CmaCh06G008720 (gene) Cucurbita maxima (Rimu) v1.1

Overview


Name	CmaCh06G008720
Type	gene
Organism	Cucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Description	(1->3)-beta-glucan endohydrolase
Location	Cma_Chr06: 5086398 .. 5088174 (+)
RNA-Seq Expression	CmaCh06G008720
Synteny	CmaCh06G008720

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TGTTGCAAGTAGCTCATCAGCCAACAAAAACTGTAACCAGTGAAATGCCAGGAATGGAGACAAGTAAACGGCTCCAAGAAATTCAGACCAATCAATAAGGCGGTGAGGATCGGTGTACACAATTTCTCTTCTTCTCTATATAATCACAGTTTCGGGGGAAACCCATTTCAAACCTCTATTGATCATGGCCATGCCCTTCTTCCCTTTCCTCTATCTCCTCTTTTCCGCCACCGCTCACGGAGAACCCGGATTGATTGGGGTTAACTACGGCCGAATCGCCGACAACCTTCCCCCGCCCGACAAAGTTGTTCATCTCCTCAAACAGCAAGGGATCGACCGTATCAAAGTCTATGACACCGATGCCGCCGTCCTTACTGCCCTTTCCAACTCCAACATCTCCATCGTTGTTGCCCTTCCCAACCACCTCCTCTCCTCCGCCGCAGCGGATTCTTCCTTCACCGACAATTGGGTCCAATCCAAAATCTCTAATTATTACCCCGCCACCAAAATCAACGCCGTCGCCGTCGGCAACGAGGTCTTCGTCGACCCCAACAACACCACCAATTTCCTCATCCCCGCCATGAAAAACGTCTACTCCTCGCTACAAAAGTATAACCTCCACGACTCCATCAAAGTCTCCTCGCCCATTGCCCTCACCGCCCTCGCTTCCTCCTACCCGACCTCCTCCGGTTCCTTTAAACCAGACCTTATCGAGCCAGTCATCAAACCCATGCTCGACTTTCTCCACCAGACCGAATCTTACCTCATGGTTAACGCCTACCCCTTCTTCGCATACGCCGCTAACTCCGACCAGATTTCTCTGGACTACGCTCTCTTCCGTGAGAACTCCGGCGTTGTGGACCCCGGCAATGGGTTGAAATATTTCAGTCTTTTCGAGGCTCAAGTGGACGCTGTTTACGCCGCCATGATGGCGCTTAAATACGACGACATTAAAGTGGTGGTTACGGAGACTGGGTGGCCGTCGATAGGGGAGGAGAAGGAAGTGGGAGCGAGTAAGGAGAATGCGGCGTCGTACAACGGCAATTTAGTGCGGAGAGTTCTGACAGGGAGAGGGACCCCCTTGAGACCAGAAGACCCATTGGACGTCTTTCTGTTCGCGTTGTTCAATGAGAATCAGAAACCGGGGCCCACCTCTGAGAGAAATTACGGTTTGTTTTACCCCAACGAGGAGAAGGTTTATGAGATTCCTTTCACACTCGCGGACATGGCGAGTGAGACTCCGACGCCGGTGAACGGGAGTAAGGCTCAGGTGCCGCCGTCCAAAGGGGATACCTGGTGTGTGGCTAATGGGAACGCGGCGGCGGAGAAGCTGCGGGAGGGTTTGGATTATGCCTGTGGAGAGGGGGGAGCTGATTGTCGTGCGATTCAGCCAGGTTCCACGTGTTACAATCCTAACTCACTTGAGGCACACGCTTCGTACGCGTTCAATAGCTATTACCAGAAGATGGGTCGTGCGAGTGGCACGTGCTACTTTGGTGGTGTTGCCTACGTCGTCACTCAACCTCCCCGTAAGTACCAAATTGGAAGTTCAATAAATAATACCGTATTATATATTTAATCTAACAAACGGTGTCGTTTTGTGGTTGCAGAGTACGGAAACTGCGAGTTCCCTACAGGGTACTGAGCTGGGGCAGGCCGCTATAGAAATTTATAGAAACTAATAAAAGAAAACACAGCATTTTAATTTCATTTAGTAATATATTATTATTTGTATGTAACAGTGAAATATTCAGTTATAAAAATTATTGTTTCCAGA

mRNA sequence

TGTTGCAAGTAGCTCATCAGCCAACAAAAACTGTAACCAGTGAAATGCCAGGAATGGAGACAAGTAAACGGCTCCAAGAAATTCAGACCAATCAATAAGGCGGTGAGGATCGGTGTACACAATTTCTCTTCTTCTCTATATAATCACAGTTTCGGGGGAAACCCATTTCAAACCTCTATTGATCATGGCCATGCCCTTCTTCCCTTTCCTCTATCTCCTCTTTTCCGCCACCGCTCACGGAGAACCCGGATTGATTGGGGTTAACTACGGCCGAATCGCCGACAACCTTCCCCCGCCCGACAAAGTTGTTCATCTCCTCAAACAGCAAGGGATCGACCGTATCAAAGTCTATGACACCGATGCCGCCGTCCTTACTGCCCTTTCCAACTCCAACATCTCCATCGTTGTTGCCCTTCCCAACCACCTCCTCTCCTCCGCCGCAGCGGATTCTTCCTTCACCGACAATTGGGTCCAATCCAAAATCTCTAATTATTACCCCGCCACCAAAATCAACGCCGTCGCCGTCGGCAACGAGGTCTTCGTCGACCCCAACAACACCACCAATTTCCTCATCCCCGCCATGAAAAACGTCTACTCCTCGCTACAAAAGTATAACCTCCACGACTCCATCAAAGTCTCCTCGCCCATTGCCCTCACCGCCCTCGCTTCCTCCTACCCGACCTCCTCCGGTTCCTTTAAACCAGACCTTATCGAGCCAGTCATCAAACCCATGCTCGACTTTCTCCACCAGACCGAATCTTACCTCATGGTTAACGCCTACCCCTTCTTCGCATACGCCGCTAACTCCGACCAGATTTCTCTGGACTACGCTCTCTTCCGTGAGAACTCCGGCGTTGTGGACCCCGGCAATGGGTTGAAATATTTCAGTCTTTTCGAGGCTCAAGTGGACGCTGTTTACGCCGCCATGATGGCGCTTAAATACGACGACATTAAAGTGGTGGTTACGGAGACTGGGTGGCCGTCGATAGGGGAGGAGAAGGAAGTGGGAGCGAGTAAGGAGAATGCGGCGTCGTACAACGGCAATTTAGTGCGGAGAGTTCTGACAGGGAGAGGGACCCCCTTGAGACCAGAAGACCCATTGGACGTCTTTCTGTTCGCGTTGTTCAATGAGAATCAGAAACCGGGGCCCACCTCTGAGAGAAATTACGGTTTGTTTTACCCCAACGAGGAGAAGGTTTATGAGATTCCTTTCACACTCGCGGACATGGCGAGTGAGACTCCGACGCCGGTGAACGGGAGTAAGGCTCAGGTGCCGCCGTCCAAAGGGGATACCTGGTGTGTGGCTAATGGGAACGCGGCGGCGGAGAAGCTGCGGGAGGGTTTGGATTATGCCTGTGGAGAGGGGGGAGCTGATTGTCGTGCGATTCAGCCAGGTTCCACGTGTTACAATCCTAACTCACTTGAGGCACACGCTTCGTACGCGTTCAATAGCTATTACCAGAAGATGGGTCGTGCGAGTGGCACGTGCTACTTTGGTGGTGTTGCCTACGTCGTCACTCAACCTCCCCAGTACGGAAACTGCGAGTTCCCTACAGGGTACTGAGCTGGGGCAGGCCGCTATAGAAATTTATAGAAACTAATAAAAGAAAACACAGCATTTTAATTTCATTTAGTAATATATTATTATTTGTATGTAACAGTGAAATATTCAGTTATAAAAATTATTGTTTCCAGA

Coding sequence (CDS)

ATGGCCATGCCCTTCTTCCCTTTCCTCTATCTCCTCTTTTCCGCCACCGCTCACGGAGAACCCGGATTGATTGGGGTTAACTACGGCCGAATCGCCGACAACCTTCCCCCGCCCGACAAAGTTGTTCATCTCCTCAAACAGCAAGGGATCGACCGTATCAAAGTCTATGACACCGATGCCGCCGTCCTTACTGCCCTTTCCAACTCCAACATCTCCATCGTTGTTGCCCTTCCCAACCACCTCCTCTCCTCCGCCGCAGCGGATTCTTCCTTCACCGACAATTGGGTCCAATCCAAAATCTCTAATTATTACCCCGCCACCAAAATCAACGCCGTCGCCGTCGGCAACGAGGTCTTCGTCGACCCCAACAACACCACCAATTTCCTCATCCCCGCCATGAAAAACGTCTACTCCTCGCTACAAAAGTATAACCTCCACGACTCCATCAAAGTCTCCTCGCCCATTGCCCTCACCGCCCTCGCTTCCTCCTACCCGACCTCCTCCGGTTCCTTTAAACCAGACCTTATCGAGCCAGTCATCAAACCCATGCTCGACTTTCTCCACCAGACCGAATCTTACCTCATGGTTAACGCCTACCCCTTCTTCGCATACGCCGCTAACTCCGACCAGATTTCTCTGGACTACGCTCTCTTCCGTGAGAACTCCGGCGTTGTGGACCCCGGCAATGGGTTGAAATATTTCAGTCTTTTCGAGGCTCAAGTGGACGCTGTTTACGCCGCCATGATGGCGCTTAAATACGACGACATTAAAGTGGTGGTTACGGAGACTGGGTGGCCGTCGATAGGGGAGGAGAAGGAAGTGGGAGCGAGTAAGGAGAATGCGGCGTCGTACAACGGCAATTTAGTGCGGAGAGTTCTGACAGGGAGAGGGACCCCCTTGAGACCAGAAGACCCATTGGACGTCTTTCTGTTCGCGTTGTTCAATGAGAATCAGAAACCGGGGCCCACCTCTGAGAGAAATTACGGTTTGTTTTACCCCAACGAGGAGAAGGTTTATGAGATTCCTTTCACACTCGCGGACATGGCGAGTGAGACTCCGACGCCGGTGAACGGGAGTAAGGCTCAGGTGCCGCCGTCCAAAGGGGATACCTGGTGTGTGGCTAATGGGAACGCGGCGGCGGAGAAGCTGCGGGAGGGTTTGGATTATGCCTGTGGAGAGGGGGGAGCTGATTGTCGTGCGATTCAGCCAGGTTCCACGTGTTACAATCCTAACTCACTTGAGGCACACGCTTCGTACGCGTTCAATAGCTATTACCAGAAGATGGGTCGTGCGAGTGGCACGTGCTACTTTGGTGGTGTTGCCTACGTCGTCACTCAACCTCCCCAGTACGGAAACTGCGAGTTCCCTACAGGGTACTGA

Protein sequence

MAMPFFPFLYLLFSATAHGEPGLIGVNYGRIADNLPPPDKVVHLLKQQGIDRIKVYDTDAAVLTALSNSNISIVVALPNHLLSSAAADSSFTDNWVQSKISNYYPATKINAVAVGNEVFVDPNNTTNFLIPAMKNVYSSLQKYNLHDSIKVSSPIALTALASSYPTSSGSFKPDLIEPVIKPMLDFLHQTESYLMVNAYPFFAYAANSDQISLDYALFRENSGVVDPGNGLKYFSLFEAQVDAVYAAMMALKYDDIKVVVTETGWPSIGEEKEVGASKENAASYNGNLVRRVLTGRGTPLRPEDPLDVFLFALFNENQKPGPTSERNYGLFYPNEEKVYEIPFTLADMASETPTPVNGSKAQVPPSKGDTWCVANGNAAAEKLREGLDYACGEGGADCRAIQPGSTCYNPNSLEAHASYAFNSYYQKMGRASGTCYFGGVAYVVTQPPQYGNCEFPTGY

Homology

[Close]

BLAST of CmaCh06G008720 vs. ExPASy Swiss-Prot
Match: Q8VYE5 (Glucan endo-1,3-beta-glucosidase 12 OS=Arabidopsis thaliana OX=3702 GN=At4g29360 PE=2 SV=1)

HSP 1 Score: 379.0 bits (972), Expect = 7.5e-104
Identity = 191/453 (42.16%), Postives = 284/453 (62.69%), Query Frame = 0


Query: 19  GEPGLIGVNYGRIADNLPPPDKVVHLLKQQGIDRIKVYDTDAAVLTALSNSNISIVVALP 78
           G    IG+ YGR ADNLP P++V  L++   I  +++YD +  VL A +N+ I +++ +P
Sbjct: 22  GMASKIGICYGRNADNLPSPNRVSELIQHLNIKFVRIYDANIDVLKAFANTGIELMIGVP 81

Query: 79  NHLLSSAAADSSFTDNWVQSKISNYYPATKINAVAVGNEVFVDPNNTTNFLIPAMKNVYS 138
           N  L + A   S  D W+ + I  YYP+TKI +++VG EV   P+N T  ++PAM+N+++
Sbjct: 82  NADLLAFAQFQSNVDTWLSNNILPYYPSTKITSISVGLEVTEAPDNATGLVLPAMRNIHT 141

Query: 139 SLQKYNLHDSIKVSSPIALTALASSYPTSSGSFKPDLIEPVIKPMLDFLHQTESYLMVNA 198
           +L+K  L   IK+SS  +L  L+ S+P SS SF        +KPML+FL + ES  M++ 
Sbjct: 142 ALKKSGLDKKIKISSSHSLAILSRSFPPSSASFSKK-HSAFLKPMLEFLVENESPFMIDL 201

Query: 199 YPFFAYAANSDQISLDYALFRENSGVVDPGNGLKYFSLFEAQVDAVYAAMMALKYDDIKV 258
           YP++AY  +++++ L+YALF  +S VVDP  GL Y ++F+AQ+DA+Y A+ A+ +  +KV
Sbjct: 202 YPYYAYRDSTEKVPLEYALFESSSQVVDPATGLLYSNMFDAQLDAIYFALTAMSFKTVKV 261

Query: 259 VVTETGWPSIGEEKEVGASKENAASYNGNLVRRVLTGRGTPLRPEDPLDVFLFALFNENQ 318
           +VTE+GWPS G  KE  A+ ENA +YN NL+R V+   GTP +P + +DV+LF+LFNEN+
Sbjct: 262 MVTESGWPSKGSPKETAATPENALAYNTNLIRHVIGDPGTPAKPGEEIDVYLFSLFNENR 321

Query: 319 KPGPTSERNYGLFYPNEEKVYEIPFTLADMASETPTPVNGSKAQVPPSK----------- 378
           KPG  SERN+G+FY N   VY + FT  +    +PT      +  P S            
Sbjct: 322 KPGIESERNWGMFYANGTNVYALDFTGENTTPVSPTNSTTGTSPSPSSSPIINGNSTVTI 381

Query: 379 -----GDT--WCVANGNAAAEKLREGLDYACGEGGADCRAIQPGSTCYNPNSLEAHASYA 438
                G T  WC+A+  A+  +L+  LD+ACG G  DC A+QP   C+ P+++ +HASYA
Sbjct: 382 GGGGGGGTKKWCIASSQASVTELQTALDWACGPGNVDCSAVQPDQPCFEPDTVLSHASYA 441

Query: 439 FNSYYQKMGRASGTCYFGGVAYVVTQPPQYGNC 454
           FN+YYQ+ G +S  C F G +  V + P YGNC
Sbjct: 442 FNTYYQQSGASSIDCSFNGASVEVDKDPSYGNC 473

[Close]

BLAST of CmaCh06G008720 vs. ExPASy Swiss-Prot
Match: Q9FJU9 (Glucan endo-1,3-beta-glucosidase 13 OS=Arabidopsis thaliana OX=3702 GN=At5g56590 PE=2 SV=1)

HSP 1 Score: 370.9 bits (951), Expect = 2.0e-101
Identity = 182/438 (41.55%), Postives = 281/438 (64.16%), Query Frame = 0


Query: 22  GLIGVNYGRIADNLPPPDKVVHLLKQQGIDRIKVYDTDAAVLTALSNSNISIVVALPNHL 81
           G +GV YGR AD+LP P KVV L++Q  I  +++YD ++ VL A  N++I +++ +PN  
Sbjct: 24  GKVGVCYGRSADDLPTPSKVVQLIQQHNIKYVRIYDYNSQVLKAFGNTSIELMIGVPNSD 83

Query: 82  LSSAAADSSFTDNWVQSKISNYYPATKINAVAVGNEVFVDPN-NTTNFLIPAMKNVYSSL 141
           L++ +   S  D W+++ +  YYP TKI  + VG E   DP+ N ++F++PAM+NV ++L
Sbjct: 84  LNAFSQSQSNVDTWLKNSVLPYYPTTKITYITVGAESTDDPHINASSFVVPAMQNVLTAL 143

Query: 142 QKYNLHDSIKVSSPIALTALASSYPTSSGSFKPDLIEPVIKPMLDFLHQTESYLMVNAYP 201
           +K  L   IKVS+ ++L  L+ S+P S+G+F        ++PML+FL + +S  M++ YP
Sbjct: 144 RKVGLSRRIKVSTTLSLGILSRSFPPSAGAFNSSYAY-FLRPMLEFLAENKSPFMIDLYP 203

Query: 202 FFAYAANSDQISLDYALFRENSGVVDPGNGLKYFSLFEAQVDAVYAAMMALKYDDIKVVV 261
           ++AY  + + +SLDY LF  +S V+DP  GL Y ++F+AQVDA+Y A+ AL +  IK++V
Sbjct: 204 YYAYRDSPNNVSLDYVLFESSSEVIDPNTGLLYKNMFDAQVDALYYALTALNFRTIKIMV 263

Query: 262 TETGWPSIGEEKE-VGASKENAASYNGNLVRRVLTGRGTPLRPEDPLDVFLFALFNENQK 321
           TETGWP+ G  KE   AS +NA +YN N++R V+T +GTP +P + ++V++F+LFNEN+K
Sbjct: 264 TETGWPTKGSPKEKAAASSDNAETYNSNIIRHVVTNQGTPAKPGEAMNVYIFSLFNENRK 323

Query: 322 PGPTSERNYGLFYPNEEKVYEIPFTLADMASETPTPVNGSKAQVPPSKGDTWCVANGNAA 381
            G  SERN+GLFYP++  VY++ FT       + +    S         ++WC+A+  A+
Sbjct: 324 AGLDSERNWGLFYPDQTSVYQLDFTGKSNGFHSNSSGTNSS-----GSSNSWCIASSKAS 383

Query: 382 AEKLREGLDYACGEGGADCRAIQPGSTCYNPNSLEAHASYAFNSYYQKMGRASGTCYFGG 441
              L+  LD+ACG G  DC AIQP   C+ P++L +HAS+ FNSY+Q+       C FGG
Sbjct: 384 ERDLKGALDWACGPGNVDCTAIQPSQPCFQPDTLVSHASFVFNSYFQQNRATDVACSFGG 443

Query: 442 VAYVVTQPPQYGNCEFPT 458
               V + P Y  C + T
Sbjct: 444 AGVKVNKDPSYDKCIYIT 455

[Close]

BLAST of CmaCh06G008720 vs. ExPASy Swiss-Prot
Match: Q9M069 (Glucan endo-1,3-beta-glucosidase 7 OS=Arabidopsis thaliana OX=3702 GN=At4g34480 PE=1 SV=2)

HSP 1 Score: 362.5 bits (929), Expect = 7.2e-99
Identity = 190/456 (41.67%), Postives = 282/456 (61.84%), Query Frame = 0


Query: 5   FFPFLYLLFSATAHGEPGLIGVNYGRIADNLPPPDKVVHLLKQQGIDRIKVYDTDAAVLT 64
           +F  ++L    ++H EP  IGVNYG++ADNLPPP + V LL+   I ++++Y  D A++ 
Sbjct: 8   YFLLIFLSHFPSSHAEP-FIGVNYGQVADNLPPPSETVKLLQSTSIQKVRLYGADPAIIK 67

Query: 65  ALSNSNISIVVALPNHLLSSAAADSSFTDNWVQSKISNYYPATKINAVAVGNEVFV--DP 124
           AL+ + + IV+   N  + S A+D +    W+ S +  +YPA+KI  + VGNE+ +  DP
Sbjct: 68  ALAGTGVGIVIGAANGDVPSLASDPNAATQWINSNVLPFYPASKIMLITVGNEILMSNDP 127

Query: 125 NNTTNFLIPAMKNVYSSLQKYNLHDSIKVSSPIALTALASSYPTSSGSFKPDLIEPVIKP 184
            N  N L+PAM+NV  +L+  +L   IKVS+  ++T L SS P SSGSF     +  +K 
Sbjct: 128 -NLVNQLLPAMQNVQKALEAVSLGGKIKVSTVNSMTVLGSSDPPSSGSFAAG-YQTGLKG 187

Query: 185 MLDFLHQTESYLMVNAYPFFAYAANSDQISLDYALFRENSGVVDPGNGLKYFSLFEAQVD 244
           +L FL  T S   +N YPFFAY ++    +L + LF  N+G VD   G+KY ++F+AQVD
Sbjct: 188 ILQFLSDTGSPFAINPYPFFAYQSDPRPETLAFCLFEPNAGRVDSKTGIKYTNMFDAQVD 247

Query: 245 AVYAAMMALKYDDIKVVVTETGWPSIGEEKEVGASKENAASYNGNLVRRVLTGRGTPLRP 304
           AV++A+ ++ ++ +++VV ETGW S G+  EVGAS +NA +YNGNL+  + +  GTPL P
Sbjct: 248 AVHSALKSMGFEKVEIVVAETGWASRGDANEVGASVDNAKAYNGNLIAHLRSMVGTPLMP 307

Query: 305 EDPLDVFLFALFNENQKPGPTSERNYGLFYPNEEKVYEIPFTLADMASETPTPVNGSKAQ 364
             P+D ++FAL++EN KPGP+SER +GLF  +   VY++    +  +S+TP+    S   
Sbjct: 308 GKPVDTYIFALYDENLKPGPSSERAFGLFKTDLSMVYDVGLAKSSSSSQTPSGKVTSSG- 367

Query: 365 VPPSKGDTWCVANGNAAAEKLREGLDYACGEGGADCRAIQPGSTCYNPNSLEAHASYAFN 424
                   WCV    A  E+L+  LD+ACG  G DC AIQPG  C+ PN++ +HA+YA N
Sbjct: 368 --------WCVPKKGATNEELQASLDWACGH-GIDCGAIQPGGACFEPNNVVSHAAYAMN 427

Query: 425 SYYQKMGRASGTCYFGGVAYVVTQPPQYGNCEFPTG 459
            Y+QK  +    C F   A V +Q P Y NC +P G
Sbjct: 428 MYFQKSPKQPTDCDFSKTATVTSQNPSYNNCVYPGG 450

[Close]

BLAST of CmaCh06G008720 vs. ExPASy Swiss-Prot
Match: Q9ZU91 (Glucan endo-1,3-beta-glucosidase 3 OS=Arabidopsis thaliana OX=3702 GN=At2g01630 PE=2 SV=2)

HSP 1 Score: 345.9 bits (886), Expect = 7.0e-94
Identity = 185/459 (40.31%), Postives = 268/459 (58.39%), Query Frame = 0


Query: 1   MAMPFFPFLYLLFSATAHGEPGLIGVNYGRIADNLPPPDKVVHLLKQQGIDRIKVYDTDA 60
           MA     FL+ LF+++A  +  LIGVN G    N+P P +VV LLK Q I+R+++YD D 
Sbjct: 1   MAALLLLFLF-LFASSALSQDSLIGVNIGTEVTNMPSPTQVVALLKSQNINRVRLYDADR 60

Query: 61  AVLTALSNSNISIVVALPNHLLSSAAADSSFTDNWVQSKISNYYPATKINAVAVGNEVFV 120
           ++L A +++ + +++++PN  L   +  ++   NWV   ++ YYPAT I  +AVG+EV  
Sbjct: 61  SMLLAFAHTGVQVIISVPNDQLLGISQSNATAANWVTRNVAAYYPATNITTIAVGSEVLT 120

Query: 121 DPNNTTNFLIPAMKNVYSSLQKYNLHDSIKVSSPIALTALASSYPTSSGSFKPDLIEPVI 180
              N  + L+ A+K + ++L   NL   IKVS+P + T +  S+P S   F     +PVI
Sbjct: 121 SLTNAASVLVSALKYIQAALVTANLDRQIKVSTPHSSTIILDSFPPSQAFFNKTW-DPVI 180

Query: 181 KPMLDFLHQTESYLMVNAYPFFAYAANSDQISLDYALF---RENSGVVDPGNGLKYFSLF 240
            P+L FL  T S L++N YP+F Y  ++  I LDYALF   + N   VD    L Y ++F
Sbjct: 181 VPLLKFLQSTGSPLLLNVYPYFDYVQSNGVIPLDYALFQPLQANKEAVDANTLLHYTNVF 240

Query: 241 EAQVDAVYAAMMALKYDDIKVVVTETGWPSIGEEKEVGASKENAASYNGNLVRRVLTGRG 300
           +A VDA Y AM  L + +I +VVTE+GWPS G   E  A+ ENA +YN NL++ V+   G
Sbjct: 241 DAIVDAAYFAMSYLNFTNIPIVVTESGWPSKGGPSEHDATVENANTYNSNLIQHVINKTG 300

Query: 301 TPLRPEDPLDVFLFALFNENQKPGPTSERNYGLFYPNEEKVYEIPFTLADMASETPTPVN 360
           TP  P   +  +++ L+NE+ +PGP SE+N+GLFY N   VY +    A       T   
Sbjct: 301 TPKHPGTAVTTYIYELYNEDTRPGPVSEKNWGLFYTNGTPVYTLRLAGAGAILANDT--- 360

Query: 361 GSKAQVPPSKGDTWCVANGNAAAEKLREGLDYACGEGGADCRAIQPGSTCYNPNSLEAHA 420
                       T+C+A      + L+  LD+ACG G  DC A+  G +CY P+ + AH+
Sbjct: 361 ---------TNQTFCIAKEKVDRKMLQAALDWACGPGKVDCSALMQGESCYEPDDVVAHS 420

Query: 421 SYAFNSYYQKMGRASGTCYFGGVAYVVTQPPQYGNCEFP 457
           +YAFN+YYQKMG+ASG+C F GVA V T  P  G C FP
Sbjct: 421 TYAFNAYYQKMGKASGSCDFKGVATVTTTDPSRGTCVFP 445

[Close]

BLAST of CmaCh06G008720 vs. ExPASy Swiss-Prot
Match: Q94CD8 (Glucan endo-1,3-beta-glucosidase 4 OS=Arabidopsis thaliana OX=3702 GN=At3g13560 PE=2 SV=1)

HSP 1 Score: 339.7 bits (870), Expect = 5.0e-92
Identity = 178/454 (39.21%), Postives = 262/454 (57.71%), Query Frame = 0


Query: 5   FFPFLYLLFSATAHGEPGLIGVNYGRIADNLPPPDKVVHLLKQQGIDRIKVYDTDAAVLT 64
           F   L LL S  A      IGVN G    N+PPP  +V LLK Q I  +++YD ++ +L 
Sbjct: 7   FAEALLLLLSILACSNAAFIGVNIGTDLTNMPPPSDIVTLLKSQQITHVRLYDANSHMLK 66

Query: 65  ALSNSNISIVVALPNHLLSSAAADSSFTDNWVQSKISNYYPATKINAVAVGNEVFVDPNN 124
           A +N++I ++V + N  +       S    WV   ++ Y P+T I A+AVG+EV     +
Sbjct: 67  AFANTSIEVMVGVTNEEILKIGRFPSAAAAWVNKNVAAYIPSTNITAIAVGSEVLTTIPH 126

Query: 125 TTNFLIPAMKNVYSSLQKYNLHDSIKVSSPIALTALASSYPTSSGSFKPDLIEPVIKPML 184
               L  A+ N++ +L   NL+  +KVSSP+++  +   +P S+ +F P      +  +L
Sbjct: 127 VAPILASALNNIHKALVASNLNFKVKVSSPMSMDIMPKPFPPSTSTFSPSW-NTTVYQLL 186

Query: 185 DFLHQTESYLMVNAYPFFAYAANSDQISLDYALFRENSGV---VDPGNGLKYFSLFEAQV 244
            FL  T S+ M+NAYP++ Y   +    LDYALF++ S V   VDP   L Y S+F+A V
Sbjct: 187 QFLKNTGSFFMLNAYPYYGYTTANGIFPLDYALFKQLSPVKQIVDPNTLLHYNSMFDAMV 246

Query: 245 DAVYAAMMALKYDDIKVVVTETGWPSIGEEKEVGASKENAASYNGNLVRRVLTGRGTPLR 304
           DA Y +M AL +  I VVVTETGWPS G   E  A+  NA ++N NL++RVL   G P +
Sbjct: 247 DAAYYSMEALNFSKIPVVVTETGWPSSGGSDEAAATVANAETFNTNLIKRVLNNSGPPSQ 306

Query: 305 PEDPLDVFLFALFNENQKPGPTSERNYGLFYPNEEKVYEIPFTLADMASETPTPVNGSKA 364
           P+ P++ +++ L+NE+++ GP SERN+G+ +PN   VY             P  ++G  +
Sbjct: 307 PDIPINTYIYELYNEDKRSGPVSERNWGILFPNGTSVY-------------PLSLSGGSS 366

Query: 365 QVPPSKGDTWCVANGNAAAEKLREGLDYACGEGGADCRAIQPGSTCYNPNSLEAHASYAF 424
               +    +CVA  +A  +KL +GL++ACG+G A+C AIQPG  CY PN +++HAS+AF
Sbjct: 367 SAALNGSSMFCVAKADADDDKLVDGLNWACGQGRANCAAIQPGQPCYLPNDVKSHASFAF 426

Query: 425 NSYYQKMGRASGTCYFGGVAYVVTQPPQYGNCEF 456
           N YYQKM  A GTC F G A   T+ P Y  C +
Sbjct: 427 NDYYQKMKSAGGTCDFDGTAITTTRDPSYRTCAY 446

[Close]

BLAST of CmaCh06G008720 vs. ExPASy TrEMBL
Match: A0A6J1KYS6 ((1->3)-beta-glucan endohydrolase OS=Cucurbita maxima OX=3661 GN=LOC111498785 PE=3 SV=1)

HSP 1 Score: 926.4 bits (2393), Expect = 4.6e-266
Identity = 459/459 (100.00%), Postives = 459/459 (100.00%), Query Frame = 0


Query: 1   MAMPFFPFLYLLFSATAHGEPGLIGVNYGRIADNLPPPDKVVHLLKQQGIDRIKVYDTDA 60
           MAMPFFPFLYLLFSATAHGEPGLIGVNYGRIADNLPPPDKVVHLLKQQGIDRIKVYDTDA
Sbjct: 1   MAMPFFPFLYLLFSATAHGEPGLIGVNYGRIADNLPPPDKVVHLLKQQGIDRIKVYDTDA 60

Query: 61  AVLTALSNSNISIVVALPNHLLSSAAADSSFTDNWVQSKISNYYPATKINAVAVGNEVFV 120
           AVLTALSNSNISIVVALPNHLLSSAAADSSFTDNWVQSKISNYYPATKINAVAVGNEVFV
Sbjct: 61  AVLTALSNSNISIVVALPNHLLSSAAADSSFTDNWVQSKISNYYPATKINAVAVGNEVFV 120

Query: 121 DPNNTTNFLIPAMKNVYSSLQKYNLHDSIKVSSPIALTALASSYPTSSGSFKPDLIEPVI 180
           DPNNTTNFLIPAMKNVYSSLQKYNLHDSIKVSSPIALTALASSYPTSSGSFKPDLIEPVI
Sbjct: 121 DPNNTTNFLIPAMKNVYSSLQKYNLHDSIKVSSPIALTALASSYPTSSGSFKPDLIEPVI 180

Query: 181 KPMLDFLHQTESYLMVNAYPFFAYAANSDQISLDYALFRENSGVVDPGNGLKYFSLFEAQ 240
           KPMLDFLHQTESYLMVNAYPFFAYAANSDQISLDYALFRENSGVVDPGNGLKYFSLFEAQ
Sbjct: 181 KPMLDFLHQTESYLMVNAYPFFAYAANSDQISLDYALFRENSGVVDPGNGLKYFSLFEAQ 240

Query: 241 VDAVYAAMMALKYDDIKVVVTETGWPSIGEEKEVGASKENAASYNGNLVRRVLTGRGTPL 300
           VDAVYAAMMALKYDDIKVVVTETGWPSIGEEKEVGASKENAASYNGNLVRRVLTGRGTPL
Sbjct: 241 VDAVYAAMMALKYDDIKVVVTETGWPSIGEEKEVGASKENAASYNGNLVRRVLTGRGTPL 300

Query: 301 RPEDPLDVFLFALFNENQKPGPTSERNYGLFYPNEEKVYEIPFTLADMASETPTPVNGSK 360
           RPEDPLDVFLFALFNENQKPGPTSERNYGLFYPNEEKVYEIPFTLADMASETPTPVNGSK
Sbjct: 301 RPEDPLDVFLFALFNENQKPGPTSERNYGLFYPNEEKVYEIPFTLADMASETPTPVNGSK 360

Query: 361 AQVPPSKGDTWCVANGNAAAEKLREGLDYACGEGGADCRAIQPGSTCYNPNSLEAHASYA 420
           AQVPPSKGDTWCVANGNAAAEKLREGLDYACGEGGADCRAIQPGSTCYNPNSLEAHASYA
Sbjct: 361 AQVPPSKGDTWCVANGNAAAEKLREGLDYACGEGGADCRAIQPGSTCYNPNSLEAHASYA 420

Query: 421 FNSYYQKMGRASGTCYFGGVAYVVTQPPQYGNCEFPTGY 460
           FNSYYQKMGRASGTCYFGGVAYVVTQPPQYGNCEFPTGY
Sbjct: 421 FNSYYQKMGRASGTCYFGGVAYVVTQPPQYGNCEFPTGY 459

[Close]

BLAST of CmaCh06G008720 vs. ExPASy TrEMBL
Match: A0A6J1GIP4 ((1->3)-beta-glucan endohydrolase OS=Cucurbita moschata OX=3662 GN=LOC111454517 PE=3 SV=1)

HSP 1 Score: 892.5 bits (2305), Expect = 7.4e-256
Identity = 444/459 (96.73%), Postives = 451/459 (98.26%), Query Frame = 0


Query: 1   MAMPFFPFLYLLFSATAHGEPGLIGVNYGRIADNLPPPDKVVHLLKQQGIDRIKVYDTDA 60
           MA+ F PFL+LLFSATA GEPGLIGVNYGRIADNLPPPDKVV LLKQQGIDRIKVYDTDA
Sbjct: 1   MAIAFSPFLFLLFSATALGEPGLIGVNYGRIADNLPPPDKVVGLLKQQGIDRIKVYDTDA 60

Query: 61  AVLTALSNSNISIVVALPNHLLSSAAADSSFTDNWVQSKISNYYPATKINAVAVGNEVFV 120
           AVLTALSNSNISIVVAL N LLSSAAADSSFTDNWVQSKISNYYPATKINAVAVGNEVFV
Sbjct: 61  AVLTALSNSNISIVVALSNDLLSSAAADSSFTDNWVQSKISNYYPATKINAVAVGNEVFV 120

Query: 121 DPNNTTNFLIPAMKNVYSSLQKYNLHDSIKVSSPIALTALASSYPTSSGSFKPDLIEPVI 180
           DPNNTTNFLIPAM+NVYSSLQKYNLHDSIKVSSPIALTALASSYPTSSGSFKPDLIEPVI
Sbjct: 121 DPNNTTNFLIPAMRNVYSSLQKYNLHDSIKVSSPIALTALASSYPTSSGSFKPDLIEPVI 180

Query: 181 KPMLDFLHQTESYLMVNAYPFFAYAANSDQISLDYALFRENSGVVDPGNGLKYFSLFEAQ 240
           KPMLDFLHQTESYLMVNAYPFFAYAANSDQISLDYALFRENSGVVDPGNGLKYFSLFEAQ
Sbjct: 181 KPMLDFLHQTESYLMVNAYPFFAYAANSDQISLDYALFRENSGVVDPGNGLKYFSLFEAQ 240

Query: 241 VDAVYAAMMALKYDDIKVVVTETGWPSIGEEKEVGASKENAASYNGNLVRRVLTGRGTPL 300
           VDAVYAAMMALKYDD+KVVVTETGWPSIGEEKEVGASKENAASYNGNLVRRVLTG+GTPL
Sbjct: 241 VDAVYAAMMALKYDDVKVVVTETGWPSIGEEKEVGASKENAASYNGNLVRRVLTGKGTPL 300

Query: 301 RPEDPLDVFLFALFNENQKPGPTSERNYGLFYPNEEKVYEIPFTLADMASETPTPVNGSK 360
           RPEDPLDVFLFALFNENQKPGPTSERNYGLFYPNEE+VY+IPFTLADMASETPTPVNGSK
Sbjct: 301 RPEDPLDVFLFALFNENQKPGPTSERNYGLFYPNEEEVYQIPFTLADMASETPTPVNGSK 360

Query: 361 AQVPPSKGDTWCVANGNAAAEKLREGLDYACGEGGADCRAIQPGSTCYNPNSLEAHASYA 420
           AQVPPSKGDTWCVANGNAAAEKLREGLDYACGEGGADC AIQPGSTCYNPNSLEAHASYA
Sbjct: 361 AQVPPSKGDTWCVANGNAAAEKLREGLDYACGEGGADCGAIQPGSTCYNPNSLEAHASYA 420

Query: 421 FNSYYQKMGRASGTCYFGGVAYVVTQPPQYGNCEFPTGY 460
           FNSYYQKMGRASGTCYFGGVAYVV QPPQYGNCEFPTGY
Sbjct: 421 FNSYYQKMGRASGTCYFGGVAYVVAQPPQYGNCEFPTGY 459

[Close]

BLAST of CmaCh06G008720 vs. ExPASy TrEMBL
Match: A0A6J1HJA3 ((1->3)-beta-glucan endohydrolase OS=Cucurbita maxima OX=3661 GN=LOC111465026 PE=3 SV=1)

HSP 1 Score: 754.6 bits (1947), Expect = 2.4e-214
Identity = 376/461 (81.56%), Postives = 416/461 (90.24%), Query Frame = 0


Query: 3   MPFFP---FLYLLFSATAHGE-PGLIGVNYGRIADNLPPPDKVVHLLKQQGIDRIKVYDT 62
           M FF    +L LL S+ A  E  G +GVNYGRIAD+LP PDKVV LLK QGIDRIK+YDT
Sbjct: 1   MAFFSSSFYLLLLLSSIALSEAAGWVGVNYGRIADDLPSPDKVVKLLKDQGIDRIKLYDT 60

Query: 63  DAAVLTALSNSNISIVVALPNHLLSSAAADSSFTDNWVQSKISNYYPATKINAVAVGNEV 122
           D+AVLTAL+NSNIS+VVALPN LLSSAAADSSFTD+WVQS IS++YP+TKI+A+AVGNEV
Sbjct: 61  DSAVLTALANSNISVVVALPNELLSSAAADSSFTDSWVQSSISHFYPSTKIDAIAVGNEV 120

Query: 123 FVDPNNTTNFLIPAMKNVYSSLQKYNLHDSIKVSSPIALTALASSYPTSSGSFKPDLIEP 182
           FVDPNNTT FL+PAMKNVY+SLQKYNLH SIK+SSPIALTALASS+PTSSGSFK DLIEP
Sbjct: 121 FVDPNNTTKFLVPAMKNVYTSLQKYNLHSSIKISSPIALTALASSFPTSSGSFKQDLIEP 180

Query: 183 VIKPMLDFLHQTESYLMVNAYPFFAYAANSDQISLDYALFRENSGVVDPGNGLKYFSLFE 242
           VIKPML FL QT+SYLMVNAYPFFAYA+NSD+ISLDYALF++N GVVD GNGLKY SLFE
Sbjct: 181 VIKPMLAFLRQTQSYLMVNAYPFFAYASNSDKISLDYALFQDNPGVVDSGNGLKYSSLFE 240

Query: 243 AQVDAVYAAMMALKYDDIKVVVTETGWPSIGEEKEVGASKENAASYNGNLVRRVLTGRGT 302
           AQ+DAV+AAM ALKYDD+KVVVTETGWPS G+EK+VGAS+ENAASYNGNLV+RVLTG GT
Sbjct: 241 AQIDAVFAAMSALKYDDVKVVVTETGWPSKGDEKDVGASEENAASYNGNLVKRVLTGSGT 300

Query: 303 PLRPEDPLDVFLFALFNENQKPGPTSERNYGLFYPNEEKVYEIPFTLADMASETPTPVN- 362
           PLRP DPL+VFLFALFNENQKPGPTSERNYGLFYPN+++VY+IP T AD+ SE PT VN 
Sbjct: 301 PLRPNDPLNVFLFALFNENQKPGPTSERNYGLFYPNQQRVYDIPLTRADVESEAPTMVNG 360

Query: 363 GSKAQVPPSKGDTWCVANGNAAAEKLREGLDYACGEGGADCRAIQPGSTCYNPNSLEAHA 422
           GSKAQVP SKG TWCVANG  AAE+L+ GLDYACGEGGADCR IQPGSTCYNPNS+EAHA
Sbjct: 361 GSKAQVPSSKGQTWCVANGKTAAERLQAGLDYACGEGGADCRPIQPGSTCYNPNSVEAHA 420

Query: 423 SYAFNSYYQKMGRASGTCYFGGVAYVVTQPPQYGNCEFPTG 459
           SYAFNSYYQKMGRASGTC FGG AYVV+QPPQYG CEFPTG
Sbjct: 421 SYAFNSYYQKMGRASGTCEFGGAAYVVSQPPQYGKCEFPTG 461

[Close]

BLAST of CmaCh06G008720 vs. ExPASy TrEMBL
Match: A0A6J1EW84 ((1->3)-beta-glucan endohydrolase OS=Cucurbita moschata OX=3662 GN=LOC111438639 PE=3 SV=1)

HSP 1 Score: 750.0 bits (1935), Expect = 6.0e-213
Identity = 373/460 (81.09%), Postives = 414/460 (90.00%), Query Frame = 0


Query: 3   MPFF--PFLYLLFSATAHGE-PGLIGVNYGRIADNLPPPDKVVHLLKQQGIDRIKVYDTD 62
           M FF   F  LL S+ A  E  G +GVNYGRIAD+LPPP+KVV LLK QGIDRIK+YDTD
Sbjct: 1   MAFFSSSFYLLLLSSIALSEAAGWVGVNYGRIADDLPPPEKVVKLLKDQGIDRIKLYDTD 60

Query: 63  AAVLTALSNSNISIVVALPNHLLSSAAADSSFTDNWVQSKISNYYPATKINAVAVGNEVF 122
           + VLTAL+NSNIS+VVALPN LLSSAAADSSFTDNWVQS IS++YP+TKI+A+AVGNEVF
Sbjct: 61  STVLTALANSNISVVVALPNELLSSAAADSSFTDNWVQSSISHFYPSTKIDAIAVGNEVF 120

Query: 123 VDPNNTTNFLIPAMKNVYSSLQKYNLHDSIKVSSPIALTALASSYPTSSGSFKPDLIEPV 182
           VDPNNTT FL+PAMKNVY+SLQKYNLH+SIK+SSPIALTALASS+PTSSGSFK DLIEPV
Sbjct: 121 VDPNNTTKFLVPAMKNVYTSLQKYNLHNSIKISSPIALTALASSFPTSSGSFKQDLIEPV 180

Query: 183 IKPMLDFLHQTESYLMVNAYPFFAYAANSDQISLDYALFRENSGVVDPGNGLKYFSLFEA 242
           IKPML FL +T+SYLM+NAYPFFAYA+NSD+ISLDYALFR+N GV+D GNGLKY SLFEA
Sbjct: 181 IKPMLAFLRKTQSYLMINAYPFFAYASNSDKISLDYALFRDNPGVLDSGNGLKYSSLFEA 240

Query: 243 QVDAVYAAMMALKYDDIKVVVTETGWPSIGEEKEVGASKENAASYNGNLVRRVLTGRGTP 302
           Q+DAV+AAM ALKYDD+KVVVTETGWPS G+EK+VGAS ENAASYNGNLV+RVLTG GTP
Sbjct: 241 QIDAVFAAMSALKYDDVKVVVTETGWPSKGDEKDVGASGENAASYNGNLVKRVLTGSGTP 300

Query: 303 LRPEDPLDVFLFALFNENQKPGPTSERNYGLFYPNEEKVYEIPFTLADMASETPTPVN-G 362
           LRP DPL+VFLFALFNENQKPGPTSERNYGLFYPN+++VY+IP T AD+ S +PT VN G
Sbjct: 301 LRPNDPLNVFLFALFNENQKPGPTSERNYGLFYPNQQRVYDIPLTRADVESGSPTMVNGG 360

Query: 363 SKAQVPPSKGDTWCVANGNAAAEKLREGLDYACGEGGADCRAIQPGSTCYNPNSLEAHAS 422
           SKAQVP SKG TWCVANG  AAEKL+ GLDYACGEGGADCR IQPGSTCYNPNS+EAHAS
Sbjct: 361 SKAQVPSSKGQTWCVANGKTAAEKLQAGLDYACGEGGADCRPIQPGSTCYNPNSVEAHAS 420

Query: 423 YAFNSYYQKMGRASGTCYFGGVAYVVTQPPQYGNCEFPTG 459
           YAFNSYYQKM RASGTC FGG AYVV+QPPQYG CEFPTG
Sbjct: 421 YAFNSYYQKMARASGTCDFGGAAYVVSQPPQYGKCEFPTG 460

[Close]

BLAST of CmaCh06G008720 vs. ExPASy TrEMBL
Match: A0A5D3DKE2 ((1->3)-beta-glucan endohydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold943G00450 PE=3 SV=1)

HSP 1 Score: 694.5 bits (1791), Expect = 3.0e-196
Identity = 343/457 (75.05%), Postives = 393/457 (86.00%), Query Frame = 0


Query: 3   MPFFPFL--YLLFSATAHGEPGLIGVNYGRIADNLPPPDKVVHLLKQQGIDRIKVYDTDA 62
           M FF  L  +LL S+T       +GVNYGRIADNLP PDKVV LLK  GIDRIK++DT++
Sbjct: 1   MAFFSSLSFFLLLSSTLSHAHAWVGVNYGRIADNLPSPDKVVQLLKSHGIDRIKLFDTES 60

Query: 63  AVLTALSNSNISIVVALPNHLLSSAAADSSFTDNWVQSKISNYYPATKINAVAVGNEVFV 122
            VL+ALSNSNIS+VVALPN  LSSAAAD SFTDNWVQS IS++YP+TKI+A+AVGNEVFV
Sbjct: 61  TVLSALSNSNISVVVALPNEHLSSAAADPSFTDNWVQSNISHFYPSTKIDAIAVGNEVFV 120

Query: 123 DPNNTTNFLIPAMKNVYSSLQKYNLHDSIKVSSPIALTALASSYPTSSGSFKPDLIEPVI 182
           DPNNTT FL+PAM+NVY+SLQK+NLH++IKVSSPIAL++LASSYPTSSGSF+PDLIEPVI
Sbjct: 121 DPNNTTKFLVPAMQNVYASLQKFNLHNNIKVSSPIALSSLASSYPTSSGSFRPDLIEPVI 180

Query: 183 KPMLDFLHQTESYLMVNAYPFFAYAANSDQISLDYALFRENSGVVDPGNGLKYFSLFEAQ 242
           KPMLD L Q++SYLMVN YPFFAY  N+D+IS+DYALFREN GVVD GNGLKY +LFEAQ
Sbjct: 181 KPMLDLLRQSQSYLMVNVYPFFAYVGNADKISIDYALFRENPGVVDSGNGLKYSNLFEAQ 240

Query: 243 VDAVYAAMMALKYDDIKVVVTETGWPSIGEEKEVGASKENAASYNGNLVRRVLTGRGTPL 302
           VDAV+AAM A+KYDD+ +VVTETGWPS GEE E+GAS ENAA+YNGNLV+RVLTG GTPL
Sbjct: 241 VDAVFAAMSAIKYDDVPLVVTETGWPSQGEENEIGASTENAAAYNGNLVKRVLTGSGTPL 300

Query: 303 RPEDPLDVFLFALFNENQKPGPTSERNYGLFYPNEEKVYEIPFTLADMASETPTPVNGSK 362
           RP+DPL+V+LFALFNENQKPGPTSERNYGLFYPNEEKVYEI  T  D+   TP   NGSK
Sbjct: 301 RPKDPLNVYLFALFNENQKPGPTSERNYGLFYPNEEKVYEISLT-GDVVGATPPDSNGSK 360

Query: 363 AQVPPSKGDTWCVANGNAAAEKLREGLDYACGEGGADCRAIQPGSTCYNPNSLEAHASYA 422
            +V    G TWCVAN NA A+KL+ GLDYACGEG ADCR+IQ G  CY+PNSL+AHASYA
Sbjct: 361 VEV---TGQTWCVANENAGADKLQAGLDYACGEGQADCRSIQQGGACYDPNSLQAHASYA 420

Query: 423 FNSYYQKMGRASGTCYFGGVAYVVTQPPQYGNCEFPT 458
           FNSYYQKM RA+GTC FGG AY+VTQPPQYG C+FP+
Sbjct: 421 FNSYYQKMKRAAGTCDFGGAAYIVTQPPQYGKCDFPS 453

[Close]

BLAST of CmaCh06G008720 vs. NCBI nr
Match: XP_023005925.1 (glucan endo-1,3-beta-glucosidase 12-like [Cucurbita maxima])

HSP 1 Score: 926.4 bits (2393), Expect = 9.6e-266
Identity = 459/459 (100.00%), Postives = 459/459 (100.00%), Query Frame = 0


Query: 1   MAMPFFPFLYLLFSATAHGEPGLIGVNYGRIADNLPPPDKVVHLLKQQGIDRIKVYDTDA 60
           MAMPFFPFLYLLFSATAHGEPGLIGVNYGRIADNLPPPDKVVHLLKQQGIDRIKVYDTDA
Sbjct: 1   MAMPFFPFLYLLFSATAHGEPGLIGVNYGRIADNLPPPDKVVHLLKQQGIDRIKVYDTDA 60

Query: 61  AVLTALSNSNISIVVALPNHLLSSAAADSSFTDNWVQSKISNYYPATKINAVAVGNEVFV 120
           AVLTALSNSNISIVVALPNHLLSSAAADSSFTDNWVQSKISNYYPATKINAVAVGNEVFV
Sbjct: 61  AVLTALSNSNISIVVALPNHLLSSAAADSSFTDNWVQSKISNYYPATKINAVAVGNEVFV 120

Query: 121 DPNNTTNFLIPAMKNVYSSLQKYNLHDSIKVSSPIALTALASSYPTSSGSFKPDLIEPVI 180
           DPNNTTNFLIPAMKNVYSSLQKYNLHDSIKVSSPIALTALASSYPTSSGSFKPDLIEPVI
Sbjct: 121 DPNNTTNFLIPAMKNVYSSLQKYNLHDSIKVSSPIALTALASSYPTSSGSFKPDLIEPVI 180

Query: 181 KPMLDFLHQTESYLMVNAYPFFAYAANSDQISLDYALFRENSGVVDPGNGLKYFSLFEAQ 240
           KPMLDFLHQTESYLMVNAYPFFAYAANSDQISLDYALFRENSGVVDPGNGLKYFSLFEAQ
Sbjct: 181 KPMLDFLHQTESYLMVNAYPFFAYAANSDQISLDYALFRENSGVVDPGNGLKYFSLFEAQ 240

Query: 241 VDAVYAAMMALKYDDIKVVVTETGWPSIGEEKEVGASKENAASYNGNLVRRVLTGRGTPL 300
           VDAVYAAMMALKYDDIKVVVTETGWPSIGEEKEVGASKENAASYNGNLVRRVLTGRGTPL
Sbjct: 241 VDAVYAAMMALKYDDIKVVVTETGWPSIGEEKEVGASKENAASYNGNLVRRVLTGRGTPL 300

Query: 301 RPEDPLDVFLFALFNENQKPGPTSERNYGLFYPNEEKVYEIPFTLADMASETPTPVNGSK 360
           RPEDPLDVFLFALFNENQKPGPTSERNYGLFYPNEEKVYEIPFTLADMASETPTPVNGSK
Sbjct: 301 RPEDPLDVFLFALFNENQKPGPTSERNYGLFYPNEEKVYEIPFTLADMASETPTPVNGSK 360

Query: 361 AQVPPSKGDTWCVANGNAAAEKLREGLDYACGEGGADCRAIQPGSTCYNPNSLEAHASYA 420
           AQVPPSKGDTWCVANGNAAAEKLREGLDYACGEGGADCRAIQPGSTCYNPNSLEAHASYA
Sbjct: 361 AQVPPSKGDTWCVANGNAAAEKLREGLDYACGEGGADCRAIQPGSTCYNPNSLEAHASYA 420

Query: 421 FNSYYQKMGRASGTCYFGGVAYVVTQPPQYGNCEFPTGY 460
           FNSYYQKMGRASGTCYFGGVAYVVTQPPQYGNCEFPTGY
Sbjct: 421 FNSYYQKMGRASGTCYFGGVAYVVTQPPQYGNCEFPTGY 459

[Close]

BLAST of CmaCh06G008720 vs. NCBI nr
Match: KAG7028424.1 (Glucan endo-1,3-beta-glucosidase 13, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 893.3 bits (2307), Expect = 9.0e-256
Identity = 444/459 (96.73%), Postives = 452/459 (98.47%), Query Frame = 0


Query: 1   MAMPFFPFLYLLFSATAHGEPGLIGVNYGRIADNLPPPDKVVHLLKQQGIDRIKVYDTDA 60
           MA+ F PFL+LLF+ATA GEPGLIGVNYGRIADNLPPPDKVV LLKQQGIDRIKVYDTDA
Sbjct: 1   MAIAFSPFLFLLFAATALGEPGLIGVNYGRIADNLPPPDKVVDLLKQQGIDRIKVYDTDA 60

Query: 61  AVLTALSNSNISIVVALPNHLLSSAAADSSFTDNWVQSKISNYYPATKINAVAVGNEVFV 120
           AVLTALSNSNISIVVAL N LLSSAAADSSFTDNWVQSKISNYYPATKINAVAVGNEVFV
Sbjct: 61  AVLTALSNSNISIVVALSNDLLSSAAADSSFTDNWVQSKISNYYPATKINAVAVGNEVFV 120

Query: 121 DPNNTTNFLIPAMKNVYSSLQKYNLHDSIKVSSPIALTALASSYPTSSGSFKPDLIEPVI 180
           DPNNTTNFLIPAMKNVYSSLQKYNLHDSIKVSSPIALTALASSYPTSSGSFKPDLIEPVI
Sbjct: 121 DPNNTTNFLIPAMKNVYSSLQKYNLHDSIKVSSPIALTALASSYPTSSGSFKPDLIEPVI 180

Query: 181 KPMLDFLHQTESYLMVNAYPFFAYAANSDQISLDYALFRENSGVVDPGNGLKYFSLFEAQ 240
           KPMLDFLH+TESYLMVNAYPFFAYAANSDQISLDYALFRENSGVVDPGNGLKYFSLFEAQ
Sbjct: 181 KPMLDFLHRTESYLMVNAYPFFAYAANSDQISLDYALFRENSGVVDPGNGLKYFSLFEAQ 240

Query: 241 VDAVYAAMMALKYDDIKVVVTETGWPSIGEEKEVGASKENAASYNGNLVRRVLTGRGTPL 300
           VDAVYAAMMALKYDD+KVVVTETGWPSIGEEKEVGASKENAASYNGNLVRRVLTG+GTPL
Sbjct: 241 VDAVYAAMMALKYDDVKVVVTETGWPSIGEEKEVGASKENAASYNGNLVRRVLTGKGTPL 300

Query: 301 RPEDPLDVFLFALFNENQKPGPTSERNYGLFYPNEEKVYEIPFTLADMASETPTPVNGSK 360
           RPEDPLDVFLFALFNENQKPGPTSERNYGLFYPNEE+VY+IPFTLADMASETPTPVNGSK
Sbjct: 301 RPEDPLDVFLFALFNENQKPGPTSERNYGLFYPNEEEVYQIPFTLADMASETPTPVNGSK 360

Query: 361 AQVPPSKGDTWCVANGNAAAEKLREGLDYACGEGGADCRAIQPGSTCYNPNSLEAHASYA 420
           AQVPPSKGDTWCVANGNAAAEKLREGLDYACGEGGADCRAIQPGSTCYNPNSLEAHASYA
Sbjct: 361 AQVPPSKGDTWCVANGNAAAEKLREGLDYACGEGGADCRAIQPGSTCYNPNSLEAHASYA 420

Query: 421 FNSYYQKMGRASGTCYFGGVAYVVTQPPQYGNCEFPTGY 460
           FNSYYQKMGRASGTCYFGGVAYVVTQPP YGNCEFPTGY
Sbjct: 421 FNSYYQKMGRASGTCYFGGVAYVVTQPPLYGNCEFPTGY 459

[Close]

BLAST of CmaCh06G008720 vs. NCBI nr
Match: XP_022951783.1 (glucan endo-1,3-beta-glucosidase 12-like [Cucurbita moschata])

HSP 1 Score: 892.5 bits (2305), Expect = 1.5e-255
Identity = 444/459 (96.73%), Postives = 451/459 (98.26%), Query Frame = 0


Query: 1   MAMPFFPFLYLLFSATAHGEPGLIGVNYGRIADNLPPPDKVVHLLKQQGIDRIKVYDTDA 60
           MA+ F PFL+LLFSATA GEPGLIGVNYGRIADNLPPPDKVV LLKQQGIDRIKVYDTDA
Sbjct: 1   MAIAFSPFLFLLFSATALGEPGLIGVNYGRIADNLPPPDKVVGLLKQQGIDRIKVYDTDA 60

Query: 61  AVLTALSNSNISIVVALPNHLLSSAAADSSFTDNWVQSKISNYYPATKINAVAVGNEVFV 120
           AVLTALSNSNISIVVAL N LLSSAAADSSFTDNWVQSKISNYYPATKINAVAVGNEVFV
Sbjct: 61  AVLTALSNSNISIVVALSNDLLSSAAADSSFTDNWVQSKISNYYPATKINAVAVGNEVFV 120

Query: 121 DPNNTTNFLIPAMKNVYSSLQKYNLHDSIKVSSPIALTALASSYPTSSGSFKPDLIEPVI 180
           DPNNTTNFLIPAM+NVYSSLQKYNLHDSIKVSSPIALTALASSYPTSSGSFKPDLIEPVI
Sbjct: 121 DPNNTTNFLIPAMRNVYSSLQKYNLHDSIKVSSPIALTALASSYPTSSGSFKPDLIEPVI 180

Query: 181 KPMLDFLHQTESYLMVNAYPFFAYAANSDQISLDYALFRENSGVVDPGNGLKYFSLFEAQ 240
           KPMLDFLHQTESYLMVNAYPFFAYAANSDQISLDYALFRENSGVVDPGNGLKYFSLFEAQ
Sbjct: 181 KPMLDFLHQTESYLMVNAYPFFAYAANSDQISLDYALFRENSGVVDPGNGLKYFSLFEAQ 240

Query: 241 VDAVYAAMMALKYDDIKVVVTETGWPSIGEEKEVGASKENAASYNGNLVRRVLTGRGTPL 300
           VDAVYAAMMALKYDD+KVVVTETGWPSIGEEKEVGASKENAASYNGNLVRRVLTG+GTPL
Sbjct: 241 VDAVYAAMMALKYDDVKVVVTETGWPSIGEEKEVGASKENAASYNGNLVRRVLTGKGTPL 300

Query: 301 RPEDPLDVFLFALFNENQKPGPTSERNYGLFYPNEEKVYEIPFTLADMASETPTPVNGSK 360
           RPEDPLDVFLFALFNENQKPGPTSERNYGLFYPNEE+VY+IPFTLADMASETPTPVNGSK
Sbjct: 301 RPEDPLDVFLFALFNENQKPGPTSERNYGLFYPNEEEVYQIPFTLADMASETPTPVNGSK 360

Query: 361 AQVPPSKGDTWCVANGNAAAEKLREGLDYACGEGGADCRAIQPGSTCYNPNSLEAHASYA 420
           AQVPPSKGDTWCVANGNAAAEKLREGLDYACGEGGADC AIQPGSTCYNPNSLEAHASYA
Sbjct: 361 AQVPPSKGDTWCVANGNAAAEKLREGLDYACGEGGADCGAIQPGSTCYNPNSLEAHASYA 420

Query: 421 FNSYYQKMGRASGTCYFGGVAYVVTQPPQYGNCEFPTGY 460
           FNSYYQKMGRASGTCYFGGVAYVV QPPQYGNCEFPTGY
Sbjct: 421 FNSYYQKMGRASGTCYFGGVAYVVAQPPQYGNCEFPTGY 459

[Close]

BLAST of CmaCh06G008720 vs. NCBI nr
Match: KAG6596945.1 (Glucan endo-1,3-beta-glucosidase 13, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 891.3 bits (2302), Expect = 3.4e-255
Identity = 443/459 (96.51%), Postives = 451/459 (98.26%), Query Frame = 0


Query: 1   MAMPFFPFLYLLFSATAHGEPGLIGVNYGRIADNLPPPDKVVHLLKQQGIDRIKVYDTDA 60
           MA+ F PFL+LLF+ATA GEPGLIGVNYGRIADNLPPPDKVV LLKQQGIDRIKVYDTDA
Sbjct: 1   MAIAFSPFLFLLFAATALGEPGLIGVNYGRIADNLPPPDKVVDLLKQQGIDRIKVYDTDA 60

Query: 61  AVLTALSNSNISIVVALPNHLLSSAAADSSFTDNWVQSKISNYYPATKINAVAVGNEVFV 120
           AVLTALSNSNISIVVAL N LLSSAAADSSFTDNWVQSKISNYYPATKINAVAVGNEVFV
Sbjct: 61  AVLTALSNSNISIVVALSNDLLSSAAADSSFTDNWVQSKISNYYPATKINAVAVGNEVFV 120

Query: 121 DPNNTTNFLIPAMKNVYSSLQKYNLHDSIKVSSPIALTALASSYPTSSGSFKPDLIEPVI 180
           DPNNTTNFLIPAMKNVYSSLQKYNLHDSIKVSSPIALTALASSYPTSSGSFKPDLIEPVI
Sbjct: 121 DPNNTTNFLIPAMKNVYSSLQKYNLHDSIKVSSPIALTALASSYPTSSGSFKPDLIEPVI 180

Query: 181 KPMLDFLHQTESYLMVNAYPFFAYAANSDQISLDYALFRENSGVVDPGNGLKYFSLFEAQ 240
           KPMLDFLH+TESYLMVNAYPFFAYAANSDQISLDYALFRENSGVVDPGNGLKYFSLFEAQ
Sbjct: 181 KPMLDFLHRTESYLMVNAYPFFAYAANSDQISLDYALFRENSGVVDPGNGLKYFSLFEAQ 240

Query: 241 VDAVYAAMMALKYDDIKVVVTETGWPSIGEEKEVGASKENAASYNGNLVRRVLTGRGTPL 300
           VDAVYAAMMALKYDD+KVVVTETGWPSIGEEKEVGASKENAASYNGNLVRRVLTG+GTPL
Sbjct: 241 VDAVYAAMMALKYDDVKVVVTETGWPSIGEEKEVGASKENAASYNGNLVRRVLTGKGTPL 300

Query: 301 RPEDPLDVFLFALFNENQKPGPTSERNYGLFYPNEEKVYEIPFTLADMASETPTPVNGSK 360
           RPEDPLDVFLFALFNENQKPGPTSERNYGLFYPNEE+VY+IPFTLADMASETPTPVNGSK
Sbjct: 301 RPEDPLDVFLFALFNENQKPGPTSERNYGLFYPNEEEVYQIPFTLADMASETPTPVNGSK 360

Query: 361 AQVPPSKGDTWCVANGNAAAEKLREGLDYACGEGGADCRAIQPGSTCYNPNSLEAHASYA 420
           AQVPPSKGDTWCVANGNAAAEKLREGLDYACGEGGADC AIQPGSTCYNPNSLEAHASYA
Sbjct: 361 AQVPPSKGDTWCVANGNAAAEKLREGLDYACGEGGADCHAIQPGSTCYNPNSLEAHASYA 420

Query: 421 FNSYYQKMGRASGTCYFGGVAYVVTQPPQYGNCEFPTGY 460
           FNSYYQKMGRASGTCYFGGVAYVVTQPP YGNCEFPTGY
Sbjct: 421 FNSYYQKMGRASGTCYFGGVAYVVTQPPLYGNCEFPTGY 459

[Close]

BLAST of CmaCh06G008720 vs. NCBI nr
Match: XP_023520756.1 (glucan endo-1,3-beta-glucosidase 12-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 891.0 bits (2301), Expect = 4.5e-255
Identity = 443/459 (96.51%), Postives = 450/459 (98.04%), Query Frame = 0


Query: 1   MAMPFFPFLYLLFSATAHGEPGLIGVNYGRIADNLPPPDKVVHLLKQQGIDRIKVYDTDA 60
           M + F PFL+LLFSATA G+PGLIGVNYGRIADNLPPP KVV LLKQQGI RIKVYDTDA
Sbjct: 1   MPIAFSPFLFLLFSATALGDPGLIGVNYGRIADNLPPPHKVVDLLKQQGIHRIKVYDTDA 60

Query: 61  AVLTALSNSNISIVVALPNHLLSSAAADSSFTDNWVQSKISNYYPATKINAVAVGNEVFV 120
           AVLTALSNSNISIVVAL NHLLSSAAADSSFTDNWVQSKISNYYPATKINAVAVGNEVFV
Sbjct: 61  AVLTALSNSNISIVVALSNHLLSSAAADSSFTDNWVQSKISNYYPATKINAVAVGNEVFV 120

Query: 121 DPNNTTNFLIPAMKNVYSSLQKYNLHDSIKVSSPIALTALASSYPTSSGSFKPDLIEPVI 180
           DPNNTTNFLIPAMKNVYSSLQKYNLHDSIKVSSPIALTALASSYPTSSGSFKPDLIEPVI
Sbjct: 121 DPNNTTNFLIPAMKNVYSSLQKYNLHDSIKVSSPIALTALASSYPTSSGSFKPDLIEPVI 180

Query: 181 KPMLDFLHQTESYLMVNAYPFFAYAANSDQISLDYALFRENSGVVDPGNGLKYFSLFEAQ 240
           KPMLDFLHQTESYLMVNAYPFFAYAANSDQISLDYALFRENSGVVDPGNGLKYFSLFEAQ
Sbjct: 181 KPMLDFLHQTESYLMVNAYPFFAYAANSDQISLDYALFRENSGVVDPGNGLKYFSLFEAQ 240

Query: 241 VDAVYAAMMALKYDDIKVVVTETGWPSIGEEKEVGASKENAASYNGNLVRRVLTGRGTPL 300
           VDAVYAAMMALKYDD+KVVVTETGWPSIGEEKEVGASKENAASYNGNLVRRVLTGRGTPL
Sbjct: 241 VDAVYAAMMALKYDDVKVVVTETGWPSIGEEKEVGASKENAASYNGNLVRRVLTGRGTPL 300

Query: 301 RPEDPLDVFLFALFNENQKPGPTSERNYGLFYPNEEKVYEIPFTLADMASETPTPVNGSK 360
           RPEDPLDVFLFALFNENQKPGPTSERNYGLFYPNEE+VY+IPFTLADMASETPTP+NGSK
Sbjct: 301 RPEDPLDVFLFALFNENQKPGPTSERNYGLFYPNEEEVYQIPFTLADMASETPTPMNGSK 360

Query: 361 AQVPPSKGDTWCVANGNAAAEKLREGLDYACGEGGADCRAIQPGSTCYNPNSLEAHASYA 420
           AQVPPSKGDTWCVANGNAAAEKLREGLDYACGEGGADC AIQPGSTCYNPNSLEAHASYA
Sbjct: 361 AQVPPSKGDTWCVANGNAAAEKLREGLDYACGEGGADCGAIQPGSTCYNPNSLEAHASYA 420

Query: 421 FNSYYQKMGRASGTCYFGGVAYVVTQPPQYGNCEFPTGY 460
           FNSYYQKMGRASGTCYFGGVAYVVTQPPQYGNCEFPTGY
Sbjct: 421 FNSYYQKMGRASGTCYFGGVAYVVTQPPQYGNCEFPTGY 459

[Close]

BLAST of CmaCh06G008720 vs. TAIR 10
Match: AT4G26830.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 643.7 bits (1659), Expect = 1.2e-184
Identity = 314/461 (68.11%), Postives = 383/461 (83.08%), Query Frame = 0


Query: 1   MAMPFFP-FLYLLFSATAHGEPGLIGVNYGRIADNLPPPDKVVHLLKQQGIDRIKVYDTD 60
           MA+ F P FL L F +      G++GVNYGRIA+NLP P+KVV+LLK QGI+RIK++DTD
Sbjct: 1   MAVSFLPYFLILSFLSAIDAHSGMVGVNYGRIANNLPSPEKVVNLLKSQGINRIKIFDTD 60

Query: 61  AAVLTALSNSNISIVVALPNHLLSSAAADSSFTDNWVQSKISNYYPATKINAVAVGNEVF 120
             VLTAL+NS I ++VALPN LLSSAA+  SF DNW+++ I  Y+PAT+I A+AVGNEVF
Sbjct: 61  KNVLTALANSKIKVIVALPNELLSSAASHQSFADNWIKTHIMPYFPATEIEAIAVGNEVF 120

Query: 121 VDPNNTTNFLIPAMKNVYSSLQKYNLHDSIKVSSPIALTALASSYPTSSGSFKPDLIEPV 180
           VDP   T +L+ AMKN+++SL KY L  +IK+SSPIAL+ALA+SYP SSGSFKP+LIEPV
Sbjct: 121 VDP-TITPYLVNAMKNIHTSLVKYKLDKAIKISSPIALSALANSYPPSSGSFKPELIEPV 180

Query: 181 IKPMLDFLHQTESYLMVNAYPFFAYAANSDQISLDYALFRENSGVVDPGNGLKYFSLFEA 240
           +KPML  L QT SYLMVNAYPFFAYAAN+D+ISLDYALF+EN+G +D G GLKY SLF+A
Sbjct: 181 VKPMLALLQQTSSYLMVNAYPFFAYAANADKISLDYALFKENAGNIDSGTGLKYNSLFDA 240

Query: 241 QVDAVYAAMMALKYDDIKVVVTETGWPSIGEEKEVGASKENAASYNGNLVRRVLTGRGTP 300
           Q+DAVYAA+ A+ +  +KV+VTETGWPS+G+E E+GAS+ NAA+YN  LV+RVLTG+GTP
Sbjct: 241 QIDAVYAALSAVGFKGVKVMVTETGWPSVGDENEIGASESNAAAYNAGLVKRVLTGKGTP 300

Query: 301 LRPEDPLDVFLFALFNENQKPGPTSERNYGLFYPNEEKVYEIPFTLADMASETPTPVNGS 360
           LRP +PL+V+LFALFNENQKPGPTSERNYGLFYPNE KVY +PFT      ++ TPVNG+
Sbjct: 301 LRPTEPLNVYLFALFNENQKPGPTSERNYGLFYPNEGKVYNVPFT-----KKSTTPVNGN 360

Query: 361 KAQVPPS-KGDTWCVANGNAAAEKLREGLDYACGEGGADCRAIQPGSTCYNPNSLEAHAS 420
           + +VP + +G TWCV+NG  A EKL+E LDYACGEGGADCR IQPG+TCY+P SLEAHAS
Sbjct: 361 RGKVPVTHEGHTWCVSNGEVAKEKLQEALDYACGEGGADCRPIQPGATCYHPESLEAHAS 420

Query: 421 YAFNSYYQKMGRASGTCYFGGVAYVVTQPPQYGNCEFPTGY 460
           YAFNSYYQK  R  GTC+FGG A+VVTQPP+YG CEFPTG+
Sbjct: 421 YAFNSYYQKNSRRVGTCFFGGAAHVVTQPPRYGKCEFPTGH 455

[Close]

BLAST of CmaCh06G008720 vs. TAIR 10
Match: AT5G55180.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 643.7 bits (1659), Expect = 1.2e-184
Identity = 315/446 (70.63%), Postives = 368/446 (82.51%), Query Frame = 0


Query: 18  HGEPGLIGVNYGRIADNLPPPDKVVHLLKQQGIDRIKVYDTDAAVLTALSNSNISIVVAL 77
           + + G+IGVNYGRIADNLP P+KVV LLK QGI+RIK+YDT+  VLTAL+NS I +VV+L
Sbjct: 21  YADSGMIGVNYGRIADNLPAPEKVVELLKTQGINRIKLYDTETTVLTALANSGIKVVVSL 80

Query: 78  PNHLLSSAAADSSFTDNWVQSKISNYYPATKINAVAVGNEVFVDPNNTTNFLIPAMKNVY 137
           PN  L+SAAAD S+TD WVQ  I  Y PAT I A+AVGNEVFVDP NTT +L+PAMKNV 
Sbjct: 81  PNENLASAAADQSYTDTWVQDNIKKYIPATDIEAIAVGNEVFVDPRNTTTYLVPAMKNVQ 140

Query: 138 SSLQKYNLHDSIKVSSPIALTALASSYPTSSGSFKPDLIEPVIKPMLDFLHQTESYLMVN 197
           SSL K+NL  SIK+SSPIAL+ALASSYP S+GSFKP+LIEPVIKPMLD L +T S+LMVN
Sbjct: 141 SSLVKFNLDKSIKISSPIALSALASSYPPSAGSFKPELIEPVIKPMLDLLRKTSSHLMVN 200

Query: 198 AYPFFAYAANSDQISLDYALFRENSGVVDPGNGLKYFSLFEAQVDAVYAAMMALKYDDIK 257
           AYPFFAYAAN+D+ISLDYALF+EN+G VD GNGLKY SL +AQ+DAV+AAM A+ ++D+K
Sbjct: 201 AYPFFAYAANADKISLDYALFKENAGNVDSGNGLKYNSLLDAQIDAVFAAMSAVGFNDVK 260

Query: 258 VVVTETGWPSIGEEKEVGASKENAASYNGNLVRRVLTGRGTPLRPEDPLDVFLFALFNEN 317
           +VVTETGWPS G+E E+GA   NAA+YNG LV+RVLTG GTPL+P++PL+V+LFALFNEN
Sbjct: 261 LVVTETGWPSAGDENEIGAGSANAAAYNGGLVKRVLTGNGTPLKPKEPLNVYLFALFNEN 320

Query: 318 QKPGPTSERNYGLFYPNEEKVYEIPFTLADMASETPTPVNGSKAQVPPSK----GDTWCV 377
           QK GPTSERNYGLFYPNE KVY++        +   TPVN +K +V P K    G TWCV
Sbjct: 321 QKTGPTSERNYGLFYPNENKVYDVSL------NGKSTPVNDNKEKVVPVKPSLVGQTWCV 380

Query: 378 ANGNAAAEKLREGLDYACGEGGADCRAIQPGSTCYNPNSLEAHASYAFNSYYQKMGRASG 437
           ANG    EKL+EGLDYACGEGGADCR IQPG+TCYNP SLEAHASYAFNSYYQK  R  G
Sbjct: 381 ANGKTTKEKLQEGLDYACGEGGADCRPIQPGATCYNPESLEAHASYAFNSYYQKNARGVG 440

Query: 438 TCYFGGVAYVVTQPPQYGNCEFPTGY 460
           TC FGG AYVV+QPP+YG CEFPTG+
Sbjct: 441 TCNFGGAAYVVSQPPKYGKCEFPTGH 460

[Close]

BLAST of CmaCh06G008720 vs. TAIR 10
Match: AT5G55180.2 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 622.9 bits (1605), Expect = 2.1e-178
Identity = 307/435 (70.57%), Postives = 358/435 (82.30%), Query Frame = 0


Query: 18  HGEPGLIGVNYGRIADNLPPPDKVVHLLKQQGIDRIKVYDTDAAVLTALSNSNISIVVAL 77
           + + G+IGVNYGRIADNLP P+KVV LLK QGI+RIK+YDT+  VLTAL+NS I +VV+L
Sbjct: 21  YADSGMIGVNYGRIADNLPAPEKVVELLKTQGINRIKLYDTETTVLTALANSGIKVVVSL 80

Query: 78  PNHLLSSAAADSSFTDNWVQSKISNYYPATKINAVAVGNEVFVDPNNTTNFLIPAMKNVY 137
           PN  L+SAAAD S+TD WVQ  I  Y PAT I A+AVGNEVFVDP NTT +L+PAMKNV 
Sbjct: 81  PNENLASAAADQSYTDTWVQDNIKKYIPATDIEAIAVGNEVFVDPRNTTTYLVPAMKNVQ 140

Query: 138 SSLQKYNLHDSIKVSSPIALTALASSYPTSSGSFKPDLIEPVIKPMLDFLHQTESYLMVN 197
           SSL K+NL  SIK+SSPIAL+ALASSYP S+GSFKP+LIEPVIKPMLD L +T S+LMVN
Sbjct: 141 SSLVKFNLDKSIKISSPIALSALASSYPPSAGSFKPELIEPVIKPMLDLLRKTSSHLMVN 200

Query: 198 AYPFFAYAANSDQISLDYALFRENSGVVDPGNGLKYFSLFEAQVDAVYAAMMALKYDDIK 257
           AYPFFAYAAN+D+ISLDYALF+EN+G VD GNGLKY SL +AQ+DAV+AAM A+ ++D+K
Sbjct: 201 AYPFFAYAANADKISLDYALFKENAGNVDSGNGLKYNSLLDAQIDAVFAAMSAVGFNDVK 260

Query: 258 VVVTETGWPSIGEEKEVGASKENAASYNGNLVRRVLTGRGTPLRPEDPLDVFLFALFNEN 317
           +VVTETGWPS G+E E+GA   NAA+YNG LV+RVLTG GTPL+P++PL+V+LFALFNEN
Sbjct: 261 LVVTETGWPSAGDENEIGAGSANAAAYNGGLVKRVLTGNGTPLKPKEPLNVYLFALFNEN 320

Query: 318 QKPGPTSERNYGLFYPNEEKVYEIPFTLADMASETPTPVNGSKAQVPPSK----GDTWCV 377
           QK GPTSERNYGLFYPNE KVY++        +   TPVN +K +V P K    G TWCV
Sbjct: 321 QKTGPTSERNYGLFYPNENKVYDVSL------NGKSTPVNDNKEKVVPVKPSLVGQTWCV 380

Query: 378 ANGNAAAEKLREGLDYACGEGGADCRAIQPGSTCYNPNSLEAHASYAFNSYYQKMGRASG 437
           ANG    EKL+EGLDYACGEGGADCR IQPG+TCYNP SLEAHASYAFNSYYQK  R  G
Sbjct: 381 ANGKTTKEKLQEGLDYACGEGGADCRPIQPGATCYNPESLEAHASYAFNSYYQKNARGVG 440

Query: 438 TCYFGGVAYVVTQPP 449
           TC FGG AYVV+QPP
Sbjct: 441 TCNFGGAAYVVSQPP 449

[Close]

BLAST of CmaCh06G008720 vs. TAIR 10
Match: AT2G05790.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 602.8 bits (1553), Expect = 2.3e-172
Identity = 297/464 (64.01%), Postives = 364/464 (78.45%), Query Frame = 0


Query: 8   FLYLLFSATAHGEPGLIGVNYGRIADNLPPPDKVVHLLKQQGIDRIKVYDTDAAVLTALS 67
           FL LL S  +  + G IGVNYGRI+D LP   KVV LLK QGI R+K++D D +VL ALS
Sbjct: 9   FLLLLLSPFSLSDAGSIGVNYGRISDELPSAFKVVQLLKSQGITRVKIFDADPSVLKALS 68

Query: 68  NSNISIVVALPNHLLSSAAADSSFTDNWVQSKISNYYPATKINAVAVGNEVFVDPNNTTN 127
            S I + V LPN LL SAA  +SF  +WV+  ++ Y+P+T+I ++AVGNEVFVD +NTT+
Sbjct: 69  GSGIKVTVDLPNELLFSAAKRTSFAVSWVKRNVAAYHPSTQIESIAVGNEVFVDTHNTTS 128

Query: 128 FLIPAMKNVYSSLQKYNLHDSIKVSSPIALTALASSYPTSSGSFKPDLIEPVIKPMLDFL 187
           FLIPAM+N++ +L  +NLH  IK+SSP+AL+AL +SYP+SSGSF+P+LI+ VIKPMLDFL
Sbjct: 129 FLIPAMRNIHKALMSFNLHSDIKISSPLALSALQNSYPSSSGSFRPELIDSVIKPMLDFL 188

Query: 188 HQTESYLMVNAYPFFAYAANSDQISLDYALFRENSGVVDPGNGLKYFSLFEAQVDAVYAA 247
            +T S LM+N YPFFAY  NSD I LDYAL REN G+VD GNGL+YF+LF+AQ+DAV+AA
Sbjct: 189 RETGSRLMINVYPFFAYEGNSDVIPLDYALLRENPGMVDSGNGLRYFNLFDAQIDAVFAA 248

Query: 248 MMALKYDDIKVVVTETGWPSIGEEKEVGASKENAASYNGNLVRRVLTGRGTPLRPEDPLD 307
           M ALKYDDI+++VTETGWPS G+E EVGA+  NAASYNGNL+RR+LT  GTPLRP+  L 
Sbjct: 249 MSALKYDDIEIIVTETGWPSKGDENEVGATLANAASYNGNLIRRILTRGGTPLRPKADLT 308

Query: 308 VFLFALFNENQKPGPTSERNYGLFYPNEEKVYEIPFT---LADMASETPTPV-NGSKAQV 367
           V+LFALFNEN+K GPTSERNYGLF+P+E+KVY+IPFT   L        TPV  G +   
Sbjct: 309 VYLFALFNENKKLGPTSERNYGLFFPDEKKVYDIPFTTEGLKHYRDGGHTPVTGGDQVTK 368

Query: 368 PPSKGD--------TWCVANGNAAAEKLREGLDYACGEGGADCRAIQPGSTCYNPNSLEA 427
           PP  G         TWCVANG+A  E+L+ GLDYACGEGGADCR IQPG+ CY+P++LEA
Sbjct: 369 PPMSGGVSKSLNGYTWCVANGDAGEERLQGGLDYACGEGGADCRPIQPGANCYSPDTLEA 428

Query: 428 HASYAFNSYYQKMGRASGTCYFGGVAYVVTQPPQYGNCEFPTGY 460
           HAS+AFNSYYQK GRA G+CYFGG AYVV+QPP+YG CEFPTGY
Sbjct: 429 HASFAFNSYYQKKGRAGGSCYFGGAAYVVSQPPKYGRCEFPTGY 472

[Close]

BLAST of CmaCh06G008720 vs. TAIR 10
Match: AT4G29360.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 379.0 bits (972), Expect = 5.3e-105
Identity = 191/453 (42.16%), Postives = 284/453 (62.69%), Query Frame = 0


Query: 19  GEPGLIGVNYGRIADNLPPPDKVVHLLKQQGIDRIKVYDTDAAVLTALSNSNISIVVALP 78
           G    IG+ YGR ADNLP P++V  L++   I  +++YD +  VL A +N+ I +++ +P
Sbjct: 22  GMASKIGICYGRNADNLPSPNRVSELIQHLNIKFVRIYDANIDVLKAFANTGIELMIGVP 81

Query: 79  NHLLSSAAADSSFTDNWVQSKISNYYPATKINAVAVGNEVFVDPNNTTNFLIPAMKNVYS 138
           N  L + A   S  D W+ + I  YYP+TKI +++VG EV   P+N T  ++PAM+N+++
Sbjct: 82  NADLLAFAQFQSNVDTWLSNNILPYYPSTKITSISVGLEVTEAPDNATGLVLPAMRNIHT 141

Query: 139 SLQKYNLHDSIKVSSPIALTALASSYPTSSGSFKPDLIEPVIKPMLDFLHQTESYLMVNA 198
           +L+K  L   IK+SS  +L  L+ S+P SS SF        +KPML+FL + ES  M++ 
Sbjct: 142 ALKKSGLDKKIKISSSHSLAILSRSFPPSSASFSKK-HSAFLKPMLEFLVENESPFMIDL 201

Query: 199 YPFFAYAANSDQISLDYALFRENSGVVDPGNGLKYFSLFEAQVDAVYAAMMALKYDDIKV 258
           YP++AY  +++++ L+YALF  +S VVDP  GL Y ++F+AQ+DA+Y A+ A+ +  +KV
Sbjct: 202 YPYYAYRDSTEKVPLEYALFESSSQVVDPATGLLYSNMFDAQLDAIYFALTAMSFKTVKV 261

Query: 259 VVTETGWPSIGEEKEVGASKENAASYNGNLVRRVLTGRGTPLRPEDPLDVFLFALFNENQ 318
           +VTE+GWPS G  KE  A+ ENA +YN NL+R V+   GTP +P + +DV+LF+LFNEN+
Sbjct: 262 MVTESGWPSKGSPKETAATPENALAYNTNLIRHVIGDPGTPAKPGEEIDVYLFSLFNENR 321

Query: 319 KPGPTSERNYGLFYPNEEKVYEIPFTLADMASETPTPVNGSKAQVPPSK----------- 378
           KPG  SERN+G+FY N   VY + FT  +    +PT      +  P S            
Sbjct: 322 KPGIESERNWGMFYANGTNVYALDFTGENTTPVSPTNSTTGTSPSPSSSPIINGNSTVTI 381

Query: 379 -----GDT--WCVANGNAAAEKLREGLDYACGEGGADCRAIQPGSTCYNPNSLEAHASYA 438
                G T  WC+A+  A+  +L+  LD+ACG G  DC A+QP   C+ P+++ +HASYA
Sbjct: 382 GGGGGGGTKKWCIASSQASVTELQTALDWACGPGNVDCSAVQPDQPCFEPDTVLSHASYA 441

Query: 439 FNSYYQKMGRASGTCYFGGVAYVVTQPPQYGNC 454
           FN+YYQ+ G +S  C F G +  V + P YGNC
Sbjct: 442 FNTYYQQSGASSIDCSFNGASVEVDKDPSYGNC 473

The following BLAST results are available for this feature:

BLAST of CmaCh06G008720 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8VYE5	7.5e-104	42.16	Glucan endo-1,3-beta-glucosidase 12 OS=Arabidopsis thaliana OX=3702 GN=At4g29360...	[more]
Q9FJU9	2.0e-101	41.55	Glucan endo-1,3-beta-glucosidase 13 OS=Arabidopsis thaliana OX=3702 GN=At5g56590...	[more]
Q9M069	7.2e-99	41.67	Glucan endo-1,3-beta-glucosidase 7 OS=Arabidopsis thaliana OX=3702 GN=At4g34480 ...	[more]
Q9ZU91	7.0e-94	40.31	Glucan endo-1,3-beta-glucosidase 3 OS=Arabidopsis thaliana OX=3702 GN=At2g01630 ...	[more]
Q94CD8	5.0e-92	39.21	Glucan endo-1,3-beta-glucosidase 4 OS=Arabidopsis thaliana OX=3702 GN=At3g13560 ...	[more]

BLAST of CmaCh06G008720 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1KYS6	4.6e-266	100.00	(1->3)-beta-glucan endohydrolase OS=Cucurbita maxima OX=3661 GN=LOC111498785 PE=...	[more]
A0A6J1GIP4	7.4e-256	96.73	(1->3)-beta-glucan endohydrolase OS=Cucurbita moschata OX=3662 GN=LOC111454517 P...	[more]
A0A6J1HJA3	2.4e-214	81.56	(1->3)-beta-glucan endohydrolase OS=Cucurbita maxima OX=3661 GN=LOC111465026 PE=...	[more]
A0A6J1EW84	6.0e-213	81.09	(1->3)-beta-glucan endohydrolase OS=Cucurbita moschata OX=3662 GN=LOC111438639 P...	[more]
A0A5D3DKE2	3.0e-196	75.05	(1->3)-beta-glucan endohydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676...	[more]

BLAST of CmaCh06G008720 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023005925.1	9.6e-266	100.00	glucan endo-1,3-beta-glucosidase 12-like [Cucurbita maxima]	[more]
KAG7028424.1	9.0e-256	96.73	Glucan endo-1,3-beta-glucosidase 13, partial [Cucurbita argyrosperma subsp. argy...	[more]
XP_022951783.1	1.5e-255	96.73	glucan endo-1,3-beta-glucosidase 12-like [Cucurbita moschata]	[more]
KAG6596945.1	3.4e-255	96.51	Glucan endo-1,3-beta-glucosidase 13, partial [Cucurbita argyrosperma subsp. soro...	[more]
XP_023520756.1	4.5e-255	96.51	glucan endo-1,3-beta-glucosidase 12-like [Cucurbita pepo subsp. pepo]	[more]

BLAST of CmaCh06G008720 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G26830.1	1.2e-184	68.11	O-Glycosyl hydrolases family 17 protein	[more]
AT5G55180.1	1.2e-184	70.63	O-Glycosyl hydrolases family 17 protein	[more]
AT5G55180.2	2.1e-178	70.57	O-Glycosyl hydrolases family 17 protein	[more]
AT2G05790.1	2.3e-172	64.01	O-Glycosyl hydrolases family 17 protein	[more]
AT4G29360.1	5.3e-105	42.16	O-Glycosyl hydrolases family 17 protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR012946	X8 domain	SMART	SM00768	X8_cls	coord: 370..455 e-value: 1.8E-48 score: 177.0
IPR012946	X8 domain	PFAM	PF07983	X8	coord: 370..441 e-value: 9.7E-23 score: 80.7
None	No IPR available	GENE3D	3.20.20.80	Glycosidases	coord: 23..350 e-value: 1.9E-127 score: 426.6
None	No IPR available	GENE3D	1.20.58.1040		coord: 367..455 e-value: 6.0E-11 score: 44.6
None	No IPR available	PANTHER	PTHR32227:SF299	O-GLYCOSYL HYDROLASES FAMILY 17 PROTEIN	coord: 9..452
IPR000490	Glycoside hydrolase family 17	PFAM	PF00332	Glyco_hydro_17	coord: 24..343 e-value: 1.4E-93 score: 313.9
IPR000490	Glycoside hydrolase family 17	PROSITE	PS00587	GLYCOSYL_HYDROL_F17	coord: 256..269
IPR044965	Glycoside hydrolase family 17, plant	PANTHER	PTHR32227	GLUCAN ENDO-1,3-BETA-GLUCOSIDASE BG1-RELATED-RELATED	coord: 9..452
IPR017853	Glycoside hydrolase superfamily	SUPERFAMILY	51445	(Trans)glycosidases	coord: 24..341

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmaCh06G008720.1	CmaCh06G008720.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0005975	carbohydrate metabolic process
cellular_component	GO:0046658	anchored component of plasma membrane
molecular_function	GO:0042973	glucan endo-1,3-beta-D-glucosidase activity
molecular_function	GO:0004553	hydrolase activity, hydrolyzing O-glycosyl compounds