CmaCh06G001370 (gene) Cucurbita maxima (Rimu) v1.1

Overview


Name	CmaCh06G001370
Type	gene
Organism	Cucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Description	bifunctional nuclease 2-like
Location	Cma_Chr06: 704695 .. 707495 (+)
RNA-Seq Expression	CmaCh06G001370
Synteny	CmaCh06G001370

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

CCCAAATCTTTTCTCGTTCTTCTTTCTCAATTGATTCAGGGGATTGAAACAATCTCTTTCTTCTCTCTCTCTCAAACACTTCACGTTTCCCTTTCAATCTTCTTCATCTTCGTCTTTAATCTCCACTTTTCTCTGTAATTCCGCCATTACAATGCTGGGTCTAACCTTTCACTTCTGTGTTCCCTCCATTTTCCCCCGATCCGCCATAGCCATGGATCACCCCTCTACTTCTCGCCCTTCCTCCTCTTTCCTCCACTTCTCTCTCCCTCGATCGCCTTCCATTGCTCTTCGCTCTCGCCGCTCCAACTCGATACTCATTTCCTGCCACTCTTCTCCCGATGGATTCGCCCACACTGATGATGACTCCCATCGGGATTATTTTGAAGCTTCGTTTCTCGTCACAGGTTCTTAATCTTTCACTTGGCTACTTTTCTGTTTTACTTTGATGTTTGTTGCAATTTCCGATCGAATCGATGGGGTGTTCGTGATTTCTGTGTTTGTTTGTGCATTACTGACTATTAGAGACTATCAATCACTATCGAATGTGGAAGAAGAGGTTTCAAGAAGATGCGAGTTTCAATCAGAGAGAATCGCAAGTCAATTCCCATTCACTTGGATTGGGGTTTTTTAGACGTTTTCGAAGCCCTACTGTGTTTCTCAAGATTTCTTGCAATGGTGACTTTCTACTGCCAATTGTCGTAGGTATGTTGGACAAAAACTGTGACGTGATTGGGTTTTGTGTTTTCTTATGAATTTGTCAAAAAGAAGCTGCAAATTTTGGACTTGGTTTGCAGGGGAATATGCTATTGAGAAACTTATAGATTGTCAGCTTGGGATTGAAAATGGGGTGGGTTTTCTCTGGTTTTGTTCTTCATAAATATATATATACATATATGAATACCTACCTGTTGGTATTAATTCTTAAATGGTCACATATTACAGGATGCCCCTGATCTCTTCCAGTTTATACAAGACCTCATTGTGAAAGTTGGCTATCAAGTATGAACTGACTTCTAACTTACTGAATAAATATATGCTTAGGATTTATTGTTTATCAGTCTTTGATGGGGAGGATTTGTGAAACAAATTTATATTTTTTTTTGAGAGTTCAATAGGGGTTTAAACTCTTGACCTCTTAGTCGAAGATATATGTCTTAACTAGTTGAGCGATGCTCGAGCTAACGTTTGTGACATTAATGTTAAGAACATTAGGATGGAGTTTTTTTTTTTTTTTTTCAGATTTCTATTCTGTACTAGCTTGAAAAGTGAATTGTTGCTTGCTACTATCATACATGGAAGAATCAAGAAATATATACATGGCTTGTTAGTTTTTTCTATACAAGAACCAATGAGCTGCAAAAATCTGCTAGTTTTAACGTTGCTTTGCGATGTATGTTTACCTGATAGGACAAGCTTATGCTCGAGCAAGAATATTAGTGTCTTTGCCCATTTGTGAGATCCCACATCCGTTGGAGAGGAGAATGAAACATTCTTTATAAGGGTGTGGAAACCTCTCCCTAGCAGATGCGTTTTAGAATTGTGAGGCTGACAGCGATACATAACGGGCTAAAACAGACATCGGGCAATGTGCTAGCAAGGACGTTGGGCTCCCAAGGAGGGTGAATTACTAGATTCCACATCAGTTGGAGAGGGGAACGAAGCATTCCTCATAAGAGTGTGGAAACCTCTCCCTAACAGATGCGTTTTAAAATTGTGAGGCTGACGGTGATACGTAACAGGCGAAAGTAGACAATATCTGCTAACGGTGGACTTGGGCTGTTACACTTGCTTTCGAGGAATTATATAATTTGGGATTTAATTCTTCATCTAGTCATAGAATTATTTTCTCTAAGCTAGATTATGGGTAGAAGCACTGCACAGTTTCTGAATAAATCATTTGTACTTCTCATGTTCATTTCCTTTTTTGATAACCATGAAAAGGGGTTTAGAGCGATAATCTAATGCCATTTTATATTATCACGAGATAGGTCACCACAGCGAGAATTACTGAACGAGTGATGAACACTTACTTCGCCCGGCTCTTCTTGAAAAAGGTTAGCTCTTAATAAACTGATTCACAATCAGCCAATACCTTTTTAGAAGTTCTCAATACATGCCTTGTCTAACTATTATCATCTGCAGGATGGGGAATATGAAACGTTAAGTGTGGATGCACGCCCCTCAGACGCCCTAAACGTTGCATATCGATGTAAGGTAGATATTCTACCATTAAATGCTGGGATCCTTGCAGAACTTCTGCTAGTCGCTAGAATGACTTAGATTCTTTACTTTGCACCCTCTCCGCACGATTTCATTCTCTTGATCGAGATAATTTCTTACTTCACTGAAAGTTTGCTGCTCACGGTGTTTTTCGGTATAACAAATGTGCAGATACCTGTACTTGTTAGTAAGCAGATTGTCTATGAGGATGCCATTAGAGTTAGTTATGGGTTTGGAAGAGTGCGTGAGAGGAAATCATGTTACGACATTCTACTCGACAGGTAGAAGTGCATGTATTGTTTGCTATTTGATGAAGATCTTTTCTTGATATCCAAAAACAATCATGTTCGTTTCGATCAGTGCTGCTGATGGTCCGAATTTCTTGTCCGAGGAACTCGATATGCTGAAGAATATGAAAATGGCTATTTATGAAGAACGGTACAAAGATGCAGGTATCTATCCAAACATTTTGTTCTTTATCATGTTTCTACAAATGAGATATTTATAGCTCATGTTTGTTTCCTTCAGCTGTGTGGAGGGATAAGTTAACAAAGCTTCGCAAGTCAGAACACGAGGAGTAA

mRNA sequence

CCCAAATCTTTTCTCGTTCTTCTTTCTCAATTGATTCAGGGGATTGAAACAATCTCTTTCTTCTCTCTCTCTCAAACACTTCACGTTTCCCTTTCAATCTTCTTCATCTTCGTCTTTAATCTCCACTTTTCTCTGTAATTCCGCCATTACAATGCTGGGTCTAACCTTTCACTTCTGTGTTCCCTCCATTTTCCCCCGATCCGCCATAGCCATGGATCACCCCTCTACTTCTCGCCCTTCCTCCTCTTTCCTCCACTTCTCTCTCCCTCGATCGCCTTCCATTGCTCTTCGCTCTCGCCGCTCCAACTCGATACTCATTTCCTGCCACTCTTCTCCCGATGGATTCGCCCACACTGATGATGACTCCCATCGGGATTATTTTGAAGCTTCGTTTCTCGTCACAGAGACTATCAATCACTATCGAATGTGGAAGAAGAGGTTTCAAGAAGATGCGAGTTTCAATCAGAGAGAATCGCAAGTCAATTCCCATTCACTTGGATTGGGGTTTTTTAGACGTTTTCGAAGCCCTACTGTGTTTCTCAAGATTTCTTGCAATGGTGACTTTCTACTGCCAATTGTCGTAGGGGAATATGCTATTGAGAAACTTATAGATTGTCAGCTTGGGATTGAAAATGGGGATGCCCCTGATCTCTTCCAGTTTATACAAGACCTCATTGTGAAAGTTGGCTATCAAGTCACCACAGCGAGAATTACTGAACGAGTGATGAACACTTACTTCGCCCGGCTCTTCTTGAAAAAGGATGGGGAATATGAAACGTTAAGTGTGGATGCACGCCCCTCAGACGCCCTAAACGTTGCATATCGATGTAAGATACCTGTACTTGTTAGTAAGCAGATTGTCTATGAGGATGCCATTAGAGTTAGTTATGGGTTTGGAAGAGTGCGTGAGAGGAAATCATGTTACGACATTCTACTCGACAGTGCTGCTGATGGTCCGAATTTCTTGTCCGAGGAACTCGATATGCTGAAGAATATGAAAATGGCTATTTATGAAGAACGGTACAAAGATGCAGCTGTGTGGAGGGATAAGTTAACAAAGCTTCGCAAGTCAGAACACGAGGAGTAA

Coding sequence (CDS)

ATGCTGGGTCTAACCTTTCACTTCTGTGTTCCCTCCATTTTCCCCCGATCCGCCATAGCCATGGATCACCCCTCTACTTCTCGCCCTTCCTCCTCTTTCCTCCACTTCTCTCTCCCTCGATCGCCTTCCATTGCTCTTCGCTCTCGCCGCTCCAACTCGATACTCATTTCCTGCCACTCTTCTCCCGATGGATTCGCCCACACTGATGATGACTCCCATCGGGATTATTTTGAAGCTTCGTTTCTCGTCACAGAGACTATCAATCACTATCGAATGTGGAAGAAGAGGTTTCAAGAAGATGCGAGTTTCAATCAGAGAGAATCGCAAGTCAATTCCCATTCACTTGGATTGGGGTTTTTTAGACGTTTTCGAAGCCCTACTGTGTTTCTCAAGATTTCTTGCAATGGTGACTTTCTACTGCCAATTGTCGTAGGGGAATATGCTATTGAGAAACTTATAGATTGTCAGCTTGGGATTGAAAATGGGGATGCCCCTGATCTCTTCCAGTTTATACAAGACCTCATTGTGAAAGTTGGCTATCAAGTCACCACAGCGAGAATTACTGAACGAGTGATGAACACTTACTTCGCCCGGCTCTTCTTGAAAAAGGATGGGGAATATGAAACGTTAAGTGTGGATGCACGCCCCTCAGACGCCCTAAACGTTGCATATCGATGTAAGATACCTGTACTTGTTAGTAAGCAGATTGTCTATGAGGATGCCATTAGAGTTAGTTATGGGTTTGGAAGAGTGCGTGAGAGGAAATCATGTTACGACATTCTACTCGACAGTGCTGCTGATGGTCCGAATTTCTTGTCCGAGGAACTCGATATGCTGAAGAATATGAAAATGGCTATTTATGAAGAACGGTACAAAGATGCAGCTGTGTGGAGGGATAAGTTAACAAAGCTTCGCAAGTCAGAACACGAGGAGTAA

Protein sequence

MLGLTFHFCVPSIFPRSAIAMDHPSTSRPSSSFLHFSLPRSPSIALRSRRSNSILISCHSSPDGFAHTDDDSHRDYFEASFLVTETINHYRMWKKRFQEDASFNQRESQVNSHSLGLGFFRRFRSPTVFLKISCNGDFLLPIVVGEYAIEKLIDCQLGIENGDAPDLFQFIQDLIVKVGYQVTTARITERVMNTYFARLFLKKDGEYETLSVDARPSDALNVAYRCKIPVLVSKQIVYEDAIRVSYGFGRVRERKSCYDILLDSAADGPNFLSEELDMLKNMKMAIYEERYKDAAVWRDKLTKLRKSEHEE

Homology

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BLAST of CmaCh06G001370 vs. ExPASy Swiss-Prot
Match: Q93VH2 (Bifunctional nuclease 2 OS=Arabidopsis thaliana OX=3702 GN=BBD2 PE=2 SV=1)

HSP 1 Score: 132.1 bits (331), Expect = 1.1e-29
Identity = 87/266 (32.71%), Postives = 139/266 (52.26%), Query Frame = 0


Query: 46  LRSRRSNSILISCHSSPDG-FAHTDDDSHRDYFEASFLVTETINHYRMWKKRFQEDASFN 105
           LR  +S   L S HS   G  A   +++  DY ++S L    +   +     F       
Sbjct: 60  LRRYKSIKCLFSSHSDGTGSTAENFNENDEDYVKSSVLEAVEV---KSGPDGFMVKMKDG 119

Query: 106 QRESQVNSHSLGLGFFRRFRSPTVFLKISCNGDFLLPIVVGEYAIEKLIDCQLGIENGDA 165
           ++   V+++  G           + LK+      LLPI+V E     L+     ++    
Sbjct: 120 RQLRCVHNNPQGGNLPNYAPHSAIVLKMEDGTGLLLPIIVLEMPSVLLMAAMTNVQIA-R 179

Query: 166 PDLFQFIQDLIVKVGYQVTTARITERVMNTYFARLFLKKDGE-YETLSVDARPSDALNVA 225
           P ++Q ++D++ K+GY+V   R+T RV   YFA L+L K G+  + +S D RPSDA+N+A
Sbjct: 180 PTMYQVVKDMVDKMGYEVRLVRVTTRVHEAYFAELYLSKVGDKSDCVSFDLRPSDAINIA 239

Query: 226 YRCKIPVLVSKQIVYEDAIRVSYGFGRVRERKSCYDILLDSAADGPN----FLSEELDML 285
            RCK+P+ V+K + Y D +RV    G++ ++    D LL +  D PN    F ++E D++
Sbjct: 240 VRCKVPIQVNKYLAYSDGMRV-IDSGKLSKQTPASDGLLFTELDRPNGQPCFDTKEFDLV 299

Query: 286 KNMKMAIYEERYKDAAVWRDKLTKLR 306
           +NM  A+ EERY +AA WRDKL K +
Sbjct: 300 RNMMQAVDEERYDEAAEWRDKLGKFQ 320

[Close]

BLAST of CmaCh06G001370 vs. ExPASy Swiss-Prot
Match: Q9FWS6 (Bifunctional nuclease 1 OS=Arabidopsis thaliana OX=3702 GN=BBD1 PE=2 SV=1)

HSP 1 Score: 127.9 bits (320), Expect = 2.0e-28
Identity = 72/185 (38.92%), Postives = 107/185 (57.84%), Query Frame = 0


Query: 126 PTVFLKISCNGDFLLPIVVGEYAIEKLIDCQLGIENGDAPDLFQFIQDLIVKVGYQVTTA 185
           P + LK+      LLPI+V E     L+     ++    P ++Q +++++ K+GY+V   
Sbjct: 134 PAIVLKMEDGTGLLLPIIVLEMPSVLLMAAMTNVQIA-RPTMYQVVKEMVDKMGYEVRLV 193

Query: 186 RITERVMNTYFARLFLKKDGE-YETLSVDARPSDALNVAYRCKIPVLVSKQIVYEDAIRV 245
           R+T+RV   YFA+LFL K G   E +S D RPSDA+N+A RCKIP+ V+K + Y D +RV
Sbjct: 194 RVTKRVHEAYFAQLFLSKVGNASECVSFDLRPSDAINIAVRCKIPIQVNKYLAYSDGMRV 253

Query: 246 SYGFGRVRERKSCYDILLDSAADGPN----FLSEELDMLKNMKMAIYEERYKDAAVWRDK 305
               G++       D LL +  D PN      ++E ++L  M  A+ EERY +AA WRDK
Sbjct: 254 IES-GKISTPAPASDGLLFTEQDRPNGQACLDTKEFNILSKMMQAVDEERYDEAAEWRDK 313

[Close]

BLAST of CmaCh06G001370 vs. ExPASy Swiss-Prot
Match: Q6YZM6 (Bifunctional nuclease 2 OS=Oryza sativa subsp. japonica OX=39947 GN=BBD2 PE=2 SV=1)

HSP 1 Score: 126.7 bits (317), Expect = 4.5e-28
Identity = 74/201 (36.82%), Postives = 113/201 (56.22%), Query Frame = 0


Query: 110 VNSHSLGLGFFRRFRSPTVFLKISCNGDFLLPIVVGEYAIEKLIDCQLGIENGDAPDLFQ 169
           V+++S G         P + L+I    + LLPI+V E     L+     +     P ++Q
Sbjct: 120 VHNNSQGRNIPESAPQPAIVLRIEDGSETLLPIIVLEMPSVLLMAAIRNVHIA-RPTIYQ 179

Query: 170 FIQDLIVKVGYQVTTARITERVMNTYFARLFLKKDGEY-ETLSVDARPSDALNVAYRCKI 229
            ++++I K+GY+V   RI +R+   Y A LFL K G++ E+++ D RPSDA+N+A RCK+
Sbjct: 180 VVKEMIDKMGYEVKLVRINKRIQEAYCAELFLTKVGDHTESITFDLRPSDAINIAVRCKV 239

Query: 230 PVLVSKQIVYEDAIRVSYGFGRVRERKSCYDILLDSAADGPN----FLSEELDMLKNMKM 289
           P+ V + + Y D IR S    R+       D LL +  D P+      ++E  +++NM +
Sbjct: 240 PIQVHRSLAYSDGIR-SVEPARMAIAAGMSDGLLFTELDRPDGQPCVEAQEFGLIRNMLI 299

Query: 290 AIYEERYKDAAVWRDKLTKLR 306
           A  EERYKDAA WRDKL  LR
Sbjct: 300 AAVEERYKDAATWRDKLMLLR 318

[Close]

BLAST of CmaCh06G001370 vs. ExPASy Swiss-Prot
Match: Q5N8J3 (Bifunctional nuclease 1 OS=Oryza sativa subsp. japonica OX=39947 GN=BBD1 PE=2 SV=1)

HSP 1 Score: 121.7 bits (304), Expect = 1.5e-26
Identity = 69/182 (37.91%), Postives = 109/182 (59.89%), Query Frame = 0


Query: 128 VFLKISCNGDFLLPIVVGEYAIEKLIDCQLGIENGDAPDLFQFIQDLIVKVGYQVTTARI 187
           + LK+    D LLPI+V E     L+     I     P ++  ++++  ++GY V   RI
Sbjct: 145 IVLKMEDGSDLLLPIIVMETPSIMLLAALRNIRI-PRPTIYNVVKEMTERMGYAVRLVRI 204

Query: 188 TERVMNTYFARLFLKKDG-EYETLSVDARPSDALNVAYRCKIPVLVSKQIVYEDAIRV-- 247
           TE V + Y++RL+L K G E ET+S+D +PSDA+N+A+RCK+P+ V+++I Y + ++V  
Sbjct: 205 TEMVHDAYYSRLYLAKIGNEEETISLDLKPSDAINIAFRCKVPIQVNRRIAYNNGLKVVQ 264

Query: 248 -SYGFGRVRERKSCYDILLDSAADGPNFLSEELDMLKNMKMAIYEERYKDAAVWRDKLTK 306
            +     V   +  Y   LD   D P F ++E D+++NM +A  EERYKDAA +RD+L  
Sbjct: 265 PTPSESYVSSDQFQY-TRLDRPDDQPCFEAQEFDLVRNMLVAAVEERYKDAAQYRDQLFM 324

[Close]

BLAST of CmaCh06G001370 vs. ExPASy Swiss-Prot
Match: Q09LL3 (Bifunctional nuclease OS=Oryza minuta OX=63629 GN=BBD PE=2 SV=1)

HSP 1 Score: 121.7 bits (304), Expect = 1.5e-26
Identity = 69/182 (37.91%), Postives = 109/182 (59.89%), Query Frame = 0


Query: 128 VFLKISCNGDFLLPIVVGEYAIEKLIDCQLGIENGDAPDLFQFIQDLIVKVGYQVTTARI 187
           + LK+    D LLPI+V E     L+     I     P ++  ++++  ++GY V   RI
Sbjct: 145 IVLKMEDGSDLLLPIIVMETPSIMLLAALRNIRI-PRPTIYNVVKEMTERMGYAVRLVRI 204

Query: 188 TERVMNTYFARLFLKKDG-EYETLSVDARPSDALNVAYRCKIPVLVSKQIVYEDAIRV-- 247
           TE V + Y++RL+L K G E ET+S+D +PSDA+N+A+RCK+P+ V+++I Y + ++V  
Sbjct: 205 TEMVHDAYYSRLYLAKIGNEEETISLDLKPSDAINIAFRCKVPIQVNRRIAYNNGLKVVQ 264

Query: 248 -SYGFGRVRERKSCYDILLDSAADGPNFLSEELDMLKNMKMAIYEERYKDAAVWRDKLTK 306
            +     V   +  Y   LD   D P F ++E D+++NM +A  EERYKDAA +RD+L  
Sbjct: 265 PTPSESYVSSDQFQY-TRLDRPDDQPCFEAQEFDLVRNMLVAAVEERYKDAAQYRDQLFM 324

[Close]

BLAST of CmaCh06G001370 vs. ExPASy TrEMBL
Match: A0A6J1I8T8 (bifunctional nuclease 2-like OS=Cucurbita maxima OX=3661 GN=LOC111470219 PE=3 SV=1)

HSP 1 Score: 625.5 bits (1612), Expect = 1.1e-175
Identity = 311/311 (100.00%), Postives = 311/311 (100.00%), Query Frame = 0


Query: 1   MLGLTFHFCVPSIFPRSAIAMDHPSTSRPSSSFLHFSLPRSPSIALRSRRSNSILISCHS 60
           MLGLTFHFCVPSIFPRSAIAMDHPSTSRPSSSFLHFSLPRSPSIALRSRRSNSILISCHS
Sbjct: 1   MLGLTFHFCVPSIFPRSAIAMDHPSTSRPSSSFLHFSLPRSPSIALRSRRSNSILISCHS 60

Query: 61  SPDGFAHTDDDSHRDYFEASFLVTETINHYRMWKKRFQEDASFNQRESQVNSHSLGLGFF 120
           SPDGFAHTDDDSHRDYFEASFLVTETINHYRMWKKRFQEDASFNQRESQVNSHSLGLGFF
Sbjct: 61  SPDGFAHTDDDSHRDYFEASFLVTETINHYRMWKKRFQEDASFNQRESQVNSHSLGLGFF 120

Query: 121 RRFRSPTVFLKISCNGDFLLPIVVGEYAIEKLIDCQLGIENGDAPDLFQFIQDLIVKVGY 180
           RRFRSPTVFLKISCNGDFLLPIVVGEYAIEKLIDCQLGIENGDAPDLFQFIQDLIVKVGY
Sbjct: 121 RRFRSPTVFLKISCNGDFLLPIVVGEYAIEKLIDCQLGIENGDAPDLFQFIQDLIVKVGY 180

Query: 181 QVTTARITERVMNTYFARLFLKKDGEYETLSVDARPSDALNVAYRCKIPVLVSKQIVYED 240
           QVTTARITERVMNTYFARLFLKKDGEYETLSVDARPSDALNVAYRCKIPVLVSKQIVYED
Sbjct: 181 QVTTARITERVMNTYFARLFLKKDGEYETLSVDARPSDALNVAYRCKIPVLVSKQIVYED 240

Query: 241 AIRVSYGFGRVRERKSCYDILLDSAADGPNFLSEELDMLKNMKMAIYEERYKDAAVWRDK 300
           AIRVSYGFGRVRERKSCYDILLDSAADGPNFLSEELDMLKNMKMAIYEERYKDAAVWRDK
Sbjct: 241 AIRVSYGFGRVRERKSCYDILLDSAADGPNFLSEELDMLKNMKMAIYEERYKDAAVWRDK 300

Query: 301 LTKLRKSEHEE 312
           LTKLRKSEHEE
Sbjct: 301 LTKLRKSEHEE 311

[Close]

BLAST of CmaCh06G001370 vs. ExPASy TrEMBL
Match: A0A6J1GHK4 (bifunctional nuclease 2 OS=Cucurbita moschata OX=3662 GN=LOC111454246 PE=3 SV=1)

HSP 1 Score: 582.4 bits (1500), Expect = 1.1e-162
Identity = 295/311 (94.86%), Postives = 299/311 (96.14%), Query Frame = 0


Query: 1   MLGLTFHFCVPSIFPRSAIAMDHPSTSRPSSSFLHFSLPRSPSIALRSRRSNSILISCHS 60
           MLGLTFHFCVPSIFPRSAIAMDHPST    SS LHFSL RS SIA RSRRSNSILISCHS
Sbjct: 1   MLGLTFHFCVPSIFPRSAIAMDHPST----SSSLHFSLLRSRSIAFRSRRSNSILISCHS 60

Query: 61  SPDGFAHTDDDSHRDYFEASFLVTETINHYRMWKKRFQEDASFNQRESQVNSHSLGLGFF 120
           SPD FAHTDDDS RDYFEASFLVTETINHYRMWKKRFQEDASFNQRES+VNSHSLGLGFF
Sbjct: 61  SPDRFAHTDDDSRRDYFEASFLVTETINHYRMWKKRFQEDASFNQRESRVNSHSLGLGFF 120

Query: 121 RRFRSPTVFLKISCNGDFLLPIVVGEYAIEKLIDCQLGIENGDAPDLFQFIQDLIVKVGY 180
           RRFRSPTVFLKISCNGDFLLPIVVGEYAIEKLIDCQLGIENGDAPDLFQFIQDLIVKVGY
Sbjct: 121 RRFRSPTVFLKISCNGDFLLPIVVGEYAIEKLIDCQLGIENGDAPDLFQFIQDLIVKVGY 180

Query: 181 QVTTARITERVMNTYFARLFLKKDGEYETLSVDARPSDALNVAYRCKIPVLVSKQIVYED 240
           QVTTARITERVMNTYFARLFLKKDGE ETLSVDARPSDALNVAYRCKIPVLVSKQIVYED
Sbjct: 181 QVTTARITERVMNTYFARLFLKKDGENETLSVDARPSDALNVAYRCKIPVLVSKQIVYED 240

Query: 241 AIRVSYGFGRVRERKSCYDILLDSAADGPNFLSEELDMLKNMKMAIYEERYKDAAVWRDK 300
           AIRVSYGFGRVRERKSCYD+LLDSAADGP+FLSEELDMLKNMKMAIYEERYKDAA+WRDK
Sbjct: 241 AIRVSYGFGRVRERKSCYDVLLDSAADGPDFLSEELDMLKNMKMAIYEERYKDAALWRDK 300

Query: 301 LTKLRKSEHEE 312
           LTKLRKSE EE
Sbjct: 301 LTKLRKSEDEE 307

[Close]

BLAST of CmaCh06G001370 vs. ExPASy TrEMBL
Match: A0A0A0L3L8 (BFN domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G002490 PE=3 SV=1)

HSP 1 Score: 518.1 bits (1333), Expect = 2.6e-143
Identity = 262/312 (83.97%), Postives = 284/312 (91.03%), Query Frame = 0


Query: 1   MLGLTFHFCVPSIFPRSAIAMDHPSTSRPSSSFLHFSLPRSPSIALRSRRSNSILISCHS 60
           MLGL  HFC+P+IF  SAIAMDHPS SRPS S LHFSL RS   A+RSRR  SILISCHS
Sbjct: 1   MLGLAPHFCLPTIFFGSAIAMDHPSASRPSCS-LHFSLHRSRPNAVRSRRPKSILISCHS 60

Query: 61  SPDGFAHTDDD--SHRDYFEASFLVTETINHYRMWKKRFQEDASFNQRESQVNSHSLGLG 120
           S D   H+DDD  SH+DYFEASFLV+ETI HYRMWKKRFQEDA+FNQRES+ NS+SLGLG
Sbjct: 61  SRDRSTHSDDDDNSHQDYFEASFLVSETIYHYRMWKKRFQEDANFNQRESRANSNSLGLG 120

Query: 121 FFRRFRSPTVFLKISCNGDFLLPIVVGEYAIEKLIDCQLGIENGDAPDLFQFIQDLIVKV 180
           FFRRFRSPTVFLKISCNGDFLLPIVVGEYAIEKLIDCQLGIENG+APD+FQFIQDLIVKV
Sbjct: 121 FFRRFRSPTVFLKISCNGDFLLPIVVGEYAIEKLIDCQLGIENGEAPDIFQFIQDLIVKV 180

Query: 181 GYQVTTARITERVMNTYFARLFLKKDGEYETLSVDARPSDALNVAYRCKIPVLVSKQIVY 240
           GY+  TARITERV+NTYFARLFL+K+GE E LSVDARPSDALN+AYRCKIPVLVSKQIV+
Sbjct: 181 GYEAITARITERVVNTYFARLFLRKEGESEMLSVDARPSDALNIAYRCKIPVLVSKQIVF 240

Query: 241 EDAIRVSYGFGRVRERKSCYDILLDSAADGPNFLSEELDMLKNMKMAIYEERYKDAAVWR 300
           EDAIRVSYGFGRV ERKSC+D+LLD AADGP+FLSEELDMLKNMK+AIYEERYKDAA+WR
Sbjct: 241 EDAIRVSYGFGRVHERKSCFDVLLDCAADGPDFLSEELDMLKNMKIAIYEERYKDAAMWR 300

Query: 301 DKLTKLRKSEHE 311
           DKLTKLRKS HE
Sbjct: 301 DKLTKLRKSVHE 311

[Close]

BLAST of CmaCh06G001370 vs. ExPASy TrEMBL
Match: A0A6J1HJX9 (bifunctional nuclease 2-like OS=Cucurbita maxima OX=3661 GN=LOC111465202 PE=3 SV=1)

HSP 1 Score: 516.9 bits (1330), Expect = 5.8e-143
Identity = 260/310 (83.87%), Postives = 284/310 (91.61%), Query Frame = 0


Query: 1   MLGLTFHFCVPSIFPRSAIAMDHPSTSRPSSSFLHFSLPRSPSIALRSRRSNSILISCHS 60
           MLGL  HF + ++FP SAIAMDHPS SRPSSS LHFS+ RS   A+RSRR  SIL+SCHS
Sbjct: 1   MLGLVPHFSLRTLFPGSAIAMDHPSASRPSSS-LHFSVLRSRPNAVRSRRPKSILVSCHS 60

Query: 61  SPDGFAHTDDDSHRDYFEASFLVTETINHYRMWKKRFQEDASFNQRESQVNSHSLGLGFF 120
           S D   H+DDDSH+DYFEASFLV+ETI HYRMWKKRFQED +FNQ ES+VNS SLGLGFF
Sbjct: 61  SRDRSTHSDDDSHQDYFEASFLVSETIYHYRMWKKRFQEDTNFNQIESRVNSPSLGLGFF 120

Query: 121 RRFRSPTVFLKISCNGDFLLPIVVGEYAIEKLIDCQLGIENGDAPDLFQFIQDLIVKVGY 180
           RRF+SPTVFLKISCNGDFLLPIVVGEYAIEKLID  LGIENG+APDLFQFIQDL+VKVGY
Sbjct: 121 RRFQSPTVFLKISCNGDFLLPIVVGEYAIEKLIDSHLGIENGEAPDLFQFIQDLMVKVGY 180

Query: 181 QVTTARITERVMNTYFARLFLKKDGEYETLSVDARPSDALNVAYRCKIPVLVSKQIVYED 240
           +VTTARITERV+NTYFARLFL+KDGE ETLSVDARPSDALN+AYRCKIPVLVSKQIVYED
Sbjct: 181 EVTTARITERVVNTYFARLFLRKDGENETLSVDARPSDALNIAYRCKIPVLVSKQIVYED 240

Query: 241 AIRVSYGFGRVRERKSCYDILLDSAADGPNFLSEELDMLKNMKMAIYEERYKDAAVWRDK 300
           AIRVS+GFGRVR+RKSC+D+LLD AADGP+FLSEELDMLKNMKMAIYEERYKDAA+WRDK
Sbjct: 241 AIRVSFGFGRVRKRKSCFDVLLDCAADGPDFLSEELDMLKNMKMAIYEERYKDAAMWRDK 300

Query: 301 LTKLRKSEHE 311
           LTKLRKS HE
Sbjct: 301 LTKLRKSVHE 309

[Close]

BLAST of CmaCh06G001370 vs. ExPASy TrEMBL
Match: A0A6J1E735 (bifunctional nuclease 2-like OS=Cucurbita moschata OX=3662 GN=LOC111431208 PE=3 SV=1)

HSP 1 Score: 514.2 bits (1323), Expect = 3.7e-142
Identity = 259/310 (83.55%), Postives = 283/310 (91.29%), Query Frame = 0


Query: 1   MLGLTFHFCVPSIFPRSAIAMDHPSTSRPSSSFLHFSLPRSPSIALRSRRSNSILISCHS 60
           MLGL  HF + ++FP SAIAMDHPS SRPSSS LHFS+ RS   A+ SRR  SIL+SCHS
Sbjct: 1   MLGLAPHFSLRTLFPGSAIAMDHPSASRPSSS-LHFSVLRSRPNAVSSRRPKSILVSCHS 60

Query: 61  SPDGFAHTDDDSHRDYFEASFLVTETINHYRMWKKRFQEDASFNQRESQVNSHSLGLGFF 120
           S D   H+DDDSH+DYFEASFLV+ETI HYRMWKKRFQEDA+FNQ ES+VNS  LGLGFF
Sbjct: 61  SRDRSTHSDDDSHQDYFEASFLVSETIYHYRMWKKRFQEDANFNQIESRVNSPLLGLGFF 120

Query: 121 RRFRSPTVFLKISCNGDFLLPIVVGEYAIEKLIDCQLGIENGDAPDLFQFIQDLIVKVGY 180
           RRFRSPTVFLKISCNGDFLLPIVVGEYAIEKLID  LGIENG+APDLFQFIQDL+VKVGY
Sbjct: 121 RRFRSPTVFLKISCNGDFLLPIVVGEYAIEKLIDSHLGIENGEAPDLFQFIQDLMVKVGY 180

Query: 181 QVTTARITERVMNTYFARLFLKKDGEYETLSVDARPSDALNVAYRCKIPVLVSKQIVYED 240
           +VTTARITERV+NTYFARLFL+KDGE ETLSVDARPSDALN+AYRCKIPVLVSKQIVYED
Sbjct: 181 EVTTARITERVVNTYFARLFLRKDGENETLSVDARPSDALNIAYRCKIPVLVSKQIVYED 240

Query: 241 AIRVSYGFGRVRERKSCYDILLDSAADGPNFLSEELDMLKNMKMAIYEERYKDAAVWRDK 300
           AIRVS+GFGRVR+RKSC+D+LLD AADGP+FLSEELDMLKNMKMAIYEERYKDAA+WRDK
Sbjct: 241 AIRVSFGFGRVRKRKSCFDVLLDCAADGPDFLSEELDMLKNMKMAIYEERYKDAAMWRDK 300

Query: 301 LTKLRKSEHE 311
           LT+LRKS HE
Sbjct: 301 LTRLRKSVHE 309

[Close]

BLAST of CmaCh06G001370 vs. NCBI nr
Match: XP_022971524.1 (bifunctional nuclease 2-like [Cucurbita maxima])

HSP 1 Score: 625.5 bits (1612), Expect = 2.4e-175
Identity = 311/311 (100.00%), Postives = 311/311 (100.00%), Query Frame = 0


Query: 1   MLGLTFHFCVPSIFPRSAIAMDHPSTSRPSSSFLHFSLPRSPSIALRSRRSNSILISCHS 60
           MLGLTFHFCVPSIFPRSAIAMDHPSTSRPSSSFLHFSLPRSPSIALRSRRSNSILISCHS
Sbjct: 1   MLGLTFHFCVPSIFPRSAIAMDHPSTSRPSSSFLHFSLPRSPSIALRSRRSNSILISCHS 60

Query: 61  SPDGFAHTDDDSHRDYFEASFLVTETINHYRMWKKRFQEDASFNQRESQVNSHSLGLGFF 120
           SPDGFAHTDDDSHRDYFEASFLVTETINHYRMWKKRFQEDASFNQRESQVNSHSLGLGFF
Sbjct: 61  SPDGFAHTDDDSHRDYFEASFLVTETINHYRMWKKRFQEDASFNQRESQVNSHSLGLGFF 120

Query: 121 RRFRSPTVFLKISCNGDFLLPIVVGEYAIEKLIDCQLGIENGDAPDLFQFIQDLIVKVGY 180
           RRFRSPTVFLKISCNGDFLLPIVVGEYAIEKLIDCQLGIENGDAPDLFQFIQDLIVKVGY
Sbjct: 121 RRFRSPTVFLKISCNGDFLLPIVVGEYAIEKLIDCQLGIENGDAPDLFQFIQDLIVKVGY 180

Query: 181 QVTTARITERVMNTYFARLFLKKDGEYETLSVDARPSDALNVAYRCKIPVLVSKQIVYED 240
           QVTTARITERVMNTYFARLFLKKDGEYETLSVDARPSDALNVAYRCKIPVLVSKQIVYED
Sbjct: 181 QVTTARITERVMNTYFARLFLKKDGEYETLSVDARPSDALNVAYRCKIPVLVSKQIVYED 240

Query: 241 AIRVSYGFGRVRERKSCYDILLDSAADGPNFLSEELDMLKNMKMAIYEERYKDAAVWRDK 300
           AIRVSYGFGRVRERKSCYDILLDSAADGPNFLSEELDMLKNMKMAIYEERYKDAAVWRDK
Sbjct: 241 AIRVSYGFGRVRERKSCYDILLDSAADGPNFLSEELDMLKNMKMAIYEERYKDAAVWRDK 300

Query: 301 LTKLRKSEHEE 312
           LTKLRKSEHEE
Sbjct: 301 LTKLRKSEHEE 311

[Close]

BLAST of CmaCh06G001370 vs. NCBI nr
Match: XP_022951431.1 (bifunctional nuclease 2 [Cucurbita moschata] >KAG7027810.1 Bifunctional nuclease 2 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 582.4 bits (1500), Expect = 2.3e-162
Identity = 295/311 (94.86%), Postives = 299/311 (96.14%), Query Frame = 0


Query: 1   MLGLTFHFCVPSIFPRSAIAMDHPSTSRPSSSFLHFSLPRSPSIALRSRRSNSILISCHS 60
           MLGLTFHFCVPSIFPRSAIAMDHPST    SS LHFSL RS SIA RSRRSNSILISCHS
Sbjct: 1   MLGLTFHFCVPSIFPRSAIAMDHPST----SSSLHFSLLRSRSIAFRSRRSNSILISCHS 60

Query: 61  SPDGFAHTDDDSHRDYFEASFLVTETINHYRMWKKRFQEDASFNQRESQVNSHSLGLGFF 120
           SPD FAHTDDDS RDYFEASFLVTETINHYRMWKKRFQEDASFNQRES+VNSHSLGLGFF
Sbjct: 61  SPDRFAHTDDDSRRDYFEASFLVTETINHYRMWKKRFQEDASFNQRESRVNSHSLGLGFF 120

Query: 121 RRFRSPTVFLKISCNGDFLLPIVVGEYAIEKLIDCQLGIENGDAPDLFQFIQDLIVKVGY 180
           RRFRSPTVFLKISCNGDFLLPIVVGEYAIEKLIDCQLGIENGDAPDLFQFIQDLIVKVGY
Sbjct: 121 RRFRSPTVFLKISCNGDFLLPIVVGEYAIEKLIDCQLGIENGDAPDLFQFIQDLIVKVGY 180

Query: 181 QVTTARITERVMNTYFARLFLKKDGEYETLSVDARPSDALNVAYRCKIPVLVSKQIVYED 240
           QVTTARITERVMNTYFARLFLKKDGE ETLSVDARPSDALNVAYRCKIPVLVSKQIVYED
Sbjct: 181 QVTTARITERVMNTYFARLFLKKDGENETLSVDARPSDALNVAYRCKIPVLVSKQIVYED 240

Query: 241 AIRVSYGFGRVRERKSCYDILLDSAADGPNFLSEELDMLKNMKMAIYEERYKDAAVWRDK 300
           AIRVSYGFGRVRERKSCYD+LLDSAADGP+FLSEELDMLKNMKMAIYEERYKDAA+WRDK
Sbjct: 241 AIRVSYGFGRVRERKSCYDVLLDSAADGPDFLSEELDMLKNMKMAIYEERYKDAALWRDK 300

Query: 301 LTKLRKSEHEE 312
           LTKLRKSE EE
Sbjct: 301 LTKLRKSEDEE 307

[Close]

BLAST of CmaCh06G001370 vs. NCBI nr
Match: KAG6596262.1 (Bifunctional nuclease 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 536.6 bits (1381), Expect = 1.4e-148
Identity = 271/287 (94.43%), Postives = 276/287 (96.17%), Query Frame = 0


Query: 21  MDHPSTSRPSSSFLHFSLPRSPSIALRSRRSNSILISCHSSPDGFAHTDDDSHRDYFEAS 80
           MDHPST    SS LHFSL RS SIA RSRRSNSILISCHSSPD FAHTDDDS RDYFEAS
Sbjct: 1   MDHPST----SSSLHFSLLRSRSIAFRSRRSNSILISCHSSPDRFAHTDDDSRRDYFEAS 60

Query: 81  FLVTETINHYRMWKKRFQEDASFNQRESQVNSHSLGLGFFRRFRSPTVFLKISCNGDFLL 140
           FLVTETINHYRMWKKRFQEDASFNQRES+VNSHSLGLGFFRRFRSPTVFLKISCNGDFLL
Sbjct: 61  FLVTETINHYRMWKKRFQEDASFNQRESRVNSHSLGLGFFRRFRSPTVFLKISCNGDFLL 120

Query: 141 PIVVGEYAIEKLIDCQLGIENGDAPDLFQFIQDLIVKVGYQVTTARITERVMNTYFARLF 200
           PIVVGEYAIEKLIDCQLGIENGDAPDLFQFIQDLIVKVGYQVTTARITERVMNTYFARLF
Sbjct: 121 PIVVGEYAIEKLIDCQLGIENGDAPDLFQFIQDLIVKVGYQVTTARITERVMNTYFARLF 180

Query: 201 LKKDGEYETLSVDARPSDALNVAYRCKIPVLVSKQIVYEDAIRVSYGFGRVRERKSCYDI 260
           LKKDGE ETLSVDARPSDALNVAYRCKIPVLVSKQIVYEDAIRVSYGFGRVRERKSCYD+
Sbjct: 181 LKKDGENETLSVDARPSDALNVAYRCKIPVLVSKQIVYEDAIRVSYGFGRVRERKSCYDV 240

Query: 261 LLDSAADGPNFLSEELDMLKNMKMAIYEERYKDAAVWRDKLTKLRKS 308
           LLDSAADGP+FLSEELDMLKNMKMAIYEERYKDAA+WRDKLTKLRK+
Sbjct: 241 LLDSAADGPDFLSEELDMLKNMKMAIYEERYKDAALWRDKLTKLRKA 283

[Close]

BLAST of CmaCh06G001370 vs. NCBI nr
Match: XP_038905904.1 (bifunctional nuclease 2 [Benincasa hispida] >XP_038905905.1 bifunctional nuclease 2 [Benincasa hispida] >XP_038905906.1 bifunctional nuclease 2 [Benincasa hispida])

HSP 1 Score: 525.0 bits (1351), Expect = 4.4e-145
Identity = 266/312 (85.26%), Postives = 286/312 (91.67%), Query Frame = 0


Query: 1   MLGLTFHFCVPSIFPRSAIAMDHPSTSRPSSSFLHFSLPRSPSIALRSRRSNSILISCHS 60
           MLGL  HFC P+IFP SAIAMDHPS SRPSSS LHFS+ RS  I +RSRR  SILISCHS
Sbjct: 1   MLGLAPHFCFPTIFPGSAIAMDHPSASRPSSS-LHFSVLRSRPIPVRSRRPKSILISCHS 60

Query: 61  SPDGFAHT-DDDSHRDYFEASFLVTETINHYRMWKKRFQEDASFNQRE-SQVNSHSLGLG 120
           S D   H+ DDDSH+DYFEASFLV+ETI HYRMWKKRFQEDA+FNQRE S+VNSHSLGLG
Sbjct: 61  SRDRSTHSHDDDSHQDYFEASFLVSETIYHYRMWKKRFQEDATFNQRESSRVNSHSLGLG 120

Query: 121 FFRRFRSPTVFLKISCNGDFLLPIVVGEYAIEKLIDCQLGIENGDAPDLFQFIQDLIVKV 180
           FFRRFRSPTVFLKISCNGDFLLPIVVGEYAIEKLIDCQLGIENG+APD+FQFIQDLIVKV
Sbjct: 121 FFRRFRSPTVFLKISCNGDFLLPIVVGEYAIEKLIDCQLGIENGEAPDIFQFIQDLIVKV 180

Query: 181 GYQVTTARITERVMNTYFARLFLKKDGEYETLSVDARPSDALNVAYRCKIPVLVSKQIVY 240
           GY+ TTARITERV+NTYFARLFL+K+GE E LSVDARPSDALN+AYRCKIPVLVSKQIV+
Sbjct: 181 GYEATTARITERVVNTYFARLFLRKEGENEMLSVDARPSDALNIAYRCKIPVLVSKQIVF 240

Query: 241 EDAIRVSYGFGRVRERKSCYDILLDSAADGPNFLSEELDMLKNMKMAIYEERYKDAAVWR 300
           ED IRVSYGFGRV ERKSC+D+LLD AADGP+FLSEELDMLKNMKMAIYEERYKDAA+WR
Sbjct: 241 EDGIRVSYGFGRVHERKSCFDVLLDCAADGPDFLSEELDMLKNMKMAIYEERYKDAAMWR 300

Query: 301 DKLTKLRKSEHE 311
           DKLTKLRKS HE
Sbjct: 301 DKLTKLRKSVHE 311

[Close]

BLAST of CmaCh06G001370 vs. NCBI nr
Match: XP_011650273.1 (bifunctional nuclease 2 [Cucumis sativus] >KGN55629.1 hypothetical protein Csa_011518 [Cucumis sativus])

HSP 1 Score: 518.1 bits (1333), Expect = 5.3e-143
Identity = 262/312 (83.97%), Postives = 284/312 (91.03%), Query Frame = 0


Query: 1   MLGLTFHFCVPSIFPRSAIAMDHPSTSRPSSSFLHFSLPRSPSIALRSRRSNSILISCHS 60
           MLGL  HFC+P+IF  SAIAMDHPS SRPS S LHFSL RS   A+RSRR  SILISCHS
Sbjct: 1   MLGLAPHFCLPTIFFGSAIAMDHPSASRPSCS-LHFSLHRSRPNAVRSRRPKSILISCHS 60

Query: 61  SPDGFAHTDDD--SHRDYFEASFLVTETINHYRMWKKRFQEDASFNQRESQVNSHSLGLG 120
           S D   H+DDD  SH+DYFEASFLV+ETI HYRMWKKRFQEDA+FNQRES+ NS+SLGLG
Sbjct: 61  SRDRSTHSDDDDNSHQDYFEASFLVSETIYHYRMWKKRFQEDANFNQRESRANSNSLGLG 120

Query: 121 FFRRFRSPTVFLKISCNGDFLLPIVVGEYAIEKLIDCQLGIENGDAPDLFQFIQDLIVKV 180
           FFRRFRSPTVFLKISCNGDFLLPIVVGEYAIEKLIDCQLGIENG+APD+FQFIQDLIVKV
Sbjct: 121 FFRRFRSPTVFLKISCNGDFLLPIVVGEYAIEKLIDCQLGIENGEAPDIFQFIQDLIVKV 180

Query: 181 GYQVTTARITERVMNTYFARLFLKKDGEYETLSVDARPSDALNVAYRCKIPVLVSKQIVY 240
           GY+  TARITERV+NTYFARLFL+K+GE E LSVDARPSDALN+AYRCKIPVLVSKQIV+
Sbjct: 181 GYEAITARITERVVNTYFARLFLRKEGESEMLSVDARPSDALNIAYRCKIPVLVSKQIVF 240

Query: 241 EDAIRVSYGFGRVRERKSCYDILLDSAADGPNFLSEELDMLKNMKMAIYEERYKDAAVWR 300
           EDAIRVSYGFGRV ERKSC+D+LLD AADGP+FLSEELDMLKNMK+AIYEERYKDAA+WR
Sbjct: 241 EDAIRVSYGFGRVHERKSCFDVLLDCAADGPDFLSEELDMLKNMKIAIYEERYKDAAMWR 300

Query: 301 DKLTKLRKSEHE 311
           DKLTKLRKS HE
Sbjct: 301 DKLTKLRKSVHE 311

[Close]

BLAST of CmaCh06G001370 vs. TAIR 10
Match: AT5G66050.1 (Wound-responsive family protein )

HSP 1 Score: 235.0 bits (598), Expect = 8.4e-62
Identity = 134/307 (43.65%), Postives = 192/307 (62.54%), Query Frame = 0


Query: 14  FPRSAIAMDHPSTSRPSSSFLHFSLPRSPSIALRSRRSNSILISC--HSSPDGFAHTDDD 73
           F RS  A    S+S PSS       P +   +LRS R   I   C   SS  G    D D
Sbjct: 24  FSRSISASLSSSSSSPSS--FRCCTPLNLK-SLRSHRRPKISHICCDGSSISGSGLGDPD 83

Query: 74  SHRDYFEASFLVTETINHYRMWKKRFQEDA-----------SFNQRESQVNSHSLGLGFF 133
              ++ +AS LV ET  HY+M +  F++D+           S    ES+V    +GLGF 
Sbjct: 84  --LEFLQASVLVAETSMHYKMRRHGFRQDSMWQTSRPLPPFSIRASESRVGVLPIGLGFL 143

Query: 134 RRFRSPTVFLKISCNGDFLLPIVVGEYAIEKLIDCQLGIENGDAPDLFQFIQDLIVKVGY 193
           R+F+ PT+FLKISC+GD+LLP++VG+ A+EKL+D  L     + PD FQF+  ++ K+GY
Sbjct: 144 RQFKQPTIFLKISCDGDYLLPVIVGDAAVEKLLDVPLQGHTEECPDQFQFVSAVVDKLGY 203

Query: 194 QVTTARITERVMNTYFARLFLKKDGEYETLSVDARPSDALNVAYRCKIPVLVSKQIVYED 253
           +V   ++T R++NTY+A L L K G+ E + +D+RPSDA+NVA  C+ P+ V+K IV E+
Sbjct: 204 EVKMVKLTGRIVNTYYASLCLGKPGDIEAICIDSRPSDAINVARACQAPIYVNKAIVLEE 263

Query: 254 AIRVSYGFGRVRERKSCYDILLDSAADGPNFLSEELDMLKNMKMAIYEERYKDAAVWRDK 308
           AI++ YG GR +  K  ++++LDSA DGP+ LSEEL +++NM +A  EERY DAA+WRD+
Sbjct: 264 AIKIGYG-GRPQSAKPVFNVILDSAPDGPDPLSEELKLVRNMDLASKEERYIDAAMWRDR 323

[Close]

BLAST of CmaCh06G001370 vs. TAIR 10
Match: AT5G66050.2 (Wound-responsive family protein )

HSP 1 Score: 222.2 bits (565), Expect = 5.6e-58
Identity = 107/230 (46.52%), Postives = 159/230 (69.13%), Query Frame = 0


Query: 89  HYRMWKKRFQEDA-----------SFNQRESQVNSHSLGLGFFRRFRSPTVFLKISCNGD 148
           HY+M +  F++D+           S    ES+V    +GLGF R+F+ PT+FLKISC+GD
Sbjct: 2   HYKMRRHGFRQDSMWQTSRPLPPFSIRASESRVGVLPIGLGFLRQFKQPTIFLKISCDGD 61

Query: 149 FLLPIVVGEYAIEKLIDCQLGIENGDAPDLFQFIQDLIVKVGYQVTTARITERVMNTYFA 208
           +LLP++VG+ A+EKL+D  L     + PD FQF+  ++ K+GY+V   ++T R++NTY+A
Sbjct: 62  YLLPVIVGDAAVEKLLDVPLQGHTEECPDQFQFVSAVVDKLGYEVKMVKLTGRIVNTYYA 121

Query: 209 RLFLKKDGEYETLSVDARPSDALNVAYRCKIPVLVSKQIVYEDAIRVSYGFGRVRERKSC 268
            L L K G+ E + +D+RPSDA+NVA  C+ P+ V+K IV E+AI++ YG GR +  K  
Sbjct: 122 SLCLGKPGDIEAICIDSRPSDAINVARACQAPIYVNKAIVLEEAIKIGYG-GRPQSAKPV 181

Query: 269 YDILLDSAADGPNFLSEELDMLKNMKMAIYEERYKDAAVWRDKLTKLRKS 308
           ++++LDSA DGP+ LSEEL +++NM +A  EERY DAA+WRD+L  L+ S
Sbjct: 182 FNVILDSAPDGPDPLSEELKLVRNMDLASKEERYIDAAMWRDRLKNLQNS 230

[Close]

BLAST of CmaCh06G001370 vs. TAIR 10
Match: AT1G19660.1 (Wound-responsive family protein )

HSP 1 Score: 132.1 bits (331), Expect = 7.7e-31
Identity = 87/266 (32.71%), Postives = 139/266 (52.26%), Query Frame = 0


Query: 46  LRSRRSNSILISCHSSPDG-FAHTDDDSHRDYFEASFLVTETINHYRMWKKRFQEDASFN 105
           LR  +S   L S HS   G  A   +++  DY ++S L    +   +     F       
Sbjct: 60  LRRYKSIKCLFSSHSDGTGSTAENFNENDEDYVKSSVLEAVEV---KSGPDGFMVKMKDG 119

Query: 106 QRESQVNSHSLGLGFFRRFRSPTVFLKISCNGDFLLPIVVGEYAIEKLIDCQLGIENGDA 165
           ++   V+++  G           + LK+      LLPI+V E     L+     ++    
Sbjct: 120 RQLRCVHNNPQGGNLPNYAPHSAIVLKMEDGTGLLLPIIVLEMPSVLLMAAMTNVQIA-R 179

Query: 166 PDLFQFIQDLIVKVGYQVTTARITERVMNTYFARLFLKKDGE-YETLSVDARPSDALNVA 225
           P ++Q ++D++ K+GY+V   R+T RV   YFA L+L K G+  + +S D RPSDA+N+A
Sbjct: 180 PTMYQVVKDMVDKMGYEVRLVRVTTRVHEAYFAELYLSKVGDKSDCVSFDLRPSDAINIA 239

Query: 226 YRCKIPVLVSKQIVYEDAIRVSYGFGRVRERKSCYDILLDSAADGPN----FLSEELDML 285
            RCK+P+ V+K + Y D +RV    G++ ++    D LL +  D PN    F ++E D++
Sbjct: 240 VRCKVPIQVNKYLAYSDGMRV-IDSGKLSKQTPASDGLLFTELDRPNGQPCFDTKEFDLV 299

Query: 286 KNMKMAIYEERYKDAAVWRDKLTKLR 306
           +NM  A+ EERY +AA WRDKL K +
Sbjct: 300 RNMMQAVDEERYDEAAEWRDKLGKFQ 320

[Close]

BLAST of CmaCh06G001370 vs. TAIR 10
Match: AT1G19660.2 (Wound-responsive family protein )

HSP 1 Score: 132.1 bits (331), Expect = 7.7e-31
Identity = 87/266 (32.71%), Postives = 139/266 (52.26%), Query Frame = 0


Query: 46  LRSRRSNSILISCHSSPDG-FAHTDDDSHRDYFEASFLVTETINHYRMWKKRFQEDASFN 105
           LR  +S   L S HS   G  A   +++  DY ++S L    +   +     F       
Sbjct: 60  LRRYKSIKCLFSSHSDGTGSTAENFNENDEDYVKSSVLEAVEV---KSGPDGFMVKMKDG 119

Query: 106 QRESQVNSHSLGLGFFRRFRSPTVFLKISCNGDFLLPIVVGEYAIEKLIDCQLGIENGDA 165
           ++   V+++  G           + LK+      LLPI+V E     L+     ++    
Sbjct: 120 RQLRCVHNNPQGGNLPNYAPHSAIVLKMEDGTGLLLPIIVLEMPSVLLMAAMTNVQIA-R 179

Query: 166 PDLFQFIQDLIVKVGYQVTTARITERVMNTYFARLFLKKDGE-YETLSVDARPSDALNVA 225
           P ++Q ++D++ K+GY+V   R+T RV   YFA L+L K G+  + +S D RPSDA+N+A
Sbjct: 180 PTMYQVVKDMVDKMGYEVRLVRVTTRVHEAYFAELYLSKVGDKSDCVSFDLRPSDAINIA 239

Query: 226 YRCKIPVLVSKQIVYEDAIRVSYGFGRVRERKSCYDILLDSAADGPN----FLSEELDML 285
            RCK+P+ V+K + Y D +RV    G++ ++    D LL +  D PN    F ++E D++
Sbjct: 240 VRCKVPIQVNKYLAYSDGMRV-IDSGKLSKQTPASDGLLFTELDRPNGQPCFDTKEFDLV 299

Query: 286 KNMKMAIYEERYKDAAVWRDKLTKLR 306
           +NM  A+ EERY +AA WRDKL K +
Sbjct: 300 RNMMQAVDEERYDEAAEWRDKLGKFQ 320

[Close]

BLAST of CmaCh06G001370 vs. TAIR 10
Match: AT1G75380.1 (bifunctional nuclease in basal defense response 1 )

HSP 1 Score: 127.9 bits (320), Expect = 1.4e-29
Identity = 72/185 (38.92%), Postives = 107/185 (57.84%), Query Frame = 0


Query: 126 PTVFLKISCNGDFLLPIVVGEYAIEKLIDCQLGIENGDAPDLFQFIQDLIVKVGYQVTTA 185
           P + LK+      LLPI+V E     L+     ++    P ++Q +++++ K+GY+V   
Sbjct: 134 PAIVLKMEDGTGLLLPIIVLEMPSVLLMAAMTNVQIA-RPTMYQVVKEMVDKMGYEVRLV 193

Query: 186 RITERVMNTYFARLFLKKDGE-YETLSVDARPSDALNVAYRCKIPVLVSKQIVYEDAIRV 245
           R+T+RV   YFA+LFL K G   E +S D RPSDA+N+A RCKIP+ V+K + Y D +RV
Sbjct: 194 RVTKRVHEAYFAQLFLSKVGNASECVSFDLRPSDAINIAVRCKIPIQVNKYLAYSDGMRV 253

Query: 246 SYGFGRVRERKSCYDILLDSAADGPN----FLSEELDMLKNMKMAIYEERYKDAAVWRDK 305
               G++       D LL +  D PN      ++E ++L  M  A+ EERY +AA WRDK
Sbjct: 254 IES-GKISTPAPASDGLLFTEQDRPNGQACLDTKEFNILSKMMQAVDEERYDEAAEWRDK 313

The following BLAST results are available for this feature:

BLAST of CmaCh06G001370 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q93VH2	1.1e-29	32.71	Bifunctional nuclease 2 OS=Arabidopsis thaliana OX=3702 GN=BBD2 PE=2 SV=1	[more]
Q9FWS6	2.0e-28	38.92	Bifunctional nuclease 1 OS=Arabidopsis thaliana OX=3702 GN=BBD1 PE=2 SV=1	[more]
Q6YZM6	4.5e-28	36.82	Bifunctional nuclease 2 OS=Oryza sativa subsp. japonica OX=39947 GN=BBD2 PE=2 SV...	[more]
Q5N8J3	1.5e-26	37.91	Bifunctional nuclease 1 OS=Oryza sativa subsp. japonica OX=39947 GN=BBD1 PE=2 SV...	[more]
Q09LL3	1.5e-26	37.91	Bifunctional nuclease OS=Oryza minuta OX=63629 GN=BBD PE=2 SV=1	[more]

BLAST of CmaCh06G001370 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1I8T8	1.1e-175	100.00	bifunctional nuclease 2-like OS=Cucurbita maxima OX=3661 GN=LOC111470219 PE=3 SV...	[more]
A0A6J1GHK4	1.1e-162	94.86	bifunctional nuclease 2 OS=Cucurbita moschata OX=3662 GN=LOC111454246 PE=3 SV=1	[more]
A0A0A0L3L8	2.6e-143	83.97	BFN domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G002490 PE=3 SV...	[more]
A0A6J1HJX9	5.8e-143	83.87	bifunctional nuclease 2-like OS=Cucurbita maxima OX=3661 GN=LOC111465202 PE=3 SV...	[more]
A0A6J1E735	3.7e-142	83.55	bifunctional nuclease 2-like OS=Cucurbita moschata OX=3662 GN=LOC111431208 PE=3 ...	[more]

BLAST of CmaCh06G001370 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022971524.1	2.4e-175	100.00	bifunctional nuclease 2-like [Cucurbita maxima]	[more]
XP_022951431.1	2.3e-162	94.86	bifunctional nuclease 2 [Cucurbita moschata] >KAG7027810.1 Bifunctional nuclease...	[more]
KAG6596262.1	1.4e-148	94.43	Bifunctional nuclease 2, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_038905904.1	4.4e-145	85.26	bifunctional nuclease 2 [Benincasa hispida] >XP_038905905.1 bifunctional nucleas...	[more]
XP_011650273.1	5.3e-143	83.97	bifunctional nuclease 2 [Cucumis sativus] >KGN55629.1 hypothetical protein Csa_0...	[more]

BLAST of CmaCh06G001370 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G66050.1	8.4e-62	43.65	Wound-responsive family protein	[more]
AT5G66050.2	5.6e-58	46.52	Wound-responsive family protein	[more]
AT1G19660.1	7.7e-31	32.71	Wound-responsive family protein	[more]
AT1G19660.2	7.7e-31	32.71	Wound-responsive family protein	[more]
AT1G75380.1	1.4e-29	38.92	bifunctional nuclease in basal defense response 1	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR036104	Bifunctional nuclease superfamily	GENE3D	3.10.690.10	Bifunctional nuclease domain	coord: 99..247 e-value: 1.8E-27 score: 97.6
IPR036104	Bifunctional nuclease superfamily	SUPERFAMILY	103256	Hypothetical protein TM0160	coord: 115..245
IPR003729	Bifunctional nuclease domain	PFAM	PF02577	DUF151	coord: 137..244 e-value: 1.5E-16 score: 60.1
IPR003729	Bifunctional nuclease domain	PROSITE	PS51658	BFN	coord: 107..244 score: 26.074026
None	No IPR available	PANTHER	PTHR15160:SF1	VON HIPPEL-LINDAU DISEASE TUMOR SUPPRESSOR-RELATED	coord: 47..304
None	No IPR available	PANTHER	PTHR15160	VON HIPPEL-LINDAU PROTEIN	coord: 47..304

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmaCh06G001370.1	CmaCh06G001370.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0090305	nucleic acid phosphodiester bond hydrolysis
biological_process	GO:0016567	protein ubiquitination
cellular_component	GO:0005634	nucleus
cellular_component	GO:0030891	VCB complex
molecular_function	GO:0004518	nuclease activity