CmaCh05G013660 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh05G013660
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
DescriptionE3 ubiquitin-protein ligase RING1-like
LocationCma_Chr05: 10287784 .. 10288140 (-)
RNA-Seq ExpressionCmaCh05G013660
SyntenyCmaCh05G013660
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTGCCGTTTCTGTGTCGGCTGAGACTACGCGAAACTTAGCAGCGGCCTATCCAATTGAATTTCTGTTTGACCTCGACATGGTTCTGTCCGGCACCGACGACTTCGGTTGCTGTCAGATCAGCGCTTCGGATTCCCTTGTGGCGGATTTGCCGACGGTGGTGGCCGATGTTTGTGCCGTTTGTTTGGAGGATTTCCGGCCAGATGAGGACGGAAAACAAATCCCCTGCGGACACGTGTACCACGAATCCTGTATTTCCGCTTGGCTCGCCGTCGCCAACCGTTGCCCTCTCTGTCGGTGCCTCATCGCCGGTCAACCGCCGGAATCGCCTTCTTCAACTTTGATCCGACGGTAG

mRNA sequence

ATGGCTGCCGTTTCTGTGTCGGCTGAGACTACGCGAAACTTAGCAGCGGCCTATCCAATTGAATTTCTGTTTGACCTCGACATGGTTCTGTCCGGCACCGACGACTTCGGTTGCTGTCAGATCAGCGCTTCGGATTCCCTTGTGGCGGATTTGCCGACGGTGGTGGCCGATGTTTGTGCCGTTTGTTTGGAGGATTTCCGGCCAGATGAGGACGGAAAACAAATCCCCTGCGGACACGTGTACCACGAATCCTGTATTTCCGCTTGGCTCGCCGTCGCCAACCGTTGCCCTCTCTGTCGGTGCCTCATCGCCGGTCAACCGCCGGAATCGCCTTCTTCAACTTTGATCCGACGGTAG

Coding sequence (CDS)

ATGGCTGCCGTTTCTGTGTCGGCTGAGACTACGCGAAACTTAGCAGCGGCCTATCCAATTGAATTTCTGTTTGACCTCGACATGGTTCTGTCCGGCACCGACGACTTCGGTTGCTGTCAGATCAGCGCTTCGGATTCCCTTGTGGCGGATTTGCCGACGGTGGTGGCCGATGTTTGTGCCGTTTGTTTGGAGGATTTCCGGCCAGATGAGGACGGAAAACAAATCCCCTGCGGACACGTGTACCACGAATCCTGTATTTCCGCTTGGCTCGCCGTCGCCAACCGTTGCCCTCTCTGTCGGTGCCTCATCGCCGGTCAACCGCCGGAATCGCCTTCTTCAACTTTGATCCGACGGTAG

Protein sequence

MAAVSVSAETTRNLAAAYPIEFLFDLDMVLSGTDDFGCCQISASDSLVADLPTVVADVCAVCLEDFRPDEDGKQIPCGHVYHESCISAWLAVANRCPLCRCLIAGQPPESPSSTLIRR
Homology
[Close]
BLAST of CmaCh05G013660 vs. ExPASy Swiss-Prot
Match: Q8LPN7 (E3 ubiquitin-protein ligase RING1-like OS=Arabidopsis thaliana OX=3702 GN=At3g19950 PE=1 SV=1)

HSP 1 Score: 64.7 bits (156), Expect = 8.0e-10
Identity = 29/76 (38.16%), Postives = 42/76 (55.26%), Query Frame = 0 

Query: 43  ASDSLVADLPTVVA---------DVCAVCLEDFRPDEDGKQIPCGHVYHESCISAWLAVA 102
           AS S +  LPTV           + CAVC+++F    D KQ+PC HV+H+ C+  WL + 
Sbjct: 191 ASKSAIDALPTVKVTKDMLKSEMNQCAVCMDEFEDGSDVKQMPCKHVFHQDCLLPWLELH 250

Query: 103 NRCPLCRCLIAGQPPE 110
           N CP+CR  +    P+
Sbjct: 251 NSCPVCRFELPTDDPD 266

[Close]
BLAST of CmaCh05G013660 vs. ExPASy Swiss-Prot
Match: Q940T5 (E3 ubiquitin-protein ligase RDUF2 OS=Arabidopsis thaliana OX=3702 GN=DURF2 PE=1 SV=1)

HSP 1 Score: 63.2 bits (152), Expect = 2.3e-09
Identity = 27/73 (36.99%), Postives = 43/73 (58.90%), Query Frame = 0 

Query: 43  ASDSLVADLPTV-VADV-------CAVCLEDFRPDEDGKQIPCGHVYHESCISAWLAVAN 102
           AS S +  LP V ++D        CAVC E F  + + +++PC H++H+ CI  WL++ N
Sbjct: 175 ASKSAIESLPRVEISDCHIGSEANCAVCTEIFETETEAREMPCKHLFHDDCIVPWLSIRN 234

Query: 103 RCPLCRCLIAGQP 108
            CP+CR  +  +P
Sbjct: 235 SCPVCRFELPSEP 247

[Close]
BLAST of CmaCh05G013660 vs. ExPASy Swiss-Prot
Match: P0CH30 (E3 ubiquitin-protein ligase RING1 OS=Gossypium hirsutum OX=3635 GN=RING1 PE=1 SV=1)

HSP 1 Score: 63.2 bits (152), Expect = 2.3e-09
Identity = 22/51 (43.14%), Postives = 33/51 (64.71%), Query Frame = 0 

Query: 59  CAVCLEDFRPDEDGKQIPCGHVYHESCISAWLAVANRCPLCRCLIAGQPPE 110
           CAVC++DF    + KQ+PC H+YH+ C+  WL + N CP+CR  +    P+
Sbjct: 224 CAVCMDDFEEGTEAKQMPCKHLYHKDCLLPWLELHNSCPVCRHELPTDDPD 274

[Close]
BLAST of CmaCh05G013660 vs. ExPASy Swiss-Prot
Match: Q9SNB6 (E3 ubiquitin-protein ligase RDUF1 OS=Arabidopsis thaliana OX=3702 GN=RDUF1 PE=1 SV=1)

HSP 1 Score: 62.0 bits (149), Expect = 5.2e-09
Identity = 27/70 (38.57%), Postives = 40/70 (57.14%), Query Frame = 0 

Query: 42  SASDSL----VADLPTVVADVCAVCLEDFRPDEDGKQIPCGHVYHESCISAWLAVANRCP 101
           SA +SL    ++D  T     CAVC E F    +G+++PC H++H  CI  WL++ N CP
Sbjct: 194 SAIESLPRVEISDCHTKAEANCAVCTEVFEAGIEGREMPCKHIFHGDCIVPWLSIRNSCP 253

Query: 102 LCRCLIAGQP 108
           +CR  +   P
Sbjct: 254 VCRFELPSDP 263

[Close]
BLAST of CmaCh05G013660 vs. ExPASy Swiss-Prot
Match: Q9VHI7 (E3 ubiquitin-protein ligase Iruka OS=Drosophila melanogaster OX=7227 GN=Iru PE=1 SV=1)

HSP 1 Score: 61.6 bits (148), Expect = 6.8e-09
Identity = 21/46 (45.65%), Postives = 34/46 (73.91%), Query Frame = 0 

Query: 59  CAVCLEDFRPDEDGKQIPCGHVYHESCISAWLAVANRCPLCRCLIA 105
           C++C +DF+ DE  +++PC H+YHE+CI  WL + + CP+CR  +A
Sbjct: 253 CSICWDDFKIDETVRKLPCSHLYHENCIVPWLNLHSTCPICRKSLA 298

[Close]
BLAST of CmaCh05G013660 vs. TAIR 10
Match: AT1G68180.1 (RING/U-box superfamily protein )

HSP 1 Score: 67.0 bits (162), Expect = 1.1e-11
Identity = 25/54 (46.30%), Postives = 36/54 (66.67%), Query Frame = 0 

Query: 47  LVADLPTVVADVCAVCLEDFRPDEDGKQIPCGHVYHESCISAWLAVANRCPLCR 101
           ++ D   V   VCA+C E+F   E+GK++ C H+YH SCI +WL + N CP+CR
Sbjct: 126 IITDEDLVKEKVCAICKEEFEVGEEGKELKCLHLYHSSCIVSWLNIHNTCPICR 179

[Close]
BLAST of CmaCh05G013660 vs. TAIR 10
Match: AT3G19950.1 (RING/U-box superfamily protein )

HSP 1 Score: 64.7 bits (156), Expect = 5.7e-11
Identity = 29/76 (38.16%), Postives = 42/76 (55.26%), Query Frame = 0 

Query: 43  ASDSLVADLPTVVA---------DVCAVCLEDFRPDEDGKQIPCGHVYHESCISAWLAVA 102
           AS S +  LPTV           + CAVC+++F    D KQ+PC HV+H+ C+  WL + 
Sbjct: 191 ASKSAIDALPTVKVTKDMLKSEMNQCAVCMDEFEDGSDVKQMPCKHVFHQDCLLPWLELH 250

Query: 103 NRCPLCRCLIAGQPPE 110
           N CP+CR  +    P+
Sbjct: 251 NSCPVCRFELPTDDPD 266

[Close]
BLAST of CmaCh05G013660 vs. TAIR 10
Match: AT5G59550.1 (zinc finger (C3HC4-type RING finger) family protein )

HSP 1 Score: 63.2 bits (152), Expect = 1.7e-10
Identity = 27/73 (36.99%), Postives = 43/73 (58.90%), Query Frame = 0 

Query: 43  ASDSLVADLPTV-VADV-------CAVCLEDFRPDEDGKQIPCGHVYHESCISAWLAVAN 102
           AS S +  LP V ++D        CAVC E F  + + +++PC H++H+ CI  WL++ N
Sbjct: 175 ASKSAIESLPRVEISDCHIGSEANCAVCTEIFETETEAREMPCKHLFHDDCIVPWLSIRN 234

Query: 103 RCPLCRCLIAGQP 108
            CP+CR  +  +P
Sbjct: 235 SCPVCRFELPSEP 247

[Close]
BLAST of CmaCh05G013660 vs. TAIR 10
Match: AT3G60080.1 (RING/U-box superfamily protein )

HSP 1 Score: 62.4 bits (150), Expect = 2.8e-10
Identity = 23/43 (53.49%), Postives = 29/43 (67.44%), Query Frame = 0 

Query: 58  VCAVCLEDFRPDEDGKQIPCGHVYHESCISAWLAVANRCPLCR 101
           +CAVC EDF   E  +++PC H+YH  CI  WL+  N CPLCR
Sbjct: 168 LCAVCKEDFIIGESARRLPCSHIYHSDCIVPWLSDHNSCPLCR 210

[Close]
BLAST of CmaCh05G013660 vs. TAIR 10
Match: AT3G46620.1 (zinc finger (C3HC4-type RING finger) family protein )

HSP 1 Score: 62.0 bits (149), Expect = 3.7e-10
Identity = 27/70 (38.57%), Postives = 40/70 (57.14%), Query Frame = 0 

Query: 42  SASDSL----VADLPTVVADVCAVCLEDFRPDEDGKQIPCGHVYHESCISAWLAVANRCP 101
           SA +SL    ++D  T     CAVC E F    +G+++PC H++H  CI  WL++ N CP
Sbjct: 194 SAIESLPRVEISDCHTKAEANCAVCTEVFEAGIEGREMPCKHIFHGDCIVPWLSIRNSCP 253

Query: 102 LCRCLIAGQP 108
           +CR  +   P
Sbjct: 254 VCRFELPSDP 263

The following BLAST results are available for this feature:
BLAST of CmaCh05G013660 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q8LPN78.0e-1038.16E3 ubiquitin-protein ligase RING1-like OS=Arabidopsis thaliana OX=3702 GN=At3g19... [more]
Q940T52.3e-0936.99E3 ubiquitin-protein ligase RDUF2 OS=Arabidopsis thaliana OX=3702 GN=DURF2 PE=1 ... [more]
P0CH302.3e-0943.14E3 ubiquitin-protein ligase RING1 OS=Gossypium hirsutum OX=3635 GN=RING1 PE=1 SV... [more]
Q9SNB65.2e-0938.57E3 ubiquitin-protein ligase RDUF1 OS=Arabidopsis thaliana OX=3702 GN=RDUF1 PE=1 ... [more]
Q9VHI76.8e-0945.65E3 ubiquitin-protein ligase Iruka OS=Drosophila melanogaster OX=7227 GN=Iru PE=1... [more]
BLAST of CmaCh05G013660 vs. TAIR 10
Match NameE-valueIdentityDescription
AT1G68180.11.1e-1146.30RING/U-box superfamily protein [more]
AT3G19950.15.7e-1138.16RING/U-box superfamily protein [more]
AT5G59550.11.7e-1036.99zinc finger (C3HC4-type RING finger) family protein [more]
AT3G60080.12.8e-1053.49RING/U-box superfamily protein [more]
AT3G46620.13.7e-1038.57zinc finger (C3HC4-type RING finger) family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001841Zinc finger, RING-typeSMARTSM00184ring_2coord: 59..99
e-value: 1.3E-8
score: 44.6
IPR001841Zinc finger, RING-typePFAMPF13639zf-RING_2coord: 58..100
e-value: 2.6E-13
score: 50.1
IPR001841Zinc finger, RING-typePROSITEPS50089ZF_RING_2coord: 59..100
score: 12.99088
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 53..113
e-value: 1.5E-17
score: 64.5
NoneNo IPR availablePANTHERPTHR15710E3 UBIQUITIN-PROTEIN LIGASE PRAJAcoord: 21..103
NoneNo IPR availablePANTHERPTHR15710:SF74E3 UBIQUITIN-PROTEIN LIGASE RING1-LIKEcoord: 21..103
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 58..106

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh05G013660.1CmaCh05G013660.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016567 protein ubiquitination
biological_process GO:0006511 ubiquitin-dependent protein catabolic process
cellular_component GO:0005737 cytoplasm
molecular_function GO:0061630 ubiquitin protein ligase activity