CmaCh06G010150 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh06G010150
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Descriptionaspartyl protease family protein 2-like
LocationCma_Chr06: 6866760 .. 6868184 (+)
RNA-Seq ExpressionCmaCh06G010150
SyntenyCmaCh06G010150
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAGGCAAAAACCAGTGCATTACCCTTTATCGGCTTTCTTCTCACTCTTCTCTCTCTCTCCACCGCCTTCTCCGACTTCCAGACCCTTATACCCAAACCTCTTCCGGCTTCACCTTCACTCTTCTCGCCTGAATCCGACACCGATTCCGAGTCTTTCATCTCCTCGGAGGCCGGCTTAGAGTTGCAGCTTCACCATTTGGATGCTCTGTCTCTGAACAGAACGCCGGAGGAGCTCTTCCATCTCCGCCTTCAGAGAGACGCTCTCAGAGTCACGAAACTGAGTTCACTCAGTGGTGGCTCTCAGAATCTTAGCCAAGCTAGTGGGACCAGCCACGGGACCACTGGGTTCAGTAGCTCAGTGATCTCGGGACTCGCTCAGGGTAGCGGCGAGTACTTCACGCGCATCGGCGTTGGCACGCCGCCCAAGTATATCTACATGGTTCTTGACACTGGTAGCGATATTGTTTGGCTACAGTGCGCTCCCTGTAAGAATTGCTACTCTCAGACCGACCCGGTTTTCAACCCGGTTAAGTCCAGATCCTACTCCAAGGTCCTTTGCCGAACGCCGCTTTGTCTCCGGCTCGAATCTCCGGGGTGCAACCAGAAGCAGACGTGTCTCTACCAGGTTTCTTACGGGGACGGTTCCTATACCACTGGTGAATTCGTCACCGAAACCCTAACCTTCCGGCGGACTAAAGTGGAGCGCGTAGCCCTAGGTTGCGGCCACGATAATGAGGGCTTATTCGTTGGTGCGGCGGGGCTTTTAGGTCTCGGTCGGGGAGGATTGTCATTTCCGTCGCAAGCTGGCCGGAGTTTCAATCAGAAATTCTCCTACTGCTTGGTGGACCGATCCGCCTCTTCCAAACCGTCCTCCGTCGTCTTCGGTGACTCCGCCGTATCTAGAACCGCCCGGTTCACTCCTCTTCTCACAAACCCTAGGCTGGATACATTTTACTATGTCGAACTGTTAGGGATCAGCGTCGGAGGCACGCCTGTTTCCCGCATCTCCGCTTCACATTTCAAGCTCGATTCGAACGGAAATGGTGGAGTCATCATCGATTGCGGTACCTCCGTCACTCGATTAAACCGACCGGCGTACATAGCCTTGCGCAACGCCTTCCGTGCTGGAGCCTCGAGTTTGAAATTGGCCCCTGAGTTTTCCCTTTTCGATACTTGCTACGACTTATCCGGGAAAACGACGGTGAAGGTTCCGACGGTGGTGCTACATTTTAGAGGCGCTGACGTGTCGTTACCAGCGTCCAATTATCTTATCCCGGTCGACGACAACGGGAGGTTCTGCTTCGCCTTCGCTGGAACGACCAGTGGGCTGTCCATCATCGGCAACATTCAGCAGCAAGGATTCCGGGTCGTGTACGATTTGGCGGGTTCCCGGGTCGGATTCTCCCCTCGTGGTTGTGCCTGA

mRNA sequence

ATGGAGGCAAAAACCAGTGCATTACCCTTTATCGGCTTTCTTCTCACTCTTCTCTCTCTCTCCACCGCCTTCTCCGACTTCCAGACCCTTATACCCAAACCTCTTCCGGCTTCACCTTCACTCTTCTCGCCTGAATCCGACACCGATTCCGAGTCTTTCATCTCCTCGGAGGCCGGCTTAGAGTTGCAGCTTCACCATTTGGATGCTCTGTCTCTGAACAGAACGCCGGAGGAGCTCTTCCATCTCCGCCTTCAGAGAGACGCTCTCAGAGTCACGAAACTGAGTTCACTCAGTGGTGGCTCTCAGAATCTTAGCCAAGCTAGTGGGACCAGCCACGGGACCACTGGGTTCAGTAGCTCAGTGATCTCGGGACTCGCTCAGGGTAGCGGCGAGTACTTCACGCGCATCGGCGTTGGCACGCCGCCCAAGTATATCTACATGGTTCTTGACACTGGTAGCGATATTGTTTGGCTACAGTGCGCTCCCTGTAAGAATTGCTACTCTCAGACCGACCCGGTTTTCAACCCGGTTAAGTCCAGATCCTACTCCAAGGTCCTTTGCCGAACGCCGCTTTGTCTCCGGCTCGAATCTCCGGGGTGCAACCAGAAGCAGACGTGTCTCTACCAGGTTTCTTACGGGGACGGTTCCTATACCACTGGTGAATTCGTCACCGAAACCCTAACCTTCCGGCGGACTAAAGTGGAGCGCGTAGCCCTAGGTTGCGGCCACGATAATGAGGGCTTATTCGTTGGTGCGGCGGGGCTTTTAGGTCTCGGTCGGGGAGGATTGTCATTTCCGTCGCAAGCTGGCCGGAGTTTCAATCAGAAATTCTCCTACTGCTTGGTGGACCGATCCGCCTCTTCCAAACCGTCCTCCGTCGTCTTCGGTGACTCCGCCGTATCTAGAACCGCCCGGTTCACTCCTCTTCTCACAAACCCTAGGCTGGATACATTTTACTATGTCGAACTGTTAGGGATCAGCGTCGGAGGCACGCCTGTTTCCCGCATCTCCGCTTCACATTTCAAGCTCGATTCGAACGGAAATGGTGGAGTCATCATCGATTGCGGTACCTCCGTCACTCGATTAAACCGACCGGCGTACATAGCCTTGCGCAACGCCTTCCGTGCTGGAGCCTCGAGTTTGAAATTGGCCCCTGAGTTTTCCCTTTTCGATACTTGCTACGACTTATCCGGGAAAACGACGGTGAAGGTTCCGACGGTGGTGCTACATTTTAGAGGCGCTGACGTGTCGTTACCAGCGTCCAATTATCTTATCCCGGTCGACGACAACGGGAGGTTCTGCTTCGCCTTCGCTGGAACGACCAGTGGGCTGTCCATCATCGGCAACATTCAGCAGCAAGGATTCCGGGTCGTGTACGATTTGGCGGGTTCCCGGGTCGGATTCTCCCCTCGTGGTTGTGCCTGA

Coding sequence (CDS)

ATGGAGGCAAAAACCAGTGCATTACCCTTTATCGGCTTTCTTCTCACTCTTCTCTCTCTCTCCACCGCCTTCTCCGACTTCCAGACCCTTATACCCAAACCTCTTCCGGCTTCACCTTCACTCTTCTCGCCTGAATCCGACACCGATTCCGAGTCTTTCATCTCCTCGGAGGCCGGCTTAGAGTTGCAGCTTCACCATTTGGATGCTCTGTCTCTGAACAGAACGCCGGAGGAGCTCTTCCATCTCCGCCTTCAGAGAGACGCTCTCAGAGTCACGAAACTGAGTTCACTCAGTGGTGGCTCTCAGAATCTTAGCCAAGCTAGTGGGACCAGCCACGGGACCACTGGGTTCAGTAGCTCAGTGATCTCGGGACTCGCTCAGGGTAGCGGCGAGTACTTCACGCGCATCGGCGTTGGCACGCCGCCCAAGTATATCTACATGGTTCTTGACACTGGTAGCGATATTGTTTGGCTACAGTGCGCTCCCTGTAAGAATTGCTACTCTCAGACCGACCCGGTTTTCAACCCGGTTAAGTCCAGATCCTACTCCAAGGTCCTTTGCCGAACGCCGCTTTGTCTCCGGCTCGAATCTCCGGGGTGCAACCAGAAGCAGACGTGTCTCTACCAGGTTTCTTACGGGGACGGTTCCTATACCACTGGTGAATTCGTCACCGAAACCCTAACCTTCCGGCGGACTAAAGTGGAGCGCGTAGCCCTAGGTTGCGGCCACGATAATGAGGGCTTATTCGTTGGTGCGGCGGGGCTTTTAGGTCTCGGTCGGGGAGGATTGTCATTTCCGTCGCAAGCTGGCCGGAGTTTCAATCAGAAATTCTCCTACTGCTTGGTGGACCGATCCGCCTCTTCCAAACCGTCCTCCGTCGTCTTCGGTGACTCCGCCGTATCTAGAACCGCCCGGTTCACTCCTCTTCTCACAAACCCTAGGCTGGATACATTTTACTATGTCGAACTGTTAGGGATCAGCGTCGGAGGCACGCCTGTTTCCCGCATCTCCGCTTCACATTTCAAGCTCGATTCGAACGGAAATGGTGGAGTCATCATCGATTGCGGTACCTCCGTCACTCGATTAAACCGACCGGCGTACATAGCCTTGCGCAACGCCTTCCGTGCTGGAGCCTCGAGTTTGAAATTGGCCCCTGAGTTTTCCCTTTTCGATACTTGCTACGACTTATCCGGGAAAACGACGGTGAAGGTTCCGACGGTGGTGCTACATTTTAGAGGCGCTGACGTGTCGTTACCAGCGTCCAATTATCTTATCCCGGTCGACGACAACGGGAGGTTCTGCTTCGCCTTCGCTGGAACGACCAGTGGGCTGTCCATCATCGGCAACATTCAGCAGCAAGGATTCCGGGTCGTGTACGATTTGGCGGGTTCCCGGGTCGGATTCTCCCCTCGTGGTTGTGCCTGA

Protein sequence

MEAKTSALPFIGFLLTLLSLSTAFSDFQTLIPKPLPASPSLFSPESDTDSESFISSEAGLELQLHHLDALSLNRTPEELFHLRLQRDALRVTKLSSLSGGSQNLSQASGTSHGTTGFSSSVISGLAQGSGEYFTRIGVGTPPKYIYMVLDTGSDIVWLQCAPCKNCYSQTDPVFNPVKSRSYSKVLCRTPLCLRLESPGCNQKQTCLYQVSYGDGSYTTGEFVTETLTFRRTKVERVALGCGHDNEGLFVGAAGLLGLGRGGLSFPSQAGRSFNQKFSYCLVDRSASSKPSSVVFGDSAVSRTARFTPLLTNPRLDTFYYVELLGISVGGTPVSRISASHFKLDSNGNGGVIIDCGTSVTRLNRPAYIALRNAFRAGASSLKLAPEFSLFDTCYDLSGKTTVKVPTVVLHFRGADVSLPASNYLIPVDDNGRFCFAFAGTTSGLSIIGNIQQQGFRVVYDLAGSRVGFSPRGCA
Homology
BLAST of CmaCh06G010150 vs. ExPASy Swiss-Prot
Match: Q9LNJ3 (Aspartyl protease family protein 2 OS=Arabidopsis thaliana OX=3702 GN=APF2 PE=2 SV=1)

HSP 1 Score: 630.2 bits (1624), Expect = 1.9e-179
Identity = 328/476 (68.91%), Postives = 380/476 (79.83%), Query Frame = 0

Query: 13  FLLTLLSLSTAFSDFQTLIPK--PLP-ASPSLFSPESDTDSESFISSE----------AG 72
           F L+L S S +   FQTL P    LP ASP  F P  D+DSES + SE          + 
Sbjct: 15  FFLSLPSFS-SLPSFQTLFPNSHSLPCASPVSFQP--DSDSESLLESEFESGSDSESSSS 74

Query: 73  LELQLHHLDALSLNRTPEELFHLRLQRDALRVTKLSSLSGGSQNLSQASGTSHGTTGFSS 132
           + L L H+DALS N+TP+ELF  RLQRD+ RV  +++L+      +       G  GFSS
Sbjct: 75  ITLNLDHIDALSSNKTPDELFSSRLQRDSRRVKSIATLAAQIPGRNVTHAPRPG--GFSS 134

Query: 133 SVISGLAQGSGEYFTRIGVGTPPKYIYMVLDTGSDIVWLQCAPCKNCYSQTDPVFNPVKS 192
           SV+SGL+QGSGEYFTR+GVGTP +Y+YMVLDTGSDIVWLQCAPC+ CYSQ+DP+F+P KS
Sbjct: 135 SVVSGLSQGSGEYFTRLGVGTPARYVYMVLDTGSDIVWLQCAPCRRCYSQSDPIFDPRKS 194

Query: 193 RSYSKVLCRTPLCLRLESPGCN-QKQTCLYQVSYGDGSYTTGEFVTETLTFRRTKVERVA 252
           ++Y+ + C +P C RL+S GCN +++TCLYQVSYGDGS+T G+F TETLTFRR +V+ VA
Sbjct: 195 KTYATIPCSSPHCRRLDSAGCNTRRKTCLYQVSYGDGSFTVGDFSTETLTFRRNRVKGVA 254

Query: 253 LGCGHDNEGLFVGAAGLLGLGRGGLSFPSQAGRSFNQKFSYCLVDRSASSKPSSVVFGDS 312
           LGCGHDNEGLFVGAAGLLGLG+G LSFP Q G  FNQKFSYCLVDRSASSKPSSVVFG++
Sbjct: 255 LGCGHDNEGLFVGAAGLLGLGKGKLSFPGQTGHRFNQKFSYCLVDRSASSKPSSVVFGNA 314

Query: 313 AVSRTARFTPLLTNPRLDTFYYVELLGISVGGTPVSRISASHFKLDSNGNGGVIIDCGTS 372
           AVSR ARFTPLL+NP+LDTFYYV LLGISVGGT V  ++AS FKLD  GNGGVIID GTS
Sbjct: 315 AVSRIARFTPLLSNPKLDTFYYVGLLGISVGGTRVPGVTASLFKLDQIGNGGVIIDSGTS 374

Query: 373 VTRLNRPAYIALRNAFRAGASSLKLAPEFSLFDTCYDLSGKTTVKVPTVVLHFRGADVSL 432
           VTRL RPAYIA+R+AFR GA +LK AP+FSLFDTC+DLS    VKVPTVVLHFRGADVSL
Sbjct: 375 VTRLIRPAYIAMRDAFRVGAKTLKRAPDFSLFDTCFDLSNMNEVKVPTVVLHFRGADVSL 434

Query: 433 PASNYLIPVDDNGRFCFAFAGTTSGLSIIGNIQQQGFRVVYDLAGSRVGFSPRGCA 475
           PA+NYLIPVD NG+FCFAFAGT  GLSIIGNIQQQGFRVVYDLA SRVGF+P GCA
Sbjct: 435 PATNYLIPVDTNGKFCFAFAGTMGGLSIIGNIQQQGFRVVYDLASSRVGFAPGGCA 485

BLAST of CmaCh06G010150 vs. ExPASy Swiss-Prot
Match: Q9LS40 (Protein ASPARTIC PROTEASE IN GUARD CELL 1 OS=Arabidopsis thaliana OX=3702 GN=ASPG1 PE=1 SV=1)

HSP 1 Score: 398.7 bits (1023), Expect = 9.4e-110
Identity = 228/471 (48.41%), Postives = 300/471 (63.69%), Query Frame = 0

Query: 17  LLSLSTAFSDFQTLIP-KPLPASPSLFSPESDTDSESFISSEAGLELQLHHLDAL--SLN 76
           +L + ++    QT++   P  +S +   PES +D   F +S + L L+LH  D    S +
Sbjct: 37  VLDVVSSLQQTQTILSLDPTRSSLTTTKPESLSD-PVFFNSSSPLSLELHSRDTFVASQH 96

Query: 77  RTPEELFHLRLQRDALRVTKL-SSLSGGSQNLSQA-------SGTSHGTTGFSSSVISGL 136
           +  + L   RL+RD+ RV  + + +    + + ++         T + T   ++ V+SG 
Sbjct: 97  KDYKSLTLSRLERDSSRVAGIVAKIRFAVEGVDRSDLKPVYNEDTRYQTEDLTTPVVSGA 156

Query: 137 AQGSGEYFTRIGVGTPPKYIYMVLDTGSDIVWLQCAPCKNCYSQTDPVFNPVKSRSYSKV 196
           +QGSGEYF+RIGVGTP K +Y+VLDTGSD+ W+QC PC +CY Q+DPVFNP  S +Y  +
Sbjct: 157 SQGSGEYFSRIGVGTPAKEMYLVLDTGSDVNWIQCEPCADCYQQSDPVFNPTSSSTYKSL 216

Query: 197 LCRTPLCLRLESPGCNQKQTCLYQVSYGDGSYTTGEFVTETLTFRRT-KVERVALGCGHD 256
            C  P C  LE+  C   + CLYQVSYGDGS+T GE  T+T+TF  + K+  VALGCGHD
Sbjct: 217 TCSAPQCSLLETSACRSNK-CLYQVSYGDGSFTVGELATDTVTFGNSGKINNVALGCGHD 276

Query: 257 NEGLFVGAAGLLGLGRGGLSFPSQAGRSFNQKFSYCLVDRSASSKPSSVVFGDSAVSRTA 316
           NEGLF GAAGLLGLG G LS  +Q   +    FSYCLVDR  S K SS+ F    +    
Sbjct: 277 NEGLFTGAAGLLGLGGGVLSITNQMKAT---SFSYCLVDRD-SGKSSSLDFNSVQLGGGD 336

Query: 317 RFTPLLTNPRLDTFYYVELLGISVGGTPVSRISASHFKLDSNGNGGVIIDCGTSVTRLNR 376
              PLL N ++DTFYYV L G SVGG  V  +  + F +D++G+GGVI+DCGT+VTRL  
Sbjct: 337 ATAPLLRNKKIDTFYYVGLSGFSVGGEKVV-LPDAIFDVDASGSGGVILDCGTAVTRLQT 396

Query: 377 PAYIALRNAFRAGASSLKL-APEFSLFDTCYDLSGKTTVKVPTVVLHFRGA-DVSLPASN 436
            AY +LR+AF     +LK  +   SLFDTCYD S  +TVKVPTV  HF G   + LPA N
Sbjct: 397 QAYNSLRDAFLKLTVNLKKGSSSISLFDTCYDFSSLSTVKVPTVAFHFTGGKSLDLPAKN 456

Query: 437 YLIPVDDNGRFCFAFAGTTSGLSIIGNIQQQGFRVVYDLAGSRVGFSPRGC 474
           YLIPVDD+G FCFAFA T+S LSIIGN+QQQG R+ YDL+ + +G S   C
Sbjct: 457 YLIPVDDSGTFCFAFAPTSSSLSIIGNVQQQGTRITYDLSKNVIGLSGNKC 500

BLAST of CmaCh06G010150 vs. ExPASy Swiss-Prot
Match: Q9LHE3 (Protein ASPARTIC PROTEASE IN GUARD CELL 2 OS=Arabidopsis thaliana OX=3702 GN=ASPG2 PE=2 SV=1)

HSP 1 Score: 385.6 bits (989), Expect = 8.3e-106
Identity = 218/474 (45.99%), Postives = 291/474 (61.39%), Query Frame = 0

Query: 8   LPFIGFLLTL-----LSLSTAFSDFQTLIPKPLPASPSLFSPESDTDSESFISSEAGLEL 67
           LP   F L L      S S +F DFQ +     P + +   P+ +    S  SS +   L
Sbjct: 3   LPLFFFFLHLHLHLSSSSSISFPDFQIIDVLQPPLTVTATLPDFNNTHFSDESS-SKYTL 62

Query: 68  QLHHLDALS--LNRTPEELFHLRLQRDALRVTKLSSLSGGSQNLSQASGTSHGTTGFSSS 127
           +L H D       R      H R++RD  RV+ +  L   S  +  +S + +    F S 
Sbjct: 63  RLLHRDRFPSVTYRNHHHRLHARMRRDTDRVSAI--LRRISGKVIPSSDSRYEVNDFGSD 122

Query: 128 VISGLAQGSGEYFTRIGVGTPPKYIYMVLDTGSDIVWLQCAPCKNCYSQTDPVFNPVKSR 187
           ++SG+ QGSGEYF RIGVG+PP+  YMV+D+GSD+VW+QC PCK CY Q+DPVF+P KS 
Sbjct: 123 IVSGMDQGSGEYFVRIGVGSPPRDQYMVIDSGSDMVWVQCQPCKLCYKQSDPVFDPAKSG 182

Query: 188 SYSKVLCRTPLCLRLESPGCNQKQTCLYQVSYGDGSYTTGEFVTETLTFRRTKVERVALG 247
           SY+ V C + +C R+E+ GC+    C Y+V YGDGSYT G    ETLTF +T V  VA+G
Sbjct: 183 SYTGVSCGSSVCDRIENSGCH-SGGCRYEVMYGDGSYTKGTLALETLTFAKTVVRNVAMG 242

Query: 248 CGHDNEGLFVGAAGLLGLGRGGLSFPSQAGRSFNQKFSYCLVDRSASSKPSSVVFGDSAV 307
           CGH N G+F+GAAGLLG+G G +SF  Q        F YCLV R   S   S+VFG  A+
Sbjct: 243 CGHRNRGMFIGAAGLLGIGGGSMSFVGQLSGQTGGAFGYCLVSRGTDS-TGSLVFGREAL 302

Query: 308 SRTARFTPLLTNPRLDTFYYVELLGISVGGTPVSRISASHFKLDSNGNGGVIIDCGTSVT 367
              A + PL+ NPR  +FYYV L G+ VGG  +  +    F L   G+GGV++D GT+VT
Sbjct: 303 PVGASWVPLVRNPRAPSFYYVGLKGLGVGGVRIP-LPDGVFDLTETGDGGVVMDTGTAVT 362

Query: 368 RLNRPAYIALRNAFRAGASSLKLAPEFSLFDTCYDLSGKTTVKVPTVVLHF-RGADVSLP 427
           RL   AY+A R+ F++  ++L  A   S+FDTCYDLSG  +V+VPTV  +F  G  ++LP
Sbjct: 363 RLPTAAYVAFRDGFKSQTANLPRASGVSIFDTCYDLSGFVSVRVPTVSFYFTEGPVLTLP 422

Query: 428 ASNYLIPVDDNGRFCFAFAGTTSGLSIIGNIQQQGFRVVYDLAGSRVGFSPRGC 474
           A N+L+PVDD+G +CFAFA + +GLSIIGNIQQ+G +V +D A   VGF P  C
Sbjct: 423 ARNFLMPVDDSGTYCFAFAASPTGLSIIGNIQQEGIQVSFDGANGFVGFGPNVC 470

BLAST of CmaCh06G010150 vs. ExPASy Swiss-Prot
Match: Q8S9J6 (Aspartyl protease family protein At5g10770 OS=Arabidopsis thaliana OX=3702 GN=At5g10770 PE=2 SV=1)

HSP 1 Score: 274.6 bits (701), Expect = 2.1e-72
Identity = 174/452 (38.50%), Postives = 242/452 (53.54%), Query Frame = 0

Query: 34  PLPASPSLFSPESDTDSESF-ISSEAGLELQLHHLDALSLNRTPEELFHLRLQRDALRVT 93
           P  +S  + SP + T   S  ++   G   +L++  A S    P+ +  LRL  D  RV 
Sbjct: 43  PSSSSSCVLSPRASTTKSSLHVTHRHGTCSRLNNGKATS----PDHVEILRL--DQARVN 102

Query: 94  KLSSLSGGSQNLSQASGTSHGTTGFSSSVIS--GLAQGSGEYFTRIGVGTPPKYIYMVLD 153
            + S       LS+   T H +   S+ + +  G   GSG Y   +G+GTP   + ++ D
Sbjct: 103 SIHS------KLSKKLATDHVSESKSTDLPAKDGSTLGSGNYIVTVGLGTPKNDLSLIFD 162

Query: 154 TGSDIVWLQCAPC-KNCYSQTDPVFNPVKSRSYSKVLCRTPLCLRLES----PGCNQKQT 213
           TGSD+ W QC PC + CY Q +P+FNP KS SY  V C +  C  L S     G      
Sbjct: 163 TGSDLTWTQCQPCVRTCYDQKEPIFNPSKSTSYYNVSCSSAACGSLSSATGNAGSCSASN 222

Query: 214 CLYQVSYGDGSYTTGEFVTETLTFRRTKV-ERVALGCGHDNEGLFVGAAGLLGLGRGGLS 273
           C+Y + YGD S++ G    E  T   + V + V  GCG +N+GLF G AGLLGLGR  LS
Sbjct: 223 CIYGIQYGDQSFSVGFLAKEKFTLTNSDVFDGVYFGCGENNQGLFTGVAGLLGLGRDKLS 282

Query: 274 FPSQAGRSFNQKFSYCLVDRSASSKPSSVVFGDSAVSRTARFTPLLTNPRLDTFYYVELL 333
           FPSQ   ++N+ FSYCL   S++S    + FG + +SR+ +FTP+ T     +FY + ++
Sbjct: 283 FPSQTATAYNKIFSYCL--PSSASYTGHLTFGSAGISRSVKFTPISTITDGTSFYGLNIV 342

Query: 334 GISVGGTPVSRISASHFKLDSNGNGGVIIDCGTSVTRLNRPAYIALRNAFRAGASSLKLA 393
            I+VGG  +  I ++ F        G +ID GT +TRL   AY ALR++F+A  S     
Sbjct: 343 AITVGGQKLP-IPSTVF-----STPGALIDSGTVITRLPPKAYAALRSSFKAKMSKYPTT 402

Query: 394 PEFSLFDTCYDLSGKTTVKVPTVVLHFRGADVSLPASNYLIPVDDNGRFCFAFAGTT--S 453
              S+ DTC+DLSG  TV +P V   F G  V    S  +  V    + C AFAG +  S
Sbjct: 403 SGVSILDTCFDLSGFKTVTIPKVAFSFSGGAVVELGSKGIFYVFKISQVCLAFAGNSDDS 462

Query: 454 GLSIIGNIQQQGFRVVYDLAGSRVGFSPRGCA 475
             +I GN+QQQ   VVYD AG RVGF+P GC+
Sbjct: 463 NAAIFGNVQQQTLEVVYDGAGGRVGFAPNGCS 474

BLAST of CmaCh06G010150 vs. ExPASy Swiss-Prot
Match: Q766C3 (Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis OX=150966 GN=nep1 PE=1 SV=1)

HSP 1 Score: 274.2 bits (700), Expect = 2.7e-72
Identity = 164/420 (39.05%), Postives = 231/420 (55.00%), Query Frame = 0

Query: 59  GLELQLHHLDALSLNRTPEELFHLRLQRDALRVTKLSSLSGGSQNLSQASGTSHGTTGFS 118
           G ++ L H+D+   N T  +L    ++R             GS+ L +     +G +G  
Sbjct: 40  GFQIMLEHVDS-GKNLTKFQLLERAIER-------------GSRRLQRLEAMLNGPSGVE 99

Query: 119 SSVISGLAQGSGEYFTRIGVGTPPKYIYMVLDTGSDIVWLQCAPCKNCYSQTDPVFNPVK 178
           +SV +    G GEY   + +GTP +    ++DTGSD++W QC PC  C++Q+ P+FNP  
Sbjct: 100 TSVYA----GDGEYLMNLSIGTPAQPFSAIMDTGSDLIWTQCQPCTQCFNQSTPIFNPQG 159

Query: 179 SRSYSKVLCRTPLCLRLESPGCNQKQTCLYQVSYGDGSYTTGEFVTETLTFRRTKVERVA 238
           S S+S + C + LC  L SP C+    C Y   YGDGS T G   TETLTF    +  + 
Sbjct: 160 SSSFSTLPCSSQLCQALSSPTCS-NNFCQYTYGYGDGSETQGSMGTETLTFGSVSIPNIT 219

Query: 239 LGCGHDNEGLFVG-AAGLLGLGRGGLSFPSQAGRSFNQKFSYCLVDRSASSKPSSVVFGD 298
            GCG +N+G   G  AGL+G+GRG LS PSQ   +   KFSYC+     SS PS+++ G 
Sbjct: 220 FGCGENNQGFGQGNGAGLVGMGRGPLSLPSQLDVT---KFSYCMTP-IGSSTPSNLLLGS 279

Query: 299 SAVSRTARF--TPLLTNPRLDTFYYVELLGISVGGTPVSRISASHFKLDS-NGNGGVIID 358
            A S TA    T L+ + ++ TFYY+ L G+SVG T +  I  S F L+S NG GG+IID
Sbjct: 280 LANSVTAGSPNTTLIQSSQIPTFYYITLNGLSVGSTRLP-IDPSAFALNSNNGTGGIIID 339

Query: 359 CGTSVTRLNRPAYIALRNAFRAGASSLKLAPEFSLFDTCYDL-SGKTTVKVPTVVLHFRG 418
            GT++T     AY ++R  F +  +   +    S FD C+   S  + +++PT V+HF G
Sbjct: 340 SGTTLTYFVNNAYQSVRQEFISQINLPVVNGSSSGFDLCFQTPSDPSNLQIPTFVMHFDG 399

Query: 419 ADVSLPASNYLIPVDDNGRFCFAFAGTTSGLSIIGNIQQQGFRVVYDLAGSRVGFSPRGC 474
            D+ LP+ NY I    NG  C A   ++ G+SI GNIQQQ   VVYD   S V F+   C
Sbjct: 400 GDLELPSENYFIS-PSNGLICLAMGSSSQGMSIFGNIQQQNMLVVYDTGNSVVSFASAQC 434

BLAST of CmaCh06G010150 vs. ExPASy TrEMBL
Match: A0A6J1IFR8 (aspartyl protease family protein 2-like OS=Cucurbita maxima OX=3661 GN=LOC111474062 PE=3 SV=1)

HSP 1 Score: 941.4 bits (2432), Expect = 1.4e-270
Identity = 474/474 (100.00%), Postives = 474/474 (100.00%), Query Frame = 0

Query: 1   MEAKTSALPFIGFLLTLLSLSTAFSDFQTLIPKPLPASPSLFSPESDTDSESFISSEAGL 60
           MEAKTSALPFIGFLLTLLSLSTAFSDFQTLIPKPLPASPSLFSPESDTDSESFISSEAGL
Sbjct: 1   MEAKTSALPFIGFLLTLLSLSTAFSDFQTLIPKPLPASPSLFSPESDTDSESFISSEAGL 60

Query: 61  ELQLHHLDALSLNRTPEELFHLRLQRDALRVTKLSSLSGGSQNLSQASGTSHGTTGFSSS 120
           ELQLHHLDALSLNRTPEELFHLRLQRDALRVTKLSSLSGGSQNLSQASGTSHGTTGFSSS
Sbjct: 61  ELQLHHLDALSLNRTPEELFHLRLQRDALRVTKLSSLSGGSQNLSQASGTSHGTTGFSSS 120

Query: 121 VISGLAQGSGEYFTRIGVGTPPKYIYMVLDTGSDIVWLQCAPCKNCYSQTDPVFNPVKSR 180
           VISGLAQGSGEYFTRIGVGTPPKYIYMVLDTGSDIVWLQCAPCKNCYSQTDPVFNPVKSR
Sbjct: 121 VISGLAQGSGEYFTRIGVGTPPKYIYMVLDTGSDIVWLQCAPCKNCYSQTDPVFNPVKSR 180

Query: 181 SYSKVLCRTPLCLRLESPGCNQKQTCLYQVSYGDGSYTTGEFVTETLTFRRTKVERVALG 240
           SYSKVLCRTPLCLRLESPGCNQKQTCLYQVSYGDGSYTTGEFVTETLTFRRTKVERVALG
Sbjct: 181 SYSKVLCRTPLCLRLESPGCNQKQTCLYQVSYGDGSYTTGEFVTETLTFRRTKVERVALG 240

Query: 241 CGHDNEGLFVGAAGLLGLGRGGLSFPSQAGRSFNQKFSYCLVDRSASSKPSSVVFGDSAV 300
           CGHDNEGLFVGAAGLLGLGRGGLSFPSQAGRSFNQKFSYCLVDRSASSKPSSVVFGDSAV
Sbjct: 241 CGHDNEGLFVGAAGLLGLGRGGLSFPSQAGRSFNQKFSYCLVDRSASSKPSSVVFGDSAV 300

Query: 301 SRTARFTPLLTNPRLDTFYYVELLGISVGGTPVSRISASHFKLDSNGNGGVIIDCGTSVT 360
           SRTARFTPLLTNPRLDTFYYVELLGISVGGTPVSRISASHFKLDSNGNGGVIIDCGTSVT
Sbjct: 301 SRTARFTPLLTNPRLDTFYYVELLGISVGGTPVSRISASHFKLDSNGNGGVIIDCGTSVT 360

Query: 361 RLNRPAYIALRNAFRAGASSLKLAPEFSLFDTCYDLSGKTTVKVPTVVLHFRGADVSLPA 420
           RLNRPAYIALRNAFRAGASSLKLAPEFSLFDTCYDLSGKTTVKVPTVVLHFRGADVSLPA
Sbjct: 361 RLNRPAYIALRNAFRAGASSLKLAPEFSLFDTCYDLSGKTTVKVPTVVLHFRGADVSLPA 420

Query: 421 SNYLIPVDDNGRFCFAFAGTTSGLSIIGNIQQQGFRVVYDLAGSRVGFSPRGCA 475
           SNYLIPVDDNGRFCFAFAGTTSGLSIIGNIQQQGFRVVYDLAGSRVGFSPRGCA
Sbjct: 421 SNYLIPVDDNGRFCFAFAGTTSGLSIIGNIQQQGFRVVYDLAGSRVGFSPRGCA 474

BLAST of CmaCh06G010150 vs. ExPASy TrEMBL
Match: A0A6J1IJ02 (aspartyl protease family protein 2-like OS=Cucurbita maxima OX=3661 GN=LOC111473763 PE=3 SV=1)

HSP 1 Score: 927.9 bits (2397), Expect = 1.6e-266
Identity = 470/474 (99.16%), Postives = 471/474 (99.37%), Query Frame = 0

Query: 1   MEAKTSALPFIGFLLTLLSLSTAFSDFQTLIPKPLPASPSLFSPESDTDSESFISSEAGL 60
           MEAKTSALPFIGFLLTLLSLSTAFSDFQTLIPKPLPASPS FSPESDTDSESFISSEAGL
Sbjct: 1   MEAKTSALPFIGFLLTLLSLSTAFSDFQTLIPKPLPASPS-FSPESDTDSESFISSEAGL 60

Query: 61  ELQLHHLDALSLNRTPEELFHLRLQRDALRVTKLSSLSGGSQNLSQASGTSHGTTGFSSS 120
           ELQLHHLDALSLNRTPEELFHLRLQRDALRVTKLSSLSGGSQNLSQASGTSHGTTGFSSS
Sbjct: 61  ELQLHHLDALSLNRTPEELFHLRLQRDALRVTKLSSLSGGSQNLSQASGTSHGTTGFSSS 120

Query: 121 VISGLAQGSGEYFTRIGVGTPPKYIYMVLDTGSDIVWLQCAPCKNCYSQTDPVFNPVKSR 180
           VISGLAQGSGEYFTRIGVGTPPKYIYMVLDTGSDIVWLQCAPCKNCYSQTDPVFNPVKSR
Sbjct: 121 VISGLAQGSGEYFTRIGVGTPPKYIYMVLDTGSDIVWLQCAPCKNCYSQTDPVFNPVKSR 180

Query: 181 SYSKVLCRTPLCLRLESPGCNQKQTCLYQVSYGDGSYTTGEFVTETLTFRRTKVERVALG 240
           SYSKVLCRTPLCLRLESPGCNQKQTCLYQVSYGDGSYTTGEFVTETLTFRRTKVERVALG
Sbjct: 181 SYSKVLCRTPLCLRLESPGCNQKQTCLYQVSYGDGSYTTGEFVTETLTFRRTKVERVALG 240

Query: 241 CGHDNEGLFVGAAGLLGLGRGGLSFPSQAGRSFNQKFSYCLVDRSASSKPSSVVFGDSAV 300
           CGHDNEGLFVGAAGLLGLGRGGLSFPSQAGRSFNQKFSYCLVDRSASSKPSSVVFGDSAV
Sbjct: 241 CGHDNEGLFVGAAGLLGLGRGGLSFPSQAGRSFNQKFSYCLVDRSASSKPSSVVFGDSAV 300

Query: 301 SRTARFTPLLTNPRLDTFYYVELLGISVGGTPVSRISASHFKLDSNGNGGVIIDCGTSVT 360
           SRTARFTPLLTNPRLDTFYYVELLGISVGGTPV  ISASHFKLDSNGNGGVIIDCGTSVT
Sbjct: 301 SRTARFTPLLTNPRLDTFYYVELLGISVGGTPVFGISASHFKLDSNGNGGVIIDCGTSVT 360

Query: 361 RLNRPAYIALRNAFRAGASSLKLAPEFSLFDTCYDLSGKTTVKVPTVVLHFRGADVSLPA 420
           RLNRPAYIALR+AFRAGASSLKLAPEFSLFDTCYDLSGKTTVKVPTVVLHFRGADVSLPA
Sbjct: 361 RLNRPAYIALRDAFRAGASSLKLAPEFSLFDTCYDLSGKTTVKVPTVVLHFRGADVSLPA 420

Query: 421 SNYLIPVDDNGRFCFAFAGTTSGLSIIGNIQQQGFRVVYDLAGSRVGFSPRGCA 475
           SNYLIPVDDNGRFCFAFAGTTSGLSIIGNIQQQGFRVVYDLAGSRVGFSPRGCA
Sbjct: 421 SNYLIPVDDNGRFCFAFAGTTSGLSIIGNIQQQGFRVVYDLAGSRVGFSPRGCA 473

BLAST of CmaCh06G010150 vs. ExPASy TrEMBL
Match: A0A6J1G0Y1 (aspartyl protease family protein 2-like OS=Cucurbita moschata OX=3662 GN=LOC111449669 PE=3 SV=1)

HSP 1 Score: 924.9 bits (2389), Expect = 1.4e-265
Identity = 467/474 (98.52%), Postives = 468/474 (98.73%), Query Frame = 0

Query: 1   MEAKTSALPFIGFLLTLLSLSTAFSDFQTLIPKPLPASPSLFSPESDTDSESFISSEAGL 60
           MEAKTSA PFIGFLLTLLSLSTAFSDFQTLIPK LPASPSL SPES TDSESFISSEAGL
Sbjct: 1   MEAKTSAFPFIGFLLTLLSLSTAFSDFQTLIPKSLPASPSLLSPESATDSESFISSEAGL 60

Query: 61  ELQLHHLDALSLNRTPEELFHLRLQRDALRVTKLSSLSGGSQNLSQASGTSHGTTGFSSS 120
           ELQLHHLDALSLNRTPEELFHLRLQRDALRVTKLSSLSGGSQNLSQASGTSHGTTGFSSS
Sbjct: 61  ELQLHHLDALSLNRTPEELFHLRLQRDALRVTKLSSLSGGSQNLSQASGTSHGTTGFSSS 120

Query: 121 VISGLAQGSGEYFTRIGVGTPPKYIYMVLDTGSDIVWLQCAPCKNCYSQTDPVFNPVKSR 180
           VISGLAQGSGEYFTRIGVGTPPKYIYMVLDTGSDIVWLQCAPCKNCYSQTDPVFNPVKSR
Sbjct: 121 VISGLAQGSGEYFTRIGVGTPPKYIYMVLDTGSDIVWLQCAPCKNCYSQTDPVFNPVKSR 180

Query: 181 SYSKVLCRTPLCLRLESPGCNQKQTCLYQVSYGDGSYTTGEFVTETLTFRRTKVERVALG 240
           SYSKVLCRTPLCLRLESPGCNQKQTCLYQVSYGDGSYTTGEFVTETLTFRRTKVERVALG
Sbjct: 181 SYSKVLCRTPLCLRLESPGCNQKQTCLYQVSYGDGSYTTGEFVTETLTFRRTKVERVALG 240

Query: 241 CGHDNEGLFVGAAGLLGLGRGGLSFPSQAGRSFNQKFSYCLVDRSASSKPSSVVFGDSAV 300
           CGHDNEGLFVGAAGLLGLGRGGLSFPSQAGRSFNQKFSYCLVDRSASSKPSSVVFGDSAV
Sbjct: 241 CGHDNEGLFVGAAGLLGLGRGGLSFPSQAGRSFNQKFSYCLVDRSASSKPSSVVFGDSAV 300

Query: 301 SRTARFTPLLTNPRLDTFYYVELLGISVGGTPVSRISASHFKLDSNGNGGVIIDCGTSVT 360
           SRTARFTPLLTNPRLDTFYYVELLGISVGGTPVS ISASHFKLD NGNGGVIIDCGTSVT
Sbjct: 301 SRTARFTPLLTNPRLDTFYYVELLGISVGGTPVSGISASHFKLDPNGNGGVIIDCGTSVT 360

Query: 361 RLNRPAYIALRNAFRAGASSLKLAPEFSLFDTCYDLSGKTTVKVPTVVLHFRGADVSLPA 420
           RLNRPAYIALR+AFRAGASSLKLAPEFSLFDTCYDLSGKTTVKVPTVVLHFRGADVSLPA
Sbjct: 361 RLNRPAYIALRDAFRAGASSLKLAPEFSLFDTCYDLSGKTTVKVPTVVLHFRGADVSLPA 420

Query: 421 SNYLIPVDDNGRFCFAFAGTTSGLSIIGNIQQQGFRVVYDLAGSRVGFSPRGCA 475
           SNYLIPVDDNGRFCFAFAGTTSGLSIIGNIQQQGFRVVYDLAGSRVGFSPRGCA
Sbjct: 421 SNYLIPVDDNGRFCFAFAGTTSGLSIIGNIQQQGFRVVYDLAGSRVGFSPRGCA 474

BLAST of CmaCh06G010150 vs. ExPASy TrEMBL
Match: A0A6J1EFG4 (aspartyl protease family protein 2-like OS=Cucurbita moschata OX=3662 GN=LOC111432036 PE=3 SV=1)

HSP 1 Score: 822.4 bits (2123), Expect = 9.8e-235
Identity = 420/479 (87.68%), Postives = 439/479 (91.65%), Query Frame = 0

Query: 1   MEAKTSALPFIGFLLTLLSLSTAFSDFQTLIPKPLPASPSLFSPESDTDSESFISSEA-- 60
           M AKTS   FI  LLTLLSLSTAFSDFQTL+P+PLP SPS  +PES+ DS+SF SSEA  
Sbjct: 1   MVAKTSPFTFIFVLLTLLSLSTAFSDFQTLVPRPLPTSPSSLAPESNEDSDSFFSSEATE 60

Query: 61  ---GLELQLHHLDALSLNRTPEELFHLRLQRDALRVTKLSSLSGGSQNLSQASGTSHGTT 120
              GL L LHHLD+LSL+RTPEELFHLRLQRDALRV KLS L+  S N+S+ASG     T
Sbjct: 61  SEPGLALHLHHLDSLSLSRTPEELFHLRLQRDALRVNKLSLLAAVSPNVSRASG-----T 120

Query: 121 GFSSSVISGLAQGSGEYFTRIGVGTPPKYIYMVLDTGSDIVWLQCAPCKNCYSQTDPVFN 180
           GFSSSVISGLAQGSGEYFTRIGVGTPP+Y+YMVLDTGSDIVWLQCAPCKNCYSQTDPVF+
Sbjct: 121 GFSSSVISGLAQGSGEYFTRIGVGTPPRYVYMVLDTGSDIVWLQCAPCKNCYSQTDPVFD 180

Query: 181 PVKSRSYSKVLCRTPLCLRLESPGCNQKQTCLYQVSYGDGSYTTGEFVTETLTFRRTKVE 240
           PVKS S+SKVLCRTPLC RLESPGCNQ+QTCLYQVSYGDGSYTTGEFVTETLTFRRTKVE
Sbjct: 181 PVKSGSFSKVLCRTPLCGRLESPGCNQRQTCLYQVSYGDGSYTTGEFVTETLTFRRTKVE 240

Query: 241 RVALGCGHDNEGLFVGAAGLLGLGRGGLSFPSQAGRSFNQKFSYCLVDRSASSKPSSVVF 300
           RVALGCGHDNEGLFVGAAGLLGLGRGGLSFPSQ GR+FNQKFSYCLVDRSASSKPSSVVF
Sbjct: 241 RVALGCGHDNEGLFVGAAGLLGLGRGGLSFPSQTGRTFNQKFSYCLVDRSASSKPSSVVF 300

Query: 301 GDSAVSRTARFTPLLTNPRLDTFYYVELLGISVGGTPVSRISASHFKLDSNGNGGVIIDC 360
           G+SAVSRTARFTPLLTNPRLDTFYYVELLGISVGG PVS IS  HFKLDS GNGGVIIDC
Sbjct: 301 GNSAVSRTARFTPLLTNPRLDTFYYVELLGISVGGRPVSGISPLHFKLDSTGNGGVIIDC 360

Query: 361 GTSVTRLNRPAYIALRNAFRAGASSLKLAPEFSLFDTCYDLSGKTTVKVPTVVLHFRGAD 420
           GTSVTRLNRPAYIALR+AFRAGASSLK A EFSLFDTCYDLSGKTTVKVPTVVLHFR AD
Sbjct: 361 GTSVTRLNRPAYIALRDAFRAGASSLKSAAEFSLFDTCYDLSGKTTVKVPTVVLHFRNAD 420

Query: 421 VSLPASNYLIPVDDNGRFCFAFAGTTSGLSIIGNIQQQGFRVVYDLAGSRVGFSPRGCA 475
           VSLPASNYLIPVD +GRFCFAFAGTTSGLSIIGNIQQQGFRVVYDLAGSRVGFSPRGCA
Sbjct: 421 VSLPASNYLIPVDGSGRFCFAFAGTTSGLSIIGNIQQQGFRVVYDLAGSRVGFSPRGCA 474

BLAST of CmaCh06G010150 vs. ExPASy TrEMBL
Match: A0A6J1IY26 (aspartyl protease family protein 2-like OS=Cucurbita maxima OX=3661 GN=LOC111479557 PE=3 SV=1)

HSP 1 Score: 822.0 bits (2122), Expect = 1.3e-234
Identity = 418/478 (87.45%), Postives = 438/478 (91.63%), Query Frame = 0

Query: 1   MEAKTSALPFIGFLLTLLSLSTAFSDFQTLIPKPLPASPSLFSPESDTDSESFIS----S 60
           M AKTS  PFI FLLTLL LSTAFSDFQTL+P+PLP SPS  +PES   S+SF S    S
Sbjct: 1   MVAKTSPFPFIFFLLTLLPLSTAFSDFQTLVPRPLPTSPSFLAPESTEGSDSFSSEATES 60

Query: 61  EAGLELQLHHLDALSLNRTPEELFHLRLQRDALRVTKLSSLSGGSQNLSQASGTSHGTTG 120
           E GL L LHHLD+LSL+RTPEELFHLRLQRDALRV KLS L+  S+N+S+ASG     TG
Sbjct: 61  EPGLALHLHHLDSLSLSRTPEELFHLRLQRDALRVNKLSLLAAASRNVSRASG-----TG 120

Query: 121 FSSSVISGLAQGSGEYFTRIGVGTPPKYIYMVLDTGSDIVWLQCAPCKNCYSQTDPVFNP 180
           FSSSVISGLAQGSGEYFTRIGVGTPP+Y+Y+VLDTGSDIVWLQCAPCKNCYSQTDPVF+P
Sbjct: 121 FSSSVISGLAQGSGEYFTRIGVGTPPRYVYLVLDTGSDIVWLQCAPCKNCYSQTDPVFDP 180

Query: 181 VKSRSYSKVLCRTPLCLRLESPGCNQKQTCLYQVSYGDGSYTTGEFVTETLTFRRTKVER 240
           VKS S+SKVLCRTPLC RLESPGCNQ+QTCLYQVSYGDGSYTTGEFVTETLTFRRTKVER
Sbjct: 181 VKSGSFSKVLCRTPLCGRLESPGCNQRQTCLYQVSYGDGSYTTGEFVTETLTFRRTKVER 240

Query: 241 VALGCGHDNEGLFVGAAGLLGLGRGGLSFPSQAGRSFNQKFSYCLVDRSASSKPSSVVFG 300
           VALGCGHDNEGLFVGAAGLLGLGRGGLSFPSQ GR+FNQKFSYCLVDRSASSKPSSVVFG
Sbjct: 241 VALGCGHDNEGLFVGAAGLLGLGRGGLSFPSQTGRAFNQKFSYCLVDRSASSKPSSVVFG 300

Query: 301 DSAVSRTARFTPLLTNPRLDTFYYVELLGISVGGTPVSRISASHFKLDSNGNGGVIIDCG 360
           +SAVSRTARFTPLLTNPRLDTFYYVELLGISVGG PVS IS  HFKLDS GNGGVIIDCG
Sbjct: 301 NSAVSRTARFTPLLTNPRLDTFYYVELLGISVGGRPVSGISPLHFKLDSTGNGGVIIDCG 360

Query: 361 TSVTRLNRPAYIALRNAFRAGASSLKLAPEFSLFDTCYDLSGKTTVKVPTVVLHFRGADV 420
           TSVTRLNRPAYIALR+AFRAGASSLK A EFSLFDTCYDLSGKTTVKVPTVVLHFR ADV
Sbjct: 361 TSVTRLNRPAYIALRDAFRAGASSLKSAAEFSLFDTCYDLSGKTTVKVPTVVLHFRNADV 420

Query: 421 SLPASNYLIPVDDNGRFCFAFAGTTSGLSIIGNIQQQGFRVVYDLAGSRVGFSPRGCA 475
           SLPASNYLIPVD +GRFCFAFAGTTSGLSIIGNIQQQGFRVVYDLAGSRVGFSPRGCA
Sbjct: 421 SLPASNYLIPVDGSGRFCFAFAGTTSGLSIIGNIQQQGFRVVYDLAGSRVGFSPRGCA 473

BLAST of CmaCh06G010150 vs. NCBI nr
Match: XP_022975090.1 (aspartyl protease family protein 2-like [Cucurbita maxima])

HSP 1 Score: 941.4 bits (2432), Expect = 3.0e-270
Identity = 474/474 (100.00%), Postives = 474/474 (100.00%), Query Frame = 0

Query: 1   MEAKTSALPFIGFLLTLLSLSTAFSDFQTLIPKPLPASPSLFSPESDTDSESFISSEAGL 60
           MEAKTSALPFIGFLLTLLSLSTAFSDFQTLIPKPLPASPSLFSPESDTDSESFISSEAGL
Sbjct: 1   MEAKTSALPFIGFLLTLLSLSTAFSDFQTLIPKPLPASPSLFSPESDTDSESFISSEAGL 60

Query: 61  ELQLHHLDALSLNRTPEELFHLRLQRDALRVTKLSSLSGGSQNLSQASGTSHGTTGFSSS 120
           ELQLHHLDALSLNRTPEELFHLRLQRDALRVTKLSSLSGGSQNLSQASGTSHGTTGFSSS
Sbjct: 61  ELQLHHLDALSLNRTPEELFHLRLQRDALRVTKLSSLSGGSQNLSQASGTSHGTTGFSSS 120

Query: 121 VISGLAQGSGEYFTRIGVGTPPKYIYMVLDTGSDIVWLQCAPCKNCYSQTDPVFNPVKSR 180
           VISGLAQGSGEYFTRIGVGTPPKYIYMVLDTGSDIVWLQCAPCKNCYSQTDPVFNPVKSR
Sbjct: 121 VISGLAQGSGEYFTRIGVGTPPKYIYMVLDTGSDIVWLQCAPCKNCYSQTDPVFNPVKSR 180

Query: 181 SYSKVLCRTPLCLRLESPGCNQKQTCLYQVSYGDGSYTTGEFVTETLTFRRTKVERVALG 240
           SYSKVLCRTPLCLRLESPGCNQKQTCLYQVSYGDGSYTTGEFVTETLTFRRTKVERVALG
Sbjct: 181 SYSKVLCRTPLCLRLESPGCNQKQTCLYQVSYGDGSYTTGEFVTETLTFRRTKVERVALG 240

Query: 241 CGHDNEGLFVGAAGLLGLGRGGLSFPSQAGRSFNQKFSYCLVDRSASSKPSSVVFGDSAV 300
           CGHDNEGLFVGAAGLLGLGRGGLSFPSQAGRSFNQKFSYCLVDRSASSKPSSVVFGDSAV
Sbjct: 241 CGHDNEGLFVGAAGLLGLGRGGLSFPSQAGRSFNQKFSYCLVDRSASSKPSSVVFGDSAV 300

Query: 301 SRTARFTPLLTNPRLDTFYYVELLGISVGGTPVSRISASHFKLDSNGNGGVIIDCGTSVT 360
           SRTARFTPLLTNPRLDTFYYVELLGISVGGTPVSRISASHFKLDSNGNGGVIIDCGTSVT
Sbjct: 301 SRTARFTPLLTNPRLDTFYYVELLGISVGGTPVSRISASHFKLDSNGNGGVIIDCGTSVT 360

Query: 361 RLNRPAYIALRNAFRAGASSLKLAPEFSLFDTCYDLSGKTTVKVPTVVLHFRGADVSLPA 420
           RLNRPAYIALRNAFRAGASSLKLAPEFSLFDTCYDLSGKTTVKVPTVVLHFRGADVSLPA
Sbjct: 361 RLNRPAYIALRNAFRAGASSLKLAPEFSLFDTCYDLSGKTTVKVPTVVLHFRGADVSLPA 420

Query: 421 SNYLIPVDDNGRFCFAFAGTTSGLSIIGNIQQQGFRVVYDLAGSRVGFSPRGCA 475
           SNYLIPVDDNGRFCFAFAGTTSGLSIIGNIQQQGFRVVYDLAGSRVGFSPRGCA
Sbjct: 421 SNYLIPVDDNGRFCFAFAGTTSGLSIIGNIQQQGFRVVYDLAGSRVGFSPRGCA 474

BLAST of CmaCh06G010150 vs. NCBI nr
Match: XP_022974939.1 (aspartyl protease family protein 2-like [Cucurbita maxima])

HSP 1 Score: 927.9 bits (2397), Expect = 3.4e-266
Identity = 470/474 (99.16%), Postives = 471/474 (99.37%), Query Frame = 0

Query: 1   MEAKTSALPFIGFLLTLLSLSTAFSDFQTLIPKPLPASPSLFSPESDTDSESFISSEAGL 60
           MEAKTSALPFIGFLLTLLSLSTAFSDFQTLIPKPLPASPS FSPESDTDSESFISSEAGL
Sbjct: 1   MEAKTSALPFIGFLLTLLSLSTAFSDFQTLIPKPLPASPS-FSPESDTDSESFISSEAGL 60

Query: 61  ELQLHHLDALSLNRTPEELFHLRLQRDALRVTKLSSLSGGSQNLSQASGTSHGTTGFSSS 120
           ELQLHHLDALSLNRTPEELFHLRLQRDALRVTKLSSLSGGSQNLSQASGTSHGTTGFSSS
Sbjct: 61  ELQLHHLDALSLNRTPEELFHLRLQRDALRVTKLSSLSGGSQNLSQASGTSHGTTGFSSS 120

Query: 121 VISGLAQGSGEYFTRIGVGTPPKYIYMVLDTGSDIVWLQCAPCKNCYSQTDPVFNPVKSR 180
           VISGLAQGSGEYFTRIGVGTPPKYIYMVLDTGSDIVWLQCAPCKNCYSQTDPVFNPVKSR
Sbjct: 121 VISGLAQGSGEYFTRIGVGTPPKYIYMVLDTGSDIVWLQCAPCKNCYSQTDPVFNPVKSR 180

Query: 181 SYSKVLCRTPLCLRLESPGCNQKQTCLYQVSYGDGSYTTGEFVTETLTFRRTKVERVALG 240
           SYSKVLCRTPLCLRLESPGCNQKQTCLYQVSYGDGSYTTGEFVTETLTFRRTKVERVALG
Sbjct: 181 SYSKVLCRTPLCLRLESPGCNQKQTCLYQVSYGDGSYTTGEFVTETLTFRRTKVERVALG 240

Query: 241 CGHDNEGLFVGAAGLLGLGRGGLSFPSQAGRSFNQKFSYCLVDRSASSKPSSVVFGDSAV 300
           CGHDNEGLFVGAAGLLGLGRGGLSFPSQAGRSFNQKFSYCLVDRSASSKPSSVVFGDSAV
Sbjct: 241 CGHDNEGLFVGAAGLLGLGRGGLSFPSQAGRSFNQKFSYCLVDRSASSKPSSVVFGDSAV 300

Query: 301 SRTARFTPLLTNPRLDTFYYVELLGISVGGTPVSRISASHFKLDSNGNGGVIIDCGTSVT 360
           SRTARFTPLLTNPRLDTFYYVELLGISVGGTPV  ISASHFKLDSNGNGGVIIDCGTSVT
Sbjct: 301 SRTARFTPLLTNPRLDTFYYVELLGISVGGTPVFGISASHFKLDSNGNGGVIIDCGTSVT 360

Query: 361 RLNRPAYIALRNAFRAGASSLKLAPEFSLFDTCYDLSGKTTVKVPTVVLHFRGADVSLPA 420
           RLNRPAYIALR+AFRAGASSLKLAPEFSLFDTCYDLSGKTTVKVPTVVLHFRGADVSLPA
Sbjct: 361 RLNRPAYIALRDAFRAGASSLKLAPEFSLFDTCYDLSGKTTVKVPTVVLHFRGADVSLPA 420

Query: 421 SNYLIPVDDNGRFCFAFAGTTSGLSIIGNIQQQGFRVVYDLAGSRVGFSPRGCA 475
           SNYLIPVDDNGRFCFAFAGTTSGLSIIGNIQQQGFRVVYDLAGSRVGFSPRGCA
Sbjct: 421 SNYLIPVDDNGRFCFAFAGTTSGLSIIGNIQQQGFRVVYDLAGSRVGFSPRGCA 473

BLAST of CmaCh06G010150 vs. NCBI nr
Match: XP_023538763.1 (aspartyl protease family protein 2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 925.6 bits (2391), Expect = 1.7e-265
Identity = 465/474 (98.10%), Postives = 469/474 (98.95%), Query Frame = 0

Query: 1   MEAKTSALPFIGFLLTLLSLSTAFSDFQTLIPKPLPASPSLFSPESDTDSESFISSEAGL 60
           MEAKTSA PFIGFLLTLLSLSTAFSDFQTL+PKPLP SPSLFSPES TDSESFISSEAGL
Sbjct: 1   MEAKTSAFPFIGFLLTLLSLSTAFSDFQTLVPKPLPPSPSLFSPESATDSESFISSEAGL 60

Query: 61  ELQLHHLDALSLNRTPEELFHLRLQRDALRVTKLSSLSGGSQNLSQASGTSHGTTGFSSS 120
           ELQLHHLDALSLNRTPEELFHLRLQRDALRVTKLSSL GGSQNL+QASGTSHGTTGFSSS
Sbjct: 61  ELQLHHLDALSLNRTPEELFHLRLQRDALRVTKLSSLRGGSQNLTQASGTSHGTTGFSSS 120

Query: 121 VISGLAQGSGEYFTRIGVGTPPKYIYMVLDTGSDIVWLQCAPCKNCYSQTDPVFNPVKSR 180
           VISGLAQGSGEYFTRIGVGTPPKYIYMVLDTGSDIVWLQCAPCKNCYSQTDPVFNPVKSR
Sbjct: 121 VISGLAQGSGEYFTRIGVGTPPKYIYMVLDTGSDIVWLQCAPCKNCYSQTDPVFNPVKSR 180

Query: 181 SYSKVLCRTPLCLRLESPGCNQKQTCLYQVSYGDGSYTTGEFVTETLTFRRTKVERVALG 240
           SYSKVLCRTPLCLRLESPGCNQ+QTCLYQVSYGDGSYTTGEFVTETLTFRRTKVERVALG
Sbjct: 181 SYSKVLCRTPLCLRLESPGCNQRQTCLYQVSYGDGSYTTGEFVTETLTFRRTKVERVALG 240

Query: 241 CGHDNEGLFVGAAGLLGLGRGGLSFPSQAGRSFNQKFSYCLVDRSASSKPSSVVFGDSAV 300
           CGHDNEGLFVGAAGLLGLGRGGLSFPSQAGRSFNQKFSYCLVDRSASSKPSSVVFGDSAV
Sbjct: 241 CGHDNEGLFVGAAGLLGLGRGGLSFPSQAGRSFNQKFSYCLVDRSASSKPSSVVFGDSAV 300

Query: 301 SRTARFTPLLTNPRLDTFYYVELLGISVGGTPVSRISASHFKLDSNGNGGVIIDCGTSVT 360
           SRTARFTPLLTNPRLDTFYYVELLGISVGGTPVS ISASHFKLDSNGNGGVIIDCGTSVT
Sbjct: 301 SRTARFTPLLTNPRLDTFYYVELLGISVGGTPVSGISASHFKLDSNGNGGVIIDCGTSVT 360

Query: 361 RLNRPAYIALRNAFRAGASSLKLAPEFSLFDTCYDLSGKTTVKVPTVVLHFRGADVSLPA 420
           RLNRPAYIALR+AFRAGASSLKLAPEFSLFDTCYDLSGKTTVKVPTVVLHFRGADVSLPA
Sbjct: 361 RLNRPAYIALRDAFRAGASSLKLAPEFSLFDTCYDLSGKTTVKVPTVVLHFRGADVSLPA 420

Query: 421 SNYLIPVDDNGRFCFAFAGTTSGLSIIGNIQQQGFRVVYDLAGSRVGFSPRGCA 475
           SNYLIPVDDNGRFCFAFAGTTSGLSIIGNIQQQGFRVVYDLAGSRVGFSPRGCA
Sbjct: 421 SNYLIPVDDNGRFCFAFAGTTSGLSIIGNIQQQGFRVVYDLAGSRVGFSPRGCA 474

BLAST of CmaCh06G010150 vs. NCBI nr
Match: XP_022945440.1 (aspartyl protease family protein 2-like [Cucurbita moschata])

HSP 1 Score: 924.9 bits (2389), Expect = 2.9e-265
Identity = 467/474 (98.52%), Postives = 468/474 (98.73%), Query Frame = 0

Query: 1   MEAKTSALPFIGFLLTLLSLSTAFSDFQTLIPKPLPASPSLFSPESDTDSESFISSEAGL 60
           MEAKTSA PFIGFLLTLLSLSTAFSDFQTLIPK LPASPSL SPES TDSESFISSEAGL
Sbjct: 1   MEAKTSAFPFIGFLLTLLSLSTAFSDFQTLIPKSLPASPSLLSPESATDSESFISSEAGL 60

Query: 61  ELQLHHLDALSLNRTPEELFHLRLQRDALRVTKLSSLSGGSQNLSQASGTSHGTTGFSSS 120
           ELQLHHLDALSLNRTPEELFHLRLQRDALRVTKLSSLSGGSQNLSQASGTSHGTTGFSSS
Sbjct: 61  ELQLHHLDALSLNRTPEELFHLRLQRDALRVTKLSSLSGGSQNLSQASGTSHGTTGFSSS 120

Query: 121 VISGLAQGSGEYFTRIGVGTPPKYIYMVLDTGSDIVWLQCAPCKNCYSQTDPVFNPVKSR 180
           VISGLAQGSGEYFTRIGVGTPPKYIYMVLDTGSDIVWLQCAPCKNCYSQTDPVFNPVKSR
Sbjct: 121 VISGLAQGSGEYFTRIGVGTPPKYIYMVLDTGSDIVWLQCAPCKNCYSQTDPVFNPVKSR 180

Query: 181 SYSKVLCRTPLCLRLESPGCNQKQTCLYQVSYGDGSYTTGEFVTETLTFRRTKVERVALG 240
           SYSKVLCRTPLCLRLESPGCNQKQTCLYQVSYGDGSYTTGEFVTETLTFRRTKVERVALG
Sbjct: 181 SYSKVLCRTPLCLRLESPGCNQKQTCLYQVSYGDGSYTTGEFVTETLTFRRTKVERVALG 240

Query: 241 CGHDNEGLFVGAAGLLGLGRGGLSFPSQAGRSFNQKFSYCLVDRSASSKPSSVVFGDSAV 300
           CGHDNEGLFVGAAGLLGLGRGGLSFPSQAGRSFNQKFSYCLVDRSASSKPSSVVFGDSAV
Sbjct: 241 CGHDNEGLFVGAAGLLGLGRGGLSFPSQAGRSFNQKFSYCLVDRSASSKPSSVVFGDSAV 300

Query: 301 SRTARFTPLLTNPRLDTFYYVELLGISVGGTPVSRISASHFKLDSNGNGGVIIDCGTSVT 360
           SRTARFTPLLTNPRLDTFYYVELLGISVGGTPVS ISASHFKLD NGNGGVIIDCGTSVT
Sbjct: 301 SRTARFTPLLTNPRLDTFYYVELLGISVGGTPVSGISASHFKLDPNGNGGVIIDCGTSVT 360

Query: 361 RLNRPAYIALRNAFRAGASSLKLAPEFSLFDTCYDLSGKTTVKVPTVVLHFRGADVSLPA 420
           RLNRPAYIALR+AFRAGASSLKLAPEFSLFDTCYDLSGKTTVKVPTVVLHFRGADVSLPA
Sbjct: 361 RLNRPAYIALRDAFRAGASSLKLAPEFSLFDTCYDLSGKTTVKVPTVVLHFRGADVSLPA 420

Query: 421 SNYLIPVDDNGRFCFAFAGTTSGLSIIGNIQQQGFRVVYDLAGSRVGFSPRGCA 475
           SNYLIPVDDNGRFCFAFAGTTSGLSIIGNIQQQGFRVVYDLAGSRVGFSPRGCA
Sbjct: 421 SNYLIPVDDNGRFCFAFAGTTSGLSIIGNIQQQGFRVVYDLAGSRVGFSPRGCA 474

BLAST of CmaCh06G010150 vs. NCBI nr
Match: KAG6597078.1 (Aspartyl protease family protein 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 914.1 bits (2361), Expect = 5.1e-262
Identity = 463/474 (97.68%), Postives = 464/474 (97.89%), Query Frame = 0

Query: 1   MEAKTSALPFIGFLLTLLSLSTAFSDFQTLIPKPLPASPSLFSPESDTDSESFISSEAGL 60
           MEAKTSA PFIG LLTLLSLS AFSDFQTLIPK LPAS SL SPES TDSE FISSEAGL
Sbjct: 1   MEAKTSAFPFIGSLLTLLSLSAAFSDFQTLIPKSLPASHSLLSPESATDSEYFISSEAGL 60

Query: 61  ELQLHHLDALSLNRTPEELFHLRLQRDALRVTKLSSLSGGSQNLSQASGTSHGTTGFSSS 120
           ELQLHHLDALSLNRTPEELFHLRLQRDALRVTKLSSLSGGSQNLSQASGTSHGTTGFSSS
Sbjct: 61  ELQLHHLDALSLNRTPEELFHLRLQRDALRVTKLSSLSGGSQNLSQASGTSHGTTGFSSS 120

Query: 121 VISGLAQGSGEYFTRIGVGTPPKYIYMVLDTGSDIVWLQCAPCKNCYSQTDPVFNPVKSR 180
           VISGLAQGSGEYFTRIGVGTPPKYIYMVLDTGSDIVWLQCAPCKNCYSQTDPVFNPVKSR
Sbjct: 121 VISGLAQGSGEYFTRIGVGTPPKYIYMVLDTGSDIVWLQCAPCKNCYSQTDPVFNPVKSR 180

Query: 181 SYSKVLCRTPLCLRLESPGCNQKQTCLYQVSYGDGSYTTGEFVTETLTFRRTKVERVALG 240
           SYSKVLCRTPLCLRLESPGCNQKQTCLYQVSYGDGSYTTGEFVTETLTFRRTKVERVALG
Sbjct: 181 SYSKVLCRTPLCLRLESPGCNQKQTCLYQVSYGDGSYTTGEFVTETLTFRRTKVERVALG 240

Query: 241 CGHDNEGLFVGAAGLLGLGRGGLSFPSQAGRSFNQKFSYCLVDRSASSKPSSVVFGDSAV 300
           CGHDNEGLFVGAAGLLGLGRGGLSFPSQAGRSFNQKFSYCLVDRSASSKPSSVVFGDSAV
Sbjct: 241 CGHDNEGLFVGAAGLLGLGRGGLSFPSQAGRSFNQKFSYCLVDRSASSKPSSVVFGDSAV 300

Query: 301 SRTARFTPLLTNPRLDTFYYVELLGISVGGTPVSRISASHFKLDSNGNGGVIIDCGTSVT 360
           SRTARFTPLLTNPRLDTFYYVELLGISVGGTPVS ISASHFKLD NGNGGVIIDCGTSVT
Sbjct: 301 SRTARFTPLLTNPRLDTFYYVELLGISVGGTPVSGISASHFKLDPNGNGGVIIDCGTSVT 360

Query: 361 RLNRPAYIALRNAFRAGASSLKLAPEFSLFDTCYDLSGKTTVKVPTVVLHFRGADVSLPA 420
           RLNRPAYIALR+AFRAGASSLKLAPEFSLFDTCYDLSGKTTVKVPTVVLHFRGADVSLPA
Sbjct: 361 RLNRPAYIALRDAFRAGASSLKLAPEFSLFDTCYDLSGKTTVKVPTVVLHFRGADVSLPA 420

Query: 421 SNYLIPVDDNGRFCFAFAGTTSGLSIIGNIQQQGFRVVYDLAGSRVGFSPRGCA 475
           SNYLIPVDDNGRFCFAFAGTTSGLSIIGNIQQQGFRVVYDLAGSRVGFSPRGCA
Sbjct: 421 SNYLIPVDDNGRFCFAFAGTTSGLSIIGNIQQQGFRVVYDLAGSRVGFSPRGCA 474

BLAST of CmaCh06G010150 vs. TAIR 10
Match: AT1G01300.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 630.2 bits (1624), Expect = 1.4e-180
Identity = 328/476 (68.91%), Postives = 380/476 (79.83%), Query Frame = 0

Query: 13  FLLTLLSLSTAFSDFQTLIPK--PLP-ASPSLFSPESDTDSESFISSE----------AG 72
           F L+L S S +   FQTL P    LP ASP  F P  D+DSES + SE          + 
Sbjct: 15  FFLSLPSFS-SLPSFQTLFPNSHSLPCASPVSFQP--DSDSESLLESEFESGSDSESSSS 74

Query: 73  LELQLHHLDALSLNRTPEELFHLRLQRDALRVTKLSSLSGGSQNLSQASGTSHGTTGFSS 132
           + L L H+DALS N+TP+ELF  RLQRD+ RV  +++L+      +       G  GFSS
Sbjct: 75  ITLNLDHIDALSSNKTPDELFSSRLQRDSRRVKSIATLAAQIPGRNVTHAPRPG--GFSS 134

Query: 133 SVISGLAQGSGEYFTRIGVGTPPKYIYMVLDTGSDIVWLQCAPCKNCYSQTDPVFNPVKS 192
           SV+SGL+QGSGEYFTR+GVGTP +Y+YMVLDTGSDIVWLQCAPC+ CYSQ+DP+F+P KS
Sbjct: 135 SVVSGLSQGSGEYFTRLGVGTPARYVYMVLDTGSDIVWLQCAPCRRCYSQSDPIFDPRKS 194

Query: 193 RSYSKVLCRTPLCLRLESPGCN-QKQTCLYQVSYGDGSYTTGEFVTETLTFRRTKVERVA 252
           ++Y+ + C +P C RL+S GCN +++TCLYQVSYGDGS+T G+F TETLTFRR +V+ VA
Sbjct: 195 KTYATIPCSSPHCRRLDSAGCNTRRKTCLYQVSYGDGSFTVGDFSTETLTFRRNRVKGVA 254

Query: 253 LGCGHDNEGLFVGAAGLLGLGRGGLSFPSQAGRSFNQKFSYCLVDRSASSKPSSVVFGDS 312
           LGCGHDNEGLFVGAAGLLGLG+G LSFP Q G  FNQKFSYCLVDRSASSKPSSVVFG++
Sbjct: 255 LGCGHDNEGLFVGAAGLLGLGKGKLSFPGQTGHRFNQKFSYCLVDRSASSKPSSVVFGNA 314

Query: 313 AVSRTARFTPLLTNPRLDTFYYVELLGISVGGTPVSRISASHFKLDSNGNGGVIIDCGTS 372
           AVSR ARFTPLL+NP+LDTFYYV LLGISVGGT V  ++AS FKLD  GNGGVIID GTS
Sbjct: 315 AVSRIARFTPLLSNPKLDTFYYVGLLGISVGGTRVPGVTASLFKLDQIGNGGVIIDSGTS 374

Query: 373 VTRLNRPAYIALRNAFRAGASSLKLAPEFSLFDTCYDLSGKTTVKVPTVVLHFRGADVSL 432
           VTRL RPAYIA+R+AFR GA +LK AP+FSLFDTC+DLS    VKVPTVVLHFRGADVSL
Sbjct: 375 VTRLIRPAYIAMRDAFRVGAKTLKRAPDFSLFDTCFDLSNMNEVKVPTVVLHFRGADVSL 434

Query: 433 PASNYLIPVDDNGRFCFAFAGTTSGLSIIGNIQQQGFRVVYDLAGSRVGFSPRGCA 475
           PA+NYLIPVD NG+FCFAFAGT  GLSIIGNIQQQGFRVVYDLA SRVGF+P GCA
Sbjct: 435 PATNYLIPVDTNGKFCFAFAGTMGGLSIIGNIQQQGFRVVYDLASSRVGFAPGGCA 485

BLAST of CmaCh06G010150 vs. TAIR 10
Match: AT3G61820.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 573.9 bits (1478), Expect = 1.2e-163
Identity = 299/483 (61.90%), Postives = 360/483 (74.53%), Query Frame = 0

Query: 1   MEAKTSALPFIGFLLTLLSLSTAFSDFQTLIPKPLPASPSLFSPESDT-DSESFISSEAG 60
           ME K            L   S+A S +QTL+   LP+S +L  PES++   ES   S   
Sbjct: 1   MERKVLNTLAFSVFAVLFFTSSASSQYQTLVVNTLPSSATLSWPESESLTDESLSESTTS 60

Query: 61  LELQLHHLDALS--LNRTPEELFHLRLQRDALRVTKLSSLSGGSQNLSQASGTSHGTTGF 120
           L + L H+DALS   + +P +LF+LRLQRD+LRV  ++SL+  S   +    T     GF
Sbjct: 61  LSVHLSHVDALSSFSDASPADLFNLRLQRDSLRVKSITSLAAVSTGRNATKRTPRTAGGF 120

Query: 121 SSSVISGLAQGSGEYFTRIGVGTPPKYIYMVLDTGSDIVWLQCAPCKNCYSQTDPVFNPV 180
           S +VISGL+QGSGEYF R+GVGTP   +YMVLDTGSD+VWLQC+PCK CY+QTD +F+P 
Sbjct: 121 SGAVISGLSQGSGEYFMRLGVGTPATNVYMVLDTGSDVVWLQCSPCKACYNQTDAIFDPK 180

Query: 181 KSRSYSKVLCRTPLCLRL-ESPGC--NQKQTCLYQVSYGDGSYTTGEFVTETLTFRRTKV 240
           KS++++ V C + LC RL +S  C   + +TCLYQVSYGDGS+T G+F TETLTF   +V
Sbjct: 181 KSKTFATVPCGSRLCRRLDDSSECVTRRSKTCLYQVSYGDGSFTEGDFSTETLTFHGARV 240

Query: 241 ERVALGCGHDNEGLFVGAAGLLGLGRGGLSFPSQAGRSFNQKFSYCLVDR----SASSKP 300
           + V LGCGHDNEGLFVGAAGLLGLGRGGLSFPSQ    +N KFSYCLVDR    S+S  P
Sbjct: 241 DHVPLGCGHDNEGLFVGAAGLLGLGRGGLSFPSQTKNRYNGKFSYCLVDRTSSGSSSKPP 300

Query: 301 SSVVFGDSAVSRTARFTPLLTNPRLDTFYYVELLGISVGGTPVSRISASHFKLDSNGNGG 360
           S++VFG++AV +T+ FTPLLTNP+LDTFYY++LLGISVGG+ V  +S S FKLD+ GNGG
Sbjct: 301 STIVFGNAAVPKTSVFTPLLTNPKLDTFYYLQLLGISVGGSRVPGVSESQFKLDATGNGG 360

Query: 361 VIIDCGTSVTRLNRPAYIALRNAFRAGASSLKLAPEFSLFDTCYDLSGKTTVKVPTVVLH 420
           VIID GTSVTRL +PAY+ALR+AFR GA+ LK AP +SLFDTC+DLSG TTVKVPTVV H
Sbjct: 361 VIIDSGTSVTRLTQPAYVALRDAFRLGATKLKRAPSYSLFDTCFDLSGMTTVKVPTVVFH 420

Query: 421 FRGADVSLPASNYLIPVDDNGRFCFAFAGTTSGLSIIGNIQQQGFRVVYDLAGSRVGFSP 474
           F G +VSLPASNYLIPV+  GRFCFAFAGT   LSIIGNIQQQGFRV YDL GSRVGF  
Sbjct: 421 FGGGEVSLPASNYLIPVNTEGRFCFAFAGTMGSLSIIGNIQQQGFRVAYDLVGSRVGFLS 480

BLAST of CmaCh06G010150 vs. TAIR 10
Match: AT3G18490.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 398.7 bits (1023), Expect = 6.7e-111
Identity = 228/471 (48.41%), Postives = 300/471 (63.69%), Query Frame = 0

Query: 17  LLSLSTAFSDFQTLIP-KPLPASPSLFSPESDTDSESFISSEAGLELQLHHLDAL--SLN 76
           +L + ++    QT++   P  +S +   PES +D   F +S + L L+LH  D    S +
Sbjct: 37  VLDVVSSLQQTQTILSLDPTRSSLTTTKPESLSD-PVFFNSSSPLSLELHSRDTFVASQH 96

Query: 77  RTPEELFHLRLQRDALRVTKL-SSLSGGSQNLSQA-------SGTSHGTTGFSSSVISGL 136
           +  + L   RL+RD+ RV  + + +    + + ++         T + T   ++ V+SG 
Sbjct: 97  KDYKSLTLSRLERDSSRVAGIVAKIRFAVEGVDRSDLKPVYNEDTRYQTEDLTTPVVSGA 156

Query: 137 AQGSGEYFTRIGVGTPPKYIYMVLDTGSDIVWLQCAPCKNCYSQTDPVFNPVKSRSYSKV 196
           +QGSGEYF+RIGVGTP K +Y+VLDTGSD+ W+QC PC +CY Q+DPVFNP  S +Y  +
Sbjct: 157 SQGSGEYFSRIGVGTPAKEMYLVLDTGSDVNWIQCEPCADCYQQSDPVFNPTSSSTYKSL 216

Query: 197 LCRTPLCLRLESPGCNQKQTCLYQVSYGDGSYTTGEFVTETLTFRRT-KVERVALGCGHD 256
            C  P C  LE+  C   + CLYQVSYGDGS+T GE  T+T+TF  + K+  VALGCGHD
Sbjct: 217 TCSAPQCSLLETSACRSNK-CLYQVSYGDGSFTVGELATDTVTFGNSGKINNVALGCGHD 276

Query: 257 NEGLFVGAAGLLGLGRGGLSFPSQAGRSFNQKFSYCLVDRSASSKPSSVVFGDSAVSRTA 316
           NEGLF GAAGLLGLG G LS  +Q   +    FSYCLVDR  S K SS+ F    +    
Sbjct: 277 NEGLFTGAAGLLGLGGGVLSITNQMKAT---SFSYCLVDRD-SGKSSSLDFNSVQLGGGD 336

Query: 317 RFTPLLTNPRLDTFYYVELLGISVGGTPVSRISASHFKLDSNGNGGVIIDCGTSVTRLNR 376
              PLL N ++DTFYYV L G SVGG  V  +  + F +D++G+GGVI+DCGT+VTRL  
Sbjct: 337 ATAPLLRNKKIDTFYYVGLSGFSVGGEKVV-LPDAIFDVDASGSGGVILDCGTAVTRLQT 396

Query: 377 PAYIALRNAFRAGASSLKL-APEFSLFDTCYDLSGKTTVKVPTVVLHFRGA-DVSLPASN 436
            AY +LR+AF     +LK  +   SLFDTCYD S  +TVKVPTV  HF G   + LPA N
Sbjct: 397 QAYNSLRDAFLKLTVNLKKGSSSISLFDTCYDFSSLSTVKVPTVAFHFTGGKSLDLPAKN 456

Query: 437 YLIPVDDNGRFCFAFAGTTSGLSIIGNIQQQGFRVVYDLAGSRVGFSPRGC 474
           YLIPVDD+G FCFAFA T+S LSIIGN+QQQG R+ YDL+ + +G S   C
Sbjct: 457 YLIPVDDSGTFCFAFAPTSSSLSIIGNVQQQGTRITYDLSKNVIGLSGNKC 500

BLAST of CmaCh06G010150 vs. TAIR 10
Match: AT1G25510.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 397.1 bits (1019), Expect = 1.9e-110
Identity = 234/490 (47.76%), Postives = 298/490 (60.82%), Query Frame = 0

Query: 9   PFIGFLLTLLSLSTAFSDFQTLIPKPLPASPSLFSPESDTDSESFISS------------ 68
           P   F   +  L++  S F  ++P+    + S+ +         + SS            
Sbjct: 3   PNYSFFFFIFFLTSHSSVFSRILPETSTTTTSILNVADSIHRTKYTSSFRLNQQEEQTHS 62

Query: 69  -EAGLELQLHHLDALSLNRTP----EELFHLRLQRDALRVTKL-SSLSGGSQNLSQAS-- 128
             +   LQLH    +S+  T     + L   RL RD  RV  L + L     N+S+A   
Sbjct: 63  ASSSFSLQLH--SRVSVRGTEHSDYKSLTLARLNRDTARVKSLITRLDLAINNISKADLK 122

Query: 129 --GTSHGT--TGFSSSVISGLAQGSGEYFTRIGVGTPPKYIYMVLDTGSDIVWLQCAPCK 188
              T + T      + +ISG  QGSGEYFTR+G+G P + +YMVLDTGSD+ WLQC PC 
Sbjct: 123 PISTMYTTEEQDIEAPLISGTTQGSGEYFTRVGIGKPAREVYMVLDTGSDVNWLQCTPCA 182

Query: 189 NCYSQTDPVFNPVKSRSYSKVLCRTPLCLRLESPGCNQKQTCLYQVSYGDGSYTTGEFVT 248
           +CY QT+P+F P  S SY  + C TP C  LE   C +  TCLY+VSYGDGSYT G+F T
Sbjct: 183 DCYHQTEPIFEPSSSSSYEPLSCDTPQCNALEVSEC-RNATCLYEVSYGDGSYTVGDFAT 242

Query: 249 ETLTFRRTKVERVALGCGHDNEGLFVGAAGLLGLGRGGLSFPSQAGRSFNQKFSYCLVDR 308
           ETLT   T V+ VA+GCGH NEGLFVGAAGLLGLG G L+ PSQ   +    FSYCLVDR
Sbjct: 243 ETLTIGSTLVQNVAVGCGHSNEGLFVGAAGLLGLGGGLLALPSQLNTT---SFSYCLVDR 302

Query: 309 SASSKPSSVVFGDSAVSRTARFTPLLTNPRLDTFYYVELLGISVGGTPVSRISASHFKLD 368
            + S  S+V FG S +S  A   PLL N +LDTFYY+ L GISVGG  + +I  S F++D
Sbjct: 303 DSDS-ASTVDFGTS-LSPDAVVAPLLRNHQLDTFYYLGLTGISVGG-ELLQIPQSSFEMD 362

Query: 369 SNGNGGVIIDCGTSVTRLNRPAYIALRNAFRAGASSLKLAPEFSLFDTCYDLSGKTTVKV 428
            +G+GG+IID GT+VTRL    Y +LR++F  G   L+ A   ++FDTCY+LS KTTV+V
Sbjct: 363 ESGSGGIIIDSGTAVTRLQTEIYNSLRDSFVKGTLDLEKAAGVAMFDTCYNLSAKTTVEV 422

Query: 429 PTVVLHFRGAD-VSLPASNYLIPVDDNGRFCFAFAGTTSGLSIIGNIQQQGFRVVYDLAG 474
           PTV  HF G   ++LPA NY+IPVD  G FC AFA T S L+IIGN+QQQG RV +DLA 
Sbjct: 423 PTVAFHFPGGKMLALPAKNYMIPVDSVGTFCLAFAPTASSLAIIGNVQQQGTRVTFDLAN 482

BLAST of CmaCh06G010150 vs. TAIR 10
Match: AT3G20015.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 385.6 bits (989), Expect = 5.9e-107
Identity = 218/474 (45.99%), Postives = 291/474 (61.39%), Query Frame = 0

Query: 8   LPFIGFLLTL-----LSLSTAFSDFQTLIPKPLPASPSLFSPESDTDSESFISSEAGLEL 67
           LP   F L L      S S +F DFQ +     P + +   P+ +    S  SS +   L
Sbjct: 3   LPLFFFFLHLHLHLSSSSSISFPDFQIIDVLQPPLTVTATLPDFNNTHFSDESS-SKYTL 62

Query: 68  QLHHLDALS--LNRTPEELFHLRLQRDALRVTKLSSLSGGSQNLSQASGTSHGTTGFSSS 127
           +L H D       R      H R++RD  RV+ +  L   S  +  +S + +    F S 
Sbjct: 63  RLLHRDRFPSVTYRNHHHRLHARMRRDTDRVSAI--LRRISGKVIPSSDSRYEVNDFGSD 122

Query: 128 VISGLAQGSGEYFTRIGVGTPPKYIYMVLDTGSDIVWLQCAPCKNCYSQTDPVFNPVKSR 187
           ++SG+ QGSGEYF RIGVG+PP+  YMV+D+GSD+VW+QC PCK CY Q+DPVF+P KS 
Sbjct: 123 IVSGMDQGSGEYFVRIGVGSPPRDQYMVIDSGSDMVWVQCQPCKLCYKQSDPVFDPAKSG 182

Query: 188 SYSKVLCRTPLCLRLESPGCNQKQTCLYQVSYGDGSYTTGEFVTETLTFRRTKVERVALG 247
           SY+ V C + +C R+E+ GC+    C Y+V YGDGSYT G    ETLTF +T V  VA+G
Sbjct: 183 SYTGVSCGSSVCDRIENSGCH-SGGCRYEVMYGDGSYTKGTLALETLTFAKTVVRNVAMG 242

Query: 248 CGHDNEGLFVGAAGLLGLGRGGLSFPSQAGRSFNQKFSYCLVDRSASSKPSSVVFGDSAV 307
           CGH N G+F+GAAGLLG+G G +SF  Q        F YCLV R   S   S+VFG  A+
Sbjct: 243 CGHRNRGMFIGAAGLLGIGGGSMSFVGQLSGQTGGAFGYCLVSRGTDS-TGSLVFGREAL 302

Query: 308 SRTARFTPLLTNPRLDTFYYVELLGISVGGTPVSRISASHFKLDSNGNGGVIIDCGTSVT 367
              A + PL+ NPR  +FYYV L G+ VGG  +  +    F L   G+GGV++D GT+VT
Sbjct: 303 PVGASWVPLVRNPRAPSFYYVGLKGLGVGGVRIP-LPDGVFDLTETGDGGVVMDTGTAVT 362

Query: 368 RLNRPAYIALRNAFRAGASSLKLAPEFSLFDTCYDLSGKTTVKVPTVVLHF-RGADVSLP 427
           RL   AY+A R+ F++  ++L  A   S+FDTCYDLSG  +V+VPTV  +F  G  ++LP
Sbjct: 363 RLPTAAYVAFRDGFKSQTANLPRASGVSIFDTCYDLSGFVSVRVPTVSFYFTEGPVLTLP 422

Query: 428 ASNYLIPVDDNGRFCFAFAGTTSGLSIIGNIQQQGFRVVYDLAGSRVGFSPRGC 474
           A N+L+PVDD+G +CFAFA + +GLSIIGNIQQ+G +V +D A   VGF P  C
Sbjct: 423 ARNFLMPVDDSGTYCFAFAASPTGLSIIGNIQQEGIQVSFDGANGFVGFGPNVC 470

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LNJ31.9e-17968.91Aspartyl protease family protein 2 OS=Arabidopsis thaliana OX=3702 GN=APF2 PE=2 ... [more]
Q9LS409.4e-11048.41Protein ASPARTIC PROTEASE IN GUARD CELL 1 OS=Arabidopsis thaliana OX=3702 GN=ASP... [more]
Q9LHE38.3e-10645.99Protein ASPARTIC PROTEASE IN GUARD CELL 2 OS=Arabidopsis thaliana OX=3702 GN=ASP... [more]
Q8S9J62.1e-7238.50Aspartyl protease family protein At5g10770 OS=Arabidopsis thaliana OX=3702 GN=At... [more]
Q766C32.7e-7239.05Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis OX=150966 GN=nep1 PE=1 S... [more]
Match NameE-valueIdentityDescription
A0A6J1IFR81.4e-270100.00aspartyl protease family protein 2-like OS=Cucurbita maxima OX=3661 GN=LOC111474... [more]
A0A6J1IJ021.6e-26699.16aspartyl protease family protein 2-like OS=Cucurbita maxima OX=3661 GN=LOC111473... [more]
A0A6J1G0Y11.4e-26598.52aspartyl protease family protein 2-like OS=Cucurbita moschata OX=3662 GN=LOC1114... [more]
A0A6J1EFG49.8e-23587.68aspartyl protease family protein 2-like OS=Cucurbita moschata OX=3662 GN=LOC1114... [more]
A0A6J1IY261.3e-23487.45aspartyl protease family protein 2-like OS=Cucurbita maxima OX=3661 GN=LOC111479... [more]
Match NameE-valueIdentityDescription
XP_022975090.13.0e-270100.00aspartyl protease family protein 2-like [Cucurbita maxima][more]
XP_022974939.13.4e-26699.16aspartyl protease family protein 2-like [Cucurbita maxima][more]
XP_023538763.11.7e-26598.10aspartyl protease family protein 2-like [Cucurbita pepo subsp. pepo][more]
XP_022945440.12.9e-26598.52aspartyl protease family protein 2-like [Cucurbita moschata][more]
KAG6597078.15.1e-26297.68Aspartyl protease family protein 2, partial [Cucurbita argyrosperma subsp. soror... [more]
Match NameE-valueIdentityDescription
AT1G01300.11.4e-18068.91Eukaryotic aspartyl protease family protein [more]
AT3G61820.11.2e-16361.90Eukaryotic aspartyl protease family protein [more]
AT3G18490.16.7e-11148.41Eukaryotic aspartyl protease family protein [more]
AT1G25510.11.9e-11047.76Eukaryotic aspartyl protease family protein [more]
AT3G20015.15.9e-10745.99Eukaryotic aspartyl protease family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001461Aspartic peptidase A1 familyPRINTSPR00792PEPSINcoord: 445..460
score: 26.83
coord: 351..362
score: 39.97
coord: 138..158
score: 40.67
IPR001461Aspartic peptidase A1 familyPANTHERPTHR13683ASPARTYL PROTEASEScoord: 50..473
IPR032861Xylanase inhibitor, N-terminalPFAMPF14543TAXi_Ncoord: 132..297
e-value: 2.4E-55
score: 187.5
IPR021109Aspartic peptidase domain superfamilyGENE3D2.40.70.10Acid Proteasescoord: 295..474
e-value: 6.4E-54
score: 184.5
IPR021109Aspartic peptidase domain superfamilyGENE3D2.40.70.10Acid Proteasescoord: 103..294
e-value: 2.5E-55
score: 189.5
IPR021109Aspartic peptidase domain superfamilySUPERFAMILY50630Acid proteasescoord: 125..473
IPR032799Xylanase inhibitor, C-terminalPFAMPF14541TAXi_Ccoord: 318..469
e-value: 8.4E-36
score: 123.2
NoneNo IPR availablePANTHERPTHR13683:SF761ASPARTYL PROTEASE FAMILY PROTEIN 2-LIKEcoord: 50..473
IPR001969Aspartic peptidase, active sitePROSITEPS00141ASP_PROTEASEcoord: 147..158
IPR033121Peptidase family A1 domainPROSITEPS51767PEPTIDASE_A1coord: 132..469
score: 46.568588
IPR033873CND41-likeCDDcd05472cnd41_likecoord: 131..473
e-value: 5.88536E-137
score: 394.331

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh06G010150.1CmaCh06G010150.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
molecular_function GO:0004190 aspartic-type endopeptidase activity