CmaCh06G008300 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh06G008300
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Descriptionpoly(U)-specific endoribonuclease-B-like
LocationCma_Chr06: 4508247 .. 4512979 (-)
RNA-Seq ExpressionCmaCh06G008300
SyntenyCmaCh06G008300
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
TATTCAACCACCTCCTTCACCACTCTCAATTCACACTCTCTCTGTATCTCTGCATCTGAACATGGAGGGTTTAATCAAGGGTCTCATAGATGTCGCAATCGGCCACGACGACGACAACAACAACAGCAACGAACAATCGGCTCCCCAGTCTCGTGACGAACGATCCAGATCCACTTGGGCTCAGGTACCTCTGGGTTTTGTGATAGTGGATTTGATGTTATTTAACATGTTTTGTGATGATTTGGGCTTGGAATTGTGCAGGTAGTCACTGGGGAGGAGGATGCGGATCAAGATGGGGGAGATCGGGGACATGATCGGAGACATGGGAATGATTGGAATCGGAGGGACAATGTGGTCTGTGTTGGGCTGAGATTGATTGTGGAATTGAATGTATTTGTGGTTTTGAATTGGATTTTATGTGGAATTGAAATCTAGGGTTTTATTGATTGATTTTGCAGAAACAGGGTCGTTCGCGGCGTGAGGAGGAGTGGGAGGTTGAGGGTTCGAGGATTTCTGGTCAACGACAGCAGGTTAGATCTGTTTTCTTCACCATTCCAAGTGTTGAGATCATTTTTTTCTCATTGTTATACTTCTCTCAAGTCGCTCTAATGTTCTTTCCTTCCAATGAGATTTACACATTTTAAAAGTTTAGATTCACATTTCAGGGTCTTCTTCTTCATAGCTTAACATTGGGAAACTTTGAATTGGCTGATGTGACCCCTTAATCCTATGGGCAGTTCTCTACTAATTCTCTTTTAGCAGACATGGGTCGTATCACTTACCTCATTGAGACCTCTTTGTCTAAACCCATATAGAAGGACAACTACCCAAAGAAAATCCAAAATTTCCTTTGGGAGCTATCGCACAGAGTCATCAGTACCAAAAATGTTCTTCGAAGGCTTATGCTATTCATGGCTTTATCCCCTGGGTGGTGTTCTCTTTGTAAACCTGATGATGAAACCCAGAGTCACATCTTTATATACTGTCCATTTGCAAGGAGATTCTTCCTTTGGCTGGCATTTGGTTTGTCCTAGAAATGTTAAGGATTTTCTCATCTCAGTTCTTATTGGGGCACCCATTCAAGAATATTAAGGCTTTTCTGTGATAAATTTTACATCCCATTTGTAAATTTCATGCATCAATGAATTACATACACACATATATGTATATTATATGTAGACACATACATATATACGTGCATATTAAACATCTGTAGTAGGTATCCTGGTATGTATATCGAGTAAATTGTACAAGCTTTGTTATTTCGACCTCTTCTAAGGTCTTATCATTTGATTCTTATCTCGATATCACTCAAGTTGCTTATCCTAGAAAGAGAAATTTTCCCACAAGTGATGATAAATGCTGTAGTTGCTTTTATTAGAATTATTTTTTTTTTGACATTTTCTTTTATTAGATTCAATTATGTCGATTGATGTTTTCAGCTATTTTATCACTAGTTCAGTCCTAATAGCAGTTTCATTTCCTCCAATCTGATTGATAAAATGGCTGACTTCATATGAATCGTGTGAACAGGGTGAATATGGTAGCGAGCATGGTTACAACAGAGATCAGTGGGCTAAAAAGGTCTTTTTCTCTTGTTTTTTAATTTTCTGTGTTTTCTATTCCATGAAATTGATTGGACCCTTTTTTATCAACGTTTGGTTTTTGATTTTTAGGTTTCGAAATTGAAGCCTATAAATGGGGGTTTCCTTGTTTTGATATCTACTTTTCATATATTTTCACAAACCAAGCCATGTTTAGAAAACTAAAAAAGTAGTTTTAAAAATCTTGTTTTAGTTTATGAATTTAGCAAAGAATTCAAAATGTTTTCTTTAAGAAAGATGAAATTGATGGTAGAAAAATAATATGAAAACATGCACAATGTTCCAAATCTAAAAACCAACAACTAGAAATAAAAAATAAATTGGTTAGCAAACGAGAACTTAGTTCTTTTCACTTATATTTTCTAATGGTATTAACTGGCACAGGAGAGCTCTGAGGAGAACAATGATGGGTGGGAGACTGTTCACAAAAAGGCACCTAGGAAACATCACAAGGTTACTTATCATACGAAAAGTATTTTGACAAATACATGTTGGATGTGTTGTTTTATATGGCCAATTAGTCCATATACTTGCTTGGAAGAGAACTTGTAACAAAATTTCAGTTCTTTTATACATTACATTAAAGAGAGCCTTAACGTTATGCAAGATGTCATTGAGTCTGAATGCCTACCTTGGATGGTGTACGAACATAAAGATGAAATCATTGGTGTAGCTCTTGAAGTAAGCAATGTTTGTAAGCATGATAATCATGGGCATTTATGATGTTTACTTAGAACTAAAAGTTATTTGTGTTTATTAGTTGATAATTGGGGTGTGCAGTAGCATTAGTTAAGGTTAGCAGATCTTGAGTGTGACATATTTAGCTACAGATGCATAATTAGTCATACCTTTTCCAATCGTACTATCTTTTTTCATTTTTTTCCCTTTTTTAGAGCAAGAAACCAAACTTTCATTGAGAAAAATAATAATAATATATAAAGGCATACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCATGTTTAATCTTCAAATGGGTGTTCACCTTCGGATCATGCCGTCATCTATGTGGTGGTAGTCTGATGTATGGATGATTCAGAGGATTTGTGATGTTTGATGTTTCTATTACAAGATCATGCTTACCATGCATTTTCACTTAGTTTCTGTGCAAGTCTTTTCGATTTATGACTAGTGGTGAACTTTGAGAGGTTTCATGATAGTTGCTTGGAGTTCAAACAAACTCTAAAATTTACTCCTCTGCAATAAATTTTTAGGTGCAGGTAGAAAATTGGGAAGGATACAAACGGCCTGCCAGTGAACAAAACTATTCTGATGAGGTGGAAGTAGGTGCTGAGCTAGAACCCTCTGAGGACGAGCTTGCTGATTTTGCCCGGGCCTGTAACAAGCTATGGGAACTTGATTTAAATCGTTTGGTACCTGGACAAGATTATGAAATTGATTGTGGTGAAGGAAAAAGGGTCCATGGAAGGGAAGATATGGCACAAGCAAGCCTCTTTAGTTGGGTTAATGAAGACGTATTTAGGAAGCCAACATTTTCACGTTTTTGCTCTCTACTTGATAACTACAATCCCGATCAAGGGAGCAAAGAAGTTGTGACACCTGAGGAGAGGCAAGAGCAGGCTGCATTCATAGAAGAAATTAGTAGAACTGCACCAATTAAGTACCTTCATAAGTACCTTTTATACAAGGGAGTTGCATCAAATGACTATCAAGATTTCAAACGTATGCTGACCAGTCTGTGGTTCGATCTTTATGGTCGGGGTGGAACATCTCATTCCTCTTCTGCCTTTGAACATGTTTTTGTTGGAGAAATCAAGCAACAAGGTGGACAAGAGGTTTCTGGTTTCCACAACTGGCTTCAGGTTAGTGCCTTCCTGAGATTAGCTATCACAAAAGATAATGCTTGAAATTTAAGATATAAATAATCGATCTGTTTAATATTGTATCTAACCGTTGAGGCTCTTCATGTATTCACCAAGGTTAATATTAAGCAAGGGGGCGGAACAAAGAAGGAAAATGTTGCCCTCTTGGCTTGTCTTAGGTTAAAAAACTATATAAAAAGGTAAAAATTTTAAGGTTTTCTCTCTAATTTGCTAATTTCCAAATTATATGTATAATAAGAAAAGTTAGAAAGTAAAATCTTTCTACATCGTTTGTAAACTCTGGATTCTCCCCTTAAATTTCTCCCCTTAAATGGAAATGAACAACTTGGATCAGTTCATCTTAGAATACATTTTTAAATTGTGAAGTTGACTGAGTTCTTGTCCGTAAATGAATATAACGTTTTGTCCCCAAATCTAACGAACCTATCATTTTCTTAATATTAGTATTTTCTTTGCTGGAGTTTCCCGAACTTATTTTCTTCCTTTATGTCTGACTTTTCTTCCATGTGCATTTATTCCAAATCTAGTTTTACCTTGAAGAAGCTAAAGGGAGTGTTGACTATCAAGGTTATATTTTCCCCAGACGACGTGGCCAGTTTGTAAGTAACTCAACTTCACGTTTTTTTCTTTTGGTGGTATTTGATGTTGAGATGTAAAGAAATAAGTTGTGAATGCCCATGCCATGCAGCATCATTCAGGCTATTAAAAGAAACTAAATGATCATTTATGATTCAGGCCTTTTGTGGTTACCATACAAGAATAACCTTCATTTTCCTGACATTCTTCAGACGATTAAATTTGTGTTATTCCACCAAACTTTAGTTTTCATTAGATCAGCCTTTCCCACTTTAAATTCTAGAGCTTGCCTCACTTTTTGGAAGATAAGCATTCTCTTCTCTGTAATCTAAACCATGTCTCTTACTTAACTATTTGTTTGCTAACCCTGCAGCCTGATTCAGAAACTCAATTGCTTACCATTCAGTTTGAATGGAATGGTGTTCTCAAATCTGTCTCAAGCACTTTAGTAGGAGTTAGCCCTGAGTTTGAACTTGCACTGTATACACTATGCTTCTTCCTTGGCGGAGAGGATAACCATGTTGAGCTCGGACCATATCCTGTTAATATCAAGTGCTATCGATTAGGAAACAAAATCGGGTCTGTGTTTCCTGTAGCAGAGTCTTGA

mRNA sequence

TATTCAACCACCTCCTTCACCACTCTCAATTCACACTCTCTCTGTATCTCTGCATCTGAACATGGAGGGTTTAATCAAGGGTCTCATAGATGTCGCAATCGGCCACGACGACGACAACAACAACAGCAACGAACAATCGGCTCCCCAGTCTCGTGACGAACGATCCAGATCCACTTGGGCTCAGGTAGTCACTGGGGAGGAGGATGCGGATCAAGATGGGGGAGATCGGGGACATGATCGGAGACATGGGAATGATTGGAATCGGAGGGACAATGTGAAACAGGGTCGTTCGCGGCGTGAGGAGGAGTGGGAGGTTGAGGGTTCGAGGATTTCTGGTCAACGACAGCAGGGTGAATATGGTAGCGAGCATGGTTACAACAGAGATCAGTGGGCTAAAAAGGAGAGCTCTGAGGAGAACAATGATGGGTGGGAGACTGTTCACAAAAAGGCACCTAGGAAACATCACAAGGTGCAGGTAGAAAATTGGGAAGGATACAAACGGCCTGCCAGTGAACAAAACTATTCTGATGAGGTGGAAGTAGGTGCTGAGCTAGAACCCTCTGAGGACGAGCTTGCTGATTTTGCCCGGGCCTGTAACAAGCTATGGGAACTTGATTTAAATCGTTTGGTACCTGGACAAGATTATGAAATTGATTGTGGTGAAGGAAAAAGGGTCCATGGAAGGGAAGATATGGCACAAGCAAGCCTCTTTAGTTGGGTTAATGAAGACGTATTTAGGAAGCCAACATTTTCACGTTTTTGCTCTCTACTTGATAACTACAATCCCGATCAAGGGAGCAAAGAAGTTGTGACACCTGAGGAGAGGCAAGAGCAGGCTGCATTCATAGAAGAAATTAGTAGAACTGCACCAATTAAGTACCTTCATAAGTACCTTTTATACAAGGGAGTTGCATCAAATGACTATCAAGATTTCAAACGTATGCTGACCAGTCTGTGGTTCGATCTTTATGGTCGGGGTGGAACATCTCATTCCTCTTCTGCCTTTGAACATGTTTTTGTTGGAGAAATCAAGCAACAAGGTGGACAAGAGGTTTCTGGTTTCCACAACTGGCTTCAGTTTTACCTTGAAGAAGCTAAAGGGAGTGTTGACTATCAAGGTTATATTTTCCCCAGACGACGTGGCCAGTTTCCTGATTCAGAAACTCAATTGCTTACCATTCAGTTTGAATGGAATGGTGTTCTCAAATCTGTCTCAAGCACTTTAGTAGGAGTTAGCCCTGAGTTTGAACTTGCACTGTATACACTATGCTTCTTCCTTGGCGGAGAGGATAACCATGTTGAGCTCGGACCATATCCTGTTAATATCAAGTGCTATCGATTAGGAAACAAAATCGGGTCTGTGTTTCCTGTAGCAGAGTCTTGA

Coding sequence (CDS)

ATGGAGGGTTTAATCAAGGGTCTCATAGATGTCGCAATCGGCCACGACGACGACAACAACAACAGCAACGAACAATCGGCTCCCCAGTCTCGTGACGAACGATCCAGATCCACTTGGGCTCAGGTAGTCACTGGGGAGGAGGATGCGGATCAAGATGGGGGAGATCGGGGACATGATCGGAGACATGGGAATGATTGGAATCGGAGGGACAATGTGAAACAGGGTCGTTCGCGGCGTGAGGAGGAGTGGGAGGTTGAGGGTTCGAGGATTTCTGGTCAACGACAGCAGGGTGAATATGGTAGCGAGCATGGTTACAACAGAGATCAGTGGGCTAAAAAGGAGAGCTCTGAGGAGAACAATGATGGGTGGGAGACTGTTCACAAAAAGGCACCTAGGAAACATCACAAGGTGCAGGTAGAAAATTGGGAAGGATACAAACGGCCTGCCAGTGAACAAAACTATTCTGATGAGGTGGAAGTAGGTGCTGAGCTAGAACCCTCTGAGGACGAGCTTGCTGATTTTGCCCGGGCCTGTAACAAGCTATGGGAACTTGATTTAAATCGTTTGGTACCTGGACAAGATTATGAAATTGATTGTGGTGAAGGAAAAAGGGTCCATGGAAGGGAAGATATGGCACAAGCAAGCCTCTTTAGTTGGGTTAATGAAGACGTATTTAGGAAGCCAACATTTTCACGTTTTTGCTCTCTACTTGATAACTACAATCCCGATCAAGGGAGCAAAGAAGTTGTGACACCTGAGGAGAGGCAAGAGCAGGCTGCATTCATAGAAGAAATTAGTAGAACTGCACCAATTAAGTACCTTCATAAGTACCTTTTATACAAGGGAGTTGCATCAAATGACTATCAAGATTTCAAACGTATGCTGACCAGTCTGTGGTTCGATCTTTATGGTCGGGGTGGAACATCTCATTCCTCTTCTGCCTTTGAACATGTTTTTGTTGGAGAAATCAAGCAACAAGGTGGACAAGAGGTTTCTGGTTTCCACAACTGGCTTCAGTTTTACCTTGAAGAAGCTAAAGGGAGTGTTGACTATCAAGGTTATATTTTCCCCAGACGACGTGGCCAGTTTCCTGATTCAGAAACTCAATTGCTTACCATTCAGTTTGAATGGAATGGTGTTCTCAAATCTGTCTCAAGCACTTTAGTAGGAGTTAGCCCTGAGTTTGAACTTGCACTGTATACACTATGCTTCTTCCTTGGCGGAGAGGATAACCATGTTGAGCTCGGACCATATCCTGTTAATATCAAGTGCTATCGATTAGGAAACAAAATCGGGTCTGTGTTTCCTGTAGCAGAGTCTTGA

Protein sequence

MEGLIKGLIDVAIGHDDDNNNSNEQSAPQSRDERSRSTWAQVVTGEEDADQDGGDRGHDRRHGNDWNRRDNVKQGRSRREEEWEVEGSRISGQRQQGEYGSEHGYNRDQWAKKESSEENNDGWETVHKKAPRKHHKVQVENWEGYKRPASEQNYSDEVEVGAELEPSEDELADFARACNKLWELDLNRLVPGQDYEIDCGEGKRVHGREDMAQASLFSWVNEDVFRKPTFSRFCSLLDNYNPDQGSKEVVTPEERQEQAAFIEEISRTAPIKYLHKYLLYKGVASNDYQDFKRMLTSLWFDLYGRGGTSHSSSAFEHVFVGEIKQQGGQEVSGFHNWLQFYLEEAKGSVDYQGYIFPRRRGQFPDSETQLLTIQFEWNGVLKSVSSTLVGVSPEFELALYTLCFFLGGEDNHVELGPYPVNIKCYRLGNKIGSVFPVAES
Homology
BLAST of CmaCh06G008300 vs. ExPASy Swiss-Prot
Match: Q503V9 (Poly(U)-specific endoribonuclease-B OS=Danio rerio OX=7955 GN=endoub PE=2 SV=1)

HSP 1 Score: 224.6 bits (571), Expect = 2.3e-57
Identity = 119/265 (44.91%), Postives = 154/265 (58.11%), Query Frame = 0

Query: 180 KLWELDLNRLVPGQDYEID----CGEGKRVHGREDMAQASLFSWVNEDVFRKPTFSRFCS 239
           +LW+ D NRL PG DY I      G+   V    D A   LFS+V+E++F+K TF  F S
Sbjct: 14  ELWDNDTNRLRPGIDYRISLQGKAGDLAGVPDDSDGAGMPLFSFVDENIFKKETFLAFIS 73

Query: 240 LLDNYNPDQGSKEVVTPEERQEQAAFIEEISRTAPIKYLHKYLLYKGVASNDYQDFKRML 299
           LLDNY  D G  E+VTPEE  E   F++ + +T  +K  HKYL+ K ++  D   FK+ L
Sbjct: 74  LLDNYESDTGEPEIVTPEEEMENHRFLDSVIKTPTMKIAHKYLVEKRLSPEDTTGFKQQL 133

Query: 300 TSLWFDLYGRGGTSH-SSSAFEHVFVGEIKQQGGQEVSGFHNWLQFYLEEAKGSVDYQGY 359
             +WF+LY R G+S   SS FEHVFVGE +  GG  V GFHNW+Q YL+E  G +DY+GY
Sbjct: 134 YKIWFELYARKGSSKPDSSGFEHVFVGETR--GGHTVIGFHNWIQLYLQEKLGHIDYKGY 193

Query: 360 IFPRRRGQFPDSETQLLTIQFEWNGVLKSVSSTLVGVSPEFELALYTLCFFLG-GEDNHV 419
                  Q PD    +L +QF W   +K   S  +GVSPEFE +LYTLCF +   E   V
Sbjct: 194 SVNANSPQ-PDENKHMLALQFSWKNGIKPKGSIFIGVSPEFEFSLYTLCFLMSPNERVKV 253

Query: 420 ELGPYPVNIKCYRLGNK-IGSVFPV 438
               Y V I C+    K IG+ +PV
Sbjct: 254 SFNLYDVEIVCHHYNQKHIGTTYPV 275

BLAST of CmaCh06G008300 vs. ExPASy Swiss-Prot
Match: B1WBB4 (Poly(U)-specific endoribonuclease-B OS=Xenopus laevis OX=8355 GN=endou-b PE=2 SV=1)

HSP 1 Score: 210.3 bits (534), Expect = 4.4e-53
Identity = 115/275 (41.82%), Postives = 160/275 (58.18%), Query Frame = 0

Query: 173 DFARACNKLWELDLNRLVPGQDYEIDCGEGK------RVHGREDMAQASLFSWVNEDVFR 232
           + ++  N+LW+ D+NR+  G+DY I   +GK      R +  +D A   LF +V+E+  +
Sbjct: 11  ELSKLFNELWDADVNRMKAGKDYRISL-QGKAGYVPARSNQAKDSASYPLFQFVDEEKLK 70

Query: 233 -KPTFSRFCSLLDNYNPDQGSKEVVTPEERQEQAAFIEEISRTAPIKYLHKYLLYKGVAS 292
            K TF+ F SLLDNY  D G  EVVTPEE  E   F++ I  T  +K  H YL+ K  A 
Sbjct: 71  SKKTFATFISLLDNYEMDTGVAEVVTPEEIAENNNFLDAILETKVMKMAHDYLVRKNQAK 130

Query: 293 NDYQDFKRMLTSLWFDLYGRG-GTSHSSSAFEHVFVGEIKQQGGQEVSGFHNWLQFYLEE 352
               DFK  L S+WF LY R  G+   S  FEHVFVGE K+  G+E+ G HNW+QFYL+E
Sbjct: 131 PSQNDFKVQLYSIWFQLYSRAPGSRPDSCGFEHVFVGESKR--GKEMMGLHNWVQFYLQE 190

Query: 353 AKGSVDYQGYIFPRRRGQFPDSETQLLTIQFEWNGVLKSVSSTLVGVSPEFELALYTLCF 412
            + ++DY+GY+  R+    PD + Q+L +QF W  ++K V S+ +GVSPEFE ALYT+ F
Sbjct: 191 KRKNIDYKGYV-ARQNKSRPDEDDQVLNLQFSWKEMVKPVGSSFIGVSPEFEFALYTIVF 250

Query: 413 FLGG---EDNHVELGPYPVNIKCYRLGNKIGSVFP 437
                      + L  Y + I   R G  IG+ +P
Sbjct: 251 LASQAKMSREVIRLEEYELQIVVNRHGRYIGTAYP 281

BLAST of CmaCh06G008300 vs. ExPASy Swiss-Prot
Match: Q8JFY9 (Poly(U)-specific endoribonuclease-A OS=Xenopus laevis OX=8355 GN=endou-a PE=1 SV=1)

HSP 1 Score: 209.1 bits (531), Expect = 9.8e-53
Identity = 116/276 (42.03%), Postives = 159/276 (57.61%), Query Frame = 0

Query: 173 DFARACNKLWELDLNRLVPGQDYEIDCGEGKR------VHGREDMAQASLFSWVNEDVFR 232
           + ++  N+LW+ D NR+  G+DY I   +GK        +   D A   LF +V+E+  +
Sbjct: 11  ELSKLFNELWDADQNRMKSGKDYRISL-QGKAGYVPAGSNQARDSASFPLFQFVDEEKLK 70

Query: 233 -KPTFSRFCSLLDNYNPDQGSKEVVTPEERQEQAAFIEEISRTAPIKYLHKYLLYKGVAS 292
            + TF+ F SLLDNY  D G  EVVTPEE  E   F++ I  T  +K  H YL+ K  A 
Sbjct: 71  SRKTFATFISLLDNYEMDTGVAEVVTPEEIAENNNFLDAILETKVMKMAHDYLVRKNQAK 130

Query: 293 NDYQDFKRMLTSLWFDLYGRG-GTSHSSSAFEHVFVGEIKQQGGQEVSGFHNWLQFYLEE 352
               DFK  L ++WF LY R  G+   S  FEHVFVGE K+  GQE+ G HNW+QFYL+E
Sbjct: 131 PTRNDFKVQLYNIWFQLYSRAPGSRPDSCGFEHVFVGESKR--GQEMMGLHNWVQFYLQE 190

Query: 353 AKGSVDYQGYIFPRRRGQFPDSETQLLTIQFEWNGVLKSVSSTLVGVSPEFELALYTLCF 412
            + ++DY+GY+  R+    PD + Q+L +QF W  ++K V S+ +GVSPEFE ALYT+ F
Sbjct: 191 KRKNIDYKGYV-ARQNKSRPDEDDQVLNLQFNWKEMVKPVGSSFIGVSPEFEFALYTIVF 250

Query: 413 FLGGED---NHVELGPYPVNIKCYRLGNKIGSVFPV 438
               E      V L  Y + I   R G  IG+ +PV
Sbjct: 251 LASQEKMSREVVRLEEYELQIVVNRHGRYIGTAYPV 282

BLAST of CmaCh06G008300 vs. ExPASy Swiss-Prot
Match: B1H3D5 (Poly(U)-specific endoribonuclease OS=Xenopus tropicalis OX=8364 GN=endou PE=2 SV=1)

HSP 1 Score: 208.0 bits (528), Expect = 2.2e-52
Identity = 114/276 (41.30%), Postives = 158/276 (57.25%), Query Frame = 0

Query: 173 DFARACNKLWELDLNRLVPGQDYEIDCGEGKR------VHGREDMAQASLFSWVNEDVFR 232
           + ++  N+LW+ D NR+  G+DY I   +GK        +   D A   LF +V+E+  R
Sbjct: 12  ELSKLFNELWDADENRMKAGKDYRISL-QGKAGYVPAGSNQARDSASYPLFQFVDEEKLR 71

Query: 233 -KPTFSRFCSLLDNYNPDQGSKEVVTPEERQEQAAFIEEISRTAPIKYLHKYLLYKGVAS 292
            + TF+ F SLLDNY  D G  EVVTPEE  E   F++ I  T  +K  H YL+ K  A 
Sbjct: 72  SRKTFATFISLLDNYEMDTGVAEVVTPEEIAENNTFLDAILETKVMKIAHDYLVRKNQAK 131

Query: 293 NDYQDFKRMLTSLWFDLYGRG-GTSHSSSAFEHVFVGEIKQQGGQEVSGFHNWLQFYLEE 352
               DFK  L ++WF LY R  G    S  FEHVFVGE K+  G+E+ G HNW+QFYL+E
Sbjct: 132 PSRNDFKAQLYTIWFQLYSRAPGQGPDSCGFEHVFVGESKR--GKEIMGLHNWVQFYLQE 191

Query: 353 AKGSVDYQGYIFPRRRGQFPDSETQLLTIQFEWNGVLKSVSSTLVGVSPEFELALYTLCF 412
            + ++DY+GY+  R+    PD + Q+L +QF W  ++K + S+ +GVSPEFE ALYT+ F
Sbjct: 192 KRKTIDYKGYV-GRQNKSRPDHDDQVLNLQFSWKEMVKPIGSSFIGVSPEFEFALYTVVF 251

Query: 413 FLGGED---NHVELGPYPVNIKCYRLGNKIGSVFPV 438
               E      V +  Y + I   R G  IG+ +PV
Sbjct: 252 LTSQEKMTREVVRVEEYEMQIVVNRHGRYIGTAYPV 283

BLAST of CmaCh06G008300 vs. ExPASy Swiss-Prot
Match: Q1LUM3 (Poly(U)-specific endoribonuclease-C OS=Danio rerio OX=7955 GN=endouc PE=2 SV=1)

HSP 1 Score: 191.8 bits (486), Expect = 1.6e-47
Identity = 111/271 (40.96%), Postives = 157/271 (57.93%), Query Frame = 0

Query: 179 NKLWELDLNRLVPGQDYEIDCGEGKRVHGRE------DMAQASLFSWVNEDVFRK-PTFS 238
           N+LW+LD+NR+ P  +Y I   +GK  +  +      D A + LF  V+E       T++
Sbjct: 36  NELWKLDVNRMEPLTNYNISL-QGKAGYIPQGSTNVVDHASSPLFVNVDEAKLSSITTYA 95

Query: 239 RFCSLLDNYNPDQGSKEVVTPEERQEQAAFIEEISRTAPIKYLHKYLLYKGVASNDYQDF 298
           RF  LLDNY    G  E VT EE  E  +F++ I  TA +K  H+YL+ KG + +D + F
Sbjct: 96  RFMKLLDNYERSTGVAERVTAEEVTENNSFLDAILETAVMKRAHQYLIGKGKSRSDLRQF 155

Query: 299 KRMLTSLWFDLYGR---GGTSHSSSAFEHVFVGEIKQQGGQEVSGFHNWLQFYLEEAKGS 358
           K  L  +WF LY R   GG    SS FEHVFVGE K   G+E+ G HNW+QFYL+E +  
Sbjct: 156 KSQLYYMWFRLYHRERNGG--EDSSGFEHVFVGETK--FGREIMGLHNWVQFYLQEKQNL 215

Query: 359 VDYQGYIFPRRRGQFPDSETQLLTIQFEWNGVLKSVSSTLVGVSPEFELALYTLCFFLGG 418
           +DY+GY    R    PD++  +L +QF W+G++K V+S  VGVSPEFE+A++T+ F    
Sbjct: 216 LDYKGY--KARANDVPDADDHVLNVQFSWHGLVKPVASAFVGVSPEFEMAVFTILFLTST 275

Query: 419 EDNH---VELGPYPVNIKCYRLGNKIGSVFP 437
           E      V L  Y + +  +R G  IG+ +P
Sbjct: 276 EKTTTAVVNLDEYQLEMVVHRHGRCIGTAYP 299

BLAST of CmaCh06G008300 vs. ExPASy TrEMBL
Match: A0A6J1L240 (poly(U)-specific endoribonuclease-B-like OS=Cucurbita maxima OX=3661 GN=LOC111498407 PE=3 SV=1)

HSP 1 Score: 899.0 bits (2322), Expect = 7.6e-258
Identity = 440/440 (100.00%), Postives = 440/440 (100.00%), Query Frame = 0

Query: 1   MEGLIKGLIDVAIGHDDDNNNSNEQSAPQSRDERSRSTWAQVVTGEEDADQDGGDRGHDR 60
           MEGLIKGLIDVAIGHDDDNNNSNEQSAPQSRDERSRSTWAQVVTGEEDADQDGGDRGHDR
Sbjct: 1   MEGLIKGLIDVAIGHDDDNNNSNEQSAPQSRDERSRSTWAQVVTGEEDADQDGGDRGHDR 60

Query: 61  RHGNDWNRRDNVKQGRSRREEEWEVEGSRISGQRQQGEYGSEHGYNRDQWAKKESSEENN 120
           RHGNDWNRRDNVKQGRSRREEEWEVEGSRISGQRQQGEYGSEHGYNRDQWAKKESSEENN
Sbjct: 61  RHGNDWNRRDNVKQGRSRREEEWEVEGSRISGQRQQGEYGSEHGYNRDQWAKKESSEENN 120

Query: 121 DGWETVHKKAPRKHHKVQVENWEGYKRPASEQNYSDEVEVGAELEPSEDELADFARACNK 180
           DGWETVHKKAPRKHHKVQVENWEGYKRPASEQNYSDEVEVGAELEPSEDELADFARACNK
Sbjct: 121 DGWETVHKKAPRKHHKVQVENWEGYKRPASEQNYSDEVEVGAELEPSEDELADFARACNK 180

Query: 181 LWELDLNRLVPGQDYEIDCGEGKRVHGREDMAQASLFSWVNEDVFRKPTFSRFCSLLDNY 240
           LWELDLNRLVPGQDYEIDCGEGKRVHGREDMAQASLFSWVNEDVFRKPTFSRFCSLLDNY
Sbjct: 181 LWELDLNRLVPGQDYEIDCGEGKRVHGREDMAQASLFSWVNEDVFRKPTFSRFCSLLDNY 240

Query: 241 NPDQGSKEVVTPEERQEQAAFIEEISRTAPIKYLHKYLLYKGVASNDYQDFKRMLTSLWF 300
           NPDQGSKEVVTPEERQEQAAFIEEISRTAPIKYLHKYLLYKGVASNDYQDFKRMLTSLWF
Sbjct: 241 NPDQGSKEVVTPEERQEQAAFIEEISRTAPIKYLHKYLLYKGVASNDYQDFKRMLTSLWF 300

Query: 301 DLYGRGGTSHSSSAFEHVFVGEIKQQGGQEVSGFHNWLQFYLEEAKGSVDYQGYIFPRRR 360
           DLYGRGGTSHSSSAFEHVFVGEIKQQGGQEVSGFHNWLQFYLEEAKGSVDYQGYIFPRRR
Sbjct: 301 DLYGRGGTSHSSSAFEHVFVGEIKQQGGQEVSGFHNWLQFYLEEAKGSVDYQGYIFPRRR 360

Query: 361 GQFPDSETQLLTIQFEWNGVLKSVSSTLVGVSPEFELALYTLCFFLGGEDNHVELGPYPV 420
           GQFPDSETQLLTIQFEWNGVLKSVSSTLVGVSPEFELALYTLCFFLGGEDNHVELGPYPV
Sbjct: 361 GQFPDSETQLLTIQFEWNGVLKSVSSTLVGVSPEFELALYTLCFFLGGEDNHVELGPYPV 420

Query: 421 NIKCYRLGNKIGSVFPVAES 441
           NIKCYRLGNKIGSVFPVAES
Sbjct: 421 NIKCYRLGNKIGSVFPVAES 440

BLAST of CmaCh06G008300 vs. ExPASy TrEMBL
Match: A0A6J1F7L9 (poly(U)-specific endoribonuclease-B-like OS=Cucurbita moschata OX=3662 GN=LOC111443088 PE=3 SV=1)

HSP 1 Score: 864.4 bits (2232), Expect = 2.1e-247
Identity = 426/440 (96.82%), Postives = 432/440 (98.18%), Query Frame = 0

Query: 1   MEGLIKGLIDVAIGHDDDNNNSNEQSAPQSRDERSRSTWAQVVTGEEDADQDGGDRGHDR 60
           MEGLIKGLIDVAIGHDDD N  NEQSA QSRDERSRSTWAQVVTGEEDAD+DGGDRGHDR
Sbjct: 1   MEGLIKGLIDVAIGHDDDKN--NEQSASQSRDERSRSTWAQVVTGEEDADRDGGDRGHDR 60

Query: 61  RHGNDWNRRDNVKQGRSRREEEWEVEGSRISGQRQQGEYGSEHGYNRDQWAKKESSEENN 120
           RHGNDWNRRDN K+GRSRR EEWEV+GSRISGQ+QQGEYGSEHGYNRDQWAKKESSEENN
Sbjct: 61  RHGNDWNRRDNEKEGRSRR-EEWEVQGSRISGQQQQGEYGSEHGYNRDQWAKKESSEENN 120

Query: 121 DGWETVHKKAPRKHHKVQVENWEGYKRPASEQNYSDEVEVGAELEPSEDELADFARACNK 180
           DGWETVHKKAPRKHHKVQVENWEGYKRPASEQNYSDEVEVGAELEPSEDELADFARACNK
Sbjct: 121 DGWETVHKKAPRKHHKVQVENWEGYKRPASEQNYSDEVEVGAELEPSEDELADFARACNK 180

Query: 181 LWELDLNRLVPGQDYEIDCGEGKRVHGREDMAQASLFSWVNEDVFRKPTFSRFCSLLDNY 240
           LWELDLNRLVPGQDYEIDCGEGKRVHGREDMAQASLFSWV+EDVFRKPTFSRFCSLLDNY
Sbjct: 181 LWELDLNRLVPGQDYEIDCGEGKRVHGREDMAQASLFSWVSEDVFRKPTFSRFCSLLDNY 240

Query: 241 NPDQGSKEVVTPEERQEQAAFIEEISRTAPIKYLHKYLLYKGVASNDYQDFKRMLTSLWF 300
           NPDQGSKEVVTPEE+QEQAAFIEEISRTAPIKYLHKYL YKGV SNDYQDFKRMLTSLWF
Sbjct: 241 NPDQGSKEVVTPEEKQEQAAFIEEISRTAPIKYLHKYLSYKGVTSNDYQDFKRMLTSLWF 300

Query: 301 DLYGRGGTSHSSSAFEHVFVGEIKQQGGQEVSGFHNWLQFYLEEAKGSVDYQGYIFPRRR 360
           DLYGRGGTSHSSSAFEHVFVGEIKQQGGQEVSGFHNWLQFYLEEAKGSVDYQGYIFPRRR
Sbjct: 301 DLYGRGGTSHSSSAFEHVFVGEIKQQGGQEVSGFHNWLQFYLEEAKGSVDYQGYIFPRRR 360

Query: 361 GQFPDSETQLLTIQFEWNGVLKSVSSTLVGVSPEFELALYTLCFFLGGEDNHVELGPYPV 420
           GQFPDSETQLLTIQFEWNGVLKSVSSTLVGVSPEFELALYTLCFFLGGEDNHVELGPYPV
Sbjct: 361 GQFPDSETQLLTIQFEWNGVLKSVSSTLVGVSPEFELALYTLCFFLGGEDNHVELGPYPV 420

Query: 421 NIKCYRLGNKIGSVFPVAES 441
           NIKCYRLGNKIGSVFPVAES
Sbjct: 421 NIKCYRLGNKIGSVFPVAES 437

BLAST of CmaCh06G008300 vs. ExPASy TrEMBL
Match: A0A6J1DMN3 (poly(U)-specific endoribonuclease-B-like OS=Momordica charantia OX=3673 GN=LOC111022649 PE=3 SV=1)

HSP 1 Score: 748.8 bits (1932), Expect = 1.3e-212
Identity = 369/440 (83.86%), Postives = 399/440 (90.68%), Query Frame = 0

Query: 1   MEGLIKGLIDVAIGHDDDNNNSNEQSAPQSRDERSRSTWAQVVTGEEDADQDGGDRGHDR 60
           MEGLIKG++D+A+GHDD   N +E+SAPQSRDERSRSTWAQVV+GEEDA         DR
Sbjct: 1   MEGLIKGVLDIALGHDD---NRDEESAPQSRDERSRSTWAQVVSGEEDAG--------DR 60

Query: 61  RHGNDWNRRDNVKQGRSRREEEWEVEGSRISGQRQQGEYGSEHGYNRDQWAKKESSEENN 120
           R G   NRRD+ ++GRSRR +EWEVEGSRISGQRQQGEY SEHG+NR+ WA +E SEE N
Sbjct: 61  RQGYAPNRRDDEEEGRSRR-QEWEVEGSRISGQRQQGEYRSEHGHNRNHWATEEGSEEKN 120

Query: 121 DGWETVHKKAPRKHHKVQVENWEGYKRPASEQNYSDEVEVGAELEPSEDELADFARACNK 180
           DGWETVHKK PR+ HKVQ++NW GYKRP SEQNYSDEVEVGAELEPSEDELAD +RACNK
Sbjct: 121 DGWETVHKKPPRRKHKVQIDNWGGYKRPPSEQNYSDEVEVGAELEPSEDELADLSRACNK 180

Query: 181 LWELDLNRLVPGQDYEIDCGEGKRVHGREDMAQASLFSWVNEDVFRKPTFSRFCSLLDNY 240
           LWELDLNRLVPG+DYEIDCGEGKRV GREDMA+  LFSWV+ED FRKPTFSRFCSLLDNY
Sbjct: 181 LWELDLNRLVPGKDYEIDCGEGKRVSGREDMAEGCLFSWVSEDAFRKPTFSRFCSLLDNY 240

Query: 241 NPDQGSKEVVTPEERQEQAAFIEEISRTAPIKYLHKYLLYKGVASNDYQDFKRMLTSLWF 300
           NPDQGS+E+VT EERQEQAAF+EEISRTAPIKYLHKYL   GV  NDYQDFKR+LTSLWF
Sbjct: 241 NPDQGSREIVTAEERQEQAAFVEEISRTAPIKYLHKYLSSNGVTPNDYQDFKRLLTSLWF 300

Query: 301 DLYGRGGTSHSSSAFEHVFVGEIKQQGGQEVSGFHNWLQFYLEEAKGSVDYQGYIFPRRR 360
           DLYGRGGTS SSSAFEHVFVGEIKQ+G QEVSGFHNWLQFYLEEAKG+VDYQGYIFPRRR
Sbjct: 301 DLYGRGGTSGSSSAFEHVFVGEIKQRGQQEVSGFHNWLQFYLEEAKGAVDYQGYIFPRRR 360

Query: 361 GQFPDSETQLLTIQFEWNGVLKSVSSTLVGVSPEFELALYTLCFFLGGEDNHVELGPYPV 420
           GQFPDSETQLLTIQFEWNG+LKSVSSTL+GVSPEFELALYTLCFFLGGEDNHVELGPYP+
Sbjct: 361 GQFPDSETQLLTIQFEWNGILKSVSSTLIGVSPEFELALYTLCFFLGGEDNHVELGPYPI 420

Query: 421 NIKCYRLGNKIGSVFPVAES 441
           NIKCYRLGNKIGSVFPVAES
Sbjct: 421 NIKCYRLGNKIGSVFPVAES 428

BLAST of CmaCh06G008300 vs. ExPASy TrEMBL
Match: A0A0A0L6P1 (XendoU domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G017000 PE=3 SV=1)

HSP 1 Score: 743.4 bits (1918), Expect = 5.3e-211
Identity = 375/439 (85.42%), Postives = 398/439 (90.66%), Query Frame = 0

Query: 1   MEGLIKGLIDVAIGHDDDNNNSNEQSAPQSRDERSRSTWAQVVTGEEDADQDGGDRGHDR 60
           MEGLIKGLIDVAIG+D DNN  ++QS  QSR++RSRSTWAQVVTGEED        G DR
Sbjct: 1   MEGLIKGLIDVAIGNDGDNN--DQQSPSQSREDRSRSTWAQVVTGEED--------GGDR 60

Query: 61  RHGNDWNRRDNVKQGRSRREEEWEVEGSRISGQRQQGEYGSEHGYNRDQWAKKESSEENN 120
           R G   NRR+N ++GRSRR EEWEVE SRISGQR+Q EYGSEHGYNR+QWAK+ESSEE N
Sbjct: 61  RDGYHRNRRNNEEEGRSRR-EEWEVEDSRISGQRKQSEYGSEHGYNRNQWAKEESSEEKN 120

Query: 121 DGWETVHKKAPRKHHKVQVENWEGYKRPASEQNYSDEVEVGAELEPSEDELADFARACNK 180
           DGWETVHKK  R+ HKVQ +NW GYKRPASEQNYSDEVEVGAELEPSEDELAD + ACNK
Sbjct: 121 DGWETVHKKPTRRQHKVQTDNWGGYKRPASEQNYSDEVEVGAELEPSEDELADLSYACNK 180

Query: 181 LWELDLNRLVPGQDYEIDCGEGKRVHGREDMAQASLFSWVNEDVFRKPTFSRFCSLLDNY 240
           LW+LDLNRLVPG+DYEIDCGEGKRV GREDMAQ SLFSWV+E+V RKPTFSRFCSLLDNY
Sbjct: 181 LWKLDLNRLVPGKDYEIDCGEGKRVSGREDMAQGSLFSWVSEEVLRKPTFSRFCSLLDNY 240

Query: 241 NPDQGSKEVVTPEERQEQAAFIEEISRTAPIKYLHKYLLYKGVASNDYQDFKRMLTSLWF 300
           NP+QGSKEVVTPEERQEQAAFIEEISRTAPIKYLHKYL  KGVASN+YQDFKRMLTSLWF
Sbjct: 241 NPNQGSKEVVTPEERQEQAAFIEEISRTAPIKYLHKYLSSKGVASNEYQDFKRMLTSLWF 300

Query: 301 DLYGRGGTSHSSSAFEHVFVGEIKQQGGQEVSGFHNWLQFYLEEAKGSVDYQGYIFPRRR 360
           DLYGRGGT  SSSAFEHVFVGEIKQQG QEVSGFHNWLQFYLEEAKGSVDYQGYIFPRRR
Sbjct: 301 DLYGRGGTCGSSSAFEHVFVGEIKQQGEQEVSGFHNWLQFYLEEAKGSVDYQGYIFPRRR 360

Query: 361 GQFPDSETQLLTIQFEWNGVLKSVSSTLVGVSPEFELALYTLCFFLGGEDNHVELGPYPV 420
           GQ PDSETQLLTIQFEWNGVLKSVSSTLVGVSPEFE+ALYTLCFFLGGEDNHVELGPY V
Sbjct: 361 GQCPDSETQLLTIQFEWNGVLKSVSSTLVGVSPEFEVALYTLCFFLGGEDNHVELGPYAV 420

Query: 421 NIKCYRLGNKIGSVFPVAE 440
           NIKCYR GNK+GSVFP+AE
Sbjct: 421 NIKCYRFGNKMGSVFPIAE 428

BLAST of CmaCh06G008300 vs. ExPASy TrEMBL
Match: A0A1S3BLB7 (poly(U)-specific endoribonuclease-B OS=Cucumis melo OX=3656 GN=LOC103490825 PE=3 SV=1)

HSP 1 Score: 734.9 bits (1896), Expect = 1.9e-208
Identity = 372/439 (84.74%), Postives = 395/439 (89.98%), Query Frame = 0

Query: 1   MEGLIKGLIDVAIGHDDDNNNSNEQSAPQSRDERSRSTWAQVVTGEEDADQDGGDRGHDR 60
           MEGLIKGLIDVAIG+D DNN  ++QSA QSR+ERSRSTWAQVVTGEED        G DR
Sbjct: 1   MEGLIKGLIDVAIGNDGDNN--DQQSASQSREERSRSTWAQVVTGEED--------GGDR 60

Query: 61  RHGNDWNRRDNVKQGRSRREEEWEVEGSRISGQRQQGEYGSEHGYNRDQWAKKESSEENN 120
           R G D NR +N ++GRSRR EEWEVE SRISGQR+Q EYGSEHGYNR+QWAK+E SEE N
Sbjct: 61  RDGCDRNRWNNEEEGRSRR-EEWEVEDSRISGQRKQSEYGSEHGYNRNQWAKEEGSEEKN 120

Query: 121 DGWETVHKKAPRKHHKVQVENWEGYKRPASEQNYSDEVEVGAELEPSEDELADFARACNK 180
           DGWETVHKK  R+ HK+Q +NW GYKRPASEQNYS+EVEVGAELEPSEDELAD + AC K
Sbjct: 121 DGWETVHKKPTRRQHKIQTDNWGGYKRPASEQNYSNEVEVGAELEPSEDELADLSYACKK 180

Query: 181 LWELDLNRLVPGQDYEIDCGEGKRVHGREDMAQASLFSWVNEDVFRKPTFSRFCSLLDNY 240
           LW+LDLNRLVPG+DYEIDCGEGKRV GREDMAQ SLFSWV+EDVF KPTFSRFCSLLDNY
Sbjct: 181 LWQLDLNRLVPGKDYEIDCGEGKRVSGREDMAQGSLFSWVSEDVFGKPTFSRFCSLLDNY 240

Query: 241 NPDQGSKEVVTPEERQEQAAFIEEISRTAPIKYLHKYLLYKGVASNDYQDFKRMLTSLWF 300
           NP+QG KEVVTPEERQEQAAFIEEISRTAPIKYLH+YL  KGVASNDYQDFKRMLTSLWF
Sbjct: 241 NPNQGCKEVVTPEERQEQAAFIEEISRTAPIKYLHRYLSSKGVASNDYQDFKRMLTSLWF 300

Query: 301 DLYGRGGTSHSSSAFEHVFVGEIKQQGGQEVSGFHNWLQFYLEEAKGSVDYQGYIFPRRR 360
           DL GR GTS SSSAFEHVFVGEIKQQG QEVSGFHNWLQFYLEEAKGSVDYQGYIFPRRR
Sbjct: 301 DLCGRCGTSGSSSAFEHVFVGEIKQQGEQEVSGFHNWLQFYLEEAKGSVDYQGYIFPRRR 360

Query: 361 GQFPDSETQLLTIQFEWNGVLKSVSSTLVGVSPEFELALYTLCFFLGGEDNHVELGPYPV 420
           GQ PDSETQLLTIQFEWNG+LKSVSSTLVGVSPEFE+ALYTLCFFLGGEDNHVELGPY V
Sbjct: 361 GQCPDSETQLLTIQFEWNGILKSVSSTLVGVSPEFEVALYTLCFFLGGEDNHVELGPYAV 420

Query: 421 NIKCYRLGNKIGSVFPVAE 440
           NIKCYRLGN IGSVFP+AE
Sbjct: 421 NIKCYRLGNNIGSVFPIAE 428

BLAST of CmaCh06G008300 vs. NCBI nr
Match: XP_023005408.1 (poly(U)-specific endoribonuclease-B-like [Cucurbita maxima])

HSP 1 Score: 899.0 bits (2322), Expect = 1.6e-257
Identity = 440/440 (100.00%), Postives = 440/440 (100.00%), Query Frame = 0

Query: 1   MEGLIKGLIDVAIGHDDDNNNSNEQSAPQSRDERSRSTWAQVVTGEEDADQDGGDRGHDR 60
           MEGLIKGLIDVAIGHDDDNNNSNEQSAPQSRDERSRSTWAQVVTGEEDADQDGGDRGHDR
Sbjct: 1   MEGLIKGLIDVAIGHDDDNNNSNEQSAPQSRDERSRSTWAQVVTGEEDADQDGGDRGHDR 60

Query: 61  RHGNDWNRRDNVKQGRSRREEEWEVEGSRISGQRQQGEYGSEHGYNRDQWAKKESSEENN 120
           RHGNDWNRRDNVKQGRSRREEEWEVEGSRISGQRQQGEYGSEHGYNRDQWAKKESSEENN
Sbjct: 61  RHGNDWNRRDNVKQGRSRREEEWEVEGSRISGQRQQGEYGSEHGYNRDQWAKKESSEENN 120

Query: 121 DGWETVHKKAPRKHHKVQVENWEGYKRPASEQNYSDEVEVGAELEPSEDELADFARACNK 180
           DGWETVHKKAPRKHHKVQVENWEGYKRPASEQNYSDEVEVGAELEPSEDELADFARACNK
Sbjct: 121 DGWETVHKKAPRKHHKVQVENWEGYKRPASEQNYSDEVEVGAELEPSEDELADFARACNK 180

Query: 181 LWELDLNRLVPGQDYEIDCGEGKRVHGREDMAQASLFSWVNEDVFRKPTFSRFCSLLDNY 240
           LWELDLNRLVPGQDYEIDCGEGKRVHGREDMAQASLFSWVNEDVFRKPTFSRFCSLLDNY
Sbjct: 181 LWELDLNRLVPGQDYEIDCGEGKRVHGREDMAQASLFSWVNEDVFRKPTFSRFCSLLDNY 240

Query: 241 NPDQGSKEVVTPEERQEQAAFIEEISRTAPIKYLHKYLLYKGVASNDYQDFKRMLTSLWF 300
           NPDQGSKEVVTPEERQEQAAFIEEISRTAPIKYLHKYLLYKGVASNDYQDFKRMLTSLWF
Sbjct: 241 NPDQGSKEVVTPEERQEQAAFIEEISRTAPIKYLHKYLLYKGVASNDYQDFKRMLTSLWF 300

Query: 301 DLYGRGGTSHSSSAFEHVFVGEIKQQGGQEVSGFHNWLQFYLEEAKGSVDYQGYIFPRRR 360
           DLYGRGGTSHSSSAFEHVFVGEIKQQGGQEVSGFHNWLQFYLEEAKGSVDYQGYIFPRRR
Sbjct: 301 DLYGRGGTSHSSSAFEHVFVGEIKQQGGQEVSGFHNWLQFYLEEAKGSVDYQGYIFPRRR 360

Query: 361 GQFPDSETQLLTIQFEWNGVLKSVSSTLVGVSPEFELALYTLCFFLGGEDNHVELGPYPV 420
           GQFPDSETQLLTIQFEWNGVLKSVSSTLVGVSPEFELALYTLCFFLGGEDNHVELGPYPV
Sbjct: 361 GQFPDSETQLLTIQFEWNGVLKSVSSTLVGVSPEFELALYTLCFFLGGEDNHVELGPYPV 420

Query: 421 NIKCYRLGNKIGSVFPVAES 441
           NIKCYRLGNKIGSVFPVAES
Sbjct: 421 NIKCYRLGNKIGSVFPVAES 440

BLAST of CmaCh06G008300 vs. NCBI nr
Match: XP_023539783.1 (poly(U)-specific endoribonuclease-B [Cucurbita pepo subsp. pepo])

HSP 1 Score: 882.1 bits (2278), Expect = 2.0e-252
Identity = 432/440 (98.18%), Postives = 435/440 (98.86%), Query Frame = 0

Query: 1   MEGLIKGLIDVAIGHDDDNNNSNEQSAPQSRDERSRSTWAQVVTGEEDADQDGGDRGHDR 60
           MEGLIKGLIDVAIGHDDDNNN+NEQSAPQSRDERSRSTWAQVVTGEEDAD+DGGDRGHDR
Sbjct: 1   MEGLIKGLIDVAIGHDDDNNNNNEQSAPQSRDERSRSTWAQVVTGEEDADRDGGDRGHDR 60

Query: 61  RHGNDWNRRDNVKQGRSRREEEWEVEGSRISGQRQQGEYGSEHGYNRDQWAKKESSEENN 120
           RHGND NRRDN K+GRSRREEEWEVEGSRISGQRQQGEYGSEHGYNRDQWAKKE SEENN
Sbjct: 61  RHGNDSNRRDNEKEGRSRREEEWEVEGSRISGQRQQGEYGSEHGYNRDQWAKKEGSEENN 120

Query: 121 DGWETVHKKAPRKHHKVQVENWEGYKRPASEQNYSDEVEVGAELEPSEDELADFARACNK 180
           DGWETVHKKAPRKHHKVQVENWEGYKRPASEQNYSDEVEVGAELEPSEDELADFARACNK
Sbjct: 121 DGWETVHKKAPRKHHKVQVENWEGYKRPASEQNYSDEVEVGAELEPSEDELADFARACNK 180

Query: 181 LWELDLNRLVPGQDYEIDCGEGKRVHGREDMAQASLFSWVNEDVFRKPTFSRFCSLLDNY 240
           LWELDLNRLVPGQDYEIDCGEGKRVHGREDMAQASLFSWVNEDVFRKPTFSRFCSLLDNY
Sbjct: 181 LWELDLNRLVPGQDYEIDCGEGKRVHGREDMAQASLFSWVNEDVFRKPTFSRFCSLLDNY 240

Query: 241 NPDQGSKEVVTPEERQEQAAFIEEISRTAPIKYLHKYLLYKGVASNDYQDFKRMLTSLWF 300
           NPDQGSKEVVTPEERQEQAAFIEEISRTAPIKYLHKYL YKGV SNDYQDFKRMLTSLWF
Sbjct: 241 NPDQGSKEVVTPEERQEQAAFIEEISRTAPIKYLHKYLSYKGVTSNDYQDFKRMLTSLWF 300

Query: 301 DLYGRGGTSHSSSAFEHVFVGEIKQQGGQEVSGFHNWLQFYLEEAKGSVDYQGYIFPRRR 360
           DLYGRGGTSHSSSAFEHVFVGEIKQQGGQEVSGFHNWLQFYLEEAKGSVDYQGYIFPRRR
Sbjct: 301 DLYGRGGTSHSSSAFEHVFVGEIKQQGGQEVSGFHNWLQFYLEEAKGSVDYQGYIFPRRR 360

Query: 361 GQFPDSETQLLTIQFEWNGVLKSVSSTLVGVSPEFELALYTLCFFLGGEDNHVELGPYPV 420
           GQFPDSETQLLTIQFEWNGVLKSVSSTLVGVSPEFELALYTLCFFLGGEDNHVELGPYPV
Sbjct: 361 GQFPDSETQLLTIQFEWNGVLKSVSSTLVGVSPEFELALYTLCFFLGGEDNHVELGPYPV 420

Query: 421 NIKCYRLGNKIGSVFPVAES 441
           NIKCYRLGNKIGSVFPVAES
Sbjct: 421 NIKCYRLGNKIGSVFPVAES 440

BLAST of CmaCh06G008300 vs. NCBI nr
Match: KAG7030180.1 (Poly(U)-specific endoribonuclease-B [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 867.1 bits (2239), Expect = 6.6e-248
Identity = 427/440 (97.05%), Postives = 433/440 (98.41%), Query Frame = 0

Query: 1   MEGLIKGLIDVAIGHDDDNNNSNEQSAPQSRDERSRSTWAQVVTGEEDADQDGGDRGHDR 60
           MEGLIKGLIDVAIGHDDDNN  NEQSA QSRDERSRSTWAQVVTGEEDAD+DGGDRGHDR
Sbjct: 1   MEGLIKGLIDVAIGHDDDNN--NEQSASQSRDERSRSTWAQVVTGEEDADRDGGDRGHDR 60

Query: 61  RHGNDWNRRDNVKQGRSRREEEWEVEGSRISGQRQQGEYGSEHGYNRDQWAKKESSEENN 120
           +HGNDWNRRDN K+GRSRR EEWEV+GSRISGQRQQGEYGSEHGYNRDQWAKKESSEENN
Sbjct: 61  KHGNDWNRRDNEKEGRSRR-EEWEVQGSRISGQRQQGEYGSEHGYNRDQWAKKESSEENN 120

Query: 121 DGWETVHKKAPRKHHKVQVENWEGYKRPASEQNYSDEVEVGAELEPSEDELADFARACNK 180
           DGWETVHKKAPRKHHKVQVENWEGYKRPASEQNYSDEVEVGAELEPSEDELADFARACNK
Sbjct: 121 DGWETVHKKAPRKHHKVQVENWEGYKRPASEQNYSDEVEVGAELEPSEDELADFARACNK 180

Query: 181 LWELDLNRLVPGQDYEIDCGEGKRVHGREDMAQASLFSWVNEDVFRKPTFSRFCSLLDNY 240
           LWELDLNRLVPGQDYEIDCGEGKRVHGREDMAQASLFSWV+EDVFRKPTFSRFCSLLDNY
Sbjct: 181 LWELDLNRLVPGQDYEIDCGEGKRVHGREDMAQASLFSWVSEDVFRKPTFSRFCSLLDNY 240

Query: 241 NPDQGSKEVVTPEERQEQAAFIEEISRTAPIKYLHKYLLYKGVASNDYQDFKRMLTSLWF 300
           NPDQGSKEVVTPEE+QEQAAFIEEISRTAPIKYLHKYL YKGV SNDYQDFKRMLTSLWF
Sbjct: 241 NPDQGSKEVVTPEEKQEQAAFIEEISRTAPIKYLHKYLSYKGVTSNDYQDFKRMLTSLWF 300

Query: 301 DLYGRGGTSHSSSAFEHVFVGEIKQQGGQEVSGFHNWLQFYLEEAKGSVDYQGYIFPRRR 360
           DLYGRGGTSHSSSAFEHVFVGEIKQQGGQEVSGFHNWLQFYLEEAKGSVDYQGYIFPRRR
Sbjct: 301 DLYGRGGTSHSSSAFEHVFVGEIKQQGGQEVSGFHNWLQFYLEEAKGSVDYQGYIFPRRR 360

Query: 361 GQFPDSETQLLTIQFEWNGVLKSVSSTLVGVSPEFELALYTLCFFLGGEDNHVELGPYPV 420
           GQFPDSETQLLTIQFEWNGVLKSVSSTLVGVSPEFELALYTLCFFLGGEDNHVELGPYPV
Sbjct: 361 GQFPDSETQLLTIQFEWNGVLKSVSSTLVGVSPEFELALYTLCFFLGGEDNHVELGPYPV 420

Query: 421 NIKCYRLGNKIGSVFPVAES 441
           NIKCYRLGNKIGSVFPVAES
Sbjct: 421 NIKCYRLGNKIGSVFPVAES 437

BLAST of CmaCh06G008300 vs. NCBI nr
Match: XP_022936491.1 (poly(U)-specific endoribonuclease-B-like [Cucurbita moschata])

HSP 1 Score: 864.4 bits (2232), Expect = 4.3e-247
Identity = 426/440 (96.82%), Postives = 432/440 (98.18%), Query Frame = 0

Query: 1   MEGLIKGLIDVAIGHDDDNNNSNEQSAPQSRDERSRSTWAQVVTGEEDADQDGGDRGHDR 60
           MEGLIKGLIDVAIGHDDD N  NEQSA QSRDERSRSTWAQVVTGEEDAD+DGGDRGHDR
Sbjct: 1   MEGLIKGLIDVAIGHDDDKN--NEQSASQSRDERSRSTWAQVVTGEEDADRDGGDRGHDR 60

Query: 61  RHGNDWNRRDNVKQGRSRREEEWEVEGSRISGQRQQGEYGSEHGYNRDQWAKKESSEENN 120
           RHGNDWNRRDN K+GRSRR EEWEV+GSRISGQ+QQGEYGSEHGYNRDQWAKKESSEENN
Sbjct: 61  RHGNDWNRRDNEKEGRSRR-EEWEVQGSRISGQQQQGEYGSEHGYNRDQWAKKESSEENN 120

Query: 121 DGWETVHKKAPRKHHKVQVENWEGYKRPASEQNYSDEVEVGAELEPSEDELADFARACNK 180
           DGWETVHKKAPRKHHKVQVENWEGYKRPASEQNYSDEVEVGAELEPSEDELADFARACNK
Sbjct: 121 DGWETVHKKAPRKHHKVQVENWEGYKRPASEQNYSDEVEVGAELEPSEDELADFARACNK 180

Query: 181 LWELDLNRLVPGQDYEIDCGEGKRVHGREDMAQASLFSWVNEDVFRKPTFSRFCSLLDNY 240
           LWELDLNRLVPGQDYEIDCGEGKRVHGREDMAQASLFSWV+EDVFRKPTFSRFCSLLDNY
Sbjct: 181 LWELDLNRLVPGQDYEIDCGEGKRVHGREDMAQASLFSWVSEDVFRKPTFSRFCSLLDNY 240

Query: 241 NPDQGSKEVVTPEERQEQAAFIEEISRTAPIKYLHKYLLYKGVASNDYQDFKRMLTSLWF 300
           NPDQGSKEVVTPEE+QEQAAFIEEISRTAPIKYLHKYL YKGV SNDYQDFKRMLTSLWF
Sbjct: 241 NPDQGSKEVVTPEEKQEQAAFIEEISRTAPIKYLHKYLSYKGVTSNDYQDFKRMLTSLWF 300

Query: 301 DLYGRGGTSHSSSAFEHVFVGEIKQQGGQEVSGFHNWLQFYLEEAKGSVDYQGYIFPRRR 360
           DLYGRGGTSHSSSAFEHVFVGEIKQQGGQEVSGFHNWLQFYLEEAKGSVDYQGYIFPRRR
Sbjct: 301 DLYGRGGTSHSSSAFEHVFVGEIKQQGGQEVSGFHNWLQFYLEEAKGSVDYQGYIFPRRR 360

Query: 361 GQFPDSETQLLTIQFEWNGVLKSVSSTLVGVSPEFELALYTLCFFLGGEDNHVELGPYPV 420
           GQFPDSETQLLTIQFEWNGVLKSVSSTLVGVSPEFELALYTLCFFLGGEDNHVELGPYPV
Sbjct: 361 GQFPDSETQLLTIQFEWNGVLKSVSSTLVGVSPEFELALYTLCFFLGGEDNHVELGPYPV 420

Query: 421 NIKCYRLGNKIGSVFPVAES 441
           NIKCYRLGNKIGSVFPVAES
Sbjct: 421 NIKCYRLGNKIGSVFPVAES 437

BLAST of CmaCh06G008300 vs. NCBI nr
Match: KAG6596904.1 (Poly(U)-specific endoribonuclease-B, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 863.6 bits (2230), Expect = 7.3e-247
Identity = 425/440 (96.59%), Postives = 432/440 (98.18%), Query Frame = 0

Query: 1   MEGLIKGLIDVAIGHDDDNNNSNEQSAPQSRDERSRSTWAQVVTGEEDADQDGGDRGHDR 60
           MEGLIKGLIDVAIGHDDDNN  NEQSA QSRDERSRSTWAQVVTGEEDAD+DGGDRGHDR
Sbjct: 1   MEGLIKGLIDVAIGHDDDNN--NEQSASQSRDERSRSTWAQVVTGEEDADRDGGDRGHDR 60

Query: 61  RHGNDWNRRDNVKQGRSRREEEWEVEGSRISGQRQQGEYGSEHGYNRDQWAKKESSEENN 120
           +HGNDWNRRDN K+GRSRR EEWEV+GSRISGQRQQGEYGSEHGYNRDQWAKKESSEENN
Sbjct: 61  KHGNDWNRRDNEKEGRSRR-EEWEVQGSRISGQRQQGEYGSEHGYNRDQWAKKESSEENN 120

Query: 121 DGWETVHKKAPRKHHKVQVENWEGYKRPASEQNYSDEVEVGAELEPSEDELADFARACNK 180
           DGWETVHKKAPRKHHKVQVENWEGYKRPASEQNYSDEVEVGAELEPSEDELADFARACNK
Sbjct: 121 DGWETVHKKAPRKHHKVQVENWEGYKRPASEQNYSDEVEVGAELEPSEDELADFARACNK 180

Query: 181 LWELDLNRLVPGQDYEIDCGEGKRVHGREDMAQASLFSWVNEDVFRKPTFSRFCSLLDNY 240
           LWELDLNRLVPGQDYEIDCGEGKRVHGREDMAQASLFSWV+EDVFRKPTFSRFCSLLDNY
Sbjct: 181 LWELDLNRLVPGQDYEIDCGEGKRVHGREDMAQASLFSWVSEDVFRKPTFSRFCSLLDNY 240

Query: 241 NPDQGSKEVVTPEERQEQAAFIEEISRTAPIKYLHKYLLYKGVASNDYQDFKRMLTSLWF 300
           NPDQGSKEVVTPEE+QEQAAFIEEISRTAPIKYLHKYL YKGV SNDYQDFKRMLTSLWF
Sbjct: 241 NPDQGSKEVVTPEEKQEQAAFIEEISRTAPIKYLHKYLSYKGVTSNDYQDFKRMLTSLWF 300

Query: 301 DLYGRGGTSHSSSAFEHVFVGEIKQQGGQEVSGFHNWLQFYLEEAKGSVDYQGYIFPRRR 360
           DLYGRGGT HSSSAFEHVFVGEIKQQGGQEVSGFHNWLQFYLEEAKGSVDYQGYIFPRRR
Sbjct: 301 DLYGRGGTCHSSSAFEHVFVGEIKQQGGQEVSGFHNWLQFYLEEAKGSVDYQGYIFPRRR 360

Query: 361 GQFPDSETQLLTIQFEWNGVLKSVSSTLVGVSPEFELALYTLCFFLGGEDNHVELGPYPV 420
           GQFPDSETQLLTIQFEWNGVLKSVSSTLVGVSPEFELALYTLCFFLGGEDNHVELGPYPV
Sbjct: 361 GQFPDSETQLLTIQFEWNGVLKSVSSTLVGVSPEFELALYTLCFFLGGEDNHVELGPYPV 420

Query: 421 NIKCYRLGNKIGSVFPVAES 441
           NIKCY+LGNKIGSVFPVAES
Sbjct: 421 NIKCYQLGNKIGSVFPVAES 437

BLAST of CmaCh06G008300 vs. TAIR 10
Match: AT4G17100.1 (CONTAINS InterPro DOMAIN/s: Endoribonuclease XendoU (InterPro:IPR018998); Has 943 Blast hits to 770 proteins in 162 species: Archae - 0; Bacteria - 61; Metazoa - 472; Fungi - 40; Plants - 78; Viruses - 35; Other Eukaryotes - 257 (source: NCBI BLink). )

HSP 1 Score: 580.9 bits (1496), Expect = 8.8e-166
Identity = 303/451 (67.18%), Postives = 351/451 (77.83%), Query Frame = 0

Query: 1   MEGLIKGLIDVAIGHDD----DNNNSNEQSAPQSRDERSRSTWAQVVTGEEDADQDGGDR 60
           M+GLI+GL+DVAIG +     D+   +E +   SRDERSRSTWA VV+GEE+     G  
Sbjct: 1   MDGLIRGLVDVAIGGNQRRGGDDGGDDEGN---SRDERSRSTWANVVSGEEEDQNRAGGS 60

Query: 61  GHDRRHGNDWNRRDNVKQGRSRREEEWEVEGSRISGQRQQG--EYGSEHGYNRDQWAKK- 120
            H RR  ++              E +WE +G R+  +  Q   E   E+ Y      K+ 
Sbjct: 61  SHGRRQNSE--------------ENQWEKKGERLPTRHSQSHEEDQEENTYAAKVSEKQD 120

Query: 121 --ESSEENNDGWETVHKKAP-RKHHKVQVENWEGYKRPASEQNYSDEVEVGAELEPSEDE 180
             ES +E NDGWETV KK P R+ HKVQ E W+ YKRPASEQ+YSDEVE    LEPS+ E
Sbjct: 121 FSESQQEENDGWETVGKKKPARQSHKVQKEQWQDYKRPASEQHYSDEVETYGNLEPSQLE 180

Query: 181 LADFARACNKLWELDLNRLVPGQDYEIDCGEGKRVHGREDMAQASLFSWVNEDVFRKPTF 240
           L+  + ACNKLWELDLNRLVPG+DY+IDCG+GKRVH R DMA+  LFSWV+E+V RKPTF
Sbjct: 181 LSGLSEACNKLWELDLNRLVPGRDYQIDCGDGKRVHERADMAEGLLFSWVSEEVSRKPTF 240

Query: 241 SRFCSLLDNYNPDQGSKEVVTPEERQEQAAFIEEISRTAPIKYLHKYLLYKGVASNDYQD 300
           +RFCSLLDNYNP++G KEVVT EERQEQAAFIEEISRT+ IKYLH+YL+ K VA   Y +
Sbjct: 241 ARFCSLLDNYNPNEGYKEVVTEEERQEQAAFIEEISRTSVIKYLHRYLVLKDVAPGSYLE 300

Query: 301 FKRMLTSLWFDLYGRGGTSHSSSAFEHVFVGEIKQQGGQEVSGFHNWLQFYLEEAKGSVD 360
           FKRMLTSLWFDLYGRGGTS SSSAFEHVFVGEIKQ GG++VSGFHNWLQFYLEEAKG+VD
Sbjct: 301 FKRMLTSLWFDLYGRGGTSGSSSAFEHVFVGEIKQSGGEQVSGFHNWLQFYLEEAKGTVD 360

Query: 361 YQGYIFPRRRGQFPDSETQLLTIQFEWNGVLKSVSSTLVGVSPEFELALYTLCFFLGGED 420
           YQGYIFPRRRG+ PDSETQLLTIQFEWNGVLKSVSSTLVGVSPEFELALYT+CFF+G ED
Sbjct: 361 YQGYIFPRRRGEIPDSETQLLTIQFEWNGVLKSVSSTLVGVSPEFELALYTMCFFMGTED 420

Query: 421 NHVELGPYPVNIKCYRLG-NKIGSVFPVAES 441
           NH++LGPY VN+KCYRLG N+IGS FP+AES
Sbjct: 421 NHIQLGPYNVNVKCYRLGNNRIGSAFPIAES 434

BLAST of CmaCh06G008300 vs. TAIR 10
Match: AT4G17100.2 (EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Endoribonuclease XendoU (InterPro:IPR018998). )

HSP 1 Score: 547.0 bits (1408), Expect = 1.4e-155
Identity = 288/451 (63.86%), Postives = 336/451 (74.50%), Query Frame = 0

Query: 1   MEGLIKGLIDVAIGHDDDNNNSNEQSAPQSRDERSRST----WAQVVTGEEDADQDGGDR 60
           M+GLI+GL+D     ++ N  +            +R         VV+GEE+     G  
Sbjct: 1   MDGLIRGLVDSTWLSEETNAAAETMVVMMKATRGTRDPDLLGLMFVVSGEEEDQNRAGGS 60

Query: 61  GHDRRHGNDWNRRDNVKQGRSRREEEWEVEGSRISGQRQQG--EYGSEHGYNRDQWAKK- 120
            H RR  ++              E +WE +G R+  +  Q   E   E+ Y      K+ 
Sbjct: 61  SHGRRQNSE--------------ENQWEKKGERLPTRHSQSHEEDQEENTYAAKVSEKQD 120

Query: 121 --ESSEENNDGWETVHKKAP-RKHHKVQVENWEGYKRPASEQNYSDEVEVGAELEPSEDE 180
             ES +E NDGWETV KK P R+ HKVQ E W+ YKRPASEQ+YSDEVE    LEPS+ E
Sbjct: 121 FSESQQEENDGWETVGKKKPARQSHKVQKEQWQDYKRPASEQHYSDEVETYGNLEPSQLE 180

Query: 181 LADFARACNKLWELDLNRLVPGQDYEIDCGEGKRVHGREDMAQASLFSWVNEDVFRKPTF 240
           L+  + ACNKLWELDLNRLVPG+DY+IDCG+GKRVH R DMA+  LFSWV+E+V RKPTF
Sbjct: 181 LSGLSEACNKLWELDLNRLVPGRDYQIDCGDGKRVHERADMAEGLLFSWVSEEVSRKPTF 240

Query: 241 SRFCSLLDNYNPDQGSKEVVTPEERQEQAAFIEEISRTAPIKYLHKYLLYKGVASNDYQD 300
           +RFCSLLDNYNP++G KEVVT EERQEQAAFIEEISRT+ IKYLH+YL+ K VA   Y +
Sbjct: 241 ARFCSLLDNYNPNEGYKEVVTEEERQEQAAFIEEISRTSVIKYLHRYLVLKDVAPGSYLE 300

Query: 301 FKRMLTSLWFDLYGRGGTSHSSSAFEHVFVGEIKQQGGQEVSGFHNWLQFYLEEAKGSVD 360
           FKRMLTSLWFDLYGRGGTS SSSAFEHVFVGEIKQ GG++VSGFHNWLQFYLEEAKG+VD
Sbjct: 301 FKRMLTSLWFDLYGRGGTSGSSSAFEHVFVGEIKQSGGEQVSGFHNWLQFYLEEAKGTVD 360

Query: 361 YQGYIFPRRRGQFPDSETQLLTIQFEWNGVLKSVSSTLVGVSPEFELALYTLCFFLGGED 420
           YQGYIFPRRRG+ PDSETQLLTIQFEWNGVLKSVSSTLVGVSPEFELALYT+CFF+G ED
Sbjct: 361 YQGYIFPRRRGEIPDSETQLLTIQFEWNGVLKSVSSTLVGVSPEFELALYTMCFFMGTED 420

Query: 421 NHVELGPYPVNIKCYRLG-NKIGSVFPVAES 441
           NH++LGPY VN+KCYRLG N+IGS FP+AES
Sbjct: 421 NHIQLGPYNVNVKCYRLGNNRIGSAFPIAES 437

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q503V92.3e-5744.91Poly(U)-specific endoribonuclease-B OS=Danio rerio OX=7955 GN=endoub PE=2 SV=1[more]
B1WBB44.4e-5341.82Poly(U)-specific endoribonuclease-B OS=Xenopus laevis OX=8355 GN=endou-b PE=2 SV... [more]
Q8JFY99.8e-5342.03Poly(U)-specific endoribonuclease-A OS=Xenopus laevis OX=8355 GN=endou-a PE=1 SV... [more]
B1H3D52.2e-5241.30Poly(U)-specific endoribonuclease OS=Xenopus tropicalis OX=8364 GN=endou PE=2 SV... [more]
Q1LUM31.6e-4740.96Poly(U)-specific endoribonuclease-C OS=Danio rerio OX=7955 GN=endouc PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1L2407.6e-258100.00poly(U)-specific endoribonuclease-B-like OS=Cucurbita maxima OX=3661 GN=LOC11149... [more]
A0A6J1F7L92.1e-24796.82poly(U)-specific endoribonuclease-B-like OS=Cucurbita moschata OX=3662 GN=LOC111... [more]
A0A6J1DMN31.3e-21283.86poly(U)-specific endoribonuclease-B-like OS=Momordica charantia OX=3673 GN=LOC11... [more]
A0A0A0L6P15.3e-21185.42XendoU domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G017000 PE=3... [more]
A0A1S3BLB71.9e-20884.74poly(U)-specific endoribonuclease-B OS=Cucumis melo OX=3656 GN=LOC103490825 PE=3... [more]
Match NameE-valueIdentityDescription
XP_023005408.11.6e-257100.00poly(U)-specific endoribonuclease-B-like [Cucurbita maxima][more]
XP_023539783.12.0e-25298.18poly(U)-specific endoribonuclease-B [Cucurbita pepo subsp. pepo][more]
KAG7030180.16.6e-24897.05Poly(U)-specific endoribonuclease-B [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_022936491.14.3e-24796.82poly(U)-specific endoribonuclease-B-like [Cucurbita moschata][more]
KAG6596904.17.3e-24796.59Poly(U)-specific endoribonuclease-B, partial [Cucurbita argyrosperma subsp. soro... [more]
Match NameE-valueIdentityDescription
AT4G17100.18.8e-16667.18CONTAINS InterPro DOMAIN/s: Endoribonuclease XendoU (InterPro:IPR018998); Has 94... [more]
AT4G17100.21.4e-15563.86EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS ... [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR018998EndoU ribonuclease, C-terminalPFAMPF09412XendoUcoord: 173..436
e-value: 2.3E-99
score: 332.0
IPR018998EndoU ribonuclease, C-terminalCDDcd21159XendoUcoord: 170..436
e-value: 1.94318E-127
score: 367.641
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..136
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 16..44
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 105..128
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 49..88
NoneNo IPR availablePANTHERPTHR12439:SF34SUBFAMILY NOT NAMEDcoord: 88..439
IPR039787Poly(U)-specific endoribonucleasePANTHERPTHR12439PLACENTAL PROTEIN 11-RELATEDcoord: 88..439
IPR037227Endoribonuclease EndoU-likeSUPERFAMILY142877EndoU-likecoord: 173..438

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh06G008300.1CmaCh06G008300.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0032259 methylation
biological_process GO:0090502 RNA phosphodiester bond hydrolysis, endonucleolytic
molecular_function GO:0004521 endoribonuclease activity
molecular_function GO:0008168 methyltransferase activity