CmaCh07G001460 (gene) Cucurbita maxima (Rimu) v1.1

Overview


Name	CmaCh07G001460
Type	gene
Organism	Cucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Description	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein
Location	Cma_Chr07: 754091 .. 754948 (-)
RNA-Seq Expression	CmaCh07G001460
Synteny	CmaCh07G001460

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGATTATTTTGAACCATTTGGCTGAAGCTTTGAAGATGGATGGTGAAGAAATGAGGGAGCTATTTAGAGATGGTGTTCAGTCAATGAGGATGAACTACTATCCACCATGCCCTGAACCCGATAAGGCCATCGGATTCTCGGCTCACTCGGATGCAAATGCTCTAACAATTCTCCTGCAACTCAATGAAACAGAAGGACTGCAAATCCGGAAAGATGGGAGATGGGTTTCTGTCAAACCAATTCCAAATGCATTTGTGGTCAATATAGGTAACATCATAGAGGTACGTATAATGAGTTAGTCTTTGGATCGTTGGTGATGAAATTTGTTACTATAATTTGGGCTGTGTTTGTTAATGCACAGATTGTAAGCAATGGCGTATACAAGAGCATTGAGCATAGAGTGACATCAAATTGTTCAAAAGAAAGGCTTTCAGTTGCAACATTCCACGGTCCAAATCTAAGCTTGGAGTTGGGTCCTGCAAAGAGTGTTGTAGGGCCTCATAACCCACCGAAGTTTCGAACTGTCCCTTTGGTGAAGTACTTCAAAGGCTACTTTGCTAAAAAAGTAGAAGGAAAGTCATACCTTGATCAAATTAGAATTCAGAACCAATGAAGTAAGTTGCAGTTAGGACACACTCACTATCGCCACTCGGCCAAATTCATGGAGTCCTGGGTTGTCTTGGGTTTAGAGACCACTTGTTGTGCACGGATGATACAACCTTCAATTCTCTCTTATACCACTGGTTGTAATCTAATAGCACAATTAAAATGATTTAATCTCCCATTGTTGTAGCGGAATGTAACAATCAAAATATTCTAATATTCAATTGTTGTAACTGAAGCAATATCGATCGCAC

mRNA sequence

ATGATTATTTTGAACCATTTGGCTGAAGCTTTGAAGATGGATGGTGAAGAAATGAGGGAGCTATTTAGAGATGGTGTTCAGTCAATGAGGATGAACTACTATCCACCATGCCCTGAACCCGATAAGGCCATCGGATTCTCGGCTCACTCGGATGCAAATGCTCTAACAATTCTCCTGCAACTCAATGAAACAGAAGGACTGCAAATCCGGAAAGATGGGAGATGGGTTTCTGTCAAACCAATTCCAAATGCATTTGTGGTCAATATAGGTAACATCATAGAGATTGTAAGCAATGGCGTATACAAGAGCATTGAGCATAGAGTGACATCAAATTGTTCAAAAGAAAGGCTTTCAGTTGCAACATTCCACGGTCCAAATCTAAGCTTGGAGTTGGGTCCTGCAAAGAGTGTTGTAGGGCCTCATAACCCACCGAAGTTTCGAACTGTCCCTTTGGTGAAGTACTTCAAAGGCTACTTTGCTAAAAAAGTAGAAGGAAAGTCATACCTTGATCAAATTAGAATTCAGAACCAATGAAGTAAGTTGCAGTTAGGACACACTCACTATCGCCACTCGGCCAAATTCATGGAGTCCTGGGTTGTCTTGGGTTTAGAGACCACTTGTTGTGCACGGATGATACAACCTTCAATTCTCTCTTATACCACTGGTTGTAATCTAATAGCACAATTAAAATGATTTAATCTCCCATTGTTGTAGCGGAATGTAACAATCAAAATATTCTAATATTCAATTGTTGTAACTGAAGCAATATCGATCGCAC

Coding sequence (CDS)

ATGATTATTTTGAACCATTTGGCTGAAGCTTTGAAGATGGATGGTGAAGAAATGAGGGAGCTATTTAGAGATGGTGTTCAGTCAATGAGGATGAACTACTATCCACCATGCCCTGAACCCGATAAGGCCATCGGATTCTCGGCTCACTCGGATGCAAATGCTCTAACAATTCTCCTGCAACTCAATGAAACAGAAGGACTGCAAATCCGGAAAGATGGGAGATGGGTTTCTGTCAAACCAATTCCAAATGCATTTGTGGTCAATATAGGTAACATCATAGAGATTGTAAGCAATGGCGTATACAAGAGCATTGAGCATAGAGTGACATCAAATTGTTCAAAAGAAAGGCTTTCAGTTGCAACATTCCACGGTCCAAATCTAAGCTTGGAGTTGGGTCCTGCAAAGAGTGTTGTAGGGCCTCATAACCCACCGAAGTTTCGAACTGTCCCTTTGGTGAAGTACTTCAAAGGCTACTTTGCTAAAAAAGTAGAAGGAAAGTCATACCTTGATCAAATTAGAATTCAGAACCAATGA

Protein sequence

MIILNHLAEALKMDGEEMRELFRDGVQSMRMNYYPPCPEPDKAIGFSAHSDANALTILLQLNETEGLQIRKDGRWVSVKPIPNAFVVNIGNIIEIVSNGVYKSIEHRVTSNCSKERLSVATFHGPNLSLELGPAKSVVGPHNPPKFRTVPLVKYFKGYFAKKVEGKSYLDQIRIQNQ

Homology

[Close]

BLAST of CmaCh07G001460 vs. ExPASy Swiss-Prot
Match: Q39224 (Protein SRG1 OS=Arabidopsis thaliana OX=3702 GN=SRG1 PE=2 SV=1)

HSP 1 Score: 206.5 bits (524), Expect = 2.6e-52
Identity = 93/175 (53.14%), Postives = 133/175 (76.00%), Query Frame = 0


Query: 2   IILNHLAEALKMDGEEMRELFR--DGVQSMRMNYYPPCPEPDKAIGFSAHSDANALTILL 61
           I++  +A AL++  EE+ +LF   D VQSMRMNYYPPCP+PD+ IG + HSD+  LT+L+
Sbjct: 184 ILIAKMARALEIKPEELEKLFDDVDSVQSMRMNYYPPCPQPDQVIGLTPHSDSVGLTVLM 243

Query: 62  QLNETEGLQIRKDGRWVSVKPIPNAFVVNIGNIIEIVSNGVYKSIEHRVTSNCSKERLSV 121
           Q+N+ EGLQI+KDG+WV VKP+PNAF+VNIG+++EI++NG Y+SIEHR   N  KERLS+
Sbjct: 244 QVNDVEGLQIKKDGKWVPVKPLPNAFIVNIGDVLEIITNGTYRSIEHRGVVNSEKERLSI 303

Query: 122 ATFHGPNLSLELGPAKSVVGPHNPPKFRTVPLVKYFKGYFAKKVEGKSYLDQIRI 175
           ATFH   +  E+GPAKS+V      +F+ + + +Y  G F++ ++GK+YLD +RI
Sbjct: 304 ATFHNVGMYKEVGPAKSLVERQKVARFKRLTMKEYNDGLFSRTLDGKAYLDALRI 358

[Close]

BLAST of CmaCh07G001460 vs. ExPASy Swiss-Prot
Match: D4N501 (Probable 2-oxoglutarate/Fe(II)-dependent dioxygenase OS=Papaver somniferum OX=3469 GN=DIOX2 PE=2 SV=1)

HSP 1 Score: 201.8 bits (512), Expect = 6.3e-51
Identity = 90/178 (50.56%), Postives = 134/178 (75.28%), Query Frame = 0


Query: 1   MIILNHLAEALKMDGEEMREL---FRDGVQSMRMNYYPPCPEPDKAIGFSAHSDANALTI 60
           M++   + +AL++   E++E+   F+D  Q MRMNYYPPCP+P+ AIG + HSD   LTI
Sbjct: 187 MVLFEKMEKALQVQAVEIKEISEVFKDMTQVMRMNYYPPCPQPELAIGLTPHSDFGGLTI 246

Query: 61  LLQLNETEGLQIRKDGRWVSVKPIPNAFVVNIGNIIEIVSNGVYKSIEHRVTSNCSKERL 120
           LLQLNE EGLQI+ +GRW+SVKP+PNAFVVN+G+++EI++NG+Y+S++HR   N +KERL
Sbjct: 247 LLQLNEVEGLQIKNEGRWISVKPLPNAFVVNVGDVLEIMTNGMYRSVDHRAVVNSTKERL 306

Query: 121 SVATFHGPNLSLELGPAKSVVGPHNPPKFRTVPLV-KYFKGYFAKKVEGKSYLDQIRI 175
           S+ATFH PNL  E+GP  S++ P+ P  FR+     +  + + ++K++GKS+LD +R+
Sbjct: 307 SIATFHDPNLESEIGPISSLITPNTPALFRSGSTYGELVEEFHSRKLDGKSFLDSMRM 364

[Close]

BLAST of CmaCh07G001460 vs. ExPASy Swiss-Prot
Match: D4N502 (Codeine O-demethylase OS=Papaver somniferum OX=3469 GN=CODM PE=1 SV=1)

HSP 1 Score: 196.4 bits (498), Expect = 2.7e-49
Identity = 87/174 (50.00%), Postives = 129/174 (74.14%), Query Frame = 0


Query: 2   IILNHLAEALKM-DGEEMRELFRDGVQSMRMNYYPPCPEPDKAIGFSAHSDANALTILLQ 61
           ++   L ++L++ + + M +LF DG+Q+MRMNYYPPCP P+  +G ++HSD + LTILLQ
Sbjct: 187 VVFEMLEKSLQLVEIKGMTDLFEDGLQTMRMNYYPPCPRPELVLGLTSHSDFSGLTILLQ 246

Query: 62  LNETEGLQIRKDGRWVSVKPIPNAFVVNIGNIIEIVSNGVYKSIEHRVTSNCSKERLSVA 121
           LNE EGLQIRK+ RW+S+KP+P+AF+VN+G+I+EI++NG+Y+S+EHR   N +KERLS+A
Sbjct: 247 LNEVEGLQIRKEERWISIKPLPDAFIVNVGDILEIMTNGIYRSVEHRAVVNSTKERLSIA 306

Query: 122 TFHGPNLSLELGPAKSVVGPHNPPKFRTVPLVKYFKGYFAKKVEGKSYLDQIRI 175
           TFH   L  E+GP  S+V P  P  F+        K   ++K++GKS+LD +R+
Sbjct: 307 TFHDSKLESEIGPISSLVTPETPALFKRGRYEDILKENLSRKLDGKSFLDYMRM 360

[Close]

BLAST of CmaCh07G001460 vs. ExPASy Swiss-Prot
Match: D4N500 (Thebaine 6-O-demethylase OS=Papaver somniferum OX=3469 GN=T6ODM PE=1 SV=1)

HSP 1 Score: 196.1 bits (497), Expect = 3.5e-49
Identity = 92/181 (50.83%), Postives = 132/181 (72.93%), Query Frame = 0


Query: 1   MIILNHLAEALKMDGEE---MRELFRDGVQSMRMNYYPPCPEPDKAIGFSAHSDANALTI 60
           M++ N + +AL++   E   M E+F DG Q+MRMNYYPPCP+P+ AIG ++HSD   LTI
Sbjct: 187 MVLFNKMEKALQVQAAEIKGMSEVFIDGTQAMRMNYYPPCPQPNLAIGLTSHSDFGGLTI 246

Query: 61  LLQLNETEGLQIRKDGRWVSVKPIPNAFVVNIGNIIEIVSNGVYKSIEHRVTSNCSKERL 120
           LLQ+NE EGLQI+++G W+SVKP+PNAFVVN+G+I+EI++NG+Y S++HR   N + ERL
Sbjct: 247 LLQINEVEGLQIKREGTWISVKPLPNAFVVNVGDILEIMTNGIYHSVDHRAVVNSTNERL 306

Query: 121 SVATFHGPNLSLELGPAKSVVGPHNPPKFRT----VPLVKYFKGYFAKKVEGKSYLDQIR 175
           S+ATFH P+L   +GP  S++ P  P  F++      LV+  K    +K++GKS+LD +R
Sbjct: 307 SIATFHDPSLESVIGPISSLITPETPALFKSGSTYGDLVEECK---TRKLDGKSFLDSMR 364

[Close]

BLAST of CmaCh07G001460 vs. ExPASy Swiss-Prot
Match: Q8W2X5 (Flavanone 3-dioxygenase 2 OS=Oryza sativa subsp. japonica OX=39947 GN=F3H-2 PE=1 SV=1)

HSP 1 Score: 151.4 bits (381), Expect = 9.8e-36
Identity = 69/167 (41.32%), Postives = 109/167 (65.27%), Query Frame = 0


Query: 7   LAEALKMDGEEMRELFRDGVQSMRMNYYPPCPEPDKAIGFSAHSDANALTILLQLNETEG 66
           ++E+L ++G  MRE   +  Q M +NYYP CPEP+   G  AH+D NALTILL  ++  G
Sbjct: 175 ISESLGLEGGYMRETLGEQEQHMAVNYYPQCPEPELTYGLPAHTDPNALTILLMDDQVAG 234

Query: 67  LQIRKDGRWVSVKPIPNAFVVNIGNIIEIVSNGVYKSIEHRVTSNCSKERLSVATFHGPN 126
           LQ+  DG+W++V P P A V+NIG+ ++ +SNG Y+S+ HR   N  +ER+SVA+F  P 
Sbjct: 235 LQVLNDGKWIAVNPQPGALVINIGDQLQALSNGKYRSVWHRAVVNSDRERMSVASFLCPC 294

Query: 127 LSLELGPAKSVVGPHNPPKFRTVPLVKYFKGYFAKKVEGKSYLDQIR 174
            S+ELGPAK ++   +P  +R     +Y+K ++++ ++ +  L+  R
Sbjct: 295 NSVELGPAKKLITDDSPAVYRNYTYDEYYKKFWSRNLDQEHCLELFR 341

[Close]

BLAST of CmaCh07G001460 vs. ExPASy TrEMBL
Match: A0A6J1EEZ5 (protein SRG1-like OS=Cucurbita moschata OX=3662 GN=LOC111433604 PE=3 SV=1)

HSP 1 Score: 355.1 bits (910), Expect = 1.6e-94
Identity = 173/175 (98.86%), Postives = 174/175 (99.43%), Query Frame = 0


Query: 1   MIILNHLAEALKMDGEEMRELFRDGVQSMRMNYYPPCPEPDKAIGFSAHSDANALTILLQ 60
           MIILNHLAEALKMDGEEMRELFRDGVQSMRMNYYPPCPEPDKAIGFSAHSDANALTILLQ
Sbjct: 183 MIILNHLAEALKMDGEEMRELFRDGVQSMRMNYYPPCPEPDKAIGFSAHSDANALTILLQ 242

Query: 61  LNETEGLQIRKDGRWVSVKPIPNAFVVNIGNIIEIVSNGVYKSIEHRVTSNCSKERLSVA 120
           LNETEGLQIRKDGRWVSVKPIPNAFVVNIGNIIEIVSNGVYKSIEHRVTSNCSKERLSVA
Sbjct: 243 LNETEGLQIRKDGRWVSVKPIPNAFVVNIGNIIEIVSNGVYKSIEHRVTSNCSKERLSVA 302

Query: 121 TFHGPNLSLELGPAKSVVGPHNPPKFRTVPLVKYFKGYFAKKVEGKSYLDQIRIQ 176
           TFH PNLSLELGPAKSVVGPHNPPKFRTVPLVKYFKGYFAKKVEGKSYLDQIR+Q
Sbjct: 303 TFHSPNLSLELGPAKSVVGPHNPPKFRTVPLVKYFKGYFAKKVEGKSYLDQIRMQ 357

[Close]

BLAST of CmaCh07G001460 vs. ExPASy TrEMBL
Match: A0A6J1EG75 (protein SRG1-like OS=Cucurbita moschata OX=3662 GN=LOC111433084 PE=3 SV=1)

HSP 1 Score: 295.4 bits (755), Expect = 1.5e-76
Identity = 142/176 (80.68%), Postives = 160/176 (90.91%), Query Frame = 0


Query: 1   MIILNHLAEALKMDGEEMRELFRDGVQSMRMNYYPPCPEPDKAIGFSAHSDANALTILLQ 60
           ++IL+HLA+ALKMD EEMRELFRDGVQSMR+NYYPPCPEPDKAIGFSAHSDA+ALTIL Q
Sbjct: 184 IVILDHLAKALKMDVEEMRELFRDGVQSMRLNYYPPCPEPDKAIGFSAHSDADALTILYQ 243

Query: 61  LNETEGLQIRKDGRWVSVKPIPNAFVVNIGNIIEIVSNGVYKSIEHRVTSNCSKERLSVA 120
           LNE EGLQIRKDGRWVSVKP+PNAFV NIG+I+EIVSNGVYKSIEHRV+ N SKERLSVA
Sbjct: 244 LNEAEGLQIRKDGRWVSVKPLPNAFVANIGDIMEIVSNGVYKSIEHRVSLNFSKERLSVA 303

Query: 121 TFHGPNLSLELGPAKSVVGPHNPPKFRTVPLVKYFKGYFAKKVEGKSYLDQIRIQN 177
           TFH  NL+ ELGPA S+VGPHNP  FR V L KYFK +FA+K+EGKSYL+Q+RI+N
Sbjct: 304 TFHSSNLNSELGPANSLVGPHNPAVFRRVMLEKYFKDFFARKLEGKSYLEQMRIEN 359

[Close]

BLAST of CmaCh07G001460 vs. ExPASy TrEMBL
Match: A0A6J1ILW1 (protein SRG1-like OS=Cucurbita maxima OX=3661 GN=LOC111478593 PE=3 SV=1)

HSP 1 Score: 291.6 bits (745), Expect = 2.2e-75
Identity = 140/176 (79.55%), Postives = 160/176 (90.91%), Query Frame = 0


Query: 1   MIILNHLAEALKMDGEEMRELFRDGVQSMRMNYYPPCPEPDKAIGFSAHSDANALTILLQ 60
           ++IL+HLA+ALKMD EEMRELFRDGVQSMR+NYYPPCPEPDKAIGFSAHSDA+ALTIL Q
Sbjct: 183 IVILDHLAKALKMDVEEMRELFRDGVQSMRLNYYPPCPEPDKAIGFSAHSDADALTILYQ 242

Query: 61  LNETEGLQIRKDGRWVSVKPIPNAFVVNIGNIIEIVSNGVYKSIEHRVTSNCSKERLSVA 120
           LNE EGLQIRKDGRWV+VKP+PNAFVVNIG+I+EIVSNGVYKSIEHRV+ N SKERLSVA
Sbjct: 243 LNEAEGLQIRKDGRWVAVKPLPNAFVVNIGDIMEIVSNGVYKSIEHRVSLNFSKERLSVA 302

Query: 121 TFHGPNLSLELGPAKSVVGPHNPPKFRTVPLVKYFKGYFAKKVEGKSYLDQIRIQN 177
           TFH  NL+ +LGPA S+VGP NP  FR V L KYFK +FA+K+EGKSYL+Q+RI+N
Sbjct: 303 TFHSSNLNSDLGPANSLVGPQNPAVFRRVMLEKYFKDFFARKLEGKSYLEQMRIEN 358

[Close]

BLAST of CmaCh07G001460 vs. ExPASy TrEMBL
Match: A0A0A0KLU1 (Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G520990 PE=3 SV=1)

HSP 1 Score: 286.6 bits (732), Expect = 7.2e-74
Identity = 138/177 (77.97%), Postives = 158/177 (89.27%), Query Frame = 0


Query: 1   MIILNHLAEALKMDGEEMRELFRDGVQSMRMNYYPPCPEPDKAIGFSAHSDANALTILLQ 60
           M+IL HLAEALKMD EEMRELF DGVQS+RMNYYPPCP PDKAIGFSAHSDA+ALTIL Q
Sbjct: 183 MVILGHLAEALKMDVEEMRELFGDGVQSVRMNYYPPCPVPDKAIGFSAHSDADALTILYQ 242

Query: 61  LNETEGLQIRKDGRWVSVKPIPNAFVVNIGNIIEIVSNGVYKSIEHRVTSNCSKERLSVA 120
           LNE EGLQIRKDGRWVSVKP+PNAFVVNIG+I+EIVSNGVYKSIEHRV+SN SKERLSVA
Sbjct: 243 LNEVEGLQIRKDGRWVSVKPLPNAFVVNIGDIMEIVSNGVYKSIEHRVSSNFSKERLSVA 302

Query: 121 TFHGPNLSLELGPAKSVVGPHNPPKFRTVPLVKYFKGYFAKKVEGKSYLDQIRIQNQ 178
           TF+  N++ ELGPAKS++GPHNP  FR V L KYF+ +FA+K+E KSYL+ +RI+ +
Sbjct: 303 TFYSSNINSELGPAKSLIGPHNPAVFRRVLLEKYFRDFFARKLERKSYLEHMRIETE 359

[Close]

BLAST of CmaCh07G001460 vs. ExPASy TrEMBL
Match: A0A5D3CMW3 (Protein SRG1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G008600 PE=3 SV=1)

HSP 1 Score: 283.9 bits (725), Expect = 4.7e-73
Identity = 135/177 (76.27%), Postives = 160/177 (90.40%), Query Frame = 0


Query: 1   MIILNHLAEALKMDGEEMRELFRDGVQSMRMNYYPPCPEPDKAIGFSAHSDANALTILLQ 60
           M+IL+HLA ALKMD EEMRELF DGVQS+RMNYYPPCP PDKAIGFSAHSDA+ALTIL Q
Sbjct: 183 MVILDHLAGALKMDVEEMRELFGDGVQSVRMNYYPPCPVPDKAIGFSAHSDADALTILYQ 242

Query: 61  LNETEGLQIRKDGRWVSVKPIPNAFVVNIGNIIEIVSNGVYKSIEHRVTSNCSKERLSVA 120
           LNE EGLQIRKDGRW++VKP+PNAFVVNIG+I+EIVSNGVYKSIEHRV+SN SKERLSVA
Sbjct: 243 LNEVEGLQIRKDGRWIAVKPLPNAFVVNIGDIMEIVSNGVYKSIEHRVSSNFSKERLSVA 302

Query: 121 TFHGPNLSLELGPAKSVVGPHNPPKFRTVPLVKYFKGYFAKKVEGKSYLDQIRIQNQ 178
           TF+  NL+ E+GPAKS++GPHNP  F+ V L KYF+ +FA+K+EGKSYL+ +RI+++
Sbjct: 303 TFYSLNLNSEVGPAKSLIGPHNPAVFQRVMLEKYFRDFFARKLEGKSYLEHMRIESE 359

[Close]

BLAST of CmaCh07G001460 vs. NCBI nr
Match: KAG6594509.1 (Protein SRG1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 359.0 bits (920), Expect = 2.4e-95
Identity = 174/177 (98.31%), Postives = 176/177 (99.44%), Query Frame = 0


Query: 1   MIILNHLAEALKMDGEEMRELFRDGVQSMRMNYYPPCPEPDKAIGFSAHSDANALTILLQ 60
           MIILNHLAEALKMDGEEMRELFRDG+QSMRMNYYPPCPEPDKAIGFSAHSDANALTILLQ
Sbjct: 183 MIILNHLAEALKMDGEEMRELFRDGIQSMRMNYYPPCPEPDKAIGFSAHSDANALTILLQ 242

Query: 61  LNETEGLQIRKDGRWVSVKPIPNAFVVNIGNIIEIVSNGVYKSIEHRVTSNCSKERLSVA 120
           LNETEGLQIRKDGRWVSVKPIPNAFVVNIGNIIEIVSNGVYKSIEHRVTSNCSKERLSVA
Sbjct: 243 LNETEGLQIRKDGRWVSVKPIPNAFVVNIGNIIEIVSNGVYKSIEHRVTSNCSKERLSVA 302

Query: 121 TFHGPNLSLELGPAKSVVGPHNPPKFRTVPLVKYFKGYFAKKVEGKSYLDQIRIQNQ 178
           TFH PNLSLELGPAKSVVGPHNPPKFRTVPLVKYFKGYFAKKVEGKSYLDQIR+QNQ
Sbjct: 303 TFHSPNLSLELGPAKSVVGPHNPPKFRTVPLVKYFKGYFAKKVEGKSYLDQIRMQNQ 359

[Close]

BLAST of CmaCh07G001460 vs. NCBI nr
Match: KAG7026495.1 (Protein SRG1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 358.2 bits (918), Expect = 4.0e-95
Identity = 174/177 (98.31%), Postives = 176/177 (99.44%), Query Frame = 0


Query: 1   MIILNHLAEALKMDGEEMRELFRDGVQSMRMNYYPPCPEPDKAIGFSAHSDANALTILLQ 60
           MIILNHLAEALKMDGEEMRELFRDGVQSMRMNYYPPCPEPDKAIGFSAHSDANALTILLQ
Sbjct: 410 MIILNHLAEALKMDGEEMRELFRDGVQSMRMNYYPPCPEPDKAIGFSAHSDANALTILLQ 469

Query: 61  LNETEGLQIRKDGRWVSVKPIPNAFVVNIGNIIEIVSNGVYKSIEHRVTSNCSKERLSVA 120
           LNETEGLQIRKDGRWVSVKPIPNAFVVNIGNIIEIVSNGVYKSIEHRVTSNCSK+RLSVA
Sbjct: 470 LNETEGLQIRKDGRWVSVKPIPNAFVVNIGNIIEIVSNGVYKSIEHRVTSNCSKQRLSVA 529

Query: 121 TFHGPNLSLELGPAKSVVGPHNPPKFRTVPLVKYFKGYFAKKVEGKSYLDQIRIQNQ 178
           TFH PNLSLELGPAKSVVGPHNPPKFRTVPLVKYFKGYFAKKVEGKSYLDQIR+QNQ
Sbjct: 530 TFHSPNLSLELGPAKSVVGPHNPPKFRTVPLVKYFKGYFAKKVEGKSYLDQIRMQNQ 586

[Close]

BLAST of CmaCh07G001460 vs. NCBI nr
Match: XP_022926461.1 (protein SRG1-like [Cucurbita moschata])

HSP 1 Score: 355.1 bits (910), Expect = 3.4e-94
Identity = 173/175 (98.86%), Postives = 174/175 (99.43%), Query Frame = 0


Query: 1   MIILNHLAEALKMDGEEMRELFRDGVQSMRMNYYPPCPEPDKAIGFSAHSDANALTILLQ 60
           MIILNHLAEALKMDGEEMRELFRDGVQSMRMNYYPPCPEPDKAIGFSAHSDANALTILLQ
Sbjct: 183 MIILNHLAEALKMDGEEMRELFRDGVQSMRMNYYPPCPEPDKAIGFSAHSDANALTILLQ 242

Query: 61  LNETEGLQIRKDGRWVSVKPIPNAFVVNIGNIIEIVSNGVYKSIEHRVTSNCSKERLSVA 120
           LNETEGLQIRKDGRWVSVKPIPNAFVVNIGNIIEIVSNGVYKSIEHRVTSNCSKERLSVA
Sbjct: 243 LNETEGLQIRKDGRWVSVKPIPNAFVVNIGNIIEIVSNGVYKSIEHRVTSNCSKERLSVA 302

Query: 121 TFHGPNLSLELGPAKSVVGPHNPPKFRTVPLVKYFKGYFAKKVEGKSYLDQIRIQ 176
           TFH PNLSLELGPAKSVVGPHNPPKFRTVPLVKYFKGYFAKKVEGKSYLDQIR+Q
Sbjct: 303 TFHSPNLSLELGPAKSVVGPHNPPKFRTVPLVKYFKGYFAKKVEGKSYLDQIRMQ 357

[Close]

BLAST of CmaCh07G001460 vs. NCBI nr
Match: XP_023518165.1 (uncharacterized protein LOC111781709 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 353.6 bits (906), Expect = 9.9e-94
Identity = 172/177 (97.18%), Postives = 174/177 (98.31%), Query Frame = 0


Query: 1   MIILNHLAEALKMDGEEMRELFRDGVQSMRMNYYPPCPEPDKAIGFSAHSDANALTILLQ 60
           MIILNHLAEALKMDGEEMRELFRDGVQSMRMNYYPPCPEPDKAIGFSAHSDANALTILLQ
Sbjct: 556 MIILNHLAEALKMDGEEMRELFRDGVQSMRMNYYPPCPEPDKAIGFSAHSDANALTILLQ 615

Query: 61  LNETEGLQIRKDGRWVSVKPIPNAFVVNIGNIIEIVSNGVYKSIEHRVTSNCSKERLSVA 120
           LNETEGLQIRKDGRWVSVKPIPNAFVVNIGNIIEIVSNGVYKSIEHRVTSNC KERLSVA
Sbjct: 616 LNETEGLQIRKDGRWVSVKPIPNAFVVNIGNIIEIVSNGVYKSIEHRVTSNCLKERLSVA 675

Query: 121 TFHGPNLSLELGPAKSVVGPHNPPKFRTVPLVKYFKGYFAKKVEGKSYLDQIRIQNQ 178
           TFH PNLSLELGPAKSVVGPHNPPKFR VPLV+YFKGYFAKKVEGKSYLDQIR+QNQ
Sbjct: 676 TFHSPNLSLELGPAKSVVGPHNPPKFRAVPLVEYFKGYFAKKVEGKSYLDQIRMQNQ 732

[Close]

BLAST of CmaCh07G001460 vs. NCBI nr
Match: XP_023544157.1 (protein SRG1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 295.8 bits (756), Expect = 2.5e-76
Identity = 142/176 (80.68%), Postives = 161/176 (91.48%), Query Frame = 0


Query: 1   MIILNHLAEALKMDGEEMRELFRDGVQSMRMNYYPPCPEPDKAIGFSAHSDANALTILLQ 60
           ++IL+HLA+ALKMD EEMRELFRDGVQSMR+NYYPPCPEPDKAIGFSAHSDA+ALTIL Q
Sbjct: 182 IVILDHLAKALKMDVEEMRELFRDGVQSMRLNYYPPCPEPDKAIGFSAHSDADALTILYQ 241

Query: 61  LNETEGLQIRKDGRWVSVKPIPNAFVVNIGNIIEIVSNGVYKSIEHRVTSNCSKERLSVA 120
           LNE EGLQIRKDGRWV+VKP+PNAFVVNIG+I+EIVSNGVYKSIEHRV+ N SKERLSVA
Sbjct: 242 LNEAEGLQIRKDGRWVAVKPLPNAFVVNIGDIMEIVSNGVYKSIEHRVSLNFSKERLSVA 301

Query: 121 TFHGPNLSLELGPAKSVVGPHNPPKFRTVPLVKYFKGYFAKKVEGKSYLDQIRIQN 177
           TFH  NL+ ELGPA S+VGPHNP  FR V L KYFK +FA+K+EGKSYL+Q+RI+N
Sbjct: 302 TFHSSNLNSELGPANSLVGPHNPAVFRRVMLEKYFKDFFARKLEGKSYLEQMRIEN 357

[Close]

BLAST of CmaCh07G001460 vs. TAIR 10
Match: AT4G25310.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 215.3 bits (547), Expect = 3.9e-56
Identity = 101/174 (58.05%), Postives = 132/174 (75.86%), Query Frame = 0


Query: 2   IILNHLAEALKMDGEEMRELFRDGV-QSMRMNYYPPCPEPDKAIGFSAHSDANALTILLQ 61
           ++   LA ALK+  EEM +LF D + Q +RMNYYPPCPEPDKAIG + HSDA  LTILLQ
Sbjct: 180 VLFAKLASALKIKPEEMEKLFDDELGQRIRMNYYPPCPEPDKAIGLTPHSDATGLTILLQ 239

Query: 62  LNETEGLQIRKDGRWVSVKPIPNAFVVNIGNIIEIVSNGVYKSIEHRVTSNCSKERLSVA 121
           +NE EGLQI+KDG+WVSVKP+PNA VVN+G+I+EI++NG Y+SIEHR   N  KERLSVA
Sbjct: 240 VNEVEGLQIKKDGKWVSVKPLPNALVVNVGDILEIITNGTYRSIEHRGVVNSEKERLSVA 299

Query: 122 TFHGPNLSLELGPAKSVVGPHNPPKFRTVPLVKYFKGYFAKKVEGKSYLDQIRI 175
           +FH      E+GP +S+V  H    F+T+   +YF G F+++++GK+YLD +RI
Sbjct: 300 SFHNTGFGKEIGPMRSLVERHKGALFKTLTTEEYFHGLFSRELDGKAYLDVMRI 353

[Close]

BLAST of CmaCh07G001460 vs. TAIR 10
Match: AT1G17020.1 (senescence-related gene 1 )

HSP 1 Score: 206.5 bits (524), Expect = 1.8e-53
Identity = 93/175 (53.14%), Postives = 133/175 (76.00%), Query Frame = 0


Query: 2   IILNHLAEALKMDGEEMRELFR--DGVQSMRMNYYPPCPEPDKAIGFSAHSDANALTILL 61
           I++  +A AL++  EE+ +LF   D VQSMRMNYYPPCP+PD+ IG + HSD+  LT+L+
Sbjct: 184 ILIAKMARALEIKPEELEKLFDDVDSVQSMRMNYYPPCPQPDQVIGLTPHSDSVGLTVLM 243

Query: 62  QLNETEGLQIRKDGRWVSVKPIPNAFVVNIGNIIEIVSNGVYKSIEHRVTSNCSKERLSV 121
           Q+N+ EGLQI+KDG+WV VKP+PNAF+VNIG+++EI++NG Y+SIEHR   N  KERLS+
Sbjct: 244 QVNDVEGLQIKKDGKWVPVKPLPNAFIVNIGDVLEIITNGTYRSIEHRGVVNSEKERLSI 303

Query: 122 ATFHGPNLSLELGPAKSVVGPHNPPKFRTVPLVKYFKGYFAKKVEGKSYLDQIRI 175
           ATFH   +  E+GPAKS+V      +F+ + + +Y  G F++ ++GK+YLD +RI
Sbjct: 304 ATFHNVGMYKEVGPAKSLVERQKVARFKRLTMKEYNDGLFSRTLDGKAYLDALRI 358

[Close]

BLAST of CmaCh07G001460 vs. TAIR 10
Match: AT4G25300.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 203.0 bits (515), Expect = 2.0e-52
Identity = 96/174 (55.17%), Postives = 129/174 (74.14%), Query Frame = 0


Query: 2   IILNHLAEALKMDGEEMRELFRDGV-QSMRMNYYPPCPEPDKAIGFSAHSDANALTILLQ 61
           I+L  +A ALK+  EEM +LF D + Q +R+NYYP CPEPDK IG + HSD+  LTILLQ
Sbjct: 183 ILLGKIAVALKIKPEEMDKLFDDELGQRIRLNYYPRCPEPDKVIGLTPHSDSTGLTILLQ 242

Query: 62  LNETEGLQIRKDGRWVSVKPIPNAFVVNIGNIIEIVSNGVYKSIEHRVTSNCSKERLSVA 121
            NE EGLQI+K+ +WVSVKP+PNA VVN+G+I+EI++NG Y+SIEHR   N  KERLSVA
Sbjct: 243 ANEVEGLQIKKNAKWVSVKPLPNALVVNVGDILEIITNGTYRSIEHRGVVNSEKERLSVA 302

Query: 122 TFHGPNLSLELGPAKSVVGPHNPPKFRTVPLVKYFKGYFAKKVEGKSYLDQIRI 175
            FH   L  E+GP +S+V  H    F++V   +YF G F+++++GK+YLD +R+
Sbjct: 303 AFHNIGLGKEIGPMRSLVERHKAAFFKSVTTEEYFNGLFSRELDGKAYLDVMRL 356

[Close]

BLAST of CmaCh07G001460 vs. TAIR 10
Match: AT4G25300.2 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 203.0 bits (515), Expect = 2.0e-52
Identity = 96/174 (55.17%), Postives = 129/174 (74.14%), Query Frame = 0


Query: 2   IILNHLAEALKMDGEEMRELFRDGV-QSMRMNYYPPCPEPDKAIGFSAHSDANALTILLQ 61
           I+L  +A ALK+  EEM +LF D + Q +R+NYYP CPEPDK IG + HSD+  LTILLQ
Sbjct: 89  ILLGKIAVALKIKPEEMDKLFDDELGQRIRLNYYPRCPEPDKVIGLTPHSDSTGLTILLQ 148

Query: 62  LNETEGLQIRKDGRWVSVKPIPNAFVVNIGNIIEIVSNGVYKSIEHRVTSNCSKERLSVA 121
            NE EGLQI+K+ +WVSVKP+PNA VVN+G+I+EI++NG Y+SIEHR   N  KERLSVA
Sbjct: 149 ANEVEGLQIKKNAKWVSVKPLPNALVVNVGDILEIITNGTYRSIEHRGVVNSEKERLSVA 208

Query: 122 TFHGPNLSLELGPAKSVVGPHNPPKFRTVPLVKYFKGYFAKKVEGKSYLDQIRI 175
            FH   L  E+GP +S+V  H    F++V   +YF G F+++++GK+YLD +R+
Sbjct: 209 AFHNIGLGKEIGPMRSLVERHKAAFFKSVTTEEYFNGLFSRELDGKAYLDVMRL 262

[Close]

BLAST of CmaCh07G001460 vs. TAIR 10
Match: AT1G17010.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 200.3 bits (508), Expect = 1.3e-51
Identity = 91/177 (51.41%), Postives = 131/177 (74.01%), Query Frame = 0


Query: 2   IILNHLAEALKMDGEEMRELFRDG-VQSMRMNYYPPCPEPDKAIGFSAHSDANALTILLQ 61
           I+L  +A+AL++  EE+ E+F D  +QSMRMNYYPPCP+P+   G   HSDA  LTILLQ
Sbjct: 185 ILLAKMAKALQIKPEEVEEIFGDDMMQSMRMNYYPPCPQPNLVTGLIPHSDAVGLTILLQ 244

Query: 62  LNETEGLQIRKDGRWVSVKPIPNAFVVNIGNIIEIVSNGVYKSIEHRVTSNCSKERLSVA 121
           +NE +GLQI+K+G+W  VKP+ NAF+VN+G+++EI++NG Y+SIEHR   N  KERLS+A
Sbjct: 245 VNEVDGLQIKKNGKWFFVKPLQNAFIVNVGDVLEIITNGTYRSIEHRAMVNLEKERLSIA 304

Query: 122 TFHGPNLSLELGPAKSVVGPHNPPKFRTVPLVKYFKGYFAKKVEGKSYLDQIRIQNQ 178
           TFH   +  E+GPA+S+V      KFR++    Y  G F+++++GK+YLD +RI+ +
Sbjct: 305 TFHNTGMDKEIGPARSLVQRQEAAKFRSLKTKDYLNGLFSRELKGKAYLDAMRIEGK 361

The following BLAST results are available for this feature:

BLAST of CmaCh07G001460 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q39224	2.6e-52	53.14	Protein SRG1 OS=Arabidopsis thaliana OX=3702 GN=SRG1 PE=2 SV=1	[more]
D4N501	6.3e-51	50.56	Probable 2-oxoglutarate/Fe(II)-dependent dioxygenase OS=Papaver somniferum OX=34...	[more]
D4N502	2.7e-49	50.00	Codeine O-demethylase OS=Papaver somniferum OX=3469 GN=CODM PE=1 SV=1	[more]
D4N500	3.5e-49	50.83	Thebaine 6-O-demethylase OS=Papaver somniferum OX=3469 GN=T6ODM PE=1 SV=1	[more]
Q8W2X5	9.8e-36	41.32	Flavanone 3-dioxygenase 2 OS=Oryza sativa subsp. japonica OX=39947 GN=F3H-2 PE=1...	[more]

BLAST of CmaCh07G001460 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1EEZ5	1.6e-94	98.86	protein SRG1-like OS=Cucurbita moschata OX=3662 GN=LOC111433604 PE=3 SV=1	[more]
A0A6J1EG75	1.5e-76	80.68	protein SRG1-like OS=Cucurbita moschata OX=3662 GN=LOC111433084 PE=3 SV=1	[more]
A0A6J1ILW1	2.2e-75	79.55	protein SRG1-like OS=Cucurbita maxima OX=3661 GN=LOC111478593 PE=3 SV=1	[more]
A0A0A0KLU1	7.2e-74	77.97	Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G...	[more]
A0A5D3CMW3	4.7e-73	76.27	Protein SRG1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G00...	[more]

BLAST of CmaCh07G001460 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG6594509.1	2.4e-95	98.31	Protein SRG1, partial [Cucurbita argyrosperma subsp. sororia]	[more]
KAG7026495.1	4.0e-95	98.31	Protein SRG1 [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_022926461.1	3.4e-94	98.86	protein SRG1-like [Cucurbita moschata]	[more]
XP_023518165.1	9.9e-94	97.18	uncharacterized protein LOC111781709 [Cucurbita pepo subsp. pepo]	[more]
XP_023544157.1	2.5e-76	80.68	protein SRG1-like [Cucurbita pepo subsp. pepo]	[more]

BLAST of CmaCh07G001460 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G25310.1	3.9e-56	58.05	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT1G17020.1	1.8e-53	53.14	senescence-related gene 1	[more]
AT4G25300.1	2.0e-52	55.17	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT4G25300.2	2.0e-52	55.17	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT1G17010.1	1.3e-51	51.41	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR027443	Isopenicillin N synthase-like superfamily	GENE3D	2.60.120.330		coord: 1..166 e-value: 2.5E-55 score: 190.0
IPR044861	Isopenicillin N synthase-like, Fe(2+) 2OG dioxygenase domain	PFAM	PF03171	2OG-FeII_Oxy	coord: 27..125 e-value: 1.8E-29 score: 102.2
None	No IPR available	PANTHER	PTHR47991:SF4	PROTEIN SRG1-LIKE	coord: 1..174
None	No IPR available	PANTHER	PTHR47991	OXOGLUTARATE/IRON-DEPENDENT DIOXYGENASE	coord: 1..174
None	No IPR available	SUPERFAMILY	51197	Clavaminate synthase-like	coord: 2..165
IPR005123	Oxoglutarate/iron-dependent dioxygenase	PROSITE	PS51471	FE2OG_OXY	coord: 25..125 score: 15.117553

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmaCh07G001460.1	CmaCh07G001460.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0051213	dioxygenase activity
molecular_function	GO:0046872	metal ion binding