CmaCh06G002030 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh06G002030
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
DescriptionNAD(P)-binding Rossmann-fold superfamily protein
LocationCma_Chr06: 1049388 .. 1050395 (-)
RNA-Seq ExpressionCmaCh06G002030
SyntenyCmaCh06G002030
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGACTGCCCAAGTGCTGCCCGAGCAAGCGCTTCAAAGCAATATTCATCTCTTGCAAAGGGACAGCCCTACCCCTTCCTATAAAAGGTATTAAAATAATATATATGTATGTGTGTGTGTGTGTATGTATCTATGTATGTATCTATGTATGTATGTATGTATCTTAGCTTGATGTTTTGGTTTTAGGTTGGAGGGAAAGGTGGCGATTGTAACGGGCGGCGCAAAGGGAATCGGAGAAGCGACGGTGAGGCTGTTCGCCAAACACGGAGCCAAAGTAGTGATCGCCGACGTGGAGGATCTCCTCGGTGAGGCTTTGGCCGACACGCTTTCTCCAAATCCAGTGACGTTCGTCCACTGTGACGTAAGCTTGGAGGAAGACATGGAGAATCTAATCAACTCGACGATGTGTCGTCACGGCCAAGTCGACATACTATTCAACAATGCCGGAGTTTTAGGTAACCAATCCAAGACTCGTAAGAGCATCGTTGATTTTGACCCTGACGAGTTCGAGCGCGTGATGTGTGTGAACGTCAAAGGCGTCGCGTTGGGCATAAAGCACGCGGCGCGTGTGATGATTCCCAGAGCTACCGGCTGTATAATCTCGACTGCTAGCGTGGCAGGTGTTTCAGGAGGTATGGGTCCACATGCCTACACAGCATCCAAACATGCCATTGTTGGCCTCACTAAGAACACTGCCTGCGAGCTTGGGCGTTACGGTATACGTGTCAACTGCATTTCGCCGTTTGGAGTTGCCACGTCGATGCTTGTGAATGCGTGGAGGGATGACGGAGACGAGTGCATGAACTTCGGGATTCCATCGCCAGCTGAGGTGGACAAAATGGAGGAGTTCGTTAGGGGTTTGGCCAATCTTAAGGGCCCCACATTGAGGCCTAAGGATATTGCTGAGGCGGCGCTTTATCTTGCCAGCGATGAATCTAAGTACGTCAGTGGCCATAATCTCGTTGTTGATGGTGGAATCACCACTTCAAGAAATTGTATCGGCTTGTAA

mRNA sequence

ATGACTGCCCAAGTGCTGCCCGAGCAAGCGCTTCAAAGCAATATTCATCTCTTGCAAAGGGACAGCCCTACCCCTTCCTATAAAAGGTTGGAGGGAAAGGTGGCGATTGTAACGGGCGGCGCAAAGGGAATCGGAGAAGCGACGGTGAGGCTGTTCGCCAAACACGGAGCCAAAGTAGTGATCGCCGACGTGGAGGATCTCCTCGGTGAGGCTTTGGCCGACACGCTTTCTCCAAATCCAGTGACGTTCGTCCACTGTGACGTAAGCTTGGAGGAAGACATGGAGAATCTAATCAACTCGACGATGTGTCGTCACGGCCAAGTCGACATACTATTCAACAATGCCGGAGTTTTAGGTAACCAATCCAAGACTCGTAAGAGCATCGTTGATTTTGACCCTGACGAGTTCGAGCGCGTGATGTGTGTGAACGTCAAAGGCGTCGCGTTGGGCATAAAGCACGCGGCGCGTGTGATGATTCCCAGAGCTACCGGCTGTATAATCTCGACTGCTAGCGTGGCAGGTGTTTCAGGAGGTATGGGTCCACATGCCTACACAGCATCCAAACATGCCATTGTTGGCCTCACTAAGAACACTGCCTGCGAGCTTGGGCGTTACGGTATACGTGTCAACTGCATTTCGCCGTTTGGAGTTGCCACGTCGATGCTTGTGAATGCGTGGAGGGATGACGGAGACGAGTGCATGAACTTCGGGATTCCATCGCCAGCTGAGGTGGACAAAATGGAGGAGTTCGTTAGGGGTTTGGCCAATCTTAAGGGCCCCACATTGAGGCCTAAGGATATTGCTGAGGCGGCGCTTTATCTTGCCAGCGATGAATCTAAGTACGTCAGTGGCCATAATCTCGTTGTTGATGGTGGAATCACCACTTCAAGAAATTGTATCGGCTTGTAA

Coding sequence (CDS)

ATGACTGCCCAAGTGCTGCCCGAGCAAGCGCTTCAAAGCAATATTCATCTCTTGCAAAGGGACAGCCCTACCCCTTCCTATAAAAGGTTGGAGGGAAAGGTGGCGATTGTAACGGGCGGCGCAAAGGGAATCGGAGAAGCGACGGTGAGGCTGTTCGCCAAACACGGAGCCAAAGTAGTGATCGCCGACGTGGAGGATCTCCTCGGTGAGGCTTTGGCCGACACGCTTTCTCCAAATCCAGTGACGTTCGTCCACTGTGACGTAAGCTTGGAGGAAGACATGGAGAATCTAATCAACTCGACGATGTGTCGTCACGGCCAAGTCGACATACTATTCAACAATGCCGGAGTTTTAGGTAACCAATCCAAGACTCGTAAGAGCATCGTTGATTTTGACCCTGACGAGTTCGAGCGCGTGATGTGTGTGAACGTCAAAGGCGTCGCGTTGGGCATAAAGCACGCGGCGCGTGTGATGATTCCCAGAGCTACCGGCTGTATAATCTCGACTGCTAGCGTGGCAGGTGTTTCAGGAGGTATGGGTCCACATGCCTACACAGCATCCAAACATGCCATTGTTGGCCTCACTAAGAACACTGCCTGCGAGCTTGGGCGTTACGGTATACGTGTCAACTGCATTTCGCCGTTTGGAGTTGCCACGTCGATGCTTGTGAATGCGTGGAGGGATGACGGAGACGAGTGCATGAACTTCGGGATTCCATCGCCAGCTGAGGTGGACAAAATGGAGGAGTTCGTTAGGGGTTTGGCCAATCTTAAGGGCCCCACATTGAGGCCTAAGGATATTGCTGAGGCGGCGCTTTATCTTGCCAGCGATGAATCTAAGTACGTCAGTGGCCATAATCTCGTTGTTGATGGTGGAATCACCACTTCAAGAAATTGTATCGGCTTGTAA

Protein sequence

MTAQVLPEQALQSNIHLLQRDSPTPSYKRLEGKVAIVTGGAKGIGEATVRLFAKHGAKVVIADVEDLLGEALADTLSPNPVTFVHCDVSLEEDMENLINSTMCRHGQVDILFNNAGVLGNQSKTRKSIVDFDPDEFERVMCVNVKGVALGIKHAARVMIPRATGCIISTASVAGVSGGMGPHAYTASKHAIVGLTKNTACELGRYGIRVNCISPFGVATSMLVNAWRDDGDECMNFGIPSPAEVDKMEEFVRGLANLKGPTLRPKDIAEAALYLASDESKYVSGHNLVVDGGITTSRNCIGL
Homology
BLAST of CmaCh06G002030 vs. ExPASy Swiss-Prot
Match: Q9SCU0 (Short-chain dehydrogenase reductase 2a OS=Arabidopsis thaliana OX=3702 GN=SDR2a PE=3 SV=1)

HSP 1 Score: 395.2 bits (1014), Expect = 6.6e-109
Identity = 208/309 (67.31%), Postives = 245/309 (79.29%), Query Frame = 0

Query: 1   MTAQVLPEQALQSNIH--LLQRDSPTPSYKRLEGKVAIVTGGAKGIGEATVRLFAKHGAK 60
           M AQV+ EQ    ++H  +++  + T   KRLEGKVAI+TGGA GIG+ATV LFA+HGA 
Sbjct: 1   MPAQVIAEQTTFHSVHDTIMEETNTTLYPKRLEGKVAIITGGAHGIGKATVMLFARHGAT 60

Query: 61  VVIADVEDLLGEALADTLSPNP----VTFVHCDVSLEEDMENLINSTMCRHGQVDILFNN 120
           VVIADV+++ G +LA +LS +     V F+ CDVS+E D+ENL+N T+ R+G++DILFNN
Sbjct: 61  VVIADVDNVAGSSLAKSLSSHKTSPMVAFISCDVSVEADVENLVNVTVARYGRLDILFNN 120

Query: 121 AGVLGNQSKTRKSIVDFDPDEFERVMCVNVKGVALGIKHAARVMIPRA-TGCIISTASVA 180
           AGVLG+Q K  KSI+DFD DEF+ VM VNV+GV LG+KH AR MI R   GCIISTASVA
Sbjct: 121 AGVLGDQKK-HKSILDFDADEFDHVMRVNVRGVGLGMKHGARAMIKRGFKGCIISTASVA 180

Query: 181 GVSGGMGPHAYTASKHAIVGLTKNTACELGRYGIRVNCISPFGVATSMLVNAWRDDGDEC 240
           GV GGMGPHAYTASKHAIVGLTKN ACELG+YGIRVNCISPFGVATSMLVNAWR      
Sbjct: 181 GVMGGMGPHAYTASKHAIVGLTKNAACELGKYGIRVNCISPFGVATSMLVNAWRKTSG-- 240

Query: 241 MNFGIPSPAEVDKMEEFVRGLANLKGPTLRPKDIAEAALYLASDESKYVSGHNLVVDGGI 300
              G     +V++MEEFVR LANLKG TLR  DIAEAALYLASDESKYV+GHNLVVDGG+
Sbjct: 241 ---GDVEDDDVEEMEEFVRSLANLKGETLRANDIAEAALYLASDESKYVNGHNLVVDGGV 300

Query: 301 TTSRNCIGL 303
           TT+RNC+GL
Sbjct: 301 TTARNCVGL 303

BLAST of CmaCh06G002030 vs. ExPASy Swiss-Prot
Match: P50160 (Sex determination protein tasselseed-2 OS=Zea mays OX=4577 GN=TS2 PE=2 SV=1)

HSP 1 Score: 375.9 bits (964), Expect = 4.2e-103
Identity = 188/292 (64.38%), Postives = 227/292 (77.74%), Query Frame = 0

Query: 23  PTPSYKRLEGKVAIVTGGAKGIGEATVRLFAKHGAKVVIADVEDLLGEALADTLSPNPVT 82
           PTP  KRL+GKVAIVTGGA+GIGEA VRLFAKHGA+VVIAD++D  GEALA  L P  V+
Sbjct: 46  PTPMPKRLDGKVAIVTGGARGIGEAIVRLFAKHGARVVIADIDDAAGEALASALGPQ-VS 105

Query: 83  FVHCDVSLEEDMENLINSTMCRH-GQVDILFNNAGVLGNQSKTRKSIVDFDPDEFERVMC 142
           FV CDVS+E+D+   ++  + RH G++D+  NNAGVLG Q++  +SI+ FD  EF+RV+ 
Sbjct: 106 FVRCDVSVEDDVRRAVDWALSRHGGRLDVYCNNAGVLGRQTRAARSILSFDAAEFDRVLR 165

Query: 143 VNVKGVALGIKHAARVMIPRATGCIISTASVAGVSGGMGPHAYTASKHAIVGLTKNTACE 202
           VN  G ALG+KHAAR M PR  G I+S ASVA V GG+GPHAYTASKHAIVGLTKN ACE
Sbjct: 166 VNALGAALGMKHAARAMAPRRAGSIVSVASVAAVLGGLGPHAYTASKHAIVGLTKNAACE 225

Query: 203 LGRYGIRVNCISPFGVATSMLVNAWRDDGDEC-----------MNFGIPSPAEVDKMEEF 262
           L  +G+RVNC+SPFGVAT ML+NAWR   D+            ++  +PS  EV+KMEE 
Sbjct: 226 LRAHGVRVNCVSPFGVATPMLINAWRQGHDDATADADRDLDLDLDVTVPSDQEVEKMEEV 285

Query: 263 VRGLANLKGPTLRPKDIAEAALYLASDESKYVSGHNLVVDGGITTSRNCIGL 303
           VRGLA LKGPTLRP+DIAEA L+LASDE++Y+SGHNLVVDGG+TTSRN IGL
Sbjct: 286 VRGLATLKGPTLRPRDIAEAVLFLASDEARYISGHNLVVDGGVTTSRNLIGL 336

BLAST of CmaCh06G002030 vs. ExPASy Swiss-Prot
Match: Q94KL7 (Secoisolariciresinol dehydrogenase (Fragment) OS=Forsythia intermedia OX=55183 PE=1 SV=1)

HSP 1 Score: 264.6 bits (675), Expect = 1.4e-69
Identity = 135/273 (49.45%), Postives = 189/273 (69.23%), Query Frame = 0

Query: 28  KRLEGKVAIVTGGAKGIGEATVRLFAKHGAKVVIADVEDLLGEALADTLSPNPVTFVHCD 87
           +RLEGKVA++TGGA GIGE T +LF++HGAKV IADV+D LG ++ + +  +  T++HCD
Sbjct: 13  RRLEGKVALITGGASGIGETTAKLFSQHGAKVAIADVQDELGHSVVEAIGTSNSTYIHCD 72

Query: 88  VSLEEDMENLINSTMCRHGQVDILFNNAGVLGNQSKTRKSIVDFDPDEFERVMCVNVKGV 147
           V+ E+ ++N +++T+  +G++DI+F+NAG+       R  I+D +  +FERV  VNV GV
Sbjct: 73  VTNEDGVKNAVDNTVSTYGKLDIMFSNAGI---SDPNRPRIIDNEKADFERVFSVNVTGV 132

Query: 148 ALGIKHAARVMIPRATGCIISTASVAGVSGGMGPHAYTASKHAIVGLTKNTACELGRYGI 207
            L +KHAARVMIP  +G IISTAS++   GG   HAY  SKHA++GLT+N A ELG++GI
Sbjct: 133 FLCMKHAARVMIPARSGNIISTASLSSTMGGGSSHAYCGSKHAVLGLTRNLAVELGQFGI 192

Query: 208 RVNCISPFGVATSMLVNAWRDDGDECMNFGIPSPAEVDKMEEFVRGLANLKGPTLRPKDI 267
           RVNC+SPFG+ T++        G +    GI +  E + +  F     NLKGP    +D+
Sbjct: 193 RVNCLSPFGLPTAL--------GKKFS--GIKNEEEFENVINFA---GNLKGPKFNVEDV 252

Query: 268 AEAALYLASDESKYVSGHNLVVDGGITTSRNCI 301
           A AALYLASDE+KYVSGHNL +DGG +   + I
Sbjct: 253 ANAALYLASDEAKYVSGHNLFIDGGFSVCNSVI 269

BLAST of CmaCh06G002030 vs. ExPASy Swiss-Prot
Match: F1SWA0 (Zerumbone synthase OS=Zingiber zerumbet OX=311405 GN=ZSD1 PE=1 SV=1)

HSP 1 Score: 260.0 bits (663), Expect = 3.3e-68
Identity = 136/276 (49.28%), Postives = 186/276 (67.39%), Query Frame = 0

Query: 29  RLEGKVAIVTGGAKGIGEATVRLFAKHGAKVVIADVEDLLGEALADTLSPNP-VTFVHCD 88
           RLEGKVA+VTGGA GIGE+  RLF +HGAK+ I DV+D LG+ ++  L  +P   + HCD
Sbjct: 2   RLEGKVALVTGGASGIGESIARLFIEHGAKICIVDVQDELGQQVSQRLGGDPHACYFHCD 61

Query: 89  VSLEEDMENLINSTMCRHGQVDILFNNAGVLGNQSKTRKSIVDFDPDEFERVMCVNVKGV 148
           V++E+D+   ++ T  ++G +DI+ NNAG+ G++      I D D +EF++V  +NV GV
Sbjct: 62  VTVEDDVRRAVDFTAEKYGTIDIMVNNAGITGDKV---IDIRDADFNEFKKVFDINVNGV 121

Query: 149 ALGIKHAARVMIPRATGCIISTASVAGVSGGMGPHAYTASKHAIVGLTKNTACELGRYGI 208
            LG+KHAAR+MIP+  G I+S ASV+ V  G GPH YT +KHA+VGLTK+ A ELGR+GI
Sbjct: 122 FLGMKHAARIMIPKMKGSIVSLASVSSVIAGAGPHGYTGAKHAVVGLTKSVAAELGRHGI 181

Query: 209 RVNCISPFGVATSMLVNAWRDDGDECMNFGIPSPAEVDKME---EFVRGLANLKGPTLRP 268
           RVNC+SP+ V T +            M +   S  + D +     FVR  ANLKG  L P
Sbjct: 182 RVNCVSPYAVPTRL-----------SMPYLPESEMQEDALRGFLTFVRSNANLKGVDLMP 241

Query: 269 KDIAEAALYLASDESKYVSGHNLVVDGGITTSRNCI 301
            D+AEA LYLA++ESKYVSG NLV+DGG + + + +
Sbjct: 242 NDVAEAVLYLATEESKYVSGLNLVIDGGFSIANHTL 263

BLAST of CmaCh06G002030 vs. ExPASy Swiss-Prot
Match: Q94KL8 (Secoisolariciresinol dehydrogenase (Fragment) OS=Podophyllum peltatum OX=35933 PE=1 SV=1)

HSP 1 Score: 258.5 bits (659), Expect = 9.7e-68
Identity = 137/275 (49.82%), Postives = 188/275 (68.36%), Query Frame = 0

Query: 22  SPTPSYKRLEGKVAIVTGGAKGIGEATVRLFAKHGAKVVIADVEDLLGEALADTL-SPNP 81
           +P  S  RL+ KVAI+TGGA GIGE T +LF ++GAKVVIAD+ D  G+ + + + SP+ 
Sbjct: 6   TPDSSTNRLQDKVAIITGGAGGIGETTAKLFVRYGAKVVIADIADDHGQKVCNNIGSPDV 65

Query: 82  VTFVHCDVSLEEDMENLINSTMCRHGQVDILFNNAGVLGNQSKTRKSIVDFDPDEFERVM 141
           ++FVHCDV+ +ED+ NL+++T+ +HG++DI+F N GVL   S T  SI++   ++F+RVM
Sbjct: 66  ISFVHCDVTKDEDVRNLVDTTIAKHGKLDIMFGNVGVL---STTPYSILEAGNEDFKRVM 125

Query: 142 CVNVKGVALGIKHAARVMIPRATGCIISTASVAGVSGGMG-PHAYTASKHAIVGLTKNTA 201
            +NV G  L  KHAARVMIP   G I+ TAS++  + G G  H YTA+KHA++GLT +  
Sbjct: 126 DINVYGAFLVAKHAARVMIPAKKGSIVFTASISSFTAGEGVSHVYTATKHAVLGLTTSLC 185

Query: 202 CELGRYGIRVNCISPFGVATSMLVNAWRDDGDECMNFGIPSPAEVDKMEEFVRGLANLKG 261
            ELG YGIRVNC+SP+ VA+ +L +           FG+ S     ++EE     ANLKG
Sbjct: 186 TELGEYGIRVNCVSPYIVASPLLTDV----------FGVDS----SRVEELAHQAANLKG 245

Query: 262 PTLRPKDIAEAALYLASDESKYVSGHNLVVDGGIT 295
             LR +D+A+A  YLA DESKYVSG NLV+DGG T
Sbjct: 246 TLLRAEDVADAVAYLAGDESKYVSGLNLVIDGGYT 263

BLAST of CmaCh06G002030 vs. ExPASy TrEMBL
Match: A0A6J1I659 (short-chain dehydrogenase reductase 2a-like OS=Cucurbita maxima OX=3661 GN=LOC111470263 PE=4 SV=1)

HSP 1 Score: 606.7 bits (1563), Expect = 5.4e-170
Identity = 302/302 (100.00%), Postives = 302/302 (100.00%), Query Frame = 0

Query: 1   MTAQVLPEQALQSNIHLLQRDSPTPSYKRLEGKVAIVTGGAKGIGEATVRLFAKHGAKVV 60
           MTAQVLPEQALQSNIHLLQRDSPTPSYKRLEGKVAIVTGGAKGIGEATVRLFAKHGAKVV
Sbjct: 1   MTAQVLPEQALQSNIHLLQRDSPTPSYKRLEGKVAIVTGGAKGIGEATVRLFAKHGAKVV 60

Query: 61  IADVEDLLGEALADTLSPNPVTFVHCDVSLEEDMENLINSTMCRHGQVDILFNNAGVLGN 120
           IADVEDLLGEALADTLSPNPVTFVHCDVSLEEDMENLINSTMCRHGQVDILFNNAGVLGN
Sbjct: 61  IADVEDLLGEALADTLSPNPVTFVHCDVSLEEDMENLINSTMCRHGQVDILFNNAGVLGN 120

Query: 121 QSKTRKSIVDFDPDEFERVMCVNVKGVALGIKHAARVMIPRATGCIISTASVAGVSGGMG 180
           QSKTRKSIVDFDPDEFERVMCVNVKGVALGIKHAARVMIPRATGCIISTASVAGVSGGMG
Sbjct: 121 QSKTRKSIVDFDPDEFERVMCVNVKGVALGIKHAARVMIPRATGCIISTASVAGVSGGMG 180

Query: 181 PHAYTASKHAIVGLTKNTACELGRYGIRVNCISPFGVATSMLVNAWRDDGDECMNFGIPS 240
           PHAYTASKHAIVGLTKNTACELGRYGIRVNCISPFGVATSMLVNAWRDDGDECMNFGIPS
Sbjct: 181 PHAYTASKHAIVGLTKNTACELGRYGIRVNCISPFGVATSMLVNAWRDDGDECMNFGIPS 240

Query: 241 PAEVDKMEEFVRGLANLKGPTLRPKDIAEAALYLASDESKYVSGHNLVVDGGITTSRNCI 300
           PAEVDKMEEFVRGLANLKGPTLRPKDIAEAALYLASDESKYVSGHNLVVDGGITTSRNCI
Sbjct: 241 PAEVDKMEEFVRGLANLKGPTLRPKDIAEAALYLASDESKYVSGHNLVVDGGITTSRNCI 300

Query: 301 GL 303
           GL
Sbjct: 301 GL 302

BLAST of CmaCh06G002030 vs. ExPASy TrEMBL
Match: A0A6J1G6Z3 (short-chain dehydrogenase reductase 2a OS=Cucurbita moschata OX=3662 GN=LOC111451343 PE=4 SV=1)

HSP 1 Score: 602.1 bits (1551), Expect = 1.3e-168
Identity = 299/302 (99.01%), Postives = 301/302 (99.67%), Query Frame = 0

Query: 1   MTAQVLPEQALQSNIHLLQRDSPTPSYKRLEGKVAIVTGGAKGIGEATVRLFAKHGAKVV 60
           MTAQVLPEQALQSNIHLLQRD+P PSYKRLEGKVAIVTGGAKGIGEATVRLFAKHGAKVV
Sbjct: 1   MTAQVLPEQALQSNIHLLQRDTPAPSYKRLEGKVAIVTGGAKGIGEATVRLFAKHGAKVV 60

Query: 61  IADVEDLLGEALADTLSPNPVTFVHCDVSLEEDMENLINSTMCRHGQVDILFNNAGVLGN 120
           IADVEDLLGEALADTLSPNPV+FVHCDVSLEEDMENLINSTMCRHGQVDILFNNAGVLGN
Sbjct: 61  IADVEDLLGEALADTLSPNPVSFVHCDVSLEEDMENLINSTMCRHGQVDILFNNAGVLGN 120

Query: 121 QSKTRKSIVDFDPDEFERVMCVNVKGVALGIKHAARVMIPRATGCIISTASVAGVSGGMG 180
           QSKTRKSIVDFDPDEFERVMCVNVKGVALGIKHAARVMIPRATGCIISTASVAGVSGGMG
Sbjct: 121 QSKTRKSIVDFDPDEFERVMCVNVKGVALGIKHAARVMIPRATGCIISTASVAGVSGGMG 180

Query: 181 PHAYTASKHAIVGLTKNTACELGRYGIRVNCISPFGVATSMLVNAWRDDGDECMNFGIPS 240
           PHAYTASKHAIVGLTKNTACELGRYGIRVNCISPFGVATSMLVNAWRDDGDECMNFGIPS
Sbjct: 181 PHAYTASKHAIVGLTKNTACELGRYGIRVNCISPFGVATSMLVNAWRDDGDECMNFGIPS 240

Query: 241 PAEVDKMEEFVRGLANLKGPTLRPKDIAEAALYLASDESKYVSGHNLVVDGGITTSRNCI 300
           PAEVDKMEEFVRGLANLKGPTLRPKDIAEAALYLASDESKYVSGHNLVVDGGITTSRNCI
Sbjct: 241 PAEVDKMEEFVRGLANLKGPTLRPKDIAEAALYLASDESKYVSGHNLVVDGGITTSRNCI 300

Query: 301 GL 303
           GL
Sbjct: 301 GL 302

BLAST of CmaCh06G002030 vs. ExPASy TrEMBL
Match: A0A6J1F7B6 (short-chain dehydrogenase reductase 2a-like OS=Cucurbita moschata OX=3662 GN=LOC111441518 PE=4 SV=1)

HSP 1 Score: 549.7 bits (1415), Expect = 7.8e-153
Identity = 277/305 (90.82%), Postives = 290/305 (95.08%), Query Frame = 0

Query: 1   MTAQVLPEQALQSNIHLLQRDSPTPSYKRLEGKVAIVTGGAKGIGEATVRLFAKHGAKVV 60
           MTAQVLPEQALQSNIHLL RD+    +KRLEGKVAIVTGGAKGIGEATVRLFAKHGAKVV
Sbjct: 1   MTAQVLPEQALQSNIHLLGRDNSPSLHKRLEGKVAIVTGGAKGIGEATVRLFAKHGAKVV 60

Query: 61  IADVEDLLGEALADTLSPNPVTFVHCDVSLEEDMENLINSTMCRHGQVDILFNNAGVLGN 120
           IADVEDLLG+ALADTLSP+PV+FVHCDVS EED+ENLI+ST+CRHGQVDI+FNNAGVLGN
Sbjct: 61  IADVEDLLGQALADTLSPSPVSFVHCDVSSEEDVENLISSTVCRHGQVDIIFNNAGVLGN 120

Query: 121 QSKTRKSIVDFDPDEFERVMCVNVKGVALGIKHAARVMIPRATGCIISTASVAGVSGGMG 180
           QSKT KSIVDFDP+EFERVM VNVKGVALGIKHAARVMIPRATGCIISTASVAGV GG+G
Sbjct: 121 QSKTHKSIVDFDPNEFERVMRVNVKGVALGIKHAARVMIPRATGCIISTASVAGVLGGLG 180

Query: 181 PHAYTASKHAIVGLTKNTACELGRYGIRVNCISPFGVATSMLVNAWRDDG---DECMNFG 240
           PHAYTASKHAIVGLTKNTACELGRYGIRVNCISPFGVATSMLVNAWR DG   DECMN G
Sbjct: 181 PHAYTASKHAIVGLTKNTACELGRYGIRVNCISPFGVATSMLVNAWRADGDEEDECMNLG 240

Query: 241 IPSPAEVDKMEEFVRGLANLKGPTLRPKDIAEAALYLASDESKYVSGHNLVVDGGITTSR 300
           IPS AEVDKMEEFVRGL+NLKGPTLRPKDIAEAALYLASDESKY+SGHNLVVDGGITTSR
Sbjct: 241 IPSVAEVDKMEEFVRGLSNLKGPTLRPKDIAEAALYLASDESKYISGHNLVVDGGITTSR 300

Query: 301 NCIGL 303
           NCIGL
Sbjct: 301 NCIGL 305

BLAST of CmaCh06G002030 vs. ExPASy TrEMBL
Match: A0A6J1J5H2 (short-chain dehydrogenase reductase 2a-like OS=Cucurbita maxima OX=3661 GN=LOC111481921 PE=4 SV=1)

HSP 1 Score: 544.3 bits (1401), Expect = 3.3e-151
Identity = 275/305 (90.16%), Postives = 289/305 (94.75%), Query Frame = 0

Query: 1   MTAQVLPEQALQSNIHLLQRDSPTPSYKRLEGKVAIVTGGAKGIGEATVRLFAKHGAKVV 60
           MTAQVLPEQALQ+NI LL RD+    +KRLEGKVAIVTGGAKGIGEATVRLFAKHGAKVV
Sbjct: 1   MTAQVLPEQALQTNILLLGRDNSPSLHKRLEGKVAIVTGGAKGIGEATVRLFAKHGAKVV 60

Query: 61  IADVEDLLGEALADTLSPNPVTFVHCDVSLEEDMENLINSTMCRHGQVDILFNNAGVLGN 120
           IADVEDLLG+ALADTLSP+PV+FVHCDVS EED+ENLI+ST+CRHGQVDI+FNNAGVLGN
Sbjct: 61  IADVEDLLGQALADTLSPSPVSFVHCDVSSEEDVENLISSTVCRHGQVDIIFNNAGVLGN 120

Query: 121 QSKTRKSIVDFDPDEFERVMCVNVKGVALGIKHAARVMIPRATGCIISTASVAGVSGGMG 180
           QSKT KSIVDFDP+EFERVM VNVKGVALGIKHAARVMIPRATGCIISTASVAGV GG+G
Sbjct: 121 QSKTHKSIVDFDPNEFERVMRVNVKGVALGIKHAARVMIPRATGCIISTASVAGVLGGLG 180

Query: 181 PHAYTASKHAIVGLTKNTACELGRYGIRVNCISPFGVATSMLVNAWRDDG---DECMNFG 240
           PHAYTASKHAIVGLTKNTACELGRYGIRVNCISPFGVATSMLVNAWR DG   DECMN G
Sbjct: 181 PHAYTASKHAIVGLTKNTACELGRYGIRVNCISPFGVATSMLVNAWRADGDEEDECMNLG 240

Query: 241 IPSPAEVDKMEEFVRGLANLKGPTLRPKDIAEAALYLASDESKYVSGHNLVVDGGITTSR 300
           IPS AEVDKMEEFVRGL+NLKGPTLRPKDIAEAALYLASDESKY+SGHNLVVDGGITTSR
Sbjct: 241 IPSVAEVDKMEEFVRGLSNLKGPTLRPKDIAEAALYLASDESKYISGHNLVVDGGITTSR 300

Query: 301 NCIGL 303
           NCIGL
Sbjct: 301 NCIGL 305

BLAST of CmaCh06G002030 vs. ExPASy TrEMBL
Match: A0A0A0LF57 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G730750 PE=4 SV=1)

HSP 1 Score: 534.6 bits (1376), Expect = 2.6e-148
Identity = 265/308 (86.04%), Postives = 291/308 (94.48%), Query Frame = 0

Query: 1   MTAQVLPEQALQSNIHLLQRD---SPTPSYKRLEGKVAIVTGGAKGIGEATVRLFAKHGA 60
           MTAQVLPEQ+LQSNIHLL RD   SP P +KRLEGKVAIVTGGAKGIGEATVRLFAKHGA
Sbjct: 1   MTAQVLPEQSLQSNIHLLVRDNSSSPPPFHKRLEGKVAIVTGGAKGIGEATVRLFAKHGA 60

Query: 61  KVVIADVEDLLGEALADTLSPNPVTFVHCDVSLEEDMENLINSTMCRHGQVDILFNNAGV 120
           KVVIADVED+LG+ALA+TLSP+PV+FVHCDVS E+D+ENL+++T+C HGQVDI+FNNAGV
Sbjct: 61  KVVIADVEDILGQALANTLSPSPVSFVHCDVSSEDDVENLVSTTVCLHGQVDIIFNNAGV 120

Query: 121 LGNQSKTRKSIVDFDPDEFERVMCVNVKGVALGIKHAARVMIPRATGCIISTASVAGVSG 180
           LGNQS + KSI+DFDPDEFERVM VNVKGVALGIKHAARVMIPRATGCIISTASVAGV G
Sbjct: 121 LGNQSNSHKSILDFDPDEFERVMRVNVKGVALGIKHAARVMIPRATGCIISTASVAGVLG 180

Query: 181 GMGPHAYTASKHAIVGLTKNTACELGRYGIRVNCISPFGVATSMLVNAWR---DDGDECM 240
           G+GPHAYTASKHAIVGLTKNTACELGRYGIRVNCISPFGVATSMLVNAWR   ++ +ECM
Sbjct: 181 GLGPHAYTASKHAIVGLTKNTACELGRYGIRVNCISPFGVATSMLVNAWRADVEEEEECM 240

Query: 241 NFGIPSPAEVDKMEEFVRGLANLKGPTLRPKDIAEAALYLASDESKYVSGHNLVVDGGIT 300
           N+G+PS AEVDKMEEFVRGLANLKGPTL+PKDIA+AALYLASDESKY+SGHNLVVDGG+T
Sbjct: 241 NYGVPSAAEVDKMEEFVRGLANLKGPTLKPKDIAQAALYLASDESKYISGHNLVVDGGVT 300

Query: 301 TSRNCIGL 303
           TSRNCIGL
Sbjct: 301 TSRNCIGL 308

BLAST of CmaCh06G002030 vs. NCBI nr
Match: XP_022971590.1 (short-chain dehydrogenase reductase 2a-like [Cucurbita maxima])

HSP 1 Score: 606.7 bits (1563), Expect = 1.1e-169
Identity = 302/302 (100.00%), Postives = 302/302 (100.00%), Query Frame = 0

Query: 1   MTAQVLPEQALQSNIHLLQRDSPTPSYKRLEGKVAIVTGGAKGIGEATVRLFAKHGAKVV 60
           MTAQVLPEQALQSNIHLLQRDSPTPSYKRLEGKVAIVTGGAKGIGEATVRLFAKHGAKVV
Sbjct: 1   MTAQVLPEQALQSNIHLLQRDSPTPSYKRLEGKVAIVTGGAKGIGEATVRLFAKHGAKVV 60

Query: 61  IADVEDLLGEALADTLSPNPVTFVHCDVSLEEDMENLINSTMCRHGQVDILFNNAGVLGN 120
           IADVEDLLGEALADTLSPNPVTFVHCDVSLEEDMENLINSTMCRHGQVDILFNNAGVLGN
Sbjct: 61  IADVEDLLGEALADTLSPNPVTFVHCDVSLEEDMENLINSTMCRHGQVDILFNNAGVLGN 120

Query: 121 QSKTRKSIVDFDPDEFERVMCVNVKGVALGIKHAARVMIPRATGCIISTASVAGVSGGMG 180
           QSKTRKSIVDFDPDEFERVMCVNVKGVALGIKHAARVMIPRATGCIISTASVAGVSGGMG
Sbjct: 121 QSKTRKSIVDFDPDEFERVMCVNVKGVALGIKHAARVMIPRATGCIISTASVAGVSGGMG 180

Query: 181 PHAYTASKHAIVGLTKNTACELGRYGIRVNCISPFGVATSMLVNAWRDDGDECMNFGIPS 240
           PHAYTASKHAIVGLTKNTACELGRYGIRVNCISPFGVATSMLVNAWRDDGDECMNFGIPS
Sbjct: 181 PHAYTASKHAIVGLTKNTACELGRYGIRVNCISPFGVATSMLVNAWRDDGDECMNFGIPS 240

Query: 241 PAEVDKMEEFVRGLANLKGPTLRPKDIAEAALYLASDESKYVSGHNLVVDGGITTSRNCI 300
           PAEVDKMEEFVRGLANLKGPTLRPKDIAEAALYLASDESKYVSGHNLVVDGGITTSRNCI
Sbjct: 241 PAEVDKMEEFVRGLANLKGPTLRPKDIAEAALYLASDESKYVSGHNLVVDGGITTSRNCI 300

Query: 301 GL 303
           GL
Sbjct: 301 GL 302

BLAST of CmaCh06G002030 vs. NCBI nr
Match: XP_022947494.1 (short-chain dehydrogenase reductase 2a [Cucurbita moschata] >KAG6596319.1 Short-chain dehydrogenase reductase 2a, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 602.1 bits (1551), Expect = 2.7e-168
Identity = 299/302 (99.01%), Postives = 301/302 (99.67%), Query Frame = 0

Query: 1   MTAQVLPEQALQSNIHLLQRDSPTPSYKRLEGKVAIVTGGAKGIGEATVRLFAKHGAKVV 60
           MTAQVLPEQALQSNIHLLQRD+P PSYKRLEGKVAIVTGGAKGIGEATVRLFAKHGAKVV
Sbjct: 1   MTAQVLPEQALQSNIHLLQRDTPAPSYKRLEGKVAIVTGGAKGIGEATVRLFAKHGAKVV 60

Query: 61  IADVEDLLGEALADTLSPNPVTFVHCDVSLEEDMENLINSTMCRHGQVDILFNNAGVLGN 120
           IADVEDLLGEALADTLSPNPV+FVHCDVSLEEDMENLINSTMCRHGQVDILFNNAGVLGN
Sbjct: 61  IADVEDLLGEALADTLSPNPVSFVHCDVSLEEDMENLINSTMCRHGQVDILFNNAGVLGN 120

Query: 121 QSKTRKSIVDFDPDEFERVMCVNVKGVALGIKHAARVMIPRATGCIISTASVAGVSGGMG 180
           QSKTRKSIVDFDPDEFERVMCVNVKGVALGIKHAARVMIPRATGCIISTASVAGVSGGMG
Sbjct: 121 QSKTRKSIVDFDPDEFERVMCVNVKGVALGIKHAARVMIPRATGCIISTASVAGVSGGMG 180

Query: 181 PHAYTASKHAIVGLTKNTACELGRYGIRVNCISPFGVATSMLVNAWRDDGDECMNFGIPS 240
           PHAYTASKHAIVGLTKNTACELGRYGIRVNCISPFGVATSMLVNAWRDDGDECMNFGIPS
Sbjct: 181 PHAYTASKHAIVGLTKNTACELGRYGIRVNCISPFGVATSMLVNAWRDDGDECMNFGIPS 240

Query: 241 PAEVDKMEEFVRGLANLKGPTLRPKDIAEAALYLASDESKYVSGHNLVVDGGITTSRNCI 300
           PAEVDKMEEFVRGLANLKGPTLRPKDIAEAALYLASDESKYVSGHNLVVDGGITTSRNCI
Sbjct: 241 PAEVDKMEEFVRGLANLKGPTLRPKDIAEAALYLASDESKYVSGHNLVVDGGITTSRNCI 300

Query: 301 GL 303
           GL
Sbjct: 301 GL 302

BLAST of CmaCh06G002030 vs. NCBI nr
Match: XP_023539546.1 (short-chain dehydrogenase reductase 2a [Cucurbita pepo subsp. pepo])

HSP 1 Score: 597.8 bits (1540), Expect = 5.1e-167
Identity = 297/301 (98.67%), Postives = 299/301 (99.34%), Query Frame = 0

Query: 1   MTAQVLPEQALQSNIHLLQRDSPTPSYKRLEGKVAIVTGGAKGIGEATVRLFAKHGAKVV 60
           MTAQVLPEQ LQSNIHLLQRDSP PSYKRLEGKVAIVTGGAKGIGEATVRLFAKHGAKVV
Sbjct: 1   MTAQVLPEQPLQSNIHLLQRDSPAPSYKRLEGKVAIVTGGAKGIGEATVRLFAKHGAKVV 60

Query: 61  IADVEDLLGEALADTLSPNPVTFVHCDVSLEEDMENLINSTMCRHGQVDILFNNAGVLGN 120
           IADVEDLLGEALADTLSPNPV+FVHCDVSLEEDMENLINSTMCRHGQVDILFNNAGVLGN
Sbjct: 61  IADVEDLLGEALADTLSPNPVSFVHCDVSLEEDMENLINSTMCRHGQVDILFNNAGVLGN 120

Query: 121 QSKTRKSIVDFDPDEFERVMCVNVKGVALGIKHAARVMIPRATGCIISTASVAGVSGGMG 180
           QSKTRKSIVDFDP+EFERVMCVNVKGVALGIKHAARVMIPRATGCIISTASVAGVSGGMG
Sbjct: 121 QSKTRKSIVDFDPNEFERVMCVNVKGVALGIKHAARVMIPRATGCIISTASVAGVSGGMG 180

Query: 181 PHAYTASKHAIVGLTKNTACELGRYGIRVNCISPFGVATSMLVNAWRDDGDECMNFGIPS 240
           PHAYTASKHAIVGLTKNTACELGRYGIRVNCISPFGVATSMLVNAWRDDGDECMNFGIPS
Sbjct: 181 PHAYTASKHAIVGLTKNTACELGRYGIRVNCISPFGVATSMLVNAWRDDGDECMNFGIPS 240

Query: 241 PAEVDKMEEFVRGLANLKGPTLRPKDIAEAALYLASDESKYVSGHNLVVDGGITTSRNCI 300
           PAEVDKMEEFVRGLANLKGPTLRPKDIAEAALYLASDESKYVSGHNLVVDGGITTSRNCI
Sbjct: 241 PAEVDKMEEFVRGLANLKGPTLRPKDIAEAALYLASDESKYVSGHNLVVDGGITTSRNCI 300

Query: 301 G 302
           G
Sbjct: 301 G 301

BLAST of CmaCh06G002030 vs. NCBI nr
Match: XP_022934318.1 (short-chain dehydrogenase reductase 2a-like [Cucurbita moschata] >KAG6580441.1 Short-chain dehydrogenase reductase 2a, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 549.7 bits (1415), Expect = 1.6e-152
Identity = 277/305 (90.82%), Postives = 290/305 (95.08%), Query Frame = 0

Query: 1   MTAQVLPEQALQSNIHLLQRDSPTPSYKRLEGKVAIVTGGAKGIGEATVRLFAKHGAKVV 60
           MTAQVLPEQALQSNIHLL RD+    +KRLEGKVAIVTGGAKGIGEATVRLFAKHGAKVV
Sbjct: 1   MTAQVLPEQALQSNIHLLGRDNSPSLHKRLEGKVAIVTGGAKGIGEATVRLFAKHGAKVV 60

Query: 61  IADVEDLLGEALADTLSPNPVTFVHCDVSLEEDMENLINSTMCRHGQVDILFNNAGVLGN 120
           IADVEDLLG+ALADTLSP+PV+FVHCDVS EED+ENLI+ST+CRHGQVDI+FNNAGVLGN
Sbjct: 61  IADVEDLLGQALADTLSPSPVSFVHCDVSSEEDVENLISSTVCRHGQVDIIFNNAGVLGN 120

Query: 121 QSKTRKSIVDFDPDEFERVMCVNVKGVALGIKHAARVMIPRATGCIISTASVAGVSGGMG 180
           QSKT KSIVDFDP+EFERVM VNVKGVALGIKHAARVMIPRATGCIISTASVAGV GG+G
Sbjct: 121 QSKTHKSIVDFDPNEFERVMRVNVKGVALGIKHAARVMIPRATGCIISTASVAGVLGGLG 180

Query: 181 PHAYTASKHAIVGLTKNTACELGRYGIRVNCISPFGVATSMLVNAWRDDG---DECMNFG 240
           PHAYTASKHAIVGLTKNTACELGRYGIRVNCISPFGVATSMLVNAWR DG   DECMN G
Sbjct: 181 PHAYTASKHAIVGLTKNTACELGRYGIRVNCISPFGVATSMLVNAWRADGDEEDECMNLG 240

Query: 241 IPSPAEVDKMEEFVRGLANLKGPTLRPKDIAEAALYLASDESKYVSGHNLVVDGGITTSR 300
           IPS AEVDKMEEFVRGL+NLKGPTLRPKDIAEAALYLASDESKY+SGHNLVVDGGITTSR
Sbjct: 241 IPSVAEVDKMEEFVRGLSNLKGPTLRPKDIAEAALYLASDESKYISGHNLVVDGGITTSR 300

Query: 301 NCIGL 303
           NCIGL
Sbjct: 301 NCIGL 305

BLAST of CmaCh06G002030 vs. NCBI nr
Match: XP_023528551.1 (short-chain dehydrogenase reductase 2a-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 548.1 bits (1411), Expect = 4.7e-152
Identity = 276/305 (90.49%), Postives = 289/305 (94.75%), Query Frame = 0

Query: 1   MTAQVLPEQALQSNIHLLQRDSPTPSYKRLEGKVAIVTGGAKGIGEATVRLFAKHGAKVV 60
           MTAQVLPEQALQSNIHLL RD+    +KRLEGKVAIVTGGAKGIGEATVRLFAKHGAKVV
Sbjct: 93  MTAQVLPEQALQSNIHLLGRDNSPSLHKRLEGKVAIVTGGAKGIGEATVRLFAKHGAKVV 152

Query: 61  IADVEDLLGEALADTLSPNPVTFVHCDVSLEEDMENLINSTMCRHGQVDILFNNAGVLGN 120
           IADVEDLLG+ALADTLSP+PV+FVHCDVS EED+ENLI+ST+CRHGQVDI+FNNAGVLGN
Sbjct: 153 IADVEDLLGQALADTLSPSPVSFVHCDVSSEEDVENLISSTVCRHGQVDIIFNNAGVLGN 212

Query: 121 QSKTRKSIVDFDPDEFERVMCVNVKGVALGIKHAARVMIPRATGCIISTASVAGVSGGMG 180
           QSKT KSIVDFDP+EFERVM VNVKGVALGIKHAARVMIPRATGCIISTASVAGV GG+G
Sbjct: 213 QSKTHKSIVDFDPNEFERVMRVNVKGVALGIKHAARVMIPRATGCIISTASVAGVLGGLG 272

Query: 181 PHAYTASKHAIVGLTKNTACELGRYGIRVNCISPFGVATSMLVNAWRDDG---DECMNFG 240
           PHAYTASKHAIVGLTKNTACELGRYGIRVNCISPFGVATSMLVNAWR DG   DECMN G
Sbjct: 273 PHAYTASKHAIVGLTKNTACELGRYGIRVNCISPFGVATSMLVNAWRADGDEEDECMNLG 332

Query: 241 IPSPAEVDKMEEFVRGLANLKGPTLRPKDIAEAALYLASDESKYVSGHNLVVDGGITTSR 300
           IPS  EVDKMEEFVRGL+NLKGPTLRPKDIAEAALYLASDESKY+SGHNLVVDGGITTSR
Sbjct: 333 IPSVGEVDKMEEFVRGLSNLKGPTLRPKDIAEAALYLASDESKYISGHNLVVDGGITTSR 392

Query: 301 NCIGL 303
           NCIGL
Sbjct: 393 NCIGL 397

BLAST of CmaCh06G002030 vs. TAIR 10
Match: AT3G51680.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 395.2 bits (1014), Expect = 4.7e-110
Identity = 208/309 (67.31%), Postives = 245/309 (79.29%), Query Frame = 0

Query: 1   MTAQVLPEQALQSNIH--LLQRDSPTPSYKRLEGKVAIVTGGAKGIGEATVRLFAKHGAK 60
           M AQV+ EQ    ++H  +++  + T   KRLEGKVAI+TGGA GIG+ATV LFA+HGA 
Sbjct: 1   MPAQVIAEQTTFHSVHDTIMEETNTTLYPKRLEGKVAIITGGAHGIGKATVMLFARHGAT 60

Query: 61  VVIADVEDLLGEALADTLSPNP----VTFVHCDVSLEEDMENLINSTMCRHGQVDILFNN 120
           VVIADV+++ G +LA +LS +     V F+ CDVS+E D+ENL+N T+ R+G++DILFNN
Sbjct: 61  VVIADVDNVAGSSLAKSLSSHKTSPMVAFISCDVSVEADVENLVNVTVARYGRLDILFNN 120

Query: 121 AGVLGNQSKTRKSIVDFDPDEFERVMCVNVKGVALGIKHAARVMIPRA-TGCIISTASVA 180
           AGVLG+Q K  KSI+DFD DEF+ VM VNV+GV LG+KH AR MI R   GCIISTASVA
Sbjct: 121 AGVLGDQKK-HKSILDFDADEFDHVMRVNVRGVGLGMKHGARAMIKRGFKGCIISTASVA 180

Query: 181 GVSGGMGPHAYTASKHAIVGLTKNTACELGRYGIRVNCISPFGVATSMLVNAWRDDGDEC 240
           GV GGMGPHAYTASKHAIVGLTKN ACELG+YGIRVNCISPFGVATSMLVNAWR      
Sbjct: 181 GVMGGMGPHAYTASKHAIVGLTKNAACELGKYGIRVNCISPFGVATSMLVNAWRKTSG-- 240

Query: 241 MNFGIPSPAEVDKMEEFVRGLANLKGPTLRPKDIAEAALYLASDESKYVSGHNLVVDGGI 300
              G     +V++MEEFVR LANLKG TLR  DIAEAALYLASDESKYV+GHNLVVDGG+
Sbjct: 241 ---GDVEDDDVEEMEEFVRSLANLKGETLRANDIAEAALYLASDESKYVNGHNLVVDGGV 300

Query: 301 TTSRNCIGL 303
           TT+RNC+GL
Sbjct: 301 TTARNCVGL 303

BLAST of CmaCh06G002030 vs. TAIR 10
Match: AT3G26770.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 247.7 bits (631), Expect = 1.2e-65
Identity = 130/271 (47.97%), Postives = 182/271 (67.16%), Query Frame = 0

Query: 24  TPSYKRLEGKVAIVTGGAKGIGEATVRLFAKHGAKVVIADVEDLLGEALADTLSPNPVTF 83
           T + K+LEGKVA++TGGA G+G+AT   F +HGA+VVIAD++   G   A  L  +   F
Sbjct: 35  TCTSKKLEGKVALITGGASGLGKATASEFLRHGARVVIADLDAETGTKTAKELG-SEAEF 94

Query: 84  VHCDVSLEEDMENLINSTMCRHGQVDILFNNAGVLGNQSKTRKSIVDFDPDEFERVMCVN 143
           V CDV++E D+   +  T+ R+G++D+++NNAG++G    T  SI   D  EFERVM +N
Sbjct: 95  VRCDVTVEADIAGAVEMTVERYGKLDVMYNNAGIVG--PMTPASISQLDMTEFERVMRIN 154

Query: 144 VKGVALGIKHAARVMIPRATGCIISTASVAGVSGGMGPHAYTASKHAIVGLTKNTACELG 203
           V GV  GIKHAA+ MIP  +GCI+ T+SVAGV+GG+ PH+YT SK    G+ K+ A EL 
Sbjct: 155 VFGVVSGIKHAAKFMIPARSGCILCTSSVAGVTGGLAPHSYTISKFTTPGIVKSAASELC 214

Query: 204 RYGIRVNCISPFGVATSMLVNAWRDDGDECMNFGIPSPAEVDKMEEFVRGLANLKGPTLR 263
            +G+R+NCISP  VAT + ++         +    P  +E +K+ E V+G+  LKG    
Sbjct: 215 EHGVRINCISPGTVATPLTLS--------YLQKVFPKVSE-EKLRETVKGMGELKGAECE 274

Query: 264 PKDIAEAALYLASDESKYVSGHNLVVDGGIT 295
             D+A+AALYLAS++ KYV+GHNLVVDGG+T
Sbjct: 275 EADVAKAALYLASNDGKYVTGHNLVVDGGMT 293

BLAST of CmaCh06G002030 vs. TAIR 10
Match: AT3G29250.2 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 247.3 bits (630), Expect = 1.6e-65
Identity = 130/267 (48.69%), Postives = 181/267 (67.79%), Query Frame = 0

Query: 29  RLEGKVAIVTGGAKGIGEATVRLFAKHGAKVVIADVEDLLGEALADTLSPNPVTFVHCDV 88
           RL+GK+AI+TGGA GIG   VRLF  HGAKVVI D+++ LG+ LA ++  +  +F  C+V
Sbjct: 5   RLDGKIAIITGGASGIGAEAVRLFTDHGAKVVIVDIQEELGQNLAVSIGLDKASFYRCNV 64

Query: 89  SLEEDMENLINSTMCRHGQVDILFNNAGVLGNQSKTRKSIVDFDPDEFERVMCVNVKGVA 148
           + E D+EN +  T+ +HG++D+LF+NAGVL    +   S++D D + F+R M VNV+G A
Sbjct: 65  TDETDVENAVKFTVEKHGKLDVLFSNAGVL----EAFGSVLDLDLEAFDRTMAVNVRGAA 124

Query: 149 LGIKHAARVMIPRAT-GCIISTASVAGVSGGMGPHAYTASKHAIVGLTKNTACELGRYGI 208
             IKHAAR M+   T G I+ T S+A   GG GPH+YTASKHA++GL ++    LG+YGI
Sbjct: 125 AFIKHAARSMVASGTRGSIVCTTSIAAEIGGPGPHSYTASKHALLGLIRSACAGLGQYGI 184

Query: 209 RVNCISPFGVATSMLVNAWRDDGDECMNFGIPSPAEVDKMEEFVRGLANLKGPTLRPKDI 268
           RVN ++P+GVAT M  +A+ ++              V  +EE+   L NLKG  L+ + I
Sbjct: 185 RVNGVAPYGVATGM-TSAYNEEA-------------VKMLEEYGEALGNLKGVVLKARHI 244

Query: 269 AEAALYLASDESKYVSGHNLVVDGGIT 295
           AEAAL+LASD+S Y+SG NLVVDGG +
Sbjct: 245 AEAALFLASDDSVYISGQNLVVDGGFS 253

BLAST of CmaCh06G002030 vs. TAIR 10
Match: AT3G29250.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 245.4 bits (625), Expect = 6.0e-65
Identity = 129/266 (48.50%), Postives = 180/266 (67.67%), Query Frame = 0

Query: 30  LEGKVAIVTGGAKGIGEATVRLFAKHGAKVVIADVEDLLGEALADTLSPNPVTFVHCDVS 89
           L+GK+AI+TGGA GIG   VRLF  HGAKVVI D+++ LG+ LA ++  +  +F  C+V+
Sbjct: 44  LDGKIAIITGGASGIGAEAVRLFTDHGAKVVIVDIQEELGQNLAVSIGLDKASFYRCNVT 103

Query: 90  LEEDMENLINSTMCRHGQVDILFNNAGVLGNQSKTRKSIVDFDPDEFERVMCVNVKGVAL 149
            E D+EN +  T+ +HG++D+LF+NAGVL    +   S++D D + F+R M VNV+G A 
Sbjct: 104 DETDVENAVKFTVEKHGKLDVLFSNAGVL----EAFGSVLDLDLEAFDRTMAVNVRGAAA 163

Query: 150 GIKHAARVMIPRAT-GCIISTASVAGVSGGMGPHAYTASKHAIVGLTKNTACELGRYGIR 209
            IKHAAR M+   T G I+ T S+A   GG GPH+YTASKHA++GL ++    LG+YGIR
Sbjct: 164 FIKHAARSMVASGTRGSIVCTTSIAAEIGGPGPHSYTASKHALLGLIRSACAGLGQYGIR 223

Query: 210 VNCISPFGVATSMLVNAWRDDGDECMNFGIPSPAEVDKMEEFVRGLANLKGPTLRPKDIA 269
           VN ++P+GVAT M  +A+ ++              V  +EE+   L NLKG  L+ + IA
Sbjct: 224 VNGVAPYGVATGM-TSAYNEEA-------------VKMLEEYGEALGNLKGVVLKARHIA 283

Query: 270 EAALYLASDESKYVSGHNLVVDGGIT 295
           EAAL+LASD+S Y+SG NLVVDGG +
Sbjct: 284 EAALFLASDDSVYISGQNLVVDGGFS 291

BLAST of CmaCh06G002030 vs. TAIR 10
Match: AT4G03140.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 242.3 bits (617), Expect = 5.1e-64
Identity = 124/268 (46.27%), Postives = 174/268 (64.93%), Query Frame = 0

Query: 28  KRLEGKVAIVTGGAKGIGEATVRLFAKHGAKVVIADVEDLLGEALADTLSPNPVTFVHCD 87
           ++LEGKVA++TGGA GIG+AT   F  HGAKV+IAD++  +G      L P+   +  CD
Sbjct: 76  RKLEGKVALITGGASGIGKATAGKFISHGAKVIIADIQPQIGRETEQELGPS-CAYFPCD 135

Query: 88  VSLEEDMENLINSTMCRHGQVDILFNNAGVLGNQSKTRKSIVDFDPDEFERVMCVNVKGV 147
           V+ E D+ N ++  +  H ++DI++NNAG+     KT  SIVD D + F++V+  NV+GV
Sbjct: 136 VTKESDIANAVDFAVSLHTKLDIMYNNAGI---PCKTPPSIVDLDLNVFDKVINTNVRGV 195

Query: 148 ALGIKHAARVMIPRATGCIISTASVAGVSGGMGPHAYTASKHAIVGLTKNTACELGRYGI 207
             GIKHAARVMIPR +G II   SV G+ GG+  H Y+ SK A++G+ ++TA EL ++ I
Sbjct: 196 MAGIKHAARVMIPRNSGSIICAGSVTGMMGGLAQHTYSVSKSAVIGIVRSTASELCKHRI 255

Query: 208 RVNCISPFGVATSMLVNAWRDDGDECMNFGIPSPAEVDKMEEFVRGLANLKGPTLRPKDI 267
           RVNCISPF + TS +++  R          I    +  ++ + V+    L G    P D+
Sbjct: 256 RVNCISPFAITTSFVMDEMRQ---------IYPGVDDSRLIQIVQSTGVLNGEVCEPTDV 315

Query: 268 AEAALYLASDESKYVSGHNLVVDGGITT 296
           A AA+YLASD+SKYV+GHNLVVDGG TT
Sbjct: 316 ANAAVYLASDDSKYVNGHNLVVDGGFTT 330

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SCU06.6e-10967.31Short-chain dehydrogenase reductase 2a OS=Arabidopsis thaliana OX=3702 GN=SDR2a ... [more]
P501604.2e-10364.38Sex determination protein tasselseed-2 OS=Zea mays OX=4577 GN=TS2 PE=2 SV=1[more]
Q94KL71.4e-6949.45Secoisolariciresinol dehydrogenase (Fragment) OS=Forsythia intermedia OX=55183 P... [more]
F1SWA03.3e-6849.28Zerumbone synthase OS=Zingiber zerumbet OX=311405 GN=ZSD1 PE=1 SV=1[more]
Q94KL89.7e-6849.82Secoisolariciresinol dehydrogenase (Fragment) OS=Podophyllum peltatum OX=35933 P... [more]
Match NameE-valueIdentityDescription
A0A6J1I6595.4e-170100.00short-chain dehydrogenase reductase 2a-like OS=Cucurbita maxima OX=3661 GN=LOC11... [more]
A0A6J1G6Z31.3e-16899.01short-chain dehydrogenase reductase 2a OS=Cucurbita moschata OX=3662 GN=LOC11145... [more]
A0A6J1F7B67.8e-15390.82short-chain dehydrogenase reductase 2a-like OS=Cucurbita moschata OX=3662 GN=LOC... [more]
A0A6J1J5H23.3e-15190.16short-chain dehydrogenase reductase 2a-like OS=Cucurbita maxima OX=3661 GN=LOC11... [more]
A0A0A0LF572.6e-14886.04Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G730750 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
XP_022971590.11.1e-169100.00short-chain dehydrogenase reductase 2a-like [Cucurbita maxima][more]
XP_022947494.12.7e-16899.01short-chain dehydrogenase reductase 2a [Cucurbita moschata] >KAG6596319.1 Short-... [more]
XP_023539546.15.1e-16798.67short-chain dehydrogenase reductase 2a [Cucurbita pepo subsp. pepo][more]
XP_022934318.11.6e-15290.82short-chain dehydrogenase reductase 2a-like [Cucurbita moschata] >KAG6580441.1 S... [more]
XP_023528551.14.7e-15290.49short-chain dehydrogenase reductase 2a-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
AT3G51680.14.7e-11067.31NAD(P)-binding Rossmann-fold superfamily protein [more]
AT3G26770.11.2e-6547.97NAD(P)-binding Rossmann-fold superfamily protein [more]
AT3G29250.21.6e-6548.69NAD(P)-binding Rossmann-fold superfamily protein [more]
AT3G29250.16.0e-6548.50NAD(P)-binding Rossmann-fold superfamily protein [more]
AT4G03140.15.1e-6446.27NAD(P)-binding Rossmann-fold superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002347Short-chain dehydrogenase/reductase SDRPRINTSPR00081GDHRDHcoord: 106..117
score: 56.51
coord: 205..222
score: 41.2
coord: 34..51
score: 51.21
coord: 158..174
score: 33.98
coord: 184..203
score: 44.24
coord: 257..277
score: 32.49
IPR002347Short-chain dehydrogenase/reductase SDRPRINTSPR00080SDRFAMILYcoord: 164..172
score: 45.22
coord: 106..117
score: 54.44
coord: 184..203
score: 38.49
NoneNo IPR availableGENE3D3.40.50.720coord: 21..300
e-value: 6.5E-80
score: 270.0
NoneNo IPR availablePFAMPF13561adh_short_C2coord: 39..294
e-value: 7.1E-60
score: 202.5
NoneNo IPR availablePIRSRPIRSR000095-1PIRSR000095-1coord: 36..230
e-value: 9.7E-17
score: 58.9
NoneNo IPR availablePIRSRPIRSR000094-3PIRSR000094-3coord: 30..216
e-value: 1.7E-8
score: 31.7
NoneNo IPR availablePANTHERPTHR431803-OXOACYL-(ACYL-CARRIER-PROTEIN) REDUCTASE (AFU_ORTHOLOGUE AFUA_6G11210)coord: 1..302
NoneNo IPR availablePANTHERPTHR43180:SF58SHORT-CHAIN DEHYDROGENASE REDUCTASE 2A-LIKEcoord: 1..302
NoneNo IPR availableCDDcd05326secoisolariciresinol-DH_like_SDR_ccoord: 29..294
e-value: 4.42927E-127
score: 360.618
IPR036291NAD(P)-binding domain superfamilySUPERFAMILY51735NAD(P)-binding Rossmann-fold domainscoord: 31..294

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh06G002030.1CmaCh06G002030.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0004316 3-oxoacyl-[acyl-carrier-protein] reductase (NADPH) activity
molecular_function GO:0016491 oxidoreductase activity