CmaCh08G004850 (gene) Cucurbita maxima (Rimu) v1.1

Overview


Name	CmaCh08G004850
Type	gene
Organism	Cucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Description	gibberellin 2-beta-dioxygenase 2
Location	Cma_Chr08: 2762847 .. 2764115 (+)
RNA-Seq Expression	CmaCh08G004850
Synteny	CmaCh08G004850

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCCTTTCCCGACAGCCACGAGGTCCAAGAAGACAAAGGCCGTCGGAATTCCGACCATAGATCTCTCCGAAAGCAGAGCAAGAACTTCCAAATCCATTGTCGAGGCCTGCCAGCACTTTGGCTTCTTCAAAGTCATCAACCATGGAGTTTCTGACCATGCCATTGCGAATCTTGAACGCCAAGCCTTTGATTTCTTTGGCCAAACGGCGCCTGAGAAGCGTCGTGCTGGCCCTGCCGCCCCCTTCGGCTATGGCTGCAAAGCTATCGGCCCCAACGGCGACATCGGCGACCTCGAGTATATCCTTCTTCACTCCGACCCTGCTTCCATTGCTGAAACCTCCGCCTCCATCTCTAACAGTCCTCTTGCTTTTAGGTACTGCTTAGCTAGAAGGAAAGAAATATAATTTTTAACTGAAACATTGATTTATGTCGACATGTCGTCTGATATCTTAATTTGATGCAGCTATGTTGTGAATGAGTACGTTAAAAGTGTAAGAGAATTGGCATGTGAGATTCTTGATCTGGTGGGGGAGGGTCTATGGTTACCAAACAAGTCTGTCTTCAGTAACCTCATCAGAGACGTCCAAAGTGATTCCGTTTTGAGGATCAATTACTACCCTGCAGCAGTCAAGCACACTGAAAAAAGTAGGGAACTTTCATCAAAATCCCACCTTTCAACTAACAACATTGGATTTGGGGAGCATTCTGACCCTCAGATCTTGACCATCTTGAGATCGAACGATGTCGATGGCCTCCAAATCTCCTTGCACGACGGGTTGTGGACACCTGTTTGCCCCGACCCGTCTGCATTCTACATCTTGATTGGTGATGCGTTTCAGGTAAATTCTATGTCAATAGATCAAAGTATGAAAACTATTATATGAGATGCACGTAACAAGTCTAGTGTCAAACGAGACATCGATTTTTTACTTAAAACCTAAAGAACGAAACATGATCACGAACATTAGAAGCAAAAACATTTACAAAATGGGTTATTGAGACTTGTTCTTGTTTTTGATCAGGCAATGACAAATGATAGATTTGTAAGTGTGAGGCATAGGGCAATGGCAAACAACTCAAAGAAGGCAAGAATGTCAATCATGTACTTTGGGGCACCACCATTGAATGCATGGATAGCTCCTCCATCAGATATGATGCTCCCCAAGGACCAATCTCTATATAAGCCTTTCACTTGGGCTCAATACAAGAAAGCTGCTTATTCTCTTAGATTAGCTGACTCTCGTCTTCATCTCTTCAAAGCTTCTTAA

mRNA sequence

ATGCCTTTCCCGACAGCCACGAGGTCCAAGAAGACAAAGGCCGTCGGAATTCCGACCATAGATCTCTCCGAAAGCAGAGCAAGAACTTCCAAATCCATTGTCGAGGCCTGCCAGCACTTTGGCTTCTTCAAAGTCATCAACCATGGAGTTTCTGACCATGCCATTGCGAATCTTGAACGCCAAGCCTTTGATTTCTTTGGCCAAACGGCGCCTGAGAAGCGTCGTGCTGGCCCTGCCGCCCCCTTCGGCTATGGCTGCAAAGCTATCGGCCCCAACGGCGACATCGGCGACCTCGAGTATATCCTTCTTCACTCCGACCCTGCTTCCATTGCTGAAACCTCCGCCTCCATCTCTAACAGTCCTCTTGCTTTTAGCTATGTTGTGAATGAGTACGTTAAAAGTGTAAGAGAATTGGCATGTGAGATTCTTGATCTGGTGGGGGAGGGTCTATGGTTACCAAACAAGTCTGTCTTCAGTAACCTCATCAGAGACGTCCAAAGTGATTCCGTTTTGAGGATCAATTACTACCCTGCAGCAGTCAAGCACACTGAAAAAAGTAGGGAACTTTCATCAAAATCCCACCTTTCAACTAACAACATTGGATTTGGGGAGCATTCTGACCCTCAGATCTTGACCATCTTGAGATCGAACGATGTCGATGGCCTCCAAATCTCCTTGCACGACGGGTTGTGGACACCTGTTTGCCCCGACCCGTCTGCATTCTACATCTTGATTGGTGATGCGTTTCAGGCAATGACAAATGATAGATTTGTAAGTGTGAGGCATAGGGCAATGGCAAACAACTCAAAGAAGGCAAGAATGTCAATCATGTACTTTGGGGCACCACCATTGAATGCATGGATAGCTCCTCCATCAGATATGATGCTCCCCAAGGACCAATCTCTATATAAGCCTTTCACTTGGGCTCAATACAAGAAAGCTGCTTATTCTCTTAGATTAGCTGACTCTCGTCTTCATCTCTTCAAAGCTTCTTAA

Coding sequence (CDS)

ATGCCTTTCCCGACAGCCACGAGGTCCAAGAAGACAAAGGCCGTCGGAATTCCGACCATAGATCTCTCCGAAAGCAGAGCAAGAACTTCCAAATCCATTGTCGAGGCCTGCCAGCACTTTGGCTTCTTCAAAGTCATCAACCATGGAGTTTCTGACCATGCCATTGCGAATCTTGAACGCCAAGCCTTTGATTTCTTTGGCCAAACGGCGCCTGAGAAGCGTCGTGCTGGCCCTGCCGCCCCCTTCGGCTATGGCTGCAAAGCTATCGGCCCCAACGGCGACATCGGCGACCTCGAGTATATCCTTCTTCACTCCGACCCTGCTTCCATTGCTGAAACCTCCGCCTCCATCTCTAACAGTCCTCTTGCTTTTAGCTATGTTGTGAATGAGTACGTTAAAAGTGTAAGAGAATTGGCATGTGAGATTCTTGATCTGGTGGGGGAGGGTCTATGGTTACCAAACAAGTCTGTCTTCAGTAACCTCATCAGAGACGTCCAAAGTGATTCCGTTTTGAGGATCAATTACTACCCTGCAGCAGTCAAGCACACTGAAAAAAGTAGGGAACTTTCATCAAAATCCCACCTTTCAACTAACAACATTGGATTTGGGGAGCATTCTGACCCTCAGATCTTGACCATCTTGAGATCGAACGATGTCGATGGCCTCCAAATCTCCTTGCACGACGGGTTGTGGACACCTGTTTGCCCCGACCCGTCTGCATTCTACATCTTGATTGGTGATGCGTTTCAGGCAATGACAAATGATAGATTTGTAAGTGTGAGGCATAGGGCAATGGCAAACAACTCAAAGAAGGCAAGAATGTCAATCATGTACTTTGGGGCACCACCATTGAATGCATGGATAGCTCCTCCATCAGATATGATGCTCCCCAAGGACCAATCTCTATATAAGCCTTTCACTTGGGCTCAATACAAGAAAGCTGCTTATTCTCTTAGATTAGCTGACTCTCGTCTTCATCTCTTCAAAGCTTCTTAA

Protein sequence

MPFPTATRSKKTKAVGIPTIDLSESRARTSKSIVEACQHFGFFKVINHGVSDHAIANLERQAFDFFGQTAPEKRRAGPAAPFGYGCKAIGPNGDIGDLEYILLHSDPASIAETSASISNSPLAFSYVVNEYVKSVRELACEILDLVGEGLWLPNKSVFSNLIRDVQSDSVLRINYYPAAVKHTEKSRELSSKSHLSTNNIGFGEHSDPQILTILRSNDVDGLQISLHDGLWTPVCPDPSAFYILIGDAFQAMTNDRFVSVRHRAMANNSKKARMSIMYFGAPPLNAWIAPPSDMMLPKDQSLYKPFTWAQYKKAAYSLRLADSRLHLFKAS

Homology

[Close]

BLAST of CmaCh08G004850 vs. ExPASy Swiss-Prot
Match: Q9XHM5 (Gibberellin 2-beta-dioxygenase 2 OS=Pisum sativum OX=3888 GN=GA2OX2 PE=2 SV=1)

HSP 1 Score: 393.7 bits (1010), Expect = 2.1e-108
Identity = 192/331 (58.01%), Postives = 250/331 (75.53%), Query Frame = 0


Query: 1   MPFPTA-TRSKKTKAVGIPTIDLSESRARTSKSIVEACQHFGFFKVINHGVSDHAIANLE 60
           +P PT+  R+KKTKAVGIPTIDLS  R++ S+ +V+AC+ +GFFKV+NH V    I+ L+
Sbjct: 3   VPSPTSMIRTKKTKAVGIPTIDLSLERSQLSELVVKACEEYGFFKVVNHSVPKEVISRLD 62

Query: 61  RQAFDFFGQTAPEKRRAGPAAPFGYGCKAIGPNGDIGDLEYILLHSDPASIAETSASIS- 120
            +  +FF + + EKR+AG + PFGYGCK IGPNGD G+LEY+LLHS+P SI+E S +I+ 
Sbjct: 63  EEGIEFFSKNSSEKRQAGTSTPFGYGCKNIGPNGDKGELEYLLLHSNPISISERSKTIAK 122

Query: 121 NSPLAFSYVVNEYVKSVRELACEILDLVGEGLWLPNKSVFSNLIRDVQSDSVLRINYYPA 180
           + P+ FS +VN+Y+K+V++L CEIL+L  EGLW+P+KS  S +I+D  SDS+LRIN+YP 
Sbjct: 123 DHPIKFSCIVNDYIKAVKDLTCEILELAAEGLWVPDKSSLSKIIKDEHSDSLLRINHYPP 182

Query: 181 AVKHTEKSRELSSKSHLSTNNIGFGEHSDPQILTILRSNDVDGLQISLHDGLWTPVCPDP 240
             K    + + S   + + NNIGFGEHSDPQILTILRSN+V GLQIS H GLW PV PDP
Sbjct: 183 VKKLGNDNWDPSKIQNSNNNNIGFGEHSDPQILTILRSNNVGGLQISTHHGLWIPVPPDP 242

Query: 241 SAFYILIGDAFQAMTNDRFVSVRHRAMANNSKKARMSIMYFGAPPLNAWIAPPSDMMLPK 300
           S FY+++GDA Q +TN RFVSVRHR + N + K RMS+MYF APPLN  I+P S M+   
Sbjct: 243 SEFYVMVGDALQVLTNGRFVSVRHRVLTNTT-KPRMSMMYFAAPPLNWLISPLSKMVTAH 302

Query: 301 DQSLYKPFTWAQYKKAAYSLRLADSRLHLFK 330
              LY+PFTWAQYK+AAY+LRL D+RL  FK
Sbjct: 303 SPCLYRPFTWAQYKQAAYALRLGDTRLDQFK 332

[Close]

BLAST of CmaCh08G004850 vs. ExPASy Swiss-Prot
Match: Q9FZ21 (Gibberellin 2-beta-dioxygenase 6 OS=Arabidopsis thaliana OX=3702 GN=GA2OX6 PE=2 SV=1)

HSP 1 Score: 337.4 bits (864), Expect = 1.8e-91
Identity = 173/331 (52.27%), Postives = 218/331 (65.86%), Query Frame = 0


Query: 4   PTATRSKKT---KAVGIPTIDLS-ESRARTSKSIVEACQHFGFFKVINHGVSDHAIANLE 63
           P  T  KKT        P ID S   R++ S+ IV+AC+  GFFKVINHGV    I   E
Sbjct: 8   PLQTTGKKTISSPEYNFPVIDFSLNDRSKLSEKIVKACEVNGFFKVINHGVKPEIIKRFE 67

Query: 64  RQAFDFFGQTAPEKRRAGPAAPFGYGCKAIGPNGDIGDLEYILLHSDPASIAETSASIS- 123
            +  +FF +   +K RAGPA+PFGYGCK IG NGD+G+LEY+LLH++P ++A+ S +IS 
Sbjct: 68  HEGEEFFNKPESDKLRAGPASPFGYGCKNIGFNGDLGELEYLLLHANPTAVADKSETISH 127

Query: 124 NSPLAFSYVVNEYVKSVRELACEILDLVGEGLWLPNKSVFSNLIRDVQSDSVLRINYYPA 183
           + P  FS   N+Y+++VR+LACEI+DL  E LW    S  S LIRDV+SDS+LR+N+YP 
Sbjct: 128 DDPFKFSSATNDYIRTVRDLACEIIDLTIENLWGQKSSEVSELIRDVRSDSILRLNHYPP 187

Query: 184 AVKHTEKSRELSSKSHLSTNNIGFGEHSDPQILTILRSNDVDGLQISLHDGLWTPVCPDP 243
           A             +      IGFGEHSDPQILT+LRSNDVDGL+I   DGLW P+  DP
Sbjct: 188 A-----------PYALSGVGQIGFGEHSDPQILTVLRSNDVDGLEICSRDGLWIPIPSDP 247

Query: 244 SAFYILIGDAFQAMTNDRFVSVRHRAMANNSKKARMSIMYFGAPPLNAWIAPPSDMMLPK 303
           + F++L+GD  QA+TN RF SVRHR +AN +KK RMS MYF APPL A I+P   M+ P+
Sbjct: 248 TCFFVLVGDCLQALTNGRFTSVRHRVLANTAKKPRMSAMYFAAPPLEAKISPLPKMVSPE 307

Query: 304 DQSLYKPFTWAQYKKAAYSLRLADSRLHLFK 330
           +   Y  FTW  YKKA YSLRL   RL  FK
Sbjct: 308 NPRRYNSFTWGDYKKATYSLRLDVPRLEFFK 327

[Close]

BLAST of CmaCh08G004850 vs. ExPASy Swiss-Prot
Match: Q9C7Z1 (Gibberellin 2-beta-dioxygenase 4 OS=Arabidopsis thaliana OX=3702 GN=GA2OX4 PE=2 SV=1)

HSP 1 Score: 292.7 bits (748), Expect = 5.1e-78
Identity = 164/326 (50.31%), Postives = 219/326 (67.18%), Query Frame = 0


Query: 9   SKKTKAV--GIPTIDLSESRARTSKSIVEACQHFGFFKVINHGVSDHAIANLERQAFDFF 68
           S+K  AV   IP ID+S+ R++ S  IV+AC+  GFFKVINHGV    I+ +E+++ +FF
Sbjct: 5   SQKIVAVDQDIPIIDMSQERSQVSMQIVKACESLGFFKVINHGVDQTTISRMEQESINFF 64

Query: 69  GQTAPEKRRAGPA-APFGYGCKAIGPNGDIGDLEYILLH-SDPASIAETSASISNSPLAF 128
            + A EK+   P   PF YG + IG NGD G++EY+L H +DPA           S L+F
Sbjct: 65  AKPAHEKKSVRPVNQPFRYGFRDIGLNGDSGEVEYLLFHTNDPA---------FRSQLSF 124

Query: 129 SYVVNEYVKSVRELACEILDLVGEGLWLPNKSVFSNLIRDVQSDSVLRINYYPAAVKHTE 188
           S  VN Y+++V++LA EILDL  EGL +P  S FS LI  V SDSVLR+N+YP + +   
Sbjct: 125 SSAVNCYIEAVKQLAREILDLTAEGLHVPPHS-FSRLISSVDSDSVLRVNHYPPSDQFFG 184

Query: 189 KSRELSSKSHLSTNNIGFGEHSDPQILTILRSNDVDGLQISLHDGLWTPVCPDPSAFYIL 248
           ++  LS +S +S   +GFGEH+DPQILT+LRSN V GLQ+S  DG+W  V PDPSAF + 
Sbjct: 185 EA-NLSDQS-VSLTRVGFGEHTDPQILTVLRSNGVGGLQVSNSDGMWVSVSPDPSAFCVN 244

Query: 249 IGDAFQAMTNDRFVSVRHRAMANNSKKARMSIMYFGAPPLNAWIAPPSDMMLPKDQ-SLY 308
           +GD  Q MTN RF+SVRHRA+    +++R+S  YF  PPL A I P S M++  +Q  LY
Sbjct: 245 VGDLLQVMTNGRFISVRHRAL-TYGEESRLSTAYFAGPPLQAKIGPLSAMVMTMNQPRLY 304

Query: 309 KPFTWAQYKKAAYSLRLADSRLHLFK 330
           + FTW +YKK AYSLRL DSRL +F+
Sbjct: 305 QTFTWGEYKKRAYSLRLEDSRLDMFR 317

[Close]

BLAST of CmaCh08G004850 vs. ExPASy Swiss-Prot
Match: Q9XFR9 (Gibberellin 2-beta-dioxygenase 2 OS=Arabidopsis thaliana OX=3702 GN=GA2OX2 PE=2 SV=1)

HSP 1 Score: 290.0 bits (741), Expect = 3.3e-77
Identity = 146/314 (46.50%), Postives = 212/314 (67.52%), Query Frame = 0


Query: 17  IPTIDLSESRARTSKSIVEACQHFGFFKVINHGVSDHAIANLERQAFDFFGQTAPEKRRA 76
           IP ++L++  A+T   IV+AC+ FGFFKV+NHGV    +  LE++A  FFG     K RA
Sbjct: 31  IPVVNLADPEAKT--RIVKACEEFGFFKVVNHGVRPELMTRLEQEAIGFFGLPQSLKNRA 90

Query: 77  GPAAPFGYGCKAIGPNGDIGDLEYILLHSDP-ASIAETSASISNSPLAFSYVVNEYVKSV 136
           GP  P+GYG K IGPNGD+G +EY+LL+++P  S  +TSA    +P  F   V EY+K +
Sbjct: 91  GPPEPYGYGNKRIGPNGDVGWIEYLLLNANPQLSSPKTSAVFRQTPQIFRESVEEYMKEI 150

Query: 137 RELACEILDLVGEGLWLPNKSVFSNLIRDVQSDSVLRINYYPAAVKHTEKSRELSSKSHL 196
           +E++ ++L++V E L +  +   S ++RD +SDS LR+N+YPAA +  EK  +       
Sbjct: 151 KEVSYKVLEMVAEELGIEPRDTLSKMLRDEKSDSCLRLNHYPAAEEEAEKMVK------- 210

Query: 197 STNNIGFGEHSDPQILTILRSNDVDGLQISLHDGLWTPVCPDPSAFYILIGDAFQAMTND 256
               +GFGEH+DPQI+++LRSN+  GLQI + DG W  V PD S+F+I +GDA Q MTN 
Sbjct: 211 ----VGFGEHTDPQIISVLRSNNTAGLQICVKDGSWVAVPPDHSSFFINVGDALQVMTNG 270

Query: 257 RFVSVRHRAMANNSKKARMSIMYFGAPPLNAWIAPPSDMMLPKDQSLYKPFTWAQYKKAA 316
           RF SV+HR +A +++++R+S++YFG PPL+  IAP   ++  +D  LYK FTW+QYK +A
Sbjct: 271 RFKSVKHRVLA-DTRRSRISMIYFGGPPLSQKIAPLPCLVPEQDDWLYKEFTWSQYKSSA 330

Query: 317 YSLRLADSRLHLFK 330
           Y  +L D RL LF+
Sbjct: 331 YKSKLGDYRLGLFE 330

[Close]

BLAST of CmaCh08G004850 vs. ExPASy Swiss-Prot
Match: Q9XG83 (Gibberellin 2-beta-dioxygenase OS=Phaseolus coccineus OX=3886 GN=GA2OX1 PE=2 SV=1)

HSP 1 Score: 288.5 bits (737), Expect = 9.6e-77
Identity = 149/317 (47.00%), Postives = 208/317 (65.62%), Query Frame = 0


Query: 16  GIPTIDLSESRARTSKSIVEACQHFGFFKVINHGVSDHAIANLERQAFDFFGQTAPEKRR 75
           GIP +DL+   A+    IV AC+ FGFFK++NHGV    +ANLE +A  FF ++  EK R
Sbjct: 26  GIPVVDLTHPDAK--NLIVNACRDFGFFKLVNHGVPLELMANLENEALRFFKKSQSEKDR 85

Query: 76  AGPAAPFGYGCKAIGPNGDIGDLEYILLHSDPASIAETSASI-SNSPLAFSYVVNEYVKS 135
           AGP  PFGYG K IGPNGD+G +EY+LL+++P  I+  S  I   +P  F  VV  Y+ +
Sbjct: 86  AGPPDPFGYGSKRIGPNGDVGWVEYLLLNTNPDVISPKSLCIFRENPHHFRAVVENYITA 145

Query: 136 VRELACEILDLVGEGLWLPNKSVFSNLIRDVQSDSVLRINYYPAAVKHTEKSRELSSKSH 195
           V+ +   +L+L+ EGL +  ++  S L++D +SDS  R+N+YP                 
Sbjct: 146 VKNMCYAVLELMAEGLGIRQRNTLSRLLKDEKSDSCFRLNHYPPC----------PEVQA 205

Query: 196 LSTNNIGFGEHSDPQILTILRSNDVDGLQISLHDGLWTPVCPDPSAFYILIGDAFQAMTN 255
           L+ N +GFGEH+DPQI+++LRSN   GLQI L DG W  V PD ++F+I +GDA Q MTN
Sbjct: 206 LNRNLVGFGEHTDPQIISVLRSNSTSGLQICLTDGTWVSVPPDQTSFFINVGDALQVMTN 265

Query: 256 DRFVSVRHRAMANNSKKARMSIMYFGAPPLNAWIAPPSDMMLPKDQSLYKPFTWAQYKKA 315
            RF SV+HR +A+ + K+R+S++YFG P L+  IAP   +ML  ++ LYK FTW +YKKA
Sbjct: 266 GRFKSVKHRVLADTT-KSRLSMIYFGGPALSENIAPLPSVMLKGEECLYKEFTWCEYKKA 325

Query: 316 AYSLRLADSRLHLFKAS 332
           AY+ RLAD+RL  F+ S
Sbjct: 326 AYTSRLADNRLAPFQKS 329

[Close]

BLAST of CmaCh08G004850 vs. TAIR 10
Match: AT1G02400.1 (gibberellin 2-oxidase 6 )

HSP 1 Score: 337.4 bits (864), Expect = 1.3e-92
Identity = 173/331 (52.27%), Postives = 218/331 (65.86%), Query Frame = 0


Query: 4   PTATRSKKT---KAVGIPTIDLS-ESRARTSKSIVEACQHFGFFKVINHGVSDHAIANLE 63
           P  T  KKT        P ID S   R++ S+ IV+AC+  GFFKVINHGV    I   E
Sbjct: 8   PLQTTGKKTISSPEYNFPVIDFSLNDRSKLSEKIVKACEVNGFFKVINHGVKPEIIKRFE 67

Query: 64  RQAFDFFGQTAPEKRRAGPAAPFGYGCKAIGPNGDIGDLEYILLHSDPASIAETSASIS- 123
            +  +FF +   +K RAGPA+PFGYGCK IG NGD+G+LEY+LLH++P ++A+ S +IS 
Sbjct: 68  HEGEEFFNKPESDKLRAGPASPFGYGCKNIGFNGDLGELEYLLLHANPTAVADKSETISH 127

Query: 124 NSPLAFSYVVNEYVKSVRELACEILDLVGEGLWLPNKSVFSNLIRDVQSDSVLRINYYPA 183
           + P  FS   N+Y+++VR+LACEI+DL  E LW    S  S LIRDV+SDS+LR+N+YP 
Sbjct: 128 DDPFKFSSATNDYIRTVRDLACEIIDLTIENLWGQKSSEVSELIRDVRSDSILRLNHYPP 187

Query: 184 AVKHTEKSRELSSKSHLSTNNIGFGEHSDPQILTILRSNDVDGLQISLHDGLWTPVCPDP 243
           A             +      IGFGEHSDPQILT+LRSNDVDGL+I   DGLW P+  DP
Sbjct: 188 A-----------PYALSGVGQIGFGEHSDPQILTVLRSNDVDGLEICSRDGLWIPIPSDP 247

Query: 244 SAFYILIGDAFQAMTNDRFVSVRHRAMANNSKKARMSIMYFGAPPLNAWIAPPSDMMLPK 303
           + F++L+GD  QA+TN RF SVRHR +AN +KK RMS MYF APPL A I+P   M+ P+
Sbjct: 248 TCFFVLVGDCLQALTNGRFTSVRHRVLANTAKKPRMSAMYFAAPPLEAKISPLPKMVSPE 307

Query: 304 DQSLYKPFTWAQYKKAAYSLRLADSRLHLFK 330
           +   Y  FTW  YKKA YSLRL   RL  FK
Sbjct: 308 NPRRYNSFTWGDYKKATYSLRLDVPRLEFFK 327

[Close]

BLAST of CmaCh08G004850 vs. TAIR 10
Match: AT1G47990.1 (gibberellin 2-oxidase 4 )

HSP 1 Score: 292.7 bits (748), Expect = 3.6e-79
Identity = 164/326 (50.31%), Postives = 219/326 (67.18%), Query Frame = 0


Query: 9   SKKTKAV--GIPTIDLSESRARTSKSIVEACQHFGFFKVINHGVSDHAIANLERQAFDFF 68
           S+K  AV   IP ID+S+ R++ S  IV+AC+  GFFKVINHGV    I+ +E+++ +FF
Sbjct: 5   SQKIVAVDQDIPIIDMSQERSQVSMQIVKACESLGFFKVINHGVDQTTISRMEQESINFF 64

Query: 69  GQTAPEKRRAGPA-APFGYGCKAIGPNGDIGDLEYILLH-SDPASIAETSASISNSPLAF 128
            + A EK+   P   PF YG + IG NGD G++EY+L H +DPA           S L+F
Sbjct: 65  AKPAHEKKSVRPVNQPFRYGFRDIGLNGDSGEVEYLLFHTNDPA---------FRSQLSF 124

Query: 129 SYVVNEYVKSVRELACEILDLVGEGLWLPNKSVFSNLIRDVQSDSVLRINYYPAAVKHTE 188
           S  VN Y+++V++LA EILDL  EGL +P  S FS LI  V SDSVLR+N+YP + +   
Sbjct: 125 SSAVNCYIEAVKQLAREILDLTAEGLHVPPHS-FSRLISSVDSDSVLRVNHYPPSDQFFG 184

Query: 189 KSRELSSKSHLSTNNIGFGEHSDPQILTILRSNDVDGLQISLHDGLWTPVCPDPSAFYIL 248
           ++  LS +S +S   +GFGEH+DPQILT+LRSN V GLQ+S  DG+W  V PDPSAF + 
Sbjct: 185 EA-NLSDQS-VSLTRVGFGEHTDPQILTVLRSNGVGGLQVSNSDGMWVSVSPDPSAFCVN 244

Query: 249 IGDAFQAMTNDRFVSVRHRAMANNSKKARMSIMYFGAPPLNAWIAPPSDMMLPKDQ-SLY 308
           +GD  Q MTN RF+SVRHRA+    +++R+S  YF  PPL A I P S M++  +Q  LY
Sbjct: 245 VGDLLQVMTNGRFISVRHRAL-TYGEESRLSTAYFAGPPLQAKIGPLSAMVMTMNQPRLY 304

Query: 309 KPFTWAQYKKAAYSLRLADSRLHLFK 330
           + FTW +YKK AYSLRL DSRL +F+
Sbjct: 305 QTFTWGEYKKRAYSLRLEDSRLDMFR 317

[Close]

BLAST of CmaCh08G004850 vs. TAIR 10
Match: AT1G30040.1 (gibberellin 2-oxidase )

HSP 1 Score: 290.0 bits (741), Expect = 2.3e-78
Identity = 146/314 (46.50%), Postives = 212/314 (67.52%), Query Frame = 0


Query: 17  IPTIDLSESRARTSKSIVEACQHFGFFKVINHGVSDHAIANLERQAFDFFGQTAPEKRRA 76
           IP ++L++  A+T   IV+AC+ FGFFKV+NHGV    +  LE++A  FFG     K RA
Sbjct: 31  IPVVNLADPEAKT--RIVKACEEFGFFKVVNHGVRPELMTRLEQEAIGFFGLPQSLKNRA 90

Query: 77  GPAAPFGYGCKAIGPNGDIGDLEYILLHSDP-ASIAETSASISNSPLAFSYVVNEYVKSV 136
           GP  P+GYG K IGPNGD+G +EY+LL+++P  S  +TSA    +P  F   V EY+K +
Sbjct: 91  GPPEPYGYGNKRIGPNGDVGWIEYLLLNANPQLSSPKTSAVFRQTPQIFRESVEEYMKEI 150

Query: 137 RELACEILDLVGEGLWLPNKSVFSNLIRDVQSDSVLRINYYPAAVKHTEKSRELSSKSHL 196
           +E++ ++L++V E L +  +   S ++RD +SDS LR+N+YPAA +  EK  +       
Sbjct: 151 KEVSYKVLEMVAEELGIEPRDTLSKMLRDEKSDSCLRLNHYPAAEEEAEKMVK------- 210

Query: 197 STNNIGFGEHSDPQILTILRSNDVDGLQISLHDGLWTPVCPDPSAFYILIGDAFQAMTND 256
               +GFGEH+DPQI+++LRSN+  GLQI + DG W  V PD S+F+I +GDA Q MTN 
Sbjct: 211 ----VGFGEHTDPQIISVLRSNNTAGLQICVKDGSWVAVPPDHSSFFINVGDALQVMTNG 270

Query: 257 RFVSVRHRAMANNSKKARMSIMYFGAPPLNAWIAPPSDMMLPKDQSLYKPFTWAQYKKAA 316
           RF SV+HR +A +++++R+S++YFG PPL+  IAP   ++  +D  LYK FTW+QYK +A
Sbjct: 271 RFKSVKHRVLA-DTRRSRISMIYFGGPPLSQKIAPLPCLVPEQDDWLYKEFTWSQYKSSA 330

Query: 317 YSLRLADSRLHLFK 330
           Y  +L D RL LF+
Sbjct: 331 YKSKLGDYRLGLFE 330

[Close]

BLAST of CmaCh08G004850 vs. TAIR 10
Match: AT2G34555.1 (gibberellin 2-oxidase 3 )

HSP 1 Score: 279.3 bits (713), Expect = 4.1e-75
Identity = 146/320 (45.62%), Postives = 207/320 (64.69%), Query Frame = 0


Query: 11  KTKAVGIPTIDLSESRARTSKSIVEACQHFGFFKVINHGVSDHAIANLERQAFDFFGQTA 70
           K + V IP IDL++S A+T   IV+AC+ FGFFKVINHGV    +  LE++A +FF    
Sbjct: 21  KPRPVLIPVIDLTDSDAKT--QIVKACEEFGFFKVINHGVRPDLLTQLEQEAINFFALHH 80

Query: 71  PEKRRAGPAAPFGYGCKAIGPNGDIGDLEYILLHSDPA-SIAETSASISNSPLAFSYVVN 130
             K +AGP  PFGYG K IGPNGD+G LEYILL+++      +T+A   ++P  F   V 
Sbjct: 81  SLKDKAGPPDPFGYGTKRIGPNGDLGWLEYILLNANLCLESHKTTAIFRHTPAIFREAVE 140

Query: 131 EYVKSVRELACEILDLVGEGLWLPNKSVFSNLIRDVQSDSVLRINYYPAAVKHTEKSREL 190
           EY+K ++ ++ + L++V E L +  K   S L++  +SDS LR+N+YP   +   K    
Sbjct: 141 EYIKEMKRMSSKFLEMVEEELKIEPKEKLSRLVKVKESDSCLRMNHYPEKEETPVK---- 200

Query: 191 SSKSHLSTNNIGFGEHSDPQILTILRSNDVDGLQISLHDGLWTPVCPDPSAFYILIGDAF 250
                     IGFGEH+DPQ++++LRSND +GLQI + DG W  V PD S+F++L+GD  
Sbjct: 201 --------EEIGFGEHTDPQLISLLRSNDTEGLQICVKDGTWVDVTPDHSSFFVLVGDTL 260

Query: 251 QAMTNDRFVSVRHRAMANNSKKARMSIMYFGAPPLNAWIAPPSDMMLPKDQSLYKPFTWA 310
           Q MTN RF SV+HR +  N+K++R+S++YF  PPL+  IAP S ++  +D  LY  FTW+
Sbjct: 261 QVMTNGRFKSVKHR-VVTNTKRSRISMIYFAGPPLSEKIAPLSCLVPKQDDCLYNEFTWS 320

Query: 311 QYKKAAYSLRLADSRLHLFK 330
           QYK +AY  +L D RL LF+
Sbjct: 321 QYKLSAYKTKLGDYRLGLFE 325

[Close]

BLAST of CmaCh08G004850 vs. TAIR 10
Match: AT1G78440.1 (Arabidopsis thaliana gibberellin 2-oxidase 1 )

HSP 1 Score: 256.9 bits (655), Expect = 2.2e-68
Identity = 132/314 (42.04%), Postives = 201/314 (64.01%), Query Frame = 0


Query: 17  IPTIDLSESRARTSKSIVEACQHFGFFKVINHGVSDHAIANLERQAFDFFGQTAPEKRRA 76
           IP ID+S+  ++   ++V+AC+ FGFFKVINHGVS   ++ LE +  DFF     EK + 
Sbjct: 18  IPVIDMSDPESK--HALVKACEDFGFFKVINHGVSAELVSVLEHETVDFFSLPKSEKTQV 77

Query: 77  GPAAPFGYGCKAIGPNGDIGDLEYILLHSD-PASIAETSASISNSPLAFSYVVNEYVKSV 136
               PFGYG   IG NGD+G +EY+L++++  +       S+  SP  F   + EY  SV
Sbjct: 78  A-GYPFGYGNSKIGRNGDVGWVEYLLMNANHDSGSGPLFPSLLKSPGTFRNALEEYTTSV 137

Query: 137 RELACEILDLVGEGLWLPNKSVFSNLIRDVQSDSVLRINYYPAAVKHTEKSRELSSKSHL 196
           R++  ++L+ + +GL +  ++  S L+ D  +DS+LR+N+YP      +K+         
Sbjct: 138 RKMTFDVLEKITDGLGIKPRNTLSKLVSDQNTDSILRLNHYPPCPLSNKKTNG------- 197

Query: 197 STNNIGFGEHSDPQILTILRSNDVDGLQISLHDGLWTPVCPDPSAFYILIGDAFQAMTND 256
             N IGFGEH+DPQI+++LRSN+  GLQI+L+DG W  V PD ++F+  +GD+ Q MTN 
Sbjct: 198 GKNVIGFGEHTDPQIISVLRSNNTSGLQINLNDGSWISVPPDHTSFFFNVGDSLQVMTNG 257

Query: 257 RFVSVRHRAMANNSKKARMSIMYFGAPPLNAWIAPPSDMMLPKDQSLYKPFTWAQYKKAA 316
           RF SVRHR +A N KK+R+S++YF  P L   IAP + ++  +D+ LY+ FTW++YK + 
Sbjct: 258 RFKSVRHRVLA-NCKKSRVSMIYFAGPSLTQRIAPLTCLIDNEDERLYEEFTWSEYKNST 317

Query: 317 YSLRLADSRLHLFK 330
           Y+ RL+D+RL  F+
Sbjct: 318 YNSRLSDNRLQQFE 320

The following BLAST results are available for this feature:

BLAST of CmaCh08G004850 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9XHM5	2.1e-108	58.01	Gibberellin 2-beta-dioxygenase 2 OS=Pisum sativum OX=3888 GN=GA2OX2 PE=2 SV=1	[more]
Q9FZ21	1.8e-91	52.27	Gibberellin 2-beta-dioxygenase 6 OS=Arabidopsis thaliana OX=3702 GN=GA2OX6 PE=2 ...	[more]
Q9C7Z1	5.1e-78	50.31	Gibberellin 2-beta-dioxygenase 4 OS=Arabidopsis thaliana OX=3702 GN=GA2OX4 PE=2 ...	[more]
Q9XFR9	3.3e-77	46.50	Gibberellin 2-beta-dioxygenase 2 OS=Arabidopsis thaliana OX=3702 GN=GA2OX2 PE=2 ...	[more]
Q9XG83	9.6e-77	47.00	Gibberellin 2-beta-dioxygenase OS=Phaseolus coccineus OX=3886 GN=GA2OX1 PE=2 SV=...	[more]

BLAST of CmaCh08G004850 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G02400.1	1.3e-92	52.27	gibberellin 2-oxidase 6	[more]
AT1G47990.1	3.6e-79	50.31	gibberellin 2-oxidase 4	[more]
AT1G30040.1	2.3e-78	46.50	gibberellin 2-oxidase	[more]
AT2G34555.1	4.1e-75	45.63	gibberellin 2-oxidase 3	[more]
AT1G78440.1	2.2e-68	42.04	Arabidopsis thaliana gibberellin 2-oxidase 1	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PRINTS	PR00682	IPNSYNTHASE	coord: 33..50 score: 51.01 coord: 205..226 score: 32.23 coord: 241..267 score: 26.26
None	No IPR available	PANTHER	PTHR47990	2-OXOGLUTARATE (2OG) AND FE(II)-DEPENDENT OXYGENASE SUPERFAMILY PROTEIN-RELATED	coord: 4..329
None	No IPR available	PANTHER	PTHR47990:SF45	GIBBERELLIN 2-BETA-DIOXYGENASE 6	coord: 4..329
None	No IPR available	SUPERFAMILY	51197	Clavaminate synthase-like	coord: 10..321
IPR044861	Isopenicillin N synthase-like, Fe(2+) 2OG dioxygenase domain	PFAM	PF03171	2OG-FeII_Oxy	coord: 168..280 e-value: 6.4E-21 score: 74.7
IPR026992	Non-haem dioxygenase N-terminal domain	PFAM	PF14226	DIOX_N	coord: 17..81 e-value: 3.8E-14 score: 53.4
IPR027443	Isopenicillin N synthase-like superfamily	GENE3D	2.60.120.330		coord: 3..319 e-value: 1.4E-68 score: 233.6
IPR005123	Oxoglutarate/iron-dependent dioxygenase	PROSITE	PS51471	FE2OG_OXY	coord: 166..282 score: 12.141252

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmaCh08G004850.1	CmaCh08G004850.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0045487	gibberellin catabolic process
biological_process	GO:0009416	response to light stimulus
molecular_function	GO:0052634	C-19 gibberellin 2-beta-dioxygenase activity
molecular_function	GO:0046872	metal ion binding