CmaCh06G003750 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh06G003750
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
DescriptionPlasma membrane intrinsic protein
LocationCma_Chr06: 1736779 .. 1741766 (-)
RNA-Seq ExpressionCmaCh06G003750
SyntenyCmaCh06G003750
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATTAACAACTCTTTGCATGCCCCATAAAGTGTCGAACCAAATTCCAATTTCCAAATTCAAATCCATCTTTCTCAATCGGAGCTCACATTCACTCATCTAAGCTCACTTTCTCTCTGCTCCAATGGCGAATAACGACATCGAAGTCGGATCTCGGGGGTCCCTTCCGAGGAAGGACTATTTCGAGCCGCTTCCGGCGCCAATGATCGATATGGAGGAGTTGGCCAAATGGTCCTTTTACAGAGCCATAATCGCCGAGTTTGTCGCCACTCTTTTGTTTCTCTACGTCGTCGTTTTGACGGTCATCGGTAATAAAACCCAGACCGATCCAATCATCGGCGGTAACCTCTGCGGCGGAGTTGGCACCCTCGGAATCGCCTGGGTCTTCGGCGGTATGATCTTCGTTCTCGTTTACTGCACCGCCGGCATCTCTGGTAATTCCCTTCTCTTCATTTTCCCCCAATTTTCAAAACCCAGAAAATTAACCGGTTGGGTGTGCAGGAGGACACATAAACCCGGCGGTGACATTCGGAATGTTTCTGGGGAGAAAGATATCGTTGATTCGCGCCTTGCTCTACATTATAGCTCAGTGCTTGGGGGCGTTATGCGGCTGCGCCTTAGTCAAGTCACTACAGAGAGATCGCTACAACCACTATGGCGGTGCCGCCAACCAGCTCGTCGACGGCTACAGCAAGGGCACCGGTCTCGCCGTCGAGATCGTCGGCACCTTCGTCCTTCTCTACACCGTCTTCTCCGCCACCGATCCCAAACGCAACGCCCGAGATTCCTACGTTCCTGTGCTGGCTCCACTCCCCATTGGGTTCACTGTTTTCATGGTTCATCTCGCTACAATTCCGATCACTGGCACTGGCATCAATCCAGCTCGAAGCTTCGGCGCCGCCGTGATCATCAACGATAAAGAAATTTGGAAAGATCATGTATGTGTCCAGTTTTGAAGAAATCCCTTCTTGATTTCACGTTTTTTTTGCAACGAATTAGAGATTTGATTGTGATTGTAAATTGCAGTGGATCTTCTGGGTCGGGCCGCTGATCGGAGCAACCATTGCTACGATGTATTACCAGTATGTTCTTCGAGCCTCCGCCGTGAAAGCAATTGGATCCTTCAGAAGCACCCCACCAACTCGCCACTAAAGTTATTGACACAACACACCACAACCAATCTATTTTTGATAAGTAGGAGATAGGAATAAACCGAACCAAGAGGCGGTTTTTTTTTTTTAAACTTTTTTTTATTTTTTTTTAAAATAAGAAATAATTAGTTTTCAAATTTTTATAGGGAGAACACAATTTTATATAAGAGGATTCTTTGTATTTAATATATATATATATATTGTAATTATGGCTGTCTGATATTTATTTAATTATTAGTAAGTCTAATAGGCTCTAAAATTTTAATTTTGTGTATAATATACTTTTAATTTTTTTTAAAAAAATTGTATTTAAAATAAAATTTTAACTTTAACATAATATTTAAAAGATTTATTAAGTTTTAAAGAATTAAATTACTAGTTTAACCTTACAATTTTAAATTTGTATTAAATAAGTGTCTAAAGATTAAAAAAAAATTAATAGCTTGGAATCTATTAGATGAAAAAAATGGATTTATTTAAAAATAAATAAATTTTAGGATCTTATTAGATGGAAGTCTTAGTCAAGAGCATATTAAATAGATCTAAAAAATATATTTAATATTTTAAAAGATTATCGATCAATAACAAAATAAATAAATAAATAAATAACCGGCATTTAAAATATAAATATTTACTAAAAAAATAAAATCTTATAATTTCATAGGCAAATTGGTAATTTATCCCATAAATAGGAAATTCCCACTCCATCGAAGACGTTTTTCCTTCGACCCATCGGTTTAAAGGGCAGTCGCGATTCCGTCGGAATCTTCCAGTTTCTTCGCACCCGGCAATCTCTATCTACAGGTACAGATCACATTCGCTTCCTCTCTCTCTCTCTCTCTCTCTCTAACTACTATTCTTTTGACGATCCGCTATTCTGGAATTTCAATCTATGATTGCTTGATCTTGTTCAGTGATTTGTTATTCTGCTCTGTCCGATTGATTCTTCTACTTCATTTACCGGCAACTGCGCGCGCGCGTATTTTACTTTTTGTCCGCCGAATTTGGTGTGCGGCTGCTAGACAGTGGTTACTGACGAGATCTTTGTTGTCGAATCCTTTTGTCTTGTGTTTCCTGTTCGGTTTGGATAGATAGCAGAAATTTTCTAGATCTATTTGTTATCTTAGATTTGGACCGTGAATAATCAAGAAACATAGGCTTATACGCTTAATCGATCTGCGTCGACTCCCGCCGAGTTATGAATTCTGCTGCTTGCAAGTGTAATTAAGTGTTGGATTTCAGAATTTGAAACTTAAGATTCATTTGATCGAGGGAATTTATAGGTTTTCAGTTCTCGGTCTTACTTTTGACGCACACAATTGATTTACGATTTGCTATATAAGCAACTGAAACTCTTGTCTATGATTCGTAGTTGAAGAAACTTTTGGTGAACTAGGTTTTGGGTTTAGGTTATGAGAAGCATTCGAGACAAGGTTGTTTTATGTTCTGGTGCATGAGATCTCTAAGCTGACTAGGTTTGAAACTGAAGGTGAGGAAAATTCAATTATTTTCTTGTACTTTTCCTCCTAGGTAGTTAATGATAAAACTACGAAAGACTAGGAGTTTGGACTAAAATTAGAGTGTTAAGGAGAAAAAACATTTTTTCTATAGATTAGTTTTGAACTGCGTCATTATGATATAAAGACTATATTTGTTGAAATAACTCTAAAATAATGCACAAAATGAAACCAGCCGCAAATACTTATTTGCATCGGAAACCATGATGATTCGTTAAACGAGGGTTGGAATGACACTTTGAGGCAGCAACTTCTAAGCTTCAAGATTTATGGCTTCTTTGATTTCTAAGAGCAAGGCACAGGCCTCATGGCTTTTATGGAGGCTGTGCTGTATTCTTACAAGTATTAAATCATCTTATTTGTTGAATAACTCTATGAGCTGCTCTTGCGATACGATAGTTCATTACAACTTTAACTGCTAAGACTTCGGTTTAGAAATTGGTCCGAGTCAGCCTAAACATAGTTTATGGTCAACCAAAAAGTTAGACATTTGGATCCCCACCCCCACATATTGAACTAAAAAAAAAGGTTTGATTCAATTAATCATGATAATATCTGCAACGACATTTAGCAACGTAGTTTCTGCTGAGATTGTTTTTACCTGAGCAGGCCCTTCTTTTACTTGTATAAAAAAACATCTTCTTTTTGTAGGAGAATATGGCAACTAGGGGTCAAGGTGGTATCCAACAATTGCTTGCAGCAGAGCAAGAAGCTCAACACATTGTCAATGCTGCCAGAAATGGTAAACTGACTCTTTTATTTTGGTTTTGTTATATACTTTTTAACATTCATATGCATGTAAATTTGTAATTTGGTTCAACTAGTTCCTGGAAGCATGTAGGTTCAGTTCATAATTTGCTTGACACGAGTTTACAAGGACCATCATCTTCTCTTTTACTGCTATCGATTTACTTTTTTATTGTTGCTTTATGAAGTTGAACATAATCTATAACTCAATGACTTTGGTTTTCCTGTTTTTTTTAGCAAAATTGGCCAGACTGAAACAAGCCAAAGAAGAGGCTGAAAAGGAAATTGCTGCTTACCGTGCCCAAGTGGAGGCTGAGTTCCAAAGAAAACTCTCTGAGGTTGGTATTTAATTATATTATGCACTTAAAATTTTCTCTTGCTGTGAAAAAAGCCATCCTGAGATGTTAATATATCTGCTAACTTCTTTTCCTTTGGGGTCTTCCAGAATATATCTGCTATCCCATTTGGGACAGGGGTTGCCTTCTTTTGTTCATACTATCAGTGAAATGAAGGAATCATAGTTTCTCATAAAAAATATATATATATATAGTTGCTAACTCTGCCCTTCTTGCTTCCCCCATCTCACTATCATTTTTGGTCATATAGAGTAGCGGAGATTCAGGAGCTAATGTTAAGCGGCTTGAAGTCGAGACAGATGCAAAGATTGCGCACCTGAAAGAAGAGTGTGCTAGAATTTCATACGATGTCGTGGACATGCTCCTGAAGCATGTGACAACCGTGAAAAATTGAACGTCTTGATCATAAAGGTTGCAGTAAGAAACTCTAAATTATGTTATTCTTTTTGTGGTGCCTGGAAACGGCTCAAACTGCATGTGTATTATGGAGATGAATCCTTTCCTTTGTATGGATCAGTCAGTACTTTGCAGACTGCACTGTGTTGAATAATATATGATTTGATTCAATGTTTATTTCTATGTTTCATCACGTTGAATTGAATGTTTATGAAGTAACACATTCCTGTTGTCGTCTTGATTGATTTGCTTGATAGCTTCTTTATGCATCCAGGGTTCTTCCTTATCATCTTTGGATCAATATTTGAATGGAAGTTTGTGTTAGAAGTCCTTGGATGATTCGAACTTTGTAGACTAAGGTATACAAAATCCCTTTCGTGTACAGCATACCTTTAATGTGTACTACTATTATTTTACAGCATAAAATAAAGAAAAAGTTTGTGTTTGGGAATAAGGATTGTTATTCCTTTTCGGCATAATTTGTTTATTACTATTATTTATTATTCCTACAGGAAACTGTGGTCCTTCAATCAAATTCCAAGGTTCTTCCTTATCATCTTTATCGGTACACAACATGGCCAATATTCTGCTCTCCTTAATTTTCAAAATACGTTTTTATTTTTTATTTTTAATATAGGCTCAAAACTGATTGACGCATTAGATATAAATTGTTTGGATTGTATAGGTTATGAAATTCCATCTCTAGTTTGGGAGTTTTAGTTAGAAGGTTCTTCATTATGTGGTGCTCGGTTATCTCTCTCAAATATTTCAATTGAAGAGGACGAGTCATATCTAATTTGTCCCAAGGACAATGATGTCGTAAG

mRNA sequence

ATTAACAACTCTTTGCATGCCCCATAAAGTGTCGAACCAAATTCCAATTTCCAAATTCAAATCCATCTTTCTCAATCGGAGCTCACATTCACTCATCTAAGCTCACTTTCTCTCTGCTCCAATGGCGAATAACGACATCGAAGTCGGATCTCGGGGGTCCCTTCCGAGGAAGGACTATTTCGAGCCGCTTCCGGCGCCAATGATCGATATGGAGGAGTTGGCCAAATGGTCCTTTTACAGAGCCATAATCGCCGAGTTTGTCGCCACTCTTTTGTTTCTCTACGTCGTCGTTTTGACGGTCATCGGTAATAAAACCCAGACCGATCCAATCATCGGCGGTAACCTCTGCGGCGGAGTTGGCACCCTCGGAATCGCCTGGGTCTTCGGCGGTATGATCTTCGTTCTCGTTTACTGCACCGCCGGCATCTCTGGAGGACACATAAACCCGGCGGTGACATTCGGAATGTTTCTGGGGAGAAAGATATCGTTGATTCGCGCCTTGCTCTACATTATAGCTCAGTGCTTGGGGGCGTTATGCGGCTGCGCCTTAGTCAAGTCACTACAGAGAGATCGCTACAACCACTATGGCGGTGCCGCCAACCAGCTCGTCGACGGCTACAGCAAGGGCACCGGTCTCGCCGTCGAGATCGTCGGCACCTTCGTCCTTCTCTACACCGTCTTCTCCGCCACCGATCCCAAACGCAACGCCCGAGATTCCTACGTTCCTGTGCTGGCTCCACTCCCCATTGGGTTCACTGTTTTCATGGTTCATCTCGCTACAATTCCGATCACTGGCACTGGCATCAATCCAGCTCGAAGCTTCGGCGCCGCCGTGATCATCAACGATAAAGAAATTTGGAAAGATCATTGGATCTTCTGGGTCGGGCCGCTGATCGGAGCAACCATTGCTACGATGTATTACCAGGCAGTCGCGATTCCGTCGGAATCTTCCAGTTTCTTCGCACCCGGCAATCTCTATCTACAGGAGAATATGGCAACTAGGGGTCAAGGTGGTATCCAACAATTGCTTGCAGCAGAGCAAGAAGCTCAACACATTGTCAATGCTGCCAGAAATGCAAAATTGGCCAGACTGAAACAAGCCAAAGAAGAGGCTGAAAAGGAAATTGCTGCTTACCGTGCCCAAGTGGAGGCTGAGTTCCAAAGAAAACTCTCTGAGAGTAGCGGAGATTCAGGAGCTAATGTTAAGCGGCTTGAAGTCGAGACAGATGCAAAGATTGCGCACCTGAAAGAAGAGTGTGCTAGAATTTCATACGATGTCGTGGACATGCTCCTGAAGCATGTGACAACCGTGAAAAATTGAACGTCTTGATCATAAAGGGTTCTTCCTTATCATCTTTGGATCAATATTTGAATGGAAGTTTGTGTTAGAAGTCCTTGGATGATTCGAACTTTGTAGACTAAGGAAACTGTGGTCCTTCAATCAAATTCCAAGGTTCTTCCTTATCATCTTTATCGGTACACAACATGGCCAATATTCTGCTCTCCTTAATTTTCAAAATACGTTTTTATTTTTTATTTTTAATATAGGCTCAAAACTGATTGACGCATTAGATATAAATTGTTTGGATTGTATAGGTTATGAAATTCCATCTCTAGTTTGGGAGTTTTAGTTAGAAGGTTCTTCATTATGTGGTGCTCGGTTATCTCTCTCAAATATTTCAATTGAAGAGGACGAGTCATATCTAATTTGTCCCAAGGACAATGATGTCGTAAG

Coding sequence (CDS)

ATGGCGAATAACGACATCGAAGTCGGATCTCGGGGGTCCCTTCCGAGGAAGGACTATTTCGAGCCGCTTCCGGCGCCAATGATCGATATGGAGGAGTTGGCCAAATGGTCCTTTTACAGAGCCATAATCGCCGAGTTTGTCGCCACTCTTTTGTTTCTCTACGTCGTCGTTTTGACGGTCATCGGTAATAAAACCCAGACCGATCCAATCATCGGCGGTAACCTCTGCGGCGGAGTTGGCACCCTCGGAATCGCCTGGGTCTTCGGCGGTATGATCTTCGTTCTCGTTTACTGCACCGCCGGCATCTCTGGAGGACACATAAACCCGGCGGTGACATTCGGAATGTTTCTGGGGAGAAAGATATCGTTGATTCGCGCCTTGCTCTACATTATAGCTCAGTGCTTGGGGGCGTTATGCGGCTGCGCCTTAGTCAAGTCACTACAGAGAGATCGCTACAACCACTATGGCGGTGCCGCCAACCAGCTCGTCGACGGCTACAGCAAGGGCACCGGTCTCGCCGTCGAGATCGTCGGCACCTTCGTCCTTCTCTACACCGTCTTCTCCGCCACCGATCCCAAACGCAACGCCCGAGATTCCTACGTTCCTGTGCTGGCTCCACTCCCCATTGGGTTCACTGTTTTCATGGTTCATCTCGCTACAATTCCGATCACTGGCACTGGCATCAATCCAGCTCGAAGCTTCGGCGCCGCCGTGATCATCAACGATAAAGAAATTTGGAAAGATCATTGGATCTTCTGGGTCGGGCCGCTGATCGGAGCAACCATTGCTACGATGTATTACCAGGCAGTCGCGATTCCGTCGGAATCTTCCAGTTTCTTCGCACCCGGCAATCTCTATCTACAGGAGAATATGGCAACTAGGGGTCAAGGTGGTATCCAACAATTGCTTGCAGCAGAGCAAGAAGCTCAACACATTGTCAATGCTGCCAGAAATGCAAAATTGGCCAGACTGAAACAAGCCAAAGAAGAGGCTGAAAAGGAAATTGCTGCTTACCGTGCCCAAGTGGAGGCTGAGTTCCAAAGAAAACTCTCTGAGAGTAGCGGAGATTCAGGAGCTAATGTTAAGCGGCTTGAAGTCGAGACAGATGCAAAGATTGCGCACCTGAAAGAAGAGTGTGCTAGAATTTCATACGATGTCGTGGACATGCTCCTGAAGCATGTGACAACCGTGAAAAATTGA

Protein sequence

MANNDIEVGSRGSLPRKDYFEPLPAPMIDMEELAKWSFYRAIIAEFVATLLFLYVVVLTVIGNKTQTDPIIGGNLCGGVGTLGIAWVFGGMIFVLVYCTAGISGGHINPAVTFGMFLGRKISLIRALLYIIAQCLGALCGCALVKSLQRDRYNHYGGAANQLVDGYSKGTGLAVEIVGTFVLLYTVFSATDPKRNARDSYVPVLAPLPIGFTVFMVHLATIPITGTGINPARSFGAAVIINDKEIWKDHWIFWVGPLIGATIATMYYQAVAIPSESSSFFAPGNLYLQENMATRGQGGIQQLLAAEQEAQHIVNAARNAKLARLKQAKEEAEKEIAAYRAQVEAEFQRKLSESSGDSGANVKRLEVETDAKIAHLKEECARISYDVVDMLLKHVTTVKN
Homology
BLAST of CmaCh06G003750 vs. ExPASy Swiss-Prot
Match: Q9FF53 (Probable aquaporin PIP2-4 OS=Arabidopsis thaliana OX=3702 GN=PIP2-4 PE=1 SV=1)

HSP 1 Score: 404.1 bits (1037), Expect = 1.9e-111
Identity = 193/266 (72.56%), Postives = 217/266 (81.58%), Query Frame = 0

Query: 5   DIEVGSRGSLPRKDYFEPLPAPMIDMEELAKWSFYRAIIAEFVATLLFLYVVVLTVIGNK 64
           D++V   G    +DY +P PAP  DMEEL KW  YRA+IAEFVATLLFLYV +LTVIG K
Sbjct: 4   DLDVNESGPPAARDYKDPPPAPFFDMEELRKWPLYRAVIAEFVATLLFLYVSILTVIGYK 63

Query: 65  TQTDPIIGGNLCGGVGTLGIAWVFGGMIFVLVYCTAGISGGHINPAVTFGMFLGRKISLI 124
            QTD   GG  CGGVG LGIAW FGGMIFVLVYCTAGISGGHINPAVT G+FL RK+SL+
Sbjct: 64  AQTDATAGGVDCGGVGILGIAWAFGGMIFVLVYCTAGISGGHINPAVTVGLFLARKVSLV 123

Query: 125 RALLYIIAQCLGALCGCALVKSLQRDRYNHYGGAANQLVDGYSKGTGLAVEIVGTFVLLY 184
           R +LYI+AQCLGA+CGC  VK+ Q   Y  YGG AN+L DGY+KGTGL  EI+GTFVL+Y
Sbjct: 124 RTVLYIVAQCLGAICGCGFVKAFQSSYYTRYGGGANELADGYNKGTGLGAEIIGTFVLVY 183

Query: 185 TVFSATDPKRNARDSYVPVLAPLPIGFTVFMVHLATIPITGTGINPARSFGAAVIINDKE 244
           TVFSATDPKRNARDS+VPVLAPLPIGF VFMVHLATIPITGTGINPARSFGAAVI N+++
Sbjct: 184 TVFSATDPKRNARDSHVPVLAPLPIGFAVFMVHLATIPITGTGINPARSFGAAVIYNNEK 243

Query: 245 IWKDHWIFWVGPLIGATIATMYYQAV 271
            W D WIFWVGP+IGA  A  Y+Q +
Sbjct: 244 AWDDQWIFWVGPMIGAAAAAFYHQFI 269

BLAST of CmaCh06G003750 vs. ExPASy Swiss-Prot
Match: P43286 (Aquaporin PIP2-1 OS=Arabidopsis thaliana OX=3702 GN=PIP2-1 PE=1 SV=1)

HSP 1 Score: 400.6 bits (1028), Expect = 2.1e-110
Identity = 200/274 (72.99%), Postives = 217/274 (79.20%), Query Frame = 0

Query: 5   DIEVGSRGSLPRKDYFEPLPAPMIDMEELAKWSFYRAIIAEFVATLLFLYVVVLTVIGNK 64
           D+E         +DY +P PAP ID  EL KWSFYRA+IAEFVATLLFLY+ VLTVIG K
Sbjct: 4   DVEAVPGEGFQTRDYQDPPPAPFIDGAELKKWSFYRAVIAEFVATLLFLYITVLTVIGYK 63

Query: 65  TQTDPIIGGNLCGGVGTLGIAWVFGGMIFVLVYCTAGISGGHINPAVTFGMFLGRKISLI 124
            Q+D   GG  CGGVG LGIAW FGGMIF+LVYCTAGISGGHINPAVTFG+FL RK+SL 
Sbjct: 64  IQSDTDAGGVDCGGVGILGIAWAFGGMIFILVYCTAGISGGHINPAVTFGLFLARKVSLP 123

Query: 125 RALLYIIAQCLGALCGCALVKSLQRDRYNHYGGAANQLVDGYSKGTGLAVEIVGTFVLLY 184
           RALLYIIAQCLGA+CG   VK+ Q   Y  YGG AN L DGYS GTGLA EI+GTFVL+Y
Sbjct: 124 RALLYIIAQCLGAICGVGFVKAFQSSYYTRYGGGANSLADGYSTGTGLAAEIIGTFVLVY 183

Query: 185 TVFSATDPKRNARDSYVPVLAPLPIGFTVFMVHLATIPITGTGINPARSFGAAVIINDKE 244
           TVFSATDPKR+ARDS+VPVLAPLPIGF VFMVHLATIPITGTGINPARSFGAAVI N  +
Sbjct: 184 TVFSATDPKRSARDSHVPVLAPLPIGFAVFMVHLATIPITGTGINPARSFGAAVIYNKSK 243

Query: 245 IWKDHWIFWVGPLIGATIATMYYQAVAIPSESSS 279
            W DHWIFWVGP IGA IA  Y+Q V   S S S
Sbjct: 244 PWDDHWIFWVGPFIGAAIAAFYHQFVLRASGSKS 277

BLAST of CmaCh06G003750 vs. ExPASy Swiss-Prot
Match: Q9ATM7 (Aquaporin PIP2-3 OS=Zea mays OX=4577 GN=PIP2-3 PE=2 SV=1)

HSP 1 Score: 399.1 bits (1024), Expect = 6.1e-110
Identity = 200/274 (72.99%), Postives = 218/274 (79.56%), Query Frame = 0

Query: 1   MANNDIEVG--SRGSLPRKDYFEPLPAPMIDMEELAKWSFYRAIIAEFVATLLFLYVVVL 60
           MA  DIE      G    KDY +P PAP+ID +EL KWS YRA+IAEF+ATLLFLY+ V 
Sbjct: 1   MAKQDIEASGPEAGEFSAKDYTDPPPAPLIDADELTKWSLYRAVIAEFIATLLFLYITVA 60

Query: 61  TVIGNKTQTDPIIGG--NLCGGVGTLGIAWVFGGMIFVLVYCTAGISGGHINPAVTFGMF 120
           TVIG K QTD    G    CGGVG LGIAW FGGMIF+LVYCTAGISGGHINPAVTFG+F
Sbjct: 61  TVIGYKHQTDAAASGPDAACGGVGILGIAWAFGGMIFILVYCTAGISGGHINPAVTFGLF 120

Query: 121 LGRKISLIRALLYIIAQCLGALCGCALVKSLQRDRYNHYGGAANQLVDGYSKGTGLAVEI 180
           L RK+SL+RALLYIIAQCLGA+CG  LVK  Q   Y  YGG AN+L DGYSKGTGLA EI
Sbjct: 121 LARKVSLVRALLYIIAQCLGAICGVGLVKGFQSAYYVRYGGGANELSDGYSKGTGLAAEI 180

Query: 181 VGTFVLLYTVFSATDPKRNARDSYVPVLAPLPIGFTVFMVHLATIPITGTGINPARSFGA 240
           +GTFVL+YTVFSATDPKR+ARDS+VPVLAPLPIGF VFMVHLATIPITGTGINPARS GA
Sbjct: 181 IGTFVLVYTVFSATDPKRSARDSHVPVLAPLPIGFAVFMVHLATIPITGTGINPARSLGA 240

Query: 241 AVIINDKEIWKDHWIFWVGPLIGATIATMYYQAV 271
           AVI N  + W D WIFWVGPLIGA IA  Y+Q V
Sbjct: 241 AVIYNKDKAWDDQWIFWVGPLIGAAIAAAYHQYV 274

BLAST of CmaCh06G003750 vs. ExPASy Swiss-Prot
Match: P30302 (Aquaporin PIP2-3 OS=Arabidopsis thaliana OX=3702 GN=PIP2-3 PE=1 SV=1)

HSP 1 Score: 398.7 bits (1023), Expect = 7.9e-110
Identity = 196/262 (74.81%), Postives = 215/262 (82.06%), Query Frame = 0

Query: 17  KDYFEPLPAPMIDMEELAKWSFYRAIIAEFVATLLFLYVVVLTVIGNKTQTDPIIGGNLC 76
           +DY +P P P  D EEL KWS YRA+IAEFVATLLFLYV VLTVIG K Q+D   GG  C
Sbjct: 14  RDYEDPPPTPFFDAEELTKWSLYRAVIAEFVATLLFLYVTVLTVIGYKIQSDTKAGGVDC 73

Query: 77  GGVGTLGIAWVFGGMIFVLVYCTAGISGGHINPAVTFGMFLGRKISLIRALLYIIAQCLG 136
           GGVG LGIAW FGGMIF+LVYCTAGISGGHINPAVTFG+FL RK+SLIRA+LY++AQCLG
Sbjct: 74  GGVGILGIAWAFGGMIFILVYCTAGISGGHINPAVTFGLFLARKVSLIRAVLYMVAQCLG 133

Query: 137 ALCGCALVKSLQRDRYNHYGGAANQLVDGYSKGTGLAVEIVGTFVLLYTVFSATDPKRNA 196
           A+CG   VK+ Q   Y +YGG AN L DGY+ GTGLA EI+GTFVL+YTVFSATDPKRNA
Sbjct: 134 AICGVGFVKAFQSSHYVNYGGGANFLADGYNTGTGLAAEIIGTFVLVYTVFSATDPKRNA 193

Query: 197 RDSYVPVLAPLPIGFTVFMVHLATIPITGTGINPARSFGAAVIINDKEIWKDHWIFWVGP 256
           RDS+VPVLAPLPIGF VFMVHLATIPITGTGINPARSFGAAVI N  + W DHWIFWVGP
Sbjct: 194 RDSHVPVLAPLPIGFAVFMVHLATIPITGTGINPARSFGAAVIFNKSKPWDDHWIFWVGP 253

Query: 257 LIGATIATMYYQAVAIPSESSS 279
            IGATIA  Y+Q V   S S S
Sbjct: 254 FIGATIAAFYHQFVLRASGSKS 275

BLAST of CmaCh06G003750 vs. ExPASy Swiss-Prot
Match: P43287 (Aquaporin PIP2-2 OS=Arabidopsis thaliana OX=3702 GN=PIP2-2 PE=1 SV=2)

HSP 1 Score: 396.4 bits (1017), Expect = 3.9e-109
Identity = 193/262 (73.66%), Postives = 214/262 (81.68%), Query Frame = 0

Query: 17  KDYFEPLPAPMIDMEELAKWSFYRAIIAEFVATLLFLYVVVLTVIGNKTQTDPIIGGNLC 76
           +DY +P P P  D +EL KWS YRA+IAEFVATLLFLY+ VLTVIG K Q+D   GG  C
Sbjct: 14  RDYEDPPPTPFFDADELTKWSLYRAVIAEFVATLLFLYITVLTVIGYKIQSDTKAGGVDC 73

Query: 77  GGVGTLGIAWVFGGMIFVLVYCTAGISGGHINPAVTFGMFLGRKISLIRALLYIIAQCLG 136
           GGVG LGIAW FGGMIF+LVYCTAGISGGHINPAVTFG+FL RK+SLIRA+LY++AQCLG
Sbjct: 74  GGVGILGIAWAFGGMIFILVYCTAGISGGHINPAVTFGLFLARKVSLIRAVLYMVAQCLG 133

Query: 137 ALCGCALVKSLQRDRYNHYGGAANQLVDGYSKGTGLAVEIVGTFVLLYTVFSATDPKRNA 196
           A+CG   VK+ Q   Y+ YGG AN L DGY+ GTGLA EI+GTFVL+YTVFSATDPKRNA
Sbjct: 134 AICGVGFVKAFQSSYYDRYGGGANSLADGYNTGTGLAAEIIGTFVLVYTVFSATDPKRNA 193

Query: 197 RDSYVPVLAPLPIGFTVFMVHLATIPITGTGINPARSFGAAVIINDKEIWKDHWIFWVGP 256
           RDS+VPVLAPLPIGF VFMVHLATIPITGTGINPARSFGAAVI N  + W DHWIFWVGP
Sbjct: 194 RDSHVPVLAPLPIGFAVFMVHLATIPITGTGINPARSFGAAVIYNKSKPWDDHWIFWVGP 253

Query: 257 LIGATIATMYYQAVAIPSESSS 279
            IGA IA  Y+Q V   S S S
Sbjct: 254 FIGAAIAAFYHQFVLRASGSKS 275

BLAST of CmaCh06G003750 vs. ExPASy TrEMBL
Match: A0A6J1KYL1 (probable aquaporin PIP2-4 OS=Cucurbita maxima OX=3661 GN=LOC111498744 PE=3 SV=1)

HSP 1 Score: 542.3 bits (1396), Expect = 1.6e-150
Identity = 269/270 (99.63%), Postives = 269/270 (99.63%), Query Frame = 0

Query: 1   MANNDIEVGSRGSLPRKDYFEPLPAPMIDMEELAKWSFYRAIIAEFVATLLFLYVVVLTV 60
           MANNDIEVGSRGSLPRKDYFEPLPAPMIDMEELAKWSFYRAIIAEFVATLLFLYVVVLTV
Sbjct: 1   MANNDIEVGSRGSLPRKDYFEPLPAPMIDMEELAKWSFYRAIIAEFVATLLFLYVVVLTV 60

Query: 61  IGNKTQTDPIIGGNLCGGVGTLGIAWVFGGMIFVLVYCTAGISGGHINPAVTFGMFLGRK 120
           IGNKTQTDPIIGGNLCGGVGTLGIAWVFGGMIFVLVYCTAGISGGHINPAVTFGMFLGRK
Sbjct: 61  IGNKTQTDPIIGGNLCGGVGTLGIAWVFGGMIFVLVYCTAGISGGHINPAVTFGMFLGRK 120

Query: 121 ISLIRALLYIIAQCLGALCGCALVKSLQRDRYNHYGGAANQLVDGYSKGTGLAVEIVGTF 180
           ISLIRALLYIIAQCLGALCGCALVKSLQRDRYNHYGGAANQLVDGYSKGTGLAVEIVGTF
Sbjct: 121 ISLIRALLYIIAQCLGALCGCALVKSLQRDRYNHYGGAANQLVDGYSKGTGLAVEIVGTF 180

Query: 181 VLLYTVFSATDPKRNARDSYVPVLAPLPIGFTVFMVHLATIPITGTGINPARSFGAAVII 240
           VLLYTVFSATDPKRNARDSYVPVLAPLPIGFTVFMVHLATIPITGTGINPARSFGAAVII
Sbjct: 181 VLLYTVFSATDPKRNARDSYVPVLAPLPIGFTVFMVHLATIPITGTGINPARSFGAAVII 240

Query: 241 NDKEIWKDHWIFWVGPLIGATIATMYYQAV 271
           NDKEIWKDHWIFWVGPLIGATIATMYYQ V
Sbjct: 241 NDKEIWKDHWIFWVGPLIGATIATMYYQYV 270

BLAST of CmaCh06G003750 vs. ExPASy TrEMBL
Match: A0A6J1H847 (probable aquaporin PIP2-4 OS=Cucurbita moschata OX=3662 GN=LOC111461325 PE=3 SV=1)

HSP 1 Score: 535.8 bits (1379), Expect = 1.5e-148
Identity = 264/270 (97.78%), Postives = 267/270 (98.89%), Query Frame = 0

Query: 1   MANNDIEVGSRGSLPRKDYFEPLPAPMIDMEELAKWSFYRAIIAEFVATLLFLYVVVLTV 60
           MANNDIEVGSRGSLPRKDYFEPLPAPMIDMEELAKWSFYRAIIAEFVATLLFLYV VLTV
Sbjct: 1   MANNDIEVGSRGSLPRKDYFEPLPAPMIDMEELAKWSFYRAIIAEFVATLLFLYVAVLTV 60

Query: 61  IGNKTQTDPIIGGNLCGGVGTLGIAWVFGGMIFVLVYCTAGISGGHINPAVTFGMFLGRK 120
           IGNKTQTDP+ GGNLCGGVGTLGIAWVFGGMIFVLVYCTAGISGGHINPAVTFGMFLGRK
Sbjct: 61  IGNKTQTDPLNGGNLCGGVGTLGIAWVFGGMIFVLVYCTAGISGGHINPAVTFGMFLGRK 120

Query: 121 ISLIRALLYIIAQCLGALCGCALVKSLQRDRYNHYGGAANQLVDGYSKGTGLAVEIVGTF 180
           ISLIRALLYIIAQCLGALCGCALVKSLQRDRYNHYGGAANQLVDGYSKGTGLAVEI+GTF
Sbjct: 121 ISLIRALLYIIAQCLGALCGCALVKSLQRDRYNHYGGAANQLVDGYSKGTGLAVEIIGTF 180

Query: 181 VLLYTVFSATDPKRNARDSYVPVLAPLPIGFTVFMVHLATIPITGTGINPARSFGAAVII 240
           VLLYTVFSATDPKRNARDSYVPVLAPLPIGFTVFMVHLATIPITGTGINPARSFGAAVII
Sbjct: 181 VLLYTVFSATDPKRNARDSYVPVLAPLPIGFTVFMVHLATIPITGTGINPARSFGAAVII 240

Query: 241 NDKEIWKDHWIFWVGPLIGATIATMYYQAV 271
           NDK+IWKDHWIFWVGPLIGATIATMYYQ V
Sbjct: 241 NDKQIWKDHWIFWVGPLIGATIATMYYQYV 270

BLAST of CmaCh06G003750 vs. ExPASy TrEMBL
Match: A0A5A7TKK0 (Putative aquaporin PIP2-4 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G002520 PE=3 SV=1)

HSP 1 Score: 491.1 bits (1263), Expect = 4.3e-135
Identity = 236/270 (87.41%), Postives = 256/270 (94.81%), Query Frame = 0

Query: 1   MANNDIEVGSRGSLPRKDYFEPLPAPMIDMEELAKWSFYRAIIAEFVATLLFLYVVVLTV 60
           MA++DIE+G RG+  RKDYF+PLPAPMIDMEE AKWSFYRAIIAEFVATLLFLYVVVLTV
Sbjct: 1   MASSDIEIGGRGAASRKDYFDPLPAPMIDMEEFAKWSFYRAIIAEFVATLLFLYVVVLTV 60

Query: 61  IGNKTQTDPIIGGNLCGGVGTLGIAWVFGGMIFVLVYCTAGISGGHINPAVTFGMFLGRK 120
           IGN +QTDP+ GGN+C GVG +GI+WVFGGMIFVLVYCTAGISGGHINPAVTFGMFL RK
Sbjct: 61  IGNSSQTDPLNGGNVCAGVGPVGISWVFGGMIFVLVYCTAGISGGHINPAVTFGMFLTRK 120

Query: 121 ISLIRALLYIIAQCLGALCGCALVKSLQRDRYNHYGGAANQLVDGYSKGTGLAVEIVGTF 180
           ISL+RALLYIIAQC+GALCGCALVK+LQRDRYNHYGG ANQLVDGYS+GTGLAVEIVGTF
Sbjct: 121 ISLVRALLYIIAQCIGALCGCALVKTLQRDRYNHYGGGANQLVDGYSRGTGLAVEIVGTF 180

Query: 181 VLLYTVFSATDPKRNARDSYVPVLAPLPIGFTVFMVHLATIPITGTGINPARSFGAAVII 240
           VLLYTVFSATDPKRNARDS+VPVLAPLPIGF VFMVHLATIP+TGTGINPARSFGAAVII
Sbjct: 181 VLLYTVFSATDPKRNARDSHVPVLAPLPIGFAVFMVHLATIPVTGTGINPARSFGAAVII 240

Query: 241 NDKEIWKDHWIFWVGPLIGATIATMYYQAV 271
           N+ ++WKDHWIFWVGPLIG+TIA MYYQ V
Sbjct: 241 NNHKVWKDHWIFWVGPLIGSTIAAMYYQYV 270

BLAST of CmaCh06G003750 vs. ExPASy TrEMBL
Match: A0A1S3B699 (probable aquaporin PIP2-4 OS=Cucumis melo OX=3656 GN=LOC103486477 PE=3 SV=1)

HSP 1 Score: 491.1 bits (1263), Expect = 4.3e-135
Identity = 236/270 (87.41%), Postives = 256/270 (94.81%), Query Frame = 0

Query: 1   MANNDIEVGSRGSLPRKDYFEPLPAPMIDMEELAKWSFYRAIIAEFVATLLFLYVVVLTV 60
           MA++DIE+G RG+  RKDYF+PLPAPMIDMEE AKWSFYRAIIAEFVATLLFLYVVVLTV
Sbjct: 1   MASSDIEIGGRGAASRKDYFDPLPAPMIDMEEFAKWSFYRAIIAEFVATLLFLYVVVLTV 60

Query: 61  IGNKTQTDPIIGGNLCGGVGTLGIAWVFGGMIFVLVYCTAGISGGHINPAVTFGMFLGRK 120
           IGN +QTDP+ GGN+C GVG +GI+WVFGGMIFVLVYCTAGISGGHINPAVTFGMFL RK
Sbjct: 61  IGNSSQTDPLNGGNVCAGVGPVGISWVFGGMIFVLVYCTAGISGGHINPAVTFGMFLTRK 120

Query: 121 ISLIRALLYIIAQCLGALCGCALVKSLQRDRYNHYGGAANQLVDGYSKGTGLAVEIVGTF 180
           ISL+RALLYIIAQC+GALCGCALVK+LQRDRYNHYGG ANQLVDGYS+GTGLAVEIVGTF
Sbjct: 121 ISLVRALLYIIAQCIGALCGCALVKTLQRDRYNHYGGGANQLVDGYSRGTGLAVEIVGTF 180

Query: 181 VLLYTVFSATDPKRNARDSYVPVLAPLPIGFTVFMVHLATIPITGTGINPARSFGAAVII 240
           VLLYTVFSATDPKRNARDS+VPVLAPLPIGF VFMVHLATIP+TGTGINPARSFGAAVII
Sbjct: 181 VLLYTVFSATDPKRNARDSHVPVLAPLPIGFAVFMVHLATIPVTGTGINPARSFGAAVII 240

Query: 241 NDKEIWKDHWIFWVGPLIGATIATMYYQAV 271
           N+ ++WKDHWIFWVGPLIG+TIA MYYQ V
Sbjct: 241 NNHKVWKDHWIFWVGPLIGSTIAAMYYQYV 270

BLAST of CmaCh06G003750 vs. ExPASy TrEMBL
Match: A0A0A0LDZ7 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G739030 PE=3 SV=1)

HSP 1 Score: 482.3 bits (1240), Expect = 2.0e-132
Identity = 231/270 (85.56%), Postives = 252/270 (93.33%), Query Frame = 0

Query: 1   MANNDIEVGSRGSLPRKDYFEPLPAPMIDMEELAKWSFYRAIIAEFVATLLFLYVVVLTV 60
           MA +DIE+G RG+  RKDYF+PLPAPMIDMEEL KWSFYRAIIAEFVATLLFLYVVVLTV
Sbjct: 1   MATSDIEIGGRGAASRKDYFDPLPAPMIDMEELGKWSFYRAIIAEFVATLLFLYVVVLTV 60

Query: 61  IGNKTQTDPIIGGNLCGGVGTLGIAWVFGGMIFVLVYCTAGISGGHINPAVTFGMFLGRK 120
           IGN +Q DPI GGN+C GVG +GI+WVFGGMIFVLVYCTAGISGGHINPAVTFGMFL RK
Sbjct: 61  IGNNSQMDPINGGNVCAGVGPVGISWVFGGMIFVLVYCTAGISGGHINPAVTFGMFLTRK 120

Query: 121 ISLIRALLYIIAQCLGALCGCALVKSLQRDRYNHYGGAANQLVDGYSKGTGLAVEIVGTF 180
           ISL+RALLYIIAQC+GALCGCALVK+LQRD YNHYGG ANQLVDGYS+GTGLAVEI+GTF
Sbjct: 121 ISLVRALLYIIAQCIGALCGCALVKTLQRDHYNHYGGGANQLVDGYSRGTGLAVEIMGTF 180

Query: 181 VLLYTVFSATDPKRNARDSYVPVLAPLPIGFTVFMVHLATIPITGTGINPARSFGAAVII 240
           +LLYTVFSATDPKRNARDS+VPVLAPLPIGF VF+VHLATIP+TGTGINPARSFG AVII
Sbjct: 181 ILLYTVFSATDPKRNARDSHVPVLAPLPIGFAVFIVHLATIPVTGTGINPARSFGPAVII 240

Query: 241 NDKEIWKDHWIFWVGPLIGATIATMYYQAV 271
           N+ ++WKDHWIFWVGPLIG+TIA MYYQ V
Sbjct: 241 NNHKVWKDHWIFWVGPLIGSTIAAMYYQYV 270

BLAST of CmaCh06G003750 vs. NCBI nr
Match: KAG6596471.1 (putative aquaporin PIP2-4, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 713.0 bits (1839), Expect = 1.4e-201
Identity = 374/405 (92.35%), Postives = 382/405 (94.32%), Query Frame = 0

Query: 1   MANNDIEVGSRGSLPRKDYFEPLPAPMIDMEELAKWSFYRAIIAEFVATLLFLYVVVLTV 60
           MANNDIEVGSRGSLPRKDYFEPLPAPMIDMEELAKWSFYRAIIAEFVATLLFLYV VLTV
Sbjct: 1   MANNDIEVGSRGSLPRKDYFEPLPAPMIDMEELAKWSFYRAIIAEFVATLLFLYVAVLTV 60

Query: 61  IGNKTQTDPIIGGNLCGGVGTLGIAWVFGGMIFVLVYCTAGISGGHINPAVTFGMFLGRK 120
           IGNKTQTDP+ GGNLCGGVGTLGIAWVFGGMIFVLVYCTAGISGGHINPAVTFGMFLGRK
Sbjct: 61  IGNKTQTDPLNGGNLCGGVGTLGIAWVFGGMIFVLVYCTAGISGGHINPAVTFGMFLGRK 120

Query: 121 ISLIRALLYIIAQCLGALCGCALVKSLQRDRYNHYGGAANQLVDGYSKGTGLAVEIVGTF 180
           ISLIRALLYIIAQCLGALCGCALVKSLQRDRYNHYGGAANQLVDGYSKGTGLAVEI+GTF
Sbjct: 121 ISLIRALLYIIAQCLGALCGCALVKSLQRDRYNHYGGAANQLVDGYSKGTGLAVEIIGTF 180

Query: 181 VLLYTVFSATDPKRNARDSYVPVLAPLPIGFTVFMVHLATIPITGTGINPARSFGAAVII 240
           VLLYTVFSATDPKRNARDSYVPVLAPLPIGFTVFMVHLATIPITGTGINPARSFGAAVII
Sbjct: 181 VLLYTVFSATDPKRNARDSYVPVLAPLPIGFTVFMVHLATIPITGTGINPARSFGAAVII 240

Query: 241 NDKEIWKDHWI------FWVGPLIGATIATMYYQAVAIPSESSSFFAPGNLYLQENMATR 300
           NDK+IWKDH I      F++ P+            +AIPSESSSFFAPGNL+LQENMATR
Sbjct: 241 NDKQIWKDHEIPTPSRTFFLRPI-----------GLAIPSESSSFFAPGNLHLQENMATR 300

Query: 301 GQGGIQQLLAAEQEAQHIVNAARNAKLARLKQAKEEAEKEIAAYRAQVEAEFQRKLSESS 360
           GQGGIQQLLAAEQEAQHIVNAARNAKLARLKQAKEEAEKEIAAYRAQVEAEFQRKLSESS
Sbjct: 301 GQGGIQQLLAAEQEAQHIVNAARNAKLARLKQAKEEAEKEIAAYRAQVEAEFQRKLSESS 360

Query: 361 GDSGANVKRLEVETDAKIAHLKEECARISYDVVDMLLKHVTTVKN 400
           GDSGANVKRLEVETDAKIAHLKEE ARISYDVVDMLLKHVTTVKN
Sbjct: 361 GDSGANVKRLEVETDAKIAHLKEESARISYDVVDMLLKHVTTVKN 394

BLAST of CmaCh06G003750 vs. NCBI nr
Match: XP_023005870.1 (probable aquaporin PIP2-4 [Cucurbita maxima])

HSP 1 Score: 542.3 bits (1396), Expect = 3.4e-150
Identity = 269/270 (99.63%), Postives = 269/270 (99.63%), Query Frame = 0

Query: 1   MANNDIEVGSRGSLPRKDYFEPLPAPMIDMEELAKWSFYRAIIAEFVATLLFLYVVVLTV 60
           MANNDIEVGSRGSLPRKDYFEPLPAPMIDMEELAKWSFYRAIIAEFVATLLFLYVVVLTV
Sbjct: 1   MANNDIEVGSRGSLPRKDYFEPLPAPMIDMEELAKWSFYRAIIAEFVATLLFLYVVVLTV 60

Query: 61  IGNKTQTDPIIGGNLCGGVGTLGIAWVFGGMIFVLVYCTAGISGGHINPAVTFGMFLGRK 120
           IGNKTQTDPIIGGNLCGGVGTLGIAWVFGGMIFVLVYCTAGISGGHINPAVTFGMFLGRK
Sbjct: 61  IGNKTQTDPIIGGNLCGGVGTLGIAWVFGGMIFVLVYCTAGISGGHINPAVTFGMFLGRK 120

Query: 121 ISLIRALLYIIAQCLGALCGCALVKSLQRDRYNHYGGAANQLVDGYSKGTGLAVEIVGTF 180
           ISLIRALLYIIAQCLGALCGCALVKSLQRDRYNHYGGAANQLVDGYSKGTGLAVEIVGTF
Sbjct: 121 ISLIRALLYIIAQCLGALCGCALVKSLQRDRYNHYGGAANQLVDGYSKGTGLAVEIVGTF 180

Query: 181 VLLYTVFSATDPKRNARDSYVPVLAPLPIGFTVFMVHLATIPITGTGINPARSFGAAVII 240
           VLLYTVFSATDPKRNARDSYVPVLAPLPIGFTVFMVHLATIPITGTGINPARSFGAAVII
Sbjct: 181 VLLYTVFSATDPKRNARDSYVPVLAPLPIGFTVFMVHLATIPITGTGINPARSFGAAVII 240

Query: 241 NDKEIWKDHWIFWVGPLIGATIATMYYQAV 271
           NDKEIWKDHWIFWVGPLIGATIATMYYQ V
Sbjct: 241 NDKEIWKDHWIFWVGPLIGATIATMYYQYV 270

BLAST of CmaCh06G003750 vs. NCBI nr
Match: XP_023540178.1 (probable aquaporin PIP2-4 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 536.2 bits (1380), Expect = 2.4e-148
Identity = 265/270 (98.15%), Postives = 267/270 (98.89%), Query Frame = 0

Query: 1   MANNDIEVGSRGSLPRKDYFEPLPAPMIDMEELAKWSFYRAIIAEFVATLLFLYVVVLTV 60
           MANNDIEVGSRGSLPRKDYFEPLPAPMIDMEELAKWSFYRAIIAEFVATLLFLYV VLTV
Sbjct: 1   MANNDIEVGSRGSLPRKDYFEPLPAPMIDMEELAKWSFYRAIIAEFVATLLFLYVTVLTV 60

Query: 61  IGNKTQTDPIIGGNLCGGVGTLGIAWVFGGMIFVLVYCTAGISGGHINPAVTFGMFLGRK 120
           IGNKTQTDP+ GGNLCGGVGTLGIAWVFGGMIFVLVYCTAGISGGHINPAVTFGMFLGRK
Sbjct: 61  IGNKTQTDPLNGGNLCGGVGTLGIAWVFGGMIFVLVYCTAGISGGHINPAVTFGMFLGRK 120

Query: 121 ISLIRALLYIIAQCLGALCGCALVKSLQRDRYNHYGGAANQLVDGYSKGTGLAVEIVGTF 180
           ISLIRALLYIIAQCLGALCGCALVKSLQRDRYNHYGGAANQLVDGYSKGTGLAVEIVGTF
Sbjct: 121 ISLIRALLYIIAQCLGALCGCALVKSLQRDRYNHYGGAANQLVDGYSKGTGLAVEIVGTF 180

Query: 181 VLLYTVFSATDPKRNARDSYVPVLAPLPIGFTVFMVHLATIPITGTGINPARSFGAAVII 240
           VLLYTVFSATDPKRNARDSYVPVLAPLPIGFTVFMVHLATIPITGTGINPARSFGAAVII
Sbjct: 181 VLLYTVFSATDPKRNARDSYVPVLAPLPIGFTVFMVHLATIPITGTGINPARSFGAAVII 240

Query: 241 NDKEIWKDHWIFWVGPLIGATIATMYYQAV 271
           NDK+IWKDHWIFWVGPLIGATIATMYYQ V
Sbjct: 241 NDKQIWKDHWIFWVGPLIGATIATMYYQYV 270

BLAST of CmaCh06G003750 vs. NCBI nr
Match: XP_022960621.1 (probable aquaporin PIP2-4 [Cucurbita moschata])

HSP 1 Score: 535.8 bits (1379), Expect = 3.2e-148
Identity = 264/270 (97.78%), Postives = 267/270 (98.89%), Query Frame = 0

Query: 1   MANNDIEVGSRGSLPRKDYFEPLPAPMIDMEELAKWSFYRAIIAEFVATLLFLYVVVLTV 60
           MANNDIEVGSRGSLPRKDYFEPLPAPMIDMEELAKWSFYRAIIAEFVATLLFLYV VLTV
Sbjct: 1   MANNDIEVGSRGSLPRKDYFEPLPAPMIDMEELAKWSFYRAIIAEFVATLLFLYVAVLTV 60

Query: 61  IGNKTQTDPIIGGNLCGGVGTLGIAWVFGGMIFVLVYCTAGISGGHINPAVTFGMFLGRK 120
           IGNKTQTDP+ GGNLCGGVGTLGIAWVFGGMIFVLVYCTAGISGGHINPAVTFGMFLGRK
Sbjct: 61  IGNKTQTDPLNGGNLCGGVGTLGIAWVFGGMIFVLVYCTAGISGGHINPAVTFGMFLGRK 120

Query: 121 ISLIRALLYIIAQCLGALCGCALVKSLQRDRYNHYGGAANQLVDGYSKGTGLAVEIVGTF 180
           ISLIRALLYIIAQCLGALCGCALVKSLQRDRYNHYGGAANQLVDGYSKGTGLAVEI+GTF
Sbjct: 121 ISLIRALLYIIAQCLGALCGCALVKSLQRDRYNHYGGAANQLVDGYSKGTGLAVEIIGTF 180

Query: 181 VLLYTVFSATDPKRNARDSYVPVLAPLPIGFTVFMVHLATIPITGTGINPARSFGAAVII 240
           VLLYTVFSATDPKRNARDSYVPVLAPLPIGFTVFMVHLATIPITGTGINPARSFGAAVII
Sbjct: 181 VLLYTVFSATDPKRNARDSYVPVLAPLPIGFTVFMVHLATIPITGTGINPARSFGAAVII 240

Query: 241 NDKEIWKDHWIFWVGPLIGATIATMYYQAV 271
           NDK+IWKDHWIFWVGPLIGATIATMYYQ V
Sbjct: 241 NDKQIWKDHWIFWVGPLIGATIATMYYQYV 270

BLAST of CmaCh06G003750 vs. NCBI nr
Match: NP_001380714.1 (aquaporin PIP2-8 [Cucumis melo] >KAA0044013.1 putative aquaporin PIP2-4 [Cucumis melo var. makuwa] >TYK25127.1 putative aquaporin PIP2-4 [Cucumis melo var. makuwa])

HSP 1 Score: 491.1 bits (1263), Expect = 9.0e-135
Identity = 236/270 (87.41%), Postives = 256/270 (94.81%), Query Frame = 0

Query: 1   MANNDIEVGSRGSLPRKDYFEPLPAPMIDMEELAKWSFYRAIIAEFVATLLFLYVVVLTV 60
           MA++DIE+G RG+  RKDYF+PLPAPMIDMEE AKWSFYRAIIAEFVATLLFLYVVVLTV
Sbjct: 1   MASSDIEIGGRGAASRKDYFDPLPAPMIDMEEFAKWSFYRAIIAEFVATLLFLYVVVLTV 60

Query: 61  IGNKTQTDPIIGGNLCGGVGTLGIAWVFGGMIFVLVYCTAGISGGHINPAVTFGMFLGRK 120
           IGN +QTDP+ GGN+C GVG +GI+WVFGGMIFVLVYCTAGISGGHINPAVTFGMFL RK
Sbjct: 61  IGNSSQTDPLNGGNVCAGVGPVGISWVFGGMIFVLVYCTAGISGGHINPAVTFGMFLTRK 120

Query: 121 ISLIRALLYIIAQCLGALCGCALVKSLQRDRYNHYGGAANQLVDGYSKGTGLAVEIVGTF 180
           ISL+RALLYIIAQC+GALCGCALVK+LQRDRYNHYGG ANQLVDGYS+GTGLAVEIVGTF
Sbjct: 121 ISLVRALLYIIAQCIGALCGCALVKTLQRDRYNHYGGGANQLVDGYSRGTGLAVEIVGTF 180

Query: 181 VLLYTVFSATDPKRNARDSYVPVLAPLPIGFTVFMVHLATIPITGTGINPARSFGAAVII 240
           VLLYTVFSATDPKRNARDS+VPVLAPLPIGF VFMVHLATIP+TGTGINPARSFGAAVII
Sbjct: 181 VLLYTVFSATDPKRNARDSHVPVLAPLPIGFAVFMVHLATIPVTGTGINPARSFGAAVII 240

Query: 241 NDKEIWKDHWIFWVGPLIGATIATMYYQAV 271
           N+ ++WKDHWIFWVGPLIG+TIA MYYQ V
Sbjct: 241 NNHKVWKDHWIFWVGPLIGSTIAAMYYQYV 270

BLAST of CmaCh06G003750 vs. TAIR 10
Match: AT5G60660.1 (plasma membrane intrinsic protein 2;4 )

HSP 1 Score: 404.1 bits (1037), Expect = 1.3e-112
Identity = 193/266 (72.56%), Postives = 217/266 (81.58%), Query Frame = 0

Query: 5   DIEVGSRGSLPRKDYFEPLPAPMIDMEELAKWSFYRAIIAEFVATLLFLYVVVLTVIGNK 64
           D++V   G    +DY +P PAP  DMEEL KW  YRA+IAEFVATLLFLYV +LTVIG K
Sbjct: 4   DLDVNESGPPAARDYKDPPPAPFFDMEELRKWPLYRAVIAEFVATLLFLYVSILTVIGYK 63

Query: 65  TQTDPIIGGNLCGGVGTLGIAWVFGGMIFVLVYCTAGISGGHINPAVTFGMFLGRKISLI 124
            QTD   GG  CGGVG LGIAW FGGMIFVLVYCTAGISGGHINPAVT G+FL RK+SL+
Sbjct: 64  AQTDATAGGVDCGGVGILGIAWAFGGMIFVLVYCTAGISGGHINPAVTVGLFLARKVSLV 123

Query: 125 RALLYIIAQCLGALCGCALVKSLQRDRYNHYGGAANQLVDGYSKGTGLAVEIVGTFVLLY 184
           R +LYI+AQCLGA+CGC  VK+ Q   Y  YGG AN+L DGY+KGTGL  EI+GTFVL+Y
Sbjct: 124 RTVLYIVAQCLGAICGCGFVKAFQSSYYTRYGGGANELADGYNKGTGLGAEIIGTFVLVY 183

Query: 185 TVFSATDPKRNARDSYVPVLAPLPIGFTVFMVHLATIPITGTGINPARSFGAAVIINDKE 244
           TVFSATDPKRNARDS+VPVLAPLPIGF VFMVHLATIPITGTGINPARSFGAAVI N+++
Sbjct: 184 TVFSATDPKRNARDSHVPVLAPLPIGFAVFMVHLATIPITGTGINPARSFGAAVIYNNEK 243

Query: 245 IWKDHWIFWVGPLIGATIATMYYQAV 271
            W D WIFWVGP+IGA  A  Y+Q +
Sbjct: 244 AWDDQWIFWVGPMIGAAAAAFYHQFI 269

BLAST of CmaCh06G003750 vs. TAIR 10
Match: AT3G53420.1 (plasma membrane intrinsic protein 2A )

HSP 1 Score: 400.6 bits (1028), Expect = 1.5e-111
Identity = 200/274 (72.99%), Postives = 217/274 (79.20%), Query Frame = 0

Query: 5   DIEVGSRGSLPRKDYFEPLPAPMIDMEELAKWSFYRAIIAEFVATLLFLYVVVLTVIGNK 64
           D+E         +DY +P PAP ID  EL KWSFYRA+IAEFVATLLFLY+ VLTVIG K
Sbjct: 4   DVEAVPGEGFQTRDYQDPPPAPFIDGAELKKWSFYRAVIAEFVATLLFLYITVLTVIGYK 63

Query: 65  TQTDPIIGGNLCGGVGTLGIAWVFGGMIFVLVYCTAGISGGHINPAVTFGMFLGRKISLI 124
            Q+D   GG  CGGVG LGIAW FGGMIF+LVYCTAGISGGHINPAVTFG+FL RK+SL 
Sbjct: 64  IQSDTDAGGVDCGGVGILGIAWAFGGMIFILVYCTAGISGGHINPAVTFGLFLARKVSLP 123

Query: 125 RALLYIIAQCLGALCGCALVKSLQRDRYNHYGGAANQLVDGYSKGTGLAVEIVGTFVLLY 184
           RALLYIIAQCLGA+CG   VK+ Q   Y  YGG AN L DGYS GTGLA EI+GTFVL+Y
Sbjct: 124 RALLYIIAQCLGAICGVGFVKAFQSSYYTRYGGGANSLADGYSTGTGLAAEIIGTFVLVY 183

Query: 185 TVFSATDPKRNARDSYVPVLAPLPIGFTVFMVHLATIPITGTGINPARSFGAAVIINDKE 244
           TVFSATDPKR+ARDS+VPVLAPLPIGF VFMVHLATIPITGTGINPARSFGAAVI N  +
Sbjct: 184 TVFSATDPKRSARDSHVPVLAPLPIGFAVFMVHLATIPITGTGINPARSFGAAVIYNKSK 243

Query: 245 IWKDHWIFWVGPLIGATIATMYYQAVAIPSESSS 279
            W DHWIFWVGP IGA IA  Y+Q V   S S S
Sbjct: 244 PWDDHWIFWVGPFIGAAIAAFYHQFVLRASGSKS 277

BLAST of CmaCh06G003750 vs. TAIR 10
Match: AT3G53420.2 (plasma membrane intrinsic protein 2A )

HSP 1 Score: 400.6 bits (1028), Expect = 1.5e-111
Identity = 200/274 (72.99%), Postives = 217/274 (79.20%), Query Frame = 0

Query: 5   DIEVGSRGSLPRKDYFEPLPAPMIDMEELAKWSFYRAIIAEFVATLLFLYVVVLTVIGNK 64
           D+E         +DY +P PAP ID  EL KWSFYRA+IAEFVATLLFLY+ VLTVIG K
Sbjct: 4   DVEAVPGEGFQTRDYQDPPPAPFIDGAELKKWSFYRAVIAEFVATLLFLYITVLTVIGYK 63

Query: 65  TQTDPIIGGNLCGGVGTLGIAWVFGGMIFVLVYCTAGISGGHINPAVTFGMFLGRKISLI 124
            Q+D   GG  CGGVG LGIAW FGGMIF+LVYCTAGISGGHINPAVTFG+FL RK+SL 
Sbjct: 64  IQSDTDAGGVDCGGVGILGIAWAFGGMIFILVYCTAGISGGHINPAVTFGLFLARKVSLP 123

Query: 125 RALLYIIAQCLGALCGCALVKSLQRDRYNHYGGAANQLVDGYSKGTGLAVEIVGTFVLLY 184
           RALLYIIAQCLGA+CG   VK+ Q   Y  YGG AN L DGYS GTGLA EI+GTFVL+Y
Sbjct: 124 RALLYIIAQCLGAICGVGFVKAFQSSYYTRYGGGANSLADGYSTGTGLAAEIIGTFVLVY 183

Query: 185 TVFSATDPKRNARDSYVPVLAPLPIGFTVFMVHLATIPITGTGINPARSFGAAVIINDKE 244
           TVFSATDPKR+ARDS+VPVLAPLPIGF VFMVHLATIPITGTGINPARSFGAAVI N  +
Sbjct: 184 TVFSATDPKRSARDSHVPVLAPLPIGFAVFMVHLATIPITGTGINPARSFGAAVIYNKSK 243

Query: 245 IWKDHWIFWVGPLIGATIATMYYQAVAIPSESSS 279
            W DHWIFWVGP IGA IA  Y+Q V   S S S
Sbjct: 244 PWDDHWIFWVGPFIGAAIAAFYHQFVLRASGSKS 277

BLAST of CmaCh06G003750 vs. TAIR 10
Match: AT2G37180.1 (Aquaporin-like superfamily protein )

HSP 1 Score: 398.7 bits (1023), Expect = 5.6e-111
Identity = 196/262 (74.81%), Postives = 215/262 (82.06%), Query Frame = 0

Query: 17  KDYFEPLPAPMIDMEELAKWSFYRAIIAEFVATLLFLYVVVLTVIGNKTQTDPIIGGNLC 76
           +DY +P P P  D EEL KWS YRA+IAEFVATLLFLYV VLTVIG K Q+D   GG  C
Sbjct: 14  RDYEDPPPTPFFDAEELTKWSLYRAVIAEFVATLLFLYVTVLTVIGYKIQSDTKAGGVDC 73

Query: 77  GGVGTLGIAWVFGGMIFVLVYCTAGISGGHINPAVTFGMFLGRKISLIRALLYIIAQCLG 136
           GGVG LGIAW FGGMIF+LVYCTAGISGGHINPAVTFG+FL RK+SLIRA+LY++AQCLG
Sbjct: 74  GGVGILGIAWAFGGMIFILVYCTAGISGGHINPAVTFGLFLARKVSLIRAVLYMVAQCLG 133

Query: 137 ALCGCALVKSLQRDRYNHYGGAANQLVDGYSKGTGLAVEIVGTFVLLYTVFSATDPKRNA 196
           A+CG   VK+ Q   Y +YGG AN L DGY+ GTGLA EI+GTFVL+YTVFSATDPKRNA
Sbjct: 134 AICGVGFVKAFQSSHYVNYGGGANFLADGYNTGTGLAAEIIGTFVLVYTVFSATDPKRNA 193

Query: 197 RDSYVPVLAPLPIGFTVFMVHLATIPITGTGINPARSFGAAVIINDKEIWKDHWIFWVGP 256
           RDS+VPVLAPLPIGF VFMVHLATIPITGTGINPARSFGAAVI N  + W DHWIFWVGP
Sbjct: 194 RDSHVPVLAPLPIGFAVFMVHLATIPITGTGINPARSFGAAVIFNKSKPWDDHWIFWVGP 253

Query: 257 LIGATIATMYYQAVAIPSESSS 279
            IGATIA  Y+Q V   S S S
Sbjct: 254 FIGATIAAFYHQFVLRASGSKS 275

BLAST of CmaCh06G003750 vs. TAIR 10
Match: AT2G37170.1 (plasma membrane intrinsic protein 2 )

HSP 1 Score: 396.4 bits (1017), Expect = 2.8e-110
Identity = 193/262 (73.66%), Postives = 214/262 (81.68%), Query Frame = 0

Query: 17  KDYFEPLPAPMIDMEELAKWSFYRAIIAEFVATLLFLYVVVLTVIGNKTQTDPIIGGNLC 76
           +DY +P P P  D +EL KWS YRA+IAEFVATLLFLY+ VLTVIG K Q+D   GG  C
Sbjct: 14  RDYEDPPPTPFFDADELTKWSLYRAVIAEFVATLLFLYITVLTVIGYKIQSDTKAGGVDC 73

Query: 77  GGVGTLGIAWVFGGMIFVLVYCTAGISGGHINPAVTFGMFLGRKISLIRALLYIIAQCLG 136
           GGVG LGIAW FGGMIF+LVYCTAGISGGHINPAVTFG+FL RK+SLIRA+LY++AQCLG
Sbjct: 74  GGVGILGIAWAFGGMIFILVYCTAGISGGHINPAVTFGLFLARKVSLIRAVLYMVAQCLG 133

Query: 137 ALCGCALVKSLQRDRYNHYGGAANQLVDGYSKGTGLAVEIVGTFVLLYTVFSATDPKRNA 196
           A+CG   VK+ Q   Y+ YGG AN L DGY+ GTGLA EI+GTFVL+YTVFSATDPKRNA
Sbjct: 134 AICGVGFVKAFQSSYYDRYGGGANSLADGYNTGTGLAAEIIGTFVLVYTVFSATDPKRNA 193

Query: 197 RDSYVPVLAPLPIGFTVFMVHLATIPITGTGINPARSFGAAVIINDKEIWKDHWIFWVGP 256
           RDS+VPVLAPLPIGF VFMVHLATIPITGTGINPARSFGAAVI N  + W DHWIFWVGP
Sbjct: 194 RDSHVPVLAPLPIGFAVFMVHLATIPITGTGINPARSFGAAVIYNKSKPWDDHWIFWVGP 253

Query: 257 LIGATIATMYYQAVAIPSESSS 279
            IGA IA  Y+Q V   S S S
Sbjct: 254 FIGAAIAAFYHQFVLRASGSKS 275

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9FF531.9e-11172.56Probable aquaporin PIP2-4 OS=Arabidopsis thaliana OX=3702 GN=PIP2-4 PE=1 SV=1[more]
P432862.1e-11072.99Aquaporin PIP2-1 OS=Arabidopsis thaliana OX=3702 GN=PIP2-1 PE=1 SV=1[more]
Q9ATM76.1e-11072.99Aquaporin PIP2-3 OS=Zea mays OX=4577 GN=PIP2-3 PE=2 SV=1[more]
P303027.9e-11074.81Aquaporin PIP2-3 OS=Arabidopsis thaliana OX=3702 GN=PIP2-3 PE=1 SV=1[more]
P432873.9e-10973.66Aquaporin PIP2-2 OS=Arabidopsis thaliana OX=3702 GN=PIP2-2 PE=1 SV=2[more]
Match NameE-valueIdentityDescription
A0A6J1KYL11.6e-15099.63probable aquaporin PIP2-4 OS=Cucurbita maxima OX=3661 GN=LOC111498744 PE=3 SV=1[more]
A0A6J1H8471.5e-14897.78probable aquaporin PIP2-4 OS=Cucurbita moschata OX=3662 GN=LOC111461325 PE=3 SV=... [more]
A0A5A7TKK04.3e-13587.41Putative aquaporin PIP2-4 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffo... [more]
A0A1S3B6994.3e-13587.41probable aquaporin PIP2-4 OS=Cucumis melo OX=3656 GN=LOC103486477 PE=3 SV=1[more]
A0A0A0LDZ72.0e-13285.56Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G739030 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
KAG6596471.11.4e-20192.35putative aquaporin PIP2-4, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_023005870.13.4e-15099.63probable aquaporin PIP2-4 [Cucurbita maxima][more]
XP_023540178.12.4e-14898.15probable aquaporin PIP2-4 [Cucurbita pepo subsp. pepo][more]
XP_022960621.13.2e-14897.78probable aquaporin PIP2-4 [Cucurbita moschata][more]
NP_001380714.19.0e-13587.41aquaporin PIP2-8 [Cucumis melo] >KAA0044013.1 putative aquaporin PIP2-4 [Cucumis... [more]
Match NameE-valueIdentityDescription
AT5G60660.11.3e-11272.56plasma membrane intrinsic protein 2;4 [more]
AT3G53420.11.5e-11172.99plasma membrane intrinsic protein 2A [more]
AT3G53420.21.5e-11172.99plasma membrane intrinsic protein 2A [more]
AT2G37180.15.6e-11174.81Aquaporin-like superfamily protein [more]
AT2G37170.12.8e-11073.66plasma membrane intrinsic protein 2 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 314..348
NoneNo IPR availableGENE3D1.20.5.2950coord: 289..399
e-value: 1.2E-33
score: 117.7
NoneNo IPR availablePANTHERPTHR45687:SF39AQUAPORIN PIP2-4-RELATEDcoord: 6..271
IPR000425Major intrinsic proteinPRINTSPR00783MINTRINSICPcoord: 174..192
score: 50.49
coord: 210..232
score: 57.01
coord: 125..144
score: 50.15
coord: 250..270
score: 46.16
coord: 88..112
score: 62.61
coord: 40..59
score: 42.52
IPR000425Major intrinsic proteinPFAMPF00230MIPcoord: 33..267
e-value: 1.8E-80
score: 269.9
IPR000425Major intrinsic proteinTIGRFAMTIGR00861TIGR00861coord: 44..267
e-value: 1.0E-61
score: 206.5
IPR000425Major intrinsic proteinCDDcd00333MIPcoord: 40..268
e-value: 3.05823E-82
score: 249.478
IPR005124Vacuolar (H+)-ATPase G subunitTIGRFAMTIGR01147TIGR01147coord: 291..398
e-value: 1.1E-23
score: 81.8
IPR005124Vacuolar (H+)-ATPase G subunitPFAMPF03179V-ATPase_Gcoord: 296..398
e-value: 5.3E-32
score: 110.5
IPR023271Aquaporin-likeGENE3D1.20.1080.10Glycerol uptake facilitator protein.coord: 25..279
e-value: 8.5E-87
score: 292.4
IPR023271Aquaporin-likeSUPERFAMILY81338Aquaporin-likecoord: 31..276
IPR034294Aquaporin transporterPANTHERPTHR45687AQUAPORIN OR AQUAGLYCEROPORIN RELATEDcoord: 6..271
IPR022357Major intrinsic protein, conserved sitePROSITEPS00221MIPcoord: 106..114

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh06G003750.1CmaCh06G003750.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:1902600 proton transmembrane transport
biological_process GO:0006833 water transport
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005886 plasma membrane
cellular_component GO:0016471 vacuolar proton-transporting V-type ATPase complex
cellular_component GO:0016020 membrane
molecular_function GO:0042626 ATPase-coupled transmembrane transporter activity
molecular_function GO:0015250 water channel activity
molecular_function GO:0015267 channel activity