CmaCh06G009150 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh06G009150
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
DescriptionL-ascorbate oxidase homolog
LocationCma_Chr06: 6040179 .. 6041837 (+)
RNA-Seq ExpressionCmaCh06G009150
SyntenyCmaCh06G009150
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTGGGTTGACGTTCACCATGCTCCGAATTTGCCTGTTGGCTGTAGCACTGTCCACAGTCCGAGGCGAGGATCCTTATTTCTTCTTCACATGGAACGTCACCTACGGCACCATCTCTCCTTTGGGTGTTCCCCAACAAGGCATCCTCATCAATGGTCAATTCCCCGGACCTAATATCAACTCCACATCCAACAATAACATAGTCGTCAATGTCTTCAACAATCTCGATGAGCCCTTCCTTTTACACTGGAGCGGTGTTCAACATAGAAAGAACTCATGGCAAGATGGTCTACTCGGAACAAATTGCCCAATCCCACCAGGCTCTAACTTCACCTACCATTTCCAAGTCAAGGACCAAATTGGCAGCTTCTTCTACTATCCATCCACTGCCATGCATAGAGCTGGTGGAGCCTTCGGTGGCCTTCGTATCAATAGCCGTTTACTCATCCCCGTCCCTTACGCAGACCCAGAAGATGATTACACTGTCCTAGCTGGTGACTGGTACACCAAGACTCACTCCACTCTTAAGAAGCTCTTGGATAGCGGCCGCTCAATTGCTAGACCCGACGGTGTCCTCATTAATGGCAAGACCGCAAAGGGTGATGGAACCGATGAGCCACTCTTCACCATGAAGCCCGGGAAGACTTACAAGTATAGAATTTGCAACGTTGGACTCAAGACATCTCTTAATTTCAGGATCCAAGGCCACACCATGAAGTTGGTTGAGATGGAGGGCTCACACACTGTCCAAAACGTTTACCAATCACTCGATGTTCATGTGGGACAATGCTTTTCAGTTCTAGTCACAGCCGACCAAGAACCCAAGGACTATTACATGGTGGCATCCACTCGATTCATTAAGAACATTCTTGTCGGTAAAGCCATTGTTCGATACACTAATGGAAATAGTCCTGCCTCCCCTGAAATTCCAGAGGCACCAATTGGTTGGGCGTGGTCACTCAATCAATTCCGTACATTTCGTTGGAACCTTACAGCCAGTGCAGCTAGACCCAATCCTCAAGGCTCATACCACTATGGTTCCATCAACATCACCCGCACCATCAAGCTGCTCAATTCTGCCACTCTGGTTGATGGTAAGTTGAGATATGCTATCAATGATGTCTCTCATGTCGACCCTGAAACTCCATTGAAGCTTGCACAGTATTTCAATGTCGCTGACAATGTTTTCAAGTATGATATGATTTCTGACGAGGGATCGGTAGAAGGAAGAGATGTGACTATTGCTCCTAATGTTGTTAATGCAACATACCGTAACTTCGTTGAGATTATATTTGAGAATCATGAGAAGAGTATGCAATCGTGGCATTTGGATGGGTACTCGTTCTTTGCTGTTGCAATTGAGCCTGGGAGATGGGCTCCGGAGAAACGAAAGAACTACAATCTTCTTGATGCGGTGCATAGGCATACAATTCAAGTGTTCCCTAAATCATGGGCAGCTGTTCTTGTTACATTGGACAATGCAGGAATGTGGAACTTGAGGTCTGAACTGACAGAGAATCGTTACTTGGGACAACAACTGTACTTCAGTGTTCTTTCACCGGCTAACTCCCTTAGAGATGAATACAACATTCCAGACAACACATTACTCTGCGGTTTGGTGAAGGACATGCCATTGCCGAAGCCATACACCATTTGA

mRNA sequence

ATGGCTGGGTTGACGTTCACCATGCTCCGAATTTGCCTGTTGGCTGTAGCACTGTCCACAGTCCGAGGCGAGGATCCTTATTTCTTCTTCACATGGAACGTCACCTACGGCACCATCTCTCCTTTGGGTGTTCCCCAACAAGGCATCCTCATCAATGGTCAATTCCCCGGACCTAATATCAACTCCACATCCAACAATAACATAGTCGTCAATGTCTTCAACAATCTCGATGAGCCCTTCCTTTTACACTGGAGCGGTGTTCAACATAGAAAGAACTCATGGCAAGATGGTCTACTCGGAACAAATTGCCCAATCCCACCAGGCTCTAACTTCACCTACCATTTCCAAGTCAAGGACCAAATTGGCAGCTTCTTCTACTATCCATCCACTGCCATGCATAGAGCTGGTGGAGCCTTCGGTGGCCTTCGTATCAATAGCCGTTTACTCATCCCCGTCCCTTACGCAGACCCAGAAGATGATTACACTGTCCTAGCTGGTGACTGGTACACCAAGACTCACTCCACTCTTAAGAAGCTCTTGGATAGCGGCCGCTCAATTGCTAGACCCGACGGTGTCCTCATTAATGGCAAGACCGCAAAGGGTGATGGAACCGATGAGCCACTCTTCACCATGAAGCCCGGGAAGACTTACAAGTATAGAATTTGCAACGTTGGACTCAAGACATCTCTTAATTTCAGGATCCAAGGCCACACCATGAAGTTGGTTGAGATGGAGGGCTCACACACTGTCCAAAACGTTTACCAATCACTCGATGTTCATGTGGGACAATGCTTTTCAGTTCTAGTCACAGCCGACCAAGAACCCAAGGACTATTACATGGTGGCATCCACTCGATTCATTAAGAACATTCTTGTCGGTAAAGCCATTGTTCGATACACTAATGGAAATAGTCCTGCCTCCCCTGAAATTCCAGAGGCACCAATTGGTTGGGCGTGGTCACTCAATCAATTCCGTACATTTCGTTGGAACCTTACAGCCAGTGCAGCTAGACCCAATCCTCAAGGCTCATACCACTATGGTTCCATCAACATCACCCGCACCATCAAGCTGCTCAATTCTGCCACTCTGGTTGATGGTAAGTTGAGATATGCTATCAATGATGTCTCTCATGTCGACCCTGAAACTCCATTGAAGCTTGCACAGTATTTCAATGTCGCTGACAATGTTTTCAAGTATGATATGATTTCTGACGAGGGATCGGTAGAAGGAAGAGATGTGACTATTGCTCCTAATGTTGTTAATGCAACATACCGTAACTTCGTTGAGATTATATTTGAGAATCATGAGAAGAGTATGCAATCGTGGCATTTGGATGGGTACTCGTTCTTTGCTGTTGCAATTGAGCCTGGGAGATGGGCTCCGGAGAAACGAAAGAACTACAATCTTCTTGATGCGGTGCATAGGCATACAATTCAAGTGTTCCCTAAATCATGGGCAGCTGTTCTTGTTACATTGGACAATGCAGGAATGTGGAACTTGAGGTCTGAACTGACAGAGAATCGTTACTTGGGACAACAACTGTACTTCAGTGTTCTTTCACCGGCTAACTCCCTTAGAGATGAATACAACATTCCAGACAACACATTACTCTGCGGTTTGGTGAAGGACATGCCATTGCCGAAGCCATACACCATTTGA

Coding sequence (CDS)

ATGGCTGGGTTGACGTTCACCATGCTCCGAATTTGCCTGTTGGCTGTAGCACTGTCCACAGTCCGAGGCGAGGATCCTTATTTCTTCTTCACATGGAACGTCACCTACGGCACCATCTCTCCTTTGGGTGTTCCCCAACAAGGCATCCTCATCAATGGTCAATTCCCCGGACCTAATATCAACTCCACATCCAACAATAACATAGTCGTCAATGTCTTCAACAATCTCGATGAGCCCTTCCTTTTACACTGGAGCGGTGTTCAACATAGAAAGAACTCATGGCAAGATGGTCTACTCGGAACAAATTGCCCAATCCCACCAGGCTCTAACTTCACCTACCATTTCCAAGTCAAGGACCAAATTGGCAGCTTCTTCTACTATCCATCCACTGCCATGCATAGAGCTGGTGGAGCCTTCGGTGGCCTTCGTATCAATAGCCGTTTACTCATCCCCGTCCCTTACGCAGACCCAGAAGATGATTACACTGTCCTAGCTGGTGACTGGTACACCAAGACTCACTCCACTCTTAAGAAGCTCTTGGATAGCGGCCGCTCAATTGCTAGACCCGACGGTGTCCTCATTAATGGCAAGACCGCAAAGGGTGATGGAACCGATGAGCCACTCTTCACCATGAAGCCCGGGAAGACTTACAAGTATAGAATTTGCAACGTTGGACTCAAGACATCTCTTAATTTCAGGATCCAAGGCCACACCATGAAGTTGGTTGAGATGGAGGGCTCACACACTGTCCAAAACGTTTACCAATCACTCGATGTTCATGTGGGACAATGCTTTTCAGTTCTAGTCACAGCCGACCAAGAACCCAAGGACTATTACATGGTGGCATCCACTCGATTCATTAAGAACATTCTTGTCGGTAAAGCCATTGTTCGATACACTAATGGAAATAGTCCTGCCTCCCCTGAAATTCCAGAGGCACCAATTGGTTGGGCGTGGTCACTCAATCAATTCCGTACATTTCGTTGGAACCTTACAGCCAGTGCAGCTAGACCCAATCCTCAAGGCTCATACCACTATGGTTCCATCAACATCACCCGCACCATCAAGCTGCTCAATTCTGCCACTCTGGTTGATGGTAAGTTGAGATATGCTATCAATGATGTCTCTCATGTCGACCCTGAAACTCCATTGAAGCTTGCACAGTATTTCAATGTCGCTGACAATGTTTTCAAGTATGATATGATTTCTGACGAGGGATCGGTAGAAGGAAGAGATGTGACTATTGCTCCTAATGTTGTTAATGCAACATACCGTAACTTCGTTGAGATTATATTTGAGAATCATGAGAAGAGTATGCAATCGTGGCATTTGGATGGGTACTCGTTCTTTGCTGTTGCAATTGAGCCTGGGAGATGGGCTCCGGAGAAACGAAAGAACTACAATCTTCTTGATGCGGTGCATAGGCATACAATTCAAGTGTTCCCTAAATCATGGGCAGCTGTTCTTGTTACATTGGACAATGCAGGAATGTGGAACTTGAGGTCTGAACTGACAGAGAATCGTTACTTGGGACAACAACTGTACTTCAGTGTTCTTTCACCGGCTAACTCCCTTAGAGATGAATACAACATTCCAGACAACACATTACTCTGCGGTTTGGTGAAGGACATGCCATTGCCGAAGCCATACACCATTTGA

Protein sequence

MAGLTFTMLRICLLAVALSTVRGEDPYFFFTWNVTYGTISPLGVPQQGILINGQFPGPNINSTSNNNIVVNVFNNLDEPFLLHWSGVQHRKNSWQDGLLGTNCPIPPGSNFTYHFQVKDQIGSFFYYPSTAMHRAGGAFGGLRINSRLLIPVPYADPEDDYTVLAGDWYTKTHSTLKKLLDSGRSIARPDGVLINGKTAKGDGTDEPLFTMKPGKTYKYRICNVGLKTSLNFRIQGHTMKLVEMEGSHTVQNVYQSLDVHVGQCFSVLVTADQEPKDYYMVASTRFIKNILVGKAIVRYTNGNSPASPEIPEAPIGWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKLLNSATLVDGKLRYAINDVSHVDPETPLKLAQYFNVADNVFKYDMISDEGSVEGRDVTIAPNVVNATYRNFVEIIFENHEKSMQSWHLDGYSFFAVAIEPGRWAPEKRKNYNLLDAVHRHTIQVFPKSWAAVLVTLDNAGMWNLRSELTENRYLGQQLYFSVLSPANSLRDEYNIPDNTLLCGLVKDMPLPKPYTI
Homology
BLAST of CmaCh06G009150 vs. ExPASy Swiss-Prot
Match: Q00624 (L-ascorbate oxidase homolog OS=Brassica napus OX=3708 GN=Bp10 PE=2 SV=1)

HSP 1 Score: 872.8 bits (2254), Expect = 2.0e-252
Identity = 394/552 (71.38%), Postives = 461/552 (83.51%), Query Frame = 0

Query: 1   MAGLTFTMLRICLLAVALSTVRGEDPYFFFTWNVTYGTISPLGVPQQGILINGQFPGPNI 60
           M G+      + L A A   V  EDPYF   WNVTYGT SPLGVPQQ ILINGQFPGPNI
Sbjct: 1   MRGVKLLAACLYLAAAATVVVHAEDPYFHHVWNVTYGTASPLGVPQQVILINGQFPGPNI 60

Query: 61  NSTSNNNIVVNVFNNLDEPFLLHWSGVQHRKNSWQDGLLGTNCPIPPGSNFTYHFQVKDQ 120
           NSTSNNN+++NVFNNLDEPFLL W+G+QHRKN WQDG  GT CPI PG+N+TYHFQ KDQ
Sbjct: 61  NSTSNNNVIINVFNNLDEPFLLTWNGIQHRKNCWQDGTPGTMCPIMPGTNYTYHFQPKDQ 120

Query: 121 IGSFFYYPSTAMHRAGGAFGGLRINSRLLIPVPYADPEDDYTVLAGDWYTKTHSTLKKLL 180
           IGS+FYYP+T MHRA G +GGLR+NSRLLIPVPYADPEDDYTVL GDWYTK+H+ LKK L
Sbjct: 121 IGSYFYYPTTGMHRAAGGYGGLRVNSRLLIPVPYADPEDDYTVLIGDWYTKSHTQLKKFL 180

Query: 181 DSGRSIARPDGVLINGKTAKGDGTDEPLFTMKPGKTYKYRICNVGLKTSLNFRIQGHTMK 240
           D GR+I RPDG++INGK+ KGDG+D PLFT+KPGKTY+ RICNVG+KTS+NFRIQ H MK
Sbjct: 181 DGGRTIGRPDGIVINGKSGKGDGSDAPLFTLKPGKTYRVRICNVGVKTSINFRIQNHKMK 240

Query: 241 LVEMEGSHTVQNVYQSLDVHVGQCFSVLVTADQEPKDYYMVASTRFIKNILVGKAIVRYT 300
           LVEMEGSH +QN Y SLDVHVGQCF  +VTA+QEPKDYYMVAS+RF+K ++    ++RY 
Sbjct: 241 LVEMEGSHVLQNDYDSLDVHVGQCFGTIVTANQEPKDYYMVASSRFLKTVITTTGLLRYE 300

Query: 301 NGNSPASPEIPEAPIGWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKLLNS 360
            G  PAS ++P  P+GWAWSLNQFR+FRWNLTASAARPNPQGSYHYG INITRTIKL+N+
Sbjct: 301 GGKGPASSQLPAGPVGWAWSLNQFRSFRWNLTASAARPNPQGSYHYGKINITRTIKLVNT 360

Query: 361 ATLVDGKLRYAINDVSHVDPETPLKLAQYFNVADNVFKYDMISDEGSVEG-RDVTIAPNV 420
              VDGKLR+A+N VSH +PETPLKLA+YF ++D VFKYD I+D+ + E  +++ I PNV
Sbjct: 361 QGKVDGKLRFALNGVSHTEPETPLKLAEYFGISDKVFKYDTITDDPTPEQIKNIKIEPNV 420

Query: 421 VNATYRNFVEIIFENHEKSMQSWHLDGYSFFAVAIEPGRWAPEKRKNYNLLDAVHRHTIQ 480
           +N T+R FVE++FENHEKS+QSWHLDGYSFF+VA+EPG W PEKRKNYNLLDAV RHT+Q
Sbjct: 421 LNITHRTFVEVVFENHEKSVQSWHLDGYSFFSVAVEPGTWTPEKRKNYNLLDAVSRHTVQ 480

Query: 481 VFPKSWAAVLVTLDNAGMWNLRSELTENRYLGQQLYFSVLSPANSLRDEYNIPDNTLLCG 540
           V+PK WAA+L+T DN GMWN+RSE TE RYLGQQLY SVLSP  SLRDEYN+P+ +L CG
Sbjct: 481 VYPKCWAAILLTFDNCGMWNVRSENTERRYLGQQLYASVLSPEKSLRDEYNMPETSLQCG 540

Query: 541 LVKDMPLP-KPY 551
           LVK+ P P  PY
Sbjct: 541 LVKNTPKPVNPY 552

BLAST of CmaCh06G009150 vs. ExPASy Swiss-Prot
Match: P29162 (L-ascorbate oxidase homolog OS=Nicotiana tabacum OX=4097 PE=2 SV=1)

HSP 1 Score: 825.5 bits (2131), Expect = 3.6e-238
Identity = 375/551 (68.06%), Postives = 446/551 (80.94%), Query Frame = 0

Query: 4   LTFTMLRICLLAVALSTVRGEDPYFFFTWNVTYGTISPLGVPQQGILINGQFPGPNINST 63
           +TF  L +CL    ++    EDPY +F WNVTYGTI+PLGVPQQGILINGQFPGP IN T
Sbjct: 6   VTFVALLLCLSVGVIA----EDPYLYFNWNVTYGTIAPLGVPQQGILINGQFPGPRINCT 65

Query: 64  SNNNIVVNVFNNLDEPFLLHWSGVQHRKNSWQDGLLGTNCPIPPGSNFTYHFQVKDQIGS 123
           SNNNIVVNVFNNLDEPFL  W+GVQHRKNSWQDG  GT CPI PG NFTY FQVKDQIGS
Sbjct: 66  SNNNIVVNVFNNLDEPFLFTWNGVQHRKNSWQDGTPGTMCPIMPGQNFTYRFQVKDQIGS 125

Query: 124 FFYYPSTAMHRAGGAFGGLRINSRLLIPVPYADPEDDYTVLAGDWYTKTHSTLKKLLDSG 183
           + Y+P+TA+HRA G +G L ++SR LIPVP+ +P D+Y V  GDWY K H TLKK+LD G
Sbjct: 126 YSYFPTTALHRAAGGYGALNVHSRALIPVPFDNPADEYNVFVGDWYNKGHKTLKKILDGG 185

Query: 184 RSIARPDGVLINGKTAKGDGTDEPLFTMKPGKTYKYRICNVGLKTSLNFRIQGHTMKLVE 243
           R+I RPDG++INGK+AK     EPLFTM+ GKTY+YR CN+G+++S+N R QGH MKLVE
Sbjct: 186 RTIGRPDGIIINGKSAKVGEAKEPLFTMEAGKTYRYRFCNLGMRSSVNIRFQGHPMKLVE 245

Query: 244 MEGSHTVQNVYQSLDVHVGQCFSVLVTADQEPKDYYMVASTRFIKNILVGKAIVRYTNGN 303
           +EGSHTVQN+Y SLD+HVGQC SVLVTADQEPKDYY+V S+RF+K  L   AI+RY NG 
Sbjct: 246 LEGSHTVQNIYDSLDLHVGQCLSVLVTADQEPKDYYLVVSSRFLKQALSSVAIIRYANGK 305

Query: 304 SPASPEIPEAP----IGWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKLLN 363
            PASPE+P  P     G AWS+NQFR+FRWNLTASAARPNPQGSYHYG INITRTIK+ N
Sbjct: 306 GPASPELPTPPPENTEGIAWSMNQFRSFRWNLTASAARPNPQGSYHYGQINITRTIKIFN 365

Query: 364 SATLVDGKLRYAINDVSHVDPETPLKLAQYFNVADNVFKYDMISDEGSVEGRDVTIAPNV 423
           S + V GKLRY +N +SH + ETPLKL +YF   +  FKYD+++DE   +   +TIA NV
Sbjct: 366 SMSQVGGKLRYGLNGISHTNGETPLKLVEYFGATNKAFKYDLMADEAPADPSKLTIATNV 425

Query: 424 VNATYRNFVEIIFENHEKSMQSWHLDGYSFFAVAIEPGRWAPEKRKNYNLLDAVHRHTIQ 483
            NATYRNFVEIIFENHEK+++++HLDGYSFFAVA+EPGRW+PEKRKNYNL+D + R+ IQ
Sbjct: 426 KNATYRNFVEIIFENHEKTIRTYHLDGYSFFAVAVEPGRWSPEKRKNYNLVDGLSRNNIQ 485

Query: 484 VFPKSWAAVLVTLDNAGMWNLRSELTENRYLGQQLYFSVLSPANSLRDEYNIPDNTLLCG 543
           V+P SWAA+++T DNAGMWNLRSE+ E  YLG+QLYFSVLSP+ SLRDEYNIPDN  LCG
Sbjct: 486 VYPNSWAAIMLTFDNAGMWNLRSEMWEKTYLGEQLYFSVLSPSRSLRDEYNIPDNHPLCG 545

Query: 544 LVKDMPLPKPY 551
           +VK + +P PY
Sbjct: 546 IVKGLSMPAPY 552

BLAST of CmaCh06G009150 vs. ExPASy Swiss-Prot
Match: Q9SU40 (Monocopper oxidase-like protein SKU5 OS=Arabidopsis thaliana OX=3702 GN=SKU5 PE=1 SV=1)

HSP 1 Score: 554.7 bits (1428), Expect = 1.2e-156
Identity = 271/559 (48.48%), Postives = 362/559 (64.76%), Query Frame = 0

Query: 8   MLRICLLA--VALSTVRGEDPYFFFTWNVTYGTISPLGVPQQGILINGQFPGPNINSTSN 67
           + +I LL   V +S     DPY F+ + V+Y T SPLGVPQQ I ING+FPGP IN T+N
Sbjct: 3   LFKILLLVFFVNISFCFAADPYSFYNFEVSYITASPLGVPQQVIAINGKFPGPTINVTTN 62

Query: 68  NNIVVNVFNNLDEPFLLHWSGVQHRKNSWQDGLLGTNCPIPPGSNFTYHFQVKDQIGSFF 127
            N+VVNV N LDE  LLHW+G+Q R+ SWQDG+LGTNCPIPP  N+TY FQVKDQIGSFF
Sbjct: 63  ENLVVNVRNKLDEGLLLHWNGIQQRRVSWQDGVLGTNCPIPPKWNWTYEFQVKDQIGSFF 122

Query: 128 YYPSTAMHRAGGAFGGLRINSRLLIPVPYADPEDDYTVLAGDWYTKTHSTLKKLLDSGRS 187
           Y+PS    RA G FG   +N R +IPVP++ P+ D TV  GDWY + H+ L+K LD G+ 
Sbjct: 123 YFPSLHFQRASGGFGSFVVNPRAIIPVPFSTPDGDITVTIGDWYIRNHTALRKALDDGKD 182

Query: 188 IARPDGVLINGK-------TAKGDGTDEPLFTMKPGKTYKYRICNVGLKTSLNFRIQGHT 247
           +  PDGVLINGK       T   DG D    T+ PGKTY+ R+ NVG+ TSLNFRIQGH 
Sbjct: 183 LGMPDGVLINGKGPYRYNDTLVADGIDFETITVHPGKTYRLRVSNVGISTSLNFRIQGHN 242

Query: 248 MKLVEMEGSHTVQNVYQSLDVHVGQCFSVLVTADQE-PKDYYMVASTRFIKNIL----VG 307
           + L E EGS+TVQ  Y SLD+HVGQ +S LVT DQ    DYY+VAS R +   +     G
Sbjct: 243 LVLAESEGSYTVQQNYTSLDIHVGQSYSFLVTMDQNASSDYYIVASARVVNETIWRRVTG 302

Query: 308 KAIVRYTNGNSPASPEIPEAP---IGWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSIN 367
             I++YTN    A  ++P  P       +S+NQ R+ RWN++AS ARPNPQGS+ YGSIN
Sbjct: 303 VGILKYTNSKGKAKGQLPPGPQDEFDKTFSMNQARSIRWNVSASGARPNPQGSFKYGSIN 362

Query: 368 ITRTIKLLNSATL-VDGKLRYAINDVSHVDPETPLKLAQYFNVADNVFKYDMISDEGSVE 427
           +T    L N   + + GK R  +N +S  +P TP++LA    V D V+K D    +  + 
Sbjct: 363 VTDVYVLRNMPPVTISGKRRTTLNGISFKNPSTPIRLADKLKVKD-VYKLDF--PKRPLT 422

Query: 428 GRDVTIAPNVVNATYRNFVEIIFENHEKSMQSWHLDGYSFFAVAIEPGRWAPEKRKNYNL 487
           G    +A +++N TYR F+E++ +N++  MQS+H+ GY+FF V ++ G W    R  YN 
Sbjct: 423 G-PAKVATSIINGTYRGFMEVVLQNNDTKMQSYHMSGYAFFVVGMDYGEWTENSRGTYNK 482

Query: 488 LDAVHRHTIQVFPKSWAAVLVTLDNAGMWNLRSELTENRYLGQQLYFSVLSPANSLRDEY 547
            D + R TIQV+P +W+A+L++LDN G WNLR+E  ++ YLGQ+ Y  V++P  + + E+
Sbjct: 483 WDGIARSTIQVYPGAWSAILISLDNPGAWNLRTENLDSWYLGQETYVRVVNPDENNKTEF 542

Query: 548 NIPDNTLLCGLVKDMPLPK 549
             PDN L CG +  +  P+
Sbjct: 543 GHPDNVLYCGALSKLQKPQ 557

BLAST of CmaCh06G009150 vs. ExPASy Swiss-Prot
Match: Q8VXX5 (Monocopper oxidase-like protein SKS1 OS=Arabidopsis thaliana OX=3702 GN=SKS1 PE=2 SV=1)

HSP 1 Score: 537.7 bits (1384), Expect = 1.5e-151
Identity = 264/557 (47.40%), Postives = 368/557 (66.07%), Query Frame = 0

Query: 5   TFTMLRICLLAVAL--STVRGEDPYFFFTWNVTYGTISPLGVPQQGILINGQFPGPNINS 64
           T ++L   LL  AL  +     DP+  + + V+Y T SPLGVPQQ I +NGQFPGP +N+
Sbjct: 4   TCSLLASFLLCFALLSAVSFAADPFVSYDFRVSYLTASPLGVPQQVIAVNGQFPGPLLNA 63

Query: 65  TSNNNIVVNVFNNLDEPFLLHWSGVQHRKNSWQDGLLGTNCPIPPGSNFTYHFQVKDQIG 124
           T+N N+VVNVFN+LDEP LL W G+Q R+NSWQDG+LGTNCPIPP  NFTY FQVKDQIG
Sbjct: 64  TTNYNVVVNVFNHLDEPLLLTWPGIQMRRNSWQDGVLGTNCPIPPRWNFTYQFQVKDQIG 123

Query: 125 SFFYYPSTAMHRAGGAFGGLRINSRLLIPVPYADPEDDYTVLAGDWYTKTHSTLKKLLDS 184
           SFFY PS    RA G FG + IN+R +IP+P+  P+ +   + GDWYT+ H  L++ LDS
Sbjct: 124 SFFYSPSLNFQRASGGFGPIVINNRDIIPIPFPQPDGELIFIIGDWYTQDHKALRRALDS 183

Query: 185 GRSIARPDGVLINGK------TAKGDGTDEPLFTMKPGKTYKYRICNVGLKTSLNFRIQG 244
           G+ +  PDGVLINGK      ++  DG D   F ++PGKTY+ R+ NVG+ TSLNFRIQ 
Sbjct: 184 GKELGMPDGVLINGKGPYKYNSSVPDGIDYLTFHVEPGKTYRIRVHNVGISTSLNFRIQN 243

Query: 245 HTMKLVEMEGSHTVQNVYQSLDVHVGQCFSVLVTADQE-PKDYYMVASTRFIKNI----L 304
           H++ LVE EG +T Q  +   DVHVGQ +S LVT DQ+   DYY+VAS RF+       +
Sbjct: 244 HSLLLVETEGHYTSQANFTDFDVHVGQSYSFLVTMDQDATSDYYIVASARFVNETVWQRV 303

Query: 305 VGKAIVRYTNGNSPASP--EIPEAPIGWAWS-LNQFRTFRWNLTASAARPNPQGSYHYGS 364
            G AI+ Y+N   P S    +P+  +   WS ++Q +T R N +AS ARPNPQGS+HYG 
Sbjct: 304 TGVAILHYSNSKGPVSGPLPVPKTDVSSPWSAMSQPKTIRQNTSASGARPNPQGSFHYGQ 363

Query: 365 INITRTIKLLN-SATLVDGKLRYAINDVSHVDPETPLKLAQYFNVADNVFKYDMISDEGS 424
           INIT T  L +   T+++G LR  +N +S V+P TP++LA   N     +K D       
Sbjct: 364 INITNTYILRSLPPTIINGALRATLNGISFVNPSTPVRLADR-NKVKGAYKLDF---PDR 423

Query: 425 VEGRDVTIAPNVVNATYRNFVEIIFENHEKSMQSWHLDGYSFFAVAIEPGRWAPEKRKNY 484
              R + +  +++NATY+ F++++F+N++  +QS+H+DGYSFF V ++ G W+ +K+ +Y
Sbjct: 424 PFNRPLRLDRSMINATYKGFIQVVFQNNDTKIQSFHVDGYSFFVVGMDFGIWSEDKKGSY 483

Query: 485 NLLDAVHRHTIQVFPKSWAAVLVTLDNAGMWNLRSELTENRYLGQQLYFSVLSPANSLRD 544
           N  DA+ R TI+V+P  W AVL++LDN G+WN+R E  +  YLG++ Y  + +P    + 
Sbjct: 484 NNWDAISRSTIEVYPGGWTAVLISLDNVGVWNIRVENLDRWYLGEETYMRITNPEEDGKT 543

BLAST of CmaCh06G009150 vs. ExPASy Swiss-Prot
Match: Q9FHN6 (Monocopper oxidase-like protein SKS2 OS=Arabidopsis thaliana OX=3702 GN=SKS2 PE=2 SV=1)

HSP 1 Score: 530.4 bits (1365), Expect = 2.4e-149
Identity = 257/561 (45.81%), Postives = 367/561 (65.42%), Query Frame = 0

Query: 1   MAGLTFTMLRICLLAVALSTVRGEDPYFFFTWNVTYGTISPLGVPQQGILINGQFPGPNI 60
           MA   F    +   A+        DPY  + + ++Y T SPLGVPQQ I +NG+FPGP I
Sbjct: 1   MAATDFFFAFVFSFALIFGFSFAGDPYVSYDFTLSYITASPLGVPQQVIAVNGKFPGPVI 60

Query: 61  NSTSNNNIVVNVFNNLDEPFLLHWSGVQHRKNSWQDGLLGTNCPIPPGSNFTYHFQVKDQ 120
           N+T+N N+ VNV N+LDEP LL W GVQ R+NSWQDG+LGTNCPIPP  NFTY FQ+KDQ
Sbjct: 61  NATTNYNVHVNVLNHLDEPLLLTWPGVQMRRNSWQDGVLGTNCPIPPNWNFTYDFQLKDQ 120

Query: 121 IGSFFYYPSTAMHRAGGAFGGLRINSRLLIPVPYADPEDDYTVLAGDWYTKTHSTLKKLL 180
           IGS+FY PS    RA G FG L IN+R L+P+P+ +P+ +   + GDWYT+ H+ L+++L
Sbjct: 121 IGSYFYSPSLNFQRASGGFGALIINNRDLVPIPFTEPDGEIIFIIGDWYTQNHTALRRIL 180

Query: 181 DSGRSIARPDGVLINGK------TAKGDGTDEPLFTMKPGKTYKYRICNVGLKTSLNFRI 240
           DSG+ +  PDGVLINGK      ++  DG +     + PGKTY+ R+ NVG+ TSLNFRI
Sbjct: 181 DSGKELGMPDGVLINGKGPFKYNSSVPDGIEHETVNVDPGKTYRIRVHNVGISTSLNFRI 240

Query: 241 QGHTMKLVEMEGSHTVQNVYQSLDVHVGQCFSVLVTADQE-PKDYYMVASTRFIKNI--- 300
           Q H + L+E EG +T Q  +   DVHVGQ +S LVT DQ    DYY+VAS RF+      
Sbjct: 241 QNHKLLLIETEGRYTSQMNFTDFDVHVGQSYSFLVTMDQNATSDYYIVASARFVNETVWQ 300

Query: 301 -LVGKAIVRYTNGNSPASPEIP--EAPIGWAWS-LNQFRTFRWNLTASAARPNPQGSYHY 360
            + G  I+ Y+N   PAS  +P     +   WS +NQ R  + N +AS ARPNPQGS+HY
Sbjct: 301 RVTGVGILHYSNSKGPASGPLPVSATDVNHPWSAMNQPRAIKQNTSASGARPNPQGSFHY 360

Query: 361 GSINITRTIKLLN-SATLVDGKLRYAINDVSHVDPETPLKLAQYFNVADNVFKYDMISD- 420
           G INITRT  L +   T ++GKLR  +N +S V+P TP++LA      D+  K D + D 
Sbjct: 361 GQINITRTYILRSLPPTKINGKLRATLNGISFVNPSTPMRLAD-----DHKVKGDYMLDF 420

Query: 421 -EGSVEGRDVTIAPNVVNATYRNFVEIIFENHEKSMQSWHLDGYSFFAVAIEPGRWAPEK 480
            +  ++ +   ++ +++NATY+ F+++IF+N++  +QS+H+DGY+F+ VA++ G W+ ++
Sbjct: 421 PDRPLDEKLPRLSSSIINATYKGFIQVIFQNNDTKIQSFHIDGYAFYVVAMDFGIWSEDR 480

Query: 481 RKNYNLLDAVHRHTIQVFPKSWAAVLVTLDNAGMWNLRSELTENRYLGQQLYFSVLSPAN 540
             +YN  DAV R T++V+P +W AVL++LDN G+WN+R E  +  YLGQ+ Y  +++P  
Sbjct: 481 NSSYNNWDAVARSTVEVYPGAWTAVLISLDNVGVWNIRVENLDRWYLGQETYMRIINPEE 540

Query: 541 SLRDEYNIPDNTLLCGLVKDM 545
           +   E + P+N + CG ++ M
Sbjct: 541 NGSTEMDPPENVMYCGALQAM 556

BLAST of CmaCh06G009150 vs. ExPASy TrEMBL
Match: A0A6J1IH90 (L-ascorbate oxidase homolog OS=Cucurbita maxima OX=3661 GN=LOC111472953 PE=3 SV=1)

HSP 1 Score: 1156.0 bits (2989), Expect = 0.0e+00
Identity = 552/552 (100.00%), Postives = 552/552 (100.00%), Query Frame = 0

Query: 1   MAGLTFTMLRICLLAVALSTVRGEDPYFFFTWNVTYGTISPLGVPQQGILINGQFPGPNI 60
           MAGLTFTMLRICLLAVALSTVRGEDPYFFFTWNVTYGTISPLGVPQQGILINGQFPGPNI
Sbjct: 1   MAGLTFTMLRICLLAVALSTVRGEDPYFFFTWNVTYGTISPLGVPQQGILINGQFPGPNI 60

Query: 61  NSTSNNNIVVNVFNNLDEPFLLHWSGVQHRKNSWQDGLLGTNCPIPPGSNFTYHFQVKDQ 120
           NSTSNNNIVVNVFNNLDEPFLLHWSGVQHRKNSWQDGLLGTNCPIPPGSNFTYHFQVKDQ
Sbjct: 61  NSTSNNNIVVNVFNNLDEPFLLHWSGVQHRKNSWQDGLLGTNCPIPPGSNFTYHFQVKDQ 120

Query: 121 IGSFFYYPSTAMHRAGGAFGGLRINSRLLIPVPYADPEDDYTVLAGDWYTKTHSTLKKLL 180
           IGSFFYYPSTAMHRAGGAFGGLRINSRLLIPVPYADPEDDYTVLAGDWYTKTHSTLKKLL
Sbjct: 121 IGSFFYYPSTAMHRAGGAFGGLRINSRLLIPVPYADPEDDYTVLAGDWYTKTHSTLKKLL 180

Query: 181 DSGRSIARPDGVLINGKTAKGDGTDEPLFTMKPGKTYKYRICNVGLKTSLNFRIQGHTMK 240
           DSGRSIARPDGVLINGKTAKGDGTDEPLFTMKPGKTYKYRICNVGLKTSLNFRIQGHTMK
Sbjct: 181 DSGRSIARPDGVLINGKTAKGDGTDEPLFTMKPGKTYKYRICNVGLKTSLNFRIQGHTMK 240

Query: 241 LVEMEGSHTVQNVYQSLDVHVGQCFSVLVTADQEPKDYYMVASTRFIKNILVGKAIVRYT 300
           LVEMEGSHTVQNVYQSLDVHVGQCFSVLVTADQEPKDYYMVASTRFIKNILVGKAIVRYT
Sbjct: 241 LVEMEGSHTVQNVYQSLDVHVGQCFSVLVTADQEPKDYYMVASTRFIKNILVGKAIVRYT 300

Query: 301 NGNSPASPEIPEAPIGWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKLLNS 360
           NGNSPASPEIPEAPIGWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKLLNS
Sbjct: 301 NGNSPASPEIPEAPIGWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKLLNS 360

Query: 361 ATLVDGKLRYAINDVSHVDPETPLKLAQYFNVADNVFKYDMISDEGSVEGRDVTIAPNVV 420
           ATLVDGKLRYAINDVSHVDPETPLKLAQYFNVADNVFKYDMISDEGSVEGRDVTIAPNVV
Sbjct: 361 ATLVDGKLRYAINDVSHVDPETPLKLAQYFNVADNVFKYDMISDEGSVEGRDVTIAPNVV 420

Query: 421 NATYRNFVEIIFENHEKSMQSWHLDGYSFFAVAIEPGRWAPEKRKNYNLLDAVHRHTIQV 480
           NATYRNFVEIIFENHEKSMQSWHLDGYSFFAVAIEPGRWAPEKRKNYNLLDAVHRHTIQV
Sbjct: 421 NATYRNFVEIIFENHEKSMQSWHLDGYSFFAVAIEPGRWAPEKRKNYNLLDAVHRHTIQV 480

Query: 481 FPKSWAAVLVTLDNAGMWNLRSELTENRYLGQQLYFSVLSPANSLRDEYNIPDNTLLCGL 540
           FPKSWAAVLVTLDNAGMWNLRSELTENRYLGQQLYFSVLSPANSLRDEYNIPDNTLLCGL
Sbjct: 481 FPKSWAAVLVTLDNAGMWNLRSELTENRYLGQQLYFSVLSPANSLRDEYNIPDNTLLCGL 540

Query: 541 VKDMPLPKPYTI 553
           VKDMPLPKPYTI
Sbjct: 541 VKDMPLPKPYTI 552

BLAST of CmaCh06G009150 vs. ExPASy TrEMBL
Match: A0A6J1FTA8 (L-ascorbate oxidase homolog OS=Cucurbita moschata OX=3662 GN=LOC111447934 PE=3 SV=1)

HSP 1 Score: 1140.2 bits (2948), Expect = 0.0e+00
Identity = 544/552 (98.55%), Postives = 547/552 (99.09%), Query Frame = 0

Query: 1   MAGLTFTMLRICLLAVALSTVRGEDPYFFFTWNVTYGTISPLGVPQQGILINGQFPGPNI 60
           MAGLTFTML ICLLAVALSTVRGEDPYFFFTWNVTYGTISPLGVPQQGILINGQFPGPNI
Sbjct: 1   MAGLTFTMLLICLLAVALSTVRGEDPYFFFTWNVTYGTISPLGVPQQGILINGQFPGPNI 60

Query: 61  NSTSNNNIVVNVFNNLDEPFLLHWSGVQHRKNSWQDGLLGTNCPIPPGSNFTYHFQVKDQ 120
           NSTSNNNIV+NVFNNLDEPFLLHWSGVQHRKNSWQDGLLGTNCPIPPG+NFTYHFQVKDQ
Sbjct: 61  NSTSNNNIVLNVFNNLDEPFLLHWSGVQHRKNSWQDGLLGTNCPIPPGTNFTYHFQVKDQ 120

Query: 121 IGSFFYYPSTAMHRAGGAFGGLRINSRLLIPVPYADPEDDYTVLAGDWYTKTHSTLKKLL 180
           IGSFFYYPSTAMHRAGGAFGGLRINSRLLIPVPYADPEDDYTVLAGDWYTKTHS LKKLL
Sbjct: 121 IGSFFYYPSTAMHRAGGAFGGLRINSRLLIPVPYADPEDDYTVLAGDWYTKTHSALKKLL 180

Query: 181 DSGRSIARPDGVLINGKTAKGDGTDEPLFTMKPGKTYKYRICNVGLKTSLNFRIQGHTMK 240
           DSGRSIARPDGVLINGKTAKGDG+DEPLFTMKPGKTYKYRICNVGLKTSLNFRIQGHTMK
Sbjct: 181 DSGRSIARPDGVLINGKTAKGDGSDEPLFTMKPGKTYKYRICNVGLKTSLNFRIQGHTMK 240

Query: 241 LVEMEGSHTVQNVYQSLDVHVGQCFSVLVTADQEPKDYYMVASTRFIKNILVGKAIVRYT 300
           LVEMEGSHTVQNVYQSLDVHVGQCFSVLVTADQEPKDYYMVASTRFIKNILVGKAIVRYT
Sbjct: 241 LVEMEGSHTVQNVYQSLDVHVGQCFSVLVTADQEPKDYYMVASTRFIKNILVGKAIVRYT 300

Query: 301 NGNSPASPEIPEAPIGWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKLLNS 360
           NGNSPASPEIPEAPIGWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKLLNS
Sbjct: 301 NGNSPASPEIPEAPIGWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKLLNS 360

Query: 361 ATLVDGKLRYAINDVSHVDPETPLKLAQYFNVADNVFKYDMISDEGSVEGRDVTIAPNVV 420
           AT VDGKLRYAIN VSHVDPETPLKLAQYFNVADNVFKYDMISDEGSVEG DVTIAPNVV
Sbjct: 361 ATRVDGKLRYAINGVSHVDPETPLKLAQYFNVADNVFKYDMISDEGSVEGSDVTIAPNVV 420

Query: 421 NATYRNFVEIIFENHEKSMQSWHLDGYSFFAVAIEPGRWAPEKRKNYNLLDAVHRHTIQV 480
           NATYRNFVEIIFENHEKSMQSWHLDGYSFFAVAIEPGRWAPEKRKNYNLLDAVHRHTIQV
Sbjct: 421 NATYRNFVEIIFENHEKSMQSWHLDGYSFFAVAIEPGRWAPEKRKNYNLLDAVHRHTIQV 480

Query: 481 FPKSWAAVLVTLDNAGMWNLRSELTENRYLGQQLYFSVLSPANSLRDEYNIPDNTLLCGL 540
           FPKSWAAVLVTLDNAGMWNLRSELTENRYLGQQLYFSVLSPANSLRDEYNIPDNTLLCGL
Sbjct: 481 FPKSWAAVLVTLDNAGMWNLRSELTENRYLGQQLYFSVLSPANSLRDEYNIPDNTLLCGL 540

Query: 541 VKDMPLPKPYTI 553
           VKDMPLPKPYTI
Sbjct: 541 VKDMPLPKPYTI 552

BLAST of CmaCh06G009150 vs. ExPASy TrEMBL
Match: A0A5A7T1B7 (L-ascorbate oxidase-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold112G00010 PE=3 SV=1)

HSP 1 Score: 1053.5 bits (2723), Expect = 3.0e-304
Identity = 495/553 (89.51%), Postives = 521/553 (94.21%), Query Frame = 0

Query: 1   MAGLTFTMLRICLLAVALSTVRGEDPYFFFTWNVTYGTISPLGVPQQGILINGQFPGPNI 60
           MA LTFT+L   LL  A+ TVRGEDPYFFFTWNVTYGTISPLGVPQQGILINGQFPGPNI
Sbjct: 1   MARLTFTLLLCLLLVAAMPTVRGEDPYFFFTWNVTYGTISPLGVPQQGILINGQFPGPNI 60

Query: 61  NSTSNNNIVVNVFNNLDEPFLLHWSGVQHRKNSWQDGLLGTNCPIPPGSNFTYHFQVKDQ 120
           NST+NNN+V+NVFNNLDEPFLLHWSG+QHRKNSWQDGLLGTNCPIPPG+NFTYHFQVKDQ
Sbjct: 61  NSTTNNNLVINVFNNLDEPFLLHWSGIQHRKNSWQDGLLGTNCPIPPGTNFTYHFQVKDQ 120

Query: 121 IGSFFYYPSTAMHRAGGAFGGLRINSRLLIPVPYADPEDDYTVLAGDWYTKTHSTLKKLL 180
           IGSFFYYPSTAMHRAGGAFGGLR+NSRLLIPVPY DPEDDYTVL GDWYTK++STLKK L
Sbjct: 121 IGSFFYYPSTAMHRAGGAFGGLRVNSRLLIPVPYVDPEDDYTVLIGDWYTKSYSTLKKFL 180

Query: 181 DSGRSIARPDGVLINGKTAKGDGTDEPLFTMKPGKTYKYRICNVGLKTSLNFRIQGHTMK 240
           DSGRSIARPDGVLINGK AKGDGTDEPLFTM PGKTYKYRICNVGLKTSLNFRIQGHTMK
Sbjct: 181 DSGRSIARPDGVLINGKIAKGDGTDEPLFTMNPGKTYKYRICNVGLKTSLNFRIQGHTMK 240

Query: 241 LVEMEGSHTVQNVYQSLDVHVGQCFSVLVTADQEPKDYYMVASTRFIKNILVGKAIVRYT 300
           LVEMEGSHTVQNVYQSLDVHVGQCFSVLVTADQ+P+DYYMVAS+RF KNILVGK IVRYT
Sbjct: 241 LVEMEGSHTVQNVYQSLDVHVGQCFSVLVTADQDPRDYYMVASSRFTKNILVGKGIVRYT 300

Query: 301 NGNSPASPEIPEAPIGWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKLLNS 360
           NG +PASPEIPEAP GWAWSLNQFRTFRWNLTA+AARPNPQGSYHYGSINITRTIK++NS
Sbjct: 301 NGKAPASPEIPEAPRGWAWSLNQFRTFRWNLTANAARPNPQGSYHYGSINITRTIKIVNS 360

Query: 361 ATLVDGKLRYAINDVSHVDPETPLKLAQYFNVADNVFKYDMISDEGSVE-GRDVTIAPNV 420
           AT VDGKLRYAIN VSH+DPETPLKLA+YF VAD VFKYD ISDEGS +    VTIAPNV
Sbjct: 361 ATNVDGKLRYAINGVSHIDPETPLKLAEYFEVADKVFKYDTISDEGSAKTANTVTIAPNV 420

Query: 421 VNATYRNFVEIIFENHEKSMQSWHLDGYSFFAVAIEPGRWAPEKRKNYNLLDAVHRHTIQ 480
           VNAT+RNFVEIIFENHEKS+QSWHLDGYSFFAVAIEPGRW+PEKRKNYNLLDAV RHTIQ
Sbjct: 421 VNATFRNFVEIIFENHEKSLQSWHLDGYSFFAVAIEPGRWSPEKRKNYNLLDAVSRHTIQ 480

Query: 481 VFPKSWAAVLVTLDNAGMWNLRSELTENRYLGQQLYFSVLSPANSLRDEYNIPDNTLLCG 540
           VFPKSW A+L+TLDNAGMWNLRSELTENRYLGQQLYFSV SPA SLRDEYNIPDN LLCG
Sbjct: 481 VFPKSWGAILLTLDNAGMWNLRSELTENRYLGQQLYFSVQSPARSLRDEYNIPDNALLCG 540

Query: 541 LVKDMPLPKPYTI 553
           LVKDM LP+PYTI
Sbjct: 541 LVKDMSLPQPYTI 553

BLAST of CmaCh06G009150 vs. ExPASy TrEMBL
Match: A0A5D3BIA5 (L-ascorbate oxidase-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G00010 PE=3 SV=1)

HSP 1 Score: 1053.1 bits (2722), Expect = 4.0e-304
Identity = 494/553 (89.33%), Postives = 520/553 (94.03%), Query Frame = 0

Query: 1   MAGLTFTMLRICLLAVALSTVRGEDPYFFFTWNVTYGTISPLGVPQQGILINGQFPGPNI 60
           MA LTFT+L   LL  A+ TVRGEDPYFFFTWNVTYGTISPLGVPQQGILINGQFPGPNI
Sbjct: 1   MARLTFTLLLCLLLVAAMPTVRGEDPYFFFTWNVTYGTISPLGVPQQGILINGQFPGPNI 60

Query: 61  NSTSNNNIVVNVFNNLDEPFLLHWSGVQHRKNSWQDGLLGTNCPIPPGSNFTYHFQVKDQ 120
           NST+NNN+V+NVFNNLDEPFLLHWSG+QHRKNSWQDGLLGTNCPIPPG+NFTYHFQVKDQ
Sbjct: 61  NSTTNNNLVINVFNNLDEPFLLHWSGIQHRKNSWQDGLLGTNCPIPPGTNFTYHFQVKDQ 120

Query: 121 IGSFFYYPSTAMHRAGGAFGGLRINSRLLIPVPYADPEDDYTVLAGDWYTKTHSTLKKLL 180
           IGSFFYYPSTAMHRAGGAFGGLR+NSRLLIPVPY DPEDDYTVL GDWYTK+HSTLKK L
Sbjct: 121 IGSFFYYPSTAMHRAGGAFGGLRVNSRLLIPVPYVDPEDDYTVLIGDWYTKSHSTLKKFL 180

Query: 181 DSGRSIARPDGVLINGKTAKGDGTDEPLFTMKPGKTYKYRICNVGLKTSLNFRIQGHTMK 240
           DSGRSIARPDGVLINGK AKGDGTDEPLFTM PGKTYKYRICNVGLK SLNFRIQGHTMK
Sbjct: 181 DSGRSIARPDGVLINGKIAKGDGTDEPLFTMNPGKTYKYRICNVGLKISLNFRIQGHTMK 240

Query: 241 LVEMEGSHTVQNVYQSLDVHVGQCFSVLVTADQEPKDYYMVASTRFIKNILVGKAIVRYT 300
           LVEMEGSHTVQNVYQSLDVHVGQCFSVLVTADQ+P+DYYMVAS+RF KNILVGK IVRYT
Sbjct: 241 LVEMEGSHTVQNVYQSLDVHVGQCFSVLVTADQDPRDYYMVASSRFTKNILVGKGIVRYT 300

Query: 301 NGNSPASPEIPEAPIGWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKLLNS 360
           NG +PASPEIPEAP GWAWSLNQFRTFRWNLTA+AARPNPQGSYHYGSINITRTIK++NS
Sbjct: 301 NGKAPASPEIPEAPRGWAWSLNQFRTFRWNLTANAARPNPQGSYHYGSINITRTIKIVNS 360

Query: 361 ATLVDGKLRYAINDVSHVDPETPLKLAQYFNVADNVFKYDMISDEGSVE-GRDVTIAPNV 420
           AT +DGKLRYAIN VSH+DPETPLKLA+YF VAD VFKYD ISDEGS +    VTIAPNV
Sbjct: 361 ATNIDGKLRYAINGVSHIDPETPLKLAEYFEVADKVFKYDTISDEGSAKTANTVTIAPNV 420

Query: 421 VNATYRNFVEIIFENHEKSMQSWHLDGYSFFAVAIEPGRWAPEKRKNYNLLDAVHRHTIQ 480
           VNAT+RNFVEIIFENHEKS+QSWHLDGYSFFAVAIEPGRW+PEKRKNYNLLDAV RHTIQ
Sbjct: 421 VNATFRNFVEIIFENHEKSLQSWHLDGYSFFAVAIEPGRWSPEKRKNYNLLDAVSRHTIQ 480

Query: 481 VFPKSWAAVLVTLDNAGMWNLRSELTENRYLGQQLYFSVLSPANSLRDEYNIPDNTLLCG 540
           VFPKSW A+L+TLDNAGMWNLRSELTENRYLGQQLYFSV SPA SLRDEYNIPDN LLCG
Sbjct: 481 VFPKSWGAILLTLDNAGMWNLRSELTENRYLGQQLYFSVQSPARSLRDEYNIPDNALLCG 540

Query: 541 LVKDMPLPKPYTI 553
           LVKDM LP+PYTI
Sbjct: 541 LVKDMSLPQPYTI 553

BLAST of CmaCh06G009150 vs. ExPASy TrEMBL
Match: A0A1S3AX96 (L-ascorbate oxidase homolog OS=Cucumis melo OX=3656 GN=LOC103483330 PE=3 SV=1)

HSP 1 Score: 1053.1 bits (2722), Expect = 4.0e-304
Identity = 494/553 (89.33%), Postives = 520/553 (94.03%), Query Frame = 0

Query: 1   MAGLTFTMLRICLLAVALSTVRGEDPYFFFTWNVTYGTISPLGVPQQGILINGQFPGPNI 60
           MA LTFT+L   LL  A+ TVRGEDPYFFFTWNVTYGTISPLGVPQQGILINGQFPGPNI
Sbjct: 1   MARLTFTLLLCLLLVAAMPTVRGEDPYFFFTWNVTYGTISPLGVPQQGILINGQFPGPNI 60

Query: 61  NSTSNNNIVVNVFNNLDEPFLLHWSGVQHRKNSWQDGLLGTNCPIPPGSNFTYHFQVKDQ 120
           NST+NNN+V+NVFNNLDEPFLLHWSG+QHRKNSWQDGLLGTNCPIPPG+NFTYHFQVKDQ
Sbjct: 61  NSTTNNNLVINVFNNLDEPFLLHWSGIQHRKNSWQDGLLGTNCPIPPGTNFTYHFQVKDQ 120

Query: 121 IGSFFYYPSTAMHRAGGAFGGLRINSRLLIPVPYADPEDDYTVLAGDWYTKTHSTLKKLL 180
           IGSFFYYPSTAMHRAGGAFGGLR+NSRLLIPVPY DPEDDYTVL GDWYTK+HSTLKK L
Sbjct: 121 IGSFFYYPSTAMHRAGGAFGGLRVNSRLLIPVPYVDPEDDYTVLIGDWYTKSHSTLKKFL 180

Query: 181 DSGRSIARPDGVLINGKTAKGDGTDEPLFTMKPGKTYKYRICNVGLKTSLNFRIQGHTMK 240
           DSGRSIARPDGVLINGK AKGDGTDEPLFTM PGKTYKYRICNVGLK SLNFRIQGHTMK
Sbjct: 181 DSGRSIARPDGVLINGKIAKGDGTDEPLFTMNPGKTYKYRICNVGLKISLNFRIQGHTMK 240

Query: 241 LVEMEGSHTVQNVYQSLDVHVGQCFSVLVTADQEPKDYYMVASTRFIKNILVGKAIVRYT 300
           LVEMEGSHTVQNVYQSLDVHVGQCFSVLVTADQ+P+DYYMVAS+RF KNILVGK IVRYT
Sbjct: 241 LVEMEGSHTVQNVYQSLDVHVGQCFSVLVTADQDPRDYYMVASSRFTKNILVGKGIVRYT 300

Query: 301 NGNSPASPEIPEAPIGWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKLLNS 360
           NG +PASPEIPEAP GWAWSLNQFRTFRWNLTA+AARPNPQGSYHYGSINITRTIK++NS
Sbjct: 301 NGKAPASPEIPEAPRGWAWSLNQFRTFRWNLTANAARPNPQGSYHYGSINITRTIKIVNS 360

Query: 361 ATLVDGKLRYAINDVSHVDPETPLKLAQYFNVADNVFKYDMISDEGSVE-GRDVTIAPNV 420
           AT +DGKLRYAIN VSH+DPETPLKLA+YF VAD VFKYD ISDEGS +    VTIAPNV
Sbjct: 361 ATNIDGKLRYAINGVSHIDPETPLKLAEYFEVADKVFKYDTISDEGSAKTANTVTIAPNV 420

Query: 421 VNATYRNFVEIIFENHEKSMQSWHLDGYSFFAVAIEPGRWAPEKRKNYNLLDAVHRHTIQ 480
           VNAT+RNFVEIIFENHEKS+QSWHLDGYSFFAVAIEPGRW+PEKRKNYNLLDAV RHTIQ
Sbjct: 421 VNATFRNFVEIIFENHEKSLQSWHLDGYSFFAVAIEPGRWSPEKRKNYNLLDAVSRHTIQ 480

Query: 481 VFPKSWAAVLVTLDNAGMWNLRSELTENRYLGQQLYFSVLSPANSLRDEYNIPDNTLLCG 540
           VFPKSW A+L+TLDNAGMWNLRSELTENRYLGQQLYFSV SPA SLRDEYNIPDN LLCG
Sbjct: 481 VFPKSWGAILLTLDNAGMWNLRSELTENRYLGQQLYFSVQSPARSLRDEYNIPDNALLCG 540

Query: 541 LVKDMPLPKPYTI 553
           LVKDM LP+PYTI
Sbjct: 541 LVKDMSLPQPYTI 553

BLAST of CmaCh06G009150 vs. NCBI nr
Match: XP_022974314.1 (L-ascorbate oxidase homolog [Cucurbita maxima])

HSP 1 Score: 1156.0 bits (2989), Expect = 0.0e+00
Identity = 552/552 (100.00%), Postives = 552/552 (100.00%), Query Frame = 0

Query: 1   MAGLTFTMLRICLLAVALSTVRGEDPYFFFTWNVTYGTISPLGVPQQGILINGQFPGPNI 60
           MAGLTFTMLRICLLAVALSTVRGEDPYFFFTWNVTYGTISPLGVPQQGILINGQFPGPNI
Sbjct: 1   MAGLTFTMLRICLLAVALSTVRGEDPYFFFTWNVTYGTISPLGVPQQGILINGQFPGPNI 60

Query: 61  NSTSNNNIVVNVFNNLDEPFLLHWSGVQHRKNSWQDGLLGTNCPIPPGSNFTYHFQVKDQ 120
           NSTSNNNIVVNVFNNLDEPFLLHWSGVQHRKNSWQDGLLGTNCPIPPGSNFTYHFQVKDQ
Sbjct: 61  NSTSNNNIVVNVFNNLDEPFLLHWSGVQHRKNSWQDGLLGTNCPIPPGSNFTYHFQVKDQ 120

Query: 121 IGSFFYYPSTAMHRAGGAFGGLRINSRLLIPVPYADPEDDYTVLAGDWYTKTHSTLKKLL 180
           IGSFFYYPSTAMHRAGGAFGGLRINSRLLIPVPYADPEDDYTVLAGDWYTKTHSTLKKLL
Sbjct: 121 IGSFFYYPSTAMHRAGGAFGGLRINSRLLIPVPYADPEDDYTVLAGDWYTKTHSTLKKLL 180

Query: 181 DSGRSIARPDGVLINGKTAKGDGTDEPLFTMKPGKTYKYRICNVGLKTSLNFRIQGHTMK 240
           DSGRSIARPDGVLINGKTAKGDGTDEPLFTMKPGKTYKYRICNVGLKTSLNFRIQGHTMK
Sbjct: 181 DSGRSIARPDGVLINGKTAKGDGTDEPLFTMKPGKTYKYRICNVGLKTSLNFRIQGHTMK 240

Query: 241 LVEMEGSHTVQNVYQSLDVHVGQCFSVLVTADQEPKDYYMVASTRFIKNILVGKAIVRYT 300
           LVEMEGSHTVQNVYQSLDVHVGQCFSVLVTADQEPKDYYMVASTRFIKNILVGKAIVRYT
Sbjct: 241 LVEMEGSHTVQNVYQSLDVHVGQCFSVLVTADQEPKDYYMVASTRFIKNILVGKAIVRYT 300

Query: 301 NGNSPASPEIPEAPIGWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKLLNS 360
           NGNSPASPEIPEAPIGWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKLLNS
Sbjct: 301 NGNSPASPEIPEAPIGWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKLLNS 360

Query: 361 ATLVDGKLRYAINDVSHVDPETPLKLAQYFNVADNVFKYDMISDEGSVEGRDVTIAPNVV 420
           ATLVDGKLRYAINDVSHVDPETPLKLAQYFNVADNVFKYDMISDEGSVEGRDVTIAPNVV
Sbjct: 361 ATLVDGKLRYAINDVSHVDPETPLKLAQYFNVADNVFKYDMISDEGSVEGRDVTIAPNVV 420

Query: 421 NATYRNFVEIIFENHEKSMQSWHLDGYSFFAVAIEPGRWAPEKRKNYNLLDAVHRHTIQV 480
           NATYRNFVEIIFENHEKSMQSWHLDGYSFFAVAIEPGRWAPEKRKNYNLLDAVHRHTIQV
Sbjct: 421 NATYRNFVEIIFENHEKSMQSWHLDGYSFFAVAIEPGRWAPEKRKNYNLLDAVHRHTIQV 480

Query: 481 FPKSWAAVLVTLDNAGMWNLRSELTENRYLGQQLYFSVLSPANSLRDEYNIPDNTLLCGL 540
           FPKSWAAVLVTLDNAGMWNLRSELTENRYLGQQLYFSVLSPANSLRDEYNIPDNTLLCGL
Sbjct: 481 FPKSWAAVLVTLDNAGMWNLRSELTENRYLGQQLYFSVLSPANSLRDEYNIPDNTLLCGL 540

Query: 541 VKDMPLPKPYTI 553
           VKDMPLPKPYTI
Sbjct: 541 VKDMPLPKPYTI 552

BLAST of CmaCh06G009150 vs. NCBI nr
Match: XP_022943104.1 (L-ascorbate oxidase homolog [Cucurbita moschata])

HSP 1 Score: 1140.2 bits (2948), Expect = 0.0e+00
Identity = 544/552 (98.55%), Postives = 547/552 (99.09%), Query Frame = 0

Query: 1   MAGLTFTMLRICLLAVALSTVRGEDPYFFFTWNVTYGTISPLGVPQQGILINGQFPGPNI 60
           MAGLTFTML ICLLAVALSTVRGEDPYFFFTWNVTYGTISPLGVPQQGILINGQFPGPNI
Sbjct: 1   MAGLTFTMLLICLLAVALSTVRGEDPYFFFTWNVTYGTISPLGVPQQGILINGQFPGPNI 60

Query: 61  NSTSNNNIVVNVFNNLDEPFLLHWSGVQHRKNSWQDGLLGTNCPIPPGSNFTYHFQVKDQ 120
           NSTSNNNIV+NVFNNLDEPFLLHWSGVQHRKNSWQDGLLGTNCPIPPG+NFTYHFQVKDQ
Sbjct: 61  NSTSNNNIVLNVFNNLDEPFLLHWSGVQHRKNSWQDGLLGTNCPIPPGTNFTYHFQVKDQ 120

Query: 121 IGSFFYYPSTAMHRAGGAFGGLRINSRLLIPVPYADPEDDYTVLAGDWYTKTHSTLKKLL 180
           IGSFFYYPSTAMHRAGGAFGGLRINSRLLIPVPYADPEDDYTVLAGDWYTKTHS LKKLL
Sbjct: 121 IGSFFYYPSTAMHRAGGAFGGLRINSRLLIPVPYADPEDDYTVLAGDWYTKTHSALKKLL 180

Query: 181 DSGRSIARPDGVLINGKTAKGDGTDEPLFTMKPGKTYKYRICNVGLKTSLNFRIQGHTMK 240
           DSGRSIARPDGVLINGKTAKGDG+DEPLFTMKPGKTYKYRICNVGLKTSLNFRIQGHTMK
Sbjct: 181 DSGRSIARPDGVLINGKTAKGDGSDEPLFTMKPGKTYKYRICNVGLKTSLNFRIQGHTMK 240

Query: 241 LVEMEGSHTVQNVYQSLDVHVGQCFSVLVTADQEPKDYYMVASTRFIKNILVGKAIVRYT 300
           LVEMEGSHTVQNVYQSLDVHVGQCFSVLVTADQEPKDYYMVASTRFIKNILVGKAIVRYT
Sbjct: 241 LVEMEGSHTVQNVYQSLDVHVGQCFSVLVTADQEPKDYYMVASTRFIKNILVGKAIVRYT 300

Query: 301 NGNSPASPEIPEAPIGWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKLLNS 360
           NGNSPASPEIPEAPIGWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKLLNS
Sbjct: 301 NGNSPASPEIPEAPIGWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKLLNS 360

Query: 361 ATLVDGKLRYAINDVSHVDPETPLKLAQYFNVADNVFKYDMISDEGSVEGRDVTIAPNVV 420
           AT VDGKLRYAIN VSHVDPETPLKLAQYFNVADNVFKYDMISDEGSVEG DVTIAPNVV
Sbjct: 361 ATRVDGKLRYAINGVSHVDPETPLKLAQYFNVADNVFKYDMISDEGSVEGSDVTIAPNVV 420

Query: 421 NATYRNFVEIIFENHEKSMQSWHLDGYSFFAVAIEPGRWAPEKRKNYNLLDAVHRHTIQV 480
           NATYRNFVEIIFENHEKSMQSWHLDGYSFFAVAIEPGRWAPEKRKNYNLLDAVHRHTIQV
Sbjct: 421 NATYRNFVEIIFENHEKSMQSWHLDGYSFFAVAIEPGRWAPEKRKNYNLLDAVHRHTIQV 480

Query: 481 FPKSWAAVLVTLDNAGMWNLRSELTENRYLGQQLYFSVLSPANSLRDEYNIPDNTLLCGL 540
           FPKSWAAVLVTLDNAGMWNLRSELTENRYLGQQLYFSVLSPANSLRDEYNIPDNTLLCGL
Sbjct: 481 FPKSWAAVLVTLDNAGMWNLRSELTENRYLGQQLYFSVLSPANSLRDEYNIPDNTLLCGL 540

Query: 541 VKDMPLPKPYTI 553
           VKDMPLPKPYTI
Sbjct: 541 VKDMPLPKPYTI 552

BLAST of CmaCh06G009150 vs. NCBI nr
Match: KAG6596990.1 (L-ascorbate oxidase-like protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1137.5 bits (2941), Expect = 0.0e+00
Identity = 543/552 (98.37%), Postives = 546/552 (98.91%), Query Frame = 0

Query: 1   MAGLTFTMLRICLLAVALSTVRGEDPYFFFTWNVTYGTISPLGVPQQGILINGQFPGPNI 60
           MAGLTFTML ICLLAVALSTVRGEDPYF+FTWNVTYGTISPLGVPQQGILINGQFPGPNI
Sbjct: 1   MAGLTFTMLLICLLAVALSTVRGEDPYFYFTWNVTYGTISPLGVPQQGILINGQFPGPNI 60

Query: 61  NSTSNNNIVVNVFNNLDEPFLLHWSGVQHRKNSWQDGLLGTNCPIPPGSNFTYHFQVKDQ 120
           NSTSNNNIVVNVFNNLDEPFLLHWSGVQHRKNSWQDGLLGTNCPIPPG+NFTYHFQVKDQ
Sbjct: 61  NSTSNNNIVVNVFNNLDEPFLLHWSGVQHRKNSWQDGLLGTNCPIPPGTNFTYHFQVKDQ 120

Query: 121 IGSFFYYPSTAMHRAGGAFGGLRINSRLLIPVPYADPEDDYTVLAGDWYTKTHSTLKKLL 180
           IGSFFYYPSTAMHRAGGAFGGLRINSRLLIPVPYADPEDDYTVLAGDWYTKTHS LKKLL
Sbjct: 121 IGSFFYYPSTAMHRAGGAFGGLRINSRLLIPVPYADPEDDYTVLAGDWYTKTHSALKKLL 180

Query: 181 DSGRSIARPDGVLINGKTAKGDGTDEPLFTMKPGKTYKYRICNVGLKTSLNFRIQGHTMK 240
           DSGRSIARPDGVLINGKTAKGDG+DEPLFTMKPGKTYKYRICNVGLKTSLNFRIQGHTMK
Sbjct: 181 DSGRSIARPDGVLINGKTAKGDGSDEPLFTMKPGKTYKYRICNVGLKTSLNFRIQGHTMK 240

Query: 241 LVEMEGSHTVQNVYQSLDVHVGQCFSVLVTADQEPKDYYMVASTRFIKNILVGKAIVRYT 300
           LVEMEGSHTVQNVYQSLDVHVGQCFSVLVTADQEPKDYYMVASTRFIKNILVGKAIVRYT
Sbjct: 241 LVEMEGSHTVQNVYQSLDVHVGQCFSVLVTADQEPKDYYMVASTRFIKNILVGKAIVRYT 300

Query: 301 NGNSPASPEIPEAPIGWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKLLNS 360
           NGNSPASPEIPEAPIGWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKLLNS
Sbjct: 301 NGNSPASPEIPEAPIGWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKLLNS 360

Query: 361 ATLVDGKLRYAINDVSHVDPETPLKLAQYFNVADNVFKYDMISDEGSVEGRDVTIAPNVV 420
           AT VDGKLRYAIN VSHVDPETPLKLAQYFNVADNVFKYDMISDEG VEG DVTIAPNVV
Sbjct: 361 ATRVDGKLRYAINGVSHVDPETPLKLAQYFNVADNVFKYDMISDEGWVEGSDVTIAPNVV 420

Query: 421 NATYRNFVEIIFENHEKSMQSWHLDGYSFFAVAIEPGRWAPEKRKNYNLLDAVHRHTIQV 480
           NATYRNFVEIIFENHEKSMQSWHLDGYSFFAVAIEPGRWAPEKRKNYNLLDAVHRHTIQV
Sbjct: 421 NATYRNFVEIIFENHEKSMQSWHLDGYSFFAVAIEPGRWAPEKRKNYNLLDAVHRHTIQV 480

Query: 481 FPKSWAAVLVTLDNAGMWNLRSELTENRYLGQQLYFSVLSPANSLRDEYNIPDNTLLCGL 540
           FPKSWAAVLVTLDNAGMWNLRSELTENRYLGQQLYFSVLSPANSLRDEYNIPDNTLLCGL
Sbjct: 481 FPKSWAAVLVTLDNAGMWNLRSELTENRYLGQQLYFSVLSPANSLRDEYNIPDNTLLCGL 540

Query: 541 VKDMPLPKPYTI 553
           VKDMPLPKPYTI
Sbjct: 541 VKDMPLPKPYTI 552

BLAST of CmaCh06G009150 vs. NCBI nr
Match: XP_023539597.1 (L-ascorbate oxidase homolog [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1134.4 bits (2933), Expect = 0.0e+00
Identity = 539/552 (97.64%), Postives = 546/552 (98.91%), Query Frame = 0

Query: 1   MAGLTFTMLRICLLAVALSTVRGEDPYFFFTWNVTYGTISPLGVPQQGILINGQFPGPNI 60
           MAGLTFTML IC+LAVALSTVRGEDPYFFFTWNVTYGTISPLGVPQQGILINGQFPGPNI
Sbjct: 1   MAGLTFTMLLICVLAVALSTVRGEDPYFFFTWNVTYGTISPLGVPQQGILINGQFPGPNI 60

Query: 61  NSTSNNNIVVNVFNNLDEPFLLHWSGVQHRKNSWQDGLLGTNCPIPPGSNFTYHFQVKDQ 120
           NSTSNNNIVVNVFNNLDEPFLLHWSGVQHRKNSWQDGLLGTNCPIPPG+NFTYHFQVKDQ
Sbjct: 61  NSTSNNNIVVNVFNNLDEPFLLHWSGVQHRKNSWQDGLLGTNCPIPPGTNFTYHFQVKDQ 120

Query: 121 IGSFFYYPSTAMHRAGGAFGGLRINSRLLIPVPYADPEDDYTVLAGDWYTKTHSTLKKLL 180
           IGSFFYYPSTA+HRAGGAFGGLRINSRLLIPVPYADPEDDYTVLAGDWYT THSTLKKLL
Sbjct: 121 IGSFFYYPSTALHRAGGAFGGLRINSRLLIPVPYADPEDDYTVLAGDWYTNTHSTLKKLL 180

Query: 181 DSGRSIARPDGVLINGKTAKGDGTDEPLFTMKPGKTYKYRICNVGLKTSLNFRIQGHTMK 240
           DSGRSIARPDGVLINGKTAKGDG+DEPLFTMKPGKTYKYRICNVGLKTSLNFRIQGHTMK
Sbjct: 181 DSGRSIARPDGVLINGKTAKGDGSDEPLFTMKPGKTYKYRICNVGLKTSLNFRIQGHTMK 240

Query: 241 LVEMEGSHTVQNVYQSLDVHVGQCFSVLVTADQEPKDYYMVASTRFIKNILVGKAIVRYT 300
           LVEMEGSHTVQNVYQSLDVHVGQCFSVLVTADQEPKDYYMVASTRFIKNILVGKAIVRYT
Sbjct: 241 LVEMEGSHTVQNVYQSLDVHVGQCFSVLVTADQEPKDYYMVASTRFIKNILVGKAIVRYT 300

Query: 301 NGNSPASPEIPEAPIGWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKLLNS 360
           NGNSP SPEIPEAPIGWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKLLNS
Sbjct: 301 NGNSPVSPEIPEAPIGWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKLLNS 360

Query: 361 ATLVDGKLRYAINDVSHVDPETPLKLAQYFNVADNVFKYDMISDEGSVEGRDVTIAPNVV 420
           AT VDGKLRYAIN VSHVDPETPLKLAQYFNVADNVFKYDMISDEGSVEG DVTIAPNVV
Sbjct: 361 ATRVDGKLRYAINGVSHVDPETPLKLAQYFNVADNVFKYDMISDEGSVEGSDVTIAPNVV 420

Query: 421 NATYRNFVEIIFENHEKSMQSWHLDGYSFFAVAIEPGRWAPEKRKNYNLLDAVHRHTIQV 480
           NATYRNFVEI+FENHEKS+QSWHLDGYSFFAVAIEPGRW+PEKRKNYNLLDAVHRHTIQV
Sbjct: 421 NATYRNFVEIVFENHEKSVQSWHLDGYSFFAVAIEPGRWSPEKRKNYNLLDAVHRHTIQV 480

Query: 481 FPKSWAAVLVTLDNAGMWNLRSELTENRYLGQQLYFSVLSPANSLRDEYNIPDNTLLCGL 540
           FPKSWAAVLVTLDNAGMWNLRSELTENRYLGQQLYFSVLSPANSLRDEYNIPDNTLLCGL
Sbjct: 481 FPKSWAAVLVTLDNAGMWNLRSELTENRYLGQQLYFSVLSPANSLRDEYNIPDNTLLCGL 540

Query: 541 VKDMPLPKPYTI 553
           VKDMPLPKPYTI
Sbjct: 541 VKDMPLPKPYTI 552

BLAST of CmaCh06G009150 vs. NCBI nr
Match: KAA0036016.1 (L-ascorbate oxidase-like protein [Cucumis melo var. makuwa])

HSP 1 Score: 1053.5 bits (2723), Expect = 6.3e-304
Identity = 495/553 (89.51%), Postives = 521/553 (94.21%), Query Frame = 0

Query: 1   MAGLTFTMLRICLLAVALSTVRGEDPYFFFTWNVTYGTISPLGVPQQGILINGQFPGPNI 60
           MA LTFT+L   LL  A+ TVRGEDPYFFFTWNVTYGTISPLGVPQQGILINGQFPGPNI
Sbjct: 1   MARLTFTLLLCLLLVAAMPTVRGEDPYFFFTWNVTYGTISPLGVPQQGILINGQFPGPNI 60

Query: 61  NSTSNNNIVVNVFNNLDEPFLLHWSGVQHRKNSWQDGLLGTNCPIPPGSNFTYHFQVKDQ 120
           NST+NNN+V+NVFNNLDEPFLLHWSG+QHRKNSWQDGLLGTNCPIPPG+NFTYHFQVKDQ
Sbjct: 61  NSTTNNNLVINVFNNLDEPFLLHWSGIQHRKNSWQDGLLGTNCPIPPGTNFTYHFQVKDQ 120

Query: 121 IGSFFYYPSTAMHRAGGAFGGLRINSRLLIPVPYADPEDDYTVLAGDWYTKTHSTLKKLL 180
           IGSFFYYPSTAMHRAGGAFGGLR+NSRLLIPVPY DPEDDYTVL GDWYTK++STLKK L
Sbjct: 121 IGSFFYYPSTAMHRAGGAFGGLRVNSRLLIPVPYVDPEDDYTVLIGDWYTKSYSTLKKFL 180

Query: 181 DSGRSIARPDGVLINGKTAKGDGTDEPLFTMKPGKTYKYRICNVGLKTSLNFRIQGHTMK 240
           DSGRSIARPDGVLINGK AKGDGTDEPLFTM PGKTYKYRICNVGLKTSLNFRIQGHTMK
Sbjct: 181 DSGRSIARPDGVLINGKIAKGDGTDEPLFTMNPGKTYKYRICNVGLKTSLNFRIQGHTMK 240

Query: 241 LVEMEGSHTVQNVYQSLDVHVGQCFSVLVTADQEPKDYYMVASTRFIKNILVGKAIVRYT 300
           LVEMEGSHTVQNVYQSLDVHVGQCFSVLVTADQ+P+DYYMVAS+RF KNILVGK IVRYT
Sbjct: 241 LVEMEGSHTVQNVYQSLDVHVGQCFSVLVTADQDPRDYYMVASSRFTKNILVGKGIVRYT 300

Query: 301 NGNSPASPEIPEAPIGWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKLLNS 360
           NG +PASPEIPEAP GWAWSLNQFRTFRWNLTA+AARPNPQGSYHYGSINITRTIK++NS
Sbjct: 301 NGKAPASPEIPEAPRGWAWSLNQFRTFRWNLTANAARPNPQGSYHYGSINITRTIKIVNS 360

Query: 361 ATLVDGKLRYAINDVSHVDPETPLKLAQYFNVADNVFKYDMISDEGSVE-GRDVTIAPNV 420
           AT VDGKLRYAIN VSH+DPETPLKLA+YF VAD VFKYD ISDEGS +    VTIAPNV
Sbjct: 361 ATNVDGKLRYAINGVSHIDPETPLKLAEYFEVADKVFKYDTISDEGSAKTANTVTIAPNV 420

Query: 421 VNATYRNFVEIIFENHEKSMQSWHLDGYSFFAVAIEPGRWAPEKRKNYNLLDAVHRHTIQ 480
           VNAT+RNFVEIIFENHEKS+QSWHLDGYSFFAVAIEPGRW+PEKRKNYNLLDAV RHTIQ
Sbjct: 421 VNATFRNFVEIIFENHEKSLQSWHLDGYSFFAVAIEPGRWSPEKRKNYNLLDAVSRHTIQ 480

Query: 481 VFPKSWAAVLVTLDNAGMWNLRSELTENRYLGQQLYFSVLSPANSLRDEYNIPDNTLLCG 540
           VFPKSW A+L+TLDNAGMWNLRSELTENRYLGQQLYFSV SPA SLRDEYNIPDN LLCG
Sbjct: 481 VFPKSWGAILLTLDNAGMWNLRSELTENRYLGQQLYFSVQSPARSLRDEYNIPDNALLCG 540

Query: 541 LVKDMPLPKPYTI 553
           LVKDM LP+PYTI
Sbjct: 541 LVKDMSLPQPYTI 553

BLAST of CmaCh06G009150 vs. TAIR 10
Match: AT3G13390.1 (SKU5 similar 11 )

HSP 1 Score: 883.2 bits (2281), Expect = 1.0e-256
Identity = 398/551 (72.23%), Postives = 462/551 (83.85%), Query Frame = 0

Query: 1   MAGLTFTMLRICLLAVALSTVRGEDPYFFFTWNVTYGTISPLGVPQQGILINGQFPGPNI 60
           M G+      + L A A   VR EDPYF   WNVTYGT+SPLGVPQQ ILINGQFPGPN+
Sbjct: 1   MRGVKLLAACLYLAAAATVVVRAEDPYFHHVWNVTYGTVSPLGVPQQVILINGQFPGPNV 60

Query: 61  NSTSNNNIVVNVFNNLDEPFLLHWSGVQHRKNSWQDGLLGTNCPIPPGSNFTYHFQVKDQ 120
           NSTSNNN+++NVFNNLDEPFLL W+G+QHRKN WQDG  GT CPI PG+N+TYHFQ KDQ
Sbjct: 61  NSTSNNNVIINVFNNLDEPFLLTWNGIQHRKNCWQDGTPGTMCPIMPGTNYTYHFQPKDQ 120

Query: 121 IGSFFYYPSTAMHRAGGAFGGLRINSRLLIPVPYADPEDDYTVLAGDWYTKTHSTLKKLL 180
           IGS+FYYPSTAMHR+ G FGGLR+NSRLLIPVPYADPEDDYTVL GDWYTK+H+ LKK L
Sbjct: 121 IGSYFYYPSTAMHRSAGGFGGLRVNSRLLIPVPYADPEDDYTVLIGDWYTKSHTQLKKFL 180

Query: 181 DSGRSIARPDGVLINGKTAKGDGTDEPLFTMKPGKTYKYRICNVGLKTSLNFRIQGHTMK 240
           DSGR++ RPDG+LINGK+ KGDG+D PLFT+KPGKTY+ RICNVGLKTSLNFRIQ H +K
Sbjct: 181 DSGRTLGRPDGILINGKSGKGDGSDAPLFTLKPGKTYRVRICNVGLKTSLNFRIQNHKLK 240

Query: 241 LVEMEGSHTVQNVYQSLDVHVGQCFSVLVTADQEPKDYYMVASTRFIKNILVGKAIVRYT 300
           LVEMEGSH +QN Y SLDVHVGQC+  ++TA+QE KDYYMVAS+RF+K+++    ++RY 
Sbjct: 241 LVEMEGSHVLQNDYDSLDVHVGQCYGTILTANQEAKDYYMVASSRFLKSVITTTGLLRYE 300

Query: 301 NGNSPASPEIPEAPIGWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKLLNS 360
            G  PAS ++P  P+GWAWSLNQFR+FRWNLTASAARPNPQGSYHYG INITRTIKL+N+
Sbjct: 301 GGKGPASSQLPPGPVGWAWSLNQFRSFRWNLTASAARPNPQGSYHYGKINITRTIKLVNT 360

Query: 361 ATLVDGKLRYAINDVSHVDPETPLKLAQYFNVADNVFKYDMISDEGSVEG-RDVTIAPNV 420
              VDGKLRYA+N VSH DPETPLKLA+YF VAD VFKYD I+D  + E  + + I PNV
Sbjct: 361 QGKVDGKLRYALNGVSHTDPETPLKLAEYFGVADKVFKYDSITDNPTPEQIKSIKIVPNV 420

Query: 421 VNATYRNFVEIIFENHEKSMQSWHLDGYSFFAVAIEPGRWAPEKRKNYNLLDAVHRHTIQ 480
           +N T+R F+E++FENHEKS+QSWHLDGYSFFAVA+EPG W PEKRKNYNLLDAV RHT+Q
Sbjct: 421 LNITHRTFIEVVFENHEKSVQSWHLDGYSFFAVAVEPGTWTPEKRKNYNLLDAVSRHTVQ 480

Query: 481 VFPKSWAAVLVTLDNAGMWNLRSELTENRYLGQQLYFSVLSPANSLRDEYNIPDNTLLCG 540
           V+PK WAA+L+T DN GMWN+RSE +E RYLGQQLY SVLSP  SLRDEYN+P+ +L CG
Sbjct: 481 VYPKCWAAILLTFDNCGMWNVRSENSERRYLGQQLYASVLSPEKSLRDEYNMPETSLQCG 540

Query: 541 LVKDMPLPKPY 551
           LVK  P P PY
Sbjct: 541 LVKGTPKPNPY 551

BLAST of CmaCh06G009150 vs. TAIR 10
Match: AT1G55570.1 (SKU5 similar 12 )

HSP 1 Score: 882.9 bits (2280), Expect = 1.4e-256
Identity = 398/549 (72.50%), Postives = 459/549 (83.61%), Query Frame = 0

Query: 3   GLTFTMLRICLLAVALSTVRGEDPYFFFTWNVTYGTISPLGVPQQGILINGQFPGPNINS 62
           G+    + +C+    +  V+ EDPYF   WNVTYGT SPLGVPQQ ILINGQFPGPNINS
Sbjct: 4   GVKLLAVCLCVATATVMMVQAEDPYFHHVWNVTYGTASPLGVPQQVILINGQFPGPNINS 63

Query: 63  TSNNNIVVNVFNNLDEPFLLHWSGVQHRKNSWQDGLLGTNCPIPPGSNFTYHFQVKDQIG 122
           TSNNN++VNVFNNLDEPFL+ W+G+QHRKN WQDG  GT CPIPPG NFTYHFQ KDQIG
Sbjct: 64  TSNNNVIVNVFNNLDEPFLITWAGIQHRKNCWQDGTAGTMCPIPPGQNFTYHFQPKDQIG 123

Query: 123 SFFYYPSTAMHRAGGAFGGLRINSRLLIPVPYADPEDDYTVLAGDWYTKTHSTLKKLLDS 182
           S+FYYP+TAMHRA G FGGLR+NSRLLIPVPYADPEDDYT+L  DWYTK+H+ LKK LDS
Sbjct: 124 SYFYYPTTAMHRAAGGFGGLRVNSRLLIPVPYADPEDDYTILINDWYTKSHTQLKKFLDS 183

Query: 183 GRSIARPDGVLINGKTAKGDGTDEPLFTMKPGKTYKYRICNVGLKTSLNFRIQGHTMKLV 242
           GR+I RPDG+LINGK+ K DG+D+PLFT+KPGKTY+ RICNVGLK SLNFRIQ H MKLV
Sbjct: 184 GRTIGRPDGILINGKSGKTDGSDKPLFTLKPGKTYRVRICNVGLKASLNFRIQNHKMKLV 243

Query: 243 EMEGSHTVQNVYQSLDVHVGQCFSVLVTADQEPKDYYMVASTRFIKNILVGKAIVRYTNG 302
           EMEGSH +QN Y SLDVHVGQCF V+VTADQEPKDYYM+ASTRF+K  L    ++RY  G
Sbjct: 244 EMEGSHVLQNDYDSLDVHVGQCFGVIVTADQEPKDYYMIASTRFLKKPLTTTGLLRYEGG 303

Query: 303 NSPASPEIPEAPIGWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKLLNSAT 362
             PAS ++P AP+GWAWSLNQ+R+FRWNLTASAARPNPQGSYHYG INITRTIKL+N+  
Sbjct: 304 KGPASSQLPAAPVGWAWSLNQYRSFRWNLTASAARPNPQGSYHYGKINITRTIKLVNTQG 363

Query: 363 LVDGKLRYAINDVSHVDPETPLKLAQYFNVADNVFKYDMISDEGSVEG-RDVTIAPNVVN 422
            VDGKLRYA++ VSH DPETPLKLA+YF VAD VFKYD ISD  + +  +++ I PNV+N
Sbjct: 364 KVDGKLRYALSGVSHTDPETPLKLAEYFGVADKVFKYDTISDNPNPDQIKNIKIEPNVLN 423

Query: 423 ATYRNFVEIIFENHEKSMQSWHLDGYSFFAVAIEPGRWAPEKRKNYNLLDAVHRHTIQVF 482
            T+R F+E++FENHE+S+QSWHLDGYSFFAVA+EPG W PEKRKNYNLLDAV RHT+QV+
Sbjct: 424 ITHRTFIEVVFENHERSVQSWHLDGYSFFAVAVEPGTWTPEKRKNYNLLDAVSRHTVQVY 483

Query: 483 PKSWAAVLVTLDNAGMWNLRSELTENRYLGQQLYFSVLSPANSLRDEYNIPDNTLLCGLV 542
           PK WAA+L+T DN GMWN+RSE  E RYLGQQLY SVLSP  SLRDEYN+P+ +L CGLV
Sbjct: 484 PKCWAAILLTFDNCGMWNIRSENAERRYLGQQLYASVLSPEKSLRDEYNMPETSLQCGLV 543

Query: 543 KDMPLPKPY 551
           K  P   PY
Sbjct: 544 KGKPKVNPY 552

BLAST of CmaCh06G009150 vs. TAIR 10
Match: AT3G13400.1 (SKU5 similar 13 )

HSP 1 Score: 864.4 bits (2232), Expect = 5.0e-251
Identity = 396/552 (71.74%), Postives = 461/552 (83.51%), Query Frame = 0

Query: 1   MAGLTFTMLRICLLAVALSTVRGEDPYFFFTWNVTYGTISPLGVPQQGILINGQFPGPNI 60
           M G     + +C LA  ++ V   DPYF++TWNVTYGT +PLG+PQQ ILINGQFPGPN+
Sbjct: 1   MQGGRLLTVLVC-LASTVALVSAGDPYFYYTWNVTYGTAAPLGIPQQVILINGQFPGPNL 60

Query: 61  NSTSNNNIVVNVFNNLDEPFLLHWSGVQHRKNSWQDGLLGTNCPIPPGSNFTYHFQVKDQ 120
           NSTSNNN+V+NVFNNLDEPFLL WSG+QHRKNSWQDG+ GT+CPIP G+NFTYHFQ KDQ
Sbjct: 61  NSTSNNNVVINVFNNLDEPFLLTWSGLQHRKNSWQDGVTGTSCPIPAGTNFTYHFQPKDQ 120

Query: 121 IGSFFYYPSTAMHRAGGAFGGLRINSRLLIPVPYADPEDDYTVLAGDWYTKTHSTLKKLL 180
           IGS+FYYPSTA+HR  G FGGLR+NSRLLIPVPYADPEDD T+L  DWY K+H+ LK  L
Sbjct: 121 IGSYFYYPSTALHRFAGGFGGLRVNSRLLIPVPYADPEDDRTILINDWYAKSHTALKNFL 180

Query: 181 DSGRSIARPDGVLINGKTAKGDGTDEPLFTMKPGKTYKYRICNVGLKTSLNFRIQGHTMK 240
           DSGR++  PDGVLINGK+ K  G + PLFTMKPGKTYKYRICNVG K++LNFRIQGH MK
Sbjct: 181 DSGRTLGSPDGVLINGKSGKLGGNNAPLFTMKPGKTYKYRICNVGFKSTLNFRIQGHKMK 240

Query: 241 LVEMEGSHTVQNVYQSLDVHVGQCFSVLVTADQEPKDYYMVASTRFIKNILVGKAIVRYT 300
           LVEMEGSH +QN Y SLDVHVGQCF+VLVTADQ  K+YYMVASTRF+K  +    ++ Y 
Sbjct: 241 LVEMEGSHVLQNDYDSLDVHVGQCFAVLVTADQVAKNYYMVASTRFLKKEVSTVGVMSYE 300

Query: 301 NGNSPASPEIPEAPIGWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKLLNS 360
             N  AS +IP+AP+GWAWSLNQFR+FRWNLTASAARPNPQGSYHYG INITRTIKL N+
Sbjct: 301 GSNVQASSDIPKAPVGWAWSLNQFRSFRWNLTASAARPNPQGSYHYGKINITRTIKLANT 360

Query: 361 ATLVDGKLRYAINDVSHVDPETPLKLAQYFNVADNVFKYDMISDEGSVEGRDVTIAPNVV 420
             LV+GK+R+  N VSHVD ETPLKLA+YF +++ VFKY++I DE + +   +T+ PNV+
Sbjct: 361 KNLVNGKVRFGFNGVSHVDTETPLKLAEYFGMSEKVFKYNVIKDEPAAKITTLTVEPNVL 420

Query: 421 NATYRNFVEIIFENHEKSMQSWHLDGYSFFAVAIEPGRWAPEKRKNYNLLDAVHRHTIQV 480
           N T+R FVE++FENHEKSMQS+HLDGYSFFAVA EPGRW PEKR NYNLLDAV RHT+QV
Sbjct: 421 NITFRTFVEVVFENHEKSMQSFHLDGYSFFAVASEPGRWTPEKRNNYNLLDAVSRHTVQV 480

Query: 481 FPKSWAAVLVTLDNAGMWNLRSELTENRYLGQQLYFSVLSPANSLRDEYNIPDNTLLCGL 540
           +PKSW+A+L+T DNAGMWN+RSE  E RYLGQQLY SVLSP  SLRDEYNIP NT LCG+
Sbjct: 481 YPKSWSAILLTFDNAGMWNIRSENWERRYLGQQLYVSVLSPEKSLRDEYNIPLNTNLCGI 540

Query: 541 VKDMPLPKPYTI 553
           VK +PLP PYTI
Sbjct: 541 VKGLPLPTPYTI 551

BLAST of CmaCh06G009150 vs. TAIR 10
Match: AT1G55560.1 (SKU5 similar 14 )

HSP 1 Score: 858.2 bits (2216), Expect = 3.6e-249
Identity = 393/551 (71.32%), Postives = 464/551 (84.21%), Query Frame = 0

Query: 1   MAGLTFTMLRICLLAVALSTVRGEDPYFFFTWNVTYGTISPLGVPQQGILINGQFPGPNI 60
           M G   T+L +CL++  ++ V   DPYFF TWNVTYGT SPLGVPQ+ ILINGQFPGPN+
Sbjct: 1   MEGRLLTVL-VCLVS-TVAIVNAGDPYFFHTWNVTYGTASPLGVPQKVILINGQFPGPNL 60

Query: 61  NSTSNNNIVVNVFNNLDEPFLLHWSGVQHRKNSWQDGLLGTNCPIPPGSNFTYHFQVKDQ 120
           NSTSNNN+V+NVFN+LDEPFLL WSG+QHRKN WQDG+ GT+CPIP G NFTYHFQ KDQ
Sbjct: 61  NSTSNNNVVINVFNHLDEPFLLTWSGIQHRKNCWQDGVAGTSCPIPAGQNFTYHFQPKDQ 120

Query: 121 IGSFFYYPSTAMHRAGGAFGGLRINSRLLIPVPYADPEDDYTVLAGDWYTKTHSTLKKLL 180
           IGS+FYYP+T++HR  G FGGLR+NSRLLIPVPYADPEDDYTVL GDWYT  H+ LK  L
Sbjct: 121 IGSYFYYPTTSLHRFAGGFGGLRVNSRLLIPVPYADPEDDYTVLLGDWYTAGHTALKNFL 180

Query: 181 DSGRSIARPDGVLINGKTAKGDGTDEPLFTMKPGKTYKYRICNVGLKTSLNFRIQGHTMK 240
           DSGR++  P+GVLINGK+ K  G +EPLFTMKPGKTYKYR+CNVG K++LNFRIQ H MK
Sbjct: 181 DSGRTLGLPNGVLINGKSGKVGGKNEPLFTMKPGKTYKYRLCNVGFKSTLNFRIQNHKMK 240

Query: 241 LVEMEGSHTVQNVYQSLDVHVGQCFSVLVTADQEPKDYYMVASTRFIKNILVGKAIVRYT 300
           LVEMEGSH +QN Y SLDVHVGQCFSVLVTA+Q  KDYYMVASTRF+K  L    ++RY 
Sbjct: 241 LVEMEGSHVIQNDYDSLDVHVGQCFSVLVTANQAAKDYYMVASTRFLKKELSTVGVIRYE 300

Query: 301 NGNSPASPEIPEAPIGWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKLLNS 360
             N  AS E+P+AP+GWAWSLNQFR+FRWNLT++AARPNPQGSYHYG INITR+IKL+NS
Sbjct: 301 GSNVQASTELPKAPVGWAWSLNQFRSFRWNLTSNAARPNPQGSYHYGKINITRSIKLVNS 360

Query: 361 ATLVDGKLRYAINDVSHVDPETPLKLAQYFNVADNVFKYDMISDEGSVEGRDVTIAPNVV 420
            ++VDGK+R+  N VSHVD ETPLKLA+YF +++ VFKY++I DE + +   +T+ PNV+
Sbjct: 361 KSVVDGKVRFGFNGVSHVDTETPLKLAEYFQMSEKVFKYNVIKDEPAAKITALTVQPNVL 420

Query: 421 NATYRNFVEIIFENHEKSMQSWHLDGYSFFAVAIEPGRWAPEKRKNYNLLDAVHRHTIQV 480
           N T+R FVEIIFENHEK+MQS+HLDGYSFFAVA EPGRW PEKR+NYNLLDAV RHT+QV
Sbjct: 421 NITFRTFVEIIFENHEKTMQSFHLDGYSFFAVASEPGRWTPEKRENYNLLDAVSRHTVQV 480

Query: 481 FPKSWAAVLVTLDNAGMWNLRSELTENRYLGQQLYFSVLSPANSLRDEYNIPDNTLLCGL 540
           +PKSW+A+L+T DNAGMWN+RSE  E +YLG+QLY SVLSP  SLRDEYNIP NT LCG+
Sbjct: 481 YPKSWSAILLTFDNAGMWNIRSENLERKYLGEQLYVSVLSPEKSLRDEYNIPLNTNLCGI 540

Query: 541 VKDMPLPKPYT 552
           VK +PLP  Y+
Sbjct: 541 VKGLPLPAHYS 549

BLAST of CmaCh06G009150 vs. TAIR 10
Match: AT1G21860.1 (SKU5 similar 7 )

HSP 1 Score: 643.3 bits (1658), Expect = 1.8e-184
Identity = 303/533 (56.85%), Postives = 382/533 (71.67%), Query Frame = 0

Query: 7   TMLRICLLAVALSTVRGEDPYFFFTWNVTYGTISPLGVPQQGILINGQFPGPNINSTSNN 66
           T   I  L   +S    EDPY FF W+VTYG ISPLGV QQGILING+FPGP+I S +N+
Sbjct: 8   TRAMITTLLFLISLAFAEDPYRFFEWHVTYGNISPLGVAQQGILINGKFPGPDIISITND 67

Query: 67  NIVVNVFNNLDEPFLLHWSGVQHRKNSWQDGLLGTNCPIPPGSNFTYHFQVKDQIGSFFY 126
           N+++NVFN+LDEPFLL W+G+++ KNS+QDG+ GT CPIPPG N+TY  QVKDQIGSF+Y
Sbjct: 68  NLIINVFNHLDEPFLLSWNGIRNWKNSFQDGVYGTMCPIPPGKNYTYALQVKDQIGSFYY 127

Query: 127 YPSTAMHRAGGAFGGLRINSRLLIPVPYADPEDDYTVLAGDWYTKTHSTLKKLLDSGRSI 186
           +PS   H+A G FGG+RI+SR LIPVP+  P DDYT+L GDWY   H  LK  LD+G  +
Sbjct: 128 FPSLGFHKAAGGFGGIRISSRALIPVPFPTPADDYTLLVGDWYKTNHKDLKAQLDNGGKL 187

Query: 187 ARPDGVLINGKTAKGDGTDEPLFTMKPGKTYKYRICNVGLKTSLNFRIQGHTMKLVEMEG 246
             PDG+LING+++           ++PGKTY+ RI NVGL+ SLNFRIQ HTMKLVE+EG
Sbjct: 188 PLPDGILINGRSSGA------TLNIEPGKTYRLRISNVGLQNSLNFRIQNHTMKLVEVEG 247

Query: 247 SHTVQNVYQSLDVHVGQCFSVLVTADQEPKDYYMVASTRFIKNILVGKAIVRYTNGNSPA 306
            +T+QN++ SLDVHVGQ +SVL+TADQ  KDYY+V S+RF   IL    ++ Y+N  +P 
Sbjct: 248 RYTIQNLFSSLDVHVGQSYSVLITADQPAKDYYVVVSSRFTSKILTTTGVLHYSNSVAPV 307

Query: 307 SPEIPEAPIGWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKLLNSATLVDG 366
           S  IP+ PI  +WS NQ R  R NLTAS  RPNPQGSY YG INITRTI+L N+   ++G
Sbjct: 308 SGPIPDGPIKLSWSFNQARAIRTNLTASGPRPNPQGSYRYGVINITRTIRLANNLGHIEG 367

Query: 367 KLRYAINDVSHVDPETPLKLAQYFNVADNVFKYDMISDEGSVEGRDVTIAPNVVNATYRN 426
           K RYA+N  S    +TPLKL  YF + D V+K   ISD+ +     +    +V+ A +R 
Sbjct: 368 KQRYAVNSASFYPADTPLKLVDYFKI-DGVYKPGSISDQPT--NGAIFPTTSVMQADFRA 427

Query: 427 FVEIIFENHEKSMQSWHLDGYSFFAVAIEPGRWAPEKRKNYNLLDAVHRHTIQVFPKSWA 486
           FVE+IFEN E  +QSWHLDGYSF+ V +E G+W+P  RK YNL DA+ R TIQV+P+SW 
Sbjct: 428 FVEVIFENSEDIVQSWHLDGYSFYVVGMELGKWSPASRKVYNLNDAILRCTIQVYPRSWT 487

Query: 487 AVLVTLDNAGMWNLRSELTENRYLGQQLYFSVLSPANSLRDEYNIPDNTLLCG 540
           A+ + LDN GMWN+RSE+ E +YLGQQ Y  V + + SLRDEY IP N LLCG
Sbjct: 488 AIYIALDNVGMWNMRSEIWERQYLGQQFYMRVYTTSTSLRDEYLIPKNALLCG 531

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q006242.0e-25271.38L-ascorbate oxidase homolog OS=Brassica napus OX=3708 GN=Bp10 PE=2 SV=1[more]
P291623.6e-23868.06L-ascorbate oxidase homolog OS=Nicotiana tabacum OX=4097 PE=2 SV=1[more]
Q9SU401.2e-15648.48Monocopper oxidase-like protein SKU5 OS=Arabidopsis thaliana OX=3702 GN=SKU5 PE=... [more]
Q8VXX51.5e-15147.40Monocopper oxidase-like protein SKS1 OS=Arabidopsis thaliana OX=3702 GN=SKS1 PE=... [more]
Q9FHN62.4e-14945.81Monocopper oxidase-like protein SKS2 OS=Arabidopsis thaliana OX=3702 GN=SKS2 PE=... [more]
Match NameE-valueIdentityDescription
A0A6J1IH900.0e+00100.00L-ascorbate oxidase homolog OS=Cucurbita maxima OX=3661 GN=LOC111472953 PE=3 SV=... [more]
A0A6J1FTA80.0e+0098.55L-ascorbate oxidase homolog OS=Cucurbita moschata OX=3662 GN=LOC111447934 PE=3 S... [more]
A0A5A7T1B73.0e-30489.51L-ascorbate oxidase-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27... [more]
A0A5D3BIA54.0e-30489.33L-ascorbate oxidase-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676... [more]
A0A1S3AX964.0e-30489.33L-ascorbate oxidase homolog OS=Cucumis melo OX=3656 GN=LOC103483330 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_022974314.10.0e+00100.00L-ascorbate oxidase homolog [Cucurbita maxima][more]
XP_022943104.10.0e+0098.55L-ascorbate oxidase homolog [Cucurbita moschata][more]
KAG6596990.10.0e+0098.37L-ascorbate oxidase-like protein, partial [Cucurbita argyrosperma subsp. sororia... [more]
XP_023539597.10.0e+0097.64L-ascorbate oxidase homolog [Cucurbita pepo subsp. pepo][more]
KAA0036016.16.3e-30489.51L-ascorbate oxidase-like protein [Cucumis melo var. makuwa][more]
Match NameE-valueIdentityDescription
AT3G13390.11.0e-25672.23SKU5 similar 11 [more]
AT1G55570.11.4e-25672.50SKU5 similar 12 [more]
AT3G13400.15.0e-25171.74SKU5 similar 13 [more]
AT1G55560.13.6e-24971.32SKU5 similar 14 [more]
AT1G21860.11.8e-18456.85SKU5 similar 7 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001117Multicopper oxidase, type 1PFAMPF00394Cu-oxidasecoord: 159..289
e-value: 8.0E-41
score: 139.8
IPR011707Multicopper oxidase, N-termianlPFAMPF07732Cu-oxidase_3coord: 33..147
e-value: 1.5E-39
score: 134.7
IPR008972CupredoxinGENE3D2.60.40.420coord: 157..337
e-value: 2.5E-37
score: 130.9
IPR008972CupredoxinGENE3D2.60.40.420coord: 29..148
e-value: 1.9E-33
score: 117.3
IPR008972CupredoxinGENE3D2.60.40.420coord: 347..545
e-value: 2.8E-35
score: 123.9
IPR008972CupredoxinSUPERFAMILY49503Cupredoxinscoord: 24..173
IPR008972CupredoxinSUPERFAMILY49503Cupredoxinscoord: 349..538
IPR008972CupredoxinSUPERFAMILY49503Cupredoxinscoord: 156..338
IPR011706Multicopper oxidase, C-terminalPFAMPF07731Cu-oxidase_2coord: 382..520
e-value: 1.7E-25
score: 89.3
IPR045087Multicopper oxidasePANTHERPTHR11709MULTI-COPPER OXIDASEcoord: 7..543
NoneNo IPR availablePANTHERPTHR11709:SF246L-ASCORBATE OXIDASE-LIKE PROTEINcoord: 7..543
IPR034271Ascorbate oxidase homologue, second cupredoxin domainCDDcd13872CuRO_2_AAO_like_1coord: 159..299
e-value: 1.94543E-86
score: 261.954

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh06G009150.1CmaCh06G009150.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0005507 copper ion binding
molecular_function GO:0016491 oxidoreductase activity