CmaCh06G010960 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh06G010960
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
DescriptionN-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein
LocationCma_Chr06: 7399424 .. 7406114 (-)
RNA-Seq ExpressionCmaCh06G010960
SyntenyCmaCh06G010960
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CTTCAAAGCGTACAGTGCCGACGTTTGACTCGCCGATTCTAACTACGGCTGCCCGATCGCCGGAACTCGTTCTTCCGCTTTACATTGTTGTTTGTCGTGAGAAATGGCTGCCGAGGCTCAAGATCAGGGCCATAGATTTTTCGTTGCTGTTCATGTTGGCGCGGGATTTCACGCACCTTCCAACGAGAAAGCCCTCAGGTCGGCCATGAAACGCGCTTGTTTAGCTGCCTCTCTAGTTCTCCGAAAGGTTTGTTTCACAATCTGTCAACTCAATCAAACACAAAGACTGATGCATTTTCGATTTTCACTAGATGGAAAGTTGAAACTAGGATTACTGGATTTTTAGATTTTCGCATTTCCCCTATCCAATTGATTGTGTTAAACGAGCCGGATTCCTTGTTCAGCGTGATTGTAGATATAAATTTTGTTGGAATGTATCCTGCTCTGGAGAAGTTCACGAGCATGGCGACCTCTTTGTATTCTTCTTTTTGTATGATTTTTATTTGAAGTTCGTCTATGTTTTCGTCGCTGCATTGTTGGTCTGTATCGAGTGTTGCTTCCTGTTGAATAATTTTCAATTGAAGTTTGTGAATGTCTTCTGCATTGCATTTTTTTTGGCATGTATCGAGTTATTTGGAAGTTCACGAGGACGACCACTGCCTATTCTTCTTTTCGAATCATTTCAATTGGAAATATCTTCTTCATTGCACTGTTGGCATGTATGGAGTTATAGAGAAGTGCATGAGATGGCGAGAAATTCATATTCTTCTTTCGAATAATTTGTATTTGAAGTTTGTCGGTATCTTCTTCGTTATAGCCTTATAAAATCCTTCCATTATTCTGACTTGTTTCAGGGCTCAGGCGGTTGTTTAGATGCTGTTTCCGCAGCTATACAAGTTTTAGAGGTAATTTTCTCTGTCAAATATCTTATTTCATTTCGTTTTCTCATAGTGCTGGAGTGTAATTTTACCTTTGGTAATGTATGGTTTGTATCATATGAATACCTATTCTATATCTGGAATGAAAATCATCATACCATAGTTGGACTACTGAATTCGGATGATTTGTAGTGAAACAAAGTGTTCATACCAGTCTTACTGGGATCGTGGCGTTTGGTGGTACCATTTCCATTACTTTGCGGTTGTTGGTTCACTAATTGTTTTATCTACACGACGAATTAGCTGTACTAGTACCTATAAAAGTGCTATAATGTTATAATATGACGACCTACTGTTCTCTGAAACTCTTAAAGCTTCTATTTGTATAATTTACATTTAAAGAGAGAATTTATTGTAGGATGACCCAGTGACAAATGCCGGACGAGGATCCAATTTGACCGAAGATGGTCGTGTAGAGTGTGATGCTAGTATTATGGATGGTGATACAGGTGCTTTTGGAGCTGTTGGTGCAGTGCCAGGTTTAACATTCTCTAATGATGAAACTATTTATTATCAATTATCAGCATTGGCTTCTCGGTTATTTCGATCTCCATATATTATGTGTGACTATATCTTTGCACATTTTAATAGTACTGCTACTGTACTTATTTTGTTAACTTAATTGATATCTATCATATGTTCTAGATCAGGTGTCCGAAATGCTATAAAAATCGCTGCTTTACTGGCAAAGGAACAAATATTGGGATCGTCTCTGCTGGGCCTTATACCTCCGATGTAATTCTTATTGTGAGATTGCATTGCTTTATTATCTTTCCTTGCAATGGCTCTAGCATTTTTCCTATAATAGGGTATTTTTCGCGTGTGTTAGACATTAGTCGGTCTGTCTCACGAAATTACAGAGAAAATCAGCATGGAAACAATGAGAACAGTCCCCAGCACACAATAATCTCAGGGCTTCAAGAGGCCGAGTGCTGTCCTTCCTAGAGTAAGCTCCAGTCAGAAAAAATCTTACTAGCTGAATGTCCATGCGTGGATATAACCCACAAATGAAATACCAGTCCTCCCTACAGCGCCTATTGCCACATCACCCTATTACAAAAACTGGACCACCCTCAAAATGCACATTCACAACACACTTATTCCAACTAACTAACCGTTGACTCGTCCCCTCCTTTGCGACCTGCCTCCCTCTTCTTGTAAGTTAAGGGTGTTGAGGGCCTCGCCTTCTCATCATCATGCGAGTTGGTTTCAAGTTTAGCAAAGGATGATGCCATTGCCATTACTATTCTTTATTTTTTGCTTTGTTTATTTTCCTACTTCGATTCTAAACAATTGTGGAAAACAGTGTACTGTTTTGTTTTTATCAAGATTTGATGTGGTATTGCTCTTGAAAATGTAGATTGCAGAGTATGTAACAACTAGTATGCTGCTTCATTCTTGAATTTCTAATTTCTAAAATCTAATCACCAGGAAGGTTTTCCTTGTGCAGCTTCCTAGTTGGTGAGGGTGCTTGTACTTGGGCAAAGTCCAAAGGCATTTGGTTGCCAGAAACTACTGCAGAGGCTAATGAGGTTATATGAGTAACTAAGGGACCGTTTGATAATCGTTTCAATTTCTGTATTCTGTTTTTGTTTTTCATTTTAAAAACCAGAGTTGTGTGATAACCAATTTTGTTTTTTGTTTTATGTTTTTTAGAAGCAATTTTTTTTAAACAAATGTGGAATTCATTTTTCTTCTACTTCTCATTTTCTTCCTCTTTGACACATCTTTCTCCAACAAATTCATTTTTCTTACTTTTTTTCTTTCCAAAACTTTTTTCCCTATATTTCTCTCTACAAAATTTTTGTCTCTCTCTACCAATTGTCTTCTCGATATAATTTTTCTAATTTCTCTCTCTAAACTTTTTTTTTTCTCTTTATCTCATCTATTTCTAAAATTTCTCCCCAACTTATCTACTTCTAAAATTTTTCCCTAACATTTCTCTCTCTAATAAATGTATCTCTAACTTTTCTTTTGAGAAAACATAGCTCCCTAAATTTTCTCTTGCTATAACTTTTCTTTATTTTTCTCATTTATCTTTATTTCTCTTACATATTTTTTATGCTTAACAATACTATTTCTAAAATTTCTCCTTTTATTTTAAATTTTCTCTCTCCAAATTCATCTCTTCCCATCTAACTTCTTTTTTCTTTATAACTAATATTTTTTTTTATGAAGTCTAATTAATCTTCTCTCACTAAATATTTTCTTTTATCTCTAAATTTACCATCTTTAACCTTTCTTTCCGAAACATTTTTATTGGTCTCTAATTTTTTTTTTTTTCATCTCTGATTTTTTAAAATCAGGAAACCAAAAATAAAACAATTATTGAACATATATGGTTTTTGTTTTTAAAAAACAAAAAAACCAAAAACTGAAAATTGAAATGGTTGTCAAAAGGGCCCAAGTAAAAACCCTTTGATTTATCTAAGCATTAAATTAACCATCTATATCTTACATTGTTGGGTGAATCATCTCATCTTTTACCATCACATATAGTGGTTGGTGACAGAAAGAGCAAAAGTTCAATGGGAAAGATACAAGTCAATGATTCAACGTGCGAAAGAAAAGAAAGAGAATTCTTATGTAGTGAATTCTTGCAGTTCTCAAGACAATACTAGAATATCGGGTTTGGGTTCTCTATTTTATATGTTATCCTATTTTAACCATCTCCTCTCGCCTGTTCCTAATGACTTTTATTTATTTTTATTTTGATTGTGTAGAAGTCCAATCACACAATCCACTGGAAGGCACCACATGCCAGTCAGCAACGTTAAATGATGCAGAAAATGATTGTACTATGGACACGGTTGGTGTTATATGTGTTGACAGTGAAGGACATGTCGCATCTGGGGCCTCAAGTGGTGGCATTGCAATGAAGGTAGTTTGTGCTTTTCCTCATATTATTTGAACGAGGTAGAAGTATAATTGCATGATTAGAAAAGGATTTTAACAGATTTTAACTTTTAGGTCAGTGGACGTGTAGGATTGGCAGCAATGTATGGTTCAGGTTGTTGGGCGTCCTCAAAAGGTCCATTTGGGACTCCCTTCATGGTTGGGGGTTGTGTTTCTGGTGGAGGAGAGTATCTAATGAAAGGATTTGCAGCTAGGGAATGCTGTGTATCATTATCACTGTATGTTTCTCCACTATCCATGATTAAGTTTCAGTGAATTTTCAGTGGTAAAACACTTCAATATTTTGAGGTTTCATTTTATTTATTCTGAAATTGCTCATCCAAAATGCACAAACACAGACTCCGGAACTTTGACCTCAACCTAAAGATTTCTATAACTGTACATCGTAGAACCAAGCCTAATCATCTGTGTTCGTTGTTTAATTGTTGTCAATGTACGTGAATGATTTTCTTATGATGTTCTCATATTAGAACATCAACCATATGGTTTCTATAGGTGAGGTCTTTGTGCAGCGTGGAGGACTTTATTCTATAGAATGTTAGCAATATGTATTAACAACAAAATCATAATGGGTTGGTCTTGCGATAAAAAAAGACCATGTAAATAATAAAGGCTTTGAGGAAATGAGATCAAGTCATGGTGATGATCTACTTAGGAAGTTATTAGTTGAAGGATTCTCTAGAAGGAAAAATTTGTATAATGCATATTACCGGTCTATTTATTTCACTTTCACATCGATTATCTAATGGTAACACTAATATCAATAACTTTTGGTTTGTTTATTTCAAGCGAGCACCCAAGCTGAAGTTTGAATGTGCATATGGAGTGTTTAAATTTCTGAAATAATTTGAACCAAGCAACCGTGTGTATATTGAATCTGGAAAGTAGTTAATATATTAAGTTTAGTCAAGTTTGCTCCCTGATAACGTTGGTCGATGAGATAATGACCATCTAAAAATCTTTCTTCATTCGTTGCATTTATTCTTGATCAGGTCTTATTGTTTGCACATTGAAGAATATTTGCTTACAAATTAACTAAGTTGAGTCGTCTGTTATTTTTATTTAACATTGTTATATATGGTCCACTCAGATCACAGGCAGGTCCGGCCTCTGCTTGCATGAAAGTTCTACGCAATTTTGTTCAAGAAAGCAGTCAACATAACACTGATAAGAGTGCTGGGATTTTACTTGTCCAGGCCGATGCTCCTAAATTGGTGACCTACTAATTGAACACTTTTTTTTATTCTATTTATCGTAATTATGTATCTCAGTATTGTTGCCACAGGCCCCAGGAAATCCGCAGAAGTTGAAAGCCGTGGAGATTGCTGCTGCTTATTCTTCCCTTTCATTTGGAATGGGTTACTTTGCAAGCTCCATGGAACGACCCAAGGTATTTATTCACAGTTTTCTGATTCTGTGATGATGTTAGAATCCAAGCTGTTTAATTTGGATAAGGCCTTAAGGTAAGGGGCTATATGCACTACCTTTTGTTTTCTATTTTTTAGAGTAGAGGGAGACTAAGCTACGTTAGCTATGTTGGTTCCTAAAGTCAGTCTCTCAAAAAATGGGATCGGTTGGACAACTTTGTGATGATCGCCAAAGCTATCCCATCTTGACTCCGACTTCCTATGTGTAAGGATCTACACACGCATAATGTCATAGCTCTAGGGTGCCGCCTTAAACGTGATTTGGCTTATAGTTAAGTGGATCTACCCTGTGTTTGAAGGGAGGAGTGAAAGGTGAACAAAAACTTATAATAACAAACAATCACGGAACAAGTAGAGAGGGAAAAAAAGGTATAAGTTTTAGAGGGAGATGTTAGAGAGAGGGAATGAAAGTAGACAGAAATTGGAGAGAGATCTGTTAGAGAGAGGGAGAGCCGTGAGATCTCACATCGGTTGGGGAGGAGAACGAAGCATTCTTTATTAGGAAACCTCTCTCTAGCAGACACGTTTTAAAAACTTTGAGGGGAAGTCTGAAAGTGAAAGCTCAAAGAGGACAATATCTGCTATTGGTAAACTTGGGCCGTTACAAGAGCAATGTTATAAAGAGAAATGTTACAGAGGTTAGAAAAAGAATGAGGAGCACAGGGAAACAAAAGCTATAGGAAGAGAGATATTAGAGATGGAAATAAATGTTAGAGAGAAAAGTTAGGCAGAAATATGACAGAAAAGGAAGGAGAAGTCCTTGAGTGAGAACTGTTTAGAGAGAAAAATTAGTAGTAGATATTCTAGAGAAAGATATGTTGGAGACGATATGTTAGAGAAAGGAGACTATTTGTGAATATTTCAAACAATCATATTTGTGAAACCTTTGGTTGATTATTAAATCCTCTCTTTTCTTCTCTCAGGTGTCTATTTTGAGAAGTACTAAACAAAACAATAAGATGGGAATTGATCATTTTGAAGCATGTATCGATCTTTCGGACAACGGGAAGCTCTAACACAGCTATAGCCAGAACGAGGTGCAAGAATTACGCTCTCTCCATCTTCGTCACAATCCAATTCTACAGAGAAAAGGTCATAATTGTAGAACTTCCATTACGTTTTTGTTTATTCCAGTAACAACTTGAAGATGCAGATTAGACTAGAAAGTTAGGTTCAGATCAGAAGCTCTTCACTCATGTTTCCTCCAAATTTGAGGTTGCAGTTTCTAATTTCGGGGATGGATACACAAAGGTCAAAAGAAGTTAAAGTTGGTACCATATTTATTTAGCTATACCTAATAACGAGCATATTCTGAAATATGTTTGATCCCCTCCCCTCTGCCATCATCTTTAATGATATTGATAATTAGTGGCATGTTTGAAAGTGATTTTGAGAGCTAGAGCTCTAGAC

mRNA sequence

CTTCAAAGCGTACAGTGCCGACGTTTGACTCGCCGATTCTAACTACGGCTGCCCGATCGCCGGAACTCGTTCTTCCGCTTTACATTGTTGTTTGTCGTGAGAAATGGCTGCCGAGGCTCAAGATCAGGGCCATAGATTTTTCGTTGCTGTTCATGTTGGCGCGGGATTTCACGCACCTTCCAACGAGAAAGCCCTCAGGTCGGCCATGAAACGCGCTTGTTTAGCTGCCTCTCTAGTTCTCCGAAAGGGCTCAGGCGGTTGTTTAGATGCTGTTTCCGCAGCTATACAAGTTTTAGAGGATGACCCAGTGACAAATGCCGGACGAGGATCCAATTTGACCGAAGATGGTCGTGTAGAGTGTGATGCTAGTATTATGGATGGTGATACAGGTGCTTTTGGAGCTGTTGGTGCAGTGCCAGGTGTCCGAAATGCTATAAAAATCGCTGCTTTACTGGCAAAGGAACAAATATTGGGATCGTCTCTGCTGGGCCTTATACCTCCGATCTTCCTAGTTGGTGAGGGTGCTTGTACTTGGGCAAAGTCCAAAGGCATTTGGTTGCCAGAAACTACTGCAGAGGCTAATGAGTGGTTGGTGACAGAAAGAGCAAAAGTTCAATGGGAAAGATACAAGTCAATGATTCAACGTGCGAAAGAAAAGAAAGAGAATTCTTATGTAGTGAATTCTTGCAGTTCTCAAGACAATACTAGAATATCGGAAGTCCAATCACACAATCCACTGGAAGGCACCACATGCCAGTCAGCAACGTTAAATGATGCAGAAAATGATTGTACTATGGACACGGTTGGTGTTATATGTGTTGACAGTGAAGGACATGTCGCATCTGGGGCCTCAAGTGGTGGCATTGCAATGAAGGTCAGTGGACGTGTAGGATTGGCAGCAATGTATGGTTCAGGTTGTTGGGCGTCCTCAAAAGGTCCATTTGGGACTCCCTTCATGGTTGGGGGTTGTGTTTCTGGTGGAGGAGAGTATCTAATGAAAGGATTTGCAGCTAGGGAATGCTGTGTATCATTATCACTATCACAGGCAGGTCCGGCCTCTGCTTGCATGAAAGTTCTACGCAATTTTGTTCAAGAAAGCAGTCAACATAACACTGATAAGAGTGCTGGGATTTTACTTGTCCAGGCCGATGCTCCTAAATTGGCCCCAGGAAATCCGCAGAAGTTGAAAGCCGTGGAGATTGCTGCTGCTTATTCTTCCCTTTCATTTGGAATGGGTTACTTTGCAAGCTCCATGGAACGACCCAAGGTGTCTATTTTGAGAAGTACTAAACAAAACAATAAGATGGGAATTGATCATTTTGAAGCATGTATCGATCTTTCGGACAACGGGAAGCTCTAACACAGCTATAGCCAGAACGAGGTGCAAGAATTACGCTCTCTCCATCTTCGTCACAATCCAATTCTACAGAGAAAAGGTCATAATTGTAGAACTTCCATTACGTTTTTGTTTATTCCAGTAACAACTTGAAGATGCAGATTAGACTAGAAAGTTAGGTTCAGATCAGAAGCTCTTCACTCATGTTTCCTCCAAATTTGAGGTTGCAGTTTCTAATTTCGGGGATGGATACACAAAGGTCAAAAGAAGTTAAAGTTGGTACCATATTTATTTAGCTATACCTAATAACGAGCATATTCTGAAATATGTTTGATCCCCTCCCCTCTGCCATCATCTTTAATGATATTGATAATTAGTGGCATGTTTGAAAGTGATTTTGAGAGCTAGAGCTCTAGAC

Coding sequence (CDS)

ATGGCTGCCGAGGCTCAAGATCAGGGCCATAGATTTTTCGTTGCTGTTCATGTTGGCGCGGGATTTCACGCACCTTCCAACGAGAAAGCCCTCAGGTCGGCCATGAAACGCGCTTGTTTAGCTGCCTCTCTAGTTCTCCGAAAGGGCTCAGGCGGTTGTTTAGATGCTGTTTCCGCAGCTATACAAGTTTTAGAGGATGACCCAGTGACAAATGCCGGACGAGGATCCAATTTGACCGAAGATGGTCGTGTAGAGTGTGATGCTAGTATTATGGATGGTGATACAGGTGCTTTTGGAGCTGTTGGTGCAGTGCCAGGTGTCCGAAATGCTATAAAAATCGCTGCTTTACTGGCAAAGGAACAAATATTGGGATCGTCTCTGCTGGGCCTTATACCTCCGATCTTCCTAGTTGGTGAGGGTGCTTGTACTTGGGCAAAGTCCAAAGGCATTTGGTTGCCAGAAACTACTGCAGAGGCTAATGAGTGGTTGGTGACAGAAAGAGCAAAAGTTCAATGGGAAAGATACAAGTCAATGATTCAACGTGCGAAAGAAAAGAAAGAGAATTCTTATGTAGTGAATTCTTGCAGTTCTCAAGACAATACTAGAATATCGGAAGTCCAATCACACAATCCACTGGAAGGCACCACATGCCAGTCAGCAACGTTAAATGATGCAGAAAATGATTGTACTATGGACACGGTTGGTGTTATATGTGTTGACAGTGAAGGACATGTCGCATCTGGGGCCTCAAGTGGTGGCATTGCAATGAAGGTCAGTGGACGTGTAGGATTGGCAGCAATGTATGGTTCAGGTTGTTGGGCGTCCTCAAAAGGTCCATTTGGGACTCCCTTCATGGTTGGGGGTTGTGTTTCTGGTGGAGGAGAGTATCTAATGAAAGGATTTGCAGCTAGGGAATGCTGTGTATCATTATCACTATCACAGGCAGGTCCGGCCTCTGCTTGCATGAAAGTTCTACGCAATTTTGTTCAAGAAAGCAGTCAACATAACACTGATAAGAGTGCTGGGATTTTACTTGTCCAGGCCGATGCTCCTAAATTGGCCCCAGGAAATCCGCAGAAGTTGAAAGCCGTGGAGATTGCTGCTGCTTATTCTTCCCTTTCATTTGGAATGGGTTACTTTGCAAGCTCCATGGAACGACCCAAGGTGTCTATTTTGAGAAGTACTAAACAAAACAATAAGATGGGAATTGATCATTTTGAAGCATGTATCGATCTTTCGGACAACGGGAAGCTCTAA

Protein sequence

MAAEAQDQGHRFFVAVHVGAGFHAPSNEKALRSAMKRACLAASLVLRKGSGGCLDAVSAAIQVLEDDPVTNAGRGSNLTEDGRVECDASIMDGDTGAFGAVGAVPGVRNAIKIAALLAKEQILGSSLLGLIPPIFLVGEGACTWAKSKGIWLPETTAEANEWLVTERAKVQWERYKSMIQRAKEKKENSYVVNSCSSQDNTRISEVQSHNPLEGTTCQSATLNDAENDCTMDTVGVICVDSEGHVASGASSGGIAMKVSGRVGLAAMYGSGCWASSKGPFGTPFMVGGCVSGGGEYLMKGFAARECCVSLSLSQAGPASACMKVLRNFVQESSQHNTDKSAGILLVQADAPKLAPGNPQKLKAVEIAAAYSSLSFGMGYFASSMERPKVSILRSTKQNNKMGIDHFEACIDLSDNGKL
Homology
BLAST of CmaCh06G010960 vs. ExPASy Swiss-Prot
Match: O65268 (Putative threonine aspartase OS=Arabidopsis thaliana OX=3702 GN=At4g00590 PE=2 SV=3)

HSP 1 Score: 514.6 bits (1324), Expect = 1.0e-144
Identity = 275/416 (66.11%), Postives = 330/416 (79.33%), Query Frame = 0

Query: 1   MAAEAQDQGHRFFVAVHVGAGFHAPSNEKALRSAMKRACLAASLVLRKGSGGCLDAVSAA 60
           MA +  DQ  RFFVAVHVGAG+HA +NEKALRS M+RACLAAS +LR+ SG C+DAVSAA
Sbjct: 2   MAGDGADQSRRFFVAVHVGAGYHAAANEKALRSVMRRACLAASTILRQDSGECIDAVSAA 61

Query: 61  IQVLEDDPVTNAGRGSNLTEDGRVECDASIMDGDTGAFGAVGAVPGVRNAIKIAALLAKE 120
           IQVLEDDP TNAGRGSNLTEDG VECDAS+MDGD+G FG VGAVPGVRNAIKIAALL KE
Sbjct: 62  IQVLEDDPSTNAGRGSNLTEDGHVECDASLMDGDSGMFGGVGAVPGVRNAIKIAALLVKE 121

Query: 121 QILGSSLLGLIPPIFLVGEGACTWAKSKGIWLPETTAEANEWLVTERAKVQWERYKSMIQ 180
           QI GS+LLG IPP+ LVGEGA  W KSK + +P T  EA++WLVTERA+ QW R+K+M+ 
Sbjct: 122 QITGSTLLGRIPPMLLVGEGARRWGKSKSVLIPGTVTEADQWLVTERARNQWRRFKAMLS 181

Query: 181 RAKEKKENSYVVNSCSSQDNTRISEVQSHNPLEGTTCQS--ATLNDAENDCTMDTVGVIC 240
               K       +  S++++ R +E          TC+   ++   A+ D  MDTVGVIC
Sbjct: 182 EVGAK-------SILSAEEHPRGTE-------NNETCEENVSSCAAADEDKIMDTVGVIC 241

Query: 241 VDSEGHVASGASSGGIAMKVSGRVGLAAMYGSGCWASSKGPFGTPFMVGGCVSGGGEYLM 300
           VD+EGH+A G+SSGGIAMK+SGRVGLAA YGSGCWASSKGPFG PF+VG CVSG GEYLM
Sbjct: 242 VDNEGHIACGSSSGGIAMKISGRVGLAATYGSGCWASSKGPFGAPFLVGCCVSGAGEYLM 301

Query: 301 KGFAARECCVSLSLSQAGPASACMKVLRNFV-QESSQHNT-DKSAGILLVQADAPKLAPG 360
           +GFAARECC SL+LSQAGPASA MKVLR+ + QESS+  T DK+AGIL+VQADA  + PG
Sbjct: 302 RGFAARECCTSLALSQAGPASAAMKVLRSVMHQESSKIGTADKTAGILVVQADASVVVPG 361

Query: 361 NPQKLKAVEIAAAYSSLSFGMGYFASSMERPKVSILRSTKQNNKMGIDHFEACIDL 413
           +  +L AVEIAAAYSSLSFG+GY+ +S+E+PK+SILR+ +Q ++ G+DHFEA IDL
Sbjct: 362 SKPELNAVEIAAAYSSLSFGVGYYGNSIEKPKISILRTRRQMSEAGVDHFEARIDL 403

BLAST of CmaCh06G010960 vs. ExPASy Swiss-Prot
Match: Q9H6P5 (Threonine aspartase 1 OS=Homo sapiens OX=9606 GN=TASP1 PE=1 SV=1)

HSP 1 Score: 180.3 bits (456), Expect = 4.6e-44
Identity = 141/386 (36.53%), Postives = 201/386 (52.07%), Query Frame = 0

Query: 13  FVAVHVGAGFHAPSNEKALRSAMKRACLAASLVLRKGSGGCLDAVSAAIQVLEDDPVTNA 72
           FV VH GAG+H+ S  K  +   KRAC  A   L+ G+    DAV+AA+  LED P TNA
Sbjct: 43  FVLVHAGAGYHSESKAKEYKHVCKRACQKAIEKLQAGA-LATDAVTAALVELEDSPFTNA 102

Query: 73  GRGSNLTEDGRVECDASIMDGDTGAFGAVGAVPGVRNAIKIAALLAKEQILGSSLLGLIP 132
           G GSNL   G +ECDASIMDG +  FGAVGA+ G++N + +A  L  E   G    G IP
Sbjct: 103 GMGSNLNLLGEIECDASIMDGKSLNFGAVGALSGIKNPVSVANRLLCEGQKGKLSAGRIP 162

Query: 133 PIFLVGEGACTWAKSKGIWLPETTAEANEWLVTERAKVQWERYKSMIQRAKEKKENSYVV 192
           P FLVGEGA  WA   GI     +   N  ++T R  +      +  +R K K E +  V
Sbjct: 163 PCFLVGEGAYRWAVDHGI----PSCPPN--IMTTRFSL------AAFKRNKRKLELAERV 222

Query: 193 NSCSSQDNTRISEVQSHNPLEGTTCQSATLNDAENDC-TMDTVGVICVDSEGHVASGASS 252
           ++   Q   R                    ++ END  T+DTVG + VD EG+VA+  SS
Sbjct: 223 DTDFMQLKKRRQS-----------------SEKENDSGTLDTVGAVVVDHEGNVAAAVSS 282

Query: 253 GGIAMKVSGRVGLAAMYGSGCWASSKGPFGTPFMVGGCVSGGGEYLMKGFAARECCVSLS 312
           GG+A+K  GRVG AA+YG GCWA + G    P+      SG GE+L++   AREC  +L 
Sbjct: 283 GGLALKHPGRVGQAALYGCGCWAENTGAH-NPYSTAVSTSGCGEHLVRTILARECSHALQ 342

Query: 313 LSQAGPASACMKVLRN-FVQESSQHNTD-KSAGILLVQADAPKLAPGNPQKLKA--VEIA 372
              A    A ++ ++N F+      + D    G++++++      P + Q  +   VE  
Sbjct: 343 AEDA--HQALLETMQNKFISSPFLASEDGVLGGVIVLRSCRCSAEPDSSQNKQTLLVEFL 395

Query: 373 AAYSSLSFGMGYFASSMERPKVSILR 394
            ++++ S  +GY ++   + K  I R
Sbjct: 403 WSHTTESMCVGYMSAQDGKAKTHISR 395

BLAST of CmaCh06G010960 vs. ExPASy Swiss-Prot
Match: Q8R1G1 (Threonine aspartase 1 OS=Mus musculus OX=10090 GN=Tasp1 PE=2 SV=1)

HSP 1 Score: 177.9 bits (450), Expect = 2.3e-43
Identity = 141/390 (36.15%), Postives = 198/390 (50.77%), Query Frame = 0

Query: 13  FVAVHVGAGFHAPSNEKALRSAMKRACLAASLVLRKGSGGCLDAVSAAIQVLEDDPVTNA 72
           FV VH GAG+H+ S  K  +   KRAC  A   L+ G+    DAV+AA+  LED P TNA
Sbjct: 43  FVLVHAGAGYHSESKAKEYKHVCKRACQKAIEKLQAGA-LATDAVAAALVELEDSPFTNA 102

Query: 73  GRGSNLTEDGRVECDASIMDGDTGAFGAVGAVPGVRNAIKIAALLAKEQILGSSLLGLIP 132
           G GSNL   G +ECDASIMDG +  FGAVGA+ G++N + +A  L  E   G    G IP
Sbjct: 103 GIGSNLNLLGEIECDASIMDGKSLNFGAVGALSGIKNPVSVAHRLLCEGQKGKLSAGRIP 162

Query: 133 PIFLVGEGACTWAKSKGI-WLPETTAEANEWLVTERAKVQWERYKSMIQRAKEKKENSYV 192
           P FLVGEGA  WA   GI   P +T      + T  +   ++R K  ++ A E+ E  ++
Sbjct: 163 PCFLVGEGAYRWAVDHGIPSCPPST------MTTRFSLAAFKRNKRKLELA-ERVETDFI 222

Query: 193 VNSCSSQDNTRISEVQSHNPLEGTTCQSATLNDAENDC-TMDTVGVICVDSEGHVASGAS 252
                 Q + +                       END  T+DTVG + VD EG+VA+  S
Sbjct: 223 QLKRRRQSSAK-----------------------ENDSGTLDTVGAVVVDHEGNVAAAVS 282

Query: 253 SGGIAMKVSGRVGLAAMYGSGCWASSKG---PFGTPFMVGGCVSGGGEYLMKGFAARECC 312
           SGG+A+K  GRVG AA+YG GCWA + G   P+ T     GC    GE+L++   AREC 
Sbjct: 283 SGGLALKHPGRVGQAALYGCGCWAENTGAQNPYSTAVSTSGC----GEHLVRTILARECS 342

Query: 313 VSLSLSQAGPASACMKVLRN-FVQESSQHNTDKSAGILLVQADAPKLAPGNPQKLK---A 372
            +L    A    A ++ ++N F+        D   G ++V       +  +  + K    
Sbjct: 343 HALQAEDA--HQALLETMQNKFISSPFLACEDGVLGGVIVLRSCRCSSESDSSQDKQTLL 395

Query: 373 VEIAAAYSSLSFGMGYFASSMERPKVSILR 394
           VE   ++++ S  +GY ++   + K  I R
Sbjct: 403 VEFLWSHTTESMCVGYMSAQDGKAKTHISR 395

BLAST of CmaCh06G010960 vs. ExPASy Swiss-Prot
Match: Q9P6N7 (Putative threonine aspartase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPAC823.09c PE=3 SV=3)

HSP 1 Score: 111.7 bits (278), Expect = 2.0e-23
Identity = 105/391 (26.85%), Postives = 169/391 (43.22%), Query Frame = 0

Query: 14  VAVHVGAGFHAPSNEKALRSAMKRACLAASLVLRKGSGGCLDAVSAAIQVLEDDPVTNAG 73
           V +H GAG ++   E   +     AC AA   L+ G      A+ AA +++ED PVTNAG
Sbjct: 6   VILHAGAGLYSGQREIQAKKTCSDACKAAIQALKVGQSALSAAIQAA-KIMEDSPVTNAG 65

Query: 74  RGSNLTEDGRVECDASIMDGDTGAFGAVGAVPGVRNAIKIAA-LLAKEQILGSSLLGLIP 133
            GSNL  DG+VEC+A +MD ++G   +V      R+  +    +L K +++  S  GL+P
Sbjct: 66  VGSNLNIDGKVECEAGVMDSESGLTASVACCNCCRHPSEACLYILNKRKVM--SQHGLVP 125

Query: 134 PIFLVGEGACTWAKSKGIWLPETTAEANEWLVTERAKVQWERYKSMIQRAKEKKENSYVV 193
           P  LVG G           + +    +N  LV E                      S+++
Sbjct: 126 PAMLVGNG-----------IEKLLLHSNIKLVPE----------------------SHLI 185

Query: 194 NSCSSQDNTRISEVQSHNPLEGTTCQSATLNDAENDCTMDTVGVICVDSEGHVASGASSG 253
              S +   +  E+   NP+              N  + DT+GVICVD  G +A  +SSG
Sbjct: 186 TERSMKTQIKWKEILYQNPI--------------NLSSQDTIGVICVDKNGRIAVVSSSG 245

Query: 254 GIAMKVSGRVGLAAMYGSGCWASSKGPFGTPFMVGGCVSGGGEYLMKG-FAARECCVSLS 313
           G+ +K +GR+G + + G G W  S              SG GE++    FA R     L 
Sbjct: 246 GLLLKPAGRIGSSPIPGHGFWIESFDNKSHSSTCAVATSGTGEHISNTCFACRSS--QLL 305

Query: 314 LSQAGPASACMKVLRNFVQE-SSQHNTDKSAGILLVQADAPKLAPGNPQKLKAVEIAAAY 373
           +S+    S+  K++ +F +  S+   +D   GI+  + +       +    K +    A+
Sbjct: 306 VSEDNVVSSLNKLINDFHEHPSATLYSDLQVGIIFAKVET------SNSHNKRIIFGLAH 338

Query: 374 SSLSFGMGYFASSMERPKVSILRSTKQNNKM 402
           SS     G+      +P   I R   + + +
Sbjct: 366 SSPDMVFGFMKGDHSKPTTEISRKGSKRSSV 338

BLAST of CmaCh06G010960 vs. ExPASy Swiss-Prot
Match: Q6GM78 (Isoaspartyl peptidase/L-asparaginase OS=Xenopus laevis OX=8355 GN=asrgl1 PE=2 SV=1)

HSP 1 Score: 107.1 bits (266), Expect = 5.0e-22
Identity = 100/292 (34.25%), Postives = 134/292 (45.89%), Query Frame = 0

Query: 14  VAVHVGAGFHAPSNEKALRSAMKRACLAASLVLRKGSGGCLDAVSAAIQVLEDDPVTNAG 73
           + VH GAG      +   R+ +KRA L    VL +G G  L AV  A+ VLED+ + NAG
Sbjct: 5   IVVHGGAGKIVEELDATYRAGVKRAVLKGYDVLSQG-GSALTAVEEAVIVLEDEQIFNAG 64

Query: 74  RGSNLTEDGRVECDASIMDGDTGAFGAVGAVPGVRNAIKIAALLAKEQILGSSLLGLIPP 133
            GS L E G +E DA IMDG     GAV A+  + N IK+A L+ ++             
Sbjct: 65  HGSVLNEKGDIEMDAIIMDGKNLDSGAVSAIRNIANPIKLARLVMEK----------TDH 124

Query: 134 IFLVGEGACTWAKSKGIWLPETTAEANEWLVTERAKVQWERYKSMIQRAKEKKENSYVVN 193
           + L  EGA  +AK++GI  PE     NE LVTER++ +W          K  KENS    
Sbjct: 125 MLLTCEGATLFAKAQGI--PEV---PNESLVTERSRKRW---------MKNLKENS---- 184

Query: 194 SCSSQDNTRISEVQSHNPLEGTTCQSATLNDAENDCTMDTVGVICVDSEGHVASGASSGG 253
                                        N   +   + TVG + +D EG+VA   S+GG
Sbjct: 185 -----------------------------NPVADQIGLGTVGAVAIDCEGNVACATSTGG 230

Query: 254 IAMKVSGRVGLAAMYGSGCWASSKGPFGTPFMVGG-CVSGGGEYLMKGFAAR 305
           +  K+ GRVG  A  GSG +A +         VG    +G GE +MK   AR
Sbjct: 245 LTNKMVGRVGDTACIGSGGYADNN--------VGAVSTTGHGESIMKVILAR 230

BLAST of CmaCh06G010960 vs. ExPASy TrEMBL
Match: A0A6J1IIB2 (putative threonine aspartase OS=Cucurbita maxima OX=3661 GN=LOC111474081 PE=4 SV=1)

HSP 1 Score: 815.5 bits (2105), Expect = 1.1e-232
Identity = 418/418 (100.00%), Postives = 418/418 (100.00%), Query Frame = 0

Query: 1   MAAEAQDQGHRFFVAVHVGAGFHAPSNEKALRSAMKRACLAASLVLRKGSGGCLDAVSAA 60
           MAAEAQDQGHRFFVAVHVGAGFHAPSNEKALRSAMKRACLAASLVLRKGSGGCLDAVSAA
Sbjct: 1   MAAEAQDQGHRFFVAVHVGAGFHAPSNEKALRSAMKRACLAASLVLRKGSGGCLDAVSAA 60

Query: 61  IQVLEDDPVTNAGRGSNLTEDGRVECDASIMDGDTGAFGAVGAVPGVRNAIKIAALLAKE 120
           IQVLEDDPVTNAGRGSNLTEDGRVECDASIMDGDTGAFGAVGAVPGVRNAIKIAALLAKE
Sbjct: 61  IQVLEDDPVTNAGRGSNLTEDGRVECDASIMDGDTGAFGAVGAVPGVRNAIKIAALLAKE 120

Query: 121 QILGSSLLGLIPPIFLVGEGACTWAKSKGIWLPETTAEANEWLVTERAKVQWERYKSMIQ 180
           QILGSSLLGLIPPIFLVGEGACTWAKSKGIWLPETTAEANEWLVTERAKVQWERYKSMIQ
Sbjct: 121 QILGSSLLGLIPPIFLVGEGACTWAKSKGIWLPETTAEANEWLVTERAKVQWERYKSMIQ 180

Query: 181 RAKEKKENSYVVNSCSSQDNTRISEVQSHNPLEGTTCQSATLNDAENDCTMDTVGVICVD 240
           RAKEKKENSYVVNSCSSQDNTRISEVQSHNPLEGTTCQSATLNDAENDCTMDTVGVICVD
Sbjct: 181 RAKEKKENSYVVNSCSSQDNTRISEVQSHNPLEGTTCQSATLNDAENDCTMDTVGVICVD 240

Query: 241 SEGHVASGASSGGIAMKVSGRVGLAAMYGSGCWASSKGPFGTPFMVGGCVSGGGEYLMKG 300
           SEGHVASGASSGGIAMKVSGRVGLAAMYGSGCWASSKGPFGTPFMVGGCVSGGGEYLMKG
Sbjct: 241 SEGHVASGASSGGIAMKVSGRVGLAAMYGSGCWASSKGPFGTPFMVGGCVSGGGEYLMKG 300

Query: 301 FAARECCVSLSLSQAGPASACMKVLRNFVQESSQHNTDKSAGILLVQADAPKLAPGNPQK 360
           FAARECCVSLSLSQAGPASACMKVLRNFVQESSQHNTDKSAGILLVQADAPKLAPGNPQK
Sbjct: 301 FAARECCVSLSLSQAGPASACMKVLRNFVQESSQHNTDKSAGILLVQADAPKLAPGNPQK 360

Query: 361 LKAVEIAAAYSSLSFGMGYFASSMERPKVSILRSTKQNNKMGIDHFEACIDLSDNGKL 419
           LKAVEIAAAYSSLSFGMGYFASSMERPKVSILRSTKQNNKMGIDHFEACIDLSDNGKL
Sbjct: 361 LKAVEIAAAYSSLSFGMGYFASSMERPKVSILRSTKQNNKMGIDHFEACIDLSDNGKL 418

BLAST of CmaCh06G010960 vs. ExPASy TrEMBL
Match: A0A6J1F0Q0 (putative threonine aspartase OS=Cucurbita moschata OX=3662 GN=LOC111441303 PE=4 SV=1)

HSP 1 Score: 808.5 bits (2087), Expect = 1.3e-230
Identity = 415/418 (99.28%), Postives = 417/418 (99.76%), Query Frame = 0

Query: 1   MAAEAQDQGHRFFVAVHVGAGFHAPSNEKALRSAMKRACLAASLVLRKGSGGCLDAVSAA 60
           MAAEAQDQGHRFFVAVHVGAGFHAPSNEKALRSAMKRACLAASLVLRKGSGGCLDAVSAA
Sbjct: 1   MAAEAQDQGHRFFVAVHVGAGFHAPSNEKALRSAMKRACLAASLVLRKGSGGCLDAVSAA 60

Query: 61  IQVLEDDPVTNAGRGSNLTEDGRVECDASIMDGDTGAFGAVGAVPGVRNAIKIAALLAKE 120
           IQVLEDDPVTNAGRGSNLTEDGRVECDASIMDGDTGAFGAVGAVPGVRNAIKIAALLAKE
Sbjct: 61  IQVLEDDPVTNAGRGSNLTEDGRVECDASIMDGDTGAFGAVGAVPGVRNAIKIAALLAKE 120

Query: 121 QILGSSLLGLIPPIFLVGEGACTWAKSKGIWLPETTAEANEWLVTERAKVQWERYKSMIQ 180
           QILGSSLLGLIPPIFLVGEGACTWAKSKGIWLPETTAEANEWLVTERAKVQWERYKSMIQ
Sbjct: 121 QILGSSLLGLIPPIFLVGEGACTWAKSKGIWLPETTAEANEWLVTERAKVQWERYKSMIQ 180

Query: 181 RAKEKKENSYVVNSCSSQDNTRISEVQSHNPLEGTTCQSATLNDAENDCTMDTVGVICVD 240
           RAKEKKENSYVVNSCSSQDNTRISEVQSHNPLEGTTCQSATLNDAENDCTMDTVGVICVD
Sbjct: 181 RAKEKKENSYVVNSCSSQDNTRISEVQSHNPLEGTTCQSATLNDAENDCTMDTVGVICVD 240

Query: 241 SEGHVASGASSGGIAMKVSGRVGLAAMYGSGCWASSKGPFGTPFMVGGCVSGGGEYLMKG 300
           SEGH+ASGASSGGIAMKVSGRVGLAAMYGSGCWASSKGPFGTPFMVGGCVSGGGEYLMKG
Sbjct: 241 SEGHIASGASSGGIAMKVSGRVGLAAMYGSGCWASSKGPFGTPFMVGGCVSGGGEYLMKG 300

Query: 301 FAARECCVSLSLSQAGPASACMKVLRNFVQESSQHNTDKSAGILLVQADAPKLAPGNPQK 360
           FAARECCVSLSLSQAGPASACMKVLRNFVQESSQHNTDKSAGILLVQADAPKLAPG+PQK
Sbjct: 301 FAARECCVSLSLSQAGPASACMKVLRNFVQESSQHNTDKSAGILLVQADAPKLAPGHPQK 360

Query: 361 LKAVEIAAAYSSLSFGMGYFASSMERPKVSILRSTKQNNKMGIDHFEACIDLSDNGKL 419
           LKAVEIAAAYSSLSFGMGYFASSMERPKVSILRSTKQNNKMGIDHFEA IDLSDNGKL
Sbjct: 361 LKAVEIAAAYSSLSFGMGYFASSMERPKVSILRSTKQNNKMGIDHFEARIDLSDNGKL 418

BLAST of CmaCh06G010960 vs. ExPASy TrEMBL
Match: A0A6J1D2M3 (putative threonine aspartase OS=Momordica charantia OX=3673 GN=LOC111016424 PE=4 SV=1)

HSP 1 Score: 742.7 bits (1916), Expect = 8.7e-211
Identity = 384/418 (91.87%), Postives = 401/418 (95.93%), Query Frame = 0

Query: 1   MAAEAQDQGHRFFVAVHVGAGFHAPSNEKALRSAMKRACLAASLVLRKGSGGCLDAVSAA 60
           MA EAQDQGHRFFVAVHVGAGFHAPSNEKALRSAMKRACLAA++VLR+GSGGCLDAVSAA
Sbjct: 1   MAGEAQDQGHRFFVAVHVGAGFHAPSNEKALRSAMKRACLAAAVVLRQGSGGCLDAVSAA 60

Query: 61  IQVLEDDPVTNAGRGSNLTEDGRVECDASIMDGDTGAFGAVGAVPGVRNAIKIAALLAKE 120
           IQVLEDDPVTNAGRGSNLTEDG VECDASIMDGDTGAFGAVGAVPGV+NAI+IAALLAKE
Sbjct: 61  IQVLEDDPVTNAGRGSNLTEDGHVECDASIMDGDTGAFGAVGAVPGVQNAIQIAALLAKE 120

Query: 121 QILGSSLLGLIPPIFLVGEGACTWAKSKGIWLPETTAEANEWLVTERAKVQWERYKSMIQ 180
           Q+LGSSLLGLIPPIFLVGEGA TWAKSKGI LPETTAEA+EWLVTERAKVQW+RYKSMI+
Sbjct: 121 QMLGSSLLGLIPPIFLVGEGARTWAKSKGICLPETTAEADEWLVTERAKVQWKRYKSMIE 180

Query: 181 RAKEKKENSYVVNSCSSQDNTRISEVQSHNPLEGTTCQSATLNDAENDCTMDTVGVICVD 240
           RAKE+K+ S V +SCS QDN RISEVQSHNPLEGTTCQS+TL DAE DCTMDTVGVICVD
Sbjct: 181 RAKEEKKISNVTHSCSPQDNARISEVQSHNPLEGTTCQSSTLTDAEKDCTMDTVGVICVD 240

Query: 241 SEGHVASGASSGGIAMKVSGRVGLAAMYGSGCWASSKGPFGTPFMVGGCVSGGGEYLMKG 300
           +EGHVASGASSGGIAMKVSGRVGLAAMYGSGCWASSKGPFG PFMVGGCVSGGGEYLMKG
Sbjct: 241 NEGHVASGASSGGIAMKVSGRVGLAAMYGSGCWASSKGPFGAPFMVGGCVSGGGEYLMKG 300

Query: 301 FAARECCVSLSLSQAGPASACMKVLRNFVQESSQHNTDKSAGILLVQADAPKLAPGNPQK 360
           FAARECCVSLSLSQAGPASACMKVLRNFVQESS+ NTDKSAGILLVQAD  K APGNPQK
Sbjct: 301 FAARECCVSLSLSQAGPASACMKVLRNFVQESSRFNTDKSAGILLVQADIHKSAPGNPQK 360

Query: 361 LKAVEIAAAYSSLSFGMGYFASSMERPKVSILRSTKQNNKMGIDHFEACIDLSDNGKL 419
           LKAVEIAAAYSSLSFG+GYFASSMERPKVSILRSTKQN+KMGIDHFEA IDLSDNGKL
Sbjct: 361 LKAVEIAAAYSSLSFGVGYFASSMERPKVSILRSTKQNDKMGIDHFEARIDLSDNGKL 418

BLAST of CmaCh06G010960 vs. ExPASy TrEMBL
Match: A0A0A0L7I2 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G122610 PE=4 SV=1)

HSP 1 Score: 733.0 bits (1891), Expect = 6.9e-208
Identity = 376/418 (89.95%), Postives = 397/418 (94.98%), Query Frame = 0

Query: 1   MAAEAQDQGHRFFVAVHVGAGFHAPSNEKALRSAMKRACLAASLVLRKGSGGCLDAVSAA 60
           MAAEAQD+ HRFFVAVHVGAGFHAPSNEKALRSAMKRACLAA++VLRKGSGGCLDAVSAA
Sbjct: 54  MAAEAQDEAHRFFVAVHVGAGFHAPSNEKALRSAMKRACLAAAVVLRKGSGGCLDAVSAA 113

Query: 61  IQVLEDDPVTNAGRGSNLTEDGRVECDASIMDGDTGAFGAVGAVPGVRNAIKIAALLAKE 120
           I+VLEDDP TNAGRGSNLT+DG+VECDASIMDGDTGAFGAVGAVPGVRNAI+IA LLAKE
Sbjct: 114 IKVLEDDPATNAGRGSNLTDDGQVECDASIMDGDTGAFGAVGAVPGVRNAIQIATLLAKE 173

Query: 121 QILGSSLLGLIPPIFLVGEGACTWAKSKGIWLPETTAEANEWLVTERAKVQWERYKSMIQ 180
           QILGSSLLGLIPPIFLVGEGACTWAK+KGI LPET AEANEWLVTERAK QW+RYKSM++
Sbjct: 174 QILGSSLLGLIPPIFLVGEGACTWAKTKGICLPETIAEANEWLVTERAKAQWKRYKSMVE 233

Query: 181 RAKEKKENSYVVNSCSSQDNTRISEVQSHNPLEGTTCQSATLNDAENDCTMDTVGVICVD 240
           RAK++K  + VV+S S QDN RISE+QSHNP EGTTCQS+T ++ E DCTMDTVGVICVD
Sbjct: 234 RAKKEKGTTNVVHSSSPQDNARISELQSHNPPEGTTCQSSTRSNEEEDCTMDTVGVICVD 293

Query: 241 SEGHVASGASSGGIAMKVSGRVGLAAMYGSGCWASSKGPFGTPFMVGGCVSGGGEYLMKG 300
           SEGHVASGASSGGIAMKVSGRVGLAAMYGSGCWASSKGPFG PFMVGGCVSGGGEYLMKG
Sbjct: 294 SEGHVASGASSGGIAMKVSGRVGLAAMYGSGCWASSKGPFGAPFMVGGCVSGGGEYLMKG 353

Query: 301 FAARECCVSLSLSQAGPASACMKVLRNFVQESSQHNTDKSAGILLVQADAPKLAPGNPQK 360
           FAARECCVSLSLSQAGPASACMKVLRNFVQESSQHN DKSAGILLVQADA KLAPGN QK
Sbjct: 354 FAARECCVSLSLSQAGPASACMKVLRNFVQESSQHNADKSAGILLVQADASKLAPGNQQK 413

Query: 361 LKAVEIAAAYSSLSFGMGYFASSMERPKVSILRSTKQNNKMGIDHFEACIDLSDNGKL 419
           LKAVEIAAAYSSLSFGMGYFASSM+RPKVSILRSTKQNNK+GIDHFEA IDLSDNGKL
Sbjct: 414 LKAVEIAAAYSSLSFGMGYFASSMDRPKVSILRSTKQNNKLGIDHFEARIDLSDNGKL 471

BLAST of CmaCh06G010960 vs. ExPASy TrEMBL
Match: A0A5D3D030 (Putative threonine aspartase isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold434G004510 PE=4 SV=1)

HSP 1 Score: 726.1 bits (1873), Expect = 8.4e-206
Identity = 377/421 (89.55%), Postives = 395/421 (93.82%), Query Frame = 0

Query: 1   MAAEAQDQGHRFFVAVHVGAGFHAPSNEKALRSAMKRACLAASLVLRKGSGGCLDAVSAA 60
           MAAEAQ++ HRFFVAVHVGAGFHAPSNEKALRSAMKRACLAA++VLRKGSGGCLDAVSAA
Sbjct: 1   MAAEAQEEAHRFFVAVHVGAGFHAPSNEKALRSAMKRACLAAAVVLRKGSGGCLDAVSAA 60

Query: 61  IQVLEDDPVTNAGRGSNLTEDGRVECDASIMDGDTGAFGAVGAVPGVRNAIKIAALLAKE 120
           I+VLEDDP TNAGRGSNLTEDG+VECDASIMDGDTGAFGAVGAVPGVRNAI+IAALLAKE
Sbjct: 61  IKVLEDDPATNAGRGSNLTEDGQVECDASIMDGDTGAFGAVGAVPGVRNAIQIAALLAKE 120

Query: 121 QILGSSLLGLIPPIFLVGEGACTWAKSKGIWLPETTAEANEWLVTERAKVQWERYKSMIQ 180
           QILGSSLLGLIPPIFLVGEGA TWAK+KGI LPET AEANEWLVTERAKVQW+RYKSM++
Sbjct: 121 QILGSSLLGLIPPIFLVGEGARTWAKTKGICLPETIAEANEWLVTERAKVQWKRYKSMVE 180

Query: 181 RAKEKKENSYVVNSCSSQDNTRISEVQSHNPLEGTTCQSATLN---DAENDCTMDTVGVI 240
           RAK++K  S V +SC  QDN RISEVQSHNP EGTTCQS+T +   + E DCTMDTVGVI
Sbjct: 181 RAKKEKGTSNVAHSCCPQDNARISEVQSHNPPEGTTCQSSTRSNEEEEEEDCTMDTVGVI 240

Query: 241 CVDSEGHVASGASSGGIAMKVSGRVGLAAMYGSGCWASSKGPFGTPFMVGGCVSGGGEYL 300
           CVDSEGHVASGASSGGIAMKVSGRVGLAAMYGSGCWASSKGPFG PFMVGGCVSGGGEYL
Sbjct: 241 CVDSEGHVASGASSGGIAMKVSGRVGLAAMYGSGCWASSKGPFGAPFMVGGCVSGGGEYL 300

Query: 301 MKGFAARECCVSLSLSQAGPASACMKVLRNFVQESSQHNTDKSAGILLVQADAPKLAPGN 360
           MKGFAARECCVSLSLSQAGP SACMKVLRNFVQESSQHN DKSAGILLVQADA K APGN
Sbjct: 301 MKGFAARECCVSLSLSQAGPGSACMKVLRNFVQESSQHNADKSAGILLVQADASKSAPGN 360

Query: 361 PQKLKAVEIAAAYSSLSFGMGYFASSMERPKVSILRSTKQNNKMGIDHFEACIDLSDNGK 419
           PQKLKAVEIAAAYSSLSFGMGYFASSM+RPKVSILRSTKQNNKMGIDHFEA IDLSDN K
Sbjct: 361 PQKLKAVEIAAAYSSLSFGMGYFASSMDRPKVSILRSTKQNNKMGIDHFEARIDLSDNRK 420

BLAST of CmaCh06G010960 vs. NCBI nr
Match: XP_022975108.1 (putative threonine aspartase [Cucurbita maxima])

HSP 1 Score: 815.5 bits (2105), Expect = 2.2e-232
Identity = 418/418 (100.00%), Postives = 418/418 (100.00%), Query Frame = 0

Query: 1   MAAEAQDQGHRFFVAVHVGAGFHAPSNEKALRSAMKRACLAASLVLRKGSGGCLDAVSAA 60
           MAAEAQDQGHRFFVAVHVGAGFHAPSNEKALRSAMKRACLAASLVLRKGSGGCLDAVSAA
Sbjct: 1   MAAEAQDQGHRFFVAVHVGAGFHAPSNEKALRSAMKRACLAASLVLRKGSGGCLDAVSAA 60

Query: 61  IQVLEDDPVTNAGRGSNLTEDGRVECDASIMDGDTGAFGAVGAVPGVRNAIKIAALLAKE 120
           IQVLEDDPVTNAGRGSNLTEDGRVECDASIMDGDTGAFGAVGAVPGVRNAIKIAALLAKE
Sbjct: 61  IQVLEDDPVTNAGRGSNLTEDGRVECDASIMDGDTGAFGAVGAVPGVRNAIKIAALLAKE 120

Query: 121 QILGSSLLGLIPPIFLVGEGACTWAKSKGIWLPETTAEANEWLVTERAKVQWERYKSMIQ 180
           QILGSSLLGLIPPIFLVGEGACTWAKSKGIWLPETTAEANEWLVTERAKVQWERYKSMIQ
Sbjct: 121 QILGSSLLGLIPPIFLVGEGACTWAKSKGIWLPETTAEANEWLVTERAKVQWERYKSMIQ 180

Query: 181 RAKEKKENSYVVNSCSSQDNTRISEVQSHNPLEGTTCQSATLNDAENDCTMDTVGVICVD 240
           RAKEKKENSYVVNSCSSQDNTRISEVQSHNPLEGTTCQSATLNDAENDCTMDTVGVICVD
Sbjct: 181 RAKEKKENSYVVNSCSSQDNTRISEVQSHNPLEGTTCQSATLNDAENDCTMDTVGVICVD 240

Query: 241 SEGHVASGASSGGIAMKVSGRVGLAAMYGSGCWASSKGPFGTPFMVGGCVSGGGEYLMKG 300
           SEGHVASGASSGGIAMKVSGRVGLAAMYGSGCWASSKGPFGTPFMVGGCVSGGGEYLMKG
Sbjct: 241 SEGHVASGASSGGIAMKVSGRVGLAAMYGSGCWASSKGPFGTPFMVGGCVSGGGEYLMKG 300

Query: 301 FAARECCVSLSLSQAGPASACMKVLRNFVQESSQHNTDKSAGILLVQADAPKLAPGNPQK 360
           FAARECCVSLSLSQAGPASACMKVLRNFVQESSQHNTDKSAGILLVQADAPKLAPGNPQK
Sbjct: 301 FAARECCVSLSLSQAGPASACMKVLRNFVQESSQHNTDKSAGILLVQADAPKLAPGNPQK 360

Query: 361 LKAVEIAAAYSSLSFGMGYFASSMERPKVSILRSTKQNNKMGIDHFEACIDLSDNGKL 419
           LKAVEIAAAYSSLSFGMGYFASSMERPKVSILRSTKQNNKMGIDHFEACIDLSDNGKL
Sbjct: 361 LKAVEIAAAYSSLSFGMGYFASSMERPKVSILRSTKQNNKMGIDHFEACIDLSDNGKL 418

BLAST of CmaCh06G010960 vs. NCBI nr
Match: XP_022934036.1 (putative threonine aspartase [Cucurbita moschata] >KAG6597152.1 putative threonine aspartase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 808.5 bits (2087), Expect = 2.7e-230
Identity = 415/418 (99.28%), Postives = 417/418 (99.76%), Query Frame = 0

Query: 1   MAAEAQDQGHRFFVAVHVGAGFHAPSNEKALRSAMKRACLAASLVLRKGSGGCLDAVSAA 60
           MAAEAQDQGHRFFVAVHVGAGFHAPSNEKALRSAMKRACLAASLVLRKGSGGCLDAVSAA
Sbjct: 1   MAAEAQDQGHRFFVAVHVGAGFHAPSNEKALRSAMKRACLAASLVLRKGSGGCLDAVSAA 60

Query: 61  IQVLEDDPVTNAGRGSNLTEDGRVECDASIMDGDTGAFGAVGAVPGVRNAIKIAALLAKE 120
           IQVLEDDPVTNAGRGSNLTEDGRVECDASIMDGDTGAFGAVGAVPGVRNAIKIAALLAKE
Sbjct: 61  IQVLEDDPVTNAGRGSNLTEDGRVECDASIMDGDTGAFGAVGAVPGVRNAIKIAALLAKE 120

Query: 121 QILGSSLLGLIPPIFLVGEGACTWAKSKGIWLPETTAEANEWLVTERAKVQWERYKSMIQ 180
           QILGSSLLGLIPPIFLVGEGACTWAKSKGIWLPETTAEANEWLVTERAKVQWERYKSMIQ
Sbjct: 121 QILGSSLLGLIPPIFLVGEGACTWAKSKGIWLPETTAEANEWLVTERAKVQWERYKSMIQ 180

Query: 181 RAKEKKENSYVVNSCSSQDNTRISEVQSHNPLEGTTCQSATLNDAENDCTMDTVGVICVD 240
           RAKEKKENSYVVNSCSSQDNTRISEVQSHNPLEGTTCQSATLNDAENDCTMDTVGVICVD
Sbjct: 181 RAKEKKENSYVVNSCSSQDNTRISEVQSHNPLEGTTCQSATLNDAENDCTMDTVGVICVD 240

Query: 241 SEGHVASGASSGGIAMKVSGRVGLAAMYGSGCWASSKGPFGTPFMVGGCVSGGGEYLMKG 300
           SEGH+ASGASSGGIAMKVSGRVGLAAMYGSGCWASSKGPFGTPFMVGGCVSGGGEYLMKG
Sbjct: 241 SEGHIASGASSGGIAMKVSGRVGLAAMYGSGCWASSKGPFGTPFMVGGCVSGGGEYLMKG 300

Query: 301 FAARECCVSLSLSQAGPASACMKVLRNFVQESSQHNTDKSAGILLVQADAPKLAPGNPQK 360
           FAARECCVSLSLSQAGPASACMKVLRNFVQESSQHNTDKSAGILLVQADAPKLAPG+PQK
Sbjct: 301 FAARECCVSLSLSQAGPASACMKVLRNFVQESSQHNTDKSAGILLVQADAPKLAPGHPQK 360

Query: 361 LKAVEIAAAYSSLSFGMGYFASSMERPKVSILRSTKQNNKMGIDHFEACIDLSDNGKL 419
           LKAVEIAAAYSSLSFGMGYFASSMERPKVSILRSTKQNNKMGIDHFEA IDLSDNGKL
Sbjct: 361 LKAVEIAAAYSSLSFGMGYFASSMERPKVSILRSTKQNNKMGIDHFEARIDLSDNGKL 418

BLAST of CmaCh06G010960 vs. NCBI nr
Match: XP_023541014.1 (putative threonine aspartase [Cucurbita pepo subsp. pepo])

HSP 1 Score: 806.6 bits (2082), Expect = 1.0e-229
Identity = 415/418 (99.28%), Postives = 416/418 (99.52%), Query Frame = 0

Query: 1   MAAEAQDQGHRFFVAVHVGAGFHAPSNEKALRSAMKRACLAASLVLRKGSGGCLDAVSAA 60
           MAAEAQDQGHRFFVAVHVGAGFHAPSNEKALRSAMKRACLAASLVLRKGSGGCLDAVSAA
Sbjct: 1   MAAEAQDQGHRFFVAVHVGAGFHAPSNEKALRSAMKRACLAASLVLRKGSGGCLDAVSAA 60

Query: 61  IQVLEDDPVTNAGRGSNLTEDGRVECDASIMDGDTGAFGAVGAVPGVRNAIKIAALLAKE 120
           IQVLEDDPVTNAGRGSNLTEDGRVECDASIMDGDTGAFGAVGAVPGVRNAIKIAALLAKE
Sbjct: 61  IQVLEDDPVTNAGRGSNLTEDGRVECDASIMDGDTGAFGAVGAVPGVRNAIKIAALLAKE 120

Query: 121 QILGSSLLGLIPPIFLVGEGACTWAKSKGIWLPETTAEANEWLVTERAKVQWERYKSMIQ 180
           QILGSSLLGLIPPIFLVGEGACTWAKSKGIWLPETTAEANEWLVTERAKVQWERYKSMIQ
Sbjct: 121 QILGSSLLGLIPPIFLVGEGACTWAKSKGIWLPETTAEANEWLVTERAKVQWERYKSMIQ 180

Query: 181 RAKEKKENSYVVNSCSSQDNTRISEVQSHNPLEGTTCQSATLNDAENDCTMDTVGVICVD 240
           RAKEKKENSYVVNSCSSQDNTRISEVQS NPLEGTTCQSATLNDAENDCTMDTVGVICVD
Sbjct: 181 RAKEKKENSYVVNSCSSQDNTRISEVQSPNPLEGTTCQSATLNDAENDCTMDTVGVICVD 240

Query: 241 SEGHVASGASSGGIAMKVSGRVGLAAMYGSGCWASSKGPFGTPFMVGGCVSGGGEYLMKG 300
           SEGH+ASGASSGGIAMKVSGRVGLAAMYGSGCWASSKGPFGTPFMVGGCVSGGGEYLMKG
Sbjct: 241 SEGHIASGASSGGIAMKVSGRVGLAAMYGSGCWASSKGPFGTPFMVGGCVSGGGEYLMKG 300

Query: 301 FAARECCVSLSLSQAGPASACMKVLRNFVQESSQHNTDKSAGILLVQADAPKLAPGNPQK 360
           FAARECCVSLSLSQAGPASACMKVLRNFVQESSQHNTDKSAGILLVQADAPKLAPGNPQK
Sbjct: 301 FAARECCVSLSLSQAGPASACMKVLRNFVQESSQHNTDKSAGILLVQADAPKLAPGNPQK 360

Query: 361 LKAVEIAAAYSSLSFGMGYFASSMERPKVSILRSTKQNNKMGIDHFEACIDLSDNGKL 419
           LKAVEIAAAYSSLSFGMGYFASSMERPKVSILRSTKQNNKMGIDHFEA IDLSDNGKL
Sbjct: 361 LKAVEIAAAYSSLSFGMGYFASSMERPKVSILRSTKQNNKMGIDHFEARIDLSDNGKL 418

BLAST of CmaCh06G010960 vs. NCBI nr
Match: XP_022147521.1 (putative threonine aspartase [Momordica charantia])

HSP 1 Score: 742.7 bits (1916), Expect = 1.8e-210
Identity = 384/418 (91.87%), Postives = 401/418 (95.93%), Query Frame = 0

Query: 1   MAAEAQDQGHRFFVAVHVGAGFHAPSNEKALRSAMKRACLAASLVLRKGSGGCLDAVSAA 60
           MA EAQDQGHRFFVAVHVGAGFHAPSNEKALRSAMKRACLAA++VLR+GSGGCLDAVSAA
Sbjct: 1   MAGEAQDQGHRFFVAVHVGAGFHAPSNEKALRSAMKRACLAAAVVLRQGSGGCLDAVSAA 60

Query: 61  IQVLEDDPVTNAGRGSNLTEDGRVECDASIMDGDTGAFGAVGAVPGVRNAIKIAALLAKE 120
           IQVLEDDPVTNAGRGSNLTEDG VECDASIMDGDTGAFGAVGAVPGV+NAI+IAALLAKE
Sbjct: 61  IQVLEDDPVTNAGRGSNLTEDGHVECDASIMDGDTGAFGAVGAVPGVQNAIQIAALLAKE 120

Query: 121 QILGSSLLGLIPPIFLVGEGACTWAKSKGIWLPETTAEANEWLVTERAKVQWERYKSMIQ 180
           Q+LGSSLLGLIPPIFLVGEGA TWAKSKGI LPETTAEA+EWLVTERAKVQW+RYKSMI+
Sbjct: 121 QMLGSSLLGLIPPIFLVGEGARTWAKSKGICLPETTAEADEWLVTERAKVQWKRYKSMIE 180

Query: 181 RAKEKKENSYVVNSCSSQDNTRISEVQSHNPLEGTTCQSATLNDAENDCTMDTVGVICVD 240
           RAKE+K+ S V +SCS QDN RISEVQSHNPLEGTTCQS+TL DAE DCTMDTVGVICVD
Sbjct: 181 RAKEEKKISNVTHSCSPQDNARISEVQSHNPLEGTTCQSSTLTDAEKDCTMDTVGVICVD 240

Query: 241 SEGHVASGASSGGIAMKVSGRVGLAAMYGSGCWASSKGPFGTPFMVGGCVSGGGEYLMKG 300
           +EGHVASGASSGGIAMKVSGRVGLAAMYGSGCWASSKGPFG PFMVGGCVSGGGEYLMKG
Sbjct: 241 NEGHVASGASSGGIAMKVSGRVGLAAMYGSGCWASSKGPFGAPFMVGGCVSGGGEYLMKG 300

Query: 301 FAARECCVSLSLSQAGPASACMKVLRNFVQESSQHNTDKSAGILLVQADAPKLAPGNPQK 360
           FAARECCVSLSLSQAGPASACMKVLRNFVQESS+ NTDKSAGILLVQAD  K APGNPQK
Sbjct: 301 FAARECCVSLSLSQAGPASACMKVLRNFVQESSRFNTDKSAGILLVQADIHKSAPGNPQK 360

Query: 361 LKAVEIAAAYSSLSFGMGYFASSMERPKVSILRSTKQNNKMGIDHFEACIDLSDNGKL 419
           LKAVEIAAAYSSLSFG+GYFASSMERPKVSILRSTKQN+KMGIDHFEA IDLSDNGKL
Sbjct: 361 LKAVEIAAAYSSLSFGVGYFASSMERPKVSILRSTKQNDKMGIDHFEARIDLSDNGKL 418

BLAST of CmaCh06G010960 vs. NCBI nr
Match: XP_038878205.1 (putative threonine aspartase [Benincasa hispida])

HSP 1 Score: 735.3 bits (1897), Expect = 2.9e-208
Identity = 379/417 (90.89%), Postives = 398/417 (95.44%), Query Frame = 0

Query: 1   MAAEAQDQGHRFFVAVHVGAGFHAPSNEKALRSAMKRACLAASLVLRKGSGGCLDAVSAA 60
           MAA+AQD+GHRFFVAVHVGAGFHAPSNEKALRSAMKRACLAA++VLRKGSGGCLDAVSAA
Sbjct: 1   MAAKAQDEGHRFFVAVHVGAGFHAPSNEKALRSAMKRACLAAAVVLRKGSGGCLDAVSAA 60

Query: 61  IQVLEDDPVTNAGRGSNLTEDGRVECDASIMDGDTGAFGAVGAVPGVRNAIKIAALLAKE 120
           IQVLEDDP TNAGRGSNLTEDG+VECDASIMDGDTGAFGAVGAVPGV+NAI+IAALLAKE
Sbjct: 61  IQVLEDDPATNAGRGSNLTEDGQVECDASIMDGDTGAFGAVGAVPGVQNAIQIAALLAKE 120

Query: 121 QILGSSLLGLIPPIFLVGEGACTWAKSKGIWLPETTAEANEWLVTERAKVQWERYKSMIQ 180
           QILGS L+GLIPPIFLVGEGA TWAKSKGI LPET AEANEWLVTERAK+QW+RYKSM++
Sbjct: 121 QILGSPLMGLIPPIFLVGEGARTWAKSKGICLPETIAEANEWLVTERAKLQWKRYKSMVE 180

Query: 181 RAKEKKENSYVVNSCSSQDNTRISEVQSHNPLEGTTCQSATLNDAENDCTMDTVGVICVD 240
           RAK++K  S V +SCS QD  RISEVQSHNPLEG TCQS+TL+D E DC MDTVGVICVD
Sbjct: 181 RAKKEKGTSNVTHSCSPQDIARISEVQSHNPLEGATCQSSTLSDDEKDCIMDTVGVICVD 240

Query: 241 SEGHVASGASSGGIAMKVSGRVGLAAMYGSGCWASSKGPFGTPFMVGGCVSGGGEYLMKG 300
           SEGHVASGASSGGIAMKVSGRVGLAAMYGSGCWASSKGPFG PFMVGGCVSGGGEYLMKG
Sbjct: 241 SEGHVASGASSGGIAMKVSGRVGLAAMYGSGCWASSKGPFGAPFMVGGCVSGGGEYLMKG 300

Query: 301 FAARECCVSLSLSQAGPASACMKVLRNFVQESSQHNTDKSAGILLVQADAPKLAPGNPQK 360
           FAARECCVSLSLSQAGPASACMKVLRNFVQESSQ NTDKSAGILLVQADAPKLAPGNPQK
Sbjct: 301 FAARECCVSLSLSQAGPASACMKVLRNFVQESSQLNTDKSAGILLVQADAPKLAPGNPQK 360

Query: 361 LKAVEIAAAYSSLSFGMGYFASSMERPKVSILRSTKQNNKMGIDHFEACIDLSDNGK 418
           LKAVEIAAAYSSLSFGMGYFASSM+RPKVSILRSTKQN+KMGIDHFEA IDLSDNGK
Sbjct: 361 LKAVEIAAAYSSLSFGMGYFASSMDRPKVSILRSTKQNSKMGIDHFEARIDLSDNGK 417

BLAST of CmaCh06G010960 vs. TAIR 10
Match: AT4G00590.1 (N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein )

HSP 1 Score: 514.6 bits (1324), Expect = 7.4e-146
Identity = 275/416 (66.11%), Postives = 330/416 (79.33%), Query Frame = 0

Query: 1   MAAEAQDQGHRFFVAVHVGAGFHAPSNEKALRSAMKRACLAASLVLRKGSGGCLDAVSAA 60
           MA +  DQ  RFFVAVHVGAG+HA +NEKALRS M+RACLAAS +LR+ SG C+DAVSAA
Sbjct: 2   MAGDGADQSRRFFVAVHVGAGYHAAANEKALRSVMRRACLAASTILRQDSGECIDAVSAA 61

Query: 61  IQVLEDDPVTNAGRGSNLTEDGRVECDASIMDGDTGAFGAVGAVPGVRNAIKIAALLAKE 120
           IQVLEDDP TNAGRGSNLTEDG VECDAS+MDGD+G FG VGAVPGVRNAIKIAALL KE
Sbjct: 62  IQVLEDDPSTNAGRGSNLTEDGHVECDASLMDGDSGMFGGVGAVPGVRNAIKIAALLVKE 121

Query: 121 QILGSSLLGLIPPIFLVGEGACTWAKSKGIWLPETTAEANEWLVTERAKVQWERYKSMIQ 180
           QI GS+LLG IPP+ LVGEGA  W KSK + +P T  EA++WLVTERA+ QW R+K+M+ 
Sbjct: 122 QITGSTLLGRIPPMLLVGEGARRWGKSKSVLIPGTVTEADQWLVTERARNQWRRFKAMLS 181

Query: 181 RAKEKKENSYVVNSCSSQDNTRISEVQSHNPLEGTTCQS--ATLNDAENDCTMDTVGVIC 240
               K       +  S++++ R +E          TC+   ++   A+ D  MDTVGVIC
Sbjct: 182 EVGAK-------SILSAEEHPRGTE-------NNETCEENVSSCAAADEDKIMDTVGVIC 241

Query: 241 VDSEGHVASGASSGGIAMKVSGRVGLAAMYGSGCWASSKGPFGTPFMVGGCVSGGGEYLM 300
           VD+EGH+A G+SSGGIAMK+SGRVGLAA YGSGCWASSKGPFG PF+VG CVSG GEYLM
Sbjct: 242 VDNEGHIACGSSSGGIAMKISGRVGLAATYGSGCWASSKGPFGAPFLVGCCVSGAGEYLM 301

Query: 301 KGFAARECCVSLSLSQAGPASACMKVLRNFV-QESSQHNT-DKSAGILLVQADAPKLAPG 360
           +GFAARECC SL+LSQAGPASA MKVLR+ + QESS+  T DK+AGIL+VQADA  + PG
Sbjct: 302 RGFAARECCTSLALSQAGPASAAMKVLRSVMHQESSKIGTADKTAGILVVQADASVVVPG 361

Query: 361 NPQKLKAVEIAAAYSSLSFGMGYFASSMERPKVSILRSTKQNNKMGIDHFEACIDL 413
           +  +L AVEIAAAYSSLSFG+GY+ +S+E+PK+SILR+ +Q ++ G+DHFEA IDL
Sbjct: 362 SKPELNAVEIAAAYSSLSFGVGYYGNSIEKPKISILRTRRQMSEAGVDHFEARIDL 403

BLAST of CmaCh06G010960 vs. TAIR 10
Match: AT4G00590.2 (N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein )

HSP 1 Score: 461.1 bits (1185), Expect = 9.7e-130
Identity = 254/416 (61.06%), Postives = 313/416 (75.24%), Query Frame = 0

Query: 1   MAAEAQDQGHRFFVAVHVGAGFHAPSNEKALRSAMKRACLAASLVLRKGSGGCLDAVSAA 60
           MA +  DQ  RFFVAVHVGAG+HA +NEKALRS M+RACLAAS +LR+ SG C+DAVSAA
Sbjct: 2   MAGDGADQSRRFFVAVHVGAGYHAAANEKALRSVMRRACLAASTILRQDSGECIDAVSAA 61

Query: 61  IQVLEDDPVTNAGRGSNLTEDGRVECDASIMDGDTGAFGAVGAVPGVRNAIKIAALLAKE 120
           IQVLEDDP TNAGRGSNLTEDG VECDAS+MDGD+G FG VGAVP      +++ +L+K 
Sbjct: 62  IQVLEDDPSTNAGRGSNLTEDGHVECDASLMDGDSGMFGGVGAVPD-----QVSEMLSK- 121

Query: 121 QILGSSLLGLIPPIFLVGEGACTWAKSKGIWLPETTAEANEWLVTERAKVQWERYKSMIQ 180
                    L+    LVGEGA  W KSK + +P T  EA++WLVTERA+ QW R+K+M+ 
Sbjct: 122 ---------LLLCWLLVGEGARRWGKSKSVLIPGTVTEADQWLVTERARNQWRRFKAMLS 181

Query: 181 RAKEKKENSYVVNSCSSQDNTRISEVQSHNPLEGTTCQS--ATLNDAENDCTMDTVGVIC 240
               K       +  S++++ R +E          TC+   ++   A+ D  MDTVGVIC
Sbjct: 182 EVGAK-------SILSAEEHPRGTE-------NNETCEENVSSCAAADEDKIMDTVGVIC 241

Query: 241 VDSEGHVASGASSGGIAMKVSGRVGLAAMYGSGCWASSKGPFGTPFMVGGCVSGGGEYLM 300
           VD+EGH+A G+SSGGIAMK+SGRVGLAA YGSGCWASSKGPFG PF+VG CVSG GEYLM
Sbjct: 242 VDNEGHIACGSSSGGIAMKISGRVGLAATYGSGCWASSKGPFGAPFLVGCCVSGAGEYLM 301

Query: 301 KGFAARECCVSLSLSQAGPASACMKVLRNFV-QESSQHNT-DKSAGILLVQADAPKLAPG 360
           +GFAARECC SL+LSQAGPASA MKVLR+ + QESS+  T DK+AGIL+VQADA  + PG
Sbjct: 302 RGFAARECCTSLALSQAGPASAAMKVLRSVMHQESSKIGTADKTAGILVVQADASVVVPG 361

Query: 361 NPQKLKAVEIAAAYSSLSFGMGYFASSMERPKVSILRSTKQNNKMGIDHFEACIDL 413
           +  +L AVEIAAAYSSLSFG+GY+ +S+E+PK+SILR+ +Q ++ G+DHFEA IDL
Sbjct: 362 SKPELNAVEIAAAYSSLSFGVGYYGNSIEKPKISILRTRRQMSEAGVDHFEARIDL 388

BLAST of CmaCh06G010960 vs. TAIR 10
Match: AT3G16150.1 (N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein )

HSP 1 Score: 94.7 bits (234), Expect = 1.8e-19
Identity = 89/300 (29.67%), Postives = 142/300 (47.33%), Query Frame = 0

Query: 14  VAVHVGAGF--HAPSNEKALRSAMKRACLAASLVLRKGSGGCLDAVSAAIQVLEDDPVTN 73
           +AVH GAG   + P+  +     +   CL   ++  + +   +D V   I+ LE DP+ N
Sbjct: 6   IAVHGGAGIDPNLPAERQEEAKQLLTRCLNLGIIALRSNVSAIDVVELVIRELETDPLFN 65

Query: 74  AGRGSNLTEDGRVECDASIMDGDTGAFGAVGAVPGVRNAIKIAALLAKEQILGSSLLGLI 133
           +GRGS LTE G VE +ASIMDG     GAV  +  V+N I +A L+  +           
Sbjct: 66  SGRGSALTEKGTVEMEASIMDGTKRRCGAVSGITTVKNPISLARLVMDKS---------- 125

Query: 134 PPIFLVGEGACTWAKSKGIWLPETTAEANEWLVTERAKVQWERYKSMIQRAKEKKENSYV 193
           P  +L   GA  +A+ +G+ + +     NE+ VT       +    M++ AKE   NS +
Sbjct: 126 PHSYLAFSGAEDFARKQGVEIVD-----NEYFVT-------DDNVGMLKLAKE--ANSIL 185

Query: 194 VNSCSSQDNTRISEVQSHNPLEGTTCQSATLNDAEND------CTMDTVGVICVDSEGHV 253
                   + RI       P  G    +AT +  + +         +TVG + VD +GH 
Sbjct: 186 F-------DYRI-------PPMGCAGAAATDSPIQMNGLPISIYAPETVGCVVVDGKGHC 245

Query: 254 ASGASSGGIAMKVSGRVGLAAMYGSGCWASSKGPFGTPFMVGGCVSGGGEYLMKGFAARE 306
           A+G S+GG+  K+ GR+G + + G+G +AS           G   +G GE +++   AR+
Sbjct: 246 AAGTSTGGLMNKMMGRIGDSPLIGAGTYASE--------FCGVSCTGEGEAIIRATLARD 259

BLAST of CmaCh06G010960 vs. TAIR 10
Match: AT5G08100.1 (N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein )

HSP 1 Score: 86.7 bits (213), Expect = 5.0e-17
Identity = 94/347 (27.09%), Postives = 145/347 (41.79%), Query Frame = 0

Query: 14  VAVHVGAG---FHAPSNEKALRSAMKRACLAASLVLRKGSGGCLDAVSAAIQVLEDDPVT 73
           +A+H GAG      P   +  R +  R CL   +   K     LD     ++ LE+ P  
Sbjct: 6   IALHGGAGDIPIDLPDERRIPRESALRHCLDLGISALKSGKPPLDVAELVVRELENHPDF 65

Query: 74  NAGRGSNLTEDGRVECDASIMDGDTGAFGAVGAVPGVRNAIKIAALLAKEQILGSSLLGL 133
           NAG+GS LT  G VE +ASIMDG T   GAV  +  V N I +A L+ ++          
Sbjct: 66  NAGKGSVLTAQGTVEMEASIMDGKTKRCGAVSGLTTVVNPISLARLVMEK---------- 125

Query: 134 IPPIFLVGEGACTWAKSKGIWLPETTAEANEWLVTERAKVQWERYKSMIQRAKEKKENSY 193
            P I+L  + A  +A++ G+     T +++ ++  E            I R K+ KE   
Sbjct: 126 TPHIYLAFDAAEAFARAHGV----ETVDSSHFITPEN-----------IARLKQAKE--- 185

Query: 194 VVNSCSSQDNTRISEVQSHNPLEGTTCQSATLNDAENDCTMDTVGVICVDSEGHVASGAS 253
                        + VQ        T  S  + D   D  + TVG + VDS G++AS  S
Sbjct: 186 ------------FNRVQL-----DYTVPSPKVPDNCGDSQIGTVGCVAVDSAGNLASATS 245

Query: 254 SGGIAMKVSGRVGLAAMYGSGCWASSKGPFGTPFMVGGCVSGGGEYLMKGFAARECCVSL 313
           +GG   K+ GR+G   + G+G +A+         +     +G GE +++G  AR+    +
Sbjct: 246 TGGYVNKMVGRIGDTPVIGAGTYAN--------HLCAISATGKGEDIIRGTVARDVAALM 292

Query: 314 SLSQAGPASACMKVLRNFVQESSQHNTDKSAGILLVQADAPKLAPGN 358
                    A   V+   V          S G++ V A+     P N
Sbjct: 306 EYKGLSLTEAAAYVVDQSVPRG-------SCGLVAVSANGEVTMPFN 292

BLAST of CmaCh06G010960 vs. TAIR 10
Match: AT5G08100.2 (N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein )

HSP 1 Score: 51.6 bits (122), Expect = 1.8e-06
Identity = 68/271 (25.09%), Postives = 110/271 (40.59%), Query Frame = 0

Query: 87  DASIMDGDTGAFGAVGAVPGVRNAIKIAALLAKEQILGSSLLGLIPPIFLVGEGACTWAK 146
           +ASIMDG T   GAV  +  V N I +A L+ ++           P I+L  + A  +A+
Sbjct: 2   EASIMDGKTKRCGAVSGLTTVVNPISLARLVMEK----------TPHIYLAFDAAEAFAR 61

Query: 147 SKGIWLPETTAEANEWLVTERAKVQWERYKSMIQRAKEKKENSYVVNSCSSQDNTRISEV 206
           + G+     T +++ ++  E            I R K+ KE                + V
Sbjct: 62  AHGV----ETVDSSHFITPEN-----------IARLKQAKE---------------FNRV 121

Query: 207 QSHNPLEGTTCQSATLNDAENDCTMDTVGVICVDSEGHVASGASSGGIAMKVSGRVGLAA 266
           Q        T  S  + D   D  + TVG + VDS G++AS  S+GG   K+ GR+G   
Sbjct: 122 QL-----DYTVPSPKVPDNCGDSQIGTVGCVAVDSAGNLASATSTGGYVNKMVGRIGDTP 181

Query: 267 MYGSGCWASSKGPFGTPFMVGGCVSGGGEYLMKGFAARECCVSLSLSQAGPASACMKVLR 326
           + G+G +A+         +     +G GE +++G  AR+    +         A   V+ 
Sbjct: 182 VIGAGTYAN--------HLCAISATGKGEDIIRGTVARDVAALMEYKGLSLTEAAAYVVD 212

Query: 327 NFVQESSQHNTDKSAGILLVQADAPKLAPGN 358
             V          S G++ V A+     P N
Sbjct: 242 QSVPRG-------SCGLVAVSANGEVTMPFN 212

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O652681.0e-14466.11Putative threonine aspartase OS=Arabidopsis thaliana OX=3702 GN=At4g00590 PE=2 S... [more]
Q9H6P54.6e-4436.53Threonine aspartase 1 OS=Homo sapiens OX=9606 GN=TASP1 PE=1 SV=1[more]
Q8R1G12.3e-4336.15Threonine aspartase 1 OS=Mus musculus OX=10090 GN=Tasp1 PE=2 SV=1[more]
Q9P6N72.0e-2326.85Putative threonine aspartase OS=Schizosaccharomyces pombe (strain 972 / ATCC 248... [more]
Q6GM785.0e-2234.25Isoaspartyl peptidase/L-asparaginase OS=Xenopus laevis OX=8355 GN=asrgl1 PE=2 SV... [more]
Match NameE-valueIdentityDescription
A0A6J1IIB21.1e-232100.00putative threonine aspartase OS=Cucurbita maxima OX=3661 GN=LOC111474081 PE=4 SV... [more]
A0A6J1F0Q01.3e-23099.28putative threonine aspartase OS=Cucurbita moschata OX=3662 GN=LOC111441303 PE=4 ... [more]
A0A6J1D2M38.7e-21191.87putative threonine aspartase OS=Momordica charantia OX=3673 GN=LOC111016424 PE=4... [more]
A0A0A0L7I26.9e-20889.95Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G122610 PE=4 SV=1[more]
A0A5D3D0308.4e-20689.55Putative threonine aspartase isoform X1 OS=Cucumis melo var. makuwa OX=1194695 G... [more]
Match NameE-valueIdentityDescription
XP_022975108.12.2e-232100.00putative threonine aspartase [Cucurbita maxima][more]
XP_022934036.12.7e-23099.28putative threonine aspartase [Cucurbita moschata] >KAG6597152.1 putative threoni... [more]
XP_023541014.11.0e-22999.28putative threonine aspartase [Cucurbita pepo subsp. pepo][more]
XP_022147521.11.8e-21091.87putative threonine aspartase [Momordica charantia][more]
XP_038878205.12.9e-20890.89putative threonine aspartase [Benincasa hispida][more]
Match NameE-valueIdentityDescription
AT4G00590.17.4e-14666.11N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein [more]
AT4G00590.29.7e-13061.06N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein [more]
AT3G16150.11.8e-1929.67N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein [more]
AT5G08100.15.0e-1727.09N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein [more]
AT5G08100.21.8e-0625.09N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000246Peptidase T2, asparaginase 2PFAMPF01112Asparaginase_2coord: 14..332
e-value: 4.1E-49
score: 167.3
IPR000246Peptidase T2, asparaginase 2PANTHERPTHR10188L-ASPARAGINASEcoord: 8..391
NoneNo IPR availableGENE3D3.60.20.30(Glycosyl)asparaginasecoord: 233..401
e-value: 7.0E-31
score: 109.2
NoneNo IPR availablePIRSRPIRSR600246-1PIRSR600246-1coord: 11..391
e-value: 2.4E-68
score: 228.4
NoneNo IPR availablePIRSRPIRSR600246-3PIRSR600246-3coord: 11..391
e-value: 2.4E-68
score: 228.4
NoneNo IPR availablePANTHERPTHR10188:SF8THREONINE ASPARTASE 1coord: 8..391
IPR037464Threonine aspartase 1CDDcd04514Taspase1_likecoord: 12..396
e-value: 3.43602E-124
score: 360.434
IPR029055Nucleophile aminohydrolases, N-terminalSUPERFAMILY56235N-terminal nucleophile aminohydrolases (Ntn hydrolases)coord: 11..385

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh06G010960.1CmaCh06G010960.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0020037 heme binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0004497 monooxygenase activity
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen
molecular_function GO:0004298 threonine-type endopeptidase activity
molecular_function GO:0016787 hydrolase activity