CmaCh07G012140 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh07G012140
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Descriptionheparanase-like protein 1
LocationCma_Chr07: 6669754 .. 6671810 (-)
RNA-Seq ExpressionCmaCh07G012140
SyntenyCmaCh07G012140
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
GACTTAACTCTTCCTATCATGAACAAAGCCGTTCAAGGTTTGAGTATTACCAATGCATAAATGTTTCTTCTTTACGACATTCCGCTAAAAATCTAACCTAATTTGCCTTCTACCATTAGCTTTCAAGTCGGTAAGGATTAGAGTTGGAGGCACTTTACAAGACAAATTGATTTACAACGTTGGGGCTGGCTTCCAGGGAACCTGTCACCCATTTCAAGCAACCAAAGGTTCGTTGTTTGACTTCTCGGCGGGATGTTTGTACATGGAAAGGTGGGACGATTTGAACAACTTTTTCAACAATACCGGGTATGTTATTTTTCTTTTGTGTTTTTTTTCTTACTTTCTATTTTCCGCTCCTGTACTAAATATATATATTTTAATATAACAGGGCGATTGTGACTTTTGGCTTAAACGCTCTCTTGGGTAAGTACAAGACGCAAGGAATTCAATGGGAAGGAAATTGGAACTATAGCAATGCCGAGGCTCTTATTCAATACACGGTAGAGAATAATTATCAAATAAATTCATGGGAGTTTGGTAAGTTAAATCATTCCATCACTCTTGGTTGACGTAACAACTTTAGTTCATGCTCACTGAGGCGTATGCCACTACACAGGCAATGAACTGGCTGGAATTAATAGCATTGGTGCAAGTGTTACTGCTGCACAATATGCAAAAGACGTCATCAAGCTTCGAGAAATCATAGATCGTTTGTACAATAACTCCCAACAAAAACCTATGATTGTAGCACCTGGTGCATTCTTTGATGAGAAATGGTATGCGGAATTTCTTAGAAAAACTGGACCAGGAGTTGTCGACGTTCTCACTCATCATATATACAACATGGGTGCAGGTTTGGCTGCAATACTTACTACCATCCTTCATTATGTGTTTTATATATAAATTCTTACTACGAGTATTTAGAGTGTGTTTTTGGTTTGTAGGTGATGACCCCAAATTGGTTTATAGGTTCGTTAATTCAACGTATCTTTCTCAAGTATCGAACACATTTGAACATCTCGAGAGCGTGATTCAAAAGAATGCCCCTTGGGGTGCCGCTTGGGTTGGAGAAGCTGGTGGAGCTTATCAGGGTGGCAGTCCTCATATTTCTTATACATTTATCAATAGTTTTTGGTAATCAAATCTATTTAGACTTTTTTTCCCTCTATATTTGATTTGTCTCCACTTAAACATGAAGAAAAGAACAATGCTCGAGTACTTACAGAATGAATTGAATGCATTAGGTACTTGGATCAGCTCGGGATGGCTTCAATGTACAATACAAAAGTATATTGTCGACAGACTTTGATCGGTGGATTCTATGGTGTCATCAAATCCTCAACTTTACTTCCTACGCCAGATTACTATGGGTAAGATCGGTGGATTCTATGGTGTCATCAAATCCTCAACTTTACTTCCTACGCCAGATTACTATGGGTAACCCTTTCTAATTTATAAAAATTATATCACTTCTTCTCCCGTTTTCAAATCATCTAATCTTTAAACAAAGTTCGATATTTTCTTTTTTTTTTATTAGTGCACTTCTCTTTCATCGACTCATGGGACCCCGTGTTCTCAAAATTGACAATAATGTCTCTTCTGACCTTCGTAGCTATGCCCATTGCTCGAGAGGAAGAGTAAGAAGTTGCTTTTAGATTTATATAAATTAAAACTTTATTTGATACCATTTATTGATCCTAATTTATCATTTTATGTAGTCTGGTGTAACCTTGCTTTTCATCAATTTGAGCAATGCAACCGACTTTATAATTGACCTTGAAAACAACATCAACTTGAGTTCAGGTAAGAGAAACGCTTCAAAAGGTCAAAGAGTGAAAATAGATTCATCAAGAGAGGAATACCATTTGACTCCGAAAGATGGTCTAGTTAGAAGTTCCACGGTGCTTTTAAATGGAAATCCATTGGAGACTACCAAAGAGGGAGACATACCAAATCTTATACCTGTCTACCGTCAGAGCAACTCTTCTATACATATTGCTGGTTGGTCCATTGCTTTCATTGTCGTCCCTCACTTTGTAGCTCCAGCATGCAACTAA

mRNA sequence

GACTTAACTCTTCCTATCATGAACAAAGCCGTTCAAGCTTTCAAGTCGGTAAGGATTAGAGTTGGAGGCACTTTACAAGACAAATTGATTTACAACGTTGGGGCTGGCTTCCAGGGAACCTGTCACCCATTTCAAGCAACCAAAGGTTCGTTGTTTGACTTCTCGGCGGGATGTTTGTACATGGAAAGGTGGGACGATTTGAACAACTTTTTCAACAATACCGGGGCGATTGTGACTTTTGGCTTAAACGCTCTCTTGGGTAAGTACAAGACGCAAGGAATTCAATGGGAAGGAAATTGGAACTATAGCAATGCCGAGGCTCTTATTCAATACACGGTAGAGAATAATTATCAAATAAATTCATGGGAGTTTGGCAATGAACTGGCTGGAATTAATAGCATTGGTGCAAGTGTTACTGCTGCACAATATGCAAAAGACGTCATCAAGCTTCGAGAAATCATAGATCGTTTGTACAATAACTCCCAACAAAAACCTATGATTGTAGCACCTGGTGCATTCTTTGATGAGAAATGGTATGCGGAATTTCTTAGAAAAACTGGACCAGGAGTTGTCGACGTTCTCACTCATCATATATACAACATGGGTGCAGGTGATGACCCCAAATTGGTTTATAGGTTCGTTAATTCAACGTATCTTTCTCAAGTATCGAACACATTTGAACATCTCGAGAGCGTGATTCAAAAGAATGCCCCTTGGGGTGCCGCTTGGGTTGGAGAAGCTGGTGGAGCTTATCAGGGTGGCAGTCCTCATATTTCTTATACATTTATCAATAGTTTTTGGTACTTGGATCAGCTCGGGATGGCTTCAATGTACAATACAAAAGTATATTGTCGACAGACTTTGATCGGTGGATTCTATGGTGTCATCAAATCCTCAACTTTACTTCCTACGCCAGATTACTATGGTGCACTTCTCTTTCATCGACTCATGGGACCCCGTGTTCTCAAAATTGACAATAATGTCTCTTCTGACCTTCGTAGCTATGCCCATTGCTCGAGAGGAAGATCTGGTGTAACCTTGCTTTTCATCAATTTGAGCAATGCAACCGACTTTATAATTGACCTTGAAAACAACATCAACTTGAGTTCAGGTAAGAGAAACGCTTCAAAAGGTCAAAGAGTGAAAATAGATTCATCAAGAGAGGAATACCATTTGACTCCGAAAGATGGTCTAGTTAGAAGTTCCACGGTGCTTTTAAATGGAAATCCATTGGAGACTACCAAAGAGGGAGACATACCAAATCTTATACCTGTCTACCGTCAGAGCAACTCTTCTATACATATTGCTGGTTGGTCCATTGCTTTCATTGTCGTCCCTCACTTTGTAGCTCCAGCATGCAACTAA

Coding sequence (CDS)

GACTTAACTCTTCCTATCATGAACAAAGCCGTTCAAGCTTTCAAGTCGGTAAGGATTAGAGTTGGAGGCACTTTACAAGACAAATTGATTTACAACGTTGGGGCTGGCTTCCAGGGAACCTGTCACCCATTTCAAGCAACCAAAGGTTCGTTGTTTGACTTCTCGGCGGGATGTTTGTACATGGAAAGGTGGGACGATTTGAACAACTTTTTCAACAATACCGGGGCGATTGTGACTTTTGGCTTAAACGCTCTCTTGGGTAAGTACAAGACGCAAGGAATTCAATGGGAAGGAAATTGGAACTATAGCAATGCCGAGGCTCTTATTCAATACACGGTAGAGAATAATTATCAAATAAATTCATGGGAGTTTGGCAATGAACTGGCTGGAATTAATAGCATTGGTGCAAGTGTTACTGCTGCACAATATGCAAAAGACGTCATCAAGCTTCGAGAAATCATAGATCGTTTGTACAATAACTCCCAACAAAAACCTATGATTGTAGCACCTGGTGCATTCTTTGATGAGAAATGGTATGCGGAATTTCTTAGAAAAACTGGACCAGGAGTTGTCGACGTTCTCACTCATCATATATACAACATGGGTGCAGGTGATGACCCCAAATTGGTTTATAGGTTCGTTAATTCAACGTATCTTTCTCAAGTATCGAACACATTTGAACATCTCGAGAGCGTGATTCAAAAGAATGCCCCTTGGGGTGCCGCTTGGGTTGGAGAAGCTGGTGGAGCTTATCAGGGTGGCAGTCCTCATATTTCTTATACATTTATCAATAGTTTTTGGTACTTGGATCAGCTCGGGATGGCTTCAATGTACAATACAAAAGTATATTGTCGACAGACTTTGATCGGTGGATTCTATGGTGTCATCAAATCCTCAACTTTACTTCCTACGCCAGATTACTATGGTGCACTTCTCTTTCATCGACTCATGGGACCCCGTGTTCTCAAAATTGACAATAATGTCTCTTCTGACCTTCGTAGCTATGCCCATTGCTCGAGAGGAAGATCTGGTGTAACCTTGCTTTTCATCAATTTGAGCAATGCAACCGACTTTATAATTGACCTTGAAAACAACATCAACTTGAGTTCAGGTAAGAGAAACGCTTCAAAAGGTCAAAGAGTGAAAATAGATTCATCAAGAGAGGAATACCATTTGACTCCGAAAGATGGTCTAGTTAGAAGTTCCACGGTGCTTTTAAATGGAAATCCATTGGAGACTACCAAAGAGGGAGACATACCAAATCTTATACCTGTCTACCGTCAGAGCAACTCTTCTATACATATTGCTGGTTGGTCCATTGCTTTCATTGTCGTCCCTCACTTTGTAGCTCCAGCATGCAACTAA

Protein sequence

DLTLPIMNKAVQAFKSVRIRVGGTLQDKLIYNVGAGFQGTCHPFQATKGSLFDFSAGCLYMERWDDLNNFFNNTGAIVTFGLNALLGKYKTQGIQWEGNWNYSNAEALIQYTVENNYQINSWEFGNELAGINSIGASVTAAQYAKDVIKLREIIDRLYNNSQQKPMIVAPGAFFDEKWYAEFLRKTGPGVVDVLTHHIYNMGAGDDPKLVYRFVNSTYLSQVSNTFEHLESVIQKNAPWGAAWVGEAGGAYQGGSPHISYTFINSFWYLDQLGMASMYNTKVYCRQTLIGGFYGVIKSSTLLPTPDYYGALLFHRLMGPRVLKIDNNVSSDLRSYAHCSRGRSGVTLLFINLSNATDFIIDLENNINLSSGKRNASKGQRVKIDSSREEYHLTPKDGLVRSSTVLLNGNPLETTKEGDIPNLIPVYRQSNSSIHIAGWSIAFIVVPHFVAPACN
Homology
BLAST of CmaCh07G012140 vs. ExPASy Swiss-Prot
Match: Q9FF10 (Heparanase-like protein 1 OS=Arabidopsis thaliana OX=3702 GN=At5g07830 PE=2 SV=1)

HSP 1 Score: 517.3 bits (1331), Expect = 1.7e-145
Identity = 243/470 (51.70%), Postives = 339/470 (72.13%), Query Frame = 0

Query: 1   DLTLPIMNKAVQAFKSVRIRVGGTLQDKLIYNVGAGFQGTCHPFQATKGSLFDFSAGCLY 60
           DLT P++ KA++AFK +RIR+GG+LQD++IY+VG   +  C PFQ     LF FS GCL+
Sbjct: 76  DLTRPLLTKAIKAFKPLRIRIGGSLQDQVIYDVG-NLKTPCRPFQKMNSGLFGFSKGCLH 135

Query: 61  MERWDDLNNFFNNTGAIVTFGLNALLGKYKTQGIQWEGNWNYSNAEALIQYTVENNYQIN 120
           M+RWD+LN+F   TGA+VTFGLNAL G++K +G  W G W++ N +  + YTV   Y I+
Sbjct: 136 MKRWDELNSFLTATGAVVTFGLNALRGRHKLRGKAWGGAWDHINTQDFLNYTVSKGYVID 195

Query: 121 SWEFGNELAGINSIGASVTAAQYAKDVIKLREIIDRLYNNS-QQKPMIVAPGAFFDEKWY 180
           SWEFGNEL+G + +GASV+A  Y KD+I L+++I+++Y NS   KP++VAPG F++++WY
Sbjct: 196 SWEFGNELSG-SGVGASVSAELYGKDLIVLKDVINKVYKNSWLHKPILVAPGGFYEQQWY 255

Query: 181 AEFLRKTGPGVVDVLTHHIYNMGAGDDPKLVYRFVNSTYLSQVSNTFEHLESVIQKNAPW 240
            + L  +GP VVDV+THHIYN+G+G+DP LV + ++ +YLSQVS TF+ +   IQ++ PW
Sbjct: 256 TKLLEISGPSVVDVVTHHIYNLGSGNDPALVKKIMDPSYLSQVSKTFKDVNQTIQEHGPW 315

Query: 241 GAAWVGEAGGAYQGGSPHISYTFINSFWYLDQLGMASMYNTKVYCRQTLIGGFYGVIKSS 300
            + WVGE+GGAY  G  H+S TFI+SFWYLDQLGM++ +NTKVYCRQTL+GGFYG+++  
Sbjct: 316 ASPWVGESGGAYNSGGRHVSDTFIDSFWYLDQLGMSARHNTKVYCRQTLVGGFYGLLEKG 375

Query: 301 TLLPTPDYYGALLFHRLMGPRVLKIDNNVSSDLRSYAHCSRGRSGVTLLFINLSNATDFI 360
           T +P PDYY ALL+HRLMG  VL +  +    LR YAHCS+GR+GVTLL INLSN +DF 
Sbjct: 376 TFVPNPDYYSALLWHRLMGKGVLAVQTDGPPQLRVYAHCSKGRAGVTLLLINLSNQSDFT 435

Query: 361 IDLENNINL---SSGKRNASKGQRVKIDSS------------REEYHLTPKDGLVRSSTV 420
           + + N IN+   +  ++  S    +K   S            REEYHLTP++G++RS T+
Sbjct: 436 VSVSNGINVVLNAESRKKKSLLDTLKRPFSWIGSKASDGYLNREEYHLTPENGVLRSKTM 495

Query: 421 LLNGNPLETTKEGDIPNLIPVYRQSNSSIHIAGWSIAFIVVPHFVAPACN 455
           +LNG  L+ T  GDIP+L PV R  NS +++   S++FIV+P+F A AC+
Sbjct: 496 VLNGKSLKPTATGDIPSLEPVLRSVNSPLNVLPLSMSFIVLPNFDASACS 543

BLAST of CmaCh07G012140 vs. ExPASy Swiss-Prot
Match: Q8L608 (Heparanase-like protein 2 OS=Arabidopsis thaliana OX=3702 GN=At5g61250 PE=2 SV=1)

HSP 1 Score: 508.8 bits (1309), Expect = 6.2e-143
Identity = 246/469 (52.45%), Postives = 329/469 (70.15%), Query Frame = 0

Query: 1   DLTLPIMNKAVQAFKSVRIRVGGTLQDKLIYNVGAGFQGTCHPFQATKGSLFDFSAGCLY 60
           +L  P++ KA+QAF+++RIR+GG+LQD++IY+VG   +  C  F+ T   LF FS GCLY
Sbjct: 73  NLASPLLAKAIQAFRTLRIRIGGSLQDQVIYDVG-DLKTPCTQFKKTDDGLFGFSEGCLY 132

Query: 61  MERWDDLNNFFNNTGAIVTFGLNALLGKYKTQGIQWEGNWNYSNAEALIQYTVENNYQIN 120
           M+RWD++N+FFN TGAIVTFGLNAL G+ K  G  W G+W+++N +  + YTV   Y I+
Sbjct: 133 MKRWDEVNHFFNATGAIVTFGLNALHGRNKLNGTAWGGDWDHTNTQDFMNYTVSKGYAID 192

Query: 121 SWEFGNELAGINSIGASVTAAQYAKDVIKLREIIDRLYNNSQQKPMIVAPGAFFDEKWYA 180
           SWEFGNEL+G + I ASV+   Y KD+I L+ +I  +Y NS+ KP++VAPG FF+E+WY+
Sbjct: 193 SWEFGNELSG-SGIWASVSVELYGKDLIVLKNVIKNVYKNSRTKPLVVAPGGFFEEQWYS 252

Query: 181 EFLRKTGPGVVDVLTHHIYNMGAGDDPKLVYRFVNSTYLSQVSNTFEHLESVIQKNAPWG 240
           E LR +GPGV+DVLTHHIYN+G G+DPKLV + ++  YLS +S  F ++   IQ++ PW 
Sbjct: 253 ELLRLSGPGVLDVLTHHIYNLGPGNDPKLVNKILDPNYLSGISELFANVNQTIQEHGPWA 312

Query: 241 AAWVGEAGGAYQGGSPHISYTFINSFWYLDQLGMASMYNTKVYCRQTLIGGFYGVIKSST 300
           AAWVGEAGGA+  G   +S TFINSFWYLDQLG++S +NTKVYCRQ L+GGFYG+++  T
Sbjct: 313 AAWVGEAGGAFNSGGRQVSETFINSFWYLDQLGISSKHNTKVYCRQALVGGFYGLLEKET 372

Query: 301 LLPTPDYYGALLFHRLMGPRVLKIDNNVSSDLRSYAHCSRGRSGVTLLFINLSNATDFII 360
            +P PDYY ALL+HRLMG  +L +    S  LR+Y HCS+ R+G+T+L INLS  T F +
Sbjct: 373 FVPNPDYYSALLWHRLMGKGILGVQTTASEYLRAYVHCSKRRAGITILLINLSKHTTFTV 432

Query: 361 DLENNINL----SSGKRNA------SKGQRVKIDSS-----REEYHLTPKDGLVRSSTVL 420
            + N + +     S KR +      SK   V   +S     REEYHL+PKDG +RS  +L
Sbjct: 433 AVSNGVKVVLQAESMKRKSFLETIKSKVSWVGNKASDGYLNREEYHLSPKDGDLRSKIML 492

Query: 421 LNGNPLETTKEGDIPNLIPVYRQSNSSIHIAGWSIAFIVVPHFVAPACN 455
           LNG PL  T  GDIP L PV     S ++I   SI+FIV+P F APAC+
Sbjct: 493 LNGKPLVPTATGDIPKLEPVRHGVKSPVYINPLSISFIVLPTFDAPACS 539

BLAST of CmaCh07G012140 vs. ExPASy Swiss-Prot
Match: Q9FZP1 (Heparanase-like protein 3 OS=Arabidopsis thaliana OX=3702 GN=At5g34940 PE=2 SV=2)

HSP 1 Score: 405.2 bits (1040), Expect = 9.6e-112
Identity = 206/464 (44.40%), Postives = 289/464 (62.28%), Query Frame = 0

Query: 1   DLTLPIMNKAVQAFKSVRIRVGGTLQDKLIYNVGAGFQGTCHPFQATKGSLFDFSAGCLY 60
           DL   I+  A++AF  ++IR+GGTLQD +IY      Q  C PF      LF ++ GCL 
Sbjct: 77  DLNNVILQNAIKAFAPLKIRIGGTLQDIVIYETPDSKQ-PCLPFTKNSSILFGYTQGCLP 136

Query: 61  MERWDDLNNFFNNTGAIVTFGLNALLGKYKTQGIQWEGNWNYSNAEALIQYTVENNYQIN 120
           M RWD+LN FF  TG  V FGLNAL G+      +  G WNY+NAE+ I++T ENNY I+
Sbjct: 137 MRRWDELNAFFRKTGTKVIFGLNALSGRSIKSNGEAIGAWNYTNAESFIRFTAENNYTID 196

Query: 121 SWEFGNELAGINSIGASVTAAQYAKDVIKLREIIDRLYNNSQQKPMIVAPGAFFDEKWYA 180
            WE GNEL G + +GA V A QYA D I LR I++R+Y N    P+++ PG FF+  W+ 
Sbjct: 197 GWELGNELCG-SGVGARVGANQYAIDTINLRNIVNRVYKNVSPMPLVIGPGGFFEVDWFT 256

Query: 181 EFLRKTGPGVVDVLTHHIYNMGAGDDPKLVYRFVNSTYLSQVSNTFEHLESVIQKNAPWG 240
           E+L K     ++  T HIY++G G D  L+ + +N +YL Q + +F  L+++I+ ++   
Sbjct: 257 EYLNK-AENSLNATTRHIYDLGPGVDEHLIEKILNPSYLDQEAKSFRSLKNIIKNSSTKA 316

Query: 241 AAWVGEAGGAYQGGSPHISYTFINSFWYLDQLGMASMYNTKVYCRQTLIGGFYGVIKSST 300
            AWVGE+GGAY  G   +S  F+ SFWYLDQLGMAS+Y+TK YCRQ+LIGG YG++ ++ 
Sbjct: 317 VAWVGESGGAYNSGRNLVSNAFVYSFWYLDQLGMASLYDTKTYCRQSLIGGNYGLLNTTN 376

Query: 301 LLPTPDYYGALLFHRLMGPRVLKIDNNVSSDLRSYAHCSRGRSGVTLLFINLSNATDFI- 360
             P PDYY AL++ +LMG + L    + +  +RSY HC+R   G+T+L +NL N T  + 
Sbjct: 377 FTPNPDYYSALIWRQLMGRKALFTTFSGTKKIRSYTHCARQSKGITVLLMNLDNTTTVVA 436

Query: 361 -IDLENNINL------SSGKRNASK---GQRVKIDSSREEYHLTPKDGLVRSSTVLLNGN 420
            ++L N+ +L       S KR +S+   G    I   REEYHLT KDG + S T+LLNGN
Sbjct: 437 KVELNNSFSLRHTKHMKSYKRASSQLFGGPNGVI--QREEYHLTAKDGNLHSQTMLLNGN 496

Query: 421 PLETTKEGDIPNLIPVYRQSNSSIHIAGWSIAFIVVPHFVAPAC 454
            L+    GD+P + P++  S   I IA +SI F+ + + V PAC
Sbjct: 497 ALQVNSMGDLPPIEPIHINSTEPITIAPYSIVFVHMRNVVVPAC 535

BLAST of CmaCh07G012140 vs. ExPASy Swiss-Prot
Match: Q9LRC8 (Baicalin-beta-D-glucuronidase OS=Scutellaria baicalensis OX=65409 GN=SGUS PE=1 SV=1)

HSP 1 Score: 330.1 bits (845), Expect = 3.9e-89
Identity = 171/460 (37.17%), Postives = 262/460 (56.96%), Query Frame = 0

Query: 1   DLTLPIMNKAVQAFKSVRIRVGGTLQDKLIYNVGAGFQGTCHPFQATKGSLFDFSAGCLY 60
           DL   I+  AV+ F  +++R GGTLQD+L+Y      +     F      + DFS  CL 
Sbjct: 74  DLNNNIIRNAVKEFAPLKLRFGGTLQDRLVYQTSRD-EPCDSTFYNNTNLILDFSHACLS 133

Query: 61  MERWDDLNNFFNNTGAIVTFGLNALLGK------------YKTQGIQWEGNWNYSNAEAL 120
           ++RWD++N F   TG+   FGLNAL GK            Y  +     G W+YSN++ L
Sbjct: 134 LDRWDEINQFILETGSEAVFGLNALRGKTVEIKGIIKDGQYLGETTTAVGEWDYSNSKFL 193

Query: 121 IQYTVENNYQ-INSWEFGNELAGINSIGASVTAAQYAKDVIKLREIIDRLYNNSQQKPMI 180
           I+Y+++  Y+ I  W  GNEL G +++   V+   YA D  KL E++  +Y +    P+I
Sbjct: 194 IEYSLKKGYKHIRGWTLGNELGG-HTLFIGVSPEDYANDAKKLHELVKEIYQDQGTMPLI 253

Query: 181 VAPGAFFDEKWYAEFLRKTGPGVVDVLTHHIYNMGAGDDPKLVYRFVNSTYLSQVSNT-F 240
           +APGA FD +WY EF+ +T    + V THH+YN+G+G D  L    + +++  + + + +
Sbjct: 254 IAPGAIFDLEWYTEFIDRTPE--LHVATHHMYNLGSGGDDALKDVLLTASFFDEATKSMY 313

Query: 241 EHLESVIQKNAPWGAAWVGEAGGAYQGGSPHISYTFINSFWYLDQLGMASMYNTKVYCRQ 300
           E L+ ++ +      AW+GEAGGA+  G   IS TFIN FWYL+ LG +++ +TK +CRQ
Sbjct: 314 EGLQKIVNRPGTKAVAWIGEAGGAFNSGQDGISNTFINGFWYLNMLGYSALLDTKTFCRQ 373

Query: 301 TLIGGFYGVIKSSTLLPTPDYYGALLFHRLMGPRVLKIDNNVSSDLRSYAHCSRGRSGVT 360
           TL GG YG++++ T +P PDYY ALL+HRLMG +VLK +   + ++  YAHC++  +G+T
Sbjct: 374 TLTGGNYGLLQTGTYIPNPDYYSALLWHRLMGSKVLKTEIVGTKNVYIYAHCAKKSNGIT 433

Query: 361 LLFINLSNATDFIIDLENNINLSSGKRNASKGQRVKIDSSREEYHLTPKDGLVRSSTVLL 420
           +L +N    +   I L+ +                K  S REEYHLTP +  ++S  V L
Sbjct: 434 MLVLNHDGESSVKISLDPS----------------KYGSKREEYHLTPVNNNLQSRLVKL 493

Query: 421 NGNPLETTKEGDIPNLIPVYRQSNSSIHIAGWSIAFIVVP 447
           NG  L     G IP L PV + ++  + +A +S  F+ +P
Sbjct: 494 NGELLHLDPSGVIPALNPVEKDNSKQLEVAPYSFMFVHLP 513

BLAST of CmaCh07G012140 vs. ExPASy Swiss-Prot
Match: Q90YK5 (Heparanase OS=Gallus gallus OX=9031 GN=HPSE PE=1 SV=1)

HSP 1 Score: 153.7 bits (387), Expect = 5.1e-36
Identity = 118/403 (29.28%), Postives = 191/403 (47.39%), Query Frame = 0

Query: 65  DDLNNFFNNTGAIVTFGLNALLGKYKTQGIQWEGNWNYSNAEALIQYTVENNYQINSWEF 124
           D L+ F +++G  + FGLNALL   +  G+QW+     SNA+ L+ Y  + +Y I SWE 
Sbjct: 150 DILHTFASSSGFRLVFGLNALL---RRAGLQWDS----SNAKQLLGYCAQRSYNI-SWEL 209

Query: 125 GNELAGI-NSIGASVTAAQYAKDVIKLREIIDR--LYNNSQQKPMIVAPGAFFDEKWYAE 184
           GNE        G  +   Q  +D + LR+++ +  LY +++   + V       +     
Sbjct: 210 GNEPNSFRKKSGICIDGFQLGRDFVHLRQLLSQHPLYRHAELYGLDVGQPRKHTQHLLRS 269

Query: 185 FLRKTGPGVVDVLTHHIYNMGAGDDPKLVYRFVNSTYLSQVSNTFEHLESVIQKNAPWGA 244
           F++  G  +  V  HH Y  G     +    F++   L   +     +  +++   P   
Sbjct: 270 FMKSGGKAIDSVTWHHYYVNGRSATRE---DFLSPEVLDSFATAIHDVLGIVEATVPGKK 329

Query: 245 AWVGEAGGAYQGGSPHISYTFINSFWYLDQLGMASMYNTKVYCRQTLIG-GFYGVIKSST 304
            W+GE G AY GG+P +S T++  F +LD+LG+A+     V  RQ   G G Y ++ +  
Sbjct: 330 VWLGETGSAYGGGAPQLSNTYVAGFMWLDKLGLAARRGIDVVMRQVSFGAGSYHLVDAG- 389

Query: 305 LLPTPDYYGALLFHRLMGPRVLK--IDNNVSSDLRSYAHCSRGR------SGVTLLFINL 364
             P PDY+ +LL+ RL+G RVL+  ++   +   R Y HC+  R        VTL  +NL
Sbjct: 390 FKPLPDYWLSLLYKRLVGTRVLQASVEQADARRPRVYLHCTNPRHPKYREGDVTLFALNL 449

Query: 365 SNATDFIIDLENNINLSSGKRNASKGQRVKIDSSREEYHLTP--KDGLVRSSTVLLNGNP 424
           SN T          +L   K+  SK        S ++Y L P  KD ++ S  V LNG  
Sbjct: 450 SNVTQ---------SLQLPKQLWSK--------SVDQYLLLPHGKDSIL-SREVQLNGRL 509

Query: 425 LETTKEGDIPNLIPVYRQSNSSIHIAGWSIAFIVVPHFVAPAC 454
           L+   +  +P L  +     S++ +  +S  F V+ +  A AC
Sbjct: 510 LQMVDDETLPALHEMALAPGSTLGLPAFSYGFYVIRNAKAIAC 522

BLAST of CmaCh07G012140 vs. ExPASy TrEMBL
Match: A0A6J1HRV1 (heparanase-like protein 1 OS=Cucurbita maxima OX=3661 GN=LOC111466171 PE=3 SV=1)

HSP 1 Score: 937.2 bits (2421), Expect = 2.6e-269
Identity = 454/454 (100.00%), Postives = 454/454 (100.00%), Query Frame = 0

Query: 1   DLTLPIMNKAVQAFKSVRIRVGGTLQDKLIYNVGAGFQGTCHPFQATKGSLFDFSAGCLY 60
           DLTLPIMNKAVQAFKSVRIRVGGTLQDKLIYNVGAGFQGTCHPFQATKGSLFDFSAGCLY
Sbjct: 50  DLTLPIMNKAVQAFKSVRIRVGGTLQDKLIYNVGAGFQGTCHPFQATKGSLFDFSAGCLY 109

Query: 61  MERWDDLNNFFNNTGAIVTFGLNALLGKYKTQGIQWEGNWNYSNAEALIQYTVENNYQIN 120
           MERWDDLNNFFNNTGAIVTFGLNALLGKYKTQGIQWEGNWNYSNAEALIQYTVENNYQIN
Sbjct: 110 MERWDDLNNFFNNTGAIVTFGLNALLGKYKTQGIQWEGNWNYSNAEALIQYTVENNYQIN 169

Query: 121 SWEFGNELAGINSIGASVTAAQYAKDVIKLREIIDRLYNNSQQKPMIVAPGAFFDEKWYA 180
           SWEFGNELAGINSIGASVTAAQYAKDVIKLREIIDRLYNNSQQKPMIVAPGAFFDEKWYA
Sbjct: 170 SWEFGNELAGINSIGASVTAAQYAKDVIKLREIIDRLYNNSQQKPMIVAPGAFFDEKWYA 229

Query: 181 EFLRKTGPGVVDVLTHHIYNMGAGDDPKLVYRFVNSTYLSQVSNTFEHLESVIQKNAPWG 240
           EFLRKTGPGVVDVLTHHIYNMGAGDDPKLVYRFVNSTYLSQVSNTFEHLESVIQKNAPWG
Sbjct: 230 EFLRKTGPGVVDVLTHHIYNMGAGDDPKLVYRFVNSTYLSQVSNTFEHLESVIQKNAPWG 289

Query: 241 AAWVGEAGGAYQGGSPHISYTFINSFWYLDQLGMASMYNTKVYCRQTLIGGFYGVIKSST 300
           AAWVGEAGGAYQGGSPHISYTFINSFWYLDQLGMASMYNTKVYCRQTLIGGFYGVIKSST
Sbjct: 290 AAWVGEAGGAYQGGSPHISYTFINSFWYLDQLGMASMYNTKVYCRQTLIGGFYGVIKSST 349

Query: 301 LLPTPDYYGALLFHRLMGPRVLKIDNNVSSDLRSYAHCSRGRSGVTLLFINLSNATDFII 360
           LLPTPDYYGALLFHRLMGPRVLKIDNNVSSDLRSYAHCSRGRSGVTLLFINLSNATDFII
Sbjct: 350 LLPTPDYYGALLFHRLMGPRVLKIDNNVSSDLRSYAHCSRGRSGVTLLFINLSNATDFII 409

Query: 361 DLENNINLSSGKRNASKGQRVKIDSSREEYHLTPKDGLVRSSTVLLNGNPLETTKEGDIP 420
           DLENNINLSSGKRNASKGQRVKIDSSREEYHLTPKDGLVRSSTVLLNGNPLETTKEGDIP
Sbjct: 410 DLENNINLSSGKRNASKGQRVKIDSSREEYHLTPKDGLVRSSTVLLNGNPLETTKEGDIP 469

Query: 421 NLIPVYRQSNSSIHIAGWSIAFIVVPHFVAPACN 455
           NLIPVYRQSNSSIHIAGWSIAFIVVPHFVAPACN
Sbjct: 470 NLIPVYRQSNSSIHIAGWSIAFIVVPHFVAPACN 503

BLAST of CmaCh07G012140 vs. ExPASy TrEMBL
Match: A0A6J1HU12 (heparanase-like protein 1 OS=Cucurbita maxima OX=3661 GN=LOC111466170 PE=3 SV=1)

HSP 1 Score: 928.7 bits (2399), Expect = 9.3e-267
Identity = 449/454 (98.90%), Postives = 451/454 (99.34%), Query Frame = 0

Query: 1   DLTLPIMNKAVQAFKSVRIRVGGTLQDKLIYNVGAGFQGTCHPFQATKGSLFDFSAGCLY 60
           DLTLPI+NKAVQAFKSVRIRVGGTLQDKLIYNVGAGFQGTCHPFQATKGSLFDFS GCLY
Sbjct: 73  DLTLPIINKAVQAFKSVRIRVGGTLQDKLIYNVGAGFQGTCHPFQATKGSLFDFSVGCLY 132

Query: 61  MERWDDLNNFFNNTGAIVTFGLNALLGKYKTQGIQWEGNWNYSNAEALIQYTVENNYQIN 120
           MERWDDLNNFFNNTGAIVTFGLNALLGKYKTQGIQWEGNWNYSNAEALIQYTVENNYQIN
Sbjct: 133 MERWDDLNNFFNNTGAIVTFGLNALLGKYKTQGIQWEGNWNYSNAEALIQYTVENNYQIN 192

Query: 121 SWEFGNELAGINSIGASVTAAQYAKDVIKLREIIDRLYNNSQQKPMIVAPGAFFDEKWYA 180
           SWEFGNELAGINSIGASVTAAQYAKDVIKLREIIDRLYNNSQQKPMIVAPGAFFDEKWYA
Sbjct: 193 SWEFGNELAGINSIGASVTAAQYAKDVIKLREIIDRLYNNSQQKPMIVAPGAFFDEKWYA 252

Query: 181 EFLRKTGPGVVDVLTHHIYNMGAGDDPKLVYRFVNSTYLSQVSNTFEHLESVIQKNAPWG 240
           EFLRKTGPGVVDVLTHHIYNMGAGDDPKL+YRFVNSTYLSQVSNTFE LESVIQKNAPWG
Sbjct: 253 EFLRKTGPGVVDVLTHHIYNMGAGDDPKLIYRFVNSTYLSQVSNTFERLESVIQKNAPWG 312

Query: 241 AAWVGEAGGAYQGGSPHISYTFINSFWYLDQLGMASMYNTKVYCRQTLIGGFYGVIKSST 300
           AAWVGEAGGAYQGGSPHISYTFINSFWYLDQLGMASMYNTKVYCRQTLIGGFYGVIKSST
Sbjct: 313 AAWVGEAGGAYQGGSPHISYTFINSFWYLDQLGMASMYNTKVYCRQTLIGGFYGVIKSST 372

Query: 301 LLPTPDYYGALLFHRLMGPRVLKIDNNVSSDLRSYAHCSRGRSGVTLLFINLSNATDFII 360
           LLPTPDYYGALLFHRLMGPRVLKIDNNVSSDLRSYAHCSRGRSGVTLLFINLSNATDFII
Sbjct: 373 LLPTPDYYGALLFHRLMGPRVLKIDNNVSSDLRSYAHCSRGRSGVTLLFINLSNATDFII 432

Query: 361 DLENNINLSSGKRNASKGQRVKIDSSREEYHLTPKDGLVRSSTVLLNGNPLETTKEGDIP 420
           DLENNINLSSGKRNASKGQRVKIDS REEYHLTPKDGLVRSSTVLLNGNPLETTKEGDIP
Sbjct: 433 DLENNINLSSGKRNASKGQRVKIDSPREEYHLTPKDGLVRSSTVLLNGNPLETTKEGDIP 492

Query: 421 NLIPVYRQSNSSIHIAGWSIAFIVVPHFVAPACN 455
           NLIPVYRQSNSSIHIAGWSIAFIVVPHFVAPACN
Sbjct: 493 NLIPVYRQSNSSIHIAGWSIAFIVVPHFVAPACN 526

BLAST of CmaCh07G012140 vs. ExPASy TrEMBL
Match: A0A6J1EAY5 (heparanase-like protein 1 OS=Cucurbita moschata OX=3662 GN=LOC111432361 PE=3 SV=1)

HSP 1 Score: 916.8 bits (2368), Expect = 3.6e-263
Identity = 438/454 (96.48%), Postives = 448/454 (98.68%), Query Frame = 0

Query: 1   DLTLPIMNKAVQAFKSVRIRVGGTLQDKLIYNVGAGFQGTCHPFQATKGSLFDFSAGCLY 60
           DLTLPI+NKAVQAFKSVRIRVGGTLQDKLIYNVGAGF+GTCHPFQATKGSLFDFS GCLY
Sbjct: 73  DLTLPIINKAVQAFKSVRIRVGGTLQDKLIYNVGAGFKGTCHPFQATKGSLFDFSVGCLY 132

Query: 61  MERWDDLNNFFNNTGAIVTFGLNALLGKYKTQGIQWEGNWNYSNAEALIQYTVENNYQIN 120
           MERWDDLNNFFNNTGAIVTFGLNALLGKYKTQGIQWEGNWNYSN EALIQYT+ENNYQIN
Sbjct: 133 MERWDDLNNFFNNTGAIVTFGLNALLGKYKTQGIQWEGNWNYSNTEALIQYTIENNYQIN 192

Query: 121 SWEFGNELAGINSIGASVTAAQYAKDVIKLREIIDRLYNNSQQKPMIVAPGAFFDEKWYA 180
           SWEFGNELAG+NSIGASVTAAQYAKDVIKLREIIDRLYNN QQKPMIVAPGAFFDEKWY 
Sbjct: 193 SWEFGNELAGLNSIGASVTAAQYAKDVIKLREIIDRLYNNFQQKPMIVAPGAFFDEKWYG 252

Query: 181 EFLRKTGPGVVDVLTHHIYNMGAGDDPKLVYRFVNSTYLSQVSNTFEHLESVIQKNAPWG 240
           EFLRKTGPGVVDVLTHHIYNMGAGDDPKL+YRFVNSTYLSQVSNTFEHLE+VIQKNAPWG
Sbjct: 253 EFLRKTGPGVVDVLTHHIYNMGAGDDPKLIYRFVNSTYLSQVSNTFEHLENVIQKNAPWG 312

Query: 241 AAWVGEAGGAYQGGSPHISYTFINSFWYLDQLGMASMYNTKVYCRQTLIGGFYGVIKSST 300
           AAWVGEAGGAYQGGSPHISYTFINSFWYLDQLGMASMYNTKVYCRQTLIGGFYGVIKSST
Sbjct: 313 AAWVGEAGGAYQGGSPHISYTFINSFWYLDQLGMASMYNTKVYCRQTLIGGFYGVIKSST 372

Query: 301 LLPTPDYYGALLFHRLMGPRVLKIDNNVSSDLRSYAHCSRGRSGVTLLFINLSNATDFII 360
           LLPTPDYYGALLFHRLMGPRVLKIDNNVSSDLRSYAHCSRGR+GVTLLFINLSN TDFII
Sbjct: 373 LLPTPDYYGALLFHRLMGPRVLKIDNNVSSDLRSYAHCSRGRTGVTLLFINLSNTTDFII 432

Query: 361 DLENNINLSSGKRNASKGQRVKIDSSREEYHLTPKDGLVRSSTVLLNGNPLETTKEGDIP 420
           D+ENNINLSSGKRNASKGQR+KIDS REEYHLTPKDGLVRSSTVLLNGNPLETTKEGDIP
Sbjct: 433 DVENNINLSSGKRNASKGQRMKIDSPREEYHLTPKDGLVRSSTVLLNGNPLETTKEGDIP 492

Query: 421 NLIPVYRQSNSSIHIAGWSIAFIVVPHFVAPACN 455
           NL+PVYRQSNSSIHIAGWSIAFIVVPHFVAPACN
Sbjct: 493 NLLPVYRQSNSSIHIAGWSIAFIVVPHFVAPACN 526

BLAST of CmaCh07G012140 vs. ExPASy TrEMBL
Match: A0A6J1I2E3 (heparanase-like protein 1 OS=Cucurbita maxima OX=3661 GN=LOC111470271 PE=3 SV=1)

HSP 1 Score: 813.1 bits (2099), Expect = 5.7e-232
Identity = 390/458 (85.15%), Postives = 421/458 (91.92%), Query Frame = 0

Query: 1   DLTLPIMNKAVQAFKSVRIRVGGTLQDKLIYNVGAGFQGTCHPFQATKGSLFDFSAGCLY 60
           DLTLPI+NKAVQAFKSVRIRVGGTLQDKLIYNVG GF+GTCHPFQAT GSLFDFSAGCLY
Sbjct: 80  DLTLPILNKAVQAFKSVRIRVGGTLQDKLIYNVGTGFKGTCHPFQATVGSLFDFSAGCLY 139

Query: 61  MERWDDLNNFFNNTGAIVTFGLNALLGKYKTQGIQWEGNWNYSNAEALIQYTVENNYQIN 120
           MERWDDLNNFFNNTGAIVTFGLNALLGK+ T+GIQWEG WNYSNAEALIQYTVE NY+IN
Sbjct: 140 MERWDDLNNFFNNTGAIVTFGLNALLGKHNTKGIQWEGKWNYSNAEALIQYTVEKNYRIN 199

Query: 121 SWEFGNELAGINSIGASVTAAQYAKDVIKLREIIDRLYNNSQQKPMIVAPGAFFDEKWYA 180
           SWEFGNELAG  SIGAS+TAAQYA+D++KLREIIDRLYNNSQQKP+IVAPGAFFDE WY 
Sbjct: 200 SWEFGNELAGRLSIGASITAAQYAEDLLKLREIIDRLYNNSQQKPLIVAPGAFFDEAWYD 259

Query: 181 EFLRKTGPGVVDVLTHHIYNMGAGDDPKLVYRFVNSTYLSQVSNTFEHLESVIQKNAPWG 240
           EF+RKTGP VVDVLTHHIYNMGAG+DPKL+YRFVNSTYLSQVSNTFE LE+VIQKNAPW 
Sbjct: 260 EFVRKTGPKVVDVLTHHIYNMGAGNDPKLIYRFVNSTYLSQVSNTFEQLENVIQKNAPWA 319

Query: 241 AAWVGEAGGAYQGGSPHISYTFINSFWYLDQLGMASMYNTKVYCRQTLIGGFYGVIKSST 300
           AAWVGEAGGAYQGGSPH+S TFINSFWYLDQLGMAS+YNTKVYCRQTLIGGFYGV+KSST
Sbjct: 320 AAWVGEAGGAYQGGSPHLSDTFINSFWYLDQLGMASLYNTKVYCRQTLIGGFYGVLKSST 379

Query: 301 LLPTPDYYGALLFHRLMGPRVLKIDNNVSSDLRSYAHCSRGRSGVTLLFINLSNATDFII 360
           LLPTPDYYGALLFHRLMGP VLKI+N VSSDLRSYAHCSRGRSGVTLL INLSN T+F+I
Sbjct: 380 LLPTPDYYGALLFHRLMGPGVLKINNKVSSDLRSYAHCSRGRSGVTLLLINLSNTTNFLI 439

Query: 361 DLENNINLSSGKRN-----ASKGQRVKIDSSREEYHLTPKDGLVRSSTVLLNGNPLETTK 420
            ++N++N+S  K N     +    R K  +SREEYHLTPKDGL+RSSTVLLNGNPLETTK
Sbjct: 440 TVQNDMNMSLRKSNTRTNGSHSKYRQKRTASREEYHLTPKDGLLRSSTVLLNGNPLETTK 499

Query: 421 EGDIPNLIPVYRQSNSSIHIAGWSIAFIVVPHFVAPAC 454
           EGDIPNL+PVYRQSNS IHIA WSIAF+V+P FV PAC
Sbjct: 500 EGDIPNLVPVYRQSNSYIHIASWSIAFVVIPDFVTPAC 537

BLAST of CmaCh07G012140 vs. ExPASy TrEMBL
Match: A0A6J1G385 (heparanase-like protein 1 OS=Cucurbita moschata OX=3662 GN=LOC111450381 PE=3 SV=1)

HSP 1 Score: 807.0 bits (2083), Expect = 4.1e-230
Identity = 387/453 (85.43%), Postives = 414/453 (91.39%), Query Frame = 0

Query: 1   DLTLPIMNKAVQAFKSVRIRVGGTLQDKLIYNVGAGFQGTCHPFQATKGSLFDFSAGCLY 60
           DLTLPI+NKAVQAFKSVRIRVGGTLQDKLIYNVGAGF+GTCHPFQA  GSLFDFS GCLY
Sbjct: 50  DLTLPILNKAVQAFKSVRIRVGGTLQDKLIYNVGAGFKGTCHPFQAIVGSLFDFSVGCLY 109

Query: 61  MERWDDLNNFFNNTGAIVTFGLNALLGKYKTQGIQWEGNWNYSNAEALIQYTVENNYQIN 120
           MERWDDLNNFFNNTGAIVTFGLNALLGK+ T+GIQWEG WNYSNAEALIQYTVE NY+IN
Sbjct: 110 MERWDDLNNFFNNTGAIVTFGLNALLGKHNTKGIQWEGKWNYSNAEALIQYTVEKNYKIN 169

Query: 121 SWEFGNELAGINSIGASVTAAQYAKDVIKLREIIDRLYNNSQQKPMIVAPGAFFDEKWYA 180
           SWEFGNEL G  SIGAS+TAAQYA+D++KLREIIDRLYNNSQQKP+IVAPGAFFDE WY 
Sbjct: 170 SWEFGNELVGRISIGASITAAQYAEDLLKLREIIDRLYNNSQQKPLIVAPGAFFDEAWYD 229

Query: 181 EFLRKTGPGVVDVLTHHIYNMGAGDDPKLVYRFVNSTYLSQVSNTFEHLESVIQKNAPWG 240
           EF+RKTGP  VDVLTHHIYNMGAG DPKL+YRFVNSTYLSQVSNTFE LE+VIQKNAPW 
Sbjct: 230 EFVRKTGPKAVDVLTHHIYNMGAGHDPKLIYRFVNSTYLSQVSNTFEQLENVIQKNAPWA 289

Query: 241 AAWVGEAGGAYQGGSPHISYTFINSFWYLDQLGMASMYNTKVYCRQTLIGGFYGVIKSST 300
           AAWVGEAGGAYQGGSPH+S TFINSFWYLDQLGMAS+YNTKVYCRQTLIGGFYGV+KSST
Sbjct: 290 AAWVGEAGGAYQGGSPHLSDTFINSFWYLDQLGMASLYNTKVYCRQTLIGGFYGVLKSST 349

Query: 301 LLPTPDYYGALLFHRLMGPRVLKIDNNVSSDLRSYAHCSRGRSGVTLLFINLSNATDFII 360
           LLPTPDYYGALLFHRLMGP VLKIDN VSSDLRSYAHCSRGRSGVTLL INLSN T+F+I
Sbjct: 350 LLPTPDYYGALLFHRLMGPGVLKIDNKVSSDLRSYAHCSRGRSGVTLLLINLSNTTNFLI 409

Query: 361 DLENNINLSSGKRNASKGQRVKIDSSREEYHLTPKDGLVRSSTVLLNGNPLETTKEGDIP 420
            ++N++N+S  KR A           REEYHLTPKDGL+RSSTVLLNGNPLETTKEGDIP
Sbjct: 410 TIQNDMNMSLQKRTA----------LREEYHLTPKDGLLRSSTVLLNGNPLETTKEGDIP 469

Query: 421 NLIPVYRQSNSSIHIAGWSIAFIVVPHFVAPAC 454
           NL+PVYRQSNS IHIA WSIAF+V+P FVAPAC
Sbjct: 470 NLVPVYRQSNSYIHIASWSIAFVVLPDFVAPAC 492

BLAST of CmaCh07G012140 vs. NCBI nr
Match: XP_022966520.1 (heparanase-like protein 1 [Cucurbita maxima])

HSP 1 Score: 937.2 bits (2421), Expect = 5.4e-269
Identity = 454/454 (100.00%), Postives = 454/454 (100.00%), Query Frame = 0

Query: 1   DLTLPIMNKAVQAFKSVRIRVGGTLQDKLIYNVGAGFQGTCHPFQATKGSLFDFSAGCLY 60
           DLTLPIMNKAVQAFKSVRIRVGGTLQDKLIYNVGAGFQGTCHPFQATKGSLFDFSAGCLY
Sbjct: 50  DLTLPIMNKAVQAFKSVRIRVGGTLQDKLIYNVGAGFQGTCHPFQATKGSLFDFSAGCLY 109

Query: 61  MERWDDLNNFFNNTGAIVTFGLNALLGKYKTQGIQWEGNWNYSNAEALIQYTVENNYQIN 120
           MERWDDLNNFFNNTGAIVTFGLNALLGKYKTQGIQWEGNWNYSNAEALIQYTVENNYQIN
Sbjct: 110 MERWDDLNNFFNNTGAIVTFGLNALLGKYKTQGIQWEGNWNYSNAEALIQYTVENNYQIN 169

Query: 121 SWEFGNELAGINSIGASVTAAQYAKDVIKLREIIDRLYNNSQQKPMIVAPGAFFDEKWYA 180
           SWEFGNELAGINSIGASVTAAQYAKDVIKLREIIDRLYNNSQQKPMIVAPGAFFDEKWYA
Sbjct: 170 SWEFGNELAGINSIGASVTAAQYAKDVIKLREIIDRLYNNSQQKPMIVAPGAFFDEKWYA 229

Query: 181 EFLRKTGPGVVDVLTHHIYNMGAGDDPKLVYRFVNSTYLSQVSNTFEHLESVIQKNAPWG 240
           EFLRKTGPGVVDVLTHHIYNMGAGDDPKLVYRFVNSTYLSQVSNTFEHLESVIQKNAPWG
Sbjct: 230 EFLRKTGPGVVDVLTHHIYNMGAGDDPKLVYRFVNSTYLSQVSNTFEHLESVIQKNAPWG 289

Query: 241 AAWVGEAGGAYQGGSPHISYTFINSFWYLDQLGMASMYNTKVYCRQTLIGGFYGVIKSST 300
           AAWVGEAGGAYQGGSPHISYTFINSFWYLDQLGMASMYNTKVYCRQTLIGGFYGVIKSST
Sbjct: 290 AAWVGEAGGAYQGGSPHISYTFINSFWYLDQLGMASMYNTKVYCRQTLIGGFYGVIKSST 349

Query: 301 LLPTPDYYGALLFHRLMGPRVLKIDNNVSSDLRSYAHCSRGRSGVTLLFINLSNATDFII 360
           LLPTPDYYGALLFHRLMGPRVLKIDNNVSSDLRSYAHCSRGRSGVTLLFINLSNATDFII
Sbjct: 350 LLPTPDYYGALLFHRLMGPRVLKIDNNVSSDLRSYAHCSRGRSGVTLLFINLSNATDFII 409

Query: 361 DLENNINLSSGKRNASKGQRVKIDSSREEYHLTPKDGLVRSSTVLLNGNPLETTKEGDIP 420
           DLENNINLSSGKRNASKGQRVKIDSSREEYHLTPKDGLVRSSTVLLNGNPLETTKEGDIP
Sbjct: 410 DLENNINLSSGKRNASKGQRVKIDSSREEYHLTPKDGLVRSSTVLLNGNPLETTKEGDIP 469

Query: 421 NLIPVYRQSNSSIHIAGWSIAFIVVPHFVAPACN 455
           NLIPVYRQSNSSIHIAGWSIAFIVVPHFVAPACN
Sbjct: 470 NLIPVYRQSNSSIHIAGWSIAFIVVPHFVAPACN 503

BLAST of CmaCh07G012140 vs. NCBI nr
Match: XP_022966519.1 (heparanase-like protein 1 [Cucurbita maxima])

HSP 1 Score: 928.7 bits (2399), Expect = 1.9e-266
Identity = 449/454 (98.90%), Postives = 451/454 (99.34%), Query Frame = 0

Query: 1   DLTLPIMNKAVQAFKSVRIRVGGTLQDKLIYNVGAGFQGTCHPFQATKGSLFDFSAGCLY 60
           DLTLPI+NKAVQAFKSVRIRVGGTLQDKLIYNVGAGFQGTCHPFQATKGSLFDFS GCLY
Sbjct: 73  DLTLPIINKAVQAFKSVRIRVGGTLQDKLIYNVGAGFQGTCHPFQATKGSLFDFSVGCLY 132

Query: 61  MERWDDLNNFFNNTGAIVTFGLNALLGKYKTQGIQWEGNWNYSNAEALIQYTVENNYQIN 120
           MERWDDLNNFFNNTGAIVTFGLNALLGKYKTQGIQWEGNWNYSNAEALIQYTVENNYQIN
Sbjct: 133 MERWDDLNNFFNNTGAIVTFGLNALLGKYKTQGIQWEGNWNYSNAEALIQYTVENNYQIN 192

Query: 121 SWEFGNELAGINSIGASVTAAQYAKDVIKLREIIDRLYNNSQQKPMIVAPGAFFDEKWYA 180
           SWEFGNELAGINSIGASVTAAQYAKDVIKLREIIDRLYNNSQQKPMIVAPGAFFDEKWYA
Sbjct: 193 SWEFGNELAGINSIGASVTAAQYAKDVIKLREIIDRLYNNSQQKPMIVAPGAFFDEKWYA 252

Query: 181 EFLRKTGPGVVDVLTHHIYNMGAGDDPKLVYRFVNSTYLSQVSNTFEHLESVIQKNAPWG 240
           EFLRKTGPGVVDVLTHHIYNMGAGDDPKL+YRFVNSTYLSQVSNTFE LESVIQKNAPWG
Sbjct: 253 EFLRKTGPGVVDVLTHHIYNMGAGDDPKLIYRFVNSTYLSQVSNTFERLESVIQKNAPWG 312

Query: 241 AAWVGEAGGAYQGGSPHISYTFINSFWYLDQLGMASMYNTKVYCRQTLIGGFYGVIKSST 300
           AAWVGEAGGAYQGGSPHISYTFINSFWYLDQLGMASMYNTKVYCRQTLIGGFYGVIKSST
Sbjct: 313 AAWVGEAGGAYQGGSPHISYTFINSFWYLDQLGMASMYNTKVYCRQTLIGGFYGVIKSST 372

Query: 301 LLPTPDYYGALLFHRLMGPRVLKIDNNVSSDLRSYAHCSRGRSGVTLLFINLSNATDFII 360
           LLPTPDYYGALLFHRLMGPRVLKIDNNVSSDLRSYAHCSRGRSGVTLLFINLSNATDFII
Sbjct: 373 LLPTPDYYGALLFHRLMGPRVLKIDNNVSSDLRSYAHCSRGRSGVTLLFINLSNATDFII 432

Query: 361 DLENNINLSSGKRNASKGQRVKIDSSREEYHLTPKDGLVRSSTVLLNGNPLETTKEGDIP 420
           DLENNINLSSGKRNASKGQRVKIDS REEYHLTPKDGLVRSSTVLLNGNPLETTKEGDIP
Sbjct: 433 DLENNINLSSGKRNASKGQRVKIDSPREEYHLTPKDGLVRSSTVLLNGNPLETTKEGDIP 492

Query: 421 NLIPVYRQSNSSIHIAGWSIAFIVVPHFVAPACN 455
           NLIPVYRQSNSSIHIAGWSIAFIVVPHFVAPACN
Sbjct: 493 NLIPVYRQSNSSIHIAGWSIAFIVVPHFVAPACN 526

BLAST of CmaCh07G012140 vs. NCBI nr
Match: KAG6595639.1 (Heparanase-like protein 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 919.1 bits (2374), Expect = 1.5e-263
Identity = 440/454 (96.92%), Postives = 450/454 (99.12%), Query Frame = 0

Query: 1   DLTLPIMNKAVQAFKSVRIRVGGTLQDKLIYNVGAGFQGTCHPFQATKGSLFDFSAGCLY 60
           DLTLPI+NKAVQAFKSVRIRVGGTLQDKLIYNVGAGF+GTCHPFQATKGSLFDFS GCLY
Sbjct: 73  DLTLPIINKAVQAFKSVRIRVGGTLQDKLIYNVGAGFEGTCHPFQATKGSLFDFSVGCLY 132

Query: 61  MERWDDLNNFFNNTGAIVTFGLNALLGKYKTQGIQWEGNWNYSNAEALIQYTVENNYQIN 120
           MERWDDLNNFFNNTGAIVTFGLNALLGKYKTQGIQWEGNWNYSNAEALIQYTVENNYQIN
Sbjct: 133 MERWDDLNNFFNNTGAIVTFGLNALLGKYKTQGIQWEGNWNYSNAEALIQYTVENNYQIN 192

Query: 121 SWEFGNELAGINSIGASVTAAQYAKDVIKLREIIDRLYNNSQQKPMIVAPGAFFDEKWYA 180
           SWEFGNELAG+NSIGASVTAAQYAKDVIKLREIIDRLY+NSQQKPMIVAPGAFFDEKWY 
Sbjct: 193 SWEFGNELAGLNSIGASVTAAQYAKDVIKLREIIDRLYSNSQQKPMIVAPGAFFDEKWYG 252

Query: 181 EFLRKTGPGVVDVLTHHIYNMGAGDDPKLVYRFVNSTYLSQVSNTFEHLESVIQKNAPWG 240
           EFLRKTGPGVVDVLTHHIYNMGAGDDPKL+YRFVNSTYLSQVSNTFEHLE+VIQKNAPWG
Sbjct: 253 EFLRKTGPGVVDVLTHHIYNMGAGDDPKLIYRFVNSTYLSQVSNTFEHLENVIQKNAPWG 312

Query: 241 AAWVGEAGGAYQGGSPHISYTFINSFWYLDQLGMASMYNTKVYCRQTLIGGFYGVIKSST 300
           AAWVGEAGGAYQGGSPHISYTFINSFWYLDQLGMASMYNTKVYCRQTLIGGFYGVIKSST
Sbjct: 313 AAWVGEAGGAYQGGSPHISYTFINSFWYLDQLGMASMYNTKVYCRQTLIGGFYGVIKSST 372

Query: 301 LLPTPDYYGALLFHRLMGPRVLKIDNNVSSDLRSYAHCSRGRSGVTLLFINLSNATDFII 360
           LLPTPDYYGALLFHRLMGPRVLKIDNNVSSDLRSYAHCSRGR+GVTLLFINLSN TDFII
Sbjct: 373 LLPTPDYYGALLFHRLMGPRVLKIDNNVSSDLRSYAHCSRGRTGVTLLFINLSNTTDFII 432

Query: 361 DLENNINLSSGKRNASKGQRVKIDSSREEYHLTPKDGLVRSSTVLLNGNPLETTKEGDIP 420
           D+ENNINLSSGKRNASKGQR+KIDS REEYHLTPKDGLVRSSTVLLNGNPLETTKEGDIP
Sbjct: 433 DVENNINLSSGKRNASKGQRMKIDSLREEYHLTPKDGLVRSSTVLLNGNPLETTKEGDIP 492

Query: 421 NLIPVYRQSNSSIHIAGWSIAFIVVPHFVAPACN 455
           NL+PVYRQSNSSIHIAGWSIAFIVVPHFVAPACN
Sbjct: 493 NLLPVYRQSNSSIHIAGWSIAFIVVPHFVAPACN 526

BLAST of CmaCh07G012140 vs. NCBI nr
Match: XP_022924976.1 (heparanase-like protein 1 [Cucurbita moschata])

HSP 1 Score: 916.8 bits (2368), Expect = 7.5e-263
Identity = 438/454 (96.48%), Postives = 448/454 (98.68%), Query Frame = 0

Query: 1   DLTLPIMNKAVQAFKSVRIRVGGTLQDKLIYNVGAGFQGTCHPFQATKGSLFDFSAGCLY 60
           DLTLPI+NKAVQAFKSVRIRVGGTLQDKLIYNVGAGF+GTCHPFQATKGSLFDFS GCLY
Sbjct: 73  DLTLPIINKAVQAFKSVRIRVGGTLQDKLIYNVGAGFKGTCHPFQATKGSLFDFSVGCLY 132

Query: 61  MERWDDLNNFFNNTGAIVTFGLNALLGKYKTQGIQWEGNWNYSNAEALIQYTVENNYQIN 120
           MERWDDLNNFFNNTGAIVTFGLNALLGKYKTQGIQWEGNWNYSN EALIQYT+ENNYQIN
Sbjct: 133 MERWDDLNNFFNNTGAIVTFGLNALLGKYKTQGIQWEGNWNYSNTEALIQYTIENNYQIN 192

Query: 121 SWEFGNELAGINSIGASVTAAQYAKDVIKLREIIDRLYNNSQQKPMIVAPGAFFDEKWYA 180
           SWEFGNELAG+NSIGASVTAAQYAKDVIKLREIIDRLYNN QQKPMIVAPGAFFDEKWY 
Sbjct: 193 SWEFGNELAGLNSIGASVTAAQYAKDVIKLREIIDRLYNNFQQKPMIVAPGAFFDEKWYG 252

Query: 181 EFLRKTGPGVVDVLTHHIYNMGAGDDPKLVYRFVNSTYLSQVSNTFEHLESVIQKNAPWG 240
           EFLRKTGPGVVDVLTHHIYNMGAGDDPKL+YRFVNSTYLSQVSNTFEHLE+VIQKNAPWG
Sbjct: 253 EFLRKTGPGVVDVLTHHIYNMGAGDDPKLIYRFVNSTYLSQVSNTFEHLENVIQKNAPWG 312

Query: 241 AAWVGEAGGAYQGGSPHISYTFINSFWYLDQLGMASMYNTKVYCRQTLIGGFYGVIKSST 300
           AAWVGEAGGAYQGGSPHISYTFINSFWYLDQLGMASMYNTKVYCRQTLIGGFYGVIKSST
Sbjct: 313 AAWVGEAGGAYQGGSPHISYTFINSFWYLDQLGMASMYNTKVYCRQTLIGGFYGVIKSST 372

Query: 301 LLPTPDYYGALLFHRLMGPRVLKIDNNVSSDLRSYAHCSRGRSGVTLLFINLSNATDFII 360
           LLPTPDYYGALLFHRLMGPRVLKIDNNVSSDLRSYAHCSRGR+GVTLLFINLSN TDFII
Sbjct: 373 LLPTPDYYGALLFHRLMGPRVLKIDNNVSSDLRSYAHCSRGRTGVTLLFINLSNTTDFII 432

Query: 361 DLENNINLSSGKRNASKGQRVKIDSSREEYHLTPKDGLVRSSTVLLNGNPLETTKEGDIP 420
           D+ENNINLSSGKRNASKGQR+KIDS REEYHLTPKDGLVRSSTVLLNGNPLETTKEGDIP
Sbjct: 433 DVENNINLSSGKRNASKGQRMKIDSPREEYHLTPKDGLVRSSTVLLNGNPLETTKEGDIP 492

Query: 421 NLIPVYRQSNSSIHIAGWSIAFIVVPHFVAPACN 455
           NL+PVYRQSNSSIHIAGWSIAFIVVPHFVAPACN
Sbjct: 493 NLLPVYRQSNSSIHIAGWSIAFIVVPHFVAPACN 526

BLAST of CmaCh07G012140 vs. NCBI nr
Match: KAG6595546.1 (Heparanase-like protein 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 905.2 bits (2338), Expect = 2.3e-259
Identity = 433/454 (95.37%), Postives = 446/454 (98.24%), Query Frame = 0

Query: 1   DLTLPIMNKAVQAFKSVRIRVGGTLQDKLIYNVGAGFQGTCHPFQATKGSLFDFSAGCLY 60
           DLTLPI+NKAVQAFKS+RIRVGGTLQDKLIYNVGAGF+GTCHPFQATKGSLFDFS GCLY
Sbjct: 73  DLTLPIINKAVQAFKSLRIRVGGTLQDKLIYNVGAGFEGTCHPFQATKGSLFDFSVGCLY 132

Query: 61  MERWDDLNNFFNNTGAIVTFGLNALLGKYKTQGIQWEGNWNYSNAEALIQYTVENNYQIN 120
           MERWDDLNNFFNNTGAIVTFGLNALLGKYKTQGIQWEGNWNYSNAEALIQYTVENNYQIN
Sbjct: 133 MERWDDLNNFFNNTGAIVTFGLNALLGKYKTQGIQWEGNWNYSNAEALIQYTVENNYQIN 192

Query: 121 SWEFGNELAGINSIGASVTAAQYAKDVIKLREIIDRLYNNSQQKPMIVAPGAFFDEKWYA 180
           SWEFGNELAG+NSIGASVTAAQYAKDVIKLREIIDRLYNNSQQKPMIVAPGAFFDEKWY 
Sbjct: 193 SWEFGNELAGLNSIGASVTAAQYAKDVIKLREIIDRLYNNSQQKPMIVAPGAFFDEKWYE 252

Query: 181 EFLRKTGPGVVDVLTHHIYNMGAGDDPKLVYRFVNSTYLSQVSNTFEHLESVIQKNAPWG 240
           EFLRKTGPGVVDVLTHHIYNMGAGDDPKL+YRFVNSTYLSQVS+TFEHLE+VI+KNAPWG
Sbjct: 253 EFLRKTGPGVVDVLTHHIYNMGAGDDPKLIYRFVNSTYLSQVSSTFEHLENVIRKNAPWG 312

Query: 241 AAWVGEAGGAYQGGSPHISYTFINSFWYLDQLGMASMYNTKVYCRQTLIGGFYGVIKSST 300
           AAWVGEAGGAYQGGSPHISYTFINSFWYLDQLGMASMYNTKVYCRQTLIGGFYGVIKSST
Sbjct: 313 AAWVGEAGGAYQGGSPHISYTFINSFWYLDQLGMASMYNTKVYCRQTLIGGFYGVIKSST 372

Query: 301 LLPTPDYYGALLFHRLMGPRVLKIDNNVSSDLRSYAHCSRGRSGVTLLFINLSNATDFII 360
           LLPTPDYYGALLFHRLMGPRVLKIDNNVSSDLRSYAHCSRGRSGVTLLFINLSNATDFII
Sbjct: 373 LLPTPDYYGALLFHRLMGPRVLKIDNNVSSDLRSYAHCSRGRSGVTLLFINLSNATDFII 432

Query: 361 DLENNINLSSGKRNASKGQRVKIDSSREEYHLTPKDGLVRSSTVLLNGNPLETTKEGDIP 420
           ++ENNINLS  K  AS GQR+KIDS REEYHLTPKDGLVRSSTVLLNGNPLETTKEGDIP
Sbjct: 433 NVENNINLSLRKGKASNGQRIKIDSPREEYHLTPKDGLVRSSTVLLNGNPLETTKEGDIP 492

Query: 421 NLIPVYRQSNSSIHIAGWSIAFIVVPHFVAPACN 455
           NL+PVYRQSNSSIHIAGWSIAFIV+PHFVAPACN
Sbjct: 493 NLLPVYRQSNSSIHIAGWSIAFIVIPHFVAPACN 526

BLAST of CmaCh07G012140 vs. TAIR 10
Match: AT5G07830.1 (glucuronidase 2 )

HSP 1 Score: 517.3 bits (1331), Expect = 1.2e-146
Identity = 243/470 (51.70%), Postives = 339/470 (72.13%), Query Frame = 0

Query: 1   DLTLPIMNKAVQAFKSVRIRVGGTLQDKLIYNVGAGFQGTCHPFQATKGSLFDFSAGCLY 60
           DLT P++ KA++AFK +RIR+GG+LQD++IY+VG   +  C PFQ     LF FS GCL+
Sbjct: 76  DLTRPLLTKAIKAFKPLRIRIGGSLQDQVIYDVG-NLKTPCRPFQKMNSGLFGFSKGCLH 135

Query: 61  MERWDDLNNFFNNTGAIVTFGLNALLGKYKTQGIQWEGNWNYSNAEALIQYTVENNYQIN 120
           M+RWD+LN+F   TGA+VTFGLNAL G++K +G  W G W++ N +  + YTV   Y I+
Sbjct: 136 MKRWDELNSFLTATGAVVTFGLNALRGRHKLRGKAWGGAWDHINTQDFLNYTVSKGYVID 195

Query: 121 SWEFGNELAGINSIGASVTAAQYAKDVIKLREIIDRLYNNS-QQKPMIVAPGAFFDEKWY 180
           SWEFGNEL+G + +GASV+A  Y KD+I L+++I+++Y NS   KP++VAPG F++++WY
Sbjct: 196 SWEFGNELSG-SGVGASVSAELYGKDLIVLKDVINKVYKNSWLHKPILVAPGGFYEQQWY 255

Query: 181 AEFLRKTGPGVVDVLTHHIYNMGAGDDPKLVYRFVNSTYLSQVSNTFEHLESVIQKNAPW 240
            + L  +GP VVDV+THHIYN+G+G+DP LV + ++ +YLSQVS TF+ +   IQ++ PW
Sbjct: 256 TKLLEISGPSVVDVVTHHIYNLGSGNDPALVKKIMDPSYLSQVSKTFKDVNQTIQEHGPW 315

Query: 241 GAAWVGEAGGAYQGGSPHISYTFINSFWYLDQLGMASMYNTKVYCRQTLIGGFYGVIKSS 300
            + WVGE+GGAY  G  H+S TFI+SFWYLDQLGM++ +NTKVYCRQTL+GGFYG+++  
Sbjct: 316 ASPWVGESGGAYNSGGRHVSDTFIDSFWYLDQLGMSARHNTKVYCRQTLVGGFYGLLEKG 375

Query: 301 TLLPTPDYYGALLFHRLMGPRVLKIDNNVSSDLRSYAHCSRGRSGVTLLFINLSNATDFI 360
           T +P PDYY ALL+HRLMG  VL +  +    LR YAHCS+GR+GVTLL INLSN +DF 
Sbjct: 376 TFVPNPDYYSALLWHRLMGKGVLAVQTDGPPQLRVYAHCSKGRAGVTLLLINLSNQSDFT 435

Query: 361 IDLENNINL---SSGKRNASKGQRVKIDSS------------REEYHLTPKDGLVRSSTV 420
           + + N IN+   +  ++  S    +K   S            REEYHLTP++G++RS T+
Sbjct: 436 VSVSNGINVVLNAESRKKKSLLDTLKRPFSWIGSKASDGYLNREEYHLTPENGVLRSKTM 495

Query: 421 LLNGNPLETTKEGDIPNLIPVYRQSNSSIHIAGWSIAFIVVPHFVAPACN 455
           +LNG  L+ T  GDIP+L PV R  NS +++   S++FIV+P+F A AC+
Sbjct: 496 VLNGKSLKPTATGDIPSLEPVLRSVNSPLNVLPLSMSFIVLPNFDASACS 543

BLAST of CmaCh07G012140 vs. TAIR 10
Match: AT5G61250.2 (glucuronidase 1 )

HSP 1 Score: 508.8 bits (1309), Expect = 4.4e-144
Identity = 246/469 (52.45%), Postives = 329/469 (70.15%), Query Frame = 0

Query: 1   DLTLPIMNKAVQAFKSVRIRVGGTLQDKLIYNVGAGFQGTCHPFQATKGSLFDFSAGCLY 60
           +L  P++ KA+QAF+++RIR+GG+LQD++IY+VG   +  C  F+ T   LF FS GCLY
Sbjct: 73  NLASPLLAKAIQAFRTLRIRIGGSLQDQVIYDVG-DLKTPCTQFKKTDDGLFGFSEGCLY 132

Query: 61  MERWDDLNNFFNNTGAIVTFGLNALLGKYKTQGIQWEGNWNYSNAEALIQYTVENNYQIN 120
           M+RWD++N+FFN TGAIVTFGLNAL G+ K  G  W G+W+++N +  + YTV   Y I+
Sbjct: 133 MKRWDEVNHFFNATGAIVTFGLNALHGRNKLNGTAWGGDWDHTNTQDFMNYTVSKGYAID 192

Query: 121 SWEFGNELAGINSIGASVTAAQYAKDVIKLREIIDRLYNNSQQKPMIVAPGAFFDEKWYA 180
           SWEFGNEL+G + I ASV+   Y KD+I L+ +I  +Y NS+ KP++VAPG FF+E+WY+
Sbjct: 193 SWEFGNELSG-SGIWASVSVELYGKDLIVLKNVIKNVYKNSRTKPLVVAPGGFFEEQWYS 252

Query: 181 EFLRKTGPGVVDVLTHHIYNMGAGDDPKLVYRFVNSTYLSQVSNTFEHLESVIQKNAPWG 240
           E LR +GPGV+DVLTHHIYN+G G+DPKLV + ++  YLS +S  F ++   IQ++ PW 
Sbjct: 253 ELLRLSGPGVLDVLTHHIYNLGPGNDPKLVNKILDPNYLSGISELFANVNQTIQEHGPWA 312

Query: 241 AAWVGEAGGAYQGGSPHISYTFINSFWYLDQLGMASMYNTKVYCRQTLIGGFYGVIKSST 300
           AAWVGEAGGA+  G   +S TFINSFWYLDQLG++S +NTKVYCRQ L+GGFYG+++  T
Sbjct: 313 AAWVGEAGGAFNSGGRQVSETFINSFWYLDQLGISSKHNTKVYCRQALVGGFYGLLEKET 372

Query: 301 LLPTPDYYGALLFHRLMGPRVLKIDNNVSSDLRSYAHCSRGRSGVTLLFINLSNATDFII 360
            +P PDYY ALL+HRLMG  +L +    S  LR+Y HCS+ R+G+T+L INLS  T F +
Sbjct: 373 FVPNPDYYSALLWHRLMGKGILGVQTTASEYLRAYVHCSKRRAGITILLINLSKHTTFTV 432

Query: 361 DLENNINL----SSGKRNA------SKGQRVKIDSS-----REEYHLTPKDGLVRSSTVL 420
            + N + +     S KR +      SK   V   +S     REEYHL+PKDG +RS  +L
Sbjct: 433 AVSNGVKVVLQAESMKRKSFLETIKSKVSWVGNKASDGYLNREEYHLSPKDGDLRSKIML 492

Query: 421 LNGNPLETTKEGDIPNLIPVYRQSNSSIHIAGWSIAFIVVPHFVAPACN 455
           LNG PL  T  GDIP L PV     S ++I   SI+FIV+P F APAC+
Sbjct: 493 LNGKPLVPTATGDIPKLEPVRHGVKSPVYINPLSISFIVLPTFDAPACS 539

BLAST of CmaCh07G012140 vs. TAIR 10
Match: AT5G61250.1 (glucuronidase 1 )

HSP 1 Score: 508.8 bits (1309), Expect = 4.4e-144
Identity = 246/469 (52.45%), Postives = 329/469 (70.15%), Query Frame = 0

Query: 1   DLTLPIMNKAVQAFKSVRIRVGGTLQDKLIYNVGAGFQGTCHPFQATKGSLFDFSAGCLY 60
           +L  P++ KA+QAF+++RIR+GG+LQD++IY+VG   +  C  F+ T   LF FS GCLY
Sbjct: 73  NLASPLLAKAIQAFRTLRIRIGGSLQDQVIYDVG-DLKTPCTQFKKTDDGLFGFSEGCLY 132

Query: 61  MERWDDLNNFFNNTGAIVTFGLNALLGKYKTQGIQWEGNWNYSNAEALIQYTVENNYQIN 120
           M+RWD++N+FFN TGAIVTFGLNAL G+ K  G  W G+W+++N +  + YTV   Y I+
Sbjct: 133 MKRWDEVNHFFNATGAIVTFGLNALHGRNKLNGTAWGGDWDHTNTQDFMNYTVSKGYAID 192

Query: 121 SWEFGNELAGINSIGASVTAAQYAKDVIKLREIIDRLYNNSQQKPMIVAPGAFFDEKWYA 180
           SWEFGNEL+G + I ASV+   Y KD+I L+ +I  +Y NS+ KP++VAPG FF+E+WY+
Sbjct: 193 SWEFGNELSG-SGIWASVSVELYGKDLIVLKNVIKNVYKNSRTKPLVVAPGGFFEEQWYS 252

Query: 181 EFLRKTGPGVVDVLTHHIYNMGAGDDPKLVYRFVNSTYLSQVSNTFEHLESVIQKNAPWG 240
           E LR +GPGV+DVLTHHIYN+G G+DPKLV + ++  YLS +S  F ++   IQ++ PW 
Sbjct: 253 ELLRLSGPGVLDVLTHHIYNLGPGNDPKLVNKILDPNYLSGISELFANVNQTIQEHGPWA 312

Query: 241 AAWVGEAGGAYQGGSPHISYTFINSFWYLDQLGMASMYNTKVYCRQTLIGGFYGVIKSST 300
           AAWVGEAGGA+  G   +S TFINSFWYLDQLG++S +NTKVYCRQ L+GGFYG+++  T
Sbjct: 313 AAWVGEAGGAFNSGGRQVSETFINSFWYLDQLGISSKHNTKVYCRQALVGGFYGLLEKET 372

Query: 301 LLPTPDYYGALLFHRLMGPRVLKIDNNVSSDLRSYAHCSRGRSGVTLLFINLSNATDFII 360
            +P PDYY ALL+HRLMG  +L +    S  LR+Y HCS+ R+G+T+L INLS  T F +
Sbjct: 373 FVPNPDYYSALLWHRLMGKGILGVQTTASEYLRAYVHCSKRRAGITILLINLSKHTTFTV 432

Query: 361 DLENNINL----SSGKRNA------SKGQRVKIDSS-----REEYHLTPKDGLVRSSTVL 420
            + N + +     S KR +      SK   V   +S     REEYHL+PKDG +RS  +L
Sbjct: 433 AVSNGVKVVLQAESMKRKSFLETIKSKVSWVGNKASDGYLNREEYHLSPKDGDLRSKIML 492

Query: 421 LNGNPLETTKEGDIPNLIPVYRQSNSSIHIAGWSIAFIVVPHFVAPACN 455
           LNG PL  T  GDIP L PV     S ++I   SI+FIV+P F APAC+
Sbjct: 493 LNGKPLVPTATGDIPKLEPVRHGVKSPVYINPLSISFIVLPTFDAPACS 539

BLAST of CmaCh07G012140 vs. TAIR 10
Match: AT5G34940.2 (glucuronidase 3 )

HSP 1 Score: 405.2 bits (1040), Expect = 6.9e-113
Identity = 206/464 (44.40%), Postives = 289/464 (62.28%), Query Frame = 0

Query: 1   DLTLPIMNKAVQAFKSVRIRVGGTLQDKLIYNVGAGFQGTCHPFQATKGSLFDFSAGCLY 60
           DL   I+  A++AF  ++IR+GGTLQD +IY      Q  C PF      LF ++ GCL 
Sbjct: 77  DLNNVILQNAIKAFAPLKIRIGGTLQDIVIYETPDSKQ-PCLPFTKNSSILFGYTQGCLP 136

Query: 61  MERWDDLNNFFNNTGAIVTFGLNALLGKYKTQGIQWEGNWNYSNAEALIQYTVENNYQIN 120
           M RWD+LN FF  TG  V FGLNAL G+      +  G WNY+NAE+ I++T ENNY I+
Sbjct: 137 MRRWDELNAFFRKTGTKVIFGLNALSGRSIKSNGEAIGAWNYTNAESFIRFTAENNYTID 196

Query: 121 SWEFGNELAGINSIGASVTAAQYAKDVIKLREIIDRLYNNSQQKPMIVAPGAFFDEKWYA 180
            WE GNEL G + +GA V A QYA D I LR I++R+Y N    P+++ PG FF+  W+ 
Sbjct: 197 GWELGNELCG-SGVGARVGANQYAIDTINLRNIVNRVYKNVSPMPLVIGPGGFFEVDWFT 256

Query: 181 EFLRKTGPGVVDVLTHHIYNMGAGDDPKLVYRFVNSTYLSQVSNTFEHLESVIQKNAPWG 240
           E+L K     ++  T HIY++G G D  L+ + +N +YL Q + +F  L+++I+ ++   
Sbjct: 257 EYLNK-AENSLNATTRHIYDLGPGVDEHLIEKILNPSYLDQEAKSFRSLKNIIKNSSTKA 316

Query: 241 AAWVGEAGGAYQGGSPHISYTFINSFWYLDQLGMASMYNTKVYCRQTLIGGFYGVIKSST 300
            AWVGE+GGAY  G   +S  F+ SFWYLDQLGMAS+Y+TK YCRQ+LIGG YG++ ++ 
Sbjct: 317 VAWVGESGGAYNSGRNLVSNAFVYSFWYLDQLGMASLYDTKTYCRQSLIGGNYGLLNTTN 376

Query: 301 LLPTPDYYGALLFHRLMGPRVLKIDNNVSSDLRSYAHCSRGRSGVTLLFINLSNATDFI- 360
             P PDYY AL++ +LMG + L    + +  +RSY HC+R   G+T+L +NL N T  + 
Sbjct: 377 FTPNPDYYSALIWRQLMGRKALFTTFSGTKKIRSYTHCARQSKGITVLLMNLDNTTTVVA 436

Query: 361 -IDLENNINL------SSGKRNASK---GQRVKIDSSREEYHLTPKDGLVRSSTVLLNGN 420
            ++L N+ +L       S KR +S+   G    I   REEYHLT KDG + S T+LLNGN
Sbjct: 437 KVELNNSFSLRHTKHMKSYKRASSQLFGGPNGVI--QREEYHLTAKDGNLHSQTMLLNGN 496

Query: 421 PLETTKEGDIPNLIPVYRQSNSSIHIAGWSIAFIVVPHFVAPAC 454
            L+    GD+P + P++  S   I IA +SI F+ + + V PAC
Sbjct: 497 ALQVNSMGDLPPIEPIHINSTEPITIAPYSIVFVHMRNVVVPAC 535

BLAST of CmaCh07G012140 vs. TAIR 10
Match: AT5G34940.1 (glucuronidase 3 )

HSP 1 Score: 359.8 bits (922), Expect = 3.3e-99
Identity = 181/404 (44.80%), Postives = 255/404 (63.12%), Query Frame = 0

Query: 61  MERWDDLNNFFNNTGAIVTFGLNALLGKYKTQGIQWEGNWNYSNAEALIQYTVENNYQIN 120
           M RWD+LN FF  TG  V FGLNAL G+      +  G WNY+NAE+ I++T ENNY I+
Sbjct: 1   MRRWDELNAFFRKTGTKVIFGLNALSGRSIKSNGEAIGAWNYTNAESFIRFTAENNYTID 60

Query: 121 SWEFGNELAGINSIGASVTAAQYAKDVIKLREIIDRLYNNSQQKPMIVAPGAFFDEKWYA 180
            WE GNEL G + +GA V A QYA D I LR I++R+Y N    P+++ PG FF+  W+ 
Sbjct: 61  GWELGNELCG-SGVGARVGANQYAIDTINLRNIVNRVYKNVSPMPLVIGPGGFFEVDWFT 120

Query: 181 EFLRKTGPGVVDVLTHHIYNMGAGDDPKLVYRFVNSTYLSQVSNTFEHLESVIQKNAPWG 240
           E+L K     ++  T HIY++G G D  L+ + +N +YL Q + +F  L+++I+ ++   
Sbjct: 121 EYLNK-AENSLNATTRHIYDLGPGVDEHLIEKILNPSYLDQEAKSFRSLKNIIKNSSTKA 180

Query: 241 AAWVGEAGGAYQGGSPHISYTFINSFWYLDQLGMASMYNTKVYCRQTLIGGFYGVIKSST 300
            AWVGE+GGAY  G   +S  F+ SFWYLDQLGMAS+Y+TK YCRQ+LIGG YG++ ++ 
Sbjct: 181 VAWVGESGGAYNSGRNLVSNAFVYSFWYLDQLGMASLYDTKTYCRQSLIGGNYGLLNTTN 240

Query: 301 LLPTPDYYGALLFHRLMGPRVLKIDNNVSSDLRSYAHCSRGRSGVTLLFINLSNATDFI- 360
             P PDYY AL++ +LMG + L    + +  +RSY HC+R   G+T+L +NL N T  + 
Sbjct: 241 FTPNPDYYSALIWRQLMGRKALFTTFSGTKKIRSYTHCARQSKGITVLLMNLDNTTTVVA 300

Query: 361 -IDLENNINL------SSGKRNASK---GQRVKIDSSREEYHLTPKDGLVRSSTVLLNGN 420
            ++L N+ +L       S KR +S+   G    I   REEYHLT KDG + S T+LLNGN
Sbjct: 301 KVELNNSFSLRHTKHMKSYKRASSQLFGGPNGVI--QREEYHLTAKDGNLHSQTMLLNGN 360

Query: 421 PLETTKEGDIPNLIPVYRQSNSSIHIAGWSIAFIVVPHFVAPAC 454
            L+    GD+P + P++  S   I IA +SI F+ + + V PAC
Sbjct: 361 ALQVNSMGDLPPIEPIHINSTEPITIAPYSIVFVHMRNVVVPAC 400

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9FF101.7e-14551.70Heparanase-like protein 1 OS=Arabidopsis thaliana OX=3702 GN=At5g07830 PE=2 SV=1[more]
Q8L6086.2e-14352.45Heparanase-like protein 2 OS=Arabidopsis thaliana OX=3702 GN=At5g61250 PE=2 SV=1[more]
Q9FZP19.6e-11244.40Heparanase-like protein 3 OS=Arabidopsis thaliana OX=3702 GN=At5g34940 PE=2 SV=2[more]
Q9LRC83.9e-8937.17Baicalin-beta-D-glucuronidase OS=Scutellaria baicalensis OX=65409 GN=SGUS PE=1 S... [more]
Q90YK55.1e-3629.28Heparanase OS=Gallus gallus OX=9031 GN=HPSE PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1HRV12.6e-269100.00heparanase-like protein 1 OS=Cucurbita maxima OX=3661 GN=LOC111466171 PE=3 SV=1[more]
A0A6J1HU129.3e-26798.90heparanase-like protein 1 OS=Cucurbita maxima OX=3661 GN=LOC111466170 PE=3 SV=1[more]
A0A6J1EAY53.6e-26396.48heparanase-like protein 1 OS=Cucurbita moschata OX=3662 GN=LOC111432361 PE=3 SV=... [more]
A0A6J1I2E35.7e-23285.15heparanase-like protein 1 OS=Cucurbita maxima OX=3661 GN=LOC111470271 PE=3 SV=1[more]
A0A6J1G3854.1e-23085.43heparanase-like protein 1 OS=Cucurbita moschata OX=3662 GN=LOC111450381 PE=3 SV=... [more]
Match NameE-valueIdentityDescription
XP_022966520.15.4e-269100.00heparanase-like protein 1 [Cucurbita maxima][more]
XP_022966519.11.9e-26698.90heparanase-like protein 1 [Cucurbita maxima][more]
KAG6595639.11.5e-26396.92Heparanase-like protein 1, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022924976.17.5e-26396.48heparanase-like protein 1 [Cucurbita moschata][more]
KAG6595546.12.3e-25995.37Heparanase-like protein 1, partial [Cucurbita argyrosperma subsp. sororia][more]
Match NameE-valueIdentityDescription
AT5G07830.11.2e-14651.70glucuronidase 2 [more]
AT5G61250.24.4e-14452.45glucuronidase 1 [more]
AT5G61250.14.4e-14452.45glucuronidase 1 [more]
AT5G34940.26.9e-11344.40glucuronidase 3 [more]
AT5G34940.13.3e-9944.80glucuronidase 3 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.20.20.80Glycosidasescoord: 2..315
e-value: 7.6E-64
score: 218.1
NoneNo IPR availablePANTHERPTHR14363:SF21HEPARANASE-LIKE PROTEIN 1coord: 1..454
NoneNo IPR availablePANTHERPTHR14363HEPARANASE-RELATEDcoord: 1..454
IPR005199Glycoside hydrolase, family 79PFAMPF03662Glyco_hydro_79ncoord: 1..273
e-value: 6.4E-116
score: 386.7
IPR017853Glycoside hydrolase superfamilySUPERFAMILY51445(Trans)glycosidasescoord: 9..317

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh07G012140.1CmaCh07G012140.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016020 membrane
molecular_function GO:0004566 beta-glucuronidase activity
molecular_function GO:0016798 hydrolase activity, acting on glycosyl bonds