CmaCh06G013380 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh06G013380
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Descriptionenoyl-CoA delta isomerase 2, peroxisomal-like
LocationCma_Chr06: 8793465 .. 8794750 (-)
RNA-Seq ExpressionCmaCh06G013380
SyntenyCmaCh06G013380
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ACGAATTGAAGCAGCTTATTGAAGAACCATGTGCACACTTGAGAGGCGTGGCAATCTCTTCATCATCACCCTCACCGGCGACGACGAGCACTGGATCGGCCCACCCTTCATTTCCTCCTTCCTCTCTCTTCTCACCCAAATCAACTCCCAAGCCACTCCCGGTTCCGTTCTGATCACCACCTCCCATGGCCGCTTCTTCTCCAATGGCTTCCACCTTCCCTGGGCTCAATCCGCTGCCTCCAAATCCGCCGCTAGAGACCGCATACTCCACATGGTTCATATCTTCAAGCCTGTCCTCGCCGCCCTTCTCTCTCTCCCCATGCCCACCATCGCCGCTGTCTCCGGCCATGCCGCCGCCGCAGGTTTTGCTCTCGCTCTTAGCCATGATTACGTCCTGATGAGGCGCGATAAAGGCGTGCTCTACATGAGCGAGGTCAGTTAACTAAATCATAATTCATAAACTTTCTTATACAAATAAACGTAATATTTATTTTAATCCAAATATGTTGTAAAAATTCTTCAAATAAACTATATGCAAAATAAGATTTAAAGGTAATATGACCATAAGTTTTTTTTTTTTTTTTTAATAAATAATTTACAATAATTTGAAGTGATAATATCTTGACCCAATTAACATAATTTAATAAGAATTCACGTTTATTAATTCAAATATTAAATAGTAACAAAGTAAATTATCATTCATAAACACATTTATACCAATCAAACAATTAAAAATTGACAATAAAATATCTAATTATTAGACACAAATTTAAAAGTGGGAGGAGTAAAACTTGTAATTTATTATTCATGACAGATTGACTTGGGTTTTACATTGCCCGATTACTTTGCGGCATTGATGAGGTCGAAGATCGGGTCAGCCTCGGTTCGTCGAGACGTGTTGCTGGCAGGGATGAAGGTGAATGGTGAAACAGCAGTGAAGTTGGGAATCACAGATGCAGCACACGACGGCGAGGAGGCACTGATGGAGGCGGCGGTGGCGATGGGGGAGAAGTTGGCAAAGAGGAAGTGGGAAGGAGAAGCTTACGCGGAGATTAGGAAGAGTCTGTATCCTGAAATGTCTCCGTTACTTAATGAACTGAGTGGAAAGCTGTAAAGAGGTGAAGAGGTAATGTAAAGGAAGTATTTGTACTCTGATGTGTCGTACAATTTCATTATCATCCATTATGGATTTTTTAGTCTGACGTTCTCCCAACTGAGCTATGAGTAATCCCCACCTTCTCGATTTGATTATGGGCTTTGGTAACTCCATAAACAATAAATTGCTT

mRNA sequence

ACGAATTGAAGCAGCTTATTGAAGAACCATGTGCACACTTGAGAGGCGTGGCAATCTCTTCATCATCACCCTCACCGGCGACGACGAGCACTGGATCGGCCCACCCTTCATTTCCTCCTTCCTCTCTCTTCTCACCCAAATCAACTCCCAAGCCACTCCCGGTTCCGTTCTGATCACCACCTCCCATGGCCGCTTCTTCTCCAATGGCTTCCACCTTCCCTGGGCTCAATCCGCTGCCTCCAAATCCGCCGCTAGAGACCGCATACTCCACATGGTTCATATCTTCAAGCCTGTCCTCGCCGCCCTTCTCTCTCTCCCCATGCCCACCATCGCCGCTGTCTCCGGCCATGCCGCCGCCGCAGGTTTTGCTCTCGCTCTTAGCCATGATTACGTCCTGATGAGGCGCGATAAAGGCGTGCTCTACATGAGCGAGATTGACTTGGGTTTTACATTGCCCGATTACTTTGCGGCATTGATGAGGTCGAAGATCGGGTCAGCCTCGGTTCGTCGAGACGTGTTGCTGGCAGGGATGAAGGTGAATGGTGAAACAGCAGTGAAGTTGGGAATCACAGATGCAGCACACGACGGCGAGGAGGCACTGATGGAGGCGGCGGTGGCGATGGGGGAGAAGTTGGCAAAGAGGAAGTGGGAAGGAGAAGCTTACGCGGAGATTAGGAAGAGTCTGTATCCTGAAATGTCTCCGTTACTTAATGAACTGAGTGGAAAGCTGTAAAGAGGTGAAGAGGTAATGTAAAGGAAGTATTTGTACTCTGATGTGTCGTACAATTTCATTATCATCCATTATGGATTTTTTAGTCTGACGTTCTCCCAACTGAGCTATGAGTAATCCCCACCTTCTCGATTTGATTATGGGCTTTGGTAACTCCATAAACAATAAATTGCTT

Coding sequence (CDS)

ATGTGCACACTTGAGAGGCGTGGCAATCTCTTCATCATCACCCTCACCGGCGACGACGAGCACTGGATCGGCCCACCCTTCATTTCCTCCTTCCTCTCTCTTCTCACCCAAATCAACTCCCAAGCCACTCCCGGTTCCGTTCTGATCACCACCTCCCATGGCCGCTTCTTCTCCAATGGCTTCCACCTTCCCTGGGCTCAATCCGCTGCCTCCAAATCCGCCGCTAGAGACCGCATACTCCACATGGTTCATATCTTCAAGCCTGTCCTCGCCGCCCTTCTCTCTCTCCCCATGCCCACCATCGCCGCTGTCTCCGGCCATGCCGCCGCCGCAGGTTTTGCTCTCGCTCTTAGCCATGATTACGTCCTGATGAGGCGCGATAAAGGCGTGCTCTACATGAGCGAGATTGACTTGGGTTTTACATTGCCCGATTACTTTGCGGCATTGATGAGGTCGAAGATCGGGTCAGCCTCGGTTCGTCGAGACGTGTTGCTGGCAGGGATGAAGGTGAATGGTGAAACAGCAGTGAAGTTGGGAATCACAGATGCAGCACACGACGGCGAGGAGGCACTGATGGAGGCGGCGGTGGCGATGGGGGAGAAGTTGGCAAAGAGGAAGTGGGAAGGAGAAGCTTACGCGGAGATTAGGAAGAGTCTGTATCCTGAAATGTCTCCGTTACTTAATGAACTGAGTGGAAAGCTGTAA

Protein sequence

MCTLERRGNLFIITLTGDDEHWIGPPFISSFLSLLTQINSQATPGSVLITTSHGRFFSNGFHLPWAQSAASKSAARDRILHMVHIFKPVLAALLSLPMPTIAAVSGHAAAAGFALALSHDYVLMRRDKGVLYMSEIDLGFTLPDYFAALMRSKIGSASVRRDVLLAGMKVNGETAVKLGITDAAHDGEEALMEAAVAMGEKLAKRKWEGEAYAEIRKSLYPEMSPLLNELSGKL
Homology
BLAST of CmaCh06G013380 vs. ExPASy Swiss-Prot
Match: O23299 (Enoyl-CoA delta isomerase 2, peroxisomal OS=Arabidopsis thaliana OX=3702 GN=ECI2 PE=1 SV=1)

HSP 1 Score: 292.0 bits (746), Expect = 6.1e-78
Identity = 144/228 (63.16%), Postives = 186/228 (81.58%), Query Frame = 0

Query: 1   MCTLERRGNLFIITLTGDDEHWIGPPFISSFLSLLTQINSQATPGSVLITTSHGRFFSNG 60
           MCTLE+RG+LF++TLTGD EH   P  I++ LSLL Q  SQ+T GS+LITT++G+FFSNG
Sbjct: 1   MCTLEKRGDLFLLTLTGDGEHRFHPDTIATILSLLEQAKSQSTRGSILITTANGKFFSNG 60

Query: 61  FHLPWAQSAASKSAARDRILHMVHIFKPVLAALLSLPMPTIAAVSGHAAAAGFALALSHD 120
           F L WAQ+A SK+ A +R+  MV  FKPV+AALL LPMPTIAA++GHAAAAG  LALSHD
Sbjct: 61  FDLAWAQTAGSKTGAANRLHQMVESFKPVVAALLDLPMPTIAALNGHAAAAGLILALSHD 120

Query: 121 YVLMRRDKGVLYMSEIDLGFTLPDYFAALMRSKIGSASVRRDVLLAGMKVNGETAVKLGI 180
           YV MR+D+GVLYMSE+D+G ++PDYF+AL+R+KIG+++ RR++LL+G K+ GE AV LGI
Sbjct: 121 YVFMRKDRGVLYMSEVDIGLSMPDYFSALVRAKIGTSAARRELLLSGKKIRGEEAVGLGI 180

Query: 181 TD-AAHDGEEALMEAAVAMGEKLAKRKWEGEAYAEIRKSLYPEMSPLL 228
            D AA+D EE ++ A+V +GEKLA +KW GE YA IRKSLYPE+  +L
Sbjct: 181 VDSAAYDSEEGVVVASVRLGEKLAAKKWSGEVYASIRKSLYPELCGIL 228

BLAST of CmaCh06G013380 vs. ExPASy Swiss-Prot
Match: O23300 (Enoyl-CoA delta isomerase 3 OS=Arabidopsis thaliana OX=3702 GN=ECI3 PE=1 SV=1)

HSP 1 Score: 280.4 bits (716), Expect = 1.8e-74
Identity = 144/235 (61.28%), Postives = 179/235 (76.17%), Query Frame = 0

Query: 1   MCTLERRGNLFIITLTGDDEHWIGPPFISSFLSLLTQINSQATPGSVLITTSHGRFFSNG 60
           MCTLE+RG+LF++TLTG+DEH   P  I+S LSLL Q  SQ+T GSVLITT HG+FFSNG
Sbjct: 1   MCTLEKRGDLFLLTLTGEDEHRFHPDTIASVLSLLEQAKSQSTKGSVLITTGHGKFFSNG 60

Query: 61  FHLPWAQSAASKSAARDRILHMVHIFKPVLAALLSLPMPTIAAVSGHAAAAGFALALSHD 120
           F L WAQSA     A  R+  MV  FKPVLAALL LPMPTIAA++GHAAA+G   ALSHD
Sbjct: 61  FDLAWAQSAG--HGAIKRMHQMVKSFKPVLAALLDLPMPTIAALNGHAAASGLMFALSHD 120

Query: 121 YVLMRRDKGVLYMSEIDLGFTLPDYFAALMRSKIGSASVRRDVLLAGMKVNGETAVKLGI 180
           YV MR+D+GVLYMSE+D+G  +PDYF+AL+ +K+GS   RR++LL+G K+ GE AV LGI
Sbjct: 121 YVFMRKDRGVLYMSEVDIGLPVPDYFSALVVAKVGSGIARRELLLSGKKLKGEEAVALGI 180

Query: 181 TD-AAHDGEEALMEAAVAMGEKLAKRKWEGEAYAEIRKSLYPEMSPLLNELSGKL 235
            D AAHD  E ++EA V++GE LA +KW GE YA IRKSLYPE+  +++  +  L
Sbjct: 181 VDSAAHDSAEGVVEATVSLGESLAAKKWNGEVYATIRKSLYPELCRMVDLTANNL 233

BLAST of CmaCh06G013380 vs. ExPASy Swiss-Prot
Match: O04469 (Enoyl-CoA delta isomerase 1, peroxisomal OS=Arabidopsis thaliana OX=3702 GN=ECI1 PE=1 SV=1)

HSP 1 Score: 204.9 bits (520), Expect = 9.9e-52
Identity = 110/224 (49.11%), Postives = 151/224 (67.41%), Query Frame = 0

Query: 1   MCTLERRGNLFIITLTGDDEHWIGPPFISSFLSLLTQINSQAT-PGSVLITTSHGRFFSN 60
           MC+LE+R  LFI+ LTGD EH + P  + S  S + QI S  +   SVLITTS G+FFSN
Sbjct: 1   MCSLEKRDRLFILKLTGDGEHRLNPTLLDSLRSTINQIRSDPSFSQSVLITTSDGKFFSN 60

Query: 61  GFHLPWAQSAASKSAARDRILHMVHIFKPVLAALLSLPMPTIAAVSGHAAAAGFALALSH 120
           G+ L  A+S  S S   D  L      + ++A L+SLPMPTIAAV+GHA+AAG  LA+SH
Sbjct: 61  GYDLALAESNPSLSVVMDAKL------RSLVADLISLPMPTIAAVTGHASAAGCILAMSH 120

Query: 121 DYVLMRRDKGVLYMSEIDLGFTLPDYFAALMRSKIGSASVRRDVLLAGMKVNGETAVKLG 180
           DYVLMRRD+G LYMSE+D+   +P +F A++R KIGS + RRDV+L   KV  +  VK+G
Sbjct: 121 DYVLMRRDRGFLYMSELDIELIVPAWFMAVIRGKIGSPAARRDVMLTAAKVTADVGVKMG 180

Query: 181 ITDAAHDGEEALMEAAVAMGEKLAKRKWEGEAYAEIRKSLYPEM 224
           I D+A+      +EAA+ +GE++ +R  +G  Y ++R+SL  E+
Sbjct: 181 IVDSAYGSAAETVEAAIKLGEEIVQRGGDGHVYGKMRESLLREV 218

BLAST of CmaCh06G013380 vs. ExPASy Swiss-Prot
Match: A8ALR7 (Carnitinyl-CoA dehydratase OS=Citrobacter koseri (strain ATCC BAA-895 / CDC 4225-83 / SGSC4696) OX=290338 GN=caiD PE=3 SV=1)

HSP 1 Score: 52.0 bits (123), Expect = 1.1e-05
Identity = 47/159 (29.56%), Postives = 79/159 (49.69%), Query Frame = 0

Query: 48  LITTSHGRFFSNGFHLPWAQSAASKSAARDRILHMVHIFKP----VLAALLSLPMPTIAA 107
           +IT    +FFS G    W   AA++  A D        F P     L  +  L  P IAA
Sbjct: 51  IITGGGEKFFSAG----WDLKAAAEGEAPDA------DFGPGGFAGLTEIFDLDKPVIAA 110

Query: 108 VSGHAAAAGFALALSHDYVLMRRDKGVLYMSEIDLGFTLPDYFAALMRSKIGSASVRRDV 167
           V+G+A   GF LAL+ D+++   D     + E  LG  +PD    L   K+   ++  ++
Sbjct: 111 VNGYAFGGGFELALAADFIVC-ADNASFALPEAKLGI-VPDSGGVLRLPKLLPPAIVNEM 170

Query: 168 LLAGMKVNGETAVKLGITDAAHDGEEALMEAAVAMGEKL 203
           L+ G +++ E A++ GI +     ++ALM++A  + ++L
Sbjct: 171 LMTGRRMDAEEALRWGIVNRV-VSQQALMDSARELAQQL 196

BLAST of CmaCh06G013380 vs. ExPASy Swiss-Prot
Match: A9MR28 (Carnitinyl-CoA dehydratase OS=Salmonella arizonae (strain ATCC BAA-731 / CDC346-86 / RSK2980) OX=41514 GN=caiD PE=3 SV=1)

HSP 1 Score: 49.7 bits (117), Expect = 5.3e-05
Identity = 47/161 (29.19%), Postives = 77/161 (47.83%), Query Frame = 0

Query: 48  LITTSHGRFFSNGFHLPWAQSAASKSAARDRILHMVHIFKP----VLAALLSLPMPTIAA 107
           +IT +  +FFS G    W   AA++  A D        F P     L  L  L  P IAA
Sbjct: 51  IITGAGEKFFSAG----WDLKAAAEGEAPDA------DFGPGGFAGLTELFDLNKPVIAA 110

Query: 108 VSGHAAAAGFALALSHDYVLMRRDKGVLYMSEIDLGFTLPDYFAALMRSKIGSASVRRDV 167
           V+G+A   GF LAL+ D+++   D     + E  LG  +PD    L   KI   ++  D+
Sbjct: 111 VNGYAFGGGFELALAADFIIC-ADHASFALPEAKLGI-VPDSGGVLRLPKILPPAIVNDM 170

Query: 168 LLAGMKVNGETAVKLGITDAAHDGEEALMEAAVAMGEKLAK 205
           ++ G ++  E A++ G+ +      E L+++A  +  +L +
Sbjct: 171 VMTGRRMTAEEALRWGVVNRVVSPHE-LLDSARELARQLVQ 198

BLAST of CmaCh06G013380 vs. ExPASy TrEMBL
Match: A0A6J1IDW4 (enoyl-CoA delta isomerase 2, peroxisomal-like OS=Cucurbita maxima OX=3661 GN=LOC111474523 PE=4 SV=1)

HSP 1 Score: 458.4 bits (1178), Expect = 1.8e-125
Identity = 234/234 (100.00%), Postives = 234/234 (100.00%), Query Frame = 0

Query: 1   MCTLERRGNLFIITLTGDDEHWIGPPFISSFLSLLTQINSQATPGSVLITTSHGRFFSNG 60
           MCTLERRGNLFIITLTGDDEHWIGPPFISSFLSLLTQINSQATPGSVLITTSHGRFFSNG
Sbjct: 1   MCTLERRGNLFIITLTGDDEHWIGPPFISSFLSLLTQINSQATPGSVLITTSHGRFFSNG 60

Query: 61  FHLPWAQSAASKSAARDRILHMVHIFKPVLAALLSLPMPTIAAVSGHAAAAGFALALSHD 120
           FHLPWAQSAASKSAARDRILHMVHIFKPVLAALLSLPMPTIAAVSGHAAAAGFALALSHD
Sbjct: 61  FHLPWAQSAASKSAARDRILHMVHIFKPVLAALLSLPMPTIAAVSGHAAAAGFALALSHD 120

Query: 121 YVLMRRDKGVLYMSEIDLGFTLPDYFAALMRSKIGSASVRRDVLLAGMKVNGETAVKLGI 180
           YVLMRRDKGVLYMSEIDLGFTLPDYFAALMRSKIGSASVRRDVLLAGMKVNGETAVKLGI
Sbjct: 121 YVLMRRDKGVLYMSEIDLGFTLPDYFAALMRSKIGSASVRRDVLLAGMKVNGETAVKLGI 180

Query: 181 TDAAHDGEEALMEAAVAMGEKLAKRKWEGEAYAEIRKSLYPEMSPLLNELSGKL 235
           TDAAHDGEEALMEAAVAMGEKLAKRKWEGEAYAEIRKSLYPEMSPLLNELSGKL
Sbjct: 181 TDAAHDGEEALMEAAVAMGEKLAKRKWEGEAYAEIRKSLYPEMSPLLNELSGKL 234

BLAST of CmaCh06G013380 vs. ExPASy TrEMBL
Match: A0A6J1FNU4 (enoyl-CoA delta isomerase 2, peroxisomal-like OS=Cucurbita moschata OX=3662 GN=LOC111447470 PE=4 SV=1)

HSP 1 Score: 452.2 bits (1162), Expect = 1.3e-123
Identity = 231/234 (98.72%), Postives = 233/234 (99.57%), Query Frame = 0

Query: 1   MCTLERRGNLFIITLTGDDEHWIGPPFISSFLSLLTQINSQATPGSVLITTSHGRFFSNG 60
           MCTLERRG+LFIITLTGDDEHWIGPPFISSFLSLLTQINSQATPGSVLITTSHGRFFSNG
Sbjct: 1   MCTLERRGDLFIITLTGDDEHWIGPPFISSFLSLLTQINSQATPGSVLITTSHGRFFSNG 60

Query: 61  FHLPWAQSAASKSAARDRILHMVHIFKPVLAALLSLPMPTIAAVSGHAAAAGFALALSHD 120
           FHLPWAQSAASKSAARDRILHMVHIFKPVLAALLSLPMPTIAAVSGHAAAAGFALALSHD
Sbjct: 61  FHLPWAQSAASKSAARDRILHMVHIFKPVLAALLSLPMPTIAAVSGHAAAAGFALALSHD 120

Query: 121 YVLMRRDKGVLYMSEIDLGFTLPDYFAALMRSKIGSASVRRDVLLAGMKVNGETAVKLGI 180
           YVLMRRDKGVLYMSEIDLGFTLPDYFAALMRSKIGSASVRRDVLLAGMKVNGETAV+LGI
Sbjct: 121 YVLMRRDKGVLYMSEIDLGFTLPDYFAALMRSKIGSASVRRDVLLAGMKVNGETAVELGI 180

Query: 181 TDAAHDGEEALMEAAVAMGEKLAKRKWEGEAYAEIRKSLYPEMSPLLNELSGKL 235
           TDAAHDG EALMEAAVAMGEKLAKRKWEGEAYAEIRKSLYPEMSPLLNELSGKL
Sbjct: 181 TDAAHDGGEALMEAAVAMGEKLAKRKWEGEAYAEIRKSLYPEMSPLLNELSGKL 234

BLAST of CmaCh06G013380 vs. ExPASy TrEMBL
Match: A0A6J1CRE1 (enoyl-CoA delta isomerase 2, peroxisomal-like OS=Momordica charantia OX=3673 GN=LOC111013487 PE=4 SV=1)

HSP 1 Score: 400.6 bits (1028), Expect = 4.5e-108
Identity = 197/234 (84.19%), Postives = 220/234 (94.02%), Query Frame = 0

Query: 1   MCTLERRGNLFIITLTGDDEHWIGPPFISSFLSLLTQINSQATPGSVLITTSHGRFFSNG 60
           MCTLE+RG++FI+TLTGD+EHWIGPPF+SS LSLL+Q+ SQAT GSVL+TTSHGRFFSNG
Sbjct: 25  MCTLEKRGDVFILTLTGDNEHWIGPPFVSSLLSLLSQVKSQATRGSVLLTTSHGRFFSNG 84

Query: 61  FHLPWAQSAASKSAARDRILHMVHIFKPVLAALLSLPMPTIAAVSGHAAAAGFALALSHD 120
           F LPWAQ+A SKSAA DR+ HMVHIFKPV+AALLSLPMPTIAAVSGHAAAAGF LAL+HD
Sbjct: 85  FDLPWAQAAGSKSAATDRLAHMVHIFKPVVAALLSLPMPTIAAVSGHAAAAGFLLALAHD 144

Query: 121 YVLMRRDKGVLYMSEIDLGFTLPDYFAALMRSKIGSASVRRDVLLAGMKVNGETAVKLGI 180
           +VLMRRDKGVLYMSE+DLG T+PDYFAALMRSKIGSASVRRDVLL GMKVNGE+AVKLGI
Sbjct: 145 FVLMRRDKGVLYMSEVDLGLTMPDYFAALMRSKIGSASVRRDVLLTGMKVNGESAVKLGI 204

Query: 181 TDAAHDGEEALMEAAVAMGEKLAKRKWEGEAYAEIRKSLYPEMSPLLNELSGKL 235
            DAAHDGEE LMEAAVAMG+KLAKRKWEG AYA+IRKSLYPEM+PLL+ L+GK+
Sbjct: 205 ADAAHDGEEGLMEAAVAMGQKLAKRKWEGGAYAQIRKSLYPEMTPLLDGLNGKM 258

BLAST of CmaCh06G013380 vs. ExPASy TrEMBL
Match: A0A1S3AXB9 (enoyl-CoA delta isomerase 2, peroxisomal-like OS=Cucumis melo OX=3656 GN=LOC103483621 PE=4 SV=1)

HSP 1 Score: 399.8 bits (1026), Expect = 7.7e-108
Identity = 198/234 (84.62%), Postives = 217/234 (92.74%), Query Frame = 0

Query: 1   MCTLERRGNLFIITLTGDDEHWIGPPFISSFLSLLTQINSQATPGSVLITTSHGRFFSNG 60
           MCTLE+RG+LFI+TLTGD+EHWIGP FIS+F+SLL QI SQAT GSVL+TTSHGRFFSNG
Sbjct: 1   MCTLEKRGSLFILTLTGDNEHWIGPTFISTFISLLAQIKSQATRGSVLLTTSHGRFFSNG 60

Query: 61  FHLPWAQSAASKSAARDRILHMVHIFKPVLAALLSLPMPTIAAVSGHAAAAGFALALSHD 120
           F LPWAQ+A SKSAARDRI+HMVHIFKP++AALLSLPMPTIAA+SGHAAAAGFALAL HD
Sbjct: 61  FDLPWAQTAPSKSAARDRIVHMVHIFKPIVAALLSLPMPTIAAISGHAAAAGFALALCHD 120

Query: 121 YVLMRRDKGVLYMSEIDLGFTLPDYFAALMRSKIGSASVRRDVLLAGMKVNGETAVKLGI 180
           YVLMRRDKGV+YMSEIDLG T+PDYFAAL+RSKI S SVRRDVLLAG KVNGETA KLGI
Sbjct: 121 YVLMRRDKGVIYMSEIDLGLTMPDYFAALVRSKISSVSVRRDVLLAGRKVNGETAAKLGI 180

Query: 181 TDAAHDGEEALMEAAVAMGEKLAKRKWEGEAYAEIRKSLYPEMSPLLNELSGKL 235
            D+ HD EEALMEAAVAMGE LA+RKWE EAYAEIRKSLYPEMSPLLNEL+GK+
Sbjct: 181 VDSVHDNEEALMEAAVAMGEMLARRKWESEAYAEIRKSLYPEMSPLLNELNGKM 234

BLAST of CmaCh06G013380 vs. ExPASy TrEMBL
Match: A0A5A7U4L1 (Enoyl-CoA delta isomerase 2, peroxisomal-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold434G001340 PE=4 SV=1)

HSP 1 Score: 399.4 bits (1025), Expect = 1.0e-107
Identity = 198/234 (84.62%), Postives = 217/234 (92.74%), Query Frame = 0

Query: 1   MCTLERRGNLFIITLTGDDEHWIGPPFISSFLSLLTQINSQATPGSVLITTSHGRFFSNG 60
           MCTLE+RG+LFI+TLTGD+EHWIGP FIS+F+SLL QI SQAT GSVL+TTSHGRFFSNG
Sbjct: 1   MCTLEKRGSLFILTLTGDNEHWIGPTFISTFISLLAQIKSQATRGSVLLTTSHGRFFSNG 60

Query: 61  FHLPWAQSAASKSAARDRILHMVHIFKPVLAALLSLPMPTIAAVSGHAAAAGFALALSHD 120
           F LPWAQ+A SKSAARDRI+HMVHIFKP++AALLSLPMPTIAA+SGHAAAAGFALAL HD
Sbjct: 61  FDLPWAQTAPSKSAARDRIVHMVHIFKPIVAALLSLPMPTIAAISGHAAAAGFALALCHD 120

Query: 121 YVLMRRDKGVLYMSEIDLGFTLPDYFAALMRSKIGSASVRRDVLLAGMKVNGETAVKLGI 180
           YVLMRRDKGV+YMSEIDLG T+PDYFAAL+RSKI S SVRRDVLLAG KVNGETA KLGI
Sbjct: 121 YVLMRRDKGVIYMSEIDLGLTMPDYFAALVRSKISSVSVRRDVLLAGRKVNGETAAKLGI 180

Query: 181 TDAAHDGEEALMEAAVAMGEKLAKRKWEGEAYAEIRKSLYPEMSPLLNELSGKL 235
            D+ HD EEALMEAAVAMGE LA+RKWE EAYAEIRKSLYPEMSPLLNEL+GK+
Sbjct: 181 IDSVHDNEEALMEAAVAMGEMLARRKWESEAYAEIRKSLYPEMSPLLNELNGKM 234

BLAST of CmaCh06G013380 vs. NCBI nr
Match: XP_022975055.1 (enoyl-CoA delta isomerase 2, peroxisomal-like [Cucurbita maxima] >XP_022975341.1 enoyl-CoA delta isomerase 2, peroxisomal-like [Cucurbita maxima])

HSP 1 Score: 458.4 bits (1178), Expect = 3.8e-125
Identity = 234/234 (100.00%), Postives = 234/234 (100.00%), Query Frame = 0

Query: 1   MCTLERRGNLFIITLTGDDEHWIGPPFISSFLSLLTQINSQATPGSVLITTSHGRFFSNG 60
           MCTLERRGNLFIITLTGDDEHWIGPPFISSFLSLLTQINSQATPGSVLITTSHGRFFSNG
Sbjct: 1   MCTLERRGNLFIITLTGDDEHWIGPPFISSFLSLLTQINSQATPGSVLITTSHGRFFSNG 60

Query: 61  FHLPWAQSAASKSAARDRILHMVHIFKPVLAALLSLPMPTIAAVSGHAAAAGFALALSHD 120
           FHLPWAQSAASKSAARDRILHMVHIFKPVLAALLSLPMPTIAAVSGHAAAAGFALALSHD
Sbjct: 61  FHLPWAQSAASKSAARDRILHMVHIFKPVLAALLSLPMPTIAAVSGHAAAAGFALALSHD 120

Query: 121 YVLMRRDKGVLYMSEIDLGFTLPDYFAALMRSKIGSASVRRDVLLAGMKVNGETAVKLGI 180
           YVLMRRDKGVLYMSEIDLGFTLPDYFAALMRSKIGSASVRRDVLLAGMKVNGETAVKLGI
Sbjct: 121 YVLMRRDKGVLYMSEIDLGFTLPDYFAALMRSKIGSASVRRDVLLAGMKVNGETAVKLGI 180

Query: 181 TDAAHDGEEALMEAAVAMGEKLAKRKWEGEAYAEIRKSLYPEMSPLLNELSGKL 235
           TDAAHDGEEALMEAAVAMGEKLAKRKWEGEAYAEIRKSLYPEMSPLLNELSGKL
Sbjct: 181 TDAAHDGEEALMEAAVAMGEKLAKRKWEGEAYAEIRKSLYPEMSPLLNELSGKL 234

BLAST of CmaCh06G013380 vs. NCBI nr
Match: XP_022942426.1 (enoyl-CoA delta isomerase 2, peroxisomal-like [Cucurbita moschata])

HSP 1 Score: 452.2 bits (1162), Expect = 2.7e-123
Identity = 231/234 (98.72%), Postives = 233/234 (99.57%), Query Frame = 0

Query: 1   MCTLERRGNLFIITLTGDDEHWIGPPFISSFLSLLTQINSQATPGSVLITTSHGRFFSNG 60
           MCTLERRG+LFIITLTGDDEHWIGPPFISSFLSLLTQINSQATPGSVLITTSHGRFFSNG
Sbjct: 1   MCTLERRGDLFIITLTGDDEHWIGPPFISSFLSLLTQINSQATPGSVLITTSHGRFFSNG 60

Query: 61  FHLPWAQSAASKSAARDRILHMVHIFKPVLAALLSLPMPTIAAVSGHAAAAGFALALSHD 120
           FHLPWAQSAASKSAARDRILHMVHIFKPVLAALLSLPMPTIAAVSGHAAAAGFALALSHD
Sbjct: 61  FHLPWAQSAASKSAARDRILHMVHIFKPVLAALLSLPMPTIAAVSGHAAAAGFALALSHD 120

Query: 121 YVLMRRDKGVLYMSEIDLGFTLPDYFAALMRSKIGSASVRRDVLLAGMKVNGETAVKLGI 180
           YVLMRRDKGVLYMSEIDLGFTLPDYFAALMRSKIGSASVRRDVLLAGMKVNGETAV+LGI
Sbjct: 121 YVLMRRDKGVLYMSEIDLGFTLPDYFAALMRSKIGSASVRRDVLLAGMKVNGETAVELGI 180

Query: 181 TDAAHDGEEALMEAAVAMGEKLAKRKWEGEAYAEIRKSLYPEMSPLLNELSGKL 235
           TDAAHDG EALMEAAVAMGEKLAKRKWEGEAYAEIRKSLYPEMSPLLNELSGKL
Sbjct: 181 TDAAHDGGEALMEAAVAMGEKLAKRKWEGEAYAEIRKSLYPEMSPLLNELSGKL 234

BLAST of CmaCh06G013380 vs. NCBI nr
Match: KAG6597380.1 (Enoyl-CoA delta isomerase 3, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 449.5 bits (1155), Expect = 1.8e-122
Identity = 229/234 (97.86%), Postives = 231/234 (98.72%), Query Frame = 0

Query: 1   MCTLERRGNLFIITLTGDDEHWIGPPFISSFLSLLTQINSQATPGSVLITTSHGRFFSNG 60
           MCTLERRG+LFIITLTGDDEHWIGPPFISSFLSLLTQINSQATPGSVLITTSHGRFFSNG
Sbjct: 1   MCTLERRGDLFIITLTGDDEHWIGPPFISSFLSLLTQINSQATPGSVLITTSHGRFFSNG 60

Query: 61  FHLPWAQSAASKSAARDRILHMVHIFKPVLAALLSLPMPTIAAVSGHAAAAGFALALSHD 120
           FHLPWAQSAASKSAARDRILHMVHIFKPV+AALLSLPMPTIAAVSGHAAAAGFALALSHD
Sbjct: 61  FHLPWAQSAASKSAARDRILHMVHIFKPVVAALLSLPMPTIAAVSGHAAAAGFALALSHD 120

Query: 121 YVLMRRDKGVLYMSEIDLGFTLPDYFAALMRSKIGSASVRRDVLLAGMKVNGETAVKLGI 180
           YVLMRRDKGVLYMSEIDLGFTLPDYFA LMRSKIGSASVRRDVLLAGMKVNGETAVKLGI
Sbjct: 121 YVLMRRDKGVLYMSEIDLGFTLPDYFATLMRSKIGSASVRRDVLLAGMKVNGETAVKLGI 180

Query: 181 TDAAHDGEEALMEAAVAMGEKLAKRKWEGEAYAEIRKSLYPEMSPLLNELSGKL 235
           TDAAHDGEEALMEAAVAMGEKLAKRKWEGEAYAEIRKSLYP MSPL NELSGKL
Sbjct: 181 TDAAHDGEEALMEAAVAMGEKLAKRKWEGEAYAEIRKSLYPGMSPLFNELSGKL 234

BLAST of CmaCh06G013380 vs. NCBI nr
Match: XP_023540657.1 (enoyl-CoA delta isomerase 2, peroxisomal-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 447.6 bits (1150), Expect = 6.7e-122
Identity = 227/234 (97.01%), Postives = 232/234 (99.15%), Query Frame = 0

Query: 1   MCTLERRGNLFIITLTGDDEHWIGPPFISSFLSLLTQINSQATPGSVLITTSHGRFFSNG 60
           MCTLERRG+LFI+TLTGDDEHWIGPP ISSFLSLLTQINSQATPGSVLITTSHGRFFSNG
Sbjct: 1   MCTLERRGDLFIVTLTGDDEHWIGPPLISSFLSLLTQINSQATPGSVLITTSHGRFFSNG 60

Query: 61  FHLPWAQSAASKSAARDRILHMVHIFKPVLAALLSLPMPTIAAVSGHAAAAGFALALSHD 120
           FHLPWAQSAASKSAA+DRILHMVHIFKPV+AALLSLPMPTIAAVSGHAAAAGFALALSHD
Sbjct: 61  FHLPWAQSAASKSAAKDRILHMVHIFKPVVAALLSLPMPTIAAVSGHAAAAGFALALSHD 120

Query: 121 YVLMRRDKGVLYMSEIDLGFTLPDYFAALMRSKIGSASVRRDVLLAGMKVNGETAVKLGI 180
           YVLMRRD+GVLYMSEIDLGFTLPDYFAALMRSKIGSASVRRDVLLAGMKVNGETAVKLGI
Sbjct: 121 YVLMRRDRGVLYMSEIDLGFTLPDYFAALMRSKIGSASVRRDVLLAGMKVNGETAVKLGI 180

Query: 181 TDAAHDGEEALMEAAVAMGEKLAKRKWEGEAYAEIRKSLYPEMSPLLNELSGKL 235
           TDAAHDG EALMEAAVAMGEKLAKRKWEGEAYAEIRKSLYPEMSPLLNELSGKL
Sbjct: 181 TDAAHDGGEALMEAAVAMGEKLAKRKWEGEAYAEIRKSLYPEMSPLLNELSGKL 234

BLAST of CmaCh06G013380 vs. NCBI nr
Match: KAG7028839.1 (Enoyl-CoA delta isomerase 3, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 435.6 bits (1119), Expect = 2.6e-118
Identity = 224/234 (95.73%), Postives = 226/234 (96.58%), Query Frame = 0

Query: 1   MCTLERRGNLFIITLTGDDEHWIGPPFISSFLSLLTQINSQATPGSVLITTSHGRFFSNG 60
           MCTLERRG+LFIITLTGDDEHWIGPPFISSFLSLLTQINSQATPGSVLITTSHGRFFSNG
Sbjct: 1   MCTLERRGDLFIITLTGDDEHWIGPPFISSFLSLLTQINSQATPGSVLITTSHGRFFSNG 60

Query: 61  FHLPWAQSAASKSAARDRILHMVHIFKPVLAALLSLPMPTIAAVSGHAAAAGFALALSHD 120
           FHLPW Q     SAARDRILHMVHIFKPV+AALLSLPMPTIAAVSGHAAAAGFALALSHD
Sbjct: 61  FHLPWVQ-----SAARDRILHMVHIFKPVVAALLSLPMPTIAAVSGHAAAAGFALALSHD 120

Query: 121 YVLMRRDKGVLYMSEIDLGFTLPDYFAALMRSKIGSASVRRDVLLAGMKVNGETAVKLGI 180
           YVLMRRDKGVLYMSEIDLGFTLPDYFAALMRSKIGSASVRRDVLLAGMKVNGETAVKLGI
Sbjct: 121 YVLMRRDKGVLYMSEIDLGFTLPDYFAALMRSKIGSASVRRDVLLAGMKVNGETAVKLGI 180

Query: 181 TDAAHDGEEALMEAAVAMGEKLAKRKWEGEAYAEIRKSLYPEMSPLLNELSGKL 235
           TDAAHDGEEALMEAAVAMGEKLA RKWEGEAYAEIRKSLYPEMSPL NELSGKL
Sbjct: 181 TDAAHDGEEALMEAAVAMGEKLATRKWEGEAYAEIRKSLYPEMSPLFNELSGKL 229

BLAST of CmaCh06G013380 vs. TAIR 10
Match: AT4G14430.1 (indole-3-butyric acid response 10 )

HSP 1 Score: 292.0 bits (746), Expect = 4.4e-79
Identity = 144/228 (63.16%), Postives = 186/228 (81.58%), Query Frame = 0

Query: 1   MCTLERRGNLFIITLTGDDEHWIGPPFISSFLSLLTQINSQATPGSVLITTSHGRFFSNG 60
           MCTLE+RG+LF++TLTGD EH   P  I++ LSLL Q  SQ+T GS+LITT++G+FFSNG
Sbjct: 1   MCTLEKRGDLFLLTLTGDGEHRFHPDTIATILSLLEQAKSQSTRGSILITTANGKFFSNG 60

Query: 61  FHLPWAQSAASKSAARDRILHMVHIFKPVLAALLSLPMPTIAAVSGHAAAAGFALALSHD 120
           F L WAQ+A SK+ A +R+  MV  FKPV+AALL LPMPTIAA++GHAAAAG  LALSHD
Sbjct: 61  FDLAWAQTAGSKTGAANRLHQMVESFKPVVAALLDLPMPTIAALNGHAAAAGLILALSHD 120

Query: 121 YVLMRRDKGVLYMSEIDLGFTLPDYFAALMRSKIGSASVRRDVLLAGMKVNGETAVKLGI 180
           YV MR+D+GVLYMSE+D+G ++PDYF+AL+R+KIG+++ RR++LL+G K+ GE AV LGI
Sbjct: 121 YVFMRKDRGVLYMSEVDIGLSMPDYFSALVRAKIGTSAARRELLLSGKKIRGEEAVGLGI 180

Query: 181 TD-AAHDGEEALMEAAVAMGEKLAKRKWEGEAYAEIRKSLYPEMSPLL 228
            D AA+D EE ++ A+V +GEKLA +KW GE YA IRKSLYPE+  +L
Sbjct: 181 VDSAAYDSEEGVVVASVRLGEKLAAKKWSGEVYASIRKSLYPELCGIL 228

BLAST of CmaCh06G013380 vs. TAIR 10
Match: AT4G14440.1 (3-hydroxyacyl-CoA dehydratase 1 )

HSP 1 Score: 280.4 bits (716), Expect = 1.3e-75
Identity = 144/235 (61.28%), Postives = 179/235 (76.17%), Query Frame = 0

Query: 1   MCTLERRGNLFIITLTGDDEHWIGPPFISSFLSLLTQINSQATPGSVLITTSHGRFFSNG 60
           MCTLE+RG+LF++TLTG+DEH   P  I+S LSLL Q  SQ+T GSVLITT HG+FFSNG
Sbjct: 1   MCTLEKRGDLFLLTLTGEDEHRFHPDTIASVLSLLEQAKSQSTKGSVLITTGHGKFFSNG 60

Query: 61  FHLPWAQSAASKSAARDRILHMVHIFKPVLAALLSLPMPTIAAVSGHAAAAGFALALSHD 120
           F L WAQSA     A  R+  MV  FKPVLAALL LPMPTIAA++GHAAA+G   ALSHD
Sbjct: 61  FDLAWAQSAG--HGAIKRMHQMVKSFKPVLAALLDLPMPTIAALNGHAAASGLMFALSHD 120

Query: 121 YVLMRRDKGVLYMSEIDLGFTLPDYFAALMRSKIGSASVRRDVLLAGMKVNGETAVKLGI 180
           YV MR+D+GVLYMSE+D+G  +PDYF+AL+ +K+GS   RR++LL+G K+ GE AV LGI
Sbjct: 121 YVFMRKDRGVLYMSEVDIGLPVPDYFSALVVAKVGSGIARRELLLSGKKLKGEEAVALGI 180

Query: 181 TD-AAHDGEEALMEAAVAMGEKLAKRKWEGEAYAEIRKSLYPEMSPLLNELSGKL 235
            D AAHD  E ++EA V++GE LA +KW GE YA IRKSLYPE+  +++  +  L
Sbjct: 181 VDSAAHDSAEGVVEATVSLGESLAAKKWNGEVYATIRKSLYPELCRMVDLTANNL 233

BLAST of CmaCh06G013380 vs. TAIR 10
Match: AT1G65520.1 (delta(3), delta(2)-enoyl CoA isomerase 1 )

HSP 1 Score: 204.9 bits (520), Expect = 7.0e-53
Identity = 110/224 (49.11%), Postives = 151/224 (67.41%), Query Frame = 0

Query: 1   MCTLERRGNLFIITLTGDDEHWIGPPFISSFLSLLTQINSQAT-PGSVLITTSHGRFFSN 60
           MC+LE+R  LFI+ LTGD EH + P  + S  S + QI S  +   SVLITTS G+FFSN
Sbjct: 1   MCSLEKRDRLFILKLTGDGEHRLNPTLLDSLRSTINQIRSDPSFSQSVLITTSDGKFFSN 60

Query: 61  GFHLPWAQSAASKSAARDRILHMVHIFKPVLAALLSLPMPTIAAVSGHAAAAGFALALSH 120
           G+ L  A+S  S S   D  L      + ++A L+SLPMPTIAAV+GHA+AAG  LA+SH
Sbjct: 61  GYDLALAESNPSLSVVMDAKL------RSLVADLISLPMPTIAAVTGHASAAGCILAMSH 120

Query: 121 DYVLMRRDKGVLYMSEIDLGFTLPDYFAALMRSKIGSASVRRDVLLAGMKVNGETAVKLG 180
           DYVLMRRD+G LYMSE+D+   +P +F A++R KIGS + RRDV+L   KV  +  VK+G
Sbjct: 121 DYVLMRRDRGFLYMSELDIELIVPAWFMAVIRGKIGSPAARRDVMLTAAKVTADVGVKMG 180

Query: 181 ITDAAHDGEEALMEAAVAMGEKLAKRKWEGEAYAEIRKSLYPEM 224
           I D+A+      +EAA+ +GE++ +R  +G  Y ++R+SL  E+
Sbjct: 181 IVDSAYGSAAETVEAAIKLGEEIVQRGGDGHVYGKMRESLLREV 218

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O232996.1e-7863.16Enoyl-CoA delta isomerase 2, peroxisomal OS=Arabidopsis thaliana OX=3702 GN=ECI2... [more]
O233001.8e-7461.28Enoyl-CoA delta isomerase 3 OS=Arabidopsis thaliana OX=3702 GN=ECI3 PE=1 SV=1[more]
O044699.9e-5249.11Enoyl-CoA delta isomerase 1, peroxisomal OS=Arabidopsis thaliana OX=3702 GN=ECI1... [more]
A8ALR71.1e-0529.56Carnitinyl-CoA dehydratase OS=Citrobacter koseri (strain ATCC BAA-895 / CDC 4225... [more]
A9MR285.3e-0529.19Carnitinyl-CoA dehydratase OS=Salmonella arizonae (strain ATCC BAA-731 / CDC346-... [more]
Match NameE-valueIdentityDescription
A0A6J1IDW41.8e-125100.00enoyl-CoA delta isomerase 2, peroxisomal-like OS=Cucurbita maxima OX=3661 GN=LOC... [more]
A0A6J1FNU41.3e-12398.72enoyl-CoA delta isomerase 2, peroxisomal-like OS=Cucurbita moschata OX=3662 GN=L... [more]
A0A6J1CRE14.5e-10884.19enoyl-CoA delta isomerase 2, peroxisomal-like OS=Momordica charantia OX=3673 GN=... [more]
A0A1S3AXB97.7e-10884.62enoyl-CoA delta isomerase 2, peroxisomal-like OS=Cucumis melo OX=3656 GN=LOC1034... [more]
A0A5A7U4L11.0e-10784.62Enoyl-CoA delta isomerase 2, peroxisomal-like OS=Cucumis melo var. makuwa OX=119... [more]
Match NameE-valueIdentityDescription
XP_022975055.13.8e-125100.00enoyl-CoA delta isomerase 2, peroxisomal-like [Cucurbita maxima] >XP_022975341.1... [more]
XP_022942426.12.7e-12398.72enoyl-CoA delta isomerase 2, peroxisomal-like [Cucurbita moschata][more]
KAG6597380.11.8e-12297.86Enoyl-CoA delta isomerase 3, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_023540657.16.7e-12297.01enoyl-CoA delta isomerase 2, peroxisomal-like [Cucurbita pepo subsp. pepo][more]
KAG7028839.12.6e-11895.73Enoyl-CoA delta isomerase 3, partial [Cucurbita argyrosperma subsp. argyrosperma... [more]
Match NameE-valueIdentityDescription
AT4G14430.14.4e-7963.16indole-3-butyric acid response 10 [more]
AT4G14440.11.3e-7561.283-hydroxyacyl-CoA dehydratase 1 [more]
AT1G65520.17.0e-5349.11delta(3), delta(2)-enoyl CoA isomerase 1 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001753Enoyl-CoA hydratase/isomerasePFAMPF00378ECH_1coord: 29..206
e-value: 3.1E-19
score: 69.3
NoneNo IPR availableGENE3D3.90.226.10coord: 1..230
e-value: 1.9E-36
score: 127.4
NoneNo IPR availablePANTHERPTHR11941ENOYL-COA HYDRATASE-RELATEDcoord: 1..228
NoneNo IPR availablePANTHERPTHR11941:SF75ENOYL-COA DELTA ISOMERASE 2, PEROXISOMALcoord: 1..228
NoneNo IPR availableCDDcd06558crotonase-likecoord: 2..202
e-value: 2.4453E-16
score: 72.5932
IPR029045ClpP/crotonase-like domain superfamilySUPERFAMILY52096ClpP/crotonasecoord: 3..206

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh06G013380.1CmaCh06G013380.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006635 fatty acid beta-oxidation
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0004165 dodecenoyl-CoA delta-isomerase activity
molecular_function GO:0004300 enoyl-CoA hydratase activity
molecular_function GO:0003824 catalytic activity