CmaCh08G002010 (gene) Cucurbita maxima (Rimu) v1.1

BLAST of CmaCh08G002010 vs. ExPASy Swiss-Prot
Match: Q9LX30 (eIF-2-alpha kinase GCN2 OS=Arabidopsis thaliana OX=3702 GN=GCN2 PE=2 SV=2)

HSP 1 Score: 1506.9 bits (3900), Expect = 0.0e+00
Identity = 781/1238 (63.09%), Postives = 942/1238 (76.09%), Query Frame = 0

Query: 114  SSKKKRRGGGGGGKRSKGRTPQKDH-SFSGEEGELISEELTALCGIFQEDCKVVSG--PS 173
            SS KK++  GG G+R +     KDH S + E+ EL+SEE+TAL  IFQEDCKVVS     
Sbjct: 4    SSSKKKKKRGGSGRRGQ----LKDHGSNADEDNELLSEEITALSAIFQEDCKVVSDSRSP 63

Query: 174  PQVTIKLRPYSNDMGFEDLDVSAFLSVKYLPGYPYKCPKLLITPEKGLAKGDTEKLLSLL 233
            PQ+ IKLRPYS DMG+ED D+SA L V+ LPGYPYKCPKL ITPE+GL   D EKLLSLL
Sbjct: 64   PQIAIKLRPYSKDMGYEDTDISAMLIVRCLPGYPYKCPKLQITPEQGLTTADAEKLLSLL 123

Query: 234  HEQASYNARDGRIMIFNLAEAAQEFLSEIVTIGQSNESVGCSNTDNNSQLLPDKVTSN-- 293
             +QA+ NAR+GR+MIFNL EAAQEFLSEI+      ESV C     ++Q +   + SN  
Sbjct: 124  EDQANSNAREGRVMIFNLVEAAQEFLSEIIPESHDEESVPCLTAHRSTQFIEQPMLSNIA 183

Query: 294  ---EKGPYVHGYIDLFSGSGELWSWSFDMDEK----PYSQAQPLADSLKLGTVQEKSLDK 353
                 GP+V+G+IDLFSG  +  +WS   DE        Q+ PL  S  L    +K+L +
Sbjct: 184  KSCSGGPFVYGFIDLFSGLEDARNWSLTPDENRGIVSSVQSHPLDTSRILHQKPDKNLKR 243

Query: 354  VQNLLTKQNSKRGELLSPSFNLGTLEEESEDESHSITSSHSRRSLIVQKAGDVTEDEKQD 413
             ++   ++ +    L +P   L T++EE+ D++ SI+S  S +S    ++G + ++EK++
Sbjct: 244  FEDHAKEEVA----LPAPIAKLNTVQEENVDDT-SISSFDSSKSTDDVESG-LFQNEKKE 303

Query: 414  SIIVDHAAEDDDHGSESEP-SEWSFASMSKEQESQTTDRDIMMVHLLHLACAPKGPLADA 473
            S + D  AEDD   SESE    WS  S++++Q  Q + +D++MVHLL +AC  +GPLADA
Sbjct: 304  SNLQDDTAEDDSTNSESESLGSWSSDSLAQDQVPQISKKDLLMVHLLRVACTSRGPLADA 363

Query: 474  LPKLASELYNSGILSKVALDLASKPSSTFHKKFESAFQEEMNATSFSEFWN-PSDFGGPT 533
            LP++  EL+  GILS+  LDLASK S  F++ FE AF + M +TS  +FW  PSD   P 
Sbjct: 364  LPQITDELHELGILSEEVLDLASKSSPDFNRTFEHAFNQNMASTSVPQFWEPPSDSCEPN 423

Query: 534  SSQLSSRYLNDFEELKPLGHGGFGHVVLCKNKLDGRQYAVKKIRLKDKILPVDDRILREV 593
            +S  SSRYLNDFEELKPLG GGFGHVVLCKNKLDGRQYAVKKIRLKDK +PV+ RI+REV
Sbjct: 424  ASLPSSRYLNDFEELKPLGQGGFGHVVLCKNKLDGRQYAVKKIRLKDKEIPVNSRIVREV 483

Query: 594  STLSRLQHQHVVRYYQAWSEAGVSDSYGEAAWGSTTPLSSTFSYKGASATDALDHENKLE 653
            +TLSRLQHQHVVRYYQAW E GV D +  A WGS T  SS FSY GA +T+  + +N LE
Sbjct: 484  ATLSRLQHQHVVRYYQAWFETGVVDPFAGANWGSKTAGSSMFSYSGAVSTEIPEQDNNLE 543

Query: 654  STYLYIQMEYCPRTLRQDFESYTHFDKELAWHLFHQIVEGLAHIHGQGIIHRDLTPSNIF 713
            STYLYIQMEYCPRTLRQ FESY HFDK+ AWHL  QIVEGLAHIHGQGIIHRD TP+NIF
Sbjct: 544  STYLYIQMEYCPRTLRQVFESYNHFDKDFAWHLIRQIVEGLAHIHGQGIIHRDFTPNNIF 603

Query: 714  FDARNDIKIGDFGLAKFLKLEQLDQDVGVPLDTTGVSIDGTGQVGTYFYTAPEIEQGWPK 773
            FDARNDIKIGDFGLAKFLKLEQLDQD G   D  G  +D TGQ GTYFYTAPEIEQ WPK
Sbjct: 604  FDARNDIKIGDFGLAKFLKLEQLDQDGGFSTDVAGSGVDSTGQAGTYFYTAPEIEQDWPK 663

Query: 774  IDEKADMYSLGIVFFELWHPFGTAMERHLVLSDLKQKGELPTTWVAEFPEQASLLRRLMS 833
            IDEKADMYSLG+VFFELWHPFGTAMERH++L++LK KGELP  WV EFPEQASLLRRLMS
Sbjct: 664  IDEKADMYSLGVVFFELWHPFGTAMERHVILTNLKLKGELPLKWVNEFPEQASLLRRLMS 723

Query: 834  QSPSERPSALELLQHAFPPRMEYQLLDNILRTMKTSEDSSIYDRVVNAIFSEETLVMKDD 893
             SPS+RPSA ELL+HAFPPRME +LLDNILR M+TSEDSS+YDRVV+ IF EE L MK  
Sbjct: 724  PSPSDRPSATELLKHAFPPRMESELLDNILRIMQTSEDSSVYDRVVSVIFDEEVLEMKSH 783

Query: 894  Q-----LDCGTASIQYTDLGTEVRDHVIDVTRELFRLHCAKHLEISPMHLLDGSAQINRN 953
            Q     L    + IQYT++ TE+RD+V+++T+E+FR HCAKHLE+ PM LL    Q +R 
Sbjct: 784  QSSRSRLCADDSYIQYTEINTELRDYVVEITKEVFRQHCAKHLEVIPMRLLSDCPQFSRK 843

Query: 954  TVKLLSHGGDILELCHELRLPFLNWLVSCQKSSFKRYDIACVYRRAIGHSPPNRYLQGDF 1013
            TVKLL++GGD+LELC+ELRLPF++W+   QKSSFKRY+I+ VYRRAIGHSPPN  LQ DF
Sbjct: 844  TVKLLTNGGDMLELCYELRLPFVHWISVNQKSSFKRYEISHVYRRAIGHSPPNPCLQADF 903

Query: 1014 DIIGGTSALTEAEVIKVTVDVISYFFHSESCDIHLNHGDLLNAIWSWVGVKAEHRHKVAE 1073
            DI+GGT +LTEAEV+KV VD+ ++ FH  SCDIHLNHGDLL+AIWSW G+KAEHR KVAE
Sbjct: 904  DIVGGTLSLTEAEVLKVIVDITTHIFHRGSCDIHLNHGDLLDAIWSWAGIKAEHRRKVAE 963

Query: 1074 LLSMMVSLRPQSSERKSKWVVIRRQLLQELKLAQAVVNRLQTVVFRFCGAVDQAVPRLRG 1133
            LLSMM SLRPQSSERK KWV IRRQLLQELKL +AVVNRLQTV  RFCG  DQA+PRLRG
Sbjct: 964  LLSMMGSLRPQSSERKLKWVFIRRQLLQELKLPEAVVNRLQTVASRFCGDADQALPRLRG 1023

Query: 1134 ALPTDKPMRRALVELLDLFNYLKVWRLEANVYIDALMSPTEDYHRDIFFQVYLTKENHPG 1193
            AL  D+P R+AL EL +L  YL+VWR+E +V+ID LM PTE YHR++FFQV+LTKEN  G
Sbjct: 1024 ALRADRPTRKALDELSNLLTYLRVWRIEEHVHIDVLMPPTESYHRNLFFQVFLTKENSSG 1083

Query: 1194 SHPEGTLVAIGGRYDYLLQQMWSRAYKTTPPGGVGTSIALETMIQQAAVDLKPIRNEGST 1253
            +  +G L+A+GGRYD+L+Q++  R +K   PG VG S+ALET+ Q   +DL+PIRNE ST
Sbjct: 1084 TSNDGVLLAVGGRYDWLVQEVCDREHKMNLPGAVGVSLALETIFQHLPMDLRPIRNEVST 1143

Query: 1254 SFLVCSRGGGGLLLERMELVVELWEEKIKAQLVPTPDPSFTEQYEYANEHDIKCLVIITD 1313
            S LVCSRGGGGLL++RMELV ELWE+ IKA+ VPTPDPS TEQYEYANEH+IKCLVIIT+
Sbjct: 1144 SVLVCSRGGGGLLVQRMELVAELWEKSIKAEFVPTPDPSLTEQYEYANEHEIKCLVIITE 1203

Query: 1314 SGVSNR--GSVRVRHLELKREKEVERTSICFSSLNYLA 1331
            SGV+      V+VRHLELK+EK V R  +    L+ +A
Sbjct: 1204 SGVAQNQIEFVKVRHLELKKEKVVGREELVKFLLDAMA 1231

BLAST of CmaCh08G002010 vs. ExPASy Swiss-Prot
Match: Q9HGN1 (eIF-2-alpha kinase GCN2 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=gcn2 PE=2 SV=2)

HSP 1 Score: 275.4 bits (703), Expect = 3.7e-72
Identity = 280/1015 (27.59%), Postives = 441/1015 (43.45%), Query Frame = 0

Query: 419  EPSEWSFASMSKEQESQTTDRDIMMV----HLLHLACAPKGPLADALPKLASELYNSGIL 478
            E SE SFA+ S++ +       ++ +    H+L+   + K  +   +P L    Y   + 
Sbjct: 430  EVSESSFAAASRKTDIWCFGLLVLQMLCGAHVLNKFSSLKLIMTHVIPLLPGS-YQDLVR 489

Query: 479  SKVALDLASKPS-----STFHKKFESAFQEEMNATSFSEFWNPSDFGGPTSSQLS----- 538
              +  D   +PS     S+   +  +A    +   +FS+   PS +GG     +      
Sbjct: 490  RCLMRDSRKRPSAIDLLSSHVIRLGTAVLPPVEQGTFSKSARPS-YGGQQDGIIDLLYRK 549

Query: 539  --SRYLNDFEELKPLGHGGFGHVVLCKNKLDGRQYAVKKIRL--KDKILPVDDRILREVS 598
              SRY  DFEEL+ LG GGFG VV  KN++DGR YAVKK+ L   DK    + RILREV 
Sbjct: 550  SVSRYETDFEELEFLGRGGFGEVVKVKNRIDGRFYAVKKLVLLSDDK---ENSRILREVM 609

Query: 599  TLSRLQHQHVVRYYQAWSEAGVSDSYGEAAWGSTTPLSS--------------------- 658
            TLSRL H+HVVRYY AW E   +D+  E     +  LS                      
Sbjct: 610  TLSRLHHEHVVRYYTAWVETEANDTVTEIISSDSESLSQSLNMAVDFRQSSSLPADKLSS 669

Query: 659  -----------------------TFSYKGAS--------------ATDALDHENKLESTY 718
                                   +F Y   S              A+     EN L +T 
Sbjct: 670  LDIHFEDDYNSSADEEDPEASDISFQYSNTSDKEGSSDKDSSIEEASSVKTQENGLNAT- 729

Query: 719  LYIQMEYCPRTLRQDFESYTHFDKELAWHLFHQIVEGLAHIHGQGIIHRDLTPSNIFFDA 778
            LYIQMEYC +   QD         +  W LF QI+E LA+IH +G++HRDL P NIF D 
Sbjct: 730  LYIQMEYCEKLSLQDI-IRDKIPVDEMWRLFRQILEALAYIHSRGMMHRDLKPGNIFLDE 789

Query: 779  RNDIKIGDFGLAKFLKLEQLDQDVGVPLDTTGVSIDGTGQVGTYFYTAPEI--EQGWPKI 838
              ++K+GDFGLA   +  Q + D     +      D T  VGT  Y APE+   +   + 
Sbjct: 790  NRNVKLGDFGLATENENYQDNNDKW--KNRQSADEDLTTGVGTALYVAPELLSRRNGVRY 849

Query: 839  DEKADMYSLGIVFFELWHPFGTAMERHLVLSDLKQKG-ELPTTW-VAEFPEQASLLRRLM 898
            D K DMYSLGI+ FE+   F T+MER  ++  ++      P+T+  +    +  ++  L+
Sbjct: 850  DAKVDMYSLGIILFEMCMTFSTSMERIRIIDTIRSPSISFPSTFPFSRASHEFKVIHCLL 909

Query: 899  SQSPSERPSALELLQ-HAFPPRMEYQLLDNILRTMKTSEDSSIYDRVVNAIFSEETLVMK 958
               P++RPS+ ELL+  A PP++  + +   LR + ++ ++  Y +++  +F +     K
Sbjct: 910  QHDPTKRPSSQELLESEAIPPKVGEEFIQEGLRLL-SNPNTPYYLKLLKVLFGQVPDRHK 969

Query: 959  DDQLDCGTAS-----IQYTDLGTE------VRDHVIDVTRELFRLHCAKHLEISPMHLLD 1018
            D   D   +       + +D G +      VRDHV+ V    FR H AK  E   +    
Sbjct: 970  DFTYDFNLSEESGVLSKVSDRGWDSLLACLVRDHVVKV----FRRHGAKERESHILFPKS 1029

Query: 1019 GSAQINRNTVKLLSHGGDILELCHELRLPFLNWLVSCQKSSFKRYDIACVYRRAIGHSPP 1078
                 ++ +V LL   G +L+L ++  LP+   +        K Y I+ V+R A G   P
Sbjct: 1030 SQYDKDQASVSLLDKNGTLLQLPYDTVLPYARNVARNAVEEEKTYLISDVFREAKGGGRP 1089

Query: 1079 NRYLQGDFDIIGGTSALT--EAEVIKVTVDVISYF-FHSESCDIHLNHGDLLNAIWSWVG 1138
                +  FDI   +  L   +AE IK   +V++     +ESC I +NH D+L++I  ++ 
Sbjct: 1090 KAIKEISFDITTNSDNLDWYDAETIKALDEVLTEIPSLTESC-ILINHADILSSILDYLQ 1149

Query: 1139 VKAEHRHKVAELLSMMVSLRPQSSERKSKWVVIRRQLLQELKLAQAVVNRLQTVVFRFCG 1198
            V  + R     +L   ++ R   S+       +R QL  E  +    ++ L   +F F  
Sbjct: 1150 VSKDKRRMATHILG-QINQRLTLSQ-------VRNQLRIESLVPSTTLDDLS--LFDFRE 1209

Query: 1199 AVDQAVPRLRGALPTDKP--MRRALVELLDLFNYLKVWRLEANVYIDAL-MSPTEDYHRD 1258
              ++   +LR     + P  MR AL  +  +   L+  ++   +Y   L +   E Y   
Sbjct: 1210 NYEEGASKLRKIFGKEMPQKMRTALNYMERVVKLLRALKISHQLYFMPLCVYNFEFYDGG 1269

Query: 1259 IFFQVYLTKENHPGSHPEGTLVAIGGRYDYLLQQMWSRAYKTT-PPGGVGTSIALE---- 1317
            + FQ     E       +  L+  GGRYD L++       +T      VG   ALE    
Sbjct: 1270 LMFQAINLAE-------KSELICAGGRYDKLVRFFDPPLMRTARKKHVVGICFALEKLVF 1329

BLAST of CmaCh08G002010 vs. ExPASy Swiss-Prot
Match: P15442 (eIF-2-alpha kinase GCN2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=GCN2 PE=1 SV=3)

HSP 1 Score: 259.2 bits (661), Expect = 2.7e-67
Identity = 235/863 (27.23%), Postives = 379/863 (43.92%), Query Frame = 0

Query: 525  SRYLNDFEELKPLGHGGFGHVVLCKNKLDGRQYAVKKIRLKDKILPVDDRILREVSTLSR 584
            SRY +DFEE+  LG G FG VV  +N LD R YA+KKIR  ++ L     IL EV  L+ 
Sbjct: 593  SRYASDFEEIAVLGQGAFGQVVKARNALDSRYYAIKKIRHTEEKL---STILSEVMLLAS 652

Query: 585  LQHQHVVRYYQAW---------------SEAGVSDSYGEAAWGSTTPLSSTFS------- 644
            L HQ+VVRYY AW                E+ +S+S  +         SS F        
Sbjct: 653  LNHQYVVRYYAAWLEEDSMDENVFESTDEESDLSESSSDFEENDLLDQSSIFKNRTNHDL 712

Query: 645  -----------------YKGASATDA---LDHENKLES---------------------- 704
                             ++ +S  D    LDH+    S                      
Sbjct: 713  DNSNWDFISGSGYPDIVFENSSRDDENEDLDHDTSSTSSSESQDDTDKESKSIQNVPRRR 772

Query: 705  ------------TYLYIQMEYCP-RTLRQDFESYT-HFDKELAWHLFHQIVEGLAHIHGQ 764
                        + L+IQMEYC  RTL     S   +  ++  W LF QI+E L++IH Q
Sbjct: 773  NFVKPMTAVKKKSTLFIQMEYCENRTLYDLIHSENLNQQRDEYWRLFRQILEALSYIHSQ 832

Query: 765  GIIHRDLTPSNIFFDARNDIKIGDFGLAK----FLKLEQLDQDVGVPLDTTGVSIDGTGQ 824
            GIIHRDL P NIF D   ++KIGDFGLAK     L + +LD       +  G S + T  
Sbjct: 833  GIIHRDLKPMNIFIDESRNVKIGDFGLAKNVHRSLDILKLDSQ-----NLPGSSDNLTSA 892

Query: 825  VGTYFYTAPEIEQGWPKIDEKADMYSLGIVFFELWHPFGTAMERHLVLSDLKQKG-ELPT 884
            +GT  Y A E+  G    +EK DMYSLGI+FFE+ +PF T MER  +L  L+    E P 
Sbjct: 893  IGTAMYVATEVLDGTGHYNEKIDMYSLGIIFFEMIYPFSTGMERVNILKKLRSVSIEFPP 952

Query: 885  TW-VAEFPEQASLLRRLMSQSPSERPSALELLQHAF-PPRMEYQLLDNILRTMKTSEDSS 944
             +   +   +  ++R L+   P++RP A  LL   + P + + +++   L+++ ++  S 
Sbjct: 953  DFDDNKMKVEKKIIRLLIDHDPNKRPGARTLLNSGWLPVKHQDEVIKEALKSL-SNPSSP 1012

Query: 945  IYDRVVNAIFSEETLVMKDDQLDCGTASIQYTDLGTEVRDHVIDVTRELFRLHCAKHLEI 1004
               +V  ++F++   +  D   D    +   T     +R  + +   ++FR H       
Sbjct: 1013 WQQQVRESLFNQSYSLTNDILFDNSVPT--STPFANILRSQMTEEVVKIFRKHGGIENNA 1072

Query: 1005 SPMHLLDGSAQINRNTVKLLSHGGDILELCHELRLPFLNWLVSCQKSSFKRYDIACVYR- 1064
             P           +N  ++L  GG +L+L ++L  P   +L        K+Y +  VYR 
Sbjct: 1073 PPRIFPKAPIYGTQNVYEVLDKGGTVLQLQYDLTYPMARYLSKNPSLISKQYRMQHVYRP 1132

Query: 1065 --RAIGHSPPNRYLQGDFDIIGGTSA---LTEAEVIKVTVDVISYF--FHSESCDIHLNH 1124
               +     P ++ + DFDII  +S+     +AE +K+  ++++ F  F   +    LNH
Sbjct: 1133 PDHSRSSLEPRKFGEIDFDIISKSSSESGFYDAESLKIIDEILTVFPVFEKTNTFFILNH 1192

Query: 1125 GDLLNAIWSWVGVKAEHRHKVAELLSMMVSLRPQSSERKSKWVVIRRQLLQELKLAQAVV 1184
             D+L +++++  +    R  V+ +LS +   R         +  ++ +L  +L ++   +
Sbjct: 1193 ADILESVFNFTNIDKAQRPLVSRMLSQVGFAR--------SFKEVKNELKAQLNISSTAL 1252

Query: 1185 NRLQTVVFRF-CGAVDQAVPRLRGALPTDKPMRRALVELLDLFNYLKVWRLEANVYIDAL 1244
            N L+   FR    A  + + +L    P  K +  +L  +  + +YLK   +  NV I  L
Sbjct: 1253 NDLELFDFRLDFEAAKKRLYKLMIDSPHLKKIEDSLSHISKVLSYLKPLEVARNVVISPL 1312

Query: 1245 MSPTEDYHRD--IFFQVYLTKENHPGSHPEGTLVAIGGRYDYLLQQMWSRAYK--TTPPG 1268
             +    +++   +F  VY       GS     ++A GGRYD L+      + K  +    
Sbjct: 1313 SNYNSAFYKGGIMFHAVY-----DDGS--SRNMIAAGGRYDTLISFFARPSGKKSSNTRK 1372

BLAST of CmaCh08G002010 vs. ExPASy Swiss-Prot
Match: Q75JN1 (Probable serine/threonine-protein kinase ifkC OS=Dictyostelium discoideum OX=44689 GN=ifkC PE=3 SV=1)

HSP 1 Score: 257.3 bits (656), Expect = 1.0e-66
Identity = 368/1644 (22.38%), Postives = 607/1644 (36.92%), Query Frame = 0

Query: 170  SPQVTIKLRPYSNDMGFEDLDVSAFLSVKYLPGYPYKCPKLLITPEKGLAKGDTEKLLSL 229
            S +  I ++PY  D      D   F+S+  + G+P K P +L + +  + KG  +K    
Sbjct: 112  SARFRITIKPYVGD------DEKCFVSIYLVVGFPEKYPVVLPSIQVLVNKGLPQKKAIE 171

Query: 230  LHEQ--ASYNARDGRIMIFNLAEAAQEFLSEIVTIGQSNESVGCSNTDNNSQLLPDKVTS 289
            L E+       + G IMIF+L E A++FL+E             +N +    +  D    
Sbjct: 172  LEEKLIRESQGKIGNIMIFDLCEIAKDFLNE-------------NNFEPTQSIHHD---- 231

Query: 290  NEKGPYVHGYIDLFSGSGELWSWSFDMDEKPYSQAQPLAD-SLKLGTVQEKSLDKVQNLL 349
                         F    +  S     +E+   Q Q L    L+     +K  +      
Sbjct: 232  -------------FRSHQQKISIEISTNEQQQQQLQQLQQLQLQYNNDTKKEWESTLEES 291

Query: 350  TKQNSKRGELLSPSFNLGTLEEESEDE-SHSITSSHSRRSLIVQKAGDVTEDEKQDSIIV 409
              +  ++   L  S      E+E +    +  T+    +    Q+  D + D   ++   
Sbjct: 292  QLKEDRKNYRLQKSIQRAQYEKERKKTIVNEFTNGPQSKLSKQQRNIDYSIDHSINNNNN 351

Query: 410  DHAAEDDDHGSESEPSEWSFASMSKEQESQTTDRDIMMVHLLHLACAPKGPLA-DALPKL 469
            ++    ++  + +  +  + +++S         +  M++HLL L C     +  D +  L
Sbjct: 352  NNNNAFNNFNNNNNNNNTNNSNVSSIDNKTIDKQQTMILHLLRLLCQNDSNITNDHIKTL 411

Query: 470  ASELYNSGILSKVALDLASKPSSTFHKKFESAFQE----EMNATSFSE------------ 529
              +L   GI ++  L L +  +S  ++ ++  FQ+    + N  S S+            
Sbjct: 412  GEQLVQMGIFNRNHLSLLNMHNSNDNQIYQHIFQDYFLKQFNDISLSDLKNNVDSAANNS 471

Query: 530  ------FW--------------NPSDF-----------GGPTSSQL-----SSRYLNDFE 589
                  FW              +PS F           GG +S +       SRY +DFE
Sbjct: 472  GHFLNKFWDTLNKTNENSGLKKSPSTFEYSGEGGGGGVGGGSSQKTINPHQQSRYHSDFE 531

Query: 590  ELKPLGHGGFGHVVLCKNKLDGRQYAVKKIRLKDKILPVDDRILREVSTLSRLQHQHVVR 649
            E++ LG GGFG VV  +NKLDGR YA+KKI+L D    ++ RILREV TLSRL HQHVVR
Sbjct: 532  EIQLLGRGGFGQVVKVRNKLDGRYYAIKKIKL-DSNQSLNRRILREVITLSRLHHQHVVR 591

Query: 650  YYQAWSEAGVS------------DSYGEAAWGSTTPLSSTFSYKGASAT----------- 709
            YYQAW E+  S            D   E   GS +  S + S   +S +           
Sbjct: 592  YYQAWIESAESLSLTNDNSDDDDDDDDEFESGSESEESQSESESESSESEESSESEESSE 651

Query: 710  ------------------------------------------------------------ 769
                                                                        
Sbjct: 652  SSESEESEESEYSDDSEFESEENNDFDQSFSEVFLFQNGNKRSLEFDLTDDSYSFLHSDC 711

Query: 770  ----DALDHENKL----------------------------------------------- 829
                +  D  NK+                                               
Sbjct: 712  GFLLEVFDLNNKIGGNNTRSMGSSNQHLQQQQQQNQSQQQKKQPQQNQSQQQKKLKNSNS 771

Query: 830  -------------------------------EST---YLYIQMEYCPRTLRQDFESYTHF 889
                                           +ST   YLYIQMEYC + LR   E+  + 
Sbjct: 772  KSKSKSKSKSKSKSKSNSKKSISSSKLKNIKKSTSIPYLYIQMEYCQKILRNLTETGMNL 831

Query: 890  DKELAWHLFHQIVEGLAHIHGQGIIHRDLTPSNIFFDARNDIKIGDFGLAKFLK-LEQLD 949
            + +  W LF QIVEG+A++HGQGIIHRDL PSNIFFD+  DIKIGDFGLA   K    L 
Sbjct: 832  EDDDIWKLFRQIVEGMAYVHGQGIIHRDLKPSNIFFDSCGDIKIGDFGLAINNKTTSTLS 891

Query: 950  QDVGVPLDTTGV-------------------------------SIDG------------- 1009
                +   T+                                  ++G             
Sbjct: 892  PTTNINSSTSSAGSLTTSSNSVQSVINTKQQQQQQQPQNYWEDEVEGQQQQQQQQQQQQQ 951

Query: 1010 ------------TGQVGTYFYTAPEIEQGW---PKIDEKADMYSLGIVFFELWHPFGTAM 1069
                        T +VGT FYT+PE E G       D+K DMYSLGIVFFE+W+ F T  
Sbjct: 952  QQQQQQQQQQQHTARVGTLFYTSPEQEAGTNGDSAYDDKVDMYSLGIVFFEMWYVFSTGH 1011

Query: 1070 ERHLVLSDLKQKGELPTTWVAEFPEQASLLRRLMSQSPSERPSALELLQ-HAFPPRMEYQ 1129
            ER +VL +L++K E P+ +      QA+L+R L+ + P++RPSA +LLQ    PP+ME +
Sbjct: 1012 ERVIVLRNLREKFEFPSDFERNHSRQATLIRMLIDKDPAKRPSAQQLLQSELMPPKMEDE 1071

Query: 1130 LLDNILRTMKTSEDSSIYDRVVNAIFSE-----ETLVMKDDQLDCGTASIQYT-----DL 1189
             + N +R + T+  +  Y  ++N++FS+      + +    Q    ++S   +     DL
Sbjct: 1072 YIKNSIRVI-TNPTNQFYQTMLNSLFSQNHQHLHSHIYHHQQSSVTSSSYSKSLFCSLDL 1131

Query: 1190 GTEVRDHVIDVTRELFRLHCAKHLEISPMHLL---------------------------- 1249
              +V+  V DV   + + H A  +    M ++                            
Sbjct: 1132 -LQVKQLVDDVIITILKKHNANEMLTPQMSIIKEWNEQPNLFNNSSSSSSTTTTTSTTNT 1191

Query: 1250 ---DGSAQINRNTVK--------------LLSHGGDILELCHELRLPFLNWLVS------ 1309
                 S  IN++T                L+   G +LEL H+LR+ F +++ +      
Sbjct: 1192 ANNTNSVVINKSTSTSNINKVSNKTGKSILMDDSGQLLELRHDLRVSFKSFIETEFLNLG 1251

Query: 1310 ---------------------------------------------------CQKSSFKRY 1334
                                                                 K+  KRY
Sbjct: 1252 DHYKHHHQQQNDVRHDNSSNGNSNNNNSNDRHHDQDKSNTTTRSVDEILELLSKAPIKRY 1311

BLAST of CmaCh08G002010 vs. ExPASy Swiss-Prot
Match: Q558U1 (Probable serine/threonine-protein kinase ifkA OS=Dictyostelium discoideum OX=44689 GN=ifkA PE=2 SV=1)

HSP 1 Score: 243.0 bits (619), Expect = 2.0e-62
Identity = 204/771 (26.46%), Postives = 346/771 (44.88%), Query Frame = 0

Query: 639  ESTYLYIQMEYC-PRTLRQDFESYTHFDKELAWHLFHQIVEGLAHIHGQGIIHRDLTPSN 698
            E+  LYIQMEYC  +TL+   ++     +E A+ L  QIVEGL HIH Q IIHRDL P+N
Sbjct: 1259 ETHTLYIQMEYCSKKTLKTLIDNVGGIAEEEAFRLLRQIVEGLNHIHSQQIIHRDLKPAN 1318

Query: 699  IFFDARNDIKIGDFGL----AKFLKLEQLDQDVG------------------VPL-DTTG 758
            IF D   ++KIGDFGL    A   K + L+                       P+ D   
Sbjct: 1319 IFIDNEQNVKIGDFGLATSGAPVSKSDDLNSSTSNTANNINLSSSTNSTAQQTPMWDLND 1378

Query: 759  VSIDGTGQVGTYFYTAPEI-EQGWPKIDEKADMYSLGIVFFELWHPFGTAMERHLVLSDL 818
             ++  TG VGT FY  PEI E+       K DMYSLGI+FFE+   F T MER  +L DL
Sbjct: 1379 ENLSMTGGVGTPFYCCPEILEKNTKHYGTKVDMYSLGIIFFEMCFQFQTQMERSNILRDL 1438

Query: 819  KQKGELPTTWVAEFPEQASLLRRLMSQSPSERPSALELLQHA-FPPRMEYQLLDNILRTM 878
            +   + P  + +  P+Q  ++R L+S+ P++RPS  +LL+    P +ME  +L   ++T+
Sbjct: 1439 RDNLKFPPGFESTKPDQTQIIRSLLSRDPTQRPSTKQLLESGLLPSKMEDDILKEAIKTI 1498

Query: 879  KTSEDSSIYDRVVNAIF--SEETLVMKDDQLDCGTASIQYTDLGTEVRDHVIDVTRELFR 938
              +   S++  ++  +F  S +  +M        T++   T +    R+       ++F 
Sbjct: 1499 -ANPTISLFSYLMEKLFCLSNDEHIMSRYLY---TSNPSLTPMHLICREKTFSRLEKIFL 1558

Query: 939  LHCAKHLE---ISPMHLLDGSAQINRNTVKLLSHGGDILELCHELRLPFLNWLVSCQKSS 998
             H +  ++   + P +  + +     N  K L   G ++ L ++L +P+   +V      
Sbjct: 1559 NHGSIRIDTPTLFPKNPTNSTHPGAANVAKFLDESGTVVYLPYDLTIPWARHVVIHNIQQ 1618

Query: 999  FKRYDIACVYRRAIGHSPPNRYLQGDFDIIGGTSA--LTEAEVIKVTVDVISYFFH---- 1058
             KRY  + VYRR+     P    + DFDIIG + +  +++AE +++ ++++  F      
Sbjct: 1619 AKRYTFSKVYRRSQPGFSPKELYECDFDIIGPSKSRHISDAETLRIIIEIMEEFKDELFG 1678

Query: 1059 -------------SESCDIHLNHGDLLNAIWSWVGVKAEHRHKVAELLSMMVSLRPQSSE 1118
                         S +  I +NH  LL++I S  GV+      V + ++ +         
Sbjct: 1679 NNSGSNSGSGSGGSINYKIRINHYGLLDSILSECGVEKRFFTVVYQTVAQL--------H 1738

Query: 1119 RKSKWVVIRRQLLQELKLAQAVVNRLQTVVFRFCGAVDQAVPRLRGALPTDKPMRRALVE 1178
             +  W  +  Q L+E  L+ ++V+ + T  FR  G + Q V +L   L   K     + +
Sbjct: 1739 WRLNWAQV-AQSLKEHGLSASIVSNIST-YFRQRGELAQCVTQLESLLANHKEATTGISD 1798

Query: 1179 LLDLFNYLKVWRLEANVYIDALMSPTEDYHRDIFFQVYLTKENHPGSHPEGT-LVAIGGR 1238
            L  L   L++  +     +D  +     Y+  + FQ Y+  E    S+P  T ++  GGR
Sbjct: 1799 LKILVRNLQMINIIPRFLLDLSLIHNHQYYEGLVFQAYI--ERPTLSNPSRTEIIMSGGR 1858

Query: 1239 YDYLLQQMW-SRAYKTTPPGGVGTSIALETMIQQAAVDLKPIRN---------------- 1298
            YD L++ +  + +       GVG ++A E ++       + + N                
Sbjct: 1859 YDKLIKSLHPNPSLSNNIVSGVGVTLACEKIVNSVLNYRQHLLNNCFNTTYTRRNKDPPN 1918

Query: 1299 --------------------EGSTSFLVCSRGGGGLLLERMELVVELWEEKIKAQLVPTP 1322
                                +      VCS  G  LL E++++  +LW   IKA    T 
Sbjct: 1919 SNNNPNNNNLNNNVSIFTKFQSHIEVFVCSL-GSSLLGEKLQVASQLWSAGIKADYSQTD 1978

HSP 2 Score: 90.5 bits (223), Expect = 1.7e-16
Identity = 46/79 (58.23%), Postives = 57/79 (72.15%), Query Frame = 0

Query: 525 SRYLNDFEELKPLGHGGFGHVVLCKNKLDGRQYAVKKIRLK----DKILPVDDRILREVS 584
           SRY  DFEE++ +G GGFG VV  +NKLD R YA+KKI+ K        P+ +++LREV+
Sbjct: 888 SRYRTDFEEIEMIGKGGFGVVVKSRNKLDCRYYAIKKIKTKGYTDSNQEPLTNKLLREVT 947

Query: 585 TLSRLQHQHVVRYYQAWSE 600
           TLSRL HQ VVRYYQAW E
Sbjct: 948 TLSRLHHQFVVRYYQAWIE 966

BLAST of CmaCh08G002010 vs. TAIR 10
Match: AT3G59410.1 (protein kinase family protein )

HSP 1 Score: 1506.9 bits (3900), Expect = 0.0e+00
Identity = 781/1238 (63.09%), Postives = 942/1238 (76.09%), Query Frame = 0

Query: 114  SSKKKRRGGGGGGKRSKGRTPQKDH-SFSGEEGELISEELTALCGIFQEDCKVVSG--PS 173
            SS KK++  GG G+R +     KDH S + E+ EL+SEE+TAL  IFQEDCKVVS     
Sbjct: 4    SSSKKKKKRGGSGRRGQ----LKDHGSNADEDNELLSEEITALSAIFQEDCKVVSDSRSP 63

Query: 174  PQVTIKLRPYSNDMGFEDLDVSAFLSVKYLPGYPYKCPKLLITPEKGLAKGDTEKLLSLL 233
            PQ+ IKLRPYS DMG+ED D+SA L V+ LPGYPYKCPKL ITPE+GL   D EKLLSLL
Sbjct: 64   PQIAIKLRPYSKDMGYEDTDISAMLIVRCLPGYPYKCPKLQITPEQGLTTADAEKLLSLL 123

Query: 234  HEQASYNARDGRIMIFNLAEAAQEFLSEIVTIGQSNESVGCSNTDNNSQLLPDKVTSN-- 293
             +QA+ NAR+GR+MIFNL EAAQEFLSEI+      ESV C     ++Q +   + SN  
Sbjct: 124  EDQANSNAREGRVMIFNLVEAAQEFLSEIIPESHDEESVPCLTAHRSTQFIEQPMLSNIA 183

Query: 294  ---EKGPYVHGYIDLFSGSGELWSWSFDMDEK----PYSQAQPLADSLKLGTVQEKSLDK 353
                 GP+V+G+IDLFSG  +  +WS   DE        Q+ PL  S  L    +K+L +
Sbjct: 184  KSCSGGPFVYGFIDLFSGLEDARNWSLTPDENRGIVSSVQSHPLDTSRILHQKPDKNLKR 243

Query: 354  VQNLLTKQNSKRGELLSPSFNLGTLEEESEDESHSITSSHSRRSLIVQKAGDVTEDEKQD 413
             ++   ++ +    L +P   L T++EE+ D++ SI+S  S +S    ++G + ++EK++
Sbjct: 244  FEDHAKEEVA----LPAPIAKLNTVQEENVDDT-SISSFDSSKSTDDVESG-LFQNEKKE 303

Query: 414  SIIVDHAAEDDDHGSESEP-SEWSFASMSKEQESQTTDRDIMMVHLLHLACAPKGPLADA 473
            S + D  AEDD   SESE    WS  S++++Q  Q + +D++MVHLL +AC  +GPLADA
Sbjct: 304  SNLQDDTAEDDSTNSESESLGSWSSDSLAQDQVPQISKKDLLMVHLLRVACTSRGPLADA 363

Query: 474  LPKLASELYNSGILSKVALDLASKPSSTFHKKFESAFQEEMNATSFSEFWN-PSDFGGPT 533
            LP++  EL+  GILS+  LDLASK S  F++ FE AF + M +TS  +FW  PSD   P 
Sbjct: 364  LPQITDELHELGILSEEVLDLASKSSPDFNRTFEHAFNQNMASTSVPQFWEPPSDSCEPN 423

Query: 534  SSQLSSRYLNDFEELKPLGHGGFGHVVLCKNKLDGRQYAVKKIRLKDKILPVDDRILREV 593
            +S  SSRYLNDFEELKPLG GGFGHVVLCKNKLDGRQYAVKKIRLKDK +PV+ RI+REV
Sbjct: 424  ASLPSSRYLNDFEELKPLGQGGFGHVVLCKNKLDGRQYAVKKIRLKDKEIPVNSRIVREV 483

Query: 594  STLSRLQHQHVVRYYQAWSEAGVSDSYGEAAWGSTTPLSSTFSYKGASATDALDHENKLE 653
            +TLSRLQHQHVVRYYQAW E GV D +  A WGS T  SS FSY GA +T+  + +N LE
Sbjct: 484  ATLSRLQHQHVVRYYQAWFETGVVDPFAGANWGSKTAGSSMFSYSGAVSTEIPEQDNNLE 543

Query: 654  STYLYIQMEYCPRTLRQDFESYTHFDKELAWHLFHQIVEGLAHIHGQGIIHRDLTPSNIF 713
            STYLYIQMEYCPRTLRQ FESY HFDK+ AWHL  QIVEGLAHIHGQGIIHRD TP+NIF
Sbjct: 544  STYLYIQMEYCPRTLRQVFESYNHFDKDFAWHLIRQIVEGLAHIHGQGIIHRDFTPNNIF 603

Query: 714  FDARNDIKIGDFGLAKFLKLEQLDQDVGVPLDTTGVSIDGTGQVGTYFYTAPEIEQGWPK 773
            FDARNDIKIGDFGLAKFLKLEQLDQD G   D  G  +D TGQ GTYFYTAPEIEQ WPK
Sbjct: 604  FDARNDIKIGDFGLAKFLKLEQLDQDGGFSTDVAGSGVDSTGQAGTYFYTAPEIEQDWPK 663

Query: 774  IDEKADMYSLGIVFFELWHPFGTAMERHLVLSDLKQKGELPTTWVAEFPEQASLLRRLMS 833
            IDEKADMYSLG+VFFELWHPFGTAMERH++L++LK KGELP  WV EFPEQASLLRRLMS
Sbjct: 664  IDEKADMYSLGVVFFELWHPFGTAMERHVILTNLKLKGELPLKWVNEFPEQASLLRRLMS 723

Query: 834  QSPSERPSALELLQHAFPPRMEYQLLDNILRTMKTSEDSSIYDRVVNAIFSEETLVMKDD 893
             SPS+RPSA ELL+HAFPPRME +LLDNILR M+TSEDSS+YDRVV+ IF EE L MK  
Sbjct: 724  PSPSDRPSATELLKHAFPPRMESELLDNILRIMQTSEDSSVYDRVVSVIFDEEVLEMKSH 783

Query: 894  Q-----LDCGTASIQYTDLGTEVRDHVIDVTRELFRLHCAKHLEISPMHLLDGSAQINRN 953
            Q     L    + IQYT++ TE+RD+V+++T+E+FR HCAKHLE+ PM LL    Q +R 
Sbjct: 784  QSSRSRLCADDSYIQYTEINTELRDYVVEITKEVFRQHCAKHLEVIPMRLLSDCPQFSRK 843

Query: 954  TVKLLSHGGDILELCHELRLPFLNWLVSCQKSSFKRYDIACVYRRAIGHSPPNRYLQGDF 1013
            TVKLL++GGD+LELC+ELRLPF++W+   QKSSFKRY+I+ VYRRAIGHSPPN  LQ DF
Sbjct: 844  TVKLLTNGGDMLELCYELRLPFVHWISVNQKSSFKRYEISHVYRRAIGHSPPNPCLQADF 903

Query: 1014 DIIGGTSALTEAEVIKVTVDVISYFFHSESCDIHLNHGDLLNAIWSWVGVKAEHRHKVAE 1073
            DI+GGT +LTEAEV+KV VD+ ++ FH  SCDIHLNHGDLL+AIWSW G+KAEHR KVAE
Sbjct: 904  DIVGGTLSLTEAEVLKVIVDITTHIFHRGSCDIHLNHGDLLDAIWSWAGIKAEHRRKVAE 963

Query: 1074 LLSMMVSLRPQSSERKSKWVVIRRQLLQELKLAQAVVNRLQTVVFRFCGAVDQAVPRLRG 1133
            LLSMM SLRPQSSERK KWV IRRQLLQELKL +AVVNRLQTV  RFCG  DQA+PRLRG
Sbjct: 964  LLSMMGSLRPQSSERKLKWVFIRRQLLQELKLPEAVVNRLQTVASRFCGDADQALPRLRG 1023

Query: 1134 ALPTDKPMRRALVELLDLFNYLKVWRLEANVYIDALMSPTEDYHRDIFFQVYLTKENHPG 1193
            AL  D+P R+AL EL +L  YL+VWR+E +V+ID LM PTE YHR++FFQV+LTKEN  G
Sbjct: 1024 ALRADRPTRKALDELSNLLTYLRVWRIEEHVHIDVLMPPTESYHRNLFFQVFLTKENSSG 1083

Query: 1194 SHPEGTLVAIGGRYDYLLQQMWSRAYKTTPPGGVGTSIALETMIQQAAVDLKPIRNEGST 1253
            +  +G L+A+GGRYD+L+Q++  R +K   PG VG S+ALET+ Q   +DL+PIRNE ST
Sbjct: 1084 TSNDGVLLAVGGRYDWLVQEVCDREHKMNLPGAVGVSLALETIFQHLPMDLRPIRNEVST 1143

Query: 1254 SFLVCSRGGGGLLLERMELVVELWEEKIKAQLVPTPDPSFTEQYEYANEHDIKCLVIITD 1313
            S LVCSRGGGGLL++RMELV ELWE+ IKA+ VPTPDPS TEQYEYANEH+IKCLVIIT+
Sbjct: 1144 SVLVCSRGGGGLLVQRMELVAELWEKSIKAEFVPTPDPSLTEQYEYANEHEIKCLVIITE 1203

Query: 1314 SGVSNR--GSVRVRHLELKREKEVERTSICFSSLNYLA 1331
            SGV+      V+VRHLELK+EK V R  +    L+ +A
Sbjct: 1204 SGVAQNQIEFVKVRHLELKKEKVVGREELVKFLLDAMA 1231

BLAST of CmaCh08G002010 vs. TAIR 10
Match: AT3G59410.2 (protein kinase family protein )

HSP 1 Score: 1493.4 bits (3865), Expect = 0.0e+00
Identity = 781/1262 (61.89%), Postives = 942/1262 (74.64%), Query Frame = 0

Query: 114  SSKKKRRGGGGGGKRSKGRTPQKDH-SFSGEEGELISEELTAL----------------- 173
            SS KK++  GG G+R +     KDH S + E+ EL+SEE+TAL                 
Sbjct: 4    SSSKKKKKRGGSGRRGQ----LKDHGSNADEDNELLSEEITALNCVNVGIMGFDRSDVTG 63

Query: 174  -------CGIFQEDCKVVSG--PSPQVTIKLRPYSNDMGFEDLDVSAFLSVKYLPGYPYK 233
                     IFQEDCKVVS     PQ+ IKLRPYS DMG+ED D+SA L V+ LPGYPYK
Sbjct: 64   KNNSNYFSAIFQEDCKVVSDSRSPPQIAIKLRPYSKDMGYEDTDISAMLIVRCLPGYPYK 123

Query: 234  CPKLLITPEKGLAKGDTEKLLSLLHEQASYNARDGRIMIFNLAEAAQEFLSEIVTIGQSN 293
            CPKL ITPE+GL   D EKLLSLL +QA+ NAR+GR+MIFNL EAAQEFLSEI+      
Sbjct: 124  CPKLQITPEQGLTTADAEKLLSLLEDQANSNAREGRVMIFNLVEAAQEFLSEIIPESHDE 183

Query: 294  ESVGCSNTDNNSQLLPDKVTSN-----EKGPYVHGYIDLFSGSGELWSWSFDMDEK---- 353
            ESV C     ++Q +   + SN       GP+V+G+IDLFSG  +  +WS   DE     
Sbjct: 184  ESVPCLTAHRSTQFIEQPMLSNIAKSCSGGPFVYGFIDLFSGLEDARNWSLTPDENRGIV 243

Query: 354  PYSQAQPLADSLKLGTVQEKSLDKVQNLLTKQNSKRGELLSPSFNLGTLEEESEDESHSI 413
               Q+ PL  S  L    +K+L + ++   ++ +    L +P   L T++EE+ D++ SI
Sbjct: 244  SSVQSHPLDTSRILHQKPDKNLKRFEDHAKEEVA----LPAPIAKLNTVQEENVDDT-SI 303

Query: 414  TSSHSRRSLIVQKAGDVTEDEKQDSIIVDHAAEDDDHGSESEP-SEWSFASMSKEQESQT 473
            +S  S +S    ++G + ++EK++S + D  AEDD   SESE    WS  S++++Q  Q 
Sbjct: 304  SSFDSSKSTDDVESG-LFQNEKKESNLQDDTAEDDSTNSESESLGSWSSDSLAQDQVPQI 363

Query: 474  TDRDIMMVHLLHLACAPKGPLADALPKLASELYNSGILSKVALDLASKPSSTFHKKFESA 533
            + +D++MVHLL +AC  +GPLADALP++  EL+  GILS+  LDLASK S  F++ FE A
Sbjct: 364  SKKDLLMVHLLRVACTSRGPLADALPQITDELHELGILSEEVLDLASKSSPDFNRTFEHA 423

Query: 534  FQEEMNATSFSEFWN-PSDFGGPTSSQLSSRYLNDFEELKPLGHGGFGHVVLCKNKLDGR 593
            F + M +TS  +FW  PSD   P +S  SSRYLNDFEELKPLG GGFGHVVLCKNKLDGR
Sbjct: 424  FNQNMASTSVPQFWEPPSDSCEPNASLPSSRYLNDFEELKPLGQGGFGHVVLCKNKLDGR 483

Query: 594  QYAVKKIRLKDKILPVDDRILREVSTLSRLQHQHVVRYYQAWSEAGVSDSYGEAAWGSTT 653
            QYAVKKIRLKDK +PV+ RI+REV+TLSRLQHQHVVRYYQAW E GV D +  A WGS T
Sbjct: 484  QYAVKKIRLKDKEIPVNSRIVREVATLSRLQHQHVVRYYQAWFETGVVDPFAGANWGSKT 543

Query: 654  PLSSTFSYKGASATDALDHENKLESTYLYIQMEYCPRTLRQDFESYTHFDKELAWHLFHQ 713
              SS FSY GA +T+  + +N LESTYLYIQMEYCPRTLRQ FESY HFDK+ AWHL  Q
Sbjct: 544  AGSSMFSYSGAVSTEIPEQDNNLESTYLYIQMEYCPRTLRQVFESYNHFDKDFAWHLIRQ 603

Query: 714  IVEGLAHIHGQGIIHRDLTPSNIFFDARNDIKIGDFGLAKFLKLEQLDQDVGVPLDTTGV 773
            IVEGLAHIHGQGIIHRD TP+NIFFDARNDIKIGDFGLAKFLKLEQLDQD G   D  G 
Sbjct: 604  IVEGLAHIHGQGIIHRDFTPNNIFFDARNDIKIGDFGLAKFLKLEQLDQDGGFSTDVAGS 663

Query: 774  SIDGTGQVGTYFYTAPEIEQGWPKIDEKADMYSLGIVFFELWHPFGTAMERHLVLSDLKQ 833
             +D TGQ GTYFYTAPEIEQ WPKIDEKADMYSLG+VFFELWHPFGTAMERH++L++LK 
Sbjct: 664  GVDSTGQAGTYFYTAPEIEQDWPKIDEKADMYSLGVVFFELWHPFGTAMERHVILTNLKL 723

Query: 834  KGELPTTWVAEFPEQASLLRRLMSQSPSERPSALELLQHAFPPRMEYQLLDNILRTMKTS 893
            KGELP  WV EFPEQASLLRRLMS SPS+RPSA ELL+HAFPPRME +LLDNILR M+TS
Sbjct: 724  KGELPLKWVNEFPEQASLLRRLMSPSPSDRPSATELLKHAFPPRMESELLDNILRIMQTS 783

Query: 894  EDSSIYDRVVNAIFSEETLVMKDDQ-----LDCGTASIQYTDLGTEVRDHVIDVTRELFR 953
            EDSS+YDRVV+ IF EE L MK  Q     L    + IQYT++ TE+RD+V+++T+E+FR
Sbjct: 784  EDSSVYDRVVSVIFDEEVLEMKSHQSSRSRLCADDSYIQYTEINTELRDYVVEITKEVFR 843

Query: 954  LHCAKHLEISPMHLLDGSAQINRNTVKLLSHGGDILELCHELRLPFLNWLVSCQKSSFKR 1013
             HCAKHLE+ PM LL    Q +R TVKLL++GGD+LELC+ELRLPF++W+   QKSSFKR
Sbjct: 844  QHCAKHLEVIPMRLLSDCPQFSRKTVKLLTNGGDMLELCYELRLPFVHWISVNQKSSFKR 903

Query: 1014 YDIACVYRRAIGHSPPNRYLQGDFDIIGGTSALTEAEVIKVTVDVISYFFHSESCDIHLN 1073
            Y+I+ VYRRAIGHSPPN  LQ DFDI+GGT +LTEAEV+KV VD+ ++ FH  SCDIHLN
Sbjct: 904  YEISHVYRRAIGHSPPNPCLQADFDIVGGTLSLTEAEVLKVIVDITTHIFHRGSCDIHLN 963

Query: 1074 HGDLLNAIWSWVGVKAEHRHKVAELLSMMVSLRPQSSERKSKWVVIRRQLLQELKLAQAV 1133
            HGDLL+AIWSW G+KAEHR KVAELLSMM SLRPQSSERK KWV IRRQLLQELKL +AV
Sbjct: 964  HGDLLDAIWSWAGIKAEHRRKVAELLSMMGSLRPQSSERKLKWVFIRRQLLQELKLPEAV 1023

Query: 1134 VNRLQTVVFRFCGAVDQAVPRLRGALPTDKPMRRALVELLDLFNYLKVWRLEANVYIDAL 1193
            VNRLQTV  RFCG  DQA+PRLRGAL  D+P R+AL EL +L  YL+VWR+E +V+ID L
Sbjct: 1024 VNRLQTVASRFCGDADQALPRLRGALRADRPTRKALDELSNLLTYLRVWRIEEHVHIDVL 1083

Query: 1194 MSPTEDYHRDIFFQVYLTKENHPGSHPEGTLVAIGGRYDYLLQQMWSRAYKTTPPGGVGT 1253
            M PTE YHR++FFQV+LTKEN  G+  +G L+A+GGRYD+L+Q++  R +K   PG VG 
Sbjct: 1084 MPPTESYHRNLFFQVFLTKENSSGTSNDGVLLAVGGRYDWLVQEVCDREHKMNLPGAVGV 1143

Query: 1254 SIALETMIQQAAVDLKPIRNEGSTSFLVCSRGGGGLLLERMELVVELWEEKIKAQLVPTP 1313
            S+ALET+ Q   +DL+PIRNE STS LVCSRGGGGLL++RMELV ELWE+ IKA+ VPTP
Sbjct: 1144 SLALETIFQHLPMDLRPIRNEVSTSVLVCSRGGGGLLVQRMELVAELWEKSIKAEFVPTP 1203

Query: 1314 DPSFTEQYEYANEHDIKCLVIITDSGVSNR--GSVRVRHLELKREKEVERTSICFSSLNY 1331
            DPS TEQYEYANEH+IKCLVIIT+SGV+      V+VRHLELK+EK V R  +    L+ 
Sbjct: 1204 DPSLTEQYEYANEHEIKCLVIITESGVAQNQIEFVKVRHLELKKEKVVGREELVKFLLDA 1255

BLAST of CmaCh08G002010 vs. TAIR 10
Match: AT3G44200.1 (NIMA (never in mitosis, gene A)-related 6 )

HSP 1 Score: 108.6 bits (270), Expect = 4.2e-23
Identity = 80/316 (25.32%), Postives = 142/316 (44.94%), Query Frame = 0

Query: 528 LNDFEELKPLGHGGFGHVVLCKNKLDGRQYAVKKIRLKDKILPVDDRILREVSTLSRLQH 587
           ++ +E ++ +G G FG  +L  +K + ++Y +KKIRL  +         +E+S ++R+QH
Sbjct: 5   MDQYELMEQIGRGAFGAAILVHHKAERKKYVLKKIRLARQTERCRRSAHQEMSLIARVQH 64

Query: 588 QHVVRYYQAWSEAGVSDSYGEAAWGSTTPLSSTFSYKGASATDALDHENKLESTYLYIQM 647
            ++V + +AW E G                                        Y+ I  
Sbjct: 65  PYIVEFKEAWVEKG---------------------------------------CYVCIVT 124

Query: 648 EYCPRTLRQDF---ESYTHFDKELAWHLFHQIVEGLAHIHGQGIIHRDLTPSNIFFDARN 707
            YC      +     +  +F +E     F Q++  + ++H   ++HRDL  SNIF     
Sbjct: 125 GYCEGGDMAELMKKSNGVYFPEEKLCKWFTQLLLAVEYLHSNYVLHRDLKCSNIFLTKDQ 184

Query: 708 DIKIGDFGLAKFLKLEQLDQDVGVPLDTTGVSIDGTGQVGTYFYTAPEIEQGWPKIDEKA 767
           D+++GDFGLAK LK + L   V                VGT  Y  PE+    P    K+
Sbjct: 185 DVRLGDFGLAKTLKADDLTSSV----------------VGTPNYMCPELLADIP-YGFKS 244

Query: 768 DMYSLGIVFFEL--WHPFGTAMERHLVLSDLKQK--GELPTTWVAEFPEQASLLRRLMSQ 827
           D++SLG   +E+  + P   A +   ++S + +   G LP  +    P   +L++ ++ +
Sbjct: 245 DIWSLGCCIYEMAAYRPAFKAFDMAGLISKVNRSSIGPLPPCY---SPSLKALIKGMLRK 261

Query: 828 SPSERPSALELLQHAF 837
           +P  RP+A E+L+H +
Sbjct: 305 NPEYRPNASEILKHPY 261

BLAST of CmaCh08G002010 vs. TAIR 10
Match: AT1G54510.1 (NIMA-related serine/threonine kinase 1 )

HSP 1 Score: 108.2 bits (269), Expect = 5.5e-23
Identity = 84/332 (25.30%), Postives = 148/332 (44.58%), Query Frame = 0

Query: 528 LNDFEELKPLGHGGFGHVVLCKNKLDGRQYAVKKIRLKDKILPVDDRILREVSTLSRLQH 587
           +  +E L+ +G G FG  +L ++K + ++Y +KKIRL  +         +E+  +S+++H
Sbjct: 1   MEQYEFLEQIGKGSFGSALLVRHKHEKKKYVLKKIRLARQTQRTRRSAHQEMELISKMRH 60

Query: 588 QHVVRYYQAWSEAGVSDSYGEAAWGSTTPLSSTFSYKGASATDALDHENKLESTYLYIQM 647
             +V Y  +W E                                       ++ Y+ I +
Sbjct: 61  PFIVEYKDSWVE---------------------------------------KACYVCIVI 120

Query: 648 EYC-----PRTLRQDFESYTHFDKELAWHLFHQIVEGLAHIHGQGIIHRDLTPSNIFFDA 707
            YC      + +++   +  HF +E       Q++ GL ++H   I+HRD+  SNIF   
Sbjct: 121 GYCEGGDMAQAIKK--SNGVHFQEEKLCKWLVQLLMGLEYLHSNHILHRDVKCSNIFLTK 180

Query: 708 RNDIKIGDFGLAKFLKLEQLDQDVGVPLDTTGVSIDGTGQVGTYFYTAPEIEQGWPKIDE 767
             DI++GDFGLAK L  + L   V                VGT  Y  PE+    P    
Sbjct: 181 EQDIRLGDFGLAKILTSDDLTSSV----------------VGTPSYMCPELLADIP-YGS 240

Query: 768 KADMYSLGIVFFELWH--PFGTAMERHLVLSDLKQK--GELPTTWVAEFPEQASLLRRLM 827
           K+D++SLG   +E+ +  P   A +   +++ + +     LP  +   F     L++ ++
Sbjct: 241 KSDIWSLGCCIYEMAYLKPAFKAFDMQALINKINKTIVSPLPAKYSGPF---RGLVKSML 268

Query: 828 SQSPSERPSALELLQHAFPPRMEYQLLDNILR 851
            ++P  RPSA +LL+H   P ++  +LD  LR
Sbjct: 301 RKNPEVRPSASDLLRH---PHLQPYVLDVKLR 268

BLAST of CmaCh08G002010 vs. TAIR 10
Match: AT1G54510.2 (NIMA-related serine/threonine kinase 1 )

HSP 1 Score: 108.2 bits (269), Expect = 5.5e-23
Identity = 84/332 (25.30%), Postives = 148/332 (44.58%), Query Frame = 0

Query: 528 LNDFEELKPLGHGGFGHVVLCKNKLDGRQYAVKKIRLKDKILPVDDRILREVSTLSRLQH 587
           +  +E L+ +G G FG  +L ++K + ++Y +KKIRL  +         +E+  +S+++H
Sbjct: 1   MEQYEFLEQIGKGSFGSALLVRHKHEKKKYVLKKIRLARQTQRTRRSAHQEMELISKMRH 60

Query: 588 QHVVRYYQAWSEAGVSDSYGEAAWGSTTPLSSTFSYKGASATDALDHENKLESTYLYIQM 647
             +V Y  +W E                                       ++ Y+ I +
Sbjct: 61  PFIVEYKDSWVE---------------------------------------KACYVCIVI 120

Query: 648 EYC-----PRTLRQDFESYTHFDKELAWHLFHQIVEGLAHIHGQGIIHRDLTPSNIFFDA 707
            YC      + +++   +  HF +E       Q++ GL ++H   I+HRD+  SNIF   
Sbjct: 121 GYCEGGDMAQAIKK--SNGVHFQEEKLCKWLVQLLMGLEYLHSNHILHRDVKCSNIFLTK 180

Query: 708 RNDIKIGDFGLAKFLKLEQLDQDVGVPLDTTGVSIDGTGQVGTYFYTAPEIEQGWPKIDE 767
             DI++GDFGLAK L  + L   V                VGT  Y  PE+    P    
Sbjct: 181 EQDIRLGDFGLAKILTSDDLTSSV----------------VGTPSYMCPELLADIP-YGS 240

Query: 768 KADMYSLGIVFFELWH--PFGTAMERHLVLSDLKQK--GELPTTWVAEFPEQASLLRRLM 827
           K+D++SLG   +E+ +  P   A +   +++ + +     LP  +   F     L++ ++
Sbjct: 241 KSDIWSLGCCIYEMAYLKPAFKAFDMQALINKINKTIVSPLPAKYSGPF---RGLVKSML 268

Query: 828 SQSPSERPSALELLQHAFPPRMEYQLLDNILR 851
            ++P  RPSA +LL+H   P ++  +LD  LR
Sbjct: 301 RKNPEVRPSASDLLRH---PHLQPYVLDVKLR 268