CmaCh06G015140 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh06G015140
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
DescriptionDihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex
LocationCma_Chr06: 9578434 .. 9579660 (+)
RNA-Seq ExpressionCmaCh06G015140
SyntenyCmaCh06G015140
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCGATTCTAATGATCAGCAGGAGGAAGATTTTTCCGACGAGTGCTTGGGCCGCCGGTCGTCGGTTACTCTTTCCGGTGGCCTTCCAAGGTCCCTCTGCTTCAATCTCTCAGTTGCCGTCGGAGCAGACGCCCAAATCGCTTCCAGAGCCATATCGTACACTTGCATGCCCTAGTTTCTGTAGANTTTTTTTTTTTTATTTCAAGCCCAATTTGATTGGGCTATAAATTTACTAGATCAAATGATTATTCGTAGTAGTTGGATGAAAGTCCCACGTCGGCTAATTTAGGAAATGATCCTGAGTTTATAATATAATCAAGAAATACTATCTCCATTGGTACGAGACCTTTTGGGGAAGCCCAAAGCAAAGCCACGAGAGATTATGCTCAAAGTGGACAATATCATACTGTTGCGGAGAGTCGTGTTCGTCTAACATAGTATAAGAGTTATGCCCTAAACTTAGCCATGTCAATAGATCGTCTAACATGCCTTGTCAATAGAATCCTCAAATGTCGAACAAATGACTCCAAAAGAAAAAGAAAAGGTCGAAGGCATAGTAAAAAATGATGTACTTTGTTCGAGGGGAGGTGTTGGATGTAATTTAGGGAATGAACATGGGTTTATAATATAATCAAGGAATACTGCCTCAATTAAGTACTTTGTTCGAGGGGAGGTGTTGGATGAAAGTCCCACATCGGCTAATTTTATAATAGGCCTTTTAAGGGAAGCTCAAAGCAAAGCCACTTGTTCGTGGGGAGGTGTTGGATGAAAGTCCCACATCGGCTAATTTAGGAAATGATCATGAGTTTATAATAGGCATTTTGAGGGAAGCTCAAAGCAAAGCCACAAGAGATTATGCTCAAAGTGGACAATATCATACCATTGTGGAGAGTCGTGTTCGTCTAACAGTAGCTAGGAGGTTTTTTTTTAGTGTACTGACATTTTTCTCGAGAATTAGGTCACGCGTGCCTCCTTAAGAGGTTTAAATCATACCAAATTATTCGTACCACCTTAGGAGGTAGATCATAGGATCAACATCGACTTCGTAGGCCAAGACTGGCAAATTCTTATATTCTTCTTTGCTTCATTGTCCGATCTTTTTATGTTGACCCAAATAATGTCCTATATGGAGATTCTTCTCGTAAGTTTGACCTCTCTTTTCTATATTAACTCGGGTTTATTTATTTTTTTGGTTTTAAGTTATTTCTAGCCACCACTCTAAATGA

mRNA sequence

ATGTCGATTCTAATGATCAGCAGGAGGAAGATTTTTCCGACGAGTGCTTGGGCCGCCGGTCGTCGGTTACTCTTTCCGGTGGCCTTCCAAGGTCCCTCTGCTTCAATCTCTCAGTTGCCGTCGGAGCAGACGCCCAAATCGCTTCCAGAGCCATATCGTACACTTGCATGCCCTATTATTTCTAGCCACCACTCTAAATGA

Coding sequence (CDS)

ATGTCGATTCTAATGATCAGCAGGAGGAAGATTTTTCCGACGAGTGCTTGGGCCGCCGGTCGTCGGTTACTCTTTCCGGTGGCCTTCCAAGGTCCCTCTGCTTCAATCTCTCAGTTGCCGTCGGAGCAGACGCCCAAATCGCTTCCAGAGCCATATCGTACACTTGCATGCCCTATTATTTCTAGCCACCACTCTAAATGA

Protein sequence

MSILMISRRKIFPTSAWAAGRRLLFPVAFQGPSASISQLPSEQTPKSLPEPYRTLACPIISSHHSK
Homology
BLAST of CmaCh06G015140 vs. ExPASy TrEMBL
Match: A0A6J1I8C5 (Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex OS=Cucurbita maxima OX=3661 GN=LOC111472314 PE=3 SV=1)

HSP 1 Score: 117.5 bits (293), Expect = 2.2e-23
Identity = 58/58 (100.00%), Postives = 58/58 (100.00%), Query Frame = 0

Query: 1  MSILMISRRKIFPTSAWAAGRRLLFPVAFQGPSASISQLPSEQTPKSLPEPYRTLACP 59
          MSILMISRRKIFPTSAWAAGRRLLFPVAFQGPSASISQLPSEQTPKSLPEPYRTLACP
Sbjct: 1  MSILMISRRKIFPTSAWAAGRRLLFPVAFQGPSASISQLPSEQTPKSLPEPYRTLACP 58

BLAST of CmaCh06G015140 vs. ExPASy TrEMBL
Match: A0A6J1G741 (Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex OS=Cucurbita moschata OX=3662 GN=LOC111451360 PE=3 SV=1)

HSP 1 Score: 110.5 bits (275), Expect = 2.6e-21
Identity = 55/58 (94.83%), Postives = 56/58 (96.55%), Query Frame = 0

Query: 1  MSILMISRRKIFPTSAWAAGRRLLFPVAFQGPSASISQLPSEQTPKSLPEPYRTLACP 59
          MSILMISRRKIFPTS+WAAG RLLFPVAFQGPSASISQLPS QTPKSLPEPYRTLACP
Sbjct: 1  MSILMISRRKIFPTSSWAAGCRLLFPVAFQGPSASISQLPSVQTPKSLPEPYRTLACP 58

BLAST of CmaCh06G015140 vs. ExPASy TrEMBL
Match: A0A6J1C9A1 (Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex OS=Momordica charantia OX=3673 GN=LOC111009145 PE=3 SV=1)

HSP 1 Score: 76.3 bits (186), Expect = 5.5e-11
Identity = 40/62 (64.52%), Postives = 45/62 (72.58%), Query Frame = 0

Query: 1  MSILMISRRKIFPTSAWAAGRRLLFPVAFQGPSASISQLPSEQTPKSLPEPYRTLACPII 60
          MS++MI RRKI P SAWAAGRRLL P A QG   S+ QLP EQTPKS+PEP   LAC   
Sbjct: 1  MSMIMIGRRKISPGSAWAAGRRLLSPFASQGSFPSLFQLPLEQTPKSIPEPSPPLACSRF 60

Query: 61 SS 63
          S+
Sbjct: 61 ST 62

BLAST of CmaCh06G015140 vs. ExPASy TrEMBL
Match: A0A0A0L5U6 (Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex OS=Cucumis sativus OX=3659 GN=Csa_3G164470 PE=3 SV=1)

HSP 1 Score: 71.6 bits (174), Expect = 1.4e-09
Identity = 36/58 (62.07%), Postives = 41/58 (70.69%), Query Frame = 0

Query: 1  MSILMISRRKIFPTSAWAAGRRLLFPVAFQGPSASISQLPSEQTPKSLPEPYRTLACP 59
          MSIL I RR+I  T+AWA GRRLL P   Q PS SISQ PS+QTP+SLP P+  L  P
Sbjct: 1  MSILTIGRRRISSTNAWATGRRLLSPFTSQSPSPSISQFPSQQTPESLPHPFPPLGSP 58

BLAST of CmaCh06G015140 vs. ExPASy TrEMBL
Match: A0A5D3CXV6 (Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold209G001180 PE=3 SV=1)

HSP 1 Score: 65.9 bits (159), Expect = 7.4e-08
Identity = 34/55 (61.82%), Postives = 39/55 (70.91%), Query Frame = 0

Query: 1  MSILMISRRKIFPTSAWAAGRRLLFPVAFQGPSASISQLPSEQTPKSLPEPYRTL 56
          MSIL I RR+I P +AWAAG RLL P   Q PSASI Q  S+QTP+SLP P+  L
Sbjct: 1  MSILTIGRRRISPRNAWAAGCRLLSPFTSQSPSASIPQFSSQQTPESLPHPFPPL 55

BLAST of CmaCh06G015140 vs. NCBI nr
Match: XP_022973737.1 (lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex, mitochondrial [Cucurbita maxima])

HSP 1 Score: 117.5 bits (293), Expect = 4.5e-23
Identity = 58/58 (100.00%), Postives = 58/58 (100.00%), Query Frame = 0

Query: 1  MSILMISRRKIFPTSAWAAGRRLLFPVAFQGPSASISQLPSEQTPKSLPEPYRTLACP 59
          MSILMISRRKIFPTSAWAAGRRLLFPVAFQGPSASISQLPSEQTPKSLPEPYRTLACP
Sbjct: 1  MSILMISRRKIFPTSAWAAGRRLLFPVAFQGPSASISQLPSEQTPKSLPEPYRTLACP 58

BLAST of CmaCh06G015140 vs. NCBI nr
Match: XP_022947515.1 (lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex, mitochondrial [Cucurbita moschata] >KAG7028992.1 Lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex, mitochondrial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 110.5 bits (275), Expect = 5.4e-21
Identity = 55/58 (94.83%), Postives = 56/58 (96.55%), Query Frame = 0

Query: 1  MSILMISRRKIFPTSAWAAGRRLLFPVAFQGPSASISQLPSEQTPKSLPEPYRTLACP 59
          MSILMISRRKIFPTS+WAAG RLLFPVAFQGPSASISQLPS QTPKSLPEPYRTLACP
Sbjct: 1  MSILMISRRKIFPTSSWAAGCRLLFPVAFQGPSASISQLPSVQTPKSLPEPYRTLACP 58

BLAST of CmaCh06G015140 vs. NCBI nr
Match: XP_023540739.1 (lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex, mitochondrial [Cucurbita pepo subsp. pepo])

HSP 1 Score: 106.3 bits (264), Expect = 1.0e-19
Identity = 54/58 (93.10%), Postives = 54/58 (93.10%), Query Frame = 0

Query: 1  MSILMISRRKIFPTSAWAAGRRLLFPVAFQGPSASISQLPSEQTPKSLPEPYRTLACP 59
          MSILMISRRKIFP SAWAAGRRLLFPVAF GPSASISQLPSEQTPKSL E YRTLACP
Sbjct: 1  MSILMISRRKIFPMSAWAAGRRLLFPVAFLGPSASISQLPSEQTPKSLSETYRTLACP 58

BLAST of CmaCh06G015140 vs. NCBI nr
Match: KAG6597534.1 (Lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 104.4 bits (259), Expect = 3.9e-19
Identity = 51/54 (94.44%), Postives = 52/54 (96.30%), Query Frame = 0

Query: 5  MISRRKIFPTSAWAAGRRLLFPVAFQGPSASISQLPSEQTPKSLPEPYRTLACP 59
          MISRRKIFPTS+WAAG RLLFPVAFQGPSASISQLPS QTPKSLPEPYRTLACP
Sbjct: 1  MISRRKIFPTSSWAAGCRLLFPVAFQGPSASISQLPSVQTPKSLPEPYRTLACP 54

BLAST of CmaCh06G015140 vs. NCBI nr
Match: XP_038883373.1 (lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex, mitochondrial isoform X1 [Benincasa hispida])

HSP 1 Score: 79.0 bits (193), Expect = 1.8e-11
Identity = 39/56 (69.64%), Postives = 45/56 (80.36%), Query Frame = 0

Query: 1  MSILMISRRKIFPTSAWAAGRRLLFPVAFQGPSASISQLPSEQTPKSLPEPYRTLA 57
          MSIL I RR+I P +AWA GRRLL P  +QG SASISQLPS+QTPKSLP+P+  LA
Sbjct: 1  MSILTIGRRRISPRTAWATGRRLLSPFTYQGSSASISQLPSDQTPKSLPQPFPQLA 56

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A6J1I8C52.2e-23100.00Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex O... [more]
A0A6J1G7412.6e-2194.83Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex O... [more]
A0A6J1C9A15.5e-1164.52Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex O... [more]
A0A0A0L5U61.4e-0962.07Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex O... [more]
A0A5D3CXV67.4e-0861.82Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex O... [more]
Match NameE-valueIdentityDescription
XP_022973737.14.5e-23100.00lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogen... [more]
XP_022947515.15.4e-2194.83lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogen... [more]
XP_023540739.11.0e-1993.10lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogen... [more]
KAG6597534.13.9e-1994.44Lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogen... [more]
XP_038883373.11.8e-1169.64lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogen... [more]
Match NameE-valueIdentityDescription
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh06G015140.1CmaCh06G015140.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0005739 mitochondrion
molecular_function GO:0016407 acetyltransferase activity
molecular_function GO:0031405 lipoic acid binding