CmaCh06G016120 (gene) Cucurbita maxima (Rimu) v1.1

Overview


Name	CmaCh06G016120
Type	gene
Organism	Cucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Description	splicing factor 3B subunit 4-like
Location	Cma_Chr06: 10032264 .. 10037012 (+)
RNA-Seq Expression	CmaCh06G016120
Synteny	CmaCh06G016120

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AGGAACTCCTTCGACTCTCCCCAGCCTACAAGAGGTTTTAGATTGTAGTTTCTGATCCCCAAACGCTGCGACTGGTTCACCGACTCGCCGGAGAAGATGACGACGAAGATAGCGCCGGGAGTCGGAGCTAATCTGCTAGGCCAGCACGCCCCCGAGAGGAACCAAGACGCCACTGCCTATGTCGGCAATTTAGATCCCCAGGTCCTTTGTTCTCTTCCTCCCTGCTTTTTCAACTAATCCATCTTCAAATCCCCTCCTCTAGGGTTTCCATGTTCATTTAATTTAATTTTTTTTTTTTTTTTTTTTTTTTTTTGTGTGTGTTTCTTGTAGGTTTCTGAGGAGTTGCTTTGGGAGCTGTTTGTTCAAGCCGGCCCAGTTGGTGCGTTCTTTCGTTTCTCTCGTTTTGTGAAGTCTTTGGAATTTATGGAAACTCTGTTTGAGCTGTCTTTTTCCCTTCTTGGTTTGAGCACTTCCTTGAAAAAAATTGTGCCCACTTGTTATTTTGCAGTTAATGTGTACGTTCCGAAGGATAGAGTTACTAACTTGCATCAAGGATATGGATTCATTGAGTTTCGCAGTGAGGAAGATGCAGATTATGTAAGTAACGCTACGACTCTTTTTCTTTTCTATCCTCCTAATTATGTGTGTATTTTGATTTCCCATTCTTAATGCTTTTGCAGGCAATTAAGGTGCTGAATATGATTAAGCTCTACGGGAAGCCAATTCGAGTAAACAAGGTGTGTAGGGAATTTATTCTTTCAAGTGTGCTAGGATGCGTTTTGATATACTGCTCAGCAAAGGAAGATGGTATTTTGTATTCTTATGAAAATGGATATCTTACTGTTGTTATGTGTGCATTGCATATTTCCAGGCGTCTCAAGACAAGAAGAGTTTGGATGTGGGAGCAAATCTCTTCATAGGAAACCTCGACCCCGTGAGTAGTTATTTTATCATGTTTATATTTGGAAGACTTGCAAGCTAATGATTTAAAGAAGGTAACTAAACCTTGACGTTTGTTTCAGGATGTTGATGAAAAGCTCCTCTACGATACTTTCAGTGCTTTTGGGGTCATTGTGACAAATCCTAAGGTTATAACTTTTCCTATAAACCTCATCACTCATTTATGTTTTGTTTATCTTAGGAGATTAGGGCTCATTTTCAAGCAAACTAGTTACTTTCTAAGGAAGTAATCTTATTACAACTAAATGAAGTGAAGTTCTTTCATGGAAATACTAGACCTTTACATTGCCTTTTAGTATATTTCACATGTATATGATATCCTTGGATGTTTATCGCTTCTGGAGCGTGTTGAACATAGTTCGTCTCTAGCCTTTATTTTCTTTATGGGGCTGCTAAACCTTGGAAGTTTGTGGTTATTTTTGGCTTTTATTTATGGTTAAATCATAAATAGGAAAATTTGGGCCCTATGGTTAGGGAAAAAAAATTATAATTTAAACTCTATGGTCTTAAAAGTAAGAATTTAGTCGTTATGGTATGATAAGTCATAAATAGTTTCTATGGTTCGATAATACTCTCATAAATAAGGACTAAGATAACACTTAAATCCATGGGGACTTGATTCTAGCTTTCTTTAGAGACCAAATTTGTAATTTAACCTTTAATAATTTATTTCTTTGTGATTTTGTGATGATTATGAAGTGAATCCTGAAAATTCATTCCTACCAAATATTTTGGCTGTTTGGCCAAAAGATTTCTCTGGACTATAAAACTGGACTCTTGAATTTCTGATGTCCAATAAAAGGTCTAGGGAGTTCAAGATAAGGGGAAGGGAAGACAAGATCTTAGAAATCTTTTAGCCAAGTAGATAATGCATTTATACATGTCTAAAAGTTAAATTTGTAGAATTTATTTCATGGCCACTCCGCATAAGATTCTGTAGTTGCTTTTGTGAACTGTAGCATTGTATTTAGTAAATCCATTGTCTACCATTTCTTGCCCCCCCCCCAAAAAAAAAAAAAAAAAAAAAGAATGAAGAATTACAGTTTTAGTAGAATACCGTTATGGTGTTATTATTATATGGATCACTGTAATTTTCCAATTTCTCGTTCTTTTGTTTTATCTTTCTTTCTCAGTATAATGCTTCTTTGGTTGAAGTAAATTGACATGTCTCTCCTATTTTAAGCATAAGGGTGATTGGTAGCAGCCAGTTTATGGTTTGGTTTCTTTTCTCTTTAACAAGTGTCTTTCCTAATAAAACATCATTTTAAATGTTTTCTCTTCCATTATTATTTTATTTTCGTGTATTACTTGGATTTACTTTATATTTTCTAGGATTTTGTAACTGGTTTTTCTTCCATGTGGGCTAGTACATCTTACTAGTACTTACCAGTATTATCATGTTTGTGAAAATGGGACTAGTTTTATGTCCTGGCTTTCTATATAATATTATTATCTTGAAGTTCTTGTAGTTGGGTACGCCAGATTGGAAATTTGTTTGGGCTTTTAGGGGGTTAATAACATCATGGCTGTTGGGAGGCGTCTATATTTTGCTTACTGAACGTCTATCAGATCAGTTTCCTTCCATGCTTCAAGAGCAACGCAGAACTAGAGTTTTGTGTGCATAAGACACCTCGCTATTCCTTTATTTTTTAACTAAAGATCATTTCTTTTTGTATGCTCTATTTTTGATCTTTAGTTCTCTGTATCTTCTGTGTACTCGGCTCTTTTTGGGGATGTATCAGAACGGTTTAACATGAAAGTTCTGAGTTTTATGCACATTTGTGTTCTAACACTTCAAATGTCTTCGTGTTTCTAACCAGATTATGAGAGATCCAGATACGGGAAATTCACGTGGGTTTGGTTTTATTAGCTACGACTCTTTTGAAGCATCTGATGCAGCAATAGAGGTGTGTCTTTTCTTTTCTTATTGGTCGATCTCTACACGCATGTCATTCTATTTTGATACTATATGATGCTCATAATAAGCTTCCCTTAAATCATTGGAGCCTTCAGTGCCCCTAAATGTTAATGAAGTGAAAGAAGTGTTTTATTGCTACCTATCCTTTGTAATGCTCTCGAAAAATAAGGATCTATTTTTTCACGTCATCCAATTCATAAGGAAAACTCTGTCTCCAAATTTTGTTCAAGAAAGTTGTCCAAGTCTTTTAGCCCACTCAGTGAAGTACGTTTCCTTTTCAATTTCTGTCCCTTCACTTTTCTTCCCCCCGTTCATTGCATCCAAGTTGTTTTCTCCTCCTTGAATCAGATTCTTCATTAACCAGAGGAATGATCCATTCGACAAAAGACCCTACTTCTCCTTCTCATGAAAATGGTTTTGATGCTGAATCTGATGTTAGCTTGAGTAGTATGGGGTTGAATTTGCAACCTTTGAATAGCTACTTAAAAGATGATGTTTTTGAAGATTCTTATTAGAAAGATTTTGCTTCTTTATTTCAAGGTGATTAAAGATAATCCTCCTCCTCTCTTATTGAACTTTGTGGTTTCAAGATGTGCTAAAGTTCTCTATTGGTTAAACATGGAAACAGTGAAAAACATTGCCCCTCTAAGCTCTTAGGTCCTGTTTTCGTGGTTTTCTTTCCACTTTCCATGAATTTTGTTTTGTTTGCTATTCTTGTGGAAATTTCAGCTTCTTTATCAGTTGATTTCCTTCGTGGCTTCGGTTCAAGCTTTTATCTCCAGATGGTTTTGACTTTTGTTTTCTTGTTCTGTGGTTGCTGTCGTTAACAGGCAATGAATGGCCAGTATCTTTGTAATCGTCAAATAACGGTTTCTTATGCCTATAAGAAAGATACCAAGGGTGAACGACATGGCACACCTGCGGGTAAGAATTGTTTGCTGATCCATGAACTTTATGGAATTGTTTTAGGTTATGAACAGATTTGAAACTAGTGATCTGTGGAAGGATATTGTTGTCGTTGTTGTTGCTTATTTATCTCTGGTGTGGCTTTGTCTATTTTGTAGAGAGAGTATTGGCTGCTAGCAATCCCCAGAAGAGTAGACCTCACACTTTGTTTGCAAGTGGCCCTCCGACACTTCCAAAGGTTGCACAGCCTAACGGCACGATGGGAACTCTGATGCCACCACGCCCCTTCGCTAATGGTGGCATTACTCCCAGCCCAATTCCTGCTATACGTCCTCCACCACCGCAAGGGGTGGCTTTCCCCCCTATGCAGATGCCTGGACAACCACCAGCCTGGCAGGGTCAGCCGCAGCAACAAGGTCAAATTATGCCGGGTTCCATGATGCTACCCCCTGTTCAGCAATTTCGGCCACCTCCCCCAAACATGCCTCCGCCACCACCTCAGGCTCCTGGTCATTCCATGCCTATCCCTCCACCAATGGGAATGGGAGGAGGGCAGCCACCTCTTTGGCGACCACCACCACCGCCAATGCAGCAGCAGCAGCAGCAGCAGCCGGGAAGGCCCCATATGCAAATGTCGTCGATGCCCCCACCGCCACCTCCAAATCACATTCCACCACCTCATCCATCTTCTGGCTAAGACATTATTATACTACGCTAGCAATGAGAATGAAGTTTTTTCTGCCTGAACATCATGTTTAGTTATTTTTTATGATAATGCCCAGTAGGTTGTTATATTATCATTCAAATTGTAACACTTTCATCCGAGAAATTAGCAGGAAGCAATATGACATTTTTGCTACAAAATAGAGAATGTTCTCATCTTAGTTTGGGATACTTGTCAAGCTTCTAATGCACATTAGTAGAGTATCAATGAATATAGGCAAAGACCATGTAGGTGACCATCA

mRNA sequence

AGGAACTCCTTCGACTCTCCCCAGCCTACAAGAGGTTTTAGATTGTAGTTTCTGATCCCCAAACGCTGCGACTGGTTCACCGACTCGCCGGAGAAGATGACGACGAAGATAGCGCCGGGAGTCGGAGCTAATCTGCTAGGCCAGCACGCCCCCGAGAGGAACCAAGACGCCACTGCCTATGTCGGCAATTTAGATCCCCAGGTTTCTGAGGAGTTGCTTTGGGAGCTGTTTGTTCAAGCCGGCCCAGTTGTTAATGTGTACGTTCCGAAGGATAGAGTTACTAACTTGCATCAAGGATATGGATTCATTGAGTTTCGCAGTGAGGAAGATGCAGATTATGCAATTAAGGTGCTGAATATGATTAAGCTCTACGGGAAGCCAATTCGAGTAAACAAGGCGTCTCAAGACAAGAAGAGTTTGGATGTGGGAGCAAATCTCTTCATAGGAAACCTCGACCCCGATGTTGATGAAAAGCTCCTCTACGATACTTTCAGTGCTTTTGGGGTCATTGTGACAAATCCTAAGATTATGAGAGATCCAGATACGGGAAATTCACGTGGGTTTGGTTTTATTAGCTACGACTCTTTTGAAGCATCTGATGCAGCAATAGAGGCAATGAATGGCCAGTATCTTTGTAATCGTCAAATAACGGTTTCTTATGCCTATAAGAAAGATACCAAGGGTGAACGACATGGCACACCTGCGGAGAGAGTATTGGCTGCTAGCAATCCCCAGAAGAGTAGACCTCACACTTTGTTTGCAAGTGGCCCTCCGACACTTCCAAAGGTTGCACAGCCTAACGGCACGATGGGAACTCTGATGCCACCACGCCCCTTCGCTAATGGTGGCATTACTCCCAGCCCAATTCCTGCTATACGTCCTCCACCACCGCAAGGGGTGGCTTTCCCCCCTATGCAGATGCCTGGACAACCACCAGCCTGGCAGGGTCAGCCGCAGCAACAAGGTCAAATTATGCCGGGTTCCATGATGCTACCCCCTGTTCAGCAATTTCGGCCACCTCCCCCAAACATGCCTCCGCCACCACCTCAGGCTCCTGGTCATTCCATGCCTATCCCTCCACCAATGGGAATGGGAGGAGGGCAGCCACCTCTTTGGCGACCACCACCACCGCCAATGCAGCAGCAGCAGCAGCAGCAGCCGGGAAGGCCCCATATGCAAATGTCGTCGATGCCCCCACCGCCACCTCCAAATCACATTCCACCACCTCATCCATCTTCTGGCTAAGACATTATTATACTACGCTAGCAATGAGAATGAAGTTTTTTCTGCCTGAACATCATGTTTAGTTATTTTTTATGATAATGCCCAGTAGGTTGTTATATTATCATTCAAATTGTAACACTTTCATCCGAGAAATTAGCAGGAAGCAATATGACATTTTTGCTACAAAATAGAGAATGTTCTCATCTTAGTTTGGGATACTTGTCAAGCTTCTAATGCACATTAGTAGAGTATCAATGAATATAGGCAAAGACCATGTAGGTGACCATCA

Coding sequence (CDS)

ATGACGACGAAGATAGCGCCGGGAGTCGGAGCTAATCTGCTAGGCCAGCACGCCCCCGAGAGGAACCAAGACGCCACTGCCTATGTCGGCAATTTAGATCCCCAGGTTTCTGAGGAGTTGCTTTGGGAGCTGTTTGTTCAAGCCGGCCCAGTTGTTAATGTGTACGTTCCGAAGGATAGAGTTACTAACTTGCATCAAGGATATGGATTCATTGAGTTTCGCAGTGAGGAAGATGCAGATTATGCAATTAAGGTGCTGAATATGATTAAGCTCTACGGGAAGCCAATTCGAGTAAACAAGGCGTCTCAAGACAAGAAGAGTTTGGATGTGGGAGCAAATCTCTTCATAGGAAACCTCGACCCCGATGTTGATGAAAAGCTCCTCTACGATACTTTCAGTGCTTTTGGGGTCATTGTGACAAATCCTAAGATTATGAGAGATCCAGATACGGGAAATTCACGTGGGTTTGGTTTTATTAGCTACGACTCTTTTGAAGCATCTGATGCAGCAATAGAGGCAATGAATGGCCAGTATCTTTGTAATCGTCAAATAACGGTTTCTTATGCCTATAAGAAAGATACCAAGGGTGAACGACATGGCACACCTGCGGAGAGAGTATTGGCTGCTAGCAATCCCCAGAAGAGTAGACCTCACACTTTGTTTGCAAGTGGCCCTCCGACACTTCCAAAGGTTGCACAGCCTAACGGCACGATGGGAACTCTGATGCCACCACGCCCCTTCGCTAATGGTGGCATTACTCCCAGCCCAATTCCTGCTATACGTCCTCCACCACCGCAAGGGGTGGCTTTCCCCCCTATGCAGATGCCTGGACAACCACCAGCCTGGCAGGGTCAGCCGCAGCAACAAGGTCAAATTATGCCGGGTTCCATGATGCTACCCCCTGTTCAGCAATTTCGGCCACCTCCCCCAAACATGCCTCCGCCACCACCTCAGGCTCCTGGTCATTCCATGCCTATCCCTCCACCAATGGGAATGGGAGGAGGGCAGCCACCTCTTTGGCGACCACCACCACCGCCAATGCAGCAGCAGCAGCAGCAGCAGCCGGGAAGGCCCCATATGCAAATGTCGTCGATGCCCCCACCGCCACCTCCAAATCACATTCCACCACCTCATCCATCTTCTGGCTAA

Protein sequence

MTTKIAPGVGANLLGQHAPERNQDATAYVGNLDPQVSEELLWELFVQAGPVVNVYVPKDRVTNLHQGYGFIEFRSEEDADYAIKVLNMIKLYGKPIRVNKASQDKKSLDVGANLFIGNLDPDVDEKLLYDTFSAFGVIVTNPKIMRDPDTGNSRGFGFISYDSFEASDAAIEAMNGQYLCNRQITVSYAYKKDTKGERHGTPAERVLAASNPQKSRPHTLFASGPPTLPKVAQPNGTMGTLMPPRPFANGGITPSPIPAIRPPPPQGVAFPPMQMPGQPPAWQGQPQQQGQIMPGSMMLPPVQQFRPPPPNMPPPPPQAPGHSMPIPPPMGMGGGQPPLWRPPPPPMQQQQQQQPGRPHMQMSSMPPPPPPNHIPPPHPSSG

Homology

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BLAST of CmaCh06G016120 vs. ExPASy Swiss-Prot
Match: Q15427 (Splicing factor 3B subunit 4 OS=Homo sapiens OX=9606 GN=SF3B4 PE=1 SV=1)

HSP 1 Score: 339.0 bits (868), Expect = 7.1e-92
Identity = 218/402 (54.23%), Postives = 253/402 (62.94%), Query Frame = 0


Query: 20  ERNQDATAYVGNLDPQVSEELLWELFVQAGPVVNVYVPKDRVTNLHQGYGFIEFRSEEDA 79
           ERNQDAT YVG LD +VSE LLWELF+QAGPVVN ++PKDRVT  HQGYGF+EF SEEDA
Sbjct: 8   ERNQDATVYVGGLDEKVSEPLLWELFLQAGPVVNTHMPKDRVTGQHQGYGFVEFLSEEDA 67

Query: 80  DYAIKVLNMIKLYGKPIRVNKASQDKKSLDVGANLFIGNLDPDVDEKLLYDTFSAFGVIV 139
           DYAIK++NMIKLYGKPIRVNKAS   K+LDVGAN+FIGNLDP++DEKLLYDTFSAFGVI+
Sbjct: 68  DYAIKIMNMIKLYGKPIRVNKASAHNKNLDVGANIFIGNLDPEIDEKLLYDTFSAFGVIL 127

Query: 140 TNPKIMRDPDTGNSRGFGFISYDSFEASDAAIEAMNGQYLCNRQITVSYAYKKDTKGERH 199
             PKIMRDPDTGNS+G+ FI++ SF+ASDAAIEAMNGQYLCNR ITVSYA+KKD+KGERH
Sbjct: 128 QTPKIMRDPDTGNSKGYAFINFASFDASDAAIEAMNGQYLCNRPITVSYAFKKDSKGERH 187

Query: 200 GTPAERVLAASNP--QKSRPHTLFASGPPTLPKVAQP-NGTMGTLMPPRPFANGGITPSP 259
           G+ AER+LAA NP  Q  RPH LFA  PP  P    P   ++G+ +PP      G  P P
Sbjct: 188 GSAAERLLAAQNPLSQADRPHQLFADAPPP-PSAPNPVVSSLGSGLPPPGMPPPGSFPPP 247

Query: 260 IPAIRPPPPQGV--AFPPMQMP----GQPPAWQGQP---------QQQGQIMPGSMMLPP 319
           +P     PP G+  A PP  MP    G  P   G P                PG M  P 
Sbjct: 248 VPPPGALPP-GIPPAMPPPPMPPGAAGHGPPSAGTPGAGHPGHGHSHPHPFPPGGMPHPG 307

Query: 320 VQQFR---------------PPPPNMPPPPPQAPGHSMPIPPPMGMGGGQP----PLWRP 379
           + Q +               PP     PPP   PG   P PPPMGM    P    P+  P
Sbjct: 308 MSQMQLAHHGPHGLGHPHAGPPGSGGQPPPRPPPGMPHPGPPPMGMPPRGPPFGSPMGHP 367

Query: 380 PPPPMQQQQQQQPGRPHMQMSSMPPPPPPNH----IPPPHPS 381
            P P    +   P  P    +  P PPP  +    +PPP P+
Sbjct: 368 GPMPPHGMRGPPPLMPPHGYTGPPRPPPYGYQRGPLPPPRPT 407

[Close]

BLAST of CmaCh06G016120 vs. ExPASy Swiss-Prot
Match: Q8QZY9 (Splicing factor 3B subunit 4 OS=Mus musculus OX=10090 GN=Sf3b4 PE=1 SV=1)

HSP 1 Score: 339.0 bits (868), Expect = 7.1e-92
Identity = 218/402 (54.23%), Postives = 253/402 (62.94%), Query Frame = 0


Query: 20  ERNQDATAYVGNLDPQVSEELLWELFVQAGPVVNVYVPKDRVTNLHQGYGFIEFRSEEDA 79
           ERNQDAT YVG LD +VSE LLWELF+QAGPVVN ++PKDRVT  HQGYGF+EF SEEDA
Sbjct: 8   ERNQDATVYVGGLDEKVSEPLLWELFLQAGPVVNTHMPKDRVTGQHQGYGFVEFLSEEDA 67

Query: 80  DYAIKVLNMIKLYGKPIRVNKASQDKKSLDVGANLFIGNLDPDVDEKLLYDTFSAFGVIV 139
           DYAIK++NMIKLYGKPIRVNKAS   K+LDVGAN+FIGNLDP++DEKLLYDTFSAFGVI+
Sbjct: 68  DYAIKIMNMIKLYGKPIRVNKASAHNKNLDVGANIFIGNLDPEIDEKLLYDTFSAFGVIL 127

Query: 140 TNPKIMRDPDTGNSRGFGFISYDSFEASDAAIEAMNGQYLCNRQITVSYAYKKDTKGERH 199
             PKIMRDPDTGNS+G+ FI++ SF+ASDAAIEAMNGQYLCNR ITVSYA+KKD+KGERH
Sbjct: 128 QTPKIMRDPDTGNSKGYAFINFASFDASDAAIEAMNGQYLCNRPITVSYAFKKDSKGERH 187

Query: 200 GTPAERVLAASNP--QKSRPHTLFASGPPTLPKVAQP-NGTMGTLMPPRPFANGGITPSP 259
           G+ AER+LAA NP  Q  RPH LFA  PP  P    P   ++G+ +PP      G  P P
Sbjct: 188 GSAAERLLAAQNPLSQADRPHQLFADAPPP-PSAPNPVVSSLGSGLPPPGMPPPGSFPPP 247

Query: 260 IPAIRPPPPQGV--AFPPMQMP----GQPPAWQGQP---------QQQGQIMPGSMMLPP 319
           +P     PP G+  A PP  MP    G  P   G P                PG M  P 
Sbjct: 248 VPPPGALPP-GIPPAMPPPPMPPGAGGHGPPAAGTPGAGHPGHGHSHPHPFPPGGMPHPG 307

Query: 320 VQQFR---------------PPPPNMPPPPPQAPGHSMPIPPPMGMGGGQP----PLWRP 379
           + Q +               PP     PPP   PG   P PPPMGM    P    P+  P
Sbjct: 308 MSQMQLAHHGPHGLGHPHAGPPGSGGQPPPRPPPGMPHPGPPPMGMPPRGPPFGSPMGHP 367

Query: 380 PPPPMQQQQQQQPGRPHMQMSSMPPPPPPNH----IPPPHPS 381
            P P    +   P  P    +  P PPP  +    +PPP P+
Sbjct: 368 GPMPPHGMRGPPPLMPPHGYTGPPRPPPYGYQRGPLPPPRPT 407

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BLAST of CmaCh06G016120 vs. ExPASy Swiss-Prot
Match: Q6AYL5 (Splicing factor 3B subunit 4 OS=Rattus norvegicus OX=10116 GN=Sf3b4 PE=2 SV=1)

HSP 1 Score: 339.0 bits (868), Expect = 7.1e-92
Identity = 218/402 (54.23%), Postives = 253/402 (62.94%), Query Frame = 0


Query: 20  ERNQDATAYVGNLDPQVSEELLWELFVQAGPVVNVYVPKDRVTNLHQGYGFIEFRSEEDA 79
           ERNQDAT YVG LD +VSE LLWELF+QAGPVVN ++PKDRVT  HQGYGF+EF SEEDA
Sbjct: 8   ERNQDATVYVGGLDEKVSEPLLWELFLQAGPVVNTHMPKDRVTGQHQGYGFVEFLSEEDA 67

Query: 80  DYAIKVLNMIKLYGKPIRVNKASQDKKSLDVGANLFIGNLDPDVDEKLLYDTFSAFGVIV 139
           DYAIK++NMIKLYGKPIRVNKAS   K+LDVGAN+FIGNLDP++DEKLLYDTFSAFGVI+
Sbjct: 68  DYAIKIMNMIKLYGKPIRVNKASAHNKNLDVGANIFIGNLDPEIDEKLLYDTFSAFGVIL 127

Query: 140 TNPKIMRDPDTGNSRGFGFISYDSFEASDAAIEAMNGQYLCNRQITVSYAYKKDTKGERH 199
             PKIMRDPDTGNS+G+ FI++ SF+ASDAAIEAMNGQYLCNR ITVSYA+KKD+KGERH
Sbjct: 128 QTPKIMRDPDTGNSKGYAFINFASFDASDAAIEAMNGQYLCNRPITVSYAFKKDSKGERH 187

Query: 200 GTPAERVLAASNP--QKSRPHTLFASGPPTLPKVAQP-NGTMGTLMPPRPFANGGITPSP 259
           G+ AER+LAA NP  Q  RPH LFA  PP  P    P   ++G+ +PP      G  P P
Sbjct: 188 GSAAERLLAAQNPLSQADRPHQLFADAPPP-PSAPNPVVSSLGSGLPPPGMPPPGSFPPP 247

Query: 260 IPAIRPPPPQGV--AFPPMQMP----GQPPAWQGQP---------QQQGQIMPGSMMLPP 319
           +P     PP G+  A PP  MP    G  P   G P                PG M  P 
Sbjct: 248 VPPPGALPP-GIPPAMPPPPMPPGAGGHGPPAAGTPGAGHPGHGHSHPHPFPPGGMPHPG 307

Query: 320 VQQFR---------------PPPPNMPPPPPQAPGHSMPIPPPMGMGGGQP----PLWRP 379
           + Q +               PP     PPP   PG   P PPPMGM    P    P+  P
Sbjct: 308 MSQMQLAHHGPHGLGHPHAGPPGSGGQPPPRPPPGMPHPGPPPMGMPPRGPPFGSPMGHP 367

Query: 380 PPPPMQQQQQQQPGRPHMQMSSMPPPPPPNH----IPPPHPS 381
            P P    +   P  P    +  P PPP  +    +PPP P+
Sbjct: 368 GPMPPHGMRGPPPLMPPHGYTGPPRPPPYGYQRGPLPPPRPT 407

[Close]

BLAST of CmaCh06G016120 vs. ExPASy Swiss-Prot
Match: Q09442 (Splicing factor 3B subunit 4 OS=Caenorhabditis elegans OX=6239 GN=sap-49 PE=1 SV=2)

HSP 1 Score: 325.9 bits (834), Expect = 6.3e-88
Identity = 212/385 (55.06%), Postives = 244/385 (63.38%), Query Frame = 0


Query: 20  ERNQDATAYVGNLDPQVSEELLWELFVQAGPVVNVYVPKDRVTNLHQGYGFIEFRSEEDA 79
           ERNQDAT YVG LD +VSE +LWEL VQAGPVV+V +PKDRVT  HQG+GF+EF  EEDA
Sbjct: 8   ERNQDATIYVGGLDEKVSESILWELMVQAGPVVSVNMPKDRVTANHQGFGFVEFMGEEDA 67

Query: 80  DYAIKVLNMIKLYGKPIRVNKASQDKKSLDVGANLFIGNLDPDVDEKLLYDTFSAFGVIV 139
           DYAIK+LNMIKLYGKPI+VNKAS  +K++DVGAN+F+GNLDP+VDEKLLYDTFSAFGVI+
Sbjct: 68  DYAIKILNMIKLYGKPIKVNKASAHEKNMDVGANIFVGNLDPEVDEKLLYDTFSAFGVIL 127

Query: 140 TNPKIMRDPDTGNSRGFGFISYDSFEASDAAIEAMNGQYLCNRQITVSYAYKKDTKGERH 199
             PKIMRD D+G S+GF FI++ SFEASD A+EAMNGQ+LCNR ITVSYA+K+D+KGERH
Sbjct: 128 QVPKIMRDVDSGTSKGFAFINFASFEASDTALEAMNGQFLCNRAITVSYAFKRDSKGERH 187

Query: 200 GTPAERVLAASNP--QKSRPHTLFASGPPTLPK-------------VAQPNGTMGTLMPP 259
           GT AER+LAA NP   K RPH +F+  P  +P               A   G  G   PP
Sbjct: 188 GTAAERMLAAQNPLFPKDRPHQVFSDVPLGVPANTPLAMPGVHAAIAAHATGRPGYQPPP 247

Query: 260 RPFANGGITPSPIPAIRPPPPQGVAFPPMQMPGQPPAWQGQPQQQGQIMPGSMMLPPVQQ 319
                        P + PPPP     PP  MP  P      P       P S M PP   
Sbjct: 248 LMGMAQSGYQGQYPPVPPPPPSVTPMPP-PMPPTPGMTPRPPPP-----PSSGMWPPPP- 307

Query: 320 FRPPPPNMPPPPPQAPGHSMPIPP----PMGMGGGQPPLWRPPPPPMQQQQQQQPGRPHM 379
             PPPP   P PP  PG   P PP    P GM GG PP   PPPP M+      P  P  
Sbjct: 308 --PPPPGRTPGPPGMPGMPPPPPPSRFGPPGM-GGMPP---PPPPGMRYPGGMPPPPPPR 367

Query: 380 QMSSMP---PPPPPNHIPPPHPSSG 383
             S+ P   PPPPP+   PP P SG
Sbjct: 368 YPSAGPGMYPPPPPSR--PPAPPSG 377

[Close]

BLAST of CmaCh06G016120 vs. ExPASy Swiss-Prot
Match: O14102 (Spliceosome-associated protein 49 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=sap49 PE=3 SV=2)

HSP 1 Score: 243.0 bits (619), Expect = 5.3e-63
Identity = 151/326 (46.32%), Postives = 199/326 (61.04%), Query Frame = 0


Query: 20  ERNQDATAYVGNLDPQVSEELLWELFVQAGPVVNVYVPKDRVTNLHQGYGFIEFRSEEDA 79
           +RNQDAT Y+GNLD +V++ +L+EL +QAGPVVN+++P+DRV N H G+GF EF  E+D 
Sbjct: 6   DRNQDATIYLGNLDEKVTDSILFELCLQAGPVVNIHIPRDRVRNSHNGFGFCEFLHEQDV 65

Query: 80  DYAIKVLNMIKLYGKPIRVNKASQDK-KSLDVGANLFIGNLDPDVDEKLLYDTFSAFGVI 139
           +YA ++LN +KL+GKPIRVN+ASQD+  +  +GANLF+GNLDP VDE++LYDTFSA G +
Sbjct: 66  EYACQILNQVKLFGKPIRVNRASQDRGVNTLIGANLFVGNLDPLVDERVLYDTFSALGQL 125

Query: 140 VTNPKIMRDPDTGNSRGFGFISYDSFEASDAAIEAMNGQYLCNRQITVSYAYKKDTKGER 199
           V  P++ RD + G S+G+GF+SYDSFE +DAAIEAMN Q+L N+ ITVSYA+K++ KGER
Sbjct: 126 VKAPQVARD-ENGRSKGYGFVSYDSFETADAAIEAMNNQFLMNKPITVSYAFKREGKGER 185

Query: 200 HGTPAERVLAAS--------NPQKSRPHTLFASGPPTLPKVAQPNGTMGTLMPPRP---- 259
           HG  AER LAA+         PQ + P     + P        P     T +PP P    
Sbjct: 186 HGDIAERKLAAAAKKNKVAVTPQSTLPPGFSPATPAPTSAANTPATIAATSIPPVPNVPL 245

Query: 260 -FANGGITPSPIPAIRPPPPQGVAFPPMQ-MPGQPPAWQGQPQQQGQIMPGSMMLPPVQQ 319
             A   + P  IP + P       F   Q  PG P      P     + PG   L P   
Sbjct: 246 VGATTAVPPLSIPNVLP-------FTAAQHFPGMP----AMPMMNVPMGPGGAPLVP--- 305

Query: 320 FRPPPPNMPPPPPQAPGHSMPIPPPM 331
             PPPP M    P     ++P  P M
Sbjct: 306 --PPPPGMVMASPSPAAATIPGAPVM 314

[Close]

BLAST of CmaCh06G016120 vs. TAIR 10
Match: AT2G18510.1 (RNA-binding (RRM/RBD/RNP motifs) family protein )

HSP 1 Score: 483.8 bits (1244), Expect = 1.3e-136
Identity = 280/382 (73.30%), Postives = 293/382 (76.70%), Query Frame = 0


Query: 1   MTTKIAPGVGANLLGQHAPERNQDATAYVGNLDPQVSEELLWELFVQAGPVVNVYVPKDR 60
           MTT+IAPGVGANLLGQH+ ERNQDAT YVG LD Q+SEELLWELFVQAGPVVNVYVPKDR
Sbjct: 1   MTTRIAPGVGANLLGQHSAERNQDATVYVGGLDAQLSEELLWELFVQAGPVVNVYVPKDR 60

Query: 61  VTNLHQGYGFIEFRSEEDADYAIKVLNMIKLYGKPIRVNKASQDKKSLDVGANLFIGNLD 120
           VTNLHQ YGFIE+RSEEDADYAIKVLNMIKL+GKPIRVNKASQDKKSLDVGANLFIGNLD
Sbjct: 61  VTNLHQNYGFIEYRSEEDADYAIKVLNMIKLHGKPIRVNKASQDKKSLDVGANLFIGNLD 120

Query: 121 PDVDEKLLYDTFSAFGVIVTNPKIMRDPDTGNSRGFGFISYDSFEASDAAIEAMNGQYLC 180
           PDVDEKLLYDTFSAFGVI +NPKIMRDPDTGNSRGFGFISYDSFEASDAAIE+M GQYL 
Sbjct: 121 PDVDEKLLYDTFSAFGVIASNPKIMRDPDTGNSRGFGFISYDSFEASDAAIESMTGQYLS 180

Query: 181 NRQITVSYAYKKDTKGERHGTPAERVLAASNP--QKSRPHTLFASGPPTLPKVAQPNGTM 240
           NRQITVSYAYKKDTKGERHGTPAER+LAA+NP  QKSRPHTLFA GPP+     Q NG  
Sbjct: 181 NRQITVSYAYKKDTKGERHGTPAERLLAATNPTAQKSRPHTLFAMGPPS--SAPQVNGL- 240

Query: 241 GTLMPPRPFANGGITPSPIPAIRPPPPQGVAFPPMQMPGQPPAWQGQPQQQGQIMPGSMM 300
                PRPFANG + P PIPA R PPP     PP     QPP+W  QPQQ         M
Sbjct: 241 -----PRPFANGSMQPVPIPAPRQPPPP----PPQVYQTQPPSWPSQPQQHS-------M 300

Query: 301 LPPVQQFRPPPPNMPPPPPQAPGHSM------PIPPPMGMGGGQPPLWRPPPPPMQQQ-- 360
           +PP  QFRPP    PPPPPQ   H        P PPP  MG  Q   W  PP  MQQQ  
Sbjct: 301 VPPPMQFRPPQGMPPPPPPQFLNHQQGFGGPRPPPPPQAMGMHQHGGW--PPQHMQQQGG 360

Query: 361 --QQQQPGRPHMQMSSMPPPPP 371
             QQQQP   H  M SMPPPPP
Sbjct: 361 PPQQQQPPYQHHHM-SMPPPPP 360

[Close]

BLAST of CmaCh06G016120 vs. TAIR 10
Match: AT4G34110.1 (poly(A) binding protein 2 )

HSP 1 Score: 131.7 bits (330), Expect = 1.2e-30
Identity = 73/164 (44.51%), Postives = 105/164 (64.02%), Query Frame = 0


Query: 24  DATAYVGNLDPQVSEELLWELFVQAGPVVNVYVPKDRVTNLHQGYGFIEFRSEEDADYAI 83
           + + YVG+LD  V++  L++ F Q G VV V V +D VT    GYG++ F + +DA  AI
Sbjct: 35  NTSLYVGDLDFNVTDSQLFDAFGQMGTVVTVRVCRDLVTRRSLGYGYVNFTNPQDAARAI 94

Query: 84  KVLNMIKLYGKPIRVNKASQDKKSLDVGA-NLFIGNLDPDVDEKLLYDTFSAFGVIVTNP 143
           + LN I LYGKPIRV  + +D      GA N+FI NLD  +D K L+DTFS+FG IV + 
Sbjct: 95  QELNYIPLYGKPIRVMYSHRDPSVRRSGAGNIFIKNLDESIDHKALHDTFSSFGNIV-SC 154

Query: 144 KIMRDPDTGNSRGFGFISYDSFEASDAAIEAMNGQYLCNRQITV 187
           K+  D  +G S+G+GF+ Y + E++  AIE +NG  L ++Q+ V
Sbjct: 155 KVAVD-SSGQSKGYGFVQYANEESAQKAIEKLNGMLLNDKQVYV 196

[Close]

BLAST of CmaCh06G016120 vs. TAIR 10
Match: AT1G49760.1 (poly(A) binding protein 8 )

HSP 1 Score: 131.3 bits (329), Expect = 1.6e-30
Identity = 71/175 (40.57%), Postives = 109/175 (62.29%), Query Frame = 0


Query: 28  YVGNLDPQVSEELLWELFVQAGPVVNVYVPKDRVTNLHQGYGFIEFRSEEDADYAIKVLN 87
           YVG+LD  V++  L+E F QAG VV+V V +D  T    GYG++ + + +DA  A+  LN
Sbjct: 48  YVGDLDATVTDSQLFEAFTQAGQVVSVRVCRDMTTRRSLGYGYVNYATPQDASRALNELN 107

Query: 88  MIKLYGKPIRVNKASQDKKSLDVG-ANLFIGNLDPDVDEKLLYDTFSAFGVIVTNPKIMR 147
            + L G+ IRV  + +D      G  N+FI NLD  +D K L++TFSAFG I+ + K+  
Sbjct: 108 FMALNGRAIRVMYSVRDPSLRKSGVGNIFIKNLDKSIDHKALHETFSAFGPIL-SCKVAV 167

Query: 148 DPDTGNSRGFGFISYDSFEASDAAIEAMNGQYLCNRQITVS---YAYKKDTKGER 199
           DP +G S+G+GF+ YD+ EA+  AI+ +NG  L ++Q+ V    +  ++D  GE+
Sbjct: 168 DP-SGQSKGYGFVQYDTDEAAQGAIDKLNGMLLNDKQVYVGPFVHKLQRDPSGEK 220

[Close]

BLAST of CmaCh06G016120 vs. TAIR 10
Match: AT1G49760.2 (poly(A) binding protein 8 )

HSP 1 Score: 131.3 bits (329), Expect = 1.6e-30
Identity = 71/175 (40.57%), Postives = 109/175 (62.29%), Query Frame = 0


Query: 28  YVGNLDPQVSEELLWELFVQAGPVVNVYVPKDRVTNLHQGYGFIEFRSEEDADYAIKVLN 87
           YVG+LD  V++  L+E F QAG VV+V V +D  T    GYG++ + + +DA  A+  LN
Sbjct: 48  YVGDLDATVTDSQLFEAFTQAGQVVSVRVCRDMTTRRSLGYGYVNYATPQDASRALNELN 107

Query: 88  MIKLYGKPIRVNKASQDKKSLDVG-ANLFIGNLDPDVDEKLLYDTFSAFGVIVTNPKIMR 147
            + L G+ IRV  + +D      G  N+FI NLD  +D K L++TFSAFG I+ + K+  
Sbjct: 108 FMALNGRAIRVMYSVRDPSLRKSGVGNIFIKNLDKSIDHKALHETFSAFGPIL-SCKVAV 167

Query: 148 DPDTGNSRGFGFISYDSFEASDAAIEAMNGQYLCNRQITVS---YAYKKDTKGER 199
           DP +G S+G+GF+ YD+ EA+  AI+ +NG  L ++Q+ V    +  ++D  GE+
Sbjct: 168 DP-SGQSKGYGFVQYDTDEAAQGAIDKLNGMLLNDKQVYVGPFVHKLQRDPSGEK 220

[Close]

BLAST of CmaCh06G016120 vs. TAIR 10
Match: AT1G22760.1 (poly(A) binding protein 3 )

HSP 1 Score: 123.6 bits (309), Expect = 3.4e-28
Identity = 65/189 (34.39%), Postives = 114/189 (60.32%), Query Frame = 0


Query: 18  APERNQDATAYVGNLDPQVSEELLWELFVQAGPVVNVYVPKDRVTNLHQGYGFIEFRSEE 77
           A + + +++ Y G+LDP+V+E  L++LF     VV+V V +D+      GY +I F +  
Sbjct: 42  ADQTHPNSSLYAGDLDPKVTEAHLFDLFKHVANVVSVRVCRDQ-NRRSLGYAYINFSNPN 101

Query: 78  DADYAIKVLNMIKLYGKPIRVNKASQDKKS-LDVGANLFIGNLDPDVDEKLLYDTFSAFG 137
           DA  A++ LN   L+ +PIR+  +++D  + L    N+FI NLD  +D K L++TFS+FG
Sbjct: 102 DAYRAMEALNYTPLFDRPIRIMLSNRDPSTRLSGKGNIFIKNLDASIDNKALFETFSSFG 161

Query: 138 VIVTNPKIMRDPDTGNSRGFGFISYDSFEASDAAIEAMNGQYLCNRQITVSYAYKKDTKG 197
            I+ + K+  D  TG S+G+GF+ ++  E++ AAI+ +NG  + ++Q+ V +  ++  + 
Sbjct: 162 TIL-SCKVAMDV-TGRSKGYGFVQFEKEESAQAAIDKLNGMLMNDKQVFVGHFIRRQERA 221

Query: 198 ERHGTPAER 206
               TP  R
Sbjct: 222 RDENTPTPR 227

The following BLAST results are available for this feature:

BLAST of CmaCh06G016120 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q15427	7.1e-92	54.23	Splicing factor 3B subunit 4 OS=Homo sapiens OX=9606 GN=SF3B4 PE=1 SV=1	[more]
Q8QZY9	7.1e-92	54.23	Splicing factor 3B subunit 4 OS=Mus musculus OX=10090 GN=Sf3b4 PE=1 SV=1	[more]
Q6AYL5	7.1e-92	54.23	Splicing factor 3B subunit 4 OS=Rattus norvegicus OX=10116 GN=Sf3b4 PE=2 SV=1	[more]
Q09442	6.3e-88	55.06	Splicing factor 3B subunit 4 OS=Caenorhabditis elegans OX=6239 GN=sap-49 PE=1 SV...	[more]
O14102	5.3e-63	46.32	Spliceosome-associated protein 49 OS=Schizosaccharomyces pombe (strain 972 / ATC...	[more]

BLAST of CmaCh06G016120 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G18510.1	1.3e-136	73.30	RNA-binding (RRM/RBD/RNP motifs) family protein	[more]
AT4G34110.1	1.2e-30	44.51	poly(A) binding protein 2	[more]
AT1G49760.1	1.6e-30	40.57	poly(A) binding protein 8	[more]
AT1G49760.2	1.6e-30	40.57	poly(A) binding protein 8	[more]
AT1G22760.1	3.4e-28	34.39	poly(A) binding protein 3	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000504	RNA recognition motif domain	SMART	SM00360	rrm1_1	coord: 26..99 e-value: 2.2E-25 score: 100.3 coord: 113..187 e-value: 1.4E-24 score: 97.7
IPR000504	RNA recognition motif domain	PFAM	PF00076	RRM_1	coord: 28..97 e-value: 2.0E-19 score: 69.2 coord: 114..184 e-value: 5.0E-20 score: 71.1
IPR000504	RNA recognition motif domain	PROSITE	PS50102	RRM	coord: 25..103 score: 18.563835
IPR000504	RNA recognition motif domain	PROSITE	PS50102	RRM	coord: 112..191 score: 17.267626
IPR012677	Nucleotide-binding alpha-beta plait domain superfamily	GENE3D	3.30.70.330		coord: 17..106 e-value: 2.2E-26 score: 93.9
IPR012677	Nucleotide-binding alpha-beta plait domain superfamily	GENE3D	3.30.70.330		coord: 107..204 e-value: 4.0E-26 score: 93.6
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 280..294
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 301..351
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 361..382
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 256..279
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 248..382
None	No IPR available	PANTHER	PTHR15241	TRANSFORMER-2-RELATED	coord: 1..359
None	No IPR available	PANTHER	PTHR15241:SF330	SPLICING FACTOR 3B SUBUNIT 4	coord: 1..359
IPR034158	SF3B4, RNA recognition motif 1	CDD	cd12334	RRM1_SF3B4	coord: 28..100 e-value: 5.38922E-55 score: 173.549
IPR034159	SF3B4, RNA recognition motif 2	CDD	cd12335	RRM2_SF3B4	coord: 111..193 e-value: 5.17619E-63 score: 194.436
IPR035979	RNA-binding domain superfamily	SUPERFAMILY	54928	RNA-binding domain, RBD	coord: 25..194

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmaCh06G016120.1	CmaCh06G016120.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0048026	positive regulation of mRNA splicing, via spliceosome
cellular_component	GO:0005689	U12-type spliceosomal complex
molecular_function	GO:0003723	RNA binding
molecular_function	GO:0003676	nucleic acid binding