CmaCh05G014140 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh05G014140
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
DescriptionThreonine synthase
LocationCma_Chr05: 10522927 .. 10524531 (+)
RNA-Seq ExpressionCmaCh05G014140
SyntenyCmaCh05G014140
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGACTCCTCCCTCTTCTCTTTCAATACCTCTCTCTCTCCCAAACCCTCTTCTCTCTTCCGACGCCCCCTTCGCCGGAATTCTTCCCCCCCTTTCACCATCGCCTGCCCCTCCGCCACCTCCGACTCTACCGCCTCCCCTGTTCTCGACTCTTCCTTGCCGCTCGGAGTTCAGAATCCGCAGTTCGCTTGTGTCGATATCCAAGATGACTCTCATCGCAGTCGTTCCGTTGCTGGCGACGGCGGCCGTAATTTCTCTGCGAAGTATGTGCCGTTTGGTTCTGGCTTTGATTCATCCGAATGGTACTCGCTCGACGAAATCGTTTATCGGAGCCAATCCGGCGGGCTGCTCGACGTTCAGCACAACATGGAAGCGTTGAAGGAGTTCGATGGAGCGTATTGGCGGAATCTGTTCGATTCGCGTGTAGGGAAGACGACCTGGCCGTACGGTTCCGGCGTGTGGAGCAAGAAAGAGTGGGTGCTGCCGGAGATTGACTCCAGCGACATCGTTAGCGCCTTCGAAGGAAATTCGAATCTCTTCTGGGCGGAGCGTTTTGGGAAGGAGTTTCTAGGCATGAACGATCTGTGGGTCAAACATTGCGGGATTAGCCACACCGGCAGTTTTAAAGACCTTGGAATGACTGTTTTGGTCAGCCAAGTCAACCGCCTCCGGAAAATGAATCGCCCGGTCGTCGGTGTCGGCTGCGCCTCCACTGGAGACACCTCCGCCGCTCTCTCTGCTTATTGCGCCGCCGCAGGAATTCCGTCCATTGTCTTCCTCCCGGCCGATAAGATTTCCATGGCACAATTAGTTCAGCCGATTGCAAACGGCGCTTTCGTCTTAAGCATGGACACCGATTTCGACGGATGTATGAAATTAATCAGGGAAATCACGGCGGAGCTTCCGATTTATCTGGCGAATTCGCTCAATAGTTTGAGACTGGAAGGGCAAAAGACGGCGGCCATTGAAATCCTTCAACAGTTCGATTGGGAAGTGCCAGATTGGGTAATCATACCAGGGGGAAACCTAGGGAATATATATGCGTTTTACAAAGGCTTCCAAATGTGCAAAGAATTAGGTCTTGTGGACAAGATTCCACGGCTGGTTTGTGCTCAAGCGGCAAATGCAAATCCGCTTTACTTGTATTACAAATCTGGCTGGAAAGAGTTCTCGCCATTGAAGGCGAGCACCACTTTCGCTTCTGCAATACAGATTGGGGATCCGGTTTCCATTGACAGAGCCGTGTTTGCTTTGAAAAATTCCGGCGGAATCGTGGAAGAAGCGACGGAGGAGGAACTAATGGACGCAACAGCTCAGGCTGATTCGACGGGAATGTTCATTTGCCCTCACACGGGAGTGGCGTTAACGGCTCTGATTAAGCTGAGAAACAGGGGCGTGATCAAAGGCAGTGATCGGACGGTGGTAGTAAGCACTGCTCATGGTCTGAAATTCACTCAATCCAAGATCGATTACCATTCGAGAGCAATCCAGGGCATGGAGTGCCGATTTGCAAACCCGCCGGTGGAAGTGAAGGCGGATTTTGGGGCTGTAATGGATGTTCTTAAGGAGCATCTGTCGAGGAAGAATCCAAAAATGGAGCTATAG

mRNA sequence

ATGGACTCCTCCCTCTTCTCTTTCAATACCTCTCTCTCTCCCAAACCCTCTTCTCTCTTCCGACGCCCCCTTCGCCGGAATTCTTCCCCCCCTTTCACCATCGCCTGCCCCTCCGCCACCTCCGACTCTACCGCCTCCCCTGTTCTCGACTCTTCCTTGCCGCTCGGAGTTCAGAATCCGCAGTTCGCTTGTGTCGATATCCAAGATGACTCTCATCGCAGTCGTTCCGTTGCTGGCGACGGCGGCCGTAATTTCTCTGCGAAGTATGTGCCGTTTGGTTCTGGCTTTGATTCATCCGAATGGTACTCGCTCGACGAAATCGTTTATCGGAGCCAATCCGGCGGGCTGCTCGACGTTCAGCACAACATGGAAGCGTTGAAGGAGTTCGATGGAGCGTATTGGCGGAATCTGTTCGATTCGCGTGTAGGGAAGACGACCTGGCCGTACGGTTCCGGCGTGTGGAGCAAGAAAGAGTGGGTGCTGCCGGAGATTGACTCCAGCGACATCGTTAGCGCCTTCGAAGGAAATTCGAATCTCTTCTGGGCGGAGCGTTTTGGGAAGGAGTTTCTAGGCATGAACGATCTGTGGGTCAAACATTGCGGGATTAGCCACACCGGCAGTTTTAAAGACCTTGGAATGACTGTTTTGGTCAGCCAAGTCAACCGCCTCCGGAAAATGAATCGCCCGGTCGTCGGTGTCGGCTGCGCCTCCACTGGAGACACCTCCGCCGCTCTCTCTGCTTATTGCGCCGCCGCAGGAATTCCGTCCATTGTCTTCCTCCCGGCCGATAAGATTTCCATGGCACAATTAGTTCAGCCGATTGCAAACGGCGCTTTCGTCTTAAGCATGGACACCGATTTCGACGGATGTATGAAATTAATCAGGGAAATCACGGCGGAGCTTCCGATTTATCTGGCGAATTCGCTCAATAGTTTGAGACTGGAAGGGCAAAAGACGGCGGCCATTGAAATCCTTCAACAGTTCGATTGGGAAGTGCCAGATTGGGTAATCATACCAGGGGGAAACCTAGGGAATATATATGCGTTTTACAAAGGCTTCCAAATGTGCAAAGAATTAGGTCTTGTGGACAAGATTCCACGGCTGGTTTGTGCTCAAGCGGCAAATGCAAATCCGCTTTACTTGTATTACAAATCTGGCTGGAAAGAGTTCTCGCCATTGAAGGCGAGCACCACTTTCGCTTCTGCAATACAGATTGGGGATCCGGTTTCCATTGACAGAGCCGTGTTTGCTTTGAAAAATTCCGGCGGAATCGTGGAAGAAGCGACGGAGGAGGAACTAATGGACGCAACAGCTCAGGCTGATTCGACGGGAATGTTCATTTGCCCTCACACGGGAGTGGCGTTAACGGCTCTGATTAAGCTGAGAAACAGGGGCGTGATCAAAGGCAGTGATCGGACGGTGGTAGTAAGCACTGCTCATGGTCTGAAATTCACTCAATCCAAGATCGATTACCATTCGAGAGCAATCCAGGGCATGGAGTGCCGATTTGCAAACCCGCCGGTGGAAGTGAAGGCGGATTTTGGGGCTGTAATGGATGTTCTTAAGGAGCATCTGTCGAGGAAGAATCCAAAAATGGAGCTATAG

Coding sequence (CDS)

ATGGACTCCTCCCTCTTCTCTTTCAATACCTCTCTCTCTCCCAAACCCTCTTCTCTCTTCCGACGCCCCCTTCGCCGGAATTCTTCCCCCCCTTTCACCATCGCCTGCCCCTCCGCCACCTCCGACTCTACCGCCTCCCCTGTTCTCGACTCTTCCTTGCCGCTCGGAGTTCAGAATCCGCAGTTCGCTTGTGTCGATATCCAAGATGACTCTCATCGCAGTCGTTCCGTTGCTGGCGACGGCGGCCGTAATTTCTCTGCGAAGTATGTGCCGTTTGGTTCTGGCTTTGATTCATCCGAATGGTACTCGCTCGACGAAATCGTTTATCGGAGCCAATCCGGCGGGCTGCTCGACGTTCAGCACAACATGGAAGCGTTGAAGGAGTTCGATGGAGCGTATTGGCGGAATCTGTTCGATTCGCGTGTAGGGAAGACGACCTGGCCGTACGGTTCCGGCGTGTGGAGCAAGAAAGAGTGGGTGCTGCCGGAGATTGACTCCAGCGACATCGTTAGCGCCTTCGAAGGAAATTCGAATCTCTTCTGGGCGGAGCGTTTTGGGAAGGAGTTTCTAGGCATGAACGATCTGTGGGTCAAACATTGCGGGATTAGCCACACCGGCAGTTTTAAAGACCTTGGAATGACTGTTTTGGTCAGCCAAGTCAACCGCCTCCGGAAAATGAATCGCCCGGTCGTCGGTGTCGGCTGCGCCTCCACTGGAGACACCTCCGCCGCTCTCTCTGCTTATTGCGCCGCCGCAGGAATTCCGTCCATTGTCTTCCTCCCGGCCGATAAGATTTCCATGGCACAATTAGTTCAGCCGATTGCAAACGGCGCTTTCGTCTTAAGCATGGACACCGATTTCGACGGATGTATGAAATTAATCAGGGAAATCACGGCGGAGCTTCCGATTTATCTGGCGAATTCGCTCAATAGTTTGAGACTGGAAGGGCAAAAGACGGCGGCCATTGAAATCCTTCAACAGTTCGATTGGGAAGTGCCAGATTGGGTAATCATACCAGGGGGAAACCTAGGGAATATATATGCGTTTTACAAAGGCTTCCAAATGTGCAAAGAATTAGGTCTTGTGGACAAGATTCCACGGCTGGTTTGTGCTCAAGCGGCAAATGCAAATCCGCTTTACTTGTATTACAAATCTGGCTGGAAAGAGTTCTCGCCATTGAAGGCGAGCACCACTTTCGCTTCTGCAATACAGATTGGGGATCCGGTTTCCATTGACAGAGCCGTGTTTGCTTTGAAAAATTCCGGCGGAATCGTGGAAGAAGCGACGGAGGAGGAACTAATGGACGCAACAGCTCAGGCTGATTCGACGGGAATGTTCATTTGCCCTCACACGGGAGTGGCGTTAACGGCTCTGATTAAGCTGAGAAACAGGGGCGTGATCAAAGGCAGTGATCGGACGGTGGTAGTAAGCACTGCTCATGGTCTGAAATTCACTCAATCCAAGATCGATTACCATTCGAGAGCAATCCAGGGCATGGAGTGCCGATTTGCAAACCCGCCGGTGGAAGTGAAGGCGGATTTTGGGGCTGTAATGGATGTTCTTAAGGAGCATCTGTCGAGGAAGAATCCAAAAATGGAGCTATAG

Protein sequence

MDSSLFSFNTSLSPKPSSLFRRPLRRNSSPPFTIACPSATSDSTASPVLDSSLPLGVQNPQFACVDIQDDSHRSRSVAGDGGRNFSAKYVPFGSGFDSSEWYSLDEIVYRSQSGGLLDVQHNMEALKEFDGAYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDSSDIVSAFEGNSNLFWAERFGKEFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKMNRPVVGVGCASTGDTSAALSAYCAAAGIPSIVFLPADKISMAQLVQPIANGAFVLSMDTDFDGCMKLIREITAELPIYLANSLNSLRLEGQKTAAIEILQQFDWEVPDWVIIPGGNLGNIYAFYKGFQMCKELGLVDKIPRLVCAQAANANPLYLYYKSGWKEFSPLKASTTFASAIQIGDPVSIDRAVFALKNSGGIVEEATEEELMDATAQADSTGMFICPHTGVALTALIKLRNRGVIKGSDRTVVVSTAHGLKFTQSKIDYHSRAIQGMECRFANPPVEVKADFGAVMDVLKEHLSRKNPKMEL
Homology
BLAST of CmaCh05G014140 vs. ExPASy Swiss-Prot
Match: Q9MT28 (Threonine synthase, chloroplastic OS=Solanum tuberosum OX=4113 PE=2 SV=1)

HSP 1 Score: 827.0 bits (2135), Expect = 1.2e-238
Identity = 399/464 (85.99%), Postives = 433/464 (93.32%), Query Frame = 0

Query: 69  DDSHRSRSVAGDGGRNFSAKYVPFGSGFDSSEWYSLDEIVYRSQSGGLLDVQHNMEALKE 128
           D++ R  +       NFSA+YVPF +G +S EWYSLDEIVYRS+SGGLLDVQH+M+ALK+
Sbjct: 56  DENIREEARRHCSSHNFSARYVPFNAGPNSDEWYSLDEIVYRSRSGGLLDVQHDMDALKK 115

Query: 129 FDGAYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDSSDIVSAFEGNSNLFWAERFGKE 188
           FDG YWR+LFDSRVGKTTWPYGSGVWSKKEWVLPEIDS DIVSAFEGNSNLFWAERFGK+
Sbjct: 116 FDGQYWRSLFDSRVGKTTWPYGSGVWSKKEWVLPEIDSDDIVSAFEGNSNLFWAERFGKQ 175

Query: 189 FLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKMNRPVVGVGCASTGDTSAALSAY 248
           FLGM DLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKM++PVVGVGCASTGDTSAALSAY
Sbjct: 176 FLGMTDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKMHKPVVGVGCASTGDTSAALSAY 235

Query: 249 CAAAGIPSIVFLPADKISMAQLVQPIANGAFVLSMDTDFDGCMKLIREITAELPIYLANS 308
           CA+AGIPSIVFLPA+KISMAQLVQPIANGAFVLS+DTDFDGCM+LIRE+TAELPIYLANS
Sbjct: 236 CASAGIPSIVFLPANKISMAQLVQPIANGAFVLSIDTDFDGCMQLIREVTAELPIYLANS 295

Query: 309 LNSLRLEGQKTAAIEILQQFDWEVPDWVIIPGGNLGNIYAFYKGFQMCKELGLVDKIPRL 368
           LNSLRLEGQKTAAIEILQQFDWEVP+WVI+PGGNLGNIYAFYKGFQMCKELGLVD+IPRL
Sbjct: 296 LNSLRLEGQKTAAIEILQQFDWEVPEWVIVPGGNLGNIYAFYKGFQMCKELGLVDRIPRL 355

Query: 369 VCAQAANANPLYLYYKSGWKEFSPLKASTTFASAIQIGDPVSIDRAVFALKNSGGIVEEA 428
           VCAQAANANPLYL+YKSGWK+F P+KA+TTFASAIQIGDPVSIDRAVFAL+   GIVEEA
Sbjct: 356 VCAQAANANPLYLHYKSGWKDFKPVKANTTFASAIQIGDPVSIDRAVFALQQCNGIVEEA 415

Query: 429 TEEELMDATAQADSTGMFICPHTGVALTALIKLRNRGVIKGSDRTVVVSTAHGLKFTQSK 488
           TEEELMDA AQADSTGMFICPHTGVALTAL KLRN GVI  +DRTVVVSTAHGLKFTQSK
Sbjct: 416 TEEELMDAMAQADSTGMFICPHTGVALTALFKLRNSGVIAPTDRTVVVSTAHGLKFTQSK 475

Query: 489 IDYHSRAIQGMECRFANPPVEVKADFGAVMDVLKEHLSRKNPKM 533
           IDYHS+ I+ MECRFANPPVEVKADFG+VMDVLK +L  +N K+
Sbjct: 476 IDYHSKEIKDMECRFANPPVEVKADFGSVMDVLKSYLLSQNSKL 519

BLAST of CmaCh05G014140 vs. ExPASy Swiss-Prot
Match: Q9S7B5 (Threonine synthase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=TS1 PE=1 SV=1)

HSP 1 Score: 818.5 bits (2113), Expect = 4.3e-236
Identity = 413/524 (78.82%), Postives = 457/524 (87.21%), Query Frame = 0

Query: 3   SSLFSFN-TSLSPKPSSLFRRPLRRNSSPPFTIACPSATSDSTASPVLDSSLPLGVQNPQ 62
           S LF+ + +SL+PK     + P+RR+ S       P   S +     + + +   V+ P 
Sbjct: 4   SCLFNASVSSLNPK-----QDPIRRHRSTSLLRHRPVVISCTADGNNIKAPIETAVKPPH 63

Query: 63  FACVDIQDDSHRSRSVAGDGGRNFSAKYVPFGSGFDSSEWYSLDEIVYRSQSGGLLDVQH 122
               +I+D++ R+RS A +    FSAKYVPF +   S+E YSLDEIVYRS+SGGLLDV+H
Sbjct: 64  RTEDNIRDEARRNRSNAVN---PFSAKYVPFNAAPGSTESYSLDEIVYRSRSGGLLDVEH 123

Query: 123 NMEALKEFDGAYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDSSDIVSAFEGNSNLFW 182
           +MEALK FDGAYWR+LFDSRVGK+TWPYGSGVWSKKEWVLPEID  DIVSAFEGNSNLFW
Sbjct: 124 DMEALKRFDGAYWRDLFDSRVGKSTWPYGSGVWSKKEWVLPEIDDDDIVSAFEGNSNLFW 183

Query: 183 AERFGKEFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKMNRPVVGVGCASTGDT 242
           AERFGK+FLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKM RPVVGVGCASTGDT
Sbjct: 184 AERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKMKRPVVGVGCASTGDT 243

Query: 243 SAALSAYCAAAGIPSIVFLPADKISMAQLVQPIANGAFVLSMDTDFDGCMKLIREITAEL 302
           SAALSAYCA+AGIPSIVFLPA+KISMAQLVQPIANGAFVLS+DTDFDGCMKLIREITAEL
Sbjct: 244 SAALSAYCASAGIPSIVFLPANKISMAQLVQPIANGAFVLSIDTDFDGCMKLIREITAEL 303

Query: 303 PIYLANSLNSLRLEGQKTAAIEILQQFDWEVPDWVIIPGGNLGNIYAFYKGFQMCKELGL 362
           PIYLANSLNSLRLEGQKTAAIEILQQFDW+VPDWVI+PGGNLGNIYAFYKGF+MC+ELGL
Sbjct: 304 PIYLANSLNSLRLEGQKTAAIEILQQFDWQVPDWVIVPGGNLGNIYAFYKGFKMCQELGL 363

Query: 363 VDKIPRLVCAQAANANPLYLYYKSGWKEFSPLKASTTFASAIQIGDPVSIDRAVFALKNS 422
           VD+IPR+VCAQAANANPLYL+YKSGWK+F P+ ASTTFASAIQIGDPVSIDRAV+ALK  
Sbjct: 364 VDRIPRMVCAQAANANPLYLHYKSGWKDFKPMTASTTFASAIQIGDPVSIDRAVYALKKC 423

Query: 423 GGIVEEATEEELMDATAQADSTGMFICPHTGVALTALIKLRNRGVIKGSDRTVVVSTAHG 482
            GIVEEATEEELMDA AQADSTGMFICPHTGVALTAL KLRN+GVI  +DRTVVVSTAHG
Sbjct: 424 NGIVEEATEEELMDAMAQADSTGMFICPHTGVALTALFKLRNQGVIAPTDRTVVVSTAHG 483

Query: 483 LKFTQSKIDYHSRAIQGMECRFANPPVEVKADFGAVMDVLKEHL 526
           LKFTQSKIDYHS AI  M CRF+NPPV+VKADFGAVMDVLK +L
Sbjct: 484 LKFTQSKIDYHSNAIPDMACRFSNPPVDVKADFGAVMDVLKSYL 519

BLAST of CmaCh05G014140 vs. ExPASy Swiss-Prot
Match: Q9SSP5 (Threonine synthase 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=TS2 PE=1 SV=1)

HSP 1 Score: 790.0 bits (2039), Expect = 1.6e-227
Identity = 398/504 (78.97%), Postives = 440/504 (87.30%), Query Frame = 0

Query: 24  LRRNSSPPFTIACPSATSDSTASPVLDSSLPLGVQNPQFA-CVDIQDDSHRSRSVAGDGG 83
           L+ +S+  FT+ C S      ASP +    P   Q P+ +   +I+D++ R         
Sbjct: 21  LKPHSAASFTVRCTS------ASPAVPPQTP---QKPRRSPDENIRDEARRRPHQL---- 80

Query: 84  RNFSAKYVPFGSGFDSSEWYSLDEIVYRSQSGGLLDVQHNMEALKEFDGAYWRNLFDSRV 143
           +N SA+YVPF +   S+E YSLDEIVYRSQSG LLDVQH+  ALK +DG +WRNLFDSRV
Sbjct: 81  QNLSARYVPFNAPPSSTESYSLDEIVYRSQSGALLDVQHDFAALKRYDGEFWRNLFDSRV 140

Query: 144 GKTTWPYGSGVWSKKEWVLPEIDSSDIVSAFEGNSNLFWAERFGKEFLGMNDLWVKHCGI 203
           GKT WPYGSGVWSKKEWVLPEID  DIVSAFEGNSNLFWAERFGK++L MNDLWVKHCGI
Sbjct: 141 GKTNWPYGSGVWSKKEWVLPEIDDDDIVSAFEGNSNLFWAERFGKQYLQMNDLWVKHCGI 200

Query: 204 SHTGSFKDLGMTVLVSQVNRLRKMNRPVVGVGCASTGDTSAALSAYCAAAGIPSIVFLPA 263
           SHTGSFKDLGM+VLVSQVNRLRKMN+PV+GVGCASTGDTSAALSAYCA+AGIPSIVFLPA
Sbjct: 201 SHTGSFKDLGMSVLVSQVNRLRKMNKPVIGVGCASTGDTSAALSAYCASAGIPSIVFLPA 260

Query: 264 DKISMAQLVQPIANGAFVLSMDTDFDGCMKLIREITAELPIYLANSLNSLRLEGQKTAAI 323
           DKISMAQLVQPIANGAFVLS+DTDFDGCM LIRE+TAELPIYLANSLNSLRLEGQKTAAI
Sbjct: 261 DKISMAQLVQPIANGAFVLSIDTDFDGCMHLIREVTAELPIYLANSLNSLRLEGQKTAAI 320

Query: 324 EILQQFDWEVPDWVIIPGGNLGNIYAFYKGFQMCKELGLVDKIPRLVCAQAANANPLYLY 383
           EILQQF+W+VPDWVI+PGGNLGNIYAFYKGF MCKELGLVD+IPRLVCAQAANANPLYL+
Sbjct: 321 EILQQFNWQVPDWVIVPGGNLGNIYAFYKGFHMCKELGLVDRIPRLVCAQAANANPLYLH 380

Query: 384 YKSGWKE-FSPLKASTTFASAIQIGDPVSIDRAVFALKNSGGIVEEATEEELMDATAQAD 443
           YKSG+KE F+PLKA+TTFASAIQIGDPVSIDRAV+ALK S GIVEEATEEELMDATA AD
Sbjct: 381 YKSGFKEDFNPLKANTTFASAIQIGDPVSIDRAVYALKKSNGIVEEATEEELMDATALAD 440

Query: 444 STGMFICPHTGVALTALIKLRNRGVIKGSDRTVVVSTAHGLKFTQSKIDYHSRAIQGMEC 503
           STGMFICPHTGVALTAL+KLR  GVI  +DRTVVVSTAHGLKFTQSKIDYHS+ I+ M C
Sbjct: 441 STGMFICPHTGVALTALMKLRKSGVIGANDRTVVVSTAHGLKFTQSKIDYHSKNIKEMAC 500

Query: 504 RFANPPVEVKADFGAVMDVLKEHL 526
           R ANPPV+VKA FG+VMDVLKE+L
Sbjct: 501 RLANPPVKVKAKFGSVMDVLKEYL 511

BLAST of CmaCh05G014140 vs. ExPASy Swiss-Prot
Match: Q58860 (Threonine synthase OS=Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) OX=243232 GN=thrC PE=3 SV=1)

HSP 1 Score: 278.5 bits (711), Expect = 1.6e-73
Identity = 160/423 (37.83%), Postives = 247/423 (58.39%), Query Frame = 0

Query: 102 YSLDEIVYRSQSGGLLDVQHNMEALKEFDGAYWRNLFDSRVGKTTWPYGSGVWSKKEWVL 161
           Y +DEI+Y  + GGLL++ ++ E +K+        + + ++ K       GVW   E+ L
Sbjct: 12  YDVDEIIYTCECGGLLEIIYDYEEIKD-------KVSEEKLRKRE----IGVWRYLEY-L 71

Query: 162 PEIDSSDIVSAFEGNSNLFWAERFGKEFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVN 221
           P  D S IVS  EG + L+      KE LG+ +L+VK+ G + TGSFKD GMTV V++ N
Sbjct: 72  PVKDESKIVSLCEGGTPLYRCNNLEKE-LGIKELYVKNEGANPTGSFKDRGMTVGVTRAN 131

Query: 222 RLRKMNRPVVGVGCASTGDTSAALSAYCAAAGIPSIVFLPADKISMAQLVQPIANGAFVL 281
            L      V  VGCASTG+TSA+L+AY A +G   IV LP  K+++ +L Q +  GA V+
Sbjct: 132 EL-----GVEVVGCASTGNTSASLAAYSARSGKKCIVLLPEGKVALGKLAQAMFYGAKVI 191

Query: 282 SMDTDFDGCMKLIREITAELPIYLANSLNSLRLEGQKTAAIEILQQFDWEVPDWVIIPGG 341
            +  +FD  + +++++  E  IYL NS+N  RLEGQKT A EI  Q +W+VPD VI+P G
Sbjct: 192 QVKGNFDDALDMVKQLAKEKLIYLLNSINPFRLEGQKTIAFEICDQLNWQVPDRVIVPVG 251

Query: 342 NLGNIYAFYKGFQMCKELGLVDKIPRLVCAQAANANPLYLYYKSGWKEFSPLKASTTFAS 401
           N GNI A +KGF+  +  G++D++P++   QA  A P+   ++   K+  P K   T A+
Sbjct: 252 NAGNISAIWKGFKEFEITGIIDELPKMTGIQADGAKPIVEAFRKRAKDIIPYKNPETIAT 311

Query: 402 AIQIGDPVSIDRAVFALKNSGGIVEEATEEELMDA-TAQADSTGMFICPHTGVALTALIK 461
           AI+IG+PV+  +A+ A+ +SGG  E  T+EE+++A    A   G+F+ P +  ++  L K
Sbjct: 312 AIRIGNPVNAPKALDAIYSSGGYAEAVTDEEIVEAQKLLARKEGIFVEPASASSIAGLKK 371

Query: 462 LRNRGVIKGSDRTVVVSTAHGLKFTQSKIDYHSRAIQGMECRFANPPVEVKADFGAVMDV 521
           L   G+I   +R V ++T HGLK   + I            R +  P++++ D   +  +
Sbjct: 372 LLEEGIIDRDERIVCITTGHGLKDPDAAI------------RASEEPIKIECDMNVLKRI 404

Query: 522 LKE 524
           LKE
Sbjct: 432 LKE 404

BLAST of CmaCh05G014140 vs. ExPASy Swiss-Prot
Match: Q8TQD4 (Threonine synthase OS=Methanosarcina acetivorans (strain ATCC 35395 / DSM 2834 / JCM 12185 / C2A) OX=188937 GN=thrC PE=1 SV=1)

HSP 1 Score: 251.1 bits (640), Expect = 2.7e-65
Identity = 157/391 (40.15%), Postives = 230/391 (58.82%), Query Frame = 0

Query: 102 YSLDEIVYR-SQSGGLLDVQHNMEALKEFDGAYWRNLFDSRVGKTTWPYGSGVWSKKEWV 161
           YS DE++Y  S+  GLLDV ++  ++K           D    KT  P    VW K   +
Sbjct: 13  YSRDEVIYTCSKCDGLLDVIYDYSSIK----------IDMEKLKTECP---SVW-KYAKL 72

Query: 162 LPEIDSSDIVSAFEGNSNLFWAERFGKEFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQV 221
           LP     + V+  EG + L+  +R   E +G+  L+VKH G++ TGSFKD GMTV V++ 
Sbjct: 73  LPV--EREPVTIQEGGTPLYKCDRLA-EKIGIKKLYVKHEGMNPTGSFKDRGMTVGVTKA 132

Query: 222 NRLRKMNRPVVGVGCASTGDTSAALSAYCAAAGIPSIVFLPADKISMAQLVQPIANGAFV 281
             L  MN     V CASTG+TSAAL+ Y A AGIP +V LPA K+++ ++ Q + +GA V
Sbjct: 133 LEL-GMNT----VACASTGNTSAALAIYGAKAGIPVVVLLPAGKVALGKVAQALMHGAKV 192

Query: 282 LSMDTDFDGCMKLIREITAELPIYLANSLNSLRLEGQKTAAIEILQQFDWEVPDWVIIPG 341
           LS+  +FD  + L+R + ++  IYL NS+N  RLEGQKT   EI  Q D++VPD +++P 
Sbjct: 193 LSIRGNFDDALALVRTLCSQEKIYLLNSINPYRLEGQKTIGFEIADQLDFKVPDRIVLPV 252

Query: 342 GNLGNIYAFYKGFQMCKELGLVDKIPRLVCAQAANANPLYLYYKSGWKEFSPLKASTTFA 401
           GN GNI A YKGF+  K LG+ D +P++   QA  + P+    KSG    +P +   T A
Sbjct: 253 GNAGNITAIYKGFREFKILGITDSLPKMTGIQAEGSCPIVKAIKSGAPAITPEENPETVA 312

Query: 402 SAIQIGDPVSIDRAVFALKNSGGIVEEATEEELMDATAQ-ADSTGMFICPHTGVALTALI 461
           +AI+IG+PV+  +A+ A++ SGG  E  T+EE++ A    A   G+ + P +  ++  L 
Sbjct: 313 TAIRIGNPVNATKALSAIRESGGTAESVTDEEILAAQKDLARLEGIGVEPASAASVAGLR 372

Query: 462 KLRNRGVIKGSDRTVVVSTAHGLKFTQSKID 491
           KL + GVI   +  V ++T H LK  Q+ ID
Sbjct: 373 KLVDMGVIGRDETVVCITTGHLLKDPQTVID 381

BLAST of CmaCh05G014140 vs. TAIR 10
Match: AT4G29840.1 (Pyridoxal-5'-phosphate-dependent enzyme family protein )

HSP 1 Score: 818.5 bits (2113), Expect = 3.1e-237
Identity = 413/524 (78.82%), Postives = 457/524 (87.21%), Query Frame = 0

Query: 3   SSLFSFN-TSLSPKPSSLFRRPLRRNSSPPFTIACPSATSDSTASPVLDSSLPLGVQNPQ 62
           S LF+ + +SL+PK     + P+RR+ S       P   S +     + + +   V+ P 
Sbjct: 4   SCLFNASVSSLNPK-----QDPIRRHRSTSLLRHRPVVISCTADGNNIKAPIETAVKPPH 63

Query: 63  FACVDIQDDSHRSRSVAGDGGRNFSAKYVPFGSGFDSSEWYSLDEIVYRSQSGGLLDVQH 122
               +I+D++ R+RS A +    FSAKYVPF +   S+E YSLDEIVYRS+SGGLLDV+H
Sbjct: 64  RTEDNIRDEARRNRSNAVN---PFSAKYVPFNAAPGSTESYSLDEIVYRSRSGGLLDVEH 123

Query: 123 NMEALKEFDGAYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDSSDIVSAFEGNSNLFW 182
           +MEALK FDGAYWR+LFDSRVGK+TWPYGSGVWSKKEWVLPEID  DIVSAFEGNSNLFW
Sbjct: 124 DMEALKRFDGAYWRDLFDSRVGKSTWPYGSGVWSKKEWVLPEIDDDDIVSAFEGNSNLFW 183

Query: 183 AERFGKEFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKMNRPVVGVGCASTGDT 242
           AERFGK+FLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKM RPVVGVGCASTGDT
Sbjct: 184 AERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKMKRPVVGVGCASTGDT 243

Query: 243 SAALSAYCAAAGIPSIVFLPADKISMAQLVQPIANGAFVLSMDTDFDGCMKLIREITAEL 302
           SAALSAYCA+AGIPSIVFLPA+KISMAQLVQPIANGAFVLS+DTDFDGCMKLIREITAEL
Sbjct: 244 SAALSAYCASAGIPSIVFLPANKISMAQLVQPIANGAFVLSIDTDFDGCMKLIREITAEL 303

Query: 303 PIYLANSLNSLRLEGQKTAAIEILQQFDWEVPDWVIIPGGNLGNIYAFYKGFQMCKELGL 362
           PIYLANSLNSLRLEGQKTAAIEILQQFDW+VPDWVI+PGGNLGNIYAFYKGF+MC+ELGL
Sbjct: 304 PIYLANSLNSLRLEGQKTAAIEILQQFDWQVPDWVIVPGGNLGNIYAFYKGFKMCQELGL 363

Query: 363 VDKIPRLVCAQAANANPLYLYYKSGWKEFSPLKASTTFASAIQIGDPVSIDRAVFALKNS 422
           VD+IPR+VCAQAANANPLYL+YKSGWK+F P+ ASTTFASAIQIGDPVSIDRAV+ALK  
Sbjct: 364 VDRIPRMVCAQAANANPLYLHYKSGWKDFKPMTASTTFASAIQIGDPVSIDRAVYALKKC 423

Query: 423 GGIVEEATEEELMDATAQADSTGMFICPHTGVALTALIKLRNRGVIKGSDRTVVVSTAHG 482
            GIVEEATEEELMDA AQADSTGMFICPHTGVALTAL KLRN+GVI  +DRTVVVSTAHG
Sbjct: 424 NGIVEEATEEELMDAMAQADSTGMFICPHTGVALTALFKLRNQGVIAPTDRTVVVSTAHG 483

Query: 483 LKFTQSKIDYHSRAIQGMECRFANPPVEVKADFGAVMDVLKEHL 526
           LKFTQSKIDYHS AI  M CRF+NPPV+VKADFGAVMDVLK +L
Sbjct: 484 LKFTQSKIDYHSNAIPDMACRFSNPPVDVKADFGAVMDVLKSYL 519

BLAST of CmaCh05G014140 vs. TAIR 10
Match: AT1G72810.1 (Pyridoxal-5'-phosphate-dependent enzyme family protein )

HSP 1 Score: 790.0 bits (2039), Expect = 1.2e-228
Identity = 398/504 (78.97%), Postives = 440/504 (87.30%), Query Frame = 0

Query: 24  LRRNSSPPFTIACPSATSDSTASPVLDSSLPLGVQNPQFA-CVDIQDDSHRSRSVAGDGG 83
           L+ +S+  FT+ C S      ASP +    P   Q P+ +   +I+D++ R         
Sbjct: 21  LKPHSAASFTVRCTS------ASPAVPPQTP---QKPRRSPDENIRDEARRRPHQL---- 80

Query: 84  RNFSAKYVPFGSGFDSSEWYSLDEIVYRSQSGGLLDVQHNMEALKEFDGAYWRNLFDSRV 143
           +N SA+YVPF +   S+E YSLDEIVYRSQSG LLDVQH+  ALK +DG +WRNLFDSRV
Sbjct: 81  QNLSARYVPFNAPPSSTESYSLDEIVYRSQSGALLDVQHDFAALKRYDGEFWRNLFDSRV 140

Query: 144 GKTTWPYGSGVWSKKEWVLPEIDSSDIVSAFEGNSNLFWAERFGKEFLGMNDLWVKHCGI 203
           GKT WPYGSGVWSKKEWVLPEID  DIVSAFEGNSNLFWAERFGK++L MNDLWVKHCGI
Sbjct: 141 GKTNWPYGSGVWSKKEWVLPEIDDDDIVSAFEGNSNLFWAERFGKQYLQMNDLWVKHCGI 200

Query: 204 SHTGSFKDLGMTVLVSQVNRLRKMNRPVVGVGCASTGDTSAALSAYCAAAGIPSIVFLPA 263
           SHTGSFKDLGM+VLVSQVNRLRKMN+PV+GVGCASTGDTSAALSAYCA+AGIPSIVFLPA
Sbjct: 201 SHTGSFKDLGMSVLVSQVNRLRKMNKPVIGVGCASTGDTSAALSAYCASAGIPSIVFLPA 260

Query: 264 DKISMAQLVQPIANGAFVLSMDTDFDGCMKLIREITAELPIYLANSLNSLRLEGQKTAAI 323
           DKISMAQLVQPIANGAFVLS+DTDFDGCM LIRE+TAELPIYLANSLNSLRLEGQKTAAI
Sbjct: 261 DKISMAQLVQPIANGAFVLSIDTDFDGCMHLIREVTAELPIYLANSLNSLRLEGQKTAAI 320

Query: 324 EILQQFDWEVPDWVIIPGGNLGNIYAFYKGFQMCKELGLVDKIPRLVCAQAANANPLYLY 383
           EILQQF+W+VPDWVI+PGGNLGNIYAFYKGF MCKELGLVD+IPRLVCAQAANANPLYL+
Sbjct: 321 EILQQFNWQVPDWVIVPGGNLGNIYAFYKGFHMCKELGLVDRIPRLVCAQAANANPLYLH 380

Query: 384 YKSGWKE-FSPLKASTTFASAIQIGDPVSIDRAVFALKNSGGIVEEATEEELMDATAQAD 443
           YKSG+KE F+PLKA+TTFASAIQIGDPVSIDRAV+ALK S GIVEEATEEELMDATA AD
Sbjct: 381 YKSGFKEDFNPLKANTTFASAIQIGDPVSIDRAVYALKKSNGIVEEATEEELMDATALAD 440

Query: 444 STGMFICPHTGVALTALIKLRNRGVIKGSDRTVVVSTAHGLKFTQSKIDYHSRAIQGMEC 503
           STGMFICPHTGVALTAL+KLR  GVI  +DRTVVVSTAHGLKFTQSKIDYHS+ I+ M C
Sbjct: 441 STGMFICPHTGVALTALMKLRKSGVIGANDRTVVVSTAHGLKFTQSKIDYHSKNIKEMAC 500

Query: 504 RFANPPVEVKADFGAVMDVLKEHL 526
           R ANPPV+VKA FG+VMDVLKE+L
Sbjct: 501 RLANPPVKVKAKFGSVMDVLKEYL 511

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9MT281.2e-23885.99Threonine synthase, chloroplastic OS=Solanum tuberosum OX=4113 PE=2 SV=1[more]
Q9S7B54.3e-23678.82Threonine synthase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=TS1 PE=1 ... [more]
Q9SSP51.6e-22778.97Threonine synthase 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=TS2 PE=1 ... [more]
Q588601.6e-7337.83Threonine synthase OS=Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 266... [more]
Q8TQD42.7e-6540.15Threonine synthase OS=Methanosarcina acetivorans (strain ATCC 35395 / DSM 2834 /... [more]
Match NameE-valueIdentityDescription
AT4G29840.13.1e-23778.82Pyridoxal-5'-phosphate-dependent enzyme family protein [more]
AT1G72810.11.2e-22878.97Pyridoxal-5'-phosphate-dependent enzyme family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036052Tryptophan synthase beta subunit-like PLP-dependent enzymeGENE3D3.40.50.1100coord: 160..486
e-value: 2.6E-107
score: 360.7
IPR036052Tryptophan synthase beta subunit-like PLP-dependent enzymeGENE3D3.40.50.1100coord: 208..309
e-value: 2.6E-107
score: 360.7
IPR036052Tryptophan synthase beta subunit-like PLP-dependent enzymeSUPERFAMILY53686Tryptophan synthase beta subunit-like PLP-dependent enzymescoord: 101..525
IPR004450Threonine synthase-likeTIGRFAMTIGR00260TIGR00260coord: 159..485
e-value: 2.6E-61
score: 205.6
IPR001926Pyridoxal-phosphate dependent enzymePFAMPF00291PALPcoord: 172..478
e-value: 1.3E-48
score: 165.9
NoneNo IPR availablePIRSRPIRSR038945-1PIRSR038945-1coord: 152..469
e-value: 8.4E-71
score: 236.6
NoneNo IPR availablePIRSRPIRSR038945-2PIRSR038945-2coord: 152..469
e-value: 8.4E-71
score: 236.6
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..24
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..17
NoneNo IPR availablePANTHERPTHR10314CYSTATHIONINE BETA-SYNTHASEcoord: 35..527
NoneNo IPR availablePANTHERPTHR10314:SF176THREONINE SYNTHASE, CHLOROPLASTIC-LIKE ISOFORM X1coord: 35..527
NoneNo IPR availableCDDcd01563Thr-synth_1coord: 154..484
e-value: 3.23077E-148
score: 426.239
IPR000634Serine/threonine dehydratase, pyridoxal-phosphate-binding sitePROSITEPS00165DEHYDRATASE_SER_THRcoord: 199..213

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh05G014140.1CmaCh05G014140.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0019344 cysteine biosynthetic process
biological_process GO:0009088 threonine biosynthetic process
biological_process GO:0006520 cellular amino acid metabolic process
cellular_component GO:0005737 cytoplasm
molecular_function GO:0030170 pyridoxal phosphate binding
molecular_function GO:0004795 threonine synthase activity