CmaCh07G012680 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh07G012680
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Descriptionamino acid permease 4-like
LocationCma_Chr07: 7025627 .. 7029350 (-)
RNA-Seq ExpressionCmaCh07G012680
SyntenyCmaCh07G012680
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ACTCCCCCTAGCTCCATCACCTTTCTCCCTTTCCCCTCAACCTTTCCAACCCTACTCCGTCGTCATGGCCGTGCTCCCAATCAACGACTCCCCGAGCTTCGATGACGATGGTCGCCCCAAGCGGACGGGTAATTGATCTCCTCATCTCCTTCACCTTCTAGTCGTTACAATACATAATTCGTTTCATCTATTTGTTTCTTCTTGAATGTTTAGGTACGTTTTGGACGGCAAGTGCTCATATAATCACAGCCGTAATTGGTTCGGGTGTGTTATCGTTAGCATGGGCAATCGCTCAGCTCGGATGGATTGCCGGTCCCATTGTGATGCTCTTGTTTGCCTTCATCGGTTACTATACCTCTTGTTTACTCGTTGATTGCTATCGCTCCAACGACTCGGTTAACGGCAAGAGAAACTACACTTACATGCATGCAGTCCGCTCCTTCCTAGGTATTGCAAAGTAACACAAACAAACCAAAAAAATTGATCAATCCATCTCTTGTTCCTTAAATAATATCGAGATTTGTTCCAGGCAGAGGTCAAACAGTAGCATGTGGAGTAATACAATACATGGATTTGATTGGAGTCGCAATCGGGTATACGATCGCATCGTCGATCAGCATGATGTGAGCACAAAACACAAACTTTCCCGAGTATAGCTTATAACTTTCGGTTTTCGATTTTCTGATTTTAACTCAAAAGCTTATTGTTCGTATCGTTCGTGTTTGGCAGGGCGGTTAAACGATCAAATTGCTTCCACAAAAGTGGGGGAAAGAATCCATGTCGTATGTCAAGCAATCCATTTATGGTATCATTTGGAGCTGTGGAGATAATTTTGTCTCAAATTCCAGATTTTGACCAAATTTGGTGGCTCTCCACAGTGGCTGCTATCATGTCGTTTACATATTCAACCATTGGCCTTGCTCTTGGGATTGCCAAAGTTGCAGGTTTTAACCTAAAAATCCCTTTATTTTCTTTCGATTCGTTTCAAAATGATTAAAGAAATGTTCGGGAGTTTGTCATGAAGAATCGTTCATAGCTCGTAGCTCATCGATGTTTAGATTTACACCCGAAAAAAGGGTTTGGGAGAATAAAGGTATTTGTTTAAAAAAATATTTTTTTTCCTAATGGGTTTAGGATTAACATTAAAAATAAATGTGTTTTAATTTTTTTTTTATAAAAAAATATCAATTTTTCCACTAATATTAAATAATTGTAATTATAAAATGGATATGTTAGGAGAAGTTCTCCGTTTATAATTAATAAAATTATAAATATATTTATTTTAAAAATTTTAAAATAAGCTTTTAAACCAAAAAAATAATAATAATAATTTAGAATGTATTTAATATAATTTTTTATATCTTAATGTAAAAAAAAAAAACTTTAAAAAGTGGGTTTATTTGATAGCTGTCTAGAGATGAATTATCCAAAAATAAATAAATAAATAAATAATTAATTAATTAATGTTATTTAAAGCATTTAAAAAAAATTAAATTTGATGTGCAGAAAATGGGAGTTTTAAAGGAACAGTGAGTGGCATAAGTGTGGAAACAGTGACTCAAACCCAAAAGATTTGGAGAACTTTTCAAGCACTTGGAGATATTGCTTTTGCCTATTCTTTTTCAGTTATCCTTATTGAAATTCAGGTATTTTTTATTTTTATTTCCTTTTTTATTTCCTTTTTTTAAATTAATCATAAATAATATTTTAAATGAAAAATCAAAATTATAAAATAAAGTGGAATAAATACAAAAGTTGGTGAGCAAAAATAAGCACATAATATTCCCGAATAATGTAAGGATTAATTTATTACTTTTTTTTTTTTCCCTTTATTATAAAAGCTTATTCTGTCCACATGGACACTTTTAATTGGATTAGATTAATTAAAGTTTTGTTTTGCTTTAATCAAATCACAAACATCCGGTTGACCGCTGGTGTGTTGCCTTTTGCTCGTATGTTATATAACATTGCTTTCTATCTCTTCCAATCTTGCTTTTGAACTATCTTTCAAATTCTTTCTGCTTCGTTTTCTAAAACTTTCTTTTAAAATCGGAGTTAGAGGCTTGAGTATACGTTGCGCGACTGTTGGCGACACTAATACAAAATTGGCTTAGGTCACTTGTTGTTGAGAACTGAGTGTCACACAATCGCAAAGTCTGCTCCCAAGAAAAGTACGATTGTGACAATGACTTATTTGAGTTGGGTTCGAAAACATTAAAACGTTGGGAGTTGCATTAGTTAAGGTTGACTAGAACTAACCCGACTTTATTGTTCATTTCCATGATCATTCCCTAAATCAGTTGATATGAGACTTTCATTCGACACCTCTCCTTAATCGAGGCTCGACTCCCTTTTCTTTTGGAGTCTTAGTCATTTTTTACTATGCCTTTGAGGAGCCTCAACTCCTCTTTTTTTTTTTTTTTTAAATGACTAAGTTTAAGGCATAACTCTGATACTATGTTTTAGATAAATACGACTCTCCACAATGGTATGATATTGTCCACTTTGAGCATAAGTTCTCATGGTTTAATAAAAGTCATCTGGACAATTGGGTAGGATTGAAAAATTATTCCAACCCGATTCAACCCGACTCAATCCAACTCATGAGGATTCTTAGTTCTTAGTTGACGGTTGCTAATTCTTTGTGTCGATATCGAACACAGAAGCACTTTTAACTACGACGGGGATTTGATTTAACATAATCGAGATTATATCGAACACAGGACACAATCAGATGCCCACCATCAGAAGCAAAGACAATGAAGAAGGCCTCAGGATTCAGCATTACAGTGACCACAATATTCTACCTACTTTGTGGATGCATGGGCTATGCAGCCTTTGGCAACAACGCCCCAGACAACCTCTTGACAGGCTTCGGCTTCTACAACCCGTATTGGCTCCTCGACATTGCCAACGTCGCCATCGTCATCCACCTCGTCGGCGCCTACCAAGTCTTCTGCCAGCCCGTCTTTGCCTTCGTCGAGAAGACAGCAGCTCAAACATGGCCCGACTCCGCCTTCATCACCAAACATTACAAGCTCCCTCTCTCTTCCTCTCGCTCGTACAATATCAACTTCTTTCGACTTGTATGGCGAACCCTTTTCGTGTGCTTCACCACCATCGTCGCCATGTTGCTCCCGTTCTTTAACGACATCGTCGGGATTATGGGGGCCCTCCAATTCTGGCCGTTGACGGTCTACTTTCCGGTTCAGATGTATATTGTTCAGAAGAAGATAGCTAAGTGGAGTGTGAAGTGGGTATGTGTTCAAACTATGAGCATGGGATGCCTTTTGATATCCATTGCTGCTGGTGTGGGCTCTCTTATTGGTGTTGTGCTTGATCTCAAGGTTTATAAGCCTTTCAACACAAGATATTGAACATTCAAAGCTTCGAACTTGTCATGTGTTGAGGAAAGGAAGTGGAAAGTCTTTCCTTTAACTCTTTTATGTATAATAGTTGTTTATGTATCTGCAATTTACGATCTCTAGTTCAAAATCAATACAATTTCGAGCAAGTTTAGAGGAAGAATTTGGCATAATTATAACGCTGTTATTAGAAACATTATTGAATGAAGACGTCACAATCTCCCCACTACCCACTTGAAATGTCACTTCACAAAAAAAAACTATCAGACACGTTGTAACGATGGTCGAAGATATGGTTGAAGATATTTAGTCTTCCACTACTGTTTACTGTATGCATCATGTTTGACCTTTGATTATAG

mRNA sequence

ACTCCCCCTAGCTCCATCACCTTTCTCCCTTTCCCCTCAACCTTTCCAACCCTACTCCGTCGTCATGGCCGTGCTCCCAATCAACGACTCCCCGAGCTTCGATGACGATGGTCGCCCCAAGCGGACGGGTACGTTTTGGACGGCAAGTGCTCATATAATCACAGCCGTAATTGGTTCGGGTGTGTTATCGTTAGCATGGGCAATCGCTCAGCTCGGATGGATTGCCGGTCCCATTGTGATGCTCTTGTTTGCCTTCATCGGTTACTATACCTCTTGTTTACTCGTTGATTGCTATCGCTCCAACGACTCGGTTAACGGCAAGAGAAACTACACTTACATGCATGCAGTCCGCTCCTTCCTAGGCAGAGGTCAAACAGTAGCATGTGGAGTAATACAATACATGGATTTGATTGGAGTCGCAATCGGGTATACGATCGCATCGTCGATCAGCATGATGGCGGTTAAACGATCAAATTGCTTCCACAAAAGTGGGGGAAAGAATCCATGTCGTATGTCAAGCAATCCATTTATGGTATCATTTGGAGCTGTGGAGATAATTTTGTCTCAAATTCCAGATTTTGACCAAATTTGGTGGCTCTCCACAGTGGCTGCTATCATGTCGTTTACATATTCAACCATTGGCCTTGCTCTTGGGATTGCCAAAGTTGCAGAAAATGGGAGTTTTAAAGGAACAGTGAGTGGCATAAGTGTGGAAACAGTGACTCAAACCCAAAAGATTTGGAGAACTTTTCAAGCACTTGGAGATATTGCTTTTGCCTATTCTTTTTCAGTTATCCTTATTGAAATTCAGGACACAATCAGATGCCCACCATCAGAAGCAAAGACAATGAAGAAGGCCTCAGGATTCAGCATTACAGTGACCACAATATTCTACCTACTTTGTGGATGCATGGGCTATGCAGCCTTTGGCAACAACGCCCCAGACAACCTCTTGACAGGCTTCGGCTTCTACAACCCGTATTGGCTCCTCGACATTGCCAACGTCGCCATCGTCATCCACCTCGTCGGCGCCTACCAAGTCTTCTGCCAGCCCGTCTTTGCCTTCGTCGAGAAGACAGCAGCTCAAACATGGCCCGACTCCGCCTTCATCACCAAACATTACAAGCTCCCTCTCTCTTCCTCTCGCTCGTACAATATCAACTTCTTTCGACTTGTATGGCGAACCCTTTTCGTGTGCTTCACCACCATCGTCGCCATGTTGCTCCCGTTCTTTAACGACATCGTCGGGATTATGGGGGCCCTCCAATTCTGGCCGTTGACGGTCTACTTTCCGGTTCAGATGTATATTGTTCAGAAGAAGATAGCTAAGTGGAGTGTGAAGTGGGTATGTGTTCAAACTATGAGCATGGGATGCCTTTTGATATCCATTGCTGCTGGTGTGGGCTCTCTTATTGGTGTTGTGCTTGATCTCAAGGTTTATAAGCCTTTCAACACAAGATATTGAACATTCAAAGCTTCGAACTTGTCATGTGTTGAGGAAAGGAAGTGGAAAGTCTTTCCTTTAACTCTTTTATGTATAATAGTTGTTTATGTATCTGCAATTTACGATCTCTAGTTCAAAATCAATACAATTTCGAGCAAGTTTAGAGGAAGAATTTGGCATAATTATAACGCTGTTATTAGAAACATTATTGAATGAAGACGTCACAATCTCCCCACTACCCACTTGAAATGTCACTTCACAAAAAAAAACTATCAGACACGTTGTAACGATGGTCGAAGATATGGTTGAAGATATTTAGTCTTCCACTACTGTTTACTGTATGCATCATGTTTGACCTTTGATTATAG

Coding sequence (CDS)

ATGGCCGTGCTCCCAATCAACGACTCCCCGAGCTTCGATGACGATGGTCGCCCCAAGCGGACGGGTACGTTTTGGACGGCAAGTGCTCATATAATCACAGCCGTAATTGGTTCGGGTGTGTTATCGTTAGCATGGGCAATCGCTCAGCTCGGATGGATTGCCGGTCCCATTGTGATGCTCTTGTTTGCCTTCATCGGTTACTATACCTCTTGTTTACTCGTTGATTGCTATCGCTCCAACGACTCGGTTAACGGCAAGAGAAACTACACTTACATGCATGCAGTCCGCTCCTTCCTAGGCAGAGGTCAAACAGTAGCATGTGGAGTAATACAATACATGGATTTGATTGGAGTCGCAATCGGGTATACGATCGCATCGTCGATCAGCATGATGGCGGTTAAACGATCAAATTGCTTCCACAAAAGTGGGGGAAAGAATCCATGTCGTATGTCAAGCAATCCATTTATGGTATCATTTGGAGCTGTGGAGATAATTTTGTCTCAAATTCCAGATTTTGACCAAATTTGGTGGCTCTCCACAGTGGCTGCTATCATGTCGTTTACATATTCAACCATTGGCCTTGCTCTTGGGATTGCCAAAGTTGCAGAAAATGGGAGTTTTAAAGGAACAGTGAGTGGCATAAGTGTGGAAACAGTGACTCAAACCCAAAAGATTTGGAGAACTTTTCAAGCACTTGGAGATATTGCTTTTGCCTATTCTTTTTCAGTTATCCTTATTGAAATTCAGGACACAATCAGATGCCCACCATCAGAAGCAAAGACAATGAAGAAGGCCTCAGGATTCAGCATTACAGTGACCACAATATTCTACCTACTTTGTGGATGCATGGGCTATGCAGCCTTTGGCAACAACGCCCCAGACAACCTCTTGACAGGCTTCGGCTTCTACAACCCGTATTGGCTCCTCGACATTGCCAACGTCGCCATCGTCATCCACCTCGTCGGCGCCTACCAAGTCTTCTGCCAGCCCGTCTTTGCCTTCGTCGAGAAGACAGCAGCTCAAACATGGCCCGACTCCGCCTTCATCACCAAACATTACAAGCTCCCTCTCTCTTCCTCTCGCTCGTACAATATCAACTTCTTTCGACTTGTATGGCGAACCCTTTTCGTGTGCTTCACCACCATCGTCGCCATGTTGCTCCCGTTCTTTAACGACATCGTCGGGATTATGGGGGCCCTCCAATTCTGGCCGTTGACGGTCTACTTTCCGGTTCAGATGTATATTGTTCAGAAGAAGATAGCTAAGTGGAGTGTGAAGTGGGTATGTGTTCAAACTATGAGCATGGGATGCCTTTTGATATCCATTGCTGCTGGTGTGGGCTCTCTTATTGGTGTTGTGCTTGATCTCAAGGTTTATAAGCCTTTCAACACAAGATATTGA

Protein sequence

MAVLPINDSPSFDDDGRPKRTGTFWTASAHIITAVIGSGVLSLAWAIAQLGWIAGPIVMLLFAFIGYYTSCLLVDCYRSNDSVNGKRNYTYMHAVRSFLGRGQTVACGVIQYMDLIGVAIGYTIASSISMMAVKRSNCFHKSGGKNPCRMSSNPFMVSFGAVEIILSQIPDFDQIWWLSTVAAIMSFTYSTIGLALGIAKVAENGSFKGTVSGISVETVTQTQKIWRTFQALGDIAFAYSFSVILIEIQDTIRCPPSEAKTMKKASGFSITVTTIFYLLCGCMGYAAFGNNAPDNLLTGFGFYNPYWLLDIANVAIVIHLVGAYQVFCQPVFAFVEKTAAQTWPDSAFITKHYKLPLSSSRSYNINFFRLVWRTLFVCFTTIVAMLLPFFNDIVGIMGALQFWPLTVYFPVQMYIVQKKIAKWSVKWVCVQTMSMGCLLISIAAGVGSLIGVVLDLKVYKPFNTRY
Homology
BLAST of CmaCh07G012680 vs. ExPASy Swiss-Prot
Match: Q38967 (Amino acid permease 2 OS=Arabidopsis thaliana OX=3702 GN=AAP2 PE=1 SV=1)

HSP 1 Score: 683.7 bits (1763), Expect = 1.4e-195
Identity = 326/456 (71.49%), Postives = 396/456 (86.84%), Query Frame = 0

Query: 12  FDDDGRPKRTGTFWTASAHIITAVIGSGVLSLAWAIAQLGWIAGPIVMLLFAFIGYYTSC 71
           FDDDGR KRTGT WTASAHIITAVIGSGVLSLAWAIAQLGWIAGP VMLLF+ +  Y+S 
Sbjct: 38  FDDDGRLKRTGTVWTASAHIITAVIGSGVLSLAWAIAQLGWIAGPAVMLLFSLVTLYSST 97

Query: 72  LLVDCYRSNDSVNGKRNYTYMHAVRSFLGRGQTVACGVIQYMDLIGVAIGYTIASSISMM 131
           LL DCYR+ D+V+GKRNYTYM AVRS LG  +   CG+IQY++L G+AIGYTIA+SISMM
Sbjct: 98  LLSDCYRTGDAVSGKRNYTYMDAVRSILGGFKFKICGLIQYLNLFGIAIGYTIAASISMM 157

Query: 132 AVKRSNCFHKSGGKNPCRMSSNPFMVSFGAVEIILSQIPDFDQIWWLSTVAAIMSFTYST 191
           A+KRSNCFHKSGGK+PC MSSNP+M+ FG  EI+LSQ+PDFDQIWW+S VAA+MSFTYS 
Sbjct: 158 AIKRSNCFHKSGGKDPCHMSSNPYMIVFGVAEILLSQVPDFDQIWWISIVAAVMSFTYSA 217

Query: 192 IGLALGIAKVAENGSFKGTVSGISVETVTQTQKIWRTFQALGDIAFAYSFSVILIEIQDT 251
           IGLALGI +VA NG FKG+++GIS+ TVTQTQKIWRTFQALGDIAFAYS+SV+LIEIQDT
Sbjct: 218 IGLALGIVQVAANGVFKGSLTGISIGTVTQTQKIWRTFQALGDIAFAYSYSVVLIEIQDT 277

Query: 252 IRCPPSEAKTMKKASGFSITVTTIFYLLCGCMGYAAFGNNAPDNLLTGFGFYNPYWLLDI 311
           +R PP+E+KTMKKA+  SI VTTIFY+LCG MGYAAFG+ AP NLLTGFGFYNP+WLLDI
Sbjct: 278 VRSPPAESKTMKKATKISIAVTTIFYMLCGSMGYAAFGDAAPGNLLTGFGFYNPFWLLDI 337

Query: 312 ANVAIVIHLVGAYQVFCQPVFAFVEKTAAQTWPDSAFITKHYKLPLSSSRS-YNINFFRL 371
           AN AIV+HLVGAYQVF QP+FAF+EK+ A+ +PD+ F++K +++ +   +S Y +N FR+
Sbjct: 338 ANAAIVVHLVGAYQVFAQPIFAFIEKSVAERYPDNDFLSKEFEIRIPGFKSPYKVNVFRM 397

Query: 372 VWRTLFVCFTTIVAMLLPFFNDIVGIMGALQFWPLTVYFPVQMYIVQKKIAKWSVKWVCV 431
           V+R+ FV  TT+++ML+PFFND+VGI+GAL FWPLTVYFPV+MYI Q+K+ KWS +WVC+
Sbjct: 398 VYRSGFVVTTTVISMLMPFFNDVVGILGALGFWPLTVYFPVEMYIKQRKVEKWSTRWVCL 457

Query: 432 QTMSMGCLLISIAAGVGSLIGVVLDLKVYKPFNTRY 467
           Q +S+ CL+IS+ AGVGS+ GV+LDLKVYKPF + Y
Sbjct: 458 QMLSVACLVISVVAGVGSIAGVMLDLKVYKPFKSTY 493

BLAST of CmaCh07G012680 vs. ExPASy Swiss-Prot
Match: Q9FN04 (Amino acid permease 4 OS=Arabidopsis thaliana OX=3702 GN=AAP4 PE=1 SV=1)

HSP 1 Score: 681.4 bits (1757), Expect = 7.2e-195
Identity = 328/456 (71.93%), Postives = 389/456 (85.31%), Query Frame = 0

Query: 12  FDDDGRPKRTGTFWTASAHIITAVIGSGVLSLAWAIAQLGWIAGPIVMLLFAFIGYYTSC 71
           FDDDGR KR+GT WTASAHIITAVIGSGVLSLAWAI QLGWIAGP VMLLF+F+ YY+S 
Sbjct: 11  FDDDGRLKRSGTVWTASAHIITAVIGSGVLSLAWAIGQLGWIAGPTVMLLFSFVTYYSST 70

Query: 72  LLVDCYRSNDSVNGKRNYTYMHAVRSFLGRGQTVACGVIQYMDLIGVAIGYTIASSISMM 131
           LL DCYR+ D V+GKRNYTYM AVRS LG  +   CG+IQY++L G+ +GYTIA+SISMM
Sbjct: 71  LLSDCYRTGDPVSGKRNYTYMDAVRSILGGFRFKICGLIQYLNLFGITVGYTIAASISMM 130

Query: 132 AVKRSNCFHKSGGKNPCRMSSNPFMVSFGAVEIILSQIPDFDQIWWLSTVAAIMSFTYST 191
           A+KRSNCFH+SGGKNPC MSSNP+M+ FG  EI+LSQI DFDQIWWLS VAAIMSFTYS 
Sbjct: 131 AIKRSNCFHESGGKNPCHMSSNPYMIMFGVTEILLSQIKDFDQIWWLSIVAAIMSFTYSA 190

Query: 192 IGLALGIAKVAENGSFKGTVSGISVETVTQTQKIWRTFQALGDIAFAYSFSVILIEIQDT 251
           IGLALGI +VA NG  KG+++GIS+  VTQTQKIWRTFQALGDIAFAYS+SV+LIEIQDT
Sbjct: 191 IGLALGIIQVAANGVVKGSLTGISIGAVTQTQKIWRTFQALGDIAFAYSYSVVLIEIQDT 250

Query: 252 IRCPPSEAKTMKKASGFSITVTTIFYLLCGCMGYAAFGNNAPDNLLTGFGFYNPYWLLDI 311
           +R PP+E+KTMK A+  SI VTT FY+LCGCMGYAAFG+ AP NLLTGFGFYNP+WLLD+
Sbjct: 251 VRSPPAESKTMKIATRISIAVTTTFYMLCGCMGYAAFGDKAPGNLLTGFGFYNPFWLLDV 310

Query: 312 ANVAIVIHLVGAYQVFCQPVFAFVEKTAAQTWPDSAFITKHYKLPLSSSRS-YNINFFRL 371
           AN AIVIHLVGAYQVF QP+FAF+EK AA  +PDS  +TK Y++ +   RS Y +N FR 
Sbjct: 311 ANAAIVIHLVGAYQVFAQPIFAFIEKQAAARFPDSDLVTKEYEIRIPGFRSPYKVNVFRA 370

Query: 372 VWRTLFVCFTTIVAMLLPFFNDIVGIMGALQFWPLTVYFPVQMYIVQKKIAKWSVKWVCV 431
           V+R+ FV  TT+++ML+PFFND+VGI+GAL FWPLTVYFPV+MYI Q+K+ +WS+KWVC+
Sbjct: 371 VYRSGFVVLTTVISMLMPFFNDVVGILGALGFWPLTVYFPVEMYIRQRKVERWSMKWVCL 430

Query: 432 QTMSMGCLLISIAAGVGSLIGVVLDLKVYKPFNTRY 467
           Q +S GCL+I++ AGVGS+ GV+LDLKVYKPF T Y
Sbjct: 431 QMLSCGCLMITLVAGVGSIAGVMLDLKVYKPFKTTY 466

BLAST of CmaCh07G012680 vs. ExPASy Swiss-Prot
Match: Q39134 (Amino acid permease 3 OS=Arabidopsis thaliana OX=3702 GN=AAP3 PE=1 SV=2)

HSP 1 Score: 680.6 bits (1755), Expect = 1.2e-194
Identity = 318/458 (69.43%), Postives = 391/458 (85.37%), Query Frame = 0

Query: 9   SPSFDDDGRPKRTGTFWTASAHIITAVIGSGVLSLAWAIAQLGWIAGPIVMLLFAFIGYY 68
           S   DDDG+ KRTG+ WTASAHIITAVIGSGVLSLAWA AQLGW+AGP+VMLLF+ + Y+
Sbjct: 19  SKYLDDDGKNKRTGSVWTASAHIITAVIGSGVLSLAWATAQLGWLAGPVVMLLFSAVTYF 78

Query: 69  TSCLLVDCYRSNDSVNGKRNYTYMHAVRSFLGRGQTVACGVIQYMDLIGVAIGYTIASSI 128
           TS LL  CYRS D ++GKRNYTYM AVRS LG  +   CG++QY+++ GVAIGYTIAS+I
Sbjct: 79  TSSLLAACYRSGDPISGKRNYTYMDAVRSNLGGVKVTLCGIVQYLNIFGVAIGYTIASAI 138

Query: 129 SMMAVKRSNCFHKSGGKNPCRMSSNPFMVSFGAVEIILSQIPDFDQIWWLSTVAAIMSFT 188
           SMMA+KRSNCFHKSGGK+PC M+SNP+M++FG V+I+ SQIPDFDQ+WWLS +AA+MSFT
Sbjct: 139 SMMAIKRSNCFHKSGGKDPCHMNSNPYMIAFGLVQILFSQIPDFDQLWWLSILAAVMSFT 198

Query: 189 YSTIGLALGIAKVAENGSFKGTVSGISVETVTQTQKIWRTFQALGDIAFAYSFSVILIEI 248
           YS+ GLALGIA+V  NG  KG+++GIS+  VT+TQKIWRTFQALGDIAFAYS+S+ILIEI
Sbjct: 199 YSSAGLALGIAQVVVNGKVKGSLTGISIGAVTETQKIWRTFQALGDIAFAYSYSIILIEI 258

Query: 249 QDTIRCPPSEAKTMKKASGFSITVTTIFYLLCGCMGYAAFGNNAPDNLLTGFGFYNPYWL 308
           QDT++ PPSE KTMKKA+  S++VTT+FY+LCGCMGYAAFG+ +P NLLTGFGFYNPYWL
Sbjct: 259 QDTVKSPPSEEKTMKKATLVSVSVTTMFYMLCGCMGYAAFGDLSPGNLLTGFGFYNPYWL 318

Query: 309 LDIANVAIVIHLVGAYQVFCQPVFAFVEKTAAQTWPDSAFITKHYKLPLSSSRSYNINFF 368
           LDIAN AIVIHL+GAYQV+CQP+FAF+EK A+  +PDS FI K  K+P+   +   +N F
Sbjct: 319 LDIANAAIVIHLIGAYQVYCQPLFAFIEKQASIQFPDSEFIAKDIKIPIPGFKPLRLNVF 378

Query: 369 RLVWRTLFVCFTTIVAMLLPFFNDIVGIMGALQFWPLTVYFPVQMYIVQKKIAKWSVKWV 428
           RL+WRT+FV  TT+++MLLPFFND+VG++GAL FWPLTVYFPV+MYI QKKI +WS +WV
Sbjct: 379 RLIWRTVFVIITTVISMLLPFFNDVVGLLGALGFWPLTVYFPVEMYIAQKKIPRWSTRWV 438

Query: 429 CVQTMSMGCLLISIAAGVGSLIGVVLDLKVYKPFNTRY 467
           C+Q  S+GCL++SIAA  GS+ GV+LDLK YKPF + Y
Sbjct: 439 CLQVFSLGCLVVSIAAAAGSIAGVLLDLKSYKPFRSEY 476

BLAST of CmaCh07G012680 vs. ExPASy Swiss-Prot
Match: Q8GUM3 (Amino acid permease 5 OS=Arabidopsis thaliana OX=3702 GN=AAP5 PE=1 SV=1)

HSP 1 Score: 641.3 bits (1653), Expect = 8.2e-183
Identity = 300/470 (63.83%), Postives = 383/470 (81.49%), Query Frame = 0

Query: 3   VLPINDSPSFDDDGRPKRTGTFWTASAHIITAVIGSGVLSLAWAIAQLGWIAGPIVMLLF 62
           VLP + S SFDDDGRPKRTGT WTASAHIITAVIGSGVLSLAWA+AQ+GWI GP+ MLLF
Sbjct: 11  VLPKHSSDSFDDDGRPKRTGTVWTASAHIITAVIGSGVLSLAWAVAQIGWIGGPVAMLLF 70

Query: 63  AFIGYYTSCLLVDCYRSNDSVNGKRNYTYMHAVRSFLGRGQTVACGVIQYMDLIGVAIGY 122
           +F+ +YTS LL  CYRS DSV GKRNYTYM A+ S LG  +   CGV+QY++L G AIGY
Sbjct: 71  SFVTFYTSTLLCSCYRSGDSVTGKRNYTYMDAIHSNLGGIKVKVCGVVQYVNLFGTAIGY 130

Query: 123 TIASSISMMAVKRSNCFHKSGGKNPCRMSSNPFMVSFGAVEIILSQIPDFDQIWWLSTVA 182
           TIAS+IS++A++R++C   +G  +PC ++ N +M++FG V+II SQIPDFDQ+WWLS VA
Sbjct: 131 TIASAISLVAIQRTSCQQMNGPNDPCHVNGNVYMIAFGIVQIIFSQIPDFDQLWWLSIVA 190

Query: 183 AIMSFTYSTIGLALGIAKVAENGSFKGTVSGISV------ETVTQTQKIWRTFQALGDIA 242
           A+MSF YS IGL LG++KV EN   KG+++G++V       TVT +QKIWRTFQ+LG+IA
Sbjct: 191 AVMSFAYSAIGLGLGVSKVVENKEIKGSLTGVTVGTVTLSGTVTSSQKIWRTFQSLGNIA 250

Query: 243 FAYSFSVILIEIQDTIRCPPSEAKTMKKASGFSITVTTIFYLLCGCMGYAAFGNNAPDNL 302
           FAYS+S+ILIEIQDT++ PP+E  TM+KA+  S+ VTT+FY+LCGC+GYAAFG+NAP NL
Sbjct: 251 FAYSYSMILIEIQDTVKSPPAEVNTMRKATFVSVAVTTVFYMLCGCVGYAAFGDNAPGNL 310

Query: 303 LTGFGFYNPYWLLDIANVAIVIHLVGAYQVFCQPVFAFVEKTAAQTWPDSAFITKHYKLP 362
           L   GF NPYWLLDIAN+AIVIHLVGAYQV+CQP+FAFVEK A++ +P+S F+TK  K+ 
Sbjct: 311 LAHGGFRNPYWLLDIANLAIVIHLVGAYQVYCQPLFAFVEKEASRRFPESEFVTKEIKIQ 370

Query: 363 LSSSRSYNINFFRLVWRTLFVCFTTIVAMLLPFFNDIVGIMGALQFWPLTVYFPVQMYIV 422
           L   + +N+N FRLVWRT FV  TT+++ML+PFFND+VG++GA+ FWPLTVYFPV+MYI 
Sbjct: 371 LFPGKPFNLNLFRLVWRTFFVMTTTLISMLMPFFNDVVGLLGAIGFWPLTVYFPVEMYIA 430

Query: 423 QKKIAKWSVKWVCVQTMSMGCLLISIAAGVGSLIGVVLDLKVYKPFNTRY 467
           QK + +W  KWVC+Q +S+ CL +S+AA  GS+IG+V DLKVYKPF + +
Sbjct: 431 QKNVPRWGTKWVCLQVLSVTCLFVSVAAAAGSVIGIVSDLKVYKPFQSEF 480

BLAST of CmaCh07G012680 vs. ExPASy Swiss-Prot
Match: P92934 (Amino acid permease 6 OS=Arabidopsis thaliana OX=3702 GN=AAP6 PE=1 SV=1)

HSP 1 Score: 561.6 bits (1446), Expect = 8.3e-159
Identity = 270/455 (59.34%), Postives = 349/455 (76.70%), Query Frame = 0

Query: 11  SFDDDGRPKRTGTFWTASAHIITAVIGSGVLSLAWAIAQLGWIAGPIVMLLFAFIGYYTS 70
           +FD+DGR KRTGT+ T SAHIITAVIGSGVLSLAWAIAQLGW+AGP V++ F+FI Y+TS
Sbjct: 24  NFDEDGRDKRTGTWMTGSAHIITAVIGSGVLSLAWAIAQLGWVAGPAVLMAFSFITYFTS 83

Query: 71  CLLVDCYRSNDSVNGKRNYTYMHAVRSFLGRGQTVACGVIQYMDLIGVAIGYTIASSISM 130
            +L DCYRS D V GKRNYTYM  VRS+LG  +   CG+ QY +LIG+ IGYTI +SISM
Sbjct: 84  TMLADCYRSPDPVTGKRNYTYMEVVRSYLGGRKVQLCGLAQYGNLIGITIGYTITASISM 143

Query: 131 MAVKRSNCFHKSGGKNPCRMSSNPFMVSFGAVEIILSQIPDFDQIWWLSTVAAIMSFTYS 190
           +AVKRSNCFHK+G    C  S+ PFM+ F  ++IILSQIP+F  + WLS +AA+MSF Y+
Sbjct: 144 VAVKRSNCFHKNGHNVKCATSNTPFMIIFAIIQIILSQIPNFHNLSWLSILAAVMSFCYA 203

Query: 191 TIGLALGIAKVAENGS-FKGTVSGISVE-TVTQTQKIWRTFQALGDIAFAYSFSVILIEI 250
           +IG+ L IAK A  G   + T++G++V   V+  +KIWRTFQA+GDIAFAY++S +LIEI
Sbjct: 204 SIGVGLSIAKAAGGGEHVRTTLTGVTVGIDVSGAEKIWRTFQAIGDIAFAYAYSTVLIEI 263

Query: 251 QDTIRC-PPSEAKTMKKASGFSITVTTIFYLLCGCMGYAAFGNNAPDNLLTGFGFYNPYW 310
           QDT++  PPSE K MK+AS   ++ TT FY+LCGC+GYAAFGN+AP N LTGFGFY P+W
Sbjct: 264 QDTLKAGPPSENKAMKRASLVGVSTTTFFYMLCGCVGYAAFGNDAPGNFLTGFGFYEPFW 323

Query: 311 LLDIANVAIVIHLVGAYQVFCQPVFAFVEKTAAQTWPDSAFITKHYKLPLSSSRSYNINF 370
           L+D ANV I +HL+GAYQVFCQP+F FVE  +A+ WPD+ FIT  YK+ +     ++INF
Sbjct: 324 LIDFANVCIAVHLIGAYQVFCQPIFQFVESQSAKRWPDNKFITGEYKIHVPCCGDFSINF 383

Query: 371 FRLVWRTLFVCFTTIVAMLLPFFNDIVGIMGALQFWPLTVYFPVQMYIVQKKIAKWSVKW 430
            RLVWRT +V  T +VAM+ PFFND +G++GA  FWPLTVYFP++M+I QKKI K+S  W
Sbjct: 384 LRLVWRTSYVVVTAVVAMIFPFFNDFLGLIGAASFWPLTVYFPIEMHIAQKKIPKFSFTW 443

Query: 431 VCVQTMSMGCLLISIAAGVGSLIGVVLDLKVYKPF 463
             ++ +S  C ++S+ A  GS+ G++  LK +KPF
Sbjct: 444 TWLKILSWTCFIVSLVAAAGSVQGLIQSLKDFKPF 478

BLAST of CmaCh07G012680 vs. ExPASy TrEMBL
Match: A0A6J1HRN4 (amino acid permease 4-like OS=Cucurbita maxima OX=3661 GN=LOC111466004 PE=3 SV=1)

HSP 1 Score: 933.3 bits (2411), Expect = 3.9e-268
Identity = 466/466 (100.00%), Postives = 466/466 (100.00%), Query Frame = 0

Query: 1   MAVLPINDSPSFDDDGRPKRTGTFWTASAHIITAVIGSGVLSLAWAIAQLGWIAGPIVML 60
           MAVLPINDSPSFDDDGRPKRTGTFWTASAHIITAVIGSGVLSLAWAIAQLGWIAGPIVML
Sbjct: 1   MAVLPINDSPSFDDDGRPKRTGTFWTASAHIITAVIGSGVLSLAWAIAQLGWIAGPIVML 60

Query: 61  LFAFIGYYTSCLLVDCYRSNDSVNGKRNYTYMHAVRSFLGRGQTVACGVIQYMDLIGVAI 120
           LFAFIGYYTSCLLVDCYRSNDSVNGKRNYTYMHAVRSFLGRGQTVACGVIQYMDLIGVAI
Sbjct: 61  LFAFIGYYTSCLLVDCYRSNDSVNGKRNYTYMHAVRSFLGRGQTVACGVIQYMDLIGVAI 120

Query: 121 GYTIASSISMMAVKRSNCFHKSGGKNPCRMSSNPFMVSFGAVEIILSQIPDFDQIWWLST 180
           GYTIASSISMMAVKRSNCFHKSGGKNPCRMSSNPFMVSFGAVEIILSQIPDFDQIWWLST
Sbjct: 121 GYTIASSISMMAVKRSNCFHKSGGKNPCRMSSNPFMVSFGAVEIILSQIPDFDQIWWLST 180

Query: 181 VAAIMSFTYSTIGLALGIAKVAENGSFKGTVSGISVETVTQTQKIWRTFQALGDIAFAYS 240
           VAAIMSFTYSTIGLALGIAKVAENGSFKGTVSGISVETVTQTQKIWRTFQALGDIAFAYS
Sbjct: 181 VAAIMSFTYSTIGLALGIAKVAENGSFKGTVSGISVETVTQTQKIWRTFQALGDIAFAYS 240

Query: 241 FSVILIEIQDTIRCPPSEAKTMKKASGFSITVTTIFYLLCGCMGYAAFGNNAPDNLLTGF 300
           FSVILIEIQDTIRCPPSEAKTMKKASGFSITVTTIFYLLCGCMGYAAFGNNAPDNLLTGF
Sbjct: 241 FSVILIEIQDTIRCPPSEAKTMKKASGFSITVTTIFYLLCGCMGYAAFGNNAPDNLLTGF 300

Query: 301 GFYNPYWLLDIANVAIVIHLVGAYQVFCQPVFAFVEKTAAQTWPDSAFITKHYKLPLSSS 360
           GFYNPYWLLDIANVAIVIHLVGAYQVFCQPVFAFVEKTAAQTWPDSAFITKHYKLPLSSS
Sbjct: 301 GFYNPYWLLDIANVAIVIHLVGAYQVFCQPVFAFVEKTAAQTWPDSAFITKHYKLPLSSS 360

Query: 361 RSYNINFFRLVWRTLFVCFTTIVAMLLPFFNDIVGIMGALQFWPLTVYFPVQMYIVQKKI 420
           RSYNINFFRLVWRTLFVCFTTIVAMLLPFFNDIVGIMGALQFWPLTVYFPVQMYIVQKKI
Sbjct: 361 RSYNINFFRLVWRTLFVCFTTIVAMLLPFFNDIVGIMGALQFWPLTVYFPVQMYIVQKKI 420

Query: 421 AKWSVKWVCVQTMSMGCLLISIAAGVGSLIGVVLDLKVYKPFNTRY 467
           AKWSVKWVCVQTMSMGCLLISIAAGVGSLIGVVLDLKVYKPFNTRY
Sbjct: 421 AKWSVKWVCVQTMSMGCLLISIAAGVGSLIGVVLDLKVYKPFNTRY 466

BLAST of CmaCh07G012680 vs. ExPASy TrEMBL
Match: A0A6J1EAD3 (amino acid permease 4-like OS=Cucurbita moschata OX=3662 GN=LOC111432325 PE=3 SV=1)

HSP 1 Score: 902.1 bits (2330), Expect = 9.5e-259
Identity = 447/466 (95.92%), Postives = 454/466 (97.42%), Query Frame = 0

Query: 1   MAVLPINDSPSFDDDGRPKRTGTFWTASAHIITAVIGSGVLSLAWAIAQLGWIAGPIVML 60
           MA+LPINDSPS DDDGRPKRTGTFWTASAHIITAVIGSGVLSLAWAIAQLGWIAGPIVML
Sbjct: 1   MAMLPINDSPSSDDDGRPKRTGTFWTASAHIITAVIGSGVLSLAWAIAQLGWIAGPIVML 60

Query: 61  LFAFIGYYTSCLLVDCYRSNDSVNGKRNYTYMHAVRSFLGRGQTVACGVIQYMDLIGVAI 120
           LFAFI YYTSCLL DCYRSNDSVN KRNYTYMHAVRSFLGRGQT+ CGVIQYMDLIGVAI
Sbjct: 61  LFAFISYYTSCLLTDCYRSNDSVNAKRNYTYMHAVRSFLGRGQTIVCGVIQYMDLIGVAI 120

Query: 121 GYTIASSISMMAVKRSNCFHKSGGKNPCRMSSNPFMVSFGAVEIILSQIPDFDQIWWLST 180
           GYTIASSISMMAVKRSNCFHKSGGKNPCRMSSNPFMVSFG VEIILSQIPDFDQIWWLST
Sbjct: 121 GYTIASSISMMAVKRSNCFHKSGGKNPCRMSSNPFMVSFGVVEIILSQIPDFDQIWWLST 180

Query: 181 VAAIMSFTYSTIGLALGIAKVAENGSFKGTVSGISVETVTQTQKIWRTFQALGDIAFAYS 240
           VAAIMSFTYSTIGLALGIAKVAENGSFKGTVSG+SV TVTQTQKIWRTFQALGDIAFAYS
Sbjct: 181 VAAIMSFTYSTIGLALGIAKVAENGSFKGTVSGMSVGTVTQTQKIWRTFQALGDIAFAYS 240

Query: 241 FSVILIEIQDTIRCPPSEAKTMKKASGFSITVTTIFYLLCGCMGYAAFGNNAPDNLLTGF 300
           FSVILIEIQDTIRCPPSEAKTMKKASGFSI VTTIFYLLCGCMGYAAFGNNAP NLLTGF
Sbjct: 241 FSVILIEIQDTIRCPPSEAKTMKKASGFSIAVTTIFYLLCGCMGYAAFGNNAPGNLLTGF 300

Query: 301 GFYNPYWLLDIANVAIVIHLVGAYQVFCQPVFAFVEKTAAQTWPDSAFITKHYKLPLSSS 360
           GFYNPYWLLDIANVAIV+HLVGAYQVFCQPVFAFVEK AAQTWPDSAFITKHYKLPLSSS
Sbjct: 301 GFYNPYWLLDIANVAIVVHLVGAYQVFCQPVFAFVEKMAAQTWPDSAFITKHYKLPLSSS 360

Query: 361 RSYNINFFRLVWRTLFVCFTTIVAMLLPFFNDIVGIMGALQFWPLTVYFPVQMYIVQKKI 420
           RSYNINFFRLVWRTLFVCFTT++AMLLPFFND+VGIMGA QFWPLTVYFPVQMYIVQKKI
Sbjct: 361 RSYNINFFRLVWRTLFVCFTTVIAMLLPFFNDVVGIMGAFQFWPLTVYFPVQMYIVQKKI 420

Query: 421 AKWSVKWVCVQTMSMGCLLISIAAGVGSLIGVVLDLKVYKPFNTRY 467
           AKWSVKWVCVQTMSMGCLLISIAAGVGSLIGVVLDLKVYKPF TRY
Sbjct: 421 AKWSVKWVCVQTMSMGCLLISIAAGVGSLIGVVLDLKVYKPFITRY 466

BLAST of CmaCh07G012680 vs. ExPASy TrEMBL
Match: A0A6J1I184 (amino acid permease 4-like OS=Cucurbita maxima OX=3661 GN=LOC111469952 PE=3 SV=1)

HSP 1 Score: 844.3 bits (2180), Expect = 2.4e-241
Identity = 410/466 (87.98%), Postives = 440/466 (94.42%), Query Frame = 0

Query: 1   MAVLPINDSPSFDDDGRPKRTGTFWTASAHIITAVIGSGVLSLAWAIAQLGWIAGPIVML 60
           MAVLPIND+PSFDDDGRPKRTGTFWTASAHIITAVIGSGVLSLAWAIAQLGWIAGP VML
Sbjct: 1   MAVLPINDAPSFDDDGRPKRTGTFWTASAHIITAVIGSGVLSLAWAIAQLGWIAGPSVML 60

Query: 61  LFAFIGYYTSCLLVDCYRSNDSVNGKRNYTYMHAVRSFLGRGQTVACGVIQYMDLIGVAI 120
           LFAFIGYYTSCLL DCYRS+D VNGKRNYTYMHAVRS LGRGQT ACGV+QY++LIG++I
Sbjct: 61  LFAFIGYYTSCLLADCYRSSDPVNGKRNYTYMHAVRSLLGRGQTTACGVLQYVNLIGISI 120

Query: 121 GYTIASSISMMAVKRSNCFHKSGGKNPCRMSSNPFMVSFGAVEIILSQIPDFDQIWWLST 180
           GYTIASSISMMAVKRSNCFH SGGKNPC MSSNPFMVSFG +EIILSQIPDFDQIWWLST
Sbjct: 121 GYTIASSISMMAVKRSNCFHSSGGKNPCHMSSNPFMVSFGVMEIILSQIPDFDQIWWLST 180

Query: 181 VAAIMSFTYSTIGLALGIAKVAENGSFKGTVSGISVETVTQTQKIWRTFQALGDIAFAYS 240
           VAA+MSFTYSTIGL LGIAKVAE GSFKGTVSGISV  + ++QKIWRTFQALGDIAFAYS
Sbjct: 181 VAAVMSFTYSTIGLGLGIAKVAETGSFKGTVSGISVGPINESQKIWRTFQALGDIAFAYS 240

Query: 241 FSVILIEIQDTIRCPPSEAKTMKKASGFSITVTTIFYLLCGCMGYAAFGNNAPDNLLTGF 300
           FS+ILIEIQDTIRCPPSEAKTMKKA+GFSI +TTIFY+LCGCMGYAAFGN+AP NLLTGF
Sbjct: 241 FSIILIEIQDTIRCPPSEAKTMKKATGFSIALTTIFYMLCGCMGYAAFGNDAPGNLLTGF 300

Query: 301 GFYNPYWLLDIANVAIVIHLVGAYQVFCQPVFAFVEKTAAQTWPDSAFITKHYKLPLSSS 360
           GFYNP+WLLD+ANVAIV+HLVGAYQVF QPVFAFVEK AAQ WPDS FITKH+KL +SSS
Sbjct: 301 GFYNPFWLLDVANVAIVVHLVGAYQVFSQPVFAFVEKKAAQAWPDSPFITKHHKLSISSS 360

Query: 361 RSYNINFFRLVWRTLFVCFTTIVAMLLPFFNDIVGIMGALQFWPLTVYFPVQMYIVQKKI 420
           RSYN+N FRLVWR+LFVCFTT+VAMLLPFFND+VGI+GALQFWPLTVYFPVQMYIVQKKI
Sbjct: 361 RSYNVNLFRLVWRSLFVCFTTVVAMLLPFFNDVVGIIGALQFWPLTVYFPVQMYIVQKKI 420

Query: 421 AKWSVKWVCVQTMSMGCLLISIAAGVGSLIGVVLDLKVYKPFNTRY 467
            KWS+KWVCVQTMSMGCLLIS AA VGS+IGV+LDLKVYKPF T Y
Sbjct: 421 PKWSLKWVCVQTMSMGCLLISFAAVVGSVIGVMLDLKVYKPFKTMY 466

BLAST of CmaCh07G012680 vs. ExPASy TrEMBL
Match: A0A6J1EXV8 (amino acid permease 4-like OS=Cucurbita moschata OX=3662 GN=LOC111439300 PE=3 SV=1)

HSP 1 Score: 838.2 bits (2164), Expect = 1.7e-239
Identity = 404/466 (86.70%), Postives = 439/466 (94.21%), Query Frame = 0

Query: 1   MAVLPINDSPSFDDDGRPKRTGTFWTASAHIITAVIGSGVLSLAWAIAQLGWIAGPIVML 60
           MAVLPIND+ SFDDDGRPKRTGTFWTASAHIITAVIGSGVLSLAWAIAQLGW+AGP VML
Sbjct: 1   MAVLPINDAASFDDDGRPKRTGTFWTASAHIITAVIGSGVLSLAWAIAQLGWVAGPSVML 60

Query: 61  LFAFIGYYTSCLLVDCYRSNDSVNGKRNYTYMHAVRSFLGRGQTVACGVIQYMDLIGVAI 120
           LFAFIGYYTSCLL DCYRS+D VNGKRNYTYMHAVRS LGR QT ACGV+QY++L+G++I
Sbjct: 61  LFAFIGYYTSCLLADCYRSSDPVNGKRNYTYMHAVRSLLGRSQTTACGVLQYVNLVGISI 120

Query: 121 GYTIASSISMMAVKRSNCFHKSGGKNPCRMSSNPFMVSFGAVEIILSQIPDFDQIWWLST 180
           GYTIAS+ISMMAVKRSNCFH SGGKNPC MSSNPFMVSFG +EIILSQIPDFDQIWWLS+
Sbjct: 121 GYTIASAISMMAVKRSNCFHSSGGKNPCHMSSNPFMVSFGVMEIILSQIPDFDQIWWLSS 180

Query: 181 VAAIMSFTYSTIGLALGIAKVAENGSFKGTVSGISVETVTQTQKIWRTFQALGDIAFAYS 240
           VAA+MSFTYSTIGL LGIAKVAE GSFKGTVSGISV T+ Q+QKIWRTFQALGDIAFAYS
Sbjct: 181 VAAVMSFTYSTIGLGLGIAKVAETGSFKGTVSGISVGTINQSQKIWRTFQALGDIAFAYS 240

Query: 241 FSVILIEIQDTIRCPPSEAKTMKKASGFSITVTTIFYLLCGCMGYAAFGNNAPDNLLTGF 300
           FS+ILIEIQDT+RCPPSEAKTMKKA+GFSI +TTIFY+LCGCMGYAAFGN+AP NLLTGF
Sbjct: 241 FSIILIEIQDTVRCPPSEAKTMKKATGFSIALTTIFYMLCGCMGYAAFGNDAPGNLLTGF 300

Query: 301 GFYNPYWLLDIANVAIVIHLVGAYQVFCQPVFAFVEKTAAQTWPDSAFITKHYKLPLSSS 360
           GFYNP+WLLDIAN+AIV+HLVGAYQVF QPVFAFVEK AAQ WPDS FITKH+KL +SSS
Sbjct: 301 GFYNPFWLLDIANIAIVVHLVGAYQVFSQPVFAFVEKKAAQAWPDSPFITKHHKLSISSS 360

Query: 361 RSYNINFFRLVWRTLFVCFTTIVAMLLPFFNDIVGIMGALQFWPLTVYFPVQMYIVQKKI 420
           RSYN+N FRL+WR+LFVCFTT+VAMLLPFFND+VGI+GALQFWPLTVYFPVQMYIVQKKI
Sbjct: 361 RSYNVNLFRLIWRSLFVCFTTVVAMLLPFFNDVVGIIGALQFWPLTVYFPVQMYIVQKKI 420

Query: 421 AKWSVKWVCVQTMSMGCLLISIAAGVGSLIGVVLDLKVYKPFNTRY 467
            KWS+KWVCVQTMSMGCLLIS AA VGS+IGV+LDLKVYKPF T Y
Sbjct: 421 PKWSLKWVCVQTMSMGCLLISFAAVVGSVIGVMLDLKVYKPFKTTY 466

BLAST of CmaCh07G012680 vs. ExPASy TrEMBL
Match: A0A6J1DSY4 (amino acid permease 4-like OS=Momordica charantia OX=3673 GN=LOC111023641 PE=3 SV=1)

HSP 1 Score: 825.9 bits (2132), Expect = 8.7e-236
Identity = 404/466 (86.70%), Postives = 431/466 (92.49%), Query Frame = 0

Query: 1   MAVLPINDSPSFDDDGRPKRTGTFWTASAHIITAVIGSGVLSLAWAIAQLGWIAGPIVML 60
           MA+LPINDS   DDDGR KRTGTFWTASAHIITAVIGSGVLSLAWAIAQLGW+AGP VML
Sbjct: 1   MAMLPINDSAILDDDGRLKRTGTFWTASAHIITAVIGSGVLSLAWAIAQLGWVAGPAVML 60

Query: 61  LFAFIGYYTSCLLVDCYRSNDSVNGKRNYTYMHAVRSFLGRGQTVACGVIQYMDLIGVAI 120
           LFAFIGYYTSCLL DCYRS D +NGKRNYTYMHAVRS LG  QT ACG++QYM+LIG+AI
Sbjct: 61  LFAFIGYYTSCLLADCYRSGDPINGKRNYTYMHAVRSLLGGAQTTACGIMQYMNLIGIAI 120

Query: 121 GYTIASSISMMAVKRSNCFHKSGGKNPCRMSSNPFMVSFGAVEIILSQIPDFDQIWWLST 180
           GYTIASSISMMA+KRSNCFH SGGKNPC MSSNPFM+SFG +EI LSQIPDFDQIWWLST
Sbjct: 121 GYTIASSISMMAIKRSNCFHSSGGKNPCHMSSNPFMISFGVMEIFLSQIPDFDQIWWLST 180

Query: 181 VAAIMSFTYSTIGLALGIAKVAENGSFKGTVSGISVETVTQTQKIWRTFQALGDIAFAYS 240
           VAAIMSFTYSTIGL LGIAKVAE+GSFKGT+SGI V TVTQ+QKIWRTFQALGDIAFAYS
Sbjct: 181 VAAIMSFTYSTIGLGLGIAKVAESGSFKGTLSGIGVGTVTQSQKIWRTFQALGDIAFAYS 240

Query: 241 FSVILIEIQDTIRCPPSEAKTMKKASGFSITVTTIFYLLCGCMGYAAFGNNAPDNLLTGF 300
           FS+ILIEIQDTIRCPPSEAKTMKKA+G SI VTT FYLLCGCMGYAAFGN+AP NLLTGF
Sbjct: 241 FSIILIEIQDTIRCPPSEAKTMKKATGLSIAVTTTFYLLCGCMGYAAFGNSAPGNLLTGF 300

Query: 301 GFYNPYWLLDIANVAIVIHLVGAYQVFCQPVFAFVEKTAAQTWPDSAFITKHYKLPLSSS 360
           GFYNP+WLLDIANVAIV+HLVGAYQVFCQPVFAFVEK AAQ WPDS FITK +KL L   
Sbjct: 301 GFYNPFWLLDIANVAIVVHLVGAYQVFCQPVFAFVEKKAAQAWPDSTFITKEHKLSL-FR 360

Query: 361 RSYNINFFRLVWRTLFVCFTTIVAMLLPFFNDIVGIMGALQFWPLTVYFPVQMYIVQKKI 420
           RSYN+N FRLVWR+LFVCFTT+VAMLLPFFND+VGI+GALQFWPLTVYFPVQMYIVQKKI
Sbjct: 361 RSYNVNMFRLVWRSLFVCFTTVVAMLLPFFNDVVGIIGALQFWPLTVYFPVQMYIVQKKI 420

Query: 421 AKWSVKWVCVQTMSMGCLLISIAAGVGSLIGVVLDLKVYKPFNTRY 467
            KWSVKWVCVQTMSMGCLLIS+AA VGS+IGV+LDLKVYKPF TRY
Sbjct: 421 PKWSVKWVCVQTMSMGCLLISVAAAVGSVIGVMLDLKVYKPFKTRY 465

BLAST of CmaCh07G012680 vs. NCBI nr
Match: XP_022966303.1 (amino acid permease 4-like [Cucurbita maxima])

HSP 1 Score: 933.3 bits (2411), Expect = 8.0e-268
Identity = 466/466 (100.00%), Postives = 466/466 (100.00%), Query Frame = 0

Query: 1   MAVLPINDSPSFDDDGRPKRTGTFWTASAHIITAVIGSGVLSLAWAIAQLGWIAGPIVML 60
           MAVLPINDSPSFDDDGRPKRTGTFWTASAHIITAVIGSGVLSLAWAIAQLGWIAGPIVML
Sbjct: 1   MAVLPINDSPSFDDDGRPKRTGTFWTASAHIITAVIGSGVLSLAWAIAQLGWIAGPIVML 60

Query: 61  LFAFIGYYTSCLLVDCYRSNDSVNGKRNYTYMHAVRSFLGRGQTVACGVIQYMDLIGVAI 120
           LFAFIGYYTSCLLVDCYRSNDSVNGKRNYTYMHAVRSFLGRGQTVACGVIQYMDLIGVAI
Sbjct: 61  LFAFIGYYTSCLLVDCYRSNDSVNGKRNYTYMHAVRSFLGRGQTVACGVIQYMDLIGVAI 120

Query: 121 GYTIASSISMMAVKRSNCFHKSGGKNPCRMSSNPFMVSFGAVEIILSQIPDFDQIWWLST 180
           GYTIASSISMMAVKRSNCFHKSGGKNPCRMSSNPFMVSFGAVEIILSQIPDFDQIWWLST
Sbjct: 121 GYTIASSISMMAVKRSNCFHKSGGKNPCRMSSNPFMVSFGAVEIILSQIPDFDQIWWLST 180

Query: 181 VAAIMSFTYSTIGLALGIAKVAENGSFKGTVSGISVETVTQTQKIWRTFQALGDIAFAYS 240
           VAAIMSFTYSTIGLALGIAKVAENGSFKGTVSGISVETVTQTQKIWRTFQALGDIAFAYS
Sbjct: 181 VAAIMSFTYSTIGLALGIAKVAENGSFKGTVSGISVETVTQTQKIWRTFQALGDIAFAYS 240

Query: 241 FSVILIEIQDTIRCPPSEAKTMKKASGFSITVTTIFYLLCGCMGYAAFGNNAPDNLLTGF 300
           FSVILIEIQDTIRCPPSEAKTMKKASGFSITVTTIFYLLCGCMGYAAFGNNAPDNLLTGF
Sbjct: 241 FSVILIEIQDTIRCPPSEAKTMKKASGFSITVTTIFYLLCGCMGYAAFGNNAPDNLLTGF 300

Query: 301 GFYNPYWLLDIANVAIVIHLVGAYQVFCQPVFAFVEKTAAQTWPDSAFITKHYKLPLSSS 360
           GFYNPYWLLDIANVAIVIHLVGAYQVFCQPVFAFVEKTAAQTWPDSAFITKHYKLPLSSS
Sbjct: 301 GFYNPYWLLDIANVAIVIHLVGAYQVFCQPVFAFVEKTAAQTWPDSAFITKHYKLPLSSS 360

Query: 361 RSYNINFFRLVWRTLFVCFTTIVAMLLPFFNDIVGIMGALQFWPLTVYFPVQMYIVQKKI 420
           RSYNINFFRLVWRTLFVCFTTIVAMLLPFFNDIVGIMGALQFWPLTVYFPVQMYIVQKKI
Sbjct: 361 RSYNINFFRLVWRTLFVCFTTIVAMLLPFFNDIVGIMGALQFWPLTVYFPVQMYIVQKKI 420

Query: 421 AKWSVKWVCVQTMSMGCLLISIAAGVGSLIGVVLDLKVYKPFNTRY 467
           AKWSVKWVCVQTMSMGCLLISIAAGVGSLIGVVLDLKVYKPFNTRY
Sbjct: 421 AKWSVKWVCVQTMSMGCLLISIAAGVGSLIGVVLDLKVYKPFNTRY 466

BLAST of CmaCh07G012680 vs. NCBI nr
Match: KAG6595602.1 (Amino acid permease 2, partial [Cucurbita argyrosperma subsp. sororia] >KAG7027574.1 Amino acid permease 2, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 902.9 bits (2332), Expect = 1.2e-258
Identity = 449/466 (96.35%), Postives = 454/466 (97.42%), Query Frame = 0

Query: 1   MAVLPINDSPSFDDDGRPKRTGTFWTASAHIITAVIGSGVLSLAWAIAQLGWIAGPIVML 60
           MAVLPINDSPS DDDGRPKRTGTFWTASAHIITAVIGSGVLSLAWAIAQLGWIAGPIVML
Sbjct: 1   MAVLPINDSPSSDDDGRPKRTGTFWTASAHIITAVIGSGVLSLAWAIAQLGWIAGPIVML 60

Query: 61  LFAFIGYYTSCLLVDCYRSNDSVNGKRNYTYMHAVRSFLGRGQTVACGVIQYMDLIGVAI 120
           LFAFI YYTSCLL DCYRSNDSVN KRNYTYMHAVRSFLGRGQTV CGVIQYMDLIGVAI
Sbjct: 61  LFAFISYYTSCLLTDCYRSNDSVNAKRNYTYMHAVRSFLGRGQTVVCGVIQYMDLIGVAI 120

Query: 121 GYTIASSISMMAVKRSNCFHKSGGKNPCRMSSNPFMVSFGAVEIILSQIPDFDQIWWLST 180
           GYTIASSISMMAVKRSNCFHKSGGKNPCRMSSNPFMVSFG VEIILSQIPDFDQIWWLST
Sbjct: 121 GYTIASSISMMAVKRSNCFHKSGGKNPCRMSSNPFMVSFGVVEIILSQIPDFDQIWWLST 180

Query: 181 VAAIMSFTYSTIGLALGIAKVAENGSFKGTVSGISVETVTQTQKIWRTFQALGDIAFAYS 240
           VAAIMSFTYSTIGLALGIAKVAENGSFKGTVSG+SV TVTQTQKIWRTFQALGDIAFAYS
Sbjct: 181 VAAIMSFTYSTIGLALGIAKVAENGSFKGTVSGMSVGTVTQTQKIWRTFQALGDIAFAYS 240

Query: 241 FSVILIEIQDTIRCPPSEAKTMKKASGFSITVTTIFYLLCGCMGYAAFGNNAPDNLLTGF 300
           FSVILIEIQDTIRCPPSEAKTMKKA+GFSI VTTIFYLLCGCMGYAAFGNNAP NLLTGF
Sbjct: 241 FSVILIEIQDTIRCPPSEAKTMKKATGFSIAVTTIFYLLCGCMGYAAFGNNAPGNLLTGF 300

Query: 301 GFYNPYWLLDIANVAIVIHLVGAYQVFCQPVFAFVEKTAAQTWPDSAFITKHYKLPLSSS 360
           GFYNPYWLLDIANVAIV+HLVGAYQVFCQPVFAFVEK AAQTWPDSAFITKHYKLPLSSS
Sbjct: 301 GFYNPYWLLDIANVAIVVHLVGAYQVFCQPVFAFVEKMAAQTWPDSAFITKHYKLPLSSS 360

Query: 361 RSYNINFFRLVWRTLFVCFTTIVAMLLPFFNDIVGIMGALQFWPLTVYFPVQMYIVQKKI 420
           RSYNINFFRLVWRTLFVCFTT+VAMLLPFFND+VGIMGA QFWPLTVYFPVQMYIVQKKI
Sbjct: 361 RSYNINFFRLVWRTLFVCFTTVVAMLLPFFNDVVGIMGAFQFWPLTVYFPVQMYIVQKKI 420

Query: 421 AKWSVKWVCVQTMSMGCLLISIAAGVGSLIGVVLDLKVYKPFNTRY 467
           AKWSVKWVCVQTMSMGCLLISIAAGVGSLIGVVLDLKVYKPF TRY
Sbjct: 421 AKWSVKWVCVQTMSMGCLLISIAAGVGSLIGVVLDLKVYKPFITRY 466

BLAST of CmaCh07G012680 vs. NCBI nr
Match: XP_022924922.1 (amino acid permease 4-like [Cucurbita moschata])

HSP 1 Score: 902.1 bits (2330), Expect = 2.0e-258
Identity = 447/466 (95.92%), Postives = 454/466 (97.42%), Query Frame = 0

Query: 1   MAVLPINDSPSFDDDGRPKRTGTFWTASAHIITAVIGSGVLSLAWAIAQLGWIAGPIVML 60
           MA+LPINDSPS DDDGRPKRTGTFWTASAHIITAVIGSGVLSLAWAIAQLGWIAGPIVML
Sbjct: 1   MAMLPINDSPSSDDDGRPKRTGTFWTASAHIITAVIGSGVLSLAWAIAQLGWIAGPIVML 60

Query: 61  LFAFIGYYTSCLLVDCYRSNDSVNGKRNYTYMHAVRSFLGRGQTVACGVIQYMDLIGVAI 120
           LFAFI YYTSCLL DCYRSNDSVN KRNYTYMHAVRSFLGRGQT+ CGVIQYMDLIGVAI
Sbjct: 61  LFAFISYYTSCLLTDCYRSNDSVNAKRNYTYMHAVRSFLGRGQTIVCGVIQYMDLIGVAI 120

Query: 121 GYTIASSISMMAVKRSNCFHKSGGKNPCRMSSNPFMVSFGAVEIILSQIPDFDQIWWLST 180
           GYTIASSISMMAVKRSNCFHKSGGKNPCRMSSNPFMVSFG VEIILSQIPDFDQIWWLST
Sbjct: 121 GYTIASSISMMAVKRSNCFHKSGGKNPCRMSSNPFMVSFGVVEIILSQIPDFDQIWWLST 180

Query: 181 VAAIMSFTYSTIGLALGIAKVAENGSFKGTVSGISVETVTQTQKIWRTFQALGDIAFAYS 240
           VAAIMSFTYSTIGLALGIAKVAENGSFKGTVSG+SV TVTQTQKIWRTFQALGDIAFAYS
Sbjct: 181 VAAIMSFTYSTIGLALGIAKVAENGSFKGTVSGMSVGTVTQTQKIWRTFQALGDIAFAYS 240

Query: 241 FSVILIEIQDTIRCPPSEAKTMKKASGFSITVTTIFYLLCGCMGYAAFGNNAPDNLLTGF 300
           FSVILIEIQDTIRCPPSEAKTMKKASGFSI VTTIFYLLCGCMGYAAFGNNAP NLLTGF
Sbjct: 241 FSVILIEIQDTIRCPPSEAKTMKKASGFSIAVTTIFYLLCGCMGYAAFGNNAPGNLLTGF 300

Query: 301 GFYNPYWLLDIANVAIVIHLVGAYQVFCQPVFAFVEKTAAQTWPDSAFITKHYKLPLSSS 360
           GFYNPYWLLDIANVAIV+HLVGAYQVFCQPVFAFVEK AAQTWPDSAFITKHYKLPLSSS
Sbjct: 301 GFYNPYWLLDIANVAIVVHLVGAYQVFCQPVFAFVEKMAAQTWPDSAFITKHYKLPLSSS 360

Query: 361 RSYNINFFRLVWRTLFVCFTTIVAMLLPFFNDIVGIMGALQFWPLTVYFPVQMYIVQKKI 420
           RSYNINFFRLVWRTLFVCFTT++AMLLPFFND+VGIMGA QFWPLTVYFPVQMYIVQKKI
Sbjct: 361 RSYNINFFRLVWRTLFVCFTTVIAMLLPFFNDVVGIMGAFQFWPLTVYFPVQMYIVQKKI 420

Query: 421 AKWSVKWVCVQTMSMGCLLISIAAGVGSLIGVVLDLKVYKPFNTRY 467
           AKWSVKWVCVQTMSMGCLLISIAAGVGSLIGVVLDLKVYKPF TRY
Sbjct: 421 AKWSVKWVCVQTMSMGCLLISIAAGVGSLIGVVLDLKVYKPFITRY 466

BLAST of CmaCh07G012680 vs. NCBI nr
Match: XP_023518210.1 (amino acid permease 2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 875.2 bits (2260), Expect = 2.6e-250
Identity = 439/466 (94.21%), Postives = 445/466 (95.49%), Query Frame = 0

Query: 1   MAVLPINDSPSFDDDGRPKRTGTFWTASAHIITAVIGSGVLSLAWAIAQLGWIAGPIVML 60
           MAVLPINDS S DDDGRPKRTGTFWTASAHIITAVIGSGVLSLAWAIAQLGWIAGPIVML
Sbjct: 1   MAVLPINDSSSSDDDGRPKRTGTFWTASAHIITAVIGSGVLSLAWAIAQLGWIAGPIVML 60

Query: 61  LFAFIGYYTSCLLVDCYRSNDSVNGKRNYTYMHAVRSFLGRGQTVACGVIQYMDLIGVAI 120
           LFAFI YYTSCLL DCYRSNDSVN KRNYTYMHAVRSFLGRGQTV CGVIQYMDLIGVAI
Sbjct: 61  LFAFISYYTSCLLTDCYRSNDSVNAKRNYTYMHAVRSFLGRGQTVVCGVIQYMDLIGVAI 120

Query: 121 GYTIASSISMMAVKRSNCFHKSGGKNPCRMSSNPFMVSFGAVEIILSQIPDFDQIWWLST 180
           GYTIASSISMMAVKRSNCFHKSGGK+PCRMSSNPFMVSFG VEIILSQIP FDQIWWLST
Sbjct: 121 GYTIASSISMMAVKRSNCFHKSGGKSPCRMSSNPFMVSFGVVEIILSQIPKFDQIWWLST 180

Query: 181 VAAIMSFTYSTIGLALGIAKVAENGSFKGTVSGISVETVTQTQKIWRTFQALGDIAFAYS 240
           VAAIMSFTYSTIGLALGIAKVAENGSFKG        TVT+TQKIWRTFQALGDIAFAYS
Sbjct: 181 VAAIMSFTYSTIGLALGIAKVAENGSFKG--------TVTETQKIWRTFQALGDIAFAYS 240

Query: 241 FSVILIEIQDTIRCPPSEAKTMKKASGFSITVTTIFYLLCGCMGYAAFGNNAPDNLLTGF 300
           FSVILIEIQDTIRCPPSEAKTMKKASGFSI VTTIFYLLCGCMGYAAFGNNAP NLLTGF
Sbjct: 241 FSVILIEIQDTIRCPPSEAKTMKKASGFSIAVTTIFYLLCGCMGYAAFGNNAPGNLLTGF 300

Query: 301 GFYNPYWLLDIANVAIVIHLVGAYQVFCQPVFAFVEKTAAQTWPDSAFITKHYKLPLSSS 360
           GFYNPYWLLDIANVAIV+HLVGAYQVFCQPVFAFVEKTAAQTWPDSAFITKHY+L LSSS
Sbjct: 301 GFYNPYWLLDIANVAIVVHLVGAYQVFCQPVFAFVEKTAAQTWPDSAFITKHYRLSLSSS 360

Query: 361 RSYNINFFRLVWRTLFVCFTTIVAMLLPFFNDIVGIMGALQFWPLTVYFPVQMYIVQKKI 420
           RSYNINFFRLVWRTLFVCFTT+VAMLLPFFNDIVGIMGA QFWPL+VYFPVQMYIVQKKI
Sbjct: 361 RSYNINFFRLVWRTLFVCFTTVVAMLLPFFNDIVGIMGAFQFWPLSVYFPVQMYIVQKKI 420

Query: 421 AKWSVKWVCVQTMSMGCLLISIAAGVGSLIGVVLDLKVYKPFNTRY 467
           AKWSVKWVCVQTMSMGCLLISIAAGVGSLIGVVLDLKVYKPF TRY
Sbjct: 421 AKWSVKWVCVQTMSMGCLLISIAAGVGSLIGVVLDLKVYKPFITRY 458

BLAST of CmaCh07G012680 vs. NCBI nr
Match: XP_022971172.1 (amino acid permease 4-like [Cucurbita maxima])

HSP 1 Score: 844.3 bits (2180), Expect = 4.9e-241
Identity = 410/466 (87.98%), Postives = 440/466 (94.42%), Query Frame = 0

Query: 1   MAVLPINDSPSFDDDGRPKRTGTFWTASAHIITAVIGSGVLSLAWAIAQLGWIAGPIVML 60
           MAVLPIND+PSFDDDGRPKRTGTFWTASAHIITAVIGSGVLSLAWAIAQLGWIAGP VML
Sbjct: 1   MAVLPINDAPSFDDDGRPKRTGTFWTASAHIITAVIGSGVLSLAWAIAQLGWIAGPSVML 60

Query: 61  LFAFIGYYTSCLLVDCYRSNDSVNGKRNYTYMHAVRSFLGRGQTVACGVIQYMDLIGVAI 120
           LFAFIGYYTSCLL DCYRS+D VNGKRNYTYMHAVRS LGRGQT ACGV+QY++LIG++I
Sbjct: 61  LFAFIGYYTSCLLADCYRSSDPVNGKRNYTYMHAVRSLLGRGQTTACGVLQYVNLIGISI 120

Query: 121 GYTIASSISMMAVKRSNCFHKSGGKNPCRMSSNPFMVSFGAVEIILSQIPDFDQIWWLST 180
           GYTIASSISMMAVKRSNCFH SGGKNPC MSSNPFMVSFG +EIILSQIPDFDQIWWLST
Sbjct: 121 GYTIASSISMMAVKRSNCFHSSGGKNPCHMSSNPFMVSFGVMEIILSQIPDFDQIWWLST 180

Query: 181 VAAIMSFTYSTIGLALGIAKVAENGSFKGTVSGISVETVTQTQKIWRTFQALGDIAFAYS 240
           VAA+MSFTYSTIGL LGIAKVAE GSFKGTVSGISV  + ++QKIWRTFQALGDIAFAYS
Sbjct: 181 VAAVMSFTYSTIGLGLGIAKVAETGSFKGTVSGISVGPINESQKIWRTFQALGDIAFAYS 240

Query: 241 FSVILIEIQDTIRCPPSEAKTMKKASGFSITVTTIFYLLCGCMGYAAFGNNAPDNLLTGF 300
           FS+ILIEIQDTIRCPPSEAKTMKKA+GFSI +TTIFY+LCGCMGYAAFGN+AP NLLTGF
Sbjct: 241 FSIILIEIQDTIRCPPSEAKTMKKATGFSIALTTIFYMLCGCMGYAAFGNDAPGNLLTGF 300

Query: 301 GFYNPYWLLDIANVAIVIHLVGAYQVFCQPVFAFVEKTAAQTWPDSAFITKHYKLPLSSS 360
           GFYNP+WLLD+ANVAIV+HLVGAYQVF QPVFAFVEK AAQ WPDS FITKH+KL +SSS
Sbjct: 301 GFYNPFWLLDVANVAIVVHLVGAYQVFSQPVFAFVEKKAAQAWPDSPFITKHHKLSISSS 360

Query: 361 RSYNINFFRLVWRTLFVCFTTIVAMLLPFFNDIVGIMGALQFWPLTVYFPVQMYIVQKKI 420
           RSYN+N FRLVWR+LFVCFTT+VAMLLPFFND+VGI+GALQFWPLTVYFPVQMYIVQKKI
Sbjct: 361 RSYNVNLFRLVWRSLFVCFTTVVAMLLPFFNDVVGIIGALQFWPLTVYFPVQMYIVQKKI 420

Query: 421 AKWSVKWVCVQTMSMGCLLISIAAGVGSLIGVVLDLKVYKPFNTRY 467
            KWS+KWVCVQTMSMGCLLIS AA VGS+IGV+LDLKVYKPF T Y
Sbjct: 421 PKWSLKWVCVQTMSMGCLLISFAAVVGSVIGVMLDLKVYKPFKTMY 466

BLAST of CmaCh07G012680 vs. TAIR 10
Match: AT5G09220.1 (amino acid permease 2 )

HSP 1 Score: 683.7 bits (1763), Expect = 1.0e-196
Identity = 326/456 (71.49%), Postives = 396/456 (86.84%), Query Frame = 0

Query: 12  FDDDGRPKRTGTFWTASAHIITAVIGSGVLSLAWAIAQLGWIAGPIVMLLFAFIGYYTSC 71
           FDDDGR KRTGT WTASAHIITAVIGSGVLSLAWAIAQLGWIAGP VMLLF+ +  Y+S 
Sbjct: 38  FDDDGRLKRTGTVWTASAHIITAVIGSGVLSLAWAIAQLGWIAGPAVMLLFSLVTLYSST 97

Query: 72  LLVDCYRSNDSVNGKRNYTYMHAVRSFLGRGQTVACGVIQYMDLIGVAIGYTIASSISMM 131
           LL DCYR+ D+V+GKRNYTYM AVRS LG  +   CG+IQY++L G+AIGYTIA+SISMM
Sbjct: 98  LLSDCYRTGDAVSGKRNYTYMDAVRSILGGFKFKICGLIQYLNLFGIAIGYTIAASISMM 157

Query: 132 AVKRSNCFHKSGGKNPCRMSSNPFMVSFGAVEIILSQIPDFDQIWWLSTVAAIMSFTYST 191
           A+KRSNCFHKSGGK+PC MSSNP+M+ FG  EI+LSQ+PDFDQIWW+S VAA+MSFTYS 
Sbjct: 158 AIKRSNCFHKSGGKDPCHMSSNPYMIVFGVAEILLSQVPDFDQIWWISIVAAVMSFTYSA 217

Query: 192 IGLALGIAKVAENGSFKGTVSGISVETVTQTQKIWRTFQALGDIAFAYSFSVILIEIQDT 251
           IGLALGI +VA NG FKG+++GIS+ TVTQTQKIWRTFQALGDIAFAYS+SV+LIEIQDT
Sbjct: 218 IGLALGIVQVAANGVFKGSLTGISIGTVTQTQKIWRTFQALGDIAFAYSYSVVLIEIQDT 277

Query: 252 IRCPPSEAKTMKKASGFSITVTTIFYLLCGCMGYAAFGNNAPDNLLTGFGFYNPYWLLDI 311
           +R PP+E+KTMKKA+  SI VTTIFY+LCG MGYAAFG+ AP NLLTGFGFYNP+WLLDI
Sbjct: 278 VRSPPAESKTMKKATKISIAVTTIFYMLCGSMGYAAFGDAAPGNLLTGFGFYNPFWLLDI 337

Query: 312 ANVAIVIHLVGAYQVFCQPVFAFVEKTAAQTWPDSAFITKHYKLPLSSSRS-YNINFFRL 371
           AN AIV+HLVGAYQVF QP+FAF+EK+ A+ +PD+ F++K +++ +   +S Y +N FR+
Sbjct: 338 ANAAIVVHLVGAYQVFAQPIFAFIEKSVAERYPDNDFLSKEFEIRIPGFKSPYKVNVFRM 397

Query: 372 VWRTLFVCFTTIVAMLLPFFNDIVGIMGALQFWPLTVYFPVQMYIVQKKIAKWSVKWVCV 431
           V+R+ FV  TT+++ML+PFFND+VGI+GAL FWPLTVYFPV+MYI Q+K+ KWS +WVC+
Sbjct: 398 VYRSGFVVTTTVISMLMPFFNDVVGILGALGFWPLTVYFPVEMYIKQRKVEKWSTRWVCL 457

Query: 432 QTMSMGCLLISIAAGVGSLIGVVLDLKVYKPFNTRY 467
           Q +S+ CL+IS+ AGVGS+ GV+LDLKVYKPF + Y
Sbjct: 458 QMLSVACLVISVVAGVGSIAGVMLDLKVYKPFKSTY 493

BLAST of CmaCh07G012680 vs. TAIR 10
Match: AT5G63850.1 (amino acid permease 4 )

HSP 1 Score: 681.4 bits (1757), Expect = 5.1e-196
Identity = 328/456 (71.93%), Postives = 389/456 (85.31%), Query Frame = 0

Query: 12  FDDDGRPKRTGTFWTASAHIITAVIGSGVLSLAWAIAQLGWIAGPIVMLLFAFIGYYTSC 71
           FDDDGR KR+GT WTASAHIITAVIGSGVLSLAWAI QLGWIAGP VMLLF+F+ YY+S 
Sbjct: 11  FDDDGRLKRSGTVWTASAHIITAVIGSGVLSLAWAIGQLGWIAGPTVMLLFSFVTYYSST 70

Query: 72  LLVDCYRSNDSVNGKRNYTYMHAVRSFLGRGQTVACGVIQYMDLIGVAIGYTIASSISMM 131
           LL DCYR+ D V+GKRNYTYM AVRS LG  +   CG+IQY++L G+ +GYTIA+SISMM
Sbjct: 71  LLSDCYRTGDPVSGKRNYTYMDAVRSILGGFRFKICGLIQYLNLFGITVGYTIAASISMM 130

Query: 132 AVKRSNCFHKSGGKNPCRMSSNPFMVSFGAVEIILSQIPDFDQIWWLSTVAAIMSFTYST 191
           A+KRSNCFH+SGGKNPC MSSNP+M+ FG  EI+LSQI DFDQIWWLS VAAIMSFTYS 
Sbjct: 131 AIKRSNCFHESGGKNPCHMSSNPYMIMFGVTEILLSQIKDFDQIWWLSIVAAIMSFTYSA 190

Query: 192 IGLALGIAKVAENGSFKGTVSGISVETVTQTQKIWRTFQALGDIAFAYSFSVILIEIQDT 251
           IGLALGI +VA NG  KG+++GIS+  VTQTQKIWRTFQALGDIAFAYS+SV+LIEIQDT
Sbjct: 191 IGLALGIIQVAANGVVKGSLTGISIGAVTQTQKIWRTFQALGDIAFAYSYSVVLIEIQDT 250

Query: 252 IRCPPSEAKTMKKASGFSITVTTIFYLLCGCMGYAAFGNNAPDNLLTGFGFYNPYWLLDI 311
           +R PP+E+KTMK A+  SI VTT FY+LCGCMGYAAFG+ AP NLLTGFGFYNP+WLLD+
Sbjct: 251 VRSPPAESKTMKIATRISIAVTTTFYMLCGCMGYAAFGDKAPGNLLTGFGFYNPFWLLDV 310

Query: 312 ANVAIVIHLVGAYQVFCQPVFAFVEKTAAQTWPDSAFITKHYKLPLSSSRS-YNINFFRL 371
           AN AIVIHLVGAYQVF QP+FAF+EK AA  +PDS  +TK Y++ +   RS Y +N FR 
Sbjct: 311 ANAAIVIHLVGAYQVFAQPIFAFIEKQAAARFPDSDLVTKEYEIRIPGFRSPYKVNVFRA 370

Query: 372 VWRTLFVCFTTIVAMLLPFFNDIVGIMGALQFWPLTVYFPVQMYIVQKKIAKWSVKWVCV 431
           V+R+ FV  TT+++ML+PFFND+VGI+GAL FWPLTVYFPV+MYI Q+K+ +WS+KWVC+
Sbjct: 371 VYRSGFVVLTTVISMLMPFFNDVVGILGALGFWPLTVYFPVEMYIRQRKVERWSMKWVCL 430

Query: 432 QTMSMGCLLISIAAGVGSLIGVVLDLKVYKPFNTRY 467
           Q +S GCL+I++ AGVGS+ GV+LDLKVYKPF T Y
Sbjct: 431 QMLSCGCLMITLVAGVGSIAGVMLDLKVYKPFKTTY 466

BLAST of CmaCh07G012680 vs. TAIR 10
Match: AT1G77380.1 (amino acid permease 3 )

HSP 1 Score: 680.6 bits (1755), Expect = 8.7e-196
Identity = 318/458 (69.43%), Postives = 391/458 (85.37%), Query Frame = 0

Query: 9   SPSFDDDGRPKRTGTFWTASAHIITAVIGSGVLSLAWAIAQLGWIAGPIVMLLFAFIGYY 68
           S   DDDG+ KRTG+ WTASAHIITAVIGSGVLSLAWA AQLGW+AGP+VMLLF+ + Y+
Sbjct: 19  SKYLDDDGKNKRTGSVWTASAHIITAVIGSGVLSLAWATAQLGWLAGPVVMLLFSAVTYF 78

Query: 69  TSCLLVDCYRSNDSVNGKRNYTYMHAVRSFLGRGQTVACGVIQYMDLIGVAIGYTIASSI 128
           TS LL  CYRS D ++GKRNYTYM AVRS LG  +   CG++QY+++ GVAIGYTIAS+I
Sbjct: 79  TSSLLAACYRSGDPISGKRNYTYMDAVRSNLGGVKVTLCGIVQYLNIFGVAIGYTIASAI 138

Query: 129 SMMAVKRSNCFHKSGGKNPCRMSSNPFMVSFGAVEIILSQIPDFDQIWWLSTVAAIMSFT 188
           SMMA+KRSNCFHKSGGK+PC M+SNP+M++FG V+I+ SQIPDFDQ+WWLS +AA+MSFT
Sbjct: 139 SMMAIKRSNCFHKSGGKDPCHMNSNPYMIAFGLVQILFSQIPDFDQLWWLSILAAVMSFT 198

Query: 189 YSTIGLALGIAKVAENGSFKGTVSGISVETVTQTQKIWRTFQALGDIAFAYSFSVILIEI 248
           YS+ GLALGIA+V  NG  KG+++GIS+  VT+TQKIWRTFQALGDIAFAYS+S+ILIEI
Sbjct: 199 YSSAGLALGIAQVVVNGKVKGSLTGISIGAVTETQKIWRTFQALGDIAFAYSYSIILIEI 258

Query: 249 QDTIRCPPSEAKTMKKASGFSITVTTIFYLLCGCMGYAAFGNNAPDNLLTGFGFYNPYWL 308
           QDT++ PPSE KTMKKA+  S++VTT+FY+LCGCMGYAAFG+ +P NLLTGFGFYNPYWL
Sbjct: 259 QDTVKSPPSEEKTMKKATLVSVSVTTMFYMLCGCMGYAAFGDLSPGNLLTGFGFYNPYWL 318

Query: 309 LDIANVAIVIHLVGAYQVFCQPVFAFVEKTAAQTWPDSAFITKHYKLPLSSSRSYNINFF 368
           LDIAN AIVIHL+GAYQV+CQP+FAF+EK A+  +PDS FI K  K+P+   +   +N F
Sbjct: 319 LDIANAAIVIHLIGAYQVYCQPLFAFIEKQASIQFPDSEFIAKDIKIPIPGFKPLRLNVF 378

Query: 369 RLVWRTLFVCFTTIVAMLLPFFNDIVGIMGALQFWPLTVYFPVQMYIVQKKIAKWSVKWV 428
           RL+WRT+FV  TT+++MLLPFFND+VG++GAL FWPLTVYFPV+MYI QKKI +WS +WV
Sbjct: 379 RLIWRTVFVIITTVISMLLPFFNDVVGLLGALGFWPLTVYFPVEMYIAQKKIPRWSTRWV 438

Query: 429 CVQTMSMGCLLISIAAGVGSLIGVVLDLKVYKPFNTRY 467
           C+Q  S+GCL++SIAA  GS+ GV+LDLK YKPF + Y
Sbjct: 439 CLQVFSLGCLVVSIAAAAGSIAGVLLDLKSYKPFRSEY 476

BLAST of CmaCh07G012680 vs. TAIR 10
Match: AT1G44100.1 (amino acid permease 5 )

HSP 1 Score: 641.3 bits (1653), Expect = 5.8e-184
Identity = 300/470 (63.83%), Postives = 383/470 (81.49%), Query Frame = 0

Query: 3   VLPINDSPSFDDDGRPKRTGTFWTASAHIITAVIGSGVLSLAWAIAQLGWIAGPIVMLLF 62
           VLP + S SFDDDGRPKRTGT WTASAHIITAVIGSGVLSLAWA+AQ+GWI GP+ MLLF
Sbjct: 11  VLPKHSSDSFDDDGRPKRTGTVWTASAHIITAVIGSGVLSLAWAVAQIGWIGGPVAMLLF 70

Query: 63  AFIGYYTSCLLVDCYRSNDSVNGKRNYTYMHAVRSFLGRGQTVACGVIQYMDLIGVAIGY 122
           +F+ +YTS LL  CYRS DSV GKRNYTYM A+ S LG  +   CGV+QY++L G AIGY
Sbjct: 71  SFVTFYTSTLLCSCYRSGDSVTGKRNYTYMDAIHSNLGGIKVKVCGVVQYVNLFGTAIGY 130

Query: 123 TIASSISMMAVKRSNCFHKSGGKNPCRMSSNPFMVSFGAVEIILSQIPDFDQIWWLSTVA 182
           TIAS+IS++A++R++C   +G  +PC ++ N +M++FG V+II SQIPDFDQ+WWLS VA
Sbjct: 131 TIASAISLVAIQRTSCQQMNGPNDPCHVNGNVYMIAFGIVQIIFSQIPDFDQLWWLSIVA 190

Query: 183 AIMSFTYSTIGLALGIAKVAENGSFKGTVSGISV------ETVTQTQKIWRTFQALGDIA 242
           A+MSF YS IGL LG++KV EN   KG+++G++V       TVT +QKIWRTFQ+LG+IA
Sbjct: 191 AVMSFAYSAIGLGLGVSKVVENKEIKGSLTGVTVGTVTLSGTVTSSQKIWRTFQSLGNIA 250

Query: 243 FAYSFSVILIEIQDTIRCPPSEAKTMKKASGFSITVTTIFYLLCGCMGYAAFGNNAPDNL 302
           FAYS+S+ILIEIQDT++ PP+E  TM+KA+  S+ VTT+FY+LCGC+GYAAFG+NAP NL
Sbjct: 251 FAYSYSMILIEIQDTVKSPPAEVNTMRKATFVSVAVTTVFYMLCGCVGYAAFGDNAPGNL 310

Query: 303 LTGFGFYNPYWLLDIANVAIVIHLVGAYQVFCQPVFAFVEKTAAQTWPDSAFITKHYKLP 362
           L   GF NPYWLLDIAN+AIVIHLVGAYQV+CQP+FAFVEK A++ +P+S F+TK  K+ 
Sbjct: 311 LAHGGFRNPYWLLDIANLAIVIHLVGAYQVYCQPLFAFVEKEASRRFPESEFVTKEIKIQ 370

Query: 363 LSSSRSYNINFFRLVWRTLFVCFTTIVAMLLPFFNDIVGIMGALQFWPLTVYFPVQMYIV 422
           L   + +N+N FRLVWRT FV  TT+++ML+PFFND+VG++GA+ FWPLTVYFPV+MYI 
Sbjct: 371 LFPGKPFNLNLFRLVWRTFFVMTTTLISMLMPFFNDVVGLLGAIGFWPLTVYFPVEMYIA 430

Query: 423 QKKIAKWSVKWVCVQTMSMGCLLISIAAGVGSLIGVVLDLKVYKPFNTRY 467
           QK + +W  KWVC+Q +S+ CL +S+AA  GS+IG+V DLKVYKPF + +
Sbjct: 431 QKNVPRWGTKWVCLQVLSVTCLFVSVAAAAGSVIGIVSDLKVYKPFQSEF 480

BLAST of CmaCh07G012680 vs. TAIR 10
Match: AT5G49630.1 (amino acid permease 6 )

HSP 1 Score: 561.6 bits (1446), Expect = 5.9e-160
Identity = 270/455 (59.34%), Postives = 349/455 (76.70%), Query Frame = 0

Query: 11  SFDDDGRPKRTGTFWTASAHIITAVIGSGVLSLAWAIAQLGWIAGPIVMLLFAFIGYYTS 70
           +FD+DGR KRTGT+ T SAHIITAVIGSGVLSLAWAIAQLGW+AGP V++ F+FI Y+TS
Sbjct: 24  NFDEDGRDKRTGTWMTGSAHIITAVIGSGVLSLAWAIAQLGWVAGPAVLMAFSFITYFTS 83

Query: 71  CLLVDCYRSNDSVNGKRNYTYMHAVRSFLGRGQTVACGVIQYMDLIGVAIGYTIASSISM 130
            +L DCYRS D V GKRNYTYM  VRS+LG  +   CG+ QY +LIG+ IGYTI +SISM
Sbjct: 84  TMLADCYRSPDPVTGKRNYTYMEVVRSYLGGRKVQLCGLAQYGNLIGITIGYTITASISM 143

Query: 131 MAVKRSNCFHKSGGKNPCRMSSNPFMVSFGAVEIILSQIPDFDQIWWLSTVAAIMSFTYS 190
           +AVKRSNCFHK+G    C  S+ PFM+ F  ++IILSQIP+F  + WLS +AA+MSF Y+
Sbjct: 144 VAVKRSNCFHKNGHNVKCATSNTPFMIIFAIIQIILSQIPNFHNLSWLSILAAVMSFCYA 203

Query: 191 TIGLALGIAKVAENGS-FKGTVSGISVE-TVTQTQKIWRTFQALGDIAFAYSFSVILIEI 250
           +IG+ L IAK A  G   + T++G++V   V+  +KIWRTFQA+GDIAFAY++S +LIEI
Sbjct: 204 SIGVGLSIAKAAGGGEHVRTTLTGVTVGIDVSGAEKIWRTFQAIGDIAFAYAYSTVLIEI 263

Query: 251 QDTIRC-PPSEAKTMKKASGFSITVTTIFYLLCGCMGYAAFGNNAPDNLLTGFGFYNPYW 310
           QDT++  PPSE K MK+AS   ++ TT FY+LCGC+GYAAFGN+AP N LTGFGFY P+W
Sbjct: 264 QDTLKAGPPSENKAMKRASLVGVSTTTFFYMLCGCVGYAAFGNDAPGNFLTGFGFYEPFW 323

Query: 311 LLDIANVAIVIHLVGAYQVFCQPVFAFVEKTAAQTWPDSAFITKHYKLPLSSSRSYNINF 370
           L+D ANV I +HL+GAYQVFCQP+F FVE  +A+ WPD+ FIT  YK+ +     ++INF
Sbjct: 324 LIDFANVCIAVHLIGAYQVFCQPIFQFVESQSAKRWPDNKFITGEYKIHVPCCGDFSINF 383

Query: 371 FRLVWRTLFVCFTTIVAMLLPFFNDIVGIMGALQFWPLTVYFPVQMYIVQKKIAKWSVKW 430
            RLVWRT +V  T +VAM+ PFFND +G++GA  FWPLTVYFP++M+I QKKI K+S  W
Sbjct: 384 LRLVWRTSYVVVTAVVAMIFPFFNDFLGLIGAASFWPLTVYFPIEMHIAQKKIPKFSFTW 443

Query: 431 VCVQTMSMGCLLISIAAGVGSLIGVVLDLKVYKPF 463
             ++ +S  C ++S+ A  GS+ G++  LK +KPF
Sbjct: 444 TWLKILSWTCFIVSLVAAAGSVQGLIQSLKDFKPF 478

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q389671.4e-19571.49Amino acid permease 2 OS=Arabidopsis thaliana OX=3702 GN=AAP2 PE=1 SV=1[more]
Q9FN047.2e-19571.93Amino acid permease 4 OS=Arabidopsis thaliana OX=3702 GN=AAP4 PE=1 SV=1[more]
Q391341.2e-19469.43Amino acid permease 3 OS=Arabidopsis thaliana OX=3702 GN=AAP3 PE=1 SV=2[more]
Q8GUM38.2e-18363.83Amino acid permease 5 OS=Arabidopsis thaliana OX=3702 GN=AAP5 PE=1 SV=1[more]
P929348.3e-15959.34Amino acid permease 6 OS=Arabidopsis thaliana OX=3702 GN=AAP6 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1HRN43.9e-268100.00amino acid permease 4-like OS=Cucurbita maxima OX=3661 GN=LOC111466004 PE=3 SV=1[more]
A0A6J1EAD39.5e-25995.92amino acid permease 4-like OS=Cucurbita moschata OX=3662 GN=LOC111432325 PE=3 SV... [more]
A0A6J1I1842.4e-24187.98amino acid permease 4-like OS=Cucurbita maxima OX=3661 GN=LOC111469952 PE=3 SV=1[more]
A0A6J1EXV81.7e-23986.70amino acid permease 4-like OS=Cucurbita moschata OX=3662 GN=LOC111439300 PE=3 SV... [more]
A0A6J1DSY48.7e-23686.70amino acid permease 4-like OS=Momordica charantia OX=3673 GN=LOC111023641 PE=3 S... [more]
Match NameE-valueIdentityDescription
XP_022966303.18.0e-268100.00amino acid permease 4-like [Cucurbita maxima][more]
KAG6595602.11.2e-25896.35Amino acid permease 2, partial [Cucurbita argyrosperma subsp. sororia] >KAG70275... [more]
XP_022924922.12.0e-25895.92amino acid permease 4-like [Cucurbita moschata][more]
XP_023518210.12.6e-25094.21amino acid permease 2-like [Cucurbita pepo subsp. pepo][more]
XP_022971172.14.9e-24187.98amino acid permease 4-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
AT5G09220.11.0e-19671.49amino acid permease 2 [more]
AT5G63850.15.1e-19671.93amino acid permease 4 [more]
AT1G77380.18.7e-19669.43amino acid permease 3 [more]
AT1G44100.15.8e-18463.83amino acid permease 5 [more]
AT5G49630.15.9e-16059.34amino acid permease 6 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR013057Amino acid transporter, transmembrane domainPFAMPF01490Aa_transcoord: 20..453
e-value: 8.1E-112
score: 373.9
NoneNo IPR availablePANTHERPTHR48017OS05G0424000 PROTEIN-RELATEDcoord: 9..460
NoneNo IPR availablePANTHERPTHR48017:SF167OS02G0102200 PROTEINcoord: 9..460

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh07G012680.1CmaCh07G012680.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006865 amino acid transport
biological_process GO:0009734 auxin-activated signaling pathway
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005886 plasma membrane
molecular_function GO:0015293 symporter activity