CmaCh06G010950 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh06G010950
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
DescriptionMethenyltetrahydrofolate cyclohydrolase
LocationCma_Chr06: 7394721 .. 7399011 (+)
RNA-Seq ExpressionCmaCh06G010950
SyntenyCmaCh06G010950
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AAAAAAATTTAAAATTTTAAAAATAATAAAAATAAAAGGAAACTGCTCCTATGCCGGAGTGAATAATCAGTGTTCACGTTAGGGATTCATTTTATTTCAGCCGCCACTTCTCCGCCGTCTTCTCCGCCGGCTCTCATCATCCTACACCTGTTCTGATTCCTCTCTCGGTTGATTTCGCCTACAAAAGTAAGTCTCTCTTTGTTCATCGTGAGTGACACAGAGAACGAAATAAGTGTATCTTTTTGCCTTGTTCCACAGTTTCTCTTTGCTCCGCCGCTCTGTAGCTCTGTAATTCCTGATTGTTTTTTTAGCAAAAACGTGCAGATTTTTCTCACGGTGTCTTCCAATGGCATCATCGATGAAATTCACAGATTGTTCCCCTTCCGCCATTGTTCGTCTTGTTCCCTCTACTCGAACCAGGCTTCGAAGCTTCTCCGATGGCGCTGTTCTCTTTCACCGTGTTCTTGGCCGTTCCTGTCCTGCGTATCCTAGAACTTTTGTTTCTCGATCTTCAATTTCAACTTTGCCCGTCATCTCTGGTATGTCTCTTTGTTTATGAGAAAAATTCTTTTATGATTCTTTTGTTTAGAGCTTCATAGGATCTGCCGTTTCATTTTCACCTAATTTTTCTCCTTGAACTTGTGGAACTCTGGAATGTTTTTGCGTTGATTTTATGCTGCTTGCAAGCATTCTAGCTTCCAGCGTTACTGGTGCTAAAATGGTGTTAAATTGTTGTAGTTTTGAATGCTGGCTATGCAACTTCAGAATAAGTGTTCTATGTATAGGCATGCATGTTGTGGTTGGATGATCTTGGATTGTTCATAGTTTTTTATGTACTTATTCAGTAACCATTACCATGGAGAATTCAGCTTGATTTTCATAGTTAGAGCAATGGAGAGATCTTTTTGTGTGGCTTTTATGCTAGGGATTCTGGATTCTTATTTTCTTTTTACTTCAGCTTCAATCTCGACCGAGGCAACTGCACAAGTTATTGATGGAAAATTGGTGGCGAAGCAGATAAGAGAGGAGATTACTGCTGAAGTATCTCGAATGAAGGATGCCATTGGTGTTGTTCCGGGATTAGCAGTCATCCTTGTTGGAGATAGGAAGGATTCTGCAACTTATGTACGAAACAAGAAAAAAGCTTGTGAATCTGTGGGTATTAAATCCTTTGAAGTAAACTTGCCTGAAAATGCTACCGAGCAAGAAGTGCTCAAGCATATATCCGACTTCAATGATGATCCATCTGTCCATGGCATCCTTGTTCAATTACCTTTGCCTAAGGTATGTTTACTAAACTTCATTTCATTTGTCAAATAACTTATATTGTGAGATCCACCCTCAAAGAACGAGTGTTAGCAAGGACGCTGACTCTCGAAGGGGGTAAATTGTGAGATCTCACATCGGTTGGAGAGAGAAACGAAAAACGAAGTTTTCTTTATAAGGGTATGAAAACCTCTCCCTAGCAAAAGCGTTTTAAAACTGTGAGGCTTATGACGATTTGTAACAGGCCAAAACGGACAATATCTGCTAGCGATGGGCTTGAGTAGTGTCACAATTTACTATATTATCTTAGAATATTACTAAGGACACTCTAAACTTAAGACCTCGGTCTATAATCATTTGCTTGTTTGCTTTTTGTTTTTGAAAATTATGTTCACAAGCACTTCTTCCACATATGAGTTTCTATGTTTTTTTAATCTAGAAACCAAACAAAAGGGCTTAGAAGGAATGAGTTCGAACAGGGTGGCTACCTGCCTAGGATTTAATACCCTACGAGATTCTTTGACAACCAAATATTGTAGGTCAAGTGGTCGTCCCATGATATTAGTTGAAGTGTGCGCAAGTTGGCTCAGACACCCACGAATGTTAAAAAAGAACCAATTTTAAAAACGTGTTTTTTTATTAATCTCGCTGCTTGGCCTGGCCTGGCTGGCCCTTCCCTCAGCTCTTCAAGCATACTCTGCTCCTTCTTTCTTCCCTCTCCTCTTCGAGCGAGCATACTCAATCCTTCTTTCTTCCCTCTCCTCTTCTCGTCTAGCTGCTTCCTGCTTCGCTGCTTTCTTTCTTCCCTCTCCCCAGTGTTCCTTGTCTCACTGCTTTGCTTCTTCCCTCTCCTCTCTTTTACAGTCAAGTCGCTTCCCGCCTCTTCGAGTCGACCGGTTCTGGAGCCAGCGGGTTATGTGAATGAACTCCCCCTTACGGTTAAAAAAGTAGTTAACCATCTCGAAGAAATCCAAAGTACGACCAAACTGAGAGTGAGAGTTTCGGGGGGCGGTTAGGCATCCTTAGACGAAAGAGTGGTCTTTCAAGATTGGTCGTTGTGTGTCACCACCTCCCACCTCTCCTACACATTTGATCAAGGTTGTCACTGCGAAGCTATAGTTAAGGTGCACGGGGTCTTACCGTCTAGCCGTTGGTACTCCGCATCTTCACGGAGAATTCAATTTCACCGGGTCCATGTCGGAGATAGTGGGGCAGTCGTTACACCATTCGTGCAGGTCGCTACTTATGCGACAAGGAATTTCGCTACCTTAGGACAGTTAGAGTTACTGCCGCCATTTACTGGGGCTTCCATTCAAAGCTTATAACACTTCTCCTTCCAACCTTTCAGCACCGAGCAGATTTCAAACTCTATACATCATGTTACCACTTAGCAGAGTTCTATATTTTTAATAAACAGTGGGCCCTTGGTATGTGGCGCTTTCCTAATCAAAAGATAGGAGAGCACTCTTCTAGAACTTGCTAAGAATTCAACTTAAAACCATAGTAAAAAAATGGAGAGAAAACAATTACTAATTAAACTTCGAAACATGCATAGTAAAAAAAAATTGTTATCGAACGGTGTGGCCATGTCGTTCGTTGGGTGCTGCTTATTTTATTAAATAAGTTTTGTTGGTATCTGCATTATTTGTTTGCTATCTTCATTTCCACATGTCTTATCCTATGTAAATATAGATGAAAGCTCGAACTTATATGCATATTTTGTTTAGGGTAATGGGTACCTGTTAATTACCCGTTGAACTGTACTCACTTTGGCAACGGGAAAAATGTTTATAGCAGTATTATGGTGCAATGGACCCCATTACATTAGCTATTCAAGATCCAACATAACATGTTTTCATTGATTTTGGCAGCATATGAATGAACAGAATGTATTACATGCTGTTAGGATTGAAAAAGACGTAGATGGATTCCACCCATTGAACATTGGTCGTCTTGCAATGAGAGGTGAAGAACCGTTGTTTGTTCCATGCACACCAAAAGGGTGTATTGAATTGCTGCATAGATACAACATTAGTATCAAAGGAAAGAGAGCTGTTGTAATCGGTCGAAGCAATATTGTAGGAACTCCAGCTGCTCTTTTGCTTCAAGTGAGTTTTTAATTCTTAATCTATCTACTTCACAACGTTATTGATTGTTGATTCTCTTGACGATAATGTTTATCGTTCTTGTGTAGCGAGAAGATGCTACCGTTAGCGTAGTTCATTCGAGGACCAAGGACCCCGAAGAGTTAACAAGACAAGCTGATATTATCATCTCAGCAGTTGGCAAAGCTAATATGGTTGAGGGAAGTTGGATAAAGCCTGGTGCGGTTATTATTGATGTTGGAATTAATCCGGTAGAGGTGAGTAAAGTACTTTTACACAAACATTTATTATCGTTAAACACCTTTTAAAGGATTACGTTGTACAATTAGATCGAATAAGTCCTCGTTCTCTTTGATCTTCGTGACATGTCTATGTACATATGGCTAAACCGAAATCTTCTCTCATTTCTACATTTGAAGTTTCACACTCGGTTGTCATCTTCGTGTAACAGGATGCAAGTAGTCCTCGTGGATATCGACTCGTTGGGGATGTCTGTTATGAGGAGGCCAGCAAAATTGCTTCGGCTATTACTCCTGTACCCGGGGGAGTCGGGCCAATGACGATAGCGATGCTCCTCTCGAACACACTCACAGCAGCAAAGAGAAAACACAACTTCCAGTGAAATTTACCTAAAAAGCTTTCCTTTAGCAAAGGACCAAGCTGTTAAATAACCCCATGTGTGAGCACACATCTTTTCAAGTCATTACAACTCATCTTCTTTTGGGTGAATGAATGACTTTTGGAGATTGAACCTTGTATTTGTTGGATCTTTTCTATATGCTGAATAATCACAGGGGATACACATTTTGTTGTTGCATTGTCGTATCGAGGTCTAGTTAGGGGTGCAAATTATAACGAACAAGACGGATTGAAATAGAAATTGATCGGTCGGGATGGAAAGATGAATGGTTGAGATGATG

mRNA sequence

AAAAAAATTTAAAATTTTAAAAATAATAAAAATAAAAGGAAACTGCTCCTATGCCGGAGTGAATAATCAGTGTTCACGTTAGGGATTCATTTTATTTCAGCCGCCACTTCTCCGCCGTCTTCTCCGCCGGCTCTCATCATCCTACACCTGTTCTGATTCCTCTCTCGGTTGATTTCGCCTACAAAACAAAAACGTGCAGATTTTTCTCACGGTGTCTTCCAATGGCATCATCGATGAAATTCACAGATTGTTCCCCTTCCGCCATTGTTCGTCTTGTTCCCTCTACTCGAACCAGGCTTCGAAGCTTCTCCGATGGCGCTGTTCTCTTTCACCGTGTTCTTGGCCGTTCCTGTCCTGCGTATCCTAGAACTTTTGTTTCTCGATCTTCAATTTCAACTTTGCCCGTCATCTCTGCTTCAATCTCGACCGAGGCAACTGCACAAGTTATTGATGGAAAATTGGTGGCGAAGCAGATAAGAGAGGAGATTACTGCTGAAGTATCTCGAATGAAGGATGCCATTGGTGTTGTTCCGGGATTAGCAGTCATCCTTGTTGGAGATAGGAAGGATTCTGCAACTTATGTACGAAACAAGAAAAAAGCTTGTGAATCTGTGGGTATTAAATCCTTTGAAGTAAACTTGCCTGAAAATGCTACCGAGCAAGAAGTGCTCAAGCATATATCCGACTTCAATGATGATCCATCTGTCCATGGCATCCTTGTTCAATTACCTTTGCCTAAGCATATGAATGAACAGAATGTATTACATGCTGTTAGGATTGAAAAAGACGTAGATGGATTCCACCCATTGAACATTGGTCGTCTTGCAATGAGAGGTGAAGAACCGTTGTTTGTTCCATGCACACCAAAAGGGTGTATTGAATTGCTGCATAGATACAACATTAGTATCAAAGGAAAGAGAGCTGTTGTAATCGGTCGAAGCAATATTGTAGGAACTCCAGCTGCTCTTTTGCTTCAACGAGAAGATGCTACCGTTAGCGTAGTTCATTCGAGGACCAAGGACCCCGAAGAGTTAACAAGACAAGCTGATATTATCATCTCAGCAGTTGGCAAAGCTAATATGGTTGAGGGAAGTTGGATAAAGCCTGGTGCGGTTATTATTGATGTTGGAATTAATCCGGTAGAGGATGCAAGTAGTCCTCGTGGATATCGACTCGTTGGGGATGTCTGTTATGAGGAGGCCAGCAAAATTGCTTCGGCTATTACTCCTGTACCCGGGGGAGTCGGGCCAATGACGATAGCGATGCTCCTCTCGAACACACTCACAGCAGCAAAGAGAAAACACAACTTCCAGTGAAATTTACCTAAAAAGCTTTCCTTTAGCAAAGGACCAAGCTGTTAAATAACCCCATGTGTGAGCACACATCTTTTCAAGTCATTACAACTCATCTTCTTTTGGGTGAATGAATGACTTTTGGAGATTGAACCTTGTATTTGTTGGATCTTTTCTATATGCTGAATAATCACAGGGGATACACATTTTGTTGTTGCATTGTCGTATCGAGGTCTAGTTAGGGGTGCAAATTATAACGAACAAGACGGATTGAAATAGAAATTGATCGGTCGGGATGGAAAGATGAATGGTTGAGATGATG

Coding sequence (CDS)

ATGGCATCATCGATGAAATTCACAGATTGTTCCCCTTCCGCCATTGTTCGTCTTGTTCCCTCTACTCGAACCAGGCTTCGAAGCTTCTCCGATGGCGCTGTTCTCTTTCACCGTGTTCTTGGCCGTTCCTGTCCTGCGTATCCTAGAACTTTTGTTTCTCGATCTTCAATTTCAACTTTGCCCGTCATCTCTGCTTCAATCTCGACCGAGGCAACTGCACAAGTTATTGATGGAAAATTGGTGGCGAAGCAGATAAGAGAGGAGATTACTGCTGAAGTATCTCGAATGAAGGATGCCATTGGTGTTGTTCCGGGATTAGCAGTCATCCTTGTTGGAGATAGGAAGGATTCTGCAACTTATGTACGAAACAAGAAAAAAGCTTGTGAATCTGTGGGTATTAAATCCTTTGAAGTAAACTTGCCTGAAAATGCTACCGAGCAAGAAGTGCTCAAGCATATATCCGACTTCAATGATGATCCATCTGTCCATGGCATCCTTGTTCAATTACCTTTGCCTAAGCATATGAATGAACAGAATGTATTACATGCTGTTAGGATTGAAAAAGACGTAGATGGATTCCACCCATTGAACATTGGTCGTCTTGCAATGAGAGGTGAAGAACCGTTGTTTGTTCCATGCACACCAAAAGGGTGTATTGAATTGCTGCATAGATACAACATTAGTATCAAAGGAAAGAGAGCTGTTGTAATCGGTCGAAGCAATATTGTAGGAACTCCAGCTGCTCTTTTGCTTCAACGAGAAGATGCTACCGTTAGCGTAGTTCATTCGAGGACCAAGGACCCCGAAGAGTTAACAAGACAAGCTGATATTATCATCTCAGCAGTTGGCAAAGCTAATATGGTTGAGGGAAGTTGGATAAAGCCTGGTGCGGTTATTATTGATGTTGGAATTAATCCGGTAGAGGATGCAAGTAGTCCTCGTGGATATCGACTCGTTGGGGATGTCTGTTATGAGGAGGCCAGCAAAATTGCTTCGGCTATTACTCCTGTACCCGGGGGAGTCGGGCCAATGACGATAGCGATGCTCCTCTCGAACACACTCACAGCAGCAAAGAGAAAACACAACTTCCAGTGA

Protein sequence

MASSMKFTDCSPSAIVRLVPSTRTRLRSFSDGAVLFHRVLGRSCPAYPRTFVSRSSISTLPVISASISTEATAQVIDGKLVAKQIREEITAEVSRMKDAIGVVPGLAVILVGDRKDSATYVRNKKKACESVGIKSFEVNLPENATEQEVLKHISDFNDDPSVHGILVQLPLPKHMNEQNVLHAVRIEKDVDGFHPLNIGRLAMRGEEPLFVPCTPKGCIELLHRYNISIKGKRAVVIGRSNIVGTPAALLLQREDATVSVVHSRTKDPEELTRQADIIISAVGKANMVEGSWIKPGAVIIDVGINPVEDASSPRGYRLVGDVCYEEASKIASAITPVPGGVGPMTIAMLLSNTLTAAKRKHNFQ
Homology
BLAST of CmaCh06G010950 vs. ExPASy Swiss-Prot
Match: O65271 (Bifunctional protein FolD 4, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=FOLD4 PE=1 SV=1)

HSP 1 Score: 528.9 bits (1361), Expect = 4.6e-149
Identity = 274/365 (75.07%), Postives = 307/365 (84.11%), Query Frame = 0

Query: 3   SSMKFTDCSPSAIVRLVPSTRTRLRSFSDGAVLFHRVLGR---SCPAYPRTFVSRSSIST 62
           +SM FTDCS +   RL+   R      S G  L  + +G+      A  R    RSS S 
Sbjct: 2   ASMMFTDCSSTTTSRLIHLNR------SSGTFLLRQCVGQLRLQTTASGRGCCIRSSSSP 61

Query: 63  LPVISASISTEATAQVIDGKLVAKQIREEITAEVSRMKDAIGVVPGLAVILVGDRKDSAT 122
           +  ISA   +E  A VIDGK VAK+IR+EIT EVSRMK++IGV+PGLAVILVGDRKDSAT
Sbjct: 62  ISSISADTKSEGGAIVIDGKAVAKKIRDEITIEVSRMKESIGVIPGLAVILVGDRKDSAT 121

Query: 123 YVRNKKKACESVGIKSFEVNLPENATEQEVLKHISDFNDDPSVHGILVQLPLPKHMNEQN 182
           YVRNKKKAC+SVGIKSFEV L E+++E+EVLK +S FNDDPSVHGILVQLPLP HM+EQN
Sbjct: 122 YVRNKKKACDSVGIKSFEVRLAEDSSEEEVLKSVSGFNDDPSVHGILVQLPLPSHMDEQN 181

Query: 183 VLHAVRIEKDVDGFHPLNIGRLAMRGEEPLFVPCTPKGCIELLHRYNISIKGKRAVVIGR 242
           +L+AV IEKDVDGFHPLNIGRLAMRG EPLFVPCTPKGCIELLHRYNI IKGKRAVVIGR
Sbjct: 182 ILNAVSIEKDVDGFHPLNIGRLAMRGREPLFVPCTPKGCIELLHRYNIEIKGKRAVVIGR 241

Query: 243 SNIVGTPAALLLQREDATVSVVHSRTKDPEELTRQADIIISAVGKANMVEGSWIKPGAVI 302
           SNIVG PAALLLQREDATVS++HSRTK+PEE+TR+ADIIISAVG+ NMV GSWIKPGAV+
Sbjct: 242 SNIVGMPAALLLQREDATVSIIHSRTKNPEEITREADIIISAVGQPNMVRGSWIKPGAVL 301

Query: 303 IDVGINPVEDASSPRGYRLVGDVCYEEASKIASAITPVPGGVGPMTIAMLLSNTLTAAKR 362
           IDVGINPVED S+ RGYRLVGD+CYEEASK+ASAITPVPGGVGPMTIAMLLSNTLT+AKR
Sbjct: 302 IDVGINPVEDPSAARGYRLVGDICYEEASKVASAITPVPGGVGPMTIAMLLSNTLTSAKR 360

Query: 363 KHNFQ 365
            HNFQ
Sbjct: 362 IHNFQ 360

BLAST of CmaCh06G010950 vs. ExPASy Swiss-Prot
Match: O65269 (Bifunctional protein FolD 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=FOLD3 PE=3 SV=1)

HSP 1 Score: 411.8 bits (1057), Expect = 8.3e-114
Identity = 225/363 (61.98%), Postives = 257/363 (70.80%), Query Frame = 0

Query: 7   FTDCSPSAIVRLVPSTRTRLRSFSDGAVLFHRVLGRSCPAYPRTFVSRSSISTLP----- 66
           FTDCS S   RL+                   +  R+    PR  VS+ S+ T       
Sbjct: 2   FTDCSSSTTSRLI------------------HLYNRNGVFLPRPSVSQFSLRTTASTWRC 61

Query: 67  VISASISTEATAQVIDGKLVAKQIREEITAEVSRMKDAIGVVPGLAVILVGDRKDSATYV 126
            +S   S+  +A VIDGK  AK+IR++I  EVSRMK++IGVVP                 
Sbjct: 62  TLSIRSSSSPSAIVIDGKAEAKKIRDDIKIEVSRMKESIGVVPA---------------- 121

Query: 127 RNKKKACESVGIKSFEVNLPENATEQEVLKHISDFNDDPSVHGILVQLPLPKHMNEQNVL 186
                               E+++E+EVLK++S FNDDPSVHG+LVQLPLP HM+EQN+L
Sbjct: 122 --------------------EDSSEEEVLKYVSGFNDDPSVHGVLVQLPLPSHMDEQNIL 181

Query: 187 HAVRIEKDVDGFHPLNIGRLAMRGEEPLFVPCTPKGCIELLHRYNISIKGKRAVVIGRSN 246
           +AV IEKDVDGFHPLNIGRLAMRG EPLFVPCTPKGCIELLHRYNI  KGKRAVVIGRSN
Sbjct: 182 NAVSIEKDVDGFHPLNIGRLAMRGREPLFVPCTPKGCIELLHRYNIEFKGKRAVVIGRSN 241

Query: 247 IVGTPAALLLQREDATVSVVHSRTKDPEELTRQADIIISAVGKANMVEGSWIKPGAVIID 306
           IVG PAALLLQ+EDATVS++HSRT +PEELTRQADI+ISAVGK NMV GSWIKPGAV+ID
Sbjct: 242 IVGMPAALLLQKEDATVSIIHSRTMNPEELTRQADILISAVGKPNMVRGSWIKPGAVLID 301

Query: 307 VGINPVEDASSPRGYRLVGDVCYEEASKIASAITPVPGGVGPMTIAMLLSNTLTAAKRKH 365
           VGI PVED S+  G RLVGD+CY EASKIASAITPVPG VGPMTIAMLLSNTLT+AKR H
Sbjct: 302 VGIKPVEDPSAAGGERLVGDICYVEASKIASAITPVPGDVGPMTIAMLLSNTLTSAKRIH 310

BLAST of CmaCh06G010950 vs. ExPASy Swiss-Prot
Match: A2RVV7 (Bifunctional protein FolD 1, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=FOLD1 PE=2 SV=1)

HSP 1 Score: 403.3 bits (1035), Expect = 2.9e-111
Identity = 198/299 (66.22%), Postives = 236/299 (78.93%), Query Frame = 0

Query: 61  PVISASISTEATAQVIDGKLVAKQIREEITAEVSRMKDAIGVVPGLAVILVGDRKDSATY 120
           P    S  TE    VIDG ++A++IR +I +EV +MK A+G VPGLAV+LVG+++DS TY
Sbjct: 51  PPPPVSFETEQKTVVIDGNVIAEEIRTKIISEVGKMKKAVGKVPGLAVVLVGEQRDSQTY 110

Query: 121 VRNKKKACESVGIKSFEVNLPENATEQEVLKHISDFNDDPSVHGILVQLPLPKHMNEQNV 180
           VRNK KACE  GIKS    LPE+ TE +++  +  FN+D S+HGILVQLPLP+H+NE  +
Sbjct: 111 VRNKIKACEETGIKSVLAELPEDCTEGQIISVLRKFNEDTSIHGILVQLPLPQHLNESKI 170

Query: 181 LHAVRIEKDVDGFHPLNIGRLAMRGEEPLFVPCTPKGCIELLHRYNISIKGKRAVVIGRS 240
           L+ VR+EKDVDGFHPLN+G LAMRG EPLFV CTPKGC+ELL R  + I GK AVVIGRS
Sbjct: 171 LNMVRLEKDVDGFHPLNVGNLAMRGREPLFVSCTPKGCVELLIRTGVEIAGKNAVVIGRS 230

Query: 241 NIVGTPAALLLQREDATVSVVHSRTKDPEELTRQADIIISAVGKANMVEGSWIKPGAVII 300
           NIVG P +LLLQR DATVS VH+ TKDPE +TR+ADI+I+A G  N+V GSW+KPGAV+I
Sbjct: 231 NIVGLPMSLLLQRHDATVSTVHAFTKDPEHITRKADIVIAAAGIPNLVRGSWLKPGAVVI 290

Query: 301 DVGINPVEDASSPRGYRLVGDVCYEEASKIASAITPVPGGVGPMTIAMLLSNTLTAAKR 360
           DVG  PVED+S   GYRLVGDVCYEEA  +ASAITPVPGGVGPMTI MLL NTL AAKR
Sbjct: 291 DVGTTPVEDSSCEFGYRLVGDVCYEEALGVASAITPVPGGVGPMTITMLLCNTLEAAKR 349

BLAST of CmaCh06G010950 vs. ExPASy Swiss-Prot
Match: Q9LHH7 (Bifunctional protein FolD 2 OS=Arabidopsis thaliana OX=3702 GN=FOLD2 PE=2 SV=1)

HSP 1 Score: 387.9 bits (995), Expect = 1.3e-106
Identity = 184/292 (63.01%), Postives = 232/292 (79.45%), Query Frame = 0

Query: 68  STEATAQVIDGKLVAKQIREEITAEVSRMKDAIGVVPGLAVILVGDRKDSATYVRNKKKA 127
           S++ TA++IDGK +A  IR EI  EV  + +  G VPGLAV++VG RKDS TYV  K+KA
Sbjct: 4   SSDHTAKIIDGKAIAHTIRSEIAEEVRGLSEKHGKVPGLAVVIVGSRKDSQTYVNTKRKA 63

Query: 128 CESVGIKSFEVNLPENATEQEVLKHISDFNDDPSVHGILVQLPLPKHMNEQNVLHAVRIE 187
           C  VGIKSF+V LPE  +E +++  + + N +P VHGILVQLPLPKH+NE+++L A+ I+
Sbjct: 64  CAEVGIKSFDVGLPEEVSEADLISKVHELNSNPDVHGILVQLPLPKHINEEHILGAISID 123

Query: 188 KDVDGFHPLNIGRLAMRGEEPLFVPCTPKGCIELLHRYNISIKGKRAVVIGRSNIVGTPA 247
           KDVDGFHPLNIG+LAM+G EPLF+PCTPKGC+ELL R  + IKG+RAVV+GRSNIVG P 
Sbjct: 124 KDVDGFHPLNIGKLAMKGREPLFLPCTPKGCLELLARSGVKIKGQRAVVVGRSNIVGLPV 183

Query: 248 ALLLQREDATVSVVHSRTKDPEELTRQADIIISAVGKANMVEGSWIKPGAVIIDVGINPV 307
           +LLL + DATV+ VHS TKDPE + R+ADI+I+A G+A+M++G+WIKPGA +IDVG N V
Sbjct: 184 SLLLLKADATVTTVHSHTKDPEAIIREADIVIAACGQAHMIKGNWIKPGAAVIDVGTNAV 243

Query: 308 EDASSPRGYRLVGDVCYEEASKIASAITPVPGGVGPMTIAMLLSNTLTAAKR 360
            D S   GYRLVGDV + EASK+A  ITPVPGGVGPMT+AMLL NT+  AKR
Sbjct: 244 SDPSKKSGYRLVGDVDFAEASKVAGFITPVPGGVGPMTVAMLLRNTVDGAKR 295

BLAST of CmaCh06G010950 vs. ExPASy Swiss-Prot
Match: A0LE04 (Bifunctional protein FolD OS=Magnetococcus marinus (strain ATCC BAA-1437 / JCM 17883 / MC-1) OX=156889 GN=folD PE=3 SV=1)

HSP 1 Score: 310.5 bits (794), Expect = 2.6e-83
Identity = 165/290 (56.90%), Postives = 199/290 (68.62%), Query Frame = 0

Query: 73  AQVIDGKLVAKQIREEITAEVSRMKDAIGVVPGLAVILVGDRKDSATYVRNKKKACESVG 132
           A VIDGK +A+ +REE+  EV R+K    + PGLAV+LVG    S  YVRNKK+ACE+ G
Sbjct: 2   AHVIDGKAIAQSVREELRMEVERLKLNHQLTPGLAVVLVGADPASQVYVRNKKRACETAG 61

Query: 133 IKSFEVNLPENATEQEVLKHISDFNDDPSVHGILVQLPLPKHMNEQNVLHAVRIEKDVDG 192
           I SF   L    ++ E+L  I   N D +VHGILVQLPLPKH++EQ VL A+   KD DG
Sbjct: 62  IASFSHELAATTSQAELLALIEQLNQDDAVHGILVQLPLPKHIDEQKVLEAISPSKDADG 121

Query: 193 FHPLNIGRLAMRGEEPLFVPCTPKGCIELLHRYNISIKGKRAVVIGRSNIVGTPAALLLQ 252
           FHP N+GRL     +P F PCTP G +E+L    +  KGK AVVIGRSNIVG P AL+L 
Sbjct: 122 FHPYNVGRLVT--GDPTFQPCTPWGVMEMLKVSGVDPKGKHAVVIGRSNIVGKPVALMLL 181

Query: 253 REDATVSVVHSRTKDPEELTRQADIIISAVGKANMVEGSWIKPGAVIIDVGINPVEDASS 312
              ATV++ HSRT D  E  ++ADI+++AVG+ANMV GSWIK GAV+IDVGIN  ED   
Sbjct: 182 AAHATVTICHSRTPDLAETVKRADIVVAAVGRANMVPGSWIKKGAVVIDVGINRGEDG-- 241

Query: 313 PRGYRLVGDVCYEEASKIASAITPVPGGVGPMTIAMLLSNTLTAAKRKHN 363
               +L GDV Y    + ASAITPVPGGVGPMTIAMLL NT+  AKR HN
Sbjct: 242 ----KLCGDVDYASCFEHASAITPVPGGVGPMTIAMLLKNTVEGAKRAHN 283

BLAST of CmaCh06G010950 vs. ExPASy TrEMBL
Match: A0A6J1IDA1 (Methenyltetrahydrofolate cyclohydrolase OS=Cucurbita maxima OX=3661 GN=LOC111474082 PE=3 SV=1)

HSP 1 Score: 709.9 bits (1831), Expect = 5.4e-201
Identity = 364/364 (100.00%), Postives = 364/364 (100.00%), Query Frame = 0

Query: 1   MASSMKFTDCSPSAIVRLVPSTRTRLRSFSDGAVLFHRVLGRSCPAYPRTFVSRSSISTL 60
           MASSMKFTDCSPSAIVRLVPSTRTRLRSFSDGAVLFHRVLGRSCPAYPRTFVSRSSISTL
Sbjct: 1   MASSMKFTDCSPSAIVRLVPSTRTRLRSFSDGAVLFHRVLGRSCPAYPRTFVSRSSISTL 60

Query: 61  PVISASISTEATAQVIDGKLVAKQIREEITAEVSRMKDAIGVVPGLAVILVGDRKDSATY 120
           PVISASISTEATAQVIDGKLVAKQIREEITAEVSRMKDAIGVVPGLAVILVGDRKDSATY
Sbjct: 61  PVISASISTEATAQVIDGKLVAKQIREEITAEVSRMKDAIGVVPGLAVILVGDRKDSATY 120

Query: 121 VRNKKKACESVGIKSFEVNLPENATEQEVLKHISDFNDDPSVHGILVQLPLPKHMNEQNV 180
           VRNKKKACESVGIKSFEVNLPENATEQEVLKHISDFNDDPSVHGILVQLPLPKHMNEQNV
Sbjct: 121 VRNKKKACESVGIKSFEVNLPENATEQEVLKHISDFNDDPSVHGILVQLPLPKHMNEQNV 180

Query: 181 LHAVRIEKDVDGFHPLNIGRLAMRGEEPLFVPCTPKGCIELLHRYNISIKGKRAVVIGRS 240
           LHAVRIEKDVDGFHPLNIGRLAMRGEEPLFVPCTPKGCIELLHRYNISIKGKRAVVIGRS
Sbjct: 181 LHAVRIEKDVDGFHPLNIGRLAMRGEEPLFVPCTPKGCIELLHRYNISIKGKRAVVIGRS 240

Query: 241 NIVGTPAALLLQREDATVSVVHSRTKDPEELTRQADIIISAVGKANMVEGSWIKPGAVII 300
           NIVGTPAALLLQREDATVSVVHSRTKDPEELTRQADIIISAVGKANMVEGSWIKPGAVII
Sbjct: 241 NIVGTPAALLLQREDATVSVVHSRTKDPEELTRQADIIISAVGKANMVEGSWIKPGAVII 300

Query: 301 DVGINPVEDASSPRGYRLVGDVCYEEASKIASAITPVPGGVGPMTIAMLLSNTLTAAKRK 360
           DVGINPVEDASSPRGYRLVGDVCYEEASKIASAITPVPGGVGPMTIAMLLSNTLTAAKRK
Sbjct: 301 DVGINPVEDASSPRGYRLVGDVCYEEASKIASAITPVPGGVGPMTIAMLLSNTLTAAKRK 360

Query: 361 HNFQ 365
           HNFQ
Sbjct: 361 HNFQ 364

BLAST of CmaCh06G010950 vs. ExPASy TrEMBL
Match: A0A6J1GAU1 (Methenyltetrahydrofolate cyclohydrolase OS=Cucurbita moschata OX=3662 GN=LOC111452457 PE=3 SV=1)

HSP 1 Score: 696.0 bits (1795), Expect = 8.1e-197
Identity = 360/364 (98.90%), Postives = 360/364 (98.90%), Query Frame = 0

Query: 1   MASSMKFTDCSPSAIVRLVPSTRTRLRSFSDGAVLFHRVLGRSCPAYPRTFVSRSSISTL 60
           MASSMKFTDCSPSAIVRLVPSTRTRLRS SD AVLFHRVLGRSCPAYPRTFVSRSSISTL
Sbjct: 1   MASSMKFTDCSPSAIVRLVPSTRTRLRS-SDSAVLFHRVLGRSCPAYPRTFVSRSSISTL 60

Query: 61  PVISASISTEATAQVIDGKLVAKQIREEITAEVSRMKDAIGVVPGLAVILVGDRKDSATY 120
           PVISASISTEATAQVIDGKLVAKQIREEITAEVSRMKDAIGVVPGLAVILVGDRKDSATY
Sbjct: 61  PVISASISTEATAQVIDGKLVAKQIREEITAEVSRMKDAIGVVPGLAVILVGDRKDSATY 120

Query: 121 VRNKKKACESVGIKSFEVNLPENATEQEVLKHISDFNDDPSVHGILVQLPLPKHMNEQNV 180
           VRNKKKACESVGIKSFEVNLPENATEQEVLKHISDFNDDPSVHGILVQLPLPKHMNEQNV
Sbjct: 121 VRNKKKACESVGIKSFEVNLPENATEQEVLKHISDFNDDPSVHGILVQLPLPKHMNEQNV 180

Query: 181 LHAVRIEKDVDGFHPLNIGRLAMRGEEPLFVPCTPKGCIELLHRYNISIKGKRAVVIGRS 240
           LHAV IEKDVDGFHPLNIGRLAMRGEEPLFVPCTPKGCIELLHRYNISIKGKRAVVIGRS
Sbjct: 181 LHAVSIEKDVDGFHPLNIGRLAMRGEEPLFVPCTPKGCIELLHRYNISIKGKRAVVIGRS 240

Query: 241 NIVGTPAALLLQREDATVSVVHSRTKDPEELTRQADIIISAVGKANMVEGSWIKPGAVII 300
           NIVGTPAALLLQREDATVSVVHSRTKDPEELTRQADIIISAVGKANMVEGSWIKPGAVII
Sbjct: 241 NIVGTPAALLLQREDATVSVVHSRTKDPEELTRQADIIISAVGKANMVEGSWIKPGAVII 300

Query: 301 DVGINPVEDASSPRGYRLVGDVCYEEASKIASAITPVPGGVGPMTIAMLLSNTLTAAKRK 360
           DVGINPVEDASSPRGYRLVGDVCYEEASKIASAITPVPGGVGPMTIAMLLSNTLTAAKR 
Sbjct: 301 DVGINPVEDASSPRGYRLVGDVCYEEASKIASAITPVPGGVGPMTIAMLLSNTLTAAKRT 360

Query: 361 HNFQ 365
           HNFQ
Sbjct: 361 HNFQ 363

BLAST of CmaCh06G010950 vs. ExPASy TrEMBL
Match: A0A0A0L8Y4 (Methenyltetrahydrofolate cyclohydrolase OS=Cucumis sativus OX=3659 GN=Csa_3G122600 PE=3 SV=1)

HSP 1 Score: 664.1 bits (1712), Expect = 3.4e-187
Identity = 343/364 (94.23%), Postives = 350/364 (96.15%), Query Frame = 0

Query: 1   MASSMKFTDCSPSAIVRLVPSTRTRLRSFSDGAVLFHRVLGRSCPAYPRTFVSRSSISTL 60
           MASSMKFTDCSPS  VRL PS RTRL SF DGAVLFHR+L RS PA+PRTFVSRSS STL
Sbjct: 1   MASSMKFTDCSPSTTVRLFPSARTRLPSF-DGAVLFHRLLDRSRPAFPRTFVSRSSASTL 60

Query: 61  PVISASISTEATAQVIDGKLVAKQIREEITAEVSRMKDAIGVVPGLAVILVGDRKDSATY 120
           PVISASISTEATA+VIDGKLVAKQIREEITAEVSRMKDAIGVVPGLAVILVGDRKDSATY
Sbjct: 61  PVISASISTEATAEVIDGKLVAKQIREEITAEVSRMKDAIGVVPGLAVILVGDRKDSATY 120

Query: 121 VRNKKKACESVGIKSFEVNLPENATEQEVLKHISDFNDDPSVHGILVQLPLPKHMNEQNV 180
           VRNKKKACESVGIKSFEVNLPE+ TEQEVLK+ISDFNDDPSVHGILVQLPLPKHMNEQN+
Sbjct: 121 VRNKKKACESVGIKSFEVNLPEDCTEQEVLKYISDFNDDPSVHGILVQLPLPKHMNEQNI 180

Query: 181 LHAVRIEKDVDGFHPLNIGRLAMRGEEPLFVPCTPKGCIELLHRYNISIKGKRAVVIGRS 240
           LHAV IEKDVDGFHPLNIGRLAMRGEEPLFVPCTPKGCIELLHRYNI IKGKRAVVIGRS
Sbjct: 181 LHAVSIEKDVDGFHPLNIGRLAMRGEEPLFVPCTPKGCIELLHRYNIGIKGKRAVVIGRS 240

Query: 241 NIVGTPAALLLQREDATVSVVHSRTKDPEELTRQADIIISAVGKANMVEGSWIKPGAVII 300
           NIVGTPAALLLQ EDATVSVVHSRTKDPEELTRQADIIISAVGKANMV+GSWIKPGAVII
Sbjct: 241 NIVGTPAALLLQGEDATVSVVHSRTKDPEELTRQADIIISAVGKANMVKGSWIKPGAVII 300

Query: 301 DVGINPVEDASSPRGYRLVGDVCYEEASKIASAITPVPGGVGPMTIAMLLSNTLTAAKRK 360
           DVGINPVEDASSPRGYRLVGDVCYEEASKIASAITPVPGGVGPMTIAMLLSNTLTAAKR 
Sbjct: 301 DVGINPVEDASSPRGYRLVGDVCYEEASKIASAITPVPGGVGPMTIAMLLSNTLTAAKRT 360

Query: 361 HNFQ 365
           HNFQ
Sbjct: 361 HNFQ 363

BLAST of CmaCh06G010950 vs. ExPASy TrEMBL
Match: A0A5A7TZL1 (Methenyltetrahydrofolate cyclohydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold434G004520 PE=3 SV=1)

HSP 1 Score: 663.7 bits (1711), Expect = 4.5e-187
Identity = 342/364 (93.96%), Postives = 351/364 (96.43%), Query Frame = 0

Query: 1   MASSMKFTDCSPSAIVRLVPSTRTRLRSFSDGAVLFHRVLGRSCPAYPRTFVSRSSISTL 60
           MASSMKFTDCSPSA VRL PS RTR RS SDGAVLFHR+L RS PA+PRTFVSRSS STL
Sbjct: 1   MASSMKFTDCSPSATVRLFPSARTRFRS-SDGAVLFHRLLDRSRPAFPRTFVSRSSASTL 60

Query: 61  PVISASISTEATAQVIDGKLVAKQIREEITAEVSRMKDAIGVVPGLAVILVGDRKDSATY 120
           PVISASIST++TA+VIDGKLVAKQIREEITAEVSRMKDAIGVVPGLAVILVGDRKDSATY
Sbjct: 61  PVISASISTQSTAEVIDGKLVAKQIREEITAEVSRMKDAIGVVPGLAVILVGDRKDSATY 120

Query: 121 VRNKKKACESVGIKSFEVNLPENATEQEVLKHISDFNDDPSVHGILVQLPLPKHMNEQNV 180
           VRNKKKACESVGIKSFEVNLPE+ TEQEVLK+ISDFNDDPSVHGILVQLPLPKHMNEQN+
Sbjct: 121 VRNKKKACESVGIKSFEVNLPEDCTEQEVLKYISDFNDDPSVHGILVQLPLPKHMNEQNI 180

Query: 181 LHAVRIEKDVDGFHPLNIGRLAMRGEEPLFVPCTPKGCIELLHRYNISIKGKRAVVIGRS 240
           LHAV IEKDVDGFHPLNIGRLAMRGEEPLFVPCTPKGCIELLHRYNI IKGKRAVVIGRS
Sbjct: 181 LHAVSIEKDVDGFHPLNIGRLAMRGEEPLFVPCTPKGCIELLHRYNIGIKGKRAVVIGRS 240

Query: 241 NIVGTPAALLLQREDATVSVVHSRTKDPEELTRQADIIISAVGKANMVEGSWIKPGAVII 300
           NIVGTPAALLLQ EDATVSVVHSRTKDPEELTRQADIIISAVGKANMV+GSWIKPGAVII
Sbjct: 241 NIVGTPAALLLQGEDATVSVVHSRTKDPEELTRQADIIISAVGKANMVKGSWIKPGAVII 300

Query: 301 DVGINPVEDASSPRGYRLVGDVCYEEASKIASAITPVPGGVGPMTIAMLLSNTLTAAKRK 360
           DVGINPVEDASSPRGYRLVGDVCYEEASKIASAITPVPGGVGPMTIAMLLSNTLTAAKR 
Sbjct: 301 DVGINPVEDASSPRGYRLVGDVCYEEASKIASAITPVPGGVGPMTIAMLLSNTLTAAKRT 360

Query: 361 HNFQ 365
           HNFQ
Sbjct: 361 HNFQ 363

BLAST of CmaCh06G010950 vs. ExPASy TrEMBL
Match: A0A1S3AV55 (Methenyltetrahydrofolate cyclohydrolase OS=Cucumis melo OX=3656 GN=LOC103483291 PE=3 SV=1)

HSP 1 Score: 663.7 bits (1711), Expect = 4.5e-187
Identity = 342/364 (93.96%), Postives = 351/364 (96.43%), Query Frame = 0

Query: 1   MASSMKFTDCSPSAIVRLVPSTRTRLRSFSDGAVLFHRVLGRSCPAYPRTFVSRSSISTL 60
           MASSMKFTDCSPSA VRL PS RTR RS SDGAVLFHR+L RS PA+PRTFVSRSS STL
Sbjct: 1   MASSMKFTDCSPSATVRLFPSARTRFRS-SDGAVLFHRLLDRSRPAFPRTFVSRSSASTL 60

Query: 61  PVISASISTEATAQVIDGKLVAKQIREEITAEVSRMKDAIGVVPGLAVILVGDRKDSATY 120
           PVISASIST++TA+VIDGKLVAKQIREEITAEVSRMKDAIGVVPGLAVILVGDRKDSATY
Sbjct: 61  PVISASISTQSTAEVIDGKLVAKQIREEITAEVSRMKDAIGVVPGLAVILVGDRKDSATY 120

Query: 121 VRNKKKACESVGIKSFEVNLPENATEQEVLKHISDFNDDPSVHGILVQLPLPKHMNEQNV 180
           VRNKKKACESVGIKSFEVNLPE+ TEQEVLK+ISDFNDDPSVHGILVQLPLPKHMNEQN+
Sbjct: 121 VRNKKKACESVGIKSFEVNLPEDCTEQEVLKYISDFNDDPSVHGILVQLPLPKHMNEQNI 180

Query: 181 LHAVRIEKDVDGFHPLNIGRLAMRGEEPLFVPCTPKGCIELLHRYNISIKGKRAVVIGRS 240
           LHAV IEKDVDGFHPLNIGRLAMRGEEPLFVPCTPKGCIELLHRYNI IKGKRAVVIGRS
Sbjct: 181 LHAVSIEKDVDGFHPLNIGRLAMRGEEPLFVPCTPKGCIELLHRYNIGIKGKRAVVIGRS 240

Query: 241 NIVGTPAALLLQREDATVSVVHSRTKDPEELTRQADIIISAVGKANMVEGSWIKPGAVII 300
           NIVGTPAALLLQ EDATVSVVHSRTKDPEELTRQADIIISAVGKANMV+GSWIKPGAVII
Sbjct: 241 NIVGTPAALLLQGEDATVSVVHSRTKDPEELTRQADIIISAVGKANMVKGSWIKPGAVII 300

Query: 301 DVGINPVEDASSPRGYRLVGDVCYEEASKIASAITPVPGGVGPMTIAMLLSNTLTAAKRK 360
           DVGINPVEDASSPRGYRLVGDVCYEEASKIASAITPVPGGVGPMTIAMLLSNTLTAAKR 
Sbjct: 301 DVGINPVEDASSPRGYRLVGDVCYEEASKIASAITPVPGGVGPMTIAMLLSNTLTAAKRT 360

Query: 361 HNFQ 365
           HNFQ
Sbjct: 361 HNFQ 363

BLAST of CmaCh06G010950 vs. NCBI nr
Match: XP_022975109.1 (bifunctional protein FolD 4, chloroplastic [Cucurbita maxima] >XP_022975110.1 bifunctional protein FolD 4, chloroplastic [Cucurbita maxima] >XP_022975111.1 bifunctional protein FolD 4, chloroplastic [Cucurbita maxima] >XP_022975112.1 bifunctional protein FolD 4, chloroplastic [Cucurbita maxima])

HSP 1 Score: 709.9 bits (1831), Expect = 1.1e-200
Identity = 364/364 (100.00%), Postives = 364/364 (100.00%), Query Frame = 0

Query: 1   MASSMKFTDCSPSAIVRLVPSTRTRLRSFSDGAVLFHRVLGRSCPAYPRTFVSRSSISTL 60
           MASSMKFTDCSPSAIVRLVPSTRTRLRSFSDGAVLFHRVLGRSCPAYPRTFVSRSSISTL
Sbjct: 1   MASSMKFTDCSPSAIVRLVPSTRTRLRSFSDGAVLFHRVLGRSCPAYPRTFVSRSSISTL 60

Query: 61  PVISASISTEATAQVIDGKLVAKQIREEITAEVSRMKDAIGVVPGLAVILVGDRKDSATY 120
           PVISASISTEATAQVIDGKLVAKQIREEITAEVSRMKDAIGVVPGLAVILVGDRKDSATY
Sbjct: 61  PVISASISTEATAQVIDGKLVAKQIREEITAEVSRMKDAIGVVPGLAVILVGDRKDSATY 120

Query: 121 VRNKKKACESVGIKSFEVNLPENATEQEVLKHISDFNDDPSVHGILVQLPLPKHMNEQNV 180
           VRNKKKACESVGIKSFEVNLPENATEQEVLKHISDFNDDPSVHGILVQLPLPKHMNEQNV
Sbjct: 121 VRNKKKACESVGIKSFEVNLPENATEQEVLKHISDFNDDPSVHGILVQLPLPKHMNEQNV 180

Query: 181 LHAVRIEKDVDGFHPLNIGRLAMRGEEPLFVPCTPKGCIELLHRYNISIKGKRAVVIGRS 240
           LHAVRIEKDVDGFHPLNIGRLAMRGEEPLFVPCTPKGCIELLHRYNISIKGKRAVVIGRS
Sbjct: 181 LHAVRIEKDVDGFHPLNIGRLAMRGEEPLFVPCTPKGCIELLHRYNISIKGKRAVVIGRS 240

Query: 241 NIVGTPAALLLQREDATVSVVHSRTKDPEELTRQADIIISAVGKANMVEGSWIKPGAVII 300
           NIVGTPAALLLQREDATVSVVHSRTKDPEELTRQADIIISAVGKANMVEGSWIKPGAVII
Sbjct: 241 NIVGTPAALLLQREDATVSVVHSRTKDPEELTRQADIIISAVGKANMVEGSWIKPGAVII 300

Query: 301 DVGINPVEDASSPRGYRLVGDVCYEEASKIASAITPVPGGVGPMTIAMLLSNTLTAAKRK 360
           DVGINPVEDASSPRGYRLVGDVCYEEASKIASAITPVPGGVGPMTIAMLLSNTLTAAKRK
Sbjct: 301 DVGINPVEDASSPRGYRLVGDVCYEEASKIASAITPVPGGVGPMTIAMLLSNTLTAAKRK 360

Query: 361 HNFQ 365
           HNFQ
Sbjct: 361 HNFQ 364

BLAST of CmaCh06G010950 vs. NCBI nr
Match: KAG7028621.1 (Bifunctional protein FolD 4, chloroplastic, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 696.0 bits (1795), Expect = 1.7e-196
Identity = 360/364 (98.90%), Postives = 360/364 (98.90%), Query Frame = 0

Query: 1   MASSMKFTDCSPSAIVRLVPSTRTRLRSFSDGAVLFHRVLGRSCPAYPRTFVSRSSISTL 60
           MASSMKFTDCSPSAIVRLVPSTRTRLRS SD AVLFHRVLGRSCPAYPRTFVSRSSISTL
Sbjct: 52  MASSMKFTDCSPSAIVRLVPSTRTRLRS-SDSAVLFHRVLGRSCPAYPRTFVSRSSISTL 111

Query: 61  PVISASISTEATAQVIDGKLVAKQIREEITAEVSRMKDAIGVVPGLAVILVGDRKDSATY 120
           PVISASISTEATAQVIDGKLVAKQIREEITAEVSRMKDAIGVVPGLAVILVGDRKDSATY
Sbjct: 112 PVISASISTEATAQVIDGKLVAKQIREEITAEVSRMKDAIGVVPGLAVILVGDRKDSATY 171

Query: 121 VRNKKKACESVGIKSFEVNLPENATEQEVLKHISDFNDDPSVHGILVQLPLPKHMNEQNV 180
           VRNKKKACESVGIKSFEVNLPENATEQEVLKHISDFNDDPSVHGILVQLPLPKHMNEQNV
Sbjct: 172 VRNKKKACESVGIKSFEVNLPENATEQEVLKHISDFNDDPSVHGILVQLPLPKHMNEQNV 231

Query: 181 LHAVRIEKDVDGFHPLNIGRLAMRGEEPLFVPCTPKGCIELLHRYNISIKGKRAVVIGRS 240
           LHAV IEKDVDGFHPLNIGRLAMRGEEPLFVPCTPKGCIELLHRYNISIKGKRAVVIGRS
Sbjct: 232 LHAVSIEKDVDGFHPLNIGRLAMRGEEPLFVPCTPKGCIELLHRYNISIKGKRAVVIGRS 291

Query: 241 NIVGTPAALLLQREDATVSVVHSRTKDPEELTRQADIIISAVGKANMVEGSWIKPGAVII 300
           NIVGTPAALLLQREDATVSVVHSRTKDPEELTRQADIIISAVGKANMVEGSWIKPGAVII
Sbjct: 292 NIVGTPAALLLQREDATVSVVHSRTKDPEELTRQADIIISAVGKANMVEGSWIKPGAVII 351

Query: 301 DVGINPVEDASSPRGYRLVGDVCYEEASKIASAITPVPGGVGPMTIAMLLSNTLTAAKRK 360
           DVGINPVEDASSPRGYRLVGDVCYEEASKIASAITPVPGGVGPMTIAMLLSNTLTAAKR 
Sbjct: 352 DVGINPVEDASSPRGYRLVGDVCYEEASKIASAITPVPGGVGPMTIAMLLSNTLTAAKRT 411

Query: 361 HNFQ 365
           HNFQ
Sbjct: 412 HNFQ 414

BLAST of CmaCh06G010950 vs. NCBI nr
Match: XP_022948974.1 (bifunctional protein FolD 4, chloroplastic-like [Cucurbita moschata] >XP_022948983.1 bifunctional protein FolD 4, chloroplastic-like [Cucurbita moschata] >XP_022948993.1 bifunctional protein FolD 4, chloroplastic-like [Cucurbita moschata] >XP_022949000.1 bifunctional protein FolD 4, chloroplastic-like [Cucurbita moschata])

HSP 1 Score: 696.0 bits (1795), Expect = 1.7e-196
Identity = 360/364 (98.90%), Postives = 360/364 (98.90%), Query Frame = 0

Query: 1   MASSMKFTDCSPSAIVRLVPSTRTRLRSFSDGAVLFHRVLGRSCPAYPRTFVSRSSISTL 60
           MASSMKFTDCSPSAIVRLVPSTRTRLRS SD AVLFHRVLGRSCPAYPRTFVSRSSISTL
Sbjct: 1   MASSMKFTDCSPSAIVRLVPSTRTRLRS-SDSAVLFHRVLGRSCPAYPRTFVSRSSISTL 60

Query: 61  PVISASISTEATAQVIDGKLVAKQIREEITAEVSRMKDAIGVVPGLAVILVGDRKDSATY 120
           PVISASISTEATAQVIDGKLVAKQIREEITAEVSRMKDAIGVVPGLAVILVGDRKDSATY
Sbjct: 61  PVISASISTEATAQVIDGKLVAKQIREEITAEVSRMKDAIGVVPGLAVILVGDRKDSATY 120

Query: 121 VRNKKKACESVGIKSFEVNLPENATEQEVLKHISDFNDDPSVHGILVQLPLPKHMNEQNV 180
           VRNKKKACESVGIKSFEVNLPENATEQEVLKHISDFNDDPSVHGILVQLPLPKHMNEQNV
Sbjct: 121 VRNKKKACESVGIKSFEVNLPENATEQEVLKHISDFNDDPSVHGILVQLPLPKHMNEQNV 180

Query: 181 LHAVRIEKDVDGFHPLNIGRLAMRGEEPLFVPCTPKGCIELLHRYNISIKGKRAVVIGRS 240
           LHAV IEKDVDGFHPLNIGRLAMRGEEPLFVPCTPKGCIELLHRYNISIKGKRAVVIGRS
Sbjct: 181 LHAVSIEKDVDGFHPLNIGRLAMRGEEPLFVPCTPKGCIELLHRYNISIKGKRAVVIGRS 240

Query: 241 NIVGTPAALLLQREDATVSVVHSRTKDPEELTRQADIIISAVGKANMVEGSWIKPGAVII 300
           NIVGTPAALLLQREDATVSVVHSRTKDPEELTRQADIIISAVGKANMVEGSWIKPGAVII
Sbjct: 241 NIVGTPAALLLQREDATVSVVHSRTKDPEELTRQADIIISAVGKANMVEGSWIKPGAVII 300

Query: 301 DVGINPVEDASSPRGYRLVGDVCYEEASKIASAITPVPGGVGPMTIAMLLSNTLTAAKRK 360
           DVGINPVEDASSPRGYRLVGDVCYEEASKIASAITPVPGGVGPMTIAMLLSNTLTAAKR 
Sbjct: 301 DVGINPVEDASSPRGYRLVGDVCYEEASKIASAITPVPGGVGPMTIAMLLSNTLTAAKRT 360

Query: 361 HNFQ 365
           HNFQ
Sbjct: 361 HNFQ 363

BLAST of CmaCh06G010950 vs. NCBI nr
Match: KAG6597151.1 (K(+) efflux antiporter 2, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 696.0 bits (1795), Expect = 1.7e-196
Identity = 360/364 (98.90%), Postives = 360/364 (98.90%), Query Frame = 0

Query: 1    MASSMKFTDCSPSAIVRLVPSTRTRLRSFSDGAVLFHRVLGRSCPAYPRTFVSRSSISTL 60
            MASSMKFTDCSPSAIVRLVPSTRTRLRS SD AVLFHRVLGRSCPAYPRTFVSRSSISTL
Sbjct: 1225 MASSMKFTDCSPSAIVRLVPSTRTRLRS-SDSAVLFHRVLGRSCPAYPRTFVSRSSISTL 1284

Query: 61   PVISASISTEATAQVIDGKLVAKQIREEITAEVSRMKDAIGVVPGLAVILVGDRKDSATY 120
            PVISASISTEATAQVIDGKLVAKQIREEITAEVSRMKDAIGVVPGLAVILVGDRKDSATY
Sbjct: 1285 PVISASISTEATAQVIDGKLVAKQIREEITAEVSRMKDAIGVVPGLAVILVGDRKDSATY 1344

Query: 121  VRNKKKACESVGIKSFEVNLPENATEQEVLKHISDFNDDPSVHGILVQLPLPKHMNEQNV 180
            VRNKKKACESVGIKSFEVNLPENATEQEVLKHISDFNDDPSVHGILVQLPLPKHMNEQNV
Sbjct: 1345 VRNKKKACESVGIKSFEVNLPENATEQEVLKHISDFNDDPSVHGILVQLPLPKHMNEQNV 1404

Query: 181  LHAVRIEKDVDGFHPLNIGRLAMRGEEPLFVPCTPKGCIELLHRYNISIKGKRAVVIGRS 240
            LHAV IEKDVDGFHPLNIGRLAMRGEEPLFVPCTPKGCIELLHRYNISIKGKRAVVIGRS
Sbjct: 1405 LHAVSIEKDVDGFHPLNIGRLAMRGEEPLFVPCTPKGCIELLHRYNISIKGKRAVVIGRS 1464

Query: 241  NIVGTPAALLLQREDATVSVVHSRTKDPEELTRQADIIISAVGKANMVEGSWIKPGAVII 300
            NIVGTPAALLLQREDATVSVVHSRTKDPEELTRQADIIISAVGKANMVEGSWIKPGAVII
Sbjct: 1465 NIVGTPAALLLQREDATVSVVHSRTKDPEELTRQADIIISAVGKANMVEGSWIKPGAVII 1524

Query: 301  DVGINPVEDASSPRGYRLVGDVCYEEASKIASAITPVPGGVGPMTIAMLLSNTLTAAKRK 360
            DVGINPVEDASSPRGYRLVGDVCYEEASKIASAITPVPGGVGPMTIAMLLSNTLTAAKR 
Sbjct: 1525 DVGINPVEDASSPRGYRLVGDVCYEEASKIASAITPVPGGVGPMTIAMLLSNTLTAAKRT 1584

Query: 361  HNFQ 365
            HNFQ
Sbjct: 1585 HNFQ 1587

BLAST of CmaCh06G010950 vs. NCBI nr
Match: XP_023539315.1 (bifunctional protein FolD 4, chloroplastic [Cucurbita pepo subsp. pepo] >XP_023539316.1 bifunctional protein FolD 4, chloroplastic [Cucurbita pepo subsp. pepo] >XP_023539317.1 bifunctional protein FolD 4, chloroplastic [Cucurbita pepo subsp. pepo])

HSP 1 Score: 694.9 bits (1792), Expect = 3.7e-196
Identity = 359/364 (98.63%), Postives = 360/364 (98.90%), Query Frame = 0

Query: 1   MASSMKFTDCSPSAIVRLVPSTRTRLRSFSDGAVLFHRVLGRSCPAYPRTFVSRSSISTL 60
           MASSMKFTDCSPSAIVRLVP+TRTRLRS SD AVLFHRVLGRSCPAYPRTFVSRSSISTL
Sbjct: 1   MASSMKFTDCSPSAIVRLVPTTRTRLRS-SDSAVLFHRVLGRSCPAYPRTFVSRSSISTL 60

Query: 61  PVISASISTEATAQVIDGKLVAKQIREEITAEVSRMKDAIGVVPGLAVILVGDRKDSATY 120
           PVISASISTEATAQVIDGKLVAKQIREEITAEVSRMKDAIGVVPGLAVILVGDRKDSATY
Sbjct: 61  PVISASISTEATAQVIDGKLVAKQIREEITAEVSRMKDAIGVVPGLAVILVGDRKDSATY 120

Query: 121 VRNKKKACESVGIKSFEVNLPENATEQEVLKHISDFNDDPSVHGILVQLPLPKHMNEQNV 180
           VRNKKKACESVGIKSFEVNLPENATEQEVLKHISDFNDDPSVHGILVQLPLPKHMNEQNV
Sbjct: 121 VRNKKKACESVGIKSFEVNLPENATEQEVLKHISDFNDDPSVHGILVQLPLPKHMNEQNV 180

Query: 181 LHAVRIEKDVDGFHPLNIGRLAMRGEEPLFVPCTPKGCIELLHRYNISIKGKRAVVIGRS 240
           LHAV IEKDVDGFHPLNIGRLAMRGEEPLFVPCTPKGCIELLHRYNISIKGKRAVVIGRS
Sbjct: 181 LHAVSIEKDVDGFHPLNIGRLAMRGEEPLFVPCTPKGCIELLHRYNISIKGKRAVVIGRS 240

Query: 241 NIVGTPAALLLQREDATVSVVHSRTKDPEELTRQADIIISAVGKANMVEGSWIKPGAVII 300
           NIVGTPAALLLQREDATVSVVHSRTKDPEELTRQADIIISAVGKANMVEGSWIKPGAVII
Sbjct: 241 NIVGTPAALLLQREDATVSVVHSRTKDPEELTRQADIIISAVGKANMVEGSWIKPGAVII 300

Query: 301 DVGINPVEDASSPRGYRLVGDVCYEEASKIASAITPVPGGVGPMTIAMLLSNTLTAAKRK 360
           DVGINPVEDASSPRGYRLVGDVCYEEASKIASAITPVPGGVGPMTIAMLLSNTLTAAKR 
Sbjct: 301 DVGINPVEDASSPRGYRLVGDVCYEEASKIASAITPVPGGVGPMTIAMLLSNTLTAAKRT 360

Query: 361 HNFQ 365
           HNFQ
Sbjct: 361 HNFQ 363

BLAST of CmaCh06G010950 vs. TAIR 10
Match: AT4G00620.1 (Amino acid dehydrogenase family protein )

HSP 1 Score: 528.9 bits (1361), Expect = 3.3e-150
Identity = 274/365 (75.07%), Postives = 307/365 (84.11%), Query Frame = 0

Query: 3   SSMKFTDCSPSAIVRLVPSTRTRLRSFSDGAVLFHRVLGR---SCPAYPRTFVSRSSIST 62
           +SM FTDCS +   RL+   R      S G  L  + +G+      A  R    RSS S 
Sbjct: 2   ASMMFTDCSSTTTSRLIHLNR------SSGTFLLRQCVGQLRLQTTASGRGCCIRSSSSP 61

Query: 63  LPVISASISTEATAQVIDGKLVAKQIREEITAEVSRMKDAIGVVPGLAVILVGDRKDSAT 122
           +  ISA   +E  A VIDGK VAK+IR+EIT EVSRMK++IGV+PGLAVILVGDRKDSAT
Sbjct: 62  ISSISADTKSEGGAIVIDGKAVAKKIRDEITIEVSRMKESIGVIPGLAVILVGDRKDSAT 121

Query: 123 YVRNKKKACESVGIKSFEVNLPENATEQEVLKHISDFNDDPSVHGILVQLPLPKHMNEQN 182
           YVRNKKKAC+SVGIKSFEV L E+++E+EVLK +S FNDDPSVHGILVQLPLP HM+EQN
Sbjct: 122 YVRNKKKACDSVGIKSFEVRLAEDSSEEEVLKSVSGFNDDPSVHGILVQLPLPSHMDEQN 181

Query: 183 VLHAVRIEKDVDGFHPLNIGRLAMRGEEPLFVPCTPKGCIELLHRYNISIKGKRAVVIGR 242
           +L+AV IEKDVDGFHPLNIGRLAMRG EPLFVPCTPKGCIELLHRYNI IKGKRAVVIGR
Sbjct: 182 ILNAVSIEKDVDGFHPLNIGRLAMRGREPLFVPCTPKGCIELLHRYNIEIKGKRAVVIGR 241

Query: 243 SNIVGTPAALLLQREDATVSVVHSRTKDPEELTRQADIIISAVGKANMVEGSWIKPGAVI 302
           SNIVG PAALLLQREDATVS++HSRTK+PEE+TR+ADIIISAVG+ NMV GSWIKPGAV+
Sbjct: 242 SNIVGMPAALLLQREDATVSIIHSRTKNPEEITREADIIISAVGQPNMVRGSWIKPGAVL 301

Query: 303 IDVGINPVEDASSPRGYRLVGDVCYEEASKIASAITPVPGGVGPMTIAMLLSNTLTAAKR 362
           IDVGINPVED S+ RGYRLVGD+CYEEASK+ASAITPVPGGVGPMTIAMLLSNTLT+AKR
Sbjct: 302 IDVGINPVEDPSAARGYRLVGDICYEEASKVASAITPVPGGVGPMTIAMLLSNTLTSAKR 360

Query: 363 KHNFQ 365
            HNFQ
Sbjct: 362 IHNFQ 360

BLAST of CmaCh06G010950 vs. TAIR 10
Match: AT4G00600.1 (Amino acid dehydrogenase family protein )

HSP 1 Score: 411.8 bits (1057), Expect = 5.9e-115
Identity = 225/363 (61.98%), Postives = 257/363 (70.80%), Query Frame = 0

Query: 7   FTDCSPSAIVRLVPSTRTRLRSFSDGAVLFHRVLGRSCPAYPRTFVSRSSISTLP----- 66
           FTDCS S   RL+                   +  R+    PR  VS+ S+ T       
Sbjct: 2   FTDCSSSTTSRLI------------------HLYNRNGVFLPRPSVSQFSLRTTASTWRC 61

Query: 67  VISASISTEATAQVIDGKLVAKQIREEITAEVSRMKDAIGVVPGLAVILVGDRKDSATYV 126
            +S   S+  +A VIDGK  AK+IR++I  EVSRMK++IGVVP                 
Sbjct: 62  TLSIRSSSSPSAIVIDGKAEAKKIRDDIKIEVSRMKESIGVVPA---------------- 121

Query: 127 RNKKKACESVGIKSFEVNLPENATEQEVLKHISDFNDDPSVHGILVQLPLPKHMNEQNVL 186
                               E+++E+EVLK++S FNDDPSVHG+LVQLPLP HM+EQN+L
Sbjct: 122 --------------------EDSSEEEVLKYVSGFNDDPSVHGVLVQLPLPSHMDEQNIL 181

Query: 187 HAVRIEKDVDGFHPLNIGRLAMRGEEPLFVPCTPKGCIELLHRYNISIKGKRAVVIGRSN 246
           +AV IEKDVDGFHPLNIGRLAMRG EPLFVPCTPKGCIELLHRYNI  KGKRAVVIGRSN
Sbjct: 182 NAVSIEKDVDGFHPLNIGRLAMRGREPLFVPCTPKGCIELLHRYNIEFKGKRAVVIGRSN 241

Query: 247 IVGTPAALLLQREDATVSVVHSRTKDPEELTRQADIIISAVGKANMVEGSWIKPGAVIID 306
           IVG PAALLLQ+EDATVS++HSRT +PEELTRQADI+ISAVGK NMV GSWIKPGAV+ID
Sbjct: 242 IVGMPAALLLQKEDATVSIIHSRTMNPEELTRQADILISAVGKPNMVRGSWIKPGAVLID 301

Query: 307 VGINPVEDASSPRGYRLVGDVCYEEASKIASAITPVPGGVGPMTIAMLLSNTLTAAKRKH 365
           VGI PVED S+  G RLVGD+CY EASKIASAITPVPG VGPMTIAMLLSNTLT+AKR H
Sbjct: 302 VGIKPVEDPSAAGGERLVGDICYVEASKIASAITPVPGDVGPMTIAMLLSNTLTSAKRIH 310

BLAST of CmaCh06G010950 vs. TAIR 10
Match: AT2G38660.1 (Amino acid dehydrogenase family protein )

HSP 1 Score: 403.3 bits (1035), Expect = 2.1e-112
Identity = 198/299 (66.22%), Postives = 236/299 (78.93%), Query Frame = 0

Query: 61  PVISASISTEATAQVIDGKLVAKQIREEITAEVSRMKDAIGVVPGLAVILVGDRKDSATY 120
           P    S  TE    VIDG ++A++IR +I +EV +MK A+G VPGLAV+LVG+++DS TY
Sbjct: 51  PPPPVSFETEQKTVVIDGNVIAEEIRTKIISEVGKMKKAVGKVPGLAVVLVGEQRDSQTY 110

Query: 121 VRNKKKACESVGIKSFEVNLPENATEQEVLKHISDFNDDPSVHGILVQLPLPKHMNEQNV 180
           VRNK KACE  GIKS    LPE+ TE +++  +  FN+D S+HGILVQLPLP+H+NE  +
Sbjct: 111 VRNKIKACEETGIKSVLAELPEDCTEGQIISVLRKFNEDTSIHGILVQLPLPQHLNESKI 170

Query: 181 LHAVRIEKDVDGFHPLNIGRLAMRGEEPLFVPCTPKGCIELLHRYNISIKGKRAVVIGRS 240
           L+ VR+EKDVDGFHPLN+G LAMRG EPLFV CTPKGC+ELL R  + I GK AVVIGRS
Sbjct: 171 LNMVRLEKDVDGFHPLNVGNLAMRGREPLFVSCTPKGCVELLIRTGVEIAGKNAVVIGRS 230

Query: 241 NIVGTPAALLLQREDATVSVVHSRTKDPEELTRQADIIISAVGKANMVEGSWIKPGAVII 300
           NIVG P +LLLQR DATVS VH+ TKDPE +TR+ADI+I+A G  N+V GSW+KPGAV+I
Sbjct: 231 NIVGLPMSLLLQRHDATVSTVHAFTKDPEHITRKADIVIAAAGIPNLVRGSWLKPGAVVI 290

Query: 301 DVGINPVEDASSPRGYRLVGDVCYEEASKIASAITPVPGGVGPMTIAMLLSNTLTAAKR 360
           DVG  PVED+S   GYRLVGDVCYEEA  +ASAITPVPGGVGPMTI MLL NTL AAKR
Sbjct: 291 DVGTTPVEDSSCEFGYRLVGDVCYEEALGVASAITPVPGGVGPMTITMLLCNTLEAAKR 349

BLAST of CmaCh06G010950 vs. TAIR 10
Match: AT2G38660.3 (Amino acid dehydrogenase family protein )

HSP 1 Score: 403.3 bits (1035), Expect = 2.1e-112
Identity = 198/299 (66.22%), Postives = 236/299 (78.93%), Query Frame = 0

Query: 61  PVISASISTEATAQVIDGKLVAKQIREEITAEVSRMKDAIGVVPGLAVILVGDRKDSATY 120
           P    S  TE    VIDG ++A++IR +I +EV +MK A+G VPGLAV+LVG+++DS TY
Sbjct: 51  PPPPVSFETEQKTVVIDGNVIAEEIRTKIISEVGKMKKAVGKVPGLAVVLVGEQRDSQTY 110

Query: 121 VRNKKKACESVGIKSFEVNLPENATEQEVLKHISDFNDDPSVHGILVQLPLPKHMNEQNV 180
           VRNK KACE  GIKS    LPE+ TE +++  +  FN+D S+HGILVQLPLP+H+NE  +
Sbjct: 111 VRNKIKACEETGIKSVLAELPEDCTEGQIISVLRKFNEDTSIHGILVQLPLPQHLNESKI 170

Query: 181 LHAVRIEKDVDGFHPLNIGRLAMRGEEPLFVPCTPKGCIELLHRYNISIKGKRAVVIGRS 240
           L+ VR+EKDVDGFHPLN+G LAMRG EPLFV CTPKGC+ELL R  + I GK AVVIGRS
Sbjct: 171 LNMVRLEKDVDGFHPLNVGNLAMRGREPLFVSCTPKGCVELLIRTGVEIAGKNAVVIGRS 230

Query: 241 NIVGTPAALLLQREDATVSVVHSRTKDPEELTRQADIIISAVGKANMVEGSWIKPGAVII 300
           NIVG P +LLLQR DATVS VH+ TKDPE +TR+ADI+I+A G  N+V GSW+KPGAV+I
Sbjct: 231 NIVGLPMSLLLQRHDATVSTVHAFTKDPEHITRKADIVIAAAGIPNLVRGSWLKPGAVVI 290

Query: 301 DVGINPVEDASSPRGYRLVGDVCYEEASKIASAITPVPGGVGPMTIAMLLSNTLTAAKR 360
           DVG  PVED+S   GYRLVGDVCYEEA  +ASAITPVPGGVGPMTI MLL NTL AAKR
Sbjct: 291 DVGTTPVEDSSCEFGYRLVGDVCYEEALGVASAITPVPGGVGPMTITMLLCNTLEAAKR 349

BLAST of CmaCh06G010950 vs. TAIR 10
Match: AT3G12290.1 (Amino acid dehydrogenase family protein )

HSP 1 Score: 387.9 bits (995), Expect = 9.1e-108
Identity = 184/292 (63.01%), Postives = 232/292 (79.45%), Query Frame = 0

Query: 68  STEATAQVIDGKLVAKQIREEITAEVSRMKDAIGVVPGLAVILVGDRKDSATYVRNKKKA 127
           S++ TA++IDGK +A  IR EI  EV  + +  G VPGLAV++VG RKDS TYV  K+KA
Sbjct: 4   SSDHTAKIIDGKAIAHTIRSEIAEEVRGLSEKHGKVPGLAVVIVGSRKDSQTYVNTKRKA 63

Query: 128 CESVGIKSFEVNLPENATEQEVLKHISDFNDDPSVHGILVQLPLPKHMNEQNVLHAVRIE 187
           C  VGIKSF+V LPE  +E +++  + + N +P VHGILVQLPLPKH+NE+++L A+ I+
Sbjct: 64  CAEVGIKSFDVGLPEEVSEADLISKVHELNSNPDVHGILVQLPLPKHINEEHILGAISID 123

Query: 188 KDVDGFHPLNIGRLAMRGEEPLFVPCTPKGCIELLHRYNISIKGKRAVVIGRSNIVGTPA 247
           KDVDGFHPLNIG+LAM+G EPLF+PCTPKGC+ELL R  + IKG+RAVV+GRSNIVG P 
Sbjct: 124 KDVDGFHPLNIGKLAMKGREPLFLPCTPKGCLELLARSGVKIKGQRAVVVGRSNIVGLPV 183

Query: 248 ALLLQREDATVSVVHSRTKDPEELTRQADIIISAVGKANMVEGSWIKPGAVIIDVGINPV 307
           +LLL + DATV+ VHS TKDPE + R+ADI+I+A G+A+M++G+WIKPGA +IDVG N V
Sbjct: 184 SLLLLKADATVTTVHSHTKDPEAIIREADIVIAACGQAHMIKGNWIKPGAAVIDVGTNAV 243

Query: 308 EDASSPRGYRLVGDVCYEEASKIASAITPVPGGVGPMTIAMLLSNTLTAAKR 360
            D S   GYRLVGDV + EASK+A  ITPVPGGVGPMT+AMLL NT+  AKR
Sbjct: 244 SDPSKKSGYRLVGDVDFAEASKVAGFITPVPGGVGPMTVAMLLRNTVDGAKR 295

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O652714.6e-14975.07Bifunctional protein FolD 4, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=FO... [more]
O652698.3e-11461.98Bifunctional protein FolD 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=FO... [more]
A2RVV72.9e-11166.22Bifunctional protein FolD 1, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=FO... [more]
Q9LHH71.3e-10663.01Bifunctional protein FolD 2 OS=Arabidopsis thaliana OX=3702 GN=FOLD2 PE=2 SV=1[more]
A0LE042.6e-8356.90Bifunctional protein FolD OS=Magnetococcus marinus (strain ATCC BAA-1437 / JCM 1... [more]
Match NameE-valueIdentityDescription
A0A6J1IDA15.4e-201100.00Methenyltetrahydrofolate cyclohydrolase OS=Cucurbita maxima OX=3661 GN=LOC111474... [more]
A0A6J1GAU18.1e-19798.90Methenyltetrahydrofolate cyclohydrolase OS=Cucurbita moschata OX=3662 GN=LOC1114... [more]
A0A0A0L8Y43.4e-18794.23Methenyltetrahydrofolate cyclohydrolase OS=Cucumis sativus OX=3659 GN=Csa_3G1226... [more]
A0A5A7TZL14.5e-18793.96Methenyltetrahydrofolate cyclohydrolase OS=Cucumis melo var. makuwa OX=1194695 G... [more]
A0A1S3AV554.5e-18793.96Methenyltetrahydrofolate cyclohydrolase OS=Cucumis melo OX=3656 GN=LOC103483291 ... [more]
Match NameE-valueIdentityDescription
XP_022975109.11.1e-200100.00bifunctional protein FolD 4, chloroplastic [Cucurbita maxima] >XP_022975110.1 bi... [more]
KAG7028621.11.7e-19698.90Bifunctional protein FolD 4, chloroplastic, partial [Cucurbita argyrosperma subs... [more]
XP_022948974.11.7e-19698.90bifunctional protein FolD 4, chloroplastic-like [Cucurbita moschata] >XP_0229489... [more]
KAG6597151.11.7e-19698.90K(+) efflux antiporter 2, chloroplastic, partial [Cucurbita argyrosperma subsp. ... [more]
XP_023539315.13.7e-19698.63bifunctional protein FolD 4, chloroplastic [Cucurbita pepo subsp. pepo] >XP_0235... [more]
Match NameE-valueIdentityDescription
AT4G00620.13.3e-15075.07Amino acid dehydrogenase family protein [more]
AT4G00600.15.9e-11561.98Amino acid dehydrogenase family protein [more]
AT2G38660.12.1e-11266.22Amino acid dehydrogenase family protein [more]
AT2G38660.32.1e-11266.22Amino acid dehydrogenase family protein [more]
AT3G12290.19.1e-10863.01Amino acid dehydrogenase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000672Tetrahydrofolate dehydrogenase/cyclohydrolasePRINTSPR00085THFDHDRGNASEcoord: 145..172
score: 55.22
coord: 180..201
score: 56.29
coord: 276..305
score: 55.9
coord: 317..333
score: 45.7
coord: 104..126
score: 55.69
coord: 227..247
score: 57.88
coord: 334..352
score: 81.78
IPR000672Tetrahydrofolate dehydrogenase/cyclohydrolaseHAMAPMF_01576THF_DHG_CYHcoord: 73..361
score: 39.189301
NoneNo IPR availableGENE3D3.40.50.720coord: 76..337
e-value: 1.2E-116
score: 390.5
NoneNo IPR availableGENE3D3.40.50.10860Leucine Dehydrogenase, chain A, domain 1coord: 78..355
e-value: 1.2E-116
score: 390.5
NoneNo IPR availablePANTHERPTHR48099C-1-TETRAHYDROFOLATE SYNTHASE, CYTOPLASMIC-RELATEDcoord: 51..363
NoneNo IPR availablePANTHERPTHR48099:SF10BIFUNCTIONAL PROTEIN FOLD 1, MITOCHONDRIALcoord: 51..363
NoneNo IPR availableSUPERFAMILY53223Aminoacid dehydrogenase-like, N-terminal domaincoord: 73..192
IPR020630Tetrahydrofolate dehydrogenase/cyclohydrolase, catalytic domainPFAMPF00763THF_DHG_CYHcoord: 75..191
e-value: 2.2E-42
score: 144.0
IPR020631Tetrahydrofolate dehydrogenase/cyclohydrolase, NAD(P)-binding domainPFAMPF02882THF_DHG_CYH_Ccoord: 194..360
e-value: 1.1E-69
score: 232.9
IPR020631Tetrahydrofolate dehydrogenase/cyclohydrolase, NAD(P)-binding domainCDDcd01080NAD_bind_m-THF_DH_Cyclohydcoord: 186..358
e-value: 1.05211E-99
score: 290.611
IPR020867Tetrahydrofolate dehydrogenase/cyclohydrolase, conserved sitePROSITEPS00767THF_DHG_CYH_2coord: 338..346
IPR036291NAD(P)-binding domain superfamilySUPERFAMILY51735NAD(P)-binding Rossmann-fold domainscoord: 193..359

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh06G010950.1CmaCh06G010950.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0035999 tetrahydrofolate interconversion
cellular_component GO:0005829 cytosol
molecular_function GO:0004477 methenyltetrahydrofolate cyclohydrolase activity
molecular_function GO:0004488 methylenetetrahydrofolate dehydrogenase (NADP+) activity
molecular_function GO:0003824 catalytic activity