CmaCh05G012050 (gene) Cucurbita maxima (Rimu) v1.1

Overview


Name	CmaCh05G012050
Type	gene
Organism	Cucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Description	pathogenesis-related protein PR-4-like
Location	Cma_Chr05: 9309763 .. 9310298 (+)
RNA-Seq Expression	CmaCh05G012050
Synteny	CmaCh05G012050

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

AGAGAATCAAGAAAGAAAAGAATGGAGAAAGGGAGCTTTTTGATGATATTAGCTTTGGCTTTGGGCATATTGAGCTTGGCTAATGCTCAAAGTGCCAACAATGTGAGGGCTACTTACCATTTGTACAACCCACAAGACATCAATTGGGACTACCTTAGAGCAAGTGTTTTTTGTGCAACATGGGATGCTAACAAGCCCATCGAGTGGCGTCGTCAGTACGGTTGGACTGCCTTCTGTGGCCCTGTCGGCCCTCGTGGCCAAGCTTCTTGTGGACGTTGCTTGAGGGTAAGTAAATCTTCTAGTTCTTGCAAAAAATTATCAGATTTTAGAGTTACAAATGTGCTGAGAAATTTTCACGGGAATGTTTGCAGGTCACTAACACTGAAACAGGAGCTTCGGAAATTGTGAGAATCGTTGATCAATGTGCCAATGGAGGGCTTGATTTGGATGTGAATGTGTTTAAGAGAATCGACACCAATGGAAATGGAGTCTTCCGAGGCCATCTTATTGTCAACTATCAGTTTGTCAATTGTTAA

mRNA sequence

AGAGAATCAAGAAAGAAAAGAATGGAGAAAGGGAGCTTTTTGATGATATTAGCTTTGGCTTTGGGCATATTGAGCTTGGCTAATGCTCAAAGTGCCAACAATGTGAGGGCTACTTACCATTTGTACAACCCACAAGACATCAATTGGGACTACCTTAGAGCAAGTGTTTTTTGTGCAACATGGGATGCTAACAAGCCCATCGAGTGGCGTCGTCAGTACGGTTGGACTGCCTTCTGTGGCCCTGTCGGCCCTCGTGGCCAAGCTTCTTGTGGACGTTGCTTGAGGGTCACTAACACTGAAACAGGAGCTTCGGAAATTGTGAGAATCGTTGATCAATGTGCCAATGGAGGGCTTGATTTGGATGTGAATGTGTTTAAGAGAATCGACACCAATGGAAATGGAGTCTTCCGAGGCCATCTTATTGTCAACTATCAGTTTGTCAATTGTTAA

Coding sequence (CDS)

ATGGAGAAAGGGAGCTTTTTGATGATATTAGCTTTGGCTTTGGGCATATTGAGCTTGGCTAATGCTCAAAGTGCCAACAATGTGAGGGCTACTTACCATTTGTACAACCCACAAGACATCAATTGGGACTACCTTAGAGCAAGTGTTTTTTGTGCAACATGGGATGCTAACAAGCCCATCGAGTGGCGTCGTCAGTACGGTTGGACTGCCTTCTGTGGCCCTGTCGGCCCTCGTGGCCAAGCTTCTTGTGGACGTTGCTTGAGGGTCACTAACACTGAAACAGGAGCTTCGGAAATTGTGAGAATCGTTGATCAATGTGCCAATGGAGGGCTTGATTTGGATGTGAATGTGTTTAAGAGAATCGACACCAATGGAAATGGAGTCTTCCGAGGCCATCTTATTGTCAACTATCAGTTTGTCAATTGTTAA

Protein sequence

MEKGSFLMILALALGILSLANAQSANNVRATYHLYNPQDINWDYLRASVFCATWDANKPIEWRRQYGWTAFCGPVGPRGQASCGRCLRVTNTETGASEIVRIVDQCANGGLDLDVNVFKRIDTNGNGVFRGHLIVNYQFVNC

Homology

[Close]

BLAST of CmaCh05G012050 vs. ExPASy Swiss-Prot
Match: P32045 (Pathogenesis-related protein P2 OS=Solanum lycopersicum OX=4081 PE=2 SV=1)

HSP 1 Score: 220.3 bits (560), Expect = 1.4e-56
Identity = 98/127 (77.17%), Postives = 110/127 (86.61%), Query Frame = 0


Query: 16  ILSLANAQSANNVRATYHLYNPQDINWDYLRASVFCATWDANKPIEWRRQYGWTAFCGPV 75
           ++++A AQSA NVRATYHLYNPQ+INWD   ASV+CATWDA+KP+EWRR+YGWTAFCGP 
Sbjct: 17  MMAVAAAQSATNVRATYHLYNPQNINWDLRTASVYCATWDADKPLEWRRRYGWTAFCGPA 76

Query: 76  GPRGQASCGRCLRVTNTETGASEIVRIVDQCANGGLDLDVNVFKRIDTNGNGVFRGHLIV 135
           GP GQASCGRCLRVTNT TG  E VRIVDQC NGGLDLDVNVF R+DTNG G  RG+L V
Sbjct: 77  GPTGQASCGRCLRVTNTGTGTQETVRIVDQCRNGGLDLDVNVFNRLDTNGLGYQRGNLNV 136

Query: 136 NYQFVNC 143
           NY+FVNC
Sbjct: 137 NYEFVNC 143

[Close]

BLAST of CmaCh05G012050 vs. ExPASy Swiss-Prot
Match: P29063 (Pathogenesis-related protein PR-4B OS=Nicotiana tabacum OX=4097 PE=2 SV=1)

HSP 1 Score: 216.1 bits (549), Expect = 2.6e-55
Identity = 94/127 (74.02%), Postives = 109/127 (85.83%), Query Frame = 0


Query: 16  ILSLANAQSANNVRATYHLYNPQDINWDYLRASVFCATWDANKPIEWRRQYGWTAFCGPV 75
           ++++A AQSA NVR+TYHLYNPQ+INWD   AS FCATWDA+KP+ WR++YGWTAFCGP 
Sbjct: 19  MMAMAAAQSATNVRSTYHLYNPQNINWDLRAASAFCATWDADKPLAWRQKYGWTAFCGPA 78

Query: 76  GPRGQASCGRCLRVTNTETGASEIVRIVDQCANGGLDLDVNVFKRIDTNGNGVFRGHLIV 135
           GPRGQ SCGRCLRVTNT TG    VRIVDQC+NGGLDLDVNVF ++DTNG G  +GHLIV
Sbjct: 79  GPRGQDSCGRCLRVTNTGTGTQATVRIVDQCSNGGLDLDVNVFNQLDTNGLGYQQGHLIV 138

Query: 136 NYQFVNC 143
           NY+FVNC
Sbjct: 139 NYEFVNC 145

[Close]

BLAST of CmaCh05G012050 vs. ExPASy Swiss-Prot
Match: P29062 (Pathogenesis-related protein PR-4A OS=Nicotiana tabacum OX=4097 PE=2 SV=1)

HSP 1 Score: 213.8 bits (543), Expect = 1.3e-54
Identity = 95/136 (69.85%), Postives = 114/136 (83.82%), Query Frame = 0


Query: 7   LMILALALGILSLANAQSANNVRATYHLYNPQDINWDYLRASVFCATWDANKPIEWRRQY 66
           L+I+++   ++++A AQSA NVR+TYHLYNPQ+INWD   AS FCATWDA+KP+ WR++Y
Sbjct: 13  LLIISM---VMAMAAAQSATNVRSTYHLYNPQNINWDLRAASAFCATWDADKPLAWRQKY 72

Query: 67  GWTAFCGPVGPRGQASCGRCLRVTNTETGASEIVRIVDQCANGGLDLDVNVFKRIDTNGN 126
           GWTAFCGP GPRGQ SCGRCLRVTNT TG    VRIVDQC+NGGLDLDVNVF ++DTNG 
Sbjct: 73  GWTAFCGPAGPRGQDSCGRCLRVTNTGTGTQTTVRIVDQCSNGGLDLDVNVFNQLDTNGV 132

Query: 127 GVFRGHLIVNYQFVNC 143
           G  +GHL VNY+FVNC
Sbjct: 133 GYQQGHLTVNYEFVNC 145

[Close]

BLAST of CmaCh05G012050 vs. ExPASy Swiss-Prot
Match: P09761 (Wound-induced protein WIN1 OS=Solanum tuberosum OX=4113 GN=WIN1 PE=2 SV=1)

HSP 1 Score: 209.5 bits (532), Expect = 2.4e-53
Identity = 88/119 (73.95%), Postives = 104/119 (87.39%), Query Frame = 0


Query: 24  SANNVRATYHLYNPQDINWDYLRASVFCATWDANKPIEWRRQYGWTAFCGPVGPRGQASC 83
           SA NVRATYH+YNPQ++ WD    S +C+TWDANKP+ WR++YGWTAFCGPVGPRG+ SC
Sbjct: 79  SAQNVRATYHIYNPQNVGWDLNAVSAYCSTWDANKPLSWRKKYGWTAFCGPVGPRGRDSC 138

Query: 84  GRCLRVTNTETGASEIVRIVDQCANGGLDLDVNVFKRIDTNGNGVFRGHLIVNYQFVNC 143
           G+CLRVTNT TGA   VRIVDQC+NGGLDLDVNVF++IDT+GNG  +GHLIVNYQFV+C
Sbjct: 139 GKCLRVTNTRTGAQTTVRIVDQCSNGGLDLDVNVFRQIDTDGNGNHQGHLIVNYQFVDC 197

[Close]

BLAST of CmaCh05G012050 vs. ExPASy Swiss-Prot
Match: P09762 (Wound-induced protein WIN2 OS=Solanum tuberosum OX=4113 GN=WIN2 PE=2 SV=1)

HSP 1 Score: 203.8 bits (517), Expect = 1.3e-51
Identity = 87/119 (73.11%), Postives = 101/119 (84.87%), Query Frame = 0


Query: 24  SANNVRATYHLYNPQDINWDYLRASVFCATWDANKPIEWRRQYGWTAFCGPVGPRGQASC 83
           SA NVRATYH+YNPQ++ WD    S +C+TWDANKP  WR +YGWTAFCGPVGPRG+ SC
Sbjct: 78  SAQNVRATYHIYNPQNVGWDLNAVSAYCSTWDANKPYAWRSKYGWTAFCGPVGPRGRDSC 137

Query: 84  GRCLRVTNTETGASEIVRIVDQCANGGLDLDVNVFKRIDTNGNGVFRGHLIVNYQFVNC 143
           G+CLRVTNT TGA   VRIVDQC+NGGLDLD+NVF++IDT+G G  +GHLIVNYQFVNC
Sbjct: 138 GKCLRVTNTRTGAQTTVRIVDQCSNGGLDLDINVFQQIDTDGVGNQQGHLIVNYQFVNC 196

[Close]

BLAST of CmaCh05G012050 vs. TAIR 10
Match: AT3G04720.1 (pathogenesis-related 4 )

HSP 1 Score: 190.3 bits (482), Expect = 1.1e-48
Identity = 82/120 (68.33%), Postives = 97/120 (80.83%), Query Frame = 0


Query: 23  QSANNVRATYHLYNPQDINWDYLRASVFCATWDANKPIEWRRQYGWTAFCGPVGPRGQAS 82
           +SA+NVRATYH YNP   NWD    S +C+TWDA+KP  WR +YGWTAFCGP GPRGQAS
Sbjct: 72  ESASNVRATYHFYNPAQNNWDLRAVSAYCSTWDADKPYAWRSKYGWTAFCGPAGPRGQAS 131

Query: 83  CGRCLRVTNTETGASEIVRIVDQCANGGLDLDVNVFKRIDTNGNGVFRGHLIVNYQFVNC 142
           CG+CLRV NT T A+  VRIVDQC+NGGLDLDV +F +IDT+G G  +GHLIV+YQFV+C
Sbjct: 132 CGKCLRVKNTRTNAAVTVRIVDQCSNGGLDLDVAMFNQIDTDGFGYQQGHLIVDYQFVDC 191

The following BLAST results are available for this feature:

BLAST of CmaCh05G012050 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P32045	1.4e-56	77.17	Pathogenesis-related protein P2 OS=Solanum lycopersicum OX=4081 PE=2 SV=1	[more]
P29063	2.6e-55	74.02	Pathogenesis-related protein PR-4B OS=Nicotiana tabacum OX=4097 PE=2 SV=1	[more]
P29062	1.3e-54	69.85	Pathogenesis-related protein PR-4A OS=Nicotiana tabacum OX=4097 PE=2 SV=1	[more]
P09761	2.4e-53	73.95	Wound-induced protein WIN1 OS=Solanum tuberosum OX=4113 GN=WIN1 PE=2 SV=1	[more]
P09762	1.3e-51	73.11	Wound-induced protein WIN2 OS=Solanum tuberosum OX=4113 GN=WIN2 PE=2 SV=1	[more]

BLAST of CmaCh05G012050 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G04720.1	1.1e-48	68.33	pathogenesis-related 4	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001153	Barwin domain	PRINTS	PR00602	BARWIN	coord: 117..141 score: 66.4 coord: 58..76 score: 74.74 coord: 96..114 score: 77.19 coord: 77..95 score: 75.79
IPR001153	Barwin domain	PFAM	PF00967	Barwin	coord: 24..142 e-value: 2.1E-58 score: 195.4
IPR001153	Barwin domain	PROSITE	PS51174	BARWIN_3	coord: 23..142 score: 79.686279
IPR036908	RlpA-like domain superfamily	GENE3D	2.40.40.10		coord: 23..142 e-value: 7.3E-56 score: 189.2
IPR036908	RlpA-like domain superfamily	SUPERFAMILY	50685	Barwin-like endoglucanases	coord: 24..142
None	No IPR available	PANTHER	PTHR46351:SF6	PATHOGENESIS-RELATED PROTEIN PR-4A	coord: 8..142
IPR044301	Pathogenesis-related protein-4	PANTHER	PTHR46351	WOUND-INDUCED PROTEIN WIN2	coord: 8..142
IPR018226	Barwin, conserved site	PROSITE	PS00772	BARWIN_2	coord: 135..142
IPR018226	Barwin, conserved site	PROSITE	PS00771	BARWIN_1	coord: 83..91

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmaCh05G012050.1	CmaCh05G012050.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0042742	defense response to bacterium
biological_process	GO:0050832	defense response to fungus
biological_process	GO:0090501	RNA phosphodiester bond hydrolysis
biological_process	GO:0006952	defense response
molecular_function	GO:0004540	ribonuclease activity