DPKPI 
Sbjct: 181 YAGINVSGTNGEVMPGQWEFQVGPTVGIAAADQVWVARYILERITELAGVVLSLDPKPIP 240

Query: 241 GDWNGAGAHTNYSTKSMRNDGGIDVIKKAIEKLGLRHKLHIAAYGEGNERRLTGRHETAD 300
           GDWNGAGAHTNYSTKSMR DGG +VIKKAIEKLGLRHK HIAAYGEGNERRLTG+HETAD
Sbjct: 241 GDWNGAGAHTNYSTKSMREDGGYEVIKKAIEKLGLRHKEHIAAYGEGNERRLTGKHETAD 300

Query: 301 INTFSWGVANRGASVRVGRDTEQAGKG 303
           INTF WGVANRGAS+RVGRDTEQAGKG
Sbjct: 301 INTFLWGVANRGASIRVGRDTEQAGKG 327

[Close]

BLAST of CmaCh08G004990 vs. TAIR 10
Match: AT3G17820.1 (glutamine synthetase 1.3 )

HSP 1 Score: 554.3 bits (1427), Expect = 6.9e-158
Identity = 259/327 (79.20%), Postives = 285/327 (87.16%), Query Frame = 0


Query: 1   MSLLSDLINLNLTDSTDKIIAEYVWIGGSGLDLRSKARTLPGLVTDPAKLPRWNYDGSST 60
           MSLLSDL+NLNLTD+T KIIAEY+WIGGSG+D+RSKARTLPG VTDP+KLP+WNYDGSST
Sbjct: 1   MSLLSDLVNLNLTDATGKIIAEYIWIGGSGMDIRSKARTLPGPVTDPSKLPKWNYDGSST 60

Query: 61  NQAPGDDSEVILYPQAIFKDPFRRGNNILVMCDAYTPSGEPIPTNKRFNAAKIFSHADVV 120
            QA G+DSEVILYPQAIFKDPFR+GNNILVMCDAYTP+G+PIPTNKR NAAKIFSH DV 
Sbjct: 61  GQAAGEDSEVILYPQAIFKDPFRKGNNILVMCDAYTPAGDPIPTNKRHNAAKIFSHPDVA 120

Query: 121 AEEPWYGIEQEYTLLQKDVHWPLGWPVGGFPGPQGPYYCGTGADKAFGRDIVDAHYKACL 180
            EEPWYGIEQEYTL+QKDV+WP+GWPVGG+PGPQGPYYCG GADKA GRDIVDAHYKACL
Sbjct: 121 KEEPWYGIEQEYTLMQKDVNWPIGWPVGGYPGPQGPYYCGVGADKAIGRDIVDAHYKACL 180

Query: 181 YAGVNISGINGEVMPGQ-------------------------RITEIAGVVLSFDPKPIK 240
           YAG+ ISGINGEVMPGQ                         RITEI+GV++SFDPKP+ 
Sbjct: 181 YAGIGISGINGEVMPGQWEFQVGPVEGISSGDQVWVARYLLERITEISGVIVSFDPKPVP 240

Query: 241 GDWNGAGAHTNYSTKSMRNDGGIDVIKKAIEKLGLRHKLHIAAYGEGNERRLTGRHETAD 300
           GDWNGAGAH NYSTK+MRNDGG++VIKKAI KL L+HK HIAAYGEGNERRLTG+HETAD
Sbjct: 241 GDWNGAGAHCNYSTKTMRNDGGLEVIKKAIGKLQLKHKEHIAAYGEGNERRLTGKHETAD 300

Query: 301 INTFSWGVANRGASVRVGRDTEQAGKG 303
           INTFSWGVANRGASVRVGRDTE+ GKG
Sbjct: 301 INTFSWGVANRGASVRVGRDTEKEGKG 327

[Close]

BLAST of CmaCh08G004990 vs. TAIR 10
Match: AT5G37600.1 (glutamine synthase clone R1 )

HSP 1 Score: 553.1 bits (1424), Expect = 1.5e-157
Identity = 258/327 (78.90%), Postives = 285/327 (87.16%), Query Frame = 0


Query: 1   MSLLSDLINLNLTDSTDKIIAEYVWIGGSGLDLRSKARTLPGLVTDPAKLPRWNYDGSST 60
           MSL+SDLINLNL+DSTDKIIAEY+W+GGSG+D+RSKARTLPG VTDP++LP+WNYDGSST
Sbjct: 1   MSLVSDLINLNLSDSTDKIIAEYIWVGGSGMDMRSKARTLPGPVTDPSQLPKWNYDGSST 60

Query: 61  NQAPGDDSEVILYPQAIFKDPFRRGNNILVMCDAYTPSGEPIPTNKRFNAAKIFSHADVV 120
            QAPG+DSEVILYPQAIFKDPFRRGNNILVMCDAYTP+GEPIPTNKR  AAK+FS+ DV 
Sbjct: 61  GQAPGEDSEVILYPQAIFKDPFRRGNNILVMCDAYTPAGEPIPTNKRHAAAKVFSNPDVA 120

Query: 121 AEEPWYGIEQEYTLLQKDVHWPLGWPVGGFPGPQGPYYCGTGADKAFGRDIVDAHYKACL 180
           AE PWYGIEQEYTLLQKDV WP+GWP+GG+PGPQGPYYCG GADK+FGRD+VD+HYKACL
Sbjct: 121 AEVPWYGIEQEYTLLQKDVKWPVGWPIGGYPGPQGPYYCGIGADKSFGRDVVDSHYKACL 180

Query: 181 YAGVNISGINGEVMPGQ-------------------------RITEIAGVVLSFDPKPIK 240
           YAG+NISGINGEVMPGQ                         RITEIAGVV+SFDPKPI 
Sbjct: 181 YAGINISGINGEVMPGQWEFQVGPAVGISAADEIWVARYILERITEIAGVVVSFDPKPIP 240

Query: 241 GDWNGAGAHTNYSTKSMRNDGGIDVIKKAIEKLGLRHKLHIAAYGEGNERRLTGRHETAD 300
           GDWNGAGAH NYSTKSMR +GG ++IKKAI+KLGLRHK HIAAYGEGNERRLTG HETAD
Sbjct: 241 GDWNGAGAHCNYSTKSMREEGGYEIIKKAIDKLGLRHKEHIAAYGEGNERRLTGHHETAD 300

Query: 301 INTFSWGVANRGASVRVGRDTEQAGKG 303
           INTF WGVANRGAS+RVGRDTE+ GKG
Sbjct: 301 INTFLWGVANRGASIRVGRDTEKEGKG 327

[Close]

BLAST of CmaCh08G004990 vs. TAIR 10
Match: AT1G66200.1 (glutamine synthase clone F11 )

HSP 1 Score: 546.2 bits (1406), Expect = 1.9e-155
Identity = 255/327 (77.98%), Postives = 285/327 (87.16%), Query Frame = 0


Query: 1   MSLLSDLINLNLTDSTDKIIAEYVWIGGSGLDLRSKARTLPGLVTDPAKLPRWNYDGSST 60
           MSLL+DL+NL+++D+++KIIAEY+W+GGSG+D+RSKARTLPG VTDP+KLP+WNYDGSST
Sbjct: 1   MSLLADLVNLDISDNSEKIIAEYIWVGGSGMDMRSKARTLPGPVTDPSKLPKWNYDGSST 60

Query: 61  NQAPGDDSEVILYPQAIFKDPFRRGNNILVMCDAYTPSGEPIPTNKRFNAAKIFSHADVV 120
            QAPG DSEVILYPQAIFKDPFRRGNNILVMCDAYTP+GEPIPTNKR  AA+IF++ DV+
Sbjct: 61  GQAPGQDSEVILYPQAIFKDPFRRGNNILVMCDAYTPAGEPIPTNKRHAAAEIFANPDVI 120

Query: 121 AEEPWYGIEQEYTLLQKDVHWPLGWPVGGFPGPQGPYYCGTGADKAFGRDIVDAHYKACL 180
           AE PWYGIEQEYTLLQKDV+WPLGWP+GGFPGPQGPYYC  GADK+FGRDIVDAHYKA L
Sbjct: 121 AEVPWYGIEQEYTLLQKDVNWPLGWPIGGFPGPQGPYYCSIGADKSFGRDIVDAHYKASL 180

Query: 181 YAGVNISGINGEVMPGQ-------------------------RITEIAGVVLSFDPKPIK 240
           YAG+NISGINGEVMPGQ                         RITEIAGVV+SFDPKPI 
Sbjct: 181 YAGINISGINGEVMPGQWEFQVGPSVGISAADEIWIARYILERITEIAGVVVSFDPKPIP 240

Query: 241 GDWNGAGAHTNYSTKSMRNDGGIDVIKKAIEKLGLRHKLHIAAYGEGNERRLTGRHETAD 300
           GDWNGAGAHTNYSTKSMR +GG ++IKKAIEKLGLRHK HI+AYGEGNERRLTG HETAD
Sbjct: 241 GDWNGAGAHTNYSTKSMREEGGYEIIKKAIEKLGLRHKEHISAYGEGNERRLTGHHETAD 300

Query: 301 INTFSWGVANRGASVRVGRDTEQAGKG 303
           INTF WGVANRGAS+RVGRDTE+ GKG
Sbjct: 301 INTFLWGVANRGASIRVGRDTEKEGKG 327

[Close]

BLAST of CmaCh08G004990 vs. TAIR 10
Match: AT1G66200.3 (glutamine synthase clone F11 )

HSP 1 Score: 538.5 bits (1386), Expect = 3.9e-153
Identity = 255/336 (75.89%), Postives = 285/336 (84.82%), Query Frame = 0


Query: 1   MSLLSDLINLNLTDSTDKIIAEYVWIGGSGLDLRSKARTLPGLVTDPAKLPRWNYDGSST 60
           MSLL+DL+NL+++D+++KIIAEY+W+GGSG+D+RSKARTLPG VTDP+KLP+WNYDGSST
Sbjct: 1   MSLLADLVNLDISDNSEKIIAEYIWVGGSGMDMRSKARTLPGPVTDPSKLPKWNYDGSST 60

Query: 61  NQAPGDDSEVILYPQAIFKDPFRRGNNIL---------VMCDAYTPSGEPIPTNKRFNAA 120
            QAPG DSEVILYPQAIFKDPFRRGNNIL         VMCDAYTP+GEPIPTNKR  AA
Sbjct: 61  GQAPGQDSEVILYPQAIFKDPFRRGNNILINVFYRFDQVMCDAYTPAGEPIPTNKRHAAA 120

Query: 121 KIFSHADVVAEEPWYGIEQEYTLLQKDVHWPLGWPVGGFPGPQGPYYCGTGADKAFGRDI 180
           +IF++ DV+AE PWYGIEQEYTLLQKDV+WPLGWP+GGFPGPQGPYYC  GADK+FGRDI
Sbjct: 121 EIFANPDVIAEVPWYGIEQEYTLLQKDVNWPLGWPIGGFPGPQGPYYCSIGADKSFGRDI 180

Query: 181 VDAHYKACLYAGVNISGINGEVMPGQ-------------------------RITEIAGVV 240
           VDAHYKA LYAG+NISGINGEVMPGQ                         RITEIAGVV
Sbjct: 181 VDAHYKASLYAGINISGINGEVMPGQWEFQVGPSVGISAADEIWIARYILERITEIAGVV 240

Query: 241 LSFDPKPIKGDWNGAGAHTNYSTKSMRNDGGIDVIKKAIEKLGLRHKLHIAAYGEGNERR 300
           +SFDPKPI GDWNGAGAHTNYSTKSMR +GG ++IKKAIEKLGLRHK HI+AYGEGNERR
Sbjct: 241 VSFDPKPIPGDWNGAGAHTNYSTKSMREEGGYEIIKKAIEKLGLRHKEHISAYGEGNERR 300

Query: 301 LTGRHETADINTFSWGVANRGASVRVGRDTEQAGKG 303
           LTG HETADINTF WGVANRGAS+RVGRDTE+ GKG
Sbjct: 301 LTGHHETADINTFLWGVANRGASIRVGRDTEKEGKG 336

The following BLAST results are available for this feature:

BLAST of CmaCh08G004990 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P32289	2.8e-164	84.40	Glutamine synthetase nodule isozyme OS=Vigna aconitifolia OX=3918 PE=2 SV=1	[more]
P04078	5.5e-160	81.96	Glutamine synthetase cytosolic isozyme OS=Medicago sativa OX=3879 PE=2 SV=1	[more]
P51118	5.5e-160	81.65	Glutamine synthetase cytosolic isozyme 1 OS=Vitis vinifera OX=29760 GN=GS1-1 PE=...	[more]
Q43785	1.6e-159	81.04	Glutamine synthetase nodule isozyme OS=Medicago sativa OX=3879 GN=GS1 PE=2 SV=1	[more]
Q42899	2.7e-159	81.35	Glutamine synthetase cytosolic isozyme OS=Lotus japonicus OX=34305 GN=GLN1 PE=2 ...	[more]

BLAST of CmaCh08G004990 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G16570.1	5.3e-158	80.43	glutamine synthetase 1;4	[more]
AT3G17820.1	6.9e-158	79.20	glutamine synthetase 1.3	[more]
AT5G37600.1	1.5e-157	78.90	glutamine synthase clone R1	[more]
AT1G66200.1	1.9e-155	77.98	glutamine synthase clone F11	[more]
AT1G66200.3	3.9e-153	75.89	glutamine synthase clone F11	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR008146	Glutamine synthetase, catalytic domain	SMART	SM01230	Gln_synt_C_2	coord: 103..333 e-value: 3.5E-17 score: 73.1
None	No IPR available	GENE3D	3.30.590.10	Glutamine synthetase/guanido kinase, catalytic domain	coord: 104..197 e-value: 1.6E-40 score: 140.8
None	No IPR available	GENE3D	3.30.590.10	Glutamine synthetase/guanido kinase, catalytic domain	coord: 198..305 e-value: 1.1E-33 score: 118.9
None	No IPR available	PANTHER	PTHR20852	GLUTAMINE SYNTHETASE	coord: 198..304
None	No IPR available	PANTHER	PTHR20852:SF89	GLUTAMINE SYNTHETASE	coord: 1..197
None	No IPR available	PANTHER	PTHR20852	GLUTAMINE SYNTHETASE	coord: 1..197
None	No IPR available	PANTHER	PTHR20852:SF89	GLUTAMINE SYNTHETASE	coord: 198..304
IPR008147	Glutamine synthetase, beta-Grasp domain	PFAM	PF03951	Gln-synt_N	coord: 48..97 e-value: 2.8E-9 score: 36.5
IPR036651	Glutamine synthetase, N-terminal domain superfamily	GENE3D	3.10.20.70		coord: 1..103 e-value: 7.1E-44 score: 149.9
IPR036651	Glutamine synthetase, N-terminal domain superfamily	SUPERFAMILY	54368	Glutamine synthetase, N-terminal domain	coord: 12..102
IPR027303	Glutamine synthetase, glycine-rich site	PROSITE	PS00181	GLNA_ATP	coord: 212..228
IPR027302	Glutamine synthetase, N-terminal conserved site	PROSITE	PS00180	GLNA_1	coord: 55..72
IPR014746	Glutamine synthetase/guanido kinase, catalytic domain	SUPERFAMILY	55931	Glutamine synthetase/guanido kinase	coord: 103..300

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmaCh08G004990.1	CmaCh08G004990.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006542	glutamine biosynthetic process
biological_process	GO:0006807	nitrogen compound metabolic process
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0004356	glutamate-ammonia ligase activity
molecular_function	GO:0003824	catalytic activity