CmaCh07G007010 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh07G007010
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Descriptionmitogen-activated protein kinase kinase kinase 18-like
LocationCma_Chr07: 3081457 .. 3082521 (-)
RNA-Seq ExpressionCmaCh07G007010
SyntenyCmaCh07G007010
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAGTGGATTCGAGGGCATGAGATTGGGTGTGGGAGTTTTGCGACCATCAATTTAGCCATGCCTTCTAACACTTCTAATGCTCAGTTTCCTCCGTTAATGGCCGTTAAATCGTCTCCAGCTGTCTCCTCTGCCTCGCTAAAGAACGAGAAGCGGGTTTTGGATCTTTTGGGTGATTGCCCTCAAATTATACGGTGTTTTGGTGAGAGCTGCAGCGTCGAGAGAGGCGACGAGTTATATAATTTGTTTCTTGAGTATGCTAGTGGGGGTAGTTTGGCCGACAGAATACAGAGCCACGGTGGTTGGTTGCCGGAATTTGAGGTTCGGCGGTTCACGAGGACGATTCTCGAAGGGCTTCGGTATATTCACGAGAAAGGATTTGTTCACTGCGACGTGAAGCCTCGGAATATTCTAGTGTTTGGTGACGGCGATGCTAAAATTGCTGATTTTGGATTGTCGAAGAAGGCGGGGAAAAACAGAGCAGAAACAGGGGAAGAGGGGAAATTTCAATTGAGAGGCTCTCCTCTGTACATGTCGCCGGAATCGGTGAACGATGACGAGTACGAGTCGCCGTGTGACATTTGGGCTGTCGGATGCGCGGTGGTGGAGATGTTGACCGGAAAACCCGCTTGGAATTTCCCAGTGGAATGCAACATCTATTCTCTGTTACTGAAAATTGGGATCGGAGAGGAATTGCCGAAAATCCCAAAATATATTTCCGAGGAAGGGAAAGATTTTCTGGGTAAATGCCTGGTGAAGGATCCCGCAAAGAGATGGACGGCTGAGATGCTTCTGAACCATCCATTTCTAGCAGACTCCGGCAGCAGCTCTGTTCCATTGACGCCCAAGGGCGAACCATTAACGTCGCCGAGATGCCCTTTCGATTTCGAAGATTGGGCATCAATTCACTCGCTAGAGATGGATCAGAAATTTGAGGAAGAATCAAATTGTTGGTTGGATTCGTCGTGTCCGTGGTCGTGTTCACCGACGGAGCGGCTGCTTCAGCTAGTGGACAACGGGCCAGTTGACTGGGTCATCACTGACAGTTGGGTCACCGTCAGATGA

mRNA sequence

ATGGAGTGGATTCGAGGGCATGAGATTGGGTGTGGGAGTTTTGCGACCATCAATTTAGCCATGCCTTCTAACACTTCTAATGCTCAGTTTCCTCCGTTAATGGCCGTTAAATCGTCTCCAGCTGTCTCCTCTGCCTCGCTAAAGAACGAGAAGCGGGTTTTGGATCTTTTGGGTGATTGCCCTCAAATTATACGGTGTTTTGGTGAGAGCTGCAGCGTCGAGAGAGGCGACGAGTTATATAATTTGTTTCTTGAGTATGCTAGTGGGGGTAGTTTGGCCGACAGAATACAGAGCCACGGTGGTTGGTTGCCGGAATTTGAGGTTCGGCGGTTCACGAGGACGATTCTCGAAGGGCTTCGGTATATTCACGAGAAAGGATTTGTTCACTGCGACGTGAAGCCTCGGAATATTCTAGTGTTTGGTGACGGCGATGCTAAAATTGCTGATTTTGGATTGTCGAAGAAGGCGGGGAAAAACAGAGCAGAAACAGGGGAAGAGGGGAAATTTCAATTGAGAGGCTCTCCTCTGTACATGTCGCCGGAATCGGTGAACGATGACGAGTACGAGTCGCCGTGTGACATTTGGGCTGTCGGATGCGCGGTGGTGGAGATGTTGACCGGAAAACCCGCTTGGAATTTCCCAGTGGAATGCAACATCTATTCTCTGTTACTGAAAATTGGGATCGGAGAGGAATTGCCGAAAATCCCAAAATATATTTCCGAGGAAGGGAAAGATTTTCTGGGTAAATGCCTGGTGAAGGATCCCGCAAAGAGATGGACGGCTGAGATGCTTCTGAACCATCCATTTCTAGCAGACTCCGGCAGCAGCTCTGTTCCATTGACGCCCAAGGGCGAACCATTAACGTCGCCGAGATGCCCTTTCGATTTCGAAGATTGGGCATCAATTCACTCGCTAGAGATGGATCAGAAATTTGAGGAAGAATCAAATTGTTGGTTGGATTCGTCGTGTCCGTGGTCGTGTTCACCGACGGAGCGGCTGCTTCAGCTAGTGGACAACGGGCCAGTTGACTGGGTCATCACTGACAGTTGGGTCACCGTCAGATGA

Coding sequence (CDS)

ATGGAGTGGATTCGAGGGCATGAGATTGGGTGTGGGAGTTTTGCGACCATCAATTTAGCCATGCCTTCTAACACTTCTAATGCTCAGTTTCCTCCGTTAATGGCCGTTAAATCGTCTCCAGCTGTCTCCTCTGCCTCGCTAAAGAACGAGAAGCGGGTTTTGGATCTTTTGGGTGATTGCCCTCAAATTATACGGTGTTTTGGTGAGAGCTGCAGCGTCGAGAGAGGCGACGAGTTATATAATTTGTTTCTTGAGTATGCTAGTGGGGGTAGTTTGGCCGACAGAATACAGAGCCACGGTGGTTGGTTGCCGGAATTTGAGGTTCGGCGGTTCACGAGGACGATTCTCGAAGGGCTTCGGTATATTCACGAGAAAGGATTTGTTCACTGCGACGTGAAGCCTCGGAATATTCTAGTGTTTGGTGACGGCGATGCTAAAATTGCTGATTTTGGATTGTCGAAGAAGGCGGGGAAAAACAGAGCAGAAACAGGGGAAGAGGGGAAATTTCAATTGAGAGGCTCTCCTCTGTACATGTCGCCGGAATCGGTGAACGATGACGAGTACGAGTCGCCGTGTGACATTTGGGCTGTCGGATGCGCGGTGGTGGAGATGTTGACCGGAAAACCCGCTTGGAATTTCCCAGTGGAATGCAACATCTATTCTCTGTTACTGAAAATTGGGATCGGAGAGGAATTGCCGAAAATCCCAAAATATATTTCCGAGGAAGGGAAAGATTTTCTGGGTAAATGCCTGGTGAAGGATCCCGCAAAGAGATGGACGGCTGAGATGCTTCTGAACCATCCATTTCTAGCAGACTCCGGCAGCAGCTCTGTTCCATTGACGCCCAAGGGCGAACCATTAACGTCGCCGAGATGCCCTTTCGATTTCGAAGATTGGGCATCAATTCACTCGCTAGAGATGGATCAGAAATTTGAGGAAGAATCAAATTGTTGGTTGGATTCGTCGTGTCCGTGGTCGTGTTCACCGACGGAGCGGCTGCTTCAGCTAGTGGACAACGGGCCAGTTGACTGGGTCATCACTGACAGTTGGGTCACCGTCAGATGA

Protein sequence

MEWIRGHEIGCGSFATINLAMPSNTSNAQFPPLMAVKSSPAVSSASLKNEKRVLDLLGDCPQIIRCFGESCSVERGDELYNLFLEYASGGSLADRIQSHGGWLPEFEVRRFTRTILEGLRYIHEKGFVHCDVKPRNILVFGDGDAKIADFGLSKKAGKNRAETGEEGKFQLRGSPLYMSPESVNDDEYESPCDIWAVGCAVVEMLTGKPAWNFPVECNIYSLLLKIGIGEELPKIPKYISEEGKDFLGKCLVKDPAKRWTAEMLLNHPFLADSGSSSVPLTPKGEPLTSPRCPFDFEDWASIHSLEMDQKFEEESNCWLDSSCPWSCSPTERLLQLVDNGPVDWVITDSWVTVR
Homology
BLAST of CmaCh07G007010 vs. ExPASy Swiss-Prot
Match: Q9SND6 (Mitogen-activated protein kinase kinase kinase 20 OS=Arabidopsis thaliana OX=3702 GN=MAPKKK20 PE=1 SV=1)

HSP 1 Score: 367.9 bits (943), Expect = 1.3e-100
Identity = 191/361 (52.91%), Postives = 239/361 (66.20%), Query Frame = 0

Query: 1   MEWIRGHEIGCGSFATINLAMPSNTSNAQFPPLMAVKSSPAVSSASLKNEKRVLDLLGDC 60
           MEW+RG  IG G+F+T++ A  S  S   FP L+AVKS+ A  +ASL NEK VLD LGDC
Sbjct: 1   MEWVRGETIGFGTFSTVSTATKSRNS-GDFPALIAVKSTDAYGAASLSNEKSVLDSLGDC 60

Query: 61  PQIIRCFGESCSVERGDELYNLFLEYASGGSLADRIQSHGG-WLPEFEVRRFTRTILEGL 120
           P+IIRC+GE  +VE G+E++NL LEYAS GSLA  ++  GG  LPE  VRR T ++L GL
Sbjct: 61  PEIIRCYGEDSTVENGEEMHNLLLEYASRGSLASYMKKLGGEGLPESTVRRHTGSVLRGL 120

Query: 121 RYIHEKGFVHCDVKPRNILVFGDGDAKIADFGLSKKAGKNRAETGEEGKFQLRGSPLYMS 180
           R+IH KGF HCD+K  NIL+F DG  KIADFGL+ +   +   T      ++RG+PLYM+
Sbjct: 121 RHIHAKGFAHCDIKLANILLFNDGSVKIADFGLAMRVDGDL--TALRKSVEIRGTPLYMA 180

Query: 181 PESVNDDEYESPCDIWAVGCAVVEMLTGKPAWNFPVECNIYSLLLKIGIGEELPKIPKYI 240
           PE VND+EY S  D+WA+GCAVVEM +GK AW+     +  SLL++IG+G+ELPKIP+ +
Sbjct: 181 PECVNDNEYGSAADVWALGCAVVEMFSGKTAWSVKEGSHFMSLLIRIGVGDELPKIPEML 240

Query: 241 SEEGKDFLGKCLVKDPAKRWTAEMLLNHPF----LADSGSSSVPLTPKGEP--LTSPRCP 300
           SEEGKDFL KC VKDPAKRWTAEMLLNH F    L D    +  +  K E   L SP+CP
Sbjct: 241 SEEGKDFLSKCFVKDPAKRWTAEMLLNHSFVTIDLEDDHRENFVVKVKDEDKVLMSPKCP 300

Query: 301 FDFEDWASIHSLEMDQKFEEESNCWLDSSCPWSCSPTERLLQLVDNGPVDWVITDSWVTV 355
           F+F+DW S         F  +SN   D       SP ERL  LV     DW +  SW+TV
Sbjct: 301 FEFDDWDS---------FTLDSNPSFD-------SPVERLGSLVSGSIPDWSVGGSWLTV 342

BLAST of CmaCh07G007010 vs. ExPASy Swiss-Prot
Match: O80888 (Mitogen-activated protein kinase kinase kinase 17 OS=Arabidopsis thaliana OX=3702 GN=MAPKKK17 PE=1 SV=1)

HSP 1 Score: 219.9 bits (559), Expect = 4.5e-56
Identity = 134/361 (37.12%), Postives = 188/361 (52.08%), Query Frame = 0

Query: 1   MEWIRGHEIGCGSFATINLAMPSNTSNAQFPPLMAVKSSPAVSSASLKNEKRVLDLLGDC 60
           MEW RG  +G GS AT+  A   N+       ++AVKSS    S  L+ E ++L  L   
Sbjct: 1   MEWTRGRILGRGSTATVYAAAGHNSDE-----ILAVKSSEVHRSEFLQREAKILSSLSS- 60

Query: 61  PQIIRCFGESCSVE-RGDELYNLFLEYASGGSLADRIQSHGGWLPEFEVRRFTRTILEGL 120
           P +I   G     E  G  +YNL +EYA  G+L D     GG + E  V ++TR IL+GL
Sbjct: 61  PYVIGYRGSETKRESNGVVMYNLLMEYAPYGTLTDAAAKDGGRVDETRVVKYTRDILKGL 120

Query: 121 RYIHEKGFVHCDVKPRNILVFGDGDAKIADFGLSKKAGKNRAETGEEGKFQLRGSPLYMS 180
            YIH KG VHCDVK  N+++   G+AKIADFG +K+            +  + G+P +M+
Sbjct: 121 EYIHSKGIVHCDVKGSNVVISEKGEAKIADFGCAKR-------VDPVFESPVMGTPAFMA 180

Query: 181 PESVNDDEYESPCDIWAVGCAVVEMLTGKPAW-NFPVECNIYSLLLKIGIGEELPKIPKY 240
           PE    ++     DIWAVGC ++EM+TG P W       +  S+L ++G   E P++P  
Sbjct: 181 PEVARGEKQGKESDIWAVGCTMIEMVTGSPPWTKADSREDPVSVLYRVGYSSETPELPCL 240

Query: 241 ISEEGKDFLGKCLVKDPAKRWTAEMLLNHPFLADSGSSSVPLTPKGEPLTSPRCPFDFED 300
           ++EE KDFL KCL ++  +RWTA  LLNHPFL         L P G    SP    D   
Sbjct: 241 LAEEAKDFLEKCLKREANERWTATQLLNHPFLTTKPDIEPVLVP-GLISNSPTSVTDQTF 300

Query: 301 WASIHSLEMDQKFE-EESNCWLDSSCPWSCSPTERLLQLVDNGPVDWVITD----SWVTV 355
           W S+   E ++  E ++ +  LD    W C  +ER+ +L   G +D    D     W+ V
Sbjct: 301 WRSVEEEEEEETEEIQKDSRDLDRLSLWGCY-SERIGRLKCVGGLDGTRCDMEGGDWIMV 346

BLAST of CmaCh07G007010 vs. ExPASy Swiss-Prot
Match: Q9ZVP5 (Mitogen-activated protein kinase kinase kinase 18 OS=Arabidopsis thaliana OX=3702 GN=MAPKKK18 PE=1 SV=1)

HSP 1 Score: 209.5 bits (532), Expect = 6.1e-53
Identity = 133/367 (36.24%), Postives = 189/367 (51.50%), Query Frame = 0

Query: 1   MEWIRGHEIGCGSFATINLAMPSNTSNAQFPPLMAVKSSPAVSSASLKNEKRVLDLLGDC 60
           M W RG  +G GS AT++ A    +        +AVKS+    S  L+ E ++L  L + 
Sbjct: 1   MNWTRGKTLGRGSTATVSAATCHESGET-----LAVKSAEFHRSEFLQREAKILSSL-NS 60

Query: 61  PQIIRCFGESCSVER------GD-ELYNLFLEYASGGSLADRIQSHGGWLPEFEVRRFTR 120
           P +I   G  C + R      G+   Y+L +EYA  G+L D    +GG++ E  V ++TR
Sbjct: 61  PYVIGYRG--CEITREPFHNNGEATTYSLLMEYAPYGTLTDVATKNGGFIDEARVVKYTR 120

Query: 121 TILEGLRYIH-EKGFVHCDVKPRNILVFGDGDAKIADFGLSKKAGKNRAETGEEGKFQLR 180
            IL GL YIH  KG  HCD+K  N+LV  +G+AKIADFG +K       E        +R
Sbjct: 121 QILLGLEYIHNSKGIAHCDIKGSNVLVGENGEAKIADFGCAKWVEPEITE-------PVR 180

Query: 181 GSPLYMSPESVNDDEYESPCDIWAVGCAVVEMLTGKPAWNFPVECNIYSLLLKIGIGEEL 240
           G+P +M+PE+   +      DIWAVGC V+EM+TG   W      +  S+L ++G   EL
Sbjct: 181 GTPAFMAPEAARGERQGKESDIWAVGCTVIEMVTGSQPWIGADFTDPVSVLYRVGYLGEL 240

Query: 241 PKIPKYISEEGKDFLGKCLVKDPAKRWTAEMLLNHPFLADSGSSSVPLTPKGEPLTSPRC 300
           P++P  ++E+ KDFLGKCL K+  +RWTA  LLNHPFL     +  P    G    SP  
Sbjct: 241 PELPCSLTEQAKDFLGKCLKKEATERWTASQLLNHPFLV----NKEPELVTGLVTNSPTS 300

Query: 301 PFDFEDWASIHSLEMDQKFEEESNCWLDSSCPWSCSPTERLLQLVDNGPV----DWVIT- 355
             D   W S+     +++  E+ + W      W C   ER+  L   G V     W +  
Sbjct: 301 VTDQMFWRSV-----EEEVSEDRSSW------WECHEDERIGVLSWIGHVVVESTWDLDG 337

BLAST of CmaCh07G007010 vs. ExPASy Swiss-Prot
Match: O22042 (Mitogen-activated protein kinase kinase kinase 3 OS=Arabidopsis thaliana OX=3702 GN=ANP3 PE=1 SV=1)

HSP 1 Score: 174.9 bits (442), Expect = 1.7e-42
Identity = 103/302 (34.11%), Postives = 161/302 (53.31%), Query Frame = 0

Query: 1   MEWIRGHEIGCGSFATINLAMPSNTSNAQFPPLMAVKSSPAVSS--------ASLKNEKR 60
           + W +G  IGCG+F  + + M  N  + +   +  V  +P+ +S          L+ E +
Sbjct: 66  IRWRKGELIGCGAFGRVYMGM--NLDSGELLAIKQVLIAPSSASKEKTQGHIRELEEEVQ 125

Query: 61  VLDLLGDCPQIIRCFGESCSVERGDELYNLFLEYASGGSLADRIQSHGGWLPEFEVRRFT 120
           +L  L   P I+R  G   +V   D L N+ +E+  GGS++  ++  G + PE  +  +T
Sbjct: 126 LLKNLSH-PNIVRYLG---TVRESDSL-NILMEFVPGGSISSLLEKFGSF-PEPVIIMYT 185

Query: 121 RTILEGLRYIHEKGFVHCDVKPRNILVFGDGDAKIADFGLSKKAGKNRAETGEEGKFQLR 180
           + +L GL Y+H  G +H D+K  NILV   G  ++ADFG SKK  +     G +    ++
Sbjct: 186 KQLLLGLEYLHNNGIMHRDIKGANILVDNKGCIRLADFGASKKVVELATVNGAK---SMK 245

Query: 181 GSPLYMSPESVNDDEYESPCDIWAVGCAVVEMLTGKPAWNFPVECNIYSLLLKIGIGEEL 240
           G+P +M+PE +    +    DIW+VGC V+EM TGKP W+   +   ++ +L IG  +  
Sbjct: 246 GTPYWMAPEVILQTGHSFSADIWSVGCTVIEMATGKPPWS--EQYQQFAAVLHIGRTKAH 305

Query: 241 PKIPKYISEEGKDFLGKCLVKDPAKRWTAEMLLNHPFLADSGSSSVP-----LTPKGEPL 290
           P IP+ +S E KDFL KCL K+P+ R +A  LL HPF+        P     LT  G P+
Sbjct: 306 PPIPEDLSPEAKDFLMKCLHKEPSLRLSATELLQHPFVTGKRQEPYPAYRNSLTECGNPI 354

BLAST of CmaCh07G007010 vs. ExPASy Swiss-Prot
Match: Q40541 (Mitogen-activated protein kinase kinase kinase NPK1 OS=Nicotiana tabacum OX=4097 GN=NPK1 PE=1 SV=1)

HSP 1 Score: 174.9 bits (442), Expect = 1.7e-42
Identity = 103/290 (35.52%), Postives = 154/290 (53.10%), Query Frame = 0

Query: 1   MEWIRGHEIGCGSFATINLAMPSNTSNAQFPPLMAVKS-SPAVSSAS----------LKN 60
           + W +G  IGCG+F  + + M     N     L+A+K  S A++ AS          L+ 
Sbjct: 78  IRWRKGEMIGCGAFGRVYMGM-----NVDSGELLAIKEVSIAMNGASRERAQAHVRELEE 137

Query: 61  EKRVLDLLGDCPQIIRCFGESCSVERGDELYNLFLEYASGGSLADRIQSHGGWLPEFEVR 120
           E  +L  L   P I+R  G +    R     N+ LE+  GGS++  +   G + PE  +R
Sbjct: 138 EVNLLKNLSH-PNIVRYLGTA----REAGSLNILLEFVPGGSISSLLGKFGSF-PESVIR 197

Query: 121 RFTRTILEGLRYIHEKGFVHCDVKPRNILVFGDGDAKIADFGLSKKAGKNRAETGEEGKF 180
            +T+ +L GL Y+H+ G +H D+K  NILV   G  K+ADFG SKK  +    TG +   
Sbjct: 198 MYTKQLLLGLEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKKVVELATMTGAK--- 257

Query: 181 QLRGSPLYMSPESVNDDEYESPCDIWAVGCAVVEMLTGKPAWNFPVECNIYSLLLKIGIG 240
            ++G+P +M+PE +    +    DIW+VGC ++EM TGKP W+   +    + L  IG  
Sbjct: 258 SMKGTPYWMAPEVILQTGHSFSADIWSVGCTIIEMATGKPPWS--QQYQEVAALFHIGTT 317

Query: 241 EELPKIPKYISEEGKDFLGKCLVKDPAKRWTAEMLLNHPFLADSGSSSVP 280
           +  P IP+++S E KDFL KCL K+P  R +A  LL HPF+      + P
Sbjct: 318 KSHPPIPEHLSAESKDFLLKCLQKEPHLRHSASNLLQHPFVTAEHQEARP 351

BLAST of CmaCh07G007010 vs. ExPASy TrEMBL
Match: A0A6J1KQ57 (mitogen-activated protein kinase kinase kinase 18-like OS=Cucurbita maxima OX=3661 GN=LOC111497247 PE=3 SV=1)

HSP 1 Score: 753.1 bits (1943), Expect = 5.4e-214
Identity = 354/354 (100.00%), Postives = 354/354 (100.00%), Query Frame = 0

Query: 1   MEWIRGHEIGCGSFATINLAMPSNTSNAQFPPLMAVKSSPAVSSASLKNEKRVLDLLGDC 60
           MEWIRGHEIGCGSFATINLAMPSNTSNAQFPPLMAVKSSPAVSSASLKNEKRVLDLLGDC
Sbjct: 1   MEWIRGHEIGCGSFATINLAMPSNTSNAQFPPLMAVKSSPAVSSASLKNEKRVLDLLGDC 60

Query: 61  PQIIRCFGESCSVERGDELYNLFLEYASGGSLADRIQSHGGWLPEFEVRRFTRTILEGLR 120
           PQIIRCFGESCSVERGDELYNLFLEYASGGSLADRIQSHGGWLPEFEVRRFTRTILEGLR
Sbjct: 61  PQIIRCFGESCSVERGDELYNLFLEYASGGSLADRIQSHGGWLPEFEVRRFTRTILEGLR 120

Query: 121 YIHEKGFVHCDVKPRNILVFGDGDAKIADFGLSKKAGKNRAETGEEGKFQLRGSPLYMSP 180
           YIHEKGFVHCDVKPRNILVFGDGDAKIADFGLSKKAGKNRAETGEEGKFQLRGSPLYMSP
Sbjct: 121 YIHEKGFVHCDVKPRNILVFGDGDAKIADFGLSKKAGKNRAETGEEGKFQLRGSPLYMSP 180

Query: 181 ESVNDDEYESPCDIWAVGCAVVEMLTGKPAWNFPVECNIYSLLLKIGIGEELPKIPKYIS 240
           ESVNDDEYESPCDIWAVGCAVVEMLTGKPAWNFPVECNIYSLLLKIGIGEELPKIPKYIS
Sbjct: 181 ESVNDDEYESPCDIWAVGCAVVEMLTGKPAWNFPVECNIYSLLLKIGIGEELPKIPKYIS 240

Query: 241 EEGKDFLGKCLVKDPAKRWTAEMLLNHPFLADSGSSSVPLTPKGEPLTSPRCPFDFEDWA 300
           EEGKDFLGKCLVKDPAKRWTAEMLLNHPFLADSGSSSVPLTPKGEPLTSPRCPFDFEDWA
Sbjct: 241 EEGKDFLGKCLVKDPAKRWTAEMLLNHPFLADSGSSSVPLTPKGEPLTSPRCPFDFEDWA 300

Query: 301 SIHSLEMDQKFEEESNCWLDSSCPWSCSPTERLLQLVDNGPVDWVITDSWVTVR 355
           SIHSLEMDQKFEEESNCWLDSSCPWSCSPTERLLQLVDNGPVDWVITDSWVTVR
Sbjct: 301 SIHSLEMDQKFEEESNCWLDSSCPWSCSPTERLLQLVDNGPVDWVITDSWVTVR 354

BLAST of CmaCh07G007010 vs. ExPASy TrEMBL
Match: A0A6J1HG32 (mitogen-activated protein kinase kinase kinase 17-like OS=Cucurbita moschata OX=3662 GN=LOC111463177 PE=3 SV=1)

HSP 1 Score: 729.9 bits (1883), Expect = 4.9e-207
Identity = 343/354 (96.89%), Postives = 348/354 (98.31%), Query Frame = 0

Query: 1   MEWIRGHEIGCGSFATINLAMPSNTSNAQFPPLMAVKSSPAVSSASLKNEKRVLDLLGDC 60
           M+WIRGHEIGCGSFATINLAMPSNTSNAQFPPLMAVKSSPAVSSASLKNEKRVLDLLGDC
Sbjct: 1   MDWIRGHEIGCGSFATINLAMPSNTSNAQFPPLMAVKSSPAVSSASLKNEKRVLDLLGDC 60

Query: 61  PQIIRCFGESCSVERGDELYNLFLEYASGGSLADRIQSHGGWLPEFEVRRFTRTILEGLR 120
           PQIIRCFGESCSVERGDELYNLFLEYASGGSLADRIQSHGGWLPEFEVRR TRTILEGLR
Sbjct: 61  PQIIRCFGESCSVERGDELYNLFLEYASGGSLADRIQSHGGWLPEFEVRRLTRTILEGLR 120

Query: 121 YIHEKGFVHCDVKPRNILVFGDGDAKIADFGLSKKAGKNRAETGEEGKFQLRGSPLYMSP 180
           YIHEKGFVHCDVKPRNILVFGDG+AKIADFGLSKKAGKNRAETGEEGKFQLRGSPLYMSP
Sbjct: 121 YIHEKGFVHCDVKPRNILVFGDGEAKIADFGLSKKAGKNRAETGEEGKFQLRGSPLYMSP 180

Query: 181 ESVNDDEYESPCDIWAVGCAVVEMLTGKPAWNFPVECNIYSLLLKIGIGEELPKIPKYIS 240
           ESVND+EYESPCDIWAVGCAVVEMLTGKPAWNFP+E NIYSLLLKIGIGEELPKIPKYIS
Sbjct: 181 ESVNDNEYESPCDIWAVGCAVVEMLTGKPAWNFPMESNIYSLLLKIGIGEELPKIPKYIS 240

Query: 241 EEGKDFLGKCLVKDPAKRWTAEMLLNHPFLADSGSSSVPLTPKGEPLTSPRCPFDFEDWA 300
           EEGKDFLGKCLVKDPAKRWTAEMLLNHPFLADSGSSSVPLTPK EP TSPRCPFDFEDWA
Sbjct: 241 EEGKDFLGKCLVKDPAKRWTAEMLLNHPFLADSGSSSVPLTPKDEPSTSPRCPFDFEDWA 300

Query: 301 SIHSLEMDQKFEEESNCWLDSSCPWSCSPTERLLQLVDNGPVDWVITDSWVTVR 355
           SIHS E+DQKFEEESNCWLDSSCPWSCSPTERLLQLVD GPVDWVITDSWVTVR
Sbjct: 301 SIHSQEVDQKFEEESNCWLDSSCPWSCSPTERLLQLVDKGPVDWVITDSWVTVR 354

BLAST of CmaCh07G007010 vs. ExPASy TrEMBL
Match: A0A5D3CM36 (Mitogen-activated protein kinase kinase kinase 3-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G004750 PE=3 SV=1)

HSP 1 Score: 585.5 bits (1508), Expect = 1.5e-163
Identity = 286/357 (80.11%), Postives = 311/357 (87.11%), Query Frame = 0

Query: 1   MEWIRGHEIGCGSFATINLAMPSNTSNAQFPPLMAVKSSPAVSSASLKNEKRVLDLLG-D 60
           M+W+RGHEIG GSFATINLA PS  S  +FPPLMAVKSS  V S+SLKNEK++LDLLG D
Sbjct: 1   MDWVRGHEIGHGSFATINLATPS--SGDRFPPLMAVKSSELVCSSSLKNEKQILDLLGDD 60

Query: 61  CPQIIRCFGESCSVERGDELYNLFLEYASGGSLADRIQSHGGWLPEFEVRRFTRTILEGL 120
           CPQIIRCFGESCSVE G+ELYNLFLEYA+GGSLADRIQS GG LPEFEVRR+TRTILEGL
Sbjct: 61  CPQIIRCFGESCSVENGEELYNLFLEYATGGSLADRIQSCGGRLPEFEVRRYTRTILEGL 120

Query: 121 RYIHEKGFVHCDVKPRNILVFGDGDAKIADFGLSKKAGKNRAETGEE-GKFQLRGSPLYM 180
           RYIH KGFVHCDVKPRNILVFGDGDAKIADFGLSKKAGKNR ETGEE G+FQLRGSPLYM
Sbjct: 121 RYIHGKGFVHCDVKPRNILVFGDGDAKIADFGLSKKAGKNRVETGEETGRFQLRGSPLYM 180

Query: 181 SPESVNDDEYESPCDIWAVGCAVVEMLTGKPAWNFPVECNIYSLLLKIGIGEELPKIPKY 240
           SPES+ND+EYESPCDIWAVGCAVVEM TGKPAWN   E N+++LL+KIGIGEELP+IPK 
Sbjct: 181 SPESLNDNEYESPCDIWAVGCAVVEMFTGKPAWNCRPESNVFALLIKIGIGEELPEIPKD 240

Query: 241 ISEEGKDFLGKCLVKDPAKRWTAEMLLNHPFLADSGSSSVPLTPKGEPLTSPRCPFDFED 300
           +SEEGKDFL KCLVKDP KRWTA+MLL HPF+ADSG   VPL    E  TSPRCPFDFED
Sbjct: 241 LSEEGKDFLRKCLVKDPVKRWTADMLLKHPFVADSG-RCVPLGEMDEVSTSPRCPFDFED 300

Query: 301 WASIHSLEMDQKFEEESNCWLDSSCPW-SCSPTERLLQLVDNGPVDWVITDSWVTVR 355
           WASIHS E D + EEESNCWL+SSC W S SP ERLLQLV+ G VDW +TD+WV VR
Sbjct: 301 WASIHSQERDPRNEEESNCWLNSSCSWSSSSPRERLLQLVEKGAVDWSVTDNWVRVR 354

BLAST of CmaCh07G007010 vs. ExPASy TrEMBL
Match: A0A1S3B1I0 (mitogen-activated protein kinase kinase kinase 3-like OS=Cucumis melo OX=3656 GN=LOC103484781 PE=3 SV=1)

HSP 1 Score: 585.5 bits (1508), Expect = 1.5e-163
Identity = 286/357 (80.11%), Postives = 311/357 (87.11%), Query Frame = 0

Query: 1   MEWIRGHEIGCGSFATINLAMPSNTSNAQFPPLMAVKSSPAVSSASLKNEKRVLDLLG-D 60
           M+W+RGHEIG GSFATINLA PS  S  +FPPLMAVKSS  V S+SLKNEK++LDLLG D
Sbjct: 1   MDWVRGHEIGHGSFATINLATPS--SGDRFPPLMAVKSSELVCSSSLKNEKQILDLLGDD 60

Query: 61  CPQIIRCFGESCSVERGDELYNLFLEYASGGSLADRIQSHGGWLPEFEVRRFTRTILEGL 120
           CPQIIRCFGESCSVE G+ELYNLFLEYA+GGSLADRIQS GG LPEFEVRR+TRTILEGL
Sbjct: 61  CPQIIRCFGESCSVENGEELYNLFLEYATGGSLADRIQSCGGRLPEFEVRRYTRTILEGL 120

Query: 121 RYIHEKGFVHCDVKPRNILVFGDGDAKIADFGLSKKAGKNRAETGEE-GKFQLRGSPLYM 180
           RYIH KGFVHCDVKPRNILVFGDGDAKIADFGLSKKAGKNR ETGEE G+FQLRGSPLYM
Sbjct: 121 RYIHGKGFVHCDVKPRNILVFGDGDAKIADFGLSKKAGKNRVETGEETGRFQLRGSPLYM 180

Query: 181 SPESVNDDEYESPCDIWAVGCAVVEMLTGKPAWNFPVECNIYSLLLKIGIGEELPKIPKY 240
           SPES+ND+EYESPCDIWAVGCAVVEM TGKPAWN   E N+++LL+KIGIGEELP+IPK 
Sbjct: 181 SPESLNDNEYESPCDIWAVGCAVVEMFTGKPAWNCRPESNVFALLIKIGIGEELPEIPKD 240

Query: 241 ISEEGKDFLGKCLVKDPAKRWTAEMLLNHPFLADSGSSSVPLTPKGEPLTSPRCPFDFED 300
           +SEEGKDFL KCLVKDP KRWTA+MLL HPF+ADSG   VPL    E  TSPRCPFDFED
Sbjct: 241 LSEEGKDFLRKCLVKDPVKRWTADMLLKHPFVADSG-RCVPLGEMDEVSTSPRCPFDFED 300

Query: 301 WASIHSLEMDQKFEEESNCWLDSSCPW-SCSPTERLLQLVDNGPVDWVITDSWVTVR 355
           WASIHS E D + EEESNCWL+SSC W S SP ERLLQLV+ G VDW +TD+WV VR
Sbjct: 301 WASIHSQERDPRNEEESNCWLNSSCSWSSSSPRERLLQLVEKGAVDWSVTDNWVRVR 354

BLAST of CmaCh07G007010 vs. ExPASy TrEMBL
Match: A0A0A0KLC9 (Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G490950 PE=3 SV=1)

HSP 1 Score: 574.7 bits (1480), Expect = 2.6e-160
Identity = 282/356 (79.21%), Postives = 309/356 (86.80%), Query Frame = 0

Query: 1   MEWIRGHEIGCGSFATINLAMPSNTSNAQFPPLMAVKSSPAVSSASLKNEKRVLDLLG-D 60
           M+W+RG EIG GSFATINLA  ++ S  +FPPLMAVKSS  V SASLKNEK+VLDLLG D
Sbjct: 1   MDWVRGDEIGYGSFATINLA--TSCSGDRFPPLMAVKSSGLVCSASLKNEKQVLDLLGDD 60

Query: 61  CPQIIRCFGESCSVERGDELYNLFLEYASGGSLADRIQSHGGWLPEFEVRRFTRTILEGL 120
           CPQIIRCFG+SCSVE G+ELYNLFLEYASGGSLADRIQS GG LPEFEVRR+TRTILEGL
Sbjct: 61  CPQIIRCFGDSCSVENGEELYNLFLEYASGGSLADRIQSRGGRLPEFEVRRYTRTILEGL 120

Query: 121 RYIHEKGFVHCDVKPRNILVFGDGDAKIADFGLSKKAGKNRAETGEE-GKFQLRGSPLYM 180
           RYIH KGFVHCDVKPRNILVFGDGDAKIADFGLSKKAGKNR  TGEE GKFQLRGSPLYM
Sbjct: 121 RYIHGKGFVHCDVKPRNILVFGDGDAKIADFGLSKKAGKNRVGTGEETGKFQLRGSPLYM 180

Query: 181 SPESVNDDEYESPCDIWAVGCAVVEMLTGKPAWNFPVECNIYSLLLKIGIGEELPKIPKY 240
           SPESVND+EYESPCDIWAVGCAVVEMLTGKPAWN   E N+++LL+KIGIGEELP+IPK 
Sbjct: 181 SPESVNDNEYESPCDIWAVGCAVVEMLTGKPAWNCRPESNVFALLIKIGIGEELPEIPKD 240

Query: 241 ISEEGKDFLGKCLVKDPAKRWTAEMLLNHPFLADSGSSSVPLTPKGEPLTSPRCPFDFED 300
           +SEEGKDFL KCLVKDP KRWTA+MLL HPF+A+SG   VPL    E  TSPRCPFDFED
Sbjct: 241 LSEEGKDFLKKCLVKDPLKRWTADMLLKHPFVAESG-RCVPLAGVEEVSTSPRCPFDFED 300

Query: 301 WASIHSLEMDQKFEEESNCWLDSSCPWSCSPTERLLQLVDNGPVDWVITDSWVTVR 355
           WASIHS E D + EEE+NCWL++   WSCSP ERLL+LV NG V+W +TD+WV VR
Sbjct: 301 WASIHSQESDPRNEEEANCWLNN---WSCSPRERLLELVGNGAVEWSVTDNWVRVR 350

BLAST of CmaCh07G007010 vs. NCBI nr
Match: XP_023003761.1 (mitogen-activated protein kinase kinase kinase 18-like [Cucurbita maxima])

HSP 1 Score: 753.1 bits (1943), Expect = 1.1e-213
Identity = 354/354 (100.00%), Postives = 354/354 (100.00%), Query Frame = 0

Query: 1   MEWIRGHEIGCGSFATINLAMPSNTSNAQFPPLMAVKSSPAVSSASLKNEKRVLDLLGDC 60
           MEWIRGHEIGCGSFATINLAMPSNTSNAQFPPLMAVKSSPAVSSASLKNEKRVLDLLGDC
Sbjct: 1   MEWIRGHEIGCGSFATINLAMPSNTSNAQFPPLMAVKSSPAVSSASLKNEKRVLDLLGDC 60

Query: 61  PQIIRCFGESCSVERGDELYNLFLEYASGGSLADRIQSHGGWLPEFEVRRFTRTILEGLR 120
           PQIIRCFGESCSVERGDELYNLFLEYASGGSLADRIQSHGGWLPEFEVRRFTRTILEGLR
Sbjct: 61  PQIIRCFGESCSVERGDELYNLFLEYASGGSLADRIQSHGGWLPEFEVRRFTRTILEGLR 120

Query: 121 YIHEKGFVHCDVKPRNILVFGDGDAKIADFGLSKKAGKNRAETGEEGKFQLRGSPLYMSP 180
           YIHEKGFVHCDVKPRNILVFGDGDAKIADFGLSKKAGKNRAETGEEGKFQLRGSPLYMSP
Sbjct: 121 YIHEKGFVHCDVKPRNILVFGDGDAKIADFGLSKKAGKNRAETGEEGKFQLRGSPLYMSP 180

Query: 181 ESVNDDEYESPCDIWAVGCAVVEMLTGKPAWNFPVECNIYSLLLKIGIGEELPKIPKYIS 240
           ESVNDDEYESPCDIWAVGCAVVEMLTGKPAWNFPVECNIYSLLLKIGIGEELPKIPKYIS
Sbjct: 181 ESVNDDEYESPCDIWAVGCAVVEMLTGKPAWNFPVECNIYSLLLKIGIGEELPKIPKYIS 240

Query: 241 EEGKDFLGKCLVKDPAKRWTAEMLLNHPFLADSGSSSVPLTPKGEPLTSPRCPFDFEDWA 300
           EEGKDFLGKCLVKDPAKRWTAEMLLNHPFLADSGSSSVPLTPKGEPLTSPRCPFDFEDWA
Sbjct: 241 EEGKDFLGKCLVKDPAKRWTAEMLLNHPFLADSGSSSVPLTPKGEPLTSPRCPFDFEDWA 300

Query: 301 SIHSLEMDQKFEEESNCWLDSSCPWSCSPTERLLQLVDNGPVDWVITDSWVTVR 355
           SIHSLEMDQKFEEESNCWLDSSCPWSCSPTERLLQLVDNGPVDWVITDSWVTVR
Sbjct: 301 SIHSLEMDQKFEEESNCWLDSSCPWSCSPTERLLQLVDNGPVDWVITDSWVTVR 354

BLAST of CmaCh07G007010 vs. NCBI nr
Match: XP_023517683.1 (mitogen-activated protein kinase kinase kinase 17-like [Cucurbita pepo subsp. pepo] >XP_023522778.1 mitogen-activated protein kinase kinase kinase 17-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 731.5 bits (1887), Expect = 3.5e-207
Identity = 344/354 (97.18%), Postives = 348/354 (98.31%), Query Frame = 0

Query: 1   MEWIRGHEIGCGSFATINLAMPSNTSNAQFPPLMAVKSSPAVSSASLKNEKRVLDLLGDC 60
           M+WIRGHEIGCGSFATINLAMPSNTS AQFPPLMAVKSSPAVSSASLKNEKRVLDLLGDC
Sbjct: 1   MDWIRGHEIGCGSFATINLAMPSNTSTAQFPPLMAVKSSPAVSSASLKNEKRVLDLLGDC 60

Query: 61  PQIIRCFGESCSVERGDELYNLFLEYASGGSLADRIQSHGGWLPEFEVRRFTRTILEGLR 120
           PQIIRCFGESCSVERGDELYNLFLEYASGGSLADRIQSHGGWLPEFEVRR TRTILEGLR
Sbjct: 61  PQIIRCFGESCSVERGDELYNLFLEYASGGSLADRIQSHGGWLPEFEVRRLTRTILEGLR 120

Query: 121 YIHEKGFVHCDVKPRNILVFGDGDAKIADFGLSKKAGKNRAETGEEGKFQLRGSPLYMSP 180
           YIHEKGFVHCDVKPRNILVFGDGDAKIADFGLSKKAGKNRAETGEEGKFQLRGSPLYMSP
Sbjct: 121 YIHEKGFVHCDVKPRNILVFGDGDAKIADFGLSKKAGKNRAETGEEGKFQLRGSPLYMSP 180

Query: 181 ESVNDDEYESPCDIWAVGCAVVEMLTGKPAWNFPVECNIYSLLLKIGIGEELPKIPKYIS 240
           ESVND+EYESPCDIWAVGCAVVEMLTGKPAWNFP+E NIYSLLLKIGIGEELPKIPKYIS
Sbjct: 181 ESVNDNEYESPCDIWAVGCAVVEMLTGKPAWNFPMESNIYSLLLKIGIGEELPKIPKYIS 240

Query: 241 EEGKDFLGKCLVKDPAKRWTAEMLLNHPFLADSGSSSVPLTPKGEPLTSPRCPFDFEDWA 300
           EEGKDFLGKCLVKDPAKRWTAEMLLNHPFLADSGSSSVPLTPK EP TSPRCPFDFEDWA
Sbjct: 241 EEGKDFLGKCLVKDPAKRWTAEMLLNHPFLADSGSSSVPLTPKDEPSTSPRCPFDFEDWA 300

Query: 301 SIHSLEMDQKFEEESNCWLDSSCPWSCSPTERLLQLVDNGPVDWVITDSWVTVR 355
           SIHS E+DQKFEEESNCWLDSSCPWSCSPTERLLQLVDNGPVDWVITDSWVTVR
Sbjct: 301 SIHSQEVDQKFEEESNCWLDSSCPWSCSPTERLLQLVDNGPVDWVITDSWVTVR 354

BLAST of CmaCh07G007010 vs. NCBI nr
Match: KAG6595031.1 (Mitogen-activated protein kinase kinase kinase 20, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 731.1 bits (1886), Expect = 4.6e-207
Identity = 343/354 (96.89%), Postives = 349/354 (98.59%), Query Frame = 0

Query: 1   MEWIRGHEIGCGSFATINLAMPSNTSNAQFPPLMAVKSSPAVSSASLKNEKRVLDLLGDC 60
           M+WIRGHEIGCGSFATINLAMPSNTSNAQFPPLMAVKSSPAVSSASLKNEKRVLDLLGDC
Sbjct: 1   MDWIRGHEIGCGSFATINLAMPSNTSNAQFPPLMAVKSSPAVSSASLKNEKRVLDLLGDC 60

Query: 61  PQIIRCFGESCSVERGDELYNLFLEYASGGSLADRIQSHGGWLPEFEVRRFTRTILEGLR 120
           PQIIRCFGESCSVERGDELYNLFLEYASGGSLADRIQSHGGWLPEFEVRR TRTILEGLR
Sbjct: 61  PQIIRCFGESCSVERGDELYNLFLEYASGGSLADRIQSHGGWLPEFEVRRLTRTILEGLR 120

Query: 121 YIHEKGFVHCDVKPRNILVFGDGDAKIADFGLSKKAGKNRAETGEEGKFQLRGSPLYMSP 180
           YIH+KGFVHCDVKPRNILVFGDG+AKIADFGLSKKAGKNRAETGEEGKFQLRGSPLYMSP
Sbjct: 121 YIHDKGFVHCDVKPRNILVFGDGEAKIADFGLSKKAGKNRAETGEEGKFQLRGSPLYMSP 180

Query: 181 ESVNDDEYESPCDIWAVGCAVVEMLTGKPAWNFPVECNIYSLLLKIGIGEELPKIPKYIS 240
           ESVND+EYESPCDIWAVGCAVVEMLTGKPAWNFP+E NIYSLLLKIGIGEELPKIPKYIS
Sbjct: 181 ESVNDNEYESPCDIWAVGCAVVEMLTGKPAWNFPMESNIYSLLLKIGIGEELPKIPKYIS 240

Query: 241 EEGKDFLGKCLVKDPAKRWTAEMLLNHPFLADSGSSSVPLTPKGEPLTSPRCPFDFEDWA 300
           EEGKDFLGKCLVKDPAKRWTAEMLLNHPFLADSGSSSVPLTPK EP TSPRCPFDFEDWA
Sbjct: 241 EEGKDFLGKCLVKDPAKRWTAEMLLNHPFLADSGSSSVPLTPKDEPSTSPRCPFDFEDWA 300

Query: 301 SIHSLEMDQKFEEESNCWLDSSCPWSCSPTERLLQLVDNGPVDWVITDSWVTVR 355
           SIHS E+DQKFEEESNCWLDSSCPWSCSPTERLLQLVDNGPVDWVITDSWVTVR
Sbjct: 301 SIHSQEVDQKFEEESNCWLDSSCPWSCSPTERLLQLVDNGPVDWVITDSWVTVR 354

BLAST of CmaCh07G007010 vs. NCBI nr
Match: XP_022962795.1 (mitogen-activated protein kinase kinase kinase 17-like [Cucurbita moschata])

HSP 1 Score: 729.9 bits (1883), Expect = 1.0e-206
Identity = 343/354 (96.89%), Postives = 348/354 (98.31%), Query Frame = 0

Query: 1   MEWIRGHEIGCGSFATINLAMPSNTSNAQFPPLMAVKSSPAVSSASLKNEKRVLDLLGDC 60
           M+WIRGHEIGCGSFATINLAMPSNTSNAQFPPLMAVKSSPAVSSASLKNEKRVLDLLGDC
Sbjct: 1   MDWIRGHEIGCGSFATINLAMPSNTSNAQFPPLMAVKSSPAVSSASLKNEKRVLDLLGDC 60

Query: 61  PQIIRCFGESCSVERGDELYNLFLEYASGGSLADRIQSHGGWLPEFEVRRFTRTILEGLR 120
           PQIIRCFGESCSVERGDELYNLFLEYASGGSLADRIQSHGGWLPEFEVRR TRTILEGLR
Sbjct: 61  PQIIRCFGESCSVERGDELYNLFLEYASGGSLADRIQSHGGWLPEFEVRRLTRTILEGLR 120

Query: 121 YIHEKGFVHCDVKPRNILVFGDGDAKIADFGLSKKAGKNRAETGEEGKFQLRGSPLYMSP 180
           YIHEKGFVHCDVKPRNILVFGDG+AKIADFGLSKKAGKNRAETGEEGKFQLRGSPLYMSP
Sbjct: 121 YIHEKGFVHCDVKPRNILVFGDGEAKIADFGLSKKAGKNRAETGEEGKFQLRGSPLYMSP 180

Query: 181 ESVNDDEYESPCDIWAVGCAVVEMLTGKPAWNFPVECNIYSLLLKIGIGEELPKIPKYIS 240
           ESVND+EYESPCDIWAVGCAVVEMLTGKPAWNFP+E NIYSLLLKIGIGEELPKIPKYIS
Sbjct: 181 ESVNDNEYESPCDIWAVGCAVVEMLTGKPAWNFPMESNIYSLLLKIGIGEELPKIPKYIS 240

Query: 241 EEGKDFLGKCLVKDPAKRWTAEMLLNHPFLADSGSSSVPLTPKGEPLTSPRCPFDFEDWA 300
           EEGKDFLGKCLVKDPAKRWTAEMLLNHPFLADSGSSSVPLTPK EP TSPRCPFDFEDWA
Sbjct: 241 EEGKDFLGKCLVKDPAKRWTAEMLLNHPFLADSGSSSVPLTPKDEPSTSPRCPFDFEDWA 300

Query: 301 SIHSLEMDQKFEEESNCWLDSSCPWSCSPTERLLQLVDNGPVDWVITDSWVTVR 355
           SIHS E+DQKFEEESNCWLDSSCPWSCSPTERLLQLVD GPVDWVITDSWVTVR
Sbjct: 301 SIHSQEVDQKFEEESNCWLDSSCPWSCSPTERLLQLVDKGPVDWVITDSWVTVR 354

BLAST of CmaCh07G007010 vs. NCBI nr
Match: KAG7027055.1 (Mitogen-activated protein kinase kinase kinase 17, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 728.0 bits (1878), Expect = 3.9e-206
Identity = 342/354 (96.61%), Postives = 348/354 (98.31%), Query Frame = 0

Query: 1   MEWIRGHEIGCGSFATINLAMPSNTSNAQFPPLMAVKSSPAVSSASLKNEKRVLDLLGDC 60
           M+WIRGHEIGCGSFATINLAMPSNTSNAQFPPLMAVKSSPAVSSASLKNEKRVLDLLGDC
Sbjct: 1   MDWIRGHEIGCGSFATINLAMPSNTSNAQFPPLMAVKSSPAVSSASLKNEKRVLDLLGDC 60

Query: 61  PQIIRCFGESCSVERGDELYNLFLEYASGGSLADRIQSHGGWLPEFEVRRFTRTILEGLR 120
           PQIIRCFGESCSVERGDELYNLFLEYASGGSLADRIQSHGGWLPEFEVRR TRTILEGLR
Sbjct: 61  PQIIRCFGESCSVERGDELYNLFLEYASGGSLADRIQSHGGWLPEFEVRRLTRTILEGLR 120

Query: 121 YIHEKGFVHCDVKPRNILVFGDGDAKIADFGLSKKAGKNRAETGEEGKFQLRGSPLYMSP 180
           YIH+KGFVHCDVKPRNILVFGDG+AKIADFGLSKKAGKNRAETGEEGKFQLRGSPLYMSP
Sbjct: 121 YIHDKGFVHCDVKPRNILVFGDGEAKIADFGLSKKAGKNRAETGEEGKFQLRGSPLYMSP 180

Query: 181 ESVNDDEYESPCDIWAVGCAVVEMLTGKPAWNFPVECNIYSLLLKIGIGEELPKIPKYIS 240
           ESVND+EYESPCDIWAVGCAVVEMLTGKPAWNF +E NIYSLLLKIGIGEELPKIPKYIS
Sbjct: 181 ESVNDNEYESPCDIWAVGCAVVEMLTGKPAWNFSMESNIYSLLLKIGIGEELPKIPKYIS 240

Query: 241 EEGKDFLGKCLVKDPAKRWTAEMLLNHPFLADSGSSSVPLTPKGEPLTSPRCPFDFEDWA 300
           EEGKDFLGKCLVKDPAKRWTAEMLLNHPFLADSGSSSVPLTPK EP TSPRCPFDFEDWA
Sbjct: 241 EEGKDFLGKCLVKDPAKRWTAEMLLNHPFLADSGSSSVPLTPKDEPSTSPRCPFDFEDWA 300

Query: 301 SIHSLEMDQKFEEESNCWLDSSCPWSCSPTERLLQLVDNGPVDWVITDSWVTVR 355
           SIHS E+DQKFEEESNCWLDSSCPWSCSPTERLLQLVDNGPVDWVITDSWVTVR
Sbjct: 301 SIHSQEVDQKFEEESNCWLDSSCPWSCSPTERLLQLVDNGPVDWVITDSWVTVR 354

BLAST of CmaCh07G007010 vs. TAIR 10
Match: AT4G36950.1 (mitogen-activated protein kinase kinase kinase 21 )

HSP 1 Score: 374.4 bits (960), Expect = 1.0e-103
Identity = 195/359 (54.32%), Postives = 243/359 (67.69%), Query Frame = 0

Query: 1   MEWIRGHEIGCGSFATINLAMPSNTSNAQFPPLMAVKSSPAVSSASLKNEKRVLDLLGDC 60
           MEWIR   IG GSF+T++LA  S +S+  FP LMAVKSS  V SA+L+NE+ VLD LGDC
Sbjct: 1   MEWIRRETIGHGSFSTVSLATTSGSSSKAFPSLMAVKSSGVVCSAALRNERDVLDDLGDC 60

Query: 61  PQIIRCFGESCSVERGDELYNLFLEYASGGSLADRIQSHGGWLPEFEVRRFTRTILEGLR 120
            +I+RCFGE  +VE G+E+YNLFLEYASGGSLADRI+S G  LPEFEVRRFTR+I++GL 
Sbjct: 61  SEIVRCFGEGRTVENGEEIYNLFLEYASGGSLADRIKSSGEALPEFEVRRFTRSIVKGLC 120

Query: 121 YIHEKGFVHCDVKPRNILVFGDGDAKIADFGLSKKAGKNRAETGEEGKFQLRGSPLYMSP 180
           +IH  GF HCD+K  N+LVFGDGD KI+DFGL+K+          E   ++RG+PLYM+P
Sbjct: 121 HIHGNGFTHCDIKLENVLVFGDGDVKISDFGLAKR-------RSGEVCVEIRGTPLYMAP 180

Query: 181 ESVNDDEYESPCDIWAVGCAVVEMLTGKPAWNFP--VECNIYSLLLKIGIGEELPKIPKY 240
           ESVN  E+ESP DIWA+GC+VVEM +GK AW     V  N+ SLL++IG G+E+P+IP  
Sbjct: 181 ESVNHGEFESPADIWALGCSVVEMSSGKTAWCLEDGVMNNVMSLLVRIGSGDEVPRIPVE 240

Query: 241 ISEEGKDFLGKCLVKDPAKRWTAEMLLNHPFLA---DSGSSSVPLTPKGEPLTSPRCPFD 300
           +SEEGKDF+ KC VK+ A+RWTAEMLL+HPFLA   +SG      +       SPR PFD
Sbjct: 241 LSEEGKDFVSKCFVKNAAERWTAEMLLDHPFLAVDDESGEEDEACS------VSPRNPFD 300

Query: 301 FEDWASIHSLEMDQKFEEESNCWLDSSCPWSCSPTERLLQLVDNGPVDWVITDSWVTVR 355
           F  W S+ S   D                   SP ER+  LV     DW ++  WV VR
Sbjct: 301 FPGWNSVQSPVNDSVMFGS----------LVGSPEERISGLVSEKVPDWSVSCDWVNVR 336

BLAST of CmaCh07G007010 vs. TAIR 10
Match: AT3G50310.1 (mitogen-activated protein kinase kinase kinase 20 )

HSP 1 Score: 367.9 bits (943), Expect = 9.5e-102
Identity = 191/361 (52.91%), Postives = 239/361 (66.20%), Query Frame = 0

Query: 1   MEWIRGHEIGCGSFATINLAMPSNTSNAQFPPLMAVKSSPAVSSASLKNEKRVLDLLGDC 60
           MEW+RG  IG G+F+T++ A  S  S   FP L+AVKS+ A  +ASL NEK VLD LGDC
Sbjct: 1   MEWVRGETIGFGTFSTVSTATKSRNS-GDFPALIAVKSTDAYGAASLSNEKSVLDSLGDC 60

Query: 61  PQIIRCFGESCSVERGDELYNLFLEYASGGSLADRIQSHGG-WLPEFEVRRFTRTILEGL 120
           P+IIRC+GE  +VE G+E++NL LEYAS GSLA  ++  GG  LPE  VRR T ++L GL
Sbjct: 61  PEIIRCYGEDSTVENGEEMHNLLLEYASRGSLASYMKKLGGEGLPESTVRRHTGSVLRGL 120

Query: 121 RYIHEKGFVHCDVKPRNILVFGDGDAKIADFGLSKKAGKNRAETGEEGKFQLRGSPLYMS 180
           R+IH KGF HCD+K  NIL+F DG  KIADFGL+ +   +   T      ++RG+PLYM+
Sbjct: 121 RHIHAKGFAHCDIKLANILLFNDGSVKIADFGLAMRVDGDL--TALRKSVEIRGTPLYMA 180

Query: 181 PESVNDDEYESPCDIWAVGCAVVEMLTGKPAWNFPVECNIYSLLLKIGIGEELPKIPKYI 240
           PE VND+EY S  D+WA+GCAVVEM +GK AW+     +  SLL++IG+G+ELPKIP+ +
Sbjct: 181 PECVNDNEYGSAADVWALGCAVVEMFSGKTAWSVKEGSHFMSLLIRIGVGDELPKIPEML 240

Query: 241 SEEGKDFLGKCLVKDPAKRWTAEMLLNHPF----LADSGSSSVPLTPKGEP--LTSPRCP 300
           SEEGKDFL KC VKDPAKRWTAEMLLNH F    L D    +  +  K E   L SP+CP
Sbjct: 241 SEEGKDFLSKCFVKDPAKRWTAEMLLNHSFVTIDLEDDHRENFVVKVKDEDKVLMSPKCP 300

Query: 301 FDFEDWASIHSLEMDQKFEEESNCWLDSSCPWSCSPTERLLQLVDNGPVDWVITDSWVTV 355
           F+F+DW S         F  +SN   D       SP ERL  LV     DW +  SW+TV
Sbjct: 301 FEFDDWDS---------FTLDSNPSFD-------SPVERLGSLVSGSIPDWSVGGSWLTV 342

BLAST of CmaCh07G007010 vs. TAIR 10
Match: AT5G67080.1 (mitogen-activated protein kinase kinase kinase 19 )

HSP 1 Score: 367.9 bits (943), Expect = 9.5e-102
Identity = 189/364 (51.92%), Postives = 242/364 (66.48%), Query Frame = 0

Query: 1   MEWIRGHEIGCGSFATINLAMPSNTSNAQFPPLMAVKSSPAVSSASLKNEKRVLDLLG-D 60
           MEWIRG  IG G+F+T++LA  SN  + +FPPLMAVKS+ +  +ASL NEK VLD LG D
Sbjct: 1   MEWIRGETIGYGTFSTVSLATRSNNDSGEFPPLMAVKSADSYGAASLANEKSVLDNLGDD 60

Query: 61  CPQIIRCFGESCSVERGDELYNLFLEYASGGSLADRIQSHGG-WLPEFEVRRFTRTILEG 120
           C +I+RCFGE  +VE G+E++NLFLEYAS GSL   ++   G  +PE  VRR T ++L G
Sbjct: 61  CNEIVRCFGEDRTVENGEEMHNLFLEYASRGSLESYLKKLAGEGVPESTVRRHTGSVLRG 120

Query: 121 LRYIHEKGFVHCDVKPRNILVFGDGDAKIADFGLSKKAGKNRAETGEEGKFQLRGSPLYM 180
           LR+IH  GF HCD+K  NIL+FGDG  KIADFGL+K+ G     T      Q+RG+PLYM
Sbjct: 121 LRHIHANGFAHCDLKLGNILLFGDGAVKIADFGLAKRIGD---LTALNYGVQIRGTPLYM 180

Query: 181 SPESVNDDEYESPCDIWAVGCAVVEMLTGKPAWNFPVECNIYSLLLKIGIGEELPKIPKY 240
           +PESVND+EY S  D+WA+GC VVEM +GK AW+     N  SLLL+IG+G+E+P IP+ 
Sbjct: 181 APESVNDNEYGSEGDVWALGCVVVEMFSGKTAWSLKEGSNFMSLLLRIGVGDEVPMIPEE 240

Query: 241 ISEEGKDFLGKCLVKDPAKRWTAEMLLNHPFLADSGSSSVPLTPKG--------EPLTSP 300
           +SE+G+DFL KC VKDP KRWTAEMLLNHPF+       V +  +         +  TSP
Sbjct: 241 LSEQGRDFLSKCFVKDPKKRWTAEMLLNHPFVTVDVDHDVLVKEEDFVVNMKTEDVSTSP 300

Query: 301 RCPFDFEDWASIHSLEMDQKFEEESNCWLDSSCPWSCSPTERLLQLVDNGPVDWVITDSW 355
           RCPF+F DW S+ S           +  +D       SP ER+  LV +   DW +T+SW
Sbjct: 301 RCPFEFPDWVSVSS----------GSQTID-------SPDERVASLVTDMIPDWSVTNSW 344

BLAST of CmaCh07G007010 vs. TAIR 10
Match: AT5G55090.1 (mitogen-activated protein kinase kinase kinase 15 )

HSP 1 Score: 234.6 bits (597), Expect = 1.2e-61
Identity = 139/360 (38.61%), Postives = 197/360 (54.72%), Query Frame = 0

Query: 3   WIRGHEIGCGSFATINLAMPSNTSNAQFPPLMAVKSSPAVSSASLKNEKRVLDLLGDCPQ 62
           WIRG  IG GS AT++L +   T++  F    AVKS+   SSA L+ E+ +L  L   P 
Sbjct: 6   WIRGPIIGRGSTATVSLGI---TNSGDF---FAVKSAEFSSSAFLQREQSILSKLSS-PY 65

Query: 63  IIRCFGESCSVERGDELYNLFLEYASGGSLADRIQSHGGWLPEFEVRRFTRTILEGLRYI 122
           I++  G + + E    +YNL +EY SGGSL D I++ GG LPE  +R +TR IL+GL Y+
Sbjct: 66  IVKYIGSNVTKENDKLMYNLLMEYVSGGSLHDLIKNSGGKLPEPLIRSYTRQILKGLMYL 125

Query: 123 HEKGFVHCDVKPRNILVFGDGDAKIADFGLSKKAGKNRAETGEEGKFQLRGSPLYMSPES 182
           H++G VHCDVK +N+++ G+  AKI D G +K   +N          +  G+P +MSPE 
Sbjct: 126 HDQGIVHCDVKSQNVMIGGE-IAKIVDLGCAKTVEENE-------NLEFSGTPAFMSPEV 185

Query: 183 VNDDEYESPCDIWAVGCAVVEMLTGKPAWNFPVECNIYSLLLKIGIGEELPKIPKYISEE 242
              +E   P D+WA+GC V+EM TG   W  P   ++ + + KIG   E P IP ++SE+
Sbjct: 186 ARGEEQSFPADVWALGCTVIEMATGSSPW--PELNDVVAAIYKIGFTGESPVIPVWLSEK 245

Query: 243 GKDFLGKCLVKDPAKRWTAEMLLNHPFLADSGSSSVPLTPKGEPLTSPRCPFDFEDW--- 302
           G+DFL KCL KDP +RWT E LL HPFL +  + S   T      +SP    D   W   
Sbjct: 246 GQDFLRKCLRKDPKQRWTVEELLQHPFLDEEDNDS-DQTGNCLNSSSPSTVLDQRFWDLC 305

Query: 303 ----ASIHSLEMDQKFEEESN-CWLDSSCPWSCSPTERLLQLVDNGPVDWVITDSWVTVR 355
               +     + +  F   +N  W D S P      ++L     +G  DW  T+ W+ VR
Sbjct: 306 ETSRSRFIKEDHEDPFANSTNFLWDDDSLPG--DRIKKLAGDESSGEPDWE-TNGWIEVR 344

BLAST of CmaCh07G007010 vs. TAIR 10
Match: AT4G26890.1 (mitogen-activated protein kinase kinase kinase 16 )

HSP 1 Score: 232.6 bits (592), Expect = 4.7e-61
Identity = 136/366 (37.16%), Postives = 197/366 (53.83%), Query Frame = 0

Query: 1   MEWIRGHEIGCGSFATINLAMPSNTSNAQFPPLMAVKSSPAVSSASLKNEKRVLDLLGDC 60
           + W RG  IG GS AT+++A+ S+        L AVKS+   SS+ L+ E+ +L  L   
Sbjct: 3   INWTRGPIIGRGSTATVSIAISSSGE------LFAVKSADLSSSSLLQKEQSILSTLSS- 62

Query: 61  PQIIRCFGESCSVERGDELYNLFLEYASGGSLADRIQSHGGWLPEFEVRRFTRTILEGLR 120
           P +++  G   + E    +YN+ +EY SGG+L D I++ GG LPE E+R +TR IL GL 
Sbjct: 63  PHMVKYIGTGLTRESNGLVYNILMEYVSGGNLHDLIKNSGGKLPEPEIRSYTRQILNGLV 122

Query: 121 YIHEKGFVHCDVKPRNILVFGDGDAKIADFGLSKKAGKNRAETGEEGKFQLRGSPLYMSP 180
           Y+HE+G VHCD+K  N+LV  +G  KIAD G +K   K+          +  G+P +M+P
Sbjct: 123 YLHERGIVHCDLKSHNVLVEENGVLKIADMGCAKSVDKS----------EFSGTPAFMAP 182

Query: 181 ESVNDDEYESPCDIWAVGCAVVEMLTGKPAWNFPVECNIYSLLLKIGIGEELPKIPKYIS 240
           E    +E   P D+WA+GC ++EM+TG   W  P   ++ + + KIG   E P IP +IS
Sbjct: 183 EVARGEEQRFPADVWALGCTMIEMMTGSSPW--PELNDVVAAMYKIGFSGESPAIPAWIS 242

Query: 241 EEGKDFLGKCLVKDPAKRWTAEMLLNHPFLADSGSSSVPLTPKGEPLTSPRCPFDFEDWA 300
           ++ KDFL  CL +D  +RWT E LL HPFL D   S      K +  +SP    D   W 
Sbjct: 243 DKAKDFLKNCLKEDQKQRWTVEELLKHPFLDDDEESQTSDCLKNK-TSSPSTVLDQRFWD 302

Query: 301 S-------IHSLEMDQKFEEESNCWLDSSCPWSCSPTERLLQLVD---NGPVDWVITD-- 355
           S       + S++ +  F E S   LD       SP +R+ +L     +  +DW   D  
Sbjct: 303 SCESSKSHLVSIDHEDPFAEYSES-LD-------SPADRIEKLAGDEFSSLLDWDTEDDG 340

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SND61.3e-10052.91Mitogen-activated protein kinase kinase kinase 20 OS=Arabidopsis thaliana OX=370... [more]
O808884.5e-5637.12Mitogen-activated protein kinase kinase kinase 17 OS=Arabidopsis thaliana OX=370... [more]
Q9ZVP56.1e-5336.24Mitogen-activated protein kinase kinase kinase 18 OS=Arabidopsis thaliana OX=370... [more]
O220421.7e-4234.11Mitogen-activated protein kinase kinase kinase 3 OS=Arabidopsis thaliana OX=3702... [more]
Q405411.7e-4235.52Mitogen-activated protein kinase kinase kinase NPK1 OS=Nicotiana tabacum OX=4097... [more]
Match NameE-valueIdentityDescription
A0A6J1KQ575.4e-214100.00mitogen-activated protein kinase kinase kinase 18-like OS=Cucurbita maxima OX=36... [more]
A0A6J1HG324.9e-20796.89mitogen-activated protein kinase kinase kinase 17-like OS=Cucurbita moschata OX=... [more]
A0A5D3CM361.5e-16380.11Mitogen-activated protein kinase kinase kinase 3-like OS=Cucumis melo var. makuw... [more]
A0A1S3B1I01.5e-16380.11mitogen-activated protein kinase kinase kinase 3-like OS=Cucumis melo OX=3656 GN... [more]
A0A0A0KLC92.6e-16079.21Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G490... [more]
Match NameE-valueIdentityDescription
XP_023003761.11.1e-213100.00mitogen-activated protein kinase kinase kinase 18-like [Cucurbita maxima][more]
XP_023517683.13.5e-20797.18mitogen-activated protein kinase kinase kinase 17-like [Cucurbita pepo subsp. pe... [more]
KAG6595031.14.6e-20796.89Mitogen-activated protein kinase kinase kinase 20, partial [Cucurbita argyrosper... [more]
XP_022962795.11.0e-20696.89mitogen-activated protein kinase kinase kinase 17-like [Cucurbita moschata][more]
KAG7027055.13.9e-20696.61Mitogen-activated protein kinase kinase kinase 17, partial [Cucurbita argyrosper... [more]
Match NameE-valueIdentityDescription
AT4G36950.11.0e-10354.32mitogen-activated protein kinase kinase kinase 21 [more]
AT3G50310.19.5e-10252.91mitogen-activated protein kinase kinase kinase 20 [more]
AT5G67080.19.5e-10251.92mitogen-activated protein kinase kinase kinase 19 [more]
AT5G55090.11.2e-6138.61mitogen-activated protein kinase kinase kinase 15 [more]
AT4G26890.14.7e-6137.16mitogen-activated protein kinase kinase kinase 16 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000719Protein kinase domainSMARTSM00220serkin_6coord: 3..270
e-value: 3.7E-68
score: 242.4
IPR000719Protein kinase domainPFAMPF00069Pkinasecoord: 6..270
e-value: 1.4E-53
score: 182.0
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 3..270
score: 39.240944
NoneNo IPR availableGENE3D3.30.200.20Phosphorylase Kinase; domain 1coord: 1..93
e-value: 2.6E-8
score: 35.9
NoneNo IPR availableGENE3D1.10.510.10Transferase(Phosphotransferase) domain 1coord: 94..286
e-value: 1.5E-53
score: 183.6
NoneNo IPR availablePIRSRPIRSR038172-1PIRSR038172-1coord: 10..275
e-value: 3.2E-26
score: 89.8
NoneNo IPR availablePIRSRPIRSR630220-2PIRSR630220-2coord: 83..260
e-value: 1.0E-12
score: 44.9
NoneNo IPR availablePIRSRPIRSR037993-2PIRSR037993-2coord: 92..275
e-value: 4.8E-12
score: 43.2
NoneNo IPR availablePANTHERPTHR48011CCR4-NOT TRANSCRIPTIONAL COMPLEX SUBUNIT CAF120-RELATEDcoord: 1..312
NoneNo IPR availablePANTHERPTHR48011:SF18MITOGEN-ACTIVATED PROTEIN KINASE KINASE KINASE 20coord: 1..312
NoneNo IPR availableCDDcd06606STKc_MAPKKKcoord: 2..270
e-value: 2.53489E-117
score: 338.341
IPR008271Serine/threonine-protein kinase, active sitePROSITEPS00108PROTEIN_KINASE_STcoord: 127..139
IPR017441Protein kinase, ATP binding sitePROSITEPS00107PROTEIN_KINASE_ATPcoord: 9..37
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 3..292

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh07G007010.1CmaCh07G007010.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006468 protein phosphorylation
molecular_function GO:0005524 ATP binding
molecular_function GO:0004674 protein serine/threonine kinase activity
molecular_function GO:0004672 protein kinase activity