CmaCh08G003450 (gene) Cucurbita maxima (Rimu) v1.1

Overview


Name	CmaCh08G003450
Type	gene
Organism	Cucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Description	Pre-mRNA-splicing factor 18
Location	Cma_Chr08: 1975011 .. 1978772 (-)
RNA-Seq Expression	CmaCh08G003450
Synteny	CmaCh08G003450

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CGTTCTGAATTTCAATAGAGGTTTCCCTTTCCTTTCTCCTCCCTCTTTGAAATCAGTTCTGAAAGTAAGCCCTAACAGGATTCCGGGGAGGAGAACCAGAGCAGAACCAATGGATCTCCTGAAGCAAGAACTTCTCAAGAAACGTCAATCCCTAGCTACCGCAACCGGTGGCAAACGCTTCTTCAAGCGATCCGAGATTGAACAGAAGCAAATCCAGCAGCTACGCGAGAAAGAGAAGCGCGAATTGGAAACCAAAGCTCTCCGCCAATCCACCAGCTCAGCCGCCACCGCCGAGGCCAACTCCACATCTTCCAAATCCAATTCATTAGCTTCCGCCTCCACTACTTCCTCTACCACTATTAACTCCGCCGCCACCACTTCGAAATCGCTTACGGACGAGCAGAACATCGACAACCTCGTCCTTCCTAGGCAGGAAGTAATCAGAAGGCTCCGGTTTCTGAAGCAGCCCATTACGCTATTCGGGGAAGATGATGATACTCGGCTCGACCGTTTGAAGTATGTGCTGAAAGCGGGACTTTTCGAGGTGGATAGTGATTTGACGGAGGGGCAAACGAACGATTTTCTTCGGGATATTGCAGAGTTGAGGAAGCGGCAGAAGACGGGTATTTTGAGTGAGAGGAAGAGGCAGAAGAAGGAAGACGGCGCTGGAGAAGAGGGCGAAGGGGGTGGGGCTGACGAAGAAAGTGCCGATGGAGGGTCGAGTGGGGTCGACGCTGATAAAGATTTGAAGAGAATGAAAGCGAATTTTGATGAATTATGTCAAGAAGACAAGATACTAGTATTCTTTAAGAGGTTGTTGAATGAGTGGAACCAGGAATTGGATGAAATGCCCGAAGCAGAGAAGCGGACGGCGAAAGGGAAATCTATGGTAGCGACGTTCAAGCAGTGTGCTCGCTATTTGCATCCTCTGTTCAAGTTTTGTAGGAAGAAGGTACAATTTTGTCCCTATATACTTATTCAATTTGGTGAATGTTCCATTTTCCCTTGATTCTGCTTTATGTTATCTTTTGACTTCATGGTAGATTAGAGAATAGCTAGAGCTTCTTTTTCTGTCATTCCTTTCGCTCTGCAAGTGCTCTGATGGAAAAATGGGAAAATGTTGAGGATTATTGGGAGAGGAGTCAACATCGGCTAATTAAGGGGTTGATCATGAGTTTATAAGTAAGAAACACTATCTTCATTGGTATGAAGACTTTTGAGGAAACCAAAAGCAAAACCTATGGAGATCCGTGGTTCCAAACATGGTATCAGAGCCATGTAAATAAAATCCTAAAATGTTAGATCAAAGAAGTTGTGAGTCTCGAAAGTGTAGTCAAAAGTGCTCAAGTGTCGAACAATGTACTTTGTTCGAAGGCTCCAGAACCGGAGTTGAGCCTCGATTAAGGGGAGGTTCTATGGTGTACTTTGTTCAAGGGAAGGATTGTTGAGGATTGTTGAGGATTGTTGGGAGAGGAGTCCCACATCGGCTAATTAAGGGGTTGATCATGAGTTTACAAGTATGGAACACTATCTTCATTGGTATGAGGTATTTTGGGGAAACCAAAAGCAAAACTACGAGAGTTTATGCTCAAAGTGGACAATATGATATCATTGTAGAGGTGGATGTTTTTTAAGAGAAAAAAGTTCCCACATGAGCTTGTAGATTTCCTCTTTAGGTTGATTATCATAGGAAGCAATCTCTAACAAATTACATCCTACGGTTTTCCTCTTTAACTATGATTATCAATCAAGCAGCGAAGAGAGTAGGCTGCTAACTTAAGGGGGAGGACCACTTGCCACTTGAATCTCATGCGCATGTTATTCTGCAGTTCCTCTGCCAATTGGCGCTTGAGTAAGAGATGTCTTTGGGATTCAACGTCTTTCATGCGGAGAAAGCGAGAGGGACCAATTTGGAGGCCCTTTGTTTTATGTCGTGATAATCTTATGAAAGAGCTCCCATTGGAAAAGCTCAACTTGTGAGATCCCACAATAGTTGGAGAGAACGAAACATTACTTATAAGGGTGTGGAAATCTCTTCCTAGAAGACGCGTTTTAAAACCTTGGGGGGAAGCCAAGAAGGGAAAGCCCAAAGAGGACAATGCTAGCTGTGAACTTGAATTGTTACTAATGGTATAAGAGCCAGACACCAGGCGGTATACCAGCGAGGACGCTGGGCCCCAAGAGGGGTGGATTGTGAGATCTCACATTGGTTGGAGAGAAGAACAAAACATTTCTTATAAGAGTGTGGAAACCTCTCCCTAGAAGACACGTTTTAAAATCTTGAGGGGAAGCCAAGAAAGGAAAACCCAAAGAGAACAATATCTACTAGCGGTGGGGTGGGCTTGGGCTTGGGCTGTTACACGACTTTCTTTGAACATATATGAGTGACATATCATCTTGTAAAGATTTGGGTTCTTTGAGAATCCTTTGTTCAAAATATCCTTGAATAGGTAGTTGCATGAACGGACCTCCTGTTAGTCAGACTGCCAAATTCATTTATTTTGGAAGAACAGTAAGTAGAACTTAGAAGTGGGAGATCAAGTTTAGGAAAGAGGCCCGTTTTGTGATTTCATCATCCAGATAAGAAAATCTAGAATCAGGATCCCAATTTCGAGTAGAGGAGTTCCAAATATCTTGGATAGAAGTGGTTCAATCTTCTTGGGAAAGCGCATTCCAAAGCTTGTAGAACTGCCATTCCAATGGAGGTTTTGACCCTCTAGTGGAGTTAAGTTTTCTTAATTGATTTTAGGTCATCGATGGCCTCTCGACGCGTGTAGAGAAGGAGGTACTAACAAAAAGTCACCAAATTTAGCCACCATAACTTTCCTTCATAAAACTTATATTTTTATTATAGAATCTCAAGTAGATCTATATTTTGTTAATGCCAAGTATCATGTTAGATTTTAATATTCTTCTTTTAGATTCTTCCTGACGATATTCGGCAAGCATTGTTGGTGGTCGTTGAATGTTGCATGGAGCGAGACTATCTAGCTGCAATGGATCAATACATTAAGATGGCCATTGGAAATGCCCCCTGGCCTATTGGTGTCACTATGGTTGGTATCCACGAACGTTCAGCTCGTGAGAAGATTTACACCAACAGTGTAGCCCATATCATGAACGATGAGACGACAAGGAAATACTTGCAATCGATCAAGAGATTGATGACGTTCTGTCAACGACGCTATCCAACAATGCCATCCAAGGCAGTAGAATTTAACAGCTTGGCGAACGGTAGCGACCTGCAGTCCCTGCTAGCAGAAGAGAGAGTCTCTGGAGGATGGGGCAAGCAGGGTTCTGATGAAAGGCTTCGCATCATGCACTCCCCGGAAGATAGCTAGCTAGCAAGTAAGAATATACTCTCCTGGCTGCTACTCATTACTCTTCTGCATATTTTGGTAGTCAGTCAGCAACTTCTGAGTACCGTTTGATATCAAAGTCTATAGTATTCGTTCAAGATAGCTACCTGGTAAGAAGATTATCTCTTGGTTACCATTGTTTATGTCTCTTTGTTTGTAATTTGAAGTTTCATCAGCTTAGTTTGGTAGTCAGTAAGTCAATGTCTGGGCATTGATTATGAAGAGACTTAAGGCTCAATCGTTAGAACATATCGTTGGAAACGACGACCTTTTATATTGGGCTTCTCTAGAGCCCTTGAACTTCTCCTTAATTAAGGCTTGACATCTTCTAAGCTTAAGGTCCAATGGTTAGTTCATACGGTTAGAAATGACCCTCCACGTTGGGCTTCTCTAGAGCCCTTGA

mRNA sequence

CGTTCTGAATTTCAATAGAGGTTTCCCTTTCCTTTCTCCTCCCTCTTTGAAATCAGTTCTGAAAGTAAGCCCTAACAGGATTCCGGGGAGGAGAACCAGAGCAGAACCAATGGATCTCCTGAAGCAAGAACTTCTCAAGAAACGTCAATCCCTAGCTACCGCAACCGGTGGCAAACGCTTCTTCAAGCGATCCGAGATTGAACAGAAGCAAATCCAGCAGCTACGCGAGAAAGAGAAGCGCGAATTGGAAACCAAAGCTCTCCGCCAATCCACCAGCTCAGCCGCCACCGCCGAGGCCAACTCCACATCTTCCAAATCCAATTCATTAGCTTCCGCCTCCACTACTTCCTCTACCACTATTAACTCCGCCGCCACCACTTCGAAATCGCTTACGGACGAGCAGAACATCGACAACCTCGTCCTTCCTAGGCAGGAAGTAATCAGAAGGCTCCGGTTTCTGAAGCAGCCCATTACGCTATTCGGGGAAGATGATGATACTCGGCTCGACCGTTTGAAGTATGTGCTGAAAGCGGGACTTTTCGAGGTGGATAGTGATTTGACGGAGGGGCAAACGAACGATTTTCTTCGGGATATTGCAGAGTTGAGGAAGCGGCAGAAGACGGGTATTTTGAGTGAGAGGAAGAGGCAGAAGAAGGAAGACGGCGCTGGAGAAGAGGGCGAAGGGGGTGGGGCTGACGAAGAAAGTGCCGATGGAGGGTCGAGTGGGGTCGACGCTGATAAAGATTTGAAGAGAATGAAAGCGAATTTTGATGAATTATGTCAAGAAGACAAGATACTAGTATTCTTTAAGAGGTTGTTGAATGAGTGGAACCAGGAATTGGATGAAATGCCCGAAGCAGAGAAGCGGACGGCGAAAGGGAAATCTATGGTAGCGACGTTCAAGCAGTGTGCTCGCTATTTGCATCCTCTGTTCAAGTTTTGTAGGAAGAAGATTCTTCCTGACGATATTCGGCAAGCATTGTTGGTGGTCGTTGAATGTTGCATGGAGCGAGACTATCTAGCTGCAATGGATCAATACATTAAGATGGCCATTGGAAATGCCCCCTGGCCTATTGGTGTCACTATGGTTGGTATCCACGAACGTTCAGCTCGTGAGAAGATTTACACCAACAGTGTAGCCCATATCATGAACGATGAGACGACAAGGAAATACTTGCAATCGATCAAGAGATTGATGACGTTCTGTCAACGACGCTATCCAACAATGCCATCCAAGGCAGTAGAATTTAACAGCTTGGCGAACGGTAGCGACCTGCAGTCCCTGCTAGCAGAAGAGAGAGTCTCTGGAGGATGGGGCAAGCAGGGTTCTGATGAAAGGCTTCGCATCATGCACTCCCCGGAAGATAGCTAGCTAGCAAGTAAGAATATACTCTCCTGGCTGCTACTCATTACTCTTCTGCATATTTTGGTAGTCAGTCAGCAACTTCTGAGTACCGTTTGATATCAAAGTCTATAGTATTCGTTCAAGATAGCTACCTGGTAAGAAGATTATCTCTTGGTTACCATTGTTTATGTCTCTTTGTTTGTAATTTGAAGTTTCATCAGCTTAGTTTGGTAGTCAGTAAGTCAATGTCTGGGCATTGATTATGAAGAGACTTAAGGCTCAATCGTTAGAACATATCGTTGGAAACGACGACCTTTTATATTGGGCTTCTCTAGAGCCCTTGAACTTCTCCTTAATTAAGGCTTGACATCTTCTAAGCTTAAGGTCCAATGGTTAGTTCATACGGTTAGAAATGACCCTCCACGTTGGGCTTCTCTAGAGCCCTTGA

Coding sequence (CDS)

ATGGATCTCCTGAAGCAAGAACTTCTCAAGAAACGTCAATCCCTAGCTACCGCAACCGGTGGCAAACGCTTCTTCAAGCGATCCGAGATTGAACAGAAGCAAATCCAGCAGCTACGCGAGAAAGAGAAGCGCGAATTGGAAACCAAAGCTCTCCGCCAATCCACCAGCTCAGCCGCCACCGCCGAGGCCAACTCCACATCTTCCAAATCCAATTCATTAGCTTCCGCCTCCACTACTTCCTCTACCACTATTAACTCCGCCGCCACCACTTCGAAATCGCTTACGGACGAGCAGAACATCGACAACCTCGTCCTTCCTAGGCAGGAAGTAATCAGAAGGCTCCGGTTTCTGAAGCAGCCCATTACGCTATTCGGGGAAGATGATGATACTCGGCTCGACCGTTTGAAGTATGTGCTGAAAGCGGGACTTTTCGAGGTGGATAGTGATTTGACGGAGGGGCAAACGAACGATTTTCTTCGGGATATTGCAGAGTTGAGGAAGCGGCAGAAGACGGGTATTTTGAGTGAGAGGAAGAGGCAGAAGAAGGAAGACGGCGCTGGAGAAGAGGGCGAAGGGGGTGGGGCTGACGAAGAAAGTGCCGATGGAGGGTCGAGTGGGGTCGACGCTGATAAAGATTTGAAGAGAATGAAAGCGAATTTTGATGAATTATGTCAAGAAGACAAGATACTAGTATTCTTTAAGAGGTTGTTGAATGAGTGGAACCAGGAATTGGATGAAATGCCCGAAGCAGAGAAGCGGACGGCGAAAGGGAAATCTATGGTAGCGACGTTCAAGCAGTGTGCTCGCTATTTGCATCCTCTGTTCAAGTTTTGTAGGAAGAAGATTCTTCCTGACGATATTCGGCAAGCATTGTTGGTGGTCGTTGAATGTTGCATGGAGCGAGACTATCTAGCTGCAATGGATCAATACATTAAGATGGCCATTGGAAATGCCCCCTGGCCTATTGGTGTCACTATGGTTGGTATCCACGAACGTTCAGCTCGTGAGAAGATTTACACCAACAGTGTAGCCCATATCATGAACGATGAGACGACAAGGAAATACTTGCAATCGATCAAGAGATTGATGACGTTCTGTCAACGACGCTATCCAACAATGCCATCCAAGGCAGTAGAATTTAACAGCTTGGCGAACGGTAGCGACCTGCAGTCCCTGCTAGCAGAAGAGAGAGTCTCTGGAGGATGGGGCAAGCAGGGTTCTGATGAAAGGCTTCGCATCATGCACTCCCCGGAAGATAGCTAG

Protein sequence

MDLLKQELLKKRQSLATATGGKRFFKRSEIEQKQIQQLREKEKRELETKALRQSTSSAATAEANSTSSKSNSLASASTTSSTTINSAATTSKSLTDEQNIDNLVLPRQEVIRRLRFLKQPITLFGEDDDTRLDRLKYVLKAGLFEVDSDLTEGQTNDFLRDIAELRKRQKTGILSERKRQKKEDGAGEEGEGGGADEESADGGSSGVDADKDLKRMKANFDELCQEDKILVFFKRLLNEWNQELDEMPEAEKRTAKGKSMVATFKQCARYLHPLFKFCRKKILPDDIRQALLVVVECCMERDYLAAMDQYIKMAIGNAPWPIGVTMVGIHERSAREKIYTNSVAHIMNDETTRKYLQSIKRLMTFCQRRYPTMPSKAVEFNSLANGSDLQSLLAEERVSGGWGKQGSDERLRIMHSPEDS

Homology

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BLAST of CmaCh08G003450 vs. ExPASy Swiss-Prot
Match: Q99633 (Pre-mRNA-splicing factor 18 OS=Homo sapiens OX=9606 GN=PRPF18 PE=1 SV=1)

HSP 1 Score: 186.4 bits (472), Expect = 6.5e-46
Identity = 135/386 (34.97%), Postives = 204/386 (52.85%), Query Frame = 0


Query: 1   MDLLKQELLKKRQSLA---TATGGKRFFKRSEIEQKQIQQLREKEKRELETKALRQSTSS 60
           MD+LK E+L+KRQ +         K++FKRSE+ +K+ +   E+   +++ K   Q    
Sbjct: 1   MDILKSEILRKRQLVEDRNLLVENKKYFKRSELAKKEEEAYFERCGYKIQPKEEDQK--- 60

Query: 61  AATAEANSTSSKSNSLASASTTSSTTINSAATTSKSLTDEQNIDNLVLPRQEVIRRLRFL 120
                                TSS  +       + L        + L RQEVIRRLR  
Sbjct: 61  -------------------PLTSSNPVLELELAEEKLP-------MTLSRQEVIRRLRER 120

Query: 121 KQPITLFGEDDDTRLDRLKYVLKAGLFEVDSDLTEGQTNDFLRDIAELRKRQKTGILSER 180
            +PI LFGE D     RL+ +       +  ++ +G  ND    + ++ ++    I+   
Sbjct: 121 GEPIRLFGETDYDAFQRLRKI-----EILTPEVNKGLRNDLKAALDKIDQQYLNEIV--- 180

Query: 181 KRQKKEDGAGEEGEGGGADEESADGGSSGVDADKDLKRMKANFDELCQEDKILVFFKRLL 240
                  G  E GE    ++      ++ ++  + L       D+    D I  F K LL
Sbjct: 181 -------GGQEPGEEDTQNDLKVHEENTTIEELEALGESLGKGDDHKDMDIITKFLKFLL 240

Query: 241 NEWNQELDEMPEAEKRTAKGKSMVATFKQCARYLHPLFKFCRKKILPDDIRQALLVVVEC 300
             W +EL+   +  KR+ +GK   AT KQ   YL PLF+  RK+ LP DI++++  +++ 
Sbjct: 241 GVWAKELNAREDYVKRSVQGKLNSATQKQTESYLRPLFRKLRKRNLPADIKESITDIIKF 300

Query: 301 CMERDYLAAMDQYIKMAIGNAPWPIGVTMVGIHERSAREKIYTNSVAHIMNDETTRKYLQ 360
            ++R+Y+ A D Y++MAIGNAPWPIGVTMVGIH R+ REKI++  VAH++NDET RKY+Q
Sbjct: 301 MLQREYVKANDAYLQMAIGNAPWPIGVTMVGIHARTGREKIFSKHVAHVLNDETQRKYIQ 342

Query: 361 SIKRLMTFCQRRYPTMPSKAVEFNSL 384
            +KRLMT CQ+ +PT PSK VE+N+L
Sbjct: 361 GLKRLMTICQKHFPTDPSKCVEYNAL 342

[Close]

BLAST of CmaCh08G003450 vs. ExPASy Swiss-Prot
Match: Q8BM39 (Pre-mRNA-splicing factor 18 OS=Mus musculus OX=10090 GN=Prpf18 PE=1 SV=1)

HSP 1 Score: 186.4 bits (472), Expect = 6.5e-46
Identity = 135/386 (34.97%), Postives = 204/386 (52.85%), Query Frame = 0


Query: 1   MDLLKQELLKKRQSLA---TATGGKRFFKRSEIEQKQIQQLREKEKRELETKALRQSTSS 60
           MD+LK E+L+KRQ +         K++FKRSE+ +K+ +   E+   +++ K   Q    
Sbjct: 1   MDILKSEILRKRQLVEDRNLLVENKKYFKRSELARKEEEAYYERCGYKIQPKEDDQK--- 60

Query: 61  AATAEANSTSSKSNSLASASTTSSTTINSAATTSKSLTDEQNIDNLVLPRQEVIRRLRFL 120
                                TSS  +       + L        + L RQEVIRRLR  
Sbjct: 61  -------------------PLTSSNPVLELELAEEKLP-------MTLSRQEVIRRLRER 120

Query: 121 KQPITLFGEDDDTRLDRLKYVLKAGLFEVDSDLTEGQTNDFLRDIAELRKRQKTGILSER 180
            +PI LFGE D     RL+ +       +  ++ +G  ND    + ++ ++    I+   
Sbjct: 121 GEPIRLFGETDYDAFQRLRKI-----EILTPEVNKGLRNDLKAALDKIDQQYLNEIV--- 180

Query: 181 KRQKKEDGAGEEGEGGGADEESADGGSSGVDADKDLKRMKANFDELCQEDKILVFFKRLL 240
                  G  E GE    ++      ++ ++  + L       D+    D I  F K LL
Sbjct: 181 -------GGQEPGEEDTQNDLKVHEENTTIEELEALGESLGKGDDHKDMDIITKFLKFLL 240

Query: 241 NEWNQELDEMPEAEKRTAKGKSMVATFKQCARYLHPLFKFCRKKILPDDIRQALLVVVEC 300
             W +EL+   +  KR+ +GK   AT KQ   YL PLF+  RK+ LP DI++++  +++ 
Sbjct: 241 GVWAKELNAREDYVKRSVQGKLNSATQKQTESYLRPLFRKLRKRNLPADIKESITDIIKF 300

Query: 301 CMERDYLAAMDQYIKMAIGNAPWPIGVTMVGIHERSAREKIYTNSVAHIMNDETTRKYLQ 360
            ++R+Y+ A D Y++MAIGNAPWPIGVTMVGIH R+ REKI++  VAH++NDET RKY+Q
Sbjct: 301 MLQREYVKANDAYLQMAIGNAPWPIGVTMVGIHARTGREKIFSKHVAHVLNDETQRKYIQ 342

Query: 361 SIKRLMTFCQRRYPTMPSKAVEFNSL 384
            +KRLMT CQ+ +PT PSK VE+N+L
Sbjct: 361 GLKRLMTICQKHFPTDPSKCVEYNAL 342

[Close]

BLAST of CmaCh08G003450 vs. ExPASy Swiss-Prot
Match: Q5RE03 (Pre-mRNA-splicing factor 18 OS=Pongo abelii OX=9601 GN=PRPF18 PE=2 SV=1)

HSP 1 Score: 186.0 bits (471), Expect = 8.5e-46
Identity = 135/386 (34.97%), Postives = 203/386 (52.59%), Query Frame = 0


Query: 1   MDLLKQELLKKRQSLA---TATGGKRFFKRSEIEQKQIQQLREKEKRELETKALRQSTSS 60
           MD+LK E+L+KRQ +         K++FKRSE+ +K+ +   E+    ++ K   Q    
Sbjct: 1   MDILKSEILRKRQLVEDRNLLVENKKYFKRSELAKKEEEAYFERCGYRIQPKEEDQK--- 60

Query: 61  AATAEANSTSSKSNSLASASTTSSTTINSAATTSKSLTDEQNIDNLVLPRQEVIRRLRFL 120
                                TSS  +       + L        + L RQEVIRRLR  
Sbjct: 61  -------------------PLTSSNPVLELELAEEKLP-------MTLSRQEVIRRLRER 120

Query: 121 KQPITLFGEDDDTRLDRLKYVLKAGLFEVDSDLTEGQTNDFLRDIAELRKRQKTGILSER 180
            +PI LFGE D     RL+ +       +  ++ +G  ND    + ++ ++    I+   
Sbjct: 121 GEPIRLFGETDYDAFQRLRKI-----EILTPEVNKGLRNDLKAALDKIDQQYLNEIV--- 180

Query: 181 KRQKKEDGAGEEGEGGGADEESADGGSSGVDADKDLKRMKANFDELCQEDKILVFFKRLL 240
                  G  E GE    ++      ++ ++  + L       D+    D I  F K LL
Sbjct: 181 -------GGQEPGEEDTQNDLKVHEENTTIEELEALGESLGKGDDHKDMDIITKFLKFLL 240

Query: 241 NEWNQELDEMPEAEKRTAKGKSMVATFKQCARYLHPLFKFCRKKILPDDIRQALLVVVEC 300
             W +EL+   +  KR+ +GK   AT KQ   YL PLF+  RK+ LP DI++++  +++ 
Sbjct: 241 GVWAKELNAREDYVKRSVQGKLNSATQKQTESYLRPLFRKLRKRNLPADIKESITDIIKF 300

Query: 301 CMERDYLAAMDQYIKMAIGNAPWPIGVTMVGIHERSAREKIYTNSVAHIMNDETTRKYLQ 360
            ++R+Y+ A D Y++MAIGNAPWPIGVTMVGIH R+ REKI++  VAH++NDET RKY+Q
Sbjct: 301 MLQREYVKANDAYLQMAIGNAPWPIGVTMVGIHARTGREKIFSKHVAHVLNDETQRKYIQ 342

Query: 361 SIKRLMTFCQRRYPTMPSKAVEFNSL 384
            +KRLMT CQ+ +PT PSK VE+N+L
Sbjct: 361 GLKRLMTICQKHFPTDPSKCVEYNAL 342

[Close]

BLAST of CmaCh08G003450 vs. ExPASy Swiss-Prot
Match: Q2HJ41 (Pre-mRNA-splicing factor 18 OS=Bos taurus OX=9913 GN=PRPF18 PE=2 SV=1)

HSP 1 Score: 184.9 bits (468), Expect = 1.9e-45
Identity = 134/386 (34.72%), Postives = 204/386 (52.85%), Query Frame = 0


Query: 1   MDLLKQELLKKRQSLA---TATGGKRFFKRSEIEQKQIQQLREKEKRELETKALRQSTSS 60
           MD+LK E+L+KRQ +         K++FKRSE+ +K+ +   E+   +++ K   Q    
Sbjct: 1   MDVLKSEILRKRQLVEDRNLLVENKKYFKRSELAKKEEEAYFERCGYKIQPKDEDQK--- 60

Query: 61  AATAEANSTSSKSNSLASASTTSSTTINSAATTSKSLTDEQNIDNLVLPRQEVIRRLRFL 120
                                TSS  +       + L        + L RQEVIRRLR  
Sbjct: 61  -------------------PLTSSNPVLELELAEEKLP-------MTLSRQEVIRRLRER 120

Query: 121 KQPITLFGEDDDTRLDRLKYVLKAGLFEVDSDLTEGQTNDFLRDIAELRKRQKTGILSER 180
            +PI LFGE D     RL+ +       +  ++ +G  ND    + ++ ++    ++   
Sbjct: 121 GEPIRLFGETDYDAFQRLRKI-----EILTPEVNKGLRNDLKAALDKIDQQYLNELV--- 180

Query: 181 KRQKKEDGAGEEGEGGGADEESADGGSSGVDADKDLKRMKANFDELCQEDKILVFFKRLL 240
                  G  E GE    ++      ++ ++  + L       D+    D I  F K LL
Sbjct: 181 -------GGQEPGEEDTQNDLKVHEENTTIEELEALGESLGKGDDHKDMDIITKFLKFLL 240

Query: 241 NEWNQELDEMPEAEKRTAKGKSMVATFKQCARYLHPLFKFCRKKILPDDIRQALLVVVEC 300
             W +EL+   +  KR+ +GK   AT KQ   YL PLF+  RK+ LP DI++++  +++ 
Sbjct: 241 GVWAKELNAREDYVKRSVQGKLNSATQKQTESYLRPLFRKLRKRNLPADIKESITDIIKF 300

Query: 301 CMERDYLAAMDQYIKMAIGNAPWPIGVTMVGIHERSAREKIYTNSVAHIMNDETTRKYLQ 360
            ++R+Y+ A D Y++MAIGNAPWPIGVTMVGIH R+ REKI++  VAH++NDET RKY+Q
Sbjct: 301 MLQREYVKANDAYLQMAIGNAPWPIGVTMVGIHARTGREKIFSKHVAHVLNDETQRKYIQ 342

Query: 361 SIKRLMTFCQRRYPTMPSKAVEFNSL 384
            +KRLMT CQ+ +PT PSK VE+N+L
Sbjct: 361 GLKRLMTICQKHFPTDPSKCVEYNAL 342

[Close]

BLAST of CmaCh08G003450 vs. ExPASy Swiss-Prot
Match: Q9JKB8 (Pre-mRNA-splicing factor 18 OS=Rattus norvegicus OX=10116 GN=Prpf18 PE=2 SV=1)

HSP 1 Score: 184.9 bits (468), Expect = 1.9e-45
Identity = 134/386 (34.72%), Postives = 204/386 (52.85%), Query Frame = 0


Query: 1   MDLLKQELLKKRQSLA---TATGGKRFFKRSEIEQKQIQQLREKEKRELETKALRQSTSS 60
           MD+LK E+L+KRQ +         K++FKRSE+ +K+ +   E+   +++ K   Q    
Sbjct: 1   MDILKSEILRKRQLVEDRNLLVENKKYFKRSELAKKEEEAYFERCGYKVQPKEDDQK--- 60

Query: 61  AATAEANSTSSKSNSLASASTTSSTTINSAATTSKSLTDEQNIDNLVLPRQEVIRRLRFL 120
                                TSS  +       + L        + L RQEVIRRLR  
Sbjct: 61  -------------------PLTSSNPVLELELAEEKLP-------MTLSRQEVIRRLRER 120

Query: 121 KQPITLFGEDDDTRLDRLKYVLKAGLFEVDSDLTEGQTNDFLRDIAELRKRQKTGILSER 180
            +P+ LFGE D     RL+ +       +  ++ +G  ND    + ++ ++    I+   
Sbjct: 121 GEPMRLFGETDYDAFQRLRKI-----EILTPEVNKGLRNDLKAALDKIDQQYLNEIV--- 180

Query: 181 KRQKKEDGAGEEGEGGGADEESADGGSSGVDADKDLKRMKANFDELCQEDKILVFFKRLL 240
                  G  E GE    ++      ++ ++  + L       D+    D I  F K LL
Sbjct: 181 -------GGQEPGEEDTQNDLKVHEENTTIEELEALGESLGKGDDHKDMDIITKFLKFLL 240

Query: 241 NEWNQELDEMPEAEKRTAKGKSMVATFKQCARYLHPLFKFCRKKILPDDIRQALLVVVEC 300
             W +EL+   +  KR+ +GK   AT KQ   YL PLF+  RK+ LP DI++++  +++ 
Sbjct: 241 GVWAKELNSREDYVKRSVQGKLNSATQKQTESYLRPLFRKLRKRNLPADIKESITDIIKF 300

Query: 301 CMERDYLAAMDQYIKMAIGNAPWPIGVTMVGIHERSAREKIYTNSVAHIMNDETTRKYLQ 360
            ++R+Y+ A D Y++MAIGNAPWPIGVTMVGIH R+ REKI++  VAH++NDET RKY+Q
Sbjct: 301 MLQREYVKANDAYLQMAIGNAPWPIGVTMVGIHARTGREKIFSKHVAHVLNDETQRKYIQ 342

Query: 361 SIKRLMTFCQRRYPTMPSKAVEFNSL 384
            +KRLMT CQ+ +PT PSK VE+N+L
Sbjct: 361 GLKRLMTICQKHFPTDPSKCVEYNAL 342

[Close]

BLAST of CmaCh08G003450 vs. TAIR 10
Match: AT1G03140.1 (splicing factor Prp18 family protein )

HSP 1 Score: 547.7 bits (1410), Expect = 7.9e-156
Identity = 301/424 (70.99%), Postives = 356/424 (83.96%), Query Frame = 0


Query: 1   MDLLKQELLKKRQSLATATGGKRFFKRSEIEQKQIQQLREKEKRELETKALRQSTSSAAT 60
           MDLL++E+LKKR+SLA  +GGK+FFKRSEIEQK+IQ+LRE+E+RE E KA R++ ++A+ 
Sbjct: 1   MDLLREEILKKRKSLAEESGGKKFFKRSEIEQKKIQKLREEERREHELKAQRRAAAAASG 60

Query: 61  AEANSTSS---KSNSLASASTTSSTTINSAATTSKSLTDEQNIDNLVLPRQEVIRRLRFL 120
            +  S+ S    SN+  SAS+ SS +  +A   SK+LTDE    NL+LPRQEVIRRLRFL
Sbjct: 61  GDGKSSGSAPGSSNAATSASSKSSASDAAAIADSKALTDE----NLILPRQEVIRRLRFL 120

Query: 121 KQPITLFGEDDDTRLDRLKYVLKAGLFEVDSDLTEGQTNDFLRDIAELRKRQKTGILSER 180
           KQP+TLFGEDD +RLDRLKYVLK GLFEVDSD+TEGQTNDFLRDIAEL+KRQK+G++ +R
Sbjct: 121 KQPMTLFGEDDQSRLDRLKYVLKEGLFEVDSDMTEGQTNDFLRDIAELKKRQKSGMMGDR 180

Query: 181 KRQKKEDGAGEEGEGGGADEESADGG-SSGVDADKDLKRMKANFDELCQEDKILVFFKRL 240
           KR+ +++   +EG+ G   E+   GG SS VDADKD+KR+KANF++LC EDKILVF+K+L
Sbjct: 181 KRKSRDERGRDEGDRGETREDELSGGESSDVDADKDMKRLKANFEDLCDEDKILVFYKKL 240

Query: 241 LNEWNQELDEMPEAEKRTAKGKSMVATFKQCARYLHPLFKFCRKKILPDDIRQALLVVVE 300
           L EW QELD M   E+RTAKGK MVATFKQCARYL PLF  CRKK LP DIRQAL+V+V 
Sbjct: 241 LIEWKQELDAMENTERRTAKGKQMVATFKQCARYLVPLFNLCRKKGLPADIRQALMVMVN 300

Query: 301 CCMERDYLAAMDQYIKMAIGNAPWPIGVTMVGIHERSAREKIYTNSVAHIMNDETTRKYL 360
            C++RDYLAAMD YIK+AIGNAPWPIGVTMVGIHERSAREKIYTNSVAHIMNDETTRKYL
Sbjct: 301 HCIKRDYLAAMDHYIKLAIGNAPWPIGVTMVGIHERSAREKIYTNSVAHIMNDETTRKYL 360

Query: 361 QSIKRLMTFCQRRYPTMPSKAVEFNSLANGSDLQSLLAEERVSGGWGKQGSDERLRIMHS 420
           QS+KRLMTFCQRRYPTMPSKAVEFNSLANGSDLQSLLAEER  GG  +Q S+ERLR+M S
Sbjct: 361 QSVKRLMTFCQRRYPTMPSKAVEFNSLANGSDLQSLLAEERFFGGNREQVSEERLRLMPS 420

[Close]

BLAST of CmaCh08G003450 vs. TAIR 10
Match: AT1G54590.1 (INVOLVED IN: RNA splicing; LOCATED IN: spliceosomal complex; CONTAINS InterPro DOMAIN/s: Prp18 (InterPro:IPR004098); BEST Arabidopsis thaliana protein match is: splicing factor Prp18 family protein (TAIR:AT1G03140.1); Has 388 Blast hits to 388 proteins in 196 species: Archae - 0; Bacteria - 0; Metazoa - 136; Fungi - 132; Plants - 48; Viruses - 0; Other Eukaryotes - 72 (source: NCBI BLink). )

HSP 1 Score: 329.7 bits (844), Expect = 3.4e-90
Identity = 183/290 (63.10%), Postives = 223/290 (76.90%), Query Frame = 0


Query: 131 RLDRLKYVLKAGLF-EVDSDLTEGQTNDFLRDIAELRKRQK-TGILSERKRQKKEDGAGE 190
           RLDRLKYVLK GLF EVD+D+T+G+TNDFLRDI EL+KR++ +G++++RKR+   D    
Sbjct: 86  RLDRLKYVLKEGLFEEVDNDMTQGETNDFLRDITELKKRERSSGLMNDRKRKTSND---- 145

Query: 191 EGEGGGADEESADGGSSGVDADKDLKRM-KANFDELCQEDKILVFFKRLLNEWNQELDEM 250
             E  GA++E             DLK + +ANF++LC EDKILVF K+LL EW QEL+ M
Sbjct: 146 -DELIGAEKE-------------DLKLLEEANFEDLCDEDKILVFCKKLLLEWKQELEAM 205

Query: 251 PEAEKRTAKGKSMVATFKQCARYLHPLFKFCRKKILPDDIRQALLVVVECCMERDYLAAM 310
              E+RTA GK M+ATF QCARYL PLF  CR K LP DIRQ L+V+V C ++RDYL A 
Sbjct: 206 ENTERRTAIGKQMLATFNQCARYLTPLFHLCRNKCLPADIRQGLMVMVNCWIKRDYLDAT 265

Query: 311 DQYIKMAIGNAPWPIGVTMVGIHERSAREKIYT-NSVAHIMNDETTRKYLQSIKRLMTFC 370
            Q+IK+AIGNAPWPIGVTMVGIHERSAREKI T +SVAHIMN+ETTRKYLQS+KRLMTFC
Sbjct: 266 AQFIKLAIGNAPWPIGVTMVGIHERSAREKISTSSSVAHIMNNETTRKYLQSVKRLMTFC 325

Query: 371 QRRYPTMPSKAVEFNSLANGSDLQSLLAEERVSGGWGKQGSDERLRIMHS 417
           QRRY  +PSK++EFNSLANGS+L SLLAEER      ++ S+ERL +M S
Sbjct: 326 QRRYSALPSKSIEFNSLANGSNLHSLLAEERFFAADRERVSEERLWLMPS 357

The following BLAST results are available for this feature:

BLAST of CmaCh08G003450 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q99633	6.5e-46	34.97	Pre-mRNA-splicing factor 18 OS=Homo sapiens OX=9606 GN=PRPF18 PE=1 SV=1	[more]
Q8BM39	6.5e-46	34.97	Pre-mRNA-splicing factor 18 OS=Mus musculus OX=10090 GN=Prpf18 PE=1 SV=1	[more]
Q5RE03	8.5e-46	34.97	Pre-mRNA-splicing factor 18 OS=Pongo abelii OX=9601 GN=PRPF18 PE=2 SV=1	[more]
Q2HJ41	1.9e-45	34.72	Pre-mRNA-splicing factor 18 OS=Bos taurus OX=9913 GN=PRPF18 PE=2 SV=1	[more]
Q9JKB8	1.9e-45	34.72	Pre-mRNA-splicing factor 18 OS=Rattus norvegicus OX=10116 GN=Prpf18 PE=2 SV=1	[more]

BLAST of CmaCh08G003450 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G03140.1	7.9e-156	70.99	splicing factor Prp18 family protein	[more]
AT1G54590.1	3.4e-90	63.10	INVOLVED IN: RNA splicing; LOCATED IN: spliceosomal complex; CONTAINS InterPro D...	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR014906	Pre-mRNA processing factor 4 (PRP4)-like	SMART	SM00500	pr04_2	coord: 105..156 e-value: 9.6E-22 score: 88.2
IPR014906	Pre-mRNA processing factor 4 (PRP4)-like	PFAM	PF08799	PRP4	coord: 110..137 e-value: 3.1E-11 score: 42.6
None	No IPR available	GENE3D	1.20.940.10	Functional domain of the splicing factor Prp18	coord: 203..374 e-value: 4.1E-59 score: 201.3
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 174..189
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 36..50
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 400..420
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 51..86
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 36..86
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 174..208
None	No IPR available	PANTHER	PTHR13007:SF22	BNAA09G50970D PROTEIN	coord: 1..415
None	No IPR available	SUPERFAMILY	47938	Functional domain of the splicing factor Prp18	coord: 206..372
IPR004098	Prp18	PFAM	PF02840	Prp18	coord: 231..372 e-value: 2.8E-62 score: 209.0
IPR036285	PRP4-like superfamily	GENE3D	4.10.280.110		coord: 76..153 e-value: 4.7E-16 score: 60.0
IPR036285	PRP4-like superfamily	SUPERFAMILY	158230	PRP4-like	coord: 98..140
IPR039979	Pre-mRNA-splicing factor 18	PANTHER	PTHR13007	PRE-MRNA SPLICING FACTOR-RELATED	coord: 1..415

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmaCh08G003450.1	CmaCh08G003450.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0000350	generation of catalytic spliceosome for second transesterification step
biological_process	GO:0008380	RNA splicing
cellular_component	GO:0071021	U2-type post-spliceosomal complex
cellular_component	GO:0046540	U4/U6 x U5 tri-snRNP complex
cellular_component	GO:0005682	U5 snRNP
cellular_component	GO:0005681	spliceosomal complex