CmaCh08G003240 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh08G003240
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
DescriptionFe2OG dioxygenase domain-containing protein
LocationCma_Chr08: 1858248 .. 1860275 (+)
RNA-Seq ExpressionCmaCh08G003240
SyntenyCmaCh08G003240
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
GATTTTCGACAGCTCAGTTCTGGTTTAAACGGTCATTCATTCATATTGTTCTTCCCAACGGGACGAAACCCTAGATTCTGGATTGAGAAGTGCAAGAACAGAGAAAAATGGAGGAAAATCCCAATAAACTCGAAATTTATGAGCTCAGGTATTCGGAACTCTTCCTCTTGTCTACACATTGCCACTCTTCATCTTCATTACAAGATAACGAACGGATTGAATCGATAACCAAATCGATTTTTGAAGCCCTAGGGCCCAGTGGACCTGGCCTTCTCGCAATCACCGGCGTCCCCAACTCTTCTGTTCTTCGTCGAACACTACTGCCTCTCGCTCGCAAGCTCGCATTGCTCAATCCTGATGATCGGAAACGGATTCTTAAGGTTTTACTTCTTCATTTTTCCTCATTCCTCTGCTTTCTCTTGCTCGCTTCCTTTTTTGTAAGCATTTAGTGAATGTTTACGGAAAATGATGAGGAATCTGATGTTGACGGCGCTCTTTTTTTCCCTAAAATCTCGTCATTATCACTTTGTTGAAATCATGTTGAACATAACCGAAATGGGAAGTGGAATAATAGCGGATCATTGGGAAATGTCTCGAGATAATGTCATAGTTGTATTGATTTAAACCTTCTTCATACGTCATGACTATTGGAGATTAAATTTTGTTGAATTCTAGTTGATCTTTTGACATTTCTTTGTTTGATGTTTAACTTAAACGATTTAGCGAATCTGAGTTTCGGGGTACTTTTGGTTGGTTGGTTAGGTTTCTCAAAATTAAAATTCTCTGAAGCTTGTGATCAAATCGTTGAAGAGTCATTTTTTTGTTTGGCTCTCGTGAGATCTTAGCTAAATTCTTGTTCGCTACGAACATTTTACCATAACATCCTTGGAAGGAATCAAATTCATATTCAAATGAGACAATCTTCCTAATACCTCCATTTCTTTTCATTCAACTTCTCAGGATCATTACTTAGGGAGTGATGTTCCCTTGAGGAATCCAGAAAGAAGTGTTTCGTCTTTTGCAATGCAACTCAAATTTACGGAGAGCAAAGAATTCATGCAGAATAGTCAAAGCCAAAGAGGGGACAAACAGTCACCTGGTTCAGAAATTGATCACTTTTGTGATTCTATTGAGAAGGAAGTTCAAGACAATGAATTTAAACATCTTGGTGATTCATTTAAAGAGCTTGGAAGTTGCATGCTGGAACTGGGGCTTCGCATTGCACGGATATGTGATGCGCAAATCGGAGGCCAAGAATTAGAACAGAGCTTGTTAGAGTCATGCACTGCAAAAGGCCGTCTCATACACTACCATTCAGCTCTGGATGCCCAGCTTTTAAGAAAATCAACAAACTTCAAAGGAACTGCAAGAAACCAAGCTAATTCTAGAAGAAATAAAGAACAAAGCATACACAGTAGGCAAGAGCCATCAGACAGTAATGGACTATGTCAATCAAGTACAAATCTATGGCAGCAATGGCATTATGACTATGGTATTTTCACAGTCCTAACAACTCCCATGTTTCTTTCACCATCAAATACCCACGGCAACGAAGCACAAGATCTTTGTTGCTATAGAGAGTGTTGTTCCCCCAGTGGACACTTGTATTTGCAGATTTTTGATCCTTGTAAGAACGACATTTTCATGGTTAATGCCCCACCAGAAAGCTTTATCATCCAGGTGGGCGAATCGGCTGATATTATATCACGAGGGAAGCTTCGCTCCACTCTTCACTCTGTGTGCAGACCTTCCAAGCACGAGGATTTGTGCAGAGAAATGTTTGTTGTATTCTTGCAGCCAGCTTGGAACAAAACGTTTTCAATGTCTGATTATTCCATTGAAAGCTCAACTTTGTCCGACGACAAAAGAGATCTTGTTGAACAGGAGGCAACGGTAATAACTCGAGAAATCCAGAAGATAGTTCCACCATTAGCATCAAGATTGAAGGAAGGGATGACATTTGCAGAGTTCTCACGTGAAACCACCAAGCAATATTACGGGGGAAATGGTTTGCAATCCAACAGATGA

mRNA sequence

GATTTTCGACAGCTCAGTTCTGGTTTAAACGGTCATTCATTCATATTGTTCTTCCCAACGGGACGAAACCCTAGATTCTGGATTGAGAAGTGCAAGAACAGAGAAAAATGGAGGAAAATCCCAATAAACTCGAAATTTATGAGCTCAGGTATTCGGAACTCTTCCTCTTGTCTACACATTGCCACTCTTCATCTTCATTACAAGATAACGAACGGATTGAATCGATAACCAAATCGATTTTTGAAGCCCTAGGGCCCAGTGGACCTGGCCTTCTCGCAATCACCGGCGTCCCCAACTCTTCTGTTCTTCGTCGAACACTACTGCCTCTCGCTCGCAAGCTCGCATTGCTCAATCCTGATGATCGGAAACGGATTCTTAAGGATCATTACTTAGGGAGTGATGTTCCCTTGAGGAATCCAGAAAGAAGTGTTTCGTCTTTTGCAATGCAACTCAAATTTACGGAGAGCAAAGAATTCATGCAGAATAGTCAAAGCCAAAGAGGGGACAAACAGTCACCTGGTTCAGAAATTGATCACTTTTGTGATTCTATTGAGAAGGAAGTTCAAGACAATGAATTTAAACATCTTGGTGATTCATTTAAAGAGCTTGGAAGTTGCATGCTGGAACTGGGGCTTCGCATTGCACGGATATGTGATGCGCAAATCGGAGGCCAAGAATTAGAACAGAGCTTGTTAGAGTCATGCACTGCAAAAGGCCGTCTCATACACTACCATTCAGCTCTGGATGCCCAGCTTTTAAGAAAATCAACAAACTTCAAAGGAACTGCAAGAAACCAAGCTAATTCTAGAAGAAATAAAGAACAAAGCATACACAGTAGGCAAGAGCCATCAGACAGTAATGGACTATGTCAATCAAGTACAAATCTATGGCAGCAATGGCATTATGACTATGGTATTTTCACAGTCCTAACAACTCCCATGTTTCTTTCACCATCAAATACCCACGGCAACGAAGCACAAGATCTTTGTTGCTATAGAGAGTGTTGTTCCCCCAGTGGACACTTGTATTTGCAGATTTTTGATCCTTGTAAGAACGACATTTTCATGGTTAATGCCCCACCAGAAAGCTTTATCATCCAGGTGGGCGAATCGGCTGATATTATATCACGAGGGAAGCTTCGCTCCACTCTTCACTCTGTGTGCAGACCTTCCAAGCACGAGGATTTGTGCAGAGAAATGTTTGTTGTATTCTTGCAGCCAGCTTGGAACAAAACGTTTTCAATGTCTGATTATTCCATTGAAAGCTCAACTTTGTCCGACGACAAAAGAGATCTTGTTGAACAGGAGGCAACGGTAATAACTCGAGAAATCCAGAAGATAGTTCCACCATTAGCATCAAGATTGAAGGAAGGGATGACATTTGCAGAGTTCTCACGTGAAACCACCAAGCAATATTACGGGGGAAATGGTTTGCAATCCAACAGATGA

Coding sequence (CDS)

ATGGAGGAAAATCCCAATAAACTCGAAATTTATGAGCTCAGGTATTCGGAACTCTTCCTCTTGTCTACACATTGCCACTCTTCATCTTCATTACAAGATAACGAACGGATTGAATCGATAACCAAATCGATTTTTGAAGCCCTAGGGCCCAGTGGACCTGGCCTTCTCGCAATCACCGGCGTCCCCAACTCTTCTGTTCTTCGTCGAACACTACTGCCTCTCGCTCGCAAGCTCGCATTGCTCAATCCTGATGATCGGAAACGGATTCTTAAGGATCATTACTTAGGGAGTGATGTTCCCTTGAGGAATCCAGAAAGAAGTGTTTCGTCTTTTGCAATGCAACTCAAATTTACGGAGAGCAAAGAATTCATGCAGAATAGTCAAAGCCAAAGAGGGGACAAACAGTCACCTGGTTCAGAAATTGATCACTTTTGTGATTCTATTGAGAAGGAAGTTCAAGACAATGAATTTAAACATCTTGGTGATTCATTTAAAGAGCTTGGAAGTTGCATGCTGGAACTGGGGCTTCGCATTGCACGGATATGTGATGCGCAAATCGGAGGCCAAGAATTAGAACAGAGCTTGTTAGAGTCATGCACTGCAAAAGGCCGTCTCATACACTACCATTCAGCTCTGGATGCCCAGCTTTTAAGAAAATCAACAAACTTCAAAGGAACTGCAAGAAACCAAGCTAATTCTAGAAGAAATAAAGAACAAAGCATACACAGTAGGCAAGAGCCATCAGACAGTAATGGACTATGTCAATCAAGTACAAATCTATGGCAGCAATGGCATTATGACTATGGTATTTTCACAGTCCTAACAACTCCCATGTTTCTTTCACCATCAAATACCCACGGCAACGAAGCACAAGATCTTTGTTGCTATAGAGAGTGTTGTTCCCCCAGTGGACACTTGTATTTGCAGATTTTTGATCCTTGTAAGAACGACATTTTCATGGTTAATGCCCCACCAGAAAGCTTTATCATCCAGGTGGGCGAATCGGCTGATATTATATCACGAGGGAAGCTTCGCTCCACTCTTCACTCTGTGTGCAGACCTTCCAAGCACGAGGATTTGTGCAGAGAAATGTTTGTTGTATTCTTGCAGCCAGCTTGGAACAAAACGTTTTCAATGTCTGATTATTCCATTGAAAGCTCAACTTTGTCCGACGACAAAAGAGATCTTGTTGAACAGGAGGCAACGGTAATAACTCGAGAAATCCAGAAGATAGTTCCACCATTAGCATCAAGATTGAAGGAAGGGATGACATTTGCAGAGTTCTCACGTGAAACCACCAAGCAATATTACGGGGGAAATGGTTTGCAATCCAACAGATGA

Protein sequence

MEENPNKLEIYELRYSELFLLSTHCHSSSSLQDNERIESITKSIFEALGPSGPGLLAITGVPNSSVLRRTLLPLARKLALLNPDDRKRILKDHYLGSDVPLRNPERSVSSFAMQLKFTESKEFMQNSQSQRGDKQSPGSEIDHFCDSIEKEVQDNEFKHLGDSFKELGSCMLELGLRIARICDAQIGGQELEQSLLESCTAKGRLIHYHSALDAQLLRKSTNFKGTARNQANSRRNKEQSIHSRQEPSDSNGLCQSSTNLWQQWHYDYGIFTVLTTPMFLSPSNTHGNEAQDLCCYRECCSPSGHLYLQIFDPCKNDIFMVNAPPESFIIQVGESADIISRGKLRSTLHSVCRPSKHEDLCREMFVVFLQPAWNKTFSMSDYSIESSTLSDDKRDLVEQEATVITREIQKIVPPLASRLKEGMTFAEFSRETTKQYYGGNGLQSNR
Homology
BLAST of CmaCh08G003240 vs. TAIR 10
Match: AT3G63290.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 414.1 bits (1063), Expect = 1.4e-115
Identity = 230/439 (52.39%), Postives = 293/439 (66.74%), Query Frame = 0

Query: 8   LEIYELRYSELFLLSTHCHSSSSLQDNERIESITKSIFEALGPSGPGLLAITGVPNSSVL 67
           LE Y+L +S+L L S             R + I+K++ +ALGP+GPGLL ITGV  S+ L
Sbjct: 7   LEPYQLSFSDLLLSS-----------RNRSQWISKNVMDALGPTGPGLLCITGVLGSAFL 66

Query: 68  RRTLLPLARKLALLNPDDRKRILKDHYLGSDVPLRNPERSVSSFAMQLKFTESKEFMQNS 127
           RR LLP+ARKLALL+PD RK IL +H+LGSDVPL+NPER VSSFAMQL +  +      S
Sbjct: 67  RRKLLPMARKLALLDPDKRKLILMEHHLGSDVPLKNPERDVSSFAMQLNYERT---TYKS 126

Query: 128 QSQRGDKQSPGSEIDHFCDSIEKEVQDNEFKHLGDSFKELGSCMLELGLRIARICDAQIG 187
              +      GS++D       +E  D+ F +LG +FKELG CM ELGL IAR+CD +IG
Sbjct: 127 SLGKLWFDEAGSKLD------LQEDDDDAFTNLGGAFKELGFCMRELGLSIARLCDREIG 186

Query: 188 GQELEQSLLESCTAKGRLIHYHSALDAQLLRKSTNFKGTARNQANSRRNKEQSIHSRQEP 247
           G  LE+SLL+SCTAKGRLIHYHSA D   LR+S   +  + N+ +S+R  + +       
Sbjct: 187 GGLLEESLLDSCTAKGRLIHYHSAADKYALRESQR-RNQSGNRVSSKRRVQNAAEQELNR 246

Query: 248 SDSNGLCQSSTNLWQQWHYDYGIFTVLTTPMFLSPSNTHGNEAQDLCCYRECCSPSGHLY 307
            +  GL  S  NLWQQWHYDYGIFTVLT PMFLSP +           Y+E    S H Y
Sbjct: 247 RNGAGLSGSHFNLWQQWHYDYGIFTVLTDPMFLSPYS-----------YQEFSLMSSHSY 306

Query: 308 LQIFDPCKNDIFMVNAPPESFIIQVGESADIISRGKLRSTLHSVCRPSKHEDLCREMFVV 367
           LQI+ P KN  +MV  P +SF++Q+GESADI+S+GKLRSTLH VC+P K + + RE FVV
Sbjct: 307 LQIYHPSKNKFYMVKTPQDSFLVQIGESADILSKGKLRSTLHCVCKPEKLDHVSRETFVV 366

Query: 368 FLQPAWNKTFSMSDYSIESSTLSDDKRDLVEQEATVITREIQKIVPPLASRLKEGMTFAE 427
           FL P W++TFS+S+Y++E           +  +  V   ++Q IVPPL+SRL++GMTFAE
Sbjct: 367 FLHPKWSQTFSVSEYTMEH----------LRSDEVVPRPDLQNIVPPLSSRLRDGMTFAE 403

Query: 428 FSRETTKQYYGGNGLQSNR 447
           FSRETTKQYYGGNGLQSNR
Sbjct: 427 FSRETTKQYYGGNGLQSNR 403

BLAST of CmaCh08G003240 vs. TAIR 10
Match: AT3G63290.2 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 313.2 bits (801), Expect = 3.5e-85
Identity = 162/297 (54.55%), Postives = 205/297 (69.02%), Query Frame = 0

Query: 150 KEVQDNEFKHLGDSFKELGSCMLELGLRIARICDAQIGGQELEQSLLESCTAKGRLIHYH 209
           +E  D+ F +LG +FKELG CM ELGL IAR+CD +IGG  LE+SLL+SCTAKGRLIHYH
Sbjct: 29  QEDDDDAFTNLGGAFKELGFCMRELGLSIARLCDREIGGGLLEESLLDSCTAKGRLIHYH 88

Query: 210 SALDAQLLRKSTNFKGTARNQANSRRNKEQSIHSRQEPSDSNGLCQSSTNLWQQWHYDYG 269
           SA D   LR+S   +  + N+ +S+R  + +        +  GL  S  NLWQQWHYDYG
Sbjct: 89  SAADKYALRESQR-RNQSGNRVSSKRRVQNAAEQELNRRNGAGLSGSHFNLWQQWHYDYG 148

Query: 270 IFTVLTTPMFLSPSNTHGNEAQDLCCYRECCSPSGHLYLQIFDPCKNDIFMVNAPPESFI 329
           IFTVLT PMFLSP +           Y+E    S H YLQI+ P KN  +MV  P +SF+
Sbjct: 149 IFTVLTDPMFLSPYS-----------YQEFSLMSSHSYLQIYHPSKNKFYMVKTPQDSFL 208

Query: 330 IQVGESADIISRGKLRSTLHSVCRPSKHEDLCREMFVVFLQPAWNKTFSMSDYSIESSTL 389
           +Q+GESADI+S+GKLRSTLH VC+P K + + RE FVVFL P W++TFS+S+Y++E    
Sbjct: 209 VQIGESADILSKGKLRSTLHCVCKPEKLDHVSRETFVVFLHPKWSQTFSVSEYTMEH--- 268

Query: 390 SDDKRDLVEQEATVITREIQKIVPPLASRLKEGMTFAEFSRETTKQYYGGNGLQSNR 447
                  +  +  V   ++Q IVPPL+SRL++GMTFAEFSRETTKQYYGGNGLQSNR
Sbjct: 269 -------LRSDEVVPRPDLQNIVPPLSSRLRDGMTFAEFSRETTKQYYGGNGLQSNR 303

BLAST of CmaCh08G003240 vs. TAIR 10
Match: AT4G13400.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 90.1 bits (222), Expect = 4.8e-18
Identity = 105/383 (27.42%), Postives = 152/383 (39.69%), Query Frame = 0

Query: 21  LSTHCHSSSSLQDNERIESITKSIFEALGPSGPGLLAITGVPNSSVLRRTLLPLARKLAL 80
           +ST   S S L+++     ++  I E  GP+G G+L++  VP  S LR+ LL LA +LA 
Sbjct: 12  VSTVTISYSELKESN--IDLSARIEEGFGPNGLGILSVKDVPGYSALRQNLLQLAPRLAG 71

Query: 81  LNPDDRKRILKDHYLGSDVPLRNPERSVSSFAMQLKFTESKEFMQNSQSQRGDKQSPGSE 140
           L P++ KR L+D +   +    + +  + S   +L   +   +    Q            
Sbjct: 72  L-PEEVKRELEDPHSRYNFGWSHGKEKLES--GKLDMLKGSYYANPLQDVPTSNSYEIQR 131

Query: 141 IDHFCDSIEKEVQDNEFKHLGDSFKELGSCMLELGLRIARICDAQIG-------GQELEQ 200
              +C S       N    L  +FK LG  M E+GL +A  CD  +         Q LE+
Sbjct: 132 YPSYCGS--NIWPRNSLPELEGAFKALGKLMFEVGLMVAYHCDQYVSKGIKQHEKQNLEK 191

Query: 201 SLLESCTAKGRLIHYHSALDAQLLRKSTNFKGTARNQANSRRNKEQSIHSRQEPSDSNGL 260
            LL S   KGRL++Y  A                         +E S H           
Sbjct: 192 ILLGSRCHKGRLLYYFPA-------------------------QESSTHD---------- 251

Query: 261 CQSSTNLWQQWHYDYGIFTVLTTPMFLSPSNTHGNEAQDLCCYRECCSPSGHLYLQIFDP 320
              S + W  WH D+G  T LT  +F   S               C  P+  LY+Q    
Sbjct: 252 -NDSISSWCGWHTDHGSLTGLTRAIFSRDS-----------VEVPCPDPASGLYIQ---T 311

Query: 321 CKNDIFMVNAPPESFIIQVGESADIISRGKLRSTLHSVCRPSKHE--DLCREMFVVFLQP 380
               I  V    +    Q+GE+  I+S G L +T H V  P   E   L R  F +F+QP
Sbjct: 312 RSGQIVKVVYGEDEIAYQIGETTSILSSGYLCATPHCVRAPQGEEARGLERSTFALFMQP 337

Query: 381 AWNK--TFSMSDYSIESSTLSDD 393
            W++  TF       E   LSD+
Sbjct: 372 DWDQKLTFPKEVTIHEELCLSDE 337

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
AT3G63290.11.4e-11552.392-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT3G63290.23.5e-8554.552-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT4G13400.14.8e-1827.422-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR027443Isopenicillin N synthase-like superfamilyGENE3D2.60.120.330coord: 25..403
e-value: 4.8E-26
score: 93.9
IPR044861Isopenicillin N synthase-like, Fe(2+) 2OG dioxygenase domainPFAMPF031712OG-FeII_Oxycoord: 309..359
e-value: 2.5E-4
score: 21.5
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 225..253
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 122..141
NoneNo IPR availablePANTHERPTHR10209:SF5532-OXOGLUTARATE (2OG) AND FE(II)-DEPENDENT OXYGENASE SUPERFAMILY PROTEINcoord: 8..423
NoneNo IPR availablePANTHERPTHR10209OXIDOREDUCTASE, 2OG-FE II OXYGENASE FAMILY PROTEINcoord: 8..423
NoneNo IPR availableSUPERFAMILY51197Clavaminate synthase-likecoord: 35..440

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh08G003240.1CmaCh08G003240.1mRNA