CmaCh07G003910 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh07G003910
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Descriptionendochitinase-like
LocationCma_Chr07: 1682075 .. 1683961 (-)
RNA-Seq ExpressionCmaCh07G003910
SyntenyCmaCh07G003910
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AATTGGGGCACTTCTCTTCTCATCTTCTTCATCAACAACCCATTTCCTTATTTTTCTTCTATAATCTCTGGTTTTCAGAGATGAAAAGAATGAAGCTTCATGCATTCATCTTCTTATCCCTTGCTTTGCTGTTGAGCGCCTCTGCCCAGCAATGTGGGCGGCAAGCCAACAGCGCTGTTTGTCAAAATGGGTTGTGTTGCAGCGAGCACGGCTGGTGCGGCACCACCGACGCCTACTGTGGGGCTGGTTGTCAAAGCCAGTGTCGCCGTCCTGGCCCAACTCCAACCCTCCCCCCTAATGCTGGATCTGGTGTCGGCCGGATCATCACAGCCGCTGTCTTCGATCAATTGCTCAAGTACCGTAACGACCCACGTTGTCGTAGTAATGGGTTCTATACTTACAATGCTTTCCTTACCGCTGCTCGATCCTTCCCTGCCTTCGGTACGACCGGAGATGATGCAACCCGTAGAAGGGAGCTAGCAGCTTTCCTAGCCCAAACTTCTCACGAGACCACCGGTTAGTTTATTAAGTTTTATCCATAAGAAATCACCCATATATTATCCACTTTAAGTATAAACTGAGTAGCTTTGTTTTGGGCTTCCCAAAAATGTCTCATGTCAATAGAAACGTATTTCTTATTTATAAACACATGATCATTCCCTAAGTTAGCCATCGAACAAACTACATCATTTGTTCGACATTTGAGAGCCCTCAAACAGCCTCCATTTAATCGAGGTTCGACTTCTCCTCTAGAATTCTCAAATAAAGTACACCATTTGTTCGAACTACACCTTCAAGGCTCACAAATTCTTTATTCAATATTTAAAGATTCTATTAACATGACTAAGTTAAAGAACATGACATGATACCATAGGAATCACGGATCTCCACAATAGTATGATATTGTCCATTTTGAGCATAAACTCTTATGACTTTGGTTTGGACTTTTCCGTAAAGCCTTATGCTAATGAAGATGTATTCCTTAATCGTGAGACTCCTTTCTACCAATCCTCAACATTATCAAATTATAAGAACAAAATTCTAATAAATAACGAGAGATTGTGATACAGGAGGATGGCCAACTGCACCCGACGGTCCATACGCGTGGGGATATTGTTTTGTTAGAGAGCTCCGACAAGATGCCTACTGTAGATCACCTGATCCGGAATGGCCATGCGCTCCCGGTCAGAAATACTTTGGTCGTGGACCCATTCAACTCACTCAGTAAGGAATTACCCTCTCTATCCTTCTCCCTTATGGCTTGTTTTATTATACTATTGTGGAGAGTCGTAATTCTTAACCTACTTAACGTTGATATCTTATATGATGCTAGCAACTACAACTACGGGCAGGCTGGGAGAGCGTTGGATTTGAATTTACTGAACAACCCGGACTTGGTAGCCACAGACCCAATTGTGTCGTTCAGAACAGCCATTTGGTTTTGGATGACACCACAAGGAAACAAGCCATCGAGCCATGATGTGATCATCGGAAGGTGGCAGCCATCGAATGTTGATAGAGCTGCTGGAAGAGCTGCTGGCTATGGTGTTATTACCAACATCATCAACGGTGAATTTGAATGTGGGAGCGGTCCTAACGATAGAGCCACCAGTAGAATTGGATTTTATACGAGTTACTGCAACTTGTTGGGTGTCACCATGGGACCAAACTTAGACTGCAACAATCAAAGGCCTTTCGGGTCCCGATAAAACCATCTCCACGTACCCCTCATAAAAACATCTATCTTTCTTGATATCCAATTAGTTTGATCTTTGTATGATTTAAATAATATCATAGCATTCTCTACTTTAATCTGTTACGTATCATTGTCAGTTTATTACTGACAAATATTATCTATTTTAACTCATTGTGTATCATCGTCAGTCT

mRNA sequence

AATTGGGGCACTTCTCTTCTCATCTTCTTCATCAACAACCCATTTCCTTATTTTTCTTCTATAATCTCTGGTTTTCAGAGATGAAAAGAATGAAGCTTCATGCATTCATCTTCTTATCCCTTGCTTTGCTGTTGAGCGCCTCTGCCCAGCAATGTGGGCGGCAAGCCAACAGCGCTGTTTGTCAAAATGGGTTGTGTTGCAGCGAGCACGGCTGGTGCGGCACCACCGACGCCTACTGTGGGGCTGGTTGTCAAAGCCAGTGTCGCCGTCCTGGCCCAACTCCAACCCTCCCCCCTAATGCTGGATCTGGTGTCGGCCGGATCATCACAGCCGCTGTCTTCGATCAATTGCTCAAGTACCGTAACGACCCACGTTGTCGTAGTAATGGGTTCTATACTTACAATGCTTTCCTTACCGCTGCTCGATCCTTCCCTGCCTTCGGTACGACCGGAGATGATGCAACCCGTAGAAGGGAGCTAGCAGCTTTCCTAGCCCAAACTTCTCACGAGACCACCGGAGGATGGCCAACTGCACCCGACGGTCCATACGCGTGGGGATATTGTTTTGTTAGAGAGCTCCGACAAGATGCCTACTGTAGATCACCTGATCCGGAATGGCCATGCGCTCCCGGTCAGAAATACTTTGGTCGTGGACCCATTCAACTCACTCACAACTACAACTACGGGCAGGCTGGGAGAGCGTTGGATTTGAATTTACTGAACAACCCGGACTTGGTAGCCACAGACCCAATTGTGTCGTTCAGAACAGCCATTTGGTTTTGGATGACACCACAAGGAAACAAGCCATCGAGCCATGATGTGATCATCGGAAGGTGGCAGCCATCGAATGTTGATAGAGCTGCTGGAAGAGCTGCTGGCTATGGTGTTATTACCAACATCATCAACGGTGAATTTGAATGTGGGAGCGGTCCTAACGATAGAGCCACCAGTAGAATTGGATTTTATACGAGTTACTGCAACTTGTTGGGTGTCACCATGGGACCAAACTTAGACTGCAACAATCAAAGGCCTTTCGGGTCCCGATAAAACCATCTCCACGTACCCCTCATAAAAACATCTATCTTTCTTGATATCCAATTAGTTTGATCTTTGTATGATTTAAATAATATCATAGCATTCTCTACTTTAATCTGTTACGTATCATTGTCAGTTTATTACTGACAAATATTATCTATTTTAACTCATTGTGTATCATCGTCAGTCT

Coding sequence (CDS)

ATGAAAAGAATGAAGCTTCATGCATTCATCTTCTTATCCCTTGCTTTGCTGTTGAGCGCCTCTGCCCAGCAATGTGGGCGGCAAGCCAACAGCGCTGTTTGTCAAAATGGGTTGTGTTGCAGCGAGCACGGCTGGTGCGGCACCACCGACGCCTACTGTGGGGCTGGTTGTCAAAGCCAGTGTCGCCGTCCTGGCCCAACTCCAACCCTCCCCCCTAATGCTGGATCTGGTGTCGGCCGGATCATCACAGCCGCTGTCTTCGATCAATTGCTCAAGTACCGTAACGACCCACGTTGTCGTAGTAATGGGTTCTATACTTACAATGCTTTCCTTACCGCTGCTCGATCCTTCCCTGCCTTCGGTACGACCGGAGATGATGCAACCCGTAGAAGGGAGCTAGCAGCTTTCCTAGCCCAAACTTCTCACGAGACCACCGGAGGATGGCCAACTGCACCCGACGGTCCATACGCGTGGGGATATTGTTTTGTTAGAGAGCTCCGACAAGATGCCTACTGTAGATCACCTGATCCGGAATGGCCATGCGCTCCCGGTCAGAAATACTTTGGTCGTGGACCCATTCAACTCACTCACAACTACAACTACGGGCAGGCTGGGAGAGCGTTGGATTTGAATTTACTGAACAACCCGGACTTGGTAGCCACAGACCCAATTGTGTCGTTCAGAACAGCCATTTGGTTTTGGATGACACCACAAGGAAACAAGCCATCGAGCCATGATGTGATCATCGGAAGGTGGCAGCCATCGAATGTTGATAGAGCTGCTGGAAGAGCTGCTGGCTATGGTGTTATTACCAACATCATCAACGGTGAATTTGAATGTGGGAGCGGTCCTAACGATAGAGCCACCAGTAGAATTGGATTTTATACGAGTTACTGCAACTTGTTGGGTGTCACCATGGGACCAAACTTAGACTGCAACAATCAAAGGCCTTTCGGGTCCCGATAA

Protein sequence

MKRMKLHAFIFLSLALLLSASAQQCGRQANSAVCQNGLCCSEHGWCGTTDAYCGAGCQSQCRRPGPTPTLPPNAGSGVGRIITAAVFDQLLKYRNDPRCRSNGFYTYNAFLTAARSFPAFGTTGDDATRRRELAAFLAQTSHETTGGWPTAPDGPYAWGYCFVRELRQDAYCRSPDPEWPCAPGQKYFGRGPIQLTHNYNYGQAGRALDLNLLNNPDLVATDPIVSFRTAIWFWMTPQGNKPSSHDVIIGRWQPSNVDRAAGRAAGYGVITNIINGEFECGSGPNDRATSRIGFYTSYCNLLGVTMGPNLDCNNQRPFGSR
Homology
BLAST of CmaCh07G003910 vs. ExPASy Swiss-Prot
Match: P36907 (Endochitinase OS=Pisum sativum OX=3888 PE=2 SV=1)

HSP 1 Score: 462.6 bits (1189), Expect = 3.6e-129
Identity = 215/322 (66.77%), Postives = 253/322 (78.57%), Query Frame = 0

Query: 1   MKR-MKLHAFIFLSLALLLSASAQQCGRQANSAVCQNGLCCSEHGWCGTTDAYCGAGCQS 60
           MKR +K+  FI   L L L + A+QCG QA  AVC NGLCCS+ G+CG+TD YCG GCQS
Sbjct: 1   MKRTLKVSFFILCLLPLFLGSKAEQCGSQAGGAVCPNGLCCSKFGFCGSTDPYCGDGCQS 60

Query: 61  QCRRPGPTPTLP-PNAGSG-VGRIITAAVFDQLLKYRNDPRCRSNGFYTYNAFLTAARSF 120
           QC +  PTPT+P P+ G G VGR++ +++FDQ+LKYRND RC  +GFYTY+AF+ AARSF
Sbjct: 61  QC-KSSPTPTIPTPSTGGGDVGRLVPSSLFDQMLKYRNDGRCAGHGFYTYDAFIAAARSF 120

Query: 121 PAFGTTGDDATRRRELAAFLAQTSHETTGGWPTAPDGPYAWGYCFVRELR-QDAYCRSPD 180
             FGTTGDD T+++ELAAFLAQTSHETTGGWPTAPDGPYAWGYCFV E   Q+ YC   D
Sbjct: 121 NGFGTTGDDNTKKKELAAFLAQTSHETTGGWPTAPDGPYAWGYCFVSEQNTQEVYCSPKD 180

Query: 181 PEWPCAPGQKYFGRGPIQLTHNYNYGQAGRALDLNLLNNPDLVATDPIVSFRTAIWFWMT 240
             WPCAPG+KY+GRGPIQLTHNYNYG AG+A+  +L+NNPDL++T+P VSF+TAIWFWMT
Sbjct: 181 --WPCAPGKKYYGRGPIQLTHNYNYGLAGQAIKEDLINNPDLLSTNPTVSFKTAIWFWMT 240

Query: 241 PQGNKPSSHDVIIGRWQPSNVDRAAGRAAGYGVITNIINGEFECGSGPNDRATSRIGFYT 300
           PQ NKPSSHDVI GRW PS  D +AGR  GYGVITNIING  ECG G ++R   R+GFY 
Sbjct: 241 PQANKPSSHDVITGRWTPSAADSSAGRVPGYGVITNIINGGIECGHGQDNRVDDRVGFYK 300

Query: 301 SYCNLLGVTMGPNLDCNNQRPF 319
            YC + GV  G NLDCNNQR F
Sbjct: 301 RYCQIFGVDPGGNLDCNNQRSF 319

BLAST of CmaCh07G003910 vs. ExPASy Swiss-Prot
Match: Q7DNA1 (Chitinase 2 OS=Oryza sativa subsp. japonica OX=39947 GN=Cht2 PE=1 SV=1)

HSP 1 Score: 448.4 bits (1152), Expect = 7.0e-125
Identity = 207/321 (64.49%), Postives = 242/321 (75.39%), Query Frame = 0

Query: 2   KRMKLHAFIFLSLALLLSASAQQCGRQANSAVCQNGLCCSEHGWCGTTDAYCGAGCQSQC 61
           K+  L A   L+ AL  +A A+QCG QA  A C N LCCS  GWCGTT  +CG GCQSQC
Sbjct: 12  KKAALVALAVLAAALATAARAEQCGAQAGGARCPNCLCCSRWGWCGTTSDFCGDGCQSQC 71

Query: 62  RRPGPTPT-LPPNAGSGVGRIITAAVFDQLLKYRNDPRCRSNGFYTYNAFLTAARSFPAF 121
              GPTPT  PP+   GVG I+   +F++LL +RND  C + GFYTY AFL AA +FPAF
Sbjct: 72  SGCGPTPTPTPPSPSDGVGSIVPRDLFERLLLHRNDGACPARGFYTYEAFLAAAAAFPAF 131

Query: 122 GTTGDDATRRRELAAFLAQTSHETTGGWPTAPDGPYAWGYCFVRELRQDA-YCRSPDPEW 181
           G TG+  TR+RE+AAFL QTSHETTGGWPTAPDGP++WGYCF +E    + YC+ P PEW
Sbjct: 132 GGTGNTETRKREVAAFLGQTSHETTGGWPTAPDGPFSWGYCFKQEQNPPSDYCQ-PSPEW 191

Query: 182 PCAPGQKYFGRGPIQLTHNYNYGQAGRALDLNLLNNPDLVATDPIVSFRTAIWFWMTPQG 241
           PCAPG+KY+GRGPIQL+ N+NYG AGRA+ ++LL+NPDLVATD  VSF+TA+WFWMTPQG
Sbjct: 192 PCAPGRKYYGRGPIQLSFNFNYGPAGRAIGVDLLSNPDLVATDATVSFKTALWFWMTPQG 251

Query: 242 NKPSSHDVIIGRWQPSNVDRAAGRAAGYGVITNIINGEFECGSGPNDRATSRIGFYTSYC 301
           NKPSSHDVI GRW PS  D AAGRA GYGVITNI+NG  ECG GP+DR  +RIGFY  YC
Sbjct: 252 NKPSSHDVITGRWAPSPADAAAGRAPGYGVITNIVNGGLECGHGPDDRVANRIGFYQRYC 311

Query: 302 NLLGVTMGPNLDCNNQRPFGS 321
              G+  G NLDC NQRPF S
Sbjct: 312 GAFGIGTGGNLDCYNQRPFNS 331

BLAST of CmaCh07G003910 vs. ExPASy Swiss-Prot
Match: P08252 (Endochitinase A OS=Nicotiana tabacum OX=4097 GN=CHN48 PE=1 SV=2)

HSP 1 Score: 447.6 bits (1150), Expect = 1.2e-124
Identity = 203/307 (66.12%), Postives = 241/307 (78.50%), Query Frame = 0

Query: 16  LLLSASAQQCGRQANSAVCQNGLCCSEHGWCGTTDAYCGAG-CQSQCRRPGPTPTLP-PN 75
           LLLSASA+QCG QA  A C +GLCCS+ GWCG T+ YCG G CQSQC   GPTPT P P 
Sbjct: 17  LLLSASAEQCGSQAGGARCPSGLCCSKFGWCGNTNDYCGPGNCQSQC-PGGPTPTPPTPP 76

Query: 76  AGSGVGRIITAAVFDQLLKYRNDPRCRSNGFYTYNAFLTAARSFPAFGTTGDDATRRREL 135
            G  +G II++++FDQ+LK+RND  C+  GFY+YNAF+ AARSFP FGT+GD   R+RE+
Sbjct: 77  GGGDLGSIISSSMFDQMLKHRNDNACQGKGFYSYNAFINAARSFPGFGTSGDTTARKREI 136

Query: 136 AAFLAQTSHETTGGWPTAPDGPYAWGYCFVRELRQDAYCRSPDPEWPCAPGQKYFGRGPI 195
           AAF AQTSHETTGGW TAPDGPYAWGYC++RE        +P  +WPCAPG+KYFGRGPI
Sbjct: 137 AAFFAQTSHETTGGWATAPDGPYAWGYCWLREQGSPGDYCTPSGQWPCAPGRKYFGRGPI 196

Query: 196 QLTHNYNYGQAGRALDLNLLNNPDLVATDPIVSFRTAIWFWMTPQGNKPSSHDVIIGRWQ 255
           Q++HNYNYG  GRA+ ++LLNNPDLVATDP++SF++A+WFWMTPQ  KPS HDVIIGRWQ
Sbjct: 197 QISHNYNYGPCGRAIGVDLLNNPDLVATDPVISFKSALWFWMTPQSPKPSCHDVIIGRWQ 256

Query: 256 PSNVDRAAGRAAGYGVITNIINGEFECGSGPNDRATSRIGFYTSYCNLLGVTMGPNLDCN 315
           PS  DRAA R  G+GVITNIING  ECG G + R   RIGFY  YC++LGV+ G NLDC 
Sbjct: 257 PSAGDRAANRLPGFGVITNIINGGLECGRGTDSRVQDRIGFYRRYCSILGVSPGDNLDCG 316

Query: 316 NQRPFGS 321
           NQR FG+
Sbjct: 317 NQRSFGN 322

BLAST of CmaCh07G003910 vs. ExPASy Swiss-Prot
Match: P24091 (Endochitinase B OS=Nicotiana tabacum OX=4097 GN=CHN50 PE=1 SV=1)

HSP 1 Score: 442.6 bits (1137), Expect = 3.9e-123
Identity = 205/322 (63.66%), Postives = 245/322 (76.09%), Query Frame = 0

Query: 4   MKLHAFIFLS----LALLLSASAQQCGRQANSAVCQNGLCCSEHGWCGTTDAYCGAG-CQ 63
           M+L  F  LS      LLLSASA+QCG QA  A C +GLCCS+ GWCG T+ YCG G CQ
Sbjct: 1   MRLREFTALSSLLFSLLLLSASAEQCGSQAGGARCASGLCCSKFGWCGNTNDYCGPGNCQ 60

Query: 64  SQCRRPGPTPTLPPNAGSGVGRIITAAVFDQLLKYRNDPRCRSNGFYTYNAFLTAARSFP 123
           SQC   GPTP      G  +G II++++FDQ+LK+RND  C+  GFY+YNAF+ AARSFP
Sbjct: 61  SQC-PGGPTPP----GGGDLGSIISSSMFDQMLKHRNDNACQGKGFYSYNAFINAARSFP 120

Query: 124 AFGTTGDDATRRRELAAFLAQTSHETTGGWPTAPDGPYAWGYCFVRELRQDAYCRSPDPE 183
            FGT+GD   R+RE+AAF AQTSHETTGGW TAPDGPYAWGYC++RE        +P  +
Sbjct: 121 GFGTSGDTTARKREIAAFFAQTSHETTGGWATAPDGPYAWGYCWLREQGSPGDYCTPSGQ 180

Query: 184 WPCAPGQKYFGRGPIQLTHNYNYGQAGRALDLNLLNNPDLVATDPIVSFRTAIWFWMTPQ 243
           WPCAPG+KYFGRGPIQ++HNYNYG  GRA+ ++LLNNPDLVATDP++SF++A+WFWMTPQ
Sbjct: 181 WPCAPGRKYFGRGPIQISHNYNYGPCGRAIGVDLLNNPDLVATDPVISFKSALWFWMTPQ 240

Query: 244 GNKPSSHDVIIGRWQPSNVDRAAGRAAGYGVITNIINGEFECGSGPNDRATSRIGFYTSY 303
             KPS HDVIIGRWQPS+ DRAA R  G+GVITNIING  ECG G + R   RIGFY  Y
Sbjct: 241 SPKPSCHDVIIGRWQPSSADRAANRLPGFGVITNIINGGLECGRGTDSRVQDRIGFYRRY 300

Query: 304 CNLLGVTMGPNLDCNNQRPFGS 321
           C++LGV+ G NLDC NQR FG+
Sbjct: 301 CSILGVSPGDNLDCGNQRSFGN 317

BLAST of CmaCh07G003910 vs. ExPASy Swiss-Prot
Match: Q05538 (Basic 30 kDa endochitinase OS=Solanum lycopersicum OX=4081 GN=CHI9 PE=1 SV=1)

HSP 1 Score: 436.0 bits (1120), Expect = 3.6e-121
Identity = 204/322 (63.35%), Postives = 241/322 (74.84%), Query Frame = 0

Query: 4   MKLHAFIFLSL---ALLLSASAQQCGRQANSAVCQNGLCCSEHGWCGTTDAYCGAG-CQS 63
           M+L  F  L L    LLLSASA+QCG QA  A+C +GLCCS+ GWCG T+ YCG G CQS
Sbjct: 1   MRLSEFTTLFLLFSVLLLSASAEQCGSQAGGALCASGLCCSKFGWCGNTNEYCGPGNCQS 60

Query: 64  QCRRPGPTPTLPPNAGSGVGRIITAAVFDQLLKYRNDPRCR-SNGFYTYNAFLTAARSFP 123
           QC  PG      P     +G +I+ ++FDQ+L +RND  C+  N FY+YNAF+TAA SFP
Sbjct: 61  QC--PG-----GPGPSGDLGGVISNSMFDQMLNHRNDNACQGKNNFYSYNAFVTAAGSFP 120

Query: 124 AFGTTGDDATRRRELAAFLAQTSHETTGGWPTAPDGPYAWGYCFVRELRQDAYCRSPDPE 183
            FGTTGD   R+RE+AAFLAQTSHETTGGWPTAPDGPYAWGYCF+RE        +P  +
Sbjct: 121 GFGTTGDITARKREIAAFLAQTSHETTGGWPTAPDGPYAWGYCFLREQGSPGDYCTPSSQ 180

Query: 184 WPCAPGQKYFGRGPIQLTHNYNYGQAGRALDLNLLNNPDLVATDPIVSFRTAIWFWMTPQ 243
           WPCAPG+KYFGRGPIQ++HNYNYG  GRA+ ++LLNNPDLVATDP++SF++AIWFWMTPQ
Sbjct: 181 WPCAPGRKYFGRGPIQISHNYNYGPCGRAIGVDLLNNPDLVATDPVISFKSAIWFWMTPQ 240

Query: 244 GNKPSSHDVIIGRWQPSNVDRAAGRAAGYGVITNIINGEFECGSGPNDRATSRIGFYTSY 303
             KPS HDVI GRWQPS  D+AA R  G+GVITNIING  ECG G + R   RIGFY  Y
Sbjct: 241 SPKPSCHDVITGRWQPSGADQAANRVPGFGVITNIINGGLECGHGSDSRVQDRIGFYRRY 300

Query: 304 CNLLGVTMGPNLDCNNQRPFGS 321
           C +LGV+ G NLDC NQR FG+
Sbjct: 301 CGILGVSPGENLDCGNQRSFGN 315

BLAST of CmaCh07G003910 vs. ExPASy TrEMBL
Match: A0A6J1KUS6 (endochitinase-like OS=Cucurbita maxima OX=3661 GN=LOC111497387 PE=4 SV=1)

HSP 1 Score: 687.2 bits (1772), Expect = 3.3e-194
Identity = 318/318 (100.00%), Postives = 318/318 (100.00%), Query Frame = 0

Query: 4   MKLHAFIFLSLALLLSASAQQCGRQANSAVCQNGLCCSEHGWCGTTDAYCGAGCQSQCRR 63
           MKLHAFIFLSLALLLSASAQQCGRQANSAVCQNGLCCSEHGWCGTTDAYCGAGCQSQCRR
Sbjct: 1   MKLHAFIFLSLALLLSASAQQCGRQANSAVCQNGLCCSEHGWCGTTDAYCGAGCQSQCRR 60

Query: 64  PGPTPTLPPNAGSGVGRIITAAVFDQLLKYRNDPRCRSNGFYTYNAFLTAARSFPAFGTT 123
           PGPTPTLPPNAGSGVGRIITAAVFDQLLKYRNDPRCRSNGFYTYNAFLTAARSFPAFGTT
Sbjct: 61  PGPTPTLPPNAGSGVGRIITAAVFDQLLKYRNDPRCRSNGFYTYNAFLTAARSFPAFGTT 120

Query: 124 GDDATRRRELAAFLAQTSHETTGGWPTAPDGPYAWGYCFVRELRQDAYCRSPDPEWPCAP 183
           GDDATRRRELAAFLAQTSHETTGGWPTAPDGPYAWGYCFVRELRQDAYCRSPDPEWPCAP
Sbjct: 121 GDDATRRRELAAFLAQTSHETTGGWPTAPDGPYAWGYCFVRELRQDAYCRSPDPEWPCAP 180

Query: 184 GQKYFGRGPIQLTHNYNYGQAGRALDLNLLNNPDLVATDPIVSFRTAIWFWMTPQGNKPS 243
           GQKYFGRGPIQLTHNYNYGQAGRALDLNLLNNPDLVATDPIVSFRTAIWFWMTPQGNKPS
Sbjct: 181 GQKYFGRGPIQLTHNYNYGQAGRALDLNLLNNPDLVATDPIVSFRTAIWFWMTPQGNKPS 240

Query: 244 SHDVIIGRWQPSNVDRAAGRAAGYGVITNIINGEFECGSGPNDRATSRIGFYTSYCNLLG 303
           SHDVIIGRWQPSNVDRAAGRAAGYGVITNIINGEFECGSGPNDRATSRIGFYTSYCNLLG
Sbjct: 241 SHDVIIGRWQPSNVDRAAGRAAGYGVITNIINGEFECGSGPNDRATSRIGFYTSYCNLLG 300

Query: 304 VTMGPNLDCNNQRPFGSR 322
           VTMGPNLDCNNQRPFGSR
Sbjct: 301 VTMGPNLDCNNQRPFGSR 318

BLAST of CmaCh07G003910 vs. ExPASy TrEMBL
Match: A0A6J1EJE5 (endochitinase-like OS=Cucurbita moschata OX=3662 GN=LOC111433851 PE=4 SV=1)

HSP 1 Score: 662.1 bits (1707), Expect = 1.1e-186
Identity = 306/316 (96.84%), Postives = 308/316 (97.47%), Query Frame = 0

Query: 4   MKLHAFIFLSLALLLSASAQQCGRQANSAVCQNGLCCSEHGWCGTTDAYCGAGCQSQCRR 63
           MKLHAFIFLSLALLLSASAQQCGRQANSAVCQNGLCCSEHGWCGTTDAYCGAGCQSQCRR
Sbjct: 1   MKLHAFIFLSLALLLSASAQQCGRQANSAVCQNGLCCSEHGWCGTTDAYCGAGCQSQCRR 60

Query: 64  PGPTPTLPPNAGSGVGRIITAAVFDQLLKYRNDPRCRSNGFYTYNAFLTAARSFPAFGTT 123
           PGPTPTLPPNAGSGVG IITAAVFD+LLKYRNDPRCRSNGFYTYNAFL AARSFPAFGTT
Sbjct: 61  PGPTPTLPPNAGSGVGGIITAAVFDRLLKYRNDPRCRSNGFYTYNAFLAAARSFPAFGTT 120

Query: 124 GDDATRRRELAAFLAQTSHETTGGWPTAPDGPYAWGYCFVRELRQDAYCRSPDPEWPCAP 183
           GDDATRRRELAAFLAQTSHETTGGWPTAPDGPYAWGYCFVREL+QDAYCRSPDPEWPCAP
Sbjct: 121 GDDATRRRELAAFLAQTSHETTGGWPTAPDGPYAWGYCFVRELQQDAYCRSPDPEWPCAP 180

Query: 184 GQKYFGRGPIQLTHNYNYGQAGRALDLNLLNNPDLVATDPIVSFRTAIWFWMTPQGNKPS 243
           GQKYFGRGPIQLTHNYNYGQAGRAL LNLLNNPDLVATDPIVSFRTAIWFWMTPQGNKPS
Sbjct: 181 GQKYFGRGPIQLTHNYNYGQAGRALGLNLLNNPDLVATDPIVSFRTAIWFWMTPQGNKPS 240

Query: 244 SHDVIIGRWQPSNVDRAAGRAAGYGVITNIINGEFECGSGPNDRATSRIGFYTSYCNLLG 303
           SHDVIIGRWQPSNVDRAAGRAAGYGVITNIING  ECG GPNDR TSRIGFYT YCNLLG
Sbjct: 241 SHDVIIGRWQPSNVDRAAGRAAGYGVITNIINGGLECGRGPNDRMTSRIGFYTRYCNLLG 300

Query: 304 VTMGPNLDCNNQRPFG 320
           VTMGPNLDCNNQRPFG
Sbjct: 301 VTMGPNLDCNNQRPFG 316

BLAST of CmaCh07G003910 vs. ExPASy TrEMBL
Match: A0A6J1BU42 (endochitinase A-like OS=Momordica charantia OX=3673 GN=LOC111005566 PE=4 SV=1)

HSP 1 Score: 516.9 bits (1330), Expect = 5.9e-143
Identity = 238/319 (74.61%), Postives = 264/319 (82.76%), Query Frame = 0

Query: 2   KRMKLHAFIFLSLALLL-SASAQQCGRQANSAVCQNGLCCSEHGWCGTTDAYCGAGCQSQ 61
           K+MK H  I L+ AL L +ASA+QCGRQAN A+C N LCCS+HGWCG+TD YC  GCQSQ
Sbjct: 315 KKMKAHTLIILAFALFLGAASAEQCGRQANGALCPNRLCCSQHGWCGSTDEYCKNGCQSQ 374

Query: 62  CRRPGPTPTLPPNAGSG-VGRIITAAVFDQLLKYRNDPRCRSNGFYTYNAFLTAARSFPA 121
           C    PTPT   N GSG VGRIIT A+FDQ+LKYRND RC SNGFY+YNAF++A RSFP 
Sbjct: 375 CGGQTPTPT---NPGSGDVGRIITPAIFDQMLKYRNDARCPSNGFYSYNAFISATRSFPG 434

Query: 122 FGTTGDDATRRRELAAFLAQTSHETTGGWPTAPDGPYAWGYCFVRELRQDAYCRSPDPEW 181
           FGTTGDDATR+RELAAFL QTSHETTGGWP+APDGP+AWGYCF+RE  QD YC SP+ +W
Sbjct: 435 FGTTGDDATRKRELAAFLGQTSHETTGGWPSAPDGPFAWGYCFIRERNQDTYC-SPNQQW 494

Query: 182 PCAPGQKYFGRGPIQLTHNYNYGQAGRALDLNLLNNPDLVATDPIVSFRTAIWFWMTPQG 241
           PCAPGQKY+GRGPIQLTHNYNYG AGRAL LNLLNNPD VATDP VSF+TAIWFWMTPQG
Sbjct: 495 PCAPGQKYYGRGPIQLTHNYNYGPAGRALGLNLLNNPDSVATDPTVSFKTAIWFWMTPQG 554

Query: 242 NKPSSHDVIIGRWQPSNVDRAAGRAAGYGVITNIINGEFECGSGPNDRATSRIGFYTSYC 301
           NKPSSHDVIIG+WQPS  D AAGR  GYGVITNIING  ECG GP+ R   RIGF+  YC
Sbjct: 555 NKPSSHDVIIGKWQPSGADSAAGRVPGYGVITNIINGGLECGRGPDSRVADRIGFFKRYC 614

Query: 302 NLLGVTMGPNLDCNNQRPF 319
           ++L +  G NLDCNNQRPF
Sbjct: 615 DILRIGYGNNLDCNNQRPF 629

BLAST of CmaCh07G003910 vs. ExPASy TrEMBL
Match: Q207U1 (Chitinase OS=Momordica charantia OX=3673 GN=ChiA PE=4 SV=1)

HSP 1 Score: 514.2 bits (1323), Expect = 3.8e-142
Identity = 237/317 (74.76%), Postives = 262/317 (82.65%), Query Frame = 0

Query: 4   MKLHAFIFLSLALLL-SASAQQCGRQANSAVCQNGLCCSEHGWCGTTDAYCGAGCQSQCR 63
           MK H  I L+ AL L +ASA+QCGRQAN A+C N LCCS+HGWCG+TD YC  GCQSQC 
Sbjct: 1   MKAHTLIILAFALFLGAASAEQCGRQANGALCPNRLCCSQHGWCGSTDEYCKNGCQSQCG 60

Query: 64  RPGPTPTLPPNAGSG-VGRIITAAVFDQLLKYRNDPRCRSNGFYTYNAFLTAARSFPAFG 123
              PTPT   N GSG VGRIIT A+FDQ+LKYRND RC SNGFY+YNAF++A RSFP FG
Sbjct: 61  GQTPTPT---NPGSGDVGRIITPAIFDQMLKYRNDARCPSNGFYSYNAFISATRSFPGFG 120

Query: 124 TTGDDATRRRELAAFLAQTSHETTGGWPTAPDGPYAWGYCFVRELRQDAYCRSPDPEWPC 183
           TTGDDATR+RELAAFL QTSHETTGGWP+APDGP+AWGYCF+RE  QD YC SP+ +WPC
Sbjct: 121 TTGDDATRKRELAAFLGQTSHETTGGWPSAPDGPFAWGYCFIRERNQDTYC-SPNQQWPC 180

Query: 184 APGQKYFGRGPIQLTHNYNYGQAGRALDLNLLNNPDLVATDPIVSFRTAIWFWMTPQGNK 243
           APGQKY+GRGPIQLTHNYNYG AGRAL LNLLNNPD VATDP VSF+TAIWFWMTPQGNK
Sbjct: 181 APGQKYYGRGPIQLTHNYNYGPAGRALGLNLLNNPDSVATDPTVSFKTAIWFWMTPQGNK 240

Query: 244 PSSHDVIIGRWQPSNVDRAAGRAAGYGVITNIINGEFECGSGPNDRATSRIGFYTSYCNL 303
           PSSHDVIIG+WQPS  D AAGR  GYGVITNIING  ECG GP+ R   RIGF+  YC++
Sbjct: 241 PSSHDVIIGKWQPSGADSAAGRVPGYGVITNIINGGLECGRGPDSRVADRIGFFKRYCDI 300

Query: 304 LGVTMGPNLDCNNQRPF 319
           L +  G NLDCNNQRPF
Sbjct: 301 LRIGYGNNLDCNNQRPF 313

BLAST of CmaCh07G003910 vs. ExPASy TrEMBL
Match: A0A6J1GD14 (endochitinase-like OS=Cucurbita moschata OX=3662 GN=LOC111453081 PE=4 SV=1)

HSP 1 Score: 497.7 bits (1280), Expect = 3.7e-137
Identity = 227/316 (71.84%), Postives = 257/316 (81.33%), Query Frame = 0

Query: 4   MKLHAFIFLSLALLLSASAQQCGRQANSAVCQNGLCCSEHGWCGTTDAYCGAGCQSQCRR 63
           MKLH  I L+ A +L  +A+QCGRQAN A+C NGLCCS+ GWCG TD YC  GCQSQC  
Sbjct: 8   MKLHTLIVLAFAFVLGGAAEQCGRQANGALCPNGLCCSQFGWCGDTDEYCKDGCQSQC-- 67

Query: 64  PGPTPTLPPNA-GSGVGRIITAAVFDQLLKYRNDPRCRSNGFYTYNAFLTAARSFPAFGT 123
            G TP+ PP++ GSGVG II+ ++++Q+LKY  DPRC SNGFY YNAF+TAA+SFP FGT
Sbjct: 68  GGSTPSTPPSSGGSGVGSIISESLYNQMLKYSKDPRCPSNGFYRYNAFVTAAQSFPGFGT 127

Query: 124 TGDDATRRRELAAFLAQTSHETTGGWPTAPDGPYAWGYCFVRELRQDAYCRSPDPEWPCA 183
           TGD ATR+RELAAF  QTSHETTGGW TAPDGPYAWGYCF+RE  QD YC SP+ +WPCA
Sbjct: 128 TGDAATRKRELAAFFGQTSHETTGGWATAPDGPYAWGYCFIRERNQDVYC-SPNQQWPCA 187

Query: 184 PGQKYFGRGPIQLTHNYNYGQAGRALDLNLLNNPDLVATDPIVSFRTAIWFWMTPQGNKP 243
            GQKY+GRGPIQLTHNYNYG AG AL+LNLL NPDLVATD +VSF+TAIWFWMTPQGNKP
Sbjct: 188 AGQKYYGRGPIQLTHNYNYGPAGNALNLNLLGNPDLVATDAVVSFKTAIWFWMTPQGNKP 247

Query: 244 SSHDVIIGRWQPSNVDRAAGRAAGYGVITNIINGEFECGSGPNDRATSRIGFYTSYCNLL 303
           S HDVI GRWQPS+ D AAGR  GYGVITNIING  ECG GP+DR   RIGFY  Y ++L
Sbjct: 248 SCHDVITGRWQPSSADSAAGRVPGYGVITNIINGGLECGHGPDDRVADRIGFYKRYSDML 307

Query: 304 GVTMGPNLDCNNQRPF 319
           GV+ G NLDCNNQR F
Sbjct: 308 GVSYGSNLDCNNQRSF 320

BLAST of CmaCh07G003910 vs. NCBI nr
Match: XP_023003944.1 (endochitinase-like [Cucurbita maxima])

HSP 1 Score: 687.2 bits (1772), Expect = 6.9e-194
Identity = 318/318 (100.00%), Postives = 318/318 (100.00%), Query Frame = 0

Query: 4   MKLHAFIFLSLALLLSASAQQCGRQANSAVCQNGLCCSEHGWCGTTDAYCGAGCQSQCRR 63
           MKLHAFIFLSLALLLSASAQQCGRQANSAVCQNGLCCSEHGWCGTTDAYCGAGCQSQCRR
Sbjct: 1   MKLHAFIFLSLALLLSASAQQCGRQANSAVCQNGLCCSEHGWCGTTDAYCGAGCQSQCRR 60

Query: 64  PGPTPTLPPNAGSGVGRIITAAVFDQLLKYRNDPRCRSNGFYTYNAFLTAARSFPAFGTT 123
           PGPTPTLPPNAGSGVGRIITAAVFDQLLKYRNDPRCRSNGFYTYNAFLTAARSFPAFGTT
Sbjct: 61  PGPTPTLPPNAGSGVGRIITAAVFDQLLKYRNDPRCRSNGFYTYNAFLTAARSFPAFGTT 120

Query: 124 GDDATRRRELAAFLAQTSHETTGGWPTAPDGPYAWGYCFVRELRQDAYCRSPDPEWPCAP 183
           GDDATRRRELAAFLAQTSHETTGGWPTAPDGPYAWGYCFVRELRQDAYCRSPDPEWPCAP
Sbjct: 121 GDDATRRRELAAFLAQTSHETTGGWPTAPDGPYAWGYCFVRELRQDAYCRSPDPEWPCAP 180

Query: 184 GQKYFGRGPIQLTHNYNYGQAGRALDLNLLNNPDLVATDPIVSFRTAIWFWMTPQGNKPS 243
           GQKYFGRGPIQLTHNYNYGQAGRALDLNLLNNPDLVATDPIVSFRTAIWFWMTPQGNKPS
Sbjct: 181 GQKYFGRGPIQLTHNYNYGQAGRALDLNLLNNPDLVATDPIVSFRTAIWFWMTPQGNKPS 240

Query: 244 SHDVIIGRWQPSNVDRAAGRAAGYGVITNIINGEFECGSGPNDRATSRIGFYTSYCNLLG 303
           SHDVIIGRWQPSNVDRAAGRAAGYGVITNIINGEFECGSGPNDRATSRIGFYTSYCNLLG
Sbjct: 241 SHDVIIGRWQPSNVDRAAGRAAGYGVITNIINGEFECGSGPNDRATSRIGFYTSYCNLLG 300

Query: 304 VTMGPNLDCNNQRPFGSR 322
           VTMGPNLDCNNQRPFGSR
Sbjct: 301 VTMGPNLDCNNQRPFGSR 318

BLAST of CmaCh07G003910 vs. NCBI nr
Match: KAG6594739.1 (hypothetical protein SDJN03_11292, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 665.2 bits (1715), Expect = 2.8e-187
Identity = 307/316 (97.15%), Postives = 311/316 (98.42%), Query Frame = 0

Query: 4   MKLHAFIFLSLALLLSASAQQCGRQANSAVCQNGLCCSEHGWCGTTDAYCGAGCQSQCRR 63
           MKLHAFIFLSLALLLSASAQQCGRQANSAVCQNGLCCSEHGWCGTTDAYCGAGCQSQCRR
Sbjct: 1   MKLHAFIFLSLALLLSASAQQCGRQANSAVCQNGLCCSEHGWCGTTDAYCGAGCQSQCRR 60

Query: 64  PGPTPTLPPNAGSGVGRIITAAVFDQLLKYRNDPRCRSNGFYTYNAFLTAARSFPAFGTT 123
           PGPTPTLPPNAGSGVG IITAAVFD+LLKYRNDPRCRSNGFYTYNAFLTAARSFPAFG+T
Sbjct: 61  PGPTPTLPPNAGSGVGGIITAAVFDRLLKYRNDPRCRSNGFYTYNAFLTAARSFPAFGST 120

Query: 124 GDDATRRRELAAFLAQTSHETTGGWPTAPDGPYAWGYCFVRELRQDAYCRSPDPEWPCAP 183
           GDDATRRRELAAFLAQTSHETTGGWPTAPDGPYAWGYCFVREL+QDAYCRSPDPEWPCAP
Sbjct: 121 GDDATRRRELAAFLAQTSHETTGGWPTAPDGPYAWGYCFVRELQQDAYCRSPDPEWPCAP 180

Query: 184 GQKYFGRGPIQLTHNYNYGQAGRALDLNLLNNPDLVATDPIVSFRTAIWFWMTPQGNKPS 243
           GQKYFGRGPIQLTHNYNYGQAGRAL+LNLLNNPDLVATDPIVSFRTAIWFWMTPQGNKPS
Sbjct: 181 GQKYFGRGPIQLTHNYNYGQAGRALNLNLLNNPDLVATDPIVSFRTAIWFWMTPQGNKPS 240

Query: 244 SHDVIIGRWQPSNVDRAAGRAAGYGVITNIINGEFECGSGPNDRATSRIGFYTSYCNLLG 303
           SHDVIIGRWQPSNVDRAAGRAAGYGVITNIING  ECGSGPNDR TSRIGFYT YCNLLG
Sbjct: 241 SHDVIIGRWQPSNVDRAAGRAAGYGVITNIINGGLECGSGPNDRMTSRIGFYTRYCNLLG 300

Query: 304 VTMGPNLDCNNQRPFG 320
           VTMGPNLDCNNQRPFG
Sbjct: 301 VTMGPNLDCNNQRPFG 316

BLAST of CmaCh07G003910 vs. NCBI nr
Match: XP_022926868.1 (endochitinase-like [Cucurbita moschata])

HSP 1 Score: 662.1 bits (1707), Expect = 2.4e-186
Identity = 306/316 (96.84%), Postives = 308/316 (97.47%), Query Frame = 0

Query: 4   MKLHAFIFLSLALLLSASAQQCGRQANSAVCQNGLCCSEHGWCGTTDAYCGAGCQSQCRR 63
           MKLHAFIFLSLALLLSASAQQCGRQANSAVCQNGLCCSEHGWCGTTDAYCGAGCQSQCRR
Sbjct: 1   MKLHAFIFLSLALLLSASAQQCGRQANSAVCQNGLCCSEHGWCGTTDAYCGAGCQSQCRR 60

Query: 64  PGPTPTLPPNAGSGVGRIITAAVFDQLLKYRNDPRCRSNGFYTYNAFLTAARSFPAFGTT 123
           PGPTPTLPPNAGSGVG IITAAVFD+LLKYRNDPRCRSNGFYTYNAFL AARSFPAFGTT
Sbjct: 61  PGPTPTLPPNAGSGVGGIITAAVFDRLLKYRNDPRCRSNGFYTYNAFLAAARSFPAFGTT 120

Query: 124 GDDATRRRELAAFLAQTSHETTGGWPTAPDGPYAWGYCFVRELRQDAYCRSPDPEWPCAP 183
           GDDATRRRELAAFLAQTSHETTGGWPTAPDGPYAWGYCFVREL+QDAYCRSPDPEWPCAP
Sbjct: 121 GDDATRRRELAAFLAQTSHETTGGWPTAPDGPYAWGYCFVRELQQDAYCRSPDPEWPCAP 180

Query: 184 GQKYFGRGPIQLTHNYNYGQAGRALDLNLLNNPDLVATDPIVSFRTAIWFWMTPQGNKPS 243
           GQKYFGRGPIQLTHNYNYGQAGRAL LNLLNNPDLVATDPIVSFRTAIWFWMTPQGNKPS
Sbjct: 181 GQKYFGRGPIQLTHNYNYGQAGRALGLNLLNNPDLVATDPIVSFRTAIWFWMTPQGNKPS 240

Query: 244 SHDVIIGRWQPSNVDRAAGRAAGYGVITNIINGEFECGSGPNDRATSRIGFYTSYCNLLG 303
           SHDVIIGRWQPSNVDRAAGRAAGYGVITNIING  ECG GPNDR TSRIGFYT YCNLLG
Sbjct: 241 SHDVIIGRWQPSNVDRAAGRAAGYGVITNIINGGLECGRGPNDRMTSRIGFYTRYCNLLG 300

Query: 304 VTMGPNLDCNNQRPFG 320
           VTMGPNLDCNNQRPFG
Sbjct: 301 VTMGPNLDCNNQRPFG 316

BLAST of CmaCh07G003910 vs. NCBI nr
Match: XP_023518615.1 (endochitinase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 654.1 bits (1686), Expect = 6.4e-184
Identity = 305/320 (95.31%), Postives = 308/320 (96.25%), Query Frame = 0

Query: 1   MKRMKLHAFIFLSLALLLSASAQQCGRQANSAVCQNGLCCSEHGWCGTTDAYCGAGCQSQ 60
           MK MKLHAFIFLSLALLLSASAQQCGRQANSAVCQNGLCCSEHGWCG TDAYCGAGCQSQ
Sbjct: 1   MKNMKLHAFIFLSLALLLSASAQQCGRQANSAVCQNGLCCSEHGWCGNTDAYCGAGCQSQ 60

Query: 61  CRRPGPTPTLPPN-AGSGVGRIITAAVFDQLLKYRNDPRCRSNGFYTYNAFLTAARSFPA 120
           CRRPGPTPTLPPN AGSGVG IITAAVFD+LLKYRNDPRCRSNGFYTYNAFLTAARSFPA
Sbjct: 61  CRRPGPTPTLPPNDAGSGVGGIITAAVFDRLLKYRNDPRCRSNGFYTYNAFLTAARSFPA 120

Query: 121 FGTTGDDATRRRELAAFLAQTSHETTGGWPTAPDGPYAWGYCFVRELRQDAYCRSPDPEW 180
           FGTTGD  TRRRELAAFLAQTSHETTGGWPTAPDGPYAWGYCFVREL+QDAYCRS DPEW
Sbjct: 121 FGTTGDVTTRRRELAAFLAQTSHETTGGWPTAPDGPYAWGYCFVRELQQDAYCRSHDPEW 180

Query: 181 PCAPGQKYFGRGPIQLTHNYNYGQAGRALDLNLLNNPDLVATDPIVSFRTAIWFWMTPQG 240
           PCAPGQKYFGRGPIQLTHNYNYGQAGRAL+LNLLNNPDLVAT PIVSFRTAIWFWMTPQG
Sbjct: 181 PCAPGQKYFGRGPIQLTHNYNYGQAGRALNLNLLNNPDLVATHPIVSFRTAIWFWMTPQG 240

Query: 241 NKPSSHDVIIGRWQPSNVDRAAGRAAGYGVITNIINGEFECGSGPNDRATSRIGFYTSYC 300
           NKPSSHDVIIGRWQPSNVDRAAGRAAGYGVITNIING  ECGSGPNDR TSRIGFYT YC
Sbjct: 241 NKPSSHDVIIGRWQPSNVDRAAGRAAGYGVITNIINGRLECGSGPNDRTTSRIGFYTRYC 300

Query: 301 NLLGVTMGPNLDCNNQRPFG 320
           NLLGVTMGPNLDCNNQRPFG
Sbjct: 301 NLLGVTMGPNLDCNNQRPFG 320

BLAST of CmaCh07G003910 vs. NCBI nr
Match: XP_022132794.1 (endochitinase A-like [Momordica charantia])

HSP 1 Score: 516.9 bits (1330), Expect = 1.2e-142
Identity = 238/319 (74.61%), Postives = 264/319 (82.76%), Query Frame = 0

Query: 2   KRMKLHAFIFLSLALLL-SASAQQCGRQANSAVCQNGLCCSEHGWCGTTDAYCGAGCQSQ 61
           K+MK H  I L+ AL L +ASA+QCGRQAN A+C N LCCS+HGWCG+TD YC  GCQSQ
Sbjct: 315 KKMKAHTLIILAFALFLGAASAEQCGRQANGALCPNRLCCSQHGWCGSTDEYCKNGCQSQ 374

Query: 62  CRRPGPTPTLPPNAGSG-VGRIITAAVFDQLLKYRNDPRCRSNGFYTYNAFLTAARSFPA 121
           C    PTPT   N GSG VGRIIT A+FDQ+LKYRND RC SNGFY+YNAF++A RSFP 
Sbjct: 375 CGGQTPTPT---NPGSGDVGRIITPAIFDQMLKYRNDARCPSNGFYSYNAFISATRSFPG 434

Query: 122 FGTTGDDATRRRELAAFLAQTSHETTGGWPTAPDGPYAWGYCFVRELRQDAYCRSPDPEW 181
           FGTTGDDATR+RELAAFL QTSHETTGGWP+APDGP+AWGYCF+RE  QD YC SP+ +W
Sbjct: 435 FGTTGDDATRKRELAAFLGQTSHETTGGWPSAPDGPFAWGYCFIRERNQDTYC-SPNQQW 494

Query: 182 PCAPGQKYFGRGPIQLTHNYNYGQAGRALDLNLLNNPDLVATDPIVSFRTAIWFWMTPQG 241
           PCAPGQKY+GRGPIQLTHNYNYG AGRAL LNLLNNPD VATDP VSF+TAIWFWMTPQG
Sbjct: 495 PCAPGQKYYGRGPIQLTHNYNYGPAGRALGLNLLNNPDSVATDPTVSFKTAIWFWMTPQG 554

Query: 242 NKPSSHDVIIGRWQPSNVDRAAGRAAGYGVITNIINGEFECGSGPNDRATSRIGFYTSYC 301
           NKPSSHDVIIG+WQPS  D AAGR  GYGVITNIING  ECG GP+ R   RIGF+  YC
Sbjct: 555 NKPSSHDVIIGKWQPSGADSAAGRVPGYGVITNIINGGLECGRGPDSRVADRIGFFKRYC 614

Query: 302 NLLGVTMGPNLDCNNQRPF 319
           ++L +  G NLDCNNQRPF
Sbjct: 615 DILRIGYGNNLDCNNQRPF 629

BLAST of CmaCh07G003910 vs. TAIR 10
Match: AT3G12500.1 (basic chitinase )

HSP 1 Score: 422.9 bits (1086), Expect = 2.2e-118
Identity = 198/320 (61.88%), Postives = 237/320 (74.06%), Query Frame = 0

Query: 1   MKRMKLHAFIFLSLALLLS-ASAQQCGRQANSAVCQNGLCCSEHGWCGTTDAYC-GAGCQ 60
           + +MK + F+FL  +LLLS +SA+QCGRQA  A+C NGLCCSE GWCG T+ YC   GCQ
Sbjct: 11  LNKMKTNLFLFLIFSLLLSLSSAEQCGRQAGGALCPNGLCCSEFGWCGNTEPYCKQPGCQ 70

Query: 61  SQCRRPGPTPTLPPNAGSGVGRIITAAVFDQLLKYRNDPRCRSNGFYTYNAFLTAARSFP 120
           SQC  PG TP  P    SG   II+++ FD +LK+RND  C + GFYTYNAF+TAA+SFP
Sbjct: 71  SQC-TPGGTPPGPTGDLSG---IISSSQFDDMLKHRNDAACPARGFYTYNAFITAAKSFP 130

Query: 121 AFGTTGDDATRRRELAAFLAQTSHETTGGWPTAPDGPYAWGYCFVRELRQDAYCRSPDPE 180
            FGTTGD ATR++E+AAF  QTSHETTGGW TAPDGPY+WGYCF +E    +    P   
Sbjct: 131 GFGTTGDTATRKKEVAAFFGQTSHETTGGWATAPDGPYSWGYCFKQEQNPASDYCEPSAT 190

Query: 181 WPCAPGQKYFGRGPIQLTHNYNYGQAGRALDLNLLNNPDLVATDPIVSFRTAIWFWMTPQ 240
           WPCA G++Y+GRGP+QL+ NYNYG  GRA+ ++LLNNPDLVA D +++F+ AIWFWMT Q
Sbjct: 191 WPCASGKRYYGRGPMQLSWNYNYGLCGRAIGVDLLNNPDLVANDAVIAFKAAIWFWMTAQ 250

Query: 241 GNKPSSHDVIIGRWQPSNVDRAAGRAAGYGVITNIINGEFECGSGPNDRATSRIGFYTSY 300
             KPS H VI G+WQPS+ DRAAGR  GYGVITNIING  ECG G + R   RIGFY  Y
Sbjct: 251 PPKPSCHAVIAGQWQPSDADRAAGRLPGYGVITNIINGGLECGRGQDGRVADRIGFYQRY 310

Query: 301 CNLLGVTMGPNLDCNNQRPF 319
           CN+ GV  G NLDC NQR F
Sbjct: 311 CNIFGVNPGGNLDCYNQRSF 326

BLAST of CmaCh07G003910 vs. TAIR 10
Match: AT4G01700.1 (Chitinase family protein )

HSP 1 Score: 306.2 bits (783), Expect = 3.1e-83
Identity = 133/239 (55.65%), Postives = 173/239 (72.38%), Query Frame = 0

Query: 81  IITAAVFDQLLKYRNDPRCRSNGFYTYNAFLTAARSFPAFGTTGDDATRRRELAAFLAQT 140
           ++   ++DQ+  ++++  C + GFY Y AF+ A RSFP FG+ G+  TRRRE+AAFLAQ 
Sbjct: 41  LVPRTLYDQIFIHKDNNACPAKGFYPYEAFVEATRSFPKFGSVGNFWTRRREVAAFLAQI 100

Query: 141 SHETTGGWPTAPDGPYAWGYCFVRELR-QDAYCRSPDPEWPCAPGQKYFGRGPIQLTHNY 200
           SHETTGGW TAPDGPYAWG CF  E+  Q  YC + + +WPC  G+ Y GRGPIQL+ NY
Sbjct: 101 SHETTGGWATAPDGPYAWGLCFKEEVSPQSNYCDASNKDWPCVSGKSYKGRGPIQLSWNY 160

Query: 201 NYGQAGRALDLNLLNNPDLVATDPIVSFRTAIWFWMTPQGNKPSSHDVIIGRWQPSNVDR 260
           NYGQAGRAL  + L NP+LVA + +++F+TA+WFWMT Q  KPS H+V++ R++P+  DR
Sbjct: 161 NYGQAGRALGFDGLQNPELVANNSVLAFKTALWFWMTEQTPKPSCHNVMVNRYRPTKADR 220

Query: 261 AAGRAAGYGVITNIINGEFECGSGPNDRATSRIGFYTSYCNLLGVTMGPNLDCNNQRPF 319
           AA R  GYG++TNIING  ECG   + R T R+G++  Y  L  VT GPNLDC NQRPF
Sbjct: 221 AANRTVGYGLVTNIINGGLECGIPGDGRVTDRVGYFQRYAQLFKVTTGPNLDCENQRPF 279

BLAST of CmaCh07G003910 vs. TAIR 10
Match: AT1G02360.1 (Chitinase family protein )

HSP 1 Score: 299.3 bits (765), Expect = 3.7e-81
Identity = 129/244 (52.87%), Postives = 171/244 (70.08%), Query Frame = 0

Query: 76  SGVGRIITAAVFDQLLKYRNDPRCRSNGFYTYNAFLTAARSFPAFGTTGDDATRRRELAA 135
           + + R++   +++++  ++++  C +NGFYTY +F+ A R FP FG+ G   T+R E+AA
Sbjct: 28  TSIERLVPRDLYNKIFIHKDNTACPANGFYTYESFVQATRRFPRFGSVGSPVTQRLEVAA 87

Query: 136 FLAQTSHETTGGWPTAPDGPYAWGYCFVRELR-QDAYCRSPDPEWPCAPGQKYFGRGPIQ 195
           FLAQ SHETTGGW TAPDGPYAWG CF  E+  Q  YC S D +WPC P + Y GRGPIQ
Sbjct: 88  FLAQISHETTGGWATAPDGPYAWGLCFKEEVSPQSTYCDSSDTQWPCFPNKTYQGRGPIQ 147

Query: 196 LTHNYNYGQAGRALDLNLLNNPDLVATDPIVSFRTAIWFWMTPQGNKPSSHDVIIGRWQP 255
           L+ NYNYG AGRAL  + L NP+ V+ + +++F+TA+WFWMTPQ  KPS HDV+IG+++P
Sbjct: 148 LSWNYNYGPAGRALGFDGLRNPETVSNNSVIAFQTALWFWMTPQSPKPSCHDVMIGKYRP 207

Query: 256 SNVDRAAGRAAGYGVITNIINGEFECGSGPNDRATSRIGFYTSYCNLLGVTMGPNLDCNN 315
           +  D AA R  G+G+ TNIING  ECG   + R   RIGF+  Y  L  V  GPNLDC N
Sbjct: 208 TAADLAANRTGGFGLTTNIINGGLECGIPGDGRVNDRIGFFQRYTGLFKVATGPNLDCEN 267

Query: 316 QRPF 319
           QRP+
Sbjct: 268 QRPY 271

BLAST of CmaCh07G003910 vs. TAIR 10
Match: AT2G43590.1 (Chitinase family protein )

HSP 1 Score: 206.8 bits (525), Expect = 2.5e-53
Identity = 120/315 (38.10%), Postives = 162/315 (51.43%), Query Frame = 0

Query: 8   AFIFLSLALLL--------SASAQQCGRQANSAVCQNGLCCSEHGWCGTTDAYCGAGCQS 67
           AF  +SL LLL        +  +Q CG       C   LCCS+ G+CGT DAYCG GC+S
Sbjct: 2   AFTKISLVLLLCLLGFFSETVKSQNCG-------CAPNLCCSQFGYCGTDDAYCGVGCRS 61

Query: 68  QCRRPGPTPTLPPNAGSGVGRIITAAVFDQLLKYRNDPRCRSNGFYTYNAFLTAARSFPA 127
              R   TPT     G  VG I+T   F+ ++    +  C    FYT ++F+ AA +FP 
Sbjct: 62  GPCRGSGTPT-----GGSVGSIVTQGFFNNIINQAGN-GCAGKRFYTRDSFVNAANTFPN 121

Query: 128 FGTTGDDATRRRELAAFLAQTSHETTGGWPTAPDGPYAWGYCFVRELRQDA--YCRSPDP 187
           F     ++  RRE+A   A  +HET               +C++ E+      YC+S + 
Sbjct: 122 FA----NSVTRREIATMFAHFTHET-------------GHFCYIEEINGATRNYCQSSNT 181

Query: 188 EWPCAPGQKYFGRGPIQLTHNYNYGQAGRALDLNLLNNPDLVATDPIVSFRTAIWFWMTP 247
           ++PCAPG+ YFGRGPIQL+ NYNYG  G++L L+LL  P+LV ++P V+FRT +WFWM  
Sbjct: 182 QYPCAPGKGYFGRGPIQLSWNYNYGACGQSLGLDLLRQPELVGSNPTVAFRTGLWFWMN- 241

Query: 248 QGNKPSSHDVIIGRWQPSNVDRAAGRAAGYGVITNIINGEFECGSGPNDRATSRIGFYTS 307
                S   V+                 G+G     ING  EC  G +    +RIG+Y  
Sbjct: 242 -----SVRPVL---------------NQGFGATIRAING-MECNGGNSGAVNARIGYYRD 264

Query: 308 YCNLLGVTMGPNLDC 313
           YC  LGV  GPNL C
Sbjct: 302 YCGQLGVDPGPNLSC 264

BLAST of CmaCh07G003910 vs. TAIR 10
Match: AT2G43610.1 (Chitinase family protein )

HSP 1 Score: 202.6 bits (514), Expect = 4.8e-52
Identity = 120/312 (38.46%), Postives = 159/312 (50.96%), Query Frame = 0

Query: 9   FIFLSLALLLSASAQQCGRQANSAVCQNGLCCSEHGWCGTTDAYCGAGCQS-QCR-RPGP 68
           F+F    +  +A +Q CG       C+  +CCS  G+CGTT AYCG GCQS  C  +P P
Sbjct: 15  FLFTLTIMTGTAFSQNCGTNG----CKGNMCCSRWGYCGTTKAYCGTGCQSGPCNSKPKP 74

Query: 69  TPTLPPNAGSGVG------RIITAAVFDQLLKYRNDPRCRSNGFYTYNAFLTAARSFPAF 128
           TPT   + G   G       +IT A F+ ++  +    C + GFYT  AF+ AA SF A+
Sbjct: 75  TPTPSGSGGLNAGPRGTIASVITPAFFNSIMS-KVGSGCPAKGFYTRQAFIAAAESFAAY 134

Query: 129 GTTGDDATRRRELAAFLAQTSHETTGGWPTAPDGPYAWGYCFVRELRQDAYCRSPDPEWP 188
             T      +RE+AA LAQ SHE             +  +C+  E+ +  YC SP   +P
Sbjct: 135 KGT----VAKREIAAMLAQFSHE-------------SGSFCYKEEIARGRYC-SPSTTYP 194

Query: 189 CAPGQKYFGRGPIQLTHNYNYGQAGRALDLNLLNNPDLVATDPIVSFRTAIWFWMTPQGN 248
           C PG+ Y+GRGPIQ+T NYNYG AG+ L L LL +PD+VA  P V+F+ A+WFW      
Sbjct: 195 CQPGKNYYGRGPIQITWNYNYGAAGKFLGLPLLKDPDMVARSPTVAFQCAMWFW------ 254

Query: 249 KPSSHDVIIGRWQPSNVDRAAGRAAGYGVITNIINGEFECGSGPNDRATSRIGFYTSYCN 308
                          N +     + G+G  T  ING  EC  G      SR+  Y  +C 
Sbjct: 255 ---------------NKNVRPVLSQGFGATTRRINGG-ECNGGRPAAVQSRVNHYLDFCK 281

Query: 309 LLGVTMGPNLDC 313
            LGVT G NL C
Sbjct: 315 KLGVTPGTNLSC 281

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P369073.6e-12966.77Endochitinase OS=Pisum sativum OX=3888 PE=2 SV=1[more]
Q7DNA17.0e-12564.49Chitinase 2 OS=Oryza sativa subsp. japonica OX=39947 GN=Cht2 PE=1 SV=1[more]
P082521.2e-12466.12Endochitinase A OS=Nicotiana tabacum OX=4097 GN=CHN48 PE=1 SV=2[more]
P240913.9e-12363.66Endochitinase B OS=Nicotiana tabacum OX=4097 GN=CHN50 PE=1 SV=1[more]
Q055383.6e-12163.35Basic 30 kDa endochitinase OS=Solanum lycopersicum OX=4081 GN=CHI9 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1KUS63.3e-194100.00endochitinase-like OS=Cucurbita maxima OX=3661 GN=LOC111497387 PE=4 SV=1[more]
A0A6J1EJE51.1e-18696.84endochitinase-like OS=Cucurbita moschata OX=3662 GN=LOC111433851 PE=4 SV=1[more]
A0A6J1BU425.9e-14374.61endochitinase A-like OS=Momordica charantia OX=3673 GN=LOC111005566 PE=4 SV=1[more]
Q207U13.8e-14274.76Chitinase OS=Momordica charantia OX=3673 GN=ChiA PE=4 SV=1[more]
A0A6J1GD143.7e-13771.84endochitinase-like OS=Cucurbita moschata OX=3662 GN=LOC111453081 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
XP_023003944.16.9e-194100.00endochitinase-like [Cucurbita maxima][more]
KAG6594739.12.8e-18797.15hypothetical protein SDJN03_11292, partial [Cucurbita argyrosperma subsp. sorori... [more]
XP_022926868.12.4e-18696.84endochitinase-like [Cucurbita moschata][more]
XP_023518615.16.4e-18495.31endochitinase-like [Cucurbita pepo subsp. pepo][more]
XP_022132794.11.2e-14274.61endochitinase A-like [Momordica charantia][more]
Match NameE-valueIdentityDescription
AT3G12500.12.2e-11861.88basic chitinase [more]
AT4G01700.13.1e-8355.65Chitinase family protein [more]
AT1G02360.13.7e-8152.87Chitinase family protein [more]
AT2G43590.12.5e-5338.10Chitinase family protein [more]
AT2G43610.14.8e-5238.46Chitinase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001002Chitin-binding, type 1PRINTSPR00451CHITINBINDNGcoord: 40..47
score: 70.0
coord: 47..54
score: 66.33
coord: 32..40
score: 59.56
IPR001002Chitin-binding, type 1SMARTSM00270ChitinBD_3coord: 24..61
e-value: 1.1E-15
score: 68.1
IPR001002Chitin-binding, type 1PFAMPF00187Chitin_bind_1coord: 24..61
e-value: 4.2E-13
score: 49.5
IPR001002Chitin-binding, type 1PROSITEPS50941CHIT_BIND_I_2coord: 22..63
score: 10.627268
IPR036861Endochitinase-like superfamilyGENE3D3.30.60.10coord: 22..63
e-value: 1.6E-16
score: 62.1
IPR036861Endochitinase-like superfamilySUPERFAMILY57016Plant lectins/antimicrobial peptidescoord: 24..71
NoneNo IPR availableGENE3D1.10.530.10coord: 86..318
e-value: 2.0E-114
score: 383.0
NoneNo IPR availableGENE3D3.30.20.10Endochitinase, domain 2coord: 164..221
e-value: 2.0E-114
score: 383.0
NoneNo IPR availablePIRSRPIRSR001060-2PIRSR001060-2coord: 4..320
e-value: 5.6E-139
score: 460.9
NoneNo IPR availablePANTHERPTHR22595:SF171CHITINASE (CLASS IB) / HEVEINcoord: 6..318
NoneNo IPR availablePANTHERPTHR22595CHITINASE-RELATEDcoord: 6..318
NoneNo IPR availableCDDcd00325chitinase_GH19coord: 82..312
e-value: 1.17539E-109
score: 316.294
NoneNo IPR availableCDDcd00035ChtBD1coord: 24..62
e-value: 4.45515E-16
score: 68.9514
IPR016283Glycoside hydrolase, family 19PIRSFPIRSF001060Endochitinasecoord: 1..319
e-value: 9.3E-124
score: 410.6
IPR000726Glycoside hydrolase, family 19, catalyticPFAMPF00182Glyco_hydro_19coord: 81..312
e-value: 2.5E-120
score: 400.5
IPR000726Glycoside hydrolase, family 19, catalyticPROSITEPS00774CHITINASE_19_2coord: 225..235
IPR000726Glycoside hydrolase, family 19, catalyticPROSITEPS00773CHITINASE_19_1coord: 99..121
IPR018371Chitin-binding, type 1, conserved sitePROSITEPS00026CHIT_BIND_I_1coord: 34..53
IPR023346Lysozyme-like domain superfamilySUPERFAMILY53955Lysozyme-likecoord: 78..319

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh07G003910.1CmaCh07G003910.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
biological_process GO:0016998 cell wall macromolecule catabolic process
biological_process GO:0006032 chitin catabolic process
biological_process GO:0006952 defense response
molecular_function GO:0004568 chitinase activity
molecular_function GO:0008061 chitin binding