CmaCh08G004420 (gene) Cucurbita maxima (Rimu) v1.1

Overview


Name	CmaCh08G004420
Type	gene
Organism	Cucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Description	Pectinesterase
Location	Cma_Chr08: 2494265 .. 2496238 (+)
RNA-Seq Expression	CmaCh08G004420
Synteny	CmaCh08G004420

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

AAACCTCAAAACAAACCACCACTTCTCTCTCCAATGGCGGCCACCACATTCTCCTCTCTCATCCTCCTCTCCCTCTACATAACCACCGCTCAAATTCCCCTCTCCGCCGCCTCCGTCTGCACCTCCACCCCCGATCCCTCCTACTGCAAATCCGCCCTCCCCAACCAAACCGGCAATGTCTATTCATACGGCCGCTCATCTTTCAGAAAATCCCTCTCATCGTCGCAAAAGTTTCTCCGGCTAGTCAAGAAGTACCTCCGGTCACGCTCGTCGTTGACGGTTCCCGCCGTCGGAGCACTGGAGGACTGCAAGCTGTTGGCGGGACTGAATATTGATTACTTAACGACGTCGTTCCGGGCGGTGAATACGACGAGTTCGAAGCTGCCGGAGATGAAGGCGGACGATGTACAGAGTTTGTTGAGTGCGATATTGACGAACCAGCAGACGTGTTTGGATGGGCTCAAGGCGACGGCGGGTTCGTGGTCTGTCAAAAATGGCCTTTCCCTCCCACTTGCCTCTGACACCAAGCTGTACTCTATGTCCTTGGCTTTATTTACCAAAGGTTTATATATATATTTATATATATACTTTTTTTTTTAATTTTATTTATTTATTATATTTAATTAATATAGAGTCGTGATTTTTAATAATTAATAAGTTATTAAAATCAAATATAATTCAAAATAATATATATTTATTATATATATTGATTTCCCGTAGGTTGGGTTCCGAAGAAGAAGAAACGGTCGACATGGACGGCGGCCGGGAGTCTGGGCGGGTTCAGAAACGGAAGAATGTCGCTCAAAATGTCGAACAGAGCGAAAGCGATTTACGAGATGGCGACGCGCCGGAATCTGCTGGAGACTGACGACGGCGGCGACGACCAGATTTCGATCAGAGACATCGTCATTGTCAGCCAAGACGGCAGCGGGAATTTCACGACTATTAACGAAGCGATTGCGGCGGCGCCGAATAACTCTGCTCCGACCGACGGCTATTTCTTGATCTTTGTGTCGGTCGGAGTTTACGAGGAATACGTGTCGATTGCGAAGAATAAGCGGTATTTGATGATGATCGGAGATGGCATTAATCAGACCGTTATCACTGGTAATCGGAGCGTCGTTGACGGCTGGACCACTTTCAATTCTGCCACGTTTGGTTAGTGTTTGATTAATTAGTTGAATTTTCAATTTTGTAATTAATAATTTCATTTTCAAGTTGGTTATTAATAAATTTTAAATGTCCTAAATAGATTTTTTTTTTTTTTTTTTACCAGCGGTGGTGGGACCAGGATTTGTAGCGGTAAACATGACGTTCAGAAACACCGCCGGAGCAATAAAGCACCAAGCCGTCGCCGTCCGTAACGGCGCCGACTTATCTACTTTCTATCTCTGCAGCTTCGAAGGCTACCAAGACACCTTATACACTCACTCCCTCCGCCAATTTTACCGCGACTGCGACATCTACGGCACAGTCGACTTCATCTTCGGCAACGCCGCCGTCGTTTTCCAGAACTGCAACATCTACCCTCGTCTTCCTATGGCGAACCAGTTCAACGCCATCACTGCTCAGGGCCGGACCGACCCAAACCAGAACACCGGAACCTCCATCCATAACTGCCGGATCACCGCCGCTGACGACCTCGCGAACAACACCGGCGTCGCCGTGAAAACGTTCCTCGGACGACCGTGGAAGGAATATTCAAGGACGGTGTATTTGCAGAGCTTCATGGATGAGCTAATTAATCCGGCGGGGTGGAGGGCTTGGGATAAGGATTTTGCTTTGAACACTTCGTATTATGCTGAATTTGGGAATTTTGGGCCGGGATCTAACACGTCGGAGAGGGTGAATTGGCTGGGATTTCATTTGATTAATGATACCGATGCTGGGAATTTCACGGCGGCGAACTTTGTCTTGGCGGATGATTGGCTGCCGCAGACCGGCGTTCCGTACACCAGCGGCTTGACGGAGTAG

mRNA sequence

AAACCTCAAAACAAACCACCACTTCTCTCTCCAATGGCGGCCACCACATTCTCCTCTCTCATCCTCCTCTCCCTCTACATAACCACCGCTCAAATTCCCCTCTCCGCCGCCTCCGTCTGCACCTCCACCCCCGATCCCTCCTACTGCAAATCCGCCCTCCCCAACCAAACCGGCAATGTCTATTCATACGGCCGCTCATCTTTCAGAAAATCCCTCTCATCGTCGCAAAAGTTTCTCCGGCTAGTCAAGAAGTACCTCCGGTCACGCTCGTCGTTGACGGTTCCCGCCGTCGGAGCACTGGAGGACTGCAAGCTGTTGGCGGGACTGAATATTGATTACTTAACGACGTCGTTCCGGGCGGTGAATACGACGAGTTCGAAGCTGCCGGAGATGAAGGCGGACGATGTACAGAGTTTGTTGAGTGCGATATTGACGAACCAGCAGACGTGTTTGGATGGGCTCAAGGCGACGGCGGGTTCGTGGTCTGTCAAAAATGGCCTTTCCCTCCCACTTGCCTCTGACACCAAGCTGTACTCTATGTCCTTGGCTTTATTTACCAAAGGTTGGGTTCCGAAGAAGAAGAAACGGTCGACATGGACGGCGGCCGGGAGTCTGGGCGGGTTCAGAAACGGAAGAATGTCGCTCAAAATGTCGAACAGAGCGAAAGCGATTTACGAGATGGCGACGCGCCGGAATCTGCTGGAGACTGACGACGGCGGCGACGACCAGATTTCGATCAGAGACATCGTCATTGTCAGCCAAGACGGCAGCGGGAATTTCACGACTATTAACGAAGCGATTGCGGCGGCGCCGAATAACTCTGCTCCGACCGACGGCTATTTCTTGATCTTTGTGTCGGTCGGAGTTTACGAGGAATACGTGTCGATTGCGAAGAATAAGCGGTATTTGATGATGATCGGAGATGGCATTAATCAGACCGTTATCACTGGTAATCGGAGCGTCGTTGACGGCTGGACCACTTTCAATTCTGCCACGTTTGCGGTGGTGGGACCAGGATTTGTAGCGGTAAACATGACGTTCAGAAACACCGCCGGAGCAATAAAGCACCAAGCCGTCGCCGTCCGTAACGGCGCCGACTTATCTACTTTCTATCTCTGCAGCTTCGAAGGCTACCAAGACACCTTATACACTCACTCCCTCCGCCAATTTTACCGCGACTGCGACATCTACGGCACAGTCGACTTCATCTTCGGCAACGCCGCCGTCGTTTTCCAGAACTGCAACATCTACCCTCGTCTTCCTATGGCGAACCAGTTCAACGCCATCACTGCTCAGGGCCGGACCGACCCAAACCAGAACACCGGAACCTCCATCCATAACTGCCGGATCACCGCCGCTGACGACCTCGCGAACAACACCGGCGTCGCCGTGAAAACGTTCCTCGGACGACCGTGGAAGGAATATTCAAGGACGGTGTATTTGCAGAGCTTCATGGATGAGCTAATTAATCCGGCGGGGTGGAGGGCTTGGGATAAGGATTTTGCTTTGAACACTTCGTATTATGCTGAATTTGGGAATTTTGGGCCGGGATCTAACACGTCGGAGAGGGTGAATTGGCTGGGATTTCATTTGATTAATGATACCGATGCTGGGAATTTCACGGCGGCGAACTTTGTCTTGGCGGATGATTGGCTGCCGCAGACCGGCGTTCCGTACACCAGCGGCTTGACGGAGTAG

Coding sequence (CDS)

ATGGCGGCCACCACATTCTCCTCTCTCATCCTCCTCTCCCTCTACATAACCACCGCTCAAATTCCCCTCTCCGCCGCCTCCGTCTGCACCTCCACCCCCGATCCCTCCTACTGCAAATCCGCCCTCCCCAACCAAACCGGCAATGTCTATTCATACGGCCGCTCATCTTTCAGAAAATCCCTCTCATCGTCGCAAAAGTTTCTCCGGCTAGTCAAGAAGTACCTCCGGTCACGCTCGTCGTTGACGGTTCCCGCCGTCGGAGCACTGGAGGACTGCAAGCTGTTGGCGGGACTGAATATTGATTACTTAACGACGTCGTTCCGGGCGGTGAATACGACGAGTTCGAAGCTGCCGGAGATGAAGGCGGACGATGTACAGAGTTTGTTGAGTGCGATATTGACGAACCAGCAGACGTGTTTGGATGGGCTCAAGGCGACGGCGGGTTCGTGGTCTGTCAAAAATGGCCTTTCCCTCCCACTTGCCTCTGACACCAAGCTGTACTCTATGTCCTTGGCTTTATTTACCAAAGGTTGGGTTCCGAAGAAGAAGAAACGGTCGACATGGACGGCGGCCGGGAGTCTGGGCGGGTTCAGAAACGGAAGAATGTCGCTCAAAATGTCGAACAGAGCGAAAGCGATTTACGAGATGGCGACGCGCCGGAATCTGCTGGAGACTGACGACGGCGGCGACGACCAGATTTCGATCAGAGACATCGTCATTGTCAGCCAAGACGGCAGCGGGAATTTCACGACTATTAACGAAGCGATTGCGGCGGCGCCGAATAACTCTGCTCCGACCGACGGCTATTTCTTGATCTTTGTGTCGGTCGGAGTTTACGAGGAATACGTGTCGATTGCGAAGAATAAGCGGTATTTGATGATGATCGGAGATGGCATTAATCAGACCGTTATCACTGGTAATCGGAGCGTCGTTGACGGCTGGACCACTTTCAATTCTGCCACGTTTGCGGTGGTGGGACCAGGATTTGTAGCGGTAAACATGACGTTCAGAAACACCGCCGGAGCAATAAAGCACCAAGCCGTCGCCGTCCGTAACGGCGCCGACTTATCTACTTTCTATCTCTGCAGCTTCGAAGGCTACCAAGACACCTTATACACTCACTCCCTCCGCCAATTTTACCGCGACTGCGACATCTACGGCACAGTCGACTTCATCTTCGGCAACGCCGCCGTCGTTTTCCAGAACTGCAACATCTACCCTCGTCTTCCTATGGCGAACCAGTTCAACGCCATCACTGCTCAGGGCCGGACCGACCCAAACCAGAACACCGGAACCTCCATCCATAACTGCCGGATCACCGCCGCTGACGACCTCGCGAACAACACCGGCGTCGCCGTGAAAACGTTCCTCGGACGACCGTGGAAGGAATATTCAAGGACGGTGTATTTGCAGAGCTTCATGGATGAGCTAATTAATCCGGCGGGGTGGAGGGCTTGGGATAAGGATTTTGCTTTGAACACTTCGTATTATGCTGAATTTGGGAATTTTGGGCCGGGATCTAACACGTCGGAGAGGGTGAATTGGCTGGGATTTCATTTGATTAATGATACCGATGCTGGGAATTTCACGGCGGCGAACTTTGTCTTGGCGGATGATTGGCTGCCGCAGACCGGCGTTCCGTACACCAGCGGCTTGACGGAGTAG

Protein sequence

MAATTFSSLILLSLYITTAQIPLSAASVCTSTPDPSYCKSALPNQTGNVYSYGRSSFRKSLSSSQKFLRLVKKYLRSRSSLTVPAVGALEDCKLLAGLNIDYLTTSFRAVNTTSSKLPEMKADDVQSLLSAILTNQQTCLDGLKATAGSWSVKNGLSLPLASDTKLYSMSLALFTKGWVPKKKKRSTWTAAGSLGGFRNGRMSLKMSNRAKAIYEMATRRNLLETDDGGDDQISIRDIVIVSQDGSGNFTTINEAIAAAPNNSAPTDGYFLIFVSVGVYEEYVSIAKNKRYLMMIGDGINQTVITGNRSVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVAVRNGADLSTFYLCSFEGYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNIYPRLPMANQFNAITAQGRTDPNQNTGTSIHNCRITAADDLANNTGVAVKTFLGRPWKEYSRTVYLQSFMDELINPAGWRAWDKDFALNTSYYAEFGNFGPGSNTSERVNWLGFHLINDTDAGNFTAANFVLADDWLPQTGVPYTSGLTE

Homology

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BLAST of CmaCh08G004420 vs. ExPASy Swiss-Prot
Match: Q9SRX4 (Probable pectinesterase/pectinesterase inhibitor 7 OS=Arabidopsis thaliana OX=3702 GN=PME7 PE=2 SV=1)

HSP 1 Score: 697.6 bits (1799), Expect = 1.1e-199
Identity = 353/571 (61.82%), Postives = 429/571 (75.13%), Query Frame = 0


Query: 9   LILLSLY----ITTAQIPLSAASVCTSTPDPSYCKSALPNQTGNVYSYGRSSFRKSLSSS 68
           LI LS +    + ++Q   +++++C +TPDP YCKS  P+  GNV  YG  S RKSLS S
Sbjct: 8   LITLSFFLQSVLASSQTLSNSSTICKTTPDPKYCKSVFPHSQGNVQQYGCFSIRKSLSQS 67

Query: 69  QKFLRLVKKYLRSRSSLTVPAV-GALEDCKLLAGLNIDYLTTSFRAVNTTSSK-----LP 128
           +KF+R V +Y++  + L+ PAV  AL+DC+ LAGL +DYL TSF  VN TS+K     L 
Sbjct: 68  RKFIRTVDRYIKRNAHLSQPAVIRALQDCRFLAGLTMDYLLTSFETVNDTSAKTSFKPLS 127

Query: 129 EMKADDVQSLLSAILTNQQTCLDGLKATA---GSWSVKNGLSLPLASDTKLYSMSLALFT 188
             KADD+Q+LLSA LTN+QTCL+GL   A    +W+V+ G++LPL +DTKL  +SLALFT
Sbjct: 128 FPKADDIQTLLSAALTNEQTCLEGLTTAASYSATWTVRTGVALPLVNDTKLLGVSLALFT 187

Query: 189 KGWVPKKKKRSTWTAAGSLGG----------FRNGRMSLKMSNRAKAIYEMATRRNLLET 248
           KGWVPKKKKR+ +  A    G          FRNG + LKM+ + KA+YE  +RR L + 
Sbjct: 188 KGWVPKKKKRAGFAWAQPRSGSSTHTKPFRLFRNGALPLKMTEKTKAVYESLSRRKLADG 247

Query: 249 DDGGD----DQISIRDIVIVSQDGSGNFTTINEAIAAAPNNSAPTDGYFLIFVSVGVYEE 308
           D  GD      + I DIV VSQDG+GNFT I  A+AAAPNN+  + G+FLI+V+ G+YEE
Sbjct: 248 DSNGDGDDGSMVLISDIVTVSQDGTGNFTNITAAVAAAPNNTDGSAGFFLIYVTAGIYEE 307

Query: 309 YVSIAKNKRYLMMIGDGINQTVITGNRSVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAG 368
           Y+SIAKNKRY+MMIGDGINQTV+TGNRSVVDGWTTFNSATFAV  P FVAVN+TFRNTAG
Sbjct: 308 YISIAKNKRYMMMIGDGINQTVVTGNRSVVDGWTTFNSATFAVTAPNFVAVNITFRNTAG 367

Query: 369 AIKHQAVAVRNGADLSTFYLCSFEGYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQ 428
             KHQAVA+R+GAD S FY CSFE YQDTLYTHSLRQFYR+CD+YGTVDFIFGNAAVVFQ
Sbjct: 368 PEKHQAVALRSGADFSIFYSCSFEAYQDTLYTHSLRQFYRECDVYGTVDFIFGNAAVVFQ 427

Query: 429 NCNIYPRLPMANQFNAITAQGRTDPNQNTGTSIHNCRITAADDLANNTGVAVKTFLGRPW 488
           NCN+YPR PM NQFNAITAQGR+DPNQNTGTSI NC I  ADDL  ++   VKT+LGRPW
Sbjct: 428 NCNLYPRKPMPNQFNAITAQGRSDPNQNTGTSIQNCTIKPADDLV-SSNYTVKTYLGRPW 487

Query: 489 KEYSRTVYLQSFMDELINPAGWRAWDKDFALNTSYYAEFGNFGPGSNTSERVNWLGFHLI 548
           KEYSRTVY+QS++D  + P GWR W+ DFAL+T YYAE+ N GPGSNT+ RV W G+H+I
Sbjct: 488 KEYSRTVYMQSYIDGFVEPVGWREWNGDFALSTLYYAEYNNTGPGSNTTNRVTWPGYHVI 547

Query: 549 NDTDAGNFTAANFVLADDWLPQTGVPYTSGL 553
           N TDA NFT     +  DW+ +TGVPYTSGL
Sbjct: 548 NSTDAANFTVTGLFIEADWIWKTGVPYTSGL 577

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BLAST of CmaCh08G004420 vs. ExPASy Swiss-Prot
Match: Q8RXK7 (Probable pectinesterase/pectinesterase inhibitor 41 OS=Arabidopsis thaliana OX=3702 GN=PME41 PE=2 SV=2)

HSP 1 Score: 680.6 bits (1755), Expect = 1.5e-194
Identity = 350/564 (62.06%), Postives = 431/564 (76.42%), Query Frame = 0


Query: 4   TTFSSLILLSLYITTAQIPLSAASVCTSTPDPSYCKSALPNQT-GNVYSYGRSSFRKSLS 63
           T F SL  L +   T     S++++C +TPDP +CKS  P  + G+V  YGR S RKSL+
Sbjct: 10  TLFLSLQTLFIASQTLLPSNSSSTICKTTPDPKFCKSVFPQTSQGDVREYGRFSLRKSLT 69

Query: 64  SSQKFLRLVKKYL-RSRSSLTVPAVGALEDCKLLAGLNIDYLTTSFRAVN-TTSSK-LPE 123
            S+KF R + +YL R+ + L+  AVGAL+DC+ LA L  DYL TSF  VN TTSSK L  
Sbjct: 70  QSRKFTRTIDRYLKRNNALLSQSAVGALQDCRYLASLTTDYLITSFETVNITTSSKTLSF 129

Query: 124 MKADDVQSLLSAILTNQQTCLDGLK-ATAGSWSVKNGLSLPLASDTKLYSMSLALFTKGW 183
            KAD++Q+LLSA LTN+QTCLDG+  A + SW+++NG++LPL +DTKL+S+SLALFTKGW
Sbjct: 130 SKADEIQTLLSAALTNEQTCLDGINTAASSSWTIRNGVALPLINDTKLFSVSLALFTKGW 189

Query: 184 VPKKKK----------RSTWTAAGSLGGFRNGRMSLKMSNRAKAIYEMATRRNLLETDDG 243
           VPKKKK          ++T +       FRNG + LKM+   +A+YE  +RR L + DD 
Sbjct: 190 VPKKKKQVASYSWAHPKNTHSHTKPFRHFRNGALPLKMTEHTRAVYESLSRRKLAD-DDN 249

Query: 244 GDDQISIRDIVIVSQDGSGNFTTINEAIAAAPNNSAPTDGYFLIFVSVGVYEEYVSIAKN 303
             + + + DIV V+Q+G+GNFTTI EA+ +APN +  T GYF+I+V+ GVYEE V IAKN
Sbjct: 250 DVNTVLVSDIVTVNQNGTGNFTTITEAVNSAPNKTDGTAGYFVIYVTSGVYEENVVIAKN 309

Query: 304 KRYLMMIGDGINQTVITGNRSVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAV 363
           KRYLMMIGDGIN+TV+TGNR+VVDGWTTFNSATFAV  P FVAVNMTFRNTAG  KHQAV
Sbjct: 310 KRYLMMIGDGINRTVVTGNRNVVDGWTTFNSATFAVTSPNFVAVNMTFRNTAGPEKHQAV 369

Query: 364 AVRNGADLSTFYLCSFEGYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNIYPR 423
           A+R+ ADLS FY CSFE YQDTLYTHSLRQFYR+CDIYGTVDFIFGNAAVVFQ+CN+YPR
Sbjct: 370 AMRSSADLSIFYSCSFEAYQDTLYTHSLRQFYRECDIYGTVDFIFGNAAVVFQDCNLYPR 429

Query: 424 LPMANQFNAITAQGRTDPNQNTGTSIHNCRITAADDLANNTGVAVKTFLGRPWKEYSRTV 483
            PM NQFNAITAQGRTDPNQNTG SIHNC I  ADDL  ++   VKT+LGRPWKEYSRTV
Sbjct: 430 QPMQNQFNAITAQGRTDPNQNTGISIHNCTIKPADDLV-SSNYTVKTYLGRPWKEYSRTV 489

Query: 484 YLQSFMDELINPAGWRAWDKDFALNTSYYAEFGNFGPGSNTSERVNWLGFHLINDTDAGN 543
           ++QS++DE++ P GWR W+ DFAL+T YYAE+ N G GS+T++RV W G+H+IN TDA N
Sbjct: 490 FMQSYIDEVVEPVGWREWNGDFALSTLYYAEYNNTGSGSSTTDRVVWPGYHVINSTDANN 549

Query: 544 FTAANFVLADDWLPQTGVPYTSGL 553
           FT  NF+L D W+ Q+GVPY SGL
Sbjct: 550 FTVENFLLGDGWMVQSGVPYISGL 571

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BLAST of CmaCh08G004420 vs. ExPASy Swiss-Prot
Match: O22256 (Probable pectinesterase/pectinesterase inhibitor 20 OS=Arabidopsis thaliana OX=3702 GN=PME20 PE=2 SV=2)

HSP 1 Score: 646.7 bits (1667), Expect = 2.3e-184
Identity = 326/558 (58.42%), Postives = 413/558 (74.01%), Query Frame = 0


Query: 5   TFSSLILLSLYITTAQIP-----LSAASVCTSTPDPSYCKSALPNQTGNVYSYGRSSFRK 64
           T + L  L     +AQIP      S +++C   PDPSYC+S LPNQ G++YSYGR S R+
Sbjct: 10  TLACLSSLPSPFISAQIPAIGNATSPSNICRFAPDPSYCRSVLPNQPGDIYSYGRLSLRR 69

Query: 65  SLSSSQKFLRLVKKYLRSRSSLTVPA-VGALEDCKLLAGLNIDYLTTSFRAVNTTSSKLP 124
           SLS +++F+ ++   L  +  +   + VGALEDCK LA L +DYL +S +  ++T + L 
Sbjct: 70  SLSRARRFISMIDAELDRKGKVAAKSTVGALEDCKFLASLTMDYLLSSSQTADSTKT-LS 129

Query: 125 EMKADDVQSLLSAILTNQQTCLDGLKATAGSWSVKNGLSLPLASDTKLYSMSLALFTKGW 184
             +A+DV + LSA +TN+QTCL+GLK+TA     +NGLS  L +DTKLY +SLALF+KGW
Sbjct: 130 LSRAEDVHTFLSAAITNEQTCLEGLKSTAS----ENGLSGDLFNDTKLYGVSLALFSKGW 189

Query: 185 VPKKKK-RSTWTAAG---SLGGFRNGRMSLKMSNRAKAIYEMATRRNLLETDDGGDDQIS 244
           VP++++ R  W          GFRNG++ LKM+ RA+A+Y   TRR LL++D    D + 
Sbjct: 190 VPRRQRSRPIWQPQARFKKFFGFRNGKLPLKMTERARAVYNTVTRRKLLQSD---ADAVQ 249

Query: 245 IRDIVIVSQDGSGNFTTINEAIAAAPNNSAPTDGYFLIFVSVGVYEEYVSIAKNKRYLMM 304
           + DIV V Q+G+GNFTTIN AIAAAPN +  ++GYFLI+V+ G+YEEYV + KNKRY+MM
Sbjct: 250 VSDIVTVIQNGTGNFTTINAAIAAAPNKTDGSNGYFLIYVTAGLYEEYVEVPKNKRYVMM 309

Query: 305 IGDGINQTVITGNRSVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVAVRNGA 364
           IGDGINQTVITGNRSVVDGWTTFNSATF + GP F+ VN+T RNTAG  K QAVA+R+G 
Sbjct: 310 IGDGINQTVITGNRSVVDGWTTFNSATFILSGPNFIGVNITIRNTAGPTKGQAVALRSGG 369

Query: 365 DLSTFYLCSFEGYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNIYPRLPMANQ 424
           DLS FY CSFE YQDTLYTHSLRQFYR+CD+YGTVDFIFGNAAVV QNCN+YPR P   Q
Sbjct: 370 DLSVFYSCSFEAYQDTLYTHSLRQFYRECDVYGTVDFIFGNAAVVLQNCNLYPRQPRKGQ 429

Query: 425 FNAITAQGRTDPNQNTGTSIHNCRITAADDLANNTGVAVKTFLGRPWKEYSRTVYLQSFM 484
            N +TAQGRTDPNQNTGT+IH C I  ADDLA  +   VKT+LGRPWKEYSRTV +Q+++
Sbjct: 430 SNEVTAQGRTDPNQNTGTAIHGCTIRPADDLA-TSNYTVKTYLGRPWKEYSRTVVMQTYI 489

Query: 485 DELINPAGWRAWDKDFALNTSYYAEFGNFGPGSNTSERVNWLGFHLINDTDAGNFTAANF 544
           D  + P+GW AW  DFAL+T YYAE+ N GPGS+T+ RV W G+H+IN TDA NFT  NF
Sbjct: 490 DGFLEPSGWNAWSGDFALSTLYYAEYNNTGPGSDTTNRVTWPGYHVINATDASNFTVTNF 549

Query: 545 VLADDWLPQTGVPYTSGL 553
           ++ + W+ QTGVP+  GL
Sbjct: 550 LVGEGWIGQTGVPFVGGL 558

[Close]

BLAST of CmaCh08G004420 vs. ExPASy Swiss-Prot
Match: Q94CB1 (Probable pectinesterase/pectinesterase inhibitor 25 OS=Arabidopsis thaliana OX=3702 GN=PME25 PE=2 SV=1)

HSP 1 Score: 494.6 bits (1272), Expect = 1.5e-138
Identity = 264/544 (48.53%), Postives = 351/544 (64.52%), Query Frame = 0


Query: 24  SAASVCTSTPDPSYCK---SALPNQTGNVYSYGRSSFRKSLSSSQKFLRLVKKYLR---- 83
           S +  C STP P  C+   +A+ +   + Y YG+ + ++ L  + +  +++  Y R    
Sbjct: 78  SPSLACKSTPYPKLCRTILNAVKSSPSDPYRYGKFTIKQCLKQASRLSKVITSYARRVES 137

Query: 84  SRSSLTVPAVGALEDCKLLAGLNIDYLTTSFRAVNTTSSKLPEMKADDVQSLLSAILTNQ 143
              S T   +GA+ DC  L+ L+++YL T    +  T+  +     + V SLLS ++TNQ
Sbjct: 138 KPGSATAEEIGAVADCGELSELSVNYLETVTTELK-TAQVMTAALVEHVNSLLSGVVTNQ 197

Query: 144 QTCLDGLKATAGSWSVKNGLSLPLASDTKLYSMSLALFTKGWVPKKKKRSTWTAAGSLGG 203
           QTCLDGL      ++    +  P+ + T+LYS+SL L +   + +  KR   +    LGG
Sbjct: 198 QTCLDGLVEAKSGFAA--AIGSPMGNLTRLYSISLGLVSHA-LNRNLKRFKASKGKILGG 257

Query: 204 FRNGRMSLKMSNRAKAIYEMA--------TRRNLLETDDGGDDQISIRDIVIVSQDGSGN 263
             N      +    K + +          T RNL E  +     I +   VIV    S N
Sbjct: 258 -GNSTYREPLETLIKGLRKTCDNDKDCRKTSRNLGELGETSGGSILVSKAVIVGPFKSDN 317

Query: 264 FTTINEAIAAAPNNSAPTDGYFLIFVSVGVYEEYVSIAKNKRYLMMIGDGINQTVITGNR 323
           FTTI +AIAAAPNN+ P DGYF+I+   GVYEEY+ +  NK+ LM++GDGIN+T+ITGN 
Sbjct: 318 FTTITDAIAAAPNNTRPEDGYFVIYAREGVYEEYIVVPINKKNLMLMGDGINKTIITGNH 377

Query: 324 SVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVAVRNGADLSTFYLCSFEGYQ 383
           +VVDGWTT+N ++FAVVG  F+AV++TFRNTAG  KHQAVA+RN A+ S+FY CSFEGYQ
Sbjct: 378 NVVDGWTTYNCSSFAVVGERFMAVDVTFRNTAGPEKHQAVALRNNAEGSSFYRCSFEGYQ 437

Query: 384 DTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNIYPRLPMANQFNAITAQGRTDPNQ 443
           DTLY HSLRQFYR+CDIYGTVDFIFGNAA +FQNCNIY R PMA Q NAITA GR DPNQ
Sbjct: 438 DTLYVHSLRQFYRECDIYGTVDFIFGNAAAIFQNCNIYARKPMAKQKNAITAHGRLDPNQ 497

Query: 444 NTGTSIHNCRITAADDLANNTGVAVKTFLGRPWKEYSRTVYLQSFMDELINPAGWRAWDK 503
           NTG SI NC I AA DLA     A+ TFLGRPWK YSRTV++QS++ +++ P GW  W+ 
Sbjct: 498 NTGISIINCTIKAAPDLAAEPKSAM-TFLGRPWKPYSRTVFMQSYISDIVQPVGWLEWNG 557

Query: 504 DFALNTSYYAEFGNFGPGSNTSERVNWLGFHLINDTDAGNFTAANFVLADDWLPQTGVPY 553
              L+T YY E+ NFGPG+NT++RV WLG++L+N  +A NFT  NF + D WLPQT +P+
Sbjct: 558 TIGLDTIYYGEYSNFGPGANTNQRVQWLGYNLLNLAEAMNFTVYNFTMGDTWLPQTDIPF 615

[Close]

BLAST of CmaCh08G004420 vs. ExPASy Swiss-Prot
Match: Q9FF77 (Probable pectinesterase/pectinesterase inhibitor 47 OS=Arabidopsis thaliana OX=3702 GN=PME47 PE=2 SV=1)

HSP 1 Score: 485.7 bits (1249), Expect = 6.9e-136
Identity = 261/546 (47.80%), Postives = 346/546 (63.37%), Query Frame = 0


Query: 22  PLSAASVCTSTPDPSYCK---SALPNQTGNVYSYGRSSFRKSLSSSQKFLRLVKKYLR-- 81
           PL     C STP P  C+   SA+ +   + Y YG+ + ++ L  +++  +++ ++ +  
Sbjct: 81  PLPPNIACKSTPYPKLCRTILSAVKSSPSDPYHYGKFTMKQCLKQARRLSKVINRFAQRV 140

Query: 82  --SRSSLTVPAVGALEDCKLLAGLNIDYLTTSFRAVNTTSSKLPEMKADDVQSLLSAILT 141
                + TV  V A+ DC  LA L+++YL T    +   +  +     D V SLL  ++T
Sbjct: 141 EADPGTSTVEEVSAVADCGELAELSVEYLETVTEELK-AAELMTAALVDRVTSLLGGVVT 200

Query: 142 NQQTCLDGLKATAGSWSVKNGLSLPLASDTKLYSMSLALFTKGWVPKKKKRSTWTAAGSL 201
           NQQTCLDGL      ++    +  PL + T+LYS+SL L +       K+     + G +
Sbjct: 201 NQQTCLDGLVDAKSGFA--TAIGTPLGNLTRLYSVSLGLVSHALNRNLKRYK--GSKGKI 260

Query: 202 GGFRNGRMSLKMSNRAKAIYEMATR--------RNLLETDDGGDDQISIRDIVIVSQDGS 261
            G  N  +   +    K + +   +        RNL E  +     I +R+ V V    +
Sbjct: 261 FGGGNKPVREPLETLIKVLRKTCDKGKDCRKANRNLGELGETSGGSILVREAVTVGPYET 320

Query: 262 GNFTTINEAIAAAPNNSAPTDGYFLIFVSVGVYEEYVSIAKNKRYLMMIGDGINQTVITG 321
            NF TI EA+AAAPN++ P  GYF+I+   G+YEEYV I+  KR +M+IGDGIN+T+I+G
Sbjct: 321 DNFPTITEAVAAAPNHTFPEQGYFVIYARAGLYEEYVVISNKKRNIMLIGDGINKTIISG 380

Query: 322 NRSVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVAVRNGADLSTFYLCSFEG 381
           N S +DGWTT+NS+TFAVVG  FVAV++TFRNTAG  KHQAVAVRN AD STFY CSFEG
Sbjct: 381 NHSFIDGWTTYNSSTFAVVGDRFVAVDVTFRNTAGPEKHQAVAVRNNADGSTFYRCSFEG 440

Query: 382 YQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNIYPRLPMANQFNAITAQGRTDP 441
           YQDTLY HSLRQFYR+CDIYGT+DFIFGNAA +FQNCNIY R PMANQ NA+TA GRTDP
Sbjct: 441 YQDTLYVHSLRQFYRECDIYGTIDFIFGNAAAIFQNCNIYARKPMANQKNAVTAHGRTDP 500

Query: 442 NQNTGTSIHNCRITAADDLANNTGVAVKTFLGRPWKEYSRTVYLQSFMDELINPAGWRAW 501
           NQ TG SI NC I AA DLA +   +  TFLGRPWK YSRTVY+QS++ +++ P GW  W
Sbjct: 501 NQKTGISIINCTIGAAPDLAADP-KSTMTFLGRPWKPYSRTVYIQSYISDVVQPVGWLEW 560

Query: 502 DKDFALNTSYYAEFGNFGPGSNTSERVNWLGFHLINDTDAGNFTAANFVLADDWLPQTGV 553
           +    L+T  Y E+ NFGPG++TS+RV W G+ L+N   A NFT  NF L D WLPQT +
Sbjct: 561 NGTTGLDTISYGEYDNFGPGADTSKRVQWSGYSLLNLVQAMNFTVYNFTLGDTWLPQTDI 620

[Close]

BLAST of CmaCh08G004420 vs. TAIR 10
Match: AT1G02810.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 697.6 bits (1799), Expect = 8.2e-201
Identity = 353/571 (61.82%), Postives = 429/571 (75.13%), Query Frame = 0


Query: 9   LILLSLY----ITTAQIPLSAASVCTSTPDPSYCKSALPNQTGNVYSYGRSSFRKSLSSS 68
           LI LS +    + ++Q   +++++C +TPDP YCKS  P+  GNV  YG  S RKSLS S
Sbjct: 8   LITLSFFLQSVLASSQTLSNSSTICKTTPDPKYCKSVFPHSQGNVQQYGCFSIRKSLSQS 67

Query: 69  QKFLRLVKKYLRSRSSLTVPAV-GALEDCKLLAGLNIDYLTTSFRAVNTTSSK-----LP 128
           +KF+R V +Y++  + L+ PAV  AL+DC+ LAGL +DYL TSF  VN TS+K     L 
Sbjct: 68  RKFIRTVDRYIKRNAHLSQPAVIRALQDCRFLAGLTMDYLLTSFETVNDTSAKTSFKPLS 127

Query: 129 EMKADDVQSLLSAILTNQQTCLDGLKATA---GSWSVKNGLSLPLASDTKLYSMSLALFT 188
             KADD+Q+LLSA LTN+QTCL+GL   A    +W+V+ G++LPL +DTKL  +SLALFT
Sbjct: 128 FPKADDIQTLLSAALTNEQTCLEGLTTAASYSATWTVRTGVALPLVNDTKLLGVSLALFT 187

Query: 189 KGWVPKKKKRSTWTAAGSLGG----------FRNGRMSLKMSNRAKAIYEMATRRNLLET 248
           KGWVPKKKKR+ +  A    G          FRNG + LKM+ + KA+YE  +RR L + 
Sbjct: 188 KGWVPKKKKRAGFAWAQPRSGSSTHTKPFRLFRNGALPLKMTEKTKAVYESLSRRKLADG 247

Query: 249 DDGGD----DQISIRDIVIVSQDGSGNFTTINEAIAAAPNNSAPTDGYFLIFVSVGVYEE 308
           D  GD      + I DIV VSQDG+GNFT I  A+AAAPNN+  + G+FLI+V+ G+YEE
Sbjct: 248 DSNGDGDDGSMVLISDIVTVSQDGTGNFTNITAAVAAAPNNTDGSAGFFLIYVTAGIYEE 307

Query: 309 YVSIAKNKRYLMMIGDGINQTVITGNRSVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAG 368
           Y+SIAKNKRY+MMIGDGINQTV+TGNRSVVDGWTTFNSATFAV  P FVAVN+TFRNTAG
Sbjct: 308 YISIAKNKRYMMMIGDGINQTVVTGNRSVVDGWTTFNSATFAVTAPNFVAVNITFRNTAG 367

Query: 369 AIKHQAVAVRNGADLSTFYLCSFEGYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQ 428
             KHQAVA+R+GAD S FY CSFE YQDTLYTHSLRQFYR+CD+YGTVDFIFGNAAVVFQ
Sbjct: 368 PEKHQAVALRSGADFSIFYSCSFEAYQDTLYTHSLRQFYRECDVYGTVDFIFGNAAVVFQ 427

Query: 429 NCNIYPRLPMANQFNAITAQGRTDPNQNTGTSIHNCRITAADDLANNTGVAVKTFLGRPW 488
           NCN+YPR PM NQFNAITAQGR+DPNQNTGTSI NC I  ADDL  ++   VKT+LGRPW
Sbjct: 428 NCNLYPRKPMPNQFNAITAQGRSDPNQNTGTSIQNCTIKPADDLV-SSNYTVKTYLGRPW 487

Query: 489 KEYSRTVYLQSFMDELINPAGWRAWDKDFALNTSYYAEFGNFGPGSNTSERVNWLGFHLI 548
           KEYSRTVY+QS++D  + P GWR W+ DFAL+T YYAE+ N GPGSNT+ RV W G+H+I
Sbjct: 488 KEYSRTVYMQSYIDGFVEPVGWREWNGDFALSTLYYAEYNNTGPGSNTTNRVTWPGYHVI 547

Query: 549 NDTDAGNFTAANFVLADDWLPQTGVPYTSGL 553
           N TDA NFT     +  DW+ +TGVPYTSGL
Sbjct: 548 NSTDAANFTVTGLFIEADWIWKTGVPYTSGL 577

[Close]

BLAST of CmaCh08G004420 vs. TAIR 10
Match: AT4G02330.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 680.6 bits (1755), Expect = 1.0e-195
Identity = 350/564 (62.06%), Postives = 431/564 (76.42%), Query Frame = 0


Query: 4   TTFSSLILLSLYITTAQIPLSAASVCTSTPDPSYCKSALPNQT-GNVYSYGRSSFRKSLS 63
           T F SL  L +   T     S++++C +TPDP +CKS  P  + G+V  YGR S RKSL+
Sbjct: 10  TLFLSLQTLFIASQTLLPSNSSSTICKTTPDPKFCKSVFPQTSQGDVREYGRFSLRKSLT 69

Query: 64  SSQKFLRLVKKYL-RSRSSLTVPAVGALEDCKLLAGLNIDYLTTSFRAVN-TTSSK-LPE 123
            S+KF R + +YL R+ + L+  AVGAL+DC+ LA L  DYL TSF  VN TTSSK L  
Sbjct: 70  QSRKFTRTIDRYLKRNNALLSQSAVGALQDCRYLASLTTDYLITSFETVNITTSSKTLSF 129

Query: 124 MKADDVQSLLSAILTNQQTCLDGLK-ATAGSWSVKNGLSLPLASDTKLYSMSLALFTKGW 183
            KAD++Q+LLSA LTN+QTCLDG+  A + SW+++NG++LPL +DTKL+S+SLALFTKGW
Sbjct: 130 SKADEIQTLLSAALTNEQTCLDGINTAASSSWTIRNGVALPLINDTKLFSVSLALFTKGW 189

Query: 184 VPKKKK----------RSTWTAAGSLGGFRNGRMSLKMSNRAKAIYEMATRRNLLETDDG 243
           VPKKKK          ++T +       FRNG + LKM+   +A+YE  +RR L + DD 
Sbjct: 190 VPKKKKQVASYSWAHPKNTHSHTKPFRHFRNGALPLKMTEHTRAVYESLSRRKLAD-DDN 249

Query: 244 GDDQISIRDIVIVSQDGSGNFTTINEAIAAAPNNSAPTDGYFLIFVSVGVYEEYVSIAKN 303
             + + + DIV V+Q+G+GNFTTI EA+ +APN +  T GYF+I+V+ GVYEE V IAKN
Sbjct: 250 DVNTVLVSDIVTVNQNGTGNFTTITEAVNSAPNKTDGTAGYFVIYVTSGVYEENVVIAKN 309

Query: 304 KRYLMMIGDGINQTVITGNRSVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAV 363
           KRYLMMIGDGIN+TV+TGNR+VVDGWTTFNSATFAV  P FVAVNMTFRNTAG  KHQAV
Sbjct: 310 KRYLMMIGDGINRTVVTGNRNVVDGWTTFNSATFAVTSPNFVAVNMTFRNTAGPEKHQAV 369

Query: 364 AVRNGADLSTFYLCSFEGYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNIYPR 423
           A+R+ ADLS FY CSFE YQDTLYTHSLRQFYR+CDIYGTVDFIFGNAAVVFQ+CN+YPR
Sbjct: 370 AMRSSADLSIFYSCSFEAYQDTLYTHSLRQFYRECDIYGTVDFIFGNAAVVFQDCNLYPR 429

Query: 424 LPMANQFNAITAQGRTDPNQNTGTSIHNCRITAADDLANNTGVAVKTFLGRPWKEYSRTV 483
            PM NQFNAITAQGRTDPNQNTG SIHNC I  ADDL  ++   VKT+LGRPWKEYSRTV
Sbjct: 430 QPMQNQFNAITAQGRTDPNQNTGISIHNCTIKPADDLV-SSNYTVKTYLGRPWKEYSRTV 489

Query: 484 YLQSFMDELINPAGWRAWDKDFALNTSYYAEFGNFGPGSNTSERVNWLGFHLINDTDAGN 543
           ++QS++DE++ P GWR W+ DFAL+T YYAE+ N G GS+T++RV W G+H+IN TDA N
Sbjct: 490 FMQSYIDEVVEPVGWREWNGDFALSTLYYAEYNNTGSGSSTTDRVVWPGYHVINSTDANN 549

Query: 544 FTAANFVLADDWLPQTGVPYTSGL 553
           FT  NF+L D W+ Q+GVPY SGL
Sbjct: 550 FTVENFLLGDGWMVQSGVPYISGL 571

[Close]

BLAST of CmaCh08G004420 vs. TAIR 10
Match: AT2G47550.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 646.7 bits (1667), Expect = 1.6e-185
Identity = 326/558 (58.42%), Postives = 413/558 (74.01%), Query Frame = 0


Query: 5   TFSSLILLSLYITTAQIP-----LSAASVCTSTPDPSYCKSALPNQTGNVYSYGRSSFRK 64
           T + L  L     +AQIP      S +++C   PDPSYC+S LPNQ G++YSYGR S R+
Sbjct: 10  TLACLSSLPSPFISAQIPAIGNATSPSNICRFAPDPSYCRSVLPNQPGDIYSYGRLSLRR 69

Query: 65  SLSSSQKFLRLVKKYLRSRSSLTVPA-VGALEDCKLLAGLNIDYLTTSFRAVNTTSSKLP 124
           SLS +++F+ ++   L  +  +   + VGALEDCK LA L +DYL +S +  ++T + L 
Sbjct: 70  SLSRARRFISMIDAELDRKGKVAAKSTVGALEDCKFLASLTMDYLLSSSQTADSTKT-LS 129

Query: 125 EMKADDVQSLLSAILTNQQTCLDGLKATAGSWSVKNGLSLPLASDTKLYSMSLALFTKGW 184
             +A+DV + LSA +TN+QTCL+GLK+TA     +NGLS  L +DTKLY +SLALF+KGW
Sbjct: 130 LSRAEDVHTFLSAAITNEQTCLEGLKSTAS----ENGLSGDLFNDTKLYGVSLALFSKGW 189

Query: 185 VPKKKK-RSTWTAAG---SLGGFRNGRMSLKMSNRAKAIYEMATRRNLLETDDGGDDQIS 244
           VP++++ R  W          GFRNG++ LKM+ RA+A+Y   TRR LL++D    D + 
Sbjct: 190 VPRRQRSRPIWQPQARFKKFFGFRNGKLPLKMTERARAVYNTVTRRKLLQSD---ADAVQ 249

Query: 245 IRDIVIVSQDGSGNFTTINEAIAAAPNNSAPTDGYFLIFVSVGVYEEYVSIAKNKRYLMM 304
           + DIV V Q+G+GNFTTIN AIAAAPN +  ++GYFLI+V+ G+YEEYV + KNKRY+MM
Sbjct: 250 VSDIVTVIQNGTGNFTTINAAIAAAPNKTDGSNGYFLIYVTAGLYEEYVEVPKNKRYVMM 309

Query: 305 IGDGINQTVITGNRSVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVAVRNGA 364
           IGDGINQTVITGNRSVVDGWTTFNSATF + GP F+ VN+T RNTAG  K QAVA+R+G 
Sbjct: 310 IGDGINQTVITGNRSVVDGWTTFNSATFILSGPNFIGVNITIRNTAGPTKGQAVALRSGG 369

Query: 365 DLSTFYLCSFEGYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNIYPRLPMANQ 424
           DLS FY CSFE YQDTLYTHSLRQFYR+CD+YGTVDFIFGNAAVV QNCN+YPR P   Q
Sbjct: 370 DLSVFYSCSFEAYQDTLYTHSLRQFYRECDVYGTVDFIFGNAAVVLQNCNLYPRQPRKGQ 429

Query: 425 FNAITAQGRTDPNQNTGTSIHNCRITAADDLANNTGVAVKTFLGRPWKEYSRTVYLQSFM 484
            N +TAQGRTDPNQNTGT+IH C I  ADDLA  +   VKT+LGRPWKEYSRTV +Q+++
Sbjct: 430 SNEVTAQGRTDPNQNTGTAIHGCTIRPADDLA-TSNYTVKTYLGRPWKEYSRTVVMQTYI 489

Query: 485 DELINPAGWRAWDKDFALNTSYYAEFGNFGPGSNTSERVNWLGFHLINDTDAGNFTAANF 544
           D  + P+GW AW  DFAL+T YYAE+ N GPGS+T+ RV W G+H+IN TDA NFT  NF
Sbjct: 490 DGFLEPSGWNAWSGDFALSTLYYAEYNNTGPGSDTTNRVTWPGYHVINATDASNFTVTNF 549

Query: 545 VLADDWLPQTGVPYTSGL 553
           ++ + W+ QTGVP+  GL
Sbjct: 550 LVGEGWIGQTGVPFVGGL 558

[Close]

BLAST of CmaCh08G004420 vs. TAIR 10
Match: AT3G10720.2 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 494.6 bits (1272), Expect = 1.0e-139
Identity = 264/544 (48.53%), Postives = 351/544 (64.52%), Query Frame = 0


Query: 24  SAASVCTSTPDPSYCK---SALPNQTGNVYSYGRSSFRKSLSSSQKFLRLVKKYLR---- 83
           S +  C STP P  C+   +A+ +   + Y YG+ + ++ L  + +  +++  Y R    
Sbjct: 78  SPSLACKSTPYPKLCRTILNAVKSSPSDPYRYGKFTIKQCLKQASRLSKVITSYARRVES 137

Query: 84  SRSSLTVPAVGALEDCKLLAGLNIDYLTTSFRAVNTTSSKLPEMKADDVQSLLSAILTNQ 143
              S T   +GA+ DC  L+ L+++YL T    +  T+  +     + V SLLS ++TNQ
Sbjct: 138 KPGSATAEEIGAVADCGELSELSVNYLETVTTELK-TAQVMTAALVEHVNSLLSGVVTNQ 197

Query: 144 QTCLDGLKATAGSWSVKNGLSLPLASDTKLYSMSLALFTKGWVPKKKKRSTWTAAGSLGG 203
           QTCLDGL      ++    +  P+ + T+LYS+SL L +   + +  KR   +    LGG
Sbjct: 198 QTCLDGLVEAKSGFAA--AIGSPMGNLTRLYSISLGLVSHA-LNRNLKRFKASKGKILGG 257

Query: 204 FRNGRMSLKMSNRAKAIYEMA--------TRRNLLETDDGGDDQISIRDIVIVSQDGSGN 263
             N      +    K + +          T RNL E  +     I +   VIV    S N
Sbjct: 258 -GNSTYREPLETLIKGLRKTCDNDKDCRKTSRNLGELGETSGGSILVSKAVIVGPFKSDN 317

Query: 264 FTTINEAIAAAPNNSAPTDGYFLIFVSVGVYEEYVSIAKNKRYLMMIGDGINQTVITGNR 323
           FTTI +AIAAAPNN+ P DGYF+I+   GVYEEY+ +  NK+ LM++GDGIN+T+ITGN 
Sbjct: 318 FTTITDAIAAAPNNTRPEDGYFVIYAREGVYEEYIVVPINKKNLMLMGDGINKTIITGNH 377

Query: 324 SVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVAVRNGADLSTFYLCSFEGYQ 383
           +VVDGWTT+N ++FAVVG  F+AV++TFRNTAG  KHQAVA+RN A+ S+FY CSFEGYQ
Sbjct: 378 NVVDGWTTYNCSSFAVVGERFMAVDVTFRNTAGPEKHQAVALRNNAEGSSFYRCSFEGYQ 437

Query: 384 DTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNIYPRLPMANQFNAITAQGRTDPNQ 443
           DTLY HSLRQFYR+CDIYGTVDFIFGNAA +FQNCNIY R PMA Q NAITA GR DPNQ
Sbjct: 438 DTLYVHSLRQFYRECDIYGTVDFIFGNAAAIFQNCNIYARKPMAKQKNAITAHGRLDPNQ 497

Query: 444 NTGTSIHNCRITAADDLANNTGVAVKTFLGRPWKEYSRTVYLQSFMDELINPAGWRAWDK 503
           NTG SI NC I AA DLA     A+ TFLGRPWK YSRTV++QS++ +++ P GW  W+ 
Sbjct: 498 NTGISIINCTIKAAPDLAAEPKSAM-TFLGRPWKPYSRTVFMQSYISDIVQPVGWLEWNG 557

Query: 504 DFALNTSYYAEFGNFGPGSNTSERVNWLGFHLINDTDAGNFTAANFVLADDWLPQTGVPY 553
              L+T YY E+ NFGPG+NT++RV WLG++L+N  +A NFT  NF + D WLPQT +P+
Sbjct: 558 TIGLDTIYYGEYSNFGPGANTNQRVQWLGYNLLNLAEAMNFTVYNFTMGDTWLPQTDIPF 615

[Close]

BLAST of CmaCh08G004420 vs. TAIR 10
Match: AT5G04970.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 485.7 bits (1249), Expect = 4.9e-137
Identity = 261/546 (47.80%), Postives = 346/546 (63.37%), Query Frame = 0


Query: 22  PLSAASVCTSTPDPSYCK---SALPNQTGNVYSYGRSSFRKSLSSSQKFLRLVKKYLR-- 81
           PL     C STP P  C+   SA+ +   + Y YG+ + ++ L  +++  +++ ++ +  
Sbjct: 81  PLPPNIACKSTPYPKLCRTILSAVKSSPSDPYHYGKFTMKQCLKQARRLSKVINRFAQRV 140

Query: 82  --SRSSLTVPAVGALEDCKLLAGLNIDYLTTSFRAVNTTSSKLPEMKADDVQSLLSAILT 141
                + TV  V A+ DC  LA L+++YL T    +   +  +     D V SLL  ++T
Sbjct: 141 EADPGTSTVEEVSAVADCGELAELSVEYLETVTEELK-AAELMTAALVDRVTSLLGGVVT 200

Query: 142 NQQTCLDGLKATAGSWSVKNGLSLPLASDTKLYSMSLALFTKGWVPKKKKRSTWTAAGSL 201
           NQQTCLDGL      ++    +  PL + T+LYS+SL L +       K+     + G +
Sbjct: 201 NQQTCLDGLVDAKSGFA--TAIGTPLGNLTRLYSVSLGLVSHALNRNLKRYK--GSKGKI 260

Query: 202 GGFRNGRMSLKMSNRAKAIYEMATR--------RNLLETDDGGDDQISIRDIVIVSQDGS 261
            G  N  +   +    K + +   +        RNL E  +     I +R+ V V    +
Sbjct: 261 FGGGNKPVREPLETLIKVLRKTCDKGKDCRKANRNLGELGETSGGSILVREAVTVGPYET 320

Query: 262 GNFTTINEAIAAAPNNSAPTDGYFLIFVSVGVYEEYVSIAKNKRYLMMIGDGINQTVITG 321
            NF TI EA+AAAPN++ P  GYF+I+   G+YEEYV I+  KR +M+IGDGIN+T+I+G
Sbjct: 321 DNFPTITEAVAAAPNHTFPEQGYFVIYARAGLYEEYVVISNKKRNIMLIGDGINKTIISG 380

Query: 322 NRSVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVAVRNGADLSTFYLCSFEG 381
           N S +DGWTT+NS+TFAVVG  FVAV++TFRNTAG  KHQAVAVRN AD STFY CSFEG
Sbjct: 381 NHSFIDGWTTYNSSTFAVVGDRFVAVDVTFRNTAGPEKHQAVAVRNNADGSTFYRCSFEG 440

Query: 382 YQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNIYPRLPMANQFNAITAQGRTDP 441
           YQDTLY HSLRQFYR+CDIYGT+DFIFGNAA +FQNCNIY R PMANQ NA+TA GRTDP
Sbjct: 441 YQDTLYVHSLRQFYRECDIYGTIDFIFGNAAAIFQNCNIYARKPMANQKNAVTAHGRTDP 500

Query: 442 NQNTGTSIHNCRITAADDLANNTGVAVKTFLGRPWKEYSRTVYLQSFMDELINPAGWRAW 501
           NQ TG SI NC I AA DLA +   +  TFLGRPWK YSRTVY+QS++ +++ P GW  W
Sbjct: 501 NQKTGISIINCTIGAAPDLAADP-KSTMTFLGRPWKPYSRTVYIQSYISDVVQPVGWLEW 560

Query: 502 DKDFALNTSYYAEFGNFGPGSNTSERVNWLGFHLINDTDAGNFTAANFVLADDWLPQTGV 553
           +    L+T  Y E+ NFGPG++TS+RV W G+ L+N   A NFT  NF L D WLPQT +
Sbjct: 561 NGTTGLDTISYGEYDNFGPGADTSKRVQWSGYSLLNLVQAMNFTVYNFTLGDTWLPQTDI 620

The following BLAST results are available for this feature:

BLAST of CmaCh08G004420 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SRX4	1.1e-199	61.82	Probable pectinesterase/pectinesterase inhibitor 7 OS=Arabidopsis thaliana OX=37...	[more]
Q8RXK7	1.5e-194	62.06	Probable pectinesterase/pectinesterase inhibitor 41 OS=Arabidopsis thaliana OX=3...	[more]
O22256	2.3e-184	58.42	Probable pectinesterase/pectinesterase inhibitor 20 OS=Arabidopsis thaliana OX=3...	[more]
Q94CB1	1.5e-138	48.53	Probable pectinesterase/pectinesterase inhibitor 25 OS=Arabidopsis thaliana OX=3...	[more]
Q9FF77	6.9e-136	47.80	Probable pectinesterase/pectinesterase inhibitor 47 OS=Arabidopsis thaliana OX=3...	[more]

BLAST of CmaCh08G004420 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G02810.1	8.2e-201	61.82	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT4G02330.1	1.0e-195	62.06	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT2G47550.1	1.6e-185	58.42	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT3G10720.2	1.0e-139	48.53	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT5G04970.1	4.9e-137	47.80	Plant invertase/pectin methylesterase inhibitor superfamily	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 552..554
None	No IPR available	PANTHER	PTHR31707:SF294	PECTINESTERASE/PECTINESTERASE INHIBITOR 7-RELATED	coord: 14..552
None	No IPR available	PANTHER	PTHR31707	PECTINESTERASE	coord: 14..552
None	No IPR available	CDD	cd15798	PMEI-like_3	coord: 27..178 e-value: 7.90143E-29 score: 109.835
IPR006501	Pectinesterase inhibitor domain	SMART	SM00856	PMEI_2	coord: 20..173 e-value: 6.1E-22 score: 88.9
IPR006501	Pectinesterase inhibitor domain	PFAM	PF04043	PMEI	coord: 26..173 e-value: 1.1E-17 score: 64.8
IPR000070	Pectinesterase, catalytic	PFAM	PF01095	Pectinesterase	coord: 239..537 e-value: 1.1E-133 score: 444.6
IPR012334	Pectin lyase fold	GENE3D	2.160.20.10		coord: 233..552 e-value: 6.1E-118 score: 395.8
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	GENE3D	1.20.140.40	Invertase/pectin methylesterase inhibitor family protein	coord: 24..176 e-value: 2.4E-21 score: 78.3
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	SUPERFAMILY	101148	Plant invertase/pectin methylesterase inhibitor	coord: 27..176
IPR033131	Pectinesterase, Asp active site	PROSITE	PS00503	PECTINESTERASE_2	coord: 385..394
IPR011050	Pectin lyase fold/virulence factor	SUPERFAMILY	51126	Pectin lyase-like	coord: 237..551

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmaCh08G004420.1	CmaCh08G004420.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0042545	cell wall modification
biological_process	GO:0043086	negative regulation of catalytic activity
biological_process	GO:0045490	pectin catabolic process
molecular_function	GO:0045330	aspartyl esterase activity
molecular_function	GO:0030599	pectinesterase activity
molecular_function	GO:0046910	pectinesterase inhibitor activity
molecular_function	GO:0004857	enzyme inhibitor activity