CmaCh06G014330 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh06G014330
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Descriptionheavy metal-associated isoprenylated plant protein 32
LocationCma_Chr06: 9207153 .. 9208802 (-)
RNA-Seq ExpressionCmaCh06G014330
SyntenyCmaCh06G014330
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAGTAAAGAAGAGTTTCTGAAGATCCAGGTGCTTTGTTTACTCGACATGTTCTGTTTTTCTGTAATGTTGTAGCTACTGTCTGTTCATTTTGTTCTGTTTTTGTGTCGTTTTGAGTGGTTCTGCAGAAATGTGTGTTGAAGGTCAATATTCACTGCGATGGATGTAAGCAAAAGGTGAAGAAAATCTTGCAGAAAATCGATGGTACTCTATTTTCTTATGATGCTTCTGTCGGCTCTGTTTTTTACTTGTCTTTGAGTTTGATGGGGTTTGTGTGTGTGTGTGTGTTTTTTAGGGGTTTTCACTACCGAGATAGATGCAGAACAGGGGAAGGTTACAGTCACTGGAAATGTCGACGCCGCCGTACTCATCAAGAAGCTGTCTAAATCCGGCAAACATGCAGAGATTTGGGGCGGGCAACCGGCGAACAACAACAAGAATCAGCAGAACAACATAGCTAATCAGATGAAAAACATGCAAATTGACAATGCCAAAGGGGGGAACAACAAGGGTCAGAACCAGAAGGGTGCGGCTGCTAATGGAGGAAACAACCAGCCGAAAGGCGGCGGACAGGGACAGGGGCTGCCACCGGTGCAGCTTCAGCAGCTACAACAGATGAAAGGGTTTCAAGATCTGAAGCTTCCACCCCAATTTAAGGGGTTGAAACTGCCTGTTAAAGATCAAAACGCGAATCTTCCCAAGGGTGGGAAGTTTAATTTGCACGAAGGTGATGACCTGAGCGATGAGAATGATGAGGATGATTATGACGATGAGGATGATTATGATGATGATGATGAACTTGATGATGATTTGGGTAATCTTCATCTTCCTCCTGCGAGTAAGATGAAACCCATGATGGGAAATGGCCAGATTCCGAAAATGATGATGAATGGAAACCTTCCCCCGGTTGTGAATGGTCCGGCCGGCGGTGGTAATGCCAAGAAAGGCGGCGGAGCTGTGCCGGTTCAGGGTAATAACAATGGGAAGAATGTTACCGGCGGAGGAGGTAAAGGAAATGGTGGCGGCGGCGGTGGAGGTGGGAGTAACCAAAAGCAGGGCGGTGGAGGTGGGAGTAACCAAAAGCAGGGCGGCGGCTGCGGCGGCGGTGTGAGCAATGGCAAAAACGGCAACGGCGGTGGAAATGGTAGTAGTAACGGGGTGAACGGCGGTCAAAAGATGACCGGTGGGTCAAATGGAATGGGCCATGATTTACAGAGCTTCATCGGTGCTCATGGATTGGGAATGTCAAATTTGGGTCAAATGGGTGACCGGCCGATGGGTCAAATGAACAATCTTCCAGCGGTGCAAGGTCTTCCCGCATCAGCGGCGGCAGCCATGAACGGTGGAGGCAGCGCCGGGAGGTTTATACCGGACAGCATTTCCACAAACCCCTACCACCACCACCACCAGCAACAGCAACAGCAACAGCAACAACACATGGCCCAAATGATGAACCAACAACAACACGCGCTTGGGCACCAACCAATGATGTACGCACGGCCACCGCCGGCCGTAAATTACGTGCCACCGCCGTACCCATACCCATATCCATACCCACCACCGTACCACCACCCATATCCACCGCCGCAGCAGCCAGAACCCTACACGTATTTCAGCGACGAGAATCCGTCAAGTTGTAACATCATGTGA

mRNA sequence

ATGAGTAAAGAAGAGTTTCTGAAGATCCAGAAATGTGTGTTGAAGGTCAATATTCACTGCGATGGATGTAAGCAAAAGGTGAAGAAAATCTTGCAGAAAATCGATGGGGTTTTCACTACCGAGATAGATGCAGAACAGGGGAAGGTTACAGTCACTGGAAATGTCGACGCCGCCGTACTCATCAAGAAGCTGTCTAAATCCGGCAAACATGCAGAGATTTGGGGCGGGCAACCGGCGAACAACAACAAGAATCAGCAGAACAACATAGCTAATCAGATGAAAAACATGCAAATTGACAATGCCAAAGGGGGGAACAACAAGGGTCAGAACCAGAAGGGTGCGGCTGCTAATGGAGGAAACAACCAGCCGAAAGGCGGCGGACAGGGACAGGGGCTGCCACCGGTGCAGCTTCAGCAGCTACAACAGATGAAAGGGTTTCAAGATCTGAAGCTTCCACCCCAATTTAAGGGGTTGAAACTGCCTGTTAAAGATCAAAACGCGAATCTTCCCAAGGGTGGGAAGTTTAATTTGCACGAAGGTGATGACCTGAGCGATGAGAATGATGAGGATGATTATGACGATGAGGATGATTATGATGATGATGATGAACTTGATGATGATTTGGGTAATCTTCATCTTCCTCCTGCGAGTAAGATGAAACCCATGATGGGAAATGGCCAGATTCCGAAAATGATGATGAATGGAAACCTTCCCCCGGTTGTGAATGGTCCGGCCGGCGGTGGTAATGCCAAGAAAGGCGGCGGAGCTGTGCCGGTTCAGGGTAATAACAATGGGAAGAATGTTACCGGCGGAGGAGGTAAAGGAAATGGTGGCGGCGGCGGTGGAGGTGGGAGTAACCAAAAGCAGGGCGGTGGAGGTGGGAGTAACCAAAAGCAGGGCGGCGGCTGCGGCGGCGGTGTGAGCAATGGCAAAAACGGCAACGGCGGTGGAAATGGTAGTAGTAACGGGGTGAACGGCGGTCAAAAGATGACCGGTGGGTCAAATGGAATGGGCCATGATTTACAGAGCTTCATCGGTGCTCATGGATTGGGAATGTCAAATTTGGGTCAAATGGGTGACCGGCCGATGGGTCAAATGAACAATCTTCCAGCGGTGCAAGGTCTTCCCGCATCAGCGGCGGCAGCCATGAACGGTGGAGGCAGCGCCGGGAGGTTTATACCGGACAGCATTTCCACAAACCCCTACCACCACCACCACCAGCAACAGCAACAGCAACAGCAACAACACATGGCCCAAATGATGAACCAACAACAACACGCGCTTGGGCACCAACCAATGATGTACGCACGGCCACCGCCGGCCGTAAATTACGTGCCACCGCCGTACCCATACCCATATCCATACCCACCACCGTACCACCACCCATATCCACCGCCGCAGCAGCCAGAACCCTACACGTATTTCAGCGACGAGAATCCGTCAAGTTGTAACATCATGTGA

Coding sequence (CDS)

ATGAGTAAAGAAGAGTTTCTGAAGATCCAGAAATGTGTGTTGAAGGTCAATATTCACTGCGATGGATGTAAGCAAAAGGTGAAGAAAATCTTGCAGAAAATCGATGGGGTTTTCACTACCGAGATAGATGCAGAACAGGGGAAGGTTACAGTCACTGGAAATGTCGACGCCGCCGTACTCATCAAGAAGCTGTCTAAATCCGGCAAACATGCAGAGATTTGGGGCGGGCAACCGGCGAACAACAACAAGAATCAGCAGAACAACATAGCTAATCAGATGAAAAACATGCAAATTGACAATGCCAAAGGGGGGAACAACAAGGGTCAGAACCAGAAGGGTGCGGCTGCTAATGGAGGAAACAACCAGCCGAAAGGCGGCGGACAGGGACAGGGGCTGCCACCGGTGCAGCTTCAGCAGCTACAACAGATGAAAGGGTTTCAAGATCTGAAGCTTCCACCCCAATTTAAGGGGTTGAAACTGCCTGTTAAAGATCAAAACGCGAATCTTCCCAAGGGTGGGAAGTTTAATTTGCACGAAGGTGATGACCTGAGCGATGAGAATGATGAGGATGATTATGACGATGAGGATGATTATGATGATGATGATGAACTTGATGATGATTTGGGTAATCTTCATCTTCCTCCTGCGAGTAAGATGAAACCCATGATGGGAAATGGCCAGATTCCGAAAATGATGATGAATGGAAACCTTCCCCCGGTTGTGAATGGTCCGGCCGGCGGTGGTAATGCCAAGAAAGGCGGCGGAGCTGTGCCGGTTCAGGGTAATAACAATGGGAAGAATGTTACCGGCGGAGGAGGTAAAGGAAATGGTGGCGGCGGCGGTGGAGGTGGGAGTAACCAAAAGCAGGGCGGTGGAGGTGGGAGTAACCAAAAGCAGGGCGGCGGCTGCGGCGGCGGTGTGAGCAATGGCAAAAACGGCAACGGCGGTGGAAATGGTAGTAGTAACGGGGTGAACGGCGGTCAAAAGATGACCGGTGGGTCAAATGGAATGGGCCATGATTTACAGAGCTTCATCGGTGCTCATGGATTGGGAATGTCAAATTTGGGTCAAATGGGTGACCGGCCGATGGGTCAAATGAACAATCTTCCAGCGGTGCAAGGTCTTCCCGCATCAGCGGCGGCAGCCATGAACGGTGGAGGCAGCGCCGGGAGGTTTATACCGGACAGCATTTCCACAAACCCCTACCACCACCACCACCAGCAACAGCAACAGCAACAGCAACAACACATGGCCCAAATGATGAACCAACAACAACACGCGCTTGGGCACCAACCAATGATGTACGCACGGCCACCGCCGGCCGTAAATTACGTGCCACCGCCGTACCCATACCCATATCCATACCCACCACCGTACCACCACCCATATCCACCGCCGCAGCAGCCAGAACCCTACACGTATTTCAGCGACGAGAATCCGTCAAGTTGTAACATCATGTGA

Protein sequence

MSKEEFLKIQKCVLKVNIHCDGCKQKVKKILQKIDGVFTTEIDAEQGKVTVTGNVDAAVLIKKLSKSGKHAEIWGGQPANNNKNQQNNIANQMKNMQIDNAKGGNNKGQNQKGAAANGGNNQPKGGGQGQGLPPVQLQQLQQMKGFQDLKLPPQFKGLKLPVKDQNANLPKGGKFNLHEGDDLSDENDEDDYDDEDDYDDDDELDDDLGNLHLPPASKMKPMMGNGQIPKMMMNGNLPPVVNGPAGGGNAKKGGGAVPVQGNNNGKNVTGGGGKGNGGGGGGGGSNQKQGGGGGSNQKQGGGCGGGVSNGKNGNGGGNGSSNGVNGGQKMTGGSNGMGHDLQSFIGAHGLGMSNLGQMGDRPMGQMNNLPAVQGLPASAAAAMNGGGSAGRFIPDSISTNPYHHHHQQQQQQQQQHMAQMMNQQQHALGHQPMMYARPPPAVNYVPPPYPYPYPYPPPYHHPYPPPQQPEPYTYFSDENPSSCNIM
Homology
BLAST of CmaCh06G014330 vs. ExPASy Swiss-Prot
Match: Q9M8K5 (Heavy metal-associated isoprenylated plant protein 32 OS=Arabidopsis thaliana OX=3702 GN=HIPP32 PE=2 SV=1)

HSP 1 Score: 307.4 bits (786), Expect = 2.9e-82
Identity = 274/514 (53.31%), Postives = 321/514 (62.45%), Query Frame = 0

Query: 1   MSKEEFLKIQKCVLKVNIHCDGCKQKVKKILQKIDGVFTTEIDAEQGKVTVTGNVDAAVL 60
           MSKEEF+KIQ CVLKVNIHCDGCKQKVKKILQKI+GVFTT+ID+EQGKVTV+G+VD +VL
Sbjct: 1   MSKEEFMKIQTCVLKVNIHCDGCKQKVKKILQKIEGVFTTKIDSEQGKVTVSGSVDPSVL 60

Query: 61  IKKLSKSGKHAEIWGGQPANNNKNQQNNIANQMKNMQIDNAK----GGNNKGQNQKGAAA 120
           IKKL+KSGKHAEIWG    NNN N Q+ +ANQ K MQID+ K    GG N   N+KG   
Sbjct: 61  IKKLAKSGKHAEIWGAPKGNNNPN-QSQMANQFKGMQIDHGKAGGGGGGNNNNNKKGQKN 120

Query: 121 NGGNNQPKGGG------QGQGLPP---VQLQQLQQMKGFQDLKLPPQFKGLKLPVKDQNA 180
            GG     GGG       GQ L P    QLQQLQ+MKGFQDLKLPPQ KG     K+QN 
Sbjct: 121 GGGGGGGGGGGNSNAPKMGQQLNPQHLQQLQQLQKMKGFQDLKLPPQLKGSVPVNKNQN- 180

Query: 181 NLPKGGKFNLHEGDDLSDENDEDDYDDEDDYDDDDELDDDLGNLHLPPASKMKP---MMG 240
              KG KF++ E DD  D++  D++DDE   DDDDE DD+  +L L P++KMKP   MM 
Sbjct: 181 --QKGVKFDVPEDDD--DDDFSDEFDDEFTDDDDDEFDDEFDDLPL-PSNKMKPNMTMMP 240

Query: 241 NGQIPKMMMNGNLPPVVNGPAGGGNAKKGGGAVPVQGNNNGKNVTGGGGKGNGGGGGGGG 300
           N Q  +MMMN       N    GG AK GG   P  G         GGGKG GGG  GG 
Sbjct: 241 NAQ--QMMMNAQ----KNANLAGGPAKNGGKGAPAAGGGG-----AGGGKGAGGGAKGGP 300

Query: 301 SNQKQGG---GGGSNQKQG--GGCGGGVSNGKNGNGGGNGSSNGVNGGQKMTGGSNGMGH 360
            NQ QGG   GGG + + G  GG GGG + GK GNGGG   + GV+GG +  GG      
Sbjct: 301 GNQNQGGGKNGGGGHPQDGKNGGGGGGPNAGKKGNGGGGPMAGGVSGGFRPMGGG----- 360

Query: 361 DLQSFIGAHGLGMSNLGQMGDRPMGQMNNLPAVQGLPASAAAAMNGGGSAGRFIPDSIST 420
                 G   + M   GQMG    G M N+PAVQGLPA+      GG   G F    I  
Sbjct: 361 ------GPQNMSMPMGGQMG--MGGPMGNMPAVQGLPATGP----GGAPQGYFQGAGIDP 420

Query: 421 NPYHHHHQQQQQQQQQHMAQMMNQQQHALGH---QPMMYARPPPAVNYVPP---PYPYPY 480
                   QQQQQQQQ++A +MNQQ+ A+G+   QPMMYARPPPAVNY+PP    YP P+
Sbjct: 421 -----MQMQQQQQQQQYLAAVMNQQR-AMGNERFQPMMYARPPPAVNYMPPNPHQYPNPH 473

Query: 481 PYPPPYHHPYPPPQQPEPYTY-FSDENPSSCNIM 487
           PYP PY +PYPPP   + Y++ FSDEN SSC+IM
Sbjct: 481 PYPYPYPYPYPPPYGNDQYSHAFSDENTSSCDIM 473

BLAST of CmaCh06G014330 vs. ExPASy Swiss-Prot
Match: F4JZL7 (Heavy metal-associated isoprenylated plant protein 33 OS=Arabidopsis thaliana OX=3702 GN=HIPP33 PE=2 SV=1)

HSP 1 Score: 301.2 bits (770), Expect = 2.1e-80
Identity = 292/600 (48.67%), Postives = 333/600 (55.50%), Query Frame = 0

Query: 1   MSKEEFLKIQKCVLKVNIHCDGCKQKVKKILQKIDGVFTTEIDAEQGKVTVTGNVDAAVL 60
           MSKEEF+KIQ CVLKVNIHCDGCKQKVKKILQKI+GVFTT+IDAE GKVTV+GNVD +VL
Sbjct: 1   MSKEEFMKIQTCVLKVNIHCDGCKQKVKKILQKIEGVFTTKIDAELGKVTVSGNVDPSVL 60

Query: 61  IKKLSKSGKHAEIWGGQPANNNKNQ-QNNIANQMKNMQIDN-AKGG---------NNKGQ 120
           IKKL KSGKHAEIWG     +N NQ Q N+ANQ K MQID+  KGG         NNKGQ
Sbjct: 61  IKKLLKSGKHAEIWGAPKGGSNNNQNQPNLANQFKAMQIDHGGKGGGGGGGGPANNNKGQ 120

Query: 121 NQKGAAANGGNNQPKGGGQGQGLPP--------------------VQLQQLQQMKGFQDL 180
              G    GG     GGG G G PP                     QLQQLQQMKGFQDL
Sbjct: 121 KIGGGGGGGGG----GGGGGGGGPPKMVIPQLTPQQMQQLNPQQLQQLQQLQQMKGFQDL 180

Query: 181 KLPPQFKGLKLP-----VKDQNANLP-----KGGKFNLHEGDDLSDENDE-DDYDDEDDY 240
           KLPPQ KG   P       ++N  +P     K  KFN+ + DD  D +DE DD  DEDD 
Sbjct: 181 KLPPQLKGGPGPGPGSVPMNKNPQMPNNPNQKAVKFNVPDDDDEEDFSDEFDDEFDEDDD 240

Query: 241 DDDDELDDDLGNLHLPPASKMKPMMG-------NGQIPKMMM-----------NGNLP-- 300
           + DD+L+DD  + H PP +KMKPMMG       N  +P MMM           NG  P  
Sbjct: 241 EFDDDLEDDEFDDHPPPPNKMKPMMGGGNMIMPNNMMPNMMMPNAQQMLNAHKNGGGPGP 300

Query: 301 ------------PVVNGPAGGGNAKKGGGAVPVQGNNNGKNVTGGGGKGNGGGGGGGGSN 360
                       PV  G  GGG   K GG     GN   +N  GGGGK  G GGGG   +
Sbjct: 301 AGGKIEGKGMPFPVQMGGGGGGPGGKKGGPGGGGGNMGNQN-QGGGGKNGGKGGGGHPLD 360

Query: 361 QKQGGGGGS-NQKQGGG---CGGGVSNGKNGNGGGNGS-----SNGVNGGQKMTGGSNGM 420
            K GGGGG  N  +GGG     GG + GK G GGG G      S G+  G +  GG  G 
Sbjct: 361 GKMGGGGGGPNGNKGGGGVQMNGGPNGGKKGGGGGGGGGGGPMSGGLPPGFRPMGGGGGG 420

Query: 421 GHDLQSFI----GAHGLGMSNLGQMGDRPMGQMNNLPAVQGLPASAAAAMNGGGSAGRFI 480
           G   QS      GA G  M +L QMG  P    NN+ AVQGLPA       GGG +    
Sbjct: 421 GGGPQSMSMPMGGAMGGPMGSLPQMGGGPGPMSNNMQAVQGLPAMGPGGGGGGGPSAEAP 480

Query: 481 P---------------DSISTNPYHHHHQQQQQQQQQHMAQMMNQQQHALGH---QPMMY 487
           P               DS+  NPY    QQQQQQQQQ++A +MNQQ+ ++G+   QPMMY
Sbjct: 481 PGYFQGQVSGNGGGGQDSMPGNPY--LQQQQQQQQQQYLAAVMNQQR-SMGNERFQPMMY 540

BLAST of CmaCh06G014330 vs. ExPASy Swiss-Prot
Match: Q0WV37 (Heavy metal-associated isoprenylated plant protein 34 OS=Arabidopsis thaliana OX=3702 GN=HIPP34 PE=2 SV=1)

HSP 1 Score: 159.8 bits (403), Expect = 7.6e-38
Identity = 241/629 (38.31%), Postives = 288/629 (45.79%), Query Frame = 0

Query: 1   MSKEEFLKIQKCVLKVNIHCDGCKQKVKKILQKIDGVFTTEIDAEQGKVTVTGNVDAAVL 60
           M+K++ +K+Q CVLKVN+HC+GCK KVKK LQKI+GV++ + D EQG+VTVTGN+D A+L
Sbjct: 1   MNKQDVMKLQTCVLKVNVHCEGCKHKVKKQLQKIEGVYSVKADVEQGRVTVTGNIDPALL 60

Query: 61  IKKLSKSGKHAEIWGGQPANNNKNQQN--NIANQMKNMQI---DNAKGGNNKGQNQKGAA 120
           +KKLSKSGKHAEI GG         +   N+  Q  N+ +   +  KGG    Q +  A 
Sbjct: 61  VKKLSKSGKHAEILGGGGGGGGGGGKGFPNLNGQFANLNMGGNNKPKGGKESNQVKGKAG 120

Query: 121 ANGGNNQPKGGGQGQGLPPVQLQQLQQMK------GFQDLKLPPQFKGLKLPVKDQNANL 180
             GG  Q  G GQ   L P Q+QQ+  MK      G   +K+PP      +  KDQ    
Sbjct: 121 GGGGGGQNHGHGQPMQLNPQQIQQMMMMKAAHGGGGGGQMKMPP------MAAKDQK--- 180

Query: 181 PKGGKFNLHEGDDLSDENDEDDYDD----EDDYD----DDDELDDDL----------GNL 240
            K  KF     DD  DE  EDDYDD    EDDYD    DDDE DDD           G  
Sbjct: 181 -KSVKF----ADDEDDEFSEDDYDDEDFSEDDYDDDEFDDDEDDDDEMGHGHGHGGGGGN 240

Query: 241 HLPP-----ASKMKPMM----GNGQIPK------MMMNGNLPPVVNGPAGGGNAKKGGGA 300
           H+PP      +KM P M    GNG  PK      MMMNG          GGG  KKGGG 
Sbjct: 241 HMPPNKMMMPNKMMPQMGGHHGNGGGPKGPNEIMMMMNG-------FKGGGGGGKKGGGG 300

Query: 301 ---VPVQ---------------------GNNNGKNVTGGGGKG-----NGGGG------- 360
              +PVQ                     G   GK   GGG  G     +GGGG       
Sbjct: 301 GFEIPVQMKGMGEGKNGKDGKKGKGGEKGKKEGKENKGGGKTGKTDAKSGGGGLLGFFKK 360

Query: 361 ----------------GGGGSNQKQGGGGGSNQ-------KQGGGCGGG----------V 420
                           G GG+  K  GGGG  Q       K GGG  GG          +
Sbjct: 361 GKSGNGDEKKSAGKKDGHGGNKVKSHGGGGGVQHYDSGPKKGGGGTKGGGHGGLDIDELM 420

Query: 421 SNGKNGNGGG-----NGSSNGVNGGQKMTGGSNGMGHDLQSF--IGAHGLG--MSNLGQM 480
            + K G GGG     N S+ G+ GG    GG  G G  +     +G  G G  MS +G  
Sbjct: 421 KHSKGGGGGGNKGNHNHSAKGIGGGPMGPGGPMGPGGPMGQGGPMGMMGPGGPMSMMGPG 480

Query: 481 GDR-PM-GQMNNLPAVQGLPASAAAAMNGGGSAGRFIPDSISTNPYHHHHQQQQQQQQQH 487
           G   PM GQ  + PAVQGLP      M+GGG      P +            QQ  QQQ+
Sbjct: 481 GPMGPMGGQGGSYPAVQGLP------MSGGGGYYPGPPQA-----------SQQMNQQQY 540

BLAST of CmaCh06G014330 vs. ExPASy Swiss-Prot
Match: Q84J88 (Heavy metal-associated isoprenylated plant protein 36 OS=Arabidopsis thaliana OX=3702 GN=HIPP36 PE=1 SV=1)

HSP 1 Score: 99.4 bits (246), Expect = 1.2e-19
Identity = 65/153 (42.48%), Postives = 93/153 (60.78%), Query Frame = 0

Query: 5   EFLKIQKCVLKVNIHCDGCKQKVKKILQKIDGVFTTEIDAEQGKVTVTGNVDAAVLIKKL 64
           E L+    VL+V+IHC+GCK+K+KKIL KIDGV+TT ID +Q KVTV GNV+  +LIKK+
Sbjct: 25  EPLRYTTWVLRVSIHCEGCKRKIKKILSKIDGVYTTNIDVKQQKVTVIGNVEPEILIKKI 84

Query: 65  SKSGKHAEIWGGQPANNNKNQQNNIANQMKNMQIDNAKGGNNKGQNQKGAAANGGNNQPK 124
            K+G+HAE+W      + +N  NN  N  K  + DN +   ++  ++     NGG +   
Sbjct: 85  MKAGRHAELW----PTSMENNINNDCNYQKKPKKDNEETSGDEDDDENNNNNNGGGDVGG 144

Query: 125 GGGQGQGLPPVQLQQLQQMKGFQDLKLPPQFKG 158
           GGG G G       Q++Q+  F + +L PQ  G
Sbjct: 145 GGGGGGG----NFDQVKQVVTFVNGQLQPQGDG 169

BLAST of CmaCh06G014330 vs. ExPASy Swiss-Prot
Match: A2RVM8 (Heavy metal-associated isoprenylated plant protein 37 OS=Arabidopsis thaliana OX=3702 GN=HIPP37 PE=2 SV=1)

HSP 1 Score: 93.6 bits (231), Expect = 6.6e-18
Identity = 62/132 (46.97%), Postives = 85/132 (64.39%), Query Frame = 0

Query: 1   MSKEE---FLKIQKCVLKVNIHCDGCKQKVKKILQKIDGVFTTEIDAEQGKVTVTGNVDA 60
           M+K+E    LKIQ   L+VNIHC+GC +KVKK+LQ+I+GV   +I+AE  KVTV+G+VD+
Sbjct: 1   MTKDEDFKLLKIQTFSLRVNIHCEGCNKKVKKLLQRIEGVCHVKIEAEHQKVTVSGSVDS 60

Query: 61  AVLIKKLSKSGKHAEIWGGQPANNNKNQQNNIANQMKNMQIDNAKGGNNKGQNQKGAAAN 120
           A LI KL K+GKHAE+W   P N N+ Q+    + +KN+        N KGQ Q  A + 
Sbjct: 61  ATLINKLVKAGKHAELWSPNP-NQNQPQKPKTNDFIKNV--------NQKGQKQGSAKSG 120

Query: 121 GGNNQPKGGGQG 130
               +PK G +G
Sbjct: 121 IEACKPKNGPKG 123

BLAST of CmaCh06G014330 vs. ExPASy TrEMBL
Match: A0A6J1IC55 (heavy metal-associated isoprenylated plant protein 32 OS=Cucurbita maxima OX=3661 GN=LOC111472321 PE=4 SV=1)

HSP 1 Score: 891.7 bits (2303), Expect = 1.3e-255
Identity = 486/486 (100.00%), Postives = 486/486 (100.00%), Query Frame = 0

Query: 1   MSKEEFLKIQKCVLKVNIHCDGCKQKVKKILQKIDGVFTTEIDAEQGKVTVTGNVDAAVL 60
           MSKEEFLKIQKCVLKVNIHCDGCKQKVKKILQKIDGVFTTEIDAEQGKVTVTGNVDAAVL
Sbjct: 1   MSKEEFLKIQKCVLKVNIHCDGCKQKVKKILQKIDGVFTTEIDAEQGKVTVTGNVDAAVL 60

Query: 61  IKKLSKSGKHAEIWGGQPANNNKNQQNNIANQMKNMQIDNAKGGNNKGQNQKGAAANGGN 120
           IKKLSKSGKHAEIWGGQPANNNKNQQNNIANQMKNMQIDNAKGGNNKGQNQKGAAANGGN
Sbjct: 61  IKKLSKSGKHAEIWGGQPANNNKNQQNNIANQMKNMQIDNAKGGNNKGQNQKGAAANGGN 120

Query: 121 NQPKGGGQGQGLPPVQLQQLQQMKGFQDLKLPPQFKGLKLPVKDQNANLPKGGKFNLHEG 180
           NQPKGGGQGQGLPPVQLQQLQQMKGFQDLKLPPQFKGLKLPVKDQNANLPKGGKFNLHEG
Sbjct: 121 NQPKGGGQGQGLPPVQLQQLQQMKGFQDLKLPPQFKGLKLPVKDQNANLPKGGKFNLHEG 180

Query: 181 DDLSDENDEDDYDDEDDYDDDDELDDDLGNLHLPPASKMKPMMGNGQIPKMMMNGNLPPV 240
           DDLSDENDEDDYDDEDDYDDDDELDDDLGNLHLPPASKMKPMMGNGQIPKMMMNGNLPPV
Sbjct: 181 DDLSDENDEDDYDDEDDYDDDDELDDDLGNLHLPPASKMKPMMGNGQIPKMMMNGNLPPV 240

Query: 241 VNGPAGGGNAKKGGGAVPVQGNNNGKNVTGGGGKGNGGGGGGGGSNQKQGGGGGSNQKQG 300
           VNGPAGGGNAKKGGGAVPVQGNNNGKNVTGGGGKGNGGGGGGGGSNQKQGGGGGSNQKQG
Sbjct: 241 VNGPAGGGNAKKGGGAVPVQGNNNGKNVTGGGGKGNGGGGGGGGSNQKQGGGGGSNQKQG 300

Query: 301 GGCGGGVSNGKNGNGGGNGSSNGVNGGQKMTGGSNGMGHDLQSFIGAHGLGMSNLGQMGD 360
           GGCGGGVSNGKNGNGGGNGSSNGVNGGQKMTGGSNGMGHDLQSFIGAHGLGMSNLGQMGD
Sbjct: 301 GGCGGGVSNGKNGNGGGNGSSNGVNGGQKMTGGSNGMGHDLQSFIGAHGLGMSNLGQMGD 360

Query: 361 RPMGQMNNLPAVQGLPASAAAAMNGGGSAGRFIPDSISTNPYHHHHQQQQQQQQQHMAQM 420
           RPMGQMNNLPAVQGLPASAAAAMNGGGSAGRFIPDSISTNPYHHHHQQQQQQQQQHMAQM
Sbjct: 361 RPMGQMNNLPAVQGLPASAAAAMNGGGSAGRFIPDSISTNPYHHHHQQQQQQQQQHMAQM 420

Query: 421 MNQQQHALGHQPMMYARPPPAVNYVPPPYPYPYPYPPPYHHPYPPPQQPEPYTYFSDENP 480
           MNQQQHALGHQPMMYARPPPAVNYVPPPYPYPYPYPPPYHHPYPPPQQPEPYTYFSDENP
Sbjct: 421 MNQQQHALGHQPMMYARPPPAVNYVPPPYPYPYPYPPPYHHPYPPPQQPEPYTYFSDENP 480

Query: 481 SSCNIM 487
           SSCNIM
Sbjct: 481 SSCNIM 486

BLAST of CmaCh06G014330 vs. ExPASy TrEMBL
Match: A0A6J1FEU1 (heavy metal-associated isoprenylated plant protein 32 OS=Cucurbita moschata OX=3662 GN=LOC111443301 PE=4 SV=1)

HSP 1 Score: 817.0 bits (2109), Expect = 4.2e-233
Identity = 464/490 (94.69%), Postives = 473/490 (96.53%), Query Frame = 0

Query: 1   MSKEEFLKIQKCVLKVNIHCDGCKQKVKKILQKIDGVFTTEIDAEQGKVTVTGNVDAAVL 60
           MSKEEFLKIQKCVLKVNIHCDGCK KVKKILQKIDGVFTTEIDAEQGKVTVTGNVDAAVL
Sbjct: 1   MSKEEFLKIQKCVLKVNIHCDGCKHKVKKILQKIDGVFTTEIDAEQGKVTVTGNVDAAVL 60

Query: 61  IKKLSKSGKHAEIWGGQPANNNKNQQNNIANQMKNMQIDNAKGGNNKGQNQKGAAANGGN 120
           +KKL+KSGKHAEIWGGQPANNNKNQQNNIANQMKNMQIDNAKGGNNKGQNQKGAAANGGN
Sbjct: 61  MKKLAKSGKHAEIWGGQPANNNKNQQNNIANQMKNMQIDNAKGGNNKGQNQKGAAANGGN 120

Query: 121 NQPKGGGQGQGLPP--VQLQQLQQMKGFQDLKLPPQFKGLKLPVKDQNANLPKGGKFNLH 180
           NQPKGGGQGQGLPP  VQLQQLQQMKGFQDLKLPPQFKGLKLPVKDQN NLPKGGKFNLH
Sbjct: 121 NQPKGGGQGQGLPPVQVQLQQLQQMKGFQDLKLPPQFKGLKLPVKDQNVNLPKGGKFNLH 180

Query: 181 EGDDLSDENDEDDYDDEDDYDDDDELDDDLGNLHLPPASKMKPMMGNGQIPKMMMNGNLP 240
           EGDDLSDE DED+Y+DEDDYDD+DELDDDLGNLHLPPASKMKPMMGNGQIPKMMMNGNLP
Sbjct: 181 EGDDLSDE-DEDEYEDEDDYDDEDELDDDLGNLHLPPASKMKPMMGNGQIPKMMMNGNLP 240

Query: 241 PVVNGPAGGGNAKKGGGAVPVQGNNNGKNVTGGGGKGNGGGGGGGGSNQKQGGGGGSNQK 300
           PVVNGPAGGGNAKKGGGAV VQGNNNGKN  GGG KGNGGGGGGGGSNQKQGGGGGSNQK
Sbjct: 241 PVVNGPAGGGNAKKGGGAVSVQGNNNGKNGNGGGSKGNGGGGGGGGSNQKQGGGGGSNQK 300

Query: 301 QGGGCGGGVSNGKNGNGGGNGSSNGVNGGQKMTGGSNGMGHDLQSFIGAHGLGMSNLGQM 360
           QGGG GGG SNGKNGNGGGNGSSNGVNGGQK+TGG+NGMGHDLQSFIGAHGLGMSNLGQM
Sbjct: 301 QGGG-GGGGSNGKNGNGGGNGSSNGVNGGQKVTGGANGMGHDLQSFIGAHGLGMSNLGQM 360

Query: 361 GDRPMGQMNNLPAVQGLPASAAAAMNGGGSAGRFIPDSISTNPYHHHHQQQQQQQQQHMA 420
           GDRPMGQMNNLPAVQGLPASAAAAMNGGGSAGRF+PDSISTNPY    QQQQQQQQQ+MA
Sbjct: 361 GDRPMGQMNNLPAVQGLPASAAAAMNGGGSAGRFMPDSISTNPY--QQQQQQQQQQQYMA 420

Query: 421 QMMNQQQHALGHQPMMYARPPPAVNYVPPPYPYPYPYPPPY-HHPYPPP-QQPEPYTYFS 480
           QMMNQQQHALGHQPMMYARPPPAVNYVPPPYPYPYPYPPPY HHPYPPP QQPEPYTYFS
Sbjct: 421 QMMNQQQHALGHQPMMYARPPPAVNYVPPPYPYPYPYPPPYHHHPYPPPQQQPEPYTYFS 480

Query: 481 DENPSSCNIM 487
           DENPSSCNIM
Sbjct: 481 DENPSSCNIM 486

BLAST of CmaCh06G014330 vs. ExPASy TrEMBL
Match: A0A6J1ITG7 (heavy metal-associated isoprenylated plant protein 32-like OS=Cucurbita maxima OX=3661 GN=LOC111479190 PE=4 SV=1)

HSP 1 Score: 642.9 bits (1657), Expect = 1.1e-180
Identity = 399/507 (78.70%), Postives = 416/507 (82.05%), Query Frame = 0

Query: 1   MSKEEFLKIQKCVLKVNIHCDGCKQKVKKILQKIDGVFTTEIDAEQGKVTVTGNVDAAVL 60
           MSKEEFLKIQKCVLKVNIHCDGCK KVKKILQKIDGVFTTEIDAEQGKVTVTGNVDA VL
Sbjct: 1   MSKEEFLKIQKCVLKVNIHCDGCKHKVKKILQKIDGVFTTEIDAEQGKVTVTGNVDAVVL 60

Query: 61  IKKLSKSGKHAEIWGGQPANNNKNQQNNIANQMKNMQIDNAKGGNNKGQNQKGAAANG-- 120
           IKKL KSGKHAE+WGGQP  NNKNQQNNIA+QMKNMQIDNAKGGNNKGQNQKG  A G  
Sbjct: 61  IKKLLKSGKHAEVWGGQP-GNNKNQQNNIASQMKNMQIDNAKGGNNKGQNQKGGGAGGGG 120

Query: 121 --GNNQPKGGGQGQGLPPVQLQQLQQMKGFQDLKLPPQFKGLKLPVKDQNANLPKGGKFN 180
             GNN PK  GQGQGL P QLQQLQ MKGFQDLKLPPQF GLKLPVKDQN N  K GKFN
Sbjct: 121 GNGNNLPK-VGQGQGLQPAQLQQLQAMKGFQDLKLPPQFNGLKLPVKDQNPNQAKAGKFN 180

Query: 181 LHEGDDLSDENDEDDYDDEDDYDDDDELDDDLGNLHLPPASKMKPMMGNGQIPKMMMNGN 240
           L EGDDLSDE D+ DYDDEDDYDDDDE DDDLGNLHLPPA+KMKPMMGNGQIPKMMMNGN
Sbjct: 181 LQEGDDLSDE-DDYDYDDEDDYDDDDEFDDDLGNLHLPPANKMKPMMGNGQIPKMMMNGN 240

Query: 241 LPPVVNGPA----GGGNAKKGGGAV-PVQGNNNGKNVTGGGGKGNGGGGGGGGSNQKQGG 300
            P V+NGPA    GGGN  KGGGAV PVQGNNN KN  GGGGKGN GGGG GGSNQKQGG
Sbjct: 241 QPAVMNGPAVGGNGGGNGNKGGGAVPPVQGNNNAKNGNGGGGKGN-GGGGSGGSNQKQGG 300

Query: 301 GGGSNQKQGGGCGGGVSNGKNGNGGGNGSSNGVNGGQKMTGGSNGMGHDLQSFIGAHGLG 360
           GGG N   GGG GGGV+N K G+G GNGSSNG+N GQK  GG+NGMGHDLQS +GAHGLG
Sbjct: 301 GGGKNNGGGGGGGGGVTN-KTGHGVGNGSSNGMNSGQKAAGGANGMGHDLQSLVGAHGLG 360

Query: 361 MSNL----------GQMGDRPMGQMNNLPAVQGLPASA-AAAMNGGGSAGRFIPDSISTN 420
           MSNL          GQMGDRPMGQM N+PAVQGLPA+A A  MNGGG  GRF P  ++ +
Sbjct: 361 MSNLSQMGNMGVQMGQMGDRPMGQMGNIPAVQGLPAAAPAPTMNGGGGGGRFSPQDMAGH 420

Query: 421 PYHHHHQQQQQQQQQHMAQMMNQQQ-HALGHQPMMYARPPPAVNYVPPPYPYPYPYPPPY 480
           PY        QQ  Q+MAQMMNQQQ HALGHQPMMYARPPPAVNYVPPPYPYP PY    
Sbjct: 421 PY--------QQNPQYMAQMMNQQQHHALGHQPMMYARPPPAVNYVPPPYPYPPPY---- 480

Query: 481 HHPYPPPQQPEPYTYFSDENPSSCNIM 487
            HPYPPP QPEPYTYFSDENPSSC+IM
Sbjct: 481 -HPYPPP-QPEPYTYFSDENPSSCHIM 488

BLAST of CmaCh06G014330 vs. ExPASy TrEMBL
Match: A0A6J1GW78 (heavy metal-associated isoprenylated plant protein 32-like OS=Cucurbita moschata OX=3662 GN=LOC111457714 PE=4 SV=1)

HSP 1 Score: 642.5 bits (1656), Expect = 1.4e-180
Identity = 397/509 (78.00%), Postives = 418/509 (82.12%), Query Frame = 0

Query: 1   MSKEEFLKIQKCVLKVNIHCDGCKQKVKKILQKIDGVFTTEIDAEQGKVTVTGNVDAAVL 60
           MSKEEFLKIQKCVLKVNIHCDGCK KVKKILQKIDGVFTTEIDAEQGKVTVTGNVDA+VL
Sbjct: 1   MSKEEFLKIQKCVLKVNIHCDGCKHKVKKILQKIDGVFTTEIDAEQGKVTVTGNVDASVL 60

Query: 61  IKKLSKSGKHAEIWGGQPANNNKNQQNNIANQMKNMQIDNAKGGNNKGQNQKGAAANG-- 120
           IKKL KSGKHAE+WGGQP  NNKNQQNNIA+QMKNMQIDNAKGGN KGQNQKG    G  
Sbjct: 61  IKKLLKSGKHAEVWGGQP-GNNKNQQNNIASQMKNMQIDNAKGGNIKGQNQKGGGGAGGG 120

Query: 121 ----GNNQPKGG-GQGQGLPPVQLQQLQQMKGFQDLKLPPQFKGLKLPVKDQNANLPKGG 180
               GNN PKGG GQ QGL P QLQQLQ MKGFQDLKLPPQF GLKLPVKDQN N  K G
Sbjct: 121 GGGNGNNLPKGGQGQVQGLQPAQLQQLQAMKGFQDLKLPPQFNGLKLPVKDQNPNQAKAG 180

Query: 181 KFNLHEGDDLSDENDEDDYDDEDDYDDDDELDDDLGNLHLPPASKMKPMMGNGQIPKMMM 240
           KFNL EGDDLSDE+D DDYDDEDDYDDDDE DDDLGNLHLPPA+KMKPMMGNGQIPKMMM
Sbjct: 181 KFNLQEGDDLSDEDDYDDYDDEDDYDDDDEFDDDLGNLHLPPANKMKPMMGNGQIPKMMM 240

Query: 241 NGNLPPVVNGPA----GGGNAKKGGGAVPVQGNNNGKNVTGGGGKGNGGGGGGGGSNQKQ 300
           NGN P V+NGPA    GGGN KKGGGAVPVQGNNN KN  GGGGKGN GGGG GGSNQKQ
Sbjct: 241 NGNHPAVMNGPAVGGNGGGNGKKGGGAVPVQGNNNAKNGNGGGGKGN-GGGGSGGSNQKQ 300

Query: 301 GGGGGSNQKQGGGCGGGVSNGKNGNGGGNGSSNGVNGGQKMTGGSNGMGHDLQSFIGAHG 360
           GGGGG N   GGG GGGV+N + G+GGGNGSSNG+N GQK  GG+NGMGHDLQS +GAHG
Sbjct: 301 GGGGGKN--NGGGGGGGVTN-QTGHGGGNGSSNGMNSGQKAAGGANGMGHDLQSLVGAHG 360

Query: 361 LGMSNL----------GQMGDRPMGQMNNLPAVQGLPASA-AAAMNGGGSAGRFIPDSIS 420
           LGMSNL          GQMGDRPMGQ+ N+PAVQGLPA+A A  MNGG   GRF P  ++
Sbjct: 361 LGMSNLSQMGNMGVQMGQMGDRPMGQIGNIPAVQGLPAAAPAPTMNGGSGGGRFSPLDMA 420

Query: 421 TNPYHHHHQQQQQQQQQHMAQMMNQQQ-HALGHQPMMYARPPPAVNYVPPPYPYPYPYPP 480
            +PY        QQ  Q++AQMMNQQQ HALGHQPMMYARPPPAVNYVPPPYPYP PY  
Sbjct: 421 GHPY--------QQNPQYIAQMMNQQQHHALGHQPMMYARPPPAVNYVPPPYPYPPPY-- 480

Query: 481 PYHHPYPPPQQPEPYTYFSDENPSSCNIM 487
              HPYPPP QPEPYTYFSDENPSSC+IM
Sbjct: 481 ---HPYPPP-QPEPYTYFSDENPSSCHIM 490

BLAST of CmaCh06G014330 vs. ExPASy TrEMBL
Match: A0A5D3D1Q8 (WAG22 antigen OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold434G00080 PE=4 SV=1)

HSP 1 Score: 642.1 bits (1655), Expect = 1.9e-180
Identity = 400/515 (77.67%), Postives = 419/515 (81.36%), Query Frame = 0

Query: 1   MSKEEFLKIQKCVLKVNIHCDGCKQKVKKILQKIDGVFTTEIDAEQGKVTVTGNVDAAVL 60
           MSKEEFLKIQKCVLKVNIHCDGCK KVKKILQKIDGVFTTEIDAEQGKVTVTGNVDAAVL
Sbjct: 1   MSKEEFLKIQKCVLKVNIHCDGCKHKVKKILQKIDGVFTTEIDAEQGKVTVTGNVDAAVL 60

Query: 61  IKKLSKSGKHAEIWGGQPANNNKNQQNNIANQMKNMQIDNAKGGNNKGQNQKGAAANG-- 120
           IKKL+KSGKHAEIWG QPANNNKNQQNNIANQMKNMQIDNAKGGNNKGQNQKG AA G  
Sbjct: 61  IKKLAKSGKHAEIWGSQPANNNKNQQNNIANQMKNMQIDNAKGGNNKGQNQKGGAAGGGG 120

Query: 121 ----GNNQPKGGGQGQGLPPVQLQQLQQMKGFQDLKLPPQFKGLKLPVKDQNANLPKGGK 180
               GNN PK GG  QGL P QLQQLQ MKGFQDLKLPPQFKGLKLPVKDQN NL K GK
Sbjct: 121 GGGNGNNLPK-GGHVQGLQPSQLQQLQAMKGFQDLKLPPQFKGLKLPVKDQNPNLSKAGK 180

Query: 181 FNLHEGDDLSDENDEDDYDDEDDYDDDDELDDDLGNLHLPPASKMKPMMGNGQIPKMMMN 240
           FNLHE DDLSDE+D DDYDDE DYDDDDE DDDLGNLHLPPA+KMKP+MGN QIPKMM+N
Sbjct: 181 FNLHEDDDLSDEDDLDDYDDE-DYDDDDEFDDDLGNLHLPPANKMKPIMGNAQIPKMMVN 240

Query: 241 GNLPPVVNGPA----GGGNAKKGGGAVPVQGNNNGKNVTGGGGKGN-GGGGGGGGSNQKQ 300
           GN P V+N P     GGGN KKGGGAVPVQ NNN KN   GGGKGN GGGGGGGGSNQKQ
Sbjct: 241 GNHPAVMNSPGAGGNGGGNGKKGGGAVPVQVNNNAKNGNNGGGKGNGGGGGGGGGSNQKQ 300

Query: 301 GGGGGSNQKQGGGCGGG-------VSNGKNGNGGGNGSSNGVNGGQKMTGGSNGMGHDLQ 360
           G GGG  +  GGG GGG         N K G+GGGNGS+NG+NGGQK  GG+NGMG DLQ
Sbjct: 301 GSGGGGGKNNGGGGGGGGGGHGGAGGNNKTGHGGGNGSNNGMNGGQKAAGGANGMGQDLQ 360

Query: 361 SFIGAHGLGMSN------LGQMGDRPMGQMNNLPAVQGLPAS-AAAAMNG---GGSAGRF 420
           S +GAHGLGMSN      LGQMGDR MGQM NL AVQGLPA+  A  MNG   GG A RF
Sbjct: 361 SLVGAHGLGMSNLSQMGQLGQMGDRTMGQMGNLSAVQGLPAAPPAPTMNGNGNGGGASRF 420

Query: 421 IPDSISTNPYHHHHQQQQQQQQQHMAQMMNQQQ-HALGHQPMMYARPPPAVNYVPPPYPY 480
           + + ++ NPY       QQ  QQ+MAQMMNQQQ HALGHQPMMYARPPPAVNYVPP  PY
Sbjct: 421 LAEGMTGNPY-------QQHPQQYMAQMMNQQQHHALGHQPMMYARPPPAVNYVPP--PY 480

Query: 481 PYPYPPPYHHPYPPPQQPEPYTYFSDENPSSCNIM 487
           PYPYPPPYH+PY PP QPEPYTYFSDENPSSCNIM
Sbjct: 481 PYPYPPPYHNPY-PPTQPEPYTYFSDENPSSCNIM 503

BLAST of CmaCh06G014330 vs. NCBI nr
Match: XP_022973750.1 (heavy metal-associated isoprenylated plant protein 32 [Cucurbita maxima])

HSP 1 Score: 891.7 bits (2303), Expect = 2.8e-255
Identity = 486/486 (100.00%), Postives = 486/486 (100.00%), Query Frame = 0

Query: 1   MSKEEFLKIQKCVLKVNIHCDGCKQKVKKILQKIDGVFTTEIDAEQGKVTVTGNVDAAVL 60
           MSKEEFLKIQKCVLKVNIHCDGCKQKVKKILQKIDGVFTTEIDAEQGKVTVTGNVDAAVL
Sbjct: 1   MSKEEFLKIQKCVLKVNIHCDGCKQKVKKILQKIDGVFTTEIDAEQGKVTVTGNVDAAVL 60

Query: 61  IKKLSKSGKHAEIWGGQPANNNKNQQNNIANQMKNMQIDNAKGGNNKGQNQKGAAANGGN 120
           IKKLSKSGKHAEIWGGQPANNNKNQQNNIANQMKNMQIDNAKGGNNKGQNQKGAAANGGN
Sbjct: 61  IKKLSKSGKHAEIWGGQPANNNKNQQNNIANQMKNMQIDNAKGGNNKGQNQKGAAANGGN 120

Query: 121 NQPKGGGQGQGLPPVQLQQLQQMKGFQDLKLPPQFKGLKLPVKDQNANLPKGGKFNLHEG 180
           NQPKGGGQGQGLPPVQLQQLQQMKGFQDLKLPPQFKGLKLPVKDQNANLPKGGKFNLHEG
Sbjct: 121 NQPKGGGQGQGLPPVQLQQLQQMKGFQDLKLPPQFKGLKLPVKDQNANLPKGGKFNLHEG 180

Query: 181 DDLSDENDEDDYDDEDDYDDDDELDDDLGNLHLPPASKMKPMMGNGQIPKMMMNGNLPPV 240
           DDLSDENDEDDYDDEDDYDDDDELDDDLGNLHLPPASKMKPMMGNGQIPKMMMNGNLPPV
Sbjct: 181 DDLSDENDEDDYDDEDDYDDDDELDDDLGNLHLPPASKMKPMMGNGQIPKMMMNGNLPPV 240

Query: 241 VNGPAGGGNAKKGGGAVPVQGNNNGKNVTGGGGKGNGGGGGGGGSNQKQGGGGGSNQKQG 300
           VNGPAGGGNAKKGGGAVPVQGNNNGKNVTGGGGKGNGGGGGGGGSNQKQGGGGGSNQKQG
Sbjct: 241 VNGPAGGGNAKKGGGAVPVQGNNNGKNVTGGGGKGNGGGGGGGGSNQKQGGGGGSNQKQG 300

Query: 301 GGCGGGVSNGKNGNGGGNGSSNGVNGGQKMTGGSNGMGHDLQSFIGAHGLGMSNLGQMGD 360
           GGCGGGVSNGKNGNGGGNGSSNGVNGGQKMTGGSNGMGHDLQSFIGAHGLGMSNLGQMGD
Sbjct: 301 GGCGGGVSNGKNGNGGGNGSSNGVNGGQKMTGGSNGMGHDLQSFIGAHGLGMSNLGQMGD 360

Query: 361 RPMGQMNNLPAVQGLPASAAAAMNGGGSAGRFIPDSISTNPYHHHHQQQQQQQQQHMAQM 420
           RPMGQMNNLPAVQGLPASAAAAMNGGGSAGRFIPDSISTNPYHHHHQQQQQQQQQHMAQM
Sbjct: 361 RPMGQMNNLPAVQGLPASAAAAMNGGGSAGRFIPDSISTNPYHHHHQQQQQQQQQHMAQM 420

Query: 421 MNQQQHALGHQPMMYARPPPAVNYVPPPYPYPYPYPPPYHHPYPPPQQPEPYTYFSDENP 480
           MNQQQHALGHQPMMYARPPPAVNYVPPPYPYPYPYPPPYHHPYPPPQQPEPYTYFSDENP
Sbjct: 421 MNQQQHALGHQPMMYARPPPAVNYVPPPYPYPYPYPPPYHHPYPPPQQPEPYTYFSDENP 480

Query: 481 SSCNIM 487
           SSCNIM
Sbjct: 481 SSCNIM 486

BLAST of CmaCh06G014330 vs. NCBI nr
Match: XP_023522019.1 (heavy metal-associated isoprenylated plant protein 32-like [Cucurbita pepo subsp. pepo] >XP_023538837.1 heavy metal-associated isoprenylated plant protein 32-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 827.8 bits (2137), Expect = 4.9e-236
Identity = 467/489 (95.50%), Postives = 474/489 (96.93%), Query Frame = 0

Query: 1   MSKEEFLKIQKCVLKVNIHCDGCKQKVKKILQKIDGVFTTEIDAEQGKVTVTGNVDAAVL 60
           MSKEEFLKIQKCVLKVNIHCDGCK KVKKILQKIDGVFTTEIDAEQGKVTVTGNVDAAVL
Sbjct: 1   MSKEEFLKIQKCVLKVNIHCDGCKHKVKKILQKIDGVFTTEIDAEQGKVTVTGNVDAAVL 60

Query: 61  IKKLSKSGKHAEIWGGQPANNNKNQQNNIANQMKNMQIDNAKGGNNKGQNQKGAAANGGN 120
           IKKL+KSGKHAEIWGGQPANNNKNQQNNIANQMKNMQIDNAKGGNNKGQNQKGAAANGGN
Sbjct: 61  IKKLAKSGKHAEIWGGQPANNNKNQQNNIANQMKNMQIDNAKGGNNKGQNQKGAAANGGN 120

Query: 121 NQPKGGGQGQGLPP--VQLQQLQQMKGFQDLKLPPQFKGLKLPVKDQNANLPKGGKFNLH 180
           NQPKGGGQGQGLPP  VQLQQLQQMKGFQDLKLPPQFKGLKLPVKDQN NLPKGGKFNLH
Sbjct: 121 NQPKGGGQGQGLPPVQVQLQQLQQMKGFQDLKLPPQFKGLKLPVKDQNVNLPKGGKFNLH 180

Query: 181 EGDDLSDENDEDDYDDEDDYDDDDELDDDLGNLHLPPASKMKPMMGNGQIPKMMMNGNLP 240
           EGDDLSDE DED+YDDEDDYDD+DELDDDLGNLHLPPASKMKPMMGNGQIPK+MMNGNLP
Sbjct: 181 EGDDLSDE-DEDEYDDEDDYDDEDELDDDLGNLHLPPASKMKPMMGNGQIPKIMMNGNLP 240

Query: 241 PVVNGPAGGGNAKKGGGAVPVQGNNNGKNVTGGGGKGNGGGGGGGGSNQKQGGGGGSNQK 300
           PVVNGPAGGGNAKKGGGAVPVQGNNNGKN  GGGGKGNGGGGGGGGSNQKQGGGGGSNQK
Sbjct: 241 PVVNGPAGGGNAKKGGGAVPVQGNNNGKNGNGGGGKGNGGGGGGGGSNQKQGGGGGSNQK 300

Query: 301 QGGGCGGGVSNGKNGNGGGNGSSNGVNGGQKMTGGSNGMGHDLQSFIGAHGLGMSNLGQM 360
           QGGG GGG SNGKNGNGGGNGSSNGVNGGQKMTGG+NGMGHDLQSFIGAHGLGMSNLGQM
Sbjct: 301 QGGG-GGGGSNGKNGNGGGNGSSNGVNGGQKMTGGANGMGHDLQSFIGAHGLGMSNLGQM 360

Query: 361 GDRPMGQMNNLPAVQGLPASAAAAMNGGGSAGRFIPDSISTNPYHHHHQQQQQQQQQHMA 420
           GDRPMGQMNNLPAVQGLPASAAAAMNGGGSAGRF+PDSIS NPY    QQQQQQQQQ+MA
Sbjct: 361 GDRPMGQMNNLPAVQGLPASAAAAMNGGGSAGRFMPDSISANPY-QQQQQQQQQQQQYMA 420

Query: 421 QMMNQQQHALGHQPMMYARPPPAVNYVPPPYPYPYPYPPPYHHPYPPP-QQPEPYTYFSD 480
           QMMNQQQHALGHQPMMYARPPPAVNYVPPPYPYPYPYPPPYHHPYPPP QQPEPYTYFSD
Sbjct: 421 QMMNQQQHALGHQPMMYARPPPAVNYVPPPYPYPYPYPPPYHHPYPPPQQQPEPYTYFSD 480

Query: 481 ENPSSCNIM 487
           ENPSSCNIM
Sbjct: 481 ENPSSCNIM 486

BLAST of CmaCh06G014330 vs. NCBI nr
Match: KAG7028924.1 (Heavy metal-associated isoprenylated plant protein 32 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 820.1 bits (2117), Expect = 1.0e-233
Identity = 465/492 (94.51%), Postives = 475/492 (96.54%), Query Frame = 0

Query: 1   MSKEEFLKIQKCVLKVNIHCDGCKQKVKKILQKIDGVFTTEIDAEQGKVTVTGNVDAAVL 60
           MSKEEFLKIQKCVLKVNIHCDGCK KVKKILQKIDGVFTTEIDAEQGKVTVTGNVDAAVL
Sbjct: 1   MSKEEFLKIQKCVLKVNIHCDGCKHKVKKILQKIDGVFTTEIDAEQGKVTVTGNVDAAVL 60

Query: 61  IKKLSKSGKHAEIWGGQPANNNKNQQNNIANQMKNMQIDNAKGGNNKGQNQKGAAANGGN 120
           +KKL+KSGKHAEIWGGQPANNNKNQQNNIANQMKNMQIDNAKGGNNKGQNQKGAAANGGN
Sbjct: 61  MKKLAKSGKHAEIWGGQPANNNKNQQNNIANQMKNMQIDNAKGGNNKGQNQKGAAANGGN 120

Query: 121 NQPKGGGQGQGLPP--VQLQQLQQMKGFQDLKLPPQFKGLKLPVKDQNANLPKGGKFNLH 180
           NQPKGGGQGQGLPP  VQLQQLQQMKGFQDLKLPPQFKGLKLPVKDQN NLPKGGKFNLH
Sbjct: 121 NQPKGGGQGQGLPPVQVQLQQLQQMKGFQDLKLPPQFKGLKLPVKDQNVNLPKGGKFNLH 180

Query: 181 EGDDLSDENDEDDYDDEDDYDDDDELDDDLGNLHLPPASKMKPMMGNGQIPKMMMNGNLP 240
           EGDDLSDE DED+Y+DE+DYDD+DELDDDLGNLHLPPASKMKPMMGNGQIPKMMMNGNLP
Sbjct: 181 EGDDLSDE-DEDEYEDEEDYDDEDELDDDLGNLHLPPASKMKPMMGNGQIPKMMMNGNLP 240

Query: 241 PVVNGPAGGGNAKKGGGAVPVQGNNNGKNVTGGGGKGNGGGGGGGGSNQKQGGGGGSNQK 300
           PVVNGPAGGGNAKKGGGAVPVQGNNNGKN  GGGGKGNGGGGGGGGSNQKQGGGGGSNQK
Sbjct: 241 PVVNGPAGGGNAKKGGGAVPVQGNNNGKNGNGGGGKGNGGGGGGGGSNQKQGGGGGSNQK 300

Query: 301 QGGGCGGGVSNGKNGNGGGNGSSNGVNGGQKMTGGSNGMGHDLQSFIGAHGLGMSNLGQM 360
           QGGG GGG SNGKNGNGGGNGSSNGVNGGQK+TGG+NGMGHDLQSFIGAHGLGMSNLGQM
Sbjct: 301 QGGG-GGGGSNGKNGNGGGNGSSNGVNGGQKVTGGANGMGHDLQSFIGAHGLGMSNLGQM 360

Query: 361 GDRPMGQMNNLPAVQGLPASAAAAMNGGGSAGRFIPDSISTNPY--HHHHQQQQQQQQQH 420
           GDRPMGQMNNLPAVQGLPASAAAAMNGGGSAGRF+PDSISTNPY      QQQQQQQQQ+
Sbjct: 361 GDRPMGQMNNLPAVQGLPASAAAAMNGGGSAGRFMPDSISTNPYQQQQQQQQQQQQQQQY 420

Query: 421 MAQMMNQQQHALGHQPMMYARPPPAVNYVPPPYPYPYPYPPPY-HHPYPPP-QQPEPYTY 480
           MAQMMNQQQHALGHQPMMYARPPPAVNYVPPPYPYPYPYPPPY HHPYPPP QQPEPYTY
Sbjct: 421 MAQMMNQQQHALGHQPMMYARPPPAVNYVPPPYPYPYPYPPPYHHHPYPPPQQQPEPYTY 480

Query: 481 FSDENPSSCNIM 487
           FSDENPSSCNIM
Sbjct: 481 FSDENPSSCNIM 490

BLAST of CmaCh06G014330 vs. NCBI nr
Match: XP_023522188.1 (heavy metal-associated isoprenylated plant protein 32-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 818.9 bits (2114), Expect = 2.3e-233
Identity = 465/490 (94.90%), Postives = 471/490 (96.12%), Query Frame = 0

Query: 1   MSKEEFLKIQKCVLKVNIHCDGCKQKVKKILQKIDGVFTTEIDAEQGKVTVTGNVDAAVL 60
           MSKEEFLKIQKCVLKVNIHCDGCK KVKKILQKIDGVFTTEIDAEQGKVTVTGNVDAAVL
Sbjct: 1   MSKEEFLKIQKCVLKVNIHCDGCKHKVKKILQKIDGVFTTEIDAEQGKVTVTGNVDAAVL 60

Query: 61  IKKLSKSGKHAEIWGGQPANNNKNQQNNIANQMKNMQIDNAKGGNNKGQNQKGAAANGGN 120
           IKKL+KSGKHAEIWGGQPANNNKNQQNNIANQMKNMQIDNAKGGNNKGQNQKGAAANGGN
Sbjct: 61  IKKLAKSGKHAEIWGGQPANNNKNQQNNIANQMKNMQIDNAKGGNNKGQNQKGAAANGGN 120

Query: 121 NQPKGGGQGQGLPP--VQLQQLQQMKGFQDLKLPPQFKGLKLPVKDQNANLPKGGKFNLH 180
           NQPKGGGQGQGLPP  VQLQQLQQMKGFQDLKLPPQFKGLKLPVKDQN NLPKGGKFNLH
Sbjct: 121 NQPKGGGQGQGLPPVQVQLQQLQQMKGFQDLKLPPQFKGLKLPVKDQNVNLPKGGKFNLH 180

Query: 181 EGDDLSDENDEDDYDDEDDYDDDDELDDDLGNLHLPPASKMKPMMGNGQIPKMMMNGNLP 240
           EGDDLSDE DED+YDDEDDYDD+DELDDDLG LHLPPASKMKPMMGNGQIPKMMMNG LP
Sbjct: 181 EGDDLSDE-DEDEYDDEDDYDDEDELDDDLGKLHLPPASKMKPMMGNGQIPKMMMNGKLP 240

Query: 241 PVVNGPAGGGNAKKGGGAVPVQGNNNGKNVTGGGGKGNGGGGGGGGSNQKQGGGGGSNQK 300
           PVVNGPAGGGNAKKGGGAVPVQGNNNGKN  GGGGKGNGGGGGGGGSNQKQGGGGGSNQK
Sbjct: 241 PVVNGPAGGGNAKKGGGAVPVQGNNNGKNGNGGGGKGNGGGGGGGGSNQKQGGGGGSNQK 300

Query: 301 QGGGCGGGVSNGKNGNGGGNGSSNGVNGGQKMTGGSNGMGHDLQSFIGAHGLGMSNLGQM 360
           QGG  GGG SNGKNGNGGGNGSSNGVNGGQKMTGG+NGMGHDLQSFIGAHGLGMSNLGQM
Sbjct: 301 QGG--GGGGSNGKNGNGGGNGSSNGVNGGQKMTGGANGMGHDLQSFIGAHGLGMSNLGQM 360

Query: 361 GDRPMGQMNNLPAVQGLPASAAAAMNGGGSAGRFIPDSISTNPYHHHHQQQQQQQQQHMA 420
           GDRPMGQMNNLPAVQGLPASAAAAMNGGGSAGRF+PDSIS NPY    QQQQQQQQQ+MA
Sbjct: 361 GDRPMGQMNNLPAVQGLPASAAAAMNGGGSAGRFMPDSISANPY-QQQQQQQQQQQQYMA 420

Query: 421 QMMNQQQHALGHQPMMYARPPPAVNYVPPPYPYPYPYPPPY-HHPYPPP-QQPEPYTYFS 480
           QMMNQQQHALGHQPMMYARPPPAVNYVPPPYPYPYPYPPPY HHPYPPP QQPEPYTYFS
Sbjct: 421 QMMNQQQHALGHQPMMYARPPPAVNYVPPPYPYPYPYPPPYHHHPYPPPQQQPEPYTYFS 480

Query: 481 DENPSSCNIM 487
           DENPSSCNIM
Sbjct: 481 DENPSSCNIM 486

BLAST of CmaCh06G014330 vs. NCBI nr
Match: XP_022936835.1 (heavy metal-associated isoprenylated plant protein 32 [Cucurbita moschata])

HSP 1 Score: 817.0 bits (2109), Expect = 8.7e-233
Identity = 464/490 (94.69%), Postives = 473/490 (96.53%), Query Frame = 0

Query: 1   MSKEEFLKIQKCVLKVNIHCDGCKQKVKKILQKIDGVFTTEIDAEQGKVTVTGNVDAAVL 60
           MSKEEFLKIQKCVLKVNIHCDGCK KVKKILQKIDGVFTTEIDAEQGKVTVTGNVDAAVL
Sbjct: 1   MSKEEFLKIQKCVLKVNIHCDGCKHKVKKILQKIDGVFTTEIDAEQGKVTVTGNVDAAVL 60

Query: 61  IKKLSKSGKHAEIWGGQPANNNKNQQNNIANQMKNMQIDNAKGGNNKGQNQKGAAANGGN 120
           +KKL+KSGKHAEIWGGQPANNNKNQQNNIANQMKNMQIDNAKGGNNKGQNQKGAAANGGN
Sbjct: 61  MKKLAKSGKHAEIWGGQPANNNKNQQNNIANQMKNMQIDNAKGGNNKGQNQKGAAANGGN 120

Query: 121 NQPKGGGQGQGLPP--VQLQQLQQMKGFQDLKLPPQFKGLKLPVKDQNANLPKGGKFNLH 180
           NQPKGGGQGQGLPP  VQLQQLQQMKGFQDLKLPPQFKGLKLPVKDQN NLPKGGKFNLH
Sbjct: 121 NQPKGGGQGQGLPPVQVQLQQLQQMKGFQDLKLPPQFKGLKLPVKDQNVNLPKGGKFNLH 180

Query: 181 EGDDLSDENDEDDYDDEDDYDDDDELDDDLGNLHLPPASKMKPMMGNGQIPKMMMNGNLP 240
           EGDDLSDE DED+Y+DEDDYDD+DELDDDLGNLHLPPASKMKPMMGNGQIPKMMMNGNLP
Sbjct: 181 EGDDLSDE-DEDEYEDEDDYDDEDELDDDLGNLHLPPASKMKPMMGNGQIPKMMMNGNLP 240

Query: 241 PVVNGPAGGGNAKKGGGAVPVQGNNNGKNVTGGGGKGNGGGGGGGGSNQKQGGGGGSNQK 300
           PVVNGPAGGGNAKKGGGAV VQGNNNGKN  GGG KGNGGGGGGGGSNQKQGGGGGSNQK
Sbjct: 241 PVVNGPAGGGNAKKGGGAVSVQGNNNGKNGNGGGSKGNGGGGGGGGSNQKQGGGGGSNQK 300

Query: 301 QGGGCGGGVSNGKNGNGGGNGSSNGVNGGQKMTGGSNGMGHDLQSFIGAHGLGMSNLGQM 360
           QGGG GGG SNGKNGNGGGNGSSNGVNGGQK+TGG+NGMGHDLQSFIGAHGLGMSNLGQM
Sbjct: 301 QGGG-GGGGSNGKNGNGGGNGSSNGVNGGQKVTGGANGMGHDLQSFIGAHGLGMSNLGQM 360

Query: 361 GDRPMGQMNNLPAVQGLPASAAAAMNGGGSAGRFIPDSISTNPYHHHHQQQQQQQQQHMA 420
           GDRPMGQMNNLPAVQGLPASAAAAMNGGGSAGRF+PDSISTNPY    QQQQQQQQQ+MA
Sbjct: 361 GDRPMGQMNNLPAVQGLPASAAAAMNGGGSAGRFMPDSISTNPY--QQQQQQQQQQQYMA 420

Query: 421 QMMNQQQHALGHQPMMYARPPPAVNYVPPPYPYPYPYPPPY-HHPYPPP-QQPEPYTYFS 480
           QMMNQQQHALGHQPMMYARPPPAVNYVPPPYPYPYPYPPPY HHPYPPP QQPEPYTYFS
Sbjct: 421 QMMNQQQHALGHQPMMYARPPPAVNYVPPPYPYPYPYPPPYHHHPYPPPQQQPEPYTYFS 480

Query: 481 DENPSSCNIM 487
           DENPSSCNIM
Sbjct: 481 DENPSSCNIM 486

BLAST of CmaCh06G014330 vs. TAIR 10
Match: AT3G06130.1 (Heavy metal transport/detoxification superfamily protein )

HSP 1 Score: 307.4 bits (786), Expect = 2.1e-83
Identity = 274/514 (53.31%), Postives = 321/514 (62.45%), Query Frame = 0

Query: 1   MSKEEFLKIQKCVLKVNIHCDGCKQKVKKILQKIDGVFTTEIDAEQGKVTVTGNVDAAVL 60
           MSKEEF+KIQ CVLKVNIHCDGCKQKVKKILQKI+GVFTT+ID+EQGKVTV+G+VD +VL
Sbjct: 1   MSKEEFMKIQTCVLKVNIHCDGCKQKVKKILQKIEGVFTTKIDSEQGKVTVSGSVDPSVL 60

Query: 61  IKKLSKSGKHAEIWGGQPANNNKNQQNNIANQMKNMQIDNAK----GGNNKGQNQKGAAA 120
           IKKL+KSGKHAEIWG    NNN N Q+ +ANQ K MQID+ K    GG N   N+KG   
Sbjct: 61  IKKLAKSGKHAEIWGAPKGNNNPN-QSQMANQFKGMQIDHGKAGGGGGGNNNNNKKGQKN 120

Query: 121 NGGNNQPKGGG------QGQGLPP---VQLQQLQQMKGFQDLKLPPQFKGLKLPVKDQNA 180
            GG     GGG       GQ L P    QLQQLQ+MKGFQDLKLPPQ KG     K+QN 
Sbjct: 121 GGGGGGGGGGGNSNAPKMGQQLNPQHLQQLQQLQKMKGFQDLKLPPQLKGSVPVNKNQN- 180

Query: 181 NLPKGGKFNLHEGDDLSDENDEDDYDDEDDYDDDDELDDDLGNLHLPPASKMKP---MMG 240
              KG KF++ E DD  D++  D++DDE   DDDDE DD+  +L L P++KMKP   MM 
Sbjct: 181 --QKGVKFDVPEDDD--DDDFSDEFDDEFTDDDDDEFDDEFDDLPL-PSNKMKPNMTMMP 240

Query: 241 NGQIPKMMMNGNLPPVVNGPAGGGNAKKGGGAVPVQGNNNGKNVTGGGGKGNGGGGGGGG 300
           N Q  +MMMN       N    GG AK GG   P  G         GGGKG GGG  GG 
Sbjct: 241 NAQ--QMMMNAQ----KNANLAGGPAKNGGKGAPAAGGGG-----AGGGKGAGGGAKGGP 300

Query: 301 SNQKQGG---GGGSNQKQG--GGCGGGVSNGKNGNGGGNGSSNGVNGGQKMTGGSNGMGH 360
            NQ QGG   GGG + + G  GG GGG + GK GNGGG   + GV+GG +  GG      
Sbjct: 301 GNQNQGGGKNGGGGHPQDGKNGGGGGGPNAGKKGNGGGGPMAGGVSGGFRPMGGG----- 360

Query: 361 DLQSFIGAHGLGMSNLGQMGDRPMGQMNNLPAVQGLPASAAAAMNGGGSAGRFIPDSIST 420
                 G   + M   GQMG    G M N+PAVQGLPA+      GG   G F    I  
Sbjct: 361 ------GPQNMSMPMGGQMG--MGGPMGNMPAVQGLPATGP----GGAPQGYFQGAGIDP 420

Query: 421 NPYHHHHQQQQQQQQQHMAQMMNQQQHALGH---QPMMYARPPPAVNYVPP---PYPYPY 480
                   QQQQQQQQ++A +MNQQ+ A+G+   QPMMYARPPPAVNY+PP    YP P+
Sbjct: 421 -----MQMQQQQQQQQYLAAVMNQQR-AMGNERFQPMMYARPPPAVNYMPPNPHQYPNPH 473

Query: 481 PYPPPYHHPYPPPQQPEPYTY-FSDENPSSCNIM 487
           PYP PY +PYPPP   + Y++ FSDEN SSC+IM
Sbjct: 481 PYPYPYPYPYPPPYGNDQYSHAFSDENTSSCDIM 473

BLAST of CmaCh06G014330 vs. TAIR 10
Match: AT5G19090.1 (Heavy metal transport/detoxification superfamily protein )

HSP 1 Score: 301.2 bits (770), Expect = 1.5e-81
Identity = 292/600 (48.67%), Postives = 333/600 (55.50%), Query Frame = 0

Query: 1   MSKEEFLKIQKCVLKVNIHCDGCKQKVKKILQKIDGVFTTEIDAEQGKVTVTGNVDAAVL 60
           MSKEEF+KIQ CVLKVNIHCDGCKQKVKKILQKI+GVFTT+IDAE GKVTV+GNVD +VL
Sbjct: 1   MSKEEFMKIQTCVLKVNIHCDGCKQKVKKILQKIEGVFTTKIDAELGKVTVSGNVDPSVL 60

Query: 61  IKKLSKSGKHAEIWGGQPANNNKNQ-QNNIANQMKNMQIDN-AKGG---------NNKGQ 120
           IKKL KSGKHAEIWG     +N NQ Q N+ANQ K MQID+  KGG         NNKGQ
Sbjct: 61  IKKLLKSGKHAEIWGAPKGGSNNNQNQPNLANQFKAMQIDHGGKGGGGGGGGPANNNKGQ 120

Query: 121 NQKGAAANGGNNQPKGGGQGQGLPP--------------------VQLQQLQQMKGFQDL 180
              G    GG     GGG G G PP                     QLQQLQQMKGFQDL
Sbjct: 121 KIGGGGGGGGG----GGGGGGGGPPKMVIPQLTPQQMQQLNPQQLQQLQQLQQMKGFQDL 180

Query: 181 KLPPQFKGLKLP-----VKDQNANLP-----KGGKFNLHEGDDLSDENDE-DDYDDEDDY 240
           KLPPQ KG   P       ++N  +P     K  KFN+ + DD  D +DE DD  DEDD 
Sbjct: 181 KLPPQLKGGPGPGPGSVPMNKNPQMPNNPNQKAVKFNVPDDDDEEDFSDEFDDEFDEDDD 240

Query: 241 DDDDELDDDLGNLHLPPASKMKPMMG-------NGQIPKMMM-----------NGNLP-- 300
           + DD+L+DD  + H PP +KMKPMMG       N  +P MMM           NG  P  
Sbjct: 241 EFDDDLEDDEFDDHPPPPNKMKPMMGGGNMIMPNNMMPNMMMPNAQQMLNAHKNGGGPGP 300

Query: 301 ------------PVVNGPAGGGNAKKGGGAVPVQGNNNGKNVTGGGGKGNGGGGGGGGSN 360
                       PV  G  GGG   K GG     GN   +N  GGGGK  G GGGG   +
Sbjct: 301 AGGKIEGKGMPFPVQMGGGGGGPGGKKGGPGGGGGNMGNQN-QGGGGKNGGKGGGGHPLD 360

Query: 361 QKQGGGGGS-NQKQGGG---CGGGVSNGKNGNGGGNGS-----SNGVNGGQKMTGGSNGM 420
            K GGGGG  N  +GGG     GG + GK G GGG G      S G+  G +  GG  G 
Sbjct: 361 GKMGGGGGGPNGNKGGGGVQMNGGPNGGKKGGGGGGGGGGGPMSGGLPPGFRPMGGGGGG 420

Query: 421 GHDLQSFI----GAHGLGMSNLGQMGDRPMGQMNNLPAVQGLPASAAAAMNGGGSAGRFI 480
           G   QS      GA G  M +L QMG  P    NN+ AVQGLPA       GGG +    
Sbjct: 421 GGGPQSMSMPMGGAMGGPMGSLPQMGGGPGPMSNNMQAVQGLPAMGPGGGGGGGPSAEAP 480

Query: 481 P---------------DSISTNPYHHHHQQQQQQQQQHMAQMMNQQQHALGH---QPMMY 487
           P               DS+  NPY    QQQQQQQQQ++A +MNQQ+ ++G+   QPMMY
Sbjct: 481 PGYFQGQVSGNGGGGQDSMPGNPY--LQQQQQQQQQQYLAAVMNQQR-SMGNERFQPMMY 540

BLAST of CmaCh06G014330 vs. TAIR 10
Match: AT5G19090.2 (Heavy metal transport/detoxification superfamily protein )

HSP 1 Score: 262.7 bits (670), Expect = 5.9e-70
Identity = 248/540 (45.93%), Postives = 296/540 (54.81%), Query Frame = 0

Query: 1   MSKEEFLKIQKCVLKVNIHCDGCKQKVKKILQKIDGVFTTEIDAEQGKVTVTGNVDAAVL 60
           MSKEEF+KIQ CVLKVNIHCDGCKQKVKKILQKI+GVFTT+IDAE GKVTV+GNVD +VL
Sbjct: 1   MSKEEFMKIQTCVLKVNIHCDGCKQKVKKILQKIEGVFTTKIDAELGKVTVSGNVDPSVL 60

Query: 61  IKKLSKSGKHAEIWGGQPANNNKNQ-QNNIANQMKNMQIDN-AKGG---------NNKGQ 120
           IKKL KSGKHAEIWG     +N NQ Q N+ANQ K MQID+  KGG         NNKGQ
Sbjct: 61  IKKLLKSGKHAEIWGAPKGGSNNNQNQPNLANQFKAMQIDHGGKGGGGGGGGPANNNKGQ 120

Query: 121 NQKGAAANGGNNQPKGGGQGQGLPP--------------------VQLQQLQQMKGFQDL 180
              G    GG     GGG G G PP                     QLQQLQQMKGFQDL
Sbjct: 121 KIGGGGGGGGG----GGGGGGGGPPKMVIPQLTPQQMQQLNPQQLQQLQQLQQMKGFQDL 180

Query: 181 KLPPQFKGLKLP-----VKDQNANLP-----KGGKFNLHEGDDLSDENDE-DDYDDEDDY 240
           KLPPQ KG   P       ++N  +P     K  KFN+ + DD  D +DE DD  DEDD 
Sbjct: 181 KLPPQLKGGPGPGPGSVPMNKNPQMPNNPNQKAVKFNVPDDDDEEDFSDEFDDEFDEDDD 240

Query: 241 DDDDELDDDLGNLHLPPASKMKPMMGNGQIPKMMMNGNLPPVVNGPAGGGNAKKGGGAVP 300
           + DD+L+DD  + H PP +KMKPMMG G    M+M  N+ P +  P    NA++   A  
Sbjct: 241 EFDDDLEDDEFDDHPPPPNKMKPMMGGG---NMIMPNNMMPNMMMP----NAQQMLNA-- 300

Query: 301 VQGNNNGKNVTGGGGKGNGGGGGGGGSNQKQGGGGGSNQKQGGGCGGGVSNGKNGNGGGN 360
                                        K GGGGG  Q      GG +       GG  
Sbjct: 301 ----------------------------HKNGGGGGGPQSMSMPMGGAM-------GGPM 360

Query: 361 GSSNGVNGGQKMTGGSNGMGHDLQSFIGAHGLGMSNLGQMGDRPMGQMNNLPAVQGLPAS 420
           GS        +M GG   M +++Q+  G   +G          P G     P+ +  P  
Sbjct: 361 GSL------PQMGGGPGPMSNNMQAVQGLPAMG----------PGGGGGGGPSAEAPPGY 420

Query: 421 AAAAMNGGGSAGRFIPDSISTNPYHHHHQQQQQQQQQHMAQMMNQQQHALGH---QPMMY 480
               ++G G  G+   DS+  NPY    QQQQQQQQQ++A +MNQQ+ ++G+   QPMMY
Sbjct: 421 FQGQVSGNGGGGQ---DSMPGNPY--LQQQQQQQQQQYLAAVMNQQR-SMGNERFQPMMY 465

Query: 481 ARPPPAVNYVPP--------PYPYPYPYPPPYHHPYPPPQQPEPYT-YFSDENPSSCNIM 487
           ARPPPAVNY+PP        PYPYPYPYPP Y     PP   + Y+ YF+DEN SSCNIM
Sbjct: 481 ARPPPAVNYMPPQPQPHQQHPYPYPYPYPPQY-----PPHNGDQYSDYFNDENTSSCNIM 465

BLAST of CmaCh06G014330 vs. TAIR 10
Match: AT5G19090.3 (Heavy metal transport/detoxification superfamily protein )

HSP 1 Score: 262.7 bits (670), Expect = 5.9e-70
Identity = 248/540 (45.93%), Postives = 296/540 (54.81%), Query Frame = 0

Query: 1   MSKEEFLKIQKCVLKVNIHCDGCKQKVKKILQKIDGVFTTEIDAEQGKVTVTGNVDAAVL 60
           MSKEEF+KIQ CVLKVNIHCDGCKQKVKKILQKI+GVFTT+IDAE GKVTV+GNVD +VL
Sbjct: 1   MSKEEFMKIQTCVLKVNIHCDGCKQKVKKILQKIEGVFTTKIDAELGKVTVSGNVDPSVL 60

Query: 61  IKKLSKSGKHAEIWGGQPANNNKNQ-QNNIANQMKNMQIDN-AKGG---------NNKGQ 120
           IKKL KSGKHAEIWG     +N NQ Q N+ANQ K MQID+  KGG         NNKGQ
Sbjct: 61  IKKLLKSGKHAEIWGAPKGGSNNNQNQPNLANQFKAMQIDHGGKGGGGGGGGPANNNKGQ 120

Query: 121 NQKGAAANGGNNQPKGGGQGQGLPP--------------------VQLQQLQQMKGFQDL 180
              G    GG     GGG G G PP                     QLQQLQQMKGFQDL
Sbjct: 121 KIGGGGGGGGG----GGGGGGGGPPKMVIPQLTPQQMQQLNPQQLQQLQQLQQMKGFQDL 180

Query: 181 KLPPQFKGLKLP-----VKDQNANLP-----KGGKFNLHEGDDLSDENDE-DDYDDEDDY 240
           KLPPQ KG   P       ++N  +P     K  KFN+ + DD  D +DE DD  DEDD 
Sbjct: 181 KLPPQLKGGPGPGPGSVPMNKNPQMPNNPNQKAVKFNVPDDDDEEDFSDEFDDEFDEDDD 240

Query: 241 DDDDELDDDLGNLHLPPASKMKPMMGNGQIPKMMMNGNLPPVVNGPAGGGNAKKGGGAVP 300
           + DD+L+DD  + H PP +KMKPMMG G    M+M  N+ P +  P    NA++   A  
Sbjct: 241 EFDDDLEDDEFDDHPPPPNKMKPMMGGG---NMIMPNNMMPNMMMP----NAQQMLNA-- 300

Query: 301 VQGNNNGKNVTGGGGKGNGGGGGGGGSNQKQGGGGGSNQKQGGGCGGGVSNGKNGNGGGN 360
                                        K GGGGG  Q      GG +       GG  
Sbjct: 301 ----------------------------HKNGGGGGGPQSMSMPMGGAM-------GGPM 360

Query: 361 GSSNGVNGGQKMTGGSNGMGHDLQSFIGAHGLGMSNLGQMGDRPMGQMNNLPAVQGLPAS 420
           GS        +M GG   M +++Q+  G   +G          P G     P+ +  P  
Sbjct: 361 GSL------PQMGGGPGPMSNNMQAVQGLPAMG----------PGGGGGGGPSAEAPPGY 420

Query: 421 AAAAMNGGGSAGRFIPDSISTNPYHHHHQQQQQQQQQHMAQMMNQQQHALGH---QPMMY 480
               ++G G  G+   DS+  NPY    QQQQQQQQQ++A +MNQQ+ ++G+   QPMMY
Sbjct: 421 FQGQVSGNGGGGQ---DSMPGNPY--LQQQQQQQQQQYLAAVMNQQR-SMGNERFQPMMY 465

Query: 481 ARPPPAVNYVPP--------PYPYPYPYPPPYHHPYPPPQQPEPYT-YFSDENPSSCNIM 487
           ARPPPAVNY+PP        PYPYPYPYPP Y     PP   + Y+ YF+DEN SSCNIM
Sbjct: 481 ARPPPAVNYMPPQPQPHQQHPYPYPYPYPPQY-----PPHNGDQYSDYFNDENTSSCNIM 465

BLAST of CmaCh06G014330 vs. TAIR 10
Match: AT3G06130.2 (Heavy metal transport/detoxification superfamily protein )

HSP 1 Score: 224.2 bits (570), Expect = 2.3e-58
Identity = 193/347 (55.62%), Postives = 220/347 (63.40%), Query Frame = 0

Query: 1   MSKEEFLKIQKCVLKVNIHCDGCKQKVKKILQKIDGVFTTEIDAEQGKVTVTGNVDAAVL 60
           MSKEEF+KIQ CVLKVNIHCDGCKQKVKKILQKI+GVFTT+ID+EQGKVTV+G+VD +VL
Sbjct: 1   MSKEEFMKIQTCVLKVNIHCDGCKQKVKKILQKIEGVFTTKIDSEQGKVTVSGSVDPSVL 60

Query: 61  IKKLSKSGKHAEIWGGQPANNNKNQQNNIANQMKNMQIDNAK----GGNNKGQNQKGAAA 120
           IKKL+KSGKHAEIWG    NNN N Q+ +ANQ K MQID+ K    GG N   N+KG   
Sbjct: 61  IKKLAKSGKHAEIWGAPKGNNNPN-QSQMANQFKGMQIDHGKAGGGGGGNNNNNKKGQKN 120

Query: 121 NGGNNQPKGGG------QGQGLPP---VQLQQLQQMKGFQDLKLPPQFKGLKLPVKDQNA 180
            GG     GGG       GQ L P    QLQQLQ+MKGFQDLKLPPQ KG     K+QN 
Sbjct: 121 GGGGGGGGGGGNSNAPKMGQQLNPQHLQQLQQLQKMKGFQDLKLPPQLKGSVPVNKNQN- 180

Query: 181 NLPKGGKFNLHEGDDLSDENDEDDYDDEDDYDDDDELDDDLGNLHLPPASKMKP---MMG 240
              KG KF++ E DD  D++  D++DDE   DDDDE DD+  +L L P++KMKP   MM 
Sbjct: 181 --QKGVKFDVPEDDD--DDDFSDEFDDEFTDDDDDEFDDEFDDLPL-PSNKMKPNMTMMP 240

Query: 241 NGQIPKMMMNGNLPPVVNGPAGGGNAKKGGGAVPVQGNNNGKNVTGGGGKGNGGGGGGGG 300
           N Q  +MMMN       N    GG AK GG   P  G         GGGKG GGG  GG 
Sbjct: 241 NAQ--QMMMNAQ----KNANLAGGPAKNGGKGAPAAGGGG-----AGGGKGAGGGAKGGP 300

Query: 301 SNQKQGGGGGSNQKQGGGCGGGVSNGKNGNGGGNGSSNGVNGGQKMT 332
            NQ QGGG     K GG  GG   +GKNG GGG     G N G+K T
Sbjct: 301 GNQNQGGG-----KNGG--GGHPQDGKNGGGGG-----GPNAGKKAT 317

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9M8K52.9e-8253.31Heavy metal-associated isoprenylated plant protein 32 OS=Arabidopsis thaliana OX... [more]
F4JZL72.1e-8048.67Heavy metal-associated isoprenylated plant protein 33 OS=Arabidopsis thaliana OX... [more]
Q0WV377.6e-3838.31Heavy metal-associated isoprenylated plant protein 34 OS=Arabidopsis thaliana OX... [more]
Q84J881.2e-1942.48Heavy metal-associated isoprenylated plant protein 36 OS=Arabidopsis thaliana OX... [more]
A2RVM86.6e-1846.97Heavy metal-associated isoprenylated plant protein 37 OS=Arabidopsis thaliana OX... [more]
Match NameE-valueIdentityDescription
A0A6J1IC551.3e-255100.00heavy metal-associated isoprenylated plant protein 32 OS=Cucurbita maxima OX=366... [more]
A0A6J1FEU14.2e-23394.69heavy metal-associated isoprenylated plant protein 32 OS=Cucurbita moschata OX=3... [more]
A0A6J1ITG71.1e-18078.70heavy metal-associated isoprenylated plant protein 32-like OS=Cucurbita maxima O... [more]
A0A6J1GW781.4e-18078.00heavy metal-associated isoprenylated plant protein 32-like OS=Cucurbita moschata... [more]
A0A5D3D1Q81.9e-18077.67WAG22 antigen OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold434G00080 ... [more]
Match NameE-valueIdentityDescription
XP_022973750.12.8e-255100.00heavy metal-associated isoprenylated plant protein 32 [Cucurbita maxima][more]
XP_023522019.14.9e-23695.50heavy metal-associated isoprenylated plant protein 32-like [Cucurbita pepo subsp... [more]
KAG7028924.11.0e-23394.51Heavy metal-associated isoprenylated plant protein 32 [Cucurbita argyrosperma su... [more]
XP_023522188.12.3e-23394.90heavy metal-associated isoprenylated plant protein 32-like [Cucurbita pepo subsp... [more]
XP_022936835.18.7e-23394.69heavy metal-associated isoprenylated plant protein 32 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
AT3G06130.12.1e-8353.31Heavy metal transport/detoxification superfamily protein [more]
AT5G19090.11.5e-8148.67Heavy metal transport/detoxification superfamily protein [more]
AT5G19090.25.9e-7045.93Heavy metal transport/detoxification superfamily protein [more]
AT5G19090.35.9e-7045.93Heavy metal transport/detoxification superfamily protein [more]
AT3G06130.22.3e-5855.62Heavy metal transport/detoxification superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.30.70.100coord: 9..81
e-value: 1.9E-21
score: 77.9
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 400..426
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 163..226
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 98..133
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 462..486
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 182..207
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 399..426
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 239..340
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 311..338
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 472..486
NoneNo IPR availablePANTHERPTHR45868HEAVY METAL-ASSOCIATED ISOPRENYLATED PLANT PROTEIN 33-RELATEDcoord: 1..486
NoneNo IPR availablePANTHERPTHR45868:SF61HEAVY METAL TRANSPORT/DETOXIFICATION DOMAIN PROTEINcoord: 1..486
IPR006121Heavy metal-associated domain, HMAPFAMPF00403HMAcoord: 14..70
e-value: 1.1E-12
score: 48.2
IPR006121Heavy metal-associated domain, HMAPROSITEPS50846HMA_2coord: 17..73
score: 11.410864
IPR006121Heavy metal-associated domain, HMACDDcd00371HMAcoord: 13..72
e-value: 3.69817E-12
score: 59.5417
IPR036163Heavy metal-associated domain superfamilySUPERFAMILY55008HMA, heavy metal-associated domaincoord: 10..75

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh06G014330.1CmaCh06G014330.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0046872 metal ion binding