CmaCh07G004580 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh07G004580
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
DescriptionrRNA adenine N(6)-methyltransferase
LocationCma_Chr07: 1999057 .. 2000716 (+)
RNA-Seq ExpressionCmaCh07G004580
SyntenyCmaCh07G004580
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GCAGTGTCTCCATTGGAATCAACTGAGCTTCGAGAATTTATGCAAATCTTCGCGTTGCTGCTTCTGATTCCTATCGAATAGTGAAATCCCTAATGCTTCGTCCCCATAAAAATCTATGCAGGTTCATCAACGCCCTGAACCACGACCAATTCCTTCACTGTACACGCTCGGCATCCTCTTATCAATACAACCACAGAAACCATGGCGAGAAAGAAAACGTTCCCAAGGATGGGAACGAAGAACCTCAATTGTATTTCTACAAGAGTAGAGGCCAGCACATTCTTACGAACCCACGAATTCTGGGCTCTATTGTTCGAAAATCTGCCATAAGGCCGACCGACACGGTCTTAGAGATCGGACCCGGCACCGGAAATCTGACATTGAAGCTTCTGGAAGCTTCCCAAAAGGTCATAGCCATTGAAATTGATAAAAGAATGGTCGAGGTTCTGCACAGACGCGTGGCGGAGAATAAGTTTGAAGACCGTATTTGTGTAAGTTAATATTGCTGTCTATGTGTTCGATGAAATGTCTGACAGAGGATAAACTGCTTTGAAATGTGGGAGTGGGGGTTTTTTTGTAGGTTATACGCGAAGATGCTTTGAAATGTGAGTTCCCTCCTTTCGACCTCGTCGTGGCCAACATCCCTTATGGGATATCTTCGCCGCTTGTGGCCAAATTGGTATATGGGGCAATTCCATTTCGGAGTGCCACGCTTCTACTTCAGAAAGAGTTTGCACGGCGGTTGTTGGCGAATCCTGGTGACTCTGAGTACAATCGTTTGGCTGTAAATGTGAAATTGGTGGCTGATGTAGAGCATGTAATGGATGTAAGCAAGAGGGACTTTGTTCCTTGTCCTAAAGTCGACTCATCCGTGGTGATAATCCGCCCCAAGAAGGAAGTTCCTGAAGTGAACCTGGAAGAATGGCGGGCTTTCACTAGAACTTGCTTTGGGAAGAAGAACAAGACACTTGGGGCAACGTTCAAGCAGAAGAGGATGCTGATGGCCCTAATGAACTCCAATTCTGAGAGTTTCAATGGTCGAGGAGTTGAAGAAGAGGGCTGTTCTTCATCTTCAGAGAAGGGGTTTAATTTGTTCAAGGAGAAAATCATAGAGGTTTTGAAATCTGGGAAGTTTGAAGATAAGAGGCCTGCAAAGTTGTGTAACTTGAAGTTGCTGCATTTGCTGGCGTTGTTCAATCAAGCTGGGATATATTTTCGTGATGGATCAGAGCTTCAGGATGCAGAGAGAAGCGCCTTAGCTGATGATGCCTACGGATCAGAACTAGAAGATTAGCTGATCGTTAAATTTTGTATTCATTTGAGAGAGAAACACCATTCTTCCAGTTGAAATGTCACTGCAGTTTTTTATCTTGCTTCTTGAAATATAATTTGCAGTCTTCTTCGAAAAGAAACTGAACCCTTCTTTGTGTTGATTAGAGGTACTGACGACAGAGTTCAACTCTTTGAGCGATTTCCATCCATTTGGGTTGGTTTATTCTATAAACTTAAGTTACAAAAGGGAGCTATTACAATGATACCATTGAAGATAGAGTGTCACGACCATGCTTGTATGAAACGTATTGCCTGTGGCTGTATGCTTAATATCACGGTTATGCTTATATAGGGTAGAGTGTGTTGTTTGTGGCCGTACGCTTATTTC

mRNA sequence

GCAGTGTCTCCATTGGAATCAACTGAGCTTCGAGAATTTATGCAAATCTTCGCGTTGCTGCTTCTGATTCCTATCGAATAGTGAAATCCCTAATGCTTCGTCCCCATAAAAATCTATGCAGGTTCATCAACGCCCTGAACCACGACCAATTCCTTCACTGTACACGCTCGGCATCCTCTTATCAATACAACCACAGAAACCATGGCGAGAAAGAAAACGTTCCCAAGGATGGGAACGAAGAACCTCAATTGTATTTCTACAAGAGTAGAGGCCAGCACATTCTTACGAACCCACGAATTCTGGGCTCTATTGTTCGAAAATCTGCCATAAGGCCGACCGACACGGTCTTAGAGATCGGACCCGGCACCGGAAATCTGACATTGAAGCTTCTGGAAGCTTCCCAAAAGGTCATAGCCATTGAAATTGATAAAAGAATGGTCGAGGTTCTGCACAGACGCGTGGCGGAGAATAAGTTTGAAGACCGTATTTGTGTTATACGCGAAGATGCTTTGAAATGTGAGTTCCCTCCTTTCGACCTCGTCGTGGCCAACATCCCTTATGGGATATCTTCGCCGCTTGTGGCCAAATTGGTATATGGGGCAATTCCATTTCGGAGTGCCACGCTTCTACTTCAGAAAGAGTTTGCACGGCGGTTGTTGGCGAATCCTGGTGACTCTGAGTACAATCGTTTGGCTGTAAATGTGAAATTGGTGGCTGATGTAGAGCATGTAATGGATGTAAGCAAGAGGGACTTTGTTCCTTGTCCTAAAGTCGACTCATCCGTGGTGATAATCCGCCCCAAGAAGGAAGTTCCTGAAGTGAACCTGGAAGAATGGCGGGCTTTCACTAGAACTTGCTTTGGGAAGAAGAACAAGACACTTGGGGCAACGTTCAAGCAGAAGAGGATGCTGATGGCCCTAATGAACTCCAATTCTGAGAGTTTCAATGGTCGAGGAGTTGAAGAAGAGGGCTGTTCTTCATCTTCAGAGAAGGGGTTTAATTTGTTCAAGGAGAAAATCATAGAGGTTTTGAAATCTGGGAAGTTTGAAGATAAGAGGCCTGCAAAGTTGTGTAACTTGAAGTTGCTGCATTTGCTGGCGTTGTTCAATCAAGCTGGGATATATTTTCGTGATGGATCAGAGCTTCAGGATGCAGAGAGAAGCGCCTTAGCTGATGATGCCTACGGATCAGAACTAGAAGATTAGCTGATCGTTAAATTTTGTATTCATTTGAGAGAGAAACACCATTCTTCCAGTTGAAATGTCACTGCAGTTTTTTATCTTGCTTCTTGAAATATAATTTGCAGTCTTCTTCGAAAAGAAACTGAACCCTTCTTTGTGTTGATTAGAGGTACTGACGACAGAGTTCAACTCTTTGAGCGATTTCCATCCATTTGGGTTGGTTTATTCTATAAACTTAAGTTACAAAAGGGAGCTATTACAATGATACCATTGAAGATAGAGTGTCACGACCATGCTTGTATGAAACGTATTGCCTGTGGCTGTATGCTTAATATCACGGTTATGCTTATATAGGGTAGAGTGTGTTGTTTGTGGCCGTACGCTTATTTC

Coding sequence (CDS)

ATGCTTCGTCCCCATAAAAATCTATGCAGGTTCATCAACGCCCTGAACCACGACCAATTCCTTCACTGTACACGCTCGGCATCCTCTTATCAATACAACCACAGAAACCATGGCGAGAAAGAAAACGTTCCCAAGGATGGGAACGAAGAACCTCAATTGTATTTCTACAAGAGTAGAGGCCAGCACATTCTTACGAACCCACGAATTCTGGGCTCTATTGTTCGAAAATCTGCCATAAGGCCGACCGACACGGTCTTAGAGATCGGACCCGGCACCGGAAATCTGACATTGAAGCTTCTGGAAGCTTCCCAAAAGGTCATAGCCATTGAAATTGATAAAAGAATGGTCGAGGTTCTGCACAGACGCGTGGCGGAGAATAAGTTTGAAGACCGTATTTGTGTTATACGCGAAGATGCTTTGAAATGTGAGTTCCCTCCTTTCGACCTCGTCGTGGCCAACATCCCTTATGGGATATCTTCGCCGCTTGTGGCCAAATTGGTATATGGGGCAATTCCATTTCGGAGTGCCACGCTTCTACTTCAGAAAGAGTTTGCACGGCGGTTGTTGGCGAATCCTGGTGACTCTGAGTACAATCGTTTGGCTGTAAATGTGAAATTGGTGGCTGATGTAGAGCATGTAATGGATGTAAGCAAGAGGGACTTTGTTCCTTGTCCTAAAGTCGACTCATCCGTGGTGATAATCCGCCCCAAGAAGGAAGTTCCTGAAGTGAACCTGGAAGAATGGCGGGCTTTCACTAGAACTTGCTTTGGGAAGAAGAACAAGACACTTGGGGCAACGTTCAAGCAGAAGAGGATGCTGATGGCCCTAATGAACTCCAATTCTGAGAGTTTCAATGGTCGAGGAGTTGAAGAAGAGGGCTGTTCTTCATCTTCAGAGAAGGGGTTTAATTTGTTCAAGGAGAAAATCATAGAGGTTTTGAAATCTGGGAAGTTTGAAGATAAGAGGCCTGCAAAGTTGTGTAACTTGAAGTTGCTGCATTTGCTGGCGTTGTTCAATCAAGCTGGGATATATTTTCGTGATGGATCAGAGCTTCAGGATGCAGAGAGAAGCGCCTTAGCTGATGATGCCTACGGATCAGAACTAGAAGATTAG

Protein sequence

MLRPHKNLCRFINALNHDQFLHCTRSASSYQYNHRNHGEKENVPKDGNEEPQLYFYKSRGQHILTNPRILGSIVRKSAIRPTDTVLEIGPGTGNLTLKLLEASQKVIAIEIDKRMVEVLHRRVAENKFEDRICVIREDALKCEFPPFDLVVANIPYGISSPLVAKLVYGAIPFRSATLLLQKEFARRLLANPGDSEYNRLAVNVKLVADVEHVMDVSKRDFVPCPKVDSSVVIIRPKKEVPEVNLEEWRAFTRTCFGKKNKTLGATFKQKRMLMALMNSNSESFNGRGVEEEGCSSSSEKGFNLFKEKIIEVLKSGKFEDKRPAKLCNLKLLHLLALFNQAGIYFRDGSELQDAERSALADDAYGSELED
Homology
BLAST of CmaCh07G004580 vs. ExPASy Swiss-Prot
Match: Q9FK02 (Ribosomal RNA small subunit methyltransferase, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=DIM1B PE=1 SV=1)

HSP 1 Score: 386.7 bits (992), Expect = 2.9e-106
Identity = 205/337 (60.83%), Postives = 263/337 (78.04%), Query Frame = 0

Query: 32  YNHRNHGEKENVP-KDGNEEPQLYFYKSRGQHILTNPRILGSIVRKSAIRPTDTVLEIGP 91
           ++ R  G + +V  ++  E   L+  KS+GQH+LTN RIL SIVR S IRPTDTVLEIGP
Sbjct: 39  HDRRRRGYERDVRIEEKKEHDGLFLCKSKGQHLLTNTRILDSIVRSSDIRPTDTVLEIGP 98

Query: 92  GTGNLTLKLLEASQKVIAIEIDKRMVEVLHRRVAENKFEDRICVIREDALKCEFPPFDLV 151
           GTGNLT+KLLEA+Q V+A+E+DKRMVE+L +RV+++ F D++ +I++D LK +FP FDLV
Sbjct: 99  GTGNLTMKLLEAAQNVVAVELDKRMVEILRKRVSDHGFADKLTIIQKDVLKTDFPHFDLV 158

Query: 152 VANIPYGISSPLVAKLVYGAIPFRSATLLLQKEFARRLLANPGDSEYNRLAVNVKLVADV 211
           VANIPY ISSPLVAKLVYG+  FRSATLLLQKEF+RRLLANPGDS++NRLAVNVKLVADV
Sbjct: 159 VANIPYNISSPLVAKLVYGSNTFRSATLLLQKEFSRRLLANPGDSDFNRLAVNVKLVADV 218

Query: 212 EHVMDVSKRDFVPCPKVDSSVVIIRPKKEVPEVNLEEWRAFTRTCFGKKNKTLGATFKQK 271
           + VMDVSKR+FVP PKVDSSV+ I PK+ +P+VN++EW AFTRTCFGKKNKTLG+ F+QK
Sbjct: 219 KFVMDVSKREFVPPPKVDSSVIRITPKEIIPDVNVQEWLAFTRTCFGKKNKTLGSMFRQK 278

Query: 272 RMLMALMN-------SNSESFNGRG------VEEEGCSS--SSEKGFNLFKEKIIEVLKS 331
           + +M L +       SN E  N  G      VEE+G       +   ++FKE++IE+L++
Sbjct: 279 KKVMELQSLSAGRHGSNVEVMNQTGGDSDSDVEEDGKDDLLCLDTDASMFKERVIEILRT 338

Query: 332 GKFEDKRPAKLCNLKLLHLLALFNQAGIYFRDGSELQ 353
             FE+KRP+KL + +LLHLL+LFNQAGI+F D + LQ
Sbjct: 339 NGFEEKRPSKLSHRELLHLLSLFNQAGIFFHDITSLQ 375

BLAST of CmaCh07G004580 vs. ExPASy Swiss-Prot
Match: O22268 (Ribosomal RNA small subunit methyltransferase OS=Arabidopsis thaliana OX=3702 GN=DIM1A PE=1 SV=1)

HSP 1 Score: 273.9 bits (699), Expect = 2.7e-72
Identity = 159/348 (45.69%), Postives = 213/348 (61.21%), Query Frame = 0

Query: 40  KENVPKDGNEEPQ------LYFYKSRGQHILTNPRILGSIVRKSAIRPTDTVLEIGPGTG 99
           ++  PK  N  P       + F+KS+GQHIL NP ++ SIV+K+ I+ TD +LEIGPGTG
Sbjct: 7   RKEKPKASNRAPSNHYQGGISFHKSKGQHILKNPLLVDSIVQKAGIKSTDVILEIGPGTG 66

Query: 100 NLTLKLLEASQKVIAIEIDKRMVEVLHRRVAENKFEDRICVIREDALKCEFPPFDLVVAN 159
           NLT KLLEA ++VIA+E+D RMV  L RR     F +R+ VI+ D LK E P FD+ VAN
Sbjct: 67  NLTKKLLEAGKEVIAVELDSRMVLELQRRFQGTPFSNRLKVIQGDVLKTELPRFDICVAN 126

Query: 160 IPYGISSPLVAKLVYGAIPFRSATLLLQKEFARRLLANPGDSEYNRLAVNVKLVADVEHV 219
           IPY ISSPL  KL++    FR A ++ Q+EFA RL+A PGD+ Y RL+VN +L A V H+
Sbjct: 127 IPYQISSPLTFKLLFHPTSFRCAVIMYQREFAMRLVAQPGDNLYCRLSVNTQLYARVSHL 186

Query: 220 MDVSKRDFVPCPKVDSSVVIIRPKKEVPEVNLEEWRAFTRTCFGKKNKTLGATFKQK--- 279
           + V K +F P PKVDSSVV I P++  P+VN +EW  F R CF +KNKTLG+ FKQK   
Sbjct: 187 LKVGKNNFRPPPKVDSSVVRIEPRRPGPQVNKKEWDGFLRVCFIRKNKTLGSIFKQKSVL 246

Query: 280 -----------RMLMALMNSNSESFN---------GRGVE-------------EEGCSSS 339
                       +L +L N+   + N           G+E             +EG    
Sbjct: 247 SMLEKNFKTLQAVLASLQNNGEPALNTTSMDLGDQSMGMEDDDNEMDDDDMEMDEGEGDG 306

Query: 340 SEKGFNLFKEKIIEVLKSGKFEDKRPAKLCNLKLLHLLALFNQAGIYF 346
            E   + FKEK++ VLK G FE+KR +KL   + L+LL+LFN++GI+F
Sbjct: 307 GET--SEFKEKVMNVLKEGGFEEKRSSKLSQQEFLYLLSLFNKSGIHF 352

BLAST of CmaCh07G004580 vs. ExPASy Swiss-Prot
Match: Q54QK7 (Probable dimethyladenosine transferase OS=Dictyostelium discoideum OX=44689 GN=dimt1 PE=3 SV=1)

HSP 1 Score: 233.8 bits (595), Expect = 3.1e-60
Identity = 135/290 (46.55%), Postives = 181/290 (62.41%), Query Frame = 0

Query: 57  KSRGQHILTNPRILGSIVRKSAIRPTDTVLEIGPGTGNLTLKLLEASQKVIAIEIDKRMV 116
           KS GQH+L NP I+ +IV KS ++ TDTVLEIGPGTGNLT+KLLE  +KVIAIE+D RM 
Sbjct: 31  KSYGQHLLKNPLIIDAIVDKSQLKSTDTVLEIGPGTGNLTMKLLENCKKVIAIEVDPRMA 90

Query: 117 EVLHRRVAENKFEDRICVIREDALKCEFPPFDLVVANIPYGISSPLVAKLVYGAIPFRSA 176
             L +RVA + +   + +I  D LK + P FD+ VAN+PY ISSPL  KL+     FR+A
Sbjct: 91  AELQKRVAASPYAQHLQIILGDFLKVDLPYFDVCVANVPYQISSPLTFKLLAHRPIFRTA 150

Query: 177 TLLLQKEFARRLLANPGDSEYNRLAVNVKLVADVEHVMDVSKRDFVPCPKVDSSVVIIRP 236
            L+ QKEFA RL A PGDS Y RL+VN +L++ V H+M V K +F+P PKV+S+VV I P
Sbjct: 151 VLMFQKEFALRLGAKPGDSLYCRLSVNTQLLSKVTHLMKVGKNNFLPPPKVESAVVRIEP 210

Query: 237 KKEVPEVNLEEWRAFTRTCFGKKNKTLGATFKQKRMLMALMNSNSESFNGRGVEEEGCSS 296
               P +N  EW    + CF +KNKTL   F+   ++  L N N +++       EG   
Sbjct: 211 FNPPPPINFVEWDGLVKLCFSRKNKTLSGIFRVSSVIETL-NQNYKTY----CALEG-KM 270

Query: 297 SSEKGFNLFKEKIIEVLKSGKFEDKRPAKLCNLKLLHLLALFNQAGIYFR 347
           +++      KE II+ L    F D R +KL     L LL  F++ GI+F+
Sbjct: 271 NTDGSDEQMKELIIKTLTDNDFLDSRSSKLDINDFLKLLNKFHETGIHFK 314

BLAST of CmaCh07G004580 vs. ExPASy Swiss-Prot
Match: Q2KHT8 (Probable dimethyladenosine transferase OS=Bos taurus OX=9913 GN=DIMT1 PE=2 SV=1)

HSP 1 Score: 228.4 bits (581), Expect = 1.3e-58
Identity = 134/296 (45.27%), Postives = 178/296 (60.14%), Query Frame = 0

Query: 53  LYFYKSRGQHILTNPRILGSIVRKSAIRPTDTVLEIGPGTGNLTLKLLEASQKVIAIEID 112
           L F    GQHIL NP I+ SI+ K+A+RPTD VLE+GPGTGN+T+KLLE ++KVIA E+D
Sbjct: 28  LMFNTGIGQHILKNPLIVNSIIDKAALRPTDVVLEVGPGTGNMTVKLLEKAKKVIACELD 87

Query: 113 KRMVEVLHRRVAENKFEDRICVIREDALKCEFPPFDLVVANIPYGISSPLVAKLVYGAIP 172
            R+V  LH+RV       ++ V+  D LK + P FD  VAN+PY ISSP V KL+     
Sbjct: 88  PRLVAELHKRVQGTPLASKLQVMVGDVLKADLPFFDACVANLPYQISSPFVFKLLLHRPF 147

Query: 173 FRSATLLLQKEFARRLLANPGDSEYNRLAVNVKLVADVEHVMDVSKRDFVPCPKVDSSVV 232
           FR A L+ Q+EFA RL+A PGD  Y RL++N +L+A V+H+M V K +F P PKV+SSVV
Sbjct: 148 FRCAVLMFQREFALRLVAKPGDKLYCRLSINTQLLARVDHLMKVGKNNFRPPPKVESSVV 207

Query: 233 IIRPKKEVPEVNLEEWRAFTRTCFGKKNKTLGATFKQKRMLMALMNSNSESFNGRGVEEE 292
            I PK   P +N +EW    R  F +KNKTL A FK    +  L+  N            
Sbjct: 208 RIEPKNPPPPINFQEWDGLVRITFVRKNKTLSAAFKSS-AVQQLLEKNYRIH-------- 267

Query: 293 GCSSSS---EKGFNLFKEKIIEVLKSGKFEDKRPAKLCNLKLLHLLALFNQAGIYF 346
            CS  +    + F++  +KI ++L S  F DKR   +     + LL  FN  GI+F
Sbjct: 268 -CSVHNIIIPEDFSI-ADKIQQILTSTGFSDKRARSMDIDDFIRLLHGFNAEGIHF 312

BLAST of CmaCh07G004580 vs. ExPASy Swiss-Prot
Match: Q9UNQ2 (Probable dimethyladenosine transferase OS=Homo sapiens OX=9606 GN=DIMT1 PE=1 SV=1)

HSP 1 Score: 227.6 bits (579), Expect = 2.2e-58
Identity = 133/296 (44.93%), Postives = 178/296 (60.14%), Query Frame = 0

Query: 53  LYFYKSRGQHILTNPRILGSIVRKSAIRPTDTVLEIGPGTGNLTLKLLEASQKVIAIEID 112
           L F    GQHIL NP I+ SI+ K+A+RPTD VLE+GPGTGN+T+KLLE ++KV+A E+D
Sbjct: 28  LMFNTGIGQHILKNPLIINSIIDKAALRPTDVVLEVGPGTGNMTVKLLEKAKKVVACELD 87

Query: 113 KRMVEVLHRRVAENKFEDRICVIREDALKCEFPPFDLVVANIPYGISSPLVAKLVYGAIP 172
            R+V  LH+RV       ++ V+  D LK + P FD  VAN+PY ISSP V KL+     
Sbjct: 88  PRLVAELHKRVQGTPVASKLQVLVGDVLKTDLPFFDTCVANLPYQISSPFVFKLLLHRPF 147

Query: 173 FRSATLLLQKEFARRLLANPGDSEYNRLAVNVKLVADVEHVMDVSKRDFVPCPKVDSSVV 232
           FR A L+ Q+EFA RL+A PGD  Y RL++N +L+A V+H+M V K +F P PKV+SSVV
Sbjct: 148 FRCAILMFQREFALRLVAKPGDKLYCRLSINTQLLARVDHLMKVGKNNFRPPPKVESSVV 207

Query: 233 IIRPKKEVPEVNLEEWRAFTRTCFGKKNKTLGATFKQKRMLMALMNSNSESFNGRGVEEE 292
            I PK   P +N +EW    R  F +KNKTL A FK    +  L+  N            
Sbjct: 208 RIEPKNPPPPINFQEWDGLVRITFVRKNKTLSAAFKSS-AVQQLLEKNYRIH-------- 267

Query: 293 GCSSSS---EKGFNLFKEKIIEVLKSGKFEDKRPAKLCNLKLLHLLALFNQAGIYF 346
            CS  +    + F++  +KI ++L S  F DKR   +     + LL  FN  GI+F
Sbjct: 268 -CSVHNIIIPEDFSI-ADKIQQILTSTGFSDKRARSMDIDDFIRLLHGFNAEGIHF 312

BLAST of CmaCh07G004580 vs. ExPASy TrEMBL
Match: A0A6J1KLE6 (rRNA adenine N(6)-methyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111496791 PE=3 SV=1)

HSP 1 Score: 732.3 bits (1889), Expect = 1.0e-207
Identity = 370/370 (100.00%), Postives = 370/370 (100.00%), Query Frame = 0

Query: 1   MLRPHKNLCRFINALNHDQFLHCTRSASSYQYNHRNHGEKENVPKDGNEEPQLYFYKSRG 60
           MLRPHKNLCRFINALNHDQFLHCTRSASSYQYNHRNHGEKENVPKDGNEEPQLYFYKSRG
Sbjct: 1   MLRPHKNLCRFINALNHDQFLHCTRSASSYQYNHRNHGEKENVPKDGNEEPQLYFYKSRG 60

Query: 61  QHILTNPRILGSIVRKSAIRPTDTVLEIGPGTGNLTLKLLEASQKVIAIEIDKRMVEVLH 120
           QHILTNPRILGSIVRKSAIRPTDTVLEIGPGTGNLTLKLLEASQKVIAIEIDKRMVEVLH
Sbjct: 61  QHILTNPRILGSIVRKSAIRPTDTVLEIGPGTGNLTLKLLEASQKVIAIEIDKRMVEVLH 120

Query: 121 RRVAENKFEDRICVIREDALKCEFPPFDLVVANIPYGISSPLVAKLVYGAIPFRSATLLL 180
           RRVAENKFEDRICVIREDALKCEFPPFDLVVANIPYGISSPLVAKLVYGAIPFRSATLLL
Sbjct: 121 RRVAENKFEDRICVIREDALKCEFPPFDLVVANIPYGISSPLVAKLVYGAIPFRSATLLL 180

Query: 181 QKEFARRLLANPGDSEYNRLAVNVKLVADVEHVMDVSKRDFVPCPKVDSSVVIIRPKKEV 240
           QKEFARRLLANPGDSEYNRLAVNVKLVADVEHVMDVSKRDFVPCPKVDSSVVIIRPKKEV
Sbjct: 181 QKEFARRLLANPGDSEYNRLAVNVKLVADVEHVMDVSKRDFVPCPKVDSSVVIIRPKKEV 240

Query: 241 PEVNLEEWRAFTRTCFGKKNKTLGATFKQKRMLMALMNSNSESFNGRGVEEEGCSSSSEK 300
           PEVNLEEWRAFTRTCFGKKNKTLGATFKQKRMLMALMNSNSESFNGRGVEEEGCSSSSEK
Sbjct: 241 PEVNLEEWRAFTRTCFGKKNKTLGATFKQKRMLMALMNSNSESFNGRGVEEEGCSSSSEK 300

Query: 301 GFNLFKEKIIEVLKSGKFEDKRPAKLCNLKLLHLLALFNQAGIYFRDGSELQDAERSALA 360
           GFNLFKEKIIEVLKSGKFEDKRPAKLCNLKLLHLLALFNQAGIYFRDGSELQDAERSALA
Sbjct: 301 GFNLFKEKIIEVLKSGKFEDKRPAKLCNLKLLHLLALFNQAGIYFRDGSELQDAERSALA 360

Query: 361 DDAYGSELED 371
           DDAYGSELED
Sbjct: 361 DDAYGSELED 370

BLAST of CmaCh07G004580 vs. ExPASy TrEMBL
Match: A0A6J1HJI5 (rRNA adenine N(6)-methyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111463514 PE=3 SV=1)

HSP 1 Score: 699.9 bits (1805), Expect = 5.7e-198
Identity = 356/371 (95.96%), Postives = 361/371 (97.30%), Query Frame = 0

Query: 1   MLRPHKNLCRFINALNHDQFLHCTRSASSYQYNHRNHGEKENVP-KDGNEEPQLYFYKSR 60
           MLRPHKNLCRFIN LNHDQFLHC RSASSYQYNHRNHG KENVP KDGNEEPQLYFYKSR
Sbjct: 1   MLRPHKNLCRFINGLNHDQFLHCKRSASSYQYNHRNHGGKENVPKKDGNEEPQLYFYKSR 60

Query: 61  GQHILTNPRILGSIVRKSAIRPTDTVLEIGPGTGNLTLKLLEASQKVIAIEIDKRMVEVL 120
           GQHILTNPRILGSIVRKSAIRPTDTVLEIGPGTGNLTLKLLEASQKVIAIEIDKRMVEVL
Sbjct: 61  GQHILTNPRILGSIVRKSAIRPTDTVLEIGPGTGNLTLKLLEASQKVIAIEIDKRMVEVL 120

Query: 121 HRRVAENKFEDRICVIREDALKCEFPPFDLVVANIPYGISSPLVAKLVYGAIPFRSATLL 180
           HRRVAE+KFEDRICVIREDALKCEFP FDLVVANIPYGISSPLVAKLVYGAIPFRSATLL
Sbjct: 121 HRRVAEHKFEDRICVIREDALKCEFPRFDLVVANIPYGISSPLVAKLVYGAIPFRSATLL 180

Query: 181 LQKEFARRLLANPGDSEYNRLAVNVKLVADVEHVMDVSKRDFVPCPKVDSSVVIIRPKKE 240
           LQKEFARRLLANPGDSEYNRLAVNVKLVADVEHVMDVSKRDFVPCPKVDSSVVIIRPKKE
Sbjct: 181 LQKEFARRLLANPGDSEYNRLAVNVKLVADVEHVMDVSKRDFVPCPKVDSSVVIIRPKKE 240

Query: 241 VPEVNLEEWRAFTRTCFGKKNKTLGATFKQKRMLMALMNSNSESFNGRGVEEEGCSSSSE 300
           VPEVNLEEWR+FTRTCFGKKNKTLGATFKQKRMLMALMNSNSESFNG+GVEEEGCSSSSE
Sbjct: 241 VPEVNLEEWRSFTRTCFGKKNKTLGATFKQKRMLMALMNSNSESFNGQGVEEEGCSSSSE 300

Query: 301 KGFNLFKEKIIEVLKSGKFEDKRPAKLCNLKLLHLLALFNQAGIYFRDGSELQDAERSAL 360
           KGFN FK KIIE+LKSG FEDKRPAKLCN +LLHLLALFNQAGIYFRDGSELQDAER AL
Sbjct: 301 KGFNSFKAKIIEILKSGSFEDKRPAKLCNEELLHLLALFNQAGIYFRDGSELQDAERRAL 360

Query: 361 ADDAYGSELED 371
           ADDAYGSELED
Sbjct: 361 ADDAYGSELED 371

BLAST of CmaCh07G004580 vs. ExPASy TrEMBL
Match: A0A6J1BU06 (rRNA adenine N(6)-methyltransferase OS=Momordica charantia OX=3673 GN=LOC111005347 PE=3 SV=1)

HSP 1 Score: 593.2 bits (1528), Expect = 7.5e-166
Identity = 312/375 (83.20%), Postives = 335/375 (89.33%), Query Frame = 0

Query: 1   MLRPHKNLCRFINALNHDQFLHCTRSASSYQYNHRNHGEKENV-PKDGNEEPQLYFYKSR 60
           ML PHKNLCRF+  LNH+   H TRSASSYQYN RNHG  ENV  KDGNEE QLYFYKSR
Sbjct: 1   MLSPHKNLCRFVYGLNHNHSFHRTRSASSYQYNQRNHGANENVRTKDGNEEAQLYFYKSR 60

Query: 61  GQHILTNPRILGSIVRKSAIRPTDTVLEIGPGTGNLTLKLLEASQKVIAIEIDKRMVEVL 120
           GQHILTN RIL SIVRKSAIRP+DTVLEIGPGTGNLTLKLLEASQKVIAIEIDKRMVE+L
Sbjct: 61  GQHILTNQRILDSIVRKSAIRPSDTVLEIGPGTGNLTLKLLEASQKVIAIEIDKRMVEIL 120

Query: 121 HRRVAENKFEDRICVIREDALKCEFPPFDLVVANIPYGISSPLVAKLVYGAIPFRSATLL 180
           HRRV E+KFEDRICVI +DALKCEFP FDLVVANIPYGISSPLVAKLVYGAIPFRSATLL
Sbjct: 121 HRRVVEHKFEDRICVICKDALKCEFPRFDLVVANIPYGISSPLVAKLVYGAIPFRSATLL 180

Query: 181 LQKEFARRLLANPGDSEYNRLAVNVKLVADVEHVMDVSKRDFVPCPKVDSSVVIIRPKKE 240
           LQKEFARRLLA+PGDSEYNRLAVNVKLVADVE+VMDVSKR+FVPCPKVDSSVVIIRPKKE
Sbjct: 181 LQKEFARRLLADPGDSEYNRLAVNVKLVADVEYVMDVSKREFVPCPKVDSSVVIIRPKKE 240

Query: 241 VPEVNLEEWRAFTRTCFGKKNKTLGATFKQKRMLMALMN-----SNSESFNGRGVEEEGC 300
           VPEVNLEEWR+FTRTCFGKKN+TLGATFKQKR ++ L++     SNSE+  G   EE+  
Sbjct: 241 VPEVNLEEWRSFTRTCFGKKNRTLGATFKQKRKVIELIHSSKVASNSENSIGGNNEEKSS 300

Query: 301 SSSSEKGFNLFKEKIIEVLKSGKFEDKRPAKLCNLKLLHLLALFNQAGIYFRDGSELQDA 360
           SSSSEKGF+ FKE+IIEVLKSG FEDKRPAKLCN +LLHLLALFN+AGIYF DG+ELQDA
Sbjct: 301 SSSSEKGFDSFKERIIEVLKSGDFEDKRPAKLCNKELLHLLALFNRAGIYFHDGAELQDA 360

Query: 361 ERSALADDAYGSELE 370
           ER +LA DAY SE E
Sbjct: 361 ERRSLA-DAYCSESE 374

BLAST of CmaCh07G004580 vs. ExPASy TrEMBL
Match: A0A5A7SHR6 (rRNA adenine N(6)-methyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold253G002020 PE=3 SV=1)

HSP 1 Score: 578.9 bits (1491), Expect = 1.5e-161
Identity = 304/370 (82.16%), Postives = 321/370 (86.76%), Query Frame = 0

Query: 1   MLRPHKNLCRFINALNHDQFLHCTRSASSYQYNHRNHGEKENVP-KDGNEEPQLYFYKSR 60
           MLRPHKNLCRFI  LNH QFLHC RSAS+YQ+NHRN+G   N+P KD NEE  LYFYKSR
Sbjct: 1   MLRPHKNLCRFIYGLNHHQFLHCIRSASTYQHNHRNNGPHGNLPKKDENEEAHLYFYKSR 60

Query: 61  GQHILTNPRILGSIVRKSAIRPTDTVLEIGPGTGNLTLKLLEASQKVIAIEIDKRMVEVL 120
           GQHILTN RIL SIVRKSAI PTDTVLEIGPGTGNLTLKLLEASQKVIA+EIDKRMVEVL
Sbjct: 61  GQHILTNQRILDSIVRKSAILPTDTVLEIGPGTGNLTLKLLEASQKVIAVEIDKRMVEVL 120

Query: 121 HRRVAENKFEDRICVIREDALKCEFPPFDLVVANIPYGISSPLVAKLVYGAIPFRSATLL 180
           HRRVAE++ EDRICVI +DALKCE P FDLVVANIPYGISSPLVAKLVYG IPFRSATLL
Sbjct: 121 HRRVAEHQLEDRICVICQDALKCELPHFDLVVANIPYGISSPLVAKLVYGTIPFRSATLL 180

Query: 181 LQKEFARRLLANPGDSEYNRLAVNVKLVADVEHVMDVSKRDFVPCPKVDSSVVIIRPKKE 240
           LQKEFARRLLA+PGDSEYNRLAVNVKLVADVEHVMDVSKRDFVPCPKVDSSVV+IRPKKE
Sbjct: 181 LQKEFARRLLADPGDSEYNRLAVNVKLVADVEHVMDVSKRDFVPCPKVDSSVVLIRPKKE 240

Query: 241 VPEVNLEEWRAFTRTCFGKKNKTLGATFKQKRMLMALMNSNSESFNGRGVEEEGCSSSSE 300
           VPEVNLEEWRAFTRTCFGKKNKTLGATFKQKR LM LM+SN                 SE
Sbjct: 241 VPEVNLEEWRAFTRTCFGKKNKTLGATFKQKRKLMDLMHSN-----------------SE 300

Query: 301 KGFNLFKEKIIEVLKSGKFEDKRPAKLCNLKLLHLLALFNQAGIYFRDGSELQDAERSAL 360
           +GFN F+E +I+VLKSG FEDKRP KLCN KLLHLLALFNQ+GIYF + SELQDAERS L
Sbjct: 301 EGFNSFRETVIQVLKSGDFEDKRPVKLCNEKLLHLLALFNQSGIYFHNRSELQDAERSVL 352

Query: 361 ADDAYGSELE 370
           A DAY SE E
Sbjct: 361 A-DAYSSEQE 352

BLAST of CmaCh07G004580 vs. ExPASy TrEMBL
Match: A0A1S4DT73 (rRNA adenine N(6)-methyltransferase OS=Cucumis melo OX=3656 GN=LOC103485093 PE=3 SV=1)

HSP 1 Score: 578.9 bits (1491), Expect = 1.5e-161
Identity = 304/370 (82.16%), Postives = 321/370 (86.76%), Query Frame = 0

Query: 1   MLRPHKNLCRFINALNHDQFLHCTRSASSYQYNHRNHGEKENVP-KDGNEEPQLYFYKSR 60
           MLRPHKNLCRFI  LNH QFLHC RSAS+YQ+NHRN+G   N+P KD NEE  LYFYKSR
Sbjct: 1   MLRPHKNLCRFIYGLNHHQFLHCIRSASTYQHNHRNNGPHGNLPKKDENEEAHLYFYKSR 60

Query: 61  GQHILTNPRILGSIVRKSAIRPTDTVLEIGPGTGNLTLKLLEASQKVIAIEIDKRMVEVL 120
           GQHILTN RIL SIVRKSAI PTDTVLEIGPGTGNLTLKLLEASQKVIA+EIDKRMVEVL
Sbjct: 61  GQHILTNQRILDSIVRKSAILPTDTVLEIGPGTGNLTLKLLEASQKVIAVEIDKRMVEVL 120

Query: 121 HRRVAENKFEDRICVIREDALKCEFPPFDLVVANIPYGISSPLVAKLVYGAIPFRSATLL 180
           HRRVAE++ EDRICVI +DALKCE P FDLVVANIPYGISSPLVAKLVYG IPFRSATLL
Sbjct: 121 HRRVAEHQLEDRICVICQDALKCELPHFDLVVANIPYGISSPLVAKLVYGTIPFRSATLL 180

Query: 181 LQKEFARRLLANPGDSEYNRLAVNVKLVADVEHVMDVSKRDFVPCPKVDSSVVIIRPKKE 240
           LQKEFARRLLA+PGDSEYNRLAVNVKLVADVEHVMDVSKRDFVPCPKVDSSVV+IRPKKE
Sbjct: 181 LQKEFARRLLADPGDSEYNRLAVNVKLVADVEHVMDVSKRDFVPCPKVDSSVVLIRPKKE 240

Query: 241 VPEVNLEEWRAFTRTCFGKKNKTLGATFKQKRMLMALMNSNSESFNGRGVEEEGCSSSSE 300
           VPEVNLEEWRAFTRTCFGKKNKTLGATFKQKR LM LM+SN                 SE
Sbjct: 241 VPEVNLEEWRAFTRTCFGKKNKTLGATFKQKRKLMDLMHSN-----------------SE 300

Query: 301 KGFNLFKEKIIEVLKSGKFEDKRPAKLCNLKLLHLLALFNQAGIYFRDGSELQDAERSAL 360
           +GFN F+E +I+VLKSG FEDKRP KLCN KLLHLLALFNQ+GIYF + SELQDAERS L
Sbjct: 301 EGFNSFRETVIQVLKSGDFEDKRPVKLCNEKLLHLLALFNQSGIYFHNRSELQDAERSVL 352

Query: 361 ADDAYGSELE 370
           A DAY SE E
Sbjct: 361 A-DAYSSEQE 352

BLAST of CmaCh07G004580 vs. NCBI nr
Match: XP_023003077.1 (ribosomal RNA small subunit methyltransferase, mitochondrial [Cucurbita maxima])

HSP 1 Score: 732.3 bits (1889), Expect = 2.1e-207
Identity = 370/370 (100.00%), Postives = 370/370 (100.00%), Query Frame = 0

Query: 1   MLRPHKNLCRFINALNHDQFLHCTRSASSYQYNHRNHGEKENVPKDGNEEPQLYFYKSRG 60
           MLRPHKNLCRFINALNHDQFLHCTRSASSYQYNHRNHGEKENVPKDGNEEPQLYFYKSRG
Sbjct: 1   MLRPHKNLCRFINALNHDQFLHCTRSASSYQYNHRNHGEKENVPKDGNEEPQLYFYKSRG 60

Query: 61  QHILTNPRILGSIVRKSAIRPTDTVLEIGPGTGNLTLKLLEASQKVIAIEIDKRMVEVLH 120
           QHILTNPRILGSIVRKSAIRPTDTVLEIGPGTGNLTLKLLEASQKVIAIEIDKRMVEVLH
Sbjct: 61  QHILTNPRILGSIVRKSAIRPTDTVLEIGPGTGNLTLKLLEASQKVIAIEIDKRMVEVLH 120

Query: 121 RRVAENKFEDRICVIREDALKCEFPPFDLVVANIPYGISSPLVAKLVYGAIPFRSATLLL 180
           RRVAENKFEDRICVIREDALKCEFPPFDLVVANIPYGISSPLVAKLVYGAIPFRSATLLL
Sbjct: 121 RRVAENKFEDRICVIREDALKCEFPPFDLVVANIPYGISSPLVAKLVYGAIPFRSATLLL 180

Query: 181 QKEFARRLLANPGDSEYNRLAVNVKLVADVEHVMDVSKRDFVPCPKVDSSVVIIRPKKEV 240
           QKEFARRLLANPGDSEYNRLAVNVKLVADVEHVMDVSKRDFVPCPKVDSSVVIIRPKKEV
Sbjct: 181 QKEFARRLLANPGDSEYNRLAVNVKLVADVEHVMDVSKRDFVPCPKVDSSVVIIRPKKEV 240

Query: 241 PEVNLEEWRAFTRTCFGKKNKTLGATFKQKRMLMALMNSNSESFNGRGVEEEGCSSSSEK 300
           PEVNLEEWRAFTRTCFGKKNKTLGATFKQKRMLMALMNSNSESFNGRGVEEEGCSSSSEK
Sbjct: 241 PEVNLEEWRAFTRTCFGKKNKTLGATFKQKRMLMALMNSNSESFNGRGVEEEGCSSSSEK 300

Query: 301 GFNLFKEKIIEVLKSGKFEDKRPAKLCNLKLLHLLALFNQAGIYFRDGSELQDAERSALA 360
           GFNLFKEKIIEVLKSGKFEDKRPAKLCNLKLLHLLALFNQAGIYFRDGSELQDAERSALA
Sbjct: 301 GFNLFKEKIIEVLKSGKFEDKRPAKLCNLKLLHLLALFNQAGIYFRDGSELQDAERSALA 360

Query: 361 DDAYGSELED 371
           DDAYGSELED
Sbjct: 361 DDAYGSELED 370

BLAST of CmaCh07G004580 vs. NCBI nr
Match: XP_023517344.1 (ribosomal RNA small subunit methyltransferase, mitochondrial [Cucurbita pepo subsp. pepo])

HSP 1 Score: 703.4 bits (1814), Expect = 1.1e-198
Identity = 356/371 (95.96%), Postives = 362/371 (97.57%), Query Frame = 0

Query: 1   MLRPHKNLCRFINALNHDQFLHCTRSASSYQYNHRNHGEKENVP-KDGNEEPQLYFYKSR 60
           MLRPHKNLCRFIN LNHDQFLHC RSASSYQYNHRNHGEKENVP KDGNEEPQLYFYKSR
Sbjct: 1   MLRPHKNLCRFINGLNHDQFLHCKRSASSYQYNHRNHGEKENVPKKDGNEEPQLYFYKSR 60

Query: 61  GQHILTNPRILGSIVRKSAIRPTDTVLEIGPGTGNLTLKLLEASQKVIAIEIDKRMVEVL 120
           GQHILTNPRILGSIVRKSAI+PTDTVLEIGPGTGNLTLKLLEASQKVIAIEIDKRMVEVL
Sbjct: 61  GQHILTNPRILGSIVRKSAIKPTDTVLEIGPGTGNLTLKLLEASQKVIAIEIDKRMVEVL 120

Query: 121 HRRVAENKFEDRICVIREDALKCEFPPFDLVVANIPYGISSPLVAKLVYGAIPFRSATLL 180
           HRRVAE+KFEDRICVIREDALKCEFP FDLVVANIPYGISSPLVAKLVYGAIPFRSATLL
Sbjct: 121 HRRVAEHKFEDRICVIREDALKCEFPRFDLVVANIPYGISSPLVAKLVYGAIPFRSATLL 180

Query: 181 LQKEFARRLLANPGDSEYNRLAVNVKLVADVEHVMDVSKRDFVPCPKVDSSVVIIRPKKE 240
           LQKEFARRLLANPGDSEYNRLAVNVKLVADVEHVMDVSKRDFVPCPKVDSSVVIIRPKKE
Sbjct: 181 LQKEFARRLLANPGDSEYNRLAVNVKLVADVEHVMDVSKRDFVPCPKVDSSVVIIRPKKE 240

Query: 241 VPEVNLEEWRAFTRTCFGKKNKTLGATFKQKRMLMALMNSNSESFNGRGVEEEGCSSSSE 300
           +PEVNLEEWR+FTRTCFGKKNKTLGATFKQKRMLMALMNSNSESFNGRGVEEEGCSSSSE
Sbjct: 241 IPEVNLEEWRSFTRTCFGKKNKTLGATFKQKRMLMALMNSNSESFNGRGVEEEGCSSSSE 300

Query: 301 KGFNLFKEKIIEVLKSGKFEDKRPAKLCNLKLLHLLALFNQAGIYFRDGSELQDAERSAL 360
           KGFN FKEKIIE+LKSG FEDKRPAKLCN + LHLLALFNQAGIYFRDGSELQDAER AL
Sbjct: 301 KGFNSFKEKIIEILKSGSFEDKRPAKLCNEEFLHLLALFNQAGIYFRDGSELQDAERRAL 360

Query: 361 ADDAYGSELED 371
           ADDAYGSELED
Sbjct: 361 ADDAYGSELED 371

BLAST of CmaCh07G004580 vs. NCBI nr
Match: KAG7026768.1 (Ribosomal RNA small subunit methyltransferase, mitochondrial, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 700.7 bits (1807), Expect = 6.9e-198
Identity = 355/371 (95.69%), Postives = 361/371 (97.30%), Query Frame = 0

Query: 1   MLRPHKNLCRFINALNHDQFLHCTRSASSYQYNHRNHGEKENVP-KDGNEEPQLYFYKSR 60
           MLRPHKNLCRFIN LNHDQFLHC RSASSYQYNHRNHG KENVP KDGNEEPQLYFYKSR
Sbjct: 1   MLRPHKNLCRFINGLNHDQFLHCKRSASSYQYNHRNHGGKENVPKKDGNEEPQLYFYKSR 60

Query: 61  GQHILTNPRILGSIVRKSAIRPTDTVLEIGPGTGNLTLKLLEASQKVIAIEIDKRMVEVL 120
           GQHILTNPRILG IVRKSA+RPTDTVLEIGPGTGNLTLKLLEASQKVIAIEIDKRMVEVL
Sbjct: 61  GQHILTNPRILGFIVRKSAVRPTDTVLEIGPGTGNLTLKLLEASQKVIAIEIDKRMVEVL 120

Query: 121 HRRVAENKFEDRICVIREDALKCEFPPFDLVVANIPYGISSPLVAKLVYGAIPFRSATLL 180
           H+RVAE+KFEDRICVIREDALKCEFPPFDLVVANIPYGISSPLVAKLVYGAIPFRSATLL
Sbjct: 121 HKRVAEHKFEDRICVIREDALKCEFPPFDLVVANIPYGISSPLVAKLVYGAIPFRSATLL 180

Query: 181 LQKEFARRLLANPGDSEYNRLAVNVKLVADVEHVMDVSKRDFVPCPKVDSSVVIIRPKKE 240
           LQKEFARRLLANPGDSEYNRLAVNVKLVADVEHVMDVSKRDFVPCPKVDSSVVIIRPKKE
Sbjct: 181 LQKEFARRLLANPGDSEYNRLAVNVKLVADVEHVMDVSKRDFVPCPKVDSSVVIIRPKKE 240

Query: 241 VPEVNLEEWRAFTRTCFGKKNKTLGATFKQKRMLMALMNSNSESFNGRGVEEEGCSSSSE 300
           VPEVNLEEWR+FTRTCFGKKNKTLGATFKQKRMLMALMNSNSESFNG GVEEEGCSSSSE
Sbjct: 241 VPEVNLEEWRSFTRTCFGKKNKTLGATFKQKRMLMALMNSNSESFNGGGVEEEGCSSSSE 300

Query: 301 KGFNLFKEKIIEVLKSGKFEDKRPAKLCNLKLLHLLALFNQAGIYFRDGSELQDAERSAL 360
           KGFN FKEKIIE+LKSG FEDKRPAKLCN +LLHLLALFNQAGIYFRDGSELQDAER AL
Sbjct: 301 KGFNSFKEKIIEILKSGSFEDKRPAKLCNEELLHLLALFNQAGIYFRDGSELQDAERRAL 360

Query: 361 ADDAYGSELED 371
           ADDAYGSELED
Sbjct: 361 ADDAYGSELED 371

BLAST of CmaCh07G004580 vs. NCBI nr
Match: XP_022963244.1 (ribosomal RNA small subunit methyltransferase, mitochondrial [Cucurbita moschata])

HSP 1 Score: 699.9 bits (1805), Expect = 1.2e-197
Identity = 356/371 (95.96%), Postives = 361/371 (97.30%), Query Frame = 0

Query: 1   MLRPHKNLCRFINALNHDQFLHCTRSASSYQYNHRNHGEKENVP-KDGNEEPQLYFYKSR 60
           MLRPHKNLCRFIN LNHDQFLHC RSASSYQYNHRNHG KENVP KDGNEEPQLYFYKSR
Sbjct: 1   MLRPHKNLCRFINGLNHDQFLHCKRSASSYQYNHRNHGGKENVPKKDGNEEPQLYFYKSR 60

Query: 61  GQHILTNPRILGSIVRKSAIRPTDTVLEIGPGTGNLTLKLLEASQKVIAIEIDKRMVEVL 120
           GQHILTNPRILGSIVRKSAIRPTDTVLEIGPGTGNLTLKLLEASQKVIAIEIDKRMVEVL
Sbjct: 61  GQHILTNPRILGSIVRKSAIRPTDTVLEIGPGTGNLTLKLLEASQKVIAIEIDKRMVEVL 120

Query: 121 HRRVAENKFEDRICVIREDALKCEFPPFDLVVANIPYGISSPLVAKLVYGAIPFRSATLL 180
           HRRVAE+KFEDRICVIREDALKCEFP FDLVVANIPYGISSPLVAKLVYGAIPFRSATLL
Sbjct: 121 HRRVAEHKFEDRICVIREDALKCEFPRFDLVVANIPYGISSPLVAKLVYGAIPFRSATLL 180

Query: 181 LQKEFARRLLANPGDSEYNRLAVNVKLVADVEHVMDVSKRDFVPCPKVDSSVVIIRPKKE 240
           LQKEFARRLLANPGDSEYNRLAVNVKLVADVEHVMDVSKRDFVPCPKVDSSVVIIRPKKE
Sbjct: 181 LQKEFARRLLANPGDSEYNRLAVNVKLVADVEHVMDVSKRDFVPCPKVDSSVVIIRPKKE 240

Query: 241 VPEVNLEEWRAFTRTCFGKKNKTLGATFKQKRMLMALMNSNSESFNGRGVEEEGCSSSSE 300
           VPEVNLEEWR+FTRTCFGKKNKTLGATFKQKRMLMALMNSNSESFNG+GVEEEGCSSSSE
Sbjct: 241 VPEVNLEEWRSFTRTCFGKKNKTLGATFKQKRMLMALMNSNSESFNGQGVEEEGCSSSSE 300

Query: 301 KGFNLFKEKIIEVLKSGKFEDKRPAKLCNLKLLHLLALFNQAGIYFRDGSELQDAERSAL 360
           KGFN FK KIIE+LKSG FEDKRPAKLCN +LLHLLALFNQAGIYFRDGSELQDAER AL
Sbjct: 301 KGFNSFKAKIIEILKSGSFEDKRPAKLCNEELLHLLALFNQAGIYFRDGSELQDAERRAL 360

Query: 361 ADDAYGSELED 371
           ADDAYGSELED
Sbjct: 361 ADDAYGSELED 371

BLAST of CmaCh07G004580 vs. NCBI nr
Match: KAG6594800.1 (Ribosomal RNA small subunit methyltransferase, mitochondrial, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 698.7 bits (1802), Expect = 2.6e-197
Identity = 356/371 (95.96%), Postives = 360/371 (97.04%), Query Frame = 0

Query: 1   MLRPHKNLCRFINALNHDQFLHCTRSASSYQYNHRNHGEKENVP-KDGNEEPQLYFYKSR 60
           MLRPHKNLCRFIN LNHDQFLHC RSASSYQYNHRNHG KENVP KDGNEEPQLYFYKSR
Sbjct: 1   MLRPHKNLCRFINGLNHDQFLHCKRSASSYQYNHRNHGGKENVPKKDGNEEPQLYFYKSR 60

Query: 61  GQHILTNPRILGSIVRKSAIRPTDTVLEIGPGTGNLTLKLLEASQKVIAIEIDKRMVEVL 120
           GQHILTNPRILGSIVRKSAIRPTDTVLEIGPGTGNLTLKLLEASQKVIAIEIDKRMVEVL
Sbjct: 61  GQHILTNPRILGSIVRKSAIRPTDTVLEIGPGTGNLTLKLLEASQKVIAIEIDKRMVEVL 120

Query: 121 HRRVAENKFEDRICVIREDALKCEFPPFDLVVANIPYGISSPLVAKLVYGAIPFRSATLL 180
           HRRVAE+KFEDRICVIREDALKCEFP FDLVVANIPYGISSPLVAKLVYGAIPFRSATLL
Sbjct: 121 HRRVAEHKFEDRICVIREDALKCEFPRFDLVVANIPYGISSPLVAKLVYGAIPFRSATLL 180

Query: 181 LQKEFARRLLANPGDSEYNRLAVNVKLVADVEHVMDVSKRDFVPCPKVDSSVVIIRPKKE 240
           LQKEFARRLLANPGDSEYNRLAVNVKLVADV HVMDVSKRDFVPCPKVDSSVVIIRPKKE
Sbjct: 181 LQKEFARRLLANPGDSEYNRLAVNVKLVADVAHVMDVSKRDFVPCPKVDSSVVIIRPKKE 240

Query: 241 VPEVNLEEWRAFTRTCFGKKNKTLGATFKQKRMLMALMNSNSESFNGRGVEEEGCSSSSE 300
           VPEVNLEEWR+FTRTCFGKKNKTLGATFKQKRMLMALMNSNSESFNG GVEEEGCSSSSE
Sbjct: 241 VPEVNLEEWRSFTRTCFGKKNKTLGATFKQKRMLMALMNSNSESFNGGGVEEEGCSSSSE 300

Query: 301 KGFNLFKEKIIEVLKSGKFEDKRPAKLCNLKLLHLLALFNQAGIYFRDGSELQDAERSAL 360
           KGFN FKEKIIE+LKSG FEDKRPAKLCN +LLHLLALFNQAGIYFRDGSELQDAER AL
Sbjct: 301 KGFNSFKEKIIEILKSGSFEDKRPAKLCNEELLHLLALFNQAGIYFRDGSELQDAERRAL 360

Query: 361 ADDAYGSELED 371
           ADDAYGSELED
Sbjct: 361 ADDAYGSELED 371

BLAST of CmaCh07G004580 vs. TAIR 10
Match: AT5G66360.2 (Ribosomal RNA adenine dimethylase family protein )

HSP 1 Score: 386.7 bits (992), Expect = 2.1e-107
Identity = 205/337 (60.83%), Postives = 263/337 (78.04%), Query Frame = 0

Query: 32  YNHRNHGEKENVP-KDGNEEPQLYFYKSRGQHILTNPRILGSIVRKSAIRPTDTVLEIGP 91
           ++ R  G + +V  ++  E   L+  KS+GQH+LTN RIL SIVR S IRPTDTVLEIGP
Sbjct: 39  HDRRRRGYERDVRIEEKKEHDGLFLCKSKGQHLLTNTRILDSIVRSSDIRPTDTVLEIGP 98

Query: 92  GTGNLTLKLLEASQKVIAIEIDKRMVEVLHRRVAENKFEDRICVIREDALKCEFPPFDLV 151
           GTGNLT+KLLEA+Q V+A+E+DKRMVE+L +RV+++ F D++ +I++D LK +FP FDLV
Sbjct: 99  GTGNLTMKLLEAAQNVVAVELDKRMVEILRKRVSDHGFADKLTIIQKDVLKTDFPHFDLV 158

Query: 152 VANIPYGISSPLVAKLVYGAIPFRSATLLLQKEFARRLLANPGDSEYNRLAVNVKLVADV 211
           VANIPY ISSPLVAKLVYG+  FRSATLLLQKEF+RRLLANPGDS++NRLAVNVKLVADV
Sbjct: 159 VANIPYNISSPLVAKLVYGSNTFRSATLLLQKEFSRRLLANPGDSDFNRLAVNVKLVADV 218

Query: 212 EHVMDVSKRDFVPCPKVDSSVVIIRPKKEVPEVNLEEWRAFTRTCFGKKNKTLGATFKQK 271
           + VMDVSKR+FVP PKVDSSV+ I PK+ +P+VN++EW AFTRTCFGKKNKTLG+ F+QK
Sbjct: 219 KFVMDVSKREFVPPPKVDSSVIRITPKEIIPDVNVQEWLAFTRTCFGKKNKTLGSMFRQK 278

Query: 272 RMLMALMN-------SNSESFNGRG------VEEEGCSS--SSEKGFNLFKEKIIEVLKS 331
           + +M L +       SN E  N  G      VEE+G       +   ++FKE++IE+L++
Sbjct: 279 KKVMELQSLSAGRHGSNVEVMNQTGGDSDSDVEEDGKDDLLCLDTDASMFKERVIEILRT 338

Query: 332 GKFEDKRPAKLCNLKLLHLLALFNQAGIYFRDGSELQ 353
             FE+KRP+KL + +LLHLL+LFNQAGI+F D + LQ
Sbjct: 339 NGFEEKRPSKLSHRELLHLLSLFNQAGIFFHDITSLQ 375

BLAST of CmaCh07G004580 vs. TAIR 10
Match: AT5G66360.1 (Ribosomal RNA adenine dimethylase family protein )

HSP 1 Score: 332.0 bits (850), Expect = 6.0e-91
Identity = 183/337 (54.30%), Postives = 238/337 (70.62%), Query Frame = 0

Query: 32  YNHRNHGEKENVP-KDGNEEPQLYFYKSRGQHILTNPRILGSIVRKSAIRPTDTVLEIGP 91
           ++ R  G + +V  ++  E   L+  KS+GQH+LTN RIL SIVR S IRPTDTVLEIGP
Sbjct: 39  HDRRRRGYERDVRIEEKKEHDGLFLCKSKGQHLLTNTRILDSIVRSSDIRPTDTVLEIGP 98

Query: 92  GTGNLTLKLLEASQKVIAIEIDKRMVEVLHRRVAENKFEDRICVIREDALKCEFPPFDLV 151
           GTGNLT+KLLEA+Q V+A+E+DKRMVE+L +RV+++ F D++ +I++D LK +FP FDLV
Sbjct: 99  GTGNLTMKLLEAAQNVVAVELDKRMVEILRKRVSDHGFADKLTIIQKDVLKTDFPHFDLV 158

Query: 152 VANIPYGISSPLVAKLVYGAIPFRSATLLLQKEFARRLLANPGDSEYNRLAVNVKLVADV 211
           VANIPY ISSPLVAKLVYG+  FRSATLLLQKEF+RRLLANPGDS++NRLAVNVK     
Sbjct: 159 VANIPYNISSPLVAKLVYGSNTFRSATLLLQKEFSRRLLANPGDSDFNRLAVNVK----- 218

Query: 212 EHVMDVSKRDFVPCPKVDSSVVIIRPKKEVPEVNLEEWRAFTRTCFGKKNKTLGATFKQK 271
                                  I PK+ +P+VN++EW AFTRTCFGKKNKTLG+ F+QK
Sbjct: 219 -----------------------ITPKEIIPDVNVQEWLAFTRTCFGKKNKTLGSMFRQK 278

Query: 272 RMLMALMN-------SNSESFNGRG------VEEEGCSS--SSEKGFNLFKEKIIEVLKS 331
           + +M L +       SN E  N  G      VEE+G       +   ++FKE++IE+L++
Sbjct: 279 KKVMELQSLSAGRHGSNVEVMNQTGGDSDSDVEEDGKDDLLCLDTDASMFKERVIEILRT 338

Query: 332 GKFEDKRPAKLCNLKLLHLLALFNQAGIYFRDGSELQ 353
             FE+KRP+KL + +LLHLL+LFNQAGI+F D + LQ
Sbjct: 339 NGFEEKRPSKLSHRELLHLLSLFNQAGIFFHDITSLQ 347

BLAST of CmaCh07G004580 vs. TAIR 10
Match: AT2G47420.1 (Ribosomal RNA adenine dimethylase family protein )

HSP 1 Score: 273.9 bits (699), Expect = 1.9e-73
Identity = 159/348 (45.69%), Postives = 213/348 (61.21%), Query Frame = 0

Query: 40  KENVPKDGNEEPQ------LYFYKSRGQHILTNPRILGSIVRKSAIRPTDTVLEIGPGTG 99
           ++  PK  N  P       + F+KS+GQHIL NP ++ SIV+K+ I+ TD +LEIGPGTG
Sbjct: 7   RKEKPKASNRAPSNHYQGGISFHKSKGQHILKNPLLVDSIVQKAGIKSTDVILEIGPGTG 66

Query: 100 NLTLKLLEASQKVIAIEIDKRMVEVLHRRVAENKFEDRICVIREDALKCEFPPFDLVVAN 159
           NLT KLLEA ++VIA+E+D RMV  L RR     F +R+ VI+ D LK E P FD+ VAN
Sbjct: 67  NLTKKLLEAGKEVIAVELDSRMVLELQRRFQGTPFSNRLKVIQGDVLKTELPRFDICVAN 126

Query: 160 IPYGISSPLVAKLVYGAIPFRSATLLLQKEFARRLLANPGDSEYNRLAVNVKLVADVEHV 219
           IPY ISSPL  KL++    FR A ++ Q+EFA RL+A PGD+ Y RL+VN +L A V H+
Sbjct: 127 IPYQISSPLTFKLLFHPTSFRCAVIMYQREFAMRLVAQPGDNLYCRLSVNTQLYARVSHL 186

Query: 220 MDVSKRDFVPCPKVDSSVVIIRPKKEVPEVNLEEWRAFTRTCFGKKNKTLGATFKQK--- 279
           + V K +F P PKVDSSVV I P++  P+VN +EW  F R CF +KNKTLG+ FKQK   
Sbjct: 187 LKVGKNNFRPPPKVDSSVVRIEPRRPGPQVNKKEWDGFLRVCFIRKNKTLGSIFKQKSVL 246

Query: 280 -----------RMLMALMNSNSESFN---------GRGVE-------------EEGCSSS 339
                       +L +L N+   + N           G+E             +EG    
Sbjct: 247 SMLEKNFKTLQAVLASLQNNGEPALNTTSMDLGDQSMGMEDDDNEMDDDDMEMDEGEGDG 306

Query: 340 SEKGFNLFKEKIIEVLKSGKFEDKRPAKLCNLKLLHLLALFNQAGIYF 346
            E   + FKEK++ VLK G FE+KR +KL   + L+LL+LFN++GI+F
Sbjct: 307 GET--SEFKEKVMNVLKEGGFEEKRSSKLSQQEFLYLLSLFNKSGIHF 352

BLAST of CmaCh07G004580 vs. TAIR 10
Match: AT1G01860.1 (Ribosomal RNA adenine dimethylase family protein )

HSP 1 Score: 98.6 bits (244), Expect = 1.1e-20
Identity = 73/228 (32.02%), Postives = 115/228 (50.44%), Query Frame = 0

Query: 57  KSRGQHILTNPRILGSIVRKSAIRPTDTVLEIGPGTGNLTLKLLEASQKVIAIEIDKRMV 116
           KS GQH + N  I   +   + ++  D VLEIGPGTG+LT  L+     V+AIE D  MV
Sbjct: 73  KSLGQHYMLNSDINDQLASAADVKEGDFVLEIGPGTGSLTNVLINLGATVLAIEKDPHMV 132

Query: 117 EVLHRRVAENKFEDRICVIREDALKCEFPPFDL-----------------VVANIPYGIS 176
           +++  R A +   D+  V++ED +KC      L                 VV+N+P+ IS
Sbjct: 133 DLVSERFAGS---DKFKVLQEDFVKCHIRSHMLSILETRRLSHPDSALAKVVSNLPFNIS 192

Query: 177 SPLVAKLVYGAIPFRSATLLLQKEFARRLLANP-GDSEYNRLAVNVKLVADVEHVMDVSK 236
           + +V  L+     F    LLLQ E A RL+      SEY  + + +   ++ E+   V +
Sbjct: 193 TDVVKLLLPMGDIFSKVVLLLQDEAALRLVEPALRTSEYRPINILINFYSEPEYNFRVPR 252

Query: 237 RDFVPCPKVDSSVVIIRPK--KEVPEV-NLEEWRAFTRTCFGKKNKTL 264
            +F P PKVD++VV  + K  ++ P+V + + + +   + F  K K L
Sbjct: 253 ENFFPQPKVDAAVVTFKLKHPRDYPDVSSTKNFFSLVNSAFNGKRKML 297

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9FK022.9e-10660.83Ribosomal RNA small subunit methyltransferase, mitochondrial OS=Arabidopsis thal... [more]
O222682.7e-7245.69Ribosomal RNA small subunit methyltransferase OS=Arabidopsis thaliana OX=3702 GN... [more]
Q54QK73.1e-6046.55Probable dimethyladenosine transferase OS=Dictyostelium discoideum OX=44689 GN=d... [more]
Q2KHT81.3e-5845.27Probable dimethyladenosine transferase OS=Bos taurus OX=9913 GN=DIMT1 PE=2 SV=1[more]
Q9UNQ22.2e-5844.93Probable dimethyladenosine transferase OS=Homo sapiens OX=9606 GN=DIMT1 PE=1 SV=... [more]
Match NameE-valueIdentityDescription
A0A6J1KLE61.0e-207100.00rRNA adenine N(6)-methyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111496791 ... [more]
A0A6J1HJI55.7e-19895.96rRNA adenine N(6)-methyltransferase OS=Cucurbita moschata OX=3662 GN=LOC11146351... [more]
A0A6J1BU067.5e-16683.20rRNA adenine N(6)-methyltransferase OS=Momordica charantia OX=3673 GN=LOC1110053... [more]
A0A5A7SHR61.5e-16182.16rRNA adenine N(6)-methyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E6... [more]
A0A1S4DT731.5e-16182.16rRNA adenine N(6)-methyltransferase OS=Cucumis melo OX=3656 GN=LOC103485093 PE=3... [more]
Match NameE-valueIdentityDescription
XP_023003077.12.1e-207100.00ribosomal RNA small subunit methyltransferase, mitochondrial [Cucurbita maxima][more]
XP_023517344.11.1e-19895.96ribosomal RNA small subunit methyltransferase, mitochondrial [Cucurbita pepo sub... [more]
KAG7026768.16.9e-19895.69Ribosomal RNA small subunit methyltransferase, mitochondrial, partial [Cucurbita... [more]
XP_022963244.11.2e-19795.96ribosomal RNA small subunit methyltransferase, mitochondrial [Cucurbita moschata... [more]
KAG6594800.12.6e-19795.96Ribosomal RNA small subunit methyltransferase, mitochondrial, partial [Cucurbita... [more]
Match NameE-valueIdentityDescription
AT5G66360.22.1e-10760.83Ribosomal RNA adenine dimethylase family protein [more]
AT5G66360.16.0e-9154.30Ribosomal RNA adenine dimethylase family protein [more]
AT2G47420.11.9e-7345.69Ribosomal RNA adenine dimethylase family protein [more]
AT1G01860.11.1e-2032.02Ribosomal RNA adenine dimethylase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR020598Ribosomal RNA adenine methylase transferase, N-terminalSMARTSM00650rADcneu6coord: 69..238
e-value: 5.4E-78
score: 275.1
NoneNo IPR availableGENE3D1.10.8.480coord: 241..346
e-value: 2.2E-27
score: 97.6
NoneNo IPR availableGENE3D3.40.50.150Vaccinia Virus protein VP39coord: 55..240
e-value: 9.4E-63
score: 213.2
NoneNo IPR availablePANTHERPTHR11727:SF12RIBOSOMAL RNA SMALL SUBUNIT METHYLTRANSFERASE, MITOCHONDRIALcoord: 11..345
NoneNo IPR availableCDDcd02440AdoMet_MTasescoord: 84..167
e-value: 7.80233E-12
score: 59.3658
IPR011530Ribosomal RNA adenine dimethylaseTIGRFAMTIGR00755TIGR00755coord: 56..335
e-value: 5.1E-70
score: 233.7
IPR001737Ribosomal RNA adenine methyltransferase KsgA/ErmPFAMPF00398RrnaADcoord: 57..270
e-value: 2.1E-58
score: 197.5
IPR001737Ribosomal RNA adenine methyltransferase KsgA/ErmPANTHERPTHR11727DIMETHYLADENOSINE TRANSFERASEcoord: 11..345
IPR001737Ribosomal RNA adenine methyltransferase KsgA/ErmPROSITEPS51689SAM_RNA_A_N6_MTcoord: 55..342
score: 63.057426
IPR020596Ribosomal RNA adenine methylase transferase, conserved sitePROSITEPS01131RRNA_A_DIMETHcoord: 85..112
IPR029063S-adenosyl-L-methionine-dependent methyltransferaseSUPERFAMILY53335S-adenosyl-L-methionine-dependent methyltransferasescoord: 54..269

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh07G004580.1CmaCh07G004580.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0031167 rRNA methylation
biological_process GO:0000154 rRNA modification
biological_process GO:0006364 rRNA processing
cellular_component GO:0005759 mitochondrial matrix
molecular_function GO:0003723 RNA binding
molecular_function GO:0000179 rRNA (adenine-N6,N6-)-dimethyltransferase activity
molecular_function GO:0008649 rRNA methyltransferase activity